LOCUS       DASUBP010000001         3053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_926699, whole genome shotgun sequence.
ACCESSION   DASUBP010000001 DASUBP010000000
VERSION     DASUBP010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3053
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_926699"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..653
                     /locus_tag="VFU75_00005"
     CDS             <1..653
                     /locus_tag="VFU75_00005"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU5465237.1"
                     /translation="ARFRPKAADRVLYLAHWDTRPIADQDQNLGNQRKPVPGANDGAS
                     GVAVLLGVADVLKRHPPAVGVDLLFVDGEDYGDFDTATLDSSFSDVLMGSRYFAKHLP
                     DPKYTPMMAVLFDMIGAKNLVIPVEQYSQAFAPEVVDRVWRVADSLGYGKVFLKQSGP
                     SLIDDHTMLQHAGIHAIDMVDFDGYHAYWHTVDDTIDKVSAESLQMVGDVAVALVR"
     gene            837..1247
                     /locus_tag="VFU75_00010"
     CDS             837..1247
                     /locus_tag="VFU75_00010"
                     /inference="COORDINATES: protein motif:HMM:NF024245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HEU5465238.1"
                     /translation="MDDKRAAFLLGLLLLGGAGTRFALAPRAPQAPGDISLVPANDSP
                     PVSPRDVAHQALRNARPLQPGERIDVNQANAVELTRLPRVGPALAGRIVQWRTAHGPF
                     HSLAALDSVPGVGPKLLEGLRNAITFSGEVPPAP"
     gene            1293..3002
                     /gene="pilB"
                     /locus_tag="VFU75_00015"
     CDS             1293..3002
                     /gene="pilB"
                     /locus_tag="VFU75_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV-A pilus assembly ATPase PilB"
                     /protein_id="HEU5465239.1"
                     /translation="MHTAVPAATTEKLGDILLREGLISPDQLKKALAEQKASGMRLGY
                     TLVKLGFVEETEITKMLARQYRMPAVDLSRFEVDPKILKLIPGDIAAKHTVLPLKREG
                     RTLTIAIGDPNNVTAIEDIKFITRCDIFPVIAGEYTLRNAIDKYYQQSDAQLQNLLKS
                     VEAEEDLEVVEEQSDEDVKKEDLADDAPVVKLINGILADAVTRGASDIHIECFEHELR
                     VRYRVDGALQEVMKPPIKWRAALISRVKIMAALNIAERRVPQDGRIKLKMGSRVIDYR
                     VSTLPVLFGEKIVLRILDKGNLTLDLAKFGFEPKAEADLMKAILNPYGMVLVTGPTGS
                     GKTTTLYSALSKINTIDVNIMTAEDPVEYNLMGINQVLVRNEVGMTFAAALKAFLRQD
                     PNIIMVGEIRDLETGSIGIKAALTGHLVLSTLHTNDAPSTITRMVDMGIEPFNVASAV
                     NLIVAQRLVRRICKDCKAEHNYTDEEMHAFGVAREEGPFFKGAGCDTCNGTGYKGRQG
                     LYEVMALSSPLRRGILKGASTEELREIAVSEGMLTLRMDGMVKVKKGITTLEEIVKET
                     AAN"
BASE COUNT          590 a          986 c          981 g          496 t
ORIGIN      
        1 tcgcccgctt ccgccccaag gccgccgacc gcgtcctcta cctggcgcac tgggacaccc
       61 gacccatcgc cgatcaggac cagaacctcg gcaaccagcg gaagccggtg cccggggcga
      121 acgacggcgc gtccggcgta gccgtgctcc tgggggtcgc ggacgtcctg aagcggcacc
      181 cgcccgccgt cggcgtcgac ctgctgttcg tggacggaga ggactacggc gacttcgaca
      241 cggcgaccct cgattcttca ttctcggacg tcctgatggg gtcccggtac ttcgccaagc
      301 atttacccga tccgaagtac acgcccatga tggcggtgct cttcgacatg atcggggcca
      361 agaacctcgt cattcccgtg gagcagtact cccaggcctt cgcgcccgag gtggtcgacc
      421 gggtctggcg cgtcgccgac tccctcggct acggcaaggt cttcctcaag caatccggac
      481 cctccctcat cgacgaccac accatgctcc agcatgccgg catccacgcc atcgacatgg
      541 tggacttcga cgggtatcac gcctactggc acacggtcga cgacacgatc gacaaggtct
      601 cggctgagtc gctgcagatg gtgggggatg tggcggtggc gctcgtgagg taaatgccgg
      661 gagacgtgag ccgggagccg tcgcagtcca ggcgagggaa tctccgactt ccgagggggt
      721 ccgttcgtgg ctccgactca gacggctccc ctctcccggc tcccgtgcgt tcaggggatg
      781 tgtagcacgt tattcgggtg atggtcgtgg gcatcgaagt ggttaggttg ccggccatgg
      841 acgacaaacg cgcagctttc cttctcggtc ttctccttct cggcggcgcc ggaacccggt
      901 ttgcgctggc gcctagggcc ccgcaggcac cgggggacat ctccctcgtc cccgccaacg
      961 attcgccccc agtgtccccg cgagacgtcg cgcaccaggc gctccgaaat gcgcggccgc
     1021 tgcaacccgg cgaacgcatc gacgtcaatc aggccaacgc ggtggagctg acgcgactcc
     1081 cgcgggtggg tccggcactc gcggggcgca tcgtgcagtg gcgcaccgcg catggcccgt
     1141 ttcactcact cgcggcgctc gattcggttc cgggggtggg tcccaagctg ttggagggct
     1201 tgcggaacgc gatcaccttc tcgggggagg tgcctcccgc gccttgacac acccaccccc
     1261 caaagtatgt tagatgcttc aaccacaacg agttgcatac ggcagtcccg gcggcaacca
     1321 ccgaaaaact gggcgatatc ctcctccgtg aggggctgat ctccccggat cagctcaaga
     1381 aggccctggc cgaacagaag gccagtggga tgcgcctggg ctacaccttg gtaaagctcg
     1441 gtttcgtcga agaaaccgag atcaccaaga tgctggcccg gcaataccgg atgccggcgg
     1501 tcgacctctc ccgcttcgaa gtcgatccca agatcctgaa gctgatcccc ggagatatcg
     1561 cggcgaagca cacggtcctg ccgctcaagc gggagggccg caccctcacg atcgccatcg
     1621 gggacccgaa caacgtcacc gcgatcgagg acatcaagtt catcacccgc tgcgacatct
     1681 tcccggtcat cgcgggcgag tacaccctcc ggaacgcgat cgacaagtac taccagcagt
     1741 cggatgccca gctccagaac ctcctgaaga gcgtcgaagc ggaagaagac ctcgaggtgg
     1801 tggaggagca gtccgacgaa gacgtcaaga aggaagacct ggccgatgac gcgccggtcg
     1861 tgaagctgat caacggcatt ctggccgacg cggtgacccg cggcgcgtcc gacattcaca
     1921 tcgagtgctt cgagcacgag ctccgcgtgc gctaccgggt ggacggtgct cttcaagagg
     1981 tgatgaagcc gccgatcaag tggcgcgcgg cgctgatctc gcgcgtcaag atcatggcgg
     2041 cgctgaacat cgcggaacgg cgggtgccgc aggacggccg catcaagctg aagatgggct
     2101 cgcgggtgat cgactaccgc gtttccacgt tgcccgtgct gttcggcgag aagatcgtcc
     2161 tccggatcct ggacaagggg aacctgacgc tcgatctcgc caagttcggc ttcgagccca
     2221 aggccgaagc ggacttgatg aaggccattc tgaatccgta cgggatggtg ctggtgacgg
     2281 gcccgacggg gtcaggcaag accacgaccc tctactcggc cctgtcgaag atcaacacca
     2341 tcgacgtcaa catcatgacc gcggaagacc ccgtcgaata caacctgatg gggatcaacc
     2401 aggtgctggt gcggaacgaa gtcggcatga cgttcgccgc ggcactgaag gccttcctgc
     2461 ggcaggaccc gaacatcatc atggtcggcg agatccggga cttggagacg ggctccatcg
     2521 gcatcaaggc ggccctcacg ggccacctcg tgctgtccac gctgcacacg aacgacgcgc
     2581 cctcgaccat cacccgtatg gtggacatgg ggatcgagcc gttcaacgtg gcgagcgcgg
     2641 tgaacctgat cgtggcgcag cggctggtcc gacggatctg caaggactgc aaggcggagc
     2701 acaactacac cgacgaagag atgcacgcgt tcggcgtcgc ccgcgaagag ggcccgttct
     2761 tcaagggcgc cggctgcgac acgtgcaacg gcaccgggta caaagggcgg caggggctct
     2821 acgaggtgat ggcgctgtcc tcaccgctcc ggcgggggat cctgaaaggc gcgtccacgg
     2881 aggagctgcg ggaaatcgcg gtcagcgagg gcatgctcac cctgcgcatg gacggcatgg
     2941 tgaaggtgaa gaaaggcatc accacgctcg aggaaatcgt gaaggaaacg gcggccaatt
     3001 gaaggagact agttcatgcc ggtgaatctg cgcgccctcc tcgaagagat gat
//
LOCUS       DASUBP010000002         2820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_286193, whole genome shotgun sequence.
ACCESSION   DASUBP010000002 DASUBP010000000
VERSION     DASUBP010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2820
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_286193"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /gene="uppS"
                     /locus_tag="VFU75_00020"
     CDS             <1..579
                     /gene="uppS"
                     /locus_tag="VFU75_00020"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEU5465240.1"
                     /translation="AIESGVEVLTLFAFSEENWNRPATEISALMNLLEEYIAREVREL
                     SDQGVRVNVLGDLTRLGPKQRAAVERIMADTRSGTKLSLNLCISYSSRAELARAARLL
                     AEEVLKGNKRLEDLDEGALASKLYTAQWPDPDLLIRTSGEQRLSNFLLWQLAYTEIYI
                     TPVLWPDFDRQCLYEAILDYQRRERRFGNVTA"
     gene            579..1412
                     /locus_tag="VFU75_00025"
     CDS             579..1412
                     /locus_tag="VFU75_00025"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEU5465241.1"
                     /translation="MSRNLVVRIAFAVVAIPLAVLIIYEGDWILAAMLAVLGVLGTRE
                     LYDLGRKQGIEPIAWLGYLCAAAIPLGMLSSMIVGWPYALACWLMAVLVVALATRTPA
                     QKPLTAVALTVLGPFYASWMLAFILKLRSGALGPWQGTWLVLLPLVATWICDTAAMGA
                     GSAIGGPKLAPVVSPKKTWAGAIGGLIGAIAATVAIGLWVFPRLGMAVGVLPLVVIGV
                     AIGILAQVGDVAESLFKREAGVKDSSSLIPGHGGVLDRLDSLYFVLPAAAGLFWFFRI
                     P"
     gene            1409..2521
                     /gene="dxr"
                     /locus_tag="VFU75_00030"
     CDS             1409..2521
                     /gene="dxr"
                     /locus_tag="VFU75_00030"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HEU5465242.1"
                     /translation="MTTRSAGVAILGSTGSIGRSTLQVLSRQRDRFRLVALTAHTNQA
                     LLDAQAKEWKPAFAKCVNGNGRECLIEAATRPDVDIVVNGIVGAAGLEATLAALSAGK
                     RVALANKETLVMAGELVTAAAKAGGGELVPVDSEHSAVLQCLSGRTPDDLRRLILTAS
                     GGPFRTWTPSQVAAASVEQALQHPTWKMGPKITVDSATLVNKALEVIEAHFLFGLAYP
                     SVEVVVHPQSIIHAFVEFRDGSVLAQVGFPTMELPILYALTHPERVEDSGTRAFDPLA
                     TGDLTFQPVNPALFPAYELGRGAGMAGGTAPAVFNAANEVAVELFLSGTIRFGRIAAV
                     IEGTLSKAPAGDAGTLDGVLHADREARRLAREIACS"
     gene            2512..>2820
                     /locus_tag="VFU75_00035"
     CDS             2512..>2820
                     /locus_tag="VFU75_00035"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU5465243.1"
                     /translation="MLLTVAAFVFVLGVLIFVHEAGHFLVAKATGVQVLRFSLGFGKP
                     LVKFTWGETEYVIAYLPFGGYVKMAGLEEEGMMGEVEGGAATVPVDPERAFDRKPLVV
                     R"
BASE COUNT          407 a          951 c          968 g          494 t
ORIGIN      
        1 gccatcgaga gcggcgtcga ggtgctcacg ctcttcgcgt tctcggaaga gaactggaac
       61 cgccccgcca ccgagatctc cgccctgatg aacctgctcg aggagtacat cgcccgggag
      121 gtccgggagc tctccgacca gggcgtgcgc gtgaacgtgc tcggcgacct gacccggctc
      181 ggccccaagc agcgggcggc ggtcgagcgg atcatggcgg acacgcggag cggcaccaag
      241 ctctccctca acctctgcat ctcctattcg tcccgcgccg agctggcgcg cgcggcacgg
      301 ctcctggccg aggaagtcct gaagggcaac aagcgcctgg aagatctcga tgagggcgcg
      361 ctggcctcca agctctacac ggcccagtgg cccgatcccg acctcctgat ccgcacctcc
      421 ggggagcagc ggctctccaa cttcctgctc tggcagctgg cgtacaccga gatctacatc
      481 acgccggtgc tctggcccga cttcgaccgc cagtgcctct acgaggcgat cctcgactat
      541 cagcggcgcg agcggcgctt cggtaacgtc acggcctgat gtcgcggaac ctcgtggttc
      601 ggatcgcttt cgcagtcgtc gcgatccccc tcgcggtcct catcatctac gaaggcgatt
      661 ggatcctggc ggccatgctg gcggtgctcg gcgttctggg cacccgcgag ctctacgacc
      721 tcggccgcaa gcaggggatc gagcccatcg cgtggctcgg gtacctgtgc gccgccgcca
      781 tcccgctggg gatgctgtcg tcgatgatcg tcggttggcc ctacgcgctg gcgtgctggc
      841 tgatggcggt cctggtcgtc gccctggcca cgcggacgcc ggcgcagaag cccctgacgg
      901 ccgtcgccct caccgtgttg ggtcccttct acgcttcctg gatgctggcg ttcattctca
      961 agctccggag tggagccctg gggccctggc agggtacgtg gctggtgctc cttcccctcg
     1021 tcgccacctg gatctgcgac accgccgcga tgggcgccgg gtccgcaatc ggcggaccga
     1081 agcttgcccc ggtggtatcc ccgaagaaga cgtgggcggg cgcgatcggc ggcctgatcg
     1141 gcgcgatcgc cgcgaccgtc gcgatcggcc tttgggtgtt cccgcgtctg gggatggccg
     1201 tcggcgtcct gccgctcgtc gtgatcggcg tcgccatcgg cattctggcg caggtcgggg
     1261 acgtcgcgga gtcgctcttc aagcgtgaag ccggggtcaa ggactcgtcg tccctgattc
     1321 ccgggcatgg cggcgtcctc gaccgcctcg actcgctcta tttcgtactg cctgccgctg
     1381 ccggactctt ctggttcttc cgcattccat gaccaccagg tcggccggcg tcgcgatcct
     1441 cggatccacg ggctccatcg gacgttccac cctgcaagtc ctgtcgcggc agcgcgaccg
     1501 gtttcgcctc gtcgccctca ccgcgcacac caaccaggcc ctcctcgacg cgcaagccaa
     1561 agaatggaag ccggcgttcg cgaagtgcgt gaacggcaac gggcgtgagt gcctgattga
     1621 agcggccacc cgtcccgacg tcgacatcgt cgtgaacggc atcgtaggtg ccgcgggtct
     1681 cgaagcgacc ctcgccgcgc tgtccgcggg gaagcgcgtg gcgctcgcga acaaggagac
     1741 gctcgtgatg gccggcgagc tcgtcaccgc cgcggcgaag gcggggggcg gggagctcgt
     1801 gccggtggac tcggagcaca gcgcggtgct gcagtgtctc tcggggcgaa cccccgacga
     1861 cctccggcgc ctgattctta cggcatcggg tgggcccttc cgtacgtgga cgccgtcgca
     1921 ggtggcggcg gcgtcggtgg agcaggcgct ccagcatccc acctggaaga tgggccccaa
     1981 gatcacggtc gattcagcca ctctggtgaa caaggccctc gaggtgatcg aggcccattt
     2041 cctcttcggc ctggcgtacc catcggtcga ggtggtggtg catccgcagt cgatcatcca
     2101 tgcgttcgtg gagttccggg acggctcggt gctggcccag gtggggttcc ccacgatgga
     2161 gctgcccatt ctgtacgccc tgacccaccc ggagcgggtg gaggacagcg gcaccagggc
     2221 cttcgatccc ctcgcgacgg gcgacctgac cttccagccc gtaaacccgg cgctctttcc
     2281 ggcgtatgaa ttggggagag gcgcagggat ggcgggggga acggcccccg cggtgttcaa
     2341 cgcggccaac gaggtggcgg tggagctctt cctgagcggc acgatccggt tcggcagaat
     2401 cgcggcggtg atcgaaggca ccttgagcaa ggcgccagca ggggacgccg gtactctcga
     2461 cggcgtactg cacgccgacc gcgaggcccg gcggctcgcg cgggagattg catgctcctg
     2521 accgtcgcgg cgttcgtctt cgtcctgggc gtgctgatct tcgttcacga agcggggcac
     2581 ttcctggtcg ccaaggcgac gggcgtgcag gtcctccggt tctccctggg gttcggcaag
     2641 ccgctggtga aattcacctg gggtgagacc gaatacgtca tcgcgtacct cccgttcggc
     2701 ggctacgtga aaatggccgg actggaagaa gaagggatga tgggggaggt cgagggcggc
     2761 gcggcgacgg ttcccgtcga cccggagcgt gccttcgacc ggaagccatt ggtggtccgc
//
LOCUS       DASUBP010000003         4278 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_967617, whole genome shotgun sequence.
ACCESSION   DASUBP010000003 DASUBP010000000
VERSION     DASUBP010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4278)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4278)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4278
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_967617"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..781
                     /locus_tag="VFU75_00040"
     CDS             <1..781
                     /locus_tag="VFU75_00040"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5465244.1"
                     /translation="IAAATSLDASIPAWAHSGGTDSMLLRLKDPSTRARIRAELLAPP
                     NVTDRFMRAAGGPTGVIVLPFADSLSRFTGKRLSEIAALLHKDPIETLFDLVTEERAR
                     TGAIYFIMSEPDVDSALAKWWTSFDTDYGGVAPDGPFGHDGVHPRTYGTFAKILGHYV
                     REQHLLTLENAVRKMTSLPAQRVGLMDRGLLRPGLYADVTVFDPDSIADRATFEAPHQ
                     PSVGVTYLFVNGQMVLNHGQVTAARPGRGLRGPGYRPHTGT"
     gene            778..1143
                     /locus_tag="VFU75_00045"
     CDS             778..1143
                     /locus_tag="VFU75_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465245.1"
                     /translation="MTLGRLFLAWLPVAVWFTCATAIARRLALAPEGDGGASPLGSLL
                     RRTSGRSMAEALLLTLLASLWFDTLGTGAWWLPVALIGALVAIAPSSPRSRRTAALLF
                     LIDVLRYVGAGALLVWRLG"
     gene            1205..2254
                     /gene="selD"
                     /locus_tag="VFU75_00050"
     CDS             1205..2254
                     /gene="selD"
                     /locus_tag="VFU75_00050"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HEU5465246.1"
                     /translation="MTATAPVRLTSYSHGAGCACKLGPNDLAQVLSHIPVATDARVLV
                     DASNRDDAAVFKLTEDRALVATVDFFTPVVDDPYDFGRVAAANAFSDVYAMGGTPLLA
                     LNLVGWPREKLPFELLGEILRGGTEIAHEAGAFVLGGHSVDDPEPKYGMVAIGEVHPD
                     RIVTNAGAQPGDVLILTKPIGTGVLTTALKRDLLPAPELAPAVRSMTTLNAGAGRALR
                     AAPVHAATDVTGFGLLGHLNSVLRASRAAADLEAGSVPLLPRVREMALKGAIPSGTKR
                     NLASVAQAVTFDPTMAEVDRLILADAQTSGGLLISAPESSRDQLLAALKREGTLAAAV
                     IGRVRAGEPGSISVR"
     gene            2251..2487
                     /locus_tag="VFU75_00055"
     CDS             2251..2487
                     /locus_tag="VFU75_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465247.1"
                     /translation="MKAIPEYQGLTPDSLDALERAVREHRRVALRRRGTEYVVVAEEL
                     ETGDRDEVLKGRLPMTGEVLSFRLRELETFAVLP"
     gene            2501..3235
                     /locus_tag="VFU75_00060"
     CDS             2501..3235
                     /locus_tag="VFU75_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5465248.1"
                     /translation="MFTRLLIGLDGSPRADQALEQAVQLGHRFGSTLVVATVGAGEDS
                     GLLPRAAERVSAAGLKVETEARTGEPDVELADLAKNVDAVLVGRRGSGTREGALGPTV
                     ASLIRIAERCVVVCGAAPSPMNVCAVAYDGGDTSRRALDLAARFVSVDDGTVHVIHAA
                     KSKREGLKVVGAAEALLSLQGVRFVTHVEIGKPGEVVAALMHSTGADCLFAGAHVSRG
                     GDRPSQVVSHAEEILRHTDLPVIIQP"
     gene            3235..3822
                     /locus_tag="VFU75_00065"
     CDS             3235..3822
                     /locus_tag="VFU75_00065"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H"
                     /protein_id="HEU5465249.1"
                     /translation="MPDPDLPFIVVHADESCLGNGTTPPNPGGNAALIEARAGDSVAR
                     WDLYECAPETTNNRMALAGAIATLEWLHRQWQRAHIRYVSDSEYLVKGMNEWVSGWQA
                     RGWKRKTGPIENLPLWQKLVQAASGHAVEWHWVRGHDGHAKNEYADYLATRAAQKQER
                     SNGLVPSGFDTWLAQERARGRYTDYDPDRDLHVHT"
     gene            3809..>4278
                     /locus_tag="VFU75_00070"
     CDS             3809..>4278
                     /locus_tag="VFU75_00070"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deaminase"
                     /protein_id="HEU5465250.1"
                     /translation="MFTPKDGVEANTHYLNALTNDAPATTNRIPLYEVYMRMAEELAK
                     RSTCARLQVGTVVTNQALENVLAIGYNGNARGLPNRCDSTVPGSCGCIHSEMNALVKA
                     PGSVRDKVVFVSASPCVMCAKLMINSGVTHVFYRKAYRDPSGIEVLQQGGVVPVA"
BASE COUNT          671 a         1495 c         1439 g          673 t
ORIGIN      
        1 catcgccgcc gcgacgtcgc tcgatgcgtc gatccccgcg tgggcgcact ccgggggcac
       61 cgactcgatg ctgctccggc tgaaggaccc ctcgacccgc gcgcggatcc gcgccgagct
      121 cctcgcgcca ccgaacgtca ccgaccgctt catgcgggcg gcggggggcc ccaccggtgt
      181 gatcgtcctt cccttcgcgg actcgctctc gcgcttcacg gggaaacggc tctccgagat
      241 cgcggccctg ctgcacaagg accccatcga aacgctcttc gacctcgtga ccgaggagcg
      301 cgcgcgtacg ggcgcgatct acttcatcat gagcgagccg gacgtggatt cggcgctggc
      361 gaagtggtgg acgagcttcg acaccgatta cggcggcgtc gctcccgacg gaccgttcgg
      421 gcatgacggc gtgcatcccc ggacgtacgg gaccttcgcg aagatcctcg gccactacgt
      481 ccgcgagcag catctcctca cgctcgagaa cgccgtgcgg aaaatgacgt cgctccccgc
      541 gcagcgggtg ggactcatgg accgcggcct gctgcggccc ggcctgtacg ccgacgtcac
      601 cgtgttcgat cccgactcca tcgcggaccg cgcgaccttt gaggcgccgc accagccgtc
      661 cgtgggcgtc acgtacctct tcgtgaacgg gcagatggtg ctcaatcacg ggcaggtgac
      721 cgcggcccgc cccgggcgcg gacttcgcgg acccggctac cggccccaca cggggacatg
      781 accctcgggc ggctcttcct cgcctggctg cccgtcgcag tgtggttcac gtgcgccacc
      841 gcaatcgccc gccgtctggc gctcgctcca gaaggagacg gaggcgcctc tccgctcggc
      901 tccctcctgc gccggacgtc cgggcggtcc atggccgaag cgttgctcct gaccctgctc
      961 gcgtccctct ggttcgacac tttgggaaca ggcgcgtggt ggcttccggt cgcgctcatc
     1021 ggagcgctcg tcgccatcgc cccgtccagc ccccgctcac ggcgcaccgc tgcccttctc
     1081 tttctcatcg acgtcctgcg ctacgtcggt gccggggcac tgctcgtgtg gagactggga
     1141 tagagaggtc cggaaatcgt cgccccgccg cgaaggcgga agaccggagt atcttttccg
     1201 gactatgacc gcaacagctc ccgtccgcct tacgagctat tcccacggcg cggggtgcgc
     1261 ctgcaagctc ggccccaacg acctcgctca ggtcctcagc cacatcccgg tcgccaccga
     1321 cgcgcgcgtg ctcgtcgacg cgtcgaaccg cgacgacgcc gccgtcttca agctcaccga
     1381 agaccgcgcg ctcgtcgcca ccgtcgactt cttcacgccc gtggtcgacg acccgtacga
     1441 cttcggccgc gtcgccgccg cgaacgcctt ctccgacgtg tacgcgatgg gggggacgcc
     1501 tctcctggcg ctcaatctcg tcgggtggcc ccgcgagaag ctgcccttcg agctcctcgg
     1561 cgagatcctc cgaggcggca cggagatcgc gcatgaggcc ggcgccttcg tgctgggagg
     1621 ccactcggtc gacgatcccg aacccaagta cggcatggtc gcgatcggcg aagtgcatcc
     1681 cgaccggatc gtgaccaatg cgggcgccca gccgggggac gtcctgattc tcaccaaacc
     1741 gatcggcacg ggagttctca ccaccgcgtt gaagcgtgac ctcctccctg ctcccgagct
     1801 cgcgcccgcg gtgcggtcga tgacgacgct gaacgccggc gccgggcgcg ccctccgggc
     1861 cgctcccgta cacgccgcca ccgatgtcac cggcttcgga ctcctggggc acctgaacag
     1921 cgttctgcgc gcgagtcgcg ccgcggccga tctcgaagcc gggagcgtgc cgctgctccc
     1981 cagggtgcgc gagatggcct tgaagggggc cattccttcg gggaccaagc gaaacctcgc
     2041 gagcgtggcg caggcggtga ccttcgaccc cacgatggcc gaggtcgacc gcctcattct
     2101 cgcggacgcc cagacgtcgg gaggcttgtt gatctcggcc cccgaatcca gccgggacca
     2161 gctcctggcc gccctcaagc gcgagggaac gctcgcggcg gcggtgatcg gccgggtgcg
     2221 ggcgggcgaa ccgggctcca tttcggtgcg gtgaaggcga tcccggagta ccagggcctg
     2281 acccccgatt cgctcgatgc gctggagcgg gcggttcggg agcaccggcg agtagcgctt
     2341 cggcgccggg gtaccgaata tgtggtggtg gccgaggagc tcgagaccgg cgatcgcgac
     2401 gaagtcctca agggccgttt gccgatgacc ggtgaggtgc tgtccttccg cctgcgcgag
     2461 ctcgaaacct tcgcggtgct tccttaaaga ggcgacggcg gtgttcaccc ggctgttaat
     2521 tgggctcgat ggcagtcccc gcgccgacca ggcgctcgag caggcggtcc agctgggcca
     2581 ccggttcggc tccacgctcg tcgtggcgac ggtcggagcc ggcgaagact cggggctcct
     2641 gccccgcgcc gcggagcgcg tcagcgccgc gggactcaaa gtcgagaccg aggctcggac
     2701 cggtgaaccg gacgtcgagc tggccgacct cgcgaagaac gttgacgcgg tactggtcgg
     2761 cagacggggg agcggcaccc gtgagggagc gctgggcccg acggtggcct cgctcatccg
     2821 gatcgccgag cgatgcgtgg tggtgtgcgg ggccgcgccg tccccgatga acgtctgcgc
     2881 cgtggcttac gacggcggcg acaccagccg gcgagccctc gatctggcgg cccggttcgt
     2941 atcggtggac gacggaaccg tgcacgtgat ccacgccgcc aagtccaagc gggaagggct
     3001 caaggtggtc ggcgccgctg aggcccttct ttcattacag ggagtccggt tcgtgacgca
     3061 tgtggagatc ggcaagcccg gcgaggtggt cgcggccttg atgcacagca ccggagccga
     3121 ctgcctgttc gcgggagcgc acgtgagccg cgggggcgat cgccccagtc aggtcgtatc
     3181 gcacgccgaa gaaattctgc gccacaccga cctcccggtc atcatccaac cctgatgcct
     3241 gatcctgatc tgccgttcat cgtcgtccat gcggacgaga gttgcctggg aaacgggacg
     3301 acgccgccga accccggggg gaacgcggcg ctcatcgagg cccgggccgg agacagcgtg
     3361 gcccgctggg acctctacga atgtgccccg gagaccacca acaaccgcat ggccctggcc
     3421 ggcgcgatcg cgaccctcga atggctgcac cggcagtggc aacgcgcgca catccgctac
     3481 gtgtccgact ccgagtatct cgtcaaaggg atgaacgagt gggtgtcggg ctggcaggcc
     3541 cgcggctgga agcgcaagac gggccccatc gaaaaccttc cgctctggca gaaactggtg
     3601 caggccgctt cgggacacgc ggtcgagtgg cattgggttc gcggtcatga tggccacgcg
     3661 aagaacgaat acgccgatta tctcgccacc cgggccgcgc agaaacagga gcgctcgaac
     3721 ggcctggtgc cctccggatt cgacacctgg ctggcgcagg agcgcgcccg cggacgctac
     3781 accgactacg accccgaccg ggatcttcat gttcacacct aaggacggcg tcgaagcgaa
     3841 cacccactat ctcaacgcgc tgaccaacga cgctcccgcc acgaccaacc gtattccgct
     3901 ctacgaggtc tacatgcgga tggcggagga gctggcgaag cgctcgacgt gtgcgcgcct
     3961 tcaagtggga acggtcgtca cgaatcaggc gctcgagaac gtcctggcga tcgggtacaa
     4021 cgggaacgcg cggggactgc ccaatcgctg cgactccacg gtgccgggga gttgcggctg
     4081 tattcactcc gagatgaacg cgctggtgaa agcgccgggg agcgtgcggg acaaggtcgt
     4141 tttcgtcagc gcttcgccct gcgtgatgtg cgccaagctc atgatcaact cgggcgtgac
     4201 gcacgtgttc taccgcaagg catatcggga tccatccggc atcgaggtct tgcagcaggg
     4261 tggcgtcgtg ccggtggc
//
LOCUS       DASUBP010000004         5765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1199296, whole genome shotgun sequence.
ACCESSION   DASUBP010000004 DASUBP010000000
VERSION     DASUBP010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5765
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1199296"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..2051)
                     /locus_tag="VFU75_00075"
     CDS             complement(123..2051)
                     /locus_tag="VFU75_00075"
                     /inference="COORDINATES: protein motif:HMM:NF014731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HEU5465251.1"
                     /translation="MSASESQTGPSRPSAGPFAARHPRRHAAVLALTALGVVYGDIGT
                     SPLYALRECFFGPHSVPPTPENVVGILSLVFWALVLVVSVKYVAFILRADNRGEGGIL
                     ALMALLVPRNGEPKRRRWFIVGLGLFGAALLYGDGMITPAISVLSAVEGLNVATPVFE
                     PVVIPITIAVLVGLFLLQSKGTAGVGALFGPVMMVWFACIALLGIGGILHAPTVLGAV
                     NPAHALDFFSQNGLAGFLVLGAVVLVVTGCEALYADMGHFGRKPIRLTWYSLVLPALL
                     LNYFGQGALLLADPGAAHNPFYRLAPAWALYPLVALATAATVIASQAVISGAFSLTRQ
                     AVQLGYSPRLEIEHTSGVEIGQIYVPQVNWALMIATIGLVLGFGSSSRLAAAYGIAVT
                     GTMATTSVLFYFLARERWRWRPSLVAGLCTIFLGIDLSLFGASAVKLWQGGWFPLAVA
                     GGVFILMTTWKRGRELLAEKLSVVGVPVETFLTDLQSHRVIRVPGTAVFLTSNAEGIP
                     LALLHNLKHNKVLHEQVALLTIATSEAPHVPDRERLTVKPLGYGFFRLVARYGFMEDP
                     NVPALLDQARPLGLEARLLETTFFLGQETVVPGRRKGMPLWREKLFGFMSRNAHRATA
                     YFRIPPNRVIEVGAQIEL"
     gene            complement(2176..2349)
                     /locus_tag="VFU75_00080"
     CDS             complement(2176..2349)
                     /locus_tag="VFU75_00080"
                     /inference="COORDINATES: protein motif:HMM:NF016234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HEU5465252.1"
                     /translation="MPRAIERVLWVLRRIVGAPDYQAYLDHCRQAGHPPLLDEKAYIR
                     EFFDRKGGRTRCC"
     gene            complement(2333..4369)
                     /locus_tag="VFU75_00085"
     CDS             complement(2333..4369)
                     /locus_tag="VFU75_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009267 - cellular response to starvation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation CstA family protein"
                     /protein_id="HEU5465253.1"
                     /translation="MRRIASIVIWAAISALGALAFATLAWSRGETVSAAWLLIAAVCT
                     YAVGYRFYSKFIVTRVFTLDPTRATPAERLENGRDFVPTGKWVLFGHHFAAIAGAGPL
                     VGPVLAAQFGFLPGTLWLVIGVVLGGAVQDLTILCCSLRRDGKSLGQMAKEEISPAAG
                     MTAMLAVLFIMIILIAVLALIVVNALKASPWGFFTIACTIPIALLMGWWMRSWRPGHV
                     GEASLAGGVLLLLALVGGGWVSRHAGPAAAFTLTGTSLTALIVAYGFFASVLPVWMLL
                     LPRDYLSTFLKIATILGLAVAILILLPPLRMPAMTPFAKLGEGPVFAGKFFPFVFITI
                     ACGAISGFHALVSSGTTPKMLRREPDARMIGYGGMLMESFVGVMAIIAACTLHPGVYF
                     AMNTTPAALQQAAPVLQSAGFVVSPDSMAALAALVGEKTLVGRAGGAPALAVGMTQIF
                     SGVTHALGGQTLAALWYHFAIMFEALFILTTIDAGTRVGRFMLQELLGHVWPRMGETS
                     WYPSVILSSLVIVGGWGYFLYQGILDPLGGINSLWPLFGIANQMLAIVALAVATTIII
                     KMSRARYAWVTLVPLAWLVAVTMTAGFEKMFSSDPRLGFIAHAASLAGSVDPHAARLI
                     FNDRVNLVATFVFTAVVLLLLAISLREWILVLARRKPATVSEVPYVATSYAAGD"
     gene            4489..5295
                     /locus_tag="VFU75_00090"
     CDS             4489..5295
                     /locus_tag="VFU75_00090"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HEU5465254.1"
                     /translation="MPKPQERDDPVPAGDRFSGRVAMVTGGATGLGRAVCLDFAQRGA
                     AIAFNYFEMPGRDVTAQALLTETAIKAHGVPVFSSHCDVRSRNAVEVFVHEATERLGG
                     VHILINNAGVTHDGALWRLSTEAWQEVLDTNVTGAFNCIQSVSAHMRPQRYGKIVNVA
                     SHQAFRPGFGIANYTASKAALIGLTKSAAVDLGSSNINVNAVAPGFVKTELLAKLPRE
                     VLERAEHESVLGRVAEPEDVAHVITFLCSEAARHITGQTVVVDGGLTLAS"
     gene            5321..>5765
                     /locus_tag="VFU75_00095"
     CDS             5321..>5765
                     /locus_tag="VFU75_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465255.1"
                     /translation="MSNLLNRKYLKALLLLAASACVGHKSQTPIPEPTVPLPAGSLAS
                     RDVGVMPFTLVLAEDSLHWDAHFTDHPKTLATADSLLLGFLTSRVPEVAWKSPARLWR
                     SARGANDLPDDPHQLGVAILRQLPVGSYVPDPLRAQLRMLGAVAGG"
BASE COUNT         1078 a         1866 c         1992 g          829 t
ORIGIN      
        1 gcggtgggga cgggggtgtt ggttctcgcg tggtctgcag tggcactgag gcaagacgct
       61 aagtcactag gtcgctaagt cgccaggtac ttagtcgctt agcgacttgg cgtcttagcg
      121 tctcacagct cgatctgcgc ccccacctcg atcacccggt tcggcggaat cctgaaatag
      181 gcggtcgctc gatgggcgtt ccgcgacatg aatccgaaga gcttctcccg ccacagcggc
      241 atgcccttcc gtcttcccgg caccacggtt tcctgtccca ggaagaacgt cgtttcgagc
      301 aaccgcgctt cgaggcccag cggccgcgcc tggtcgagca gggccggcac gttcgggtcc
      361 tccatgaacc catagcgcgc caccagccgg aagaacccat atcccagcgg cttgaccgtc
      421 aaacgctcgc gatcgggcac gtgcggggcc tcactcgtcg cgatggtgag caacgcgacc
      481 tgctcatgga gcaccttgtt gtgcttcagg ttgtggagga gcgcgagggg gatgccctcg
      541 gcgttagacg tgaggaagac cgcggtgccc ggcacgcgga tcacccgatg agactggagg
      601 tccgtcagga aggtctccac cgggacgccg acgacggaga gtttctccgc caggagctcc
      661 cggccgcgct tccacgtggt catgagaatg aagaccccgc cggcgacggc gagcgggaac
      721 cagcccccct gccagagctt caccgcgctc gcgccgaaga gggagaggtc gataccgagg
      781 aagatggtgc agaggccggc caccagggag ggccgccagc gccagcgttc ccgggccagg
      841 aagtagaaga gcaccgacgt cgtggccatc gtgccggtga ccgcgattcc gtatgcggcg
      901 gcgaggcggg aagacgatcc gaagcccagc acgaggccga tcgtcgcgat catcaatgcc
      961 cagttcacct ggggcacgta gatctggccg atctcgacgc ccgaggtgtg ctcgatctcg
     1021 aggcgcgggg aatacccgag ttgcacggct tgccgcgtca gggagaaggc tcccgagatc
     1081 accgcctggg acgcgatgac ggtggccgcc gtcgccagcg ccacgagcgg gtacagagcc
     1141 caggcgggcg ccagccggta gaacgggttg tgggccgcgc cggggtcggc caggagcagg
     1201 gcgccctggc cgaagtaatt gaggagcagg gcggggagga ccagagagta ccaggtcagg
     1261 cgaatcggct tgcgtccgaa gtggcccatg tccgcgtaga gcgcctcaca tccggtcacc
     1321 accaggacga ccgcacccag gaccaggaac cccgccagtc cattctgcga aaagaagtcg
     1381 agggcgtggg cgggattgac ggcgccgagc acggtggggg cgtgcaggat cccgccgatc
     1441 ccgagcaggg ctatacatgc gaaccacacc atcatcaccg gcccgaacag tgcgccgact
     1501 ccggcggtcc ccttgctctg cagcagaaag agaccgacga gcaccgcgat ggtgatgggg
     1561 atgaccaccg gctcgaacac cggcgtcgcg acgttcaggc cctcgaccgc ggagagcacc
     1621 gagatcgcgg gcgtgatcat gccgtcgccg tacaggagtg ccgcaccgaa gagccccaat
     1681 cccacgatga accagcgtcg ccgcttcggc tcgccgttgc gcgggaccag cagcgccatc
     1741 agcgcgagga tccctccctc gccccggttg tccgcgcgga ggatgaacgc gacgtacttc
     1801 accgacacca cgagcaccaa cgcccagaac acgagcgaga gaatgccgac cacgttttcc
     1861 ggcgtcgggg gcacggagtg cggtccgaag aagcattcgc ggagtgcgta cagggggctg
     1921 gtgccgatgt cgccgtacac gacgccgagg gcggtcaggg cgagtacggc ggcgtggcgc
     1981 cgcgggtggc gcgcggcgaa cggcccagca gatggcctgg atggcccagt ttgagactcg
     2041 gaggcggaca tgacggggga gaacctaatc aacaacagcg ggtgccgttt cccaggctaa
     2101 agcctgggct cggctaagca ctgcgcttca agcgcagcga atcgccgagc acactcttga
     2161 gagtgtggga agcgatcagc agcatcgcgt ccgcccaccc ttccggtcga agaactcacg
     2221 gatataggcc ttttcatcga gcaggggtgg atgcccggcc tgccggcagt ggtcgaggta
     2281 tgcctggtag tcgggagcgc ccacgatccg gcgcagcacc cacaagacgc gctcaatcgc
     2341 ccgcggcata gctcgtcgcg acgtagggga cctcactgac ggtcgccggc ttccggcgag
     2401 ccagcaccag gatccattcc ctcagggaga tggcgagcag caggagcacg acggccgtga
     2461 acacgaaggt ggcgaccagg ttcacgcgat cgttgaagat cagccgtgcc gcatgcggat
     2521 cgaccgagcc ggcaagtgac gccgcgtgcg cgatgaaccc aagcctggga tccgacgaga
     2581 acatcttttc aaagcccgcc gtcatggtca cggccacgag ccacgcgagt ggaacgagcg
     2641 tgacccaggc gtagcgcgcg cggctcatct tgatgatgat cgtggtggcg acggcgagcg
     2701 ccacgatggc gagcatctgg ttggcgatgc cgaagagggg ccagagcgag ttgatgccgc
     2761 ccagggggtc gaggatcccc tgatagagga agtagcccca cccgccgacg atgaccagac
     2821 tggagagaat caccgaagga taccaggacg tctcccccat cctcggccac acgtgcccga
     2881 gaagctcctg cagcatgaag cgcccgacgc gggtgccggc gtcgatggtc gtgaggatga
     2941 acagcgcctc gaacatgatg gcgaagtggt accagagcgc cgcgagggtc tgtccgccca
     3001 gtgcgtgcgt gaccccggaa aagatctggg tcatcccgac ggcgagtgcc ggggcgcctc
     3061 cggcccgccc caccagcgtc ttctccccga ccagggcggc gagcgccgcc atcgagtcgg
     3121 gcgagacgac gaatcctgcg ctctgcagta ccggcgccgc ctgctgcagc gcggcggggg
     3181 tggtgttcat cgcgaagtag acgccggggt ggagggtgca ggcggcgatg atcgccatca
     3241 cacccacgaa cgattccatc agcattccgc cgtagccgat catccgggcg tccggttcgc
     3301 gccggagcat cttgggcgtc gtgcccgaag acaccagggc gtgaaatccg gagatggcgc
     3361 cgcaggcgat ggtgatgaac acgaacggga agaacttccc ggcgaacacc ggtccttccc
     3421 ccagcttcgc gaacggcgtc atcgccggca tgcgcagcgg agggagcagg atgaggatcg
     3481 cgacggcgag gccgaggatg gtggcgatct tcaggaacgt ggagaggtag tcgcggggga
     3541 gcagcagcat ccagaccggc aggaccgacg cgaagaaccc gtaggcgacg atcaacgcgg
     3601 tgagggaggt gcccgtcagc gtgaacgccg cggcgggccc ggcgtgccgg gacacccagc
     3661 cgccgcccac gagggccaga agcaggagca ctcctcccgc caatgatgcc tcacccacat
     3721 ggccgggccg ccaggagcgc atccaccagc ccatcaagag cgcgatcggg atggtgcagg
     3781 cgatggtgaa gaagccccac gggctcgcct tcaacgcgtt caccacgatc aacgcgagca
     3841 ctgcgatgag gatgatcatg atgaagagca ccgcgagcat cgcggtcatc ccggcggctg
     3901 gggagatctc ttccttggcc atctggccga gggacttccc gtcgcgccgg agggagcagc
     3961 agaggatggt gaggtcctgc accgcgccac ccagcacgac gccgatcacg agccacagcg
     4021 tgccgggcag gaacccgaac tgggcggcga gcaccggtcc caccaggggt ccggccccgg
     4081 cgatggcggc aaaatgatgc ccgaaaagca cccatttccc ggtcgggacg aagtcccgcc
     4141 cattctccag gcgctcggcc ggtgtcgcgc gcgtggggtc gagcgtgaag acccgggtga
     4201 cgatgaattt cgagtagaag cggtagccga cggcgtaggt gcagacggcg gcgatgagga
     4261 gccaggcggc gctgacggtt tcgcctcggg accaggcaag ggtggcgaac gcgagcgcgc
     4321 ccagggccga tatggcggcc cagatgacga tggaggcgat gcggcgcatc ccgctaaggt
     4381 acggctcgcc ttgccgaggt tcaaatggcg gcgtaggttc gcccgactgt ggtgaattac
     4441 ccctggtcat cgagcggccg tccgtcggga gcggggactg cgcccggtgt gccgaaaccc
     4501 caggaacgtg acgaccctgt gccggcgggc gaccgcttct cggggcgcgt cgcgatggtc
     4561 acgggcggcg ccaccggact cggccgggcg gtctgtctcg atttcgccca gcggggcgcc
     4621 gcgatcgcct tcaactactt cgaaatgccc gggcgcgacg tcacggccca ggccctcctt
     4681 accgagaccg ccatcaaggc gcacggcgtc ccggtgttct cgtcgcattg cgacgtccgc
     4741 agccgcaacg ccgtcgaggt cttcgtgcac gaggccaccg aacggctcgg cggcgtgcac
     4801 atcctgatca acaacgcggg tgtgactcac gacggcgcgc tctggcgact cagcaccgaa
     4861 gcgtggcagg aagtgctcga caccaacgtg acgggcgcgt tcaactgcat ccaatccgtg
     4921 agcgcccaca tgcgcccgca gcgctacggg aaaatcgtga acgtcgccag ccaccaggcg
     4981 ttccggcccg ggttcggcat cgcgaactac accgcgagca aggcggcgct gatcgggctc
     5041 accaagtccg cggcggtgga tctcgggtcg tccaacatca acgtcaacgc cgtcgcgccc
     5101 ggcttcgtaa agacggagct gctcgccaaa ttgccgcgcg aggtgctcga gcgggccgaa
     5161 catgagtcgg tcctggggcg ggtggcggag cccgaggacg tggcgcacgt cattaccttc
     5221 ctctgcagtg aagcggcacg gcacatcacc ggacaaaccg tcgtggtgga cgggggactt
     5281 actctggcgt cctgaccctc caaccgacga tattgggagg gtgagtaatc tcttgaaccg
     5341 caaatactta aaggctctgc tcttgcttgc ggcgtccgca tgcgtcggcc acaagtcgca
     5401 gacgccgatc cctgagccta cggtgccgct gccggcgggc tccctcgcga gccgcgacgt
     5461 cggcgtcatg ccattcacgc tggtgctggc cgaggactcc ctccactggg atgcgcattt
     5521 caccgatcat cccaagacgc tcgcgaccgc cgacagcctg ctgctcggtt tcctgacgtc
     5581 tcgagtgcct gaggtggcgt ggaaatctcc ggcccgactg tggcgcagtg cgcgcggcgc
     5641 gaacgacctc cccgatgatc cgcatcagct tggggtcgcg attttgcgcc agctcccggt
     5701 ggggtcgtac gtaccggatc ccctccgggc gcagctccga atgctggggg cggtggcggg
     5761 tggga
//
LOCUS       DASUBP010000005         4029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_245362, whole genome shotgun sequence.
ACCESSION   DASUBP010000005 DASUBP010000000
VERSION     DASUBP010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_245362"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..527)
                     /locus_tag="VFU75_00100"
     CDS             complement(<1..527)
                     /locus_tag="VFU75_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5465256.1"
                     /translation="MTDSIVQVRNVSKAYRRDANTVPVLQNITLDVPTGEFVALMGPS
                     GSGKTTLLNLIAGIDRPDSGSVMVAGTDVAQLTESQLAKWRATNIGFVFQFYNLIPVL
                     TAFENVELPLLLTSLSKSQRKAHVETALALVGLADRMDHSPRQLSGGQEQRVAIARAI
                     ATDPQILVADEPTGDL"
     gene            complement(524..1726)
                     /locus_tag="VFU75_00105"
     CDS             complement(524..1726)
                     /locus_tag="VFU75_00105"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5465257.1"
                     /translation="MDNKSADLSALRINRDAVPTPAGNSRRRLVIVGVPVVLLLAFAG
                     FLAVRSLGGGIQVHLTPAVRISPSQANVLASASGYVVAQRKAAVASKGTGRLVYLGVV
                     EGDKVKEGQVIARIEDADVKAALAQAHANLDLGNADLHDAKTSMERQATLLRQNLSTQ
                     ADFDAAQARYLRVKATIALDSAQVDAAQVAVENTIVRAPFDGTVLTKSADVGEVVSPL
                     TGGALAKASVVTLADLGSLQVEVDVSESNLAQVTIGEPCEIILDAYPDIRYAGSVAKI
                     VPTADRAKATVQVKVAFKHYDSKVLPEMSAKVHFLPRSNVAQTPEDTQSVIAVPEKAV
                     AQRNGQAVVYVVSDGRAVEVPVTPGRHFGSSVAILQGIAVGTAVVDSVDDRLHRGVKV
                     NVKSGTPQ"
     gene            2153..2716
                     /locus_tag="VFU75_00110"
     CDS             2153..2716
                     /locus_tag="VFU75_00110"
                     /inference="COORDINATES: protein motif:HMM:NF041523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-COAP-1 domain-containing protein"
                     /protein_id="HEU5465258.1"
                     /translation="MHVVKGMAPAAVAAALLVIAACEQPNAAGPQKAVAVARTPDGWV
                     GCDAGGKFTGGGRVDPSGVGKVTFGFNIHAEACDSGAIKGQLQVVYHATQTLIHSETM
                     EHFASYPDPTTGGSCGEWDGTARVKDVFAGSGWSDHHYFIQVCDNGEPGHGVDTFEFT
                     IDNAGDGTHNNVMTTVLTGGNIQAHKS"
     gene            2734..3351
                     /locus_tag="VFU75_00115"
     CDS             2734..3351
                     /locus_tag="VFU75_00115"
                     /inference="COORDINATES: protein motif:HMM:NF040603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor P family protein"
                     /protein_id="HEU5465259.1"
                     /translation="MNRIMQLSGVGLLLAAFPLAGQRSLTTATGSSMAVSVKSNGTTQ
                     QFAVAMLPAGGGQASDDAAMVSTPQLTSASTAAVTAGEVDTDGLGSQQSVATASNVNL
                     LNGLITADKVVGIASVTSNGNKSSGDYDGSVITNLTVNGTLVSGSDFTPAPNTKMTIP
                     GGYVVLNEQASAGHGKGTSVSVNMIHVYLSSGDEIVVGAASSSIQ"
     gene            complement(3429..>4029)
                     /locus_tag="VFU75_00120"
     CDS             complement(3429..>4029)
                     /locus_tag="VFU75_00120"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU5465260.1"
                     /translation="GDLIEVGRRVRLKRNVPLLKRLLDEGIDGHKFDFYVVSAAPEEV
                     VLSALHGIVAPDHIIGTQFEWDAGPDEIRNIVRATAGFGKVDAVESLREKLAIPREQV
                     VYVGDGQSDIPVMLHVNRGEGFTIAASEAREISHIAKRTVISDDALSVLVPILEDIVG
                     FDKPEIREYFASQGLVIQEWDRVRTDWLTIREGAEDAAA"
BASE COUNT          683 a         1304 c         1341 g          701 t
ORIGIN      
        1 aggtcgccgg tgggctcgtc cgcgaccagg atctgcgggt cggtcgcgat cgcgcgcgcg
       61 atggcgacgc gctgttcctg accgccggag agctggcggg gcgagtgatc catccggtcc
      121 gcgaggccga cgagcgccag cgcggtctcg acgtgcgcct tgcgctggga cttggagagc
      181 gacgtgagca ggaggggcag ctcgacgttc tcgaaggccg tgagcacggg aatcaggttg
      241 tagaactgga agacgaaccc gatgttggtc gcacgccact tggcgagctg cgactcggtg
      301 agctgggcga cgtcggtgcc cgccaccatc acggaccccg agtcggggcg gtcgataccg
      361 gcgatcaggt tgaggagcgt cgtcttgccg gagcccgacg gtcccatgag cgccacgaac
      421 tcgccggtgg ggacgtcgag cgtgatgttc tggagcacgg ggacggtgtt ggcgtcccgg
      481 cgataggcct tggagacgtt acggacctgc acgatggagt cggtcactgg ggggtccctg
      541 atttcacgtt gactttgacg ccgcggtgca gccggtcgtc gacggagtcc accacggcgg
      601 tacccaccgc gatgccctgc agaatcgcta ctgacgaacc gaagtgccgg cccggcgtga
      661 cggggacttc caccgcccgg ccgtccgaca cgacgtagac caccgcctgc ccgtttcgct
      721 gcgccaccgc tttttcaggc accgcgatca ccgattgcgt gtcctcgggg gtctgtgcga
      781 cgttggagcg cggcagaaag tggaccttgg cgctcatctc gggaagcacc ttcgagtcat
      841 agtgcttgaa cgccactttc acttgtacgg tcgccttagc gcgatcggcc gtcgggacga
      901 tcttcgccac cgacccggcg tatcggatgt cgggataggc gtcgaggatg atctcgcagg
      961 gctcgccgat ggtcacctgc gccagattcg actcggacac gtcgacctcg acctgcagtg
     1021 agcccaggtc cgcgagtgtc accaccgacg ccttggcgag ggccccaccc gtgagcggcg
     1081 acaccacttc cccgacgtcc gcgctcttgg tcagcacggt gccgtcgaag ggcgcgcgca
     1141 cgatggtgtt ctcgaccgcg acctgtgcgg cgtcgacctg cgcggagtcg agcgcgatgg
     1201 tcgccttgac gcgcaggtag cgggcctggg ccgcgtcaaa atccgcctgg gtggagaggt
     1261 tctgcctgag cagcgtggcc tggcgctcca tggacgtctt ggcgtcgtgc aggtcggcgt
     1321 tgcccaggtc caggttcgca tgggcctgcg cgagcgcggc tttgacgtcc gcgtcctcga
     1381 tccgcgcgat gacctgccct tccttcacct tgtctccctc gaccacgccg aggtagacga
     1441 ggcgtccggt acccttcgag gccaccgcgg ctttgcgttg agcgaccacg tagcctgacg
     1501 ccgacgcgag gacgttggcc tgggacgggg agattcggac cgcgggcgtc aggtgcacct
     1561 ggatcccgcc gccgaggctc cgtacggcca ggaaccccgc aaaggcgagg agcaggacga
     1621 ccggaacgcc gacgatgacc aggcggcgcc tgctgttgcc tgcaggcgtc ggcacggcat
     1681 cccgattgat ccgcagggcg gagagatcgg ctgatttgtt gtccatagcg ggcattaatg
     1741 ctaacacggc atttccctga cggggcaacg ggtccgatcc tggcaggtct cgatgcccct
     1801 gtctccagtc gcggccacgg cacgcgtcat ccccgggctg tcgcccgggg ttatgtacag
     1861 cgaaaccgcc attgggtggc ctttcatagc cccgggcgcg agcccggggt ttgtatatcg
     1921 acgttcagca gaggcggatt tcgacaggcc gaattcgcgt ctcgccggct acgccgccaa
     1981 caggcgccat ttggtgcttt ggccgcgatg aattcgcgca gcgaatcggg gacgccgcgc
     2041 ccgatttccc ccatgattcc tcgattttcg ctgggaccgt cgccacgcgc gtcggcattc
     2101 ctcttgccat ttctggcctc cgcactccaa cgcacacagg acaggaggtc ctatgcacgt
     2161 agtgaaggga atggcgccgg ctgccgttgc ggctgcgctc ctcgtgatcg cggcatgcga
     2221 gcagcccaat gccgccggtc ctcagaaggc agtggcggtc gcccggacgc cagatggctg
     2281 ggtgggatgc gatgccggtg ggaagttcac cggtggcggt cgcgtcgacc cgtcgggcgt
     2341 cggcaaggtg accttcgggt tcaacatcca cgccgaagcc tgcgattccg gcgccatcaa
     2401 gggccagctg caggtggtct atcacgcgac gcagactctc attcactcgg agacgatgga
     2461 gcacttcgcc tcgtacccgg accccaccac cggcggctcc tgcggcgagt gggatgggac
     2521 ggcgcgggtg aaggacgtgt tcgcggggtc ggggtggagt gatcatcact acttcatcca
     2581 ggtctgcgac aacggcgagc ccggacacgg ggtcgacacg ttcgagttca cgatcgacaa
     2641 cgccggggac gggacgcaca acaacgtcat gacgaccgtc ctgaccggtg gcaacatcca
     2701 ggcccacaag tcctaagccc ccaggagact cccatgaatc gcatcatgca actcagtggg
     2761 gtcggcctcc ttctggcggc cttccctctc gcaggacaga gatcgctcac gaccgcgacc
     2821 ggatccagca tggccgtgtc ggtcaagagc aacggcacga cacagcaatt cgcggtcgcc
     2881 atgttgccgg ccggcggcgg gcaggcatcc gatgacgccg ccatggtctc gactccccag
     2941 ctcacctcgg cctccacggc cgccgtgacc gcgggtgagg tcgataccga cggactcggc
     3001 tcgcagcaga gcgtcgcaac ggcctcgaac gtgaacctgc tcaatggact gatcacggcc
     3061 gacaaggtcg tgggcatcgc cagcgtgacc tcgaacggca acaagagctc gggggattac
     3121 gacgggtcgg tgatcacgaa tctgacggtg aacggcacgc tggtatcggg gagcgatttc
     3181 accccggcgc cgaacacgaa gatgaccatc cccggcggct acgtcgtgct caacgagcag
     3241 gcgtcggccg gccacggcaa ggggacgagc gtctcggtga acatgattca cgtctatctc
     3301 tcgtcgggag acgagatcgt cgtgggagcg gcgagcagtt cgattcaata acgccaacta
     3361 gacggtttga cggccagacg gttagggaag attctttcta gccgtctagc cgtctaaccg
     3421 tctagtagtt acgccgctgc atcctccgcc ccctcccgaa tcgtcagcca atcggtcctg
     3481 acccggtccc attcctgaat cacgagtccc tgggaagcga aatactcccg gatttcgggc
     3541 ttgtcgaagc ccacgatgtc ctcgaggatc gggacgagca ccgacagggc gtcgtcggag
     3601 atgacggtgc gcttggcgat gtgggaaatc tcgcgcgctt cggacgcggc gatggtgaac
     3661 ccctcgccgc ggttcacgtg cagcatcacc gggatgtccg actgcccgtc tcccacgtag
     3721 accacctgtt cgcgcggaat ggcgagcttc tcccgcaacg actcgacggc gtcgaccttc
     3781 ccgaacccgg ccgtggctcg aacgatgttg cggatctcat cgggcccggc atcccactcg
     3841 aactgggtac cgatgatgtg gtcgggggcg acgatgccgt ggagggcgga gagcacgact
     3901 tcttccggcg ccgcggagac cacgtagaag tcgaacttgt ggccgtcgat cccctcgtcc
     3961 agcagacgct tcaacagggg gacatttctc ttgagtctca ctcgccggcc cacctcgatc
     4021 aggtcgccg
//
LOCUS       DASUBP010000006        48048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1240139, whole genome shotgun sequence.
ACCESSION   DASUBP010000006 DASUBP010000000
VERSION     DASUBP010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 48048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48048
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1240139"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="VFU75_00125"
     CDS             complement(<1..314)
                     /locus_tag="VFU75_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465261.1"
                     /translation="MRRSMLAICALLAVTTAGCDQLNKVKGLFSKKAPPAATPQHPAT
                     PPAQQTPAPPPTGTAKPAQTSRPGQTAQAPGGPVTPIQDKPYSSPDTGTVAPGMSESQ
                     VYA"
     gene            complement(256..972)
                     /gene="purQ"
                     /locus_tag="VFU75_00130"
     CDS             complement(256..972)
                     /gene="purQ"
                     /locus_tag="VFU75_00130"
                     /inference="COORDINATES: protein motif:HMM:NF002957.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEU5465262.1"
                     /translation="MRRVAVVVFPGTNSEVETVDAARDAGSDPYYVWHKDTDLKSADV
                     VILPGGFSYGDYLRSGAIARFSPIMEAVAAHARAGGPVLGLCNGFQILCEAGLLPGGL
                     VRNDRLRYVSRPVRLRVEAVDTPFTNAYRKGEEITIPIAHGDGRYVADAPTLAALEAE
                     GQVVWRYAGENPNGSTNGIAGVCNAARNVVGVMPHPERLNAPVLGGQDGARVFFSLEA
                     RDATQHAGDLRAVGGHHRGM"
     gene            complement(972..1226)
                     /gene="purS"
                     /locus_tag="VFU75_00135"
     CDS             complement(972..1226)
                     /gene="purS"
                     /locus_tag="VFU75_00135"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEU5465263.1"
                     /translation="MASFRVEIRVMPRGGILDPQGQAVTHALHALGFADVNGARVGKH
                     LVLTVDAASSSAAEARVKAMCDRLLANPVTEDYQLAVEPA"
     gene            complement(1226..2089)
                     /gene="pssA"
                     /locus_tag="VFU75_00140"
     CDS             complement(1226..2089)
                     /gene="pssA"
                     /locus_tag="VFU75_00140"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HEU5465264.1"
                     /translation="MAGPRLRPPNIRRVVIIVPSLFTLFNLFFGFWSMVLAQRGDFYR
                     AGWFIVFAGILDTLDGRVARLSNTGTRFGAELDSLVDVVSFGVAPAFLMYRVEFADAG
                     PVAWVFCFFYVMAAALRLARFNITQAGRAKTAFVGLPSPAAGMTLATFYPFTQTGLYD
                     ASVHFIPWHTVLSFLMIGLAILMVSNVRYATVPRAGVRTVRGILAFAVIIAILVFGVW
                     KHDVFFFPLGVTYVTYGVVRAAVLGLFVTADEDEEAETAGPIVIGDTDRGSGELHRHR
                     HPHVPRGPEGA"
     gene            complement(2089..2739)
                     /locus_tag="VFU75_00145"
     CDS             complement(2089..2739)
                     /locus_tag="VFU75_00145"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HEU5465265.1"
                     /translation="MRMAPEGWPFILPGWALVALGVWAATTSSPWWWVGTLVWLPFAV
                     WLLVFFRDPLRSGPRGEKTVISPADGTIVSVAQVDEPAYLKAPAVRVSIFMSVFNVHV
                     NRYPVTGTVEYRYYNPGKFLHAARDKASLDNEQSSIGVRGPRGPVLVRQIAGSIARRI
                     VTDSKVGDPAVQAERMGMIRFGSRVDVFLPASANAKIAVKVGDKVHAAATVLAEYP"
     gene            complement(2739..3659)
                     /locus_tag="VFU75_00150"
     CDS             complement(2739..3659)
                     /locus_tag="VFU75_00150"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEU5465266.1"
                     /translation="MTTALRESTLPLPLLRRGKVREMYEVDADTLLMVASDRVSAFDV
                     VMNEAVPHKGAVLTQMSAFWLKKLDKVIPNHMITADAGEIIKKVPALTAHRAVIAGRA
                     MLVKRTTPVSFECVVRGYLSGSAWAEYQKSGTLAGEPLASGLIESDRLDPPIFSPATK
                     AETGHDENVTFTHVVDALGRVMGERLKRASLSLYDAGRAYAAERGIIIADTKFEFGTT
                     TDGTLLLIDEVLTPDSSRFWPADRYTPGKSQPSFDKQPLRDYLAGIKKQGKWNGEAPP
                     PALPKDVVQATSERYREAYLLVTGHALETA"
     gene            complement(3656..5089)
                     /gene="purB"
                     /locus_tag="VFU75_00155"
     CDS             complement(3656..5089)
                     /gene="purB"
                     /locus_tag="VFU75_00155"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEU5465267.1"
                     /translation="MTYSSPLGTRYASPAMQSLWSDQRRYGLWRRLWLALMESERELG
                     LDIPDQAIQQLRAHLDDVDLARAAEHERRLRHDVMAHIHALGDQAPAARPYLHLGATS
                     AFVTDNADLLLMREGIQLLLGRVAAVLVALGKLSRRTGRIPCLAYTHFQPAQLTTVGK
                     RVTLWMQDFILDAEELLHRLETFRFRGVKGTTGTQASFLELFAGDHDKVRKLDQLVAQ
                     KLGFSQVFPVTGQTYTRKLDAEVLAALSGIAASAAKFATDLRLLQHEGELLEPFESEQ
                     VGSSAMAYQRNPMRAERMTSLARFVIELEGNAWHTAATQWLERTLDDSANRRLVIPEA
                     FLAADAILVLATNVAAGIEVREAVIARRVADQMPFLATETLLMRGVKAGGDRQTLHEV
                     IRSHSQTVAKAMAEAGTPNDLLERLDSDPAFRALDLSARTGDLDPAKYVGRAPQQVDE
                     FLDDVLPPILHAVQRAAPTATPAEVTV"
     gene            complement(5086..5754)
                     /gene="fsa"
                     /locus_tag="VFU75_00160"
     CDS             complement(5086..5754)
                     /gene="fsa"
                     /locus_tag="VFU75_00160"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEU5465268.1"
                     /translation="MKFFLDTANLKDITWAAQAGLIDGITTNPSLLAKFAGDVDPRDV
                     LKEICSLVEGPVSAEVIAIEADGMVREGESLMKIADNIVVKIPMTEAGMVAVRRLSKQ
                     GIKTNVTLCFSSVQCLIAAKAGASYVSPFIGRLDDVGQEGMDVIRETRVIFDNYQYAT
                     EILAASIRHPRHVVEAAMVGADVATLPPDILKKLLQHPLTDRGLEQFLADWGKLTSRA
                     KATV"
     gene            complement(5751..6536)
                     /gene="truA"
                     /locus_tag="VFU75_00165"
     CDS             complement(5751..6536)
                     /gene="truA"
                     /locus_tag="VFU75_00165"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEU5465269.1"
                     /translation="MTSRPFLAVVQYDGGQFVGWQRQPASRTVQGEMEAVLQRIIDRR
                     TVVTGAGRTDTGVHALGQGAGFLAPERWAGDVTGLHRALNALLPKDIWVERVVEMRPG
                     FHPRKSATARRYQYVIGTDAAAESPFRRRFEWALHRALDADALATCARTLIGEHDFRG
                     FAAAGQVKPHYRCRVALAEWAPRADGAGVTFTIEADRFLHHMVRFLVGTMVDIALGRR
                     PASDFPRLLAATDNRAASPPAPPQGLYLVCVRYPADLYAGASP"
     gene            6645..7697
                     /gene="trpD"
                     /locus_tag="VFU75_00170"
     CDS             6645..7697
                     /gene="trpD"
                     /locus_tag="VFU75_00170"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEU5465270.1"
                     /translation="MPDTSASSPLTQAIAILARGEPLSEALATQVFEVVMRGDATAAQ
                     MGAILLGLRAKGETAAEVAGVVRALRATMVRVTADGAHVVDTCGTGGGTINTFNISTV
                     AAFVAVGAGATVAKHGNRSYTSLCGSADVLEALGVRIPPGPDEAERALARARIAFLFA
                     PHFHPAMRHLVPVRKDLGVTTVMNLVGPLANPAGVRRQVVGVADASRAPLVAEALALL
                     GTEHSLVVHGRAGLDEISPDGLTDVWEIRGGRTSHWVLDPRSLPVAHVDLRDLRGGQP
                     SENARRAEQLLDGTGRDEAGKTAVLLNAAAAVYVAGLARDLAEGLERARAALESGAAR
                     EALRKLRDGDEVSTSE"
     gene            complement(7681..8307)
                     /gene="lexA"
                     /locus_tag="VFU75_00175"
     CDS             complement(7681..8307)
                     /gene="lexA"
                     /locus_tag="VFU75_00175"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEU5465271.1"
                     /translation="MPLTRRQHEILDYLREHIDDKGYAPSFEEIAERFEFQSLATVHE
                     HLTNLERKGYIRRTHNESRAIEIVPPKGQTGATELPLLGLVAAGAPIEALTEGETFSV
                     PDELLPRRGRSYVLRVRGSSMIDEHIKDGDYVVVQERNQADNGETVVALLNGIAGGGA
                     GGATVKKYYREPGGWIRLQPANAELSPIRVNERDVMVQGVVVGVIRKY"
     gene            8366..9157
                     /locus_tag="VFU75_00180"
     CDS             8366..9157
                     /locus_tag="VFU75_00180"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEU5465272.1"
                     /translation="MPKASIDAILALTRTRVQGLRGRRVELERRAAGAPNPPVFGPGV
                     VARAVGIIAEVKRRSPSAGDIRADLDPVRHALAYQAGGAVTISVLTEEAHFGGSLDDL
                     EQVTKAVSIPVLRKDFIVDELQLLEARAARASLVLLIVRALTDVELRRLAAEATGLGL
                     ATLVEAHDREELDRAFAVSPTAVGINNRDLSTFVTDLRVAETLVPKVPKGILAVAESG
                     IEERAAVERLAAAGADLVLVGEAVARAQDPALAVRGLSGVPRRPR"
     gene            9154..10353
                     /gene="trpB"
                     /locus_tag="VFU75_00185"
     CDS             9154..10353
                     /gene="trpB"
                     /locus_tag="VFU75_00185"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEU5465273.1"
                     /translation="MTDAQVEGRFGPYGGRYVPETLIAALEELARTYDDARQDKAFWD
                     ELGLLLRDFVGRPTPITDLSRISEVVGNGVRVACKREDLNHTGAHKINNTLGQVLLAL
                     RMGKRRIIAETGAGQHGVATATVCAKFGLECVVYMGEEDTRRQRLNVYRMGLLGATVV
                     PVTSGTRTLKDATNEALRDWVTNVDTTHYIIGSVVGPDPFPRMVRDFQAIIGQEARAQ
                     VLERYGRLPHTVVACVGGGSNAMGIFEGFRRDQAVRLVGVEAAGEGLSGERHAASLSK
                     GRPGVFHGSLSYLLQDSDGQVQPAHSVSAGLDYPGVGPEHAYLKDTGRVLYHAVTDQE
                     ALDAFKDMSRLEGIVPALESAHAVAWVLGHRGARVWGPDDVVLINLSGRGDKDVAWVA
                     EVTGASL"
     gene            10365..12029
                     /locus_tag="VFU75_00190"
     CDS             10365..12029
                     /locus_tag="VFU75_00190"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU5465274.1"
                     /translation="MDELSLPPSQITELLSLITKGVRATQLYLPNNPVYQRAVDNVRG
                     GFRQVFQSVEELPLQITESELKWEDEVVYSNENKSESFAWLLFKDGLRSLTFKQGVEA
                     DELPKFLRILHQARTLQADAADDMLTLLWEQDFQYIAHTFRELLSENAVPIEAGEPIP
                     EVTKSDVVQYVQEEAPPRPKGMVSIEDFDTTLYFLDEKEIAYIHSEVDREYSQDLRGN
                     VIAMLFDLLELQTYGTVRAELISILENLIPYLLGAGDFRSVAYILRETRVILQRAREL
                     IAEHKQSLEALPHRLSQPEALSQLLQSLDEATVHPTEDELSGLFQELRPEALVTLMGW
                     VPRLSNERVKSLVVASAEQLAQANAGEVLKALASEDGDTQLEMVKLAGRLKLAGAPDG
                     MGRLLEIGERPLKLAIIEALTAIGSPSAMRVMERAIEDPDRDVRIAAVKFLSAKGHRN
                     AFKQIDAAVNGSKLKDSDLTEKMAFFEAYGAMAGAAGVTPLSRMLLSRPTLLGRGEGP
                     ETRACAAMALGKIRTPESRNVLERAKDDKEALVRNAVSRALRELGA"
     gene            12026..13510
                     /locus_tag="VFU75_00195"
     CDS             12026..13510
                     /locus_tag="VFU75_00195"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEU5465275.1"
                     /translation="MTDPRTPSPAPRRATISAESQASDAFLRHAGREFLVVLYTAFRS
                     LKLYPIENAQVQKALDELAASTKALLNVENELEVRLQGEFIFVNSTRLRLDLDNYASF
                     SHILGVFRQSGIGAVRVLPGVDRKQLQIFVSLLLAYAARDADANKVHELSQKLDESGV
                     NQIDVEPPLDTDDDAEDVEKQKEAAKRTYARSVAVTKEVINSIRMGRTANVKKVKRAV
                     QAIVDQVLNNESSLMGLTTLRDYDEYTFTHSVNVCIFSVALGRKLGLSKVQLYDVGMS
                     ALFHDVGKSRVPLEVLNKEGGLTDDEWRIMQAHPWLGVLTLFGLRGYGEIPYRGMIAA
                     YEHHMKNDLTGYPKSIRPRTLSIYSKIVAVADGFDAATSRRAYQTTPIQPDQVLKEMW
                     ENPRRGYDPTVVKAFINLIGIYPVGTCVILDTYEVAVVHAANADTASVHRPIVRIVTS
                     PDGGLLHPGFLADLALQEDGAYPRSIIKVTDPLKYGIKVQDYFV"
     gene            13503..14300
                     /gene="trpA"
                     /locus_tag="VFU75_00200"
     CDS             13503..14300
                     /gene="trpA"
                     /locus_tag="VFU75_00200"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEU5465276.1"
                     /translation="MSDSRSRLAERWRMAKADGRAALITYLTAGYPTPKDFVAGLRAG
                     AAAGADIVEVGVPFSDPLADGPVIQRATHAALGQGVGVRRTLDLIAEAQLAVPVIVMT
                     YLNPVLAYGVDRFITDAAQAGVAGVLLTDLPAGADPDLEARVAAGPLAFVRLIAPTTP
                     QQRIERAVQGASGFLYLISRLGVTGARDRIPVDLPEYVKRVRAATDLPLAVGFGIGTA
                     AQARDAAKLADGVVVGSALVDVLGREGASGVGRLVRELAAGVRGQAA"
     gene            14297..16384
                     /locus_tag="VFU75_00205"
     CDS             14297..16384
                     /locus_tag="VFU75_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEU5465277.1"
                     /translation="MSVETLVRLYARGLQVAGGLLLLAVLAADPRWLHQVGTLLVIIV
                     ACVALRARPIALSKYSYLTQTALVALAGALLVGVPVTCLGLVLGVVIADWGLQRKAIP
                     VALVNAGREVIALVASYGVYAEIRHLSDNVTPAFSTASIPPLFFFALAYFAISRVLFY
                     FTLILRAKLERDEKLLIVRYELIGYGATLFATTTVVGVMREWPPVSWLFVAAVLGAVG
                     LLLKRMLEEAISAEELNKIHAMEAVITSNVSLEDSFRRIEGLAHRLVDWGDFRIFRHA
                     DAGMQLAYRSDLGRPDRGAGAPDGGVAVLRNEAVEKGEPIVVDDVGRDQRLVNPPDHV
                     RSLVIVPLKFGDQVIGTLELEHHKRRAYRDTDVVTISTFAAQLATAIHITDLRRPLAD
                     TVERMTSELATLARTAVALREVARGVASATHAIRSGVVNEESEIARGLEATKSLSEVS
                     VRVSSDGAAAAKSSSEASETASRNRQRIQDAIERLVALKTFVGESSGKVQELGVVSRR
                     ITGFIASIRELADMTNLLALNAAIEAARAGKHGKGFAVVAEEIRHLAEQSGAAAAEAG
                     GLVDDVHRQVGEVVEQMRRGQVNVGGVEELSSGALDALDRIVATTADATEHARRIAGA
                     AAEQNQAFGRLTERMTAVAGIAWKNREEADDVATRATQAADGLTDLERATKELELVAT
                     NLRELTRGFANLA"
     gene            16403..16828
                     /gene="folK"
                     /locus_tag="VFU75_00210"
     CDS             16403..16828
                     /gene="folK"
                     /locus_tag="VFU75_00210"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEU5465278.1"
                     /translation="MARGERVFVALGSNLGDRAQWLAEARERLAALPETRMVRASSVE
                     ETAPLGDVPQGPYLNQMVELDTGLEPEALLDACLGIEAALGRERTVRWGPRQIDLDIV
                     RYGARAVHTGTLTVPHPELPNRPFWRRELAELQDLSEDS"
     gene            16825..17637
                     /gene="panB"
                     /locus_tag="VFU75_00215"
     CDS             16825..17637
                     /gene="panB"
                     /locus_tag="VFU75_00215"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009245056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HEU5465279.1"
                     /translation="MTDALKQVTTYELLKMKERQERIVMLTCYDALFARLLDPSVEIL
                     LVGDSVNQVLGGQDTTLSATLDQMIYHTKTVRRGSSHALVICDLPFLTYQVSPTEAMR
                     NAGRVMQETLCHGVKLEGGRSMAPTVKALVDIGIPVMGHLGLTPQSVNALGGYRVQGR
                     DDVTAQRLKDDAKALEDAGAFAVVLELVPTPLSAEISKSLSIPTIGIGAGPSCDGQVL
                     VLHDMLGLNDRFSAKFVKKYATLADDVRKAAEAFKAEVRSGRYPDADHSFAK"
     gene            17655..18527
                     /gene="panC"
                     /locus_tag="VFU75_00220"
     CDS             17655..18527
                     /gene="panC"
                     /locus_tag="VFU75_00220"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HEU5465280.1"
                     /translation="MQDLTGLPEMRAWTRAQRGRGKRIAFVPTMGALHEGHLRLIDRA
                     REVADLVVMSVFVNPLQFGPTEDFSRYPRDLDRDRVLAAERGVDCLFAPDRAAMYPAE
                     PLVRMDPGPLALRFEGAVRPGHFAGVLTVVAKLFHLVEPDVAVFGRKDFQQAMLIRRM
                     ARDLDFPLEIVIAPTVRELDGLALSSRNAYLNSDQRRAALGLSRTLRTVEQLWRGGDA
                     DPVSIRTQGLLVLSAAGVVPDYLALVDEDLHDVDRVTAAAIAIVAGKVGNTRLLDNVI
                     LGEGVSGDPSIRKP"
     gene            18524..19021
                     /locus_tag="VFU75_00225"
     CDS             18524..19021
                     /locus_tag="VFU75_00225"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU5465281.1"
                     /translation="MTLPGWAVAGPERRAHVERVAALIDQWAVVMRVPERERGRWAKA
                     AWLHDALRDAPAPDELAHGPASADRAAREGESDRGVLDAVRYHSLGFAGWDDVGKMLY
                     LADYLEPGRRFEREARAALAARVPLERDAVLLEVTRRRIEWTVRSGWPLSPETVEFWN
                     SLVAP"
     gene            19048..19434
                     /locus_tag="VFU75_00230"
     CDS             19048..19434
                     /locus_tag="VFU75_00230"
                     /inference="COORDINATES: protein motif:HMM:NF024791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HEU5465282.1"
                     /translation="MTALAVLGGCRNASPRMSGDVVPGDGAAAPRVMVAVLNASGRPG
                     LARLGTRVLRDAGIDVLSFGNSSDPKHPLDSTRILVRRGPAAVGERIRRALKVGKVVM
                     DPDSTLLLDASVLLGADFSPRLPLHP"
     gene            complement(19372..20436)
                     /gene="rlmN"
                     /locus_tag="VFU75_00235"
     CDS             complement(19372..20436)
                     /gene="rlmN"
                     /locus_tag="VFU75_00235"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: protein motif:HMM:TIGR00048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEU5465283.1"
                     /translation="MTDLLSLTPDGARTALGEWLATRGEPAYRLRQILPRLWERPVAR
                     WADATDLPAPLREALDQDFPLGRLTVAAHQVSSDGTEKFLWTLADGQAIESVLIPEGK
                     RRTLCISSQVGCALGCVFCATGTMGWSRNLSPAEIAGQVREIKLKNPALSPTNVVFMG
                     MGEPLLNWENVDVALTILNHPEGLGIGARHITLSTVGILPKLALFAKRPEQFRLAVSL
                     HAPTPALRKELMPVEKKYSLPELLEALKGFERRVTIEYVLIAGKNDSLEAAEALAKVA
                     SPLAALVNLLPLHPGGAPGLTPSPRTHMIAFERRLKERGIEAVLRRSRGLDIAAACGQ
                     LRVERETGGKVRAQEHAGVE"
     gene            complement(20433..21338)
                     /locus_tag="VFU75_00240"
     CDS             complement(20433..21338)
                     /locus_tag="VFU75_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465284.1"
                     /translation="MNATWRFGLAGVLALGTPRVTQAQGDPVGAELWRVVGTTVAVPF
                     ALAQGTAAAFWNPAQTVSVASVGGDIVQTPEAISATGILAVIRTGSGRLGSWGLILGQ
                     VDIGDITHTTFTPDPQGTIQVYTRAVGVTWAKAWGSTTVGATLGTHDSRLDQTDLQHW
                     TGDVGVTQHFGDVLTLAAATHFFYRFATSDPSQDLYAGVEWRAAHGPLWTGSGEVALR
                     LRYGATLAHGVGTDHAIGVGVDVGAPFAIDGMLVRESAFGNTAWRGVAGLRVHIGHYG
                     IAFARDAGVSGLGSSYRVGLEARFK"
     gene            21384..21869
                     /locus_tag="VFU75_00245"
     CDS             21384..21869
                     /locus_tag="VFU75_00245"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU5465285.1"
                     /translation="MRLSELLSPSRIRVPLKSHDKDGILGELVELLLPGAGGSGGPGG
                     GERQEVLEAILERERQFPTGIGFGVAVPHGKTPALSSLGVVAGTATEPVPYETVDGEP
                     VRLFFLMAGPETAAGTHVKAISRIARLVRHEAVRSRLLAARDPDEFYRVLCEAEEGGG
                     A"
     gene            21866..23641
                     /gene="aspS"
                     /locus_tag="VFU75_00250"
     CDS             21866..23641
                     /gene="aspS"
                     /locus_tag="VFU75_00250"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF001750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEU5465286.1"
                     /translation="MTDLEKTAWRSTYCGALRAEHVGRKVTLGGWVHRRRDLGGIVFL
                     DLRDRAGLVQVAVGPAWAGADVVTRAGGLGAETVVVVEGEVVARPANMKNAELATGDV
                     EVHATSLRVVGPAETPAIPVARGKGEELASEEARLTHRHLDLRRPEMQANLILRHRLL
                     QRARRTMSELGFLEIETPILTKPTPEGARDYLVPSRVHPGEFYALPQSPQIYKQLLMV
                     SGFDRYFQIARCFRDEDLRFDRQPEFTQIDIEASFVGPEDVQRFVEAVLVACWAEAGQ
                     SVPSPFPTLTWKDAMARYGTDKPDLRYDIRLGDWTREFASWTDLPLAGALASDPKNRL
                     EGFVVPGGAALSRKEIDALSEIAKKAIGKTVVWVKRQGDQISGSVKLTAERAAALGVA
                     DGAMAIMLFGAPSEIRPALGTLRAEAIKRLKLAPSATHAFCWVVDFPLFEPDPETGKP
                     VFAHHPFTSPHPDDVAKLDAEPFACRALHYDAVYNGNELGSGSIRITDPAVQRTVFRH
                     LGLTPAEIDARFGFLLKALAAGAPPHGGFAVGFDRVTMLLAQATSLRDVIAFPKTTAA
                     RALFEGAPSPANPADIAALHLEVTR"
     gene            23638..24591
                     /locus_tag="VFU75_00255"
     CDS             23638..24591
                     /locus_tag="VFU75_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEU5465287.1"
                     /translation="MSDDVIRRLSVEGVDPLTLAGVNDANLIELSKRLGVRVSLRGDT
                     LTLQGKPEAVDHAVPITQALVDLARVGDPVDVHDVERIVLEDGAPAAAGEGGEYKIIL
                     PGLRRVIQAKTAGQREYLQAIQQKEIVVGIGPAGTGKTYLAVAAAVDALARHRVRRII
                     LARPAVEAGENLGFLPGDLQQKVDPYLRPLYDALEDMMPRDRVEKALETRVIEIAPLA
                     YMRGRTLSDAFVILDEAQNATGMQMKMFLTRLGVNSRAVITGDKTQIDLANREDSGLL
                     QVERILPGIDGIAFCYLTDTDVVRHRLVREIIKAYAEDSQG"
     gene            24569..26035
                     /locus_tag="VFU75_00260"
     CDS             24569..26035
                     /locus_tag="VFU75_00260"
                     /inference="COORDINATES: protein motif:HMM:NF019318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEU5465288.1"
                     /translation="MRKTVKADPVPREERIRYHLIRWWWVIGLAVLAETAFSFGAGGA
                     VDVETMLGVMLENALILCVFWVLLFYYRRETYRDNRQVAMIGGLFAFMIIIAAIIGAL
                     APQHPELVPLPLFAMILTAQFNGRVSMIGAMILAVLVDIQPVFHNGPALFVCLAGGIT
                     AALSVRTLRRRSHLYLAVLITAIGYLDAAIALGLVGHWPIAQIGWMTLLGAANALVSA
                     SLTILLLPLIESVTTVTTDLTLLELSDPGRPLLRRLSLEAPGTYAHSVAMANLVEAAC
                     DAIGANGLLGRVGCYYHDVGKLGNPGFFVENQNRGTSNPHDRITAQQSAKIIRQHVVD
                     GVRLAQEARLPEIITAFIPEHHGTSEINYFLDKAKKSRDGVVNPADYRYPGPRPRSIE
                     TAIAMLADSVEASLRVLDDLTPGRIEGVIDHLVKNKLAAGQLDEAPITLQQIDDVKAE
                     FVRVLSGMYHNRIDYPEDRGGISAGWQSSQPSAPAAGA"
     gene            26055..26426
                     /gene="ybeY"
                     /locus_tag="VFU75_00265"
     CDS             26055..26426
                     /gene="ybeY"
                     /locus_tag="VFU75_00265"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEU5465289.1"
                     /translation="MRRVLAWEGAPGARVSVTLLPPARMKALNRRTFGHRGLTDVISF
                     PLPQPDGTLHGDVYVCPAAARAWVKDAGPGALREEMVRLVVHGTLHVLGYDHPSGPGR
                     TRSRMWRTQERYVRRLVGAGK"
     gene            26423..27589
                     /locus_tag="VFU75_00270"
     CDS             26423..27589
                     /locus_tag="VFU75_00270"
                     /inference="COORDINATES: protein motif:HMM:NF015436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEU5465290.1"
                     /translation="MTGGGGALLLVEALIALGAALWAGLLSLGEEAAVGETAHTMADA
                     PPVARVPLHRGLHVARLALLVLAAVAGASALSWWTRGPMGGLVSVAVATGFLFVVGDA
                     LPRSLASLAPELAAAILPLAHRSLAVFDFLLAFLGWADRGLHGLVHLPRPASPVLGGA
                     QRDMLLGVFMMADTTVDEVMTPRLDLVGIDVSAPYDELLRVFKESGHTRLPVFDGSPD
                     AVLGVVFAKDLVPIALGLKPRPDEWRELVRSVAYVPEAKVLDAQLHDFQRGRAHIAIV
                     VDEFGGTSGIVTLEDILEEIVGEIRDDPATEPPSIEQDGDRYWVHGGVGLDQLSQTLG
                     ADFHNAEVSTVGGLIYSVLGRVPRSGEELHLDGFRVVVERVDRRRVTRVYFERE"
     gene            27586..28791
                     /locus_tag="VFU75_00275"
     CDS             27586..28791
                     /locus_tag="VFU75_00275"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEU5465291.1"
                     /translation="MTAGLLLLTLALLVAATSATIGVAAASVSQAELGRWVSLRLRGS
                     GSTSALVENPGRVLATANALTTLGVLAAGTAVPALFLELAPTFLGIVTFAVVVPVFVV
                     ASYVVPRVLGRRWAERIVSRTLPAINWLSSALGPFLPRRDPSTRHALAAMLTGAGSDA
                     LAGVDELEVVSGVLAFADRPVRDVMTPRTAIVALPGGTAAGEAAHVFLQSGYSRYPVL
                     GKSLDDIVGVTYSKDLLWLDPNAPIPVRPILTVLSGRPAADLLLEMQRGGARLAVVVD
                     EFGGTAGVVTFEDLLTTLMRDLFPLEPQAPEKETPILEIEGGAPVERLLERFGHPREA
                     RGAQTVGGYLTQLLGRIPKAGERIAVEGLEFDVVAASTVRIERVLVRSGPVRVTALDS
                     RADATPGSV"
     gene            28797..29858
                     /gene="meaB"
                     /locus_tag="VFU75_00280"
     CDS             28797..29858
                     /gene="meaB"
                     /locus_tag="VFU75_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HEU5465292.1"
                     /translation="MEMGTESLLEGLERRDPAALARAISLVESRRPGYEGVLSHVHGR
                     VGAKGARRIGITGPPGAGKSTLTERLVQHFRGAGLKVAVVAVDPTSPFTGGALLGDRI
                     RMESVSLDPDVFIRSMATRGSLGGLAMATEEVADVLDAFGFERILIETVGVGQTELDI
                     ARTAESTVLVLVPESGDAIQTLKAGVMEIADIYAVNKSDRPGADRLRQEIEVMLGIRR
                     GNAFRHVSAHHKPDPKGPDARARKGVQQSAAAAEWEPPVLATVASQGEGIGDVVAALD
                     RHRTYLESSGKLLERRRRRLTERTRSLVERHLAEWLWDESAAGEVLERRLDAMVEGDR
                     NPYEVAAEILDQVKGGVTP"
     gene            29855..31417
                     /locus_tag="VFU75_00285"
     CDS             29855..31417
                     /locus_tag="VFU75_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU5465293.1"
                     /translation="MSIERKPVYTPDDLAEFDPAKQLGTAGSFPFTRGIHDTMYRGRL
                     WTMRQFAGFGTAEDTNQRYKFLLEHGQTGLSVAFDFPTLMGYDSDHPRSEGEVGKCGV
                     AISSLADMETLFDGIPLDQVSTSMTINGPAAMLFCFYVAAAERQGVSIEKLRGTVQND
                     ILKEYMAQHAWVYPAEPALKIIVDMFEWCAEHTPQWNTISISGYHIREAGATAAQELA
                     FTLANGFTYVERGIARGLDVDTFASRLSFFWDIHNDFFEEIAKLRAARRIWARHMKDR
                     YRAKNPRSWMMRFHSQTAGVTLTAQQPLNNIVRVAYQALAAVLGGTQSLHTNSLDETL
                     ALPTEQAVQVALRTQQILAHETGVPEVIDPLGGSYYIESLTDQLEREAEALFAEIQSL
                     GGVVPGIETGWFQRQIARSAAEQQRNVEDGKSVIVGVNEFVASEDHPVEILKVSGEAE
                     DSQKAKMAALRARRDDALVKSRLEALRVAAQTDQNVIGPMLDCARAYCTLFEIRHALE
                     TVYGAYREPVFF"
     gene            31420..31839
                     /locus_tag="VFU75_00290"
     CDS             31420..31839
                     /locus_tag="VFU75_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HEU5465294.1"
                     /translation="MANSKKRPIRVLVAKPGLDGHDRGAKVVAAALRDAGMEVIYTGL
                     HQTPEMIARAAVQEDVDVVGLSILSGAHLTLFPRVKQLLKDAGRHDVLLTGGGIIPPE
                     DVSRLEAEGVGKLFGPGTPTSELIKYIEEWAATHVAA"
     gene            31840..33408
                     /locus_tag="VFU75_00295"
     CDS             31840..33408
                     /locus_tag="VFU75_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HEU5465295.1"
                     /translation="MSRLQALTAEYRELKSRIEAGGGPDKVAKQHDQGKLTARERVSA
                     LLDQGGPWLEIGLLMAFDQYDGQAPAAGVVTGLGRVGGRPVVVVANDATVKAGSWWPE
                     TIKKILRAQEIAMRQRIPIIYLVDSAGVNLPYQGGVFPGQYGAARIFYYNSIMRHYLR
                     VPQISAVMGPCIAGGAYLPALSDVIFMVEGTSFMGLGGPNLVKGATGQTVDAETLGGA
                     STHTSVSAVAHYKAKNDQECLTRIREYVGRLPRATPAVLNGSAPKKNGKALYDALPAD
                     HRMSYDTHLVLEGLLDGGAFDPFQPDVAPEILCGHGRIAGRPVAVIANARQLIKGKKG
                     EPPKIGGIIYTQSAEKTAYFIENADRERIPLLFVQDVSGFMVGTDAEHSGIIRAGAHF
                     VEAMATATVPKIVLTLNHASGAGYYAMAGQGFDPDFILSWPTGRMGVMEGESAVQAVF
                     GAGAAKPENAGAVEAMRADYEHQLDAKYAAARGYVDGIITPEETRDMLAFLFETAAEY
                     AGPHLGTFSLPESR"
     gene            33408..36248
                     /locus_tag="VFU75_00300"
     CDS             33408..36248
                     /locus_tag="VFU75_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU5465296.1"
                     /translation="MVRRPVGWAAAGLTLSLACAGAKPTSDVTPRSAPAPSTTAPVES
                     PSPPVPAPVDVMPGLRPVMSPAAAYLAGMLSLRSAGVDRFAAAHPTYDGRGVIIGILD
                     TGVDPGVPGLITTSTGAPKVIEERDFSGEGQVALTPFTPEGDHVSIGGHTFGGAGRIG
                     RMAVLSTWYGGFLREINLGPAPGGDLNGDGDNTDVFPVVVVKATDGWVAFLDSNLNGS
                     FEDEMPLHDYREGRQTISLGSKPVTLAANFAEVNGTPVVDIVGDNLAHGTHVAGIAAG
                     HNIYNVAGFDGVAPGAQILALKIANNARGGISVNGSMIRAMRYAAAYAAARGLPLVLS
                     LSFGVGDEHDGPVVIDSLVNEFLKTHPDVVLAVSAGNDGPGLSTLSYPGSADLALAVG
                     SLLPGSFAKPTVPGDVPTPDVMGDWSSRGGNAGKPDIVTPGVAYSTVPRWDTGHEIKV
                     GTSMSAPYAAGLVARLLSAMSQEQRHVTAADLIASLRATAAPLAGWTSLDDGAGTPRL
                     EAAYRWLVAGHQGARYQVFADQGSSGGFRRDGLAAGDTVAHFRIVHVDGFRPAHVDLK
                     DDASWMSVPSVVTSDPLGINLSVPMDPRRLTAPGLYVGTVTASVPDDTLAGATFTVKN
                     TVVVPYDLTRGPLSDLHRRIVPSQVRRYFLSVPTPGATLSIAVRGSGPSDAATVHLYE
                     PPGRPFRDGDKAETGPDAAPWAAFRVRGEDLVPGVYELDVTAPPLTTTNVDVHAELSS
                     ASLEVTGSGIEISNATSSSISGTLSVRVIGAERDIQVAGRGAPPESIAVQVPRWAARA
                     EVDVEMPRAQWGRFTDFGVTVYDTAGQHLNDAPLNYAIGRQSFEVPAGIAGAPALIEL
                     FPALALVDRAGPWQATVRVRFLMDSASATGGARRPIAVVAGGRSAVETNDLSPPALPE
                     GFHPLLDARVTVPGSSDAERRVVMGATGGVAR"
     gene            36245..37615
                     /locus_tag="VFU75_00305"
     CDS             36245..37615
                     /locus_tag="VFU75_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyclic terpene utilization AtuA family protein"
                     /protein_id="HEU5465297.1"
                     /translation="MTKSVVRVAGAQGFWGDWLEAPYRQLVGGPVDYLMLDYLAEVTM
                     SIMQKQKSRDPSLGYAKDFIPLMERILPELVKRKVKVTSNAGGVNPRACAEAVAQAAR
                     KLGLAGKLTIGVVTGDDLLPRLDQLLAAGHPLANLDTGRPLTDVRAKVQSANAYLGAA
                     PVVEALRQGADIVITGRVTDTGLTLGPLMYEFGWRADDWDRLAAGTVAGHTIECGAQS
                     SGGNDLADWGRVPDLANVGYPIVEARPDGTFAIIKHPRTGGAITVAGVTEQLVYEMGD
                     PHSYITPDCVADFTTIQLKQKGKDRVEFSGIRGKPATDKFKVSCAYFYGYKAVGTLVY
                     AWPDAYRKAQAADRVLRQRLKDLGLSYETVLTEFVGVDATHGPLAGAPSPDLAEVQLR
                     VGVRASDKAPVERFTREIAPLILNGPPSVTGFAGGRPKPEEIVAYWPALLDKREVTAC
                     VDLVRV"
     gene            37612..37950
                     /locus_tag="VFU75_00310"
     CDS             37612..37950
                     /locus_tag="VFU75_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465298.1"
                     /translation="MKKKSKPKLTPLVAIAHARSGDKGDTANVGLIARQAKHYPLLVK
                     EVTAARVRRHFKGVITGPVERYELPNLKALNFLLHGALDGGGTISLKTDAQGKVFSTA
                     LLRMRIKVPR"
     gene            37947..38705
                     /locus_tag="VFU75_00315"
     CDS             37947..38705
                     /locus_tag="VFU75_00315"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEU5465299.1"
                     /translation="MSDRVLVNLTEGILTGTLNRPDKKNAIDTAMMEALAELLDQADL
                     DAGVRLLVIRGAGKDFCAGMDLNELLASADQTVEQNRAAALRFGNLFIKMRALPKPVV
                     AVVQGRALAGGCGLATACDLVLASETAQFGYPEVQRGFVPALVMNMLKRAVGEKVAFD
                     LAATGHLLNAAEAHAVGLVSRVYDDSDFEEQVGDLLRTIAESSGTALAFTKRQFYQLD
                     GVSFDAGIRLSADVNAASRATADFRASIARFLKK"
     gene            38702..39190
                     /locus_tag="VFU75_00320"
     CDS             38702..39190
                     /locus_tag="VFU75_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465300.1"
                     /translation="MTERPRGFTQPKAAADLVKSIVQQKVEDAKQVKAEAEKARKKNK
                     SKVPLLLGLTPVLLGLTAWNVLSSGPPESKLTAADQLASTKFRIYIAAEAIEGYRAAH
                     GVYPANLTQVGVDWQGLHYATSDTTWSIVAQADTVTVTYRHGDPITPFATAYQSLQRR
                     KR"
     gene            39187..39642
                     /locus_tag="VFU75_00325"
     CDS             39187..39642
                     /locus_tag="VFU75_00325"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU5465301.1"
                     /translation="MKGGDRRGFTLIELLVTLIIVGILANIAIPLMRVVKVRANAAKV
                     LSDFDVIRGAAYDVYASHDSFPPSGTFGQVPPWFVQSLPKGFQFQYQDVQYRWSLLGT
                     PQGNPLSPGQPLLGLEIQSSNTELMNAILAGYHGALAFGGPNDFTLVIE"
     gene            39617..39823
                     /locus_tag="VFU75_00330"
     CDS             39617..39823
                     /locus_tag="VFU75_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465302.1"
                     /translation="MTSPSSSSDAPPQAGSGGPGRLFHIKAALGGAGLLLGLVGMSLH
                     RTSLVWGAVGCLGVAFVLRFVKPR"
     gene            39820..41208
                     /locus_tag="VFU75_00335"
     CDS             39820..41208
                     /locus_tag="VFU75_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_223146966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CUAEP/CCAEP-tail radical SAM protein"
                     /protein_id="HEU5465303.1"
                     /translation="MIFDPGAVLLVSCYELGHQPLATSQAAAFLQRAGLQPTQLDLAQ
                     EVLTPAAARGAEVIVFSVPMHTALRVGVEAATLAKRLNPGVRIGFFGHYAWLNRQYLL
                     DGVADWVLAGEGEEALVERLSTGRIQTDAPVLRRLDFPVPYREGLPSLDHYVMMEHGE
                     RRVAAGYVEGSRGCLHLCRHCPIPPVYGGRFFAIPVETVLADIRALVAAGAGHITFGD
                     PDFLNGPGHARKLAQALHREFPEVTFDFTAKVEHLLKHRALLPELVQAGALFVVSAVE
                     SLSDTVLGHLEKGHTRSDVFEALRLTREVGLALRPSLVAFTPWTTLDDYVELVEFVGD
                     QDLTRHIDPIQLTIRLLIPPGSGLLERDAVRPFLGPLEPARLGYSWTHPDPRMDALYT
                     VVTKVMEQGNNEGADPEVIVERIRAQAVTIRDGRPASPRPTTPSVTPVPRLTEAWFCC
                     AEPAPEQLVTLR"
     gene            41274..41909
                     /locus_tag="VFU75_00340"
     CDS             41274..41909
                     /locus_tag="VFU75_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465304.1"
                     /translation="MDAYTFSLALGGIGLLVMGVSGLGHRMHPGGHAAHGHGHHGMVK
                     AGGKAQAAHHGTQQAHGINVGIARVSRAFLTILSPRVAFSLVLGFGATGFLLKGIFGG
                     AILVAFAAVGGVAFEGILVRPLFNFLSKFASDPALTLDATLGDEVQVVTGFDTQGQGL
                     VSVELNGQIVQLLGTLTPEDRSGPRVRAGDTLFVRDVDSARHRVVVGRTPD"
     gene            41938..43233
                     /locus_tag="VFU75_00345"
     CDS             41938..43233
                     /locus_tag="VFU75_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465305.1"
                     /translation="MQNWMVIGGVVVGVLVVIPLIVSTFLRDVAAGEIRLVSWIAGGT
                     VTYRGPGKSKEIPLLTTGTTLSSKVINVDLDITDQTADLDTSGTPRPIKVRVLASAIV
                     SVGDSDVLIKTAANRFFSKSDGDQVSTLTDLLSSSGRRAINLLTHDQLFSAKTAGRPE
                     GANLPAPIATGTDLVAAGAVEDDDDPLSVIIRKACSRELTDLGLIFNSLNIKVVQSEV
                     ADARRRQSAAEAQANADIVSAQQGRRAKEAQLEAERAISDKQRELEQTKASNAALIAQ
                     ATAKMQEALGVQREAELDATQIAQAKADAEKMRLGAAAAADAEAIRITKIAEAQSESI
                     KKVNEAIKAGGESYFRYRQIEMLPQIAPVIADALAQAKLVTISGGEGGGAAGTATNNV
                     ASVIQTVLAAQLVTKGILSPESDGGNNTPAAPVTTAKNR"
     gene            43239..43676
                     /locus_tag="VFU75_00350"
     CDS             43239..43676
                     /locus_tag="VFU75_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HEU5465306.1"
                     /translation="MSNLHVPMYDPNLVQPMREELVRLGFEELRTPDRVDEVMAKAAD
                     TTLVVVNSVCGCASGRARPATAMALRGARKPSRLLTVFAGQDAEATARAREYFAPYPP
                     SSPQIALFKDGKLVFMLERKNIEGRTPSDIAADLSGAFDRFCA"
     gene            43676..44056
                     /locus_tag="VFU75_00355"
     CDS             43676..44056
                     /locus_tag="VFU75_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HEU5465307.1"
                     /translation="MDDGAKKLIVVGDRVLVLPQEGEERTNVGLYLPATALEGRQVQG
                     GRIVATGPGSPMAEPASMDDEPWKIRSSDVKYLPMQARVSDYALFFRKAAVEITFDGK
                     TYLVVPQAAILVLVRDGVPLDDRA"
     gene            44119..45285
                     /locus_tag="VFU75_00360"
     CDS             44119..45285
                     /locus_tag="VFU75_00360"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecQ family ATP-dependent DNA helicase"
                     /protein_id="HEU5465308.1"
                     /translation="MTSFPGGLERARRLLAAHWGYGGFRPNQVRVLAPLLGGRDVLAI
                     LPTGAGKSLCFQIPALMSSGLTVVVSPLVSLMQDQVGALRRRGVAAAYINSTQTLEHR
                     HAVLDAVRAGLVRLLYCAPERLPSLTRRLAAWTLRPALLAVDEAHCITAWGHEFRPAY
                     QLLGKYRYLMGNPPTIAVTGSATTLARAEIARVLRLEGVVEVVGSFDRPNLRFTTELV
                     QDDRGRFAKIVERVRRATAATIVYAPTRRLTELITRALLLRSLRAAPYHAGLTAAVRR
                     STLKAFLSDQVGTVVATTAFGMGIDKPDVRSVLHWGPAHTLDGYYQEAGRAGRDGHSA
                     ECRVFWRDGDLRWPGIAPAMKVYLQRRACRRRLLLQYFGERLERCAGCDYCDIR"
     gene            complement(45267..46427)
                     /locus_tag="VFU75_00365"
     CDS             complement(45267..46427)
                     /locus_tag="VFU75_00365"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU5465309.1"
                     /translation="MKLRVPLPALLLAIALASGCHDSATTVITRAGGPVLIPVDSGYD
                     FSVFVAAPPGDTSRLFVVERGGRILIRKHGVRQDSAFLNLTALTGGALEYGVYSIAFH
                     PHYATNRYVYVFYVDNAGAAQVSRFTADSTFDHADPATEHSILNVPQAADAVLYGGLV
                     TFGPDGKFYIAIGDGDANVDTAQTSRSQDSMAYQGKLLRVDVDAGDPYGIPADNPYVA
                     RSGWLHEIWQLGLRNPWRWSFDRQTGDVWIGDVGQNLWEEINYLPGGARPGNNFGWPF
                     MEGDVCYRTGCDTAGLVLPLFKYPHNPACAVTGGYVYRGKASPDLSGVYLYGDYCAGW
                     VRGLKMVAGKPVDAFTPLEAPLINDNVVSFGEDASGEVYVVMASGRIYRMSQ"
     gene            46462..47682
                     /locus_tag="VFU75_00370"
     CDS             46462..47682
                     /locus_tag="VFU75_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5465310.1"
                     /translation="MHDELYFSEQHLQVREMVRDFARNVVRPSARAYDLDSKFPWDNV
                     KQMADLGLFGIPWPEELGGSGMDYLSYMIVIHEMAKVDASHAITISAHTTLGTSPIVD
                     FGTPEQKKKYVPLLATGKVLGGFGLTEPGAGSDAGGTKTVAVEKGDHYVLNGSKIFIT
                     HAGVGEIFVATARTDPSAKKTHGITAFIVTKDTTDLAEATRVGIGHEASLAKIPGFSA
                     GKKEDKLGWRASDTRELVFQDAIVPKENVLGPVNNGFRQFLHTLDGGRIGIAALSLGI
                     AEGAFEECLAYTSTRKQFGKPIASFQGVHFALADMATEIECAKHLVFHAAWLKQHGHP
                     FKREAAMAKLYASELAMRATTKAVQLHGGYGYTTEYPVERMMRDAKICEIGEGTSEIQ
                     RIVIARQFLGDLID"
     gene            complement(47761..>48048)
                     /locus_tag="VFU75_00375"
     CDS             complement(47761..>48048)
                     /locus_tag="VFU75_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEU5465311.1"
                     /translation="LSTLAALQAPVIMMSQNRQAEKDRLQAKQDYEINLMAEIEIRDL
                     HDKLDSLRYKQWHELWHLQQRQLELLEHLHKELSHPEEKTVEPGPYKPPEL"
BASE COUNT         7700 a        15825 c        16695 g         7828 t
ORIGIN      
        1 gcgtagacct gcgactccga catgcccgga gccacggtgc ccgtgtcggg tgacgaatac
       61 ggcttgtcct ggatgggcgt caccggacca cccggcgctt gcgccgtctg gccaggacgt
      121 gaggtctgtg caggcttcgc cgtgcccgtc gggggcgggg caggagtctg ttgtgccggc
      181 ggcgtggccg gatgctgcgg ggtggcggcg gggggcgcct tcttggagaa cagccccttc
      241 accttgttga gctgatcaca tcccgcggtg gtgaccgcca acagcgcgca gatcgccagc
      301 atgctgcgtc gcatcgcgag cctccaagga gaaaaagaca cgggctccat cctggccgcc
      361 cagcaccggc gcgttcagtc gctccggatg cggcatcact ccgaccacgt tccgcgcggc
      421 gttgcacacg ccggcgatgc cattggtcga accattggga ttctctccgg cataccgcca
      481 caccacctgg ccctcggctt ccagggcggc cagggtgggg gcatcagcaa catagcgccc
      541 gtcgccgtga gcgatgggga tggtgatctc ctcccctttc cggtaggcgt tggtaaaggg
      601 ggtatccacc gcctccaccc gcaggcggac ggggcgggag acgtaccgga gacggtcatt
      661 ccggaccagg ccgccgggca ggaggccggc ctcgcacagg atctggaagc cgttgcaaag
      721 ccccaacacg ggcccgccgg cccgggcgtg agccgctacc gcttccatga tcggagagaa
      781 ccgcgcgatg gctccggagc ggaggtagtc gccgtagctg aagcctccgg gaaggatcac
      841 gacgtcggca ctcttcagat cggtgtcctt gtgccagacg tagtaggggt cgctcccggc
      901 gtcccgggcc gcgtccacgg tctcgacctc ggagttggtg ccggggaaga cgaccaccgc
      961 gacccgccgc atcaggccgg ctccacggcg agctggtagt cctcggtcac cggattcgcg
     1021 agcaagcggt cgcacatcgc cttgactcga gcctcggccg cactcgagct cgccgcgtcc
     1081 accgtcaaca ccaggtgttt gcccacccgg gcgccattca catcggcaaa gccgagggca
     1141 tggagcgcgt gggtcaccgc ctgtccctgc gggtcgagga tgccgccccg gggcatgacg
     1201 cggatctcaa cgcggaagga ggccatcagg cgccctccgg gccacgcggg acgtgcgggt
     1261 ggcggtgccg gtgcagctca ccggagccgc ggtcggtgtc gccgatgacg atggggccgg
     1321 cggtctccgc ttcctcatct tcatccgccg tcacgaacag gccgaggacg gcggcgcgga
     1381 cgactccgta ggtcacgtag gtcaccccga gcgggaagaa gaagacgtcg tgcttccaga
     1441 cgccgaagac cagtatcgcg atgatgacgg cgaacgccag gatgccgcgg accgtgcgga
     1501 ctcccgcgcg cggcaccgtc gcgtatcgga cgttcgagac catcagaatg gcgagcccga
     1561 tcatcaggaa gctcaggacg gtgtgccacg gaatgaagtg gaccgaggcg tcgtagaggc
     1621 cggtctgggt gaacggatag aacgtcgcca gggtcatgcc ggccgcgggg gagggcagtc
     1681 cgacgaacgc cgtcttggcg cggcccgcct gcgtgatgtt gaagcgcgcg agccggaggg
     1741 ccgccgccat cacgtagaag aagcagaaga cccaggcgac cggccccgca tcggcgaact
     1801 cgacgcgata catcaggaac gcgggagcga caccgaagga caccacgtcc accagcgagt
     1861 ccagctccgc gccgaagcgg gtgcccgtat tcgagagccg cgcgacgcgc ccgtcgagcg
     1921 tgtcgaggat gcccgcgaag acgatgaacc agccggcccg gtagaagtcg ccacgctggg
     1981 cgagcaccat cgaccagaag ccgaagaaca gattgaagag cgtgaacaag ctcggcacga
     2041 tgatgacgac gcgccggatg ttcgggggac ggaggcgggg acccgccatc agggatactc
     2101 cgccagcacg gtcgccgccg cgtgcacctt gtctccgacc ttcaccgcga tcttcgcgtt
     2161 ggcggaggcc ggcaggaaca cgtcgacgcg cgagccgaac cggatcatgc ccatgcgttc
     2221 cgcctggacc gccggatcac cgaccttgga atcggtgacg atgcggcgcg cgatcgagcc
     2281 ggcgatttgc cggacgagca ccggtccgcg tgggccgcgc accccgatcg acgactgctc
     2341 attgtcgagt gacgccttgt cgcgcgcggc atggaggaac ttgcccgggt tgtagtagcg
     2401 gtattcgacg gtgccggtca ccggataccg gttcacgtgc acgttgaaca cgctcatgaa
     2461 gatcgagacg cggaccgcgg gtgccttgag gtacgcgggc tcgtccacct gcgcgacgct
     2521 gacgatcgtg ccgtcggcgg gcgagatgac cgtcttctct ccccttgggc ctgagcggag
     2581 cggatcgcgg aaaaagacca ggagccagac cgcgaacggc agccagacga gcgtccccac
     2641 ccaccaccag ggactgcttg tggtcgcggc ccacacgccg agcgccacga gggcccagcc
     2701 ggggagaatg aagggccagc cttccggcgc catgcgcatc aggcggtctc caatgcgtgt
     2761 ccggtcacca gcagatacgc ctcgcgatac cgctcactgg tggcctggac cacgtccttg
     2821 gggagtgccg ggggcggcgc ttcgccgttc cacttgccct gcttcttgat gccggcgagg
     2881 tagtcgcgca ggggctgttt gtcgaaggac ggctggctct tgcccggggt gtagcgatcg
     2941 gcgggccaga agcgcgagct gtccggcgtc aggacctcgt cgatgagcag cagggtcccg
     3001 tcggtcgtgg tcccgaactc gaatttcgtg tcggcgatga tgatcccgcg ctcggcggcg
     3061 taggcgcggc ccgcgtcgta gagcgacagg gaggcgcgtt tcagccgctc gcccatgacg
     3121 cggccgaggg cgtcgaccac atgggtgaac gtgacgtttt cgtcgtgacc cgtctcggcc
     3181 ttggtggccg gggagaagat cggcggatcg agccggtcac tctcgatgag tcctgacgcg
     3241 agcggctcac ccgccagggt ccccgatttc tggtactcgg cccaggcgga gcccgacagg
     3301 tagccgcgga cgacgcattc gaaggaaacg ggcgtcgtgc gcttcaccag catcgcgcgc
     3361 ccggcgatca cggcgcggtg agcggtgagc gcgggcacct tcttgatgat ctcgccggcg
     3421 tccgcggtga tcatgtgatt cgggatcacc ttgtcgagct tcttgagcca gaacgccgac
     3481 atctgggtga gcaccgcgcc cttgtgcggc acggcctcgt tcatgaccac gtcgaaggcg
     3541 ctgacccggt cgctcgccac catcaggagc gtgtccgcgt ccacttcgta catctcgcgg
     3601 accttgccgc ggcgcaggag gggcaggggg agcgtgcttt cgcgtaacgc cgtcgtcata
     3661 ccgtgacctc ggccggggtc gccgtggggg ccgcccgctg gaccgcgtga aggatgggag
     3721 ggagaacgtc gtccagaaat tcgtccacct gctggggagc ccggcccacg tacttggcgg
     3781 ggtcgaggtc gcccgtgcgg gccgacaaat cgagcgcgcg gaacgcggga tcggagtcga
     3841 gtcgttcgag cagatcgttg ggcgtgcccg cctcggccat ggctttcgcg acggtctggc
     3901 tgtgcgaacg aatgacctcg tgcagggtct gccggtcccc ccccgccttc acgcctcgca
     3961 tgaggagcgt ttcggtggcc aggaacggca tctggtcggc cacccgccgt gcgatcaccg
     4021 cctcgcggac ctcgatgccc gccgcgacgt tggtggcgag caccaggatc gcgtccgcgg
     4081 cgaggaacgc ttcgggaatc acgagccgcc ggttggcgct gtcgtccagg gtccgctcca
     4141 gccactgcgt cgcggcggtg tgccaggcgt tgccttctag ctcgatcacg aagcgggcga
     4201 gcgacgtcat ccgctcggcg cgcatcggat tgcgctggta ggccatcgcc gacgagccga
     4261 cctgctcgct ctcgaagggc tcgagcagct cgccctcgtg ctggaggagc cgcaagtcgg
     4321 tggcgaattt cgcggccgat gcggcgatgc cggagagggc ggccaggact tccgcatcca
     4381 gcttgcgcgt gtacgtctgt cccgtgaccg gaaagacctg gctgaagcca agcttctgcg
     4441 cgaccagctg atcgagcttc cggaccttgt cgtggtcacc ggcgaacagc tcgaggaacg
     4501 atgcctgcgt tccggtggtg cccttcacgc cgcgaaaccg gaaggtctcg aggcggtgca
     4561 gcagctcttc cgcgtcgaga atgaaatcct gcatccacag cgtgacgcgc ttgcccaccg
     4621 tcgtcagctg cgcgggctgg aagtgggtgt aggcgaggca ggggatccgg ccggtccggc
     4681 gcgacagctt gcccagcgcg accagcacgg ccgccacccg tccgaggagc aattgaatgc
     4741 cctcgcgcat cagcaggaga tcggcgttgt cggtgacgaa ggcggacgtc gcgcccaggt
     4801 gcaggtaggg acgggccgcg ggggcctggt cgccgagggc gtggatgtgc gccatcacgt
     4861 cgtgccgcaa gcggcgctca tgctcggcgg cgcgcgcgag gtcgacgtcg tcgaggtgtg
     4921 ccctcagctg ttggatcgcc tggtccggga tatcgagacc aagttcccgc tccgactcca
     4981 tcagcgcgag ccagagccgc cgccagagcc cgtaccggcg ctggtcgctc cagagcgact
     5041 gcatcgccgg ggaggcgtag cgcgtgccga gcggggagct gtaggtcaaa cggtcgcctt
     5101 cgcgcggctg gtgagcttgc cccagtcggc caggaactgc tcgaggccgc ggtcggtgag
     5161 cggatgctgg agcagcttct tcaggatgtc gggaggcaac gtcgcgacgt cggcgccgac
     5221 catggcggcc tccacgacgt gccgcgggtg acggatggac gccgcgagaa tctccgtcgc
     5281 gtactggtag ttgtcgaaga tcacgcgggt ctcgcggatc acgtccatcc cttcctggcc
     5341 gacgtcgtcg aggcggccga tgaaggggga gacatagctc gcgcccgcct tggcggcgat
     5401 gaggcactgc acgctggaga agcagagcgt gacgttcgtc ttgatgccct gcttcgagag
     5461 ccgccgcacc gcgaccatgc ccgcctcggt catcggaatt ttgaccacga tgttgtcggc
     5521 gatcttcatg agggactcgc cctcacggac catgccgtcg gcctcgatcg cgatgacttc
     5581 cgccgagacg gggccctcca ccagggagca gatctccttg aggacgtcgc gggggtccac
     5641 gtcgccggcg aacttggcga ggagcgacgg attggtggtg atcccgtcga tcagtcccgc
     5701 ctgggcggcc cacgtgatgt ccttcagatt cgcggtgtcg aggaaaaatt tcatggcgag
     5761 gccccggcgt agaggtcagc gggataccgc acgcacacca ggtacagacc ctggggcggg
     5821 gcgggcggac tggccgcccg gttgtcggtc gcggcgagca aacgagggaa gtcggacgcc
     5881 ggccggcgtc ccagggcgat gtccaccatg gtgcccacga gaaagcgcac catgtgatgc
     5941 aagaaccgat cggcttcgat cgtgaaggtc acccccgcac cgtccgctcg cggcgcccat
     6001 tcggccagcg ccacgcggca tcggtagtga ggtttgacct gtcctgccgc cgcgaatccg
     6061 cggaagtcat gttcgccgat caacgtcctc gcacacgtcg ccagcgcgtc ggcatcgagc
     6121 gcccgatgca gggcccactc gaagcgtctc cggaagggcg actccgccgc cgcgtcggtg
     6181 ccgatcacgt actgataccg ccgggccgtc gcgctcttcc ggggatgaaa gccgggccgc
     6241 atttccacga cccgctccac ccagatgtcc ttcggcaaca gggcgttcag cgcccggtgc
     6301 agaccagtca catcgccggc ccatcgctcg ggggccagga aacccgcgcc ctggcccagc
     6361 gcatgcaccc cggtgtccgt ccgtccagcg ccggtcacca cggtccggcg atcgatgatg
     6421 cgctggagga ccgcctccat ctctccctgc accgtccggc tcgccggctg ccgttgccac
     6481 cccacgaact gccccccgtc atactgcacc accgcgagga aggggcggct cgtcacgcgc
     6541 taaaaataga aaggaggtca gggcctttca aggcacgcaa cccgttgtcc tgtttgacaa
     6601 agcacgattc ttcgggatac ctttggcgac cgtaccgtct cctcatgccc gacacctccg
     6661 cgtcctctcc gctcacgcag gccatcgcca ttctcgcccg gggcgaaccc ctctccgagg
     6721 ccttggccac gcaggtgttc gaagtggtca tgcgcggcga cgcgactgcg gcccagatgg
     6781 gggcgatcct gctggggctt cgcgcaaagg gagagacggc cgccgaggtc gccggtgtgg
     6841 tgcgcgcgct ccgagccacc atggtcaggg tgaccgccga cggcgcccat gtggtcgata
     6901 cctgcgggac cgggggcggg acgatcaaca ccttcaacat ctcgacggtg gcggcgttcg
     6961 tcgccgtggg cgccggcgcg acggtcgcca agcacggcaa tcgctcgtac acgtccctct
     7021 gcggctcggc ggatgtgctc gaggccctcg gcgtccgcat ccctcccggt cctgacgaag
     7081 cggaacgcgc cctggcccgg gcccggatcg cctttctctt cgctccgcac tttcatccgg
     7141 ccatgcgcca cctggttccg gtgcggaagg acttgggagt gacgaccgtg atgaatctgg
     7201 tgggacctct cgcgaatccc gcgggtgtcc gccgccaggt ggtgggcgtc gcggatgcgt
     7261 cccgggcgcc cctcgtggcc gaggcgctcg cgttgctcgg gaccgaacac tccctggtcg
     7321 tgcatgggcg ggccggactg gacgagatat ctcctgatgg cctgaccgac gtctgggaga
     7381 tacgaggggg acggacctcc cactgggtgc tggatccgcg gagcctgccg gttgcgcacg
     7441 tggacctgcg cgacttgcgg gggggacagc cgtcggagaa cgcgcggcgc gccgaacaac
     7501 ttctggacgg cacgggacgg gatgaagcgg ggaagacagc ggtacttttg aatgcggcgg
     7561 cggccgttta cgtcgcgggt ctcgcgcgcg atctggcgga ggggttggaa cgggcacgcg
     7621 ccgccctgga gtcgggagcg gcgcgcgaag ccctgcgaaa gttgcgggat ggggacgagg
     7681 tcagtacttc cgaatgaccc ccaccacgac gccctgaacc atcacgtcgc gctcgttgac
     7741 ccggatgggg gagagctccg cgttggccgg ctgcagcctg atccacccgc ccggctcacg
     7801 atagtacttc ttgacggtgg cgccccccgc gccgccgccc gcgatgccgt tcaagagcgc
     7861 cacgaccgtc tcgccgttat cggcctggtt gcgctcctgg accacgacgt agtcgccgtc
     7921 cttgatgtgc tcgtcgatca tggacgatcc acggacccgg aggacatagc tccgtccccg
     7981 tcgcgggagc agctcgtcgg gcaccgagaa cgtctcgccc tcggtcagcg cctcgatcgg
     8041 ggcgcctgcc gcgacgaggc cgaggagcgg caactcggtg gcgccggtct ggcccttcgg
     8101 gggcacgatc tcgatcgccc ggctttcgtt gtgggtgcgc cggatgtagc ccttgcgctc
     8161 gaggttggtc aggtgctcgt gcaccgtggc cagggattgg aattcgaacc gctccgcgat
     8221 ttcttcgaac gaaggggcgt accccttgtc gtcgatgtgc tcgcggaggt agtccaggat
     8281 ctcgtgctga cgccgggtga ggggcactgc tcactcccga aaagaagccg aatcgaatct
     8341 acccgaatat cgaccgaaag caagcatgcc gaaagccagc attgacgcca ttctcgcgct
     8401 cacacgcacc cgggtgcaag gactccgggg gcgccgggtc gagttggagc ggcgggcggc
     8461 gggggctccc aatcccccgg tcttcgggcc aggcgtcgtc gccagggcgg taggaatcat
     8521 tgccgaagtg aaacgccggt ccccgtcggc aggggacatc cgggcggatt tggacccggt
     8581 gcggcacgcc ctggcttacc aggcgggtgg cgccgtgacc atctcggtgc tgaccgagga
     8641 ggcacatttt gggggctccc tcgacgatct ggagcaggta acgaaggctg tctccattcc
     8701 ggtcctccgg aaagacttta tcgtcgacga actgcagctc ctcgaggcac gcgctgctcg
     8761 agcgtcgctc gtgctcctta ttgtaagggc gctgaccgac gtggagttga ggcgtttggc
     8821 ggccgaggcg accgggctgg ggctcgcgac gctagtggaa gcgcacgatc gggaggagct
     8881 cgatcgggca ttcgccgttt cgccgacagc ggtggggatc aacaatcggg acctgagcac
     8941 cttcgtgacc gatctcagag tcgcggagac gctcgttccg aaggtgccaa aggggattct
     9001 ggcggtggcg gaaagcggaa tagaggaacg tgcagcggtg gagcggctcg cggcggccgg
     9061 agcggacctc gtcctggtcg gcgaagcggt cgcccgcgcg caggatcccg ccctggccgt
     9121 acgaggattg agtggcgtgc cccggagacc gcggtgaccg acgcccaagt agaagggcgc
     9181 ttcgggccgt acggcggacg gtatgtcccc gagacgctca ttgcggcgct cgaggagctc
     9241 gcgcgcactt atgacgatgc gcggcaagac aaggcgtttt gggacgagct gggactgctc
     9301 ctccgggact ttgtcgggcg gccgaccccg atcactgatc tctcgcggat ctcggaagtc
     9361 gtggggaacg gcgtccgcgt ggcctgcaag cgcgaagacc tcaaccacac cggtgcccac
     9421 aagatcaaca acaccctcgg ccaggtcctt ctcgcattgc ggatggggaa gcgccgcatc
     9481 atcgcggaaa ccggggcggg ccagcatggg gtcgcgaccg cgacggtgtg cgcgaagttc
     9541 gggctcgagt gcgtcgtcta catgggggaa gaagacaccc ggcggcagcg cctcaacgtc
     9601 tatcgcatgg gcctcctggg ggcgacggtg gtgcccgtga cgtcaggcac caggacgctc
     9661 aaggacgcga cgaatgaagc cttgcgcgat tgggtcacca acgtggacac gacccactac
     9721 atcatcgggt ccgtggtggg ccccgatccg ttcccccgca tggttcgcga cttccaggcc
     9781 atcatcggac aggaggcgcg ggcccaggtc ctggagcggt acgggcgcct cccgcatacg
     9841 gtggtcgcgt gtgtgggtgg gggttcaaac gcgatgggca tcttcgaggg attccggcga
     9901 gaccaggccg tccggctcgt aggggttgaa gcggccggtg aaggcctttc gggggagcgg
     9961 cacgccgctt cactttccaa aggccgcccc ggcgtcttcc acggcagtct cagctacctg
    10021 ttgcaagact ccgacggtca ggtgcaaccg gcgcattcgg tctccgcggg actcgactat
    10081 cccggcgtcg ggcccgaaca cgcttacctc aaggacacag gccgggtatt gtatcatgcg
    10141 gtgactgacc aagaggctct cgacgccttc aaggatatga gccggctgga gggaatcgtg
    10201 ccggccctgg agagcgctca cgccgtggcc tgggtgctgg ggcaccgcgg ggcgcgggtg
    10261 tgggggccgg acgacgtggt cctgatcaac ctcagcggcc gcggcgacaa ggacgtcgcc
    10321 tgggtggccg aagtgaccgg agcttcgctc tgacgaacgc gcccgtggac gagctgtctc
    10381 tcccgccgtc ccagattacc gagctcttga gtctcatcac caagggcgtt cgggcgaccc
    10441 agctctacct ccccaacaat ccggtctacc agcgggctgt ggacaacgtc cgcggcggct
    10501 tccgtcaggt cttccagtcg gtcgaagaac ttccgctgca gatcaccgag agcgagctca
    10561 agtgggaaga cgaggtcgtc tattccaatg agaacaagag cgagagcttc gcctggctcc
    10621 tgttcaagga cggcctccgc tcactgacgt tcaagcaagg tgtcgaggcg gacgagctgc
    10681 ccaagttcct ccgcatcctg caccaggccc ggaccctgca ggccgacgcc gccgacgaca
    10741 tgctgacgtt gctctgggag caggacttcc agtacatcgc gcacaccttc cgcgagctct
    10801 tgagcgagaa cgcggtaccg atcgaggccg gggaaccgat tcccgaggtg accaagagcg
    10861 acgtcgttca gtacgtgcag gaggaggccc ctccgcggcc caaggggatg gtgtcgatcg
    10921 aggacttcga caccacgctc tacttcctgg acgaaaaaga gatcgcgtac atccactccg
    10981 aagtcgaccg cgagtactcg caggatctcc gcggcaacgt catcgcgatg ctcttcgacc
    11041 tgctcgagct ccagacctat gggacggtcc gcgccgagct gatttcgatt ctggaaaatc
    11101 tgatcccgta tctcctgggc gccggtgact tccggtcagt cgcctatatc ctgcgcgaga
    11161 cgcgggtcat cctgcagcgg gcccgggagc tgatcgccga gcacaagcaa tcgctggagg
    11221 cgttgccgca ccggctgtcg caacccgagg ccctgtccca gctcctccaa tcactcgacg
    11281 aggccacggt ccatcccacc gaggacgagc tctcggggct cttccaggag ctgcggccgg
    11341 aggcactcgt gaccctgatg gggtgggtgc cccggctctc gaacgagcgg gtgaagagtc
    11401 tcgtcgtcgc gtcggcggaa cagctggcgc aggcgaacgc gggggaggtg ctcaaggcgc
    11461 tcgcctccga agacggtgac acgcagctcg agatggtgaa gctggcgggc cggctgaaac
    11521 tggccggtgc gccggacggc atgggacggc tgctcgagat cggcgaacga ccgctgaagc
    11581 tcgcgatcat cgaggcgctc acggccatcg gcagtccgtc ggcgatgcgg gtgatggagc
    11641 gcgccatcga ggatccggat cgcgacgtgc ggatcgccgc cgtgaaattc ctctccgcca
    11701 aggggcaccg caacgccttc aagcagatcg acgccgcggt caacggctcg aagctgaaag
    11761 actcggacct gacggagaag atggccttct tcgaagccta cggagccatg gcgggcgcgg
    11821 cgggtgtcac gccgctctcc cgcatgctgc tctcccggcc caccctgctg gggcgggggg
    11881 aagggcccga gacccgcgcc tgcgcggcga tggccctcgg caagatcagg actcccgaat
    11941 cccggaacgt gctggagcgc gccaaggatg acaaagaagc cctcgtgcgc aacgccgtga
    12001 gccgggccct ccgggagctc ggcgcgtgac ggacccccgg accccttcac cggctccgcg
    12061 ccgcgccacg atttcggcgg agagccaggc ctccgatgcg ttcctccgcc acgcgggccg
    12121 cgagttcctg gtcgtcctct ataccgcgtt ccggtccctg aagctgtatc cgatcgaaaa
    12181 cgcgcaggtg cagaaggcgc tcgacgagct cgcggcgtcc accaaggccc tgctgaacgt
    12241 cgagaacgag ctcgaagtcc gcctgcaggg cgagttcatt tttgtgaact cgacccggct
    12301 gcgcctcgac ctcgacaact acgcctcgtt ctcccacatc ctgggcgtct tccgccagag
    12361 cggcatcggc gccgtccgcg tgctgccggg tgtggaccgc aagcagctgc agatctttgt
    12421 ttccctgctg ctcgcctacg cggcgcgtga cgcggacgcg aacaaggtcc acgagctcag
    12481 ccagaagctc gacgaatcgg gcgtgaacca gatcgacgtc gagccgccgc tggacaccga
    12541 cgacgacgcc gaggatgtcg agaaacagaa agaagcggcg aagcggacgt acgcccggtc
    12601 ggtggccgtg accaaggaag tgatcaacag catccgcatg ggccggaccg ccaacgtgaa
    12661 gaaggtgaaa cgggcggtgc aggccatcgt cgaccaggtg ctgaacaacg aatcgtccct
    12721 gatggggctg accacgctcc gggactatga cgagtacacg ttcactcact cggtgaacgt
    12781 ctgcatcttc tccgtcgcgc tgggccggaa gctcgggctc agcaaggtcc agctctatga
    12841 tgtggggatg tccgcgctct tccacgacgt cgggaagtcg cgggtgcccc tcgaggtgct
    12901 caacaaggag gggggactca ccgacgacga gtggcgtatc atgcaggccc acccgtggct
    12961 cggtgtgctg accctcttcg gactccgtgg ctacggcgaa atcccctacc gcggcatgat
    13021 cgcggcgtac gagcaccaca tgaagaacga cctgaccggc tatccgaagt cgatcaggcc
    13081 ccgcacgctt tccatctatt ccaagatcgt cgccgtggcg gacggcttcg acgcggccac
    13141 cagccgccgc gcgtatcaga ccacgccgat tcagcccgac caggtgctga aggaaatgtg
    13201 ggagaatccg cgccgggggt acgaccccac ggtggtgaag gcgttcatca acctcatcgg
    13261 catctacccg gtggggacct gcgtcattct cgacacgtac gaggtggcgg tcgttcacgc
    13321 cgccaacgcg gacaccgcgt cggtccaccg gcccatcgtc cgcatcgtca cctcccccga
    13381 tggtgggctc ctgcatccgg gattcctcgc ggacctcgcc ctccaggagg acggcgcgta
    13441 cccgcgctcc atcatcaaag tcaccgaccc gctcaagtac ggaatcaagg tccaggacta
    13501 ctttgtctga ttcgcgctcc cggctcgccg agcgctggcg gatggccaag gccgacggtc
    13561 gagccgcgct gatcacgtat ctcacggcgg gctatcccac ccccaaggac ttcgtcgccg
    13621 ggctccgcgc gggcgccgcg gcgggggcgg acatcgtcga agtcggcgtc ccgttttcgg
    13681 acccgctggc cgacggcccc gtcattcagc gcgccacgca cgccgcgttg gggcagggcg
    13741 tcggggtacg ccggacgctc gacctcatcg ccgaggccca gctcgctgtc ccggtcatcg
    13801 tgatgaccta cctgaacccc gtattggcct acggtgtcga ccggttcatc acggatgccg
    13861 cgcaggcggg cgttgccggc gtgctgttga cggacttgcc cgccggcgcc gaccccgacc
    13921 tcgaagcacg cgtcgccgcg gggccgctcg cgttcgtccg tctcatcgct cccaccacgc
    13981 cccagcagcg aatcgagcgc gcggtccagg gggcctccgg cttcctttac ctgatctcgc
    14041 ggctgggcgt gactggagcg cgggaccgca tccccgtgga tctgcccgag tacgtgaagc
    14101 gggtgcgtgc ggccaccgat ctgcccctcg ccgtgggatt cggcatcgga accgcggcgc
    14161 aggcacggga tgccgcgaag ctcgcggacg gcgtggtggt ggggagtgcg ttggtcgacg
    14221 tgctcgggcg ggagggggcc tcgggcgtgg gccggctggt gcgggagttg gcggcgggag
    14281 tgcggggaca ggccgcgtga gcgtcgagac gctggtccgt ctctacgcgc gcggcctcca
    14341 ggtcgccggc ggtctgctcc tgctggcggt cctcgccgcc gaccctcggt ggctgcatca
    14401 ggtcgggacc ctgctggtca tcatcgtcgc gtgcgtcgcg ctgcgcgcgc gccccatcgc
    14461 gctttcgaaa tactcctacc tgacgcagac cgccctcgtg gcgctcgccg gcgcgctgct
    14521 ggtcggcgtg ccggtcacct gtctggggct cgtgctgggg gtcgtgatcg ccgactgggg
    14581 gctccagcgg aaggcgatcc cggtcgcgct ggtgaacgcg ggccgggaag tgatcgccct
    14641 ggtggcgtcg tacggtgtgt acgcggaaat ccggcacctg tcggacaacg tcacgcccgc
    14701 gttctcgacc gcgagcattc cgccgctctt tttcttcgcg ctcgcgtatt tcgcgatcag
    14761 tcgcgtcctc ttctacttca cgctcatcct ccgggccaag ctcgagaggg acgagaagct
    14821 gctgatcgtc cggtacgagc tgatcgggta tggcgcgacg ctcttcgcga cgacgacggt
    14881 ggtgggggtg atgcgggaat ggccgcccgt gtcctggctc ttcgtcgccg ccgtgctggg
    14941 cgccgtaggc ctcctcctca agcgcatgct ggaggaagcg atctccgcgg aggagctcaa
    15001 caagatccac gcgatggaag cggtgatcac gtccaacgtc agcctggagg actccttccg
    15061 gcgcatcgaa ggcctcgcgc accggctggt ggactggggc gacttccgga tcttccgcca
    15121 cgcggacgcg ggcatgcagc tcgcgtaccg gagtgacctg ggccggcccg accggggcgc
    15181 cggggctcct gacggcgggg tcgccgtcct tcggaacgaa gcggtggaaa agggagagcc
    15241 gatcgtcgtc gatgacgtcg gccgggatca gcgcctcgtc aatccgcccg accatgtgcg
    15301 ctccctcgtc atcgtgccgc tcaagttcgg ggaccaggtg atcgggacgc tcgagctcga
    15361 gcaccacaag cggcgtgcct accgggacac cgacgtcgtg acgatctcga ccttcgcggc
    15421 gcagctcgcg acggcgatcc acatcacgga tctgcggcgc ccgctggccg acaccgtgga
    15481 gcgcatgacg agtgagctcg cgaccctggc acggaccgcg gtcgccctcc gcgaggtcgc
    15541 gcgcggcgtg gcgtcggcga cgcatgccat ccggtcgggt gtggtgaacg aagaatcgga
    15601 aatcgcgcgc gggctggaag cgacgaagag cctgtccgag gtgtcggtcc gggtgtcgtc
    15661 cgacggcgcg gcggcggcga agtcgtcgag tgaagcgagt gaaacggcct cccggaaccg
    15721 gcagcgcatc caggacgcca tcgagcggct ggtggcgctg aagacgttcg tcggcgagag
    15781 ctcgggcaag gtgcaggagc tgggagtcgt gagccgccgg atcaccggct tcatcgcgtc
    15841 gattcgcgag ctggccgaca tgacgaacct cctcgccctc aacgccgcca tcgaggcggc
    15901 gcgggcgggg aagcacggca aggggttcgc cgtcgtggcc gaggagatcc ggcacctggc
    15961 cgagcagagc ggggcggcgg cggcggaagc gggcggactg gtggacgacg tgcaccggca
    16021 ggtgggggaa gtggtcgagc agatgcggcg cggacaggtc aacgtgggtg gcgtcgaaga
    16081 gttgtcgtcg ggcgcgctgg acgcgctcga ccggatcgtc gcgaccacgg cggatgcgac
    16141 cgagcatgcg cggcgcatcg cgggcgcggc cgccgagcag aaccaggcct tcgggcgcct
    16201 gaccgagcgg atgaccgcgg tggcggggat cgcctggaag aaccgcgaag aggccgatga
    16261 cgtcgccacc cgcgccacgc aggccgccga cgggctcacc gatctcgagc gcgccaccaa
    16321 agagctcgag ctggtggcga ccaacttgcg tgaattgacg cgcggctttg cgaacctcgc
    16381 ctagggcggt accattctga ccatggcgcg cggggaacgg gtcttcgtgg cgctcggatc
    16441 gaatctggga gaccgcgcgc agtggctcgc ggaggcccgg gaacgcctcg cggcactccc
    16501 cgagacccgc atggtgcgcg cctcaagcgt ggaagaaacg gctcccctgg gggacgtgcc
    16561 gcagggaccg tacctgaatc agatggtgga actcgacacg gggctcgagc cggaggcgct
    16621 gctggacgcg tgcctcggaa tcgaggcggc gctgggacgg gagcggaccg tgcgctgggg
    16681 gccccgccag atcgatctgg acatcgtacg gtacggcgct cgtgccgtgc acacggggac
    16741 tctcacggtc ccccatcccg aattgcccaa ccggcccttc tggcgccgtg agctggccga
    16801 gctgcaggat ctctctgagg attcatgacc gacgcgctga agcaggtcac gacctacgaa
    16861 ctgttgaaga tgaaagagcg gcaggagcgg atcgtgatgc tcacctgcta tgacgcgctc
    16921 ttcgccaggc tcctcgaccc ctccgtcgag atcctgctgg tcggcgactc cgtgaatcag
    16981 gtgctgggcg ggcaggacac cacgctctcc gccacgctcg atcagatgat ctatcacacc
    17041 aagaccgtgc ggcgcgggtc gtcccacgcc ctggtgatct gcgatctgcc gttcctcacc
    17101 tatcaggtga gcccgaccga agcgatgcgg aacgcgggtc gcgtcatgca ggagaccctg
    17161 tgccacggtg tgaagctcga aggcggacgc agcatggcgc ccactgtgaa agccctggtc
    17221 gacatcggca ttccggtgat ggggcatctc gggctcacgc cccagtccgt gaacgccctc
    17281 ggggggtacc gggtgcaggg gcgggacgac gtcaccgcgc agcggctgaa agacgacgcg
    17341 aaggccctcg aggacgcggg cgcgttcgcc gtcgtactcg agctggtgcc gaccccgctc
    17401 tccgcggaga tctcgaagtc actgtccatc ccgaccatcg gcatcggtgc gggtccgtcg
    17461 tgcgacggcc aggtcctcgt cctgcacgac atgttgggct tgaacgaccg cttctcggcc
    17521 aagttcgtga agaaatacgc gacgctggcc gacgatgtcc ggaaggcggc cgaggcgttc
    17581 aaggccgagg tccgttcggg gcgttacccg gacgctgacc actccttcgc gaagtaggat
    17641 cggcgagcgg cccgatgcag gacctcaccg ggctccccga gatgcgcgcc tggacccgcg
    17701 ctcagcgcgg gcgggggaag cgcatcgcgt tcgtgccgac catgggcgcg ttgcatgagg
    17761 gccacctgcg cctgatcgat cgggcgcggg aagttgcgga cctcgtggtg atgtcggtgt
    17821 tcgtgaaccc cctccagttc ggccccacgg aagatttctc ccgctatccg cgcgacctcg
    17881 atcgcgaccg cgtcctcgcg gctgagcggg gcgtcgattg cctgttcgca cccgaccgtg
    17941 cggcgatgta tcccgcggag ccgctcgtcc ggatggatcc ggggccgctg gcgctccgat
    18001 tcgaaggggc ggttcgcccg ggccacttcg cgggggtcct cacggtggtg gcgaagctct
    18061 tccatctcgt cgagcccgat gtcgcggtgt tcggccggaa ggactttcag caggcgatgc
    18121 tcatccgccg gatggcgcgg gacctcgatt ttccgctcga gatcgtgatc gccccgaccg
    18181 ttcgcgagct ggacggactg gcgctctcgt cgcgcaacgc ctacctgaac agtgatcaac
    18241 gccgggcggc cctcggcctc tcgcgaaccc tgcgcacggt cgagcagctg tggcggggcg
    18301 gggacgccga tcccgtgtcc atccggacgc aggggctcct ggtcctgtcg gcggccgggg
    18361 tcgttccgga ctatctggcc ctcgtggatg aagatctcca tgacgtcgat cgcgtcaccg
    18421 ccgcggcgat cgccatcgtg gccggcaagg tcggcaacac ccgactcctc gacaacgtga
    18481 tcctgggcga gggcgtgagc ggagaccctt cgatccgcaa accgtgacgc tgccgggctg
    18541 ggcggtggcc ggtccggaac gacgtgccca cgtggagcgc gtcgcggcgt tgattgatca
    18601 gtgggccgtc gtgatgcgcg tgccggagcg ggaacgaggc cggtgggcca aggcggcctg
    18661 gctccatgac gccctccgtg acgccccggc cccggacgag ctggcccacg gccccgcgag
    18721 tgcggaccgc gcggccaggg agggcgagtc ggaccgcggc gtcctggatg cggtccggta
    18781 tcattcgctc ggcttcgccg gctgggatga cgtcgggaag atgttgtatc tcgcggacta
    18841 tctcgagccg ggccgccggt tcgagcgcga ggcccgggcg gcgctggccg cgcgcgtgcc
    18901 gctggaacgg gacgcggtcc tgctcgaggt cacgcgccgg cgcatcgagt ggaccgtacg
    18961 gtccggctgg cctctctctc cggaaacggt ggaattctgg aactccctcg tcgccccctg
    19021 atcgcgccgc tcgcccgttg gggcgtcgtg accgcattgg ccgtcctggg cggatgccgg
    19081 aacgccagcc cgcggatgtc gggtgacgtc gtccccggcg acggcgccgc cgcgccacgc
    19141 gtgatggtcg ccgtcctgaa tgcgagcggt cgcccgggcc tcgcgcgcct gggaacgcgg
    19201 gtgctgcgcg atgccgggat cgatgtgctg tctttcggaa acagcagtga cccgaagcac
    19261 ccgctcgact cgacgagaat cctggtgcgt cggggtcccg ctgcggttgg ggaacggatc
    19321 cggcgtgcgc tgaaggtggg aaaggtggtg atggaccccg actcgaccct gctactcgac
    19381 gccagcgtgc tcctgggcgc ggacttttcc ccccgtctcc cgctccaccc gtagctggcc
    19441 gcacgcggcg gcgatgtcga gccctcggct cctccgcagg accgcttcaa taccacgttc
    19501 cttcaatcgc cgttcgaacg cgatcatgtg cgtgcgcggg ctgggggtga ggcccggggc
    19561 gccacccggg tgcagcggca ggaggtttac cagggccgcg agcgggctcg ccaccttcgc
    19621 cagtgcctca gcggcttcca gtgaatcgtt cttgcccgcg atcagcacgt actcgatcgt
    19681 gacccgccgc tcgaatccct tcagcgcctc caacagctcg gggagggaat atttcttctc
    19741 caccggcatc agctccttgc gcagggccgg cgtgggcgcg tgcagcgaca cggcgagccg
    19801 gaactgttcg ggacgtttgg cgaagagcgc gagcttgggg agaatgccga cggtcgagag
    19861 cgtgatgtgc cgcgcgccga tgcccaggcc ttccgggtga ttcaggatcg tcagcgcgac
    19921 gtccacgttc tcccaattga gcaagggttc ccccatgccc atgaacacga cgttcgtggg
    19981 ggacagcgcg gggttcttca gcttgatttc ccggacctgg ccggcgatct ccgcgggact
    20041 caggttgcgg ctccacccca tcgtgccggt ggcgcagaac acgcagccga gcgcgcaccc
    20101 aacctgagac gagatgcaca aggtccggcg cttgccctcg gggatcaata ccgattcgat
    20161 cgcctgaccg tccgcgaggg tccagaggaa cttctcggtg ccgtcagacg acacctggtg
    20221 tgcggcgacc gtcaatctgc cgagcgggaa atcctgatcg agcgcctcgc ggagcggagc
    20281 cgggaggtcc gtcgcgtcgg cccatcgcgc gacggggcgc tcccagagtc gagggaggat
    20341 ctgccggagg cggtacgccg gctccccgcg ggtcgccagc cattcgccca gcgcggtgcg
    20401 cgcaccgtcg ggcgtgaggg agagcaagtc ggtcatttga accgggcctc gagtcctacc
    20461 cggtaggacg atcccagccc gctcacgccg gcgtctcgcg cgaatgcaat gccgtagtgc
    20521 ccgatatgga cgcgcagccc cgccacaccg cgccacgcgg tgttcccaaa ggcgctctca
    20581 cggaccagca tgccgtcgat cgcgaacggt gcgccgacgt cgacacccac tccgatggcg
    20641 tgatcggtcc ccacgccgtg cgcgagtgtc gcgccgtacc gaagccgcaa ggcgacctca
    20701 cctgagccgg tccacagcgg gccatgcgcg gcgcgccact cgacgcccgc gtagagatcc
    20761 tgacttgggt cgctggtcgc gaagcggtag aagaagtggg tcgcagccgc gagggtcagg
    20821 acgtcgccga agtgctgggt gacgccgaca tcgccggtcc aatgctgcag gtcggtctgg
    20881 tcgagccgcg agtcgtgcgt gccgagcgtg gcgccgaccg tggtcgagcc ccaggccttc
    20941 gcccaggtga ctcccacggc gcgcgtgtac acctggatcg tgccctgcgg gtcgggggtg
    21001 aacgtggtgt gcgtgatgtc cccgatgtcg acttgtccca ggatcagccc ccaggagccg
    21061 agtctgccgg aaccggtgcg gatcacggcc aggatcccgg ttgcggagat cgcttcggga
    21121 gtctgcacga tgtcgccccc cacactggcg acgctgaccg tttgcgcggg attccaaaag
    21181 gcggccgccg tcccttgggc cagggcgaag gggacagcca cggtcgttcc caccacccgc
    21241 cacaactcgg ccccgacggg gtccccctgc gcctgagtga cccgaggggt ccccagggcc
    21301 aggaccccgg ccagcccgaa tcgccaagtg gcattcacga ctcaacttaa ctagattcaa
    21361 gcgcttacac cacttgagct cacgtgcgcc tatcggaact gttgtccccg agccgcatcc
    21421 gggtccccct caagtcccac gacaaggatg ggatacttgg ggagctcgtg gagctgctcc
    21481 tcccgggtgc gggtggctcc gggggcccag gtgggggtga gcgccaggaa gtcctcgagg
    21541 cgatcctgga gcgggagcgg cagttcccga ccggaatcgg gttcggcgtc gcggtgccac
    21601 acggcaagac gcccgccctt tccagcctcg gcgtggtggc cgggaccgcg acggaaccgg
    21661 tgccatacga aacggtggat ggagaaccgg tccggttgtt cttcctgatg gcgggacccg
    21721 agaccgcggc ggggacccac gtcaaggcga tttcccgcat cgctcgattg gtccgacatg
    21781 aagcggtccg cagccgcctg ctcgcggccc gggacccgga cgagttctat cgcgtgctct
    21841 gcgaagccga agaaggggga ggggcgtgac ggatctggag aagaccgctt ggcggtcgac
    21901 ctactgcggc gcgttgcgcg cggagcatgt gggtcggaaa gtgacgctgg gtggctgggt
    21961 gcatcgccgc cgcgacctgg ggggcatcgt gttcctcgac ctccgcgacc gcgccggcct
    22021 ggtacaggtc gccgtgggac cggcgtgggc cggggccgac gtggtgacgc gggccggcgg
    22081 cttgggcgcc gagacggtcg tggtggtcga gggcgaggtc gtggcacgcc ccgcgaacat
    22141 gaagaatgcg gagctcgcga ccggggatgt cgaggtgcat gcgacgtcgc tccgggtcgt
    22201 gggaccagcc gagacaccgg ccattccggt ggcgcgcggc aagggtgagg agctcgcctc
    22261 cgaggaggcg cggctgacgc accgtcacct cgatctgcgc cgtcccgaga tgcaggcgaa
    22321 cctgattctc cggcaccggt tgctgcagcg cgcgcgccgg accatgtcgg agctcggttt
    22381 cctggagatc gagacgccga ttctcaccaa gcccacgccc gagggcgccc gcgactatct
    22441 ggtgccgtcg cgggttcacc cgggcgagtt ctacgcactg ccgcagtcgc cccagatcta
    22501 caaacagctc ctgatggtgt cggggttcga tcgctacttc cagatcgcgc gctgcttccg
    22561 ggacgaggac ctgcgcttcg accggcagcc ggagttcacg cagatcgaca tcgaggcgtc
    22621 attcgtcggc cccgaagacg ttcagcggtt cgtcgaagcg gtgctcgtgg cctgctgggc
    22681 cgaagcgggg cagtccgtgc cgtcaccctt tccgacgctg acgtggaagg acgcgatggc
    22741 ccggtacggg accgacaagc ccgaccttcg ctacgacatc cggttgggag actggacccg
    22801 ggagttcgcg agctggaccg acctgccgct cgcgggggcg ctggcgtcgg acccgaagaa
    22861 ccggctcgag ggcttcgtgg tgccgggtgg tgccgcgctg tcgcggaagg aaatcgatgc
    22921 gctctccgaa atcgcgaaga aggcgatcgg gaagacggtg gtctgggtca agcgtcaggg
    22981 ggaccagatc tcgggatcgg tgaagctcac ggcggagcgc gccgccgcgc tcggcgtggc
    23041 ggacggcgcg atggcgatca tgctcttcgg cgcgccctcg gagatccgtc cggcgctcgg
    23101 gaccttgcgc gccgaggcga tcaagcggct gaagctcgcg cccagcgcga cgcacgcgtt
    23161 ctgctgggtg gtggatttcc cgctcttcga acccgacccc gaaacgggga agccggtgtt
    23221 cgcgcatcac cccttcacgt cgccgcatcc ggacgacgtc gcgaagctcg acgccgagcc
    23281 cttcgcgtgc cgggctctgc actacgacgc cgtctacaat ggcaacgagc tcggctccgg
    23341 ctcgatccgg atcacggatc ccgcggtcca gcgaacggtg ttccgtcacc tggggctcac
    23401 gcccgcggag atcgacgccc ggttcggatt tctgctcaag gcgctcgcgg cgggcgcgcc
    23461 gccgcacggc ggattcgcgg tcgggttcga ccgcgtgacg atgctgctcg cgcaggccac
    23521 ttcgctgcgt gatgtgatcg cctttccgaa gacgacggcg gcccgtgcgc tgttcgaggg
    23581 ggcgccgtcg ccggcgaacc ccgcggacat cgcggcgctg catctggagg tgactcgatg
    23641 agtgacgacg tcattcgccg gttgtcggtc gagggagtgg atcccctgac gctggccggc
    23701 gtgaacgatg cgaatctgat cgagctgtcg aagcggctgg gcgtccgcgt ttccctccgc
    23761 ggggacacgc tcacgctgca gggcaagccc gaagccgtcg accacgccgt tcccatcacc
    23821 caggcgctgg tcgatctggc gcgggtcggg gacccggtgg acgtgcacga cgtcgagcgg
    23881 atcgtgctcg aggacggcgc gccggctgcg gcgggagaag gcggagaata caagatcatt
    23941 ctgccgggac tgcgccgggt catccaggcg aagacggcgg gccagcggga atacctgcag
    24001 gccattcagc agaaggaaat cgtcgtcggc atcggaccgg cggggaccgg gaagacctac
    24061 ctcgcggtcg ccgcggcggt ggatgcgctg gcccggcacc gggtacgccg gatcattctc
    24121 gcgcgcccgg cggtggaagc gggagagaac ctggggttcc tgcccggcga tctgcagcag
    24181 aaggtcgatc cctacctgcg tccgctctac gacgcgctcg aggacatgat gcctcgggac
    24241 cgcgtcgaga aggccctcga aacgcgggtg atcgagatcg ccccgctcgc gtacatgcgc
    24301 gggcgcacgc tctcggacgc gttcgtcatc ctggacgagg cccagaacgc cacgggcatg
    24361 cagatgaaga tgtttctcac ccggctcggc gtgaattcac gcgccgtgat cacgggtgac
    24421 aagacccaga tcgaccttgc caaccgcgag gactcggggc tcctgcaggt ggagcggatt
    24481 ctgccgggca ttgacgggat cgcgttctgc tacctgaccg acaccgacgt ggtgcggcac
    24541 cggctggtgc gggagatcat caaggcctat gcggaagaca gtcaaggctg acccggtccc
    24601 ccgtgaagag cgcatccgct accacctgat ccggtggtgg tgggtgatcg ggctcgcggt
    24661 gctggccgag acggcgtttt cgttcggcgc ggggggcgcg gtcgacgtcg agaccatgct
    24721 cggcgtcatg ctcgagaacg cgctcatcct ctgcgtgttc tgggtgctgc tcttctacta
    24781 ccgccgggag acgtaccggg acaaccgtca ggtcgccatg atcggcggcc tgttcgcgtt
    24841 catgatcatc atcgccgcca tcatcggagc cctggccccc cagcaccccg agctggttcc
    24901 gctgcccctc ttcgcgatga ttctcacggc gcagttcaac ggccgggtct cgatgatcgg
    24961 cgccatgatc ctcgcggtgc tggtggacat tcagccggtc ttccacaatg gacccgcgtt
    25021 gttcgtctgt ctcgccggcg ggatcaccgc ggcgctgtcc gtgcgcaccc tcaggcgccg
    25081 cagccatctg tacctcgcgg tcctcatcac ggcgatcggc tatctggacg ccgcgatcgc
    25141 gctcggcctg gtgggacact ggccgatcgc gcagatcggg tggatgacgc tcctcggcgc
    25201 cgcgaacgcc ctcgtctcgg cgtcgctcac gatcctcctc ctgccgctca tcgaaagcgt
    25261 cacgaccgtc accaccgacc tcacgctcct cgagctctcg gacccgggcc ggccgctgct
    25321 ccggcggctc tcactcgagg cgcccggcac ctacgcgcac agcgtggcga tggccaacct
    25381 cgtcgaagcg gcgtgcgacg cgatcggcgc gaatggattg ctgggacgcg tcggctgcta
    25441 ctaccacgac gtgggcaagc tcgggaaccc gggattcttc gtcgaaaacc agaatcgcgg
    25501 gaccagcaat ccccacgacc ggatcacggc gcagcagtcc gcgaagatca tccggcaaca
    25561 cgtggtggat ggcgtgcggc tggcccagga ggcgcgcctg ccggaaatca tcaccgcgtt
    25621 cattccggag catcacggca cgtccgagat caactacttc ctcgacaagg cgaagaagtc
    25681 ccgggacggg gtagtgaatc cggcggacta ccgctatccc ggcccccggc cgcgctcgat
    25741 cgagacggcg atcgccatgc tggccgattc ggtggaggcg tccttgcgcg tgctcgacga
    25801 tctcacgccc ggccgcatcg agggcgtgat cgaccacctc gtgaagaaca agctcgcggc
    25861 gggccagctc gacgaggccc ccattacgct tcagcagatc gacgacgtga aagcggagtt
    25921 cgttcgcgtc ctgtcgggga tgtaccacaa ccgcattgac tacccggaag atcgtggcgg
    25981 catctcggcg ggctggcaga gctcgcagcc gtcggcgccc gccgccggcg cctgagcgcc
    26041 tcctcttgac cgcggtgcgt cgggtgctgg cctgggaagg cgcgcccggc gcgcgcgttt
    26101 ccgtgaccct gcttcccccg gcccggatga aagcgctcaa ccggcgcacc ttcggacacc
    26161 ggggcctcac ggacgtgatt tccttccccc tgccacagcc tgacggcacg ctccacggcg
    26221 acgtgtacgt gtgtccagcg gcggcgcgag cctgggtcaa ggacgcgggg cccggcgcgc
    26281 tgcgggagga gatggtccgg ctggtggtgc acgggaccct gcatgtgctg ggctacgacc
    26341 atccgtcggg gccggggcgg acgcgctccc ggatgtggcg gacgcaggag cggtacgtgc
    26401 gtcggctggt gggggcgggg aagtgaccgg gggcgggggc gcgctgctgc tcgtcgaggc
    26461 gctcatcgcg ctcggcgccg cgctctgggc cggactcctg tccctcggcg aggaggccgc
    26521 ggtcggcgag acggcgcata ccatggcgga cgcgcccccc gtcgcgcggg ttccgctgca
    26581 ccgggggctc cacgtcgcgc ggctcgcgct gctggtgctg gcggcggtcg cgggggccag
    26641 cgccctcagc tggtggaccc ggggaccgat gggcgggctg gtctccgtcg ccgtcgcaac
    26701 ggggttcctg ttcgtggtcg gcgatgcgct gccgcggagc ctggcgagtc tcgcgcccga
    26761 gctcgcggcc gccatcctgc ccctcgcgca ccgctcgctc gcggtcttcg acttcctgct
    26821 ggcctttctc gggtgggccg accgcggcct gcacggcctc gtgcacttgc cgcggcccgc
    26881 gtctcccgtg ctcgggggcg cccagcgcga catgctgctc ggcgtgttca tgatggcgga
    26941 cacgaccgtg gacgaagtga tgacgccgcg actcgacctg gtggggatcg atgtgtcggc
    27001 cccctacgac gaactgctgc gcgtcttcaa ggagagcgga cacacccgcc tgccggtgtt
    27061 cgacgggagc cccgacgccg tccttggcgt cgtgttcgcc aaggatctgg tgccgatcgc
    27121 gctcggcttg aagccgcgcc ccgatgaatg gcgggagctg gtgcgctcgg tggcgtacgt
    27181 gccggaggcg aaagtgctcg acgcgcagct gcacgatttc cagcgcggcc gcgcgcacat
    27241 cgccatcgtc gtggacgagt tcggcggcac gtccggcatc gtcacgctcg aagacattct
    27301 cgaagagatc gtcggggaga tccgcgacga ccccgcgacg gagccaccgt ccatcgagca
    27361 ggatggcgac cgctactggg tgcatggggg cgtcgggctc gaccagctct cgcagaccct
    27421 gggcgcggat ttccacaatg ccgaggtctc gaccgtcggc ggcctcatct actccgtgct
    27481 gggccgcgta ccgcgctcgg gggaggaact gcacctcgac ggatttcggg tcgtcgtcga
    27541 gcgcgtggat cgccgccggg tcacccgcgt ctacttcgag cgcgaatgac cgccggcctg
    27601 ttgctcctca ctctggcgct gctcgtcgcg gcgacgtccg ccaccatcgg cgtcgccgcc
    27661 gcgtccgtga gtcaggcgga gttgggacgc tgggtgagct tgcggctccg gggctcgggg
    27721 tcgacgtccg cgctggtgga gaacccgggg cgggttctgg cgaccgcgaa cgcgctcacg
    27781 actctgggcg tgctggcggc gggaacggcg gtgcccgcgc tcttcctgga actggcgccg
    27841 acgtttctgg gcatcgtcac gttcgccgtg gtcgtccccg tgttcgtggt ggcgtcgtac
    27901 gtcgtcccgc gtgtgttggg ccggcggtgg gccgagcgga tcgtctcgcg gaccctcccc
    27961 gcgatcaact ggttgagcag tgcactcggt cccttcctgc cgcgccggga cccgtcgacg
    28021 cgtcacgcgc tcgcggccat gctcaccggg gcggggtccg acgccctcgc gggtgtggac
    28081 gagctcgaag tggtctccgg ggttctggcg ttcgccgacc gcccggtgcg ggacgtgatg
    28141 acgccccgca ccgccatcgt cgcgcttccg gggggcacgg ccgcgggtga ggcggctcac
    28201 gtctttctgc agtcgggcta ttcgcggtat ccggtcctgg gcaagtcact cgacgacatc
    28261 gtcggggtca cgtactcgaa agatctgctc tggctcgatc cgaacgcacc catcccggtc
    28321 cggccgatcc tcaccgtgct ctccggacgt cccgccgccg atctcctgct cgagatgcaa
    28381 cgcgggggcg cccgtctggc cgtcgtcgtc gacgagttcg gggggacggc cggcgtcgtg
    28441 accttcgagg atctgttgac caccctcatg cgcgacctgt tcccgctgga gccgcaggcc
    28501 ccggaaaagg agactcccat cctcgaaatc gagggcgggg cgccggtgga gcggttgctg
    28561 gagcgcttcg gccatccgcg cgaggcccgc ggggcgcaga cggtcggcgg gtacctcacg
    28621 cagctcctgg gccgcatccc caaggccggt gagcggattg ccgtcgaagg cctcgagttc
    28681 gacgtcgtgg cggcctccac ggtccgaatt gagcgagtgc tcgtgcgttc cggccccgtg
    28741 cgcgtcaccg cgctcgattc cagggctgac gcgacccccg gaagcgttta gcttccatgg
    28801 agatggggac cgagagcttg ctcgaggggc tggagcggcg ggatcctgcc gccttggcgc
    28861 gcgccatcag cctggtggag agccggcgcc ccggctacga aggggtgctc tcgcacgtcc
    28921 atggccgggt gggcgccaag ggcgccaggc gcatcgggat aaccggaccc ccgggggcgg
    28981 gcaagagcac gctcaccgag cggctggtgc agcacttccg gggcgccgga ctcaaggtcg
    29041 ccgtggtggc ggtggatccg accagtccgt tcacgggcgg cgcgctgctc ggggaccgca
    29101 ttcggatgga atcggtgagc ctggatccgg atgtcttcat ccgttccatg gcgacccggg
    29161 gctccctcgg cgggctcgcg atggcgaccg aggaggttgc cgacgttctc gacgccttcg
    29221 ggttcgagcg gattctcatc gagacggtgg gggtggggca gacggaattg gacatagcgc
    29281 ggacggcgga gagcaccgtg ctggtgctcg tcccggagtc cggcgacgcc atccagacct
    29341 tgaaggccgg cgtgatggag atcgcggata tctacgcggt caacaagagt gaccggccgg
    29401 gtgccgaccg gctgcggcag gaaatcgaag tgatgctcgg gatcaggcgg ggcaatgcct
    29461 ttcgccacgt ctccgcgcac cacaagcccg accctaaagg accggacgcc cgggcacgca
    29521 agggcgtgca gcaatcggct gccgccgccg agtgggagcc accggtgctc gcgaccgtcg
    29581 catcgcaggg agaaggcatc ggcgacgtgg tggcagcgct cgaccggcac cggacgtacc
    29641 tggaatcgag cggcaagctc ctggagcggc gccgccgtcg cctgacggaa cggacgcgct
    29701 cactggtgga gcggcatctt gcggaatggc tgtgggacga gtcggcggcg ggagaggtac
    29761 tcgaacgccg gctggacgcg atggtggagg gagaccgcaa cccgtacgag gtggcggcgg
    29821 agatcctgga ccaggtgaag ggcggagtga ccccatgagc atcgaacgca agccggtcta
    29881 cacccccgat gacctcgcgg agttcgaccc cgcgaaacag ctcggcacgg cggggagctt
    29941 ccccttcacc cgcggcatcc acgacacgat gtaccggggc cggctctgga ccatgcggca
    30001 gttcgccggg ttcggaaccg ccgaggatac caatcagcgg tacaagttcc tcctcgagca
    30061 cggacagacg gggctgtcgg tggcgttcga tttcccgacg ctgatgggct acgactccga
    30121 tcacccccgg tcggagggcg aagtcgggaa gtgcggcgtc gcgatctcgt cgctcgcgga
    30181 catggagacg ctgttcgacg gcattccgct cgatcaggtc tcgacctcga tgaccatcaa
    30241 cggtccggcg gccatgctct tctgcttcta cgtcgcggcg gcggagcggc agggcgtgtc
    30301 catcgagaag ctccgtggca ccgtgcagaa cgacatcctg aaggagtaca tggcgcagca
    30361 tgcgtgggtg tatccggccg agcccgcgtt gaagatcatc gtcgacatgt tcgagtggtg
    30421 cgccgagcac acgccccagt ggaatacgat ctccatttcc ggttatcaca tccgcgaagc
    30481 gggtgcgacg gcggcgcagg agctcgcgtt cacgctcgcg aacggattca cctatgtgga
    30541 gcgggggatc gcacggggcc tcgacgtcga caccttcgca tcgcgtcttt ccttcttctg
    30601 ggacatccac aacgactttt tcgaggagat cgcgaaactg cgggccgccc gccggatctg
    30661 ggcccgtcac atgaaggacc ggtaccgtgc caagaacccg cgctcgtgga tgatgcggtt
    30721 ccattcgcag accgccggtg tgacactcac ggcgcagcag ccgctcaaca acatcgttcg
    30781 ggtcgcgtat caggctctcg ccgcggtcct gggcggcacc cagtccctcc ataccaattc
    30841 actcgacgaa acgctggccc tccccaccga acaggcggtg caggtggccc tgcggaccca
    30901 gcagattctg gcccacgaga ccggcgtgcc ggaggtgatc gatccgctgg gcggctccta
    30961 ctacatcgag tcgctcacgg atcagctcga gcgtgaagcg gaggccctgt tcgccgagat
    31021 ccagtcgctg gggggcgtgg tgccgggcat cgaaaccggc tggttccagc ggcagatcgc
    31081 ccggtccgcg gccgagcagc agcggaacgt cgaggacggg aaatcggtga tcgtcggggt
    31141 gaacgagttc gtcgcctccg aagatcatcc cgtggagatc ctcaaggtct cgggcgaagc
    31201 ggaagactcg cagaaggcga agatggccgc gctccgggcc cgccgggacg acgcgctggt
    31261 caagtcacgc ctcgaagccc tgcgggtggc ggcgcagacc gatcagaacg tgatcgggcc
    31321 catgctcgac tgcgcccgcg cctattgcac gctcttcgaa atccggcatg cgctcgaaac
    31381 cgtgtacggg gcctaccgcg agccggtgtt cttctagaaa tggcgaacag caagaagcgc
    31441 ccgatccgcg tcctcgtcgc caagcccggc ctcgatggac atgaccgcgg ggccaaggtc
    31501 gtcgccgccg cgctgcggga cgccgggatg gaagtgatct acacgggtct ccaccagaca
    31561 ccggagatga tcgcccgcgc cgcggtccag gaggacgtgg atgtggtcgg gttgtcgatc
    31621 ctctcgggcg cgcacctcac gcttttcccg cgggtgaagc agctcctcaa ggacgcgggc
    31681 cgccacgatg tgctcctgac cggcgggggc atcattccgc cggaggacgt ctcccgcctc
    31741 gaggccgaag gcgtcggcaa actcttcggc cccggcactc cgacctccga gctcatcaag
    31801 tacatcgagg agtgggccgc tacgcatgtc gccgcgtaga tgagtcgcct ccaagcgctg
    31861 accgccgaat accgcgagct caagtcccgg atcgaagcgg gcggcggccc ggacaaggtc
    31921 gccaagcagc acgaccaggg gaaattgacg gctcgcgagc gagtctcggc cttgctcgac
    31981 caaggcggcc cctggctcga gatcggcctc ctcatggcgt tcgatcagta tgacgggcag
    32041 gcgccggcgg ccggggtggt caccggactg ggccgggtgg gcggccgtcc ggtcgtcgtg
    32101 gtcgccaatg acgcgaccgt gaaggccggc tcctggtggc ccgaaaccat caagaagatt
    32161 ctgcgcgcgc aggaaatcgc catgcggcag cgcatcccca tcatctatct ggtggactcg
    32221 gcgggcgtga acctcccgta tcaggggggc gtctttccgg ggcagtacgg cgccgcgcgg
    32281 atcttctatt acaactccat catgcggcac tatctccggg tgccccagat ctccgccgtc
    32341 atgggcccgt gcatcgccgg tggcgcgtat ctgccggcgc tctccgacgt catcttcatg
    32401 gtcgagggca cgtcgttcat gggcctgggg ggacccaatc tcgtgaaggg cgcgaccggg
    32461 cagacggtgg atgccgaaac gctcggtggc gcgagtacgc acacgagcgt atccgcggtg
    32521 gcgcactaca aggccaagaa cgatcaggag tgcctcaccc gcatccgcga atacgtgggc
    32581 aggctgccgc gggcgacccc ggcggtattg aacggctcgg cccccaagaa gaacggcaaa
    32641 gccctgtacg acgcgctgcc cgccgaccac cggatgagct acgacacgca tctggtgctc
    32701 gagggcctgc tcgacggcgg tgccttcgac ccctttcagc ctgacgtggc accggagatc
    32761 ctctgcgggc atggccggat cgccggccgt ccggtggcgg tcatcgcgaa cgcgcgccag
    32821 ctgatcaagg ggaagaaggg agaacccccc aaaattggcg gcatcattta cacgcagtcc
    32881 gccgagaaga ccgcctattt catcgagaac gcggaccgcg aacgcatccc gctcctgttc
    32941 gtgcaggacg tttccggctt catggtcggg actgacgcgg agcattcggg catcatccgc
    33001 gccggcgcgc atttcgtcga ggccatggcg acggccaccg tgccgaagat cgtgctgacg
    33061 ctgaaccacg cgtcaggggc cggttactac gcgatggcgg ggcagggctt cgacccggac
    33121 ttcatcctct cctggcccac gggccgcatg ggagtgatgg aaggagagtc cgcggtccag
    33181 gcggtgttcg gcgcgggggc ggcgaagccc gagaacgccg gtgccgtcga agccatgcgg
    33241 gccgattacg agcatcagct cgacgccaag tatgccgcgg cgcgcgggta tgtcgacggg
    33301 atcatcacac ccgaggagac ccgtgacatg ctcgccttcc tcttcgagac cgcggccgag
    33361 tacgcgggcc cccatcttgg gacattctcc ctgccggagt ctcgttgatg gtgcggcgcc
    33421 ccgtcgggtg ggccgccgcg gggctgacgt tgtctctggc ctgcgccggc gccaaaccca
    33481 cgtcagacgt cacgccgcgc agcgccccgg cgccatccac caccgcccct gtggaaagtc
    33541 cgtccccacc cgttcccgcg ccggtggacg tcatgcccgg cttgcggccg gtcatgtctc
    33601 ccgcggctgc gtacctcgcc gggatgctgt cgctccggag tgccggcgtg gatcgctttg
    33661 ccgccgcgca tcccacgtat gatggacgcg gggtcatcat cggcattctg gacactggtg
    33721 tagatcccgg cgttcccgga ctgatcacga cgagcaccgg tgcgccgaag gtcatcgagg
    33781 agcgggactt ctccggagag gggcaggttg cgctcacccc cttcacgccg gagggcgacc
    33841 acgtctccat cgggggccat acgttcggtg gagcgggccg gatcggccgg atggccgtgc
    33901 tgagcacctg gtacgggggc ttccttcgcg agatcaacct cggtcccgct ccgggcgggg
    33961 atttgaacgg cgatggggac aacaccgacg tctttcccgt cgtcgtggtc aaggcgaccg
    34021 acggatgggt ggcgttcctg gactccaatc tgaacggatc gttcgaggac gagatgcccc
    34081 tccacgatta ccgtgagggc cgtcagacca tctcgctggg cagcaagccg gtgacgctgg
    34141 ccgcgaattt cgcggaggtg aacgggacgc cggtggtcga catcgtcggg gacaacctgg
    34201 cgcacggcac gcacgtcgcc gggatcgccg cggggcacaa catctacaac gtcgcgggct
    34261 tcgacggcgt cgcgcccggg gcccagatcc tggcgttgaa gatcgcgaac aacgcgcgtg
    34321 gcgggatctc cgtgaacggc tccatgatcc gcgccatgcg gtacgcggcg gcctacgccg
    34381 ccgcccgggg cctgcccctc gtcctgtccc tgagtttcgg cgtgggggac gagcatgacg
    34441 gcccggtggt catcgactcg ctggtgaacg aattcctgaa gacccacccg gacgtcgtcc
    34501 tggccgtcag cgcgggcaat gacgggccgg ggctctccac gctgagctac ccgggctccg
    34561 ccgatctggc gctggccgtc gggtccctcc tgccggggtc gttcgcgaag ccgacggtgc
    34621 ccggggacgt ccccactccc gacgtgatgg gggactggag ctcacgggga ggcaacgcgg
    34681 ggaagcccga catcgtgacg cccggtgtgg cctattccac ggtgcctcgc tgggacaccg
    34741 ggcacgagat caaggtcggc acgagcatgt cggcaccgta cgccgccggc ctcgtcgccc
    34801 gcctgctctc cgccatgagc caggagcaac ggcacgtcac cgcggccgac ctcatcgcat
    34861 ccctccgggc caccgccgct cccctggcgg gatggacgtc gctcgacgac ggcgccggaa
    34921 caccccggct cgaggccgcg tatcgctggc tcgtcgccgg ccatcagggc gcgcgatacc
    34981 aagtgttcgc ggatcagggg agctccggcg gctttcgtcg cgacggactc gccgcgggtg
    35041 acaccgttgc ccacttccga atcgtgcatg tcgacggctt tcgcccggcc cacgtcgacc
    35101 tcaaggacga tgcgtcctgg atgtccgtgc cgtcagtggt cacgtcggat ccactgggaa
    35161 tcaatctctc ggtaccgatg gatccgcggc ggctgacggc acctggtctc tatgtcggga
    35221 cggtcacggc gtcggttccg gatgacacgc tggcgggagc caccttcacg gtgaagaaca
    35281 cggttgtggt gccctacgat ctcacgcggg ggccgctgtc ggatttgcat cgtcgcatcg
    35341 taccctcgca ggtgcgtcgc tacttcctgt cggtgcccac gcccggcgcg acgctgtcga
    35401 tcgcggtacg gggctcaggc ccgtcggacg ccgcgaccgt tcatctttat gagcctccgg
    35461 gccgcccgtt tcgggacggc gacaaggcgg aaacgggacc cgacgcggcg ccctgggcgg
    35521 cgttccgcgt gcggggtgag gatctggtgc ctggcgtcta tgaactggac gtgacggctc
    35581 cgccgctcac gacgacgaat gtcgacgtcc acgccgagct cagctccgcg tcgctcgagg
    35641 tcaccgggag cggaatcgag atctccaacg cgacctcgtc gagcatcagc ggaaccctct
    35701 ccgtgcgtgt gatcggtgcg gagcgcgaca tccaggtggc ggggcggggt gccccgccgg
    35761 aatcgatcgc cgtgcaggtg cctcgctggg ccgcgcgcgc cgaggtggac gtggagatgc
    35821 cgcgggcgca gtggggacgc tttacggact tcggagtcac cgtgtacgac accgctggac
    35881 agcatctgaa cgacgccccc ctcaactacg cgatcgggcg gcaatccttc gaagtgccgg
    35941 ccggaatcgc cggtgccccg gcgctcatcg agctatttcc cgcgctggcg ctcgtcgacc
    36001 gggccgggcc ctggcaggcg acggtgcgcg tccggttcct gatggacagc gcaagtgcca
    36061 ccggtggcgc gcgccggccg atcgccgtgg tggccggggg ccgctcggcc gtcgaaacga
    36121 acgatctcag cccgccggca ctccccgagg gattccatcc cctgctggat gcccgggtga
    36181 cggtaccagg atcatcggac gcggaacggc gggtggtgat gggagcgacc ggcggggtgg
    36241 cccgatgacc aagtccgtgg tgcgcgtggc cggggcgcag ggcttctggg gtgattggct
    36301 ggaggccccc tatcgccagc tggtcggcgg gccggtcgac tacctcatgc tcgactacct
    36361 cgccgaagtg acgatgtcca tcatgcagaa acagaagtct cgcgatccct ccttgggata
    36421 cgcgaaggac ttcatcccgc tgatggagcg gattctgcct gagttggtga agcggaaggt
    36481 gaaagtcacg agcaacgcgg gtggcgtgaa tccccgcgcg tgcgccgagg cggtcgcgca
    36541 ggcggctcgg aagctgggcc tggcgggcaa gctgacgatc ggcgtggtga cgggcgacga
    36601 cctcctgccg cgtctcgatc agctgctcgc ggccggccat ccgctcgcca atctggatac
    36661 cggccgcccc ctcacggacg tccgggccaa ggtgcagtcg gcgaacgcgt acctgggggc
    36721 agcgcccgtg gttgaagccc tccgtcaggg cgccgacatc gtgatcacgg gccgtgtcac
    36781 cgatacgggt ctcacgctgg gtcccctgat gtacgagttc ggctggcgcg ccgacgactg
    36841 ggacaggctg gcggccggca cggtcgccgg gcataccatc gaatgcggtg cccagtccag
    36901 tggcggcaac gacctcgccg actggggccg ggtcccggac ctggcgaatg tggggtaccc
    36961 gatcgtcgag gctcgcccgg acgggacgtt cgcgatcatc aagcatcccc ggacgggcgg
    37021 tgcgattacc gtggcgggtg tcacggagca gctggtgtac gaaatgggtg acccgcactc
    37081 gtacatcact cccgactgtg tggccgactt caccaccatt cagctgaaac agaaggggaa
    37141 ggaccgggtc gagttctcgg gaatccgggg caagccggcg accgacaagt tcaaggtctc
    37201 ctgcgcgtac ttctacgggt acaaggccgt agggacgctc gtgtacgcct ggcccgacgc
    37261 ctatcggaag gcacaggcgg ccgaccgcgt gctccgccag cgcctgaagg acctgggtct
    37321 gtcgtacgag accgtgctga cggaattcgt cggggtcgac gcgacgcatg gaccgctcgc
    37381 gggcgcgccg agccccgatc tggcggaggt gcagctgcgg gtgggggtgc gggcgtccga
    37441 caaggcgccg gtcgagcggt tcactcgtga gatcgcaccg ctgattctga acgggccacc
    37501 gtccgtcacc ggttttgccg gcggacgccc caagccggag gagatcgtcg cctactggcc
    37561 ggccctcctc gacaagcgtg aggtgaccgc atgcgtcgat ctggtgcgcg tatgaagaag
    37621 aaatcgaaac cgaagcttac gcctttggtg gccattgccc acgcccgctc cggggacaag
    37681 ggtgacacgg cgaacgtcgg gctgatcgcc aggcaggcga agcactatcc gctgctggtg
    37741 aaggaagtga ccgcggcccg ggtgcggcgc catttcaagg gcgtgatcac cgggcccgtc
    37801 gagcgctacg agctgccgaa cctgaaggcg ctcaatttcc ttctgcacgg cgcgctcgac
    37861 ggcggtggca ccatttcgct caagaccgac gcgcaaggga aggtgttctc cacggccctg
    37921 ctgcgcatgc ggatcaaggt gccccgatga gtgatcgtgt actcgtcaac ctgaccgaag
    37981 gcattctcac cgggacgttg aatcgccccg acaagaagaa cgccatcgac acggccatga
    38041 tggaggcgct ggcagagctc ctcgaccagg ctgacctgga cgctggggtc cggctgctgg
    38101 tgatccgcgg cgcggggaag gatttctgcg cggggatgga cctgaacgag cttctggcgt
    38161 ccgccgatca aacggtcgag cagaaccgcg ccgccgcgct ccggttcggg aatctgttca
    38221 tcaagatgcg cgccctgccc aagccggtcg tggcggtggt gcagggacgg gccttggcgg
    38281 gcggctgcgg gctggccacc gcctgtgatc tggtgctggc ttcggaaacg gcgcagtttg
    38341 gctacccgga agtgcagcga ggatttgtgc cggcgctcgt catgaacatg ctgaagcggg
    38401 ccgtggggga aaaagtcgcg ttcgacctgg ccgcgacggg gcatctcctc aacgccgcgg
    38461 aggcacacgc ggtgggactc gtgtcccggg tgtacgacga ttccgacttc gaggagcagg
    38521 tgggcgacct cctgcgcacc atcgcggagt cgagtggcac cgccctcgcg ttcaccaagc
    38581 ggcagttcta ccagctggac ggcgtgtcgt tcgacgccgg catcaggctg tccgcggacg
    38641 tcaacgcggc gagccgggcc accgccgact tccgcgcatc gatcgccagg tttctcaaga
    38701 aatgaccgaa cgcccccgcg ggttcacaca gcccaaggcc gccgccgacc tggtcaagag
    38761 catcgttcag cagaaggtcg aggacgcgaa acaggtcaag gccgaggccg agaaggcccg
    38821 caagaagaac aagagcaagg tgccgctcct gctgggcctg acgccggtct tgttggggct
    38881 caccgcgtgg aacgtcctca gctccggccc gccggagtcg aagctcaccg ccgccgatca
    38941 gctcgcctcc accaagttcc ggatttacat cgcggcagag gcgatcgagg ggtaccgggc
    39001 cgcgcacggg gtgtacccag cgaacctgac gcaagtcggc gtcgactggc aggggctcca
    39061 ttacgcgaca tccgacacga cctggagtat cgtcgcgcaa gcggataccg tgaccgtgac
    39121 ctaccggcac ggagacccga ttaccccgtt cgctaccgcg tatcagtcat tgcagcggag
    39181 gaagcggtga agggaggcga ccgtcgcggg ttcaccctca tcgagctcct ggtcaccctc
    39241 atcatcgtcg ggatcctcgc gaatatcgcg atcccgctga tgcgggtggt gaaggtccgg
    39301 gccaatgcgg ccaaggtgct gtcggacttc gacgtgatcc gcggggccgc gtacgacgtg
    39361 tacgcctcac atgacagctt tccaccgagc ggcaccttcg gccaggttcc gccctggttc
    39421 gtgcagtccc tgcccaaagg gttccagttc cagtatcagg acgttcagta ccgctggtcc
    39481 ctgctcggaa ccccccaggg gaacccgctc tcaccggggc agcccctcct cggactcgag
    39541 atccagagct ccaacaccga actcatgaac gccatcctgg cgggctatca cggagctctc
    39601 gcgttcgggg ggccgaatga cttcaccctc gtcatcgagt gacgccccgc cccaggcggg
    39661 gtccggcggt cccgggcgcc tcttccatat caaggcggcc ttgggaggcg ccgggctcct
    39721 tctgggactc gtgggcatga gtctccatcg cacgagcctc gtctggggcg ccgtcggctg
    39781 tctcggcgtc gcattcgtcc ttcgcttcgt gaaacctcga tgatcttcga tcctggcgcc
    39841 gttctcctcg tctcctgcta cgagctgggt caccagccat tggcgacgtc gcaggcggcg
    39901 gcgtttctgc agcgcgccgg attgcaaccc acccagctcg atttggcgca ggaggtgctg
    39961 acgccggccg ccgctcgtgg cgcggaagtc atcgtgttct ccgtgccgat gcacaccgcg
    40021 ctccgggtcg gtgttgaagc ggcgaccttg gcgaaaaggc tcaaccccgg agtgcggatc
    40081 ggattcttcg ggcactatgc gtggctgaat cggcagtacc tgctggatgg cgtcgcggac
    40141 tgggtgctgg cgggggaagg tgaggaggcg ctcgtcgagc gcctgtccac cggacgcatc
    40201 caaaccgatg ctcccgtcct gcggcgcctg gattttcccg tcccgtaccg cgagggcctg
    40261 ccgtcgctcg accattacgt catgatggag catggtgagc gacgcgtcgc ggcggggtac
    40321 gtcgaaggaa gccgcggatg tctccacctc tgccggcatt gtcccatccc gccggtctac
    40381 gggggacggt tcttcgccat cccagtggaa acggtgctcg ccgacattcg ggcgctggtc
    40441 gccgccggcg ccgggcacat cacgttcggc gatcccgact ttctgaatgg gcccgggcac
    40501 gcgcgaaagc tggctcaggc attgcaccgt gaattcccgg aggtgacctt cgatttcacc
    40561 gcgaaggtgg agcacctcct gaaacatcgg gcgctcttgc ccgagctcgt tcaggccggc
    40621 gcgttgttcg tcgtttcggc ggtcgaatcc ctgagcgaca ccgtgctcgg gcatctcgag
    40681 aaggggcaca cgcgctccga cgtgttcgaa gccctgcgtc tcacccggga agtcggtctt
    40741 gcgttgcggc cgtcgttggt ggcgtttacg ccgtggacga cgttggacga ctacgtggag
    40801 ttggtcgagt tcgtcggcga tcaggatctg acccggcaca ttgatccgat tcagctcacg
    40861 atccgcttgc tcattccccc cggctccgga ttgctggaac gggacgcggt tcgcccgttc
    40921 ctggggccgc tcgagcccgc ccggctcggg tacagctgga cccacccgga cccccggatg
    40981 gacgcgctct acacggtcgt gacgaaagtg atggagcagg gcaacaatga gggcgccgat
    41041 cccgaagtca ttgtggaaag gatcagggct caggcggtca cgattcgtga cggaagaccc
    41101 gcatctccgc gccccacaac gccgagcgtc actccggtgc cccgcctgac cgaggcatgg
    41161 ttctgctgcg ccgagcccgc gcccgagcag ctcgtcacgc tcaggtaacg gaaatagggg
    41221 cgtttttcca ccctgattca cactaggtcc aaagcctgta gttttcccac cacatggacg
    41281 cgtacacatt ctccctcgcc ctgggaggca tcggtcttct cgtcatgggt gtctccggac
    41341 tggggcaccg catgcatccc ggcggccacg ccgcgcacgg acatggtcac cacgggatgg
    41401 tgaaggctgg aggcaaggcc caggccgccc atcatggcac gcaacaggcg catgggatca
    41461 acgtcggcat cgcgcgcgtg tcgcgggcct ttctcacgat tctttcgccg cgcgtcgcgt
    41521 tcagcctcgt cctgggattc ggcgctacgg ggttcttgct gaagggcatt ttcggcggcg
    41581 cgatccttgt cgcgttcgca gccgtcgggg gagtggcgtt tgaaggcatt ctggtccgtc
    41641 cgctcttcaa cttcctctcc aagttcgcct ccgatcccgc gctcacgctc gacgcgacgt
    41701 tgggcgacga agtgcaggtc gtcacgggct tcgacacgca ggggcaaggg ctagtgtcgg
    41761 tcgagctgaa cggtcagatt gttcagttgc ttggcacgct cacgccggag gatcgctccg
    41821 gccccagagt gcgcgcgggg gatacgcttt tcgtacgaga tgtcgacagt gctcgtcacc
    41881 gagtggtcgt cgggagaact ccagactgac gtctcgtgtc tctttccagg agcggaaatg
    41941 cagaactgga tggtgatcgg cggggtggtg gtgggcgtgc tggtggtcat cccgctcatc
    42001 gtatccacgt ttctccggga cgtcgcggcc ggcgagatca ggctggtgag ctggattgcc
    42061 gggggcacgg tcacgtaccg cggcccgggc aagtccaagg aaattcctct gctcacgacc
    42121 gggaccacgc tgtcgtcgaa ggtcatcaat gtcgacctcg acatcaccga ccagacggcg
    42181 gacctggaca caagcgggac gccgcgcccc atcaaggtgc gcgtgctcgc cagcgctatc
    42241 gtgtcggtcg gggattcgga cgtgctgatc aagacggccg ccaatcgctt tttctccaag
    42301 tcggatggcg atcaggtgag cacgctcacg gacttgctgt cgagctctgg ccgccgcgcc
    42361 atcaatctgc tcactcatga ccagcttttt tccgccaaga ccgccggccg ccccgaaggc
    42421 gccaatctgc cggcgccgat cgcgacgggc accgacctgg tcgcggccgg ggcggtggaa
    42481 gacgatgacg atcccctgtc ggtgatcatc cggaaggcct gctcccggga gctgacggac
    42541 ctcggcctga tcttcaattc tctcaacatc aaggtcgtcc agtccgaggt cgccgacgcg
    42601 cggcgccggc aatcggcggc cgaggcacag gcgaatgcgg acatcgtctc ggcgcagcag
    42661 ggtcgccggg ccaaggaggc gcagctcgag gctgagcggg cgatttcgga caagcagcgg
    42721 gagctggagc agaccaaggc atcgaacgcg gccctgatcg cgcaggcgac cgcgaagatg
    42781 caggaagcgc tgggcgtaca gcgggaagcc gagctcgacg cgacccagat cgcgcaagcc
    42841 aaggcggacg ccgaaaagat gcggctggga gcggccgccg cggcggatgc ggaggccatc
    42901 cggatcacca agattgccga ggcgcagtcc gagagcatca agaaggtgaa tgaggcgatc
    42961 aaggccggtg gcgagagcta tttccggtat cgccagatcg aaatgctgcc ccagatcgct
    43021 ccggtgatcg ccgatgcgct cgcccaggcg aagttggtga ccatttctgg gggtgaaggg
    43081 ggtggggcgg cgggcacggc gaccaataac gtcgcctcgg tcatccagac ggtgctcgcg
    43141 gcgcagctcg tgacgaaggg catactgagc ccggaatcgg atggagggaa caatacccct
    43201 gccgctccgg ttacaaccgc aaagaacagg tgacaacgat gagcaatctg cacgttccga
    43261 tgtacgatcc gaatctcgtt cagccgatgc gcgaagagct cgtgcgcctc ggttttgaag
    43321 aactgcggac tcccgatcgg gtggatgagg tgatggcaaa ggccgccgac accacgctcg
    43381 tggtcgtgaa ttcggtgtgc ggctgtgcct ccgggcgggc gcgtcccgcc accgccatgg
    43441 cgttgcgtgg cgccaggaag cccagccgcc tcctcacggt gttcgccggt caggatgccg
    43501 aggcgaccgc gcgggcccgg gagtacttcg cgccctatcc gccttcgtct ccccagatcg
    43561 cgctcttcaa ggatggaaaa ctggtcttca tgctcgagcg gaagaacatc gagggccgga
    43621 ccccctcgga catcgcggcg gatctgagcg gtgccttcga ccggttctgc gcctgatgga
    43681 tgacggggcg aagaagctca tcgtcgtcgg agaccgggtg ctggtccttc cgcaggaagg
    43741 agaggaacgg accaacgtcg ggctctatct tcccgccacg gcgctcgagg gccggcaggt
    43801 tcagggcggg cggatcgtgg cgacgggccc ggggtcaccc atggccgagc cggcgtcgat
    43861 ggacgacgag ccgtggaaaa tccggtccag cgacgtgaaa tacctgccga tgcaggcgcg
    43921 cgtgagcgac tacgcgctgt tcttccgcaa agcggcggtc gagatcacgt tcgacgggaa
    43981 gacctacctc gtcgtaccgc aggcggccat cctcgtcctg gtacgggacg gcgtccccct
    44041 ggacgatcgc gcgtaattcg gacccgtagc ccggtgaaaa gaccccgctc caaccggcgc
    44101 ggggtctttt cttggcccat gacttcgttt cccgggggac tggagcgggc ccgcaggctc
    44161 ctggctgctc actggggata tggcggcttc cggccgaacc aggttcgcgt gctggctcct
    44221 ctcctcggcg gccgcgacgt cctggcgatt ctgccgaccg gagcggggaa gtccctttgt
    44281 ttccagatcc ccgccttgat gtcgagtggg ctgaccgtcg tcgtgtcgcc cctggtgtcg
    44341 ctgatgcagg atcaggtggg cgcgctgcgg cgccggggcg tcgcggcggc ctacatcaat
    44401 tccacgcaga cgctcgagca ccggcacgcg gtcctggacg ccgtgcgcgc aggcctggtg
    44461 cgtctcctct actgcgcccc cgagcgcctg ccctccctga ctcgccgcct cgcggcgtgg
    44521 actctgcgtc cggcactcct cgcggtggac gaagcacact gcatcacggc gtggggacat
    44581 gagttccgtc ccgcgtacca actcctcggg aagtaccggt atctgatggg caacccgccg
    44641 acgatcgcgg tcaccggcag cgcgaccacg cttgcgcgcg ccgagatcgc ccgggtgctc
    44701 cggctcgagg gggtcgtcga ggtcgtcggt tcattcgacc ggccgaatct ccgtttcacg
    44761 acggagctgg ttcaggatga ccgcgggcga ttcgcgaaga tcgtcgagcg agtgcggaga
    44821 gccaccgcgg ccaccatcgt ctacgcgccg acgcggcgcc tgacggagct gatcacccgc
    44881 gcgttgttgc tcagaagtct cagggccgcg ccgtatcacg cggggctgac ggcggcggtg
    44941 cgccgaagta cgctgaaggc cttcttgtcc gatcaggtcg gaacggtggt ggcgaccacg
    45001 gcgttcggga tgggcatcga caagcccgac gtacgctccg ttctgcactg gggacccgcg
    45061 cacaccctcg acggctacta tcaggaggcg gggcgcgccg ggcgcgacgg ccattccgcc
    45121 gaatgtcgag tgttctggag ggacggggac ctgcggtggc cggggatcgc accggccatg
    45181 aaggtctatc tgcaacgccg ggcctgtcgc aggcgtttgc tgctgcaata ctttggtgag
    45241 cggttggagc ggtgcgcggg atgcgactac tgcgacatcc ggtagatccg tcccgaagcc
    45301 atgaccacgt acacctcgcc cgacgcatcc tccccgaacg agaccacgtt gtcgttgatc
    45361 agaggagcct cgagcggcgt gaacgcgtcg accggtttgc cggcgaccat cttcagaccg
    45421 cgtacccacc cggcgcagta gtcaccgtag aggtaaaccc ccgacaggtc cggtgacgcc
    45481 ttcccgcggt agacgtagcc cccggtgacc gcgcacgccg gattgtgcgg gtacttgaag
    45541 agggggagga cgagtccggc cgtgtcgcac cccgtgcgat agcagacgtc gccctccatg
    45601 aacggccacc cgaagttgtt gccgggccgg gcgccaccgg ggaggtaatt gatctcctcc
    45661 cagaggttct ggccgacatc tccgatccac acgtcgcccg tctggcggtc gaaactccac
    45721 cgccagggat tgcggagtcc gagctgccag atctcatgca gccatccgga tcgcgcgacg
    45781 tacgggttgt cggccggaat gccgtacgga tctcccgcgt ccacgtccac acgcaggagt
    45841 ttcccctgat acgccatgct gtcctgggag cggctcgtct gcgcggtgtc gacgttggcg
    45901 tccccatcgc cgatggcgat gtagaacttt ccgtcggggc caaaggtgac gaggccgcca
    45961 tatagcaccg cgtccgccgc ctgggggacg ttcaggatcg agtgttcggt ggcggggtcg
    46021 gcgtgatcga acgtcgaatc ggcggtgaac cggctgacct gcgccgcccc ggcattatcc
    46081 acatagaaga catagacgta ccggttggtc gcgtagtgcg ggtgaaaggc gatgctgtag
    46141 acgccgtatt ccaaggctcc gccggtgagc gccgtcaagt tcagaaatgc agagtcctgc
    46201 cgaacgccgt gcttgcggat caggatccgg cccccgcgct ccaccacgaa gagccggctg
    46261 gtgtcgccgg gcggggccgc gacgaacacc gagaagtcgt agccggaatc gaccgggatg
    46321 agcaccgggc cgccggcccg ggtaatgacg gtcgttgcgg aatcgtgaca ccctgatgcg
    46381 agggcgatgg ccaggaggag ggcggggagg gggacccgga gtttcacggc caaaaggtag
    46441 cccccctgcc tatatttcag aatgcacgac gaactgtact tctccgagca gcacctccag
    46501 gtccgtgaaa tggtgcggga tttcgcccgc aatgtggtcc gtcctagcgc gcgggcttac
    46561 gacctggact cgaagttccc ctgggacaac gtcaagcaga tggcggatct gggcctcttc
    46621 ggcatcccgt ggcctgagga gctgggtggt tcagggatgg actacttgtc gtacatgatc
    46681 gtgatccatg agatggccaa ggtggacgcg agccacgcca tcaccatctc cgcccacacc
    46741 accctcggca cctcgccgat cgtcgatttc gggaccccgg agcagaagaa gaagtacgtc
    46801 ccgctccttg ccaccggaaa ggtgctgggc gggttcgggc tgaccgaacc tggtgccggc
    46861 tccgacgcgg ggggcaccaa gacggtcgcg gtggagaagg gcgatcacta cgtcctgaac
    46921 ggctcgaaga tcttcatcac gcatgccggc gtcggtgaga tcttcgtggc gacggcgcgg
    46981 accgatccat ccgccaagaa gacccatggc atcaccgcct tcatcgtcac gaaggacacg
    47041 acggatctcg ccgaggcgac ccgggtgggg atcggacacg aggcgtcgtt ggccaagatc
    47101 cccggattct cggcggggaa gaaagaggac aagctcggct ggcgggcgtc ggacacccgg
    47161 gagctcgtgt tccaggacgc catcgtcccg aaggagaacg tcctcgggcc cgtgaacaac
    47221 gggttccgcc agttcctgca caccctcgat ggtggacgga tcgggatcgc ggcgctctct
    47281 ctcgggatcg cggagggcgc gttcgaggag tgcctggcgt acacgtcgac gcggaaacag
    47341 ttcggaaagc ccatcgcgag cttccagggg gtccacttcg cactcgcgga catggccacc
    47401 gagatcgagt gcgccaagca cctcgtcttc cacgcggcat ggctcaagca gcatggccat
    47461 ccgttcaagc gcgaagccgc catggccaaa ctgtacgcct cggagctcgc catgcgcgcc
    47521 acgacgaagg cggttcagct ccacggcggg tacggctaca cgacggagta tccggtggag
    47581 cggatgatgc gcgacgccaa gatctgcgaa atcggggaag gcacgagcga gatccagcgg
    47641 atcgtgattg ccaggcagtt cttgggggat ttgatcgact gaggcgaacg cggaacgggg
    47701 agcgcggaag ggggaaccgg ccggatggtt ccgcgttccg agttccgcct tccgcgttct
    47761 tcagagttcc ggcggcttgt acggcccagg ctcgaccgtt ttctcctcgg ggtgcgacag
    47821 ttccttgtgg agatgctcga gcagctcgag ctgccgctgc tgcaaatgcc agagctcatg
    47881 ccactgcttg taccgaagtg agtccaactt gtcgtgcagg tcccggattt caatctccgc
    47941 catcaagttg atctcgtagt cctgcttggc ctgcagccgg tccttctccg cctgccggtt
    48001 ctggctcatc atgatcacgg gagcctgcag ggcggcaagc gtcgacag
//
LOCUS       DASUBP010000007         3902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_422561, whole genome shotgun sequence.
ACCESSION   DASUBP010000007 DASUBP010000000
VERSION     DASUBP010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3902
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_422561"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..893)
                     /locus_tag="VFU75_00380"
     CDS             complement(<1..893)
                     /locus_tag="VFU75_00380"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5465312.1"
                     /translation="MSNAVEVRDILYRAGKAFEIRDLSLNVPTGSVYGFLGPNGSGKT
                     TTIRLLLGLLRPARGRITVLGGDVPKDAPRVLARTGYVPESPHLYPMLTVEQLIRVHA
                     SFYPQWDGAWSRDLQRTFRLDGSTRFSSLSKGEKGKLMMLLALAQRPELLLLDEPTDG
                     LDPVVRRDVLSALLDYVSQQNATVFISSHLIHELERICDWVGVMDRGHLVAELPMQSF
                     KSGIKRLRVSNPPPRLGDTPFVILSREPGTGSAETWVVRGWQPPMTQYFEGVGAGLKE
                     VVDLDLEEGFVELLNTFRQPQ"
     gene            complement(890..1270)
                     /locus_tag="VFU75_00385"
     CDS             complement(890..1270)
                     /locus_tag="VFU75_00385"
                     /inference="COORDINATES: protein motif:HMM:NF012610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU5465313.1"
                     /translation="MFEQIDPRSATPLYAQIAGRLRVAVAAGELRPGEPLPSVRQLAS
                     KLRVNPATVVQAYRDLETEGFVEMRQGAGTFVKEVVPERKATERSKQAIDLVRQMIAE
                     AGRLGLTARELQQAIEQELEARIG"
     gene            1347..2534
                     /locus_tag="VFU75_00390"
     CDS             1347..2534
                     /locus_tag="VFU75_00390"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEU5465314.1"
                     /translation="MSQRILVINAGSSSLRVALFQKASREAVLTAMVERIGAGEALLR
                     IDRAGAATRTEPLAAKDHTEALAALLSRLEREDLRREDVVAVGHRVVHGGDKFSEPVV
                     ITPAVIAEIGRASDLAPLHNPYNLLGIEAAKSAFPSAPHVAVFDTAFHSTLPPEAHLY
                     AIPYQLYRHHRIRRYGFHGTSHGYIRHHLPRVLKRKLPARVVSLHLGNGASACAILNG
                     ESVDTTMGLTPLEGLMMGTRSGDIDPAVIGHLVGREEMSVGDALALLNQQSGLRGVSG
                     LSSDVRDLLKAESEGDERAKLALNLYCYRIGKTIGAMAAVMGGVDAVAFTAGVGENAV
                     ALRARSLKRLGHLGIRVNPAKNRKLVGGVEGVFHGGRVALAVVHTDEARVIAVATAKT
                     TGR"
     gene            2531..3424
                     /locus_tag="VFU75_00395"
     CDS             2531..3424
                     /locus_tag="VFU75_00395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HEU5465315.1"
                     /translation="MNILVSGGSGFVGTALIAGLRADGHRVRVLTRHRDAAGPETPFW
                     NPEAGELDRAAFRDVDACINLSGSTIATRWNAEKKKEIRESRVRCTTLLAETMASLEA
                     RPGVFISASATGYYGDRGGEVITESASAGRDFLADVCVAWEAAATPAERAGVRVVHPR
                     LGLVLSPTGGVLKQALPPFRAGIGGPLGSGKQWWSWVVLHDVVGAIRHALTADGLRGP
                     VNVVAPGAVPNAEFAKTLGLVLGRPSAMPVPAMALKLLFGEAAEGAILTGVHAVPEAL
                     EKTGFTFAHPELEGALRHLLT"
     gene            3622..>3902
                     /locus_tag="VFU75_00400"
     CDS             3622..>3902
                     /locus_tag="VFU75_00400"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU5465316.1"
                     /translation="MAGTTATDESGKIVGPGDPYRQTVQVLKNIERALIKAGGSLNDV
                     VRTRMFVTDIKYWQDVGRAHGEVFGKIRPAATMVAVTALVDPDMLVEIEA"
BASE COUNT          655 a         1247 c         1331 g          669 t
ORIGIN      
        1 tgctgcggtt gacggaaggt gttcagcagc tccacgaacc cttcctcgag atccaggtcc
       61 accacttcct tcagtcccgc gcccacccct tcgaagtact gagtcatcgg cggctgccac
      121 ccacgcacca cccacgtctc ggccgatccg gtgccgggct cgcgggagag aatgacgaaa
      181 ggcgtgtcgc cgaggcgggg tgggggattg ctgacgcgga ggcgtttgat gccgctcttg
      241 aagctctgca tcggcagctc ggcaacgagg tggccgcgat ccatgacgcc cacccagtcg
      301 cagattctct ccagctcgtg aatcaggtga ctggagatga agaccgtcgc gttctgctgg
      361 gatacgtagt ccagcaaagc ggagagcacg tcacgacgaa ccacgggatc gagcccgtcg
      421 gtgggttcat cgagcagcaa gagctccgga cgctgtgcga gcgcgagcag catcatcagc
      481 ttccccttct cgcctttgga gagggagctg aagcgtgtgc tgccatcgag gcggaaggtc
      541 cgttgcaggt cccggctcca ggcgccatcc cactgcggat agaacgacgc gtggacgcga
      601 atcaactgct ccacggtgag catggggtac agatgcgggc tctcagggac gtacccggtc
      661 cgggccagca cgcgcggcgc gtccttcgga acgtcgccgc ccaggacggt gatgcgtccc
      721 cgagcgggcc ggaggaggcc gaggaggagc cggatcgtgg tggtcttccc cgagccgttg
      781 gggcccagaa atccgtagac cgatccggtg ggcacgttga gtgacaggtc acggatctcg
      841 aatgccttcc ccgcgcggta gaggatgtcc cgcacttcga cggcgttgct catccgatcc
      901 tcgcttccag ttcctgttcg atcgcctgct ggagctcacg tgcggtcaac ccgagccgcc
      961 ccgcctcggc aatcatctgc cgcacgaggt cgatcgcctg tttgctccgt tcggtggcct
     1021 tgcgttcggg aacgacttcc ttgacgaagg tccccgcgcc ctgtcgcatc tccacgaacc
     1081 cctccgtttc gaggtcccgg tacgcttgca ccaccgtcgc gggattgacc cggagcttcg
     1141 acgcgagttg acggaccgac ggaagtggct ccccgggacg cagctcgccg gcggccaccg
     1201 cgacgcggag gcgtccggcg atttgggcgt agaggggggt ggcacttcgg gggtcgatct
     1261 gctcaaacac gcgtgccgat cactgtattg gtgttccaat acagtgtgac aatgaggtcg
     1321 tcccgtcaag ggtaggttat aagcccatgt ctcagcgcat cttggtcatc aacgccggct
     1381 cgtcgagcct ccgagtagcc ctttttcaga aggcctcaag ggaggcggtg ctgaccgcga
     1441 tggtcgagcg cattggggcg ggtgaagcgt tgctccgcat cgaccgtgcg ggcgcggcga
     1501 cccgcaccga accgctcgcc gccaaagacc atactgaagc gctcgccgcc ctcctcagcc
     1561 ggctggagcg ggaagacctg cgtcgcgaag acgtagtggc cgtggggcac cgggtggtcc
     1621 acgggggcga caagttcagc gagccggtcg tcatcacccc cgcggtgatc gccgaaatcg
     1681 gccgcgcgtc agacctggcg cccctccaca atccctacaa cctgctcggc atcgaggccg
     1741 ccaagtccgc gttcccgtcg gcgccgcacg tcgcggtgtt cgacaccgcg tttcactcga
     1801 ccttgccccc ggaagcgcac ctctacgcga ttccctatca actgtatcgg caccaccgca
     1861 tcaggcgata cggctttcac ggcacgagcc atggatacat ccggcatcac ttgccgcgcg
     1921 tactgaaacg gaagctcccg gcgcgcgtgg tgtctctgca cctcggcaac ggcgcgagcg
     1981 cgtgcgccat cctgaacgga gagtcggtgg acaccacgat gggcctcacg cccctcgaag
     2041 gcctgatgat gggcacccgg tcgggggaca tcgacccggc cgtgatcgga catctcgtgg
     2101 gccgcgaaga aatgagtgtg ggggatgcgc tcgccctctt gaaccaacag agcggtctca
     2161 ggggcgtgag tggtctttcg agcgacgtgc gggatctgct caaggcggaa tcggagggcg
     2221 atgaacgcgc gaagctggcg ctgaacctct actgctaccg gatcggcaag accatcggcg
     2281 cgatggcggc cgtgatgggt ggcgtcgacg ccgtagcgtt taccgcgggg gtcggggaga
     2341 acgcggtggc cctccgcgca cgttctctga aacgcctggg ccacctgggc attcgtgtca
     2401 atccggcgaa gaatcggaag ctcgtgggcg gcgtcgaggg ggtgttccat ggcggtcggg
     2461 tagcgctcgc cgttgtacac acggacgagg cccgcgtcat cgccgtcgcc actgccaaga
     2521 ccaccggccg atgaacattc tggtctcggg aggatccggg ttcgtcggca ccgcgctgat
     2581 cgccggcctg cgggcagacg ggcatcgggt tcgagtgctg acacgtcacc gggacgccgc
     2641 cggcccagaa acccccttct ggaatccgga agcgggcgag ctggaccggg ctgcgttccg
     2701 cgatgtcgac gcgtgcatca atctttccgg ctcgaccatc gccacccgct ggaatgcgga
     2761 aaagaagaag gagatccggg agagccgggt ccggtgcacc acccttctgg cggaaacgat
     2821 ggcgtcactc gaggcacgcc cgggcgtctt catctcggcc tctgcgaccg ggtactacgg
     2881 tgaccgcggg ggcgaggtga tcaccgaatc cgcgtcggcg gggcgtgact ttctcgccga
     2941 cgtctgcgtg gcctgggaag ccgccgccac tcccgccgaa cgcgccggcg tgcgggtcgt
     3001 ccacccccgg ttgggtctgg tgctctctcc cacgggtgga gtgctgaagc aggcgttgcc
     3061 gccattccgc gccggcatcg gtggaccgct gggaagcggg aagcagtggt ggagttgggt
     3121 ggtgctgcac gacgtggtcg gcgccatccg gcacgccctg acagccgacg gccttcgggg
     3181 accggtgaac gtggtggcgc ctggagcggt gcccaatgcg gagttcgcca agactctcgg
     3241 gctcgtgctc ggacgcccgt cggccatgcc ggtcccggcg atggccctca agctgctctt
     3301 cggcgaggcg gccgagggcg cgatcctcac cggtgtgcat gcggtgcctg aggctctgga
     3361 gaagacggga ttcacgtttg cgcacccgga gttggagggg gcgttgaggc acctgttgac
     3421 gtgacatgaa cgcggagcgc ggaaggggga acgcggaacc ggtccccccg attctgctcc
     3481 aagttccgca ttccgcgttc cgcattccga cttcgattcc tgaggggacc aatgatgggt
     3541 cgtcagaata tctcatcggg aacaccgtgg gaaccaagag tcggttattc ccgggccgtc
     3601 cgagtgggag acttcatcca tgtggcgggc acgacggcca ccgacgagtc ggggaagatc
     3661 gtgggtcccg gcgatcccta ccggcagacg gtgcaggtgc tcaagaacat cgagcgcgcc
     3721 ttgatcaagg cgggcggttc actgaacgac gtggtccgca cccggatgtt cgtcaccgac
     3781 atcaagtatt ggcaggacgt gggccgtgcc cacggggaag tgttcgggaa gatcaggccc
     3841 gctgccacca tggtcgcggt caccgccctc gtggatcccg acatgctggt cgagatcgag
     3901 gc
//
LOCUS       DASUBP010000008         9440 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_477161, whole genome shotgun sequence.
ACCESSION   DASUBP010000008 DASUBP010000000
VERSION     DASUBP010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9440)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9440)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9440
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_477161"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VFU75_00405"
     CDS             complement(<1..569)
                     /locus_tag="VFU75_00405"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5465317.1"
                     /translation="MSEQFLSSDEYDERAHQLYNEGQYDEAVDVLREGLSLYPHAVEL
                     HVGMGYARLAREEFVWARRSFEEAVGLDSDHEDALAGLGEVLLKFGERDAAAACFDRI
                     LELGFRDDHDLMLQIGRALFREGVLEQARRYFEVAITGHPDSAEAAACVGYAAHRLTD
                     EGGALHWLRRALELDPSHGEARIYLANLL"
     gene            656..1888
                     /gene="argJ"
                     /locus_tag="VFU75_00410"
     CDS             656..1888
                     /gene="argJ"
                     /locus_tag="VFU75_00410"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: protein motif:HMM:TIGR00120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004358 - glutamate N-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HEU5465318.1"
                     /translation="MSNEPEFLPEGSVTTTPGFTAAAAHAGLKAGGALDVALLVSSSF
                     CATAGVFTTNAVRAAPVLYDEAMLAERPGRVRAVAMNARVANACTGAPGLEAARAMAR
                     AAEQAAGLPARSALVLSTGVIGVPLPVDKITQGLTDAAGRLAQSGGLDAAKAIMTTDT
                     RAKHCAARIATPSGSITVGGIAKGAGMIHPDMATLLGVLTTDAKIEPAELAPILRRVA
                     DRSFNAISVDGDTSTNDTVLILANGASRIEPTHDPGLLQLFESAVLLVARSLALAIVV
                     DGEGATKLVEIQVRGAKNETAARDVGRAIARSALVKTAIFGADPNWGRILAAAGAAGV
                     ELAPEKLSLSAEDDGGWLDLARGGGTANPDLARARAIFQAQRICLRIDLGMGRAEAVV
                     WTCDLSPEYVRINADYTS"
     gene            complement(1896..2546)
                     /locus_tag="VFU75_00415"
     CDS             complement(1896..2546)
                     /locus_tag="VFU75_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465319.1"
                     /translation="MNPSLLLVLLLLPQQTDGSRLTGHVPAAALPGVQAIITRAAQDS
                     LPTEPLVEKALEGGAKGAPPDMIVTAVGAEEARLRTARDLLRGAGAPGPYATSEIQSV
                     SLALARGLPDRIVSKVVGAVRDTAPGFALHAVADLSAHGFSQDSSASLIVAALKTGLA
                     GHRLLDVAAAADREVQRGSTRMQALAKVRAILPAIPSQRVPPGAMQGATRPKKGGG"
     gene            complement(2543..3154)
                     /locus_tag="VFU75_00420"
     CDS             complement(2543..3154)
                     /locus_tag="VFU75_00420"
                     /inference="COORDINATES: protein motif:HMM:NF027875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HEU5465320.1"
                     /translation="MTHNLDLRIHRALDGELDRASLSPEELALVERLDAAARALSDVP
                     PVPGLAHRVLGIIRRPLESPLERFRRWVTSPHPVTVSLRPVYSVIIAVLFVALLWRSP
                     ASGPLLADHEGVAQFVARFPEAHSVAVVGPFNDWRPEATPLSDDDHDGVWDARVILPA
                     GPHEYMFVVDGERWVADPMAGKYVDDGFGRQNAVMEVQPVRSE"
     gene            complement(3151..3699)
                     /locus_tag="VFU75_00425"
     CDS             complement(3151..3699)
                     /locus_tag="VFU75_00425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5465321.1"
                     /translation="MTAGEGPSDAEVIARVLSGDVEAFGVLITRYEGGLSRFATRMLG
                     SRDAAADAVAEGLVRAYRHLASCRDPARFRTWLYRIVANRCKSHLARRPVGDVSLDDA
                     PPMAAQELADAQFERTDQLERLERALAMLAPEKREAFVLKHLDGMSYEEMSVVTGERI
                     PTLKMRVHRAREALLAALKESP"
     gene            complement(3819..5333)
                     /locus_tag="VFU75_00430"
     CDS             complement(3819..5333)
                     /locus_tag="VFU75_00430"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF255 domain-containing protein"
                     /protein_id="HEU5465322.1"
                     /translation="MALLAVSAVSAVACATERGPLTNRMAQTETRYLARAARQPVGWQ
                     PWGRDAFTLAARLDRPILLYIGADDCRWCLVMDREAYADKDLGAMIDSLFVPVRVDRD
                     ERPDVARRYEAEVGVIAGLHGVPLTVVLTPDGAAFFGGTYFTPDDPVTGRGLKQILPA
                     VAKSYHTERATVVQRAALVRQVAENRRNTRGVLHAQAVDSGVNAVRAAVARPGAVTGF
                     VATQAINLLLSEYGSRGDTASLSVGREALNAMLDSGAVGADGTQDDPPVTVRAGLARD
                     LALAWVLTADPRYQTAARRETRILAHQLLEPTDHTVFTDREAYAIGSLLEAASAVGDT
                     GAQGAARAALDALLRQVYASGYGVRHTLVGSVDGLLQDQLQVAVASLDAYEATGMARY
                     LDVAKDLSNLMERSYGDPLGGFYDATPESAPIPALADRTESALDDLLPAANPWAAAFL
                     LRLSDVIGDSRLRRRARATLEAVAGAVSGGGPRASGFLAAAREVLAPPSAVPAH"
     gene            5551..5730
                     /locus_tag="VFU75_00435"
     CDS             5551..5730
                     /locus_tag="VFU75_00435"
                     /inference="COORDINATES: protein motif:HMM:NF039193.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HEU5465323.1"
                     /translation="MAKAGKAPMAADEGYCVKCKAKRQISNAQQVKMANGRPAMKGVC
                     PVCGTGMFKILPPQK"
     gene            complement(5806..7434)
                     /gene="pruA"
                     /locus_tag="VFU75_00440"
     CDS             complement(5806..7434)
                     /gene="pruA"
                     /locus_tag="VFU75_00440"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HEU5465324.1"
                     /translation="MNSIPKIPTPVNEPVLSYAPGTPERATLKGALAELSGQTMEIPL
                     IIGGKEVRTGKSIPVVAPHRHKQVLANVHQAGPAEVQAAIKAAREAWQEWSRWSLADR
                     SAVFLKAADLLAGSRRAFANATTMLGQSKTAHQAEIDAACELIDFFRYNVHYAERIVG
                     EQPMSPAGTWNRLDHRPLEGFVYAITPFNFTSIGGNLPTSPAIMGNTVVWKPAATAAL
                     SNYAIMKILLEAGLPPGVINFLPGPSGQISDLLLADRHLGGIHFTGSTEVFQMLWKRV
                     SDHLPNYADYPRLVGETGGKDFVLAHASADPDALAAGLIRGAFEYQGQKCSAASRAYV
                     PDNIWPRVKERMGDMLSSLKMGDPADFSNFVGAVIDRKAFDKIKGYIDGARKGNGAKV
                     LFGGECDDREGYFVRPTVLQADDPKYRTMCEEIFGPVLTLHVYPAAKWKDTLGLVDGT
                     SPYALTGAVFAQDRGAVREAETALRYAAGNFYINDKPTGAVVGQQPFGGARASGTNDK
                     AGSLLNLVRWVSPRAIKENFVPPKDYPYPFMQQS"
     gene            7601..8077
                     /locus_tag="VFU75_00445"
     CDS             7601..8077
                     /locus_tag="VFU75_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5465325.1"
                     /translation="MTIDPGTKIGHVHLTVANLDRALAFYRDVLGFEVTTRYGREAVF
                     LSAGGYHHHVALNTWAGEGAPAPAPGTTGLYHFAILYPDRKALARALKNVLDNGIVLE
                     GASDHGVSEAIYLRDPDGNGVELYRDRPQEEWPRAEDGSLRMHTRPLNLAQLLAEA"
     gene            complement(8082..>9440)
                     /locus_tag="VFU75_00450"
     CDS             complement(8082..>9440)
                     /locus_tag="VFU75_00450"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5465326.1"
                     /translation="GSPAARAANDWTMQRFKDYGLDAHLESWEFGGTWTRGPMWIRMT
                     APRKHDVIGASWAWTVGTGGKTVAGPVVLINANNQDSLDAYKSKVRGAWLMLREPANV
                     WNNDGPPMTTADSEAMRRRFGNGGRPQLDSAARAKAAQFRVDLPYMLRNLGAKGILVD
                     GAKEQTLLTMSGSPNAPLPLPQVAIAHEDYTLFDRLIKQGITPRIEANIQSSMSMKPV
                     SQWNTVAEIKGSEHPGQVVIVGGHLDSWDLGTGATDNGTGSIATLEAARIIAQSGVKP
                     KRTIRFVLFTGEEEGLLGSQAYAKAHAGEADSIQAVIVLDNGVGAVTGQALQGRAELA
                     GLWKSILAPVASLNADSVRDHNKGGTDHLSFLAYGVPGFNFDQLTRGYNHTHHSQSDT
                     YDKAIPWDLEQASTVMAVSAVELANLSGMIPRGTKQEVQNFFGPKPSPSVVHPAPVAG
                     KK"
BASE COUNT         1532 a         3296 c         3132 g         1480 t
ORIGIN      
        1 tagagcaggt tggccaggta gatgcgggct tcgccgtgcg acgggtcgag ctcgagcgcc
       61 cgccggagcc agtgcagagc gcccccttca tcggtgagcc ggtgcgccgc gtaccccacg
      121 cacgccgccg cctccgccga atcggggtga ccggtgatcg cgacttcgaa gtaccggcgg
      181 gcctgctcca gcacgccttc gcggaagagc gcccggccga tctgcagcat gaggtcgtga
      241 tcgtcccgga aaccgagctc gagaatccga tcgaagcacg cggccgcggc gtcgcgctcg
      301 ccgaacttga ggagcacttc ccccaggccc gccagcgcgt cctcgtggtc cgagtccagg
      361 cccaccgctt cttcgaagga ccgacgagcc cacacgaatt cttcgcgagc aaggcgcgcg
      421 taccccatac ccacgtggag ctccacggca tggggataga gcgacagccc ctcgcgaagc
      481 acgtctacgg cttcgtcgta ttgtccttca ttgtaaagct gatgagcacg ttcgtcgtat
      541 tcgtcggagc tgaggaactg ttccgacatc gaacggaacc cccgggatgg ttgcgctatc
      601 atacgtaacg cgctccggtt acacaagcgc ttgacccttc gtatcttgac cctccatgtc
      661 caatgagcct gagtttctcc ccgagggatc ggttaccacc acacccgggt tcaccgccgc
      721 cgccgcgcac gccggtctca aggcgggtgg cgcgctcgac gttgccctgc tcgtcagctc
      781 gagcttctgc gccactgcag gcgtcttcac caccaacgcg gtgcgcgccg cgcccgtcct
      841 gtacgacgaa gcgatgctcg cggagcgccc cgggcgggtt cgcgccgtcg cgatgaacgc
      901 gcgggtcgcc aacgcatgta ccggggcgcc cgggctcgaa gccgccaggg cgatggcgcg
      961 tgctgccgag caggcggccg gactgcccgc ccggagcgcc ttggtgctct cgaccggtgt
     1021 catcggcgtg cccctcccgg tggacaagat cacccagggc ctcaccgatg ccgcaggccg
     1081 cctcgcgcag tcgggcggac tggatgccgc gaaggccatc atgaccaccg acacccgcgc
     1141 caagcactgc gccgcccgca tcgccacccc ctcaggctcc atcacggttg gcgggatcgc
     1201 caagggggcc ggaatgatcc atcccgacat ggcgaccctg ctcggcgtcc tgaccaccga
     1261 cgccaagatc gagcccgcgg agcttgcgcc gatcctgcgc cgggtcgcgg accggtcctt
     1321 caacgccatt tccgtcgacg gcgacacgag cacgaacgac acggtgttga tcctggccaa
     1381 cggggcgtcc aggatcgagc cgacccacga ccccggactc ttgcagctct ttgaaagtgc
     1441 cgtcctgctc gtagcgcgga gcctcgcgct cgccatcgtg gtcgacggtg agggcgcgac
     1501 caagctcgtc gagattcagg tgcggggagc gaagaacgaa accgcggcac gcgacgtcgg
     1561 ccgggccatc gcccggtcgg cgctcgtcaa gaccgccatc ttcggcgcgg acccgaattg
     1621 ggggagaatc ctcgcggccg cgggcgcggc cggcgtggaa ctggcaccgg agaagctgtc
     1681 gctctcggcc gaggatgacg ggggctggct ggacctggcc cggggcggtg ggaccgccaa
     1741 ccccgatctc gcccgcgccc gcgccatctt ccaggcacaa cgaatctgcc tccgcatcga
     1801 cctcggaatg ggtcgcgcgg aggcagtggt ctggacctgc gatctgtcgc cggagtacgt
     1861 ccggatcaac gccgactata cgagctgacg gcctctcacc ccccgccctt cttcgggcgg
     1921 gtcgctccct gcatcgcccc cggcggcacc cgttgcgacg gaatcgccgg cagaatcgcc
     1981 cggaccttcg cgagcgcctg catccgcgta cttccgcgct gcacctcccg gtccgcggcg
     2041 gccgcgacgt ccagcaaccg gtgtcccgcc agccccgtct tgagcgccgc cacgatcagc
     2101 gacgccgacg aatcctggct gaatccgtgc gccgagagat cggccacggc gtgcagggcg
     2161 aatccgggcg ccgtgtcccg aaccgcaccc acgaccttcg agacgatccg gtcgggcagc
     2221 ccacgggcga gcgcgaggga aaccgactgt atttccgagg tggcgtaggg gccgggagcg
     2281 ccggcaccgc gcaggaggtc ccgcgccgtc cgcaaccgtg cctcttcggc gccgaccgcg
     2341 gtgacgatca tgtccggcgg agcccccttg gcgccgccct cgagcgcctt ctccaccagc
     2401 ggctccgtcg gcagtgaatc ctgagccgcc cgtgtgatga tcgcttgcac gcccgggagg
     2461 gccgccgcgg ggacgtgacc cgtcagcctc gacccgtcgg tctgctgggg gagcagcaac
     2521 aggacgagca gcagcgacgg gttcattcgg accgcaccgg ctggacttcc atcacggcgt
     2581 tctgacggcc gaatccgtcg tccacgtatt tgcccgccat cggatccgcc acccaccgct
     2641 caccgtcgac gacgaacatg tactcgtgcg gccccgcggg aagaatgacc cgcgcgtccc
     2701 agaccccgtc gtggtcatcg tcggaaagcg gcgtcgcttc ggggcgccag tcgttgaacg
     2761 gacccaccac cgccaccgag tgcgcctcgg ggaaccgcgc gacgaattgg gccacgcctt
     2821 catgatcggc gagcaagggg ccgcttgccg gagagcgcca caacaaggcg acgaacaaca
     2881 cggcgatgat cacgctgtat accggacgga gcgacacggt gaccgggtgc ggcgacgtga
     2941 cccaccggcg gaaccgctcg agcggggact ccagaggccg gcggatgatc cccaggaccc
     3001 gatgcgccag tcccgggacc ggcggcacgt ccgacagcgc ccgcgccgcc gcgtcgagcc
     3061 gctcgacgag cgcgagctcc tcgggcgaca gggaagcgcg gtccaactcg ccatccagtg
     3121 ctcggtgaat ccggagatcg aggttgtgtg tcatggagac tccttcagcg cagccaacag
     3181 ggcttcgcgg gcgcggtgca cccgcatctt caacgtgggg atccgctcgc ccgttaccac
     3241 cgacatctct tcgtaactca ttccgtccag gtgcttcagt acgaatgctt cccgcttctc
     3301 cggggccagc atcgccagcg cccgctccag ccgttccagc tgatcggtcc gttcgaactg
     3361 ggcgtcggcc agttcctgcg ccgccatggg tggcgcatcg tccagcgaaa cgtccccgac
     3421 gggccggcgc gccaagtgac tcttgcagcg gttcgccacg atccggtaca gccaggtccg
     3481 gaatcgcgcg gggtcccggc aactcgcgag atgccggtag gcccgcacca gcccttccgc
     3541 cacggcatcg gccgcggcgt cccgggagcc aagcatccgg gtggcaaacc gggacagacc
     3601 gccttcgtac cgggtgatga gcacgccgaa tgcctcgacg tcacccgaca agacccgggc
     3661 gatgacttcg gcatcggacg gcccttcacc tgctgtcaat cagacctctt gaaaagggag
     3721 ggagtcacgt tcgggcgacg ggacgtgggc aaacgtggcg ttctgagacg cccggcaccg
     3781 gcggcccggt gtgctcacgg acgaatatgc tgtggggtct agtgcgcggg aacggcggag
     3841 gggggggcga gcacctcgcg cgccgcggcg aggaagccgg aagccctcgg gccgcccccc
     3901 gacacggcac cggcgaccgc ctccagcgtg gcgcgcgccc gccggcgcag ccgggagtcg
     3961 ccgatgacgt cggacagccg gagcaggaag gccgcggccc acggattggc ggcggggagc
     4021 agatcgtcga gcgcgctctc ggtccggtcg gcgagtgccg ggatcggggc cgactccggc
     4081 gtcgcatcgt aaaagccgcc cagggggtcg ccgtagctgc gctccatgag gttggagaga
     4141 tccttggcga cgtccaggta ccgagccatg ccggtcgctt cgtaagcgtc gagcgacgcg
     4201 acggcgacct gcagctggtc ctggagcagt ccgtccacgg accccaccag ggtgtgccgc
     4261 acgccatacc cggaggcgta cacctgccgc aggagcgcgt cgagggccgc gcgcgccgct
     4321 ccctgtgcgc cggtgtcgcc gaccgcgctc gcggcttcga gcagcgagcc gatcgcgtac
     4381 gcctcgcggt cggtgaagac ggtgtggtcg gtcggttcga ggagctggtg cgccaggatc
     4441 ctggtctcgc gacgtgcggc ggtttgatac cggggatcgg cggtgaggac ccacgcgaga
     4501 gccaggtccc gggcgaggcc ggcgcgcacg gtcaccggcg ggtcgtcctg ggtcccatcg
     4561 gccccgaccg cccccgagtc gagcatcgca ttcaacgcct cacgcccgac gctgagcgac
     4621 gccgtatctc cccgcgatcc gtactccgag agcagaaggt tgatcgcctg cgtcgcgacg
     4681 aatcccgtca cggcgcccgg gcgcgccacc gccgcgcgca ccgcgttcac cccggagtcc
     4741 accgcctgcg cgtgcagcac gccgcgcgta ttccggcgat tctccgcaac ctgacgcacg
     4801 agcgcggcgc gctggacgac ggtggcgcgt tcggtgtggt aggacttggc gacggcggga
     4861 agaatctgct tgaggccccg gcccgtgacg gggtcgtcgg gtgtgaagta ggtgccgccg
     4921 aaaaacgcgg cgccgtccgg tgtgaggacc acggtcagcg gcacgccgtg caaacccgcg
     4981 atcaccccga cctcggcctc ataccggcgc gcgacgtcgg gacgctcatc acggtcgaca
     5041 cgtaccggaa cgaagagcga atcgatcatc gctcccaggt ccttgtccgc gtaggcctcg
     5101 cgatccatca ccaggcacca gcggcagtcg tcggcgccga tatagagaag gatcgggcga
     5161 tcgagccggg ccgcgagtgt gaacgcgtcg cgtccccacg gctgccagcc caccggttga
     5221 cgcgcggccc gggccaggta ccgggtctcc gtctgggcca tccggttggt cagcggtccc
     5281 cgctccgtcg cgcaggcgac ggccgagacg gccgagacgg ccagcagtgc caactgggca
     5341 tgacgtgagt ggggtcgcat cgtgaagggc cgcgcaaaga tacccgctcc aggcccgtcg
     5401 cgttccagca acactgtcag ggctgtcctc cctacggctc ccacggcgga acccgacggt
     5461 attatgttgc acccgaacgg gacgcccccc actaggggtg ctggtttccc ggctaccaga
     5521 acccccgact cccaacggag ggaagcatca atggcgaagg ctggaaaggc cccgatggcc
     5581 gcggatgagg gctattgtgt gaagtgcaaa gccaagcgtc agatctcgaa tgcgcagcag
     5641 gtgaagatgg cgaacggccg tcccgcgatg aagggcgtat gccccgtctg cggcacgggg
     5701 atgttcaaga tcctgccgcc gcagaagtag ccactcttaa gaagcgccct gcgaaaacat
     5761 cagcgccccc ggaatcactt ccgggggcgc ttcgttctca ggacctcacg actgctgcat
     5821 gaacggatac gggtagtcct tcggcgggac gaagttctcc ttgatggccc gcggcgagac
     5881 ccagcgcacc aggttgagca gcgaccccgc cttgtcgttc gtgcccgagg cccgggcgcc
     5941 accgaagggc tgctggccca cgaccgcacc ggtcggtttg tcgttgatgt agaaattgcc
     6001 ggctgcgtac cgcagtgccg tctccgcctc ccgcaccgcg ccgcggtcct gcgcgaacac
     6061 ggcgccggtg agcgcgtagg gcgaggtgcc gtccaccagg cccagcgtgt ctttccactt
     6121 ggccgccggg tagacgtgca gcgtgagcac gggtccgaag atctcctcgc acatcgtccg
     6181 gtacttggga tcatcggcct ggaggaccgt gggccgcacg aagtagcctt cgcggtcgtc
     6241 gcactcaccg ccgaagagca ccttcgctcc atttcccttc cgcgccccgt cgatgtaccc
     6301 cttgatcttg tcgaacgctt tccggtcgat caccgcgccg acgaagttcg agaagtcggc
     6361 ggggtcgccc atcttgagtg acgagagcat gtcccccatc cgctccttca cgcggggcca
     6421 gatgttgtcg ggcacatacg cgcgtgatgc ggcgctgcac ttctggccct gatactcgaa
     6481 cgcaccgcgg atcagtccgg ccgccagcgc gtccgggtcg gcgctggcgt gggcgagcac
     6541 gaaatccttg ccgcccgtct cccccaccag acgggggtag tccgcgtaat tcggcaggtg
     6601 gtcggacacc cgcttccaga gcatctggaa gacttccgtc gagccggtga agtggatgcc
     6661 gcccaggtgg cggtcggcaa gcagcagatc cgagatctgc ccggagggac ccggcaggaa
     6721 gttgatcacg cccggcggga gaccggcctc gagcaggatc ttcatgatcg cgtagttcga
     6781 cagcgccgcg gtcgccgccg gcttccagac caccgtattg cccatgatgg ccggtgaggt
     6841 cgggagattg ccgccgatcg acgtgaagtt gaagggcgta atggcgtaga cgaagccctc
     6901 gaggggccgg tgatcgaggc ggttccacgt ccccgccggc gacatcggct gttctcccac
     6961 gatccgctca gcgtaatgca cgttgtaccg gaaaaaatcg atcaactcgc aggccgcgtc
     7021 gatctcggcc tggtgcgccg tcttgctctg ccccagcatc gtggtcgcgt tggcaaacgc
     7081 gcggcgcgaa cccgcgagca agtcggccgc cttgagaaac acggccgagc ggtcggccag
     7141 gctccagcgg gaccattcct gccacgcctc gcgtgcggcc ttgatcgccg cctggacctc
     7201 ggcgggcccc gcctgatgca cgttggctag tacctgcttg tgccggtgcg gcgcgacgac
     7261 cggaatgctc tttccggtcc tcacttcctt cccgccgatg atcagcggga tctccatcgt
     7321 ctgcccgctc agctccgcga gcgccccttt cagggtcgcg cgctccgggg tgccgggggc
     7381 gtaggaaagg accggctcgt tgaccggggt cgggattttc gggatcgagt tcataggtcg
     7441 gaatttaacc gcgattggcg gggagcggtt ccccggcccc gaggagtcca cactctgaag
     7501 agtgtgcccg gcacgacact ccgcttaagc ggagtgcatc gccgagccca ggcttcagcc
     7561 tgggaaggac ctgggaaccc gagaatgttt agatagcagg atgaccatcg accccggcac
     7621 caagatcggc cacgtgcacc tgaccgtggc gaacctcgac cgcgcgctgg ccttttaccg
     7681 ggacgtgctc ggtttcgagg tgaccactcg gtatggccgc gaggccgtgt tcctgtcggc
     7741 cggcggctac caccaccatg tggccctgaa cacctgggcc ggtgaagggg cgccggcgcc
     7801 cgcccccggc acgaccggcc tctatcattt cgcgattctc taccccgacc ggaaagcgct
     7861 cgccagggcg ctcaagaacg tcctcgacaa cgggattgtg ctcgagggcg cgtccgacca
     7921 cggcgtgagc gaagcgatct acctccgcga ccccgacggc aacggcgtcg agttgtaccg
     7981 cgaccgcccg caagaagagt ggccgcgtgc cgaggatggg tccctcagaa tgcacacgcg
     8041 gccactgaac ctcgcgcagc tcctcgcaga ggcttgaaca gctacttctt gccggccacc
     8101 ggagcggggt gaaccaccga ggggctgggc ttgggtccga agaagttctg cacttcctgc
     8161 ttcgttcccc ttgggatcat gcccgacaga ttcgccagct cgaccgccga taccgccatc
     8221 accgtggacg cctgctccag gtcccaagga atggccttgt cgtaggtatc cgactgcgaa
     8281 tgatgcgtgt gattgtatcc gcgggtcagc tggtcgaagt tgaacccggg gacgccgtac
     8341 gcgaggaacg acaggtgatc ggtgccgccc ttgttgtggt cgcgcaccga gtcggcgttc
     8401 agggaagcga ccggggccag gatcgacttc cacaggcccg ccagctcggc ccgcccctgc
     8461 agcgcctgac cggtgacggc gccgacgccg ttatccagca cgatcacggc ctgaatggag
     8521 tcggcctcac ccgcatgggc cttggcatac gcctgagagc ccagcagtcc ttcttcttca
     8581 cccgtgaaga gaacgaagcg aatcgtccgc ttcggcttca cgccggactg cgcgatgatg
     8641 cgcgccgctt cgagcgtggc gatggagccg gtgccattgt cggtcgcacc ggtgcccaga
     8701 tcccacgaat ccaggtgccc acccacgatc acaacctgcc cggggtgctc tgagcccttg
     8761 atctccgcga ccgtattcca ttgggacacg ggcttcattg acatcgagct ctggatgttg
     8821 gcctcgatgc gcggcgtgat tccctgcttg atcagccggt cgaacagcgt gtagtcctcg
     8881 tgcgcgatcg cgacctgggg cagtggcagg ggggcgttgg gagaaccgct catggtgagg
     8941 agcgtctgtt ccttggcgcc gtccaccaga atgcccttcg cacccaggtt ccgcagcata
     9001 tacggaaggt cgacgcggaa ctgcgccgct ttggcgcggg ccgccgagtc gagctgcggg
     9061 cgcccgccgt tcccgaaccg cctccgcatg gcctccgaat ccgccgttgt catcggcggg
     9121 ccgtcgttat tccagacgtt ggcgggttcg cgcagcatga gccacgcgcc ccggaccttg
     9181 ctcttgtagg cgtcgagcga gtcctgattg ttcgcgttga tgaggaccac cggaccggcc
     9241 acggtcttcc cgccggtgcc cacggtccac gcccagcttg cgccgatcac gtcatgcttg
     9301 cgcggagccg tcatccggat ccacatggga cccctggtcc acgtgccgcc gaactcccag
     9361 gactcgaggt gcgcgtccag cccgtaatcc ttgaagcgct gcatggtcca gtcgttggcg
     9421 gctcgggctg cgggagaacc
//
LOCUS       DASUBP010000009         4485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_122852, whole genome shotgun sequence.
ACCESSION   DASUBP010000009 DASUBP010000000
VERSION     DASUBP010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122852"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /gene="menC"
                     /locus_tag="VFU75_00455"
     CDS             <1..1141
                     /gene="menC"
                     /locus_tag="VFU75_00455"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HEU5465327.1"
                     /translation="MRILKAELFDLRLPLVEPFIISGGAITERRSLIVALHGEGGHVG
                     YGESPPFEAPFYSEETLGSATLLTRDVLLPRVVGKEFASPEQIDEALREGVRGNPFAI
                     AGVETAAWDLEAHTRGVGLAHLLGERLGELPAKAIPCGVALGIPGDRQLGTLTARVYE
                     AVGRGYRRVKIKVAPGWDAEAVKAARAGMSGTDLPLTVDANGAYRWPRDEKALRALDD
                     AGLLYIEQPLAPDELVGHAELARILRTDLCLDETLRSESHARQVVALGGPKVWNIKVH
                     RMGGLTEFCRIYAVAKAYGAKLWAGTMPESGIGSQAPLAVATLSQFVYPSDLEPSARW
                     FGRNADVIKLSMDAGGMMAVPNQAAGRLLDGGRFRAAASSLGGSS"
     gene            1138..4296
                     /locus_tag="VFU75_00460"
     CDS             1138..4296
                     /locus_tag="VFU75_00460"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465328.1"
                     /translation="MRSLALLLVLLPASLNAQQYPSSLFGGLHWRSIGPFRGGRTVGA
                     TGVPGHPNTFFIGVNDGGVWKTDDAGRTWMPVFDAESTGSIGAVAVAPSDPDVIYVGS
                     GEGLQRPDLSVGDGIYKSTDGGATWTHLGLRDGQQIPAVIVDPRDPNRVFAAVLGHPY
                     GPNPERGVFRSTDGGKTWEKVLYKDENTGAFDVVFDPSNPGTVFATLWAGRQAPWEIG
                     GSFVLSANNGLYKSTDGGTTWRQITAGLPDAGAGLGRIGLGVSPSEPARMYAVVGADS
                     GGALYRSDDGGEHWHAQDTDPRIWGRDGDFNEVKVNPKNADIVYVANVVTWKSTDGGK
                     SFTGWRGAPGGDDYHRLWINPDDPNIILLAGDQGAIITENDGRTWSSWFNQPTAQFYH
                     VSTDNAFPYRVYGGQQESGSAGVWSRGNDGEINFRDWHPVGAEEYGYVAPDPLNPNLV
                     YGGKVSRYDWRTGQVQDVSPEAIRSGKYRWVRTMPLLFSPVDPHTLYLGSNVLFKTRD
                     GGDHWQVISPDLTRTTSDTPSVIRAFAGQDPEHGHHRGVIYTVAPSFKKVALLWAGTD
                     DGLMWVTHDGGLHWGNVTPAALTPWSKVSMMEASHTDTAEAYAAVNRFRLDDLHPHIY
                     RTRDGGKTWAEIVSGLPDDEVVNAVHEDPFKPGLLFAGTERSVYVSFDDGDHWQSLRL
                     DLPATAVRDLTIHESDLVVGTHGRGFWILDDMERLREFTPALASSTHLFRPATAYRVR
                     WNNNTDTPIPPDEPAGENPPDGAIIEYTLAADAKRVEIEIRDSADALVRRFASDDQPP
                     VVDSTVNIPLYWIRPPQTPGASAGSHRFVWDLHGAPVGVLDHGYPISAVVHNTPLEPR
                     GPLVPPGRYLVTLTAGATKVTLPLVVKMDPRVPVTTAALKRQYALATGLAADLKRDSD
                     ALASVRAIRRQLAALRTNPAAGSLKPAIDSADTQLAGFESDRKGRTHRDASLTGLNAD
                     LGAVYGIVEGADAAPTSQVEREVVDLKLDLELTLNLTKAFTQAVVPPLNQRLKKAGLP
                     ALSLDAREPVQGHVGNGEADEP"
BASE COUNT          743 a         1473 c         1531 g          738 t
ORIGIN      
        1 catgcgcatc ctcaaggctg agctcttcga cctccgcctg cccctggtcg agcccttcat
       61 catctccggg ggcgcgatca cggaacggcg ttctctcatc gtcgcgctgc acggcgaggg
      121 ggggcacgtc ggctatggcg aatcgccgcc cttcgaggcc ccgttctatt ctgaagagac
      181 actcggttcc gccacgctgc tcacgcgcga cgtgcttctc ccccgtgtgg tggggaagga
      241 gttcgcgtcc cctgaacaaa tcgacgaggc tctgcgggag ggtgtgcggg gcaatccctt
      301 cgcgatcgcg ggtgtcgaaa ccgcggcgtg ggatctcgag gctcacacgc ggggggtggg
      361 cctcgcgcat ctgctcggcg agcggctcgg ggaacttccc gccaaggcca tcccgtgcgg
      421 cgtggcgctc gggattccgg gcgatcgcca gctcgggacg ctcaccgctc gcgtctacga
      481 ggcggtgggg cgcggatatc gcagggtcaa gatcaaggtc gcgccgggat gggacgccga
      541 agcggtgaaa gccgcgcggg ccgggatgtc cgggaccgat ctccccctca cggtggatgc
      601 gaacggggcc tatcgttggc cgcgggatga gaaggccctg cgcgcgctcg acgacgccgg
      661 tctcctctat atcgaacagc ccctggcccc ggatgagctc gtgggacacg cggagctcgc
      721 acggattctc aggaccgatc tctgtctcga tgagactctc cgcagtgaga gccacgcgag
      781 gcaggtcgtc gctcttggcg gcccgaaggt gtggaacatc aaggtgcacc gcatgggtgg
      841 gctcacggag ttctgccgca tctacgcggt ggcgaaagca tatggcgcga agctgtgggc
      901 tggaacgatg cccgagtcgg ggatcggttc gcaggcaccc ctcgccgtcg cgacgctctc
      961 gcagttcgtg tatccctccg acctcgagcc ctcggctcgc tggttcgggc ggaacgccga
     1021 cgtgatcaag ctcagcatgg acgcgggcgg catgatggcc gtccccaacc aggcggccgg
     1081 aagactgctc gatggcgggc gcttccgggc cgcggccagc tccctcggag gctcgtcgtg
     1141 agatcgcttg cgctgctgct cgttctcctt cccgcgtcac tcaacgccca gcaatacccg
     1201 tcgtccctct tcgggggact gcactggcgc tcgatcggtc ccttccgcgg gggccggacg
     1261 gtcggggcga ccggggtccc gggccacccc aacacgttct tcatcggggt gaacgacggc
     1321 ggagtctgga agaccgatga cgcggggcgt acgtggatgc cggtattcga cgccgagtcg
     1381 acggggtcga tcggggcggt ggcggtggcg ccctcggatc ccgacgtcat ctacgtcggg
     1441 agcggggaag ggctgcagcg gcccgacctg tcggtgggcg acggcatcta caagtcgacg
     1501 gatggcggcg cgacctggac ccatcttggc ctgcgcgacg gacagcagat ccccgcggtg
     1561 atcgtggatc cacgcgatcc caaccgcgtg ttcgcggccg tattgggtca tccctatgga
     1621 ccgaacccgg agcggggcgt gttccgctcc acggatggcg ggaagacctg ggagaaggtg
     1681 ctgtacaagg acgagaacac gggtgccttc gacgtggtgt tcgatccgtc gaatcccggg
     1741 accgtcttcg cgacgctgtg ggccggacgc caagcgccct gggaaatcgg gggttcgttc
     1801 gtcctttccg ccaacaacgg cctgtacaag tcgaccgatg gcggcaccac ctggcgccag
     1861 atcaccgcgg ggctcccgga cgccggcgcg gggttgggcc gcatcggact cggcgtgtcg
     1921 ccgtcggagc cggcccggat gtacgcggtc gtcggtgcgg actccggagg cgcactgtac
     1981 cgctccgatg acggcggcga gcattggcac gcccaggaca cggatccccg catctgggga
     2041 cgtgacgggg atttcaacga agtgaaggtg aatccgaaga acgccgacat cgtgtacgtc
     2101 gcgaacgtcg tcacgtggaa atccaccgac ggcgggaagt cgttcaccgg atggcggggg
     2161 gctcccggcg gcgacgacta ccaccggttg tggatcaacc cggacgaccc gaacatcatc
     2221 ctgctcgccg gcgatcaggg tgccatcatc accgagaacg acgggcgtac gtggagctcg
     2281 tggttcaacc agccgaccgc gcagttctat cacgtctcga ccgacaacgc cttcccctat
     2341 cgcgtctatg gcggacagca ggaaagcggc tcggcgggag tgtggagccg cggcaatgac
     2401 ggcgagatca atttccggga ttggcatccg gtaggtgccg aggagtacgg gtatgtggcg
     2461 cctgatccgc tgaatccgaa cctcgtctac ggcggcaagg tcagccgcta cgactggcgc
     2521 accggccagg tacaggacgt ctcgccggag gcgatccgct ccggcaaata ccgttgggta
     2581 cggacgatgc ctctgctgtt ctcgccggtc gatccccata cgctctatct gggatccaac
     2641 gtgctgttca agacccggga tggcggagac cactggcagg tcatcagtcc cgatctcacg
     2701 cgcacgacct cggacacgcc gtcggtcatt cgtgcgttcg cgggccagga cccggagcat
     2761 ggccatcacc ggggcgtgat ctacacggtg gcgccgtcgt tcaagaaggt cgcgctcctc
     2821 tgggcgggga cggacgacgg gctgatgtgg gtgacgcatg atggcggctt gcactggggg
     2881 aacgtcacgc cggcagcact cacgccctgg agcaaagtgt cgatgatgga ggcgtcacac
     2941 acggacaccg cggaggcgta cgcggccgtg aaccggttcc ggctggacga tctgcacccg
     3001 cacatctatc ggacacggga cggcgggaag acgtgggccg agatcgtgtc cggcctcccc
     3061 gacgacgaag tcgtgaacgc cgttcacgaa gatccgttca agccgggatt gctcttcgcg
     3121 ggaaccgagc gctcggtcta tgtctccttt gatgacggcg atcattggca gtcactgcgg
     3181 ctcgatttgc ccgcgacggc ggtgcgcgac ctgaccattc acgagagcga tctggtggtg
     3241 ggaacgcacg gccgggggtt ctggattctc gacgacatgg agcggctgcg agagttcaca
     3301 ccggcgctcg cgtcctccac gcacctcttc cgtcccgcga cggcgtaccg ggttcggtgg
     3361 aacaacaaca ccgacacgcc cattccgccc gacgaacccg cgggagagaa tcctcccgac
     3421 ggcgcgatca tcgagtacac gttggcggcg gacgcgaagc gcgtggagat cgagatccgc
     3481 gattcggctg acgccctggt ccggcgattt gccagtgacg accagccgcc cgtcgtcgat
     3541 tcgacggtga acatccccct gtactggatc aggcctccac agacgccggg tgcgtcggcg
     3601 ggctcacacc ggttcgtctg ggacctccac ggtgctccag taggcgtact ggaccacgga
     3661 tatccgattt cggcggtggt gcacaacacg cctctggagc cccgcggccc gctcgtcccg
     3721 ccggggcggt acctggtgac gctcaccgcc ggcgccacca aggtgacact gccgctcgtg
     3781 gtgaagatgg acccgcgcgt tccggtcacc acggcggcgc tcaaacgcca gtatgcactg
     3841 gccacgggcc tggcggccga cttgaaacgg gactctgacg ctctcgcgtc ggtccgcgcg
     3901 atccgtcgcc agctcgcggc ccttcgcacc aatcccgcgg ctggctccct gaaaccagcc
     3961 atcgactcgg cggatacgca gctggcaggg ttcgagtcgg accggaaggg aaggacccac
     4021 cgggatgcct ccctcaccgg actgaacgcg gatctggggg cggtctacgg gatcgtcgag
     4081 ggagccgacg cggcccccac cagccaggtg gagcgggagg tcgtcgacct caagctggat
     4141 ctggagctga ccttgaacct gaccaaggcc ttcacgcagg ccgtcgtgcc cccactgaat
     4201 cagcggctca agaaggcggg gcttccggcc ctttcgcttg acgctcgcga gcccgtccag
     4261 gggcatgtcg gcaacggtga agcggatgag ccatgaccac ctagccgtct agccgtctag
     4321 ccgtctagct tgtggtccat gacgaccccc tccgcagcct attgggcgac gaacctgtat
     4381 cccagtcccc gccagcgccc gaaaaccgac tctcctattc gggatttgcc ccagcgcgcc
     4441 gcgggccggt tcaaacgcgc acgcgagggc atccgggcgc tcaag
//
LOCUS       DASUBP010000010         2801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_177414, whole genome shotgun sequence.
ACCESSION   DASUBP010000010 DASUBP010000000
VERSION     DASUBP010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2801
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177414"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /gene="rpsL"
                     /locus_tag="VFU75_00465"
     CDS             complement(<1..362)
                     /gene="rpsL"
                     /locus_tag="VFU75_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU5465329.1"
                     /translation="MPTINQLVRHGRKTVGGKEKAPALRGNPQKRGVCTRVYTTTPKK
                     PNSALRKVARVRLTNGFEVTAYIPGEGHNLQEHSIVMIRGGRVKDLPGVRYHIIRGTL
                     DSAGVTDRKQSRSKYGAKR"
     gene            503..862
                     /locus_tag="VFU75_00470"
     CDS             503..862
                     /locus_tag="VFU75_00470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465330.1"
                     /translation="MRLVPVLLLVAATPVAAQQLPPSHPRYTSTFARTQTPPLIQPGD
                     YAWEGAKVGAVIFGLPGIGVGYALCGDADNGHPTSFAYCAPRALIGGLIGATLGAVVG
                     GYIGSTIHRDPETAPPQ"
     gene            complement(918..>2801)
                     /locus_tag="VFU75_00475"
     CDS             complement(918..>2801)
                     /locus_tag="VFU75_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU5465331.1"
                     /translation="GARKGLADTALKTADAGYLTRRLVDVAQDVTITEEDCGTILGLE
                     MTALKEGEDIIEPLRERIVGSVPADDVFDPHELDEDGDQKLLVAAGGLITEEISEAIE
                     DAGIEQVKIRSVLTCEARRGVCRMCYGRNLATMDMVDLGEAVGILAAQSIGEPGTQLT
                     LRTFHIGGTAARIAEQTVRKTKVEGVIEYGDRLAFVVTPENLKVVTSYEGELVLKAAA
                     HGDQKLSVHSRFHVPLGATLVAEDGAKVAKDGVLFTWDPYTNPIMTDVAGIVRFVDIV
                     EDETVREELDEITGRRQRVIIEDREKKLHPHIEVVQKGKGDKEKRLRDFVIPEGAQLT
                     IEDGAEVYAGQTLAKVSREAYKTRDITGGLPRVAELFEARRPKDPATISEVDGTVRFG
                     GIKRGKREIFIHAEEGEPQLYEVPAGKHLRVHEGDRVRAGDRLTEGPVNPHDILRIKG
                     PRAVQEYLLNEVQEVYRLQGVTINDKHIGVIVRQMLQKVKTLEPGDTEFLEGENVDKL
                     VFKDQNERVTKKGGTPATAEPLLLGITKASLTTQSFISAASFQETTRVLTDAAIRGAK
                     DDLLGLKENIIIGHLIPAGSGIYRYQEVEIEPPEGFEPPTPPQELAPGTVPSPFPEEA
                     EVE"
BASE COUNT          402 a         1000 c          850 g          549 t
ORIGIN      
        1 cgcttggcac cgtacttgga gcgcgactgc ttccgatcgg tcaccccggc cgagtccagc
       61 gtgccgcgga tgatgtgata ccgcacgccc ggcagatcct tcacgcggcc gccgcggatc
      121 atcacgatcg agtgctcttg aagattgtgc ccttcgcccg ggatgtacgc cgtcacttcg
      181 aacccattgg tcagacggac acgcgccact tttcgaaggg ccgagttcgg cttcttggga
      241 gtggtggtgt agacgcgggt gcagactccg cgtttctgtg ggttcccgcg cagcgcgggc
      301 gccttttcct tccctcccac ggtcttccga ccatggcgga ccagctgatt gatcgtcggc
      361 atacgttccg ggtcaggctc caaaaaagtc gggaaataga cttcaaagct aaggggggac
      421 ttggacttgg gtcaaccccg accccttgga aggcccttcg ggtagggcgg gcattttgtc
      481 ctgtgaaggg taggattatc cgatgcgcct cgtccctgtc ctcctcctcg ttgcggcgac
      541 gccggtcgct gcccaacagc tgcccccaag tcatccccgc tacacgtcga cgtttgcccg
      601 tacccagacc cctcccctga ttcagccggg cgactacgcc tgggaggggg ccaaggtcgg
      661 ggcggtgatt ttcggcctcc ctggaatcgg cgtcgggtac gcactctgcg gagacgccga
      721 caacggacac cccaccagct tcgcctactg cgctccgcga gcgctgatcg ggggcctgat
      781 cggtgccacc ctgggtgccg tcgtgggtgg gtacatcggg agcaccattc accgggatcc
      841 tgagaccgct cccccccaat agcaaaaaaa cggggcgtcc tttcggacgc cccgtttcgc
      901 tttccacccc aacccggtta ctcgacttcg gcttcctccg ggaagggact cgggaccgtc
      961 cccggcgcca gctcctgcgg cggcgtgggc ggctcgaagc cctcgggcgg ctcgatctcc
     1021 acttcctggt accggtagat gccggacccg gccgggatca agtgcccgat gatgatgttc
     1081 tccttcagcc cgagcaggtc gtccttcgcg ccgcgaatgg ccgcgtcggt cagcacccgc
     1141 gtggtctcct ggaacgacgc cgccgagatg aacgactgcg tcgtcagcga agccttcgtg
     1201 atgccgagga gcagcggctc ggccgtcgcc ggcgtgccgc ccttcttcgt cacccgctcg
     1261 ttctgatcct tgaagaccag cttgtccacg ttctcccctt ccaggaattc cgtgtcgccg
     1321 ggctccaacg tcttcacctt ctgcagcatc tggcgcacga tcacgccgat gtgcttgtcg
     1381 ttgatcgtca cgccctggag ccggtagacc tcctgcactt cgttcaggag gtattcctgc
     1441 accgcccgcg ggcccttgat ccgcagaatg tcgtgcggat tcaccggacc ttcggtcaac
     1501 cggtcaccgg cgcgcacgcg gtcaccctcg tgcacccgca agtgcttccc ggcgggcacc
     1561 tcgtacagct gcggctcgcc ctcctcggcg tgaatgaaga tctcgcgctt gccgcgcttg
     1621 atcccgccga agcggaccgt gccgtcgact tcggagatcg tggccggatc cttcgggcgc
     1681 cgcgcttcga acaactcggc cacccgcggc agaccgccgg tgatgtcgcg cgtcttgtag
     1741 gcttcgcggg acaccttggc cagcgtctga ccggcgtaca cctcggctcc gtcctcgatg
     1801 gtcagctgcg cgccttccgg aatgacgaag tcgcggagcc gcttctcctt gtcgcccttc
     1861 cccttctgca ccacctcgat gtgcgggtgg agcttcttct cgcggtcttc gatgatgacc
     1921 cgctggcgcc ggccggtgat ttcgtccagc tcctcgcgga ccgtctcgtc ttccacgatg
     1981 tccacgaacc gcacgatgcc cgccacgtcc gtcatgatcg ggttcgtgta cggatcccac
     2041 gtgaacagca cgccgtcctt cgcgaccttc gcgccatcct cggccaccag cgtggcgccg
     2101 aggggcacgt ggaaccgcga gtgcacggac agcttctgat cgccgtgagc ggcggccttg
     2161 aggaccagct cgccctcgta gctcgtcacc accttcaggt tctccggcgt cacgacgaac
     2221 gccaggcggt cgccgtactc gatcacgcct tcgaccttgg tcttccgcac cgtctgctcc
     2281 gcgatacgcg ccgcggtgcc gccgatgtgg aaggtccgga gggtcagctg cgtacccggc
     2341 tcgccgatcg actgcgccgc caggatgccg accgcctcgc ccaggtccac catgtccatg
     2401 gtggccaggt tccggccgta gcacatccgg cagacgccgc gacgggcttc gcaggtgagc
     2461 acggagcgga ttttgacctg ctcgatcccg gcgtcttcga tcgcctctga gatctcctcc
     2521 gtgatcagcc cacccgcggc caccaggagc ttctggtcgc cgtcctcgtc gagctcatgc
     2581 ggatcgaaga cgtcgtcggc cggcacggag cccacgatgc gctcgcggag cggctcgatg
     2641 atgtcttcgc cctctttcag cgcggtcatc tcgaggccga ggatggtgcc gcaatcttcc
     2701 tcggtgatcg tgacgtcctg cgccacgtcc accagcctcc gggtcaggta gccggcgtcg
     2761 gccgtcttga gcgccgtatc cgccagaccc ttccgggcgc c
//
LOCUS       DASUBP010000011         4359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_449954, whole genome shotgun sequence.
ACCESSION   DASUBP010000011 DASUBP010000000
VERSION     DASUBP010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449954"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFU75_00480"
     CDS             <1..351
                     /locus_tag="VFU75_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465332.1"
                     /translation="SGVNQINQAMGQVDQVTQRGAAAAEELASTATELAAQAESLQRL
                     VAFFRVDQETTHRAEAIALATLAGEQSGKSNGHGSSHGNGHGNGHGAVKANGHESRIP
                     ATTAPAAEPHFTRF"
     gene            371..859
                     /locus_tag="VFU75_00485"
     CDS             371..859
                     /locus_tag="VFU75_00485"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEU5465333.1"
                     /translation="MTTTKTDPLQTQYLTFVVGEEEYGVRILRAKEIIEYGVVTTIPN
                     APPFIRGVINLRGSVVPVVDLAVKFGGAGSVPTKRSCVVVVEVERDGQQTVMGIVADR
                     VTQVAELPDDAIEPAPSFGTGVRTDWLLGLGRTDRRFILLLNTDRVLTDLDIGPLEVA
                     AA"
     gene            856..1737
                     /locus_tag="VFU75_00490"
     CDS             856..1737
                     /locus_tag="VFU75_00490"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEU5465334.1"
                     /translation="MTFVTDFAVPGLTDAEFVQFQRLIHQRAGIVLSDAKRALLVGRL
                     ARRMRELGLSSFSAYYAAVQAGGQAEMTRLLDAICTNETHFFREGAQFDLLRDRVCSE
                     WLGDEEAGRRARTVKVWSAACSTGEEPHSIAMVLEDRLGRLGWGIDILATDLSTKVLD
                     QASAGVWRLDKATPIPEHYLKSYMLKGVGPEAGKVKAGAAIRSRIRFSQVNLRETDSG
                     WPVRERFDAIFVRNVLIYFDAPTRHDVLRRLVGHLNPGGYLFLGHAEGMAGAALPLKS
                     VMPAVYRLANATAGRAA"
     gene            1734..2816
                     /locus_tag="VFU75_00495"
     CDS             1734..2816
                     /locus_tag="VFU75_00495"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HEU5465335.1"
                     /translation="MSKSLHVLVVDDSAVVREAMAAVLADGEGITVATAPNPIVARRR
                     MALKRPDVIVLDLDMPEEDGLTFLRSQMAQDPIPVVICSSHSVQGSRAALQALHDGAV
                     EVVAKPRIGVREFVQESKIILLDAIRGAAAARLRRRGPVVAAPAPRVAAPPPVSGGSS
                     RRVIAMAASTGGPQALHEVLAALPAETPGILVVQHMPAGFTTAFAKHLDERCAMTVRE
                     AEAGDEVQDGLVLVAPGGRHLSLKRSRTGFAAEVTDGDLVSRHRPSADVLFRSVAEQV
                     GGEALGVVLTGMGDDGAEGLAAMKAAGAGTVAQDEATCVVYGMPKEAVARGAIDVVLP
                     LSQVADCISGWTRKRRRERITTGEEQ"
     gene            2813..3175
                     /locus_tag="VFU75_00500"
     CDS             2813..3175
                     /locus_tag="VFU75_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014476812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5465336.1"
                     /translation="MSGTVLLCDDAIFTRMMLTGFVEKAGYEVVGEASNGKEAVSQYE
                     RLKPDVVLMDVVMPDMSGLDAVREIRKKDGSAKIVMCSAMGQEKIMHEAIEAGASGFI
                     VKPFTAARVLEALGDAEQ"
     gene            3172..3789
                     /locus_tag="VFU75_00505"
     CDS             3172..3789
                     /locus_tag="VFU75_00505"
                     /inference="COORDINATES: protein motif:HMM:NF025070.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HEU5465337.1"
                     /translation="MSAGAQLDPAGIEIDALREVANIASGHAATALGQLTDRKVWISI
                     PEFTLTRAEEVPQVLGYADRKVVVIAMQVLGDVTGSLVFLMPEERARRLAGLLLGQTE
                     PAQGEFEGLIKSSLMETANIICGAYAGALASLTNRMIMLSVPTFGVEPPDEILAGQDI
                     TKGQLGLCIESSLSLSGETVPFGGHILLLPHPAALRLILDALNVF"
     gene            complement(3931..>4359)
                     /locus_tag="VFU75_00510"
     CDS             complement(3931..>4359)
                     /locus_tag="VFU75_00510"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5465338.1"
                     /translation="GVNFASGRLTARYGARPPLVWGLTIGGLGLLGLGQLTATSALGV
                     AVLPMLAVGVGSALTMPAMTATAIEHTPKERAGIGAAVLNAARQVGGVVGIALLGAFV
                     AGTHFMSGFPWGMRLAGALFLGGAVLSWVSVERGPSLRAL"
BASE COUNT          734 a         1363 c         1609 g          653 t
ORIGIN      
        1 tcgggcgtca atcagatcaa tcaggccatg ggccaggtgg accaggtgac ccagcgcggc
       61 gccgcggcgg ctgaagagct cgcctcgacc gccacggagc tcgcggcgca ggccgagtcg
      121 ctccagcgcc tggtggcgtt cttccgggtc gatcaggaaa ccacgcaccg cgccgaggcc
      181 atcgccctgg cgacgctcgc cggggagcag tccggcaaga gcaacggtca cggcagtagc
      241 cacggcaacg ggcacggcaa cgggcacgga gcggtgaagg cgaacgggca cgagtcccgg
      301 attccggcta cgaccgctcc cgcggccgag ccgcacttca cgcggttcta accgagagga
      361 tccaagggct atgacgacca cgaagacgga tcctctccag acgcaatacc tcacgttcgt
      421 ggtgggggaa gaggaatacg gcgtccgcat cctgcgggcg aaagagatca tcgagtacgg
      481 cgtcgtgacc acgattccca acgcgccgcc gttcatccgg ggggtcatca acctccgggg
      541 cagcgtggtg ccggtggtgg atctggcggt gaagttcggg ggcgcaggat cggtccccac
      601 caagcggagc tgtgtggtgg tagtggaagt ggagcgggac ggccagcaga cggtgatggg
      661 catcgtggcc gaccgcgtga ctcaggtggc ggagctgccg gacgatgcca tcgagccggc
      721 gccgtccttc gggaccgggg tccggaccga ctggctgctg ggcctggggc gcaccgaccg
      781 ccggttcatc ttgctgctca acacggaccg ggtgctgacc gacctggaca tcgggccgct
      841 ggaagtcgcc gcggcatgac gttcgtcacg gatttcgcgg taccgggact gacggacgcg
      901 gagttcgtcc agttccagcg gctcattcac cagcgagccg ggatcgtctt gtcggatgcg
      961 aaacgcgccc tgctggtggg gcgcctcgcg cggcggatgc gggagctggg actgtcgtcc
     1021 ttcagcgcct actacgccgc ggtgcaggcc ggcggccagg cggagatgac gcgcctcctg
     1081 gacgccatct gcacgaacga aacgcacttt ttccgggaag gagcacagtt cgacctgctc
     1141 cgggaccggg tgtgcagcga gtggctgggc gatgaagagg cgggccggcg ggctcggacg
     1201 gtcaaagtct ggagtgccgc ctgctcgacg ggtgaggagc ctcactccat cgcgatggtg
     1261 ctggaggacc ggctcggccg gctggggtgg gggatcgaca tcctcgcgac cgacctctcg
     1321 accaaggtgc tcgatcaggc ctcggccggg gtgtggcggc tcgacaaggc gacgccaatc
     1381 ccggagcact acctgaaatc ctacatgctg aaaggcgtgg gaccggaagc gggcaaggtc
     1441 aaggcgggag cggcgatccg gagccgcatc aggttctcgc aggtgaacct gcgggagacg
     1501 gattcggggt ggccggtgcg cgagcggttc gacgcgattt tcgtccgcaa cgtgctgatc
     1561 tatttcgatg cccccacacg ccacgacgtg ctgcggcgcc tggtggggca tctgaatcca
     1621 ggcggctacc tgttcctcgg ccacgccgag gggatggcgg gcgcggcgtt gccgctcaag
     1681 tcggtgatgc ccgcggtgta ccggctggcc aacgcgacgg cgggacgggc ggcgtgagca
     1741 agtccctcca cgtgctggtc gtggacgatt ccgccgtggt gcgggaagcg atggcagccg
     1801 tgctggcgga cggcgagggc atcacggtcg cgacggcgcc gaacccgatc gtggcccggc
     1861 gccggatggc gctcaagcgg cccgatgtga tcgtgctgga tctcgacatg ccggaggagg
     1921 acgggctgac cttcctccgc tcgcagatgg cccaggatcc gattccggtc gtcatctgct
     1981 cgagccacag cgtgcaggga tcgcgggccg cgttgcaggc gcttcacgat ggcgcggtgg
     2041 aagtcgtcgc gaagccgaga atcggtgttc gggagttcgt ccaggagtcg aagatcatac
     2101 tcctggatgc catccgcggt gccgccgccg cgcggttgcg gaggcgggga ccagtggtgg
     2161 ccgctccggc gccgcgcgtg gccgcaccgc caccggtgtc gggcggctcg agccgacggg
     2221 tgatcgcgat ggcggcctcg acgggtggac cccaggcgct gcatgaagtc ctcgcggcgc
     2281 tgccggcgga gacgcccgga atcctggtcg tgcagcacat gccggcggga ttcacgaccg
     2341 ccttcgcgaa gcacctcgat gagcggtgcg cgatgacggt acgcgaagcg gaagccgggg
     2401 acgaagtgca ggacggcctg gtgctggtcg cgccgggagg ccggcacctg agcctcaagc
     2461 ggtcacggac cgggttcgcg gcggaagtga cggacggcga cctcgtgtcc cgtcaccggc
     2521 cgagcgccga cgtgctgttc cgctcggtgg cggagcaggt gggtggggag gccctcggcg
     2581 tggtcctcac cgggatggga gacgacgggg ccgaagggct ggccgcgatg aaggcggccg
     2641 gggcggggac cgtggcgcag gacgaagcca cgtgcgtcgt gtacggaatg ccgaaagaag
     2701 cagtcgcgcg gggcgcgatt gatgtggtgt tgccgctttc gcaggtcgcc gactgcatca
     2761 gcggatggac caggaagcgg cgtcgtgaac ggataactac gggagaagaa caatgagcgg
     2821 gacggtgttg ctgtgcgatg acgcgatctt cacgcggatg atgttgaccg ggttcgtgga
     2881 gaaggccggc tacgaagtag tcggggaagc gtcgaacggg aaggaagctg tgtcacagta
     2941 cgagcggctg aagcccgacg tggtattgat ggacgtggtg atgccggaca tgagcgggct
     3001 cgacgcggtg cgggagatcc gcaagaagga cgggtcggcc aagatcgtca tgtgcagcgc
     3061 catggggcag gagaagatca tgcatgaagc gatcgaggcc ggcgcgtccg gcttcatcgt
     3121 aaagcccttc accgcggccc gcgtcctcga ggcgctgggc gacgccgagc aatgagcgcg
     3181 ggcgcgcagc tcgacccggc cggcatcgaa atcgatgcgc tccgggaggt tgccaacatc
     3241 gcgagcgggc acgccgccac ggcgctgggc cagctcacgg accggaaggt gtggatctcg
     3301 atccccgaat tcaccctgac gcgagccgaa gaggttcctc aggtcctcgg gtacgcggac
     3361 cggaaggtcg tcgtcatcgc gatgcaggtg ctgggcgacg tcaccgggtc gctggtcttc
     3421 ctgatgcccg aagagcgggc ccgccggctg gcgggattgc tcctggggca gaccgagccc
     3481 gcccagggtg agttcgaggg attgatcaag tcgtcgttga tggagacggc gaacatcatc
     3541 tgcggggcgt atgccggcgc gctcgcgtcc ctcaccaacc ggatgatcat gctctcggtg
     3601 ccgaccttcg gcgtcgagcc acccgatgag atcctggcgg gtcaggacat tacgaagggt
     3661 cagttgggct tgtgcatcga gtcgagcctg agcctgtcgg gagagacggt gccgttcgga
     3721 ggacacattc tcctgctgcc ccatcccgcc gcgctgcggt tgatcctcga cgcgctgaac
     3781 gtgttttaag aagcaggtcg cttcgcgcct tcggcaacac ctacggagca cgacaatcag
     3841 gtccttcgcg ccttcggcgc tcaggatgac atgctcgctc ctcctctttc aagaaaagga
     3901 gagtagccct gtcatcctga gcgacgcgcg ctacagcgcg cggagcgaag gaccccgctc
     3961 caccgacacc cagctcagga ccgccccgcc cagaaagagg gcgccggcga gccgcatccc
     4021 ccacgggaat ccgctcatga agtgggtgcc ggcgacgaac gcgcccagga gcgcgatgcc
     4081 gacgaccccc ccgacctgac gggccgcgtt gagtacggcg gctccgatcc ccgcgcgctc
     4141 cttgggcgtg tgctcgatgg ccgtcgcggt catcgccggc atggtgagcg ctgaaccgac
     4201 gccgacggcg agcatcggca gcaccgccac gccgagggcc gacgtcgccg tgagctgtcc
     4261 cagccccagc agtcccaggc ctccgatcgt caaaccccac accagaggcg gtctcgcgcc
     4321 gtagcgcgcg gtcagccgcc ccgacgcgaa gttcacgcc
//
LOCUS       DASUBP010000012         2469 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1294798, whole genome shotgun sequence.
ACCESSION   DASUBP010000012 DASUBP010000000
VERSION     DASUBP010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2469)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2469)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2469
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294798"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..1206)
                     /locus_tag="VFU75_00515"
     CDS             complement(4..1206)
                     /locus_tag="VFU75_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEU5465339.1"
                     /translation="MPVFEYTARTLKGELVRDKIDLPTKDDVIAHIRKNKMILVQVVQ
                     GAKSIGFKFGGGVKTRDVVIFTRQFATMINSGLPLVQALDILAQQTENKALAEITRQV
                     VYDVESGHTLADALRKHPKGFTDLYVNMVAAGEAGGILDTILLRLAQFLEKNDAIVRK
                     VKGAMIYPAVIFSVAAIAVAVLLIFVIPTFQNMFASVNLQLPLPTRIVIGMSSILIHY
                     WWLIIGVIGGSIYAINRYYKTAPGKLQIDTMMLRAPVLGDLLRKSAVSRFTRTLGTLI
                     SSGVSILDGLEITARTAGNMVIHNAVMESRASIAGGETISAPLQKSKVFPPMVISMIA
                     VGEQTGGLDEMLSKIADFYDEEVDAAVSALLSLMEPIMIVVLGVIVGGMVVAMYLPIF
                     DMVNAVQG"
     gene            complement(1227..2360)
                     /locus_tag="VFU75_00520"
     CDS             complement(1227..2360)
                     /locus_tag="VFU75_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEU5465340.1"
                     /translation="MNLRALLEEMIEKDASDLHIVAGECPKIRVDGDIVSASGSEVLT
                     PKDTLSLAYSVLTENQKKRFETENELDFSFGIQNLARFRGNCFKQRGCVSLVIRQIPF
                     NVKTFDDLGLPPIIAKLTEKPRGLVLVTGPTGSGKSTTLAAMIDKINKEMKGHIITVE
                     DPIEFIHRHQSCIVNQREVGTDTNSFQAALKYALRQDPDVVLVGEMRDLDTIQAALTI
                     AETGHLAFATLHTNSAAETINRVIDVFPSHQQSQIRAQLAFVLEGVLTQTLLQRAKGR
                     GRIMAAEIMVCTPAIRALIRDDKVHQIESSMQAGKKYGMQTLNDALYQLYMAREVAKE
                     ECLRVSSNPNEFLRQIGEPPMDDREPGSPAGAQAGGAKVAARR"
BASE COUNT          419 a          748 c          801 g          501 t
ORIGIN      
        1 gcgtcacccc tggacggcgt tcaccatgtc gaagatcggc aggtacatgg cgaccaccat
       61 gccgcccacg atgacgccga ggacgacgat catgatgggt tccatgaggg agaggagggc
      121 ggagacggct gcgtcgacct cttcgtcata gaagtccgcg atcttcgaaa gcatttcgtc
      181 gagaccgccg gtctgctcgc cgaccgcgat catcgagatc accatgggcg ggaagacctt
      241 ggacttctgg agcggcgccg agatggtttc gccgcccgcg atcgaggcgc gcgattccat
      301 cacggcgttg tggatgacca tgttgcccgc ggtgcgcgcc gtgatctcga ggccgtcgag
      361 aatcgacacg cccgatgaga tcagggtgcc gagggtgcgg gtgaagcgtg agacggccga
      421 cttacggagc aggtcaccca ggaccggcgc gcggagcatc atggtatcga tctgcagctt
      481 cccgggcgcg gtcttgtagt atcggttgat cgcgtagatc gagccgccga tcacgccgat
      541 gatgagccac cagtagtgga tcaggataga gctcatcccg atgacgatcc gcgtggggag
      601 cggcagctgc aggttcacgg acgcgaacat gttctggaag gtcggaatca cgaaaatgag
      661 cagcaccgcc accgcgatgg ccgccaccga gaagatcacg gcgggataga tcatcgcgcc
      721 tttgaccttc cggacgatgg cgtcgttctt ttcgaggaac tgtgccagcc ggagcagaat
      781 ggtgtcgaga ataccgcccg cctcgccggc ggccaccatg ttgacgtaga ggtcggtgaa
      841 gcccttcggg tgcttgcgga gcgcgtcggc cagcgtgtgg cccgactcca catcgtacac
      901 cacctggcgg gtgatttccg ccagggcctt gttttccgtc tgctgggcca ggatgtcgag
      961 cgcctggacc agggggaggc ccgagttgat catggtggcg aactgacggg tgaagatcac
     1021 gacgtcccgg gtcttgaccc cgccgccgaa cttgaagccg atcgacttcg caccctggac
     1081 gacctgcacc aggatcattt tgttcttgcg tatatgcgcg atgacgtcgt ctttggtcgg
     1141 gaggtcgatc ttgtcccgga ccagctcgcc cttcagcgtt cgcgcggtat actcgaaaac
     1201 cggcatgtcc agcctcctct agtcgtctac cgccgtgccg cgaccttcgc cccacccgcc
     1261 tgggcgccag ccggtgagcc cggctcccgg tcgtccatcg ggggttcgcc gatctggcgc
     1321 aggaactcgt tcggattgct cgagacccgg aggcactcct ctttcgccac ttcccgggcc
     1381 atgtagagct gatagagcgc gtcgttcaac gtctgcatgc cgtacttctt ccccgcctgc
     1441 atggacgatt cgatctggtg gaccttgtcg tcacggatca gggcccggat ggcgggagtg
     1501 cacaccatga tctccgcggc catgatgcgg ccgcgtcctt tggcccgctg caacagcgtc
     1561 tgcgtcagca cgccttcgag gacgaacgcc agctgagcgc ggatctgcga ctgctggtga
     1621 gacgggaaga cgtcgatgac acggttgatg gtctcggccg ccgagttggt gtgcagggtg
     1681 gcgaaggcca ggtgtccggt ctcggcgatg gtcagggccg cttgaatcgt gtccagatcg
     1741 cgcatttcgc cgacgagcac gacgtccggg tcctgccgca acgcgtactt cagcgcggcc
     1801 tggaaactgt tggtatcggt gccgacctca cgctggttca cgatgcacga ctggtgacgg
     1861 tggatgaact cgatcgggtc ctccaccgtg atgatgtggc ccttcatttc cttgttgatc
     1921 ttgtcgatca tcgccgcgag cgtggtgctc ttgcctgagc ccgtcggccc ggtgacgagc
     1981 accagaccgc gcggcttttc cgtgagcttg gcgatgatgg gcgggagccc gagatcgtcg
     2041 aaggtcttca cgttgaacgg gatctgccgg atgacgagcg agacgcaccc gcgctgcttg
     2101 aagcagtttc cgcggaagcg cgccaggttc tggatgccga acgaaaagtc cagctcgttt
     2161 tcggtttcga accgtttctt ctggttttcc gtcaggaccg agtacgcgag ggacagcgtg
     2221 tccttgggcg tcaggacttc gctgccggag gcgctgacga tgtcgccgtc gacccggatc
     2281 ttgggacact ccccggccac gatgtggagg tcggaggcgt ccttctcgat catctcttcg
     2341 aggagggcgc gcagattcac cggcatgaac tagtctcctt caattggccg ccgtttcctt
     2401 cacgatttcc tcgagcgtgg tgatgccttt cttcaccttc accatgccgt ccatgcgcag
     2461 ggtgagcat
//
LOCUS       DASUBP010000013         9832 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_749996, whole genome shotgun sequence.
ACCESSION   DASUBP010000013 DASUBP010000000
VERSION     DASUBP010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9832)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9832)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9832
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_749996"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1278)
                     /locus_tag="VFU75_00525"
     CDS             complement(<1..1278)
                     /locus_tag="VFU75_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU5465341.1"
                     /translation="MSEPLPPRPPRPSLRVQIARIRAALAEVSRRSLGSIAWTPPAWV
                     AELRDKERALRAGMLADTRTFRRNAVIGGGGLILVVAAVVLWRHRPHPVLTAFQITPP
                     PGTDLTVDNPKPNPLRISFEASVAPLKSFAKPVTTGVDLSPRIPGTWRWVGDRELDFL
                     PADDWPVGQTFTVTFDKKGMFAPTVTLEEYKFDFSSAPFVAQIATAEFYQDPVDPNLK
                     RVVVHLTFSHPLDTASLSRQVTMRMEGPGGGFLSSMRSLPFTVSADKKRLTASIMSDP
                     LPIPDKDAQMRVTIGTGVKSSRGGPGTGAALEQRVVVPGMFSLSVQNAQLTLVRNERY
                     EPEQVMVLGTSADVGEKDIQQHVNAWVLPVYNPKTPADQRKYPYQWGPAEVGPDVLAA
                     SAPLTLTPIPAEREHTSTVAFKYEADPGRFVYVR"
     gene            1455..3638
                     /locus_tag="VFU75_00530"
     CDS             1455..3638
                     /locus_tag="VFU75_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465342.1"
                     /translation="MRAPPSPLLVLAFSFAVAAPAASQAPADDPDARALMAAVITRRA
                     AMLAALPPCRYDAYIKTAAFDLGAPSDSATSVLLLTETEITAYWHSPDRFQETIDARH
                     RPGDGGIGRSPATVEEIEHYQRDYVTISDASDPGAGGTRGTRLVSNARQLRTYQVPLP
                     IGADALKDYDFTRVDTVLEGSDRVLRIAVRGRGHGPRFTGTVEVRDSTYDLIGMDLLA
                     NDAVRFPGVTGLRYQQVYTDSGVTTIPSQIQLSGHLERRLSARWLPRSVAGMPMPEFP
                     RSVQFEEVATVSRFQSDTSVHPPDIAEVRRISRVNALRSDTGMWSTPGAAPLTLAEQA
                     AWAEQDSVEMHPRLVPRVVRDAEWVSDAAFGPGFGHYNRVDGLYVGLAHDWRPDPGLQ
                     FMTKLGYAFGREDWQYRAGTRITLSRPNQLWVGVTYHDESASWPSLVPTNYDATASAF
                     VNRVDPNEYYRDHGMLFSAGIRVLPYTRVEFRYDDAHQSTLDTLPSSGFRPSRFPPVP
                     NPPIADGHMRLIGGTVTFDSRQMVRTRLGEGMMSGGSWTSIALSLDLSAHDVLGSDYS
                     FRRYSMRIEHRQRWGNAGVTTVTAVGGVSTGFTPPQQYFTVGYGIQVLAAEGSAFNTL
                     SRGEFAGTRAAMVLVRHDFGRLPLIPVTFSLHGGAFWSVLRSRLAAPGDTTLFTAAHP
                     YTEAGFTLAHLTPFLTPVDFAISCTWQLSHYPTHPFRFGIGFTGF"
     gene            complement(3668..4501)
                     /locus_tag="VFU75_00535"
     CDS             complement(3668..4501)
                     /locus_tag="VFU75_00535"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HEU5465343.1"
                     /translation="MRIALWQHGLAFTLVLADMVLRAFRTQALLPMPLRRALAANTCA
                     DAASAVTPGRVGGDPVRFLAWRRAGHPPSSILMVCGSEIAADIVSLVLVGAALSFAFP
                     VVERHFIGALFRTVRDGWPWALGLVALAVTSVVVTRRFVPQHVVGVTAALREAWHEAR
                     TRPPAALIAATLLTIAALVARCAILPVLLIGVPGLSAGQVLVGSAALVYGQQLAPTPA
                     GIGAVELGAVAGLGGVVPPAALAMVVIIWRTYALVLGSLAGAALLYSYRRASTGSRVA
                     A"
     gene            complement(4498..5400)
                     /locus_tag="VFU75_00540"
     CDS             complement(4498..5400)
                     /locus_tag="VFU75_00540"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5465344.1"
                     /translation="MVPLLLLAVALAAHDTSRSFQVVVAPGESLHVAVFGRGEPVVLI
                     PGFFGSVFGFRKLIPLLEEAGYQPTVVEPLGTGFSGRPAHGDYSFIAQADQIAAVLDS
                     LRIRNAPVIAHSVGGAMALRLAYRRPDLVRSLISLEGGPTERVATPEFKRAAKYIPWI
                     KLLGGIKLIRKVVRRTLVRSSGDTTWITDGVVFGYTAGAARDLNGTLLSYLAMADSRE
                     RDRLEPHLRTIRCPVRLVIGGARHDGGVGPEEVVELARELPRFAVDSVPGAGHYLQEE
                     QPEAVLRILERVAAIPHASGASGS"
     gene            complement(5402..7006)
                     /locus_tag="VFU75_00545"
     CDS             complement(5402..7006)
                     /locus_tag="VFU75_00545"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEU5465345.1"
                     /translation="MSRIGKGAHTRLVINADDFGLSAGVNRGILAAHAAGVVTTASLL
                     ANLPAFDDAIHSAQSAPALGIGLHFNLTTGTPVSPPDQVASLVDPETGGFRGLPRLAS
                     RALRGRVDPMEVARECEAQLLRLRSRGIVITHLDAHRHVHVLPGIWEPVLRTARAAGV
                     RTVRLPFDAAANATHPRAFVERFVLRTAYRWAGGNGYRPLPDHFRGCVLYPQFRFKER
                     LLRVLDRLDAGLTELMVHPGYADPEIGTWDGYTWQREEELTALLDPEVRARLAAGDIE
                     LTTFGAATDRTSTRATTRISVIVPAFNESRYLPRLLESIDVARRRYAVDPDAVEVVVC
                     DNGSTDRTVEVAKKSGAKVIIEPRRVIAAVRNAGAGVATGDIFVFVDADSSIHPDTFT
                     RVAAALSSNRVVGGATGVTMDRWSTGIAVTFALLEAWCWVSGWDTGVLFCRRETFDEV
                     GGFPEHLLFAEDLAFYSGLRRLGRPRGQRFVRLAGVRTITSTRKFDQFGDWGWPLANA
                     KVLWCAVTRSPRERALVERHWYRVRP"
     gene            complement(7006..7752)
                     /locus_tag="VFU75_00550"
     CDS             complement(7006..7752)
                     /locus_tag="VFU75_00550"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HEU5465346.1"
                     /translation="MLRETVYQTSLNVGARLVRGIERVITWGSLVGNPAFFDADAFDW
                     VAPLEAGWRTARRELDGVMQYWDSLPNFQDISTDQRTITDDDRWKTFFLYAYGIKAEG
                     NCARCPETTRLVEAVPGMKTAFFSILAPGKHIPAHRGPFKGVIRYHLGLLVPEPAERC
                     RIRVGTEIRHWREGKSLVFDDTYEHEVWNDTSGRRAVLFLDVVRPLRFPASTFNDLIL
                     ALIKRSPFVQDGVQNYKAWEEKLERRTRAA"
     gene            complement(7857..9203)
                     /locus_tag="VFU75_00555"
     CDS             complement(7857..9203)
                     /locus_tag="VFU75_00555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5465347.1"
                     /translation="MNRLSCLLLAIPALLSAQRPDTLLSALKEPGVGGFAPFVPAAPG
                     HAVLLKPDRVFDATSEAVHNGWVVLVQGDSIAAVGPADQVRAPKGTETITLPGATLLP
                     GLIDAHSHVLLHPYNETLWNDQVLKEAPDYRVVEAVLHLRNTLMEGFTTLRDLGTEGA
                     GFADVSLKRAVLDGLIPGPRLEVSTRAIVATGSYGPGPRGFRPDLDLPQGGQAVSGVP
                     QVLEAVREQAGHGADWIKVYADYGRGPGGQAVPTFSQEELNALVSEAHSSGRPVAAHA
                     STDEGMRRAILAGVNTIEHGFGGSDSVFRMMAQHGVAYLPTLTAVEAYAEYFDGYHRG
                     QPMTASMREAASAFQMAMKNGVTIGCGSDVGVFTHGTNARELEWMVRDGMRPAQALLA
                     ATAVDAKILGMDKMIGRVVPGLRADLVAVTGDPTREIAAIERVRFVMKNGTVYRSE"
     gene            complement(9286..9456)
                     /locus_tag="VFU75_00560"
     CDS             complement(9286..9456)
                     /locus_tag="VFU75_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465348.1"
                     /translation="MTDIPNIDFTGSIPALYDEFLGPAIFTAYADDLTARISARVEGG
                     RLLETACGTRWR"
     gene            complement(9505..>9832)
                     /locus_tag="VFU75_00565"
     CDS             complement(9505..>9832)
                     /locus_tag="VFU75_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5465349.1"
                     /translation="ECGYEVLAAADGEEALELARRAGRRVEILVTDMVMPDMSGLQLA
                     DQLLRILPDLKVLCVTGYAAHHTLGTARKRGFGLLQKPFTTDELARRVRNVLDGRVSG
                     SVTAGH"
BASE COUNT         1708 a         3420 c         3147 g         1557 t
ORIGIN      
        1 acggacatag acgaagcggc ccggatcagc ctcgtacttg aacgcgacgg tggaggtgtg
       61 ttcgcgttcg gcgggaatcg gcgtcagggt caggggagcc gatgccgcca ggacatcggg
      121 gcccacctcg gcaggccccc attggtaggg atacttgcgc tggtccgccg gcgtcttggg
      181 gttgtagacc ggcagcaccc acgcgttcac gtgctgctga atgtcctttt ccccaacgtc
      241 ggcggaggtc ccgagcacca tgacctgctc cggctcatac cgttcgttcc ggaccagggt
      301 gagctgcgcg ttttgcacgg agagcgagaa cattccggga acgaccacac gctgttccaa
      361 cgccgctcca gttcccggtc ctccgcggct ggacttcacg ccagtgccga tggtcacccg
      421 catctgcgcg tctttatcgg gaatgggcaa cggatcggac atgatcgaag cggtgagccg
      481 cttcttgtcc gccgaaaccg tgaacggcag agatcgcatg ctggagagaa agccgcctcc
      541 cgggccctcc atccgcatcg tcacctgccg ggacaaggac gccgtatcga gcggatggct
      601 gaacgtcaga tggaccacca cccgcttcag gttggggtcc actggatcct ggtagaactc
      661 cgctgtcgct atttgcgcga cgaagggggc ggaggagaag tcgaacttgt attcctcgag
      721 cgtcaccgtc ggcgcgaaca ttcccttttt gtcgaatgtc accgtgaacg tctgtcccac
      781 cggccaatca tcggccggca ggaagtccag ctcccgatcc cccacccacc gccaggtccc
      841 cgggatccgg ggagaaaggt cgacgcccgt ggtgaccggt ttggcgaagg acttcagcgg
      901 ggccacggac gcttcaaacg aaatccgtaa tggattcggc ttgggattgt cgaccgtgag
      961 atcggtgccg gggggtggcg taatctgaaa cgcggtgagg acgggatgag gacggtgacg
     1021 ccacagcacc acggcggcca cgaccaggat gagaccccct ccgccgatca cggcattgcg
     1081 ccggaaggtc ctggtgtccg cgagcatacc cgcgcgcagc gcccgctcct tgtctcggag
     1141 ctctgccacc caagccggag gcgtccaggc gatgctcccc aggctgcggc gggagacttc
     1201 agcgagtgcg gcacggatcc gcgcgatctg cacgcggagg gacggacggg gtggacgggg
     1261 gggcagtggt tcgctcacga cggcctcaac ggtatgcgga agtcccgcgc gacgacgtgg
     1321 aacagcgttc ggagtgcaac gcataatatg gaccgcgtcc tcggactccg cattcgggcc
     1381 gacgagtcac gcaccgtgtc tatccgaacc cggggatgtg tccggtccaa ccatctcggg
     1441 gtattatctg ctgcatgagg gctccgccgt ctccgctcct ggtgttggcg ttttccttcg
     1501 ccgtggccgc accggccgcg tcacaagcgc cggccgacga tcccgacgca cgcgccctga
     1561 tggccgccgt cattacccgg cgcgccgcga tgctcgccgc cctcccgccc tgccgctacg
     1621 acgcgtacat caagaccgcc gccttcgacc tgggcgcccc ctccgattcg gccacatcgg
     1681 tgttgctcct gaccgagacc gaaatcaccg cgtactggca ctctcctgac cgcttccagg
     1741 agacgatcga cgcccggcac cgcccggggg acggtggcat cgggcgttca ccggctaccg
     1801 tggaagaaat cgagcactac cagcgagact acgtcaccat cagcgacgcc tcggatccag
     1861 gtgccggcgg cacccgcggc acccggctgg tctcgaacgc gcgccagctc cgcacctacc
     1921 aggtaccgct cccgatcgga gccgacgcct tgaaggacta cgatttcacc cgcgtcgata
     1981 cggtcctcga ggggagcgac cgcgtgctcc gaattgccgt tcgcggccgc gggcacggtc
     2041 cgcgatttac cggcacggtc gaagtccggg attccaccta cgacttgatc ggcatggatc
     2101 tcctcgcgaa cgacgcggtc cgcttccccg gcgtgaccgg cctgcgctac cagcaggtct
     2161 acacggactc aggggtcacc acgatcccgt cccagatcca gctcagtggc catctcgaac
     2221 gccgcctctc ggcccgctgg ttgccgcgat cggtcgccgg gatgccgatg ccggagttcc
     2281 cccgctccgt gcagttcgaa gaggtggcga cggtctcccg gttccagtcg gatacgagcg
     2341 tacatccccc ggacatcgcc gaggtacggc gcatttcccg ggtcaacgcg ctgcgctcgg
     2401 acaccggcat gtggtccaca ccgggcgccg cgccgctgac cctcgccgag caggcggcgt
     2461 gggccgagca ggattccgtg gagatgcacc cgcgactggt tccccgggtg gtacgcgatg
     2521 ccgagtgggt ctcggacgcc gcattcgggc cgggattcgg acactacaat cgggtggacg
     2581 ggctctacgt ggggctcgcg cacgattggc gccccgaccc cggtctccag ttcatgacca
     2641 agctcgggta cgcgtttggg cgtgaggact ggcagtaccg cgcgggcacg cggatcaccc
     2701 tgtcccggcc gaaccagctc tgggtgggtg tcacctatca cgacgagtcc gccagctggc
     2761 catccctggt cccgacgaac tacgacgcca ccgccagcgc cttcgtgaat cgcgtcgacc
     2821 cgaatgagta ctatcgcgat cacggcatgc tcttctcggc cggcatccgg gtcctcccgt
     2881 acacgcgggt cgagttccgt tacgacgacg cccatcaatc gacgctcgat accctgccga
     2941 gctcggggtt ccgcccgtcc cgctttccgc ccgttccgaa tcctcccatc gccgacgggc
     3001 acatgcggct catcgggggg acggtgacgt tcgattcccg gcagatggtg cggacccggc
     3061 tgggtgaggg gatgatgtcg ggtggcagct ggaccagcat cgccctgagt ctcgacctga
     3121 gcgcccacga cgtgctgggg agcgactact cgttccggcg ctactcgatg cggatcgagc
     3181 accggcaacg ctggggcaac gccggtgtca cgaccgtcac cgccgtgggt ggcgtctcga
     3241 ccgggttcac gccgccccag cagtacttca ccgtcggcta tgggatccag gtgctcgccg
     3301 cggaaggatc ggcgttcaac accctctcac gaggggaatt cgccggtacg cgcgcggcga
     3361 tggttctggt tcgacacgac ttcggacggc tcccgctcat ccccgtcacc ttcagcctgc
     3421 atggcggtgc gttctggagc gtattgcgct cgcgactggc cgcaccgggc gacacgaccc
     3481 tgttcaccgc cgcgcatccc tacaccgagg cggggttcac gctggcccac ctcaccccct
     3541 tcctcactcc cgtcgacttc gccatctcct gcacctggca gctctcgcat tacccgacgc
     3601 atcccttccg cttcggtatc gggtttacgg gattctgaat gcgtcaggag cgggcgtggc
     3661 ccgcccgtca ggccgcgacc ctcgatcccg tcgaagccct tcggtacgaa tagagcaacg
     3721 ccgcgccggc cagagatccg agcaccagcg cataggtccg ccaaatgatc accaccatcg
     3781 ccaaggcggc gggggggacc acgccaccca atccggcgac ggcgcccaac tccaccgccc
     3841 cgattccggc cggcgtgggc gccaattgct ggccgtagac cagcgccgcc gatccgacca
     3901 ggacctgccc tgcggacagc cccggaacgc cgatcaacag gaccggaagg atggcgcagc
     3961 gcgccaccaa agccgcgatg gtgagcaggg tcgccgcgat cagggccgcc ggtggccggg
     4021 tgcgcgcctc gtgccacgct tctcggagcg cggcggtcac ccccaccacg tgctgcggga
     4081 cgaaccgtct cgtcacgacc acactggtga cagccagggc aaccaatccc agcgcccagg
     4141 gccacccatc acgcaccgtc cggaagagcg ctccgatgaa atgtcgctcg acgacgggaa
     4201 aggcgaacga caacgcagcg ccgaccagta ccagagacac aatgtccgcc gcgatctccg
     4261 agccgcagac catcagaatg gacgagggcg ggtgaccggc tcgtctccag gccaggaaac
     4321 ggacggggtc gccgccgact cgtccgggag tcacggccga tgccgcgtcc gcacacgtat
     4381 tggccgcgag ggcgcgccgc aggggcatcg gcaggagcgc ctgcgtccgg aacgcacgaa
     4441 gcaccatgtc ggccaggacg agcgtgaacg cgagcccgtg ctgccacagt gcaatcctca
     4501 tgacccggac gcaccactgg catgtggtat cgcggccacc cgctcgagta tccgcagtac
     4561 ggcctcgggt tgctcctcct ggagatagtg cccggccccc gggaccgagt ccacggcgaa
     4621 tcgaggaagc tctcgtgcca gctccaccac ctcctcgggc ccgacaccgc cgtcatgtcg
     4681 cgcgccaccg atcaccaacc gcacgggaca ccggatggtc cgcagatgcg gttcgaggcg
     4741 gtcgcgctcg cgcgaatccg ccatggcgag ataggaaagc agcgtgccgt tcaggtcgcg
     4801 tgccgccccc gcggtgtacc cgaacaccac gccgtccgtg atccaggtgg tgtctccaga
     4861 cgaacgcacc agcgtgcgcc gcaccacctt ccggatcagc ttgattcctc cgagcagctt
     4921 gatccacggg atgtatttcg cggcccgttt gaactcggga gtcgcaaccc gctccgtggg
     4981 cccgccttcc agggatatga gtgaacgcac gaggtcgggc cggcgatacg ccagacgcag
     5041 cgccatcgcg ccaccgaccg agtgcgcgat caccggagcg ttgcgaattc tgagggagtc
     5101 gaggaccgcc gcgatctgat ccgcctgcgc gatgaaggag tagtccccgt gggcaggccg
     5161 tcctgaaaat ccggtcccga ggggttccac cacggtcggc tggtatcccg cctcttccag
     5221 aagcgggatc agcttgcgga agccgaagac ggatccgaag aatcccggaa tgagcacgac
     5281 gggttcgcca cggccaaaga cggccacatg caaggactca cccggtgcga ccacgacctg
     5341 gaaactgcgc gacgtatcat gggcggcgag ggccaccgcc agcagcagca gcggcaccat
     5401 gtcagggacg cacgcgatac caatgacgtt cgaccagcgc gcgctcgcgc ggggaccgcg
     5461 tcacggcgca ccaaagcacc ttggcgttgg ccaagggcca gccccaatca ccgaactggt
     5521 caaacttgcg ggtggaggta atcgtccgca cgcccgcgag ccgcacgaac ctctgcccgc
     5581 gcgggcgccc caggcgccgc aaccccgagt agaacgcgag atcctccgca aagagcaggt
     5641 gctccggaaa tcctcccacc tcgtcgaacg tttcgcggcg gcagaagagc acccccgtgt
     5701 cccatccact cacccagcac cacgcctcga ggagcgcgaa cgtcacggcg atgccggtcg
     5761 accaccgatc catggtgacg ccggttgcgc ccccgaccac gcggttggaa gagagcgcgg
     5821 cggccacccg ggtaaaggtg tcaggatgga tggacgaatc ggcatccaca aagacgaaga
     5881 tgtctcccgt cgcgacaccc gcccccgcgt tccgcacggc ggcgatgacg cggcggggct
     5941 cgatgatcac ctttgctccg ctcttcttcg cgacctcgac ggtacggtcg gtcgaaccat
     6001 tatcgcacac gaccacttcc acggcgtcgg ggtccacggc gtaccgccgt cgcgcgacat
     6061 cgatcgactc gaggagcctg ggcaaatagc gggattcgtt gaacgccggc acgatgacgg
     6121 atattcgggt cgttgcccgg gtcgatgtgc gatcggtggc ggcgccgaac gtcgtgagct
     6181 cgatgtcgcc ggccgccaga cgcgctcgta cctcaggatc caggagcgcc gtcagctcct
     6241 cttcccgttg ccaggtatag ccgtcccacg tcccgatttc gggatccgca tatccgggat
     6301 ggaccatgag ctcggtgagc cccgcgtcca gacggtccag cacccggagg agccgctcct
     6361 tgaagcggaa ctgcggatac aggacgcaac cacggaagtg atcggggagt gggcggtatc
     6421 catttccacc tgcccaccgg taggccgtcc gcaaaacgaa ccgctccacg aacgcccggg
     6481 gatgcgtggc gttggcggcg gcgtcgaacg gcaaacgcac cgtgcggacc cccgcggccc
     6541 gggccgtccg cagtaccggc tcccagatac cgggaagcac gtggacgtga cggtgggcgt
     6601 cgagatgagt gatcacaatc ccgcgcgagc gaagccgcag cagctgcgcc tcgcactccc
     6661 gcgcgacctc catcgggtcc acccgccctc gcaacgcccg cgacgcgagc cgggggaggc
     6721 cgcgaaaccc gcccgtctcc gggtcgacga ggctggccac ctgatcgggc ggcgataccg
     6781 gtgtgcccgt cgtcagattg aagtggagtc cgataccgag ggccggggcg gactgcgccg
     6841 agtggattgc gtcgtcgaag gccggcagat tggcgagcag cgaggccgtg gtcaccacgc
     6901 ccgcggcatg agcggcgagg atcccccggt tgaccccggc cgagaggccg aagtcgtcgg
     6961 cgttgatcac cagacgggtg tgggcaccct tcccgatccg gctcactacg cagcccgggt
     7021 tcgccgctcg agtttctcct cccacgcctt gtagttctgc accccatcct gcacgaaggg
     7081 gctgcgcttg atcagcgcga ggatgaggtc attgaacgtg ctggccggga accgcagggg
     7141 acggaccacg tcgagaaaga ggacggcgcg ccggccgctg gtgtcgttcc agacctcgtg
     7201 ttcgtaggtg tcgtcgaaca ccaggctctt cccctcccgc cagtgccgga tctcggtgcc
     7261 gacgcgaatc cggcaccgct ccgccggttc gggaacgagc aaacccaggt gataccggat
     7321 gacgcccttg aaggggcccc ggtgagccgg gatgtgcttg cctggtgcga ggatagagaa
     7381 aaacgccgtc ttcatcccgg gaacggcctc gactaaccgg gtcgtttccg gacagcgggc
     7441 acagttcccc tcggccttga tcccgtaggc gtagagaaag aacgtcttcc atcggtcgtc
     7501 atcggtgatg gtgcgctgat cggtggagat gtcctggaag ttcgggaggg agtcccaata
     7561 ctgcatcacg ccgtcgagct cccggcgggc ggtccgccag ccggcctcga ggggtgccac
     7621 ccaatcgaac gcgtcggcgt cgaagaaggc ggggttcccc accagcgaac cccaggtgat
     7681 gacacgctcg atccctctga ccaatcgagc gccgacgttc aatgaagtct ggtataccgt
     7741 ttcgcgaagc acgcccactc ggacactcgg ctgagaaggg ttcggattgc cttatccgag
     7801 acccctcgac cccacattcg tcacggggtg caacgcgatt tcgccgtcgc gatatgttac
     7861 tccgaccgat agaccgttcc atttttcatc acgaaccgga cccgctcgat cgccgcgatc
     7921 tccctggtgg gatcaccggt gaccgcgacc agatccgccc gcaacccggg caccacccgg
     7981 ccgatcatct tgtccatccc gaggatcttc gcgtccaccg ccgtcgccgc gagaagcgcc
     8041 tgggcaggcc gcatcccgtc gcggaccatc cactccaatt cgcgggcgtt ggtgccgtgg
     8101 gtgaacacac ccacgtcact gccgcagccg atggtgacgc cgttcttcat ggccatctga
     8161 aaggcactcg ccgcctcgcg catgctcgcc gtcatcggct gaccccgatg gtagccatcg
     8221 aagtattcgg cataggcttc gaccgcggtg agggtcggca ggtacgccac gccgtgctgg
     8281 gccatcatcc ggaacacgga gtcgctcccg ccgaatccgt gctcgatggt gttcacaccc
     8341 gcgagaatcg cccggcgcat gccttcatcc gtgctcgcat gggccgcgac gggccggccc
     8401 gatgagtggg cctccgacac gagcgcgttg agctcttcct gactgaacgt ggggacggcc
     8461 tgtccgcccg gccctcggcc gtagtccgcg tagaccttga tccagtcggc accatgccct
     8521 gcctgctccc ggacggcctc gagcacctgg ggcacgccgc tgaccgcctg accgccttgc
     8581 ggcagatcga gatcggggcg gaacccgcgg ggtccgggcc catagctgcc cgtcgcgacg
     8641 atcgcccgcg tggacacctc gagccgcggt ccggggatca gtccgtcgag gacggcccgt
     8701 ttgagggaca cgtccgcgaa tccggcgcct tcggtgccca ggtcacggag cgtcgtgaag
     8761 ccttccatca aggtgttgcg caaatggaga acggcttcga ccacgcggta gtccggagcc
     8821 tccttcagga cctgatcgtt ccagagcgtc tcgttgtacg gatggaggag cacgtgcgag
     8881 tgcgcgtcga tcagtccggg aaggagcgtc gcaccgggga gcgtgatggt ctccgtgcct
     8941 ttgggcgcgc gcacttgatc ggcgggaccg accgccgcga tgctatcgcc ttgcaccagg
     9001 acgacccatc cgttgtgtac cgcttcgctg gtcgcatcga acacccgatc gggcttgagg
     9061 agcaccgcat gtcccggggc agcgggcacg aagggagcaa acccaccgac gcccggttcc
     9121 ttgagcgcag acagcagcgt atccggacgt tgggcggaca gcagtgccgg aatggccagc
     9181 aagagacaag acaaccggtt catgacgttc tcctctagtg agctttggcg gtgccgacga
     9241 ccgccaccat cgtcgaacgg aagggggccc cgcccccttc tttcgtcagc gccatcgtgt
     9301 gccgcaggcc gtttccagca ggcgaccccc ttccacgcgc gcactgatgc gagccgtgag
     9361 gtcgtcggcg taggccgtga agatggccgg acccaggaat tcatcgtaca gggccggaat
     9421 ggatccggtg aaatcgatgt tggggatatc ggtcacgcca ggaagctatg ccccaggact
     9481 cggtgcgcga gactgtggca cccgtcaatg ccctgcggtc acgctccccg agacccgccc
     9541 atccagaacg tttctgaccc gccgggcgag ctcatcggtc gtgaaaggct tctgcaacaa
     9601 gccgaacccc cgcttccgcg cggtccccag ggtgtggtgc gccgcatatc cggtcacgca
     9661 aagcactttc aggtccggca aaatccggag aagctggtcg gccagctgca gcccgctcat
     9721 gtcgggcatc accatgtcgg tcacgagaat ctccacccgg cgaccggcgc gccgcgcgag
     9781 ctcgagcgcc tcttccccgt ccgcggccgc cagcacttcg tagccgcact cc
//
LOCUS       DASUBP010000014         3066 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_218495, whole genome shotgun sequence.
ACCESSION   DASUBP010000014 DASUBP010000000
VERSION     DASUBP010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3066)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3066)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3066
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_218495"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            174..938
                     /locus_tag="VFU75_00570"
     CDS             174..938
                     /locus_tag="VFU75_00570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5465350.1"
                     /translation="MIQLQGLTKHYGSFVAVDHIDLTVDKGLLYGCLGPNGAGKTTTL
                     RMIAGILRPTDGRVLLDGDDVHRAPLVAKQKLGFIPDRPFVYDKLTGAEFLRFVAGLY
                     GQDGDVVERRIAELLDVFELSSWKDELVESYSHGMRQKLIISSALIHRPAVIVVDEPM
                     VGLDPKAARLLKDIFRQFVDRGGTVLMSTHTLEVAEAMCDRIAIVQHGKIVAEGTVND
                     IRTQHQAGDASLEELFLKLTGGAQAKELAEVLGGGK"
     gene            935..2626
                     /locus_tag="VFU75_00575"
     CDS             935..2626
                     /locus_tag="VFU75_00575"
                     /inference="COORDINATES: protein motif:HMM:NF028259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465351.1"
                     /translation="MNQPSLAHVLQPKWRTALQRMRQERSQGGGGKAVLLTAVGITFF
                     AGVFVVLYVILRYFRGVEELGPLLASKMLGLALLSFISILVLSNVITALSSFFLAKDL
                     DLLVSAPVDWLRLYLAKLLETAIHSSWMVALMAVPLLTAYGVIYHGGWFFALYAILVM
                     VPLLILPAVLGSALTLILVNIFPARRTRDLLSIIALGAGAGVILLFRMIRPEQLARPE
                     GFRNLLEFIAVLRTPTSPFMPSEWATRAIMGYLHGPFDNLPLILLWSTAAAFVTLGAM
                     LHAQLYATGYTKAQEGAERFIRGGFWKWSLGAVMSPLSVAKREFVLKDIRLFFRDTTQ
                     WSQLILLGVLVVVYIFNIKVLPLHRGEQVGFFSVTLVSFLNLGLAGFVLTSIAARFIF
                     PAISLEGRQMWLLRSSPLDLKTLLWSKYWVGTVPLLVLALILTGVTNWLLQASPFMMT
                     LSLVTIVALTLAIGALALGYGTLYPQFETENAAQIPTSFGGLVFMMTTIALLAVVIGG
                     LSQIVYPYVRSVYEGHPVVVTPWMIISTVAVALVCVAATVIPLWLGLKKMEQFEF"
     gene            2647..>3066
                     /locus_tag="VFU75_00580"
     CDS             2647..>3066
                     /locus_tag="VFU75_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5465352.1"
                     /translation="MPCVLTAQSPQLVNIGSHRLEVVRAGTGSPTIVFEAGLGDTWED
                     WKPVWQQASQLSSVVLYSRSGLGQSEPGPGDHSARTEMTELHQLLDSLHIQRPIVLVA
                     RSYGGLLSRLYVSMYPHDVAGLVLVDGTHEQQVKRWGQ"
BASE COUNT          465 a          995 c          985 g          621 t
ORIGIN      
        1 gaccgcttct actattacga gtgggcggag gtgtggctgg ggcaatgggt accggtcgac
       61 gcctacaatg accagttccc cgcggatgct tcccatctgc ggttgatgat cgggatgccc
      121 gggcgtcgcg ccgtcatgac gacccggctg gggacgctca cgctcgaggc gcaatgattc
      181 agctccaagg cctgaccaag cactacggct ccttcgtcgc ggtggaccac atcgacctga
      241 cggtggacaa gggactgctc tacggctgcc tcggcccgaa tggcgcgggc aagaccacca
      301 cgctccggat gatcgctggc attctgcgcc ccaccgatgg ccgggtcctg ctcgacggcg
      361 acgacgtgca ccgtgcgccc ctcgtcgcca agcagaaact cggcttcatt cccgaccggc
      421 ctttcgtcta cgacaagctc accggcgccg agttcctccg gttcgtggcg gggctctacg
      481 gccaggacgg cgacgtcgtg gagcgccgca tcgcggagct gctcgacgtc ttcgagctgt
      541 cgagctggaa ggacgagctg gtcgagtcgt acagccacgg catgcggcag aagctcatca
      601 tatcgagcgc gctgattcac cgcccggcgg tgatcgtagt agacgagccc atggtgggac
      661 tcgatccgaa ggcggcgcgc ctgctcaagg acatcttccg tcaattcgtg gaccgcggcg
      721 gcaccgtcct catgagcacg cacacgctcg aagtcgccga agcgatgtgc gaccggatcg
      781 cgatcgtcca gcacggcaag atcgtggcgg agggcaccgt gaacgacatc cggacccagc
      841 accaggccgg tgacgcgagc ctcgaagagc tgttcctcaa gctcaccggt ggcgcacagg
      901 cgaaggaatt ggccgaggtg ctgggggggg gcaagtgaat cagccgtcgc tcgcgcacgt
      961 cctgcagccc aagtggcgga ccgcgctgca acggatgcgc caggagcggt cacagggggg
     1021 cgggggcaag gccgtgcttc tcaccgcggt ggggatcacg tttttcgccg gcgtgttcgt
     1081 cgtgctctac gttattctcc gctacttccg cggcgtcgaa gagctcgggc ccctcttggc
     1141 gagcaagatg ctgggtctcg ccttgctctc cttcatctcc attctggtcc tctcgaacgt
     1201 catcacggcg ctctcgagct tcttcctcgc gaaggatctc gacctgctgg tctcggctcc
     1261 ggtcgactgg ctccgcctgt atctcgcgaa gctcctggag accgccatcc attcgtcgtg
     1321 gatggtggcg ctgatggcgg tgcccctgct gacggcatac ggggtgatct accacggggg
     1381 ctggttcttt gcgctctacg ccatcctcgt gatggtgcca ctgctgatcc tcccggccgt
     1441 cctcggctcc gcgctcacgt tgatcctggt caatattttc ccggcccgcc ggacccgcga
     1501 tctgctctcg attatcgccc tcggcgccgg tgcgggcgtc attctcctgt tccggatgat
     1561 ccggccggag cagctggccc ggccggaagg cttccggaac ctgctcgagt tcatcgccgt
     1621 gttacgaacc ccgacctccc cgttcatgcc gagcgagtgg gcgacgcggg caatcatggg
     1681 gtacctccac gggccgttcg acaacctccc gctcatcctg ctctggagca cggcggccgc
     1741 gtttgtgacc ctgggggcga tgctgcacgc gcagctctat gccaccgggt atacgaaggc
     1801 gcaggagggt gcggagcgct tcattcgggg cgggttctgg aagtggtccc tgggggcggt
     1861 gatgtcaccc ctttcagtgg cgaaacggga attcgtgctg aaggacatcc ggctcttttt
     1921 ccgggacacc acccagtgga gccagctgat cctgctcggc gtcttggtgg tggtctacat
     1981 cttcaacatc aaagtcctgc ccctgcaccg gggcgagcag gtgggattct tctcggtgac
     2041 gctcgtgtcc ttccttaatt tgggactggc gggcttcgtg ctcacgtcga tcgcggcgcg
     2101 cttcatcttc ccggccattt ccctcgaggg ccggcagatg tggttgttgc ggtcgtcgcc
     2161 actcgacctg aaaaccctgt tgtggtccaa gtattgggtg ggcacggtgc cgctgctcgt
     2221 gctggcgttg attctcaccg gcgtgacgaa ctggcttctg caggcgtcgc cgttcatgat
     2281 gacgctgagt cttgtcacga tcgtggcgct gacgctggcg atcggcgcgc tcgccctcgg
     2341 gtacggcacg ttgtatcctc agttcgagac cgagaacgcg gcccagatcc cgacgtcatt
     2401 cggcggattg gtgttcatga tgaccacgat tgccctgttg gcggtggtca tcggcgggtt
     2461 gtcgcagatc gtgtacccct acgtccggtc ggtctacgag gggcatccgg tggtggtcac
     2521 gccctggatg atcatcagca cggtcgcggt cgccctggtc tgtgtcgccg cgacggtgat
     2581 tcccctctgg ctaggtctca agaaaatgga gcagttcgaa ttctgaaact cgccctcttc
     2641 ctcgccgtgc cctgcgtcct caccgcccag agtccgcagc tggtcaacat cggctcgcac
     2701 cggctcgaag tcgtccgcgc cggcaccggt tcccccacca tcgtgtttga agccggcctc
     2761 ggcgacacct gggaggactg gaagccggtc tggcagcagg cgtcccagct atcgagtgtg
     2821 gtcctgtact cgcgctccgg gctcggccaa tccgaaccgg gtcccggcga tcactcggcg
     2881 cgtaccgaga tgacggagct ccatcagctc ctcgacagcc tgcacatcca gcggccgatc
     2941 gttctcgtcg cccgctcgta tggcggtctg ctctcgcgat tgtacgtctc gatgtacccg
     3001 cacgatgtcg cgggtctggt gctggtggac ggcacccacg agcaacaggt caagcgatgg
     3061 ggacag
//
LOCUS       DASUBP010000015         3939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_341130, whole genome shotgun sequence.
ACCESSION   DASUBP010000015 DASUBP010000000
VERSION     DASUBP010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_341130"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..535
                     /locus_tag="VFU75_00585"
     CDS             <1..535
                     /locus_tag="VFU75_00585"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU5465353.1"
                     /translation="DPIAVVADRAGLGWMSTLIKLGAIAGLSSVILVMLLGQSRVFWS
                     MSKDGLLPQVVNKIHPKFQTPYITSIVTGIFVAIFSAILTVREAASLVSIGTLLAFVI
                     VSIGIMVLRVREPNLHRPFKAPWIWFTAPMGALSAAYLMKSLPWATWYRLIIWFAIGM
                     VFYFVYGVRHSRLRKTS"
     gene            661..1677
                     /locus_tag="VFU75_00590"
     CDS             661..1677
                     /locus_tag="VFU75_00590"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEU5465354.1"
                     /translation="MTSNAIDVPPERVSAAREMARLFSPNTRVVLTTHVNSDGDGLGS
                     EVGLWHLLHRRGCIVSITNPTPIPDRLRFLVPEGADASANAAKEIAKADAIVVLDISD
                     LGRLGDLGAAVRESGVPVACIDHHVSPGSLPDGPRLVAPEATATAELVHDLAVALGWE
                     IPADAARALYVGLLTDTGGFRFSNTTPRCLRIAGSLLERGLDPESIYEQVYASAPEGR
                     VRLLGEVLTTLVVEPEIGLAWVTVPPGAIEKHGATADDLDGIVEHPRSIAGVRMALLF
                     RPIAGGKVKVSFRSMGSVNVAELAHQFGGGGHAKASGASVEGPLADAQVKVLEAARRH
                     LNGR"
     gene            1714..2352
                     /locus_tag="VFU75_00595"
     CDS             1714..2352
                     /locus_tag="VFU75_00595"
                     /inference="COORDINATES: protein motif:HMM:NF018178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HEU5465355.1"
                     /translation="MRTITLCIMATALAAAPATAQTGKWTPLFDGSLSGWEHVGPGKF
                     TVDSGLAMTHGGMGLLWYSKRKIGNEVIRVVFWPTDSTSNSGVFIRIPEKPTEPWMPV
                     NKGYEVQIDNAEDDYHVSGVLYSLTKALARPGKARQWNTLEITLDGPRTTVRLNGEVV
                     TDYTEGQPVPPKKQTWEPDRGRRPDLGYIGLQNHSDADTVYFKEVSVRPLHP"
     gene            2364..2792
                     /locus_tag="VFU75_00600"
     CDS             2364..2792
                     /locus_tag="VFU75_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465356.1"
                     /translation="MRRLAFGVALVIAVAACDPSTDVGILGHGIIGGGGPGGAATKLV
                     FTQQPANTQATAAMATAVVVEAQDPLGNVDTSYNGVVTLALSSNPDTAVLGGNFSVSA
                     IFGVATFSNLTVSKADTGYVLTAGSGALTAGFSTKFTITP"
     gene            complement(2844..>3939)
                     /locus_tag="VFU75_00605"
     CDS             complement(2844..>3939)
                     /locus_tag="VFU75_00605"
                     /inference="COORDINATES: protein motif:HMM:NF027814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase family 27 protein"
                     /protein_id="HEU5465357.1"
                     /translation="PLDNGLARTPPMGWNSWNHFACDVSDRLIRETADAMVSSGMKDA
                     GYRYVIIDDCWQVARAKDGTIVADSVRFPNGIKALADYVHSKGLKFGIYTDAGTETCQ
                     GRPGTLGHEAQDARTYASWGVDYVKEDWCHASGLDAPTQYAKFRDGLAAAGRPIVLSL
                     CEWGYNQPWNWAPKIGNLWRTTDDISDSWSSMLGNLDQSAQHPTAAGPGAWNDPDMLE
                     VGNGGMTDDEYRAHFSLWAIEAAPLIAGNDLRSMSAETKTILENAEVIAVDQDSLGVQ
                     GVNVWEPTPEIQVWAKPLADGSAAAVLLNRAGVSTAITAPFHRLGLRADSGAVRDLWA
                     HADKGHFRAEYTVTVPPHAVVMIRVTPVKH"
BASE COUNT          660 a         1293 c         1276 g          710 t
ORIGIN      
        1 cgacccgatc gcggtcgtgg ccgaccgggc ggggttgggc tggatgtcca cgctgatcaa
       61 gcttggcgcc atcgccggat tgtcgtccgt cattctcgtc atgctcctcg ggcagtcgcg
      121 cgtgttctgg agcatgtcga aggatgggct gctgccacag gtcgtgaaca agatccatcc
      181 gaagttccag acaccgtaca tcacgtcgat cgtcactgga attttcgtcg ccatcttttc
      241 ggcgatcctg acggtgcgcg aagcggcgag ccttgtctcc atcgggacgc tgctggcgtt
      301 cgtgatcgta tcgattggca tcatggtcct gcgggtgcgg gagcccaatc tgcaccgtcc
      361 gttcaaagcc ccctggatct ggtttaccgc acccatgggc gcgctgtcgg ccgcctatct
      421 catgaagtca cttccgtggg cgacctggta ccgcttgatc atctggttcg cgatcggcat
      481 ggtcttctac ttcgtctacg gcgtcaggca ttcgagactg agaaagacca gctaaccaag
      541 agcgtcaagg agaaccgtcg aggggagacg tcgaggtaca acgtttcccc tcgacgtttt
      601 tcctcgacga tcttcctcga cgatttccct tgacgcctct cctcgacgta gttttcagac
      661 atgacttcca acgcaattga cgtccctccc gaacgcgtct ccgcggcgcg ggagatggcg
      721 cgcctcttct cccccaacac tcgcgtcgtc ctgacgaccc acgtcaattc ggacggtgac
      781 ggactgggga gcgaggtcgg gctctggcac ctcctccatc ggcgcggctg cattgtctcc
      841 atcaccaatc ccacgcccat ccccgatcgg ctcaggttcc tcgttcccga aggcgccgac
      901 gcgagcgcga acgccgcgaa agaaatcgcc aaggcggatg cgatcgtggt gctcgacatc
      961 tcggacctcg ggcggctggg cgatctgggg gccgcggtcc gggagtccgg cgtacccgtc
     1021 gcctgcatcg accatcacgt gagtccgggg tcgctgcccg atggcccgag gctcgtcgcc
     1081 cccgaggcga cggcgacggc ggagctggtg catgacctcg ccgtggcgct gggatgggaa
     1141 atcccggccg acgcggcgcg cgcgctctac gtggggctcc tgaccgatac cggcggattc
     1201 cggttctcga acacgacacc gcggtgccta cggatcgccg ggtcgctgct cgagcgcggg
     1261 ctcgaccccg aatcgatcta cgagcaggtg tacgcgagcg cgcccgaagg acgggttcgg
     1321 ctcctgggcg aagtcctgac cacgctggtg gtcgagccgg agatcgggct ggcctgggtc
     1381 accgtgcccc ccggtgccat cgagaaacat ggcgccaccg ccgacgatct cgacgggatc
     1441 gtggagcacc cccggtccat cgccggggta cgaatggcgt tgctcttccg gccgatcgcc
     1501 gggggcaagg tcaaggtttc attccgctcc atgggaagcg tcaacgttgc ggagcttgca
     1561 catcaattcg ggggcggagg tcatgcgaaa gcgtcggggg catccgtgga ggggcccttg
     1621 gcagacgccc aggtcaaggt tctggaggcc gcccgccggc atctcaacgg acgctgacag
     1681 cgtccggctc acaccatccg tatcttgagg atcatgagaa ccatcactct ttgcatcatg
     1741 gcgaccgccc tcgcggcggc tcccgccacc gcgcagaccg ggaagtggac gcccctgttc
     1801 gacgggagcc tgtcgggatg ggaacacgtc gggcccggga agttcaccgt cgactccggc
     1861 ctggcgatga cccacggcgg catgggactg ctgtggtact ccaagcgaaa gatcggcaat
     1921 gaagtgattc gcgttgtctt ctggccgacc gactccacct ccaactcggg agtgttcatc
     1981 cgcattcctg agaagccgac cgaaccctgg atgccggtca acaagggcta tgaagtgcag
     2041 atcgacaacg ccgaagacga ctatcacgtc tccggggtgc tctactcgct gaccaaggcg
     2101 ctggcgcggc ccggaaaggc gcggcagtgg aataccctcg agatcaccct cgacggtccg
     2161 cgcaccacgg tccggctcaa cggggaagtg gtgaccgact acaccgaagg tcagccggta
     2221 ccacccaaga agcagacgtg ggagccggac cgtggacggc gccccgatct ggggtacatc
     2281 gggctgcaga accacagtga cgcggatacc gtgtacttca aggaagtcag cgtgcgcccg
     2341 ctgcatccct agaggaggta tcgatgcgca gactcgcgtt cggagtggcg ttggtgatcg
     2401 cggtcgcagc ctgcgacccg tcgacggatg tcgggatcct ggggcacggt atcatcggcg
     2461 gcgggggtcc cggcggcgcg gcgaccaagc tggtcttcac tcagcagcct gcgaacaccc
     2521 aggcgaccgc ggcaatggcc accgcggtgg tggtcgaggc gcaggatcca ctggggaacg
     2581 tcgacaccag ttacaacggc gtggtcaccc tcgccctgtc gtcgaacccc gacacggccg
     2641 tcctgggcgg taacttcagc gtcagcgcga ttttcggggt ggccaccttc tcgaacctca
     2701 ccgtgagcaa ggccgacacc ggctacgtgt tgaccgccgg ctcgggcgcc ctcaccgcgg
     2761 gcttctcgac caagttcacc atcacgccct agggccagac ggtcagacgg ctagacggct
     2821 agtttgtcta accgtctaac cgtctagtgc ttcaccggcg tgacccggat catcaccacc
     2881 gcatgcggcg gtaccgtgac cgtgtactcc gcgcggaagt gtcccttgtc ggcgtgagcc
     2941 cagagatccc gcacggcccc tgagtcggcc cgcagtccca gccgatgaaa tggggctgtg
     3001 atcgccgtgg acactcccgc ccggttcaac aggaccgccg ccgccgagcc gtccgcgaga
     3061 ggtttggccc acacctgaat ctccggcgtc ggctcccaga cattcacgcc ctgcacgccc
     3121 agcgaatcct gatcgacggc gatcacctcg gcattctcga ggatggtctt cgtttcggcg
     3181 ctcatggaac ggaggtcgtt gcccgcgatc aggggcgcgg cctcgatggc ccacagcgag
     3241 aagtgcgcgc ggtactcatc gtccgtcatt cccccattgc ccacttccag catgtccggg
     3301 tcgttccacg ctccgggacc cgccgccgtc gggtgttgag ccgactggtc gagattgccg
     3361 agcatggagc tccaggaatc ggagatgtcg tcggtggtgc gccacaggtt cccgattttg
     3421 ggagcccagt tccacggctg gttgtagccc cactcgcaaa gcgagaggac gatcgggcgt
     3481 ccggcggccg ccaggccatc ccggaacttg gcgtattggg tcggcgcatc gaggcccgac
     3541 gcgtggcacc agtcttcctt cacgtagtcg acgccccacg aggcgtacgt cctggcgtcc
     3601 tgggcttcgt ggccgagcgt gccggggcgg ccctggcagg tctcggttcc ggcatcggtg
     3661 tagatgccga acttgagacc cttcgagtgg acgtagtcgg cgagcgcctt gatgccgttg
     3721 ggaaagcgca cggaatcggc cacgatggtg ccgtctttcg cgcgggccac ctgccagcag
     3781 tcgtcgatga tcacgtagcg gtagcccgcg tctttcatgc cgctcgagac catggcgtcc
     3841 gcggtttcgc ggatgagccg gtccgagacg tcgcaggcga agtggttcca gctgttccag
     3901 cccatgggcg gcgtacgggc cagaccgttg tcgagcgga
//
LOCUS       DASUBP010000016        10488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_654593, whole genome shotgun sequence.
ACCESSION   DASUBP010000016 DASUBP010000000
VERSION     DASUBP010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 10488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10488
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654593"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1616)
                     /locus_tag="VFU75_00610"
     CDS             complement(<1..1616)
                     /locus_tag="VFU75_00610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HEU5465358.1"
                     /translation="MGAETSFSHRGRAAALAKMTDAPVDLLVIGGGITGAAIARDAAL
                     RGLSVGLVGKGDFGGGTSSHSSRLIHGGLRYLEHGDFRLVFEASRERRVLLNIAPHLV
                     RPLPFLFPAYRGSRVPGWKIFAALWLYDLLASFRNVHTHKWLSRKAARRMEPGLREKG
                     LTGAGLYWDAQTDDARLTLANLRAAVLAGAQVANYVEVAALSKPDGTVKGVAVQDQLT
                     GQTATIQGKVVVNATGPWVDGLRRMDDPNAPALLRPTKGVHVVVPRSRIGHTNAITLL
                     SPVDGRVMFVLPWDDVSYIGTTDTDDSVSPDEVRASGDDVIYLLRSANALFPQARLAP
                     KDVISTWAGLRPLLAPEKSATPAQVSREHRIVESASGLMTIAGGKLTTHRVMGRDVVD
                     RVAQKLHDADGRPVPPRAATDHLVLPGGDIADLEVMVESARARDIAEPVARHLVHAYG
                     SESAALVNLIERDRTLGRPILDGRPEVWAEVVHAVEREMAMRLSDVLIRRTHLFYEDP
                     AHGSAAAPPVARRMADLLGWDRTQETEEIA"
     gene            1695..2948
                     /locus_tag="VFU75_00615"
     CDS             1695..2948
                     /locus_tag="VFU75_00615"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEU5465359.1"
                     /translation="MPQGDRRVAIVAGCRTPFAKSGTVFRDVLAVDLARHAGRELLER
                     TNLDPREVDEVIFGQVVASPLVPNVAREVSLLPQLPKEIPAYTVNRACASSGQAVTNA
                     ADQIVRGHADVILAGGVESLSDVPILHTHRMAQILTELSKAKSLGARLSTLAKIRPRD
                     LIPVSPAISEPSTGETMGQSAEKMAKENGITREAQDQWALRSHKLAAAGTADGRLKAE
                     IVPWLPDGGGAPITADNGIRTDTTLEQMAKLKPVFDRRYGSVTAANSSPLTDGASAVL
                     LMSEEAARALGYKPLAFIRSYAVAAVDPGWQLLQGPLWAVPKALERAGIPWSSLGLLE
                     VHEAFAAQVLSNFKGWSEKGWNVNPDIINVMGGSIAIGHPFGATGGRVLTTLANEMNR
                     RDVQFGLVSICAQGGMAFACVLERV"
     gene            2948..5077
                     /gene="fadJ"
                     /locus_tag="VFU75_00620"
     CDS             2948..5077
                     /gene="fadJ"
                     /locus_tag="VFU75_00620"
                     /EC_number="1.1.1.35"
                     /EC_number="4.2.1.17"
                     /EC_number="5.1.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF008363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003857 - 3-hydroxyacyl-CoA dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0008692 - 3-hydroxybutyryl-CoA epimerase
                     activity [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HEU5465360.1"
                     /translation="MAGPALTWEIADGIAIVTFDTPGSSVNVISAPVKQEVIRAFDTI
                     KADERVKAVAFLSGKRDNFIAGADIEEFVKLQTASEAEQLSADGQAMLGKVAEFPKPV
                     VVGIHGACLGGGLEFSLCCTYRIATDHPRTSIGLPEIKLGILPGAGGCQRLPRLIGVR
                     AALDIILAGKTVPARKALQVGIIDEMVPQSILRRQTLEIAKKLAGGWRPHRRRPGGLM
                     GFLLDGNPLGRMLVFNLARKSVEKETHGNYPAPLAALDAVEFGLKHGMTAGLAHEARG
                     FGRLAVTDVSRKLVQIFFADSALKKDLGQENPPPPVEVRRLGVVGSGFMGSGIAGTAV
                     VQAGVDARLKDADLGHVSKGVAAARSVIEDRYKRRRITRFDRIRLLALVSGDAAFTGF
                     GRAELVIEAVFEDLKVKQQVLKEVEAAIQPSAVFATNTSTIPISDIASASSRPGQVIG
                     MHFFSPVPRMPLLEIIPGKATAPGTVSTAVAFGRRMGKTPVVVKDSPGFWVNRILAPY
                     MCEAAYVLLGGTGIDEIDRALVQFGFPVGPLTLLDEVGLDVAVHASEVLFKAFGERMK
                     PAPVLEAFIKDQRLGRKSSKGFYKYEKGKKAGVDETVYTLAGVHPNGGPRAAEIVQRL
                     TLSMVNEAARAFSEGVVRSPRDGDIAAIYGFGFPPFRGGPLRYADDLGAARIVDDLER
                     LTERLGMRFTPCDALKDLARSGGKFYS"
     gene            5101..6033
                     /locus_tag="VFU75_00625"
     CDS             5101..6033
                     /locus_tag="VFU75_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HEU5465361.1"
                     /translation="MLRAGLLWLSEQPQVFRFVRSSRLAQKFASRFVAGETVDTAIAV
                     LKDLNAANLSVSLDLLGESVSSPEEAKGAQHNYLELLDRIQSAGGNGNVSVKLTQMGL
                     DIDEALCVANLRSIIGRAKQYHSFVRLDMEGSAYTERTLSLFKRTFYPEFGQSTGVVL
                     QSYLRRTAKDVDEMIGLAARVRLCKGAYKEPATVAFPNKADVDANYVACMEQLMERGN
                     YPGLATHDEKIIEHAKEFAKKKGIGSERFEFQMLYGVRRDLQMKLRKEGWNLRVYVPY
                     GTHWYPYLMRRLAERPANLWFILGNVIRESLRRS"
     gene            6030..6347
                     /locus_tag="VFU75_00630"
     CDS             6030..6347
                     /locus_tag="VFU75_00630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465362.1"
                     /translation="MSSPDGTLGGYQRVHQRAPAFGGSDGQAYTVSTFVDDDPDPAGR
                     YGAALLFIRWSEAGERPVGHVETDYLAFGPDPDAALALVLDLSLEDVKAHLDRLLATP
                     MAE"
     gene            6344..7270
                     /gene="rsgA"
                     /locus_tag="VFU75_00635"
     CDS             6344..7270
                     /gene="rsgA"
                     /locus_tag="VFU75_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEU5465363.1"
                     /translation="MTSGVVLARSGSTYRVHVEGREIVATLRGKLKHKDDDRVVVGDV
                     VEMDMESGDSGAITGIKERRSVLQRRAAGQGAGRSQPIAANVDQVLVVASARDPELTP
                     RMIDRFLVIAEANHLPAFVVVNKAELDREAGPALADRYRAAEYPVLLTSVKVPEGLGE
                     LEERLRGRETVLAGQSGVGKSSLLNAIEPGLKLRIGAISEKWRTGKHTTTAAELIPLK
                     AGGYVVDTPGMREVGTWGIEPAELGSCFPEFRQFLGQCRFDNCRHLNEPSCAVRRGAD
                     AGAFPKDRLLSYERIYEEINVPSWSSGRRRGS"
     gene            complement(7227..7547)
                     /locus_tag="VFU75_00640"
     CDS             complement(7227..7547)
                     /locus_tag="VFU75_00640"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEU5465364.1"
                     /translation="MSFGEAQAAVDQWISQYKEGYFSPLTNLARLTEEVGELAREINH
                     RFGQKTKKAEEPEGNLALELGDILFVVICLANSQHIDLDQAFAATLAKVRTRDADRWT
                     RKER"
     gene            7562..8800
                     /locus_tag="VFU75_00645"
     CDS             7562..8800
                     /locus_tag="VFU75_00645"
                     /inference="COORDINATES: protein motif:HMM:NF018004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HEU5465365.1"
                     /translation="MTAAKPLVMPFRGERYARPSLDAVLAPPYDVISPDARKTLAARD
                     PHNIVHLILPEAPVAEEKYAQAARLLREWRKTGVLAEDPGPSVYVVAQDYTLPSGEHR
                     TRVGMLAAVSAEPYETRRIRPHEKTHAGPKADRLALLKETQTGLESIFLLAPDADRGL
                     TKALLEVTKTKPVAQGTLDGVGHRLWVVTGKAGDQLAERASAGALYIADGHHRYETAV
                     AYAKENPKADRVMAFIVSAHDPGLVVLPTHRVIYGAGRDVSHLIEQWKLWFDVTPGGD
                     VTDVVSHLAKAGASHPACFVALPKDGHTLVLRANAPLDDVPGLGTSPALRSLDVAVIE
                     PLVVKQILGAGTSTPTLTYTPDARVALDAVKKGHAAASVLLNPTKVSQVVAVADAGEV
                     MPQKSTYFVPKVPAGLVLKP"
     gene            8797..9063
                     /locus_tag="VFU75_00650"
     CDS             8797..9063
                     /locus_tag="VFU75_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465366.1"
                     /translation="MSAGRPREKILENLESVYREAYDRAKTAGDERRMADLDAAYQRE
                     QLLLEVLLDIRDGLTSRSAPAAPAPSGPDPIAVVDTIRRISQLR"
     gene            9076..>10488
                     /locus_tag="VFU75_00655"
     CDS             9076..>10488
                     /locus_tag="VFU75_00655"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU5465367.1"
                     /translation="MRRLSLLLLLPVAACGQKADLVINHGMVWTGLTSSGPQPGGVAI
                     QGDKIVAVGDSAALAPFLGSGTRVIDAKGGLITPGFNDAHTHFVDGGFQLSSVDLRNA
                     ATPQEFVRRIAEFAKTRKPGEWITGGDWDHTLWPGQPLPRREWIDSVTPNTPVFLSRL
                     DGHEALANTAALKAANITKDTPTPTGGEILHDAKTGEPTGIFKDNALALIIRAVPDPS
                     PERVDSAVAHAQAYTESLGLTGTSFVSVPFADWASLRRMEKAGKLTMRFTLYLPLTQW
                     HAVADSVKALGAGDDWVRVAGVKGYMDGSAGARTAKFYEPYSDSAGYTGLFRNPPDSM
                     AKWIGAADSAGLQIAVHAIGDQANGVLLGIYDSVGKAHGARDRRFRVEHAQHLRAQDI
                     PKFGADGIIASVQPSHLTDDGRWIQTRIGPERIKTTYPFRTILDTHGVLAFGSDWDVA
                     TPDPLKGIDAAVTRRTNDGKN"
BASE COUNT         1769 a         3522 c         3512 g         1685 t
ORIGIN      
        1 tcggcgatct cctcggtctc ctgggttcgg tcccagccga ggagatctgc catccggcgc
       61 gccaccgggg gcgcggcggc actcccgtga gccggatcct cgtagaacag atgcgtccgc
      121 cggatcagga catcggacag ccgcatcgcc atttcacgct ccaccgcgtg aaccacctcg
      181 gcccacactt ccgggcggcc gtcgagaatc ggccttccca acgtgcggtc ccgttcgatc
      241 agattcacca gcgccgcgga ctccgacccg taggcatgca cgaggtgccg cgcgaccggc
      301 tccgcgatat cccgcgcccg cgcggattcc accatcactt cgagatccgc gatatcgccg
      361 cccggcagca ccaggtggtc ggtggcggca cgggggggca ccggccgccc gtccgcatca
      421 tggagcttct gcgcgacccg atccaccacg tctcgaccca tcacgcggtg cgtcgtgagc
      481 ttgcctccgg cgatcgtcat cagaccgctt gcgctctcca cgatgcgatg ctcgcgggag
      541 acctgcgccg gcgtcgccga cttctccggc gcgagcaggg gacgcaggcc cgcccaggtc
      601 gagatgacgt cctttggagc gagccgcgct tgcggaaaga gggcgttggc cgagcgcagc
      661 aggtagatga cgtcgtcccc gctggcgcgc acctcgtcgg gggacacgct gtcgtcggta
      721 tcggtggtgc cgatgtaact cacgtcatcc cagggaagga cgaacatgac gcggccgtcc
      781 accggagaga gcagggtgat cgcgttggtg tgcccgatac gggaccgcgg tacgaccacg
      841 tggacgccct tggtggggcg cagcagcgcc ggtgcgttcg ggtcgtccat gcggcggagc
      901 ccgtccaccc acggccccgt cgcattcacc acgaccttcc cctgaatcgt cgcggtctgg
      961 ccggtgagct ggtcctgaac ggccacgccc ttcacggtgc catcgggctt cgagagcgcc
     1021 gccacctcga cgtaattcgc gacctgggca ccggcgagca cggcggcgcg gagattggcg
     1081 agcgtcaggc gggcgtcgtc ggtctgcgcg tcccagtaca accccgcgcc cgtcagcccc
     1141 ttttcccgga gccccggttc catccggcgg gccgctttcc gcgagagcca cttgtgcgtg
     1201 tgcacgttcc ggaacgacgc cagcagatca taaagccaga gtgccgcgaa gatcttccac
     1261 cccggcacgc gggaaccacg ataggcgggg aagaggaagg gcagaggccg cacgaggtgc
     1321 ggtgcgatgt tcagcaggac ccggcgttcc cggctggcct cgaacacgag cctgaagtcc
     1381 ccgtgctcca agtaccgcag cccaccgtga atcagccgtg aggagtgaga gctggtgccg
     1441 cctccgaaat cacccttccc gaccaggccg accgaaagtc cccgcagcgc ggcatcacgt
     1501 gcgatggccg ctccggtgat gccgccgccg atcaccagca aatcgaccgg cgcgtcggtc
     1561 attttcgcga gggcggccgc gcggccgcgg tgagagaacg aggtttcggc tcccaacgaa
     1621 acggcctccg cttggcctgc ccggcagcgt ggtggtaggt ttctctggtt gagtgtgacc
     1681 cgagggagaa tgtaatgcct caaggagacc gccgcgtcgc catcgtcgcc ggatgccgaa
     1741 cgccgttcgc caagtcggga acggtgttcc gggacgtgct cgcagtggac ctcgcccgcc
     1801 acgcgggtcg agagctcctg gagcggacca atctcgaccc gcgggaagtc gatgaggtga
     1861 tcttcgggca ggtcgtcgcg tcgcccctgg tgccgaacgt cgcccgcgag gtcagtctcc
     1921 ttccccagct cccgaaagaa atcccggcct ataccgtcaa tcgagcctgc gcctcctcag
     1981 gccaggcggt caccaacgcc gccgatcaga tcgtgcgcgg gcacgccgac gtcattctcg
     2041 ccgggggagt ggaatccctc tccgacgtcc cgatcctgca cacccaccgg atggcccaga
     2101 tcctgaccga gctgtccaag gccaaatcgc tgggtgcccg gctctccacg ctggcgaaga
     2161 tccggccccg ggacctgatc cccgtctctc cggccatttc cgagccctcc actggggaaa
     2221 cgatggggca gtcggccgag aagatggcca aagagaacgg catcacccgg gaggctcagg
     2281 accagtgggc cctccgcagc cacaagctcg cggcggcggg gaccgcggat gggcgcctca
     2341 aggccgagat cgtgccctgg ctgcccgatg gcggtggtgc cccgatcacg gccgacaacg
     2401 gcatcaggac cgacacgacg ctcgagcaga tggccaagct caagccggtc ttcgaccgcc
     2461 ggtacggatc ggtcacggcc gcgaattcgt ccccactcac cgacggtgcc tccgccgtgc
     2521 tcctcatgag cgaagaggcc gcccgggccc tgggttacaa gccactggcg ttcatcaggt
     2581 cgtatgcggt cgccgcggtg gatccggggt ggcagctcct tcaaggaccc ctgtgggcgg
     2641 tgccgaaggc gctcgagcgc gcggggatcc cgtggagctc gctcggcttg ctcgaggtgc
     2701 acgaggcgtt cgcggcgcag gtcctgtcga atttcaaagg ctggagtgag aagggctgga
     2761 acgtgaaccc cgacatcatc aacgtgatgg gcgggtccat cgccatcggc catcctttcg
     2821 gcgccaccgg cggccgggtg ctgacgaccc tcgccaacga aatgaaccgg cgcgacgtcc
     2881 agttcggcct ggtgtcgatc tgcgcccagg gcgggatggc gttcgcgtgc gttctggagc
     2941 gggtctgatg gccggtccgg cgctgacctg ggagatcgcc gacggcatcg cgatcgtcac
     3001 cttcgacacg cccgggtcgt cggtgaacgt catctcggcg ccggtgaagc aggaagtgat
     3061 ccgggcgttc gacaccatca aagccgacga gcgggtgaag gcggtcgcgt tcctctcggg
     3121 caagcgcgac aacttcatcg cgggcgccga catcgaagaa ttcgtgaagt tgcagaccgc
     3181 ttcggaagcc gagcagctct cagccgatgg tcaggcgatg ctcggcaagg tcgcggaatt
     3241 tcccaagccg gtggtcgtcg gcattcacgg cgcctgtctg ggcggcggac tcgagttctc
     3301 gctctgttgc acctaccgca tcgccaccga tcatcccagg acgtcgatcg ggctccctga
     3361 gatcaagctc ggcattctcc ccggcgccgg cggttgccag cggcttccgc ggctcatcgg
     3421 tgttcgcgct gccctcgaca tcatccttgc cggcaagacg gtccccgcca ggaaggcgct
     3481 ccaggtgggc atcatcgacg agatggtgcc gcaatccatc ctcaggcggc agacgctgga
     3541 gatcgcgaag aaactggcag gtggctggcg tccccaccgg cgccgtccgg gaggcctgat
     3601 gggcttcctg ctcgacggca atccgctcgg gcggatgctc gtgttcaact tggcgcgaaa
     3661 gagcgtcgag aaggagacgc acgggaatta cccggcgccc ctcgcggcgc tcgacgccgt
     3721 cgagttcggc ctcaagcacg ggatgaccgc gggactcgcg catgaagcca gagggttcgg
     3781 ccggctcgcc gtgaccgacg tctcgcgcaa gctcgtccag attttcttcg cggattcggc
     3841 gctcaagaag gacttggggc aagagaatcc cccgccaccg gtcgaagtgc ggcgcctggg
     3901 tgtcgtcgga tcgggattca tgggttcagg gatcgccggt acggccgtgg tccaggctgg
     3961 tgtggatgcc cggctgaagg acgcggacct tggacacgtc tccaagggcg tggccgcggc
     4021 gcgcagtgtc atcgaggacc gctacaagag gcgccggatt accagattcg accgcatcag
     4081 actcctcgcg ctcgtgtccg gagatgcggc gtttaccggg tttggtcgcg ccgagctcgt
     4141 gatcgaggcc gtattcgagg atctcaaggt caagcagcag gtactgaagg aagtcgaagc
     4201 cgcgatccag ccctccgccg tcttcgcgac caacacctcg accatcccca tctcggacat
     4261 cgcctcggcc tcgagcagac ctggccaggt gatcggcatg catttcttct cgcccgtccc
     4321 gcggatgccc cttctggaaa tcatccccgg gaaagccacg gcgccgggca ccgtgagcac
     4381 cgccgtggcg ttcgggcggc ggatgggaaa gacgcccgtc gtcgtgaaag acagcccggg
     4441 cttctgggtg aaccggatcc tggcccccta catgtgcgag gcggcgtacg tgctgctggg
     4501 cgggaccggc atcgacgaaa tcgaccgggc gctcgtgcag ttcggcttcc ccgtgggacc
     4561 cctgaccctg ctggacgaag tcggcctcga cgtggccgtg cacgcatccg aagtgctctt
     4621 caaggcgttc ggcgagcgca tgaagccggc cccggtgctg gaggcgttca tcaaagacca
     4681 gcggctgggg agaaaatcga gcaaggggtt ctacaagtac gagaagggca agaaggccgg
     4741 tgtggacgag accgtgtata cgctcgccgg cgttcacccc aacggcggcc ctcgagccgc
     4801 cgagatcgtg cagcgactga cgctgtccat ggtgaacgag gcggcgcggg ccttctccga
     4861 aggcgtcgtc cgctcgcccc gcgacgggga catcgcggcg atctacgggt tcggcttccc
     4921 gcctttccgc ggcggcccgc tccgctatgc cgacgacctc ggtgccgcgc ggatcgtcga
     4981 cgatctcgag cgcctgaccg aacggttggg catgcgattc acgccctgcg acgcgctgaa
     5041 agacctcgcg cggagcggcg gaaaattcta ctcctgatca tctaaaggat ctacacgacg
     5101 atgctgcgcg ccggcctcct ctggctcagc gagcaaccgc aggtattccg tttcgtccgg
     5161 tccagccgcc tggcgcagaa gttcgcgtcc cggttcgtcg cgggagaaac cgtcgatacc
     5221 gccatcgcgg tcctcaagga cctgaacgcc gcgaatctga gcgtgagcct cgacctcctg
     5281 ggggagtccg tcagttcacc cgaggaagcg aagggcgcgc agcacaacta cctcgagctc
     5341 ctggatcgca tccagagtgc cggtgggaac ggcaacgtct cggtgaaact gacgcagatg
     5401 ggcctcgata tcgacgaagc gctctgtgtg gcgaacctcc ggtcgatcat cggacgggcc
     5461 aagcagtacc actcgttcgt gcggctcgac atggaagggt cggcctacac cgagcggacc
     5521 ctgtcgctgt tcaagcgcac cttctatccc gagttcggcc agtcgacggg agtggtgctg
     5581 caatcctatc tccggcgcac cgcgaaggac gtggatgaga tgatcggctt ggcggcacgg
     5641 gtgcgcctct gcaagggtgc gtacaaggag cccgcgacgg tggcatttcc caacaaggcg
     5701 gacgtcgacg cgaactacgt cgcctgcatg gagcagctga tggagcgggg caactatcca
     5761 ggactcgcca cccacgacga gaagatcatc gagcacgcga aggagttcgc gaagaagaag
     5821 ggcatcggtt ccgagcgctt cgagttccag atgctgtacg gcgtgcgccg tgatttgcag
     5881 atgaagctcc gcaaggaagg ctggaatctc cgggtgtacg tgccctacgg cacgcactgg
     5941 tatccctacc tgatgcgccg gctcgcggag cgcccggcga acctgtggtt catcctcggc
     6001 aacgtgatcc gggagtcgct acgacggagt tgagctcccc cgacggcacg ctgggcggat
     6061 accagcgagt gcatcagcgg gcgccggcgt tcggcggcag cgacgggcag gcctacacgg
     6121 tgtcgacctt cgtggacgat gaccccgatc cggcgggccg gtacggggcg gcactgttgt
     6181 tcattcggtg gtccgaagcc ggcgagcgcc cggtcggcca cgtcgagacc gactacctgg
     6241 ccttcggtcc cgatcccgac gccgccctcg ccctggtcct cgacctgtcc ctggaagacg
     6301 tgaaagccca cctcgaccgc ctcctggcga ctccgatggc cgagtgacct caggcgtcgt
     6361 cttggcccgc tccggctcga cgtaccgggt tcacgtcgag ggccgggaga tcgtggccac
     6421 cctgcggggc aagctcaagc acaaggacga cgatcgcgtc gtggtcggcg acgtcgtcga
     6481 gatggacatg gagtccggcg actcgggcgc gatcacgggc atcaaggagc gccggagcgt
     6541 gctccaacga cgcgccgcgg ggcagggcgc gggccggtcc cagcccatcg cggcgaacgt
     6601 cgatcaggtg ctggtcgtcg cctcggctcg cgatcccgag ctcacgccgc gcatgatcga
     6661 ccggtttctg gtgatcgccg aggcgaacca cctgccggcg ttcgtggtcg tgaacaaggc
     6721 ggaactggac cgcgaggccg gtccggcgct cgcggaccgg taccgggccg ccgagtaccc
     6781 cgttctgctc acgtcggtga aggtgcccga gggattgggt gagctcgagg agcgcctgcg
     6841 ggggcgtgag accgtcctgg ccggccagtc cggcgtgggg aaatcgagct tgctgaacgc
     6901 catcgaaccg gggttgaagc tgcggatcgg ggccatcagc gagaagtggc gcacgggcaa
     6961 gcacaccacc accgcggccg agctgatccc gctcaaggcc ggagggtatg tggtggatac
     7021 gccgggaatg cgggaagtcg ggacctgggg gatcgagccg gccgagctcg ggtcgtgctt
     7081 ccccgaattc cgccaattcc tcggacagtg ccggttcgac aattgccggc acttgaacga
     7141 gccatcctgc gccgtgcggc ggggggcgga cgccggcgct ttccccaagg accgcctgct
     7201 gtcgtacgag cggatctacg aggaaatcaa cgttccttcc tggtccagcg gtcggcgtcg
     7261 cgggtcctga ccttcgccag ggtggctgcg aacgcctgat ccaggtcgat gtgctgagag
     7321 ttcgcgagac agatgacgac gaacaggatg tccccgagct ccagagccag gttcccctcc
     7381 ggctcttccg ccttcttcgt cttctgacca aatcgatgat tgatctcgcg ggccaactcg
     7441 cctacttctt ccgtgagccg tgcgaggttg gtcagcggac tgaaatatcc ttctttatat
     7501 tggctgatcc attgatccac tgccgcctgg gcctcgccaa aagacaccgg agcctccatt
     7561 cgtgaccgct gccaaaccgc tggtgatgcc gtttcgggga gagcgctatg cgcgcccgtc
     7621 actggacgcg gttctggctc ctccgtacga cgtcatttct ccggacgcga ggaagacgct
     7681 cgcggcccgc gatccccaca atatcgtgca cctcattctg ccggaagcgc cggtggcgga
     7741 ggagaagtac gcgcaggccg cgcgcctcct ccgcgagtgg cggaaaaccg gcgtgctggc
     7801 cgaggatccg gggccgtcgg tctacgtcgt cgcccaggat tacaccctgc cgtcgggcga
     7861 acatcgcacc cgcgtgggca tgctcgccgc cgtctcggcc gagccctacg agaccaggcg
     7921 catcaggcct cacgagaaga cgcacgcggg ccccaaggcc gatcgcctgg cgctcttgaa
     7981 ggaaacccag accggcctgg agtcgatctt cctcctcgcg cccgacgcgg accggggact
     8041 gacgaaggcc ctgctcgagg tgacgaagac caagcccgtg gcgcagggga cgctcgacgg
     8101 ggtcggccat cggctttggg tggtgaccgg aaaggcgggc gaccagctgg cggagcgggc
     8161 gtccgcgggc gcgctctaca tcgccgacgg gcatcaccgg tacgaaacgg cggtcgcgta
     8221 tgccaaggag aaccccaaag ccgaccgggt catggccttc atcgtttcgg cccacgaccc
     8281 cgggctcgtc gttctgccga cccaccgcgt catctacggt gcgggccgcg atgtgtcgca
     8341 cttgatcgag cagtggaaac tgtggttcga tgtgacgccg ggcggggacg tgaccgacgt
     8401 cgtgtcccac ctcgccaagg cgggtgcctc tcaccccgcc tgtttcgtcg cactgcccaa
     8461 ggacgggcac accctggtgc tgcgggcgaa cgcgccactc gacgacgtgc cgggtctggg
     8521 cacgagtccc gcgctccggt ctctggatgt ggcggtcatc gagccgctcg tcgtgaagca
     8581 gattctcggc gccggaacct ccacgccgac gctcacgtac acgcccgacg ccagggtggc
     8641 gctggacgcc gtgaagaagg gccacgccgc ggcgtccgtg ttgctcaacc cgaccaaggt
     8701 gtcgcaggtg gtcgccgtgg cggacgccgg agaagtgatg ccgcagaagt ccacctactt
     8761 cgtgcccaag gtgcccgccg gcctggtcct caaaccatga gcgcaggccg tccgcgggaa
     8821 aagatcctcg agaatctcga gtccgtgtac cgcgaggcat acgaccgcgc caagacggcg
     8881 ggggatgagc gccgcatggc cgatctggat gccgcatatc agcgggaaca gctcctgctg
     8941 gaagtgctcc tcgatatccg ggacggcctg acgtcgaggt ccgctccagc ggcgcccgca
     9001 ccgtccgggc ccgacccaat cgccgtcgtc gacaccatcc gccgaatcag ccagctccgc
     9061 taagggagat ttgtgatgcg ccgcctgtct ttgctgctcc tcttgcccgt ggccgcgtgc
     9121 ggccagaagg ccgatctcgt catcaaccac ggcatggtct ggaccggctt gacctcgagc
     9181 ggtcctcagc ccggcggcgt ggccatccag ggcgacaaga tcgtcgccgt cggcgacagc
     9241 gcggccctcg ctccgttcct gggctccggc acccgggtga tcgacgcgaa gggcgggctg
     9301 atcacgcccg gattcaacga tgcgcacacg cacttcgtgg acggcgggtt tcagctctcg
     9361 tccgtggatc ttcggaacgc cgcgacgccc caggagttcg tgcgccgcat cgcggagttc
     9421 gcgaagaccc ggaagccggg ggagtggatc accggcggtg actgggacca cacgctctgg
     9481 ccgggccagc ccctgccgcg tcgcgagtgg atcgattccg tcacaccgaa cacgccggtc
     9541 ttcctcagtc gtctcgatgg tcatgaagca ctcgcgaaca cggccgccct gaaagccgcg
     9601 aacatcacga aagacacgcc gactccgacg gggggagaaa ttcttcacga cgcgaagacc
     9661 ggagagccga cggggatctt caaggacaat gccctggcgc tgatcatccg agcggtcccc
     9721 gacccgtcac cggagcgcgt cgactccgcc gtcgcgcacg cgcaggcgta caccgagtcc
     9781 ttgggactca cggggacgtc gttcgtgtcg gtgccctttg cggactgggc gagcctccgg
     9841 cggatggaga aggccggcaa gctgacgatg cggtttacgc tgtacttgcc gctcacccag
     9901 tggcacgcgg tggcggactc ggtgaaggcg ctgggtgctg gagacgactg ggttcgcgtg
     9961 gccggcgtca aaggctacat ggacggctcg gcgggcgcgc gcaccgccaa gttttacgag
    10021 ccgtattccg actccgctgg ctacaccggt ctcttccgga atccacccga cagcatggcg
    10081 aaatggatcg gcgccgcgga ctcagcgggc ctccagatcg cggtgcatgc gatcggcgac
    10141 caggcgaacg gagtcctgct cgggatctac gacagtgtgg ggaaggccca cggagcccgc
    10201 gaccggcgtt tccgggtcga gcacgcccag cacctgcgtg cccaggacat cccgaagttc
    10261 ggcgcggacg ggatcatcgc ttcagttcag ccgtctcacc tgaccgacga cggccgctgg
    10321 atccagacgc gcatcggccc cgagcgcatc aagacgacct atcccttccg caccatcctc
    10381 gatacccatg gggttctcgc gttcgggtcc gactgggatg tcgcgacgcc cgaccccttg
    10441 aagggcatcg acgccgcggt cacgcgccgc accaacgacg gcaagaac
//
LOCUS       DASUBP010000017         8585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_95771, whole genome shotgun sequence.
ACCESSION   DASUBP010000017 DASUBP010000000
VERSION     DASUBP010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 8585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8585
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95771"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1556
                     /locus_tag="VFU75_00660"
     CDS             <1..1556
                     /locus_tag="VFU75_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU5465368.1"
                     /translation="MICPRCAVGDISDQTHECTLCGFSPAGVATLADPFPRELEASAR
                     RDLEHEFQIEGVLGQGPRGVVYLALEREQNRLVALKIIPRTPGMAPEADALLQREASL
                     AASLDHPHIVPIYRAGATRIFLWYSMEYSNGRSLAEAIGATDQSAPDWAQCVRIVHQV
                     ASALDYAHRRGMTHGGIKPGNVLLDSKGWARISDFGVARAFGRPTAVFGADAPAEQRY
                     LAPEQFASRNLTGPSVDQYGLAVLAHDCLRGAPPTPHVLEALQRATSEHPSERFATIL
                     DFVTALEADSMLPPPIRPAGALSVVPAPPRRASPNVSAPLVVIDPEAPSVPPRLPRVS
                     VAAPKHRVRSILLTAILLFGGVFGAAWWWLHATPASSAIDPGTIVLQPTNPSDLTQVP
                     AGSTAFNVPPPNAQLPSSPASQQTAAPVTPRPRPATQRPAPSFRRPAAAAGLADGHLL
                     LNTQPWSEVYLDGVAVGNTPKTDLPVSPGTHHLRFTRDGFRPYETSVVVAPGGSLRLT
                     DIVLSPNSP"
     gene            1553..2626
                     /locus_tag="VFU75_00665"
     CDS             1553..2626
                     /locus_tag="VFU75_00665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465369.1"
                     /translation="MTGRRGLLWAALAAVVVAPSGRGLAAQEQNRTLNSALQAYQNLE
                     YETAAALLRRALAVEAPSADTLPTALRPKALTYLAASDYYRGRKDSAAVVFRRLIVQD
                     PRYRPDPLIFPPEVTAAFETARRAMKVVEVITPSDTSIRLGRDQYPIRLYASSFHEVV
                     VGLERPDGRFIKGIYAGPIGDSLDVSWDGFDSTSTPADGNLVLTVTSRPTPGGEVVRV
                     VRIMLDTRTTHADTLPWPTPSDTLLLPERAPSGPALRSLSSGLLGAAAVLLLPSAVGS
                     GTHPAGARYAVVGALSLAGIVGFVTQRPGVPLPDNVAKNVARRAAFQRQIELAKQDNA
                     RRRTGVLERIRAGVPLVISRENP"
     gene            2623..3270
                     /locus_tag="VFU75_00670"
     CDS             2623..3270
                     /locus_tag="VFU75_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465370.1"
                     /translation="MNARLGLLALLALPVTNLAAQSPSRLHFEVQLDGSELYANPIIG
                     SVSSTLSGMVFGGEGRVALGPVGLDLGYWQGSLSSQSGPQPNEDVVEGKALLELAPVH
                     WFTVAAGPFARAYTTPGGTERWLSWRVQGRVEQPLVPETVQAYAELWFVASSNVNVVQ
                     PFTSGRGGRLGLRLEPSSWPVWLTLGYGIEQIRLGSGSRIDTVDRLTFGVGYSRR"
     gene            complement(3267..3590)
                     /locus_tag="VFU75_00675"
     CDS             complement(3267..3590)
                     /locus_tag="VFU75_00675"
                     /inference="COORDINATES: protein motif:HMM:NF016839.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEU5465371.1"
                     /translation="MSAARTPAASAVPALAAMAGALALVIFAIFAGEYSTGDWLKLRR
                     QLAQERDSVMALQGQVDSLGRAAHDLETNPATQERVAREEFGMIRNGETLYRLVPKSE
                     PARAP"
     gene            complement(3587..4867)
                     /gene="eno"
                     /locus_tag="VFU75_00680"
     CDS             complement(3587..4867)
                     /gene="eno"
                     /locus_tag="VFU75_00680"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEU5465372.1"
                     /translation="MSTIIAVRAREILDSRGNPTIEADVTLASGARGRAAVPSGASTG
                     EREAVELRDGDQRRYGGKGVLEAVKNVNEIIAPRLEGFAADEQIAVDSAMLELDGTPN
                     KGHLGANAILSVSMATARAAADEAGLPLYRYLGGPLAHVLPVPMMNILNGGAHASNTV
                     DCQEFMVVPIGAETFPEGLRMGVEVFHALKKVLSKRGLSTAVGDEGGFAPNLPHDEAA
                     LEAIMSAVEAAGFSAGKDIAIALDVAASELFEDGTYSFKKSGGGKKSAEEMIALYKGW
                     VDRYPIVSIEDGLAESDWAGWAKLTEALSSRVQLVGDDIFCTNPDLLGRGIDENVANA
                     ILIKVNQIGTLTETLQCIELARSNGYGAIISHRSGETEDTFIADLAVGSGVGQIKTGS
                     GSRTDRIAKYNQLLRIAEELGDMAHYPGRDLYGL"
     gene            complement(4881..5612)
                     /locus_tag="VFU75_00685"
     CDS             complement(4881..5612)
                     /locus_tag="VFU75_00685"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU5465373.1"
                     /translation="MIRLLWYYLVLVCSSVVHATGTILAALFRVPFKPGGIYDWGTND
                     WSRQVLRGAGTPVDIQGLERIPPGPVVYASNHSSMFDIWALAASLPGSTRFVAKAELG
                     RIPLVGPAMKRAGHVMINRAVKAKAMESYAEAGEKIKSGFSPIVFPEGTRSRTGEMLP
                     FKTTPFALAISAQVPVVPVYVHDTFRILPKGAWRMRSMPIRVRIGEPVPTAGLTLDDR
                     GKLKDQVEAQVRALKARVDSGASGA"
     gene            complement(5609..6571)
                     /gene="thiL"
                     /locus_tag="VFU75_00690"
     CDS             complement(5609..6571)
                     /gene="thiL"
                     /locus_tag="VFU75_00690"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HEU5465374.1"
                     /translation="MTDLPLGKGAEFDRIRAIAKRLGAAASGLGDDVAVIPTGDTNLV
                     ASIDGSVEGVHFRTDWLTFQEIGGRAAAAALSDLAAEGASVIGVLVTIGSPFAGSREP
                     GVVPSDPVTEIMAGVGHMVSSVGGRVLGGDLVKSDKYFVDICALGEAKKPVTRGGARP
                     GDGVWVTGTYGGPRAALEALLKGEKPSVASRARFAHPVPRIAAGQWLSEHGATAMIDV
                     SDGLAADLGHVAAASKVGIALAGDLVPRMEGVSALDALSSGEEYELAVTLPAGFGAKD
                     VTRFESEIRGKLTRVGDVKEGSEVEVRLGGTLVPTPKGFDHFAQ"
     gene            complement(6568..8139)
                     /locus_tag="VFU75_00695"
     CDS             complement(6568..8139)
                     /locus_tag="VFU75_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465375.1"
                     /translation="MRRILAGALPAAVGLLVSSGFWRPGDRVLISDFSEITAVAASSF
                     KVYAATLNGLIIYDRGAGTFDLPQTRLDGYPGSQVRFAVADPVGNGVWLGTQDGWAHY
                     NQDINQWDSGIIPGGARDGFLDAQDPAGGFYVLGLNTWYVIQRGGLAAIPTNNVPGPG
                     RRNGPQSARALLARAPVVSGMLGRLLTDARLRTYQFTSGSMTQDRPNEMFLGTTGLGL
                     LRVDPMSGQGVPLAFGIPNQRASVVVPGPGGVWTVGSARAGERRALTWVAEDLSKFTS
                     NEDALGRGTSVLLAQRMLYSNDALWLGTASGLYQMPGGGGAGRFYDIGAGLPGPNVLS
                     LAKAPDGIWVGTAQGLAHITADGAVTRMTGYDRAVTALVAQRESLWVGTQDGLALLEP
                     GATVPAVPPELAAVATLRAPVVALANAHDTIVVAFADQLGWRNPADGSWTLLRPRAPV
                     GTIAALAPDAGGVWIAGTSGLAFWDIGHQTFHGVSVPNDVPAAIRDVTVEGEYVWAAT
                     DAGLVRLSRDAALHR"
     gene            complement(8136..>8585)
                     /locus_tag="VFU75_00700"
     CDS             complement(8136..>8585)
                     /locus_tag="VFU75_00700"
                     /inference="COORDINATES: protein motif:HMM:NF013425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEU5465376.1"
                     /translation="PHLGEFRALLGDKLADEAANDRWAAAERAAQQLAGTVLLKGVPT
                     VIAGVRGPTHVVASGNPGLATGGSGDLLAGFIGAFLARGLPPVDAAALGAHALGRAAE
                     QGARDWTARSLRPADVLASLPAVWRAWRDAGTCVPPVLLTLDAPETA"
BASE COUNT         1305 a         3146 c         2809 g         1325 t
ORIGIN      
        1 ccatgatctg cccccgctgc gcggtcggtg acatctccga ccagacccat gaatgcaccc
       61 tctgcgggtt ctctcccgcc ggggtggcga cgctcgcgga tccgttcccc cgcgagctcg
      121 aggcgagcgc gcgccgggac ctggagcacg agttccagat cgagggggtg ctgggtcagg
      181 ggccgcgcgg ggtcgtgtat ctcgcgctcg agcgcgagca gaaccggctc gtcgcgctca
      241 agatcattcc gcgcacgccg ggcatggcgc ccgaggcgga cgccctgctg cagcgcgagg
      301 cgtcgctggc agcgtcgctc gaccatccgc acatcgttcc gatctaccgc gccggcgcca
      361 ccaggatctt cctttggtac tccatggagt actcgaacgg ccgttctctg gccgaggcca
      421 tcggtgcgac cgatcaaagc gcgcccgact gggcgcaatg cgtgcgcatc gtccaccagg
      481 tggccagtgc gctcgactat gcccatcgcc ggggcatgac gcacgggggc atcaagccgg
      541 gcaacgtcct gctcgattcc aagggctggg cgcgcatctc ggacttcggt gtcgcccgcg
      601 ccttcggccg gcccaccgcc gtgttcggcg ccgacgcgcc cgcggagcag cggtacctcg
      661 cgcccgagca gttcgcctcg cgcaacctca ccggtcccag cgtcgatcag tacggactcg
      721 ccgtgctggc ccacgattgt ctgcgcggcg cgccaccgac gccgcacgtc ctcgaggccc
      781 tacagcgggc cacgagcgaa catccgtccg agcgcttcgc gaccatcctc gatttcgtca
      841 cggcactcga ggccgactcc atgttgcccc cgccgatcag gccggcgggc gccctctccg
      901 tcgttcccgc tccgccccgc cgcgcgtcgc cgaacgtgag cgcgccgctg gtcgtgatcg
      961 atccggaagc gccctccgtg cccccgcggc tgccccgggt ttcggtcgcg gcgccgaagc
     1021 accgggtccg gagcatcctg ctcacggcca tcctgctctt cggcggcgtc ttcggagcgg
     1081 cgtggtggtg gctgcacgcg acgccggcgt cgagcgcaat cgatcccggc accatcgtgc
     1141 ttcagccgac gaatccgtcc gatctgactc aggtgccggc aggctcgacc gcgttcaacg
     1201 tgccgccgcc gaacgcccag ctgccctcgt cgccggcgtc acagcagacc gcggctccag
     1261 tgaccccgag accgaggccg gcgacccagc gtccggcgcc aagcttcagg cggccggccg
     1321 cggctgccgg cctggcggac ggtcatctgc tcctcaacac gcagccctgg tcggaggtat
     1381 acctcgacgg cgtcgcggtc gggaatacgc ccaagactga cctgccggtg tccccgggca
     1441 cgcaccatct ccggttcacg cgggacggat tccggcccta cgagacttcg gtcgtcgtag
     1501 cccccggcgg ctcgctccgc ctcaccgaca tcgtactgtc gcccaactcg ccgtgaccgg
     1561 gcggcggggt ctgctgtggg cggccctcgc cgccgtcgtc gtcgcgcctt ccgggcgcgg
     1621 tctcgccgca caagagcaga accgcaccct caacagcgcc ctccaggctt accagaacct
     1681 cgagtatgaa accgcggcag cgctgttacg ccgcgccctg gccgtcgaag cccccagtgc
     1741 cgacaccctg ccgacggcgc tgcgacccaa ggcgttgacg tatctcgcgg cgagcgacta
     1801 ctaccgggga cggaaggatt ccgcggcggt ggtctttcgc cggctcatcg tgcaggatcc
     1861 ccgctaccgt cccgacccgc tgatctttcc acccgaagtc acggcagcct tcgaaacggc
     1921 ccggcgcgcc atgaaggtgg tggaggtgat cacacccagc gacacttcca tccggctggg
     1981 acgcgatcaa tatccgatcc gcctctacgc gagctctttc cacgaagtcg tcgtggggtt
     2041 ggagcgcccg gacgggcgct ttatcaaggg catctacgcc ggcccgatcg gcgattccct
     2101 cgacgtgagc tgggacgggt tcgattccac cagtacgccc gccgatggca acctcgtcct
     2161 caccgtgacc tcccgcccga cgcctggcgg cgaagtggta cgggtggtgc gcatcatgct
     2221 cgacacccgg accacgcacg cggatacgct gccctggccg acgccttccg acaccctgct
     2281 cctgcctgag cgggcgccat caggcccggc gctccgttcc ctctcgagcg ggctcctggg
     2341 agcggcggcc gtgctgctcc tgccctcggc ggtgggttcg gggacgcacc cggccggggc
     2401 gcgatacgcg gtcgtcgggg cgttgtccct ggcgggcatc gtcggcttcg tcacccagcg
     2461 gcccggcgtg ccccttcccg acaacgtggc gaagaacgtg gcccggcgtg cggccttcca
     2521 gcggcagatc gagctggcca agcaggacaa cgcgcggcgc cggaccggcg tgctcgagcg
     2581 gattcgcgcg ggtgtcccgc tcgtgatttc gcgggagaac ccatgaacgc ccgcctcggc
     2641 ctcctcgccc tgctcgcgtt gcccgttacg aacctggcgg cgcagagccc gtcccgcctc
     2701 cacttcgaag tccagctcga tggctcggag ctgtacgcaa atccgatcat cggctcggtc
     2761 tcgagcaccc tgtcaggcat ggtgttcgga ggagagggac gggtcgcgct cgggccggtc
     2821 ggcctcgatc tgggatactg gcaaggcagc ctcagcagcc agagcggccc gcagccgaac
     2881 gaagacgtcg tcgaagggaa agccctgctc gaactcgctc ccgtccactg gttcaccgtc
     2941 gccgccggtc cctttgcgcg ggcctacacg acccccggag gcacggagcg ctggctctcc
     3001 tggcgagtcc agggccgcgt cgaacagccg ctggtgccgg aaacggtgca ggcctatgcg
     3061 gagctgtggt tcgtcgcgag cagcaatgtg aacgtggtgc agccgttcac cagcggacgc
     3121 ggcgggcgac tcggactgcg gctcgagccg tcctcgtggc ccgtgtggct tacgctgggt
     3181 tatggaatcg aacagatcag actgggaagt ggatcgcgga tcgacacggt ggaccgcctg
     3241 accttcggtg tggggtacag caggcgctag ggcgccctcg caggctcgct cttcggcacc
     3301 agccggtaga gcgtctctcc gttccggatc atcccgaact cctcacgcgc cacccgctcc
     3361 tgcgtggccg gattggtctc caggtcgtgc gcggcgcggc ccagcgaatc cacttgcccc
     3421 tgcaacgcca tcacggaatc gcgctcctgt gcgagctggc gccgcagctt caaccagtcc
     3481 cccgtcgagt actcacctgc gaagatcgcg aagatcacca gcgccagtgc gcctgccatc
     3541 gcggcgaggg ccgggacggc tgaggcagcg ggggtgcgcg cggcgctcac aggccgtaga
     3601 ggtcccggcc cgggtaatgg gccatgtccc ccagctcttc ggcgatccgc agcagctggt
     3661 tgtacttggc aatgcggtcg gtccgggagc ccgagcccgt cttgatctgg cccaccccgc
     3721 tccccaccgc gagatccgcg atgaaggtgt cttcggtctc acccgagcgg tgcgagatga
     3781 tcgcgccgta gccgttggat cgcgccagct cgatgcattg gagcgtctcg gtgagcgtcc
     3841 cgatctggtt caccttgatg agaatggcgt tcgcgacatt ctcgtcgatg ccgcggccca
     3901 ggaggtccgg gttggtacag aagatgtcgt cgcccaccag ctggacccgt gacgacagcg
     3961 cctccgtgag cttggcccac cccgcccaat ccgactcggc cagtccgtcc tcgatggaca
     4021 cgatcggata ccggtccacc cagcccttgt agagcgcgat catctcttcg gcggacttct
     4081 ttccgccccc gctcttcttg aacgagtagg tgccgtcctc gaagagctcc gaggccgcca
     4141 cgtcgagcgc gatggcgatg tccttgcccg ccgagaaccc cgcggcttcg acggcggaca
     4201 tgatcgcttc aagggccgcc tcgtcgtgcg gcaaattggg ggcgaaaccc ccctcgtcac
     4261 cgacggcggt cgaaagtccg cgcttggaga gcaccttctt gagggcgtga aatacctcga
     4321 cccccatccg caggccttcg gggaaggtct ccgctccgat cgggacgacc atgaactcct
     4381 ggcagtccac cgtattggac gcgtgcgcac cgccgttcag gatgttcatc atcggtacgg
     4441 gcaggacatg cgcgagtggt ccacccaggt accggtagag cggaagtccc gcctcgtccg
     4501 cggcggcccg tgcggtggcc atgctcaccg agagaatggc gttggcgccg aggtggccct
     4561 tgttgggggt tccgtccagc tcgagcatcg cgctgtcgac ggcgatctgc tcgtcggccg
     4621 cgaatccttc gagacggggc gcgatgatct cgttgacgtt cttgaccgct tccagaacgc
     4681 ccttgccgcc gtagcgccgc tggtcgccgt cccgcagctc caccgcctca cgctcacccg
     4741 tcgaggcgcc gctgggtacc gcggcacggc cgcgggcccc ggaggcgagg gtgacatcgg
     4801 cttcgatggt gggattgccc cgggaatcca ggatttcacg cgcgcggacg gcgatgatcg
     4861 tgctcatagt ggcccgaacc ttaggcgccc gaggcgcccg agtcaacgcg cgccttgagc
     4921 gcacggacct gggcctcgac ctggtctttc agtttgccgc ggtcatccaa cgtgagcccc
     4981 gcggtcggca cgggctcgcc gatgcggacc cggatcggca tggaccgcat tcgccacgcg
     5041 cccttgggga ggatcctgaa ggtatcgtgc acgtagacgg gaacgaccgg tacctgcgcc
     5101 gagatggcga gcgcgaaggg cgtggtcttg aacggcagca tctcgccggt ccggctccgc
     5161 gtgccctccg gaaacacgat cggggagaat ccgctcttga tcttctcccc cgcctccgcg
     5221 tacgactcca tcgccttggc tttgacggcc cggttgatca tgacgtgtcc ggcgcgtttc
     5281 atcgcggggc cgacgagtgg gatgcgcccc agctccgcct tcgccacgaa ccgcgtggag
     5341 cccggcagac tcgccgccag cgcccagatg tcgaacatcg acgaatgatt cgaggcgtag
     5401 accaccgggc ccggcgggat ccgctccagc ccctggatgt cgacgggcgt gccggcgccg
     5461 cgaaggacct ggcgcgacca gtcgttcgtg ccccagtcgt agatgccgcc gggtttgaag
     5521 ggcacccgga aaagcgccgc caggatagta ccggtcgcgt ggaccacgct ggagcagacc
     5581 agcacgaggt agtaccagag gaggcggatc attgggcgaa gtgatcgaat cccttgggag
     5641 tcggaacgag cgtgcccccg agcctgacct cgacctccga gccctccttc acatctccga
     5701 cgcgcgtcag cttcccccgg atctcggact cgaagcgggt gacgtccttc gctccgaacc
     5761 ccgcgggcag cgtcacggcc agctcgtact cctctccgct cgacagggcg tcgagcgcgg
     5821 agacgccctc cattcgggga acgagatcgc cggcgagggc gatgccgacc ttggaggccg
     5881 cggcgacatg tccgagatcc gcggcgagcc catcgctcac gtcgatcatc gccgtggccc
     5941 catgctcgga cagccactgc cccgccgcga tccgcgggac gggatgcgcg aaccgggcgc
     6001 gactggcgac cgacggcttc tcccccttga gaagcgcctc gagcgcggct cggggcccgc
     6061 cgtacgtgcc tgtgacccac acgccgtccc cgggccgggc gccaccccgc gtcaccggct
     6121 tcttcgcctc acccagagcg cagatgtcca cgaaatactt gtcactcttt acgaggtcgc
     6181 cgccaaggac tcttccaccc acactcgaca ccatgtgccc cacgccagcc ataatctccg
     6241 tgacgggatc ggaggggacg actcccggct cccggctccc ggcgaaggga ctcccgatcg
     6301 tcacgaggac cccgatgaca ctcgcgcctt cggccgccag atcggagagc gccgcagccg
     6361 ccgcccgccc cccgatctcc tgaaacgtga gccagtcggt acggaagtgg accccttcga
     6421 cactcccatc gatgctggcg acgaggttcg tgtcgcccgt ggggatgacg gcgacgtcat
     6481 cgccgaggcc ggacgcagcg gccccgaggc gcttcgcgat ggcgcgaatc cggtcgaact
     6541 ccgcgccctt cccgagcgga aggtcggtca ccgatggagc gccgcgtcgc gcgacagacg
     6601 caccaggccg gcatcggtag cggcccagac gtattcacct tcgaccgtca cgtcgcgaat
     6661 ggccgccggg acatcgttcg gcacggagac gccgtggaac gtctggtgcc cgatgtccca
     6721 gaacgcgagg ccactggttc cggcgatcca gaccccaccg gcgtcgggcg cgagcgcggc
     6781 gatcgtaccg accggcgcac ggggccgaag cagcgtccag cttccatccg cgggattgcg
     6841 ccagcccagc tggtcggcga aggcgacgac gatggtgtca tgcgcattgg ccagcgccac
     6901 gaccggcgca cgcagcgtcg ccaccgccgc gagctccggg ggcaccgccg gcaccgtcgc
     6961 cccgggctcg agcagggcga gcccgtcctg cgtaccgacc cacagactct cgcgctgggc
     7021 caccagagcc gtcaccgctc ggtcgtatcc ggtcatccgg gtgaccgcgc cgtccgccgt
     7081 gatgtgggcc agtccttgcg ccgtcccgac ccagattcca tccggggcct tggcgagaga
     7141 tagaacattg ggtcccggaa gtcccgcacc gatgtcgtag aaacgccccg ctcccccacc
     7201 ccccggcatc tgatagagac ccgacgcggt gcccagccac agggcgtcgt tcgaatagag
     7261 catgcgctgc gccagcagga ccgacgtccc ccgccccagg gcgtcctcgt tgctcgtgaa
     7321 cttcgacaga tcctcggcca cccacgtcag ggcacgccgc tccccggcac gcgccgatcc
     7381 caccgtccag acgccaccgg ggccaggcac caccactgac gcccgctgat tgggaatccc
     7441 gaacgccagg ggcaccccct gcccgctcat cggatccacg cgcaggagtc cgagtcccgt
     7501 tgtgccaagg aacatctcgt tcgggcggtc ctgggtcatc gagcccgatg tgaactggta
     7561 tgtccggagc cgggcgtcgg tcaggagccg gcccagcatg ccactcacca cgggtgcacg
     7621 ggcgaggagt gcccgggcag actgcggacc gttccgccgg ccgggtcccg ggacgttgtt
     7681 cgtcgggatg gcggcgagtc ctccgcgctg gatcacatac caggtgttca gccccagaac
     7741 gtagaaacca cccgcgggat cctgcgcgtc gagaaagccg tctcgcgccc cgcccggaat
     7801 gatcccggag tcccactgat tgatgtcctg gttgtagtgc gcccacccat cctgggtccc
     7861 gagccatacc ccgttcccga ccgggtccgc caccgcgaac cgcacctgag aaccgggata
     7921 tccgtccagt ctggtctgcg gcagatcgaa cgtgcccgcg ccgcggtcgt agatgatcag
     7981 tccgttgagg gtggcggcgt agaccttgaa cgacgacgcg gcgacggcgg tgatctccga
     8041 gaaatcactg atcaggacgc gatcccccgg ccgccagaat cccgagctca ccaggagacc
     8101 cacggctgcc ggcagcgcgc cggcgaggat gcgcctcacg ccgtctccgg cgcgtcgaga
     8161 gtcagcagga ccggggggac acaggtcccc gcgtcgcgcc acgcgcgcca gaccgccggg
     8221 agtgaggcca acacgtcggc gggtcgcaac gacctcgcgg tccagtcccg cgccccctgc
     8281 tcggccgcac ggcccagcgc gtgggcgccc agtgccgcgg cgtccaccgg gggcagtccg
     8341 cgcgcgagga acgcgccgat gaaccccgcg agcagatcgc ccgagcctcc cgtggcgagc
     8401 cccggattgc cgctcgccac gacgtgggtt ggtccgcgga cgccggcgat caccgtcggc
     8461 acacccttga ggagcaccgt cccggcgagc tgttgtgcag cccgctccgc ggcggcccat
     8521 cggtcgttcg ctgcctcgtc cgcgagtttg tctccaagga gcgcccggaa ctcaccgaga
     8581 tgggg
//
LOCUS       DASUBP010000018         7156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_790886, whole genome shotgun sequence.
ACCESSION   DASUBP010000018 DASUBP010000000
VERSION     DASUBP010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7156
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_790886"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1245)
                     /locus_tag="VFU75_00705"
     CDS             complement(<1..1245)
                     /locus_tag="VFU75_00705"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEU5465377.1"
                     /translation="MTSRRDFLVRTGQVGGALAIAPRLRWTIPSVSEAATVAGPIPAD
                     LLSGLKWRMLGPFRGGRTDAVSGVPGRPEEFYSGSVNGGLWKTIDGGRVWQPIFDDQP
                     VASIGAIAVAPSSPDTVYVGSGESTLRDSTGFGNGVYKSTDAGNTWTHLGLDNTHHIG
                     RVAVDPKNADIVFVAAIGHFYADSAERGIYRSTDGGKTWKKVLDKGPSVGGVDVAIDP
                     SNPKNVFACLWNTRRPPWFTYAPTNGPGGGLYKSTDGGDTWTQMTKGLPAEGIGRSGV
                     AIAASQPSRVYAVIDCLVPEPGATVQPPRPGGFGRDTTPRQGGFFRSDDGGETWTRTS
                     ADQALWGRGWYFEQVVVDPKDPDAVFVSNVSVSRSLDGGKTWVPVRGSPGGDDYHQPW
                     ISPDDSNTLIVASDQGTVISRNA"
     gene            complement(1254..2273)
                     /locus_tag="VFU75_00710"
     CDS             complement(1254..2273)
                     /locus_tag="VFU75_00710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetamidase/formamidase family protein"
                     /protein_id="HEU5465378.1"
                     /translation="MHSRIPSFAVAFALVLPATALAQHRHYLAATPKTVAWGYYSAGA
                     KPVLTVTSGDTVDVETLLTNVPDRLAAAGVPADQIQQSLRDIVTQVTDKGPGGHILTG
                     PIFVQGAEPGDVLEVKILAIHYAIGYGYNGCGGFLPENCATPRTRIIQLDTVANVAHL
                     DSGIVIPLHPFFGSIGDAPAPDSGRVSSNPPGVHAGNLDNRSLVAGTSLFIPVHAPGA
                     LLEVGDGHAAQGDGEVDQTAIETSLLGRFQLIVHKDMHFTWPRGETPTELMTMGTDRD
                     LTKATQIATQQMIEMLMEVKGLSRMEAYRMASMAADLHIAELVDQNVGVYMTIPKSVL
                     GVTHF"
     gene            complement(2390..4003)
                     /locus_tag="VFU75_00715"
     CDS             complement(2390..4003)
                     /locus_tag="VFU75_00715"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU5465379.1"
                     /translation="MYRRIAAFSLLTVAAVAAPGTRAAAQFGPPATGFVVENPILRRM
                     YALGMDSSHAEALAQVLFDSLGPRLTGSPGMEAAQDWAVNTYKSWGLTDARKEQYGTW
                     RGWRRGITHIDLVAPRVRSLEGTMLAWSPGTPKGKVVRAPVVAIPDVADSAAFVAWLP
                     QAKGKFVMVSFPQPTCRPDTSWEHWASPAVYDSMKARRQAAQQAWNERIAHTGYEYRY
                     GHYSLARRLEAAGAAGVISNYWSQGWGVDKIFDTYVDHTPVVDVSCEDYGLLYRLAQH
                     HQGPVVEIQAQSQARGEVPVYNVVASIKGSELPNEYVMLSAHFDSWDGGSGATDNGTG
                     TVMMMEAMRILSMVYPHPRRTILIGDWSGEEEGLLGSKGFVADHQTIVDSLQALFNQD
                     NGTGRVESMSASGYPAASGNLARYLSQIPSDLTRDIKFSFPGIPGGGGTDHASFVMCG
                     APGFGLGSGQWDYGLYTWHTNRDTYDKISFDDLKQNATLVAMLAYLASEDPQFMSRVK
                     RDVMPTGRNGQPMTWPACTPGARSTPAPQ"
     gene            4036..4935
                     /locus_tag="VFU75_00720"
     CDS             4036..4935
                     /locus_tag="VFU75_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase family
                     protein"
                     /protein_id="HEU5465380.1"
                     /translation="MPELPDIETYLAALAPRVLGRPITALRIRSPFVLRTVEPPASAI
                     PGHTVQTLRRLGKRIVFGLDDDLFLVMHLMIAGRLKWKAPGAALPTRIGLLALDFEHA
                     SLLLTEVSKKQRASLHILRGAGALKQLDPGGIDVLAADVRSFGATLTLENHTLKRALT
                     DPKLFSGIGNAYSDEILFAARLSPLALTSRLSEAEIGRLHKATREILTTWTERLKADA
                     KDGFPEKVTAFREGMAVHGRFRKPCPDCGAPIQRIRYAENEVNYCPKCQTGGKVLADR
                     AMSRLLKDDWPRSLEELEARGMT"
     gene            complement(5030..5776)
                     /locus_tag="VFU75_00725"
     CDS             complement(5030..5776)
                     /locus_tag="VFU75_00725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465381.1"
                     /translation="MRGVRGQGSGISLLLAMATSLAAQGKPAAWVTDSAAPCAGLTVG
                     WWVEAAKLQSIVGTRGTVAPRPDGKGLVLLFATTCPHSTIDRTNTGPVALGAVIVRLE
                     ARKDSTRPAGTRWSSAPFVYGPAAAPVTEMFKLYGFATGTGPVILQADSTGGHRTARF
                     VIETQDGKVEANAALGDSTTRFDLTGYLVSAGPEGVFGGHEWANRRFGTATVQASGTT
                     LLSRLGITGPPDLATYDTGFGWNFTLSHEH"
     gene            complement(5773..6624)
                     /locus_tag="VFU75_00730"
     CDS             complement(5773..6624)
                     /locus_tag="VFU75_00730"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HEU5465382.1"
                     /translation="MTDERKPLDEEIDVYGMTHQGLVRKNNQDHFLLGSLRKQMQVLQ
                     TSLPLAVDVGPPDERVAFLAMVADGVGGGPRGEAASQLALSTITQYVTNSAQCYYAAE
                     RPDESFLENLQEGAKRVHDEVVATAEKDPDLKGMATTLTLWLGVWPRVYILQVGDSRC
                     YLYRRGQLTQISRDQTLAQELLDQGALTKTDAMRSPLANVLSSSIGGGQSAPVVTESR
                     NERGNVWLLCSDGLTKHVPDERIAERLKEMKSAKETVQALVQDALDGGGTDNITVIVG
                     TPFKGDA"
     gene            6698..>7156
                     /locus_tag="VFU75_00735"
     CDS             6698..>7156
                     /locus_tag="VFU75_00735"
                     /inference="COORDINATES: protein motif:HMM:NF025114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU5465383.1"
                     /translation="MPTSSREAVLIVGAGPIGLSCAISATRRGLLPLVIDAGAIVESI
                     VRYPVGMTFFTTPERLEIGGHPLVCAGAKATREEALKYYRGVVRTEKLRVRTGAKLLK
                     AVPSPHGLECTIAGRLGVDTMIAERVVLATGYFDHPNALGVPGEQLPHVSH"
BASE COUNT         1208 a         2480 c         2261 g         1207 t
ORIGIN      
        1 cgcattccgc gagatgacgg tgccctgatc ggatgccacg atcagcgtgt tcgagtcgtc
       61 gggcgagatc cagggctgat ggtagtcatc gccccccggg gagccccgta ccggcaccca
      121 cgtcttgccc ccgtcgagcg accgcgaaac cgagacgttc gatacgaaga cggcgtccgg
      181 atctttcgga tcgaccacga cctgctcgaa gtaccacccg cgcccccaga gcgcctgatc
      241 agccgacgtg cgcgtccacg tttctccgcc atcgtccgac cggaagaacc ctccctgcct
      301 cggcgtcgtg tcgcgcccga acccgcccgg acggggcggc tggaccgtcg cacccggctc
      361 tggcacgagg cagtcgatca ccgcgtagac ccgggatggc tgcgacgccg cgatcgccac
      421 gccggagcgc ccgatcccct cagccggcaa tcccttggtc atctgcgtcc acgtgtcgcc
      481 accgtcggtc gacttgtaga ggccgccacc tggaccgttc gtcggggcat acgtgaacca
      541 gggtggacgc cgcgtattcc agaggcaggc gaagacgttc ttggggttcg acggatcgat
      601 ggcgacgtcg acgccaccca cggacggccc cttgtccagc accttcttcc aggtctttcc
      661 cccgtccgtc gaccggtaga tcccgcgctc cgcgctgtcg gcgtagaagt gcccgatcgc
      721 ggcgacgaag acgatgtcgg cgttcttggg atcgaccgcc acccggccga tgtggtgggt
      781 attgtcgagt cccaggtggg tccaggtatt ccctgcgtcg gtcgacttgt agaccccgtt
      841 gccgaacccg gtcgaatcac gaagggtgga ttccccgctg ccgacgtaaa ccgtgtcggg
      901 cgacgagggg gccacggcga tggcgccgat ggaggcgacc ggctgatcgt cgaagatcgg
      961 ctgccagacc ctgccgccgt cgatcgtctt ccagagccca ccattcaccg atcccgagta
     1021 gaactcctcg gggcgcccgg ggacccccga gaccgcgtcg gtccgcccgc cgcgaaaagg
     1081 gccgagcatg cgccacttga gacccgagag caggtccgca gggattggcc ctgcgaccgt
     1141 cgccgcctcg gacaccgacg ggatggtcca acggagtctg ggggcgatag cgagcgcgcc
     1201 acccacctgc ccggtgcgca cgagaaaatc acggcgagag gtcacatcgg ctcctagaag
     1261 tgagtcacac ccaacacgga cttggggatg gtcatgtaca caccgacgtt ctgatccacc
     1321 agctccgcga tgtgcagatc ggcggccata ctggccatcc ggtacgcctc catgcgcgag
     1381 agtcccttca cctccatcaa catctcgatc atctgttgag tggcgatctg cgtggctttg
     1441 gtgaggtcgc gatcggtccc catcgtcatc agctccgtag gcgtctctcc gcgaggccac
     1501 gtgaagtgca tgtccttgtg gacgatgagc tggaagcgcc ccaggagcga cgtctcgatc
     1561 gccgtctgat ccacttcccc gtcaccctgc gccgcgtggc cgtccccgac ctccagcagt
     1621 gcccccgggg cgtgcacggg gatgaaaagg ctggtcccgg ccacgagcga acggttgtcc
     1681 aggttgccgg catgcacccc gggcggattg ctcgacaccc gtccggaatc gggcgccggt
     1741 gcgtcgccga tcgaaccgaa aaacggatgg aggggaatca cgatccccga gtcgaggtgc
     1801 gccacgttgg cgaccgtgtc gagctggatg atccgggtcc gcggggtcgc gcagttctca
     1861 ggaagaaacc ccccgcaccc gttgtagccg tagccgatgg catagtgaat cgcgaggatc
     1921 ttcacttcca gcacgtcacc cggctcggcg ccttgcacga agatcgggcc ggtgaggatg
     1981 tgccctcccg gccctttgtc cgtcacctgc gtcacgatgt ccctgaggga ctgctggatc
     2041 tgatcggcgg gcacgccggc cgccgcgaga cggtcgggaa cgttcgtcaa cagcgtttcg
     2101 acgtcgacgg tgtcaccaga cgtaacagtg agaacgggct tggcgccggc gctgtagtag
     2161 ccccaggcca cggtcttggg agtagcggcc agatagtgcc gatgttgtgc gagggcggtc
     2221 gccggcagaa ccaatgcgaa cgcgactgcg aacgaaggaa ttctggaatg catggtcacc
     2281 gaaactccgt gccgctgcgg ggagtgtcaa ctgcagggat tcgggattgg ggattcgtgt
     2341 gggctcttcc ccatcccgaa tcccgaatcc ccaatcccca atcgcgccct tactgaggtg
     2401 ccggtgtgct ccgtgccccc ggcgtgcagg ccggccacgt catcggctgc ccattccggc
     2461 cagtcggcat cacatcgcgc ttcacccgcg acatgaactg cggatcctcg gaggcgaggt
     2521 aggccagcat cgccaccagc gtcgcgttct gcttcagatc atcgaaggag atcttgtcgt
     2581 aggtgtcccg gttcgtgtgc caggtgtaga gcccgtaatc ccactgtccc gagcccaacc
     2641 cgaaccccgg cgcgccgcac atgacgaacg acgcatgatc ggtcccgccg ccaccgggga
     2701 tgcccgggaa cgagaacttg atgtcccgcg tcaggtccga cggaatctgc gagagatacc
     2761 gggccaggtt ccccgacgcc gcgggatagc ccgacgcgct catgctctcg acgcgtccgg
     2821 taccattgtc ctgattgaaa agagcctgaa gactgtcgac gattgtctgg tgatcggcga
     2881 cgaagccctt ggagccgagg agcccttcct cctcgccact ccaatcgccg atgaggatgg
     2941 tgcgccgcgg atgcggatag accatcgaga gaatccgcat ggcctccatc atcatcaccg
     3001 tcccggtgcc gttatcggtc gcgccggagc cgccgtccca ggaatcgaag tgcgccgaga
     3061 gcatgacgta ttcgttgggc agctcggagc ccttgatgga cgcgacgacg ttgtagaccg
     3121 gcacttcacc acgcgcctgt gactgcgcct ggatctccac caccggaccc tgatggtgct
     3181 gggcgagacg atacagaagg ccgtagtcct cgcaggacac atcgacgacc ggcgtatgat
     3241 cgacgtaggt gtcgaagatc ttgtccacgc cccaaccttg cgaccagtag ttcgagatca
     3301 cgcccgccgc gcccgcggcc tcgagccgcc gcgccagcga gtagtgaccg taccggtatt
     3361 cgtacccagt gtgggcgatc cgctcattcc aggcctgctg cgccgcctgc cggcgcgcct
     3421 tcatcgagtc atagaccgcc gggctggccc agtgctccca gctcgtgtcg ggacggcagg
     3481 tgggctgagg gaaagacacc atcacgaact tccccttggc ctgcggaagc caggcgacga
     3541 atgccgcgct gtcggcgacg tcgggaatcg cgaccaccgg tgcccgcacc accttcccct
     3601 tcggcgtacc gggactccag gcgagcatcg tgccctcgag cgaccggacc cgcggcgcca
     3661 cgaggtcgat gtgcgtgatg ccgcggcgcc agccgcgcca cgtgccgtac tgctccttcc
     3721 tggcgtccgt gagcccccac gacttgtacg tgttcacggc ccagtcctgt gccgcctcca
     3781 tgcccggaga tccggtaagc cgggggccca atgaatcgaa cagcacctgt gcgagcgcct
     3841 cggcgtggga gctgtccatg cccaacgcat acatgcggcg aagaattgga ttctcaacga
     3901 cgaatcctgt ggcgggtggg ccgaattggg cggcggcccg ggtgccgggc gccgcgaccg
     3961 cagcgaccgt gagcagactg aaggcagcga tccgacggta cacgggttgg tctcctggct
     4021 ttagtattgg aacgaatgcc cgaactcccc gacatcgaaa cgtatcttgc ggcgttggcg
     4081 ccccgcgtgc tcggccgacc catcacggcc ctgcgcatcc gcagcccatt cgtgctgcgc
     4141 accgtcgagc cgccggcctc cgcgattccc ggtcacacgg tgcagacgct gcgccgactc
     4201 ggcaagcgga tcgtcttcgg actcgatgac gatctgtttc tggtgatgca cctgatgatc
     4261 gccgggcggc tgaagtggaa agctccgggc gccgctcttc ctacgcgcat cggcctcctg
     4321 gcgcttgact tcgaacacgc ttcactgctc ctgacggaag taagcaagaa acagcgggcc
     4381 tccctacaca ttctgcgcgg cgccggcgcg ctgaagcagc tcgaccccgg cgggatcgac
     4441 gtcctggccg ccgacgtgcg gtccttcggg gccaccctca ccctcgaaaa tcacaccctg
     4501 aaacgggccc tgaccgaccc caagctcttc tccgggatcg gcaacgccta ttcggacgag
     4561 atcctcttcg ccgcccgtct ctcccctctc gccctgacct ccaggctctc cgaggccgag
     4621 atcgggaggc tccacaaggc cacccgggaa atcctgacca cctggacgga acggctgaag
     4681 gccgacgcga aagacggatt cccggagaag gtgacggcct tccgggaggg gatggcggtg
     4741 catggccgct tccggaagcc ctgccccgac tgtggcgcac cgattcagcg gatccggtac
     4801 gccgaaaacg aggtcaacta ctgtccgaag tgccaaacgg gagggaaggt gctggcggac
     4861 cgtgccatgt cgcgcctcct caaggacgac tggccgcggt cgttggaaga gctggaagca
     4921 cgggggatga cctgatgtcg aacccaggct ggaagcctgg gcccgacagg acatgacgct
     4981 tcaagcgtga tgcgtcatcg ggcccaccct tcagggtggg aacgaaggcc tagtgctcgt
     5041 gggaaagggt gaagttccac ccgaacccgg tgtcgtaggt cgcgaggtcc ggtggtcccg
     5101 tgatgccgag gcgcgagaga agcgtggtgc ccgacgcctg cacggtcgcc gtcccaaatc
     5161 gccgattggc ccactcgtgc cctccgaaca ctccttccgg accggccgac accaggtaac
     5221 ccgtgagatc gaagcgcgtc gtggagtcgc ccagcgccgc gttggcctcg actttcccgt
     5281 cctgggtttc gatcacgaaa cgggccgtgc gatgaccgcc ggtcgagtcg gcctgcagaa
     5341 tcacgggacc ggtaccggtc gcgaatccgt agagcttgaa catctcggtc accggcgccg
     5401 ccgctggtcc gtagacgaag ggcgcggagc tccagcgcgt gcccgccggc ctggtcgaat
     5461 ccttccgggc ctcgaggcga acgatcacgg cgccgagggc aaccggaccg gtgttcgtcc
     5521 tatcaatcgt cgagtgcggg caggtcgtcg cgaagaggag caccaggccc ttgccgtcag
     5581 ggcgtggcgc cacggtgcct cgcgtaccca cgatgctctg cagcttcgcc gcctcgaccc
     5641 accacccgac cgtgagtccc gcgcacggcg cggcggaatc cgtaacccag gcggcgggct
     5701 taccctgcgc ggccaacgaa gtcgccatcg cgaggagcag actgatccct gacccctgac
     5761 ccctgacccc tctcatgcgt cccccttgaa cggtgtcccg acgatcactg tgatgttgtc
     5821 agtcccgccg ccgtcgagcg cgtcctgcac cagcgcctgg acggtttcct tggcggactt
     5881 catctccttc agccgctcgg caatccgctc gtccggcacg tgcttggtga gaccgtcact
     5941 gcagagcagc cagacgttgc cgcgctcatt cctcgattcc gtcaccaccg gcgcactctg
     6001 ccccccgccg atggagctcg acagcacgtt cgcgagcggc gaacgcatgg catccgtctt
     6061 ggtgagagcg ccctggtcga gcagctcctg cgccaaggtc tggtcacggg agatctgcgt
     6121 cagctgaccc cggcggtaga gataacagcg gctgtcgccg acctggagga tgtacacccg
     6181 gggccatacg cccagccaca gcgtcaacgt cgtcgccatc cccttgaggt ccgggtcctt
     6241 ctcggccgtc gcgaccactt catcatgcac gcgcttcgcg ccctcctgca gattctccag
     6301 gaacgactcg tcaggccgct cggccgcgta gtaacactgc gcgctgttgg tgacgtactg
     6361 cgtgatggtc gacagcgcga gctggctggc cgcctctcca cgcggtccgc cgccgacccc
     6421 gtcggccacc atggcgagaa acgccacccg ctcgtcgggg ggacccacgt ccaccgcgag
     6481 cgggaggctg gtctgcagca cctgcatttg cttccgaagc gaacccagca ggaagtggtc
     6541 ctggttgttc ttccgcacca ggccctgatg cgtcatcccg tagacgtcga tctcctcgtc
     6601 caagggcttt cgttcgtcgg tcacctggct ctcgctgagg tggctgcgca atactgctcc
     6661 ccccctgccc ccctcgctag aggagtagat tccggcgatg cccaccagct ctcgcgaagc
     6721 cgttctcatc gtcggcgccg gccccatcgg cttgtcctgt gccatctccg ccacccggcg
     6781 aggcctgctg ccgctggtga tcgacgcggg cgccatcgtc gaatccatcg tacggtaccc
     6841 ggtcgggatg acctttttca ccacgcccga gcgcctggaa atcggcggtc atcccctcgt
     6901 gtgcgcgggc gccaaggcga ctcgcgagga ggcactcaag tactatcggg gggtcgtgcg
     6961 gaccgagaag ctgcgggtcc gaacgggcgc gaagctcctg aaggcggtcc cctcaccgca
     7021 cggcctcgaa tgcaccatcg ccggccgcct gggtgtcgac accatgatcg ccgagcgggt
     7081 cgtgctcgcc acgggatact tcgaccaccc caacgcgctg ggcgtcccgg gggagcagct
     7141 gccgcatgtg tcccac
//
LOCUS       DASUBP010000019        10532 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_518324, whole genome shotgun sequence.
ACCESSION   DASUBP010000019 DASUBP010000000
VERSION     DASUBP010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 10532)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10532)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10532
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_518324"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            153..1193
                     /locus_tag="VFU75_00740"
     CDS             153..1193
                     /locus_tag="VFU75_00740"
                     /inference="COORDINATES: protein motif:HMM:NF012407.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEU5465384.1"
                     /translation="MIKHQVAVIAGDGIGPEVIDAAIPILDKAAARHGFSIAWEHLPY
                     SADHYLATGETLPDHAFKHLQNDVAAILLGAIGDPRVPGNEHARDILLGLRFRLDLFI
                     NFRPVELLNPDLTPLKNGKPINFVIFRENTEGVYLGRGYDKGDEHVAEEVNTGKGVER
                     IIRAAFIWAKTNGKTRVTMSDKSNAVPAHRIWQTTFKQVATEFPGIATEHRFVDALAM
                     EMVREPERFQVIVTNNLYGDILSDLGAGLIGGLGISASANLNPSRAGLFEPVHGSAPP
                     LKGKNVANPVASVLTGALMFEQLGHTEAAKELEGAVKKALAAGARTPDLGGTGTTTQV
                     ANVIAEHLGGQQ"
     gene            1211..2305
                     /locus_tag="VFU75_00745"
     CDS             1211..2305
                     /locus_tag="VFU75_00745"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HEU5465385.1"
                     /translation="MGTRKGAFVLTADGKRDKWKVDGPFFSGWEIYHVKGSPVDPNRI
                     YASQSSSWFGQQVQRSNDGGKTWEPTGKEFRYDGTPGTHQWYDGTPHPWEFKRVWHFE
                     PGIDNPDHVYAGIEDAAIFESKDGGASWTELPGLRDHRTGPQWSPGAGGMCLHTIVID
                     PTNAKRLFVAISAAGAFRSDDGGKSWKTITKGLNSKYIPDPTAEVGHCVHRIALHKSR
                     PDVLFMQKHWDVMRSDNAGDQWHEISGNLPTDFGFVIDVHAHEPETVYVVPITSDQEH
                     FPFEGKLRVYRSRTGGNEWEPLTQGLPQKDCYVNVLRDAMAVDSLDDCGVYFGTTGGQ
                     VYASANGGDTWAPIVRDLPAVLSVEVQTLK"
     gene            2302..2598
                     /locus_tag="VFU75_00750"
     CDS             2302..2598
                     /locus_tag="VFU75_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU5465386.1"
                     /translation="MIRVELPVGLRVLANLDREVTLDVPPPVTPRAILDALEGRYPVL
                     RGAIRDFITDRRRPLVRFYACRQDLSHEPLDTLLPDAVARGEEPFLVIGAIAGG"
     gene            2641..3840
                     /locus_tag="VFU75_00755"
     CDS             2641..3840
                     /locus_tag="VFU75_00755"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEU5465387.1"
                     /translation="MKTQGATKDVVFLSGVRTGYGTFGGSLRDQSAIDLGVVASKGAL
                     ARSGLAATDIGHVVFGNALQTSADAIYCARHVGLKSGLPIETPAVTVNRLCGSGFEAI
                     TQGAQLILLGETDAVLCGGAESMSLAPHVVRGARWGIRLGPAVPFEDLLWEALKDPNC
                     GFSMAETAENLAEKYKLGRKEVDEVALGSQQRAKQAWDSGVFKDEVVPVSIKKKGQMV
                     DYAADEHMRPETTLEVLMNLKPYFKKDGVVTAGNASGICDGAAATIIASEDLAKARNL
                     KPLGRLVAWAVAGVEPKYMGIGPAPAARKALAKAGLKLDQMDLVEVNEAFAPQYLAVE
                     KELGLDRAKTNVHGGAIAIGHPLASTGTRITVHLLHALRRAGKKYGLGSACIGGGQGA
                     AVIVEAF"
     gene            3858..4685
                     /locus_tag="VFU75_00760"
     CDS             3858..4685
                     /locus_tag="VFU75_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HEU5465388.1"
                     /translation="MELLLPILAVAGFYLFSGLRILREYERGVVFVLGRSWGAKGPGL
                     IFLPPMLTRMQKVSLRVMAMDVPPQDVITRDNISIKVNAVLYMRVRDPAKAVIEVENY
                     LYATSQLAQTALRSVLGETEMDELLMKREKINAVLKNIIDQRTESWGIEVSAVEVKDV
                     DLPPEMKRAMARQAEAERERRAKVINAEGELQASEKLAQAARIIGSEPAAIQLRYLQT
                     VTEIASEKNSTTIFPIPIDLFKGLLDAIGTRGAAPALPARTGGETVPALRSEEKARS"
     gene            4687..5511
                     /locus_tag="VFU75_00765"
     CDS             4687..5511
                     /locus_tag="VFU75_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465389.1"
                     /translation="MAGELITREALDRIIQRAAELQTGERDIGDGLTEAEVVKLGGEV
                     GIPARYLQQALLEERTRTAVTEATGTLAWLFGPGQIWVERVVPGSRADVEQSLTRWME
                     DEELLQVKRRFADRMSWEPRVGAFASIQRVFSTGGRGYALARAEEIHAQVTQLEPGFC
                     HVRLGAVVANLRRRRVAGGSAIGGTGALMTGAMMVVGAFGVFALAPLAVLVPVGIAFA
                     RAHGRENERVQVSLEQVLDRLERSEIRADRRLANPSLGPVERIADEIRKTFQGWGT"
     gene            5508..6395
                     /locus_tag="VFU75_00770"
     CDS             5508..6395
                     /locus_tag="VFU75_00770"
                     /inference="COORDINATES: protein motif:HMM:NF005875.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEU5465390.1"
                     /translation="MSEIKTVGVLGCGLMGSGIAQVAATAGYKTIVRDMAPPILDKAK
                     GGIEKSLAKFVEKGKLQATDRDAALARLSFTTQVSDLKPCDIVIEAVTEDLGLKNELW
                     KQLDGLCAPTTIFASNTSSLTIAAMAAATSRADRFVGLHFFNPVPLMQLVEVVKTVTT
                     SDESFMRAFAFAKSLGKEAVSAKDNSGFIVNLLLVPYLLDAIRALEHGVAGTADIDKA
                     MQLGCGYPMGPLTLLDFVGLDTTYKIAEIMFAEYREQRYAPPPLLKRMVLAGMYGKKS
                     GKGFYDHSTTPPTVARLGL"
     gene            6412..6960
                     /locus_tag="VFU75_00775"
     CDS             6412..6960
                     /locus_tag="VFU75_00775"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEU5465391.1"
                     /translation="MIFGYDWPRFHAAVNDLPAALLIFAVVFELGGWLLKRESLRWAS
                     VWTLWAGVLGGWIAVLAGEQAEDVIDHGEGIHELMEIHEKLALITMGIFTLVLVWQLW
                     RRFNPPPMETAILRVLAIVGACFLVATGQKGGQMYFEHAAGVRTETMKGEIENRAEGH
                     HHHPGQPDDDDDHDHADTAHTH"
     gene            6974..7426
                     /locus_tag="VFU75_00780"
     CDS             6974..7426
                     /locus_tag="VFU75_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465392.1"
                     /translation="MAAAAVVACNPASSRPAFLPYPEDVSVLLTGPVPAIASAVGGWM
                     ESQGIHTEWINADDGYVESTWYNTDTHQSTSGTGDLGAVMATFKVRVWVDPDAPGKSK
                     MTVEAVYRPNLDPSRPERDLERSAPPGSGGAVLVQRLIDEMQSKFGGS"
     gene            complement(7423..8235)
                     /locus_tag="VFU75_00785"
     CDS             complement(7423..8235)
                     /locus_tag="VFU75_00785"
                     /inference="COORDINATES: protein motif:HMM:NF014078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF116 domain-containing protein"
                     /protein_id="HEU5465393.1"
                     /translation="MATSAPPAPPRAQLIHIETDRRLGHEWDEWNGLPLPSGGDFSAP
                     ASTFFWFGALTIAVVAALGWGVLFLLAPRVAEVWSPLARILEVGWGGLVGVSWLWFAA
                     LLGSYASGKNLLPEHWLERGPFLRLMNLTSLVSRAFNQRDWVEHAAIDVYNTLAVRRG
                     RTVGRGELLVLIPRCLSKLALDGVLSIAGRYDVPVFVATRGQLARRVIRERRPRAVVA
                     VACERDMMTGLRDVAGKLPVLGLTMRLPNGPCRDAMLDLETMERWIEGWVGS"
     gene            8283..9809
                     /locus_tag="VFU75_00790"
     CDS             8283..9809
                     /locus_tag="VFU75_00790"
                     /inference="COORDINATES: protein motif:HMM:NF014910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5465394.1"
                     /translation="MISTDPALRAAYSEGAGIYRIVPNAVAIPRSTDDLVSLLQQETG
                     RQFVPRGAGSGMPGGNVGTDLVVDCSGFRSLSVDPQRQIARVGAGITWKEVNDAAGQV
                     GLRLPPDPSSGAFATSGGMVSTNAAGPRTVQSGSVRRWVESVDVVNYSGEARTVRRGG
                     GPGPLRPTPGPLRFPKTRKNSSGYALDEFLRSGDELDLLIGSEGTLAFITAVEWRLEP
                     LPKEIAGVALGFGDLDDLGDAVPYLLSLGPSALELLDRTLLSFVQAPGLPPDLACLLL
                     VEFERPSPAAARGVVGDAVRGLEGRTRHVQTAVDRKGLEALWSVRRLASPALARLPET
                     RRSLQVIEDGCVPVDRLGAYVAGVRASAGRLGLEITIFGHAGDGHVHVNALPDLTSSD
                     WRERLTILYQNVTDLLVTLGGTPSGEHGDGRLRAPILPRIFGPETMDLFREVKRSYDP
                     LGKMNPGVIIPAAGWSPISNLKVGPGAAPIPPVIAQKLRQIEREAAWSVPKSSLTSDP
                     "
     gene            9826..>10532
                     /gene="thyX"
                     /locus_tag="VFU75_00795"
     CDS             9826..>10532
                     /gene="thyX"
                     /locus_tag="VFU75_00795"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR02170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0009265 - 2'-deoxyribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEU5465395.1"
                     /translation="MEIFRSPKVTLIARPQFVEPAHLPVKWNGDASDGERLAEFAGRI
                     CYMSQHNPIARTTPEYLENIKKQGHGSVLEHAVYVLLIEGISRSCSHELVRHRAGFGY
                     SQLSQRYVDESHAAFVMPPAIIGDAKLEAEWTNQVEAAQAAYVRAVEGLMERYSWVAD
                     KVHRRKMAREAARSVLPNATEVKIVVSGNARAWRTMLELRTGEGAELEIRRMAVACLA
                     ALSKDAPAFFSDFETYKA"
BASE COUNT         1870 a         3529 c         3469 g         1664 t
ORIGIN      
        1 tgcggcggcg tgcttcccgg cgaccgcaag gtgaacttct gtccccactg cgggcagccg
       61 ccctcagggg agctgcgctg tcccgcgtgc ggcgtcgagg tggacgtcgg gtgggcatac
      121 tgcatcgcct gcgggcgctc cacggggttc gaatgatcaa gcatcaggtc gcggtcatcg
      181 ccggcgacgg catcggtccc gaggtcatcg acgcggcgat tccgattctc gacaaggccg
      241 cggcgcgtca cggattctcc atcgcctggg agcacctgcc ctactcggcc gatcattacc
      301 tggcgaccgg tgagacgctc cccgaccacg ccttcaagca cctccagaac gatgtcgcgg
      361 cgatcttgct cggtgccatc ggagatccca gagtcccggg caacgagcac gcgcgggaca
      421 tcctgctcgg cctccggttc cggctcgatc tcttcatcaa tttccgcccc gtcgagctgc
      481 tcaaccccga cctgacgccc ctgaagaatg gcaagcccat caacttcgtg atcttccgcg
      541 agaacaccga gggcgtctac ctcgggcgcg ggtacgacaa gggcgatgag catgtggcgg
      601 aagaggtgaa taccgggaag ggcgtcgagc ggatcatccg cgccgcgttc atctgggcca
      661 agaccaacgg caagacgcgc gtcacgatgt ccgacaagtc gaacgccgta ccggcgcacc
      721 gcatctggca gaccacgttc aagcaggtcg cgactgaatt tccaggcatt gcgacggaac
      781 accgcttcgt ggacgcgctg gccatggaga tggtgcggga gccggaacgc ttccaggtaa
      841 tcgtcaccaa caatctctat ggcgacattc tctcggatct cggtgccggc ctgatcggcg
      901 gcctcggcat ttcggcatcg gcgaacctga atccgagccg cgccggcctg ttcgagccgg
      961 tgcatggctc cgccccgccc ctgaaaggga agaacgtggc gaatccggtg gcgtcagtgc
     1021 tcaccggagc gctgatgttc gagcagctgg ggcacaccga ggcggcgaag gagctcgagg
     1081 gcgccgtgaa gaaggcactg gcagccgggg cccggacccc cgacctgggc ggcacgggta
     1141 caacgacgca ggtggcgaac gtgatcgccg aacatctggg ggggcagcaa tgagcggtgt
     1201 acgggtcctg gtgggaacca gaaaaggcgc ctttgtcctg accgccgacg gcaagcggga
     1261 caagtggaag gtcgacggtc cgttcttctc gggctgggag atctatcacg tcaaaggctc
     1321 ccccgtcgat ccgaaccgca tctacgcctc gcagtcctcc agctggttcg gtcagcaggt
     1381 ccagcgctcg aacgacggcg gcaagacctg ggagcccacc ggaaaagaat tccgctacga
     1441 cgggaccccc ggcactcacc agtggtacga cggcactccg cacccgtggg aattcaaacg
     1501 ggtctggcac ttcgagcccg gcatcgacaa ccccgaccat gtctacgcag gcattgaaga
     1561 cgccgcgatc ttcgaatcga aggatggcgg cgcgtcgtgg accgagctcc ccggcctgcg
     1621 cgatcaccgc accggtccgc agtggtctcc cggcgcgggc gggatgtgtc tccacaccat
     1681 cgtgatcgat ccgacgaacg ccaaacgact gttcgtcgcg atctcggccg cgggcgcgtt
     1741 tcgctccgac gacggcggga aatcctggaa gaccatcacc aaaggcctca actccaagta
     1801 catcccggac ccgaccgcgg aagtgggtca ctgcgtccac cgcatcgcgc tgcacaagtc
     1861 gcggcccgac gtgctcttca tgcagaagca ctgggacgta atgcggagcg acaacgcggg
     1921 cgaccaatgg cacgagatct ccgggaacct gcccaccgat ttcgggttcg tcatcgacgt
     1981 gcacgcgcac gagccagaga cggtctacgt ggtcccgatc accagcgacc aggagcactt
     2041 cccgtttgaa ggcaagctcc gcgtctaccg gagccggacc ggtgggaacg aatgggagcc
     2101 gctgacccaa ggcctcccgc agaaggactg ctacgtgaac gtgctgcgcg acgcgatggc
     2161 ggtcgactca ctcgacgact gcggcgtgta cttcggtacc accgggggcc aggtgtacgc
     2221 ttccgccaat ggcggtgaca cgtgggcgcc gatcgtccgc gacctgcccg cggtcctttc
     2281 ggtcgaggtt cagacactca aatgatccgg gtggaattgc cggtgggcct gcgcgtcctc
     2341 gcgaacctcg atcgcgaagt cacgctcgac gtgcctccac cggtgacacc ccgcgcaatc
     2401 ctcgacgcgc tggaaggccg ctatccggta ctcaggggcg ccatccggga cttcatcacc
     2461 gatcggcgcc gcccgctggt ccgcttttat gcctgccggc aggacctttc gcacgagccg
     2521 ctggacacgc tcctgcctga cgcggtagcc cgcggagaag agccatttct ggtcatcggc
     2581 gcgatcgccg gggggtagtt atcttccgcg ctcacaccgc ttgacggagt tcctgactca
     2641 atgaagaccc aaggcgcaac gaaggacgtc gtgtttctct ccggcgtgcg caccggatac
     2701 ggcaccttcg gcggctccct cagagaccaa tccgccatcg acctcggcgt cgtggcctcc
     2761 aagggcgcgc tcgctcgaag cggactcgcc gccaccgaca tcggacacgt cgtgttcggc
     2821 aacgccctcc aaacctccgc ggacgccatc tactgcgcgc gccacgtcgg cttgaaatcg
     2881 gggctcccga tcgaaacgcc cgccgtgacg gtcaaccgcc tctgcggctc cggtttcgag
     2941 gccatcactc agggggcgca gctcatcctc ctcggtgaga ccgacgcggt gctttgcggc
     3001 ggcgccgaat ccatgagcct cgccccccac gtggtccgcg gagcccgctg gggcatccgg
     3061 ctcgggccgg cggtgccctt cgaggatctg ctctgggaag ccctgaagga cccgaattgc
     3121 gggttctcca tggctgagac ggccgagaac ctggcggaga aatacaagct cggccgcaag
     3181 gaagtcgatg aagtcgccct cggatcccag caacgcgcca agcaggcctg ggactcgggc
     3241 gtgttcaagg acgaggtcgt gccggtttca atcaagaaga agggccagat ggtggattac
     3301 gccgcggacg agcacatgcg gccggaaacc acgctggaag tgctcatgaa cctgaagccg
     3361 tacttcaaaa aggatggcgt ggtgacggcg gggaacgcgt cgggaatctg cgacggcgcc
     3421 gctgcgacga tcatcgcgag cgaggatctg gccaaagcca gaaatctcaa gcccctcggg
     3481 cgtctggtgg cgtgggccgt ggcgggcgtc gagcccaagt acatgggcat cggaccggca
     3541 ccggcggcac ggaaggcgct cgccaaagcc gggctcaagc tggaccagat ggatctcgtc
     3601 gaggtcaacg aagccttcgc cccgcagtac ctcgcggtgg agaaagagct cggcctcgac
     3661 cgcgccaaga ccaacgtgca tggcggcgcg atcgcgatcg gacacccgct cgcgtccacc
     3721 ggcacgcgga tcacggtgca cctgctccac gccctgcgcc gtgccggcaa gaagtacgga
     3781 ctcggctcgg cgtgcatcgg cggtgggcag ggtgccgcgg tgatcgtcga ggcgttctag
     3841 gcccgaagga ggacgcgatg gaactgttgc tccccatcct ggcggtcgcc gggttctatc
     3901 tcttctcggg gctccgcatc ctcagggagt acgagcgcgg cgtcgtattc gtgctcggcc
     3961 gctcatgggg cgcgaagggg ccggggctca tcttcctgcc gccgatgctc acgcgcatgc
     4021 agaaggtgtc gttgcgggtg atggcgatgg acgtgccgcc ccaggacgtg atcacccgcg
     4081 acaacatttc catcaaggtg aacgcggtcc tgtacatgcg ggtgcgggat ccggccaaag
     4141 cggtgatcga ggtcgagaac tacctctacg ccacgagtca gctggcccag acggctctcc
     4201 gctcggtgct gggagagacc gagatggacg agctcctgat gaagcgggag aagatcaacg
     4261 cggtcctgaa gaacatcatc gatcagcgta ccgagagctg gggcatcgaa gtgtccgcgg
     4321 tcgaagtgaa ggacgtggac ctcccgccgg agatgaaacg ggcgatggcg cggcaggccg
     4381 aggccgagcg ggagcgccgc gccaaggtga tcaacgcgga aggcgagttg caggcctccg
     4441 agaagctggc ccaggcggcc cgcatcatcg ggtctgagcc ggccgcgatt cagcttcgct
     4501 atctccagac ggtcacggaa atcgcctcgg aaaagaactc cacgacgatc ttcccgatcc
     4561 ccatcgacct cttcaaggga ctcctggacg cgatcggtac ccgaggggcg gcccccgccc
     4621 tgccggcccg caccggtggt gagacggtcc cggcgctccg cagcgaagag aaggcccgca
     4681 gctgagatgg ccggtgagct gatcacccgg gaggccctgg atcgcatcat ccagcgggcc
     4741 gccgagctcc agacgggaga gcgggatatc ggagacggcc tgaccgaggc cgaggtggtc
     4801 aagctgggag gcgaggtggg gatccccgcc cgctacctcc aacaggctct actcgaagag
     4861 cgtacccgga cggcggtcac ggaggccacc gggacactcg cctggctgtt cggccccggt
     4921 cagatctggg tcgagcgggt ggtgccggga agccgcgccg atgtcgagca gtccctcacc
     4981 cggtggatgg aagacgagga gctgctgcag gtgaaacgtc gcttcgcgga ccggatgagc
     5041 tgggagcccc gggtcggcgc ctttgcctcg attcagcggg tgttctcaac tgggggccgc
     5101 ggctacgctc tcgcacgagc cgaagagatc cacgcccagg tcacccagct cgagcccggg
     5161 ttctgtcacg tgcggctcgg cgcggtggtc gccaatctcc gccggcggcg tgtggctggg
     5221 ggttcggcga tcggcggcac gggggcgctg atgacaggcg ccatgatggt agtcggtgcc
     5281 ttcggcgtct tcgccctcgc tcccctcgcc gtcctggtgc cggtcgggat cgcattcgcc
     5341 cgggcgcacg gacgcgagaa cgagcgggtg caggtgtcgc tcgagcaggt actcgatcga
     5401 ttggaacgca gtgaaatccg tgccgaccgg cggttggcga acccgagcct gggtccggtc
     5461 gagcgcatcg cggacgaaat ccggaagacc ttccaaggct gggggacatg agcgagatca
     5521 agaccgtcgg agtgctgggg tgcggcctga tggggtccgg catcgcgcag gtcgcggcga
     5581 ccgccggcta caagacgatc gtccgggata tggccccgcc catcctggac aaggccaaag
     5641 ggggcatcga aaagtccctc gccaagttcg tcgagaaggg gaagctccag gccaccgacc
     5701 gtgatgcggc gctcgcgcgc ctctccttca ccacccaggt ttccgacctg aagccctgcg
     5761 acatcgtgat cgaggcggtc acggaagatc tggggctcaa gaacgagctc tggaagcagc
     5821 tcgacggtct gtgcgcaccc accacgatct tcgcgtcgaa cacgtcgagc ctcacgatcg
     5881 cggcaatggc ggccgccacc agccgcgccg accgcttcgt cgggctccac ttcttcaacc
     5941 ccgtgccgct gatgcagctc gtcgaggtgg tgaagaccgt gaccacgtcg gacgagtcgt
     6001 tcatgcgtgc gttcgccttc gcgaagtcgc tcggcaagga ggcggtgtcc gcgaaggaca
     6061 actcggggtt catcgtgaac ctgctcctgg tgccatacct tctggacgca atccgggcac
     6121 tcgagcatgg cgtggcggga acggcggaca tagataaggc gatgcagctg gggtgcggct
     6181 accccatggg gcccctcacc ctgctggatt tcgtgggtct cgacaccacc tacaaaatcg
     6241 ccgagatcat gttcgccgaa taccgggagc agaggtacgc gccgcccccg ctcctcaagc
     6301 ggatggtgct ggccgggatg tacggcaaga agagcggcaa gggcttctac gaccactcca
     6361 ccacaccgcc caccgtggcg cggctcggac tctaatcgga cgggggcata cgtgatcttc
     6421 ggctacgact ggccgcgctt ccacgccgca gtcaatgatc tgccggccgc gctcctcatt
     6481 ttcgcggtcg tcttcgaact ggggggctgg ctcctcaagc gggaatcgct tcgctgggcg
     6541 tcggtctgga ccctctgggc cggcgtcctg ggcggctgga tcgcggtgct cgccggcgaa
     6601 caggccgagg acgtcatcga ccatggcgag ggcattcacg agctcatgga gatccacgag
     6661 aagctcgccc tgatcaccat gggcatcttc accctggtac tcgtctggca gctgtggcgc
     6721 cgcttcaacc ctccgccgat ggagaccgct atcctgcgcg tactcgcgat cgtgggggcc
     6781 tgcttcctgg tcgcgaccgg gcaaaagggc ggccagatgt atttcgagca cgcggcgggc
     6841 gtgaggacgg agacgatgaa gggggagatc gagaatcgcg ccgagggcca tcaccatcac
     6901 cccggccaac cggatgacga cgacgatcac gatcatgccg acacggccca tacgcactag
     6961 gctgactgct ctgttggcgg cggcagccgt cgtcgcctgc aacccggcgt cgtcccggcc
     7021 ggcgttcctg ccctacccgg aagacgtgtc cgtcttgctc acgggaccgg ttccggccat
     7081 cgcatccgcg gtggggggct ggatggagtc gcaggggatc cataccgagt ggatcaacgc
     7141 cgacgacggg tacgtggagt cgacctggta caacacggac acccaccagt ccacttccgg
     7201 gaccggtgac ctcggagccg tcatggccac cttcaaggtt cgggtgtggg tcgaccccga
     7261 cgctcccggc aagagcaaga tgacggtcga agcggtgtac cggccgaacc tcgacccctc
     7321 acgtccggag cgggacctgg aacgcagcgc gcccccgggg agcgggggcg cggtgttggt
     7381 ccagcggctg atcgacgaga tgcaaagcaa gttcggcggg agctagctcc cgacccaccc
     7441 ttcgatccag cgctccatcg tttcgagatc gagcatcgca tcccggcagg gcccgttcgg
     7501 cagccgcatc gtaagtccca gcaccggcag tttacccgcc acgtccctca gccccgtcat
     7561 catgtcccgc tcgcaggcca ccgccaccac cgcacgggga cgtcgttcgc gaatcacccg
     7621 gcgcgccaac tgcccacgcg tcgcaacaaa cacaggcacg tcatagcggc cggcgatgga
     7681 cagcacgcca tccagggcca gcttggagag gcagcgcgga atcaacacga gcagctcgcc
     7741 gcgacccacc gtccgccccc ggcgcacggc cagggtgttg tagacgtcga tcgcggcatg
     7801 ctcgacccag tcccgctggt tgaaggcccg ggaaaccagg gacgtgaggt tcatcagacg
     7861 aaggaaggga ccacgctcga gccagtgctc gggaaggagg ttcttccctg acgcatagga
     7921 gccgaggagc gcggcgaacc agagccagct gacacccacc agcccgcccc agcccacttc
     7981 caggatcctc gccagcgggg accagacctc cgccacccgg ggcgcgagca ggaagagcac
     8041 tccccacccc agcgcggcca ctaccgcgat ggtcagcgcc ccgaaccaga agaaggtcga
     8101 cgccggcgcc gagaaatccc cgccggatgg caggggcaga ccattccact catcccattc
     8161 gtgacccaaa cgacggtcag tctcgatgtg gatgagttgg gcgcggggag gggccggagg
     8221 ggcagacgtg gccatgggcg aagaagcttc acgctggcgc tcagggatgt caagtttata
     8281 gcgtgatatc gaccgatccc gccctccgcg ccgcctactc cgaaggcgcc gggatctacc
     8341 gcatcgtccc gaacgcggtg gccattcccc gatcgacaga tgacctcgta tcgctcctgc
     8401 aacaggagac ggggaggcag tttgtcccgc gcggtgcggg cagcgggatg cccggcggca
     8461 acgtgggaac ggaccttgtc gtcgattgct caggattccg ctccctctcc gtcgatccgc
     8521 agcggcagat cgcccgcgtg ggagccggca tcacctggaa ggaagtgaat gacgcggcag
     8581 gccaggtcgg cttgcgcctc ccccctgacc cgtcgtcggg ggcttttgcc accagcggtg
     8641 ggatggtttc caccaatgcc gctggtcccc ggaccgtgca gtcgggctcg gttcggaggt
     8701 gggtcgaatc agttgacgta gtcaactatt cgggcgaagc gcgtacggtg cgtcgtggcg
     8761 gggggccggg acccctgcgc ccgacccccg ggccattgcg tttccccaag acccggaaga
     8821 actccagcgg ctacgccctc gacgagttcc ttcgatctgg tgacgagctc gatctcctga
     8881 tcggatccga gggcacgctg gccttcatca ccgcggtgga gtggcgcctc gaacccctcc
     8941 ccaaagagat cgccggtgtg gccctcggct tcggcgatct cgacgacctc ggcgacgccg
     9001 tgccctacct actctccctg ggcccgtccg ccctcgagct gctcgaccgg accctgctct
     9061 ccttcgtaca ggcccccggc ctccctcccg atctggcttg cctgctcctg gtcgagttcg
     9121 aacggccaag ccccgccgct gcccgtgggg tggtgggcga cgccgtgcgg ggcctggaag
     9181 ggcgcacccg gcacgtgcag accgccgtgg accggaaggg cctggaggcg ctgtggtccg
     9241 tacgccgcct cgcctccccg gcacttgccc gcctgcccga gacccggcgc tcactgcaag
     9301 taatagaaga cgggtgcgtc cccgtggacc gcctgggagc ctatgtcgcg ggcgtccggg
     9361 catctgccgg gcgcctcggc ctcgagatca cgatcttcgg gcacgccggg gatggtcacg
     9421 tccacgtaaa cgccctcccc gaccttacca gctccgattg gcgcgagcgc ctcacgatcc
     9481 tctaccagaa cgtgaccgat ctcctggtca ctctcggcgg cacgccgtcg ggcgagcatg
     9541 gcgacggccg gctgcgcgcc cccatcctgc cgcgcatctt cggaccggag accatggacc
     9601 tgttccggga agtgaagcgg tcctacgatc ctctcgggaa gatgaacccg ggtgttatta
     9661 ttcccgccgc cggctggtct ccgatttcga acctgaaggt cggacctggg gctgcgccga
     9721 tccccccggt gatcgcacag aagctccggc agatcgagcg ggaagcagcc tggagcgtcc
     9781 cgaagtcgtc tctgacctct gacccctgac cctggccacc gcctcatgga aatattccgt
     9841 tctcccaagg tcactctgat cgcccgccca cagttcgtgg agccagcgca cctgcccgtc
     9901 aaatggaacg gagacgcgtc agacggggag cgtttggccg agttcgcggg ccggatctgc
     9961 tacatgagcc agcacaaccc catcgcccgc accactccgg aatacctcga gaacatcaag
    10021 aagcaggggc acgggtcggt cctggagcac gcggtctacg tactgttgat cgagggcatt
    10081 tcccgctcct gctcgcacga gttggtgcgc catcgcgccg gtttcgggta ctcccaactc
    10141 tcccagcgct acgtcgacga gtcccacgcg gccttcgtga tgccccccgc catcatcggc
    10201 gacgccaaac tcgaggccga gtggaccaat caggtcgaag cagcccaagc ggcgtatgtg
    10261 agggccgtcg aggggctgat ggagcgctat tcctgggtcg ccgacaaggt ccatcgccgg
    10321 aaaatggcgc gtgaggccgc gcgcagcgtc cttcccaatg ccaccgaagt gaagatcgtg
    10381 gtgtccggga acgcccgcgc gtggcgaacg atgctcgaat tgcgtaccgg agagggcgcc
    10441 gagctcgaga tccggcgcat ggcggtcgcg tgtctcgccg cgctgtcgaa ggacgcgccc
    10501 gcgttcttct ccgacttcga gacgtacaag gc
//
LOCUS       DASUBP010000020        11618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_545721, whole genome shotgun sequence.
ACCESSION   DASUBP010000020 DASUBP010000000
VERSION     DASUBP010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 11618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11618
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_545721"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..736
                     /locus_tag="VFU75_00800"
     CDS             <1..736
                     /locus_tag="VFU75_00800"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5465396.1"
                     /translation="VEGRRMASFEKKVVLVTGVGRPGQIGHAVAGAFGRAGASLVIAD
                     REPPLVKDRVAELEKGGVPVVGVTGDLTTASAARGAVEAAEQEFNGLDAVVNVAGGFL
                     AAGPLAQITAEQLDRTFAINFRTAFNLCQAAAPALARRGGGAIVNFASVAVLHPAGGM
                     SGYSAAKAAVAGLTRSLAVELAPLRIRVNAVAPESVRTPENVSAMGADVPYVEMDDLV
                     RVVLFLASDDARSVSGEVVPLSSIRR"
     gene            733..1464
                     /locus_tag="VFU75_00805"
     CDS             733..1464
                     /locus_tag="VFU75_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5465397.1"
                     /translation="MRLEGRAALVTGAGRRVGQAIAVGLARAGCDVAVHVHGTADGGT
                     DTVRQIRALDRKAEALAADLGDAHAARDLAGRAAALLGGRLDILINSAAIMVKQAVPD
                     VTPESWDRTLDLNLRAPFFVTQGALPFLRKSRGRVVNLADVAGFEPWPAYVPHNVSKA
                     GVVMLTKGLARALAPDITVNAVAPGAVLLPDEWDEKARAHFAETTPLGRLGSPEDVVQ
                     AVLYLLTADYVTGTVLIVDGGRVIR"
     gene            1461..2213
                     /locus_tag="VFU75_00810"
     CDS             1461..2213
                     /locus_tag="VFU75_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465398.1"
                     /translation="MTAPVAMRDVVIVGGGCYGTFYAGQLARAKEKGKASYRQVMIVD
                     RSADCKAKKEFEPAADRTFVTADWTGFFDDYLGSVQPTPDGQPADYIVPSPHMPHLMF
                     EWILRRARARWPGRSVTVEPVPGEIDTPYDRTAHDRTRYVSFADWICPTHCVEPVTCP
                     AIGAARTWEMPETLHALTARLGEGERPVFGPALFVCKHHVFGVGTFAVNQVLAGDAVV
                     SRAGETGAPAAVLVGTVSSCHGAINLLAVGRA"
     gene            2210..2560
                     /locus_tag="VFU75_00815"
     CDS             2210..2560
                     /locus_tag="VFU75_00815"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HEU5465399.1"
                     /translation="MTPEAREAQLKTAQDLFNRGEYWLAHEALETVWRSISRDRAEER
                     LIWQGLIQACAALLHRARGNRHGVIKVGAAAVEKLAGPQRPEVEFETVAFRAELTRAL
                     AGEGDPPRLEWRTR"
     gene            2557..3522
                     /gene="trxB"
                     /locus_tag="VFU75_00820"
     CDS             2557..3522
                     /gene="trxB"
                     /locus_tag="VFU75_00820"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEU5465400.1"
                     /translation="MTDVETKDFDLIIVGAGPAGLCAAMYAGRGMLNAVTLERGAPGG
                     ELLNTDLIEDYPGFASVKGWELAQQMADHATKFGARIETATVDKIERTGDDRFNVTTI
                     GGPTYRAPAVILTAGGTPVKLGVPGEKEYAGKGVSYCAVCDGAFFKGEVLAVVGGGDA
                     AVEEADYLTRYASKVYIVHRRDSFRASKIVQERALANPKIEVIWNKTVPEIAGGEKGV
                     RHLVLEDVNTHERSMLPVGGVFIFIGFKPNTSLVAEHVKHDSGGYLLTDERMMTSVPG
                     LFAAGDVRAQITRQVTTAVGDATTAAIAVEKFLASWRETHAKVPA"
     gene            3519..4148
                     /locus_tag="VFU75_00825"
     CDS             3519..4148
                     /locus_tag="VFU75_00825"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5465401.1"
                     /translation="MNVVQIPGGQFVENCYLVIDEAANACAVIDPGEQAGLILHKLRA
                     SGATPVGIWLTHAHIDHVLGVPELAAETGAPVYLHPDDRELYDAVPDQASWFGLTAEP
                     LPPPDRELAAGERLTVGGLEFAVRHAPGHSPGHVVFAGHGAVFGGDVLFQGSIGRSDL
                     PGGDFDTLIGSIERELLVLPDSTIVYPGHGPATTIGAERRTNPFLRGVA"
     gene            4172..5146
                     /locus_tag="VFU75_00830"
     CDS             4172..5146
                     /locus_tag="VFU75_00830"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEU5465402.1"
                     /translation="MAPKSVPRRAAARIPAGTAASSIRSATALTSWHIVAPHLLALLL
                     LGLDVFVRAWRIQLSVWCAGGKLPLRNAVELNLYGEVASAVTPNRLGGEPARFVGMTE
                     SGLRVVTALVGLGVEVAAEWPVFLVMAAGLAIYYIPDFEIEATRWLRHHLAADLVVIE
                     LAVLLVLVAIYLIQKVVRGGTIRHRVRRQWRVAWAHVRRAPWWVMAANAGLTAVSLAA
                     RAAILPALAYGMPDAPEFSQMFFGALALLAAPLVVPLPSGGGGIEAAFYGGFAGDFGD
                     RQVGMLLLFRIYSIVILAVAGGYLMIRNLGLKAAKELFSIGYGKRAKP"
     gene            5166..6575
                     /locus_tag="VFU75_00835"
     CDS             5166..6575
                     /locus_tag="VFU75_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HEU5465403.1"
                     /translation="MDTRNEKDPLGELPVPAVALYGVQTERARLNFPVSGLAPLPAFV
                     TAVVWIKKAAALTHKDTGRLEARLADAIVTAADEVLAGKHRDQFVVDPYQAGAGTSHN
                     MNANEVLANRANEILGEKRGTYKPVHPNDHVNMAQSTNDVIPTAIRLGALAQLPGLLN
                     ALDGLAQAFLVKGKAFDQILKSGRTHLQDATPVRLGQEFTAYGHTIERNRRRIAQAAE
                     DLRELGIGGSAVGTGLNVEPAYFEAMIRHLRKITGLELRAGADRFQLMQSMGDAAALS
                     GALRTLAVDLGKVANDIRLLVSGPRTGLAEITLPAVQPGSSIMPGKVNPSIAEMVNMV
                     CFQVIGNDATVAAAAEAGQLELNVMMPVIAHNLFFTMHLLTSAATIFTERCVTGIEAD
                     EAMCAHWLDRSPALVTALAPKIGYAESAKLAKEAIAKNETVRQLVTEKGVLKGAELDA
                     VLDLRAMTEPGVPGERKKS"
     gene            6607..7023
                     /locus_tag="VFU75_00840"
     CDS             6607..7023
                     /locus_tag="VFU75_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465404.1"
                     /translation="MSWIVAGLIGGVVAWIADYVLWSKVFTKGMENLALELSPEEMKK
                     FMGPAMAKSAVLALLFGVLFAYLYQRFRSDLWVTGGGILAGMEFATVLWLPTIALQTV
                     GSGVWYVKARGLLSATFWAWLIRMNVVGVVVALLLK"
     gene            7115..8137
                     /locus_tag="VFU75_00845"
     CDS             7115..8137
                     /locus_tag="VFU75_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465405.1"
                     /translation="MNTRFVVFALAGLGLTAPLAAQRPVRIGPIYSSISLDDANGTSH
                     HFTAFGGTIALLTADDDETGISIVRYNDLATSSCPRNLTFYGLDDDYFPVGASGIAPF
                     ASTEVGVARVLDTDKGLLGLSCSAPQTTTEIGFGFGMGLRVTAGKDASGQIEGRFFQV
                     PNSAIQSLELRANVSLLLGPPRVTQMTSGTLGPAVAFLIPISGPLEGRGPLLGVRFRR
                     DTKKASSVLGLEVDWAPLKLTGNCPSTGCSEDAVLFQPGYEASYRPSWGRIYGELGPV
                     LAGFFSQGPDRGVAQGAQGGFGVDVDKHGVLYNVSSRLLWFQPGSGGNVFAVQLGVSV
                     GPAIRP"
     gene            8200..8646
                     /locus_tag="VFU75_00850"
     CDS             8200..8646
                     /locus_tag="VFU75_00850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEU5465406.1"
                     /translation="MTTWAEYGLIVSLSLARRVGDGPVAAREVAEGERLPHDYVEQIL
                     LRLRRAGLVESVRGAKGGYLLARDPHDVSVKDVMEASEHVTFEVNCDHHQVDAHRCSP
                     DAACSIRPVWRMLERRINDFLSGVTLADLTHDEARLYQITGISAAS"
     gene            8660..9808
                     /gene="moeB"
                     /locus_tag="VFU75_00855"
     CDS             8660..9808
                     /gene="moeB"
                     /locus_tag="VFU75_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_257790190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HEU5465407.1"
                     /translation="MTTRTSTRLSHEEVLRYSRHLILPEVGAAGQAKLKSARVLLVGA
                     GGLGSPAALYLAAAGVGTLGVVDADRVDASNLQRQVLHGTSDVGRPKTESAADRLREI
                     NPHVEVERFSERLTSVNALDILRPFDVIVDGSDNFPTRYLVNDACVLLGKPDIYGAIF
                     RFDGQASVFGWHGGPCYRCLFADPPPPDLVPSCAEAGVLGVLPGIIGSIQAAEAIKLI
                     LGIGESLAGRLLLFDALKLEFRELAIARDPECPVCGTRPTVKALIDYEAFCGMPGAGS
                     SATEVTVREFSSERGQGKRHLVVDVREPYEVAIAPFPEAVHISLGQLPGRLAELDAHL
                     PVVTVCHHGQRSMRAREILVAAGFGNVRSLAGGIDAWSREVDPAVPRY"
     gene            complement(9836..9919)
                     /locus_tag="VFU75_00860"
     tRNA            complement(9836..9919)
                     /locus_tag="VFU75_00860"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9883..9885),aa:Leu,seq:cag)
     gene            10016..10471
                     /gene="smpB"
                     /locus_tag="VFU75_00865"
     CDS             10016..10471
                     /gene="smpB"
                     /locus_tag="VFU75_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEU5465408.1"
                     /translation="MDKVAIARNPRARHDFHLLETWEAGLVLTGTEVKSLRDGKASLS
                     EGYAHVKGGEVWLEGLSISPYAAGSYNNPPSLRSRKLLLHKKEIEKLVGGTTRKGLTV
                     VPLELYFKDGIAKVAIALARAKKAHDKREDLKKRDAEREMARVTRGRRA"
     gene            10471..>11618
                     /locus_tag="VFU75_00870"
     CDS             10471..>11618
                     /locus_tag="VFU75_00870"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEU5465409.1"
                     /translation="MFWALVIGLAPTQIVIATPRGQTIVPVTTERGTAAVAAPLLTAP
                     LGLTSVMTDTQTTVTLDGRSFAMQVGVPFARVGTSFCNLAAEPYVARDTLFLPLPFLS
                     ECVPQALPRYRWDAAGGRLIERLTATTAIRPDTDAPVVIHPRPRILPANPMTGLRLHH
                     VVAIDPGHGGVDPGAPKNGGYLPRGQHEKDITFAIARILRSMLLDRGIEVDMTRTTDT
                     LISLYDRPYTCSDACDLFVSIHVNSMPEGRGQSHAHGVETYFLSDATTEDASRVAKME
                     NDAVRYQRDSGADTTHNRFILQDLAQNEILRESAKLAQRIQQQVGAVAPGGDRGVQQA
                     RFWVLSYAHRPAVLVETGFATNRGDAQFLGTGAGQQKIAAAIADGIIA"
BASE COUNT         1826 a         3804 c         4047 g         1941 t
ORIGIN      
        1 cgtcgaggga agacgtatgg cgagcttcga gaagaaggtg gtgctggtca ctggtgtcgg
       61 acgccccggg cagatcgggc atgccgtcgc cggggcgttc ggacgagcgg gcgcgagcct
      121 cgtgatcgcg gaccgcgaac cgccgctcgt gaaggaccgc gtcgcggagc tggagaaggg
      181 cggagtaccg gtggtcggtg tgacgggaga cctcaccacc gcgtcggccg cacggggcgc
      241 ggtggaggcg gcggagcagg agttcaacgg gctcgatgcc gtcgtgaacg tggccggggg
      301 cttcctcgcc gccggacccc tggcgcagat caccgccgag cagctggacc ggaccttcgc
      361 gatcaacttt cgcacggcgt tcaatctctg ccaggctgcc gcaccggcgc ttgcgcgccg
      421 cggcggtggc gccatcgtca acttcgcctc ggtggccgtg ctccatcccg cggggggcat
      481 gagcgggtat tccgccgcga aggccgccgt tgcaggactc acgcggtccc tcgccgtcga
      541 gttggcgcca ctccggatcc gcgtgaatgc cgtcgccccg gagtccgtcc gcacgccgga
      601 gaacgtctcc gcgatgggcg ccgacgttcc ctatgtggag atggatgacc tggtgcgcgt
      661 cgtgctgttc ctcgcgagcg atgacgcgcg gtccgtcagc ggcgaagtcg tcccactgtc
      721 cagcatccgc cggtgaggct cgaaggccgg gccgcgctcg tcaccggcgc cggccgccgg
      781 gtcgggcagg cgatcgccgt gggtctggcc cgcgccggct gcgatgtcgc ggtgcacgtg
      841 cacgggacgg cggatggggg aaccgacacc gtacggcaaa tccgggcgct ggatcggaag
      901 gccgaagcgc tggccgcgga tctcggcgac gcgcacgccg cccgtgacct cgcgggccgc
      961 gccgcggcgc tcctcggcgg acgcctcgac atcctcatca attccgccgc catcatggtg
     1021 aagcaggcgg ttccggacgt cacgccggag tcctgggacc ggacgctgga tctcaacctg
     1081 cgggctccct tcttcgtgac gcagggggcc cttccgttcc tccggaagtc gcgggggcgg
     1141 gtggtgaatc tcgcggacgt cgccgggttc gagccctggc cggcgtatgt cccgcacaac
     1201 gtcagcaaag ccggcgtcgt gatgttgacg aagggtttgg cgcgagcgct cgcacccgac
     1261 atcaccgtga acgccgtcgc gcccggagcc gtgctgctgc ccgacgagtg ggatgagaag
     1321 gcgcgggcgc acttcgcgga gaccacgccg ttgggccggt tggggtcccc cgaggacgtc
     1381 gtccaggccg tgctctatct cctcaccgcc gactacgtaa ccggtacggt gctgatcgtg
     1441 gatggtgggc gggtgatccg atgacggcgc cggtggcgat gcgggatgtc gtgatcgtcg
     1501 gtggcgggtg ctacgggacg ttctacgccg ggcagctggc gcgcgcgaag gagaagggga
     1561 aggcgtcgta ccggcaggtg atgatcgtgg accgcagcgc tgactgcaag gcgaaaaagg
     1621 agttcgagcc ggccgccgat cgtaccttcg tgacggccga ttggacggga ttcttcgatg
     1681 actatctggg ctcggtgcag cccacacccg acggccagcc cgcggactac atcgtgccgt
     1741 cgccgcacat gcctcatctc atgtttgaat ggatcctgcg gcgggcgcgg gcccggtggc
     1801 cgggacgttc cgtgaccgtc gagccggtgc cgggggagat cgatacgccg tacgaccgca
     1861 ccgcgcacga ccggacccgg tacgtctcct ttgccgactg gatttgtccg acgcactgcg
     1921 tcgaacccgt cacctgcccg gccatcgggg cggcgcgcac gtgggagatg ccggagaccc
     1981 tccacgcctt gacggcgcgc ctgggagagg gagaacggcc cgtgttcggt ccggccctct
     2041 tcgtctgcaa gcatcacgtg ttcggcgtcg ggacctttgc cgtcaaccag gtgttggcgg
     2101 gggacgccgt ggtctcgcgg gcgggcgaga cgggagcccc ggccgcggtc ctggtgggga
     2161 ccgtttcgag ctgccacgga gccatcaatc tgctggccgt ggggcgcgcg tgaccccgga
     2221 agcgcgggaa gcccagctca agacggccca ggacctcttc aaccggggcg agtactggct
     2281 ggcgcacgag gcgttggaaa ccgtctggcg ttctataagc agggaccggg ccgaggaacg
     2341 gctgatctgg cagggcctga tccaggcctg tgcggccttg ttgcatcgtg cccgcggcaa
     2401 ccggcatgga gtgatcaaag tgggagcggc ggccgtcgag aagctcgcgg ggccgcagcg
     2461 gccggaggta gagttcgaaa cggtggcgtt ccgcgcggaa ttgacgcgcg ccctggcggg
     2521 ggagggcgat cccccacgtt tggaatggcg gacgagatga ccgacgtgga aaccaaggac
     2581 ttcgatctga tcatcgtcgg ggccgggccc gccgggctct gcgcggcgat gtacgcgggg
     2641 cgcggcatgc tcaacgccgt gacgctcgag cggggcgcac cgggcggcga gctcttgaat
     2701 accgacctga tcgaggacta tccgggcttc gcgagcgtga aggggtggga gctcgcgcag
     2761 cagatggccg atcacgccac gaagttcgga gcccgcatcg agaccgccac ggtggacaag
     2821 atcgagcgga cgggggacga ccggttcaac gtgacgacca tcggtggtcc gacgtaccgg
     2881 gcgcccgccg tcattctcac ggccggaggc acgccggtga agctcggcgt tccgggcgaa
     2941 aaggagtacg cgggcaaggg cgtgtcgtat tgcgcggtct gcgacggcgc ctttttcaaa
     3001 ggtgaggtcc tggcggtggt cggtggggga gacgccgccg ttgaagaggc cgactacctc
     3061 acgcgctacg cgtcgaaggt gtacatcgtg caccgccgcg actcgttccg cgcgtcgaag
     3121 atcgttcagg aacgcgcgct cgcgaatccg aaaatcgaag tgatctggaa caagaccgtt
     3181 ccggagatcg cgggcgggga gaagggcgtg cggcatctcg tgctcgagga cgtgaacacg
     3241 cacgagcgct cgatgctccc ggtgggtggc gtcttcattt tcatcggctt caagcccaat
     3301 accagcctcg tggccgagca tgtgaagcac gactcgggcg ggtacctcct gacggacgag
     3361 cggatgatga cgtcggtgcc cgggctgttc gcggcgggcg acgtgcgggc gcagatcacc
     3421 cggcaggtga cgacggcggt gggggatgcg accaccgcgg cgatcgcggt cgagaaattc
     3481 ctggcgtcct ggcgtgagac gcacgccaag gtccctgcgt gaacgtcgtg cagatcccgg
     3541 gtggacagtt cgtcgagaac tgctacctgg tgatcgacga ggccgcgaac gcctgcgcgg
     3601 tgatcgaccc cggcgagcag gccggtctca tcctccacaa gctccgcgcg tcgggcgcca
     3661 ccccggtggg gatctggctc acccacgcgc acatcgatca cgtgctcggc gtgccggagc
     3721 tggccgccga gaccggtgcg ccggtgtacc tgcacccgga cgaccgcgag ctgtacgatg
     3781 cggtgcccga tcaagcctcc tggttcggct tgaccgccga gccgctccct ccccccgacc
     3841 gggagctggc ggcgggggag cgactcacgg tgggtggctt ggagtttgcc gtccggcatg
     3901 cgccgggcca ttcaccgggg cacgtcgtct ttgcggggca cggggccgta ttcggtggcg
     3961 atgtcctgtt ccagggctcc atcgggcgga gcgacttgcc gggcggagat ttcgacacac
     4021 tgatcggcag catcgagcgg gagttgcttg tactccctga ttccaccatc gtgtacccgg
     4081 gacatggacc cgcaaccact attggcgccg agcgccgaac caaccccttc ctccgtggag
     4141 tcgcctaagc cccggccccg ctgtctccgc tgtggcgccg aagtccgtcc caaggcgcgc
     4201 ggctgcaaga atccctgctg gaactgcggc ttcatctatc cgctcggcga ctgctctgac
     4261 tagctggcac atcgtcgctc cgcatctcct ggccctgctc ctcctggggc tggacgtgtt
     4321 cgtccgcgcc tggcggattc agctctcggt gtggtgcgcg ggcgggaagc ttccgctccg
     4381 caacgccgtc gagctgaacc tgtacggaga ggtggcgtcg gccgtcaccc cgaaccggct
     4441 cggcggggaa cccgcccgct tcgtgggcat gacggagtcg gggctccgcg tcgtgaccgc
     4501 gctggtcgga ctcggcgtcg aagtggcggc cgaatggccg gtgtttctcg tcatggccgc
     4561 cggtctggcc atctactaca tccccgattt tgagattgaa gcgacgcgct ggctgcgtca
     4621 ccacctggcc gcggatctcg tcgtcatcga gctcgcggtg ctgctggtgc tcgtggcgat
     4681 ctacctgatt cagaaagtcg tccgtggggg caccatccgg caccgcgtcc ggcggcaatg
     4741 gcgggtggcg tgggcccacg tgcggcgcgc gccctggtgg gtcatggccg cgaacgcggg
     4801 actcaccgcc gtctcgctcg cggcccgggc cgccatcctt cccgcgctcg cctatgggat
     4861 gcccgacgcg ccggagttca gccagatgtt cttcggcgcg ctcgccctcc tggccgcgcc
     4921 cctcgtcgtc ccgctgccgt cggggggcgg ggggatcgag gccgcgttct acggcggctt
     4981 cgccggcgac ttcggtgacc gtcaggtggg catgctgctg ctgtttcgca tctattcgat
     5041 cgtcatcctg gcggtcgccg gcggctacct gatgatccgg aatctgggat tgaaagcggc
     5101 gaaggagctg ttcagcatcg ggtacggaaa gcgcgccaag ccctagcccg gcctatattc
     5161 ccgccatgga cacgagaaac gaaaaagatc ccctgggcga gctgcccgtt cccgccgtgg
     5221 ccctgtacgg cgtccagacc gagcgcgccc ggctcaactt ccccgtctcc ggactcgcgc
     5281 cgctcccggc gttcgtcacc gcggtggtct ggatcaagaa agccgcggcc ctcacccaca
     5341 aggacacggg gcgcctcgag gccaggctgg cggacgcgat cgtcaccgcg gcggacgaag
     5401 tgctggccgg caagcatcgc gaccagttcg tcgtggaccc ctatcaggcc ggcgccggca
     5461 catcgcacaa catgaacgcc aatgaggtcc tcgcgaatcg cgcgaacgag atcctcggcg
     5521 agaagcgggg gacctacaag ccggtccatc caaacgatca cgtcaacatg gcgcagtcca
     5581 cgaatgacgt gattccgacc gcgatacgcc tcggcgctct cgcgcagctc cccggcctgc
     5641 tcaacgccct cgacggattg gcccaggcgt ttctggtcaa ggggaaggcc ttcgatcaga
     5701 tcctcaagtc ggggcgcacg cacctgcagg atgcgacccc ggtgcgactg gggcaggagt
     5761 tcacggccta tgggcacacc atcgagcgca accggcgccg catcgcccag gccgccgagg
     5821 acctgcggga gctgggaatc ggcggctcgg cggtcgggac gggactgaac gtcgagcccg
     5881 cctacttcga ggcgatgatc cgccatctca ggaagatcac gggactggag ctgcgcgccg
     5941 gcgccgaccg gttccagctg atgcagtcca tgggagacgc cgcggcgctc tcgggcgcgc
     6001 tgcgcaccct ggcggtggac ctcggcaaag tagccaacga catccggctg ctggtgtcgg
     6061 ggccgcggac cggcctcgcg gaaatcaccc tgccggcggt gcagcccgga tcgagcatca
     6121 tgccggggaa agtgaacccg tcgatcgccg agatggtgaa catggtctgc ttccaggtga
     6181 tcggaaacga cgcgacggtc gccgcggcgg cggaagcggg gcagctcgag ctcaacgtga
     6241 tgatgccggt gattgcgcac aacctgttct tcacgatgca cctcctcacc agcgcggcga
     6301 cgatcttcac cgagcgttgc gtgacgggca tcgaggccga cgaggcgatg tgcgcgcact
     6361 ggctggaccg ctcgcccgct ctggtgaccg cgctcgcgcc caagatcggc tacgcggagt
     6421 ccgccaagct ggccaaggaa gcgatcgcca agaacgagac cgtccggcag ctggtgacgg
     6481 agaaaggcgt actgaagggc gccgagctgg acgcggtgct ggatctccgg gcgatgaccg
     6541 agcccggggt gccgggagag cgcaagaaat cctgatcgtc ggaccgttca ctcaaaggag
     6601 ccgtgcatga gctggatcgt ggccgggctg attggggggg tcgtcgcgtg gatcgcggat
     6661 tacgtgctgt ggagcaaggt gttcacgaag gggatggaaa acctcgcgct ggagctctcg
     6721 cctgaggaaa tgaagaagtt catggggccg gcgatggcga agtccgccgt cctcgccttg
     6781 ctgttcggtg ttctgttcgc ttacctctac cagcggttcc gttccgatct ctgggtcacc
     6841 ggtggcggga tcctggcggg catggagttc gccacggtgc tctggctccc gaccatcgct
     6901 ctgcagaccg tcgggtcggg tgtgtggtat gtgaaggcca ggggccttct gtcggcgacc
     6961 ttctgggcct ggctcatccg catgaacgtc gtgggcgtgg tggtggccct gctgctcaag
     7021 tagcgctgtt ttcaatccgc gacaggtttc cccacgtcgg cccggtgaca gctatcacgg
     7081 ctctcaccgg gccgttgtac tgaagtttcc gcacatgaac acgagattcg tggtattcgc
     7141 gctggcgggc ctgggtctga cggccccgct ggcggctcag cgcccggtcc gcatcggccc
     7201 catctactcc tcgatctcgc tcgatgacgc gaacgggacc agtcatcact tcaccgcgtt
     7261 cggggggacc atcgcgttgt tgaccgccga cgacgacgag accgggattt cgatcgtccg
     7321 ctacaacgac ctggcgacgt cgagctgccc gcggaatctg acgttctacg gtctcgacga
     7381 cgactacttc ccggtgggcg cctcggggat cgcgcccttc gcgtcgaccg aggtcggggt
     7441 cgcccgtgtg ctcgacaccg acaagggcct cctcggcctc tcgtgctcgg cgccgcagac
     7501 gacgaccgaa atcggatttg gattcggcat ggggctgcgc gtcaccgcgg ggaaggacgc
     7561 cagcggacag atcgagggac ggttcttcca ggttccgaac agcgccattc agtccctcga
     7621 gcttcgggcc aacgtgtcgc tgctcctggg cccgccgcgc gtcacccaga tgacgagcgg
     7681 cacgctcgga cccgcggtcg cgttcctgat tcccatttcc ggtccgctcg agggccgtgg
     7741 tccactcctc ggggtccgct tccgccgcga caccaagaag gcgtcgagcg tcctcggact
     7801 cgaagtggat tgggcaccgc tgaagctcac cggcaattgt ccgagcaccg ggtgcagcga
     7861 ggacgccgtg ctgttccagc ccggctatga agcgtcgtac cgcccgtcgt ggggacggat
     7921 ctacggagag ctggggccgg tgctcgcggg attcttttcc cagggtcccg atcgcggcgt
     7981 cgcgcagggg gcgcaggggg gcttcggcgt cgacgtggac aagcacggcg tgctgtacaa
     8041 cgtctcgagc cgcctcctct ggttccagcc cggctccggt gggaacgtct tcgccgttca
     8101 gctcggtgtc agcgtggggc ccgcgatccg cccgtaggag cgggccgccg tccgcgagtt
     8161 gataacaccg gcgcgcccgg tcatattccg gccatgcggg tgactacgtg ggcagagtac
     8221 ggattgatcg tctccctcag cttggcgcgc cgggtggggg atggcccggt agcagcccgc
     8281 gaggtcgccg aaggcgagcg tctgccgcac gactacgtcg agcagattct gctccgcctc
     8341 cgccgcgccg gactggtgga gtcggtccgc ggcgccaagg gcggatatct cctcgcccgc
     8401 gacccccacg acgtttccgt gaaggatgtg atggaagcgt ccgagcacgt gaccttcgaa
     8461 gtgaactgcg atcatcatca ggtggacgct caccgttgct cgcccgacgc cgcgtgcagc
     8521 atccggccgg tctggcgcat gctcgagcgg cgcatcaacg acttcctgtc gggggtgacg
     8581 ctcgccgatc tgacccacga cgaggcgcgc ctgtaccaga tcacgggtat ttccgccgcc
     8641 agctgagtat tgcgcctcca tgacaaccag aacctccacc cgcctctcgc atgaagaggt
     8701 cctgcggtat tcccggcatc tcatcctgcc cgaggtcgga gccgcggggc aggcgaagct
     8761 caagagcgcc cgagtcctgc tggtcggagc gggcggtctg ggctctccag cagccctgta
     8821 tctcgccgcg gccggcgtgg gaacgctggg cgtggtcgac gctgatcggg tggatgccag
     8881 caatctccag cggcaggtcc tccacgggac ctcggacgtg ggacgtccca agaccgagtc
     8941 ggccgccgac cggctgcgtg agatcaatcc ccacgtggaa gtcgagaggt tctcggagcg
     9001 gctgaccagc gtgaacgccc tcgatatcct gcgcccgttc gatgtgatcg tggatgggtc
     9061 cgacaacttc cccacacgct atctcgtcaa cgacgcttgt gtgctgctgg gcaagcccga
     9121 catctacggc gcgattttcc ggttcgacgg ccaggcgtcg gtgttcgggt ggcacggcgg
     9181 tccgtgctac cgatgcctct tcgccgatcc gccgcccccc gatctggtgc cgtcgtgcgc
     9241 ggaggcgggc gtgctgggcg tgctgccggg catcatcgga tccattcagg cggccgaggc
     9301 gatcaagctg atcctcggca ttggggaatc cttggcgggg cggctgctcc tgttcgatgc
     9361 gctcaagctc gagttccgtg agctggcgat tgcccgggat cccgagtgtc ccgtgtgcgg
     9421 cacgcggccc acggtcaagg cgctcatcga ctacgaggcg ttctgcggca tgccgggggc
     9481 gggtagtagc gccacggaag tgaccgtgcg cgaattctca tcggagcgcg ggcaggggaa
     9541 gagacatttg gtggtcgatg tccgtgagcc ttatgaggtc gccatcgcgc cgttccccga
     9601 ggctgtccac atctccttgg ggcaactccc gggaaggctc gcggagttgg acgcgcatct
     9661 gccggtcgtg accgtttgcc accatggcca gcggtccatg cgcgcgaggg aaatcctggt
     9721 ggcggccgga ttcgggaacg tgcggagctt ggcgggaggg attgacgcct ggagccggga
     9781 ggtggatcca gcagtaccgc gctactgacc cctgactcct gatacctgct tctcatgccg
     9841 aaggggggac tcgaaccccc acgggcttgc gcccactgcg ccctgaacgc agcgcgtcta
     9901 ccaattccac cacttcggcg tgattcggga acggcaaatc tagacggagg tataacctca
     9961 agtcaacgct tggcttgacc ctcattcacc gcgtacctat tttgcgccga ccgccttgga
    10021 caaagttgcc atcgcccgca atccgagggc gcgacacgac ttccacctcc tcgagacctg
    10081 ggaggccggt ctggtactca ccggaaccga agtgaagtcg cttcgggacg gaaaggccag
    10141 cctctccgaa ggatacgccc atgtgaaggg tggggaagtc tggctggaag gcctcagcat
    10201 ctcgccatac gccgccggtt cgtacaacaa tccgccgtcc ctgcgctccc gcaagcttct
    10261 gctgcacaag aaggaaatcg aaaagctcgt cggcggcacc acccggaagg gtctgaccgt
    10321 cgtgcccctg gagctctact tcaaggacgg catcgccaag gtggcgatcg cgctggcccg
    10381 cgccaagaag gcgcacgaca agcgcgaaga cctcaagaag cgtgacgcgg aacgtgaaat
    10441 ggcccgggtc acgcgggggc gacgagcgtg atgttctggg cgctcgtgat cggtctcgca
    10501 cccacgcaga tcgtgatcgc gacgccgcgg ggccagacca tcgtgccggt gacgaccgag
    10561 cggggtaccg ccgcagtcgc cgcccccctg ctcaccgcac ccctcggcct cacgagtgtg
    10621 atgaccgaca cccagaccac ggtgacgctc gacggccgct cgttcgcgat gcaggtcggc
    10681 gtgccctttg cgcgtgtggg cacgagcttc tgcaatctgg cggccgaacc ctacgtcgcc
    10741 cgggacaccc tcttcctgcc ccttcctttc ttgtcggaat gcgtgccgca ggcactcccc
    10801 aggtaccggt gggatgcggc cggcgggcgc ctgatcgagc gcctcaccgc tacgaccgct
    10861 atccgacccg acaccgatgc gccggtggtg atccacccgc gcccgcggat ccttccggcg
    10921 aacccgatga ccgggttgcg cctgcaccac gtggtggcga tcgatccggg gcatggcggg
    10981 gtggatcctg gtgccccgaa gaacgggggc tacctgcctc gtggacagca cgagaaggac
    11041 atcacgttcg ccatcgccag gatcttgcgg agcatgttgc tggaccgggg tatcgaagtg
    11101 gacatgaccc ggaccaccga tacgctgatc agcctctacg accggcccta cacctgctcc
    11161 gatgcctgcg acctcttcgt cagcatccac gtgaattcga tgcccgaggg gcgggggcag
    11221 tcccacgcgc acggagtgga gacgtacttc ctctcggatg cgacgaccga agacgcgtcc
    11281 cgcgtcgcga agatggagaa cgacgccgtc cgttaccagc gggacagcgg ggcggacacc
    11341 acccacaatc gcttcattct tcaggacctc gcgcagaacg agattctccg ggaatccgcg
    11401 aagctggccc agcgcattca gcagcaggtg ggcgccgtcg ctcccggagg tgaccggggg
    11461 gttcaacagg cgcggttctg ggtcctgagc tacgcgcacc ggccggcggt ccttgtcgaa
    11521 accgggttcg ccaccaaccg cggcgatgcg cagttcctcg gtaccggggc gggccagcag
    11581 aagatcgcgg cggcgattgc ggacgggatc atcgccta
//
LOCUS       DASUBP010000021         5116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_777276, whole genome shotgun sequence.
ACCESSION   DASUBP010000021 DASUBP010000000
VERSION     DASUBP010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5116
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_777276"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..1237)
                     /locus_tag="VFU75_00875"
     CDS             complement(8..1237)
                     /locus_tag="VFU75_00875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5465410.1"
                     /translation="MNRWDAVVVGAGPAGSVTAMLLARRGARVLLLDRARFPRDKPCS
                     EYLTPETTRVLERLGAGILGDVEAKAPARLTGMKLVAPDGTEAVGRFRPGPGYAPPRP
                     YAFALPRTTFDQVLVEAAVGAGVELRESVAVDDLVWDAGTVGGVRAREPRDESRLIRA
                     RVVIGADGLRSVVARRLGFRRSGPPRRIAYSAHVADVSGVTDLGEMHIGLRDRGYVGM
                     GPIGHGETTVALVVPAARAAPELTQPEGFVRALDEFPKIRGRYRADRFTRPILATGPF
                     AQWSRRSVADGVLLVGDAADFFDPFTGQGIYSALTGAELAALVVGEALETETNGPLPA
                     RALEPYRRGRWRAFAGKWVLERLIGFGVGSPSLATRVVRRLARRPDLADLLVGATGNF
                     IPAGRVLHPATLADLIA"
     gene            complement(1234..1812)
                     /locus_tag="VFU75_00880"
     CDS             complement(1234..1812)
                     /locus_tag="VFU75_00880"
                     /inference="COORDINATES: protein motif:HMM:NF014049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HEU5465411.1"
                     /translation="MKWLTTGGVVAAIAVGCATGWGLGWRGVVLLFAFFISGSLLTRL
                     AGGSGGQRTYRQVLANGGVAALAALLGSWPIAAGALAAATADTWATEIGSFSPRAPRL
                     ITTWQPVTKGTDGGVTLLGTAGGISGALFIAGSAALLFAPAMAVVWAGVAGMFVDSAL
                     GATIQGKFLDNDGVNFAATVSGAGTAYLLTHL"
     gene            complement(1809..2033)
                     /gene="rsrA"
                     /locus_tag="VFU75_00885"
     CDS             complement(1809..2033)
                     /gene="rsrA"
                     /locus_tag="VFU75_00885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mycothiol system anti-sigma-R factor"
                     /protein_id="HEU5465412.1"
                     /translation="MKCRECIEHLYEYLDRELTPAVETEIRDHLAHCPPCGTKFDFEA
                     AFKKFVQARTRAQGCPPELKQRILRELFDE"
     gene            complement(2030..2611)
                     /locus_tag="VFU75_00890"
     CDS             complement(2030..2611)
                     /locus_tag="VFU75_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5465413.1"
                     /translation="MATQAPTPADSSRRSAFEKEALVHLDALYRVGLRLTGNAADADD
                     LVQETMLKAYRSWDQYQPGTNAKGWLLTILRNSFINEYRRRTRHPETVDVDTIEPFAV
                     FQDIQEEDPQGAFFDKIVDDEVLRAIDGLPEAFRETVVLSDVEGMSYQEIGKILGIPV
                     GTVKSRLFRARQMLQGKLYDYAVGNGYIKGRKP"
     gene            2673..3278
                     /locus_tag="VFU75_00895"
     CDS             2673..3278
                     /locus_tag="VFU75_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465414.1"
                     /translation="MVHSIVPLAVLEAIRNLDTPVEDGLAEFADELLSKRLGMSATIA
                     MQVNEYEGLVRRDAKVDPANVEALLRLVGRRPDADLVFADAGRRAARRAAARTAGAAK
                     WIARAGPKGLGYAVARRAARVVLNGDLQREHDLPMARMSETLAIRATPDGAACGLYGA
                     AFTELLRLFANFEGSMVHISCRGAGGTACVWRAAAPDPRHH"
     gene            3278..4459
                     /locus_tag="VFU75_00900"
     CDS             3278..4459
                     /locus_tag="VFU75_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HEU5465415.1"
                     /translation="MPPLDGLDLAGVQRALIAMGADGWLLFDFHGLNPIAARVVNVRG
                     LGTRRYFVWLPKTGKPVVIAHKIELTPFTGFPGEVKQYAAWRELHDALTSLLKGKVAA
                     IETSADDNVPYLDRIPHGVVQLLERCGARVVSSAPLVTQFASRWTPDELAGHRRAAEA
                     IAEVAREALQWAGAQVSAGVEVRETTVQRRVREAFDRAGLYTDHGPIVGVQANAADPH
                     YEPQAGSDLLIGKDQVLLLDLWAGPSRGSVFADQTWMGFTGTNPPAEVRKVWETVRGA
                     RDAAVELLRARWASQTPVTGAALDDAARGVIAAAGYGAHFVHRTGHSIDRDLHGSGPH
                     LDNFETSDDRVLMAGIGFSVEPGIYLTGRFGVRSEINVFLGEAGPEVTPRTPQRDLLL
                     V"
     gene            4734..>5116
                     /locus_tag="VFU75_00905"
     CDS             4734..>5116
                     /locus_tag="VFU75_00905"
                     /inference="COORDINATES: protein motif:HMM:NF025092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HEU5465416.1"
                     /translation="MKRFLALGALSAIALVFAAAPSAAQGTTGKVQGTVTNQAGEAVA
                     KAQVIVVGTAFGAVTDDKGYYFINNVPPGTYTFRAQFIGYAPTEVPGVRVLGGQTITQ
                     NFPMKPSAVVVGGINVVAAANPIVPR"
BASE COUNT          795 a         1705 c         1803 g          813 t
ORIGIN      
        1 cccgggttca ggcgatgaga tcggccaggg tggccgggtg aaggacccgg cctgccggaa
       61 taaaattccc cgtggcgccc actagaaggt ccgcgaggtc agggcggcgt gcgagccgtc
      121 tgacgacgcg ggtggcgagg cttggcgagc cgaccccgaa cccgatgagc cgttcgagca
      181 cccattttcc cgcgaacgcg cgccaccgcc cccgccggta cggctcgagg gcacgggcgg
      241 ggaggggtcc attcgtctcg gtctccaggg cttcacccac caccaacgcg gccagttccg
      301 cgcccgtcag cgccgaatag atcccctgtc ccgtgaaggg atcgaagaaa tcggccgcgt
      361 cgccgaccag gaggacgccg tcggcgacgg accgtctcga ccactgagcg aacgggccgg
      421 tcgcgaggat gggacgggtg aaccggtcgg cacggtaccg cccccggatc ttcggaaatt
      481 cgtcgagcgc ccgcacgaat ccctccggct gtgtcagctc cggcgcggcg cgcgcggcgg
      541 ggacgaccag cgccacggtc gtctcgccgt ggccgatcgg tcccatgccg acgtagcctc
      601 gatcacggag gccgatgtgc atctcgccga ggtcggtcac gccggagacg tccgcgacgt
      661 gcgcgctgta cgcgatgcgc cgcgggggcc ccgagcgacg gaatccgagt ctccgagcga
      721 ccaccgagcg cagcccatcc gcaccgatca ccacgcgtgc acggatcaga cggctctcgt
      781 ctctcggctc ccgagccctc acacccccca cggtcccggc gtcccagacc aagtcgtcta
      841 ccgccacgct ctcccgcagc tccactcccg cccccaccgc ggcctcgacg agcacttggt
      901 cgaacgtggt gcgcggcagc gcgaacgcgt aggggcgcgg tggtgcgtac cccgggcccg
      961 ggcggaagcg gccgaccgcc tcggtgccgt cgggcgccac gagcttcatg ccggtgagtc
     1021 gcgcaggggc cttcgcctcg acgtctccga ggatcccggc gccgagtcgc tcgaggacgc
     1081 gagtggtttc gggcgtcagg tattcggagc agggcttgtc gcgcggaaaa cgcgcgcgat
     1141 cgaggagcag tacgcgggcg ccccgtcggg cgaggagcat ggcggtgacc gagccggccg
     1201 gtcccgcgcc gacgacgacg gcgtcccagc ggttcacagg tgggtgagga ggtaagcggt
     1261 accggcgccg ctcacggtgg cggcgaagtt gacgccatcg ttgtccagga acttgccctg
     1321 gatggtagcg cccaacgcgg agtccacgaa cattcccgcg acgccggccc acacgaccgc
     1381 catggcgggc gcaaacaaca gtgccgcgga accggcgatg aagagcgcgc cggagatgcc
     1441 tccggcggta ccgagcaggg tgacgcctcc gtcggtgccc ttcgtgacgg gttgccaggt
     1501 cgtgatgagg cgcggggcgc gcggggaaaa cgagccgatc tccgtggccc aggtgtcggc
     1561 ggtggcggcc gccagggcgc ctgccgcgat gggccaggac cccaggaggg cggcgagggc
     1621 cgcgacgccc ccgttcgcga ggacctgccg gtaggtgcgc tggcccccgc tcccgcccgc
     1681 gagacgggtg aggagggacc cggaaataaa gaaggcgaag agcaggacca ctcctcgcca
     1741 tcccaatccc cagccggtgg cgcagccgac ggcgatcgcc gcgacgacgc cccccgtggt
     1801 caaccacttc attcgtcgaa gagctcccgc aggatgcgct gcttcaattc cggagggcaa
     1861 ccctgggcgc gggtgcgggc ttgaacgaac ttcttgaacg ccgcctcgaa gtcgaacttg
     1921 gtgccgcagg gcgggcagtg cgccagatgg tcgcgaatct ccgtctcgac cgcgggcgtg
     1981 agctcgcgat ccagatactc atagagatgt tcgatgcact cgcggcactt catggcttcc
     2041 gtcccttgat gtacccgttc ccgacggcgt agtcgtacag cttcccctgg agcatctgac
     2101 gggcccggaa cagcctggac ttcacggtgc ccacggggat gccgaggatc ttgccgatct
     2161 cctggtagct catgccttcg acgtccgaca ggaccaccgt ctcccggaag gcttcgggga
     2221 ggccgtcgat ggcccgcagt acctcgtcgt ccacgatctt gtcgaagaag gccccctgcg
     2281 ggtcctcctc ctgtatgtct tgaaacaccg cgaacggctc aattgtgtcc acgtccacgg
     2341 tttccgggtg ccgggtgcgg cgccggtatt cattaatgaa ggaattgcgc aggatggtga
     2401 gcagccaacc cttggcgttg gtgccgggct ggtactggtc ccaggagcgg taggccttga
     2461 gcatggtctc ctgcaccagg tcgtccgcgt ctgcggcgtt cccggtgagg cggaggccca
     2521 cccggtagag cgcgtccaag tgcaccagcg cttctttttc gaatgccgac cggcgggagg
     2581 agtccgcggg ggtgggagcc tgagtcgcca tggggcttta agcctcgccg gacctccccc
     2641 cttccgcaat ggggcactaa gttacgcgcg ccgtggtcca tagcatcgtg ccactcgccg
     2701 ttctcgaggc catccggaac ctggatacgc ccgtggaaga cgggctcgcc gaattcgccg
     2761 acgagctcct gtccaaacgt ctcggaatgt ccgccaccat cgccatgcag gtgaatgagt
     2821 acgagggact ggtgcgccgg gacgccaagg tggaccccgc caacgtcgag gcactgctgc
     2881 ggttggtggg tcgccgtccc gatgccgacc tggtattcgc cgacgccggc cggcgcgcgg
     2941 cgcggcgggc ggcggcgcgg accgcgggcg ccgcgaagtg gatcgcgcgc gccggcccca
     3001 agggcttggg gtatgcggtg gcccgccgcg ccgcgcgcgt ggtcctgaac ggtgatctcc
     3061 agcgggaaca tgatctgccc atggcgcgaa tgagcgagac gctcgcgatt cgtgcgactc
     3121 cggacggcgc cgcgtgcggc ctgtacggcg ccgccttcac cgagctcctc cgattgttcg
     3181 ccaacttcga agggtcgatg gtgcacatca gctgtcgcgg tgccggtggc accgcgtgcg
     3241 tctggcgcgc cgcggcccct gacccccgac accactgatg ccacctctcg acggactcga
     3301 cctcgcgggc gtgcagcgcg ccctcatcgc catgggcgcc gacggctggc tcctcttcga
     3361 ctttcatggc ctgaacccca tcgcggcccg cgtcgtgaac gtccgcggac tcggcacccg
     3421 gcgctatttc gtgtggttgc cgaaaaccgg aaaaccggtg gtgatcgcgc acaagatcga
     3481 gctgacgccg ttcacgggat ttcccggtga ggtgaagcag tacgcggcct ggcgcgagct
     3541 gcacgacgcg ttgacctcgc tgctcaaggg caaggtcgcg gccatcgaga cctccgcgga
     3601 cgacaacgtc ccgtatctcg accggattcc gcatggcgtg gtgcagctgc tcgagcgatg
     3661 tggtgcgcgg gtcgtttcgt ccgcgcccct cgtcacgcag ttcgcctccc gctggacgcc
     3721 ggacgagctg gcggggcacc ggcgggccgc ggaggcgatc gcggaagtgg cgcgggaggc
     3781 gttgcagtgg gcgggcgcgc aggtgtcggc gggagtcgag gtgcgggaga ccacggtgca
     3841 gcggcgggtg cgcgaggcgt tcgaccgcgc ggggctctat accgatcatg ggcccatcgt
     3901 cggggtccag gcgaacgcag ccgatcctca ttacgagccg caggctggca gcgacctcct
     3961 gatcggcaag gatcaggtgc tcctgctcga cctctgggct ggtccgtcac gtgggtcggt
     4021 gttcgcggac cagacctgga tggggttcac cgggaccaat ccgccggcgg aggttcggaa
     4081 ggtctgggag acggttcggg gggcccggga tgcggcggtg gagctgctgc gggcgcgatg
     4141 ggcgtcgcag acgccggtga ccggggcggc actggatgac gcggcacgag gcgtgattgc
     4201 cgcggccgga tacggcgcgc atttcgtgca tcgcacggga cattccatcg accgggacct
     4261 gcacggctcg ggtccgcacc tcgacaattt cgaaacgtcg gatgatcggg tcttgatggc
     4321 cggaatcggc ttctcggtgg agccggggat ctatctgacg gggcggttcg gggtgcgcag
     4381 tgagatcaac gtgttcctcg gggaagccgg gcccgaggtg accccccgga ccccgcagcg
     4441 ggaccttctc ctggtctgac ttaccccttg cggggtgtgg gaccagcctg cattatcgcc
     4501 gccgccgtga tgtcccccgg cggtgacata ggtccgtgac ggggtcgtga cgtctccttg
     4561 acgacctctg tgatggggag cactttaggg ccggtacgat gttcatcgga actgtcagtc
     4621 gaaacctcca cccgaaggga ggtggcgtgt cgtcaccacg ttatccgtcc ggtagtccac
     4681 tcgcattcac gcacttggag gatgattcca tgacgcatcg tgtgagaagc gccttgaagc
     4741 gcttcctcgc actgggtgcg ctttcggcaa ttgcgctggt gttcgcggcg gcgccgtcag
     4801 cggctcaggg cacgacgggg aaggttcaag gcacggtgac gaatcaggcc ggcgaagcgg
     4861 tcgccaaagc acaggtgatc gtcgtcggaa ccgcgttcgg cgccgttact gacgacaagg
     4921 gttactactt catcaacaac gtcccccccg gcacgtacac cttccgcgca cagttcatcg
     4981 gctacgcgcc gaccgaagtg ccgggcgtgc gcgtgctcgg cggtcagacg atcacccaga
     5041 acttcccgat gaagccctcg gccgtcgtgg tcggtggcat caacgtggtg gcggcggcga
     5101 acccgatcgt cccgcg
//
LOCUS       DASUBP010000022         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_572950, whole genome shotgun sequence.
ACCESSION   DASUBP010000022 DASUBP010000000
VERSION     DASUBP010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_572950"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..264
                     /locus_tag="VFU75_00910"
     CDS             <1..264
                     /locus_tag="VFU75_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465417.1"
                     /translation="EDHHTAARACTVCHTDNASPAVRVAHAPPAESHRGCAGCHNPTI
                     VASLVPDRGLCLSCHPMQSAHYPDRPCSGCHAGAGADLRAGKD"
     gene            264..1748
                     /locus_tag="VFU75_00915"
     CDS             264..1748
                     /locus_tag="VFU75_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465418.1"
                     /translation="MRRLFLLLLVPGSLAAQGYRLRLDVRSQVVAYRGVTVDSIPVGD
                     TVSGVGGGPVTPDGIAVQCVSGEPYCSFFRPGPTQRSDPLSTTADFTTWGLGLSGLSV
                     HVLARGGADLGNAAVSWPSTTPAIQLLEGYAEYAQPRYLVRLGRQTQAAPIGFTGFDG
                     ASATVRASRLGLDATGYLGWGLADASALPVTSAALNPLDDFQPVERQLVAGGSLGFRS
                     APLDLHWSYERQIDRRSQYFVSERTRGDAVLRPLPGWTLTASGMYDLAQSLWGNADLR
                     LMWQPRAGFASASVGVSRYRPDFDLWTIWGVFSPTAYRSIDGSLDLTPGHGLSFHVTG
                     QAYKYDPTGASAPLTDFETSGWRMSWDGAYVLSDRWTLDAGYRAEFGPGASSRGFTGG
                     ATFAATNRFTVSAHAGTLDRPLEFRLDDASVWMVGAAATWKASERWNLQADATRYAES
                     RRRPDAAGFDWNQTRLSARLVLFFGSEGRPGLPAAVQAMPEAPR"
     gene            1745..>2036
                     /locus_tag="VFU75_00920"
     CDS             1745..>2036
                     /locus_tag="VFU75_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEU5465419.1"
                     /translation="MRAALLFTAMLLAAGTASGQTPQFDHAKHAKVFPTCVGCHAGAA
                     EVKAALYPDSGVCASCHDGTIQKRVPYVPPDSLRTNLRFSHLGHLAKVPAASC"
BASE COUNT          291 a          717 c          688 g          340 t
ORIGIN      
        1 gaagaccacc acaccgcggc acgggcctgc acggtctgtc acaccgacaa cgcatccccg
       61 gccgtccgcg tggcccacgc gcctcccgcc gaatcccacc gcggatgtgc gggatgccat
      121 aacccgacga tcgtcgcgag cctggtgccc gaccgcgggc tgtgtctgag ctgccatccg
      181 atgcagtccg cccattaccc ggaccggcct tgcagcggct gccacgcggg tgcgggcgcc
      241 gacctgcgcg cggggaagga ctgatgaggc gcctgttcct gctgttactg gttccgggga
      301 gtctcgccgc gcaaggatat cggctgcggc tcgacgtgcg ctcgcaggtc gtcgcgtacc
      361 gaggggtgac cgtcgacagc atccccgtgg gcgacacggt ctcgggggtg ggcggcgggc
      421 ccgtcactcc tgacgggatc gcggtacaat gcgtctccgg cgagccatac tgctcgttct
      481 tccgccccgg cccgacacaa cggagcgacc ccctcagcac gaccgctgat ttcaccacct
      541 ggggcttggg actgtccggg ttgtccgttc acgtgcttgc gcgcggaggc gccgatctcg
      601 gcaatgccgc ggtcagctgg ccgtccacga caccggcgat tcagctgctc gagggatacg
      661 ccgagtacgc ccagccacgg tacctcgtgc gcttggggcg gcagactcag gcggcaccca
      721 tcggctttac gggattcgac ggtgcgagcg ccacggttcg cgcgagccgg ctcgggctcg
      781 acgcgacggg atacctgggc tgggggttgg cggacgcgtc ggctctcccc gtcaccagtg
      841 cggcgctcaa tccgctcgac gacttccaac cggtcgaacg ccaactggtc gccggtgggt
      901 cgctggggtt ccgcagcgcg cccctggatc tgcactggtc atatgaacgg caaatcgacc
      961 gccggtccca gtacttcgtg tcggagcgca cacgcggcga cgccgtgctc cgccccttgc
     1021 cgggatggac gctcacggcg agcgggatgt acgacctggc gcagagcctg tgggggaatg
     1081 ccgacctgcg gctgatgtgg caaccacggg cgggattcgc gagcgcgtcc gtcggcgtga
     1141 gccgctaccg tcctgacttc gacttgtgga ccatctgggg cgtcttcagt cctaccgcgt
     1201 atcggtcaat cgacggatcg ctcgacctca cgccgggaca cggactctct ttccacgtca
     1261 cgggccaggc ctacaagtac gacccgaccg gcgcttccgc acccttgacg gatttcgaga
     1321 cgtcgggctg gcggatgtcg tgggacggcg cctatgtcct gtccgaccga tggaccctgg
     1381 acgccggata ccgcgccgag ttcggtccgg gagcgtcctc tcgaggcttt accgggggcg
     1441 ccaccttcgc cgccacgaac cggttcaccg tgtccgctca cgcgggcacg ctcgatcggc
     1501 cactcgagtt ccggctcgac gacgcatcgg tatggatggt gggtgccgct gccacctgga
     1561 aggcgtcgga gcggtggaat ctgcaggcgg acgcgacccg ttacgcggag tcccgccggc
     1621 gtcctgacgc cgcgggattc gactggaatc agacccgcct ctcggctcgg ctggtcctgt
     1681 tcttcgggag cgaggggcgc cccggcctgc cggcggccgt gcaggcgatg cccgaggcgc
     1741 cacgatgagg gccgcactgc tcttcacggc gatgctgctg gcggccggca cggcgtcggg
     1801 ccagacgccg caattcgatc acgccaagca cgcaaaggtg tttcccacct gtgtcgggtg
     1861 ccacgcgggc gcagccgagg tcaaggcggc cctctacccc gattccggtg tgtgcgcgtc
     1921 gtgtcacgac ggaaccatcc agaagcgtgt gccctacgtg ccgcccgatt cactgcgcac
     1981 caatcttcgc ttctcccacc tcggccatct ggccaaggtt ccggccgcgt cgtgca
//
LOCUS       DASUBP010000023        29600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1267865, whole genome shotgun sequence.
ACCESSION   DASUBP010000023 DASUBP010000000
VERSION     DASUBP010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 29600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29600
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267865"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            63..911
                     /locus_tag="VFU75_00925"
     CDS             63..911
                     /locus_tag="VFU75_00925"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5465420.1"
                     /translation="MLTINGLPLFTRRLGTGPLVVVLHGGPGASHDYLLPQYDLLSKD
                     RELFYYDQRGGGQSPAPREAPLGWEAHVSDLEEIRIALGQERLTICGYSWGGLLGLLY
                     TLRHPQRVERLALVAPAAITREWRDAFEAEFARRMNAPERLAERDELRASGLRERDPA
                     AYQRRAFELSVMGYFKNPADAVRLTPFRVVARTQKAVWDSLDDWDLRPDLERAVSSYP
                     RAHGPGFPVSRILHGTSDPIPIASSREISRITSADLIPLDCGHCPHVEVTSDFVRALD
                     EFLPRA"
     gene            930..2621
                     /locus_tag="VFU75_00930"
     CDS             930..2621
                     /locus_tag="VFU75_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEU5465421.1"
                     /translation="MDKPAVGQVLDEIARFLELKGENPFRVRAFENAARAVAGFPGDL
                     GEGVKSGALGLVHGIGKATLQIVSELLDTGRSSVLDELREQIPPGLVEMTRIPGLGVT
                     KIRQIHDALDIDTITDLDVAAADGRLAALPRFGAKTAENVRRGIAHLRRSTEFRLFHH
                     AEAEARSLRESLAAMEGVVRVEVAGSVRRRRELIRDLDFLIGYRDPGARDSIVKRLRT
                     TPGVTESSGAENAVTLRFASGTLADVFFAPVESFGLAWIRGTGSAAHLAQLETRASAR
                     SLSLAHGNYPEEADVYAALGLAWIPPELREGTGEIEAAASGTLPQLVEERDLRGFLHC
                     HSNYSDGTTTIADWAAACSAAGYSWMGLTDHGPGAFAGGLKAEDIGRQHDEIDAVNRA
                     DPSFSVLKGIEADILEDGKLDYDAATLDRFDFVIGSIHTRFGMTESQMTDRVLKAMDD
                     PHLTILGHPTGRLLLSRDPFPIDLEQVLRRAADRGIAIEVNADPHRLDLDWRHLREAR
                     DLGVTVSIGADAHSVAGMANVSVGVGIARKGWVAAEDVLNAREADGFRTFARARR"
     gene            2618..3271
                     /gene="nth"
                     /locus_tag="VFU75_00935"
     CDS             2618..3271
                     /gene="nth"
                     /locus_tag="VFU75_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEU5465422.1"
                     /translation="MKRKESQSEIRQRTHKIVGRLSREFPDAHCALDHRNAFELVAAT
                     ILSAQCTDARVNLVTPVLFKTFPTPEALADADQSAVEDIIKSTGFFRNKAKSLVGMAR
                     AVVERHGGQVPDTMDELVKLPGVGRKTANVVLGNAFGKNEGIVVDTHVQRISGLLKLT
                     REKTPEKIELALMPLVPRPKWTVFSHLLILHGRKTCIARRPLCGECRISDLCPSADL"
     gene            3268..4422
                     /locus_tag="VFU75_00940"
     CDS             3268..4422
                     /locus_tag="VFU75_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEU5465423.1"
                     /translation="MTIALTGIDRDALIATRRDLHQHPELGNQESRTSTLVAERLTAS
                     GYAVTTGVGNTGVVGVRGSGGRCILLRADMDALPVQEANEVPYKSRHAGKMHACGHDG
                     HVAIGLEVARRLGTAALPGTVKFAFQPAEEISDGAQAMIRAGVLDRPKVDAAFGLHLW
                     NDLPSGTIGIMPGPVMASVDEFDITIVGRGGHAAAPHQTIDPVLISAHLITALQSLIS
                     RRRDPFEEGVLSVTQVSAGHAFNVIPGTAELRGTVRTFGGRLWDEAPRLVEDTAKGVA
                     SAFGGTVNVRFRRHTRPLINDEAMSGLMRDVATEIVGAKNVRTGVRTMGGEDMSFFLA
                     SVPGCFAFVGSAAPNRPASPHHSPTFDIDESSLEIGVELLSRTAVRYLGA"
     gene            4422..6395
                     /locus_tag="VFU75_00945"
     CDS             4422..6395
                     /locus_tag="VFU75_00945"
                     /inference="COORDINATES: protein motif:HMM:NF015168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEU5465424.1"
                     /translation="MPNLLGAEASAYLKSAAHQPVSWEPWSERAFARAKSEDKPILLD
                     IGAVWCHWCHVMDGESYEDPTVAEVLNREFVCVKVDRDERPDVDARYQRAVQAISGQG
                     GWPLTAFLDSQGRVFYGGTYFPPDQNPYGRPGFRTTLVEIARRYREEREKLLNNATEL
                     AQRLGEALDETGEGEAREGVLVTGADQMARMFDVRYGGFGGAPKFPHPGACEFLLARW
                     WDTRDDWPREVVTRTLEGMSRGGIRDHLAGGFHRYAVDERWIVPHFEKMATDNAELLR
                     AYCSAYSAFGTPAFRVTASGIVDWILATLSDRTGHVFFASQDADTTFGDDGDYWTWTV
                     AEAKAALSGPEFDIAARVFDIGERGEMHLHPEKNVLWQRMPPSDTERATLETAIRNLK
                     SARDRRTAPFVDRTPYVSWNAMLAGALLQAGAVLDRAECNDLALGCLERLWRESWHEG
                     SGMSRVIGRAAPAGMLDDQVQSASAFLDAYEATGTDVWLDRAITVMEHVERAHGDSQG
                     GYWDISSTGGTAYLAVRGKPAQDAPSPSGNGVAALVLARLAALTDDAPWRGRLDRQVR
                     TFAGAAGQLGLHGATLLRAIDWAINPVTRIEVAGPLGPGEACAMHLAALQAWRPRRVV
                     IRRSGDRPQATVCVGTTCSLPVATRTALAELLA"
     gene            6392..7363
                     /locus_tag="VFU75_00950"
     CDS             6392..7363
                     /locus_tag="VFU75_00950"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEU5465425.1"
                     /translation="MNKRILGRTGFAVSEIGFGAWAIGGNKYGNSYGPTDDAESQRAV
                     RRALDLGCTFFDTADVYGHGHSEEVLGAALGADRSKVIVATKVGGNFYNRGVHALLHD
                     RIGQMLGSPYDTIPADAPLPVTHDSTFTPEYVRFALEQSLRRLGTDYVDLLQLHNPAL
                     NLIAAMDTYGVLEDLKQEGKIRAYGVSIHTPEEGLAAIQATMPDTIQVVYNIARREPE
                     DALLAAARAANVGIIAREPLANGFLAGRYGADATWEAGDIRARMPRPYVAKMAALGAR
                     VSELAAQSRVSPAHLALRFVLDNPDVGVVIVGTKTVKQVDDNFALEQ"
     gene            7363..7812
                     /locus_tag="VFU75_00955"
     CDS             7363..7812
                     /locus_tag="VFU75_00955"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU5465426.1"
                     /translation="MAATKAVIDTAQGVIELDLFSDEVPGTVANFEKLAKEGFYDGTT
                     FHRVIPNFMIQGGDPYSKTGKGRVGTGGPGYTIKCETHLNKHRHVAGTLSMAHAGKDT
                     GGSQFFICHSPQGHLDGVHTVFGQVTKGMDVVNKIKQNDKINSIKVS"
     gene            7812..8909
                     /locus_tag="VFU75_00960"
     CDS             7812..8909
                     /locus_tag="VFU75_00960"
                     /inference="COORDINATES: protein motif:HMM:NF022588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2911 domain-containing protein"
                     /protein_id="HEU5465427.1"
                     /translation="MRTLALAAVSLAALPLSAQDRGAWIARLGTDTVALETYQRTATG
                     IRGEIVTRSPIALHRIYTVTYGAGGWTYELVTHNIGGAPNVPAETKANGSGTPYLFPA
                     VGPIEDVVRSLKGDSATIEGVQMTNGRAIALSVKRVTRDSVRMYLGPIVGPFIGVLDS
                     AGNLASISGQQTTEKYDWERVRDLDLATLGPRFASRAVAILSPRETTTVAMGGTGITI
                     TYGSPSVRGRAIFGALVPWGQVWRAGANAATTFRTTGPVNVAGHLIPAGAYTLWILPT
                     PTAWTLVINRQTLAPCGATCPAQRAPLWGTDYSADSDLVRVPMLVQQVPSLIEHFGIT
                     ATPERGMTGGAALVTFAWEHTRASVELRKPK"
     gene            8906..9328
                     /locus_tag="VFU75_00965"
     CDS             8906..9328
                     /locus_tag="VFU75_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465428.1"
                     /translation="MNVDFAVVSDYALVDQAGKISVLGIFQHIWVQQFPAMHPRLHLV
                     LRLKGKRTEIGEHPVHIRLVDDRDTEILGGSGTVNFAEPPAGVTDIEAAAILVFDVPF
                     PHSGTYRFAISVDGAPAASVPITVSQMPAPPGGANPLH"
     gene            9338..11023
                     /locus_tag="VFU75_00970"
     CDS             9338..11023
                     /locus_tag="VFU75_00970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU5465429.1"
                     /translation="MPRPFSRLPWLLLIAAIALSWSLGGHRLLDPDEGRNAEVAREMA
                     QSGDYVVPHLDGLPYLDKPVFYFAAAAASMEALGPTEAAARLPALFFTLASVALVVWW
                     ARKRWGPEAGWLSGIALATMPLVFAFARETIFDSTLAFFLTAAILAFVEDRPVLAWAA
                     IGLGGLTKGPVAILVPLLAVVPYVLLTGRSLGRVFAWRGLAVFLLIALPWFLVVTARH
                     HEFPDYAFVRETFQRVTTKGFHRTAPFWYYIPIVLVGAFPWIVPALAQIGRPGRWRWL
                     WEARQVNQTAQDALLLLCWLIVPLVFFSLNQSKLPHYVLPLLPAIALAAGYGLSRQGL
                     ALGARAFIPVACLLGLGLITLTVWFPAPISLTPAEKAAMPTVALALGLVLVASGLTVA
                     LAIRRDTPSLGAAAYAAIVIAIPVFGGTLLRAVGEDRSSAPVADAVAAALSQSAAAGA
                     PAQVVGIGAFIPSLPFYLRRTIPVATATGKELTSTYIAEYADQFRTVPGSPLLTADAW
                     RDLLARCPLPTIFIARAGDRDVRATLHARLPLLAEDGRFTAYGPCRGGRTGGR"
     gene            11024..12847
                     /gene="asnB"
                     /locus_tag="VFU75_00975"
     CDS             11024..12847
                     /gene="asnB"
                     /locus_tag="VFU75_00975"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEU5465430.1"
                     /translation="MCGIFGVVSLTGRPLRHPECLEQMASSLAHRGPDGQRIVGHERV
                     RLGARRLAIMDLTTGDQPFQSPDGAIWMVCNGEIYNAPELRREAAGWGYPFRSTGDIE
                     TIVPHYERSGADVVRRLDGMFGLAIWDDAQRRLILARDRAGEKPLFWTVVDDELRFAS
                     EVQALLVFPDQARRIDRNAAGLYTALGYVPAPHSMIAGISKLPPGGLLEASAGGVTVR
                     SYWSAAEAAHGPDRLESAHQLRDALVGAVHRELMSDVPIGVFTSGGLDSSFLAAAAAR
                     AMPGQRIHTYAARFSAEGYDESDFAQAVTRDIHSIHHPVTADDVSLARAFEVVTSCLA
                     EPVGDPAILPTYLLAEAARADVKVVLSGEGADELFGGYPTHLGHKAAGVYRHIPGPFR
                     SLLTKAVERWPTSPGKVTIEFLLKQFVGSASRPLLERHAAWFGALGPTEDSVDAFARL
                     LRDFPAAPDLDRVLWLDFLTYLPDNLLVKVDRGTMLASIEARAPFLDREVLELVLPVP
                     AARKVRGWETKAILKEAAWGLVPAYVIDRRKRGLSVPVASWLNGALRDRAARYFTAPR
                     FGGERVTALWAEHREGKANHARKLWPMLMAEMWADRWKVTL"
     gene            12844..13419
                     /locus_tag="VFU75_00980"
     CDS             12844..13419
                     /locus_tag="VFU75_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU5465431.1"
                     /translation="MTREEALALMQEYTASPSLRQHMLAVEGAMRAYAVKSSEDVEAW
                     GLAGLLHDFDYERYPNDAHSATEEHPSWGVRLLRERGVGEPVCQAILGHADYTGVPRE
                     TALAKTLFAVDELCGFLVACALVRPSKSLTDLEVSSVKKKLKDKAFARGVNRDDVRRG
                     AEELGVPLDDHIAFVIGALRPIERTLGLGPV"
     gene            13425..14807
                     /locus_tag="VFU75_00985"
     CDS             13425..14807
                     /locus_tag="VFU75_00985"
                     /inference="COORDINATES: protein motif:HMM:NF024491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HEU5465432.1"
                     /translation="MHPGADGLARGLDRAAGTRPIPGNLITHHAASARALSAMLAMIG
                     EARRWVHLENYIIRDDVTGRRFADALSERAREGVRVRVLYDALGSRKTGRRYWQNLRH
                     AGAEVRPFNPLFDGRPFGWFRRDHRKLLVVDGNRAMVGGLCIGDEWAGDPARGRQPWR
                     DTALALRGPAAAALDSTFARVWRRAGTAIPEGELASDPEECGPTAVRIVEGVPEGARI
                     WRVVSLLATLSTERLWITDAYLVPPAPIVAGLVDAAHAGVDVRILVPGATDIPLVQAA
                     TRIGYRDLLRAGIRLFEWRGPMLHAKTLVADRRWVRVGSSNLNVSSLLANYELDVVVD
                     DESLATEMAEQHRRDAAASSEVVLVPRRRLPPRLTNAQAEAPTVAHQPTARERAKTAV
                     VALRQVAGGLRRRLAVAASGGAALLGALLLGFPHVTSITLAAGAFAAAFTFGWYALVR
                     RARERGQDVP"
     gene            14797..15384
                     /locus_tag="VFU75_00990"
     CDS             14797..15384
                     /locus_tag="VFU75_00990"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU5465433.1"
                     /translation="MSPDSQPRAGGARIEHREEAELVTRSLAGDRAAFGALVDKYAAQ
                     ARRVARAVLRDPDDADDAAQDAFLSALVKLEQYDPRRPFGPWLMRIVANAATDRRRRR
                     SVRSAEPLDPGVVGGGPRPDTAAERSALSERLRQALAELPERRRLAVVLFDVEGYSHA
                     EIAGMLKIPEGTVRSEVFHARRKLRTLLEDWREPS"
     gene            15381..15737
                     /locus_tag="VFU75_00995"
     CDS             15381..15737
                     /locus_tag="VFU75_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465434.1"
                     /translation="MMEELPFDHEQDARLGAALREALAPAGHEAFVARVMAGVSTTKA
                     GSWDVLAGWAGRGIAAALLVALGAGFAAGRAMQPSNPSIEEGLVSAPADSGTAAAALV
                     RGNNPPDASIVFAVRP"
     gene            15742..16131
                     /locus_tag="VFU75_01000"
     CDS             15742..16131
                     /locus_tag="VFU75_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465435.1"
                     /translation="MFNRSKAQALALLTAVFVVGGAAGWELDAWAHRPRPHGPEAFVN
                     YLTGELKLDSLQRDSVRAVLVRHKPQMDSIWKDVHTRVDSLRHVMRGEIFTHLTDAQR
                     TKYLQLVAEQAHQRGDSTRDTTHGGRN"
     gene            16139..17431
                     /locus_tag="VFU75_01005"
     CDS             16139..17431
                     /locus_tag="VFU75_01005"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU5465436.1"
                     /translation="MSILITGLVVSGGLASAQTPSQAPDSGPPTVTLTDAVQRALTVQ
                     PAMVSAIGSQRNASASRTSAFGTFLPSVTAGWSASKSSQSRFDNNRSVLIPPTWNYGF
                     NINASVDLFDGLRRIWNVKSATAVQDAADAGYISQRFVTELQTKQAFFAALADQELLR
                     VAQAQLQRTEQELKAATDKLRFGAATRADSLTAAVDHGNAEVALLQAQANLVGAQANL
                     ARQIGVNGLVQPVADSTLPPLPDTTGFLRSVIENAPLVQQADAQATAARANVLSARSQ
                     YLPTFNVSFGDNRSDTTFGGGFLHGHVTRWTFGASFPLFNGFVREQNQVSASVNRDVA
                     TATAADTRRNVAAQLAQQVAALVAAYHQIEFARSNRASAAEALRVQQQRYAAGAGLLL
                     DLLTAEANLTQAEATLIQARFTYRTTRATIESLVGKEL"
     gene            17428..18174
                     /locus_tag="VFU75_01010"
     CDS             17428..18174
                     /locus_tag="VFU75_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5465437.1"
                     /translation="MIATASPMQEFRTGDTPLPDVVILTHRLTREYVIGSETVRALRG
                     VDLQIRKNEFVAIMGPSGSGKSTFMNMIGCLDTPTDGEYWLNGQKVSELQDDSLARIR
                     NKEIGFVFQTFNLLPRANALHNVELPLIYAGETSRARKERAAAALAKVGLGDRMDHRP
                     NELSGGQRQRVAIARALVNDPSILLADEPTGNLDSATGNDIMRLFEELWEAGQTIVLV
                     THEPDIAARAHRQIHLLDGKIERDERTESK"
     gene            18171..19697
                     /locus_tag="VFU75_01015"
     CDS             18171..19697
                     /locus_tag="VFU75_01015"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5465438.1"
                     /translation="MKRIVLVGGLAALGLAGCKKAAQSPVYIAVPVERRNIVVAAAAA
                     GPIQPILTVSVKSQASGAVTEMHVQTGDDVKPGQLLALIDPRLPKSALDQAQATVDQA
                     QASFDYAKAQLAREDTLYKVQAVTQQEYQSTKVSYEQARAALINAQANLSDAKIAYDQ
                     TRVTATLAGTIILKEVDIGTVISSPTHDVTGGTEMFQMANLDTVQVQAMVDETDVGKV
                     QAGQPVTITVDAYPSRPFQGNVLKVEPKATVSQNVTQFPVDIDILNPGHLLKPGMNTE
                     VEIHVGQRDSVLAIPTSALRTQKDVASAASVLGMDPNVVMQQLAAATPKPAPNTKGDT
                     AASMGNSAGAGKGTMTMPDGRVVKLPQGVTAAQVQGIFDKMRSGGFSSITAADRDVMT
                     KLRQANPDMMGGGRGRGGRGGPGGPVGGPVGNGTQSNSYIVFALRKGQPTAVSIKTGL
                     SDLDYVEITSGLTEGDTVLLLPSASLVQSQAQFKQRMQGITGGGLPGVRQTPQASGAR
                     "
     gene            19700..20911
                     /locus_tag="VFU75_01020"
     CDS             19700..20911
                     /locus_tag="VFU75_01020"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5465439.1"
                     /translation="MLLGEIIKVALQAIRANKLRALLTMLGIIIGVGAVITMVALGTG
                     AQKSVQDRIQALGPTLLTVFPGQSFRGGVAFSTRVSLTMDDDTALLNNRHYISEVVPE
                     LQGNLQVQYANQNINTTVLGSTANYYSVHNYNMIAGRMFTNGEDDARQRYAVLGGQVP
                     EELNANPQAMIGQQLMIRGIPFEIIGVLSNKGSQGFSNPDEQILIPLQTARYRIFGSN
                     RIRDLTVEVGDLANMNLGMIEIESILRRQHKIRPGMDNDFQIRNQTDILSTFQETAQT
                     FGFLLAGIATVSLIVGGIGIMNIMLVSVTERTREIGVRKALGATRFNILFQFLVEALV
                     LCMAGGIIGIILGSGGAIVLAQFAHWNTSISLYAIVLAFFFSAIVGLLFGVWPARRAA
                     SLDPIEALRYE"
     gene            21010..23325
                     /locus_tag="VFU75_01025"
     CDS             21010..23325
                     /locus_tag="VFU75_01025"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding family 9 domain-containing
                     protein"
                     /protein_id="HEU5465440.1"
                     /translation="MLFSLLLALQGAQTPDPHPVFAGRSGALNVAIPRVDAKVTVDGN
                     LDEPVWNQAALLTGFSQYTPVDGLPAADSTQVLVWYGSDAIYFGIRAYEPHGDLVRAT
                     MADRDAIDADDNIQILLDTYNDHRRALEFAVNPLGVQEDGVRSEGLAGAAGGGNAATG
                     RFDGVIDLNPDFVWESRGHVTPWGYQVEVRIPFKSIRYQSVDPQSWGLQVIRYVTHSG
                     YQDTWTPVVRANASFLIQAGTLTGLSDLHRGLVMDVTPEFTSKVEGDSAGAGRYLYKG
                     TPEIGGTLHWGLTSNLSLAATAHPDFSQVEADAAQVTVNQRFALFYPEKRPFFLDGLE
                     QYDTPNQLIYTRQIIQPVAGAKLTGKAGGTNIAYLGAVDAVDPATGKNPVYDVMRIST
                     DLGASSTAGFAYTDRVEGTDYNRVFGADSRVLWGTGWFSQAQIAGSWTRDASGVRSGT
                     LWDATLGDYTGRRYGNHFEFLGSTPNFRASSGFVNRTGIVSARLFNRFTWYGKPGALM
                     EQLQTIVMGTPLWRYDDFWHARGTIEGGWANSWGLTLRGGWNVSAAVNDNLQRFDSAT
                     YAGYQVNRGIDTIPFTIPHGLYNLLSANAGITSPYRAFQWSVSAGYGHSVIFAEAAEG
                     KELTTSASIAWKPTSAIRVSATWLHDRIVRERDGSEFALANIPRLEFDYQLSRSVFVR
                     YIGQYVAQRRSALQDPRTGQPLLVDSNAVVGSLSNGFRNDFLLSYKPIPGTVFFLGYG
                     TSLSEPTAFGFSNLQRVQDGFFLKASYLFRM"
     gene            complement(23333..23869)
                     /locus_tag="VFU75_01030"
     CDS             complement(23333..23869)
                     /locus_tag="VFU75_01030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465441.1"
                     /translation="MKSATALLLGLFAFGCRPHAPLAHNRRGLQRLPGVHGRDSLELR
                     DSSRSVLRRPRVITAPTMIVFWLGAADTLSAGDASSAYDDLTYYTDQVAAELRQAGVA
                     IVPTTADTVYVEGPGHRRRMIVLSGLDYPYGYVLLDPGGPERILTGIYSDDDLLDEID
                     AYFNIDSDSTAAPPRVAT"
     gene            23891..24568
                     /locus_tag="VFU75_01035"
     CDS             23891..24568
                     /locus_tag="VFU75_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HEU5465442.1"
                     /translation="MTTSAPAPVRRHHWIVRLCHWATVFVLAGLVASGLQIYEAYSRF
                     GNRGDPGYPSPFNGTVFPHWSRLGGWLAAGLNWHFALMWPFALGGLLYLSYLAFSGEW
                     RSLLFRPRDIRGAIEMQKYYLGIRKDHPAQGKHNPLQKLAYTFIILLGTLSVLTGLSI
                     YKTTQFSWLTSLFGGFQAARYWHFWAVWIFVGFTLTHVALVFLVDPHSLRAMITGWYR
                     GRFPSHD"
     gene            24561..25262
                     /locus_tag="VFU75_01040"
     CDS             24561..25262
                     /locus_tag="VFU75_01040"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU5465443.1"
                     /translation="MTDRRSFFKTAGISAASLLAGCGWDGGKSVKPALLSISRLNDWV
                     GEHLLYSPSRLAKNYDPSRRSVGLPAYFISDTVPMLQDPAAWRLSVGGLVRTPLNLTL
                     DDIKRMPSVTYTVKHHCVEGWSAIATWTGVPVSAIARQSGMTSSAHYIDFQSFDSGYS
                     NGWDLASAMHPQTILAYAMNDRLLGPDHGAPLRLYSPTKLGYKMTKYLTAMNFTEQRP
                     GGYWEDQGYPWFGGL"
     gene            complement(25398..27437)
                     /locus_tag="VFU75_01045"
     CDS             complement(25398..27437)
                     /locus_tag="VFU75_01045"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU5465444.1"
                     /translation="MRDASSTDKGPRFGLAWKVFAASVVLIVAVLGATLVYTSWQANR
                     AADVSIRKALQNTHRAVDAQLEARTRAMAAAATAAASVPQYRTRLLQGQKRADVLDQA
                     NEIRNLAGAAWAMVTNSDGLLIARTDQPAADSIDYSNSVLFSNALSGDQTTGAFGDLQ
                     THSLYYAVAIPLRASPQDAPQGVLVVAYPFDDALAAAIKEATQTDLVFFSVDTLGRPY
                     PVGATVPAAELLPVLAANIGADTSADTTGMEMAADMSGSHYIGLEEKILNAGGRRLGG
                     WVALRSRDQEMATFRTLKRALYYAFGVGFVLALVVASIGASSIAKPVRRLAEATRKVR
                     DGDYSVDVATSSNDEIGMLSRDFRDLVEDLKEKSDLVEYMMSATGAAATQPISAVPTA
                     VRAAAAASSGTMLSPGALFAGRYEVKEVLGMGGMGVVYRAFDRELQEPVAIKTLRPEA
                     MSADAVALDRFKQEIRLARKISHRNVVRTYDLGEVKGTYYLTMEFVEGKSLKSLIEGR
                     GRLPIPVTLTIGKQLCRALEVAHEQGVIHRDIKPQNMVVEPSGFLKVMDFGIARLANL
                     PKGKGLTEAGMSIGTPDYMSPEQLQGLDLDARSDLYSAGVVLFECVSGRVPFEADTPW
                     ALIAKHLEENAPDPRTLNPEVPETLAAVILKSMGRKPGDRYQSAASMYDALDLIG"
     gene            complement(27415..28104)
                     /locus_tag="VFU75_01050"
     CDS             complement(27415..28104)
                     /locus_tag="VFU75_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU5465445.1"
                     /translation="MRHLRLTFVMGVLAAPGALSQTVPASRVSGTIAILEKHNKPSPD
                     IGDAVIYLTGGNESAGRPVALDVTISDKKFVPDVIVVPVGSTVRFPNTDPFSHNVFSA
                     SDPNGFDLGLYGRGEAKGHVFDHAGLVYIYCNVHSSMVAYVQVMASRWFTQPGADGSF
                     SISGVPAGRYTLHAWHPRVSQEVVQDVEVPAAGLTLAQPVQLDARGFRQRAHKNKYGK
                     PYPTNAGRELY"
     gene            28183..29361
                     /locus_tag="VFU75_01055"
     CDS             28183..29361
                     /locus_tag="VFU75_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465446.1"
                     /translation="MKRILAVLTILTAPAAAQQSKPAPPQRTVQLTGMVLVNGFWNSS
                     QVSNVDLPLAAVPDSVGVKGGGASIRQTRIGVLVNDPDVLGGTFTGEIDADFYGGQQP
                     SGSGRTSPLLRLRRATGEIDWSHVQLMVGQEKPLVSPRNPRSLAAVGFPEFSGSGNLW
                     LWIPQMRVTVETGYSLRLALQAAVIAPAAGTAQPAFNTSLDSAERTGRPYLEGRLRFG
                     WGAADNPSEIGIGVHQGWLKNLDSLSGDSIQVSQAVTADARFLFGPFELIGEFFTGKG
                     LAGLGGGGVGQNVGVDGAVVHTTGGWGQFNARPMKDWMFGVGCGIDDPKDADVYTVGD
                     STYLTKGGRLKNLACEGHLQWTPPGPVVLGFEFRQIRTTYLTGEERVNHLNLAIGYRF
                     "
     gene            complement(29363..>29600)
                     /locus_tag="VFU75_01060"
     CDS             complement(29363..>29600)
                     /locus_tag="VFU75_01060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465447.1"
                     /translation="GDLFELGYEAVGDLDARLETVSDWKAPFREGVVYFLGEGRVKGV
                     LLWNTWGQVEHARALIAEPGPFTPATLVGRLPVA"
BASE COUNT         4857 a         9996 c         9841 g         4906 t
ORIGIN      
        1 agggattgcc cgggggcggc cacggccgcc cccgtttttc gtccggtagt taggttctcc
       61 gcgtgctcac gatcaacggc cttcctctct ttacgcgtcg cctcggcacc ggccccctcg
      121 tggtcgtgct ccacggcggt ccgggcgcca gccacgacta cctccttcct caatacgacc
      181 tcctgtccaa agaccgggag ctcttctact acgatcagcg cggtggtggc cagtccccag
      241 cgccccgcga agcgcccctc ggctgggaag cccacgtctc cgacctcgaa gagatccgga
      301 tagcgctggg ccaggagcgg ctgaccatct gcggatactc gtggggaggg ctgctcggtc
      361 tcctgtacac cttgcgccat ccccagcgcg tcgagcgcct ggcgctggtc gctcccgccg
      421 ccatcacccg ggaatggcgg gacgcattcg aagccgagtt cgcccggcgg atgaacgcgc
      481 ccgaacggct ggccgagcgt gacgagctgc gtgcgtcggg cctccgggag cgcgaccccg
      541 cagcctatca acgccgcgcc ttcgagctgt ccgtgatggg ttatttcaag aatccggccg
      601 acgcggttcg cctcaccccc ttccgggtcg tcgcgagaac ccagaaggcc gtgtgggact
      661 ctctggatga ctgggacctt cgccccgact tggagagagc cgtgtcgagt tatccccggg
      721 cccatggccc ggggtttccc gtttcccgca ttctccatgg gacgtcggat cccatcccga
      781 tcgcgtcctc gcgggagatt tcacgcatca cctccgcgga cctgatcccc ctcgactgcg
      841 gccactgccc gcacgtcgaa gtcacgtccg atttcgttcg cgcactggac gaattcctgc
      901 cccgggccta gcgggttagc ttcccgaaca tggacaagcc ggcggtgggc caggtactcg
      961 acgagatcgc ccgcttcctg gaattgaagg gcgagaaccc atttcgcgtc cgcgcgttcg
     1021 agaacgccgc gcgggcggtg gccgggttcc ccggggacct gggcgagggc gtgaagtcgg
     1081 gcgcgctcgg ccttgtccac gggatcggaa aagccactct gcaaatcgtc tccgagctcc
     1141 tcgataccgg ccgttcctcg gttctggatg agctgcgcga gcagatcccg cccggactgg
     1201 tcgagatgac ccggatcccg ggcctgggcg tgaccaagat ccgccagatc cacgacgcgc
     1261 tcgacatcga caccattacc gacctcgacg tcgccgccgc cgacggccgc ctcgccgccc
     1321 tgccgcgctt cggcgccaag accgccgaga acgtgcggcg tgggatcgcc cacctgcgcc
     1381 gctctactga gttccggctc tttcaccacg cggaagcgga agcgcgctcc ctccgggaga
     1441 gtctcgccgc gatggaaggg gtcgtccggg tcgaggtggc agggagcgtg cgccggaggc
     1501 gggagctcat ccgggacctc gacttcctga tcggctaccg cgaccccggc gcccgggatt
     1561 cgatcgtgaa acgcctgcga accacgccgg gcgtcaccga gtcgtccggg gcggagaatg
     1621 cggtcacgct gcgcttcgcg tccggcacac tggccgacgt gttctttgcg ccagtggaat
     1681 ccttcgggct cgcgtggatc cggggcaccg gcagtgcggc gcacctggcg cagctcgaga
     1741 cccgtgccag cgctcgcagc ctgtctctcg cgcacggcaa ttatccggaa gaggcggatg
     1801 tgtacgcggc actgggcctc gcctggattc cccccgagct cagagaggga accggcgaga
     1861 tcgaagcggc ggcgtccgga accctgcccc agctcgtgga agagcgggac ctgcgcgggt
     1921 tcctccattg ccacagcaat tactcggacg gcaccaccac catcgccgat tgggctgccg
     1981 cctgctcggc cgccggctac tcgtggatgg gcctgaccga tcacggtccg ggggcgttcg
     2041 cgggtggcct gaaggccgag gacatcgggc gccagcacga cgaaatcgac gccgtgaacc
     2101 gtgcggaccc gtcgttctcg gtgctgaagg gcatcgaagc cgacatcctc gaggacggca
     2161 agctcgacta cgacgcggcg acgctcgacc gcttcgactt cgtcatcgga tcgatccaca
     2221 cccgcttcgg catgaccgag tcgcagatga cggaccgcgt gctgaaggcg atggatgacc
     2281 cgcacctgac cattcttggg caccccacgg gacggctgct cctgtcccgc gatcccttcc
     2341 ccatcgatct cgagcaggtg ctgcggcggg ccgccgatcg cggcatcgcc atcgaagtga
     2401 acgccgaccc gcaccggctc gacctcgact ggcggcacct gcgggaggcg cgcgacctcg
     2461 gcgtcacggt gtcgatcggc gccgacgcgc actcggtcgc gggaatggcg aatgtcagcg
     2521 tgggggtcgg catcgcgcgc aaagggtggg tcgccgccga ggacgtcctc aacgcgcggg
     2581 aagccgacgg cttccggacg ttcgcgaggg cacgtcgatg aagcgcaagg aatcccagag
     2641 cgagatcagg cagcggacgc acaagatcgt gggccgcctc tcgcgtgaat tccccgacgc
     2701 gcactgcgcc ctcgaccatc gcaacgcctt cgagctcgtc gcggccacca ttctctcggc
     2761 gcaatgcacc gacgcgcggg tgaacctggt gacgccggtg ctgttcaaga cgttcccgac
     2821 tccggaagcg ctcgcggacg ccgatcaatc cgcggtggag gacatcatca agagcacggg
     2881 gttcttccgg aacaaggcga aatccctggt ggggatggcc cgggccgtgg tcgagcgcca
     2941 tggcggccag gtacccgaca ccatggacga gctggtcaag ctcccgggcg tcgggcgcaa
     3001 gaccgcgaac gtcgtcctgg ggaacgcctt cgggaagaac gaagggatcg tcgtggatac
     3061 tcacgtccag cgcatttcgg gactgctcaa gctcactcgc gagaagacgc cggagaaaat
     3121 cgagctcgcg ttgatgcccc tggtgccccg ccccaagtgg accgtgttct cgcacctgct
     3181 catccttcat ggccggaaga cctgcatcgc ccggcgcccg ctctgcggcg aatgccggat
     3241 ctccgatctc tgcccgagcg ccgacctgtg accatcgccc tgaccggaat cgaccgcgac
     3301 gccctcatcg cgacccgacg cgacctccac cagcaccccg agctggggaa tcaggaaagc
     3361 cggaccagca ccctggtcgc cgagcggctc acggcgtcgg gctatgcggt gacgacgggt
     3421 gtcgggaaca ccggagtggt cggggtgcgg gggtcggggg gccggtgcat cctcctgcgc
     3481 gcggacatgg atgccctgcc cgtgcaggag gcgaacgaag tcccctacaa gtccagacac
     3541 gccgggaaaa tgcacgcctg cggccacgat gggcacgtcg ccatcggtct cgaagtcgcg
     3601 cggcggctgg ggaccgcggc gctccccggg acggtgaagt tcgcgttcca acccgccgag
     3661 gagatctccg acggcgcgca ggcgatgatc cgcgccggcg tgctcgaccg tcccaaggtc
     3721 gacgccgcgt tcggcctgca tctctggaac gacctcccgt cggggaccat cgggatcatg
     3781 cccgggccgg tgatggcgtc cgtcgatgag ttcgacatca ccatcgtcgg acgtggcggc
     3841 cacgccgcgg ccccgcatca gaccatcgac cccgtcctca tctcggccca cctgatcacg
     3901 gccctccaaa gcctgatctc ccgccggcga gatcccttcg aagagggcgt gctgtcggtc
     3961 acccaggtct cggcgggtca cgcgttcaac gtcattcccg ggaccgcgga gctgagaggc
     4021 acggtgcgga ccttcggcgg ccgtctgtgg gacgaagcgc cgaggctcgt cgaagacacc
     4081 gcgaagggcg tagcttccgc cttcggtggc accgtcaacg tgcggttccg ccgtcacacc
     4141 aggccgctga tcaatgacga ggcgatgtcc ggcctgatgc gcgacgtggc gaccgagatc
     4201 gtcggcgcga agaacgtccg cacgggcgta cggaccatgg gcggcgagga catgtcattc
     4261 ttcctcgcca gtgtgccggg gtgcttcgcg ttcgtcggct ccgccgcgcc gaaccgcccg
     4321 gcgtcgcccc accacagccc gaccttcgac atcgacgagt cgtccctcga gatcggtgtc
     4381 gagcttctgt cccgtacggc cgtacggtac ctcggggcgt agtgccgaat cttctcggcg
     4441 ccgaggcgtc ggcgtacctc aagtcggcgg cccaccaacc ggtctcgtgg gagccctgga
     4501 gcgagcgcgc gttcgcgcgc gcgaaatccg aggacaagcc cattctgctc gacatcggcg
     4561 cggtgtggtg ccactggtgc cacgtgatgg acggcgaatc gtacgaggac cccaccgtcg
     4621 ccgaggtatt gaaccgcgag ttcgtctgcg tgaaggtgga tcgggatgaa cgccccgacg
     4681 tcgacgcccg ctatcaacgc gcggtgcagg cgatctccgg acagggtggc tggcccctca
     4741 ccgcattcct cgattcgcaa ggccgggtgt tttacggtgg cacctacttt ccacccgatc
     4801 agaaccctta tggccgtccc ggattccgga ccaccctggt cgaaatcgcg cgacggtatc
     4861 gggaggagcg cgagaagctg ctgaacaacg ccacggaatt ggcccagcgc ctcggcgaag
     4921 cgctggatga aaccggcgag ggcgaggccc gagagggcgt gctcgtgacc ggtgccgatc
     4981 agatggcccg catgttcgac gtgcggtatg gcgggttcgg cggcgcaccc aagttcccgc
     5041 accccggcgc ctgtgagttt ctcctcgcgc gctggtggga tacccgggac gactggcccc
     5101 gggaagtcgt gaccaggacc ctggagggca tgtcgcgggg cggcatccgc gatcacctcg
     5161 ccggcggctt ccaccggtac gcggtcgacg agcgctggat cgtcccgcac ttcgagaaaa
     5221 tggcgaccga caacgccgag ctcctgcgcg cgtactgctc cgcctactcc gcgttcggta
     5281 ctcccgcgtt ccgggtcacg gcttcaggga tcgtggactg gattctggcg acgctcagcg
     5341 atcgcaccgg gcacgtgttc ttcgcgtcac aggacgccga cacgaccttc ggcgacgatg
     5401 gcgactactg gacgtggacc gtagcggaag cgaaagccgc gctgtcaggt ccggagttcg
     5461 acatcgccgc gagggtcttc gacatcggcg agcggggtga gatgcatttg caccccgaga
     5521 agaacgtcct ctggcaacgg atgccgccgt cggacaccga gcgggcgacc ctggagaccg
     5581 cgatacggaa cctcaagagc gcacgcgacc ggcggaccgc gccctttgtg gaccgcacgc
     5641 cttacgtatc atggaacgcg atgctggctg gggcactcct gcaagcgggc gcggtgctgg
     5701 accgcgccga gtgcaacgac ttggccctgg gttgtctgga aagactctgg cgggagagtt
     5761 ggcacgaggg gagtggcatg tcgcgggtca tcggacgcgc tgcgccggcg ggaatgctcg
     5821 acgaccaggt gcaatcggcg tccgcatttc tggatgcgta cgaagcgacg gggacggacg
     5881 tttggctcga tcgggccatc acggtgatgg agcacgttga gcgcgctcac ggcgacagcc
     5941 agggcggtta ctgggacatc tccagcacgg gcggcacggc gtacctggcc gtccggggga
     6001 agccggcgca agacgcgccg tcgccgtcgg ggaatggtgt ggcagcgctc gtgctcgcgc
     6061 gactcgccgc cctcacggat gacgcgccgt ggcgtggccg tctcgaccgg caagtgcgga
     6121 cgttcgccgg tgccgcgggt caactcggcc tccatggcgc cacgctcctg cgagccatcg
     6181 actgggccat caatcccgtc acgcgaatcg aggttgccgg ccccctgggg cccggtgagg
     6241 cgtgcgccat gcatctcgcc gcgttgcagg cctggcggcc gcgccgcgtg gtgatcaggc
     6301 gctccggcga ccgtccacag gccaccgtct gcgtcgggac gacctgttcc ctgccggtcg
     6361 cgaccaggac cgcccttgcc gagctgctcg cgtgaacaag cgcatcctgg gccgcaccgg
     6421 attcgccgtt tcggagatcg gattcggtgc ctgggccatc ggcggcaaca aatacgggaa
     6481 ctcctacggc cccaccgatg acgcggagtc tcaacgggcg gtccgcaggg cgctcgatct
     6541 ggggtgcacg ttcttcgaca ccgccgacgt gtacggtcac gggcacagcg aggaagtcct
     6601 cggcgcggca ctcggcgccg accgttcgaa agtgatcgtt gcaacgaagg tgggtggcaa
     6661 tttctacaac cgcggtgttc acgcgctcct ccacgaccgc atcggccaga tgctggggtc
     6721 gccatacgac accatccccg ccgacgctcc gctccccgtc acccacgatt ccacattcac
     6781 tccggagtac gtccgcttcg cgttggagca gagcctgcgg cgactgggaa cagactatgt
     6841 ggacctgctg cagcttcaca atccagccct gaacctcatc gcggcgatgg acacgtacgg
     6901 agtgctcgag gatctcaagc aggaaggcaa gatccgggcg tacggagtct cgattcatac
     6961 gcccgaggag ggattggccg ccattcaggc gaccatgccc gacaccatcc aggtcgtgta
     7021 caacatcgcg cggcgcgagc ccgaggacgc cctgctcgcc gcggcgcggg cggccaacgt
     7081 cggcatcatc gcccgggagc ccctggccaa tggatttctc gcgggaaggt acggagcgga
     7141 tgcgacctgg gaagcgggag acatcagggc ccgcatgccc cggccttacg tagcgaaaat
     7201 ggcggcgctg ggggcccggg tgtcggagct cgcggcgcag tcgcgggtct caccggctca
     7261 cctggccctg cggttcgtgc tggacaaccc ggacgtgggc gtcgtgattg tagggacgaa
     7321 aaccgtgaag caggtcgacg acaacttcgc actggagcag taatggcagc cacgaaagcc
     7381 gtaatcgata ccgcccaggg tgtgatcgag ctggacctct tttccgacga ggtgcccggc
     7441 acggtcgcga acttcgaaaa gctggccaag gaagggttct acgatgggac cacgttccac
     7501 cgggtgattc ccaacttcat gatccagggc ggcgatccgt actccaagac cggcaaagga
     7561 cgcgtcggca ccggggggcc cggctacacc atcaagtgtg agacccacct caacaagcac
     7621 cggcatgtcg ccggcacgct ctccatggcg cacgcgggca aggacaccgg cggttctcag
     7681 ttcttcattt gtcattcgcc gcagggtcac ctggacggcg tgcacaccgt gttcgggcaa
     7741 gtcaccaagg ggatggacgt cgtcaacaag atcaagcaga acgacaagat caactcgatc
     7801 aaggtgtcgt gatgcggaca ctcgcactcg ccgccgtgag cctggccgca ctccccctgt
     7861 ccgctcagga ccgcggcgcc tggatcgcgc gcctcggcac cgacacggtg gcactcgaga
     7921 cctaccagcg cacggccacg ggcatccgtg gtgagatcgt cacgcggtcg cccattgcgc
     7981 tgcaccgcat ctacacggtg acgtatggcg cgggcgggtg gacctacgag ctggtgacgc
     8041 acaacatcgg cggcgcaccg aacgtgccgg ccgaaaccaa ggccaatggc tcgggcacgc
     8101 cctacctctt tccggccgtc ggccccatcg aggacgtcgt tcggtccctg aagggcgatt
     8161 ccgccaccat tgaaggcgtg cagatgacga acggaagggc gatcgcactg tcggtgaagc
     8221 gagtgaccag ggattcggtc cggatgtacc tggggcccat cgtcggacca ttcatcggcg
     8281 tgctcgacag tgcgggcaat cttgcgagta tctccggaca gcagacgacc gagaagtacg
     8341 actgggagcg agtgcgagac ttggacctcg ccacgctcgg tcccaggttc gcgagccgcg
     8401 ccgtcgccat cctctcgccg cgcgagacga ccacggtggc gatgggcggt accggaatca
     8461 ccatcaccta cggcagtccc tcggtccgag ggcgcgccat cttcggcgcg ctcgttccct
     8521 ggggtcaggt atggcgcgcg ggcgcgaacg ccgcgaccac tttcaggacc acgggccccg
     8581 tgaacgtcgc cggtcacctg attccggcgg gcgcgtacac gctgtggatc ctgcccacgc
     8641 cgacggcgtg gaccctggtg atcaaccggc agacgctggc tccctgcggt gccacgtgtc
     8701 ccgctcagcg ggcccctctg tggggaacgg actactcggc cgactcggat ttggtccgcg
     8761 tccccatgct ggtccagcag gtcccctcgc tcatcgagca cttcgggatc accgccactc
     8821 ccgaacgcgg gatgacgggc ggcgccgccc tcgtcacgtt cgcctgggag cacaccaggg
     8881 cgtcggtcga gctccggaag cccaagtgaa cgtcgacttc gcggtcgtct ccgactacgc
     8941 cctggtcgac caggcgggca agatctcggt cctcggcatc ttccagcaca tctgggtcca
     9001 gcaattcccg gccatgcacc cgcgcctgca tctcgtcctg cggctcaaag gcaagcggac
     9061 ggagatcggc gagcaccccg tccacatccg cctcgtggat gaccgggaca cggagatcct
     9121 gggcggctcc ggcaccgtca acttcgcgga gcctccggcc ggggtcacgg atatcgaagc
     9181 cgccgccatc ctggtcttcg atgtgccatt tccgcattcg gggacctacc gtttcgccat
     9241 ttccgttgac ggcgcgccgg cggcatcggt ccccatcacg gtgagtcaga tgcccgcacc
     9301 gcccggcgga gccaacccgc tgcattagat tcaccgcgtg ccacgaccat tttcacggct
     9361 cccctggctc ctgctgatcg ccgcgatcgc gctcagctgg agcctcggcg ggcatcgcct
     9421 cctcgaccct gacgagggac ggaacgccga agtggcgcgt gagatggcgc agtcggggga
     9481 ttacgtggtc ccccacctcg acggccttcc gtacctcgac aagccggtct tctatttcgc
     9541 cgccgccgcg gcgtcgatgg aggcactcgg cccgaccgaa gcggccgccc gcctccccgc
     9601 gctgttcttc accctcgcat ccgtcgcgtt ggtggtctgg tgggcccgca agcgctgggg
     9661 tccggaggcg gggtggctct ccggcatcgc gctcgccacc atgccgctgg tctttgcctt
     9721 cgcgcgcgag accatcttcg acagcacgct cgcattcttc ctcactgccg cgatcctcgc
     9781 cttcgtcgag gaccggccgg tgctggcctg ggccgcgatc ggactcgggg ggctgaccaa
     9841 gggaccggtg gccattctcg ttccgttgct cgccgtcgtg ccctacgtgc tcctcaccgg
     9901 acggtccctc gggcgcgtat tcgcatggag gggactggcg gtctttcttc tcatcgcgct
     9961 gccctggttc ctggtggtga cggcgcgtca tcacgagttc cccgactacg cgttcgtgcg
    10021 cgagaccttc cagcgggtca ccaccaaggg gttccatcgg accgccccgt tctggtacta
    10081 catcccaatc gttctggtcg gcgcgttccc ctggatcgtt cccgccctgg cgcagattgg
    10141 ccgtccgggt cgctggcgct ggctctggga agcccgtcag gtgaaccaaa cggcgcagga
    10201 cgccctgctg ctgttgtgct ggttgatcgt gcccttggtg ttcttctcgc tcaaccagtc
    10261 aaagctgccg cactacgtgt tgccactgct tccggccatc gcgctcgcgg cgggatacgg
    10321 actctcccgg caggggctgg cgctcggcgc cagggctttc attccggttg cgtgcctcct
    10381 cggactcgga ctgatcacgc tgaccgtgtg gttccccgct ccgatctccc tgacgcccgc
    10441 cgagaaggcc gcgatgccca ccgtcgcgct cgccctcggc ctggtactgg tggcgtccgg
    10501 actcacggtc gccctggcga tccggcgtga cactccatcg ctgggcgccg ccgcgtacgc
    10561 cgcgatcgtc atcgcgattc cggtcttcgg gggaaccttg cttcgcgcgg tcggcgagga
    10621 tcgctcgtcc gctcccgtcg ccgacgcggt cgccgcggcc ctgtcgcagt ccgctgcggc
    10681 cggcgcgccc gcgcaggtcg tgggcatcgg cgccttcatt ccctctctgc cgttctattt
    10741 gcggcggacc atccccgtcg cgaccgcgac gggcaaagag ctgacgagca cctacatcgc
    10801 cgagtacgcc gaccagttcc gcaccgtacc cggatcgccg ctcctcaccg ccgacgcctg
    10861 gcgcgatctt ctggcgcggt gtcctctgcc gacgatcttc atcgcccgcg ccggcgatcg
    10921 ggacgtgcgc gccacactcc atgcccgcct tccgctcctg gccgaggacg gccgctttac
    10981 cgcgtacggt ccctgtcgcg gcggccgtac cggcggccgc tagatgtgcg gcatcttcgg
    11041 agtcgtttcc ctcaccgggc gtccgcttcg ccaccccgag tgcctcgagc agatggcgtc
    11101 gtccctggcg caccgggggc ccgacgggca gcggatcgtc gggcacgaac gcgtgcgact
    11161 gggcgcgcgc cggctcgcca tcatggacct caccaccggc gaccagccct ttcagagtcc
    11221 cgatggcgcg atctggatgg tgtgcaacgg cgagatctac aacgcacctg agctgaggcg
    11281 tgaagcggcg ggctggggat atccgttccg gtcgaccggt gacatcgaga cgatcgtccc
    11341 gcattacgag cgctcggggg ccgacgtcgt gcgccgcctc gacggcatgt tcggcctcgc
    11401 gatctgggac gatgcccagc gccgcctgat cctggcgcgc gaccgggccg gagagaaacc
    11461 cctcttctgg accgtcgtgg acgacgagct ccggttcgca tccgaggtcc aggcactcct
    11521 cgtcttcccc gaccaggccc ggcgcatcga ccgaaacgcc gccgggctct acacggcgct
    11581 gggctacgtc cccgccccgc actcgatgat cgccggtatc tccaagctcc cgcccggtgg
    11641 cttgctggaa gcttccgctg gtggcgtaac ggtgcgctct tactggagcg cggcggaggc
    11701 ggctcacggg cctgaccgcc tggagtccgc gcatcagctg cgcgatgccc tggtcggcgc
    11761 cgttcaccgg gagctgatgt cggatgtgcc gatcggcgtg ttcacgagcg gtggactcga
    11821 ttcgtccttt ctcgcggcgg cggccgcgcg cgccatgccc ggccagcgga ttcacacgta
    11881 cgccgcacgc ttctcggctg agggctacga cgaaagcgat ttcgcgcagg ccgtgacgcg
    11941 cgacatccat tccattcacc acccggtgac cgcggacgac gtatccctgg cgcgggcctt
    12001 cgaagtggtg acctcctgtc tcgctgagcc ggtcggcgac cccgccatcc tgccgaccta
    12061 cctgctggcc gaggccgcgc gggccgacgt gaaggtggtg ctgtccggcg agggagccga
    12121 cgagctgttc gggggttatc ccacacatct ggggcataag gccgctgggg tctacaggca
    12181 catccccggc ccgttccgga gtctgctgac caaggctgtg gagcggtggc ccacctctcc
    12241 tggaaaagtc accatcgagt tcctgctcaa gcagttcgta ggatccgcga gccggccgtt
    12301 attggagcga cacgctgcct ggttcggcgc gctgggcccc accgaagaca gcgtggacgc
    12361 gttcgcccgg ttgttgcggg acttcccggc ggctcccgat ctggaccgcg tgctctggct
    12421 cgacttcctc acctacctgc cggacaacct gctggtgaag gtggatcggg gcacgatgct
    12481 cgcttcgatc gaggcgcgcg cgcccttcct ggatcgcgaa gtcctcgagc tggtcctgcc
    12541 ggtgcccgcg gcccgcaagg tgcgggggtg ggagaccaag gcgatattga aggaagcggc
    12601 gtgggggttg gtgcccgcgt acgtcatcga tcgccggaag cgcggcctct cggtccccgt
    12661 ggcttcctgg ctcaatggtg ccctgcgaga ccgggcggcg cggtacttca cggcgccgcg
    12721 attcggcggg gagcgcgtga cggcgctgtg ggcggagcac cgcgaaggaa aagccaatca
    12781 cgcccgcaag ctttggccga tgctgatggc cgagatgtgg gccgaccgct ggaaagtgac
    12841 gctatgaccc gcgaagaagc gcttgccttg atgcaggagt acaccgcgtc cccctcgctc
    12901 cggcagcaca tgctggcggt cgaaggggcc atgcgtgcct atgcggtgaa atcgtcggaa
    12961 gatgtcgagg cttgggggct cgcgggcctg ctccacgact tcgactacga gcgttatccg
    13021 aacgacgcac actcggccac cgaggagcat cccagctggg gggtgcgcct gctccgcgag
    13081 cgtggcgtcg gggagcccgt gtgtcaggcc attctggggc acgccgatta tacgggtgtg
    13141 ccccgggaga ccgccctcgc gaagacactc tttgcggtcg acgagctctg cggctttctg
    13201 gtcgcgtgtg cgctggtccg tccctcgaag agcctcaccg acctcgaggt ctcgtccgtc
    13261 aagaagaagc tgaaggacaa ggcgttcgcc cgcggcgtga accgtgacga cgtccgccgg
    13321 ggggccgagg agctcggggt accgttggac gaccatatcg cgttcgtcat cggcgccctc
    13381 cgccccatcg agcgcacgct cggcctcggg ccggtgtaga cgccgtgcat ccgggcgcgg
    13441 acgggctcgc gcgcggcctc gaccgcgccg ccggcacccg gccgattccc gggaatctga
    13501 tcacccacca cgcggcgtcg gctcgcgccc tatcggccat gctcgcgatg atcggggaag
    13561 cccggcgctg ggtccatctc gagaactaca tcatccgcga cgacgtcacc ggccggcgct
    13621 tcgcggacgc cctgtccgag cgcgcccgcg agggtgtgcg cgtgcgcgtg ctgtacgacg
    13681 ccttggggag ccggaaaacg ggacgccgct actggcagaa cctccggcac gccggcgcgg
    13741 aggtgcgtcc cttcaatcca ctcttcgacg gacgtccctt cggttggttc cgccgcgacc
    13801 accggaagct cctcgtggtc gacggcaacc gcgccatggt ggggggactc tgtatcggcg
    13861 acgagtgggc cggcgatccg gcgcgtggcc ggcagccctg gcgggacacg gcgctggcgc
    13921 tccgcggacc ggccgccgcc gccctcgaca gcaccttcgc ccgcgtgtgg cgccgcgccg
    13981 ggaccgccat ccccgaggga gagctcgcct cggaccccga ggagtgcggg ccgaccgcgg
    14041 tccgcatcgt cgagggcgtg ccggaagggg cgcggatctg gcgggtggtc tcgctcctcg
    14101 cgacgctgtc cacggagcgg ctctggatca ccgacgcgta cctcgtgcct ccggcaccga
    14161 tcgtcgccgg attggtggac gccgcgcacg ccggagtgga cgtgcggatc ctggtgccgg
    14221 gagcgaccga cattcctctg gtgcaggcgg cgacgcggat cgggtaccgc gatctgctgc
    14281 gcgccggcat ccgtctgttc gagtggcggg ggccgatgct tcacgccaag acgctcgtcg
    14341 ccgaccggcg ctgggtccgc gtgggctcga gcaacctcaa cgtctcgagc ctgctcgcga
    14401 actacgagct cgatgtcgtc gtggacgacg agtcgctcgc gacggagatg gcggagcaac
    14461 atcgccgcga tgccgcggcg agcagcgaag tcgtactggt accgcggcgt cggctgccgc
    14521 cccgcctgac caacgcgcag gcggaagccc cgacggtcgc acatcagccc acggcgcgcg
    14581 agcgggccaa gacggcggta gtcgccttgc ggcaggtggc gggcggattg cgccggcgtc
    14641 tggccgtggc ggccagcgga ggcgccgcgt tgcttggtgc actgttgctg ggattcccgc
    14701 acgtgacgag catcacgttg gcggccggag cgttcgcggc cgccttcaca ttcggctggt
    14761 acgccctggt ccggcgggcg cgcgagcggg gacaggatgt cccctgattc ccaaccccgg
    14821 gccgggggtg cgcgtataga acaccgggag gaagccgagc tcgtcacccg gtcgctggcc
    14881 ggcgatcgcg cggcgttcgg cgctctggtg gacaagtatg cggcgcaggc ccggcgggtc
    14941 gcgcgggcgg ttttgcggga ccccgacgat gccgacgacg ccgcacagga cgccttcctt
    15001 tcggcgctgg tcaagctgga gcagtacgat ccgcgccggc cgttcggtcc ctggttgatg
    15061 cggatcgtgg ccaatgccgc cacggatcgg cgccgtcggc gctcggtacg cagtgccgag
    15121 cccctcgacc cgggcgtggt cggagggggc cctcggcccg ataccgcggc ggagcgcagc
    15181 gcgctttcgg aacgcctccg ccaggcactg gccgagctgc ccgagcgacg gcggctggcc
    15241 gtcgtgctgt tcgatgtcga ggggtattcc cacgcggaga tcgcggggat gttgaagatc
    15301 cccgaaggga cagtgcggtc cgaagtgttt cacgcgcgac gcaagctgcg cacgttactg
    15361 gaagactgga gggagccgtc atgatggaag agctgccgtt cgatcacgag caggatgccc
    15421 gcctgggcgc cgcgctccgg gaggccctcg cgcctgccgg ccatgaggcc ttcgtcgcgc
    15481 gggtgatggc gggtgtctcc accaccaaag ccgggtcgtg ggacgtgctt gccggctggg
    15541 ccggccgcgg catcgcggcc gcgctcctcg tcgccctcgg cgccggattc gccgcgggtc
    15601 gcgcgatgca gccgtcgaac ccctcgatcg aggaggggct ggtcagcgct ccggcagact
    15661 ccgggaccgc cgcggcggcc ctggtccgcg ggaacaatcc accggacgcg agcatcgtgt
    15721 tcgcggtgcg gccgtaaccc gatgttcaac cgttcgaagg cacaggcact cgcgctgctc
    15781 accgcggtgt tcgtggtggg tggcgccgcg ggatgggagc tcgatgcctg ggcccaccgt
    15841 ccccgtcccc acggtcccga ggcgttcgtg aactacctga ccggagagct gaagctcgac
    15901 tctctgcaac gggattcggt ccgggccgta ttggtccggc acaagccgca gatggacagc
    15961 atctggaagg acgtacacac gcgcgtggat tcgctccgcc acgtcatgcg gggggaaatc
    16021 ttcacccacc tgaccgacgc ccagcggacg aagtacttgc aactggtcgc ggagcaagcg
    16081 catcaaaggg gtgactccac ccgcgacacg actcatggag gcaggaacta gaatgcggtt
    16141 gagcatcttg atcaccggtt tggtcgtcag tggcgggctc gcatcggcgc agacgccgtc
    16201 gcaggccccg gacagtgggc ccccgaccgt taccctcacc gacgccgttc aacgggcgct
    16261 cacggttcag ccggcgatgg tgtcggcgat cggcagtcaa cgcaacgcca gcgcgtccag
    16321 gacctcggcg tttggcactt ttctcccttc cgtcaccgcg ggctggtccg cgtccaagag
    16381 cagccagtcg cgattcgaca acaatcgaag cgtgttgatc ccaccgacgt ggaactacgg
    16441 gttcaatatc aacgcgtcgg tcgatctgtt cgatggcctg cgccggatct ggaacgtgaa
    16501 gtccgccacc gccgtgcagg atgccgcgga cgccggttac atcagccagc gcttcgtcac
    16561 cgagctgcag accaaacagg cgttcttcgc ggcgctcgcg gatcaggagc tgcttcgagt
    16621 agcgcaggcg cagcttcaga ggacggagca ggaactcaaa gccgcgaccg acaagctcag
    16681 attcggggcg gctactcgcg cggactccct gacggcggcc gtggatcatg ggaatgcgga
    16741 agtcgcgttg ctgcaggccc aggccaacct ggtcggcgcg caggcgaacc tggcccgaca
    16801 aatcggcgtg aatgggctgg ttcagccggt agccgattcg accctgccgc ccctgcccga
    16861 caccacgggc ttcctccggt cggtgatcga gaacgcgccg ctcgtgcagc aggctgatgc
    16921 ccaagccacc gcggcccgtg ccaacgtcct gtccgcccgc tcacagtacc tgcccacgtt
    16981 caacgtctcg ttcggcgaca atcggagcga cacgacgttc ggcggcggct tccttcatgg
    17041 tcacgtgacg cggtggacgt tcggcgcctc tttcccgctg ttcaatggct tcgtccgtga
    17101 gcagaaccag gtctcggcga gcgtgaaccg ggatgttgcc acggcgaccg cagccgacac
    17161 ccggcggaac gtcgcggccc agctcgcgca gcaagtcgcg gccttggtgg cggcctatca
    17221 ccagatcgag ttcgcgcgat cgaaccgggc ctccgccgcg gaggcgcttc gcgttcagca
    17281 gcagcgctat gcggcgggcg cggggctcct tctcgacctc ctgaccgccg aggcgaatct
    17341 gactcaggcg gaagcgacgc tgattcaggc gcggttcacc taccggacta cgcgcgccac
    17401 catcgaaagc cttgtgggga aggaactgtg atcgccaccg catctcctat gcaggaattc
    17461 cggaccggag atacgccgct ccccgatgtc gtcatcctga cgcaccggct gacgcgggag
    17521 tacgtcatcg gctcggagac cgtccgcgcg ctgcgcggcg tggatctcca gatccggaag
    17581 aacgagttcg tggcgatcat ggggccttcg ggatcgggca agtcgacgtt catgaacatg
    17641 atcggctgtc tcgatacgcc gaccgacggc gagtattggc tgaacgggca gaaggtcagt
    17701 gagctccagg acgactccct ggcccgcatc cgcaacaagg aaatcgggtt cgtgttccag
    17761 acgttcaatc tcctgccccg cgccaacgcc ctccacaacg tggagctgcc gttgatctac
    17821 gcgggcgaga cgtcgagggc ccgcaaggaa agggccgcgg cggccctcgc gaaagtcggg
    17881 ctcggcgacc gcatggacca ccgtcccaac gagctctcgg gcgggcagcg ccagcgcgtc
    17941 gccatcgcgc gcgcgctggt gaacgatccc agcattctcc tggcggacga gccgacgggc
    18001 aatctagact cggccaccgg caacgacatc atgcggttgt tcgaagaatt gtgggaagcg
    18061 ggacagacca ttgtgctggt gacgcacgaa cccgacatcg cggcccgcgc gcaccggcag
    18121 atccacctgc tcgacggcaa gatcgagcgc gacgagagga cggaatcgaa gtgaagcgca
    18181 tcgttttggt gggcggcctc gccgccctgg gcttggccgg ctgcaagaaa gccgcgcagt
    18241 cccccgtgta catcgcggtc ccggtggaac ggcggaacat cgtcgtggcc gccgccgccg
    18301 cgggacccat ccagccgatc ctgacggtga gcgtgaagtc ccaggcgtcc ggcgccgtca
    18361 ccgaaatgca cgtccagacc ggtgacgatg tgaagcccgg ccagctgctc gcgctgatcg
    18421 acccccggct ccccaagagc gcgctcgatc aggcgcaggc gacagtagac caggcccagg
    18481 cgagcttcga ttacgccaag gcccagctgg caagggaaga cacactctac aaggtgcagg
    18541 cagtcacgca gcaggagtac caaagcacca aggtgtccta tgagcaggcc cgcgccgcgc
    18601 tcatcaacgc gcaggcgaac ctgtcggacg ccaagatcgc gtacgaccag acgcgggtca
    18661 ccgccaccct ggccggcacc atcatcctga aggaagtgga catcgggacg gtgatctcga
    18721 gcccgaccca cgacgtcacg ggcggcaccg agatgttcca gatggccaat ctggacacgg
    18781 tccaggttca ggcgatggtc gacgaaaccg acgtgggcaa ggtacaggct ggccagccgg
    18841 tgaccatcac cgtcgacgcg tacccgagcc ggcccttcca ggggaacgtg ctcaaggtgg
    18901 aaccgaaggc caccgtcagc cagaacgtca cgcagtttcc ggtcgatatc gacattctca
    18961 atccgggtca cttgctgaag ccgggtatga acacggaagt cgagattcac gtgggccagc
    19021 gcgattcggt gctggcgatc ccgacctcgg cgctccggac gcagaaggac gtggcctcgg
    19081 cggcctcggt gctcggaatg gatcccaacg tcgtgatgca gcagctcgcg gcggcgaccc
    19141 cgaagccggc gccgaacacc aagggtgaca cggcggcctc gatgggaaac agcgccggcg
    19201 ccggcaaagg caccatgacg atgcccgacg ggcgcgtggt gaagctcccg cagggtgtga
    19261 ccgcggcgca ggtacagggg atcttcgaca agatgcgaag cggcgggttc agctcgatca
    19321 cggcggcaga ccgggacgtg atgaccaagt tgcggcaggc caaccccgac atgatgggtg
    19381 gcggtcgcgg ccgcgggggc cgcggcggcc cgggcgggcc ggtcggcgga ccggtcggga
    19441 acggcacgca gtccaacagc tacatcgtct tcgccctgag aaagggccaa ccgaccgcgg
    19501 tgtcgatcaa gacgggactc tcggatctgg actacgtcga gatcacgagc ggcctgacgg
    19561 aaggtgacac ggtcctgctg ctgcccagcg cgtcgctggt ccagtcgcag gcgcagttca
    19621 agcagcggat gcagggcatc accggtggcg gcctgccggg cgtccgccag acaccgcagg
    19681 cgtcgggagc gcggtaacca tgctgctcgg cgagatcatc aaggtcgccc tgcaggcgat
    19741 ccgggcgaac aagctccggg ccctgctcac gatgctcggc atcatcatcg gcgtcggagc
    19801 ggtgatcacg atggtcgccc tcgggaccgg cgcccagaaa tccgtgcagg accggatcca
    19861 ggccctgggc ccgacgctgc tcacggtgtt ccccggccag agtttccggg gcggcgtggc
    19921 cttcagcacg cgggtcagcc tcacgatgga cgacgacacc gcgctgctca acaaccggca
    19981 ctacatcagc gaagtggtgc cggagctgca ggggaacctc caggtccagt acgccaatca
    20041 gaacatcaac accaccgtcc tgggcagcac cgcgaactac tactcggttc acaattacaa
    20101 catgatcgcc gggcggatgt tcacgaacgg cgaagatgac gcgcgccagc ggtacgcggt
    20161 gctcggcggt caggtgccgg aggaactcaa cgccaacccg caggcgatga tcggacagca
    20221 actgatgatc cggggcattc ccttcgagat catcggcgtc ctgtcgaaca agggctccca
    20281 gggattcagc aacccggacg aacagattct catcccgctg cagaccgccc gctaccggat
    20341 cttcggatcg aaccggatcc gtgaccttac ggtcgaagtg ggcgatctcg cgaacatgaa
    20401 tctcgggatg atcgagatcg agtcgatcct gcggcgccag cacaagatca ggcccgggat
    20461 ggacaacgat ttccagatcc ggaaccagac cgacatcctc agcacgttcc aggagacggc
    20521 gcaaacgttc gggttcttgc tcgctggaat tgcgactgtg agtctcatcg tgggcggcat
    20581 cggcatcatg aacatcatgc tggtgtcggt caccgagcgc acgcgggaga tcggcgtccg
    20641 gaaggcgctg ggcgcgaccc ggttcaatat tctcttccag ttcctggtcg aggctctcgt
    20701 gctctgcatg gccggtggca tcatcggcat catcctgggc tcagggggag cgatcgtcct
    20761 ggcgcagttt gctcactgga atacgtcgat ctccttgtac gcgatcgtcc tggcgttctt
    20821 cttcagcgcc atcgtcggtc tcctctttgg cgtgtggccg gcgcgccgcg ccgccagcct
    20881 cgaccccatc gaagcgttga gatacgaata gcgcgggacg aacaggggaa aacgcaggac
    20941 ggaaagcggg aaacgtgatt ggtcacgttt cccgtttctc gtttcgcgtt tcccgattac
    21001 tttccgggcg tgctattctc tctcttgctc gcactgcaag gtgcccagac gccggatccc
    21061 caccccgtgt tcgcaggccg gagtggcgcg ctcaacgtcg cgatcccgcg cgtcgacgcg
    21121 aaggtcaccg tcgacggcaa cctggatgag ccggtgtgga atcaggccgc gctgctcacg
    21181 ggcttctcgc agtacacacc cgtcgacggc ctgccggccg cggactcgac gcaggtgctc
    21241 gtatggtacg gatcggacgc gatctacttc ggtatccggg cctacgaacc gcatggcgat
    21301 ctggtccggg cgacgatggc cgaccgcgac gcgatcgacg cggacgacaa catccagatc
    21361 ctcctcgaca cgtacaacga tcaccggcgc gcgctcgaat tcgcggtcaa tcccttgggt
    21421 gtccaggaag acggcgtgcg cagcgaaggg ctcgcgggtg cggcgggagg cggcaacgcc
    21481 gccaccggac gcttcgacgg ggtgatcgac ctgaaccccg atttcgtctg ggagtcgcgc
    21541 gggcacgtga cgccgtgggg gtaccaggtc gaagtccgca tcccgttcaa gagcatccgg
    21601 taccagagcg tcgatccaca gtcctggggc ctgcaggtca tccggtacgt gacgcactcg
    21661 gggtatcagg acacctggac gcccgtggtc cgcgccaacg ccagcttcct cattcaggcg
    21721 gggaccctga cgggcctcag tgacctccac cggggcctcg tgatggacgt cactccggaa
    21781 ttcacgagca aagtggaagg ggactcggcg ggtgcggggc gctacctcta caagggcaca
    21841 ccggaaatcg gtggcacgct gcactggggc ctgacgagca acctgagcct cgccgccacc
    21901 gcacaccccg atttctcgca ggtcgaagcc gacgccgcac aggtgacggt gaaccagcgc
    21961 ttcgcgctct tctatcctga gaagcgcccc ttcttcctgg acggcctgga gcagtacgac
    22021 acccccaatc agctcattta cacgcgccag atcattcaac ccgtcgcggg cgcgaagctg
    22081 accggcaagg ccggtggtac caacatcgcg tacctcggcg ccgtcgacgc cgtcgacccg
    22141 gccacgggga agaacccggt gtacgacgtg atgcggatct cgacggactt gggggcgagc
    22201 tccacggcgg gattcgcgta caccgaccgg gtcgagggaa ccgattacaa ccgcgtcttc
    22261 ggagcggact cccgcgtgct ctggggcacg ggctggttct cgcaggcaca gatcgccggc
    22321 tcctggacgc gggatgcgtc gggcgtgcgt tcgggcacgc tgtgggacgc caccctgggc
    22381 gactatacgg gccggcggta cggcaatcat ttcgagttcc tggggagcac gccgaacttc
    22441 cgggcctcct cgggcttcgt gaatcgcacc gggatcgtgt ccgcccgcct cttcaatcgc
    22501 ttcacctggt acgggaaacc cggcgcgctg atggagcagc tgcagacgat cgtgatgggg
    22561 acgcccctct ggcgctacga cgacttctgg catgcacgcg ggaccatcga gggaggctgg
    22621 gcaaacagct gggggctcac gttgcggggc gggtggaacg tgagcgcggc agtgaatgac
    22681 aatctccagc gcttcgattc cgccacctac gccgggtacc aggtgaatcg cgggatcgac
    22741 acgatcccgt tcaccatccc ccacgggctc tacaacctgc tctcggccaa cgccggcatc
    22801 accagtccct accgggcctt tcaatggtcg gtgagcgcgg gctacggcca ctccgtgatc
    22861 ttcgcggagg ccgcggaagg caaagagctg acgacttcag cgtcgatcgc ctggaagccg
    22921 acttccgcca tccgggtctc cgccacctgg ctgcatgacc ggatcgttcg ggagcgggat
    22981 ggcagcgagt tcgcgctggc caacattccg cggctcgagt tcgactacca gctcagccgg
    23041 tccgtcttcg tgcggtacat cggtcagtac gtcgctcagc gccgctcggc cctccaggat
    23101 ccgcgcaccg ggcagccact cctggtcgac agcaatgccg tcgtcgggag cctgtccaac
    23161 ggattccgga acgacttcct gctctcctac aagccgatac cggggacggt gttcttcctg
    23221 ggatatggga cgtcgctctc ggagccgacg gcgttcggat tctcgaactt gcagcgggtg
    23281 caggacgggt tcttcctgaa ggcgagctac ctcttccgca tgtaggcccc ctttacgtcg
    23341 ccaccctcgg cggggcggcg gtgctgtccg aatcgatgtt gaagtacgcg tcgatctcgt
    23401 ccagcagatc gtcgtccgag tagatcccgg tgaggatccg ctccggaccg ccgggatcga
    23461 gcaggacgta cccgtacgga tagtcgagcc ccgacaacac gatcatgcgg cgacggtgac
    23521 cgggtccctc cacgtagacc gtgtcggccg tggtgggaac gatcgcgacg ccggcctgcc
    23581 tgagctcggc ggccacctga tcggtatagt atgtcaggtc atcgtacgcg gagctcgcgt
    23641 ccccggcgga caatgtgtcg gcggcgccga gccagaagac gatcatggtg ggcgcggtga
    23701 tgacgcgggg gcggcggagc accgagcggc tggaatcgcg gagctcgagg gagtcgcgtc
    23761 cgtggacgcc aggcagtctc tgaagaccgc ggcgattgtg cgccaacggg gcgtgcggac
    23821 ggcacccgaa ggcgaaaaga ccgagcagca aagcggtggc cgacttcata tacaggaata
    23881 tattcgagcc atgaccacct ccgcgcccgc cccagtccgg cgccatcact ggatcgtccg
    23941 gctgtgccat tgggccactg tcttcgttct ggcggggctg gtcgccagcg gtctgcagat
    24001 ctacgaggcg tacagccgct ttgggaaccg gggcgaccct ggttatccca gcccattcaa
    24061 cggcacggtg tttccgcact ggagccggct gggaggctgg ctggcggcgg gactgaactg
    24121 gcatttcgcc ctcatgtggc ccttcgccct cggaggcctg ctctatctct cctacctcgc
    24181 gttcagcggt gaatggcgat ccctgctgtt ccgtccgcgc gatatccgcg gcgccatcga
    24241 gatgcagaag tactacctgg gaattcgaaa agaccatccg gcacagggca agcacaaccc
    24301 cctgcaaaag ctggcctaca cgttcatcat cctgctcggc acactgtcgg tgctcaccgg
    24361 tctgtcgatc tacaagacga cgcagttcag ctggctcacg agtcttttcg ggggcttcca
    24421 ggcggcgcga tactggcatt tctgggccgt ctggatcttc gtcggcttca cgctgacgca
    24481 cgtcgcgctc gtctttctcg tcgatccaca ttcccttcgc gcgatgatca ccggctggta
    24541 ccgcgggagg tttccgagcc atgactgacc gccgctcctt cttcaagacg gccggcatca
    24601 gcgccgcctc cttgctcgcc ggctgcggct gggacggcgg gaagagcgtc aagcctgccc
    24661 tgctctccat ctcccgtctc aacgactggg tgggggaaca cctgctctac tcgccgagcc
    24721 ggctcgcgaa gaactatgac ccgagccgcc ggagcgtcgg cctgccggcc tatttcatct
    24781 ccgataccgt gccgatgctg caggatcccg cggcgtggcg gctgtcggtt ggcggattgg
    24841 tgcggacgcc gctgaatctc acgctcgacg acatcaagcg catgcccagc gtgacgtaca
    24901 ctgtgaagca ccactgcgtg gaagggtgga gcgcgattgc gacctggacg ggagtcccgg
    24961 tgtccgccat cgcccggcag tccggcatga cctcctcggc tcactacatc gattttcagt
    25021 cgttcgactc gggctactcc aatgggtggg atctcgccag cgcgatgcac ccccagacca
    25081 tcctggcgta cgcgatgaac gaccgactgc tcggaccgga tcatggcgcg ccgttgcgac
    25141 tgtactcgcc caccaagctc ggctacaaga tgacgaagta cctcacggcc atgaacttca
    25201 ctgaacaaag gccgggcggg tactgggagg atcagggata tccctggttt ggaggactct
    25261 aggactcagg cagacgccta gtcgctcagg cgctcagcgg gttgaagtga gaaggggtca
    25321 ggagaatctc ctgactcctt tttcctgccc accgctaggc gcctgggcga ctgggcgcct
    25381 gtctgagacc catccgtcta gccgattaga tcaagggcgt cgtacatcga tgctgcagat
    25441 tggtatcggt ctccaggttt gcgtcccatg gacttgagga tcaccgccgc gagcgtctcc
    25501 ggaacttccg ggttgagcgt tctcgggtcc ggggcgttct cttccagatg cttcgcgatg
    25561 agcgcccagg gggtgtcggc ctcgaagggc acccgtccac tgacgcactc gaagagcacg
    25621 acgcccgcgg agtagagatc actgcgcgca tcgaggtcga gtccctgcag ttgctcgggg
    25681 gacatgtaat cgggcgtacc gatggacatc ccggcttcgg tcaatccctt gcccttgggc
    25741 agattggcga gccgcgcgat gccgaaatcc atgaccttca agaagccgct cggctccacg
    25801 accatgttct gcggcttgat atcgcggtga atcacgccct gctcgtgcgc cacttcgagg
    25861 gcccggcaga gctgcttgcc gatggtcagg gtcacgggaa tgggcagccg tcccctgccc
    25921 tcgatgaggg acttgagtga cttcccttcc acgaattcca tcgtgaggta gtacgtcccc
    25981 ttcacctccc cgaggtcgta cgtccgcacc acgttccggt gcgagatctt ccgcgccaga
    26041 cggatttcct gcttgaaacg gtcgagtgcc acggcatcgg cggacatcgc ctcgggacga
    26101 agggtcttga tggccaccgg ttcctgcagt tcccgatcga aggcccgata gacgacgccc
    26161 atgccaccca tccccagcac ttccttcacc tcgtaccggc cggcgaagag cgcgcccggt
    26221 gagagcatcg tgccggagct cgccgccgcc gcggcgcgca cggcggtcgg aacggccgag
    26281 atcggttgcg tggcggcggc gccagtggcc gacatcatgt actcgaccag atcactcttt
    26341 tccttcagat cctcgacgag atctctgaag tcccgcgaga gcatcccgat ctcgtcgttg
    26401 gacgacgtcg cgacgtccac cgagtagtcc ccgtcgcgga ccttccgcgt cgcctcggcc
    26461 agccgccgga ccggtttcgc gatggacgac gcgcctatgg aggcgaccac gagcgcgagc
    26521 acgaagccga ccccaaaggc gtagtagagc gcccgcttga gcgtgcggaa ggtcgccatc
    26581 tcctggtcac gcgaacggag cgcgacccac ccgccgaggc gccggccccc cgcgttcagg
    26641 atcttctctt ccagaccgat gtagtgactg ccggacatgt cggccgccat ctccatgccc
    26701 gtggtgtcgg ccgaggtgtc ggccccgatg ttggccgcca ggaccgggag cagctcggcc
    26761 gctggaaccg tcgctcccac gggatagggg cgccccaggg tatccacaga aaagaagaca
    26821 aggtcggttt gggtcgcttc cttgattgcc gccgcgagag cgtcatcgaa aggatatgcg
    26881 accacgagta cgccctgggg cgcgtcttgc gggctggccc gcagggggat cgcgacggcg
    26941 tagtacaggc tgtgcgtttg gaggtctcca aacgcgccgg tggtctggtc gccggagagc
    27001 gcgttcgaga acagcacgct gttggaataa tcgatgctgt ccgcggccgg ctggtcggtt
    27061 cgggcgatca gcaggccgtc ggagttggtc accatggccc aggcggctcc agccaagttg
    27121 cggatctcat tcgcctgatc gagcacgtcg gcgcgttttt gtccctgcag cagccgagtg
    27181 cgatactgcg gaacgcttgc cgccgcggtc gcggcagccg ccatggcccg ggtgcgcgcc
    27241 tcgagctgcg cgtccacggc acggtgcgta ttctggagcg ctttgcggat ggagacgtcg
    27301 gccgcgcggt tcgcctgcca ggatgtgtag accagcgtgg cgcccagcac cgccacgatc
    27361 agaacgaccg aggcggcgaa gaccttccag gccaggccga aacgcggccc cttgtcagta
    27421 gagctcgcgt cccgcattcg tcggataggg tttcccgtac ttgttcttgt gggcccgctg
    27481 cctgaagccg cgcgcgtcga gctgcaccgg ctgcgccagc gtcagtcccg ccgcggggac
    27541 ctccacatcc tgcacgactt cctgggatac gcgcgggtgc caggcgtgca gggtgtagcg
    27601 gccggcgggt acgccactga tggagaagct gccgtcggcc cccggctggg tgaaccagcg
    27661 gctcgccatg acctgcacgt aggccaccat gctcgagtga acattgcagt agatgtagac
    27721 caggccggca tggtcgaaca cgtgtccctt cgcctcaccc cggccataga gccccaggtc
    27781 aaagccgttg ggatcggacg ccgagaaaac attgtggctg aagggatcgg tattcgggaa
    27841 acggaccgtc gagccgaccg gcaccacgat gacgtcggga acgaacttct tgtcggaaat
    27901 ggtcacgtcc agggcgaccg gacggccggc ggattcattc ccgccggtga ggtagatcac
    27961 ggcatccccg atgtcggggg atggcttatt gtgcttctcg aggatggcga tcgtgcccga
    28021 cacgcgggag gcgggaacag tctgcgacag ggcccccggg gcggcgagca cccccatgac
    28081 gaaggtcaac ctcaagtgcc gcatgcgcgt aggcctctcc gagtattgcg ccggtagccg
    28141 cacagcttag tctagggacg ccgaaaccgc caggagacac acgtgaaacg catccttgcc
    28201 gttctcacca ttctcaccgc tccggctgcc gctcagcagt cgaagcccgc gcccccgcag
    28261 cggacggtgc aactgacggg catggtcctc gtgaacggat tctggaacag ctcccaggtg
    28321 tccaacgtgg acctccccct cgcggcggtc cctgactccg tcggcgtcaa agggggcggc
    28381 gcatccatcc ggcagacccg gattggcgtg ctggtgaacg atcccgacgt gctcggcggc
    28441 acgttcaccg gggagatcga cgcggatttc tacggcggac agcaaccgag cgggagcggg
    28501 agaacgtcac cgctgttgcg gttgcggcgg gcgaccggtg aaatcgactg gtcgcacgtg
    28561 cagctgatgg tcggacagga aaagccgctc gtgtcgccgc gaaatccgcg ctcgctcgcg
    28621 gcagtgggct tccccgagtt ctcggggtcg ggcaacctct ggctctggat cccccagatg
    28681 cgggtgacgg tcgaaacggg atattccttg cggctcgcgc tgcaggcagc cgtcatcgcg
    28741 ccggccgctg ggaccgctca gcccgcgttc aacaccagtc tcgattccgc cgaacgcacc
    28801 ggccgtccct atctcgaagg gcgcctccgc ttcggatggg gtgccgcgga caacccgagc
    28861 gagatcggca tcggcgttca tcagggttgg ctcaagaatc tggatagtct ctccggggac
    28921 tccatccagg tgtcgcaggc ggtcaccgcg gatgcgcggt tcctgttcgg cccattcgag
    28981 ttgatcggcg aatttttcac cggcaaggga ctcgccgggc tcggcggcgg tggcgtcggc
    29041 cagaacgtcg gtgtcgacgg cgccgtcgtg catacgacgg gcggatgggg ccagttcaac
    29101 gcccgtccca tgaaggactg gatgttcggc gttggctgcg ggatcgacga cccgaaggat
    29161 gcggacgtct acacggtggg agactcgacc tatctcacca agggtggccg cctcaagaac
    29221 cttgcgtgcg agggtcacct gcagtggacc ccgcccggtc ccgtcgtgct cggcttcgaa
    29281 ttccggcaga tccggacgac ctatctgacc ggtgaagagc gggtcaatca tctgaatctg
    29341 gcgatcggct accggttcta gatcaggcca ccggcagcct tccaaccaag gttgccggcg
    29401 tgaaagggcc cggctcggcg atcagcgcgc gggcatgttc gacctggccc cacgtattcc
    29461 agagcagcac gcccttcacc ctgccctcgc cgaggaagta gacgacgccc tcgcggaagg
    29521 gtgccttcca gtcgctgacc gtttcgaggc gcgcatccag gtcccccacc gcctcatatc
    29581 ccagctcgaa cagatccccg
//
LOCUS       DASUBP010000024        11834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1322578, whole genome shotgun sequence.
ACCESSION   DASUBP010000024 DASUBP010000000
VERSION     DASUBP010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 11834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11834
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1322578"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="VFU75_01065"
     CDS             complement(<1..817)
                     /locus_tag="VFU75_01065"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HEU5465448.1"
                     /translation="MNRHLAGLLAGSLVAWADARHRDGTGRGPRYLLARGLSPLVRSL
                     LRPDLARVPFPVHLRRRLELLGPTYIKLGQILSLREDLLPKAVTDELKHLLGRLPAIP
                     FADFQTLVSRELGRPLDDLFLRVDPEPLGSASIAQIHRATTRAGDPVVIKAVKPGIVE
                     TLRRDALLLRILGAVLEWPFGRYQPRRIIREFVAYTLREVDLKQEAENARAFAANFAD
                     LAGVAFPVVYPEYSGARVLCMEFVDGLRPDTAAARALPARARRQLVELGAESII"
     gene            complement(946..2127)
                     /locus_tag="VFU75_01070"
     CDS             complement(946..2127)
                     /locus_tag="VFU75_01070"
                     /inference="COORDINATES: protein motif:HMM:NF012588.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HEU5465449.1"
                     /translation="MPTIRSGIPSSRHGTDDYDESLIRCRREYVEARTGATLHHLSQY
                     SFDPLETRGNIENFTGVAQVPVGFAGPLHINGEYAQGEFLIPLATTEGTMVASYNRGM
                     KVLNLSGGVLTTVSADGMQRAPVFVFDTAREARAFELWVNEHLDDLRREAEATSHVAR
                     LRYIDVYLSNKFAYLRFNFVTGDAAGQNMVGRATFAACQWILEHADGIRRFYLESNLA
                     TDKKYSQVNVMRTRGKRVTAEATISREVLATHLRVKPEVLQHHWGVGNVGAFLSGANN
                     NGLHSANAITALFIATGQDVANVSEGSAAIIYSEVTDAGDFYLSITIPSLIVATHGGG
                     TGLPTQRECLAVLGCTDAGSVNKLAEIVAGVVLAGEISLAGAISSLEWVSAHERYGRK
                     R"
     gene            2242..4239
                     /locus_tag="VFU75_01075"
     CDS             2242..4239
                     /locus_tag="VFU75_01075"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HEU5465450.1"
                     /translation="MPQVLSSRQFATLAAVCESLLPSIQVDDDPEGLFALGAVQAGTA
                     GRAADLVGALRDPGDARRLRFLLTALDTPWAGLFLAGKFAGFRALPPAARERALSRLA
                     FSRLPPLRAGFQALKRLGHVAHYCWPVVNGGAPTHPAWRAVGYPGPLAPPAHPPLPVS
                     ARRVETDMVIDADVVVVGSGAGGGVAAGVLAEGGRHVVVLERGINPAPEDLTQIEGDM
                     LGRLYLDGGLMMTQSGSIPILAGGCLGGGTAINYTTSFPLPPETRAEWDEISGLRLFS
                     SPRFGDSLDRVMRQLRVGTTWSTPGERDQLLERGCRALGWHVGVIPRNVSGCCEGVEC
                     GYCGYGCRHGAKNSTARTYLADAARRGADIIVGAEAEQILIERRQVRGVAVRARGENG
                     AEHRLVVRAPVVVVACGSINTPALLLRSGLRGPRIGRGLRLHPASAVAGVFPYRVEPW
                     TGSLQTRYSDQFASIDQGYGVKFETAPVHFALPASGFGWESSRRHREDIGRLGHTSII
                     GLLLRDRDPGRVTIGPHGGPRVHYELSRFDEAHIRRAIVGGAQALAAAGATEIFSLHT
                     PPVRGRPGTPGWLDRFGREADSRGYRRCRMSYISFHQMATAAMGATPLLGAVDEAGES
                     FEVGGLYVADAAGFPTSSGVNPMITIMALADHVARGILETS"
     gene            complement(4259..5104)
                     /locus_tag="VFU75_01080"
     CDS             complement(4259..5104)
                     /locus_tag="VFU75_01080"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5465451.1"
                     /translation="MYRFSLAEGFEPSPTRRAVGITLVVLAMGAAAFAVGCGHKSTTG
                     TTTASLPTSTKATPTGPAVPVTPPGPTPPTVSHYKLAVTAWRGGDRDGAVTEFRAAIA
                     EDSTKLPPRLNLSRVLIEQGKAKDAIDEIQQVIAIDSTSGSAYRLLGRAHDFLGQTDS
                     AVADYQHAIVLNNQDGWSINNLGLVQIERGEYDEALKTLSRAVELSNSATFRNSLGVA
                     LEKTGHYTAAVEAYKSALSADSGYTRAQTNLTRVTSLTEAPGTPAVDLAAQALEFMRE
                     VETWK"
     gene            complement(5288..6496)
                     /locus_tag="VFU75_01085"
     CDS             complement(5288..6496)
                     /locus_tag="VFU75_01085"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5465452.1"
                     /translation="MITAQARAQTPVDPSLAAYIASIKAIDNHAHPMAVVPPGGAPDT
                     DYDALPLGGIPDFPLPWRLTLEAPAWGQAAHALAGVTTPAQALDRVGIQLEFANRIAM
                     GAGLDSGRFRWVPFDDPLIFPLDTRLVGQTPDTRALYPLEARLLRRYLGDLGLSALPP
                     TLAEFRSQVVVPLLQRQHGSGARAIKFEVAYLRSFDFAAPDTARAAAIYAQFAGGGTP
                     SAEEVKILSDALFRDVAREAGKLGMAVHLHALEFFGGYYEAEGARPGLLEPVFNDSTL
                     RRTNFVLIHGGWPHVGETEAMLAKPNVYADISMMDLILSPEELATVLRGWLMRWPDKV
                     LFGTDAFDGGPKQTWEMGAWVASNTARRALGMALTAMVRDGDITMAQARILAKKVLRD
                     NAIALYHLSR"
     gene            complement(6557..7891)
                     /locus_tag="VFU75_01090"
     CDS             complement(6557..7891)
                     /locus_tag="VFU75_01090"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5465453.1"
                     /translation="MTTVLVVDDVPAMAEQYAYDLRRLGKYQTLVAAGGQAAIDVIER
                     EAIDVMILDLEMPVLDGFAVLKSVGRRVPVIVYTGTGSYDRCLQAVQLGARSFLDKAE
                     PMERVVREVEHVLERERLTREVATLRASEGALVGTSAALTRVREQLNRLAPIPSAVLV
                     LGDSGTGKELVAREIHRVSARRGLLVAVNCAALPSELVESELFGHERGAFTGAHTMRK
                     GAFETAAGGTLFLDEVGELPLAAQAKLLRVLEQREITRVGGNRPIPVDARVVAATNRQ
                     LEQEVQAGRFRLDLYYRLCVHVVRVPALAERRSDIPALVEHLISVVGSRFGMRPRPIE
                     PDAVEVLARADWSRNNVRELKNVVERMLIAADSEVLGVGDIPREVREAPAAETGAMAG
                     TFRELRAQAERRILLTALDRNAWHITRTARELGLADHASLLKILRRHGIRRN"
     gene            complement(7888..9564)
                     /locus_tag="VFU75_01095"
     CDS             complement(7888..9564)
                     /locus_tag="VFU75_01095"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5465454.1"
                     /translation="MTFRARLAAAFLPAVLLPLVVFGWGVRRAVGSRLEAMFERRADA
                     ALDHVRARVAGTDADLARRLAGVRRAAGSDLHLRAALAADSGDDYVRDFAGTAMRVSG
                     LASLQILSPDGTVLSSGHFRNAYGWAESSLPAHLRATPDSIMLIRVRSAESTFVTLAR
                     LDSLRVGDREYPIVGGIGLDSEWMARSAPDADVSVALADSAVPAAHVMPLPFLSSEDA
                     DSVGTASLVVDQSLAPLTAISGSLNRWLALALFTTALTGLALALWLAGRLSRPLADLT
                     ARAEDLDPEHPEGAFDTPRLAPRDEVGKLAGVLSTMTARLRESAETARRAERRAALGD
                     LARQVNHDIRNGLTPIRNVFRHWSDAAGESPEELARVFAERQGTVESSITYLHALAGE
                     YARLAVPGVVEPCDLNVAVQGAAQRLAGDEGVTVQVELAEPAPVVRADPLALRRVIDN
                     LASNARESLVNGRGVVTLRTRGGEHVCLQVIDTGAGMSRAQLEHALSGFHSTKPQGSG
                     LGLTIVRRLVGDAGGALRVDTAPGAGSSFTVEFPRLETAGVAGEEKAGDR"
     gene            complement(9561..10796)
                     /locus_tag="VFU75_01100"
     CDS             complement(9561..10796)
                     /locus_tag="VFU75_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465455.1"
                     /translation="MSKTTIAQSFLATIAVAGIFACAHSVMRVSPPAANHFPANCVAA
                     GTPGAPRDSLRVVEDGPVESQDAPVPRSAAERIAFGLAYETLVKVDCQGNLLPGLAAS
                     WRTGTDGVEFVLRKDARFWDGAPVFAADVKSSWRLTDSTLAERTTVAGDTLIRISTGS
                     GDALAAVLAPSAAITKPAPDHGWPIGTGSRWLTNPATDADDAVLQPTTPGTAPRITFA
                     RGTGDARDALESHADVVITGDRAALAFAATRPRFAILPMAWNEEYVLIAPEGISADRH
                     DLVAAVRTDATPAENDGCPATTGRQAKLSGPRRVAYRRGDPTGEALAARLIGSGALGH
                     GVFAVALSPVGWDRAVETGSEWAMIVRRAAGAPCEPSWGQSTPLIAVRASVVLDKSLL
                     PVRLGGDGAPRLGDARMAP"
     gene            complement(10903..>11834)
                     /locus_tag="VFU75_01105"
     CDS             complement(10903..>11834)
                     /locus_tag="VFU75_01105"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEU5465456.1"
                     /translation="FQADQQSWNNWMGIFWRKPTKVYNQLQWNFNWWQWWSAAGLSTE
                     RAANTNVHVKFANLWWLHAGGTYGQLGTTWCDRCARGGPAVRQDPYLAPWWGFQGDDR
                     HKLIPSFFSNMFFGDAGHNSSINLQPQLTYNASTQFNVTIGANITTQHADNQWYGHYD
                     TLLATGQQHYTFAHLDQRTLGLTADLGYTITTNLSVQWHIAPFVSRGTYSNIRELANP
                     ASTSYDARYQPYADTSVANHPGGLDFKQFNSNLVVRWEYRPGSTIFVVWTQGRQDFQS
                     IAGPDGLRGDFNNLFNLHPNNTFLVKASYWLNW"
BASE COUNT         1898 a         4046 c         4003 g         1887 t
ORIGIN      
        1 ggatgatcga ctcggccccg agctccacca gttgccgccg ggcccgagcc ggcaaggcgc
       61 gagcagcggc ggtgtcggga cgcaatccat cgacgaattc catgcacaga acccgggcgc
      121 ccgagtactc cggatacacg accgggaaag cgacgcccgc gaggtccgcg aagttcgcgg
      181 cgaaggcgcg tgcattctcg gcctcctgct tcaggtccac ttctcgcagg gtgtaggcga
      241 cgaactcccg gatgatgcga cgtggttgat agcggccgaa cggccactcc agcacggcgc
      301 cgaggatgcg gagcagcagg gcatcccgtc gcaacgtctc gacgatgccc ggcttcaccg
      361 ccttgatcac gaccgggtcc cccgcgcgcg tcgtcgcccg gtgaatctgg gcgatggagg
      421 ccgacccgag gggttccgga tccacgcgga ggaacagatc gtcgagcggg cggccgagct
      481 cgcgggagac cagcgtctgg aagtccgcga acgggatggc gggcagccgc cccagcaaat
      541 gcttgagctc gtcggtgacg gctttgggga ggagatcctc tcgaagactg aggatctggc
      601 cgagcttgat gtaggtcggt cccagcagct cgagccggcg gcgcagatgg accgggaagg
      661 gcacccgcgc cagatccggg cggaggagag accggacgag gggcgacagg ccccgcgcca
      721 ggaggtaccg gggaccccgg ccggtcccgt cccggtgcct cgcatccgcc caggccacca
      781 agctgccggc caacagccca gcgaggtgcc ggttcactga cagggcgcgc ccgagcagac
      841 tcggcggggg ggcggggtcg acgtaggtaa tgggctccgg cgccgggccg cgccgccggc
      901 tcgctgcgcg gcgcctgcgc gtgaccggtg cggacggcgc gcccatcagc gcttccgccc
      961 gtagcgttcg tgggctgaga cccattcgag cgaggaaatg gcgccggcca gcgagatctc
     1021 cccggccagc accacgcccg ccacgatctc cgcaagcttg ttcacgcttc cggcgtcggt
     1081 gcatccgagc acggcgagac attcacgctg ggtggggagc ccggtgccgc cgccgtgcgt
     1141 cgcgacgatg agcgatggaa tggtgatgga caggtagaag tcgccggcgt cggtgacttc
     1201 agagtagatg atggccgcgg acccttcgga gacgttcgcc acatcctgtc cggtcgcgat
     1261 gaagagcgcg gtgatggcgt tggcggagtg cagaccgttg ttgttcgcgc ccgacaggaa
     1321 cgcacccacg ttgccgacgc cccagtgatg ctgcagcact tcgggcttca cacggaggtg
     1381 tgtggcgagt acctcccgtg aaatggtcgc ctcggccgtt acgcgtttgc cgcgcgttcg
     1441 catcacgttc acctgcgagt acttcttgtc ggtcgccaga ttggattcga ggtagaaccg
     1501 ccgaattccg tccgcatgct cgagaatcca ctggcaggcg gcgaacgtcg cccggcccac
     1561 catgttctgg cccgccgcgt cgccggtgac aaagttgaac cggagatacg cgaacttgtt
     1621 cgagaggtag acgtcgatgt agcgcaggcg ggccacgtgc gacgtcgctt cggcttcccg
     1681 ccgcagatcg tcgaggtgct cgttcaccca gagctcgaac gcccttgcct cgcgcgccgt
     1741 atcgaacacg aacacgggcg cacgctgcat cccgtcggcg gataccgtcg tgagcacacc
     1801 ccccgagagg ttcaacacct tcatcccgcg attgtacgac gccaccatcg tgccttcggt
     1861 ggtggcgagc gggatcagga actctccctg agcgtactcc ccgttgatgt ggaggggccc
     1921 ggcgaatccc accggcacct gggcgacgcc ggtgaagttt tcgatgttcc cccgtgtctc
     1981 gaggggatcg aatgaatact gcgacagatg atggagggtg gctccggtgc gcgcctcgac
     2041 gtactcgcgc cggcagcgga tcaacgactc atcgtaatca tcggtcccgt ggcgcgagct
     2101 cgggattccg gaacggatgg tgggcaccag cagtccggtc gtctccatat ccgggatact
     2161 ggcgcggcat gcgtgaaggg aaccggaaac gctcatgaag ccttcacgga ttcgttggac
     2221 gccggtggtt accgtgagcc catgccccaa gtcctctcgt cacgccagtt cgcgacgctg
     2281 gcagcggtct gcgaatcgct gctgccctcc attcaggtcg acgacgaccc tgaaggactc
     2341 ttcgcgctcg gcgcggtgca ggccggaact gcgggacgtg cggcggacct ggtcggagcg
     2401 ctccgtgacc ccggtgacgc ccgccgcctc cggttcctgc tcacggccct cgatactccg
     2461 tgggcgggac tcttcctggc cggcaagttc gctgggttcc gcgccctgcc accggcagcc
     2521 cgcgagcgcg cgctgtcgcg gctcgcgttc tcccggcttc ccccgctgcg cgccggcttt
     2581 caggccctca agcgtctcgg ccatgtcgcg cattactgct ggccggtggt gaatggggga
     2641 gcccccaccc accccgcctg gcgggcagtg ggttaccccg gacccctcgc ccctccagcc
     2701 catccgcctc taccagtcag tgcccgccga gtggagaccg acatggtcat cgacgccgat
     2761 gtggtggtcg tcggatccgg cgccggaggc ggcgtggccg ccggagtact cgccgaaggc
     2821 gggcggcacg tcgtggtgct cgagagaggg atcaacccgg ctcccgagga cctgacgcag
     2881 atcgaggggg acatgctcgg ccggctgtac ctcgatggcg gcctcatgat gacgcagtcc
     2941 gggtcgatcc cgatcctcgc cggcggatgc ctggggggcg gcaccgcgat caactacacc
     3001 acgagcttcc ctctcccacc tgagacccgc gcggaatggg acgagatatc gggccttcgg
     3061 ttgttctcca gcccgcggtt cggtgattcg ttggaccgcg tgatgcggca gcttcgtgtc
     3121 ggtacgactt ggtctacgcc gggcgagcgg gaccagctgc tggaacgggg gtgccgcgcc
     3181 ctcggctggc acgtgggcgt catcccgcga aacgtgagcg gatgctgcga gggcgtcgaa
     3241 tgcggttatt gcgggtacgg gtgccgccac ggcgcgaaaa actcgaccgc gcggacctac
     3301 ctcgccgacg cggcccgacg gggcgcggac atcatcgtgg gcgccgaggc agagcagatc
     3361 ttgatcgagc ggcggcaagt gaggggagtg gcagtccgcg cccgcgggga gaacggcgcc
     3421 gaacaccggc tcgtggtgcg ggcgcccgtc gtcgtagtgg catgtggatc gatcaatacc
     3481 cccgccctgc tcctccgatc gggactgcgc ggtccccgca tcggtcgggg actgcgcctg
     3541 catccggcca gtgccgtcgc cggagtcttc ccgtaccgcg tggagccctg gaccggctca
     3601 cttcagaccc ggtattcgga tcaattcgct tcgatcgatc aaggctacgg tgtgaagttc
     3661 gagaccgcgc cggtccactt cgccctgccg gcgagtggat tcggatggga gtcttcccgg
     3721 cgtcaccggg aggatatcgg gcgtcttggg cacaccagca tcatcggctt gctgctccgg
     3781 gatcgagatc ccggacgggt gacgatcggg ccgcatgggg gcccccgggt tcactacgag
     3841 ctttcccgat tcgacgaggc ccatatccgc cgcgccatcg tgggtggggc ccaggcgctc
     3901 gccgccgcgg gcgcgacgga gatcttctcg ctgcacacgc ctccagtccg cggccgaccg
     3961 ggtactcccg gatggctgga ccggttcggc cgggaagcgg actcccgagg ctaccgccgg
     4021 tgccggatgt cctacatcag tttccatcag atggccacgg cggccatggg ggcgacaccc
     4081 ttgctgggtg ccgtggatga agccggcgaa tcgttcgagg tcggagggct ctacgtcgcc
     4141 gatgcggcgg ggttcccgac gtcgagtggc gtgaacccca tgatcacgat catggccctg
     4201 gcggaccacg tggcgcgggg aatcctcgag acatcctgat ccccgcgcca cacacccgtc
     4261 acttccaagt ctcgacttcc ctcatgaact cgagggcctg cgccgcgagg tccaccgccg
     4321 gcgtgcccgg agcctccgtc agcgaggtca cccgggtcag gttggtctgc gccctcgtgt
     4381 acccagaatc ggccgacaac gccgacttgt aggcctcgac cgccgccgtg tagtgaccgg
     4441 tcttctccag cgcgacccca agcgagttcc ggaacgtggc cgagttcgaa agctccacgg
     4501 cacgggacag cgtcttgagc gcctcgtcgt attccccgcg ctcgatttgc acgagcccga
     4561 ggttgttgat ggaccagcca tcctggttgt tcaggacgat ggcgtgctgg tagtcggcga
     4621 ccgcggagtc ggtctgcccc aggaagtcgt gggcgcgacc caggagccgg tacgccgacc
     4681 ccgaagtgga gtcgatcgcg atcacctgct ggatctcgtc gatggcgtcc ttcgccttcc
     4741 cctgctcgat cagcacccgg gaaaggttca gccgcggggg aagcttggtc gagtcctccg
     4801 cgatggcggc gcggaattcg gtcacggctc catcccggtc gccaccacgc caggcggtga
     4861 ccgccagctt gtagtggctg acggttgggg gcgtcgggcc cgggggggtg accggcacgg
     4921 ccggaccagt cggcgtcgcc ttggtgctgg taggcagtga agcggtggtg gtgccggtcg
     4981 tcgacttgtg gccacagccg acggcgaacg cggcggcacc catggccagc acaaccagtg
     5041 tgatgccgac ggcgcggcga gtgggcgaag gctcgaagcc ttcggcgagc gagaagcgat
     5101 acatgcgtcc tccaggacgg ggttgccgtc actagaggca cgtcaggtgc caggggtcag
     5161 gggtcaggta tcaggggtca gggactgcac ttaggagaaa atatgaattg ggttcgtgtt
     5221 cgggtgaaca cgatctcagt cgagtctcgg tgttcgtccc gacacagtcc cctactgact
     5281 tagcgactta gcgactcaag tgatacagtg caatcgcatt gtcccgcaga accttcttcg
     5341 ccaggatgcg ggcttgcgcc atcgtgatgt cgccatcccg gaccatcgcc gtcagggcca
     5401 tcccaagagc tcggcgcgcg gtgttggacg caacccaggc ccccatctcc caggtctgct
     5461 tggggccgcc gtcgaaggcg tcggtgccga acaggacctt gtcgggccag cgcatgagcc
     5521 agccccggag caccgtcgcc agctcttcgg gggaaaggat gaggtccatc atcgagatgt
     5581 cggcgtagac gttgggcttg gcgagcatcg cttcggtctc acccacgtga ggccagccgc
     5641 catgaatcag aacgaagttg gtccgtcgca acgtcgagtc gttgaacacg ggctcgagca
     5701 gccccggcct cgccccctcg gcctcgtaat aaccaccgaa gaactcgagg gcgtgcaggt
     5761 gcaccgccat ccccagcttc cccgcctccc gagctacgtc ccggaagagg gcgtccgaga
     5821 ggattttcac ttcctcggcc gagggcgtac cgcccccggc gaactgagcg tagatggccg
     5881 cggcccgggc ggtatcgggt gccgcgaaat cgaaggaccg aaggtaggcc acttcgaact
     5941 tgattgcgcg agcgccggaa ccgtgctggc gctggagcag gggcacgacg acctggctcc
     6001 ggaattccgc caaggtcggc ggtaacgcgc tgagccccag atcgcccaag tatctcctga
     6061 gcaacctcgc ttcgagcgga tagagcgcgc gggtgtcggg tgtctgtccg acgagccgag
     6121 tgtcgagggg gaagatgagc gggtcgtcga acgggaccca gcggaaccgg ccgctgtcga
     6181 gccccgcacc catggcgatc cggttggcga attcgagctg gatcccgacc cggtcgagcg
     6241 cctgagccgg ggtggtcacc ccggccagcg catgggcggc ctgtccccac gccggagctt
     6301 caagcgtgag gcgccacggc agagggaaat ccgggattcc accgagaggc aaggcgtcgt
     6361 agtcggtatc tggcgcgccc cctggcggca cgacggccat ggggtgcgcg tgattgtcga
     6421 tggctttgat gctcgcgatg tacgcagcga gggacggatc gacgggggtc tgcgctcgag
     6481 cctgagccgt gatcaagaga gcgagaacga aaaagggccg cattcgaagg agaatgcggc
     6541 ccccggaaga tcttggctag tttctcctga tcccgtgccg tctgaggatc ttcagcaagc
     6601 tggcgtggtc tgccagccct aactcccggg ccgtccgggt gatatgccag gcattccggt
     6661 cgagcgccgt cagcaagatc cgcctctccg cctgcgcccg gagctcccgg aacgtccccg
     6721 ccatcgcgcc cgtttcggcg gcaggagcct cccgcacttc ccgcggaatg tccccaaccc
     6781 ccagcacctc gctgtcggcc gcgatcagca tccgctccac cacgttcttc aactcgcgga
     6841 cgttgttccg ggaccagtcg gctcgcgcga gcacttccac cgcgtccggc tcgatgggcc
     6901 ggggccgcat tccgaaccgc gaccccacca cgctgatcag atgctccacc agcgccggga
     6961 tgtccgatcg gcgctccgcc aaggccggca cacgcacgac gtgcacacag agccggtagt
     7021 acagatcgag ccggaaacgg cccgcctgga cctcctgttc cagctgacga ttggtagccg
     7081 caaccacccg cgcatcgacc ggaatgggcc ggtttccgcc gacgcgcgtg atctcccgtt
     7141 gctcgagcac ccgcaggagc ttggcctggg ccgcgagggg caactcgccc acctcatcca
     7201 ggaatagcgt cccccctgcc gccgtctcga acgcaccctt ccgcatcgtg tgcgcccccg
     7261 tgaacgcgcc ccgctcgtga ccgaagagct cgctctccac cagctcactc ggcagtgcgg
     7321 cgcagttcac cgccaccagc aatcccctgc gcgccgagac acggtggatt tcgcgggcga
     7381 cgagctcctt gcccgtgccc gaatcaccca gcaccaggac ggcactgggg atcggggcca
     7441 accggttgag ctgctcccgc acccgcgtga gggccgccga ggtacccacc agcgcccctt
     7501 cggaggcgcg gagcgtggcg acttcgcgag tcaggcgctc ccgctcgagc acgtgctcca
     7561 cctcgcgcac cacgcgctcc atcggttcgg ccttgtcgag aaagctccgg gcgccgagct
     7621 gcacggcctg gaggcaccgg tcgtagctcc cggtccctgt gtacacgatc acgggcaccc
     7681 ggcgcccgac cgacttgagg accgcgaagc cgtccaggac gggcatttcg agatcgagga
     7741 tcatcacgtc gatggcctct cgctcgatga cgtcgatcgc ggcctgccct ccggcggcga
     7801 ccagggtctg gtacttcccg agccggcgca gatcgtaggc gtactgctcc gccatggccg
     7861 gaacgtcgtc taccaccagg accgtagtca tcggtcacct gctttctctt ctcctgcgac
     7921 tcccgcggtc tcgaggcgcg ggaattccac cgtgaatgag gaccctgctc ccggtgcggt
     7981 gtccacccgg agagctcctc cggcatctcc caccagcctg cgcacaatcg tcaacccaag
     8041 ccccgacccc tgcggcttgg tcgagtgaaa tcctgaaagc gcgtgctcca gctgtgcacg
     8101 gctcatgcca gccccggtat cgatcacctg caaacacacg tgctcgccac ccctggtccg
     8161 cagcgtcacg actcctcgtc cattcaccag gctctcgcgg gcgttgctcg cgaggttgtc
     8221 gatcacccgc cgcagcgcga gcggatcggc tctgaccacc ggcgccggct cggccagctc
     8281 gacttgcacc gtcactcctt catctcccgc cagccgctga gccgcgccct gaaccgccac
     8341 gttcaagtcg cagggctcta ccacgccggg gaccgccagc cgggcgtact caccggcgag
     8401 ggcgtggaga tacgtgatac tcgactcgac cgtcccctgc cgttcggcga acacccgcgc
     8461 gagctcttcg ggactctcac ccgccgcatc gctccaatgt cgaaagacgt tccggatcgg
     8521 cgtgaggcca ttccggatgt catgattcac ctgccgcgcc aggtccccca gcgccgcccg
     8581 acgctcggcc cgccgcgccg tctcggcgct ttcgcgcaga cgggcggtca tggtgctcag
     8641 cacgccggcc agcttcccga cttcgtcccg gggagccaac cgcggcgtat cgaaggcccc
     8701 ctccgggtgc tccggatcca ggtcttccgc ccgcgccgtg aggtccgcga gtggcctcga
     8761 gagcctcccg gccagccaga gggcgagcgc cagccccgtc aacgcggtgg tgaagagtgc
     8821 cagcgcgagc caccggttca ggctgcccga aatcgccgtc agaggcgcga gggactgatc
     8881 gaccaccagg ctcgccgtgc cgaccgagtc ggcgtcttcc gacgacagaa acggcagcgg
     8941 catcacgtgc gccgcgggaa ccgcgctgtc agcgagagcg acggagacat ccgcatcggg
     9001 cgccgaacgg gccatccact ccgaatcgag tccgatgccc cccacgatcg ggtactcccg
     9061 gtccccaacg cgcagcgaat cgaggcgcgc gagggtcacg aacgtcgact ccgccgaccg
     9121 cacgcgtatg agcatgatcg agtcgggggt cgcgcggagg tgcgccggca ggctcgactc
     9181 cgcccatccg taggcattgc ggaagtgccc agacgacagc acggtgccgt cgggcgaaag
     9241 gatctggagg ctcgcgagcc ccgacacccg catggcggtg cccgcgaagt ccctgacgta
     9301 gtcgtcaccc gagtcggccg cgagggcggc gcgcaggtgc aggtcggacc cggcggcacg
     9361 ccggacccct gccaggcgcc gggcgaggtc ggcgtccgtc cccgcgaccc gcgctcggac
     9421 gtggtccagc gccgcatcgg cccgtctctc gaacatcgct tccagcctgc tgccgaccgc
     9481 ccgccgcact ccccacccga acaccacgag ggggaggaga acggcgggca ggaaggcggc
     9541 cgcgaggcgc gcgcggaagg tcacggggcc atccgcgcat cacccagtcg gggagcaccg
     9601 tcgccaccca gacgcaccgg gagcaggctc ttgtccagga cgacgctcgc acggacggcg
     9661 atcagggggg tggactggcc ccacgagggt tcgcagggtg cgccggcggc ccggcggacg
     9721 atcatcgccc attcgctccc agtctcgacc gcccggtccc accccacagg cgaaagcgcc
     9781 accgcgaaca cgccatgtcc caaggcccca ctgccgatca gccttgcggc cagggcttct
     9841 cccgtcgggt cgccgcggcg ataagcgacg cgacgaggac ccgagagctt ggcctggcgg
     9901 ccggtggtag cgggacagcc gtcgttctct gctggggtcg cgtcggtgcg caccgccgcc
     9961 acaaggtcat gccgatccgc cgagatccct tcaggagcga tcagtacgta ctcctcgttc
    10021 cacgccatcg gaaggatcgc gaaccgtggc cgggtggcgg cgaacgcaag ggccgcccgg
    10081 tcaccggtga tcacgacgtc cgcgtgactc tcgagcgcgt cgcgggcgtc accggtcccg
    10141 cgcgcaaagg tgatccgcgg cgccgtgccc ggagtggtcg gctgcaggac cgcgtcgtcg
    10201 gcgtcggtcg ccgggttcgt gagccaacgg ctccccgtcc cgatgggcca tccgtgatcg
    10261 ggcgcgggtt tggtgatggc cgccgacggt gccagcacgg ccgccagcgc gtcgccactc
    10321 cccgtcgaga tccggatcag tgtgtcgccg gcgacggtcg tccgctcggc gagggtggaa
    10381 tccgtgaggc gccacgaact tttcacgtcg gcggcgaaca cgggcgctcc atcccagaag
    10441 cgcgcgtcct tgcgcagcac gaactccacg ccgtcagtgc ccgtgcgcca cgaggccgcg
    10501 agtccgggga gcagatttcc ctggcagtcg actttgacca gcgtctcata cgcgagtccg
    10561 aaagcgatgc gctccgcggc cgagcggggg accggcgcgt cctgactctc gaccggacca
    10621 tcctcgacga cgcgaaggga gtcgcgtggt gcaccgggtg tgcccgccgc cacgcagttg
    10681 gccgggaagt gattcgccgc gggtggcgac acgcgcatga cggaatgcgc gcacgcgaaa
    10741 atccccgcca ctgcgatggt ggcgaggaag gattgagcga tcgtcgtctt cgacacgggg
    10801 acggctccgg ggagggtcgg ggctcgggat gttatacccc gagtaccccg tctctaccca
    10861 tccagacgag ccttgtgcct gctgggtttc tgcgcggtta ccctaccagt tcagccagta
    10921 ggaggccttc accaggaagg tgttgttcgg gtggagattg aacaggttgt tgaagtcacc
    10981 tcgcagcccg tcggggcccg cgattgactg gaagtcctgc cggccctgcg tccacactac
    11041 gaagatcgtg gaaccgggcc ggtactccca ccggaccacg aggttggagt tgaactgctt
    11101 gaagtcgagc ccgcccggat ggttcgccac cgacgtgtcg gcgtagggct gatagcgcgc
    11161 gtcgtaagag gtcgacgcgg ggttcgccag ctcgcggatg ttcgagtagg tcccgcggct
    11221 cacgaacggc gcgatgtgcc actgcaccga gagattcgtc gtgatggtgt agccgaggtc
    11281 cgcggtgagg cccagggtgc gctgatcgag gtgcgcgaac gtgtaatgct gctggcccgt
    11341 ggcgagcagg gtgtcgtagt gaccgtacca ctggttgtcg gcgtgctgcg tcgtgatgtt
    11401 cgcgccgatc gtcacgttga actgcgtcga ggcgttgtac gtcagctgcg gctgcaggtt
    11461 gatcgacgag ttgtgcccgg cgtccccgaa gaacatgttc gagaagaacg agggaatcag
    11521 cttgtgccgg tcatccccct ggaagcccca ccagggcgcc agatagggat cctgccgcac
    11581 cgcggggccg cctcgtgcac agcggtcgca ccacgtggtg ccgagctggc cgtaggtccc
    11641 gccggcgtgc agccaccaca ggttggcgaa cttgacgtgg acgttcgtgt tcgcggcgcg
    11701 ctcggtggag aggccggccg cggaccacca ctgccaccag ttgaagttcc attggagctg
    11761 gttgtagacc ttggtgggct tccgccagaa gatccccatc cagttgttcc agctctgctg
    11821 gtcggcctgg aaca
//
LOCUS       DASUBP010000025        11514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_150553, whole genome shotgun sequence.
ACCESSION   DASUBP010000025 DASUBP010000000
VERSION     DASUBP010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 11514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11514
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150553"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..1488)
                     /locus_tag="VFU75_01110"
     CDS             complement(52..1488)
                     /locus_tag="VFU75_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465457.1"
                     /translation="MRAARWIPWFGGALTTAWVLFVAGPRPGLTPDAMSYLGAAESLV
                     HQGSLREPFATWNAPDSTTPLTDYPAGFSLVLAPPIAAGVPGPRAAAWVEALSVGGAV
                     GVASALVGSVSGELGALVTVVLLALMPALVDVSFMVVSEPLFLLLLSLTVAGMVRRPE
                     RPELSGLAAAGANLVRYAGVFLVAGAALWQAFQPGTWQSRLRRLAIAALPGVLLHLYW
                     RVEGIVPGGGFAAGVYTGWPEALSEAWNTGASWLAPGLHGWVATVIAIVLLGAMATAW
                     FRGGPLVAAAGLLSILYVGTLAFAKTFVIADLPFDFRMLLPLCFLLTLGTVPNLVAKS
                     GGGRAAAALVVTVWGAMALTRDIREIHRTRIGGVDYETAEWQESGVAAWLRGPGRGMA
                     LYSNDPAGIWFTAGRPSRLLPRSGAPDSISAFRARFSAQPSALVAFGETFALESVEAD
                     SLAADLHLIPVATFKHGVVWMTAKSLGR"
     gene            1589..2164
                     /locus_tag="VFU75_01115"
     CDS             1589..2164
                     /locus_tag="VFU75_01115"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HEU5465458.1"
                     /translation="MNLPTRTLGLVLLMAMPLAAQQPMMADSGHHAHGDMMMDHGMMG
                     PGMPDMMMMMGDMEYSPEHLLAMNGELSLTAQQIMALTALRDQAKKSRDGTMDKMKAH
                     MGELRTALDAATPDTTAIRTHFLAAHDAMGQAHLAMLITNARAKAILTDAQRAQLSQM
                     HKMRMMNRRGPGRAGPDGPAGWRGRPAMRVF"
     gene            complement(2171..2518)
                     /locus_tag="VFU75_01120"
     CDS             complement(2171..2518)
                     /locus_tag="VFU75_01120"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEU5465459.1"
                     /translation="MRLSNIAAVAALLVIAGCKSSTSPSTGGRSLNIGVENNFYSLTP
                     DTIASGATVTWTWGASSNGHTVNWDSGPGTLPANSATMTSGTYTATLNTPGTYHYHCL
                     VHGAAMSGVVVVE"
     gene            complement(2642..3097)
                     /locus_tag="VFU75_01125"
     CDS             complement(2642..3097)
                     /locus_tag="VFU75_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465460.1"
                     /translation="MTRWTWGLAVVVAGALMAGRPVAAQSPAYGLHFGYNFDVDDPLA
                     GAQATFPFSRYVAFYPSFDYYFTSPVHTWGLNGDLKLYPGSYRMFYVAGGINYTHVSL
                     NGNGSGTTNLNLVAGLEGHRRFGAPYLEGRLILGNGSSSFQIVGGLNWR"
     gene            3142..4623
                     /locus_tag="VFU75_01130"
     CDS             3142..4623
                     /locus_tag="VFU75_01130"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEU5465461.1"
                     /translation="MTLLTLRGITKRYPGVTALDAVDLDIDPGTVHALVGENGAGKST
                     LLKIVAGAERPDAGVMTWKGQTIAAPTPREALLRGVTVIYQELMLAPNLSAAANIYLG
                     TEPGHRGLLDASAMRHRAASALADLGLVIDPDQPVGELSVAQRQLVELARALVRDAQL
                     VALDEPTATLTAHEVAHLVAQIEWLRAKGIAVVFVSHRLDEIRRLADSITVLRDGHRV
                     WTGPAAGIDERTLIRTMVGRDVVFERCPPNRKADAEPVLSVRGVSRGRALRDVSFDVK
                     RGEILGLGGLVGAGRSEVARVLAGIDRPDAGSLTLRGQAYRPRSPADAIRAGVVYFPE
                     DRKRQGLVLGMRIRENVTLPVLSRVSRGGLLQAGAERHLAQAAATRADVRPPDIERLA
                     GTLSGGNQQKVVLAKWLLTEAEVLIFDEPTRGVDIGAKTEIHRQIRALADAGKAVIVI
                     SSELPELIALADRAVVMREGRVQGELSEPLTPEAVMALAFRSA"
     gene            4620..5657
                     /locus_tag="VFU75_01135"
     CDS             4620..5657
                     /locus_tag="VFU75_01135"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5465462.1"
                     /translation="MIAHTRSWMTRYGVLAALIAMSVVATIVAPGFFSLANASTILLH
                     VSINGILTLGMTFVIITAGIDLSVGSLLGLAGVLVAGALTAPGLIAVVGVGGAAVVAT
                     LIGLAVGAAVGIANGALTAYLSINPFIVTLAMMTVARGAARLVTGGVPIGFPPMGDPH
                     FHDKVAALDALHAFGGGRIPIPGTAQGFPVPALVMLALVFLSALILDRTRFGRYVYAV
                     GGNEEAARLSGVPVRGVKVGVYAIAGACAGLAGVLMVGQLRSGGPDAGLLYELNAIAA
                     AVIGGASLMGGIGSAWGALTGALLIGVLNNALDLMGVQAFWQEIAKGAIILLAVLFDV
                     WTKQRNGGTTQ"
     gene            5654..6574
                     /locus_tag="VFU75_01140"
     CDS             5654..6574
                     /locus_tag="VFU75_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEU5465463.1"
                     /translation="MIKRNLGVSAVLALAAFTLSCGHKKHDRPQVGVTLLTEAHVFYQ
                     NLKQGLQQGADSLGVDLHVVAGEWDLARQTSQVQNFVTQGMDAIVIAPVNSSGIVSAI
                     EDANHAHIPVFTADISADGGQVAAHVASDNIQGGRLLGEYVATRLKGGGNVAILDQPT
                     VASVRDRVTGFVDALAKYPNIRIVARPAVERGLRDVAKQKMDNLLTTDQHIDAVFGTN
                     DDCALGALAAIQAAGTKNVFVVGYDATPEARTEIASGTALVADAVQDPVTIGRRTIET
                     VVEVLKGQSVPAIIPVPVGIVTRDSLTLKH"
     gene            6585..10415
                     /locus_tag="VFU75_01145"
     CDS             6585..10415
                     /locus_tag="VFU75_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU5465464.1"
                     /translation="MAAPESALSRRSTIAPISANEREERAQREIKVGLTDRLVSEQRG
                     ALFAHLVLLAVSVWLLAPVARPPLLMAWAGAVLIAVLVRVVLMTRLKSIHLTPQAATS
                     SVRLSVLGLGLAWGVGVAVMTPEVPIRYVTLLLVTEAGLVSGAAWTLTSDLWSFRIFL
                     ITMLAPLPIGIMFNATPDSIRAREAAVILIILYMAYMWILGQRSHLSLVTTLRAGALL
                     RIREEETSRQNAFLGALFASAPIAMAEVSEDGLIERINPRFVTLFGWAEHEAVGQMLD
                     GLMVPLDERMESVALHHGILQGRPMAKDLRRMTKSGNVIDVLVTAAAVNRSDGSRGIV
                     ALYEDVTERRRSEAQVRELSERLAAVLGAATQVSIIATDLDGIITVFNAGAEQMLGYR
                     ADEVVEIEPFERILVASEITARSRDVLRETGRQVEGFEVLVEPARRGSYDQREWTYVR
                     KDGKRLTVELVVTPLRDGNGQIAGFLGVATDVTQRNRQAAALREAEAHFRQLLDSSGE
                     GIYGVDGDGRITFFNPEASNLTGYALKDALGRDAHDLLHHTRPDGTAYLASECPISQA
                     FRTGQGIRLEEEMFWKKDGSAFPVALTAFPMRELGGGVSGAVITFADTSERVAARAAI
                     EHAREAAEQTARTRSAFLANMSHEIRTPLNAILGLTELLFDTPLAPEQRHSLELVRGA
                     GETLLTLLNDILDLSKIEADQLRLESIPFDPEHLIESTLALLAIRAREKRLDLITDVQ
                     AGVPVSVRGDPTRLRQILTNLVGNAIKFTDHGEVVVGVGKREPSTDGDVLLHFTVRDT
                     GIGIPADKLEAIFDEFTQADSSTTRRYGGTGLGLAICRRLVRLQGGELRVESEVGRGT
                     EFSFSLRFPVEKATATPALGTPAALIAKILVVDDNETNRRILREMLQAAGSHVDEAGD
                     ASTVHRTLLDAVTEKKPYALAILDGQMPEIDGFSLAAELRQDAALSELRLMLLTSAAL
                     PGDGQRCRELGIQGYLPKPASRADLLDAVASVLRGKVDRQVVTRHSMAEARRGIRILL
                     AEDNPVNQQVAATMLRKRGHQVDVVDNGRDAVAAVAKNQYDIVLMDIQMPELDGMEAT
                     AEIRKTAKGKTLPIVALTAHALTGDREKYLSAGMNGYLSKPFKPHELFAAAEGWATTD
                     GQSVAIREPETKTEVPPAADLERLRRELTEAGAADALGAILDTFLTDAPKRMETLIAA
                     VKAGDAVAISKAAHAFKSASATIGAAGLAKMLADIEHRGKAKDVDGLGAIVEAAKKEA
                     DRVLEQVNATRPTV"
     gene            complement(10491..11411)
                     /locus_tag="VFU75_01150"
     CDS             complement(10491..11411)
                     /locus_tag="VFU75_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465465.1"
                     /translation="MRNNLLAVVPAALVLIGGAVMAGCSDTSTSPASDQSDVLASLTD
                     QYGAQMDTNFGVAGGTTSDAAAPAPLRAFVAADTATLPAFWGRLRVIPGGAKPILNRN
                     IVIQGDTAKVTQTISFQGLFLVDTTKDTVFNPTSKTLDEGMTQSAVLVRDPSQTHRWR
                     AVSLTLQNWLPTDAAARTVAVTQITVKVNDTLRITADNPDSLYTVGRLLHLHVGDTVS
                     VTASVSNSDTTYSPATFVFLHVHHAFSDLNWHRVPMKNNGDGTFSRTWVVARKGIDRF
                     VIDAINAATLERGTVNNYDANEWGIPYRID"
BASE COUNT         1998 a         3920 c         3835 g         1761 t
ORIGIN      
        1 tagtcgctaa gacgctaagt cgctaagacg ctaagtgacc accttaccga cttagcgacc
       61 tagcgactta gcggtcatcc agacgacccc atgcttaaag gtcgccactg gaatcaggtg
      121 cagatcggca gccagtgaat cggcttccac cgactcgagg gcgaacgtct ccccgaacgc
      181 caccagcgcc gacggctggg cggagaaccg ggcccggaag gcactgatcg agtccggggc
      241 tccactgcgc ggcaacagcc gcgatggccg gccggcggta aaccagattc ccgcggggtc
      301 gttggaatac agggccatgc cccgccccgg accacggagc caggccgcga cccccgactc
      361 ctgccactcc gcggtctcgt agtccacgcc gccgatccgc gtgcgatgaa tctcccgaat
      421 atcacgggtc agggccatcg ctccccagac tgtgaccacc agagcggccg cggcgcgacc
      481 cccacccgac ttcgccacca ggttggggac ggtgccgagt gtgagcagga agcagagggg
      541 caggagcatc cggaagtcga acggtagatc ggcgatcacg aacgtcttcg cgaacgcgag
      601 cgtgccgaca tagaggatgg aaagtagacc ggcggctgcg accagcggtc cgccgcggaa
      661 ccacgcagtt gccatcgcgc cgagcaatac gatggcgatg acggtcgcca cccacccgtg
      721 aagtccgggt gccagccatg acgccccggt attccaggct tcgctcaagg cctccggcca
      781 gcccgtgtac actcccgcgg cgaaccctcc accggggacg atcccctcga ccctccaata
      841 gagatgaagg agcacgcccg ggagggcggc aatggcgagt cgtcgcaggc gggactgcca
      901 ggtccccggc tgaaaggcct gccagagtgc cgcccccgca accaggaaca cccccgcgta
      961 ccggaccaga ttcgctccag ccgccgccag gccggataac tccgggcgct caggccggcg
     1021 gaccatcccg gcgaccgtca gcgacaacag gagcaggaac aacggctccg acaccaccat
     1081 gaaggacaca tccaccaacg ccggcatcaa cgcgagcagc acgacggtca ccagcgcgcc
     1141 gagctctccg gagaccgagc cgacgagcgc cgaagcaacg cccaccgccc caccgacgct
     1201 gagagcctca acccaggcgg ccgcgcgggg accggggacg ccggcggcga tcggcggggc
     1261 gagcaccagg gagaagccgg ccggataatc ggtgaggggc gtcgtgctgt cgggcgcgtt
     1321 ccaggtcgcg aacggttcgc gcagtgaccc ctgatgcacg agagactccg cggcgccgag
     1381 gtacgacatg gcgtcgggag tgaggccggg ccgcgggccg gcgacgaaga gcacccacgc
     1441 cgtcgtgagt gctcccccaa accacggaat ccatcgagcc gcgcgcaccc gggaagtcta
     1501 caccctccct taatcaattc ctcacgcggt cacgcgtatc ttgagtcgaa tacatgcacg
     1561 aatccacacc tgactctcgg aggaaacgat gaacctgccc acgcggacac tcggcctggt
     1621 gctgctcatg gcgatgccgc ttgccgccca gcaaccgatg atggccgata gcggacatca
     1681 cgcccatggg gacatgatga tggaccatgg gatgatgggc cccggcatgc cggacatgat
     1741 gatgatgatg ggcgacatgg agtacagccc cgagcatctc ctggccatga acggtgaact
     1801 gtcactcacg gcccagcaga tcatggcgct caccgccctg cgggaccagg ccaaaaagtc
     1861 ccgtgacggc acgatggaca agatgaaggc gcatatgggc gagctgcgca ccgccctgga
     1921 cgcggcgacg cccgacacca ccgccatccg gacgcacttc ctcgcggccc acgacgcgat
     1981 gggtcaggcg cacctggcca tgctcatcac caacgcacgc gcgaaagcga tcctgacgga
     2041 tgcccagcgc gcccagctgt cgcagatgca caaaatgaga atgatgaacc gccggggccc
     2101 cggccgggcc ggcccggacg gtccggccgg ctggcgcggg cgcccggcga tgcgggtgtt
     2161 ctaggagcgg ctactcgacg acgacgactc cactcatcgc cgccccgtgg acgaggcagt
     2221 gatagtgata ggtgccggga gtgttgagcg tcgcagtgta cgtcccggac gtcatcgtgg
     2281 ctgagttcgc gggcaaggtt cccgggcccg agtcccagtt cacggtgtga ccgttggatg
     2341 acgcccccca ggtccaggtg acggtggcgc ccgacgcgat ggtatccggg gtgagcgaat
     2401 agaaattgtt ctcgacgccg atgttgaggc tccggccgcc ggtggatgga gacgtgctgc
     2461 tcttgcaccc ggcgatcacc agcagggcgg cgacggcggc gatgttggac aaacgcatgc
     2521 gattcctccg acaaggtgac gaaccaagga gcgatggtcc gtactaccgg accaccgctc
     2581 cttttgtccc ctcgttttca gttgccggaa cgagccctga ccgggtcctg accaccggcc
     2641 cttagcgcca gttcaaaccg cccacgatct ggaacgacga ggacccattc cccagaatga
     2701 ggcgtccctc gaggtagggt gcaccgaagc gccggtggcc ctcgagtccg gcgaccagat
     2761 tgagattcgt cgtaccgctg ccattcccgt tgagactcac gtgcgtgtag ttgatcccgc
     2821 ccgcgacgta gaacatgcgg tacgaacccg gatacagctt caggtcaccg ttgagccccc
     2881 aggtgtgaac cgggctcgtg aagtagtagt cgaaggacgg ataaaaggcg acataccgcg
     2941 agaatggaaa cgtcgcctgt gcccccgcca gcggatcgtc cacgtcgaag ttgtaaccga
     3001 aatgcagccc gtaggcgggg ctctgagcgg ccaccggcct gcccgccatc aaggcgcccg
     3061 cgacgactac cgcgagcccc cacgtccatc gagtcatcgg agtctcctga aggattcctt
     3121 tgcagtgtac attgagccgc agtgaccctg ctcacacttc gcggcatcac caaacgctac
     3181 cccggcgtca ccgctctgga cgcggtcgac ctggacatcg atccgggcac cgtgcatgcc
     3241 ctcgtcggcg agaatggtgc cggcaagtcc acccttctca agatcgtggc tggagcggag
     3301 cgtcccgacg ccggcgtgat gacgtggaag gggcagacga tcgcagcgcc cacgccgcgc
     3361 gaagcgctgc tccggggcgt caccgtcatc tatcaggagc tgatgctcgc gccgaatctc
     3421 agtgcggccg ccaacatcta cctcggcacg gagccggggc accgcggact cctcgacgcc
     3481 tcggcgatgc gccaccgggc ggcgtcggcg ctcgcggacc tgggactcgt catcgacccg
     3541 gaccagcccg tcggcgagct gtccgtcgcc cagcggcagc tcgtggagct ggcgcgggct
     3601 ctggtccgcg atgcccagct cgtcgcgctc gacgaaccca ccgccaccct caccgcccac
     3661 gaggtcgccc acctggtggc gcagatcgag tggctccgcg ccaaagggat cgccgtcgtg
     3721 ttcgtgtccc atcggctgga cgagatccgg cgcctggccg actcgatcac cgtcctgcgg
     3781 gacggccatc gcgtctggac cggtcccgcc gccggcatcg acgagcgcac gctgatccgc
     3841 accatggtcg gccgcgacgt cgtgttcgag cgctgccctc ccaaccggaa ggccgacgcc
     3901 gagccggtac tatcggtgcg gggggtatcc cggggacgcg ccctgcggga cgtctccttc
     3961 gacgtgaagc ggggggagat actcggcctg ggcggcctgg tcggcgccgg gcgttccgaa
     4021 gtcgcgcggg tgctcgccgg catcgaccgc cccgacgcgg ggtcgttgac cctccgcggg
     4081 caggcgtatc gcccccggtc accggccgac gcgatccgcg ccggcgtggt ctactttccc
     4141 gaggaccgga agcgccaggg tctggtgctc ggcatgcgga tccgggagaa cgtgacgctg
     4201 ccggtgctgt cgcgggtctc ccggggggga ctcctgcagg ccggcgcgga gcggcatctg
     4261 gcgcaggccg cggccacccg ggcggatgtg cgcccaccgg atatcgaacg tctggcggga
     4321 acgttgtccg gcggcaacca gcaaaaggtc gtgctcgcca agtggctgct cacggaggcc
     4381 gaggtcctga ttttcgacga accgacgcgg ggggtcgaca tcggcgcgaa gaccgagatt
     4441 caccgccaga tccgcgcgct ggcggatgcg ggcaaagcgg taatcgtgat ttcgtccgag
     4501 cttcccgagc tcatcgccct cgcggaccgc gccgtcgtca tgcgtgaggg ccgcgtgcag
     4561 ggcgagcttt ccgaacccct gactcccgag gccgtcatgg ccctcgcctt ccggtcggca
     4621 tgatcgcgca cacccgctcc tggatgaccc ggtatggcgt cctggccgcg ctgatcgcga
     4681 tgtcggtggt cgccaccatc gtcgcccccg gcttcttttc actcgccaac gcttccacca
     4741 tcctgctcca cgtctcgatc aacggcatcc tgacgctcgg gatgacgttc gtgatcatca
     4801 cggcgggaat cgacctgtcg gtcggatcgc tcctcggtct ggcgggcgtg ctcgtcgccg
     4861 gcgcgctgac ggcgcctggc ctcatcgcgg tcgtgggagt cggcggcgcg gcggtcgtgg
     4921 ccaccctgat cggcctggcg gtgggcgcgg cggtcggcat cgcgaatggt gcgctcacgg
     4981 catatctgag catcaatccg ttcatcgtga cgctcgccat gatgaccgtt gcgcggggcg
     5041 cggcgcgact cgtgacggga ggcgtcccga tcggctttcc gccgatgggc gacccgcatt
     5101 tccacgacaa ggtcgcggca ctggatgccc tgcacgcgtt cggcggcggg cggatcccca
     5161 ttcccggcac cgcgcagggc tttccggtcc cggccctcgt catgctcgcc ctcgtcttcc
     5221 tcagcgccct catcctcgac cgcacgcgtt tcggccggta cgtgtacgcg gtcggtggca
     5281 acgaagaggc ggcgcgcctt tcgggcgtgc cggtgcgcgg ggtgaaagtc ggcgtctatg
     5341 cgattgccgg agcctgtgcc ggactcgccg gcgtgctgat ggtgggccag ctgcggtcgg
     5401 gaggtccgga cgccgggctc ctgtacgagc tcaatgcgat cgccgccgcc gtcatcggcg
     5461 gagcttccct gatgggagga atcggctcgg cctggggggc tctcacgggg gcgctcctca
     5521 tcggcgtcct caacaacgca ctcgacttga tgggcgtgca ggccttctgg caggagatcg
     5581 ccaaaggcgc catcatcctt ctggcagtcc tcttcgatgt ctggaccaaa cagcggaacg
     5641 gcggaacaac tcaatgatca agcggaacct cggtgtctcg gccgtcctgg cgctcgcggc
     5701 cttcaccctc tcatgcggac acaagaagca cgaccgtccg caggtggggg tcacgttgct
     5761 cacggaagcg cacgtgttct atcagaatct caagcaggga ctgcagcagg gcgccgactc
     5821 gctcggtgtc gacctccacg tggtggccgg cgagtgggat ctcgcccgcc agacctccca
     5881 ggttcagaat ttcgtgacgc aagggatgga cgcgatcgtc atcgcacccg tgaactcgag
     5941 cggcatcgtg agcgccatcg aggacgcgaa tcacgcacac attcccgtgt tcaccgccga
     6001 tatttcggcg gacggcggcc aggtagcggc gcacgtcgcc tcggacaaca tccagggagg
     6061 ccggctgctc ggtgagtacg tcgcgacgcg gctcaaagga ggcgggaacg tcgccatcct
     6121 cgaccagccc accgtcgcga gcgtgcgtga ccgggtcacc ggattcgtgg acgcgctggc
     6181 gaagtacccg aacatccgca tcgtcgcccg tccggcagtc gagcgcggcc tccgcgacgt
     6241 ggcaaaacag aagatggaca acctgctcac caccgatcag cacatcgacg cggtgttcgg
     6301 gaccaacgac gactgcgcgc tgggcgcgct cgccgccatt caggccgccg ggaccaagaa
     6361 cgtgttcgtg gtgggatacg acgcgacgcc cgaggcccgc accgagatcg cgtcggggac
     6421 ggcgctcgtg gcggacgccg tacaggaccc cgtgacgatc gggcgccgta ccatcgagac
     6481 cgtggtcgaa gtgctgaagg gacaatcagt tccggccatc atcccggtcc cggtcggcat
     6541 cgtgactcgt gactcactga cgctcaaaca ctagcgtaac agccatggct gctcccgaga
     6601 gcgccctctc caggcgctcc accatcgctc ccatctcggc gaacgagcgg gaggaacggg
     6661 ctcaacgcga gatcaaggtc ggcctcaccg accgcctcgt ctccgagcag cgcggggcgc
     6721 tgttcgcgca tctcgtcctc ctcgccgttt ccgtgtggct gctcgccccg gtcgccaggc
     6781 ccccgctcct gatggcctgg gcgggggcgg tgctgatcgc ggtgctcgtt cgcgtcgtcc
     6841 tcatgacccg cctcaagtcg atccacctca ccccccaggc cgcgacctcg agcgtccgcc
     6901 tgtcggtact gggactggga ctcgcctggg gtgtcggggt ggcggtcatg acgccggaag
     6961 tccccatccg atacgtgacc ctcctcctcg tgaccgaggc gggcctcgtg tcgggtgccg
     7021 cgtggacgct gacgtcggat ctgtggagct tccggatctt cctgatcacg atgctcgcgc
     7081 cgttgcccat cgggatcatg ttcaatgcga cgcccgacag cattcgggca cgcgaagcag
     7141 ccgtcatcct gatcatcctg tacatggcgt acatgtggat cctgggccag cggtcgcacc
     7201 tctcgctcgt cactacgctc cgcgccggtg ccctgctccg catccgcgaa gaagagacca
     7261 gccgccagaa cgccttcctg ggcgccctgt tcgccagcgc cccgatcgcc atggccgagg
     7321 tgtccgagga tggcctgatc gagcggatca acccgcgctt cgtcacgctg ttcggctggg
     7381 cggagcacga agccgtcggc cagatgctcg acgggctgat ggtcccgctc gacgagcgca
     7441 tggagagcgt ggcgctccac cacgggatcc tccagggccg gccgatggcg aaggatctgc
     7501 ggcgaatgac caagagcggc aacgtgatcg acgtgctcgt cacggcggcc gcggtcaacc
     7561 gcagcgacgg aagccggggc attgtggcgc tctatgagga cgtcaccgag cgccgccgct
     7621 ccgaggccca ggtccgggag ctgtccgagc ggctggccgc cgtcctgggc gccgccactc
     7681 aggtatcgat catcgcgacc gatctcgacg gcatcatcac cgtcttcaat gccggcgccg
     7741 agcagatgct ggggtaccgg gcggacgagg tggtggagat cgagccgttc gagcggatcc
     7801 tggtggcgtc ggagatcacc gcgcggtccc gcgatgtctt gcgggaaacg ggacgccagg
     7861 tcgagggttt cgaagtcctg gtggaaccgg cccgccgcgg ctcctacgat cagcgcgagt
     7921 ggacgtacgt ccggaaagac ggaaaacgtc tgaccgtcga gctggtggtc accccgttgc
     7981 gggacggaaa cggccagatc gcgggatttc tgggggtggc cacggacgtc acccagcgga
     8041 accggcaggc ggccgccctt cgggaagcgg aggcccattt ccgtcagctg ctcgactctt
     8101 ccggcgaagg catctacggg gtggacggag acggacgcat cacgttcttc aacccggaag
     8161 cgtccaatct caccggctac gcgctgaagg acgccctggg acgggacgcg cacgacctgc
     8221 tgcaccacac ccggcccgac ggcaccgcct acctcgcctc cgaatgtccc atctcccagg
     8281 ccttccggac gggacagggc atccgcctcg aggaggagat gttctggaag aaggacggca
     8341 gcgcgttccc cgtggcgctc accgccttcc ccatgcgtga gctcgggggc ggcgtgtcgg
     8401 gggcggtcat caccttcgcc gacacgtcgg aacgggtggc ggctcgtgcg gccatcgagc
     8461 atgcccggga agccgccgag cagacggcgc ggacccggtc cgccttcctg gcgaacatga
     8521 gccatgagat ccgcacgccg ctgaacgcca tcctcggact caccgagctc ctgttcgaca
     8581 cgcccctggc gcccgagcaa cgccattccc tggaactggt gcgcggtgcc ggcgaaaccc
     8641 tgctcaccct gctcaacgac atcctggatc tctcgaagat cgaggccgac cagctcaggc
     8701 tcgagagcat ccccttcgat cccgagcacc tgatcgagtc gacgctggca ctgctggcga
     8761 tccgggcccg ggaaaagcgc ctcgacttga tcaccgacgt gcaggcgggc gttccggtga
     8821 gcgtgcgggg agatccgacc cggctgcgcc agatcctcac caacctcgtc gggaacgcga
     8881 tcaaattcac ggaccatggc gaagtcgtgg tcggcgtcgg gaagcgcgag ccctcgaccg
     8941 acggcgacgt gctcctccat ttcacggtgc gcgataccgg gatcggcata ccggccgaca
     9001 aactggaagc catcttcgac gaattcaccc aggccgactc atcgaccacc cgccggtatg
     9061 gcggcaccgg actggggctc gccatctgcc gccggctggt gcgactgcag gggggcgagc
     9121 tgcgcgtgga aagcgaagtg ggcaggggga cggagttctc cttctccctg cgcttcccgg
     9181 tcgagaaggc gacggccaca ccggccctgg gcacgcccgc cgcgctcatc gccaagatcc
     9241 tggtcgtcga cgataacgag accaaccgcc gcatcctgcg cgaaatgctc caggccgcgg
     9301 gctcgcacgt cgacgaagcc ggagacgcgt ccacggtcca ccggacgctg ctcgacgcgg
     9361 tgacggagaa gaagccgtac gccctggcga tcctcgacgg ccagatgccc gagatcgacg
     9421 gtttttcgct ggccgccgag ctgcgccagg atgccgcgct cagcgaactg cgcctgatgc
     9481 tgctcacctc cgcggcgttg cccggagacg gccagcggtg ccgggaactg gggattcagg
     9541 gatatctgcc gaaaccggcg tcccgcgccg atctcctgga cgccgtggcc tcggtgctgc
     9601 gcggcaaagt cgaccggcag gtcgtcaccc ggcactcgat ggcggaggcg cgacgcggaa
     9661 tccggattct gctggcggag gacaaccccg tgaatcaaca ggtggccgcg acgatgctgc
     9721 gcaagcgcgg acaccaggtg gacgtcgtgg acaacggccg cgatgccgtc gccgcggtgg
     9781 cgaagaacca gtacgacatc gtcctgatgg acatccagat gcccgagctc gacggcatgg
     9841 aagcgacggc ggaaatccgg aagacggcca agggcaagac gctccccatc gtcgcgctga
     9901 ccgcgcacgc gctgaccggc gaccgcgaga agtatctgtc ggcagggatg aacgggtacc
     9961 tgagcaagcc cttcaagccc cacgagctct tcgccgccgc cgaagggtgg gcgacgaccg
    10021 acggccagtc ggtggcgatt cgcgagccgg agacgaaaac cgaggtcccc cctgcggccg
    10081 acctggagcg gctgcgccgg gagctcaccg aggcgggggc ggccgacgcg ctgggtgcca
    10141 ttctcgacac gttcctgacc gatgcgccca aacggatgga gacgctcatc gccgccgtga
    10201 aggccggtga tgccgtcgcg atttcgaagg cggcgcacgc cttcaagtcc gcgtccgcca
    10261 cgatcggggc ggcgggactc gcgaagatgc tcgcggacat cgaacaccgg ggcaaggcga
    10321 aggacgtgga tgggttgggt gcgatcgtcg aagcggcgaa gaaggaagcg gaccgggtac
    10381 tcgaacaggt gaacgcgacg cgtccgactg tttaatcgtc ggccgcgccg cgttcatccc
    10441 ttcgcgagct cagggcaagc tctgagcgga gcgaaggatc tgctggatcc tcagtcaatc
    10501 cggtagggaa taccccactc gttggcgtcg tagttgttca ccgtcccgcg ctcgagcgtc
    10561 gccgcgttga tggcatcgat gacgaaccgg tcgataccct tgcgggccac gacccaggtc
    10621 cggctgaacg tcccgtcccc attgttcttc atcggtaccc ggtgccaatt caagtcgctg
    10681 aaggcgtgat gcacgtgcag gaacacgaag gtcgccggcg aataggtcgt gtcgctgttg
    10741 ctcaccgacg cggtcacgga caccgtgtcg cccacgtgca ggtggagcaa ccggccgacg
    10801 gtatagagcg aatccgggtt gtccgcggtg atccgcaggg tgtcgttcac cttcaccgtg
    10861 atctgggtga ccgccaccgt acgagccgcg gcgtcggtcg gcagccagtt ctgcaaggtc
    10921 aaggacaccg cccgccagcg atgcgtctgc gaggggtcac gcaccaggac cgccgactgc
    10981 gtcatcccct cgtcgagcgt cttcgaggtc ggattgaaca cggtgtcttt ggtggtgtcg
    11041 accaggaaca gcccctggaa gctgatcgtc tgggtcacct tggcggtgtc accctggatg
    11101 acgatgttgc ggttcaggat cggtttcgcc ccaccgggaa tgacccggag acggccccag
    11161 aaggccggga gggtcgcggt atccgcggcc acgaacgccc gcaacggcgc gggggccgcg
    11221 gcgtccgacg tggtcccgcc ggccaccccg aagttcgtat ccatctgcgc gccgtattgg
    11281 tccgtcagcg acgcgagcac atcagactga tcggacgcgg gcgacgtgga cgtgtcgctg
    11341 cacccggcca tcacagcccc tccgatcagc accagcgccg ccggcacgac ggcgagcagg
    11401 ttgttgcgca tggcctgaat ctccttaccc aagtaggaac ggttgctgtt tcccaaactc
    11461 ggagaggaga cggcgtgagc ggtctgatgt taaggggtga cggaggtcac gaac
//
LOCUS       DASUBP010000026         3508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_682038, whole genome shotgun sequence.
ACCESSION   DASUBP010000026 DASUBP010000000
VERSION     DASUBP010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682038"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(180..719)
                     /locus_tag="VFU75_01155"
     CDS             complement(180..719)
                     /locus_tag="VFU75_01155"
                     /inference="COORDINATES: protein motif:HMM:NF024183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU5465466.1"
                     /translation="MWGLVAGVLLAALAVWVVIDPILRPRVPGPLVDAGADPDDDLSP
                     RAVALRALSEIEFDRATGKLSLEDYESLKARYTREAVNALREGDRGSISSGAPPERPA
                     APQAPSTAVLPLVAKRAADSGLTCPVHGLQPEAGAAFCSECGRRLGTEAQDRFCTKCG
                     TLLERDSSFCGRCGSRVAA"
     gene            complement(725..1345)
                     /locus_tag="VFU75_01160"
     CDS             complement(725..1345)
                     /locus_tag="VFU75_01160"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HEU5465467.1"
                     /translation="MNAGLTRRQFWGALAGGLVAGDLPARLLGQGIQRPVDTAQAPLP
                     GAGPTGTLADPARAGRPLDPVTARDNDAGIQAIEKRLSCTCGCGLDIYTCRTTDFSCT
                     YSPGYHRQILALAASGMSANDIIAEFVKEHGESILMSPPKRGFALFGYFGPWAVVAVA
                     ATGLAWLIRRWARRVGAVHAVEAPAGPAPSPEDLALVQRELSRFES"
     gene            complement(1342..3360)
                     /locus_tag="VFU75_01165"
     CDS             complement(1342..3360)
                     /locus_tag="VFU75_01165"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis CcmF C-terminal
                     domain-containing protein"
                     /protein_id="HEU5465468.1"
                     /translation="MTILGSLALWIACLMGVWGAATGFVGASTGRIELQQSSRHAVFA
                     MCLTLLVAVFCVEWLLFARDFNVEYVAAYTSRNLPTFYTWSALYAGQKGSLLFWATVL
                     SIFGSLAMVLTAPRHRVYLPYVSAVVCVVAVFFLVVMLGAANPFERLPYTPVDGRGMN
                     PQLQNPGMVFHPPMLYLGYISITIPFAFAVAALMSKRLDVDWLVAIRKWTLLSWLFLS
                     IGICLGMWWAYVELGWGGYWAWDPVENASFLPWLVMTAFLHSVMIQEKRGMLKKWNLG
                     LILGAWVLSIFGTFLTRSGVIQSVHSFTQSPVGAYFSWFLFIAVALLTILYVSRLPLL
                     RAESSLESLVSREASFLFNNLMLVGIAFTVLVLTVYPLLSEWFTGQKIVMKPPAFDFV
                     NVPLGLALLALTGIGPLIAWRRASIPNLKRQFAVPVVACIATALIILLLGMRQVWPII
                     TISLSAFVLGTVTQEFVRGVRARHRQYGEIYPIAFFHLMARNRRRYGGYVVHTGIVLL
                     FVAFTGMAFKVETEASLKPGQSVTLASPYGWTYTFKHLGVSQYDALNRQVTAGTVVVS
                     RNGKTIGTMVPEKRQHVDSFGNPTFEPSTAVAIRSDLREDLYVVLGGVVNGTETAVYR
                     FTINPLVWWVWYGGMIVALGGVIVMWPGGTVGSNRVATGYTAKLGATE"
BASE COUNT          689 a         1155 c         1153 g          511 t
ORIGIN      
        1 agcgccgccg gcacgacggc gagcaggttg ttgcgcatgg cctgaatctc cttacccaag
       61 taggaacggt tgctgtttcc caaactcgga gaggagacgg cgtgagcggt ctgatgttaa
      121 ggggtgacgg aggtcacgaa ctcacgggtc gcgctgacgg ctgatcgctg acggctgact
      181 tacgcggcga ctcggctccc gcaacgcccg caaaatgagg aatcccgctc gaggagcgtg
      241 ccacacttcg tgcaaaatcg gtcctgggcc tccgttccca gccggcgccc gcattcggag
      301 cagaacgctg cccccgcttc aggctggaga ccatgcacgg ggcacgtgag acccgagtcc
      361 gcggcccgtt tcgcgacgag gggaagaact gcagtggatg gcgcctgggg cgccgccgga
      421 cgctcggggg gtgccccgga ggagatggag ccgcggtccc cttcgcgaag ggcgttcacc
      481 gcctcgcggg tataccgcgc cttcagcgat tcgtagtctt cgagggagag cttcccggtg
      541 gcgcggtcga attcaatctc cgacagcgca cgcaaggcca ccgcccgggg actcaggtcg
      601 tcatcgggat cggcacccgc gtccaccaag ggtccgggca cgcggggacg aagaatcggg
      661 tcgatgacca cccacaccgc gagcgcggcg agcagtaccc ccgccaccag cccccacacg
      721 gatgtcagga ttcgaagcgc gagagctcgc gctgcaccaa ggccaggtct tccggggatg
      781 gcgccggccc ggctggagct tcgacggcgt gcacggcgcc gacccgccgg gcccaccggc
      841 ggatcagcca cgcgagcccg gtcgccgcga cagcgacgac cgcccacggg ccgaaatagc
      901 cgaagagggc gaatccgcgc ttcgggggtg acatcaagat cgattcgcca tgctccttga
      961 cgaactcggc gatgatgtcg ttcgcggaca tgcccgacgc tgccagtgcc aggatctggc
     1021 gatggtaacc cgggctgtag gtacagctga aatcggtggt ccggcaggtg tagatgtcga
     1081 gcccgcagcc gcaggtgcac gacagccgct tctcgatggc ctgaatgccc gcatcattgt
     1141 cgcgggccgt cacggggtcg agcgggcgtc cggctcgcgc cggatcggcc agcgtcccgg
     1201 tgggacccgc acccggcagg ggcgcctgcg cggtgtccac cgggcgctga atcccctgcc
     1261 ccaggagtcg cgcgggtaga tcccccgcga cgagtccccc cgcgagtgca ccccagaact
     1321 gccgtctcgt gagtcccgcg ttcattccgt tgctcccagc tttgccgtgt atccggtggc
     1381 gacccggttc gacccgaccg tcccgcccgg ccacatcacg atcacgccgc ccagggcgac
     1441 gatcatgccc ccgtaccaga cccaccatac caggggattg atggtgaacc ggtacaccgc
     1501 ggtctccgtc ccgttcacga cgccgcccag gacgacgtac aggtcctcgc gcaggtcact
     1561 gcgaatggcg acggcggtgg acggctcgaa cgtggggttg ccgaacgaat cgacgtgctg
     1621 gcgtttctcc ggcaccatcg tcccgatggt cttgccgttc cgggagacca ccacggtccc
     1681 cgccgtcacc tgccggttca gcgcgtcgta ctgggacacg cccagatgct tgaacgtgta
     1741 ggtccacccg taggggctgg cgagcgtcac cgattgtccc ggcttgaggg aggcctcggt
     1801 ttcgaccttg aacgccatgc ccgtgaacgc cacgaacagc agcacgatac cggtatggac
     1861 gacatagcct ccgtaccgac ggcgatttcg tgccatcaga tggaagaacg cgatcggata
     1921 gatctcacca tactggcggt gacgcgcgcg gaccccgcgg acgaactcct gggtcacggt
     1981 gccgaggacg aacgccgaca gggagattgt gatgatcggc cagacctgcc gcatgcccaa
     2041 cagcaggatg atgagtgcgg tcgcgatgca ggcgaccacc ggtacggcga actgccgctt
     2101 gaggttcgga atcgatgccc gccgccacgc aatgaggggc ccgatgcccg tgagcgcgag
     2161 cagcgccagg cccaggggca cgttcacgaa atcgaacgcc ggcggcttca tgacgatctt
     2221 ctggcccgtg aaccattcgg acaggagcgg atagaccgtc agcaccagga ccgtgaacgc
     2281 gatgccgacg agcatcagat tattgaaaag gaagctcgcc tcgcggctca ccagggactc
     2341 gagcgaggac tcggcccgga gcaacggcag acgtgagaca tacaaaatcg tgagcagggc
     2401 gacggcgatg aacaggaacc aggagaagta ggcgccgacc ggagactgcg tgaatgaatg
     2461 caccgactgt atgacgccgg accgggtgag gaacgtgccg aagatcgaca gcacccacgc
     2521 ccccaggatc aatcccaggt tccatttctt cagcatcccc cgcttttcct ggatcatgac
     2581 cgaatgcaga aatgcggtca ttacgagcca tggcaggaac gacgcgttct cgaccgggtc
     2641 ccaggcccag tacccccccc accccagctc cacgtatgcc caccacatcc ccaggcagat
     2701 cccgatggaa agaaacagcc aggagaggag cgtccacttc cggatcgcca ccagccaatc
     2761 cacgtccagc cgtttggaca tgagcgcggc gaccgcgaag gcgaatggaa tggtgatgga
     2821 gatgtagccg aggtagagca tcggcggatg gaagaccatc ccggggttct gcagctgcgg
     2881 gttcatcccc cggccgtcca ccggcgtata gggcaggcgc tcaaacgggt tggccgcgcc
     2941 cagcatgacg accaggaaga acaccgcgac gacgcacacc accgccgaca cgtacgggag
     3001 gtagacccgg tggcggggcg ccgtgagcac catcgccagg gacccgaaga tcgacagcac
     3061 ggtcgcccag aagagcaggc ttcccttctg tcccgcatac agggctgacc aggtgtagaa
     3121 ggtcgggagg ttccggctgg tgtaggcggc gacgtattcg acgttgaagt cacgggcgaa
     3181 caggagccat tcgacgcaga acaccgcgac caggagggtc aagcacatcg cgaacacggc
     3241 atgccgggag ctctgctgca gctcgatgcg cccggtcgag gcacccacga aacccgtcgc
     3301 cgcaccccag acgcccatca ggcaggcgat ccagagcgcg aggctcccga gaattgtcac
     3361 ggatcaggcc ttggggactg cttcgtaccg cgacccgcac ttggcgagta cgtcggtggc
     3421 gtggatgacg ccgtccttct gaaggcggcc ttccacgacg acctcgaccg aatcggtgaa
     3481 ggtgtcggga ggcaggccgt ggaagact
//
LOCUS       DASUBP010000027         7322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1267914, whole genome shotgun sequence.
ACCESSION   DASUBP010000027 DASUBP010000000
VERSION     DASUBP010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7322
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267914"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..208
                     /locus_tag="VFU75_01170"
     CDS             <1..208
                     /locus_tag="VFU75_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002379278.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEU5465469.1"
                     /translation="QRYKGLGEMNPDQLWKTTMDPAARTILKVTMEDALEASQLFETL
                     MGDEVEPRRKFIEENAKFVRNLDV"
     gene            286..492
                     /locus_tag="VFU75_01175"
     CDS             286..492
                     /locus_tag="VFU75_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465470.1"
                     /translation="MATAITVVILILAALAWLIFGGGDDPDTKPNPDIDRDELAEAES
                     EVRDAPDPDSVRDWGPGVPKDKPH"
     gene            505..2187
                     /gene="ettA"
                     /locus_tag="VFU75_01180"
     CDS             505..2187
                     /gene="ettA"
                     /locus_tag="VFU75_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HEU5465471.1"
                     /translation="MANPQYIYTMVGVGKVVPPSRQILKDIYLSFYPDAKIGILGSNG
                     AGKSSLLKIMAGIDHDHLGQAKLADGCTVGFLQQEPVLDPRKDVRGNVEEGLSHIRDL
                     LVQFNDLSAKFAEPMSDDAMAALLEKQGVLQEAIDHAGAWELDNKIDVAMDALRLPPG
                     DADVTKLSGGEKRRVALCRLLLTQPQLLLLDEPTNHLDAESVAWLEHHLHTYPGCVIA
                     VTHDRYFLDNVAGWILELDRGEGIPWKGNYSSWLEQKEKRLATEEKQASARQKTLERE
                     LEWIRMAPRARQAKSKARITAYEKLAAEEEQRRDDTAEILIPVPPRLGDDVVTAKDLT
                     KGFGDRLLFEKLNFTLPRGGIVGIIGPNGAGKTTLFRMMVGQEKPDGGSLTVGQTVQV
                     AYVDQSREALSPDRTVYEEISGGQDNLMFGKRSVNARAYCASFGFRGADQQKKVGVLS
                     GGERNRLHLAKLLKSGGNLLLLDEPTNDLDVDTLRALEDALLRFAGCVVVISHDRWFL
                     DRIATHILAFEDDGEVKWFEGNFQDYHADLVKRKGADADRPHRLKYRKLVRG"
     gene            complement(2184..3527)
                     /locus_tag="VFU75_01185"
     CDS             complement(2184..3527)
                     /locus_tag="VFU75_01185"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HEU5465472.1"
                     /translation="MFVVPLIERKRDGAALSAEEWSALIAAYTEGRVPDYQLSALLMA
                     VVFRGLERHELAALTDAMIASGDRLSFDGWNTPRIDKHSTGGVGDKVSLILAPLVAAC
                     GVAVPMMSGRGLGHTGGTLDKLEAIPGFKTGLSLAEAKAQVMKIGCALIGQTPEIAPA
                     DKRLYALRDVTATVEAIPLIAASIMSKKLAEGLTGLVLDVKHGSGAFLPPTEKGLELA
                     KTMIALGEDRGCPTVALLTAMDRPLGRACGNALETEEAILSLRGEGPEDLMEVTYALG
                     VEMLLQAKVETTAKKARKKLESVLGSGLAAEKFEQIIEAQGGNPKAVEDPSVMPQAGA
                     VEVFMAPATGVVQRVDPRIIGRAVVQLGGGRTKVEDTVDPTVGFVITVKPGDKVLAGE
                     PIASVFARDPAGIKLGFDALTQAIVVGDKLKEKPLPLISHRVTKTGVEVLGKAAS"
     gene            3556..4362
                     /locus_tag="VFU75_01190"
     CDS             3556..4362
                     /locus_tag="VFU75_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465473.1"
                     /translation="MRSALLLTLCLTGTLAAQETVASHIARGDSLRAALQSSAALAQY
                     QAALKVDSNAYEANWKAAREIADVAKQLLGDSLASRRDSLYSLGRVWAERAVQADSNG
                     ENGHFALALVLGRLSLTKSKKERVKYAKVIYDEAVHTLQLDSTHDGAEHILGQWNAEV
                     KRLSGLTRFFAKALLGGGFMDRANWDDAVKYLSLAVKHNPTHVYHRLALAAVYVDLDS
                     IPQATALLESIAALPVGDPMDPTYKKWAAEALADLKSGKKDDADDRLRHI"
     gene            4404..5192
                     /locus_tag="VFU75_01195"
     CDS             4404..5192
                     /locus_tag="VFU75_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5465474.1"
                     /translation="MTISSALTHAEGERLAARIAELEHERKHLLAVIEILKEVSGTLH
                     FTDILQSIARKLGEAFGLDRCSIFLAERGGKSVRLVASYEDPTIRNYVVDLERYPELK
                     RAIQGAETVFIPDAANDPSLKHVRADLVNRRVKSITVVPINWRGVAIGAIFLRTFRDG
                     PTFSDSDVRFVQVVASLTAQSLRNAHRYERLAQRHRDPESREARHIELEGVALLSFLR
                     RLMEAVGQRQGAWAEGALPRASAEELDRLVGVALAVIQEEAKGR"
     gene            5189..7198
                     /gene="ligA"
                     /locus_tag="VFU75_01200"
     CDS             5189..7198
                     /gene="ligA"
                     /locus_tag="VFU75_01200"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEU5465475.1"
                     /translation="MTDPAARAATLRQIVERAARAYYVGDRPEMSDAEYDRLFRELQA
                     LEAQHPELQTPDSPTQRIGAEPSSAFRKHRHLVPMLSLDNAFDPAELTAWEERNARLA
                     PEVTKAGYTLEVKIDGAAVCLTYRKGLLVTGATRGNGVEGEEITPNLKTIRDVPLKLS
                     GSGWPELMEVRGEVYFENSRFKALNAEREAAGEPTFANPRNSAAGSLRQLDPKITRSR
                     KLRMFCFHVEAPGSVLPFDTQEQLLLTLVQWGFPVEPHHRTVPTLAAAQEAIAQLEAL
                     LPTLDYGADGVVVKVNQRRLHDELGVVGDREPRWAIARKFAPEVQITKLIDIGINVGR
                     TGALNPFAMLEPVDIGGATVSNATLHNADLIAARDIRIGDWVEVTRAGEVIPQVLGPV
                     RERRTGSEVPFQMPEKCPRCGSEVEHPQDEVMSFCPNVSCPGRVLESIVHFAMAMDIR
                     GLGYERVRAFLDAGLIQDVADLYRLTMDQLRGLEGFAAKSAKLLVDAIAATKTQPLSV
                     VLYALGIRHVGAQGAKLLARQFGTMEALEEAPQDVIGSIRGIGPAIAEAVWHFFRERR
                     NQNLIAHLKAAGLTLAEPESRAAEGAPLAGQTYVVTGTLPTLSRKAATDLITGAGGHV
                     GSSVSKQTTAVVAGADAGSKLDRAKTLGIPVIDEAELLRRIQSNL"
BASE COUNT         1276 a         2552 c         2318 g         1176 t
ORIGIN      
        1 ccagcgctac aaggggctcg gcgagatgaa ccccgatcag ctctggaaga ccacgatgga
       61 tccggccgcg cgcaccatcc tcaaggtcac gatggaagac gcgctcgaag cttcccagct
      121 cttcgaaaca ctgatgggcg acgaggtgga gccccgtcgg aagttcatcg aggagaatgc
      181 gaagttcgtc aggaatctgg atgtgtgatg tgtgagggat cagggatcag gggtcagggg
      241 tcagacccac tggtccctca cccctgatcc ctggcacctt tctccatggc caccgccatc
      301 accgtcgtca tcctgattct cgctgccctc gcctggctga tcttcggggg cggcgacgat
      361 ccggacacca agcccaatcc cgacatcgac cgggatgagc tggcggaagc cgaatcggaa
      421 gtgcgtgacg cccccgatcc tgacagcgtc cgcgattggg gtcccggcgt tccgaaggac
      481 aagccacact aacactcgag tcccatggcg aatccgcagt acatctacac gatggtgggg
      541 gtgggcaaag tcgtaccccc ctcccgccag atcctcaagg acatctacct ctcgttctac
      601 ccggacgcca agatcggcat cctggggtcg aacggcgccg gaaagagctc gctcctcaag
      661 atcatggccg ggatcgacca cgatcacctc ggccaggcca aactcgccga cggctgcacc
      721 gtgggcttcc tccagcagga gcccgtcctc gatcccagga aggacgtccg cgggaacgtg
      781 gaggaggggc tctcccacat ccgcgacctc ctggtccaat tcaacgacct ctccgccaag
      841 ttcgcggagc cgatgtccga tgacgccatg gcggccctgc tcgaaaagca gggcgttttg
      901 caagaagcga tcgaccacgc cggcgcgtgg gagctcgaca acaagatcga cgtcgccatg
      961 gacgccctgc gcctgccccc cggcgacgcc gacgtcacca agctctcggg cggcgaaaag
     1021 cgccgcgtcg cgctgtgccg gctcctgctc acccagcccc agctgctcct gctcgacgaa
     1081 cccactaacc acctcgacgc ggaatcggtg gcctggctgg agcaccatct ccacacctat
     1141 cccggctgcg tcatcgccgt cacgcacgac cgctatttcc tcgacaacgt cgccggctgg
     1201 attctcgagc tggaccgggg cgaaggcatc ccgtggaagg gcaactactc atcgtggctc
     1261 gagcagaagg agaaacggct cgccaccgaa gagaagcagg cgtcggcccg gcagaagacc
     1321 ctggagcgtg agctcgagtg gatccggatg gcgccgcgcg cccgtcaggc gaagtcgaag
     1381 gcgcgcatca ccgcctacga gaagctcgcg gcggaggagg aacagcgccg ggacgacacg
     1441 gccgaaattc tcattcccgt gccgccccgt ctcggcgacg acgtcgtcac cgcgaaggat
     1501 ctgaccaagg gcttcggcga ccgcctgctc ttcgagaaac tgaacttcac cctgccccgc
     1561 ggcgggatcg tcggcatcat cggtccgaat ggcgcgggca agaccacgct gttccggatg
     1621 atggtgggcc aggagaagcc cgacggcggg tcactgaccg tcggccagac cgtgcaggtc
     1681 gcctacgtgg accagagccg ggaggccttg agccccgacc ggacggtcta cgaggagatt
     1741 tccgggggac aggacaacct gatgttcggg aagcgctccg tgaacgcgcg cgcctactgc
     1801 gcgtctttcg gattccgcgg cgccgatcag cagaagaagg tgggcgtcct ctcgggcggc
     1861 gagcggaacc gcctgcatct cgccaagctc ctgaagagtg gcggcaatct gctgctcctg
     1921 gacgagccga ccaacgacct cgatgtcgat accctccgcg cgctcgagga cgccctgctc
     1981 cgcttcgccg ggtgtgtggt ggtgatcagc cacgaccgct ggttcctcga ccggatcgcg
     2041 acccatatcc tggccttcga agatgacggt gaggtgaagt ggttcgaggg gaacttccag
     2101 gactaccacg ccgatctcgt gaagagaaag ggggccgacg ccgatcggcc ccaccggctc
     2161 aagtatcgga agctggtccg cggctaggaa gcggctttcc cgagcacctc cacgccggtc
     2221 ttggtcaccc ggtgggagat gaggggcaac ggcttttcct tgagcttgtc gccgaccacg
     2281 atggcttgcg tcagcgcatc gaatccgagc ttgatcccgg cggggtctcg cgcgaacacg
     2341 gaagcgatag gttccccggc cagcaccttg tctcccggct tcacggtgat cacgaagccg
     2401 acggtgggat ccacggtgtc ctcgaccttg gttctgccgc ctcccaactg caccaccgcg
     2461 cgcccaatga tgcggggatc tacccgctgt actacgccgg tggccggggc catgaagacc
     2521 tcgacggccc ccgcctgcgg catcaccgag ggatcctcga cggccttggg gttcccgccc
     2581 tgggcttcga tgatttgctc gaatttttcc gccgcgaggc cggaccccaa caccgactcc
     2641 agcttcttgc gggccttttt cgccgtggtc tcgaccttcg cctgcagcag catctccacg
     2701 ccgagcgcgt aggtcacttc catgaggtct tccgggccct cgccgcgcag cgagaggatg
     2761 gcctcctcgg tctcgagcgc gttcccgcac gcccggccca ggggccggtc catcgccgtg
     2821 agcaacgcga cggtgggaca cccgcggtct tcacccagcg cgatcatggt cttcgcgagc
     2881 tccaggcctt tctccgtcgg aggcaggaag gctccactgc cgtgcttcac gtcgagcacc
     2941 aggccggtca gaccctcggc cagcttcttc gacatgatgc tcgcggcgat cagcgggatg
     3001 gcttcgaccg tcgcggtgac gtcgcggagg gcgtagagcc gcttgtccgc cggcgcgatc
     3061 tccggggtct ggccgatgag cgcgcacccg atcttcatca cctgcgcctt ggcttcggca
     3121 agggagagac cggtcttgaa cccgggaatc gcttcgagct tgtcgagggt gccgccggta
     3181 tgccccaggc cgcggcccga catcatcggg accgccacac cgcacgcggc caccaggggc
     3241 gccaggatca gcgacacctt gtccccgacc ccgcccgtag aatgcttgtc gattcgcggc
     3301 gtgttccagc cgtcgaacga gagtcggtcc ccggaggcga tcatcgcgtc cgtgagcgcg
     3361 gccagctcgt ggcgctcgag accccgaaag accacggcca tgagcagcgc tgagagctga
     3421 tagtccggaa cgcgcccttc ggtgtatgcg gcaataaggg cggaccattc ttccgccgac
     3481 agggcggcgc cgtcgcgctt gcgttcaatg agaggtacga cgaacattga ccccttcctc
     3541 cgccggagac cggtgatgcg ctcagccctg ctcctgaccc tgtgcttgac cggaacactg
     3601 gctgcccagg aaaccgtcgc cagtcacatc gcccggggag attcgctccg ggccgccctg
     3661 cagtcttccg ccgccctcgc gcagtaccag gccgccctga aggtggactc gaacgcctac
     3721 gaagccaact ggaaagccgc ccgggaaatc gccgacgtcg cgaagcagct cctcggtgac
     3781 tccctcgcct cccgccgcga ctccctgtac tccctcggcc gcgtctgggc cgagcgggcc
     3841 gtgcaggccg actccaacgg tgagaacggg cacttcgcgc tcgccctggt actgggccgg
     3901 ctgtcgctga ccaagagcaa gaaggagcgg gtgaagtacg ccaaggtcat ctacgacgag
     3961 gcggtgcaca cgctgcagct cgattccacc cacgacggcg cggaacacat tctgggtcag
     4021 tggaacgcgg aagtgaaacg actctccggc ctcacccggt tcttcgccaa ggccctgctc
     4081 ggcggcggtt tcatggaccg ggcaaactgg gacgacgcgg tgaagtatct ctctctcgcc
     4141 gtgaagcaca atcccaccca tgtgtaccac cggctggccc tcgccgcggt ctatgtggat
     4201 ctggattcca ttccacaggc caccgccctg ctcgagtcga ttgccgcgct cccggtcggg
     4261 gatccgatgg acccgaccta caagaagtgg gcggccgagg ccctcgcgga cttgaagagc
     4321 ggcaagaaag acgacgccga cgaccgcttg cgtcacattt aagtccttga cggaagcggg
     4381 gttgcgaccc caaactattt gccatgacca tctcctcggc actcacccac gcagagggcg
     4441 agcgccttgc cgcgcggatc gccgagctcg agcacgagcg caagcatctc ctcgccgtga
     4501 tcgagatcct caaggaagtc tccggcacgc tccacttcac cgacatcctg cagtccatcg
     4561 cccgcaagct gggcgaggcg ttcggtctcg accggtgctc gatttttctg gccgaacgcg
     4621 gtggaaaatc ggtccggctc gtcgcttcgt acgaagaccc caccatccgc aactatgtgg
     4681 tggacctcga gcgctatcct gagttgaagc gcgccatcca gggcgccgag acggtgttca
     4741 tcccggatgc cgcgaacgat ccgtccctga agcacgtgcg cgccgacctg gtgaaccgcc
     4801 gggtgaagtc gattacggtg gtgccgatca attggcgggg cgtggccatc ggcgccattt
     4861 tcctccgcac cttccgtgac ggcccgacct tctcggactc ggatgtccgt ttcgtccagg
     4921 tcgtagcctc tctgacggcg cagtccctcc gcaacgccca tcgctacgag cggctggccc
     4981 agcggcatcg ggaccccgag agcagggaag cgcgtcacat cgagctcgaa ggggtggccc
     5041 tgctctcctt cctgcggcgc ctgatggagg cggtcggcca gcggcagggc gcctgggccg
     5101 aaggcgccct gccccgggcg tcggcggaag agctggaccg cctggtcggc gtggcgctcg
     5161 ccgtcattca ggaagaagcc aaaggccggt gacggacccg gcggcccgcg ccgccacttt
     5221 gcggcaaatc gtcgagcggg cggcccgcgc gtactacgtc ggcgaccgcc ccgagatgtc
     5281 cgacgccgag tacgaccggt tgttccgtga gctgcaggcc ctcgaagccc aacaccccga
     5341 gctccagact cccgactctc cgacccaacg gatcggcgcg gagccctcca gcgccttccg
     5401 gaagcaccgc cacctggtcc ccatgctctc cctggacaac gcgttcgatc cggcggagct
     5461 caccgcctgg gaagagcgga acgcgcgtct ggcacccgag gtcaccaagg ccggctacac
     5521 cctggaggtg aagatcgacg gcgccgccgt ctgtctcacc taccggaaag gcctgctcgt
     5581 caccggagcc acgcgtggga acggcgtcga aggcgaagag atcacaccga acctcaagac
     5641 cattcgtgac gtccctctca agctgtcggg ctcggggtgg ccggagctca tggaagtccg
     5701 cggcgaggtc tacttcgaga attcccgttt caaggccctg aacgcggagc gcgaggccgc
     5761 cggcgaaccg actttcgcca atccccgcaa ttcggccgcg ggctcactcc gtcagctgga
     5821 ccccaagatc acgcggtccc ggaagctccg gatgttctgc ttccacgtcg aggcgccggg
     5881 gagcgtcctt ccgttcgaca cgcaggagca gctgctcctc accctggtgc aatggggatt
     5941 cccggtcgag cctcatcacc ggacggtgcc gacgttggcc gcggcccagg aagcgatcgc
     6001 ccaactcgaa gcgctcctcc ccaccctcga ctacggtgcc gacggcgtcg tcgtgaaggt
     6061 gaaccagcgg cgtctgcacg acgagctggg cgtggtcggg gatcgcgagc cccgctgggc
     6121 catcgcgcgg aagttcgcgc ccgaggtgca gatcacgaaa ctgatcgaca tcgggatcaa
     6181 cgtcggacgg acgggagcgc tcaatccctt cgccatgctc gaacccgtag acatcggcgg
     6241 tgccaccgtc tccaacgcta cgctccataa cgccgacctg atcgcggcgc gggacatccg
     6301 gatcggcgac tgggtggaag tcacccgcgc cggcgaggtc attccccagg tcctcggccc
     6361 cgtccgcgag cggcgcaccg gcagtgaagt ccccttccag atgcccgaga agtgcccgcg
     6421 ctgcgggtcg gaggtggaac atccgcagga cgaagtgatg tcgttctgcc ccaacgtctc
     6481 ctgccccggc cgggtgctcg aatcgatcgt ccatttcgcc atggcgatgg acatccgcgg
     6541 gctgggctac gagcgggtcc gggccttcct ggacgccgga ctgatccagg atgtcgcgga
     6601 cctctaccgg ctcacgatgg accagctgcg gggcctggag ggcttcgccg cgaaatcggc
     6661 gaagctcctg gtggacgcca tcgccgcgac gaagacccag cccctttcgg tagtactcta
     6721 cgccctcggg atccggcacg tcggcgctca gggagccaag cttctggcgc ggcagttcgg
     6781 caccatggag gcgctcgagg aggcgcccca ggacgtcatc ggctccatcc gcgggattgg
     6841 tcccgccatc gccgaggcgg tctggcactt cttccgggag cggcgcaacc agaacctgat
     6901 cgcccatctg aaggccgccg gcctcaccct ggccgagccg gagtcccgcg ccgcggaggg
     6961 ggcgcccctg gcggggcaga cctacgtcgt caccggcacg ctccccacgc tctcccggaa
     7021 ggcggcaacg gacctcatca ccggtgccgg cggccatgtc ggcagttccg tgtccaagca
     7081 gacgacggcg gtcgttgcgg gagcggacgc cgggtcgaag ctcgaccggg ccaaaacgct
     7141 cggtattccg gtgatcgacg aagccgaact gttgcgccgc atccagtcaa acctgtaagt
     7201 tgggctccca gatatgagcc cttcctccgc ctccgcccgc accgacggcc ttgccatcgg
     7261 gcgggcgtct ttgcaacaac tgcatcggtc cctgcttcag cacgcgcccg accaggcggt
     7321 ga
//
LOCUS       DASUBP010000028         4731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_682049, whole genome shotgun sequence.
ACCESSION   DASUBP010000028 DASUBP010000000
VERSION     DASUBP010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_682049"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..270)
                     /gene="ssrA"
                     /locus_tag="VFU75_01205"
     tmRNA           complement(<1..270)
                     /gene="ssrA"
                     /locus_tag="VFU75_01205"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(283..1797)
                     /locus_tag="VFU75_01210"
     CDS             complement(283..1797)
                     /locus_tag="VFU75_01210"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEU5465476.1"
                     /translation="MNQKVRWLGRGLVVLGLAVVVFSILHAFHWALISGVGGTLVGIG
                     MLLSAAERGWRSESGVTGAPIADFDHLTDLVRRAHQARGSWIVGTREGDLTAGVADLE
                     DHERDRGYALAQLAAVNERPHVSREGDGTYVAVGAFPYGAAVLLGPGKGGVVATDAVT
                     EDLRRLVASLAQVEADWAQGDRVARQLAALTAPGQTLEAVATAAARLTEQISGQGAVV
                     VIQESSTRALRVRGVSPHADPRLLFLVVPEDSPAGRAVATELHVVGGPGEDVLGPGMP
                     ERRQRDRMGTAFPLMDGGHSIGALVVIGAPLDAGSPKAEQVNRLVAELGSRLGAVRAV
                     NEAQERAVKDALTGLHNRREFDRRFENYKVEGLSRPAALIYLDLDHFKRLNDSLGHPA
                     GDAALVHVARILERETRGADLVARIGGEEFAVWLPETTLSKAMDIAERMRRAIENGGW
                     RWDGQEQRLTVSSGVSACPDSSSSLENLRALADAALYRAKEAGRNRVEKATPSK"
     gene            complement(1794..2480)
                     /gene="larB"
                     /locus_tag="VFU75_01215"
     CDS             complement(1794..2480)
                     /gene="larB"
                     /locus_tag="VFU75_01215"
                     /inference="COORDINATES: protein motif:HMM:NF033503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HEU5465477.1"
                     /translation="MNLKDVDGLIERLAFASIDHLRPQTQGHQEVILCQGKTPEQTVA
                     IAERLAERSGSFLATRAEPAHRDGLIARFPKAEVNELGRVVYLAPDPEPAPTGRGTVL
                     IITAGTGDLPVAEESAVTARAFGNKVERLTDVGVAGIHRILTQTELLRNASVIIVVAG
                     MEGALPSVVGGLVKCPVIAVPTSIGYGASFGGVAALLGMLNSCASGVTVVNIDNGFGA
                     AAAASRINHT"
     gene            complement(2477..3493)
                     /gene="dusB"
                     /locus_tag="VFU75_01220"
     CDS             complement(2477..3493)
                     /gene="dusB"
                     /locus_tag="VFU75_01220"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEU5465478.1"
                     /translation="MQPPITLQDMDFPYPTGHAVPLYLAPMAGVSEGPFRQICRRFGA
                     DVVVSEFVSSVGISLGIERVLADMRFEPVERPIGIQLYGADAGVMARAAGMVTELFHP
                     DFIDINFGCPVKKVVRNNGGSGCLKDMSLVGSIIKAVKGATHLPVTVKIRSGWDEAQR
                     DPVSIALRCQEAGALALALHARTRTQMYTGEANWDEIAHVVDALDIPVIGNGDIETAE
                     DILAMHRHTNCAGIMIARGTFGNPWIFERGHALLEGRVPPPVPTAEERFAVALDHAQL
                     ALRLQGDTRKTALEFRKHLGWYTKGLRGAAELRQRLFAIESMAEAERLFAAYLEESEV
                     EAAA"
     gene            3565..4017
                     /locus_tag="VFU75_01225"
     CDS             3565..4017
                     /locus_tag="VFU75_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEU5465479.1"
                     /translation="MHLREFFAEDAINLHLASADKDDVLKELVGLLHLEEKEDAMLFK
                     MLKRRETLGSTGIGQNIAIPHCRSLVVSKLRVAFGRKLEGVDFKAIDDKPVHYFFLIV
                     APPLEVSNQYLPVLGKIAQFAKEPDVPERLAKLNTPAEFLQLLEEKKI"
     gene            complement(4110..>4731)
                     /locus_tag="VFU75_01230"
     CDS             complement(4110..>4731)
                     /locus_tag="VFU75_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003771338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GMP synthase (glutamine-hydrolyzing)"
                     /protein_id="HEU5465480.1"
                     /translation="DAAKHVSGDVGFLVQGTLYPDVIESSSPKGGPSVTIKTHHNVGG
                     LRPDLPWKLVEPLRELFKDEVRQVGRELGLPEEIVGRHPFPGPGLAIRVLGPVSEERL
                     AILRKVDAIYIEEIRAAGLYDQIWQAFAVLLPVKSVGVMGDGRTYDHVVALRAVTSRD
                     GMTADWFPFPYDTLARISSRVINEVRGVNRVAYDVSSKPPATIEWE"
BASE COUNT          820 a         1690 c         1458 g          763 t
ORIGIN      
        1 gcccaggcgc ctcgccggcc caagcagcga caagcctcta ccagctctta cgcgatctct
       61 tgaggcccga aatcacgcca gcccgacttt gcgatacgtg agcgccgcct caggcgggct
      121 gccactcacg caagccggta agccgaagct tacgcagcca gtgccaggtt tgaattggca
      181 gttgtgaatt tcccagttga ttatccaggt gactgagaac ctgggcacgc agcgacaccc
      241 tcacagaacc cgtcgaaacc tgtcgccccc gactacccga acctatttgg acggcgtcgc
      301 cttttcaacc cggttgcgcc ctgcctcttt ggcacggtat aacgccgcat ccgccaatgc
      361 ccgaaggttc tcgagactcg acgagctgtc cggacaggcc gagacgccgc tcgaaacggt
      421 cagacgctgc tcctgcccat cccaccgcca cccgccattc tcgatcgccc tccgcatgcg
      481 ctcggcgata tccatggcct tcgacaacgt cgtctccggc agccacaccg cgaactcctc
      541 gccgccgatc ctggcgacca ggtcggcgcc gcgcgtctcc cgctcgagga tccgcgccac
      601 gtgcaccagc gccgcgtctc cggcgggatg cccgagtgaa tcgttgagcc gcttgaagtg
      661 gtcgaggtcg aggtagatca aggcggccgg gcggctcagg ccctcgacct tgtagttttc
      721 gaaccgccgg tcgaactccc gccggttatg cagcccggtg agggcgtcct tcaccgcccg
      781 ctcctgggcc tcgttcaccg cccgcaccgc gcccagccgc gaccccaact ccgccaccag
      841 acggttcacc tgctcagcct tggggcttcc ggcatcgagg ggagcgccga tcaccaccag
      901 ggctccgatc gaatgaccgc cgtccatcag cgggaaggcc gtacccatgc ggtcgcgctg
      961 gcgccgctcc ggcatcccgg gccccagaac atcttcacca gggccgccca cgacgtggag
     1021 ctccgtggcg acggctcggc ccgcgggaga gtcctcagga accaccagga agagcaaccg
     1081 ggggtcggca tgaggagaca cgccccgcac gcgaagggcc cgcgtggagg actcctggat
     1141 cacgacgaca gctccctgcc ccgaaatctg ctccgtgagc cgggcggcag ccgtcgccac
     1201 cgcctccaac gtctgacccg gtgcggtgag cgccgcgagc tgccgcgcga cccggtctcc
     1261 ctgggcccag tcagcctcga cctgcgcgag ggaggccacg aggcggcgca ggtcttccgt
     1321 gaccgcgtcg gtggcgacca caccgccctt ccccggcccc agcaacaccg ccgccccata
     1381 cgggaaggct cccaccgcga cgtaggttcc atcgccttcg cgtgacacgt gcggccgttc
     1441 gttcaccgcc gcaagctgtg ccagcgcgta cccacggtcc cgctcatggt cttcgagatc
     1501 cgcgacgccc gccgtcagat ccccttcccg cgttcccacg atccaggacc cacgcgcctg
     1561 atgtgctctc cgcacgaggt ccgtgaggtg gtcgaagtcc gcgatgggtg cgccggtgac
     1621 ccccgactcg ctccgccagc cccgctccgc ggcggacaag agcatcccga taccgacgag
     1681 ggtgcccccg accccgctga tgagggccca gtggaacgcg tgaagaatgg agaagacgac
     1741 gaccgccaac ccgagcacga ccaacccgcg cccgagccag cggaccttct gattcacgtg
     1801 tggttgatgc ggctcgccgc ggccgccgcg ccgaagccgt tgtcgatgtt gaccacggtg
     1861 acacccgagg cgcaggagtt caacatgccg aggagcgcgg cgaccccgcc gaaggacgcg
     1921 ccatagccga tgctggtggg gaccgcgatg accgggcact tcacgagacc gccgacgacc
     1981 gagggcagcg ccccctccat gccggcgacc acgatgatga ccgaggcgtt ccgaagcaat
     2041 tccgtctgag tcaggatgcg atggatgccg gcgacaccga cgtcggtcag gcgttcgacc
     2101 ttgttcccga atgcccgggc ggtgaccgcg gattcctcgg ccaccggcag atctccggtc
     2161 cccgcagtga tgatgagtac ggtgcctcgc ccggtgggtg ccggctcagg atcgggcgcg
     2221 agatagacca cccgccccag ctcgttcact tcagctttgg ggaaccgcgc gatcaacccg
     2281 tcccggtgtg caggctccgc ccgggtcgcc aggaagcttc cgcttctctc ggccaaccgc
     2341 tcggcgatcg cgaccgtctg ctcgggagtc ttcccctgac acagaatcac ctcctgatgc
     2401 ccctgagtct ggggccggag gtgatcgatc gaggcgaagg ccaggcgctc gatgaggccg
     2461 tctacgtctt tcaggttcac gccgccgcct cgacctccga ctcctccaag tatgccgcga
     2521 acagccgctc ggcctccgcc atcgattcga tcgcgaagag ccgctggcgc agctcggccg
     2581 cgccccggag ccccttggtg taccacccca ggtgcttccg gaactcgagc gccgtcttcc
     2641 gggtgtcccc ttggagacgc agggcgagct gggcgtgatc gagcgccacg gcaaaccgct
     2701 cttcggcggt gggcaccgga gggggcaccc ggccttccag cagtgcgtgg ccgcgttcga
     2761 agatccacgg attgccgaac gtcccgcgag cgatcatgat gccggcgcag ttcgtgtgcc
     2821 ggtgcatcgc gaggatgtct tcggccgttt cgatgtcccc gttgccgatt accggaatgt
     2881 ccagcgcgtc gaccacgtga gcgatctcgt cccagttggc ctctcctgtg tacatctggg
     2941 tccgcgtccg ggcgtgcagg gcgagcgcga gggcgccggc ctcctgacag cgaagcgcga
     3001 tgctcacagg gtcacgctgt gcttcgtccc agccgctccg gatcttcacc gtcacgggca
     3061 ggtgcgtcgc gcccttcacg gccttgatga tgctgcccac cagcgacatg tccttcaggc
     3121 acccggaccc gccgttgttg cggaccactt tcttgacggg acaaccgaag ttgatgtcga
     3181 tgaagtcagg gtgaaacagc tccgtcacca tccccgcggc gcgcgccatg accccggcat
     3241 cggcaccgta cagctggatg ccgatcggcc gctccaccgg ctcgaaccgc atgtcggcca
     3301 gcacccgctc gatccccagg gaaatcccga ccgacgagac gaactccgac acgacgacgt
     3361 ccgccccgaa gcgccggcag atttgccgga acggtccttc ggagacgcct gccatcggcg
     3421 ccaggtagag gggcacggcg tgcccggtcg ggtacgggaa gtccatgtcc tgcaacgtaa
     3481 tgggaggttg catggcgagc aactcgcggc tacgttccgg tcccgacatc actgattcgt
     3541 gcttgaaaac ggagtgacgg ccccgtgcat ctgcgtgagt ttttcgccga agacgccatc
     3601 aatctgcatc tcgccagcgc cgacaaggat gatgttctga aggagctggt cggacttctc
     3661 catctcgagg agaaggaaga cgccatgctc ttcaagatgc tgaagcggcg cgagacgctg
     3721 ggatcgaccg gcatcggcca gaacatcgcc attccccact gccggtcact ggtggtgtcg
     3781 aagctgcgcg tcgcgttcgg aaggaagctc gaaggggtgg acttcaaggc catcgatgac
     3841 aagccggtgc actacttctt cctgatcgtc gctcctccgc tcgaagtctc caaccagtac
     3901 ttgcccgtgc tcggcaagat cgcccagttc gcgaaggagc cggatgtccc cgaaagactc
     3961 gcgaagctca acacgccggc ggagtttctg cagctgctcg aagaaaagaa gatctgaagt
     4021 gacagggagc ggggaggagg gagggggcga agtcaagcac gtggagagtc ctcaatccga
     4081 gactgcgacc cctccctctt ccctcctccc tattcccatt caatcgtcgc cgggggtttc
     4141 gagctgacgt cgtaggccac ccggttcacg cctcgcacct cgttgatcac ccggctcgag
     4201 atacgcgcca gcgtgtcgta cgggaacggg aaccagtcgg cggtcatgcc gtcccggctg
     4261 gtgacggcgc gcaacgccac gacgtgatcg taggtccggc catcccccat tacaccgacc
     4321 gacttcaccg gcagcaacac cgcgaacgct tgccagatct ggtcgtagag gcccgccgcg
     4381 cgaatctctt cgatgtaaat cgcgtccact ttccggagga tggccagccg ctcctcggag
     4441 acggggccca gcacgcggat ggcgagacca ggacccggga acgggtgccg gccgacgatc
     4501 tcctcaggca gccccagctc gcggccgacc tgccggacct catccttgaa gagctcccgc
     4561 aacggctcga ccagcttcca ggggagatcc ggccgcaggc cgccgacgtt gtggtgcgtc
     4621 ttgatcgtga ccgacggacc acccttcgga gaagacgact cgatgacgtc cggatagagg
     4681 gtcccttgca ccaggaatcc gacgtctccc gacacgtgct tcgccgcgtc c
//
LOCUS       DASUBP010000029        25268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1131754, whole genome shotgun sequence.
ACCESSION   DASUBP010000029 DASUBP010000000
VERSION     DASUBP010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 25268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25268
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131754"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="VFU75_01235"
     CDS             <1..1152
                     /locus_tag="VFU75_01235"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5465481.1"
                     /translation="VAFLIAVVAIIQGMNAYVRDRIGGAIVGVNAFQVRRTPLAGGLI
                     DDEEWKAIQRRPIIAPEDVPIVERAIPDAFAVGLQSGWPTPQADVVWGDKTVGDVQMF
                     GVNASYQVVQDYTFAAGDPLTDVDDRERRPVIELGWDISDKLFGDPDFAVGKKLRIMG
                     QQFTVKGVVAKKGTVLGISQDGFVMMPYSTFESLYGRRKTTVISVKMTDAAAVQPAMQ
                     RAEEAMRIAHRLRPSQADDFSVSTSDAFVTFWENLTRVLFTVVPVVVAIGIVVGGIVI
                     MNIMLMSVNERTREIGIRKSMGATRRDILNQFLAESVTLALLGGLLGLLAGGGLATLV
                     ELVTPLPARVTLWSVFLALGLGAGVGVVFGVYPATRAARLDPINALRAE"
     gene            1149..2390
                     /locus_tag="VFU75_01240"
     CDS             1149..2390
                     /locus_tag="VFU75_01240"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5465482.1"
                     /translation="MRLHSVSEGWQIAVDQLRGNKLRSGLTILGVVIGVATVMGMASI
                     VQGIRDQIINTIEVAGPTTFYVMRFFSQTPLNPDQLPKEVRIRPVLKPAEAKLVAGLP
                     GIGYAGMWVQLFNKMEFQGERTQLMTEFGADSHYMEIQGGELLEGRFFTSSEEASGAP
                     VVVLERAPADKLFGRIDPIGRFVRVGGNSLEVVGIYQNPANIFQPPSQQIGAIMPFET
                     ARRFFRYDQTNALWIVVKPVGGVTVGEAEDEVTTALRRSRGLGPGAPNSFDLLTQDQI
                     LNIFNSFTSTFFLVMVALSSVALMVGGIGVMAIMMVSVTDRTREIGVRKALGATRVEI
                     LWQFLIEASTLTLLGGLVGILIGLVFGQVIKLVLHFDSGVPVWSALVACLVSVLIGLI
                     FGVLPAQRAARLDPVEALRYE"
     gene            2465..3193
                     /gene="bshB1"
                     /locus_tag="VFU75_01245"
     CDS             2465..3193
                     /gene="bshB1"
                     /locus_tag="VFU75_01245"
                     /inference="COORDINATES: protein motif:HMM:TIGR04001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB1"
                     /protein_id="HEU5465483.1"
                     /translation="MTDTIETVDLLAIAAHRDDVELTCGGTLARTAQKARVGIVDLTQ
                     GEMGTRGDAAIRAAEAERAARVLGVHVRLNAGLPDAHIYNDDPSRKTVIELIRRTRPR
                     VVILPFPIGRHPDHRIASELARDACYLAGLKKYPASGEPHRPHKILYALAYREDTVKP
                     SFVVDISDVFETKMKAIRCYASQFDGAQAAGEIYPTGQHLYDLIQVQSAHYGSLIRRP
                     YGEPYFTQETVAVDDVLSLGVQSL"
     gene            3210..4394
                     /locus_tag="VFU75_01250"
     CDS             3210..4394
                     /locus_tag="VFU75_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HEU5465484.1"
                     /translation="MTAAKRSVYLDHAATTPVRPEVLEAMLPYLGVEAFGNPSSAHRV
                     GRAARAGVEEAKRRVAEALHVEPNQVIFTSGGTEADNLAIIGSALAAKDHGGHFRVAV
                     SATEHKAVLAAAHAVKHLGGDEIILPVDSDGLVDERAVEDALRRGVAIVSVMWVNNEV
                     GTVQPIAKLAARCREAGVAFHSDAVQAFGKVPVDLAEVPVTFLTISGHKIGAPKGIGA
                     LIVRDRHALEAIIHGGGQQFGIRPGTENVPGIVALGRAAELAAKEQATTAARVAKLRD
                     DLEARFLAVVEGAVVNGRNAPRAPQTVSFSIPGADGEALLMHLDLAGIACSGGSACST
                     GSVDPSHVLTAMGIARDMGSAALRFSLGRESTTEDVDALIEAMPKVVAKVRALTGALG
                     RA"
     gene            4387..5502
                     /gene="mnmA"
                     /locus_tag="VFU75_01255"
     CDS             4387..5502
                     /gene="mnmA"
                     /locus_tag="VFU75_01255"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEU5465485.1"
                     /translation="MPERVLVAMSGGVDSSVAAALLVEQGYEVIGATLKLFCYGDEVA
                     DRPCCSLDSITDAKLVANRLGIPHYVLNLEDRFGRDVIGNFVSEYAAGRTPIPCVRCN
                     SFTKFRDLLLHADALDCRYLATGHYAVARSGELHRGRDRSKDQTYFLWGIDRSVVARM
                     LTPVGALTKAETRARARELGLVTAEKPESVEICFVPDDDYVKVLERRLPADAPALSPG
                     DFVTRDGTVLGAHAGFARFTVGQRRGLPGGFPVPMFVTAIRPETREVVIGPADELLGH
                     QVILEEVNWIAEPLAADARVWVQCRYRAAAVPAVVLTTQGDHLTLALEEPVRAITPGQ
                     SGALYTREGRLLGGGVIALAATRCATSPSRRLAVLNS"
     gene            5544..6650
                     /locus_tag="VFU75_01260"
     CDS             5544..6650
                     /locus_tag="VFU75_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465486.1"
                     /translation="MSPIRKRIAGAAVLLLAAAACSKDATAPKPLDPTAALASLNGVD
                     STFQTPAVQSFLVVTEAFNAGGAPSAPFGHIGALLKATAPRLPGPNSGPEMGAAIQVL
                     KNAMTGTEAILPDSLLGKTFVWDTAQAKYVLSDSTGAPANGIRFELYAVGLDGHIAKP
                     LVQVGHLDLIDKSTASAQSLEVVVASATFTYIDYTVTGSGTLSSFTLTASGFIRNSTR
                     ELDFSISYAVTGSSCAITEQFADAADNAHLDFSFGITTTSDTSHTVTLNFDYQVGGHS
                     IALNGGGTVDSLTENLQATVKVDGNVFAIITASGLRETVVPTITDGNGQPLSDANRLL
                     LFKMFIMVGRSLEWLNNFVGPFVALAAIGVLLSL"
     gene            6766..8058
                     /locus_tag="VFU75_01265"
     CDS             6766..8058
                     /locus_tag="VFU75_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5465487.1"
                     /translation="MSSRRDFLRSIGVASATIPSLRNDALRHLQRVMPRVEGRSAAAI
                     AEDEDFWVTIQQAFDVDRSLVNLNNGGVAPSPRVVQDALRRYIEFSNNAPAMNMWGVL
                     EPEIEAVRSRLAATFGCDPEEMAITRNASEALENVQLGMPLNRGDEVLTTTQDYPRMI
                     TTWQQRVRREGIVLKQITFPVPPSSPDDLVARFAAGITPKTKVIHVCHITNLTGQIFP
                     IKKIMGLARPKGIEVVVDGAHAFAQFPFTRDELDCDYYGTSLHKWLTAPIGTGFLYVR
                     KEKIPNIWPLMAAPPEMNGNIRKFEEIGTHPAANHNAVSEAVTFYQQIGAERKAARLR
                     FLRDRWFQRLSPLPGVKILTSTDPAQSCGLASFTPGALDVGKVVGYLWDKHRIIVTPI
                     NHPEFHCIRVTPNVYTTLAEVDRFCDAMETVMKKGLPA"
     gene            8055..8270
                     /locus_tag="VFU75_01270"
     CDS             8055..8270
                     /locus_tag="VFU75_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465488.1"
                     /translation="MTRAVILALLLGAAACGGGSNATAKAPRDTLTERQRDSALARSS
                     IPGHQAVGAAMRAADTTSTQVRGDTTP"
     gene            8267..8752
                     /locus_tag="VFU75_01275"
     CDS             8267..8752
                     /locus_tag="VFU75_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465489.1"
                     /translation="MKSRGAPALIVGLLAVGCAPGSPCRTTDFKNIRRYSERYVGQWA
                     VARGDTLTLTQIGDRFHLASFALDTDTVMFNKECHLSGRMIFTVPAETLAVSWFGVPE
                     HATIFGWPDNLGPFAGASVSFWGRDSLHGTVLFDQATDIQVTQGTTAQFWAGKAGVRK
                     P"
     gene            8788..9273
                     /locus_tag="VFU75_01280"
     CDS             8788..9273
                     /locus_tag="VFU75_01280"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5465490.1"
                     /translation="MPLTAAQRAALIDRYERGPALLAAAWAKVPAEARQWRPGPGRWS
                     AHEVIVHCGDSESNAMLRIRFLLTEANTRIQAYDQAHWAEALDYHTLPVEPAMAAVIA
                     VRANTLPLLRRMTETDWQRAGVHPESGPYNAERWLQVYAEHLEKHSGQIERNLEAWRN
                     K"
     gene            9270..9668
                     /locus_tag="VFU75_01285"
     CDS             9270..9668
                     /locus_tag="VFU75_01285"
                     /inference="COORDINATES: protein motif:HMM:NF015953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEU5465491.1"
                     /translation="MRGFITRLLITALGLWLAANVVHGITVTGWASLVIAALVLGLVN
                     AIVRPLLVILTLPITFLTLGLFLLVVNGLSFAFAAAIVPGFHVATLGSAILGALVVSV
                     TGWFANSFIGSSGRFERVRRIEISGRRLDE"
     gene            9661..10029
                     /locus_tag="VFU75_01290"
     CDS             9661..10029
                     /locus_tag="VFU75_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HEU5465492.1"
                     /translation="MSRPLGVLGGMFGGLAVMAGAFGAHGLRGRLSPDLLSVFETGAR
                     YQMYHALALIAVALLLERRPSGPLRASGWCFAGGTVVFSGSLYLLALTGTGWWGAVTP
                     LGGVALIVGWGLLAYGLWRS"
     gene            10017..11177
                     /locus_tag="VFU75_01295"
     CDS             10017..11177
                     /locus_tag="VFU75_01295"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEU5465493.1"
                     /translation="MAELTVRGRRILLAAILLYAVVTIPLAVHKGEDIASEFAQSDLL
                     LRGLPVYLAPQNQGVWWPPFALAAIAPFALIARLSVPFAKGCWAAFSVLCLAWSLWRT
                     RRFGGRAMTFALLAVALPIQYNFQHLNINAPLLALAIAAADDFEGNERRAGVWVALAT
                     ALKLFPGLLILLFAFRKKWKAFGVAAVGTLLLTYASMLPYGVAGAAQAITRWVRLDLQ
                     ATSYQGAAVAALGMQKLSRLVYALDGNIVAVVVAHLALLALVWWALRRNAERGTRNAE
                     PGSAGSAFRLPSSAFADVGIVLVAACLLSPIGWLHTFTLAYPLWVFGIATLEPGRPWW
                     RTASWVIAGVLTSGYLAKIPWPHTIAWIAGHNDTIGSLLLLALVATVPRAQE"
     gene            11174..12307
                     /locus_tag="VFU75_01300"
     CDS             11174..12307
                     /locus_tag="VFU75_01300"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEU5465494.1"
                     /translation="MTLRPLTTRERNTLLGLALVYAAVVIPVGIHRGGDLVQEILQSD
                     RLLRGLPLYGQNPEKGVYWPPFALFALTPLALIARWSMAAAQAIWAVFNVWCVVWCLA
                     YAGRRWSWGVAVISLLAVAKPLQANFEHQNITVVLLYLVMLCHQALERGRAATAGGWV
                     GVATALKAFPGLLLVYFALRRRTKVFASGVLVAGVLTVGAMLRYGPVGAVDTVWSWFS
                     TSRTAPIMAGFGTQPLGNLIHVGLGLETPWVIGIEIVAGLILLWSILETPAGHDWIGD
                     LGLVSVFAALITPIGWFYYQTLAYPAWIGVLTVRPPARHWARTTVTAIAGVLLSGILT
                     SDHLYPEWMQLIKRFNYVWGALLLLAVLAFDRFTARLTPESKE"
     gene            12304..13386
                     /locus_tag="VFU75_01305"
     CDS             12304..13386
                     /locus_tag="VFU75_01305"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU5465495.1"
                     /translation="MIETPALLLHLDVVERNLATMAGRARNLGIALRPHIKTHKCVEL
                     GRRQVAHGARGVTVSTLDEAEVFADAGFDDILWAFPIDPGHIPHARRVAERTRLGVVV
                     DDLDTARALRNTQLTAWLKVDCGYHRAGVDPSSPYALEVVRALTGPGARFGGIVSHSG
                     HAYKTRGRPEAAGVAEEERLVMTAFADRLRSMGIEVPAVSVGSTPAMMALERGDGLTE
                     GRPGNYVFFDRTMVEIGVCESRDVGVTVLATVVSHQPGSKQFIVDAGALALSKDVGPT
                     HLGLSPAMGVIKDHPELAMVSLSQEHGIVHARTPAEIEGRFKVGETVEIVPNHSCLTV
                     AHFDHYHVMEGGKETTAWTISRNRGT"
     gene            complement(13407..14486)
                     /locus_tag="VFU75_01310"
     CDS             complement(13407..14486)
                     /locus_tag="VFU75_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HEU5465496.1"
                     /translation="MAAQRDYYEVLGVPESAGTDDVKKAFRRLAKQYHPDRNPNNPQA
                     AERFKEINEAHDVLSDAEKRKKYDTLRRYGAFAGGGARGPRPGGGPTPDEGMDFSNLG
                     SFGGLGDLFSSIFGGRPGGGREEGDETVETALHIPFRVAALGGKVPVTLPMAEVCPTC
                     GGKGGAPGSTIVTCSECKGRGTISFGQGGFAVNRPCPVCRGKGRIPSEPCPTCRGAGE
                     VRVEKRVTITVEPGTEDGTRVRLKGQGAKGKGDLIVNFHVEADPFFRRDGLDVICVVP
                     INVAQALLGTRIKVKTLDGKRVVLRVPPGTQPGQKFRIAGQGIAKGGRKGDQFVEVRL
                     DLPEQLTEEQAAAAKAFAEKAGLKY"
     gene            complement(14486..15037)
                     /locus_tag="VFU75_01315"
     CDS             complement(14486..15037)
                     /locus_tag="VFU75_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEU5465497.1"
                     /translation="MTEPTSSAENQNPPEEGGSVPPPVELPDAALQRVTEELAALKDK
                     YLRSAAEFDNFRKRTAKERVEVWAKAQGELIERLVDGLDDLNRFAEVDQATTDAKTLH
                     DGVELVRKKFWKVLDAVGLVRIDQTGVPFDPKVHEAVTMGAATAAEQDHTVGAVLQAG
                     YRMGETLIRPARVMVLSWKGEQS"
     gene            complement(15120..15950)
                     /locus_tag="VFU75_01320"
     CDS             complement(15120..15950)
                     /locus_tag="VFU75_01320"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5465498.1"
                     /translation="MPTFSAPDGLQLVFDAVEAPHPKAAVLVIHGWSDHAGRWTETAK
                     ALHDAGYSAYLLDLRGHGRSSGRRGHLSRFSQLLGDLHAFRRVVRVRTGAPQVLLGHS
                     FGGLVALRYLETQPLEPLAATVVASPFLGLGFRVNPLKLLAGRLLADLAPAVSLPTGI
                     DAEALCRDPAVCGAYRTDPLVHQRMTAGAWKEIQWAQRAVAADAHRIEGPIQFLLSGE
                     DRIVDAEMARAFADGLHGNVQVRWYPEMYHEALHDPQRERVLADVLTFLAGTAPKALS
                     "
     gene            complement(15950..17200)
                     /locus_tag="VFU75_01325"
     CDS             complement(15950..17200)
                     /locus_tag="VFU75_01325"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEU5465499.1"
                     /translation="MTIGTELLLGHTIDTNAAELGRALGAAGVEVVRRTTVPDRPKAI
                     ADAIHAALERTGFVLATGGLGPTKDDMTKKVVADLFGKPLYLDEGLLKALEARFKRLG
                     RGPMPAVNRNQAEVPEGATVLPNPRGSAPGLWIEDGGRVVILLPGVPSEMRGLLVEEV
                     LPRIIARQGKDGGARVVRSRTLRTTGVSESALAERVGPLEEALAPLSLAYLPSVEGVD
                     LRLTAWSVPPEEADRLLASGGDRLKKELGDHYYGEEETLLAATVLDILRTKGKTLAVA
                     ESCTGGMVGARLTAIPGASAVFTGGVVGYDDNVKREQLGVPSEVLEQHGAVSEEAARA
                     MAAGAARVFKTSCAIAVTGVAGPGGGTPEKPVGTVWMAILVDGTTTAVKRTFPGSREE
                     IRSRSAQTALDLLRRRFLPGGPGR"
     gene            complement(17212..17859)
                     /gene="pgsA"
                     /locus_tag="VFU75_01330"
     CDS             complement(17212..17859)
                     /gene="pgsA"
                     /locus_tag="VFU75_01330"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEU5465500.1"
                     /translation="MQWTWTLPNILTIARICLTPIIALLPFIEGYWPKVICFFIFIAA
                     AVSDVVDGYYARKHNQISELGQLLDPVADKLLLFAMLVPIFWLTRHPTITVDYRIPWW
                     GSMPVWVLLLLVGRELLITGFRWFAKRRGIIIPAMGPGKLKATVQNIFIGATIAWFAW
                     KDALAQFGWNGWWRNAWNELHGWFVTITLAIAVVLTVYSLIVYLYRYRQLLKDAL"
     gene            complement(17871..18539)
                     /locus_tag="VFU75_01335"
     CDS             complement(17871..18539)
                     /locus_tag="VFU75_01335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylation factor-like protein"
                     /protein_id="HEU5465501.1"
                     /translation="MINYASREINCKLVYYGPGLGGKTTNLEHVYGKVAPNTRGKMIS
                     LATETERTLFFDFLPVDLGTIRGFKTRFHLYTVPGQVYYNASRKLILKGVDGIVFVAD
                     SQIERMEANVESMQNLYDNTKEYGYDLTKIPFVIQYNKRDLPNASPIKDLQAALNPGW
                     IVEDAAQQKVVADPAHPGENLVEKVDSEWMGRAPYMEAVAVRGDGVFDTLKAVSKMVL
                     KTLS"
     gene            complement(18561..19046)
                     /locus_tag="VFU75_01340"
     CDS             complement(18561..19046)
                     /locus_tag="VFU75_01340"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEU5465502.1"
                     /translation="MTGAASWSFSENDFKQIQTHLQAFLRESNARCALLVDRTGQLVA
                     TVGEQPQFDPTAFASLTAADFSANDQLAKMIGEAEFASLVHQGEKESMFLADVARRVI
                     LVVLFDQRATLGLVKLKAKGAVENLNKVFEAMFNRVASGATPQKGILEGAEDEIDKLF
                     G"
     gene            complement(19063..19659)
                     /gene="recR"
                     /locus_tag="VFU75_01345"
     CDS             complement(19063..19659)
                     /gene="recR"
                     /locus_tag="VFU75_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HEU5465503.1"
                     /translation="MSAIEDLVTELAKLPGIGRKTAQRLTFHLLKQPADDAGRLADAI
                     RRVREQVRACSTCGTLSDQDPCGICADPRRDETILCVVEEAADANAIERAGRYRGRYH
                     VLGGRMSPLDGVGPEDLSGDSLLARVGNGHGVREVIVATNPSMEGDVTATWLQQLLAP
                     TGVRVTRIARGLPVGGDLEYADGVTIAQALSARREMTD"
     gene            complement(19666..19983)
                     /locus_tag="VFU75_01350"
     CDS             complement(19666..19983)
                     /locus_tag="VFU75_01350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEU5465504.1"
                     /translation="MADLSSLLQIAQQAQGRLSEIQTQLAQQTVTASAGGGIVQVTAD
                     GQGRIRAVRIDPEAVKQGDVEMLEDLVLAAVSEAQKRAAELAQAEVKKLAPGLPFPFQ
                     LPS"
     gene            complement(20021..21682)
                     /gene="dnaX"
                     /locus_tag="VFU75_01355"
     CDS             complement(20021..21682)
                     /gene="dnaX"
                     /locus_tag="VFU75_01355"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEU5465505.1"
                     /translation="MIALARKYRPTQFADLVAQDHVAAALRGAVASGRAAHGYLFTGP
                     RGVGKTTAARVLAMALNCPNRKDGEPCGTCESCSRIWTGAANLDVVELDAASNRGVDD
                     ARDLRERAMYAASADNRHKVYIVDEAHMLTREAWNALLKVLEEPPTGVVFVFATTDPQ
                     KIAQAAAPVLSRVQRFDFRRIGPHAIVDRLKHVLAQEKLKADDDALHLIAKSADGGMR
                     DALSLLDQVLSFGTGPVTAARVREVLGLIPDERYGELLRIIAEHDAKGAFALVDHLLE
                     AGASLEEFVAGAGEALRAVLLLSVGGEPEGLTEGMAGTLKAYAPRFSTGDVVRLLAML
                     AESEGQIRHSGNQRLVVELLVLRWALLDQTVQLAEVIRALGDPGASSPAPRRGPPAPP
                     PAAPTPAPAPSGRAPELRDSAPPRPSTSAERGAFTLERARQLWPGVVEQSRFTSPLLP
                     TLLADSEISAVHEGVVVLRIAAAHKDGIERKRESITRLLEPHFTGPVRIELESVAAPA
                     PVGNQGAGPARLTEATANAERLRVLRAKDPTLSAAVDALDLEILE"
     gene            complement(21679..23118)
                     /locus_tag="VFU75_01360"
     CDS             complement(21679..23118)
                     /locus_tag="VFU75_01360"
                     /inference="COORDINATES: protein motif:HMM:NF025017.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5465506.1"
                     /translation="MILGWIAHAIAWFGNFILVYFLALNLSYLILFLVSLVEVVRFLK
                     RTFFSDYQQILRSDLTLPISLLVPAHNEENTIVDTVRSLRMLNYPQFEIIVINDGSTD
                     HTLERLLDAYELRRVDRVFKRSLPTKPLRGVYASPLVPGLTVVDKERGGKPDALNIGI
                     NLSRYPLFCSMDADSVIEEDALLKAVKPFMEFPDETIAVGGIVRCVNGCTVKEGRVTR
                     IAIPRRALPIFQVVEYLRAFLGGRLGWSALQALPLISGAFGVYRKQAVIDVGGYDGTT
                     DTEDLELVLAMHQYRRARGLPYRIVFVPDPVCWTQVPERWRDLRRQRNRWHRGMLQSL
                     LRHRRMFLNPVYGIIGMVVLPYYLVFEALGPLIEGLGYLTVIVGAATGLLNGAFFVKF
                     FLVAVLVGVFLSVASVFLEEISFRRYPSWSDLLKLMLFGLLENVGYRQILAVFKLQAY
                     WEFVVGRRRWGRMDRRSFEPHALLDSPQR"
     gene            complement(23115..24341)
                     /locus_tag="VFU75_01365"
     CDS             complement(23115..24341)
                     /locus_tag="VFU75_01365"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU5465507.1"
                     /translation="MQEFVGVLLGALSGLFLLVFSVVLVTKAWRELRDGYERRRRALL
                     LPSIVAYANATEGPITDFVPTPLKRWERRVVEWLLLDHIRSLKGHSQARLALAFEQLG
                     FVALAHRQLSNRRWWLRVEGAEKLGRMLARPAVHDLVKLMDDPVPEVRLRAAKALGAI
                     GGLEAVETLIAALKATDRWATLRIADILAGLGPSAVEPLLEAFPRLPAAARVPAIDIL
                     GRLRSHLSVPLLRGLLADSDPNARARAANSLGLIGHPAVTQALIEALADPEWPVRAMA
                     AKALGRIAGDDSVEPLRRALSDPEWWVRANAAESLRGKGEAGRRALLVALDGDDAFAA
                     HKAAWMLQEAGVLDALLVRLGEGNEADRREALGVLRRLVALGRTDLLRDIAVRHPNPE
                     VRREVGSLLKTQAASA"
     gene            complement(24346..24771)
                     /locus_tag="VFU75_01370"
     CDS             complement(24346..24771)
                     /locus_tag="VFU75_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5465508.1"
                     /translation="MAPLLPEVNDTASRGGRILSIDDDAAIRQFLSAVLKADGYEVLL
                     AADGAAGVTTAKAELPDVILLDLIMPYASGFEVLRHLRDDEATRDIPVLVLSTQGGED
                     DIVRALDQGAEDFLIKPFYARELLARVRKVLTRARVARS"
     gene            complement(24780..24879)
                     /gene="ffs"
                     /locus_tag="VFU75_01375"
     ncRNA           complement(24780..24879)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VFU75_01375"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(24912..>25268)
                     /locus_tag="VFU75_01380"
     CDS             complement(24912..>25268)
                     /locus_tag="VFU75_01380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465509.1"
                     /translation="VIRAAIGQIFRSATLYPAAASPSRLEFHLGPNAIGDTLRLAPDT
                     TATVRVVATDEFGNAVALVGLQSLSGDEGIIRVERVGGDTVAGTLSVRARGQGSAQLA
                     VQGSGIFSNLTVIVGH"
BASE COUNT         4107 a         8732 c         8227 g         4202 t
ORIGIN      
        1 gtcgcgttcc tgatcgccgt cgtcgccatc atccagggga tgaacgccta cgtccgggac
       61 cgcatcggcg gcgccatcgt gggcgtcaac gcgtttcagg tccgccgcac gcccctcgcc
      121 ggtggcctga tcgatgacga agagtggaaa gcgattcagc ggcgccccat catcgctccc
      181 gaggacgtgc ccatcgtcga gcgcgccatc ccggacgcct tcgccgtggg cctgcagtcc
      241 ggatggccca ccccccaggc cgacgtggtg tggggagaca agacggtcgg ggacgtccag
      301 atgttcgggg tcaacgcctc ctaccaggtg gtgcaggact acaccttcgc ggcgggagac
      361 ccgctcaccg atgtcgacga ccgcgaacgc cggcccgtga tcgagctggg ctgggacatc
      421 tccgacaagc tctttggtga cccggacttc gcggtcggca agaagctccg gatcatgggc
      481 cagcagttca cggtgaaagg tgtggtcgcg aagaagggaa cggtcctggg catctcccaa
      541 gacggcttcg tgatgatgcc ctactcgacc ttcgagtccc tgtacggacg ccggaagacg
      601 acggtgatct ccgtgaaaat gacggacgcg gccgcggtac agccggccat gcagcgcgcc
      661 gaggaggcga tgcgcatcgc gcatcggctg cgcccgtcgc aggccgatga cttctcggtc
      721 agcacgtcgg acgcgttcgt gaccttctgg gagaatctca cccgggtcct gttcacggtg
      781 gtgcccgtcg tcgtcgcgat cgggatcgtc gtcggcggta tcgtgatcat gaacatcatg
      841 ctgatgagcg tcaacgagcg cacccgtgag atcggcatcc ggaagtcgat gggtgcgacg
      901 cggcgcgaca tcctgaacca gtttctggcg gaatccgtca cgctcgccct gctcggcggc
      961 ctgctcgggc tgctcgccgg cgggggcctg gccaccctgg tcgagctggt cacccccctg
     1021 cccgcgcggg tcaccctctg gtccgtattc ctggcgctcg gactcggcgc cggggtgggt
     1081 gtggtcttcg gggtgtaccc cgcgacccgc gcggcgcgcc tggatcccat caacgcgctc
     1141 cgggccgaat gagactccat tcggtctcgg agggctggca gatcgccgtc gatcagctcc
     1201 gcggcaacaa gttgcgctcc ggtctcacca tcctgggggt cgtgatcggc gtcgccacgg
     1261 tgatggggat ggcgtcgatc gtgcagggta tccgcgacca gatcatcaac accatcgagg
     1321 tcgccggccc tacgacgttc tacgtgatgc gctttttctc gcaaacgccg ctgaacccgg
     1381 atcaactccc caaggaggta cgcatccggc cggtgttgaa gccggccgag gccaaactgg
     1441 tcgcgggact cccaggaatc ggctacgccg gcatgtgggt gcagctgttc aacaagatgg
     1501 agttccaggg tgagcgcacg cagctcatga ccgagttcgg agccgacagc cactatatgg
     1561 agatccaggg aggcgagctg ctcgaagggc ggttcttcac gtcgtccgaa gaagcgagcg
     1621 gcgcgccggt ggtggtactc gaacgtgccc ccgccgacaa gctcttcggg cgcatcgatc
     1681 ccatcggacg gttcgtccgt gtcggcggga actccctcga ggtcgtgggg atttatcaga
     1741 atcccgcgaa catctttcag ccgccctccc agcagatcgg cgcgatcatg cccttcgaga
     1801 cggcgcgccg ctttttccgg tacgaccaga ccaacgcgtt gtggatcgtg gtgaagccgg
     1861 tcggcggcgt gaccgtcggt gaggcggaag acgaggtgac caccgcgttg cgccgctccc
     1921 gcggcctcgg gcccggcgcg cccaacagct tcgatctgct cacccaggat cagattctga
     1981 acatcttcaa cagcttcacc agtacgttct tcctggtgat ggtcgccctg tccagtgtgg
     2041 cgttgatggt cggtgggatc ggcgtcatgg cgatcatgat ggtctcggtc acggaccgga
     2101 cgcgggaaat cggggtccgg aaggccctcg gcgccacacg cgtcgagatt ctgtggcagt
     2161 tcctgatcga ggcgtcgacc ctcacgctcc tcgggggcct cgtcggcatc ctcatcggcc
     2221 tggtgttcgg acaggtcatc aagctggtcc tgcatttcga ctcgggtgtg ccggtctgga
     2281 gtgccttggt ggcctgtctg gtgtcggtgc tgatcggcct gatcttcggt gtgctcccag
     2341 ctcaacgcgc ggccaggctg gatccggtcg aagcgctgag atacgagtaa aggcgtggga
     2401 ggtgggacgt gggacgatgg taccacaccc ccacctcccc acgcctttcg gtagattcca
     2461 tcccatgacc gacacgatcg aaaccgtcga tctcctcgcc atcgccgccc accgcgacga
     2521 cgtcgagctg acctgtgggg ggacgctggc caggaccgcg cagaaggcac gcgtgggcat
     2581 cgtggacctg acgcaaggag agatgggcac acgcggcgac gcggcgatac gggcggccga
     2641 agcggagcgg gcggcgcggg tgctgggtgt ccacgtacgg ttgaacgccg gcctccccga
     2701 cgcacacatc tacaacgacg atccctcacg aaagaccgtg atcgagctga tccggaggac
     2761 caggccgcgg gtggtgattc tgcccttccc gatcggacgg catccggatc accgcatcgc
     2821 ctcggagctg gcacgcgacg cctgctacct cgccggcctc aagaagtatc cggcgtcagg
     2881 agagccgcac cggccccaca agattctgta cgcgctcgcc taccgcgaag acacggtgaa
     2941 gccgtccttc gtggtcgaca tctcggatgt cttcgaaacg aagatgaagg ccatccgctg
     3001 ttacgcgtcg caattcgacg gggctcaggc cgccggagag atctacccca ccgggcagca
     3061 cctctacgac ctgattcagg tgcagtccgc ccactacgga tcgctcatcc ggcgccccta
     3121 cggcgagccg tacttcacgc aggagaccgt ggcggtggac gacgtcctct cacttggcgt
     3181 ccagtccctc tagtcgatag attttcccca tgaccgctgc caagcgctcg gtttacctcg
     3241 accacgcggc gaccactccg gtgcgccccg aggtcctgga agccatgctc ccctacctcg
     3301 gcgtcgaggc cttcgggaat ccgtcctccg cccatcgcgt gggccgggcg gcgcgggcgg
     3361 gcgtggaaga agccaagcgg cgcgtcgcgg aagcgctcca cgtcgaaccc aaccaggtca
     3421 ttttcacctc cggtggcacc gaggcggaca acctcgccat catcggctcg gccctcgcgg
     3481 ccaaggatca tggcggccat ttccgcgtcg ccgtctcggc caccgaacac aaggcggtcc
     3541 tcgccgccgc ccacgcggtg aagcacctcg ggggggacga gatcatcctg ccggtggact
     3601 cggacggcct cgtggacgag cgcgcggtcg aggacgctct ccggcgcggg gtggccatcg
     3661 tcagcgtcat gtgggtgaac aacgaggtcg ggaccgtcca gccgatcgcg aagctcgcgg
     3721 cccgatgtcg cgaggcaggc gtcgcgttcc actcggacgc cgttcaggcg ttcggcaaag
     3781 ttcccgtcga cctcgcggaa gttcccgtca ctttcctgac catctccggc cacaagatcg
     3841 gcgcccccaa gggcatcggc gcgctcatcg tgcgcgaccg ccacgctctg gaagcgatca
     3901 tccatggcgg cggccagcag ttcggcatcc ggcccggcac cgagaatgtc cccggcatcg
     3961 tggcgctcgg acgggcggcg gagctggcgg ccaaggaaca ggccacgacg gcggcccgcg
     4021 tggcgaagct gcgcgatgac ctcgaagcgc gcttcctggc cgtggtggag ggcgcggtgg
     4081 tgaacggcag gaacgccccc cgcgcgccgc agaccgtgtc gttctcaatc ccgggcgccg
     4141 acggcgaagc actcctgatg cacctggatc tcgcgggaat cgcctgctcc ggtggctccg
     4201 cgtgcagcac cggatcggtc gacccctccc acgtgctcac cgcgatgggg atcgcccggg
     4261 acatgggcag tgccgcactc cgcttctccc tcggacgcga gtccaccacc gaagacgtcg
     4321 acgcgctcat cgaagccatg ccgaaggtgg tggcgaaggt tcgcgccctg accggagccc
     4381 tgggacgtgc ctgaacgggt tctggtcgcg atgtcggggg gtgtcgactc ctcggtcgcg
     4441 gccgcgcttc tggtcgagca gggctacgaa gtcatcggcg ccacgctgaa gctgttctgc
     4501 tacggcgacg aggtcgccga ccgtccctgc tgctcgctcg attccatcac cgacgccaag
     4561 ctcgtcgcca accgcctggg catcccgcac tacgtcttga acctcgaaga ccggttcggg
     4621 cgcgacgtga tcgggaactt cgtgtccgag tacgcggcgg gccgcacgcc catcccctgc
     4681 gtccggtgca actcgttcac caagttccgc gacctgctcc tccacgccga cgcgctcgac
     4741 tgccgttacc tggccaccgg gcattatgcg gtggcgcggt ccggggagct gcaccggggc
     4801 cgcgaccgca gcaaggacca gacctatttc ctctggggca tcgaccggtc agtggtcgcc
     4861 cgcatgctga cgcccgtcgg cgcgctcacc aaggcggaga ccagggcccg cgcccgtgag
     4921 ctcggtctcg tcaccgccga aaaacccgag tccgtcgaga tctgtttcgt acccgacgat
     4981 gactacgtga aggtgctgga gcgccgcctc cccgccgacg cgccggcgct ctcccccggc
     5041 gacttcgtga cgcgcgatgg caccgtactc ggcgctcacg ccggattcgc ccggttcacc
     5101 gtcggacagc ggcgcggact tcccggcggc ttcccggtgc ccatgttcgt gacggcgatc
     5161 aggcccgaaa cccgcgaggt cgtcatcggg cccgccgacg agctgctcgg gcaccaggtg
     5221 attctcgaag aggtcaactg gatcgcggaa cccctcgcgg ccgacgcccg ggtgtgggtg
     5281 cagtgccgct atcgcgcggc cgcggtgccg gccgtcgtcc tcacgaccca gggtgaccac
     5341 ctgaccctgg ccctcgagga accggttcgc gccatcacgc ccggtcaatc cggcgcgctg
     5401 tacacccggg aaggccgcct tctgggggga ggagtgatcg ccctggccgc caccaggtgc
     5461 gccacgtcac cgagccgccg cctcgccgtc ctgaatagtt aggtcctccg gtttcctgcc
     5521 tcacccaagc tcgaggattg aacatgtccc ccatccgtaa acgaatcgcc ggtgccgccg
     5581 tcctgctgct ggcggccgcc gcgtgcagca aagacgccac cgccccgaag ccgctcgatc
     5641 ccacggccgc gctggcatcg ctcaacgggg tcgacagcac cttccagacg ccggccgtcc
     5701 agagcttcct cgtcgtcacc gaagccttca atgccggtgg cgccccgtcg gcgccgttcg
     5761 ggcacatcgg cgcgttgctc aaggccaccg ctccccgcct cccgggtccg aattcgggtc
     5821 cggaaatggg cgcggcgatc caggtgttga agaacgcgat gacggggacc gaggcgatcc
     5881 tgcccgactc cctgctcggc aagaccttcg tctgggacac cgcgcaggcc aagtacgtct
     5941 tgtccgacag cacgggcgcg ccggcgaacg gcattcgctt cgagctgtat gccgtggggc
     6001 tcgacggaca catcgcgaag cccttggtgc aggtcgggca cctcgacctg atcgacaaga
     6061 gcaccgcgag cgcacagagt ctcgaagtcg tggtcgcgag cgccaccttc acttacatcg
     6121 actacacggt gacggggtcc ggcacgctct cgagcttcac gctcacggcc tcggggttca
     6181 tccgcaactc cacccgtgag ctggacttct cgatctcgta cgccgtcacc ggatcgagct
     6241 gcgcgatcac ggagcagttc gccgacgccg ccgacaacgc ccacctggac ttctccttcg
     6301 gaatcacgac gacgtccgac acgtcgcaca cggtcaccct gaacttcgac tatcaggtgg
     6361 ggggacactc catcgccctg aatggcggcg gcacggtcga ttccctcact gaaaacctgc
     6421 aggcgaccgt gaaagtcgac ggcaatgtgt tcgcgatcat taccgccagc gggctccgcg
     6481 aaacggtggt tcccaccatc accgacggga acggccaacc cctgtcggac gccaatcgcc
     6541 tcctcctgtt caagatgttc atcatggtgg ggcggtccct ggagtggctc aacaacttcg
     6601 tggggccgtt cgtggccctg gcggccatcg gggtcctcct gagcctctga caactagacg
     6661 tctagttcgc gcaccaccgt tcccccatga aaagtcccgc ccggctctag ccggacggga
     6721 tttttcgttg tattcctgtc tggtcaatca ccccgagatc cgaccgtgtc gtcacgtcgc
     6781 gacttcctgc gctccatcgg agtagcctcc gccaccattc cctcgctccg caacgacgcc
     6841 ctgcgccacc tccagcgggt gatgccgcgc gtcgagggcc gttccgcggc cgcgatcgcg
     6901 gaggacgaag acttctgggt gacgattcag caggcgttcg acgtcgaccg ctcgctggtc
     6961 aacctcaata acggcggcgt ggcacccagt cccagggtgg tccaggacgc cttgcgccgg
     7021 tacatcgaat tctccaacaa cgcgccggcc atgaacatgt ggggcgtgct ggaaccggag
     7081 atcgaggcgg tgcggagccg cctggccgcg accttcgggt gcgacccgga agagatggcc
     7141 atcacgcgga acgccagcga agcgctcgag aacgttcagt taggcatgcc cctgaatcgg
     7201 ggcgacgaag tcctgaccac gacgcaggac tacccgcgga tgatcacgac ctggcagcag
     7261 cgggtccgcc gcgaagggat cgttctgaag cagatcacgt tccccgtacc gccctcatcg
     7321 ccggacgatc tggtggcgcg ctttgccgcc gggatcacgc cgaagaccaa ggtcatccac
     7381 gtctgccaca tcacgaacct gaccggacag atcttcccca tcaagaagat catgggcctc
     7441 gcgcgtccga aggggatcga agtcgtcgtc gacggcgccc atgccttcgc ccagttcccg
     7501 ttcacgcgtg acgagctcga ctgcgactac tacggcacca gcctgcacaa atggctgacg
     7561 gccccgatcg ggaccggctt cctgtacgtc cggaaggaaa agattccgaa catctggccc
     7621 ctcatggccg cgccacccga gatgaacggc aacatccgga agttcgagga aatcggcacc
     7681 caccccgccg cgaaccacaa cgcggtctcc gaggccgtga cgttctacca gcagatcggc
     7741 gccgagcgga aggcggcgcg gctcaggttc ctgcgggacc ggtggttcca gcggctctcg
     7801 cccctgcccg gcgtgaaaat cctgacgtcc accgatccgg cccagtcgtg cggcctggcg
     7861 tcgttcacgc cgggggcgct ggatgtcggc aaggtggtcg gctacctctg ggacaagcac
     7921 cggatcatcg tgacgcccat caatcatccg gagttccatt gcatccgtgt cacgccgaac
     7981 gtctatacga cgctcgccga ggtcgaccgg ttctgcgatg cgatggaaac cgtgatgaag
     8041 aagggactcc ccgcatgacc cgcgccgtca tcctggccct cctgctcggc gccgccgcgt
     8101 gcggcggggg ctcgaacgcg accgccaagg cgccgcggga cacgctgacc gagcgccagc
     8161 gggacagtgc cctggcccga tccagcatcc ccggtcacca ggccgtggga gcagccatgc
     8221 gggccgcgga caccaccagc actcaggtcc gaggcgacac gacgccgtga agtcccgcgg
     8281 cgcaccggcc ctgatcgtcg gtctgctggc ggtcgggtgc gcgccggggt ccccctgccg
     8341 caccacggat ttcaagaaca tccggcgcta ttcggagcgg tacgtcgggc agtgggcggt
     8401 cgcgcgtggc gacaccctca cgctcacgca gataggcgac cggtttcacc tggcgtcgtt
     8461 cgcgctcgac accgacaccg tcatgttcaa caaggaatgc cacctttccg gccgcatgat
     8521 cttcaccgtg cccgcggaga cgctggcggt gagctggttc ggcgtgccgg agcatgccac
     8581 gatcttcggc tggcccgata acctgggacc cttcgccggc gcgtccgtca gcttctgggg
     8641 ccgggattcc cttcatggca ccgtcctgtt cgaccaggcc acggacattc aggtgactca
     8701 ggggacgacc gcgcagttct gggccgggaa agctggcgtc cggaagccct gattaccttt
     8761 cgggcgtccc cctccccggg agtgcccgtg ccgctgaccg ctgcccaacg cgccgccctg
     8821 atcgatcgct acgaacgtgg tcccgccctc ctcgccgccg cgtgggccaa agtgcccgcc
     8881 gaggcgcgcc aatggcgtcc gggtcccggc cgttggtccg cgcacgaagt gatcgtgcac
     8941 tgcggggatt ccgaatccaa tgccatgctc cggatccgct ttctgctcac ggaagcgaac
     9001 accagaatcc aggcgtacga ccaggcgcat tgggcggagg cgctcgacta ccacacgctg
     9061 ccggtcgaac cggcgatggc ggccgtcatc gcggtccggg cgaatacgtt gcccctgctc
     9121 cggcgcatga cggagaccga ctggcagcgg gcgggcgtcc accccgaatc gggcccctac
     9181 aacgccgagc ggtggctgca ggtgtacgcc gaacacctgg aaaaacattc gggccagatc
     9241 gagcgcaacc tcgaagcctg gaggaacaag tgaggggatt catcacccgg ctcctgatca
     9301 cggcgctggg tttgtggctc gccgcgaacg tcgtgcacgg gatcaccgtg accggctggg
     9361 cgagcctggt gatcgcggcg ctcgtgctgg gactggtgaa cgccatcgtt cgacccctgc
     9421 tcgtcatcct caccctcccc atcaccttcc tcacgctcgg cctgttcctc ctcgtcgtga
     9481 acgggctcag cttcgccttt gccgccgcga tcgtaccggg cttccacgtc gcgacgctgg
     9541 gaagcgcgat cctgggcgcc ctggtggtca gcgtgacggg gtggttcgcg aactccttca
     9601 tcgggtcgag cgggcgcttc gagcgcgtcc gccggatcga gatctcggga cgccgcctgg
     9661 atgagtagac ccctgggggt cttgggcggc atgttcggcg gattggcggt catggctggg
     9721 gcgttcggcg cccacggcct ccgagggcgg ctgtctcccg acctgctctc cgtattcgaa
     9781 acgggcgccc gctaccagat gtatcatgcc ctcgccctga ttgccgtcgc tctcctgctc
     9841 gaacgccggc cctctggacc gctccgcgcg tcgggctggt gcttcgcggg gggcactgtg
     9901 gtgttctccg ggagcctcta tctcctggcg ctcacgggga cggggtggtg gggagcagtg
     9961 acgcccctcg ggggcgtggc gctcatcgtc ggctggggac ttctcgccta cgggctatgg
    10021 cggagctgac cgtccgcggc cggcggatcc tgcttgccgc catcctgctg tacgccgtcg
    10081 tcaccattcc gctcgccgtt cacaagggcg aggatatcgc gagcgagttc gcgcagagcg
    10141 acctcctgct gcgcggcctt cccgtgtacc tggcgccgca gaaccagggc gtctggtggc
    10201 ctcccttcgc gctcgccgcc atcgcgccct tcgccctgat cgcgcgtctg tcggtcccct
    10261 tcgcgaaggg gtgctgggcg gcgttctcgg tcctctgcct cgcctggagc ctgtggcgca
    10321 cgcgccgctt cggcgggcgg gccatgacgt tcgcgttgct cgccgtggcc ctgccgatcc
    10381 agtacaactt ccagcacctc aacatcaacg cgcccctgct cgcactcgcg atcgccgcgg
    10441 cagacgactt cgaggggaac gaacgtcgcg ccggggtctg ggtggcgctc gcgacggccc
    10501 tcaagctgtt tccgggcctg ctgatcctgc tgttcgcatt ccgaaagaag tggaaggcct
    10561 ttggggtcgc ggcggttggc acactgctcc tgacctacgc ctcgatgttg ccatatgggg
    10621 tcgccggcgc ggcacaggcg atcacgcgtt gggtgcggct cgatctccag gcgacgtcct
    10681 accagggagc ggcggtcgct gcgctcggca tgcagaagct ctcgcgcctg gtctacgcgc
    10741 tcgacgggaa catcgtcgcc gtcgtggtcg cgcatctggc gctcctggcc ttggtgtggt
    10801 gggcgcttcg gcgaaacgcg gagcgtggaa cgcggaacgc ggaaccggga tcagcaggtt
    10861 ccgcattccg acttcccagt tccgcgttcg ccgacgtcgg catcgtcctc gtcgcggcct
    10921 gcctgctctc ccccatcggc tggctgcaca ccttcacgct ggcctaccca ttgtgggtgt
    10981 tcgggatcgc cacccttgaa ccgggccgcc cctggtggcg cacggcgtcc tgggtgatcg
    11041 ccggggtcct cacgtccggg tacctcgcga aaatcccgtg gccgcacacc atcgcctgga
    11101 tcgccggtca caacgacacg atcggctcac tgctcctgct ggccctcgtc gccaccgtcc
    11161 cccgggctca ggaatgaccc tcaggcccct gaccacccgc gagcgcaaca cactgctcgg
    11221 tctcgccctc gtctacgcgg ccgtggtcat tcccgtcggc atccatcgcg gcggcgatct
    11281 ggtccaggaa atccttcaaa gtgaccgtct cctcagaggc cttccccttt atggccagaa
    11341 ccctgaaaaa ggcgtctact ggcccccgtt cgccctcttc gccctgacgc ctctcgccct
    11401 catcgcccgg tggtcgatgg cggcggccca ggcgatctgg gcggtcttca acgtgtggtg
    11461 cgtggtgtgg tgcctcgcat acgcgggacg gcggtggagc tggggagtcg cggtgatatc
    11521 gctgctcgcc gtcgccaagc cgctgcaggc gaacttcgag caccagaaca tcacagtggt
    11581 cctgctctat ctggtgatgc tctgccatca ggcgctggag cgggggcgcg cggcgaccgc
    11641 aggcgggtgg gtcggcgtgg ccaccgcgct caaggccttc cctggactat tgctcgtcta
    11701 cttcgccctg cgccggcgca cgaaggtgtt cgcctccgga gtgctcgtcg ccggcgtcct
    11761 caccgtcggc gccatgttgc gctacggccc cgtcggcgcg gtggacaccg tgtggagctg
    11821 gttttccacc agccgaaccg cgccgatcat ggcgggattc ggtactcagc ccctcggcaa
    11881 tctcatccac gtcggactgg ggctcgagac gccctgggtc atcggcatcg agatcgtggc
    11941 gggattgatt ctcctctggt ccatcctcga gaccccggcg ggccacgact ggatcgggga
    12001 cctgggcctg gtcagcgtct ttgccgcgct catcactccc atcggctggt tctactatca
    12061 aactctggct tacccggcct ggatcggtgt cctgaccgtc aggccccccg cgcgccactg
    12121 ggcccgcacg accgtcaccg ccatcgccgg cgtcctgctg tcgggcatac tcacgtccga
    12181 ccatctgtac cctgagtgga tgcagctcat caagcgcttc aactacgtgt ggggcgcctt
    12241 gctcctgctc gcggtgctcg cgttcgaccg cttcaccgcc cgtctcaccc cggagtccaa
    12301 ggaatgatcg aaaccccggc tctgctgctc cacctcgacg tggtcgagcg caatctggcc
    12361 accatggcgg gacgcgcccg caatctcggc atcgccctgc ggccgcacat caagacccac
    12421 aaatgcgtcg agctgggacg gagacaggtc gcgcatggcg cgcgcggcgt gacggtgtcg
    12481 acgctcgatg aagccgaggt ctttgcggac gccgggttcg acgacattct gtgggcgttc
    12541 ccgatcgacc ccggccacat cccgcatgcc cggcgtgtgg cggagcgcac caggttgggc
    12601 gtggtggtgg acgatctcga caccgcccgg gccctgcgca atacccagct cacggcctgg
    12661 ctcaaggtcg actgcggata tcaccgggcc ggggtggacc cttcctcacc gtatgcgctg
    12721 gaggtcgtgc gggccctgac gggacccggc gcccggttcg gcggcatcgt cagtcactcg
    12781 gggcacgcgt acaagacgcg cgggcgaccg gaggccgcag gcgtggcaga ggaggagcgc
    12841 ctggtgatga ccgccttcgc ggaccggctc cggagcatgg gcatcgaagt cccggccgtg
    12901 agcgtcgggt ctacgccggc gatgatggcc ctcgagcggg gggacgggct gaccgagggg
    12961 cgccccggca attacgtgtt tttcgaccgg accatggtcg agatcggcgt ctgtgaatcc
    13021 cgcgacgtgg gcgtcacggt cctggcaacc gtagtgtccc accagcctgg cagcaagcaa
    13081 ttcatcgtcg acgcgggggc gctcgccctg tccaaagacg tcggccccac acacctcggc
    13141 ctctcacccg ccatgggagt gatcaaggac caccccgagc ttgcgatggt ctcactgtcc
    13201 caggagcatg ggatcgtgca cgccaggacg ccggctgaga tcgaaggacg cttcaaggtg
    13261 ggagaaacgg tggagatcgt gcccaaccac tcgtgtctca cggtagccca cttcgatcat
    13321 taccacgtga tggagggagg taaggaaact acggcatgga ctatctcaag aaatcgtggc
    13381 acctagccgt ctagccgtct agccgtctag tacttcagcc ccgccttctc agcgaacgct
    13441 ttcgccgccg ccgcttgctc ctcggtgagc tgctcgggaa ggtcgagacg cacttcgacg
    13501 aactgatcac ccttccttcc acccttcgcg atgccctggc ccgcgatccg gaacttctgt
    13561 cccggctgcg ttcccggcgg cacgcgcagt accacccgct tgccatccag cgtctttacc
    13621 ttgatccgcg tccccaggag cgcctgggcc acgttgatgg gtacgacgca gatcacgtcg
    13681 agcccgtcac gccggaagaa tgggtcggcc tcgacgtgga agttgacgat cagatcgccc
    13741 ttccccttcg cgccctgtcc cttgagacgc acgcgcgtac catcttcggt accgggctcg
    13801 acggtgatgg tgacgcgctt ctccacacgc acctcgcccg cgccgcggca ggtcgggcat
    13861 ggctcggagg gaatccgtcc cttgccacgg cacacggggc acgggcgatt caccgcgaaa
    13921 cccccctggc cgaacgaaat cgtgccccgc cctttgcact ccgagcaggt cacgatggtg
    13981 gatccgggag ctcccccctt gccaccgcac gtcgggcaga cctccgccat cggcaaagtg
    14041 accgggacct tgccccccaa tgcggcgacc cggaacggga tatggagcgc cgtctcgacc
    14101 gtttcgtcgc cctcttcgcg gccgccgcca gggcgcccgc cgaaaatcga cgagaagagg
    14161 tcccccagcc caccgaagga accgaggttg gagaagtcca tgccttcgtc gggagtgggg
    14221 ccgccgccgg ggcgaggccc ccgtgcacca ccacccgcga acgctccgta gcgccgcagg
    14281 gtgtcgtatt tcttccgctt ctccgcatcg gagagcacgt cgtgcgcttc attgatttcc
    14341 ttgaagcgct cggccgcctg cggattgttg ggattgcggt ccgggtgata ctgcttggcc
    14401 agccgccgga acgccttctt cacgtcgtcg gtgccggcgc tttccgggac tcccaggact
    14461 tcgtaatagt cgcgctgggc ggccatcagg actgctcacc cttccacgac agcaccatca
    14521 cccgggcggg acgaatcagg gtctccccca tccggtaccc ggcctggagc accgccccca
    14581 cggtgtggtc ctgctcggcg gccgtcgcgg cgcccatcgt gacggcttca tggactttgg
    14641 gatcgaatgg cacaccggtc tgatcgatcc ggacgagtcc gaccgcgtcg agcaccttcc
    14701 agaacttctt tctcaccagt tccacgccgt catgcagcgt cttcgcatcc gtcgtggcct
    14761 gatcgacctc cgcgaagcgg ttcagatcgt cgagcccgtc caccaggcgt tcgatcagct
    14821 ccccctgagc tttggcccac acctcgaccc gctccttggc cgtccgcttc cggaaattgt
    14881 cgaactcggc ggccgaacgg aggtacttgt ccttcaacgc cgcgagctcc tccgtcaccc
    14941 gttgcagggc ggcgtcgggg agctcgacgg gcggaggcac actgcccccc tcttcgggag
    15001 gattctggtt ctcagcgctg ctcgtgggtt cggtcatcag gcctcggagg agcgacgggg
    15061 gcaaacggca tgccctaaac ccccgccgaa atggcatgtc tggtggccgc aaaatagcat
    15121 tacgacaagg ccttcggagc ggttccagcc agaaaggtca ggacatcggc caggacccgt
    15181 tcccgttgag gatcatgcag agcctcgtga tacatctcgg gataccatcg cacctgcacg
    15241 ttcccgtgca gcccatccgc gaacgcgcgc gccatctccg cgtcgacgat ccgatcctcg
    15301 ccgctcaaga gaaactggat gggtccttcg atccggtggg cgtcggccgc cacggcgcgc
    15361 tgggcccatt ggatttcctt ccacgccccc gcggtcatcc gctgatgcac cagggggtcc
    15421 gtccggtacg cgccgcacac ggcgggatcg cggcagagtg cctcggcgtc gatccccgtc
    15481 ggcaaggaga ccgccggcgc gagatcggcg agcagacgcc cggcaaggag cttgagcggg
    15541 ttcacccgga agcccaggcc aaggaatggc gatgcgacga ccgtcgccgc cagcggctcg
    15601 aggggctggg tctcgaggta gcggagggcg acgagaccac cgaaggagtg ccccaggagc
    15661 acctgtggcg cgcccgttcg aacgcgcacg acacgccgga acgcgtgcag gtcaccgagg
    15721 agctggctga agcgcgagag atgtccgcga cggccggagg agcggccatg cccgcggaga
    15781 tccagcaggt atgccgagta gcccgcgtcg tgaagcgctt tggccgtctc ggtccagcgg
    15841 ccggcgtggt ccgaccagcc gtggatgacg aggacggcgg ctttcggatg cggcgcttcc
    15901 acggcatcga agaccagctg gagtccgtcg ggggccgaga aggtcggcat cagcgcccgg
    15961 ggccaccggg gagaaagcgt cgccggagga ggtcgagcgc ggtctgggca ctgcggctgc
    16021 ggatctcttc gcggctccct gggaacgtcc gcttcacggc ggtggtggtg ccgtcgacga
    16081 ggatcgccat ccagacggtg cctacgggct tctccggtgt cccacccccc ggccccgcga
    16141 cgcccgtcac cgcgatggcg cacgacgtct tgaagacgcg cgcggcaccg gcggccatcg
    16201 cccgcgccgc ctcttcggac acggcgccat gctgctcgag tacttcggag gggacgccca
    16261 gctgttcgcg cttcacgttg tcgtcgtaac cgaccacgcc cccggtgaac acggccgagg
    16321 cgccgggaat ggcggtcagc cgtgcgccga ccatgccgcc cgtgcaggac tcggcgaccg
    16381 cgagtgtttt tcctttggtc ctgagaatgt ccaggaccgt ggcggcgagc agggtctctt
    16441 cttccccgta gtagtgatca ccgagttcct tcttcaggcg atcacccccg gaggccagaa
    16501 ggcggtccgc ctcttccggc gggaccgacc aggccgtgag ccgaaggtcc acgccctcga
    16561 cggacgggag gtacgcgagc gaaaggggcg cgagggcttc ctcaaggggg cccacgcgct
    16621 cggccaacgc cgactcggag acgcccgtgg tgcgcagcgt acgggagcgc acgacccgtg
    16681 ctcccccatc cttgccctgg cgggcgatga tccggggcag cacctcctcg accagcagtc
    16741 cccgcatctc tgacgggacc ccggggagca gaatcaccac tcgcccgccg tcttcgatcc
    16801 agaggccggg cgcgctcccc cgcggattcg gcagcacggt ggcgccctcc ggcacctccg
    16861 cctggttgcg gttgacggcc ggcatgggac cgcgaccgag ccgcttgaag cgggcctcga
    16921 gggccttgag cagcccttca tccaggtaga ggggtttgcc gaacagatcg gccacgacct
    16981 tcttggtcat gtcgtccttg gtcgggccca gccccccggt cgcgagcacg aacccggttc
    17041 gctccagggc cgcgtgaatg gcgtcggcga tggctttcgg gcggtccggc acggtcgtgc
    17101 ggcggaccac ctcgacgccc gcggccccaa gggcccggcc gagctcggcg gcgttcgtgt
    17161 cgatggtgtg accgaggagg agctcggtcc cgatggtcac caactcgagg ttcagagggc
    17221 gtccttcagg agctgacggt accggtagag gtacacgatg agcgaataga cggtgaggac
    17281 gaccgcgatc gcgagggtga tggtcacgaa ccagccatgg agctcgttcc aggcgttccg
    17341 ccaccagccg ttccacccga attgcgcgag cgcgtctttc caggcgaacc acgcgatggt
    17401 cgcgccgatg aaaatgttct ggacggtggc cttgagcttt ccaggcccca tggcggggat
    17461 gatgatcccc cggcgtttgg cgaaccaccg gaagccggta atcagcagct cacgtcccac
    17521 gaggagcagc aggacccaga cgggcatgct gccccaccag ggaatccggt agtcgacggt
    17581 aatggtagga tgccgggtga gccagaagat cggcaccagc atcgcgaaga ggagcagttt
    17641 gtcggccacg gggtcgagga gctggcccag ctcggagatc tggttgtgct tgcgggcgta
    17701 gtagccgtcg acgacgtcgg agacggcggc cgcgatgaag atgaaaaagc agatgacttt
    17761 gggccagtac ccttcgatga acggcaggag ggcgatgatg ggcgtcagac agatccgcgc
    17821 gatggtgagg atattcggca gagtccacgt ccactgcatg cccgaactcc tcaactcaac
    17881 gtcttgagga ccatcttgct cacggccttg agggtatcga acacgccgtc gccgcgcacc
    17941 gccaccgctt ccatgtaggg cgcccgtccc atccactccg agtcgacctt ctcgaccagg
    18001 ttctcgccgg ggtgcgcggg gtcggccacc accttctgct gagccgcgtc ctcgacgatc
    18061 catcccgggt tcaatgccgc ctggaggtcc ttgatgggcg aggcattggg gagatcgcgc
    18121 ttgttgtact ggatcacgaa cggaatcttg gtcaggtcgt acccgtattc cttcgtgttg
    18181 tcgtacaggt tctgcatcga ctccacgttg gcttccatcc gctcgatctg gctgtccgcc
    18241 acgaacacga tcccgtcgac gcccttgagg atgagcttcc ggctcgcgtt gtagtagacc
    18301 tgccccggta ccgtgtagag gtggaagcgg gtcttgaatc cacggatggt accgaggtcg
    18361 acgggcagga agtcgaagaa gagcgtccgc tcggtctcgg tggcgagcga gatcatcttg
    18421 ccgcgcgtgt tgggcgcgac tttcccgtat acgtgctcca ggttcgtcgt cttgccgccg
    18481 agcccgggac cgtagtacac gagtttgcag ttgatctcgc gggaggcgta attgatcatc
    18541 gacatgtgat cgcgccccgc tcagccgaac agtttgtcaa tttcgtcttc cgccccttcg
    18601 aggataccct tctgcggggt ggcaccggac gccacccgat tgaacatcgc ttcgaacacc
    18661 ttgttcagat tctcgaccgc gcccttggcc ttcagcttga ccaggcccag ggtggcgcgc
    18721 tggtcgaaga gcaccaccag aatcacccgg cgggctacgt cggccagaaa catcgattcc
    18781 ttttcgccct ggtgcaccag cgacgcgaac tcggcttcac cgatcatctt ggcgagctgg
    18841 tcgttggcgc tgaaatcggc ggccgtcagc gaggcgaacg cggtgggatc gaactggggc
    18901 tgttccccca cggtggccac gagctgaccg gtgcggtcca cgagcagcgc gcaccgcgcg
    18961 ttggattcgc gcagaaacgc ctgcaagtga gtctggatct gtttgaagtc gttctcggag
    19021 aacgaccagc tcgccgcacc cgtcatcaca cccctcgggt ccctagtctg tcatctcccg
    19081 ccgagccgac agcgcctgcg caatcgtgac gccgtccgcg tattcgaggt caccacccac
    19141 cggcaggccg cgcgcgatcc gggtcacccg cacccccgtg ggcgccagca gctgctggag
    19201 ccacgtcgcg gtcacatccc cttccatcga ggggttcgtg gccacgatca cctcgcgcac
    19261 cccgtgcccg ttcccgaccc gggccaggag cgagtccccg ctcagatcct cagggcccac
    19321 cccgtcgagc ggcgacatcc gcccacccaa cacgtgatac cggccgcggt accggcccgc
    19381 ccgctcgatc gcattcgcgt cggccgcctc ttccaccacg cagaggatgg tctcgtcgcg
    19441 acgcggatcg gcgcagatgc cgcacggatc ctggtccgag agcgtgccac aggtactgca
    19501 ggcccgcacc tgctcccgca cccgccggat cgcgtcggcc agccggccgg cgtcatcggc
    19561 gggttgcttc aaaagatgaa acgtgaggcg ctgcgcggtt ttgcgtccga tcccggggag
    19621 cttggccagc tccgtcacga gatcttcaat ggctgacacg gtgccctacg acggcagctg
    19681 gaacggaaac ggcagccctg gagccagttt cttgacttcg gcctgcgcca gctcggccgc
    19741 ccgtttctgc gcctccgaca ccgccgcgag cacgagatct tcgagcatct cgacgtcgcc
    19801 ctgcttcacg gcttcggggt cgatccggac cgcccggatc cgcccctgcc catcggcagt
    19861 gacctgcacg attccgccac ccgctgaagc cgtcaccgtc tgctgagcga gctgagtctg
    19921 tatctccgac aaccggccct gcgcctgctg cgcgatctgg agcaggctgg ataaatccgc
    19981 catacgtcga aagttacccc tcgtctaata gtgttgcaag ctattccagt atctcgagat
    20041 ccagcgcgtc caccgccgcc gaaagcgtcg gatccttcgc ccgcagcacc cgcagacgtt
    20101 ccgcgttggc agtggcctcg gtcaaacgtg cggggcctgc cccctggttc cccaccggcg
    20161 ccggggcggc gacggactcc agctcaatgc ggaccgggcc ggtgaagtgg ggctccagaa
    20221 ggcgggtaat cgattcgcgc ttccgctcga tgccgtcctt gtgagcggcg gcgatccgca
    20281 gcacgaccac tccttcgtgc accgcgctga tttcgctgtc ggccagcagt gtgggcagga
    20341 gcggggaggt aaaacggctc tgctccacca ccccgggcca cagctggcgc gcgcgctcga
    20401 gtgtgaacgc cccgcgctcc gccgaggtgg agggtctggg tggcgccgag tcgcgaagct
    20461 caggtgcccg gcctgaaggg gccggtgccg gcgtgggtgc ggcgggagga ggagcaggag
    20521 gtccacgacg cggcgcggga gatgatgcgc cggggtcgcc cagggcacga atcacctccg
    20581 cgagctgcac cgtctggtcg agcaacgccc agcgcaagac cagcaactcc accaccaggc
    20641 gctggtttcc cgaatgccgg atctgccctt cggactcggc aagcatcgcg agaagccgca
    20701 ccacgtcacc cgtcgagaac cgcggagcgt acgccttcaa cgtgccggcc atgccctcgg
    20761 tcaggccctc cggctcgccg ccgaccgaca ggagcaacac cgcgcggagg gcttcccccg
    20821 cccccgcgac gaactcctcg agactcgccc ccgcttcgag caggtggtcc acgagcgcga
    20881 aggcgccctt cgcgtcgtgc tccgcgatga tgcgcaggag ctcgccgtat cgttcgtcgg
    20941 gaatcagccc caggacttcg cggacccgcg ccgccgtgac ggggcccgtc ccgaacgaca
    21001 ggacctgatc gagcaaggag agcgcgtcgc gcatgccgcc gtcggcgctc ttggcgatca
    21061 ggtgcagtgc gtcatcgtcc gccttcagct tttcctgcgc caggacgtgc ttcagccggt
    21121 ccacgatcgc atgcgggccg atgcggcgaa aatcgaatcg ctgcacccgc gacagcaccg
    21181 gagccgcggc ctgggcgatc ttctggggat ccgtagtcgc gaacacgaat accacacccg
    21241 tgggcggctc ttccagcacc ttgaggagtg cattccacgc ctcgcgggtg agcatatgcg
    21301 cttcgtcgac gatgtagacc ttgtgccggt tgtcggcgga ggccgcgtac atcgcgcgct
    21361 cgcggaggtc gcgggcgtca tccacgccgc ggttcgacgc ggcgtcgagc tccacgacgt
    21421 cgaggttggc ggcgcccgtc cagatgcggg agcaggactc gcacgtcccg cagggttcgc
    21481 cgtccttgcg gttcgggcaa ttcaaggcca tcgccaggac gcgcgcggcg gtggtcttcc
    21541 ctaccccccg gggacccgtg aacaggtagc cgtgggcggc gcgtccggag gccacggccc
    21601 cgcgcagggc ggcggcgacg tgatcctggg cgacgagatc agcgaattgc gtgggacgat
    21661 atttccgggc gagcgcgatc atctctgcgg ggaatctaac agggcgtggg gctcgaagct
    21721 gcggcggtcc atccggcccc accgtctcct cccgaccacg aactcccagt acgcctggag
    21781 cttgaacact gcgaggatct gccggtaccc cacgttttcg agcaatccga agagcatgag
    21841 cttgagcagg tcgctccagg agggatagcg gcggaagctg atctcctcca ggaacaccga
    21901 ggcgaccgac aggaacacgc ccaccagcac cgccacgagg aagaacttca cgaagaacgc
    21961 gccattcaag aggcccgtcg cggcacccac gatgacggtg aggtatccga gaccttcgat
    22021 caggggcccc agggcctcga acacgaggta gtacggcagc accaccatgc cgatgatgcc
    22081 gtacaccgga ttcaagaaca tccgccggtg ccgcaagagg ctctgcaaca tcccccggtg
    22141 ccagcgattg cgttggcggc ggaggtcccg ccaccgctcc ggcacctgcg tccagcacac
    22201 cgggtcggga acgaacacga tccggtacgg gaggcccctg gcgcggcggt actggtgcat
    22261 cgcgagaacg agctccaggt cctcggtgtc ggtggtcccg tcgtagccac cgacgtcgat
    22321 gaccgcctgc ttgcgataga cgccgaaggc gcccgagatg agcggcaagg cctgcagggc
    22381 gctccagccg agccgtccgc caaggaaggc gcggaggtat tcgacgacct ggaagatggg
    22441 gagcgcgcgc cgcggaatgg cgatccgggt cacccgccct tccttcaccg tgcagccgtt
    22501 cacgcagcgg acgatgccgc cgacggcgat ggtctcgtcg gggaactcca tgaacggctt
    22561 cacggccttc agcaacgcat cctcttcgat caccgaatcg gcgtccatgg agcagaagag
    22621 cggataccgc gacaggttga tgccgatgtt cagcgcgtca ggcttcccgc cgcgctcctt
    22681 gtccaccacg gtgagtccag gaacgagcgg ggatgcgtac acgccgcgca acggcttggt
    22741 cggcaggctg cgcttgaaca cgcggtccac ccgccgcagc tcgtaggcat cgagcaggcg
    22801 ctccaaggtg tggtcggtcg acccgtcgtt gatgacgatg atctcgaact gcggatagtt
    22861 gagcatccgc agggagcgaa cggtgtcgac gatcgtgttc tcttcgttgt gcgccggcac
    22921 caggagcgaa atcggcaaag tgaggtcgga ccggagaatc tgctggtagt cggagaagaa
    22981 cgtccgcttc agaaagcgga cgacctcgac cagcgagacg aggaagagga tcaggtacga
    23041 cagattgagg gcgaggaagt acaccaggat gaagttcccg aaccaggcga tggcgtgggc
    23101 gatccatccc aggatcatgc gctggccgcc tgtgtcttga gcaggcttcc cacttcccgg
    23161 cggacctccg gattcgggtg gcgcaccgcg atgtcgcgca gcagatcggt ccgcccgagc
    23221 gcgaccagcc gccgcagcac acccagcgcc tcccgccggt ccgcctcgtt gccctcgccc
    23281 aatcgaacga ggagcgcgtc gagcacgccg gcctcctgga gcatccaggc cgccttgtgc
    23341 gccgcgaagg cgtcgtcgcc gtcgagcgcc acgagcaggg cccggcgccc cgcctccccc
    23401 ttcccgcgca aggactcggc cgcgttggcg cgcacccacc attcgggatc ggagagcgcg
    23461 cgacgcagcg gttcgacgga gtcgtcaccg gcgatgcgtc ccagcgcctt cgccgccatg
    23521 gcgcgtaccg gccactccgg gtccgcgagc gcctcgatga gcgcctgcgt cacggcgggg
    23581 tgtccgatca atccgaggga attcgccgca cgcgcgcgcg cattgggatc ggaatccgcc
    23641 aggagaccgc ggagcagcgg gaccgagaga tgcgaccgca gccgccccag gatgtcgatc
    23701 gcgggcaccc gtgccgccgc cggcagccgc ggaaacgcct cgagcagggg ctcgacggcg
    23761 gaggggccga gcccggcgag tatgtcggcg attcggagcg tggcccaacg gtcggtggcc
    23821 ttgagagccg cgatcagcgt ctcgaccgcc tccagtcctc cgatggcacc cagcgccttg
    23881 gccgcgcgca ggcggacttc cggcacgggg tcgtccatca atttgacgag atcgtgtacc
    23941 gccggccgcg cgagcatccg tccgagcttc tcggcgcctt ccacccgcag ccaccaacgc
    24001 cggttcgaga gctggcggtg tgccagcgcg acgaatccca gctgctcgaa tgccagcgcc
    24061 aatcgggcct gcgagtgtcc cttgagcgat cggatgtggt cgaggagcag ccattcgacc
    24121 acgcgccgct cccaccgctt gagcggtgtg ggaacgaagt ccgtaatcgg cccttcggtg
    24181 gcgttggcgt aagcgacgat cgacgggagg aggagggcgc ggcgacggcg ctcgtagccg
    24241 tcacggagct cacgccaggc tttggtgacc agcactaccg aaaacaccag aaggaaaaga
    24301 ccgctcaggg cccccagcag aacgccgacg aactcctgca tccgttcagg accgcgccac
    24361 ccgggcgcgc gtcaggactt tccggacccg cgcgaggagc tcgcgggcat aaaacggctt
    24421 gatcaggaag tcctcggcgc cctgatcgag cgcgcgaacg atgtcgtctt ccccaccctg
    24481 ggtggagagc acgagcaccg ggatgtcacg ggtcgcctca tcgtcccgca ggtggcgaag
    24541 cacttcgaac ccggaggcat acggcatgat gagatcgagg aggatgacgt cgggaagctc
    24601 ggccttggcc gtcgtgacgc cggcggcccc atcggccgcg agcagcacct cgtatccgtc
    24661 agccttgagg accgcgctca agaactgacg gatggccgcg tcgtcgtcga tggacagaat
    24721 ccgtccaccg cgactggcgg tgtcgttgac ttcgggaagt aagggcgcca ttgagcgaaa
    24781 gtgccccagg caccccgcaa cgacattcgg cctcgttcag cgctgctccc tgcgggggcc
    24841 tgacgcggtt cacgagcgac tgccctgcgg acctggggcg gaggaaatct atccagcgtc
    24901 tctgacgtgc gtcagtgacc cacgataacc gtgaggttcg agaagatccc tgagccctga
    24961 acggcgagct gcgccgaccc ctggccccgg gcacggacgg acaaggtacc ggcgacagtg
    25021 tcgcctccta cccgctccac gcggataatc ccctcgtccc ccgagaggga ctgcaatccc
    25081 accaatgcca ccgcgttgcc gaactcgtcc gtagccacca cccgcaccgt ggcggtggtg
    25141 tccggggcga ggcgcagggt gtccccgatc gcattcggac ccaggtggaa ctcgagacgc
    25201 gaaggtgacg ccgccgccgg gtagagcgtc gccgagcgga aaatctgacc gatggcggct
    25261 cggatcac
//
LOCUS       DASUBP010000030         3896 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_504959, whole genome shotgun sequence.
ACCESSION   DASUBP010000030 DASUBP010000000
VERSION     DASUBP010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3896)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3896)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3896
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_504959"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..101)
                     /locus_tag="VFU75_01385"
     CDS             complement(<1..101)
                     /locus_tag="VFU75_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU5465510.1"
                     /translation="MAGKIRIRLKGFDHALIDQAAADIVRTAEKTGA"
     gene            complement(115..1314)
                     /gene="tuf"
                     /locus_tag="VFU75_01390"
     CDS             complement(115..1314)
                     /gene="tuf"
                     /locus_tag="VFU75_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU5465511.1"
                     /translation="MAKAKFERTKPHVNVGTIGHVDHGKTTLTAALTKVSADKGLATY
                     VPYDTVAKASESQGRRDATKILTIATSHVEYSTDERHYAHVDCPGHADYVKNMITGAA
                     QMDGAILVVSAVDGPMPQTREHILLARQVNVPAIVVFLNKCDLIDDPELLDLVELEVR
                     ELLSKYEFPGDEIPVIRGAALKAIEGDPKYVEKVWELLKALDKNIPIPVREVDKPFLM
                     PVEDVFSITGRGTVATGRIERGKIKVGEEVEMVGFGTDKKTVVTGVEMFRKLLDEGQA
                     GDNAGLLLRGIEKKDIERGMVLAKAGSITPHTAFDAEVYVLTKDEGGRHTPFFKGYRP
                     QFYFRTTDVTGTVELPAGVEMVMPGDNTKMAIELITPIAMEEGLRFAIREGGRTVGAG
                     VVTKIRK"
     gene            complement(1432..1504)
                     /locus_tag="VFU75_01395"
     tRNA            complement(1432..1504)
                     /locus_tag="VFU75_01395"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1469..1471),aa:Thr,seq:ggt)
     gene            complement(1518..1590)
                     /locus_tag="VFU75_01400"
     tRNA            complement(1518..1590)
                     /locus_tag="VFU75_01400"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1555..1557),aa:Gly,seq:tcc)
     gene            complement(1604..1686)
                     /locus_tag="VFU75_01405"
     tRNA            complement(1604..1686)
                     /locus_tag="VFU75_01405"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1650..1652),aa:Tyr,seq:gta)
     gene            complement(1774..1846)
                     /locus_tag="VFU75_01410"
     tRNA            complement(1774..1846)
                     /locus_tag="VFU75_01410"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1811..1813),aa:Thr,seq:tgt)
     gene            complement(1924..3597)
                     /locus_tag="VFU75_01415"
     CDS             complement(1924..3597)
                     /locus_tag="VFU75_01415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEU5465512.1"
                     /translation="MINPLLLAFTVALHAPADSIAIIPKPVVLVRHPGSFTLRPHTVI
                     MTSPELKPLGQLLVSYLRPATGVDFTLTTGNQIPRAAGPSPSIILLAIDSLQGGHGPE
                     GYSMDISSAEVAIQGATPAGVFYGIQSLRQLFPPDIFRRAPVGDVQWTIPCLSIVDYP
                     RFGWRGMHLDVSRHFMPKEFVEKYIDLIALYKFNRFHWHLTDDQGWRIEIKRYPRLTS
                     VGGWRAQTLVGREPDDTAGAKYDSTRHGGFYTQEDIREVVAYAAARYITVVPEIEMPG
                     HSQAAISAYPELGSTGQPVKVKEDWGVSPWILAPSDSSIAFMENVLTEVMQLFPSKWI
                     HTGGDEAVKDQWKASPLAQQRIKALGLKNEDALQGWFTAQIDAFLHSHGRTLIGWDEI
                     LDGGLSPNAVVMSWRGIDGGIAAAQAGHDAVMAPGQYTYFDHYQSGDRDTEPLAIGGF
                     LPLDSVYAYNPVPKALTADQAQHILGAQGQVWTEYIPDPKHVEYMAFPRVEALAEVLW
                     DPEGTTNFQDFFSRLTAVDVHRLDALDVRYRNPAWAVALPPRSGRRGRR"
     gene            3658..>3896
                     /locus_tag="VFU75_01420"
     CDS             3658..>3896
                     /locus_tag="VFU75_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465513.1"
                     /translation="MAQAHLAGIDYAIMAVYFAFVLGIGIVLRRSVHTGNDFFLAGRA
                     VPAWIAGLAFLSANLGAQEVIGMGASGAKYGVATS"
BASE COUNT          653 a         1347 c         1186 g          710 t
ORIGIN      
        1 tgcgcaccgg tcttctcggc cgtgcggacg atgtccgcgg cggcctgatc gatcagcgcg
       61 tggtcgaaac ccttgagacg aatccggatc tttccggcca ttgggacttc tccgttactt
      121 cctgatcttg gtgacgacgc ccgcgccgac cgtccggcca ccttcccgga tcgcgaaccg
      181 caggccctct tccatcgcga tcggcgtgat cagctcgatc gccatcttcg tgttgtcccc
      241 cggcatcacc atctcgaccc ccgccggcag ctccaccgtc cccgtcacgt ccgtcgtccg
      301 gaagtagaac tggggccggt agcccttgaa gaacggcgtg tggcgcccgc cctcgtcctt
      361 cgtcaggacg tagacctcgg cgtcaaaggc cgtgtgcggc gtgatcgagc ccgccttggc
      421 cagcaccatc ccgcgctcga tgtccttctt ctcgatgccg cggagcagga ggccggcatt
      481 gtcgcccgcc tgcccctcgt ccaggagctt ccggaacatc tcgaccccgg tcacgaccgt
      541 cttcttgtcg gtcccgaagc ccaccatctc cacttcctcg ccgaccttga tcttgccgcg
      601 ctcgatgcgg cccgtggcca ccgtaccgcg gcccgtgatc gagaacacgt cctcgacggg
      661 catcaggaac ggcttgtcca cctcgcgcac cgggatcggg atgttcttgt cgagggcctt
      721 caggagctcc cagaccttct ccacgtactt gggatcccct tcgatggcct tcagggccgc
      781 gccccggatc accgggattt cgtcgcccgg gaactcgtac ttggacagga gctcccgcac
      841 ctcgagctcc accagatcca ggagctcggg gtcgtcgatc aggtcgcact tgttcaagaa
      901 cacgacgatc gccggcacgt tcacctggcg ggcgagcagg atgtgctcgc gcgtctgcgg
      961 catcgggccg tcgaccgcac tcaccacgag gatcgcgccg tccatctgcg cggcccccgt
     1021 gatcatgttc ttcacgtagt cggcgtgccc cgggcagtcg acgtgcgcgt agtggcgctc
     1081 atccgtcgaa tactccacgt ggctcgtcgc aatcgtcagg atcttggtcg cgtcgcggcg
     1141 cccctgggac tccgacgcct tggcgacggt gtcgtagggc acgtacgtcg caagcccctt
     1201 gtcggccgag accttggtca aggccgcggt caaggtggtc ttgccatggt ccacgtgccc
     1261 gatcgtcccg acgtttacgt gcggcttcgt ccgctcgaac tttgccttcg ccatagaccc
     1321 ctcaaaaaac gttgaacaga atcgtcaagc agaaccgtcg agcacaaccg taaccgacgc
     1381 cgtcgcttga cgcatttgct agacgccttt cttgacgtct ttgcttttcc aagctcgcga
     1441 ccgggattga accggtgacc tcgtccttac caaggacgcg ctctaccgac tgagctacgc
     1501 gagcgactca ctttcagagc gggcgacggg actcgaaccc gcgaccctca gcttggaagg
     1561 ctgatgctct accaactgag ctacacccgc gcttgcgcct cgatggtggg ggaaggattc
     1621 gaaccttcgt aggctgacgc cggcagattt acagtctgct ccctttggcc gctcgggcac
     1681 cccaccgacg tcccttcccc tacagactcc cactgacgac gcaccccgcg ttgcggtacc
     1741 cgcggggtgt agaccagagc cttcggcaac gtgagctgac gaagggactc gaacccttaa
     1801 ccggcggttt acaaaaccgc tgctctacca gttgagctac gtcagcgagc cggtcgtgaa
     1861 actttccaga acggaagact aacgggggtg ggggcctgat gcaagggatg ggccagaaag
     1921 gagttaccgc cgcccacgcc ggcccgaccg cgggggaagc gctacggccc aggccggatt
     1981 ccggtagcgg acatccaggg cgtccagccg gtggacatcc acggccgtca ggcgggagaa
     2041 gaagtcctga aagttcgtgg tcccctcagg gtcccagagc acctccgcca gggcctcgac
     2101 ccgagggaac gccatgtatt ccacgtgctt ggggtcgggg atgtactccg tccacacctg
     2161 cccttgagcc cccaggatgt gctgcgcctg atccgcggta agggccttcg gcaccgggtt
     2221 gtaggcgtag accgagtcca aaggtaggaa gcccccgatc gcgagcggct cggtgtcccg
     2281 gtccccggac tgatagtgat cgaagtaggt gtactgcccc ggcgccatta cggcgtcatg
     2341 ccccgcctgg gccgccgcga tcccgccatc gatgccccgc cagctcatca ccacggcgtt
     2401 gggcgagaga ccgccgtcca ggatttcatc ccagccgatc agcgtccggc catgcgagtg
     2461 cagaaacgca tcgatctgcg cggtgaacca cccctgcagc gcgtcttcat tcttcaaacc
     2521 gagggccttg atgcgctgct gagcgagcgg gctggccttc cattggtcct tcaccgcctc
     2581 gtccccgccg gtgtggatcc acttgctcgg gaacagctgc atcacctcag tgaggacgtt
     2641 ctccatgaac gcgatgctcg aatcggacgg cgcgaggatc cacgggctca cgccccaatc
     2701 ctcttttacc ttcaccggct gacccgtgct tcccagctcc ggatacgccg agatcgccgc
     2761 ttgcgagtgg cccggcattt cgatttccgg gacgacggtg atgtaccgcg ccgcggcata
     2821 cgccacgacc tcgcggatgt cctcctgcgt atagaagccg ccatgccgcg tggagtcata
     2881 cttcgcgccc gcggtgtcgt cgggctcgcg gcccaccagc gtctgcgcgc gccatccgcc
     2941 caccgacgta agcctgggat agcgcttgat ctcgatccgc cagccctgat cgtcggtcag
     3001 atgccaatga aagcgattga acttgtagag ggcaatcagg tcgatgtact tctcgacgaa
     3061 ttccttcggc atgaagtgcc gcgagacgtc gaggtgcatc ccccgccagc cgaaccgcgg
     3121 gtagtccacg atcgacaggc acgggatggt ccactgcacg tctccgaccg gggcccgccg
     3181 gaaaatgtcc ggtgggaaca gctgccggag tgactggatc ccgtagaaca cccccgccgg
     3241 cgtcgccccc tgaatggcga cctcggccga cgagatgtcc atcgaatacc cctcaggtcc
     3301 gtgccccccc tgcagggagt cgatggcgag caggatgatg gacgggctcg gccccgcggc
     3361 gcgcgggatc tgattgcccg tggtgagggt gaagtccacc ccggtcgccg gacggagata
     3421 cgagacgagc agctgcccga gcggcttcag ctccgggctg gtcatgatga cggtatgcgg
     3481 gcgcagcgtg aaggagcccg gatgccgcac cagcaccacg ggcttcggga tgatggcgat
     3541 ggagtcggcg ggtgcgtgca gagccacggt gaaggcgagt aatagaggat tgatcatcgc
     3601 gttccaagat cgtgccccgc ttgccgcctc cgcaagcggg ctgcacattc gcaccccatg
     3661 gctcaggccc atctcgccgg catcgactac gccatcatgg cggtctactt cgcgttcgtg
     3721 ctggggatcg gcatcgtcct ccgccgctcc gtccacacgg gcaacgactt cttcctcgcc
     3781 ggacgggccg tccccgcctg gatcgccgga ctcgcgttcc tctcggcgaa cctcggcgcc
     3841 caggaagtga tcgggatggg agcctcgggc gccaagtacg gcgtcgcgac cagcca
//
LOCUS       DASUBP010000031         5119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1050030, whole genome shotgun sequence.
ACCESSION   DASUBP010000031 DASUBP010000000
VERSION     DASUBP010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5119
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1050030"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="VFU75_01425"
     CDS             complement(<1..722)
                     /locus_tag="VFU75_01425"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HEU5465514.1"
                     /translation="MSTLETPLTRDAGIAVPLICGAMYPCSNPELVAAVSDAGGIGIT
                     QPLSMVYVHGHEFREGLRLIRRLTPKPIGMNVLIEQSSRAYLDKMRRWLDVALEEGVR
                     FFVTSLGNPRWVVDRVRPVGGVVYHDVTERKWALKALDGGVKGLIGVNNRAGGHAGAR
                     SAEELLDELGDLGVPLVCAGGMGAPADFARVLRLGYAGAQLGTRFIPTPECHAHQDYK
                     QAIVDADEQDIVLTERVTGVPLA"
     gene            complement(719..1204)
                     /locus_tag="VFU75_01430"
     CDS             complement(719..1204)
                     /locus_tag="VFU75_01430"
                     /inference="COORDINATES: protein motif:HMM:NF025892.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold domain-containing protein"
                     /protein_id="HEU5465515.1"
                     /translation="MPNPASPGDTLRTAWRRLRPLPGGRRIFSWLIRWMVPYSGTTRP
                     YVEELAPGFARVRLSDRRGVRNHLHSVHAVALANVAELASGLAMTTALPPGVRGIVVR
                     LSIDYTKKARGTLTASARVTVPAIVAAEDHDFESVVTDETGDVVARATVRWRLAPARP
                     A"
     gene            1252..2022
                     /locus_tag="VFU75_01435"
     CDS             1252..2022
                     /locus_tag="VFU75_01435"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5465516.1"
                     /translation="MTRIAGKTILITGASSGIGAACARRFAAEGANLVLWARRRDRLE
                     VMAAELGKAHSVKVRVDEVDVRDRVSVEKAAKALLAQGSAPDVLINNAGLASGLSLVQ
                     DGDLEDWDRMIDTNVKGLLFVTRALLPGMIARGVGHVINIGSTAGHQVYQKGNVYNAT
                     KFAVKALTEGINLDVAGTPIRVSSVDPGFVETEFSLVRFHGDEARAKSTYQGFRALTG
                     DDVADTISYVVNLPPHVNILDLILVPSAQRSVHLVDRT"
     gene            complement(2244..2987)
                     /locus_tag="VFU75_01440"
     CDS             complement(2244..2987)
                     /locus_tag="VFU75_01440"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HEU5465517.1"
                     /translation="MTTTATAPARYWQLSQTPRYSLLFALPLFIGYEVLQLAVSGGPG
                     GDIRNGADVILQSLFAELMGRRGPMVFEIALIVLGLALVAHDMRRHGRHLSRMVFFGM
                     SLETLALAILCGVLVGGITSHLFGAMGLSIGQIERTPLPVRLMLSLGAGLYEELLFRV
                     IIVGVLAWGGHRLLGWSARLSGIVAVTVGALAFSAVHYVGTYGDQLTLYSFVYRTLAG
                     VFFSALYLLRGFGITAWTHALYDVFVLVA"
     gene            3060..>5119
                     /locus_tag="VFU75_01445"
     CDS             3060..>5119
                     /locus_tag="VFU75_01445"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEU5465518.1"
                     /translation="MLLLLFAVGLAAPPQHPDTATYLDATARVLVERARAARSDQAAG
                     ILAYSATAKQRIYFGMRALRRDRVFYHSETAARVHWHRVGHDSVEVLGAREGIPIALP
                     HDEVPDDLRHDVPDLLWSPSSDRMSFGDGDNFVHHPLGDSAAADYRYQSGDTTSIILP
                     NGVEIRLCELKIIPRRPDPHLMAGSIWIEAKGYSMVRALFQLARPWDLELDLDRESGE
                     NVSSHIPGFLTPIRAELKYATVEFSLWQNHWWVPRLQSIDGTGTAGSIASFPLRFEIA
                     YSDYQLDGDTTEHTPPPVARLDSAAADSLWKRCSNDSTGSISCHCHDHQCHEVTVVLP
                     ADTLALLTSPDLPPSFVTDESALTTRADLDNILADLHLLPRSSFENHAQLRALWEAPG
                     LLRYNRVEALSLGARADVPMGPLAFDGTFRVATATGQPDVDLGVTRKTQDLTARFGAY
                     RRLTAVDPDAHSLGFGNSVQAVVLGRDDGDYFRTAGVDIDVKPAITESQVYEWRLYAE
                     HQFVAPLGTARASLGHLLDGGTFFRPQIPAQRADQIGSALRLRGDLPLNEGRASLGAN
                     LDMDLSVGTYNFARLALTGRAAAPLPGPFQGAVEISGGASDGKVPLQSHWFLGGPTTV
                     RGYGGDAANGTAFWRARAELGFGIPGARLALFSDAGWAGVGRFAFTAQPLISAGLGAS
                     FLDGL"
BASE COUNT          873 a         1732 c         1691 g          823 t
ORIGIN      
        1 gccaggggca cgcccgtgac ccgctcggtg agcacgatgt cctgctcgtc ggcgtccacg
       61 atggcctgct tgtagtcctg gtgggcgtgg cactccggtg tgggaatgaa acgcgtgccc
      121 agttgtgccc ccgcgtaccc gagccggagg acccgcgcga agtcggccgg ggctcccatg
      181 ccgcccgcac agaccagcgg cacccccaga tcgcccagct cgtcgaggag ctcctcggcg
      241 ctgcgtgcgc ccgcgtgccc gccagcccga ttgttcacgc cgatgagccc cttcaccccg
      301 ccgtcgagcg ctttcagcgc ccacttccgc tccgtcacgt cgtgatagac cacgccgccc
      361 accggacgaa cccggtccac cacccatcgg ggattcccga gggacgtgac gaagaatcgc
      421 acgccttcct cgagcgccac gtccagccag cggcgcatct tgtccaggta cgcgcgagat
      481 gactgctcga tgaggacatt catgccgatg ggtttcggcg tcaggcgccg gatgagccgg
      541 agcccctccc ggaactcgtg gccgtggacg tagaccatcg acagcggctg ggttatcccg
      601 atgccgccgg cgtccgacac cgccgccacg agctcggggt tcgagcaggg gtacatcgcg
      661 ccgcagatga ggggcacggc gatccccgcg tcgcgcgtca gcggcgtttc gagagtgctc
      721 atgcggggcg cgccggggcc agacgccacc gcaccgtggc ccgggccacc acgtcgccgg
      781 tctcgtcggt caccaccgat tcgaagtcgt ggtcctcagc cgcgacgatg gccgggaccg
      841 tcacccgcgc cgatgcggtc agggtgccac gagccttctt ggtgtagtcg atggacaagc
      901 ggaccacgat gccgcgaaca ccgggaggga gggcggtcgt catggccagg ccgctcgcca
      961 gctcggcgac attggccaga gccaccgcgt gcaccgagtg gaggtggttg cggaccccgc
     1021 gccggtctga caaccgcacc cgggcgaagc ccggtgccag ctcctcgaca tagggtcgtg
     1081 tggtgcccga gtagggcacc atccagcgga tcagccagct gaagatccgc cgtccgccgg
     1141 gcagggggcg aaggcgacgc caggcggtgc gcagcgtgtc gccgggggag gcagggttcg
     1201 gcatggaggg atggtagtga gccgagtctc gaaccgctac tacatttccg catgactaga
     1261 atcgccggga aaaccatcct catcaccggc gcctcctccg ggatcggcgc cgcctgcgcc
     1321 cgtcggttcg ccgccgaggg tgccaacctc gtcctgtggg cacgccgtcg cgaccggctc
     1381 gaggtcatgg ccgccgagtt gggcaaggcc catagcgtca aagttcgggt cgacgaggtc
     1441 gacgtgcggg accgggtctc ggtcgagaag gcggcgaagg cgctgctcgc tcagggctcc
     1501 gcgcccgacg tactgatcaa caatgccgga ctcgcttccg ggttgagtct ggtgcaggac
     1561 ggcgatctcg aagactggga ccggatgatc gacacgaacg tgaagggtct cctgttcgtg
     1621 acgcgcgcgc tcttgcccgg catgatcgcc cgcggggtgg ggcacgtgat caacatcggg
     1681 agtaccgccg ggcaccaggt ctatcagaag ggcaacgtct acaacgcgac gaaattcgcc
     1741 gtgaaggccc tgacggaggg gatcaatctc gacgtcgccg gcacgcccat ccgcgtctcc
     1801 agcgtagacc cggggttcgt cgaaaccgag ttttcattgg tgcggtttca tggagatgaa
     1861 gcgcgggcga agagtaccta tcaaggcttt cgcgcgctca ccggcgacga tgtggcggac
     1921 accatttcgt acgtcgtgaa cctgccgccc cacgtgaaca ttttggatct catcctggtc
     1981 ccatcggcac agagaagtgt gcacctggta gatcggacat agcatccgat cttccactgt
     2041 taattctctc cctaaacaaa tgagcacggc tgtcatcctg agcgagcgca gcgagcgaag
     2101 gacccgcttc gtcttgcggt gcacgatatc gcccggtgaa agcaggtcct tcgctccggg
     2161 cgcttcgcac ccttcgctca ggatgacatg cgcttcgctt tgttgagaaa tgaaatggaa
     2221 gaatggcggg tgctagcgcc gagttacgcc acaagcacga acacgtcgta cagggcgtgc
     2281 gtccatgcgg tgatcccgaa gcctcggagc agatagagcg ctgagaagaa cacccccgcc
     2341 agtgttcggt acacgaacga gtacagcgtc agctggtccc cgtaggtccc cacgtagtga
     2401 acggctgaaa aggccagcgc tcccactgtc acggcgacga ttcccgatag ccgggcactc
     2461 caaccgagga gccggtgacc tccccaggcg aggacgccga cgatgatgac ccggaagagc
     2521 agctcctcgt agagaccggc gcccagggag agcatgagcc gcaccgggag aggggtccgc
     2581 tcgatctggc cgatggagag ccccatggcg ccgaagagat gagaggtgat ccccccgacc
     2641 agaacgccgc agagaatcgc gagtgccagg gtttcgagag acatgccgaa gaagaccatc
     2701 cgcgacaggt gccggccatg gcgccgcatg tcgtgtgcaa ccagggccaa tcccagcacg
     2761 atcaacgcga tttcgaagac catggggccg cgccgtccca tcagctcggc gaacaggctc
     2821 tggaggatga cgtccgcgcc attccggata tcccccccgg gacctcccga caccgcgagc
     2881 tgcaggacct catatccaat gaagagagga agcgcgaaca gcaggctgta gcggggcgtc
     2941 tgggagagct gccagtagcg cgctggagcg gttgcggtgg tcgtcatggc ctttcaaggt
     3001 aaacgggtac gggtgcaact ttgtgtcacg gaaggtcgta gagccatttc ggtctcccaa
     3061 tgcttcttct cctcttcgcg gtcggcctcg cggccccccc ccagcacccc gacaccgcga
     3121 cctacctgga cgcgactgct cgggtactcg tcgaacgcgc ccgcgcggcc cggagtgacc
     3181 aggccgcggg cattctcgcc tacagcgcca ccgccaagca gcgaatctac ttcggcatgc
     3241 gggcgctccg ccgggaccgc gttttctatc acagcgagac cgccgctcga gtgcattggc
     3301 accgggtggg acatgactcg gtcgaagtcc tgggcgcgcg ggaggggata ccgatcgccc
     3361 tgccgcacga cgaggtcccc gacgacctcc ggcacgacgt ccccgatctg ctgtggagcc
     3421 cctcgtcgga ccgcatgagt ttcggcgacg gggacaattt cgtgcaccac ccgctggggg
     3481 acagcgccgc ggcggactac cggtaccagt cgggggacac gacgtcgatc attctgccga
     3541 atggcgtcga gatccggctc tgtgaactga aaatcattcc gcgccggccc gacccgcacc
     3601 tgatggcggg atccatctgg atcgaggcca aggggtactc catggtacgg gcgctctttc
     3661 aactcgcgcg tccctgggat ctggagctcg atctggatcg cgagtcgggg gagaacgtga
     3721 gcagccacat cccggggttc ctcacgccca ttcgcgccga gctgaagtac gcgacggtcg
     3781 agttctccct ctggcagaat cactggtggg tgccccgcct tcagtcgatc gacggaaccg
     3841 ggacggccgg atccatcgcc tcgtttccgc tgcgtttcga gatcgcctat tcggactacc
     3901 agctggacgg cgacacgacg gagcacacgc ctcctcccgt cgccaggctg gactcggcgg
     3961 ccgccgactc gctctggaag cgctgcagca atgactcgac cgggagcatc tcctgtcatt
     4021 gccacgacca ccaatgccac gaggtgacgg tcgtcttgcc ggccgacacc ctcgcgttgc
     4081 tgacgagtcc cgacctcccg ccgtcgtttg tgacggatga atcggcgctg acgacgcgcg
     4141 ccgatctcga caacattctg gcggacctgc acctgctccc gcgttccagt ttcgagaatc
     4201 acgcgcagct cagggcgctc tgggaggccc ccgggctgct ccgctacaac cgcgtcgaag
     4261 ccctctccct cggcgcccgc gccgacgtcc cgatgggtcc gctggcgttc gacggcacct
     4321 tccgcgtggc cacggccacg ggacagccgg atgtcgacct cggggtcacg aggaagacgc
     4381 aggatctgac cgcgcgcttc ggcgcgtacc ggcggctcac cgccgtcgat cccgacgcgc
     4441 attccctggg gttcggcaat tccgtgcagg ccgtggtgct gggccgggat gacggggact
     4501 acttccgcac cgcgggtgtg gacatcgacg tgaagcccgc aatcacggaa tcgcaagtat
     4561 acgaatggcg cctctatgcg gagcaccagt tcgtggcgcc gttgggcacc gcgagggcga
     4621 gcctggggca cctcctcgac gggggcacgt tcttccggcc gcagattccg gcgcaacgcg
     4681 ccgaccagat cggcagcgcg ctccggctgc gcggcgatct cccgctcaat gaagggcggg
     4741 cctcgctcgg cgccaacctc gacatggacc tgtcggtggg gacctacaac ttcgcgcggc
     4801 tggcactgac cggccgggcc gcggcgccgc tgcccggtcc gttccaggga gccgtcgaaa
     4861 tctccggcgg cgcatcagac gggaaagtgc ctctgcagag ccactggttc ctcggtggcc
     4921 cgacgaccgt gcgtggctac ggcggggatg ccgcgaacgg cacggcgttc tggcgcgccc
     4981 gtgccgagct ggggtttggc attccgggcg cgcgtctggc gctgttctcc gacgccggtt
     5041 gggccggcgt gggccgcttc gccttcaccg cccagcccct catctccgcg gggctgggcg
     5101 cgagcttcct cgacggact
//
LOCUS       DASUBP010000032        51425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_654821, whole genome shotgun sequence.
ACCESSION   DASUBP010000032 DASUBP010000000
VERSION     DASUBP010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 51425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51425
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_654821"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            88..615
                     /locus_tag="VFU75_01450"
     CDS             88..615
                     /locus_tag="VFU75_01450"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5465519.1"
                     /translation="MIARVRAGDPAAERALYDAHVDRVYRLSYRMAGDDDLARDFTQE
                     TFIRAFERLDTFRGESAFSTWLHSIATTVALNGLRKLKRFRKRETDIEEADGVTASRA
                     NAEPDLKKRLAQAIDALPEGYRTVFLLHDVEGYTHEEIGAALAIESGTSKAQLSRARA
                     KLRIALADFAGEWIG"
     gene            612..1229
                     /locus_tag="VFU75_01455"
     CDS             612..1229
                     /locus_tag="VFU75_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465520.1"
                     /translation="MNENREDRFEQELKRMAQDYHRPPETPRDEMWARITAERARRRE
                     SMKVVPLRRWMAWSLAAAAVLVLGVAIGRWSMNVKEKNPGPRNEEMAYRAAAAPYLTR
                     TEALLTSFRAESRNGKVDADFAVQAQDLLVTTRLMLNSPAARDPRLASLLQDLETMLV
                     QIAQLPAGNDRNDVDLINQGLDQRSVMLRLRTIGPTPGTSHRGAL"
     gene            1229..2773
                     /locus_tag="VFU75_01460"
     CDS             1229..2773
                     /locus_tag="VFU75_01460"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU5465521.1"
                     /translation="MISLHLLLTAATFIQTPVGAPVPPVPLTAVAAVAPALAQARAAL
                     DIARAQVAPLSRVYPSIAMAYAGHARAYAALARDQVRAARWATRAPVGEYTDDPGDSL
                     YNAARSLLNRARYNEAASAFADLAKRYPKSRYAPDSYYWQAFSLYKTGDASKLKQARS
                     VLEYQQQHFPDAATHGDADALYAQIQGALARQGDGPAGEWVQKHASQADAERPTTSGS
                     CASSDDDDDDPRIAALNALLQMDADRAVPVLKKVLERRDPCSVSLRRKAIFIISQQHT
                     AETEDILLGAARNDPDEDVREQAVFWLSQVGTAKATAALDSILLNSTNTELQDKAIFA
                     LSQIDSPEASKAIRDYAERPGAPVEARGKAIFWLSQQHDGANAAYLRALYGRLTDDDL
                     KDKTIFAISQLGGADNTNWLVDLAANKQESVEMRKKALFWAGQSNEVSLDRLTGLYDR
                     MDDRDMKEQMIFVYSQRHEGAALDKLIDIAKHDTDPELRKKAIFWIGQSHDPKAAQYL
                     QEVISQ"
     gene            2770..3573
                     /locus_tag="VFU75_01465"
     CDS             2770..3573
                     /locus_tag="VFU75_01465"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU5465522.1"
                     /translation="MIRALAALGAALLVGSAAAAQGISHRVSAAPDGKVRMTFASRPG
                     VCGNGSNDISMDCEDGNCGNHNTYSRDDHDVDVTTCPCEEGPVRLVLRVSGGKVTRIR
                     AYVGGEWKPAEAGTTDLGVVGDKDAVAYLLDLARTSSSHIGEEAIFPATLADSVNVWP
                     DLFKIARSDEVPAQTRKSAVFWIGQAAGETVTKGLDSLAQGSDVDREVRESAVFALSQ
                     QRNDVGVPALISIARTNKDPDIRKKAIFWLGQSNDPRALALFEELLTRK"
     gene            3704..6391
                     /locus_tag="VFU75_01470"
     CDS             3704..6391
                     /locus_tag="VFU75_01470"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEU5465523.1"
                     /translation="MLALLLLLQGPQTQKPTLPSSPVDSSALRHSNGLVPPMAFGVRV
                     QQAPAMDGKLDDPLWATATAVTDFRQVDPHEGQPASERTEVRVLYTDDALYIGIRAWD
                     DSASKIAHHLGRRDTFTQSDDFRVLIDSYHDHRTAFRFDITPTGVKGDIQFGDDGNFA
                     DQSWDPVWTAVTSIDSLGWTAEEKIPFSQLRFSRATDQIWGIRFVRTILRKNEFDLFP
                     FVGKTENGFVSRFAHLGGLKNIPAPRRLELLPYTSGSGTFQPQGPVGNPFSKTSAYSG
                     NAGLDLKYGVSSFLTLDATINPDFGQVELDPAFVNLTAFEQFLPEHRPFFVEGADIFN
                     FGGGSGGLANFGSTPRFFYSRRIGRRPQGDTYSSGDFVDYPANTSILGAAKLTGKTPS
                     GWSVGFLDAVTSEEKASVLDTTSHLTARDLVEPLSNFMVGRVRRTLNGGRSSYGGMIT
                     AVNRDLAVPSLRGLNSGAYTFGVDTKQLWSRNTYALAADVGGSYITGDTTAIQDAQFS
                     SGRYYQRPDARAFHYDPTRTSLAGFNGNVYLDKLAGTWLWGSAISTSSPGFEVNDLGF
                     QPRVDRISGYAYLARHQTTPGPLFRESWAVLTVGPSWNYDGNPIQKAVTASYFGNLNN
                     FWFLQVQGNYGAQVVDDRLTRGGPLALAPAFWNGFAMIQTNQSKAFTAMVMGSYNKDN
                     AGSWQFNVSPQLGYRASSALNFSITPNFTVGPTAAQYVQAVTDSTAGSTFGSRYVFAQ
                     LFQHEFDVTLRVSATVSPNLSLEVFAQPFSFTGSYGGFKELAQPKTFAFNRYGENGTS
                     INFNPTDSLYHVFPNAAQPADSFTFANPDFRTRSVRINAVLRWEYRPGSTLFIVWTQS
                     RGGMFSDPTFDIGRDFDHQLFQDPPTNVLLVKLNYWMSL"
     gene            6515..7111
                     /locus_tag="VFU75_01475"
     CDS             6515..7111
                     /locus_tag="VFU75_01475"
                     /inference="COORDINATES: protein motif:HMM:NF014724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEU5465524.1"
                     /translation="MPSARHAFKISLSLALLLAAGWAAVVIDVLITGSREGAAPADAI
                     AVLGAAQYNGKPSPVFKARLEKAAWLYRSGKAPVILVTGGIGNRDTVSEAEVGRRYLT
                     HIGLPGDSVIALPAAATTVSSIGQLGAWFKDRPSKRVDLVSDGFHLLRLRILANRQGL
                     VPFTSPAKASPISASLRTNAAYVAAEGWKVPVAWLFYR"
     gene            7127..7666
                     /locus_tag="VFU75_01480"
     CDS             7127..7666
                     /locus_tag="VFU75_01480"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEU5465525.1"
                     /translation="MQVGTRRVHTGKVIDLDVDTVKYPDGSSGELEMIRHPGASAVVP
                     VLSDPDGPDPTILLIKQYRYATGGMLWEIPAGRLGKGEAPVDCARRELLEETGMHAGK
                     IEPLIPIWTTPGFTDERIHLFWARDLTPGEMAREADEFIELAPRPLSQVLTAIRDGEI
                     CDAKTVTAILLVAGFVFGL"
     gene            7835..8479
                     /locus_tag="VFU75_01485"
     CDS             7835..8479
                     /locus_tag="VFU75_01485"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5465526.1"
                     /translation="MRAILKRKTTPVATDRAIEREKLHELDDGHVVSRHLAGDPQAFG
                     TLVDRYQVRLLNFVNRTIGDRERAEDLVQEVFIRVFRHIHRFDQTKKFSTWIYTIASN
                     LAKNELRNRSRNPLVLFQTIKKNWDADHRPLQFEDTTARPDDLYRKRYLKDAVDSCVK
                     ELPEHHRAVFVLRELEGRSYQEIAEITGCNLGTVKSRLNRARNTFAHLIGPQLD"
     gene            8529..9023
                     /locus_tag="VFU75_01490"
     CDS             8529..9023
                     /locus_tag="VFU75_01490"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEU5465527.1"
                     /translation="MECAEFLDRYTDYRDGVVTTPRDRRRFERHLAHCETCRSYDTAV
                     RRGVLALQGVETLTPSERFRARLDARLRREALTMAGPRISPRIGAAAALLAAVGIALV
                     ALQLAHRPAPLVAHALPPVPFPKPVAQAGVPFVTFQDPRAMTAAGNPMPYGSALVEPV
                     SAHH"
     gene            9062..10117
                     /gene="holA"
                     /locus_tag="VFU75_01495"
     CDS             9062..10117
                     /gene="holA"
                     /locus_tag="VFU75_01495"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEU5465528.1"
                     /translation="MATLSFNALLGGGKPGSLDPVYYLHGEEEILKEEAVRLLLDRAV
                     DPSLRDFNVDARSAPELDPAALRTLVDTPPMMADRRVVILRSVEGFRAKSKTRDELVR
                     YLAHPNPSTTLILVQGGDEAPDAGLTAHATTVAALRLPPDRVTRWITHRAGQIGITID
                     TAAAELLGRATAHDLAAVSQELDKLAAIAGTRTVTEADVTAAVGVRHGETLDDLITAT
                     LERRVGDAARTLGPVLAQSGMSGVKLVSTLGTGLVGLAAARAELDRGTAPGRLEYTIL
                     QRLIASRPAGLGAGYKDVAARWAKWVRGWTAPELSRAIRRALAADRALKATQLTDEYG
                     IVLDLILGLARASREAA"
     gene            10222..11016
                     /locus_tag="VFU75_01500"
     CDS             10222..11016
                     /locus_tag="VFU75_01500"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEU5465529.1"
                     /translation="MKSAVDLAQSGQVDSARSMVRKLLATMSPHDSAYPQALYTQGML
                     AADAPTVTSNLQRVVVEYGWSPWAANALMRLAQLAYTQGDPASAIQSVQRLRRDYPDS
                     PVKPRADFWGAKADFDLRNETEGCELIREALAGAGTDVEFKNQVSFYAARCGATPAPT
                     PTASAPPAAATPAAPTSPPRDTSPATSGKTTYAVQVLAVKSAADVDALLTRLKVMGYE
                     GHVVPRDSTGLIKVRVGRYATRPEALRAQSQLKARLGGQPFVVTEP"
     gene            11025..13625
                     /gene="mutS"
                     /locus_tag="VFU75_01505"
     CDS             11025..13625
                     /gene="mutS"
                     /locus_tag="VFU75_01505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEU5465530.1"
                     /translation="MASAPPPADTPLMQQYREIKSRHPNTILFFRMGDFYEMFEDDAK
                     LGARVLGLTLTSRNNGGASEVPLAGVPVKAATEYLRKLVSAGHRVAVCEQVEDPKLAK
                     GLVRREVIETITPGSVLAEDWIATGKNNFLAALLPQESVVGLAALDLTTGEFVWETLL
                     PEDVAAGLARYEPSELVVPAGGAGPRELTGTRTEREAWEFDPDLAREDLQRVFGLASL
                     DGLGVESTDRAALGAAGALLRYARELKPGGLPHLARPRLVRRGAVLPLDEMTRRNLEL
                     IEPLRPGATGTTLLEVLDRTLTPMGARLLRRWLLAPLVDAGAVNQRLDAVQVLVMDPR
                     GRDRLREALDGVRDLERLAGRAAVGRATPRELGALRDSIRKLPDVRAGLDGLEARDHS
                     ALLEEIAEGLDLLADLGDELARALVDRPPAQLSEGDSIREGFDAELDELKDARDGGKT
                     YIAGLQARERERTGITSLKVGFNKVFGYYLEITNTHRDRIPADYERRQTLTGAERYVT
                     PELKEHEAKVLGAEERIATREAALIDTLRRRVGAEIARVQGTAASLATLDVWSALADV
                     AQRGAYVRPVVTDDFALRLEGSRHPVVERMMPREAFIPNDVQLDDAGRIILLTGPNMA
                     GKSTLLRQVGLAVLMAQVGSFVPAKSATIGIADRIFTRVGASDNLARGQSTFMVEMSE
                     TSAILHGATSRSLVLLDEIGRGTSTYDGVAIAWAVTECLHNRIGCKTIFATHYHELTQ
                     LTEELAHARNFNVAVKESGDDVVFLHRLEPGGADRSYGIHVGRLAGLPAPVVARAWEV
                     LKLLEAGHHVAHLPAPVPPDASQLGLFAPAERADPLLAELDRLDVNALSPLEALNRLA
                     EWQRRRKERP"
     gene            13622..14302
                     /locus_tag="VFU75_01510"
     CDS             13622..14302
                     /locus_tag="VFU75_01510"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEU5465531.1"
                     /translation="MKHFRHAGLLIALGAAACSHASGSGGAGSPPPAEEAPVAINPEV
                     PIAYPAALFDRGVDGEVKLRLFADSTGKLVPESTKILESSGYPAMDSAALSGAGKLKF
                     APGRRHGLPVATAFQQSVEFRHPSSAPVAPSDVGETKPLPPPVTLSNPASTGSRSTPV
                     TPAPKPKPRAQRPRPVVDTTRADSTARDTARAPVKTNSDTAKPKPDSIPPAPKPDSGP
                     KPVRPDSQ"
     gene            14299..15684
                     /locus_tag="VFU75_01515"
     CDS             14299..15684
                     /locus_tag="VFU75_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU5465532.1"
                     /translation="MTAHTKPYDDVLQTIGWTPMIRLRRLTQGIRTPIYAKAEFFNPG
                     GSVKDRIGVAMIEAAEKEGRLKPGGLVVEATSGNTGVGLALAAAVKGYRCIFTMPDKM
                     SQEKARLLRALGAEVIITPTAVAPDHPDNYIMKGRAIAAAHPNSIFADQHYNAVNPEA
                     HYRTTGPEIWTQTDGKVTHFVCSPGTGGTVSGVAKYLKEKNPKVRVIAGDPVGSIYRE
                     YGETHHKGEGAPYKVEGIGGDKIPTALDFAAVDEWITVSDKDAILLVRRMAKEEGLFC
                     GGSTGVNVFAALEVARKLDDPKACVVTVLSDTGERYLSKVYNDEWLRENQMLDSERVT
                     VASVLSAKDGKSPEVIQVAPQATVRQALNLMSTYNVSQLPVLDGADGVGAVSEQALMA
                     AALNDAAALDWPVREVMDPPFPVVDGDWPLARLTSLLSRETPAALVRRNGKISGIVTR
                     YDLLHQLAGIH"
     gene            15684..16706
                     /locus_tag="VFU75_01520"
     CDS             15684..16706
                     /locus_tag="VFU75_01520"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEU5465533.1"
                     /translation="MRVTVLTGGATAERTVAFAGATQVVAALRGRGHSVYVVDTATGL
                     LDEGDEKRLLKTDVGTTPPGDLTPLVQSERHFLGEGLATLPVVQGADVLFLVVHGGMG
                     EGGQLQALLDLIGVPYTGSGALGSALAVDKDLSKKLFRAAGVPVPEWFLAPVTPEDID
                     KTLGWPVIVKPSNGGSTIGLSLVRNAKGLDDAIRIARAYDDEVMIEQFIPGREMTVGV
                     LGDVPLPVGEIVPKHELFDYECKYTPGMSEETFPAQLPAPIAAKLQAAALLAHRALKL
                     RGYSRIDFRLTGAGDIFCLEANNLPGLTGTSLFPQGAKAAGIGYAEMCERICALARDT
                     RGSRKG"
     gene            16739..17551
                     /locus_tag="VFU75_01525"
     CDS             16739..17551
                     /locus_tag="VFU75_01525"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU5465534.1"
                     /translation="MTPWVRRLLVANLLVFLLQETVFLKAAQFLAFDPLHAFSRPWTV
                     LTYMFLHTGVLHLAFNLLALYIFGPPVEERLGSRGFIGFYLTSGLGGAVLSYALVLLT
                     HSAPILGASAAVYGVMLAFAWYWPDEPIFVFPLPEPILAKWLVIFFVLLSLTFALIPS
                     LQPGVAHVAHLGGFATAFLWLKLDAWRLGRAERRLRGAPERGVLVHPAALTARRGGET
                     QKTHPRGRSPERPNAEIDRVLDKISASGLASLTPAEKRFLAEMSKKMRDPHD"
     gene            17586..19568
                     /locus_tag="VFU75_01530"
     CDS             17586..19568
                     /locus_tag="VFU75_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HEU5465535.1"
                     /translation="MTFLPFLLFSLQDPLVAKAESLLARHDLRGARRIAETLVRHHPD
                     DPSAHMLLGRVWLEWPVIGRYQALDEFRAAQRLAPQDPEPWYWQTRVGLRLGDDEGEV
                     MVREAVLRILAIDPEYRDAWDLFSVVFHDAGIWRRADEALARHPDDPRAVEHRAGVAI
                     ARDDFALADSLAGAVLGRRRPYVPGYLLRAEADFGVGWDSAGYAWYDSAVAHADIDST
                     GAMWDEVWMIASPAEAARHDSTPPGERRRFFTWFWDKRDPNLVTPVNERIAEHFRRLV
                     YARRAFHILHPYSYYHRSATWRTLIDHDERDSLMRLLQAAPGLAPPGGMDSLFLATGM
                     GPARETVGDTAGSETIYLKAQLDARGILWIRHGRPDIWAGGMLEPGQGRIGSPLEFSS
                     WEYDTPDGILTIGLRDFRGGGVVLYPVTSRQFESAQSLLATDNTSIPAPLVADGWSAF
                     FKAGDFGLTDVYFRAAPETAAVALWEDGEPIARARGPGLMQLTVPPGDYDRGFDVDSA
                     GVLGRSRGQIKIPAYSQVQLGLSSLLLTPGDTVPDREAMLEGMPADLTYSGVRALAAY
                     TEVYGLARDKGGMSRYTARYTFAPERGLAARLLGNDKPVVFEFERSVIANAVTPERLV
                     IAPGLLAPGRYRVTLAVTDVLRNVKSQTVALSVTVR"
     gene            19630..20574
                     /locus_tag="VFU75_01535"
     CDS             19630..20574
                     /locus_tag="VFU75_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465536.1"
                     /translation="MLVNLIPEFLAAVAAPDPRGAYHEYLERHRPILRSYWSNYVLDP
                     DSKAADDVILSVLRADRADLLRLMQDIDVVQIAEDALRRTLETLEADCPVDLYLTVGV
                     GAANAGELVVGGRGIAFVCLEHFTGRANPQTSGLGLAPHLLPLWIAHEVAHAIRYTSP
                     SSRAALRRLVAEMGGNYDYWDTGSRAPLYEHLVNEGVAVAASQAVTPGFEPWQYFGYT
                     RRQYRRLRELDAFLRRASAPELDQSGLGLRLRYLSGGMSPTARLVMGKVLPERAGYYL
                     GARMVESYVGEVGIASAVRAAASEFRAAGERAMGAQTA"
     gene            20571..20780
                     /locus_tag="VFU75_01540"
     CDS             20571..20780
                     /locus_tag="VFU75_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465537.1"
                     /translation="MSAKRVTQERRSNRPLREVLDELINHTREIARRAKEMKPDELDY
                     AQQRLEWLADEVWRVATGQEAPPEA"
     gene            20787..21299
                     /locus_tag="VFU75_01545"
     CDS             20787..21299
                     /locus_tag="VFU75_01545"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEU5465538.1"
                     /translation="MSPDGTYRITVAKEDLNFAAAHFITFAGHRCETLHGHNYRAAVL
                     LDGKLDQESWYVVDFSAVKKIMRRLTGELDHKVLLPLENPRLQVTSKDGSVHVAYESK
                     PRYVFPEKDCVLLPIPNTTVEMLATLLAKKLAKELGEAGAKTITALELEVEENFGQSA
                     TYRMIVQEDL"
     gene            21296..21646
                     /locus_tag="VFU75_01550"
     CDS             21296..21646
                     /locus_tag="VFU75_01550"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HEU5465539.1"
                     /translation="MTKETALTDAQISDKLRSLPGWKYESGAIRRQYKTDGWPTTLML
                     VNAIGFYAESADHHPDLGVHWGSVEVALNTHSAGGITDKDFELAKLFEQCALWRPPAG
                     SAFRGTPKKFVSGS"
     gene            complement(21635..22273)
                     /locus_tag="VFU75_01555"
     CDS             complement(21635..22273)
                     /locus_tag="VFU75_01555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465540.1"
                     /translation="MKRVFAALSVIPFALVHAQGMAPVEQYRMDRAAEIALAKSAAPS
                     AISDAADVLVMGTAGYESALKGTNGFVCVVERGWAADYGSPDFWNPKVRSPFCYNALG
                     AQSVLADYLARTRQALSGMTEAKMEAAKPPLKLASGAMAYMLSKDGYLGDDVKGPWHP
                     HVMVFMPHMSAASLGANVEHSPTVVNDSPNSRVTVFVIPVVRWSDGSSDSAP"
     gene            complement(22270..22722)
                     /locus_tag="VFU75_01560"
     CDS             complement(22270..22722)
                     /locus_tag="VFU75_01560"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU5465541.1"
                     /translation="MASVTLVRRIKARPATVFDAFSTVEGLTSWWGPEDLPLLSAEAD
                     VRVGGTFQVRFPTSDGSVHECTGRFLEIRRPELITMSWQWTMGGEPTERGRSSRLELR
                     LRPIVDGTELTLIHAELGEDAGARSHGEGWNGALSKLIETYTTSEAGS"
     gene            complement(22722..23057)
                     /locus_tag="VFU75_01565"
     CDS             complement(22722..23057)
                     /locus_tag="VFU75_01565"
                     /inference="COORDINATES: protein motif:HMM:NF024248.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5465542.1"
                     /translation="MVKYHSSLDSTFAALSHPTRRAILTRLERVDGCTVSELSQFLVI
                     KLPALMKHLDVLANASLIRRFKESRTVTVRLSSMPLREAKNWLHRYQRFWTPRLDRLQ
                     AYAEAKEKR"
     gene            complement(23143..24456)
                     /gene="mnmE"
                     /locus_tag="VFU75_01570"
     CDS             complement(23143..24456)
                     /gene="mnmE"
                     /locus_tag="VFU75_01570"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF003661.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEU5465543.1"
                     /translation="MLSDPIVALATPPGRSAVALLRLSGRGAFAIAERCLSPWRDDPP
                     RTVFRARLATPAGVPVDDVLAALFPAPNSYTGEDLVEISTHGGLLAPAEAMAALAAAG
                     ARPAEPGEFTRRAVLNGKMDLLQAEATADLIDASAPAQRRRALAQLDQSLSKRLDALR
                     SEILELEGLTAYEIDFPEEDDGPVAPERVMKTWEGVRARVAALLGTAAEGERLREGAL
                     LVIAGPPNAGKSTLFNALLGSERAIVTEIPGTTRDAIEAPAVIAGFPFRLVDTAGLRD
                     SQDRIEKMGIEVSKRYLEAADVVLYCDERGQEAKALGTRAPVIAVRTKADLGPLASSP
                     LSVSAVTGSGMKELKARLAEVAFSKLIALGDVEPVVTRARHRQALETALKELDLFKES
                     RESGVEAVAAATHLRAATGALDSLIGAVTPDHVLDRVFSSFCVGK"
     gene            complement(24467..26170)
                     /gene="yidC"
                     /locus_tag="VFU75_01575"
     CDS             complement(24467..26170)
                     /gene="yidC"
                     /locus_tag="VFU75_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR03593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0051205 - protein insertion into membrane
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEU5465544.1"
                     /translation="MDRRVFIALVCFALVIIVPSLIWPPKPTPKRVDGQTDTLSQAAA
                     VVDSSRLDSSRAGAPPNQLAVKPSSRLSAPADTVWVTTPLLKVGVSTRGGTIVSARLS
                     QYKSYAAGDSGRQVELVPAGRPILGRRIAIGSDTLDLTDLSFAPSTRVMSVAGDPATL
                     TLTATRGPSTITIAYTFHPDDYRVGVRGRVTGLGPSGAQMLVSLGDGLRNSEADSSGS
                     FRDYALVTKSSKTEKHAFSSVTEGERSVFDGPFEWAGIKSKYFLVGALATEPGEAQFA
                     GVVAVGGPRPDSAKGFFGGKSGIATEMSVSLTLPVPPAGDFQYSVFLGPLEHRRLAAI
                     GHDMDDANPYGWAIFRPIIRWVSILVTNILLAGHESLGVAYGWLLIAFGVLVRLVLWP
                     LNQKAMASQMRMQAVAPLLKEVQTKYKNDPERQQKETLKIYKEHNVNPLGGCLPMLIP
                     YPMLLALYFVFQYMIELRGSSFLWLPDLSRFDPYFILPIVMGLSMFALSKIGQRGMPA
                     NPQTATMLYMMPAMMTFIFLRLSSGLNLYYLVSNLFSLPQQWMLSQKRLKDQGKRAGA
                     T"
     gene            complement(26195..26389)
                     /gene="yidD"
                     /locus_tag="VFU75_01580"
     CDS             complement(26195..26389)
                     /gene="yidD"
                     /locus_tag="VFU75_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013936843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEU5465545.1"
                     /translation="MGLIRVYQLTLSHLVFTQCRFTPTCSRYTYEAVERYGALKGSWL
                     GIRRVARCHPFHPGGYDPVP"
     gene            complement(26419..26730)
                     /gene="rnpA"
                     /locus_tag="VFU75_01585"
     CDS             complement(26419..26730)
                     /gene="rnpA"
                     /locus_tag="VFU75_01585"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEU5465546.1"
                     /translation="MGRGPELHACWNTGKRVRLPHLELAWLPRTLPARSRSGIIVPLY
                     QRTAVQRNQLRRRIREILRRETLRLLPPVDLVVRARREAYGASFAVLRDELNQAAAKI
                     K"
     gene            complement(26760..26921)
                     /gene="rpmH"
                     /locus_tag="VFU75_01590"
     CDS             complement(26760..26921)
                     /gene="rpmH"
                     /locus_tag="VFU75_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEU5465547.1"
                     /translation="MGPTYRPRNRRRINKHGFRTRMKDKWGRAVLSRRRKKGRKRLTI
                     RIASKHRSR"
     gene            27049..28434
                     /gene="dnaA"
                     /locus_tag="VFU75_01595"
     CDS             27049..28434
                     /gene="dnaA"
                     /locus_tag="VFU75_01595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEU5465548.1"
                     /translation="MEQSAKEAWKRLLEEARRELPDSIVRTWLEPAEPIALDEGRLIV
                     GAPDQFAVEWNESKHSGLLARAAERVLGQPTTVVFKVQDERQHRPQMDFFVAAKDGQT
                     AVTERPASTQPLNERYTFESFVVGKSNELAAAAAHAVVEAPGLTYNPFFIYGATGLGK
                     THLMQAIAHAVIARNPGTRLLYVGAEQFINDVIESIQARTMPEFRRRYRNEIDLLLVD
                     DVHFLEGKEMTQDEFFHTFNALFEGRKQIVLTSDRPPKEIPGLEDRLVSRFEWGMVAD
                     IGQPDLEHRIAILRKKAHQDHLELTIPDDVLRFIAEHVRSSVRELEGCIIKLLLFASL
                     KNREVSIDLAREALSDKIRQGDDAGTFNNQAAPSIDRVQEVVARRWGVTPEGLRSKAR
                     TKTLTIPRQVAMYLARDMLGMQLVEIGQAFGGRDHSTVIHSVDKVERQMMRDRIFKER
                     IESARTELSAL"
     gene            28642..29748
                     /gene="dnaN"
                     /locus_tag="VFU75_01600"
     CDS             28642..29748
                     /gene="dnaN"
                     /locus_tag="VFU75_01600"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEU5465549.1"
                     /translation="MKFTITRDQLQEGLTAVAAAIPAKTTLPVLSNVLVEATKQGVRL
                     SGTDLDIAVSTMVSADVDAEGAATLPAKKLVDIARELPPAAIRFTGASEQRVSIECGK
                     SRFKLLGLPREEFPSFPAVKFDGASKAAAGVVQKLINHVAFAASTEESRPILNGVLWE
                     LRPDRMRMVATNGHRLAKMDVPSTGSAAADLIVPPKALDQIRRLFAADAEIEIAKSDN
                     HIGFRSGGKLVFSRLIEGPYPPYENVIPRENDKSATVDRNAMAGALRRMSVVASDQTH
                     RIRLALSGGTCRFSVQTPDLGEAQDELAVTYEGEPLEIGFNATYLLEILKFMPTDEVR
                     FTFKAPERAATVEPVRWNDPASYMALVMPLRLVD"
     gene            complement(29651..30616)
                     /locus_tag="VFU75_01605"
     CDS             complement(29651..30616)
                     /locus_tag="VFU75_01605"
                     /inference="COORDINATES: protein motif:HMM:NF013106.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HEU5465550.1"
                     /translation="MRRRLGRWAAGLAVLALIWLLGVWPPPVWWRFHTPRTTAMMREE
                     STTPATSGESFRGSRLPTVHPVPLSSIAPVMQRMVIIGEDSRFKTHHGIDFAEIADAL
                     QLTPHSKLWPTVQAVWRRRDRIRGASTITQQLAKNLYLSPSRNPLRKLKEAVTAVRLE
                     LALPKDRIMELYLNVVEWGPGVWGVDQASRDYFGVPPSGLSDEQAAELAATLPHPRSS
                     NPLFHPAITLARRDLILARFRGVDVYIPPDTTAADSLLMPLIPQAELDSIPVVIPVDS
                     TKSDTMKTDSAADSSRRASAASRAPYSSPGRSSVPARPSRPAPEP"
     gene            complement(30623..31357)
                     /locus_tag="VFU75_01610"
     CDS             complement(30623..31357)
                     /locus_tag="VFU75_01610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465551.1"
                     /translation="MNRSDTYILPSEAQDARAIWVTPSGLAVRQEASVYFDLTVANPG
                     SLGQLRQLNVGFNARGLGFAYQRDQFSGGTSAGTYKLAFGTGQGPMAVGLGTTWYRGG
                     TRGTGFDVGMTYRASGALALAGVIANIGQPNVRGLVQRVRYTASANLQVTPGLGLGAL
                     TSANPSGFRAFGADLRAAFDPGFPLGFVARMDTDQKFRRTQFAFGFSIGGLSQMGLVA
                     TTPGNASSINDVTLYGVSSRLPTRRH"
     gene            complement(31423..32295)
                     /locus_tag="VFU75_01615"
     CDS             complement(31423..32295)
                     /locus_tag="VFU75_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465552.1"
                     /translation="MRISVSALAVVTAAAVACGGTIAPTSTAGGPPVPASATPAIPQS
                     APLRYSPGVTKYVISQRHHVDQQLPTGDQVQEIGLRTYVTAIIIGPADARGYSVSITI
                     DSILADSATVLPPTVDLQSARGLRYDGRLSPWGQLIDPRPSDAAIAKNLAQLFGGFRT
                     FFPRLPSAGVAPGVKWSDSTEATDTTGTAVVVDRSVSHYATGEWESLAGVRQLGIQVS
                     GDFTVSGSGAAGGATFALSGTGTHAGKLQFSAAGRFLGGSTVDSAGIVITFDPQGMAI
                     PRHQVSRTTIVVLP"
     gene            complement(32295..32459)
                     /locus_tag="VFU75_01620"
     CDS             complement(32295..32459)
                     /locus_tag="VFU75_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465553.1"
                     /translation="MRVFRTTSEEWSKLIAWRADMRRRGWSLLRVSSEGQEMVAVFGR
                     TKTDRSGATT"
     gene            complement(32506..32878)
                     /gene="rnpB"
                     /locus_tag="VFU75_01625"
     ncRNA           complement(32506..32878)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VFU75_01625"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(32943..34826)
                     /locus_tag="VFU75_01630"
     CDS             complement(32943..34826)
                     /locus_tag="VFU75_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465554.1"
                     /translation="MTKRGLPLLALLTLGACRPTASRLLLLDYTLADPVDLQTTAAPW
                     HDAGYTVEYRRFFPHLTRDDMRRYHGVVLLGGLRPDEPSDALTSGDAVILTEWIRAGG
                     VVVLGYPESALDRWTMNRWLKSLGAGVVIADSAPPDTQRIALPIRRTLPSADLVPIEL
                     EDARTLRIRLPAQALARVTPATRGAPQPVVVAATRVAAGLVVVTSRAALAADDAGTTK
                     DFLETVARWTRRPAEWANVPAATQPVPLSLEGGPDSVRARLTLASPPGGSRPVTLPLR
                     PDLPVKDSVTPPDWIGRAGLRVLWAEPLGYRTGYAERKVALDSLTALLDVGAFNVFAG
                     PANVEAVAESTHYAPWERTVSQNVWKVMGDRLEQTSARWVPALEPDSFRLPPDTAGVA
                     AGRCLLDPGYWNAAFVPGMISLARLAAQHQDLIPAVAVDLEREPSSTQPAATICEADY
                     DAGVDGMVKDSAISAAQGAGLKKVAPQDRYDVLLNEGLVDDLFEALERVTTTRASDLR
                     ARIQRIRPGLLLAVRAAAPPQDWFGRGLLRGWSAPGTPVILLTRDPSSRQTNARYRGR
                     GINVLSAFELVPRDVDQSGWAALRPLAFRENSGFWLDARRSGVSAGQDSTWRLVRRLA
                     REK"
     gene            34891..35751
                     /locus_tag="VFU75_01635"
     CDS             34891..35751
                     /locus_tag="VFU75_01635"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5465555.1"
                     /translation="MTVCTVLPVPERSRVDAVGDGCFTTVHVETFREVLQAARRRKVD
                     ALLISVHRCAGDEIPAVARFVREFPAIPAVALVSRHDGVATETLLRLGATGVRSAVDC
                     TEPGAWRRLRDVFGHPITPAAARILARLVPALGDAPEETRVFFEAVVRLAPVIVTVRG
                     LARHLRVTPSTFVSRFHRATLPSPKRYLSAMRLLHAAYLFQNPGLSVSDVAYRLDFSS
                     PQSFGRHLKAILGVTAGEFRSRFPFEVALARYVDLLITPYREALRAFYPLNAGFWDQG
                     SDVPRVSRAG"
     gene            complement(35758..37464)
                     /locus_tag="VFU75_01640"
     CDS             complement(35758..37464)
                     /locus_tag="VFU75_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEU5465556.1"
                     /translation="MTIPSLHPEKRSLANWLRGRSDDSAGLEQRLKDLDTFARSLSRR
                     WVMVSGVFFVGILGWLSGTIRTSPGTIVLAAGGGAILNALVGLVNERGWYRWWLIYVL
                     TLLDVLLAALPVVWFGHGGLIAGFFIAVLPYTFDQGRTVGDFLVLVGSLAYLASTMLH
                     GSLYEGSADIPPAAYLETVVFVAVAMALKRIPATLITRIRATRSIMGSAEQGQLGVRA
                     AAAESDELGFLEKSLNRMLDEIGATISTVQHEADEVAAFAEELAAAAEQIHATSEAVT
                     QTAKGLADDLVGQRTVAEGAQAESAKAAAQAESLKTRAERMQSDAGQLASTAARGRDR
                     VERASQTLRSVREEVRATAATVGGLSAMSDRIGGFAQTIARIARQTHLLALNAAIEAA
                     RAGEHGEGFGVVAQEVRGLAGEAGKSAREVAELVSEIRTGITAAARAMETGESQVRDI
                     GVVAAEADGALRELHEGVQVIGGLVDATAEVSRTQAERLAQLAQSLAQVSTVSGVSAE
                     HATSAASATQAQITSMGDLTATSQQLAQLAERLRASIARFAVLKPEQSTAEHRVVRAA
                     AD"
     gene            complement(37461..38012)
                     /gene="pyrE"
                     /locus_tag="VFU75_01645"
     CDS             complement(37461..38012)
                     /gene="pyrE"
                     /locus_tag="VFU75_01645"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEU5465557.1"
                     /translation="MTDLDRLRELLLSRSVTRGDFTLSSGRKSTFYIDARRTTMSAEG
                     LALIGSLGVERLVARGWAPRAVGGLTLGADPVAYAIAAASRNRSPAPVVDAFTIRKQA
                     KTHGTGRRIEGCFEAGQSVVVVEDVITTGGSAQEAIAALSAEGAKILGVFAVVDREEG
                     GREVLERAGHAVETLLTAKGLGL"
     gene            complement(38051..38959)
                     /locus_tag="VFU75_01650"
     CDS             complement(38051..38959)
                     /locus_tag="VFU75_01650"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5465558.1"
                     /translation="MPTENLAGQTIAGYRLLERVGEGGTAEVYRAEHAERGPSAFKVL
                     RPRLRGDPTAVKRFLREAGYGARVSHPGVVRTFDYGEADGLYYLALEWAPGDSLAAFL
                     HRNGPLAPPEAVALVRQLADALAAAHHAGIIHRDLKPENIMYDPATKRARLLDFGIAR
                     DTEVPPEERLTRTGFFVGTLQYVAPEALSGELVDGRADIYSLATIAYYLLTGRHPYSG
                     RSPRELFQQLLTQPPEPLNQAVPGLKFSAGLEAAIMRGLARDPGARQPTVEALAAELE
                     QGLAADPTPPPSKGLFGALKSIVGRK"
     gene            39001..40359
                     /locus_tag="VFU75_01655"
     CDS             39001..40359
                     /locus_tag="VFU75_01655"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HEU5465559.1"
                     /translation="MIRRVAVPVVLGMSLVSCGQWSRVGSPDNPKPAVQVSRLFDAAA
                     QYRSMGLLAAGDPLPWVGAVRWLAGSTPDSTLALVSLSFANHSLRFRRDGNDFVASYR
                     VELSFHGANGAPRTITRDETVRVRSFQETLRADESVIFQQFVSVPPGPCTIAIVVRDR
                     DAVGASRQEVVDTIPSFAGPTLSTPIPVYEGAGRSRRDTVPALVSNPRATLPYGNDTL
                     FFYVEAYNLPAGNPIAAAVLSPSGDTVWADTLRLDPREGVARTIFRIPPAKLPVGQER
                     FYAAAVGTGKGVQAPFLVSFSSQWVVTNFDEMLSLLRFFPRQDLVTKLRSAPDSLRPQ
                     AWRDFWNASDPVAMTPENEALNAYLSRVQVASQRFTESADPGWLTDRGEVFITLGDPD
                     EVYDLESDYSRTGLHGIRWIYTGLRLDLLFLDQTGFGRFRLTPGSRADFDRAATRLRH
                     EN"
     gene            40359..42578
                     /locus_tag="VFU75_01660"
     CDS             40359..42578
                     /locus_tag="VFU75_01660"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEU5465560.1"
                     /translation="MRRAVWALSLCWPLALPAQKPAEVARAESLEIAGRPWHAAEAFV
                     AGAGTAAAPASAQVLIADARAELTARRYARVVALLDKRSWLADSGDGVGYAILAEAED
                     RLGRSADAARDYLRARSLATGSRAALLAVRAAVVFDRAGADDSARFYYVAARAAGLPS
                     IDEWLRLREAGVSRDTTIVDSLLDGLTFPASRRAPLYRARALLAAGDSGLAVDALAAA
                     PGGALQAAEVALARRDSAWARKLLYHLFATAPQSDDAAAGEALAQGPLPPFSSAERTS
                     LAQVLRTHGDAPAALVQVRRAVASGDSSAATLILLGDLLVSNRKDRDALKAVSAAITM
                     ATDSATRALATYRRARVLVRMGDPGAAQALSDYARAYPGDSSAVTALYLAGDRLAEQG
                     DSTGAEAMFRELVSAHALDAASVQARLRLGSWEYRRRHFDRAAEWFRGEVAASPGVHG
                     AARYWLAKIAAAQGDTADARAQWTALAHDDSVGYYGLRARTAAGLPPLTVAEGVDSTV
                     PAVTTGLARLDTLRLAGLDSEADAQVRALVANPPDSVAQLLAWTSGLASRGWGSLSVR
                     LAWLAAPRAPGNPRVLRAIYPWPNRGAVVAEANEFHVDPFLLAALIRQESVFDREALS
                     RVGARGLTQLMPSTAAYTARGLDLPFTPEWLTVPDLNLHLGAAHLGTLLARYHGRVDA
                     AVASYDAGTTPVDRWLARAPGADPDLFIEDVAYVETRGYVRSVLRNWEVYRALYGAN"
     gene            42599..45436
                     /gene="polA"
                     /locus_tag="VFU75_01665"
     CDS             42599..45436
                     /gene="polA"
                     /locus_tag="VFU75_01665"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEU5465561.1"
                     /translation="MPPRSTDALFLIDGYALIYRAFFAMIARPLTTRHGENTSAAWGV
                     TNFLLRLRERHQPGYLGWINDRGVSFRERLYPAYKATRQKLTAELQQDFDRSVERIAQ
                     LLEAFRVPLVTVEGFEADDVIGTLAAKATDQGVLSVIVSGDKDFYQLIGPKVALLNPG
                     RSGPAGVEEQWVDESNASERLGVPPRQVIDYLALVGDSSDNVPGVKGIGEKTAVELLA
                     KYPDLETIISHAAEIPGKRAREALLAHTEEARLSRDLVTIRRDVPVTLDLDALRVKPF
                     DGPRLTELFTELEFRSLIAKVTGPDAAVPIRSSAPAAEPAAQPAPAPAAAPQAVPAVA
                     AVPARVLDDVAGLAAMIAECRRAPMIALDTEASAIDPLRASLVGLSIAVAPGQSWYLP
                     FTHRPPDGELAGGVPPKNLPAIKSPELAPLRELLADPAVKKAGHNIKYDWLVLRRAGV
                     ELAGVAYDSMLASFVLDPGRRSHALDALALERLNLPMRTYADVAGRGRAERPFADVPV
                     PDAARYCCDDSEMVLRLAEAFNGELDDHHLRPLLDTIEMPLIRVLADMEWAGIRVDRE
                     LLANLSRQFAIDLGRLEKEIHAAAGTDFNIASTPQLRTVLFEKLQLPVLKKTKTGAST
                     DYEVLEQLAGMGHEVPRLLIEYRELSKLKSTYVDSLPLNINPDTGRIHTSFNQTGAAT
                     GRLSSSDPNLQNIPIRTPRGEEIRRAFIASPGSVLLTADYSQIELRLLAHLSGDPLLV
                     EAFERGGDIHRQTAAVIFGVPDDQVTSEMRSRAKTINFGTIYGQGPFALARMLSITQD
                     EAKRFIAEYFQRFAGVRTWLDRTVAEARQRGYTETIFKRRRYIPELKDKNFNMRAFGE
                     RTATNSPLQGSAADLIKVAMIRIAAGLAEGKLGTRMLLQVHDELVFEVPVDEREAASV
                     LVKREMEGAARLRVPLVVSIGTGPNWVEAKD"
     gene            45481..45861
                     /locus_tag="VFU75_01670"
     CDS             45481..45861
                     /locus_tag="VFU75_01670"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU5465562.1"
                     /translation="MRPPRGFTLIELLIVIVIIGILATMAILNYTSSKQKTYEASMKA
                     DLRNLATAEEAYFYDSSSYTNSFAMMSNYTPSAGNAIVVNEATRVGWSATASSTGTGR
                     KCYLFFGSATPVGNATKEGVAACS"
     gene            45900..46151
                     /locus_tag="VFU75_01675"
     CDS             45900..46151
                     /locus_tag="VFU75_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465563.1"
                     /translation="MIYAMDGGVWLHRHVFHDEPMIHLVSTRREPLLLVGDRLGLDPA
                     WLQYKPLKDPRTGVRVEAWHWDLWGESKGRLEALLETGQ"
     gene            complement(46145..48415)
                     /locus_tag="VFU75_01680"
     CDS             complement(46145..48415)
                     /locus_tag="VFU75_01680"
                     /inference="COORDINATES: protein motif:HMM:NF015155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HEU5465564.1"
                     /translation="MIALPAKMLIRRRWWVLFAWALVAALLAPSAARVQDALAVRGGS
                     DESTESFRAGEALKAALPNPIAEYVAVVVHGPVPVNHPRFVRVLDSLSAALSRKGYIK
                     QVVSAQSLGESTFVSKDRHTTFLIAAMQTEQTDNIKNNFVPDIRSTLAGVVAGIPGAG
                     AFDVKLTGDPALDYDIRTISAEDTQHGEHVAVPFSLAVLVVAFGALVAATLPIIVGVL
                     AITIALGLITIVAHFQTMSVFALTITSMVGLGVGIDYSLLIVTRFREELNRGLAAADA
                     AARTIQTAGSAVITSGATVVVGFAALLVTPLIETRSVGIGGLLVVAIAIGIATTFLPA
                     LLAILGRGIDRPKWLARPLARFHAPTGWERWARWLGHRPWRAIALGGGIIGLLMIPLS
                     QMKIGLPARNWFPSESESGVGLQELVNMGVSGIIQPTRVVITLPPGESALSARRLLGL
                     KALGDRIRKDPRVKQVRGVASLQPHLSSLALTMFYANPDSVRRHHTTFFNAYLSADSR
                     TTLVDVIPEDSVTLTGLMQMATNVRQTARKGIRGLDGATILVGGFAATSVDLQNDLLS
                     RFPTIVALILGVTAVMLFIAFKSLLVPLKAVLLNCLSVGGAFGITVLVFQRGVLGWLF
                     RLDGPTEAIFVVVPVLVFAVVFGLSMDYEVFLLTRVKEVFDKTGRNDHATMEGLSSTA
                     STITFAAAIMVLVFGVFAFSRVLAVQFMGFGLAVAVLLDATLIRMVLVPAIMHIAGRW
                     NWWPGVRAPKDPPPGH"
     gene            48526..49353
                     /locus_tag="VFU75_01685"
     CDS             48526..49353
                     /locus_tag="VFU75_01685"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF012630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031012 - extracellular matrix [Evidence
                     IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEU5465565.1"
                     /translation="MALQLLALAVVAVVVFRFAARHRTPRAPLPGHRAPVATLPPRHD
                     SLGAQVAATPDTAGRDTTPVPPINPMDTLTMNEPQGYYDQLARAQTRRQIRASAGTTY
                     LGEIVSESSDSALHRWDHRYEDPVRIYLTTDTVENFQPAFVDAVRSALTRWDQVNLPV
                     RFTFVDDSTRAEVTFRWRRQFDIDRTGQTDLTWDRDGHVLSAVVTIATFDPNNHPMGV
                     EDVRVVALHEIGHMLGLNHSSDSTDVMYGHTLARDLSLRDERTAQLLYELSPGSVRN"
     gene            49350..50444
                     /gene="amrS"
                     /locus_tag="VFU75_01690"
     CDS             49350..50444
                     /gene="amrS"
                     /locus_tag="VFU75_01690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HEU5465566.1"
                     /translation="MSRNPLPLLATSSPAGPSLADTLDTRTMPASLVTPEVDGAVRCV
                     ACGHRCLVRPGRRGICKVRFNRDGVLRAPAGYVAALQCDPTEKKPFFHCLPGSDTLTF
                     GMLGCDFHCGYCQNWLTSQALRDPSAGVPPTEVSAEALVGLALRHGARVMGSSYNEPL
                     ITAEWAVEVFRLARARGLKTAFISNGNATPEALDFLRPWTDCYKVDLKAMDPARYREL
                     GGVLEHVTDTIAAAHARGFWVEVVTLIVPGFNDDRPQLKRAAELIASVSPDIPWHVTG
                     FHPDYRMTDPAATPTETLIEACEVGVAAGLRFVYAGNRPGAVGSWENTYCPDCGDLLV
                     ERRGYQILAQRVGSDGRCPSCARAIPGIWA"
     gene            50588..>51425
                     /locus_tag="VFU75_01695"
     CDS             50588..>51425
                     /locus_tag="VFU75_01695"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU5465567.1"
                     /translation="MSGIKQGAYPGAVLVVGRRDRILYEKGFGHLTWSPRSPVDRPDS
                     TIFDLASLTKAIATTTAAMILYDRGKLKLDVPVSTYLPEFNGAGTAAITVRMLLTHTS
                     GLRGDLPVPELRALPDGTALIRTVLAETPRTKPGTRVVYSDLNFVLVGEIIRRLSGEP
                     LDQFVTTEVIGPLELTETRYRPSTRLASRIAPTGVWHGHPVAGVVNDPTAAKAGGVSG
                     NAGLFSTGENLARFAQFLLRGGTRPDGKPLVSPETVKLFTTRASQADVGPDHRALGWQ
                     AVP"
BASE COUNT         8588 a        17433 c        17051 g         8353 t
ORIGIN      
        1 ccacgtaacc ctttcggccc accctgtgtc ccatcccagt gaagcccgga agcacccgtc
       61 acccggagcc ccgtggacga ccgtgaattg atcgccagag tccgcgccgg agacccggcg
      121 gcggagcggg cgctgtacga cgcgcatgtc gaccgcgtct accggctgtc gtatcgcatg
      181 gccggcgacg acgacctcgc gcgcgacttc acccaggaga cgttcatccg cgccttcgag
      241 cgactggaca cgttccgcgg tgagtcggcc ttctccacct ggctccacag catcgcgacc
      301 acggtggccc tgaacggtct ccggaagctg aagcggttcc ggaagcgcga gacggacatc
      361 gaggaagcgg acggcgtcac ggcgagccgg gcgaacgcgg aaccggatct gaagaaacgg
      421 ctggcgcagg cgatcgatgc gctgccggag ggataccgga cggtgttcct gctgcacgac
      481 gtggaagggt acacgcacga agagatcggc gccgcgctcg ccatcgagtc cggcacctcg
      541 aaggcccagt tatcacgagc ccgggcgaag ctcagaatcg ctctggccga ttttgcggga
      601 gaatggatcg gatgaacgag aaccgggaag accgtttcga gcaggagctc aagcgcatgg
      661 cacaggacta tcaccgtccg cccgagaccc cgcgcgatga aatgtgggca cggatcacgg
      721 cggagcgcgc ccggcggcgc gagtccatga aggtggttcc gctccgccgt tggatggcct
      781 ggagcctcgc cgccgccgcg gtcctggtgc tcggcgtcgc catcggccgc tggagcatga
      841 acgtgaagga gaagaacccc ggaccgcgga acgaggagat ggcgtaccgc gctgctgcgg
      901 cgccctatct cacccggacc gaagcgctgc tcaccagttt ccgcgccgag tcgcggaatg
      961 ggaaagtgga cgccgacttc gccgtccagg cgcaggacct gctcgtcacg acgcgcctga
     1021 tgctgaactc ccctgccgcc agggaccccc gcctcgcgag cctcctgcag gatctcgaga
     1081 cgatgctggt gcaaatcgcc cagctcccgg ccggcaacga ccggaatgat gtcgatctga
     1141 tcaatcaagg actcgatcaa cgaagcgtga tgctccggct gcggaccatc ggtcccacgc
     1201 cgggcacttc tcacagagga gcactctaat gatttccctg catctcctgc tcacggccgc
     1261 cactttcatc cagacgccgg tcggggcgcc ggtgcctccg gtcccgctga cggccgtcgc
     1321 ggcggtcgcg ccggccctgg ctcaggcgag ggccgcgctc gacatcgcgc gagcacaggt
     1381 tgctccgctg agccgggtct acccgagcat cgccatggcc tacgccgggc acgcccgagc
     1441 ctatgccgcg ctggcgcgcg atcaagtgcg cgccgcgcgg tgggccacca gggctcccgt
     1501 gggcgagtac acggatgatc ccggtgactc gctctacaat gccgcgcggt cgctgttgaa
     1561 ccgcgcccgg tacaatgaag ccgcgagcgc gttcgccgac ctggcgaagc ggtatcccaa
     1621 gtcgcggtac gcgcccgatt cctactattg gcaggcgttc tcgctctaca agaccggtga
     1681 cgcctccaag ctcaagcaag cccggtccgt gctcgagtat cagcaacagc atttccccga
     1741 cgccgcgacc cacggggatg ccgacgccct ctatgcccag atccagggtg cgctggcccg
     1801 tcagggtgac ggccccgcgg gtgagtgggt gcagaaacat gcgagccagg cggacgccga
     1861 gcgtccgacc acgagcggga gctgcgcgtc gagcgacgat gacgacgacg atccccggat
     1921 cgcggcgctc aacgccctgc tccagatgga cgccgaccgc gccgtgccgg tgctgaagaa
     1981 ggttctggag cgccgcgacc cctgttccgt gtccctgcgg cgcaaggcga tcttcatcat
     2041 ctcgcagcag cacaccgcgg agaccgaaga catcctcctt ggcgccgccc ggaacgatcc
     2101 ggacgaagac gtgcgcgaac aggcggtgtt ctggctctcg caggtgggga ccgccaaggc
     2161 cacggcggcc ctggactcga tccttctgaa ctcgacgaac accgagctcc aggacaaggc
     2221 gatcttcgcg ctgtcccaga tcgattcccc cgaagcctcc aaggcgatcc gggattacgc
     2281 cgagcggccc ggcgcgccgg tcgaggcgcg gggcaaggcc atcttctggc tctcgcagca
     2341 gcatgacggt gccaacgcgg cctatctccg cgcgctgtac gggcgcctga ccgacgacga
     2401 cctcaaggac aagacgatct tcgccatttc gcagctcggc ggtgccgaca acacgaattg
     2461 gctcgtcgac ctcgcggcca acaagcagga gtcggtcgag atgcggaaga aggccctgtt
     2521 ctgggccgga cagtcgaacg aagtgtccct cgatcggctc accggtctgt acgaccggat
     2581 ggacgaccgc gacatgaagg agcagatgat cttcgtctac tcccagcggc acgaaggggc
     2641 ggcactcgac aagctgatcg acatcgcgaa gcacgacacc gatcccgagc tccgcaagaa
     2701 ggccatcttc tggatcggtc agtcccacga tcccaaagcc gcccagtacc tgcaggaggt
     2761 catcagtcaa tgatccgggc cctcgcggcc ctgggcgcgg cgctcctggt cgggagcgcc
     2821 gctgccgctc agggcatcag ccaccgggtg agtgccgccc cggacggcaa agttcgcatg
     2881 acgttcgcgt cccgccccgg cgtctgcggg aacggctcca acgacatctc gatggattgt
     2941 gaggacggga actgcgggaa tcacaacacg tattcgcggg acgaccacga cgtggacgtg
     3001 acgacctgcc cctgcgaaga aggacccgtt cgcctcgtct tgcgggtgtc gggcggcaag
     3061 gtgacgcgca tccgcgccta cgtcgggggg gaatggaaac ccgcggaggc cggtactacc
     3121 gacctgggag tcgtggggga caaggatgcg gtcgcctact tgctcgatct ggcgcgcacg
     3181 tcttcgagcc acattggaga ggaggcgatc ttcccagcca cgctcgccga ttcggtgaac
     3241 gtctggcccg acctgttcaa gatcgcgcga agtgacgagg tcccggcgca aaccaggaag
     3301 tcggcggtct tctggatcgg ccaggccgcc ggggagacgg tcaccaaggg tctcgactcg
     3361 ctcgcccagg gcagcgacgt ggaccgtgag gttcgggagt cggcggtatt cgcgctctcg
     3421 cagcagcgga acgacgtcgg cgtgccggcc ctgatctcca tcgccaggac caacaaggat
     3481 ccggacatcc gcaagaaggc catcttctgg ctgggacagt ccaacgatcc tcgggccctc
     3541 gcgctcttcg aggagcttct caccaggaag taaccggcac tccggcgcgg ggggtgaacc
     3601 gttcgtcccg tcgggaccgc ggttcatccc cactcgccct tccgctcaca cgttgctgac
     3661 ttccgcgtgc aaccaagttg ccgggttgga cgtccgacct cgtatgctcg ctctcctcct
     3721 gctgcttcag ggcccccaga cccagaagcc cacgctgccg tcttcgcccg tcgactccag
     3781 tgcccttcgg cactccaatg gtctcgtccc ccccatggcc tttggcgttc gcgtgcagca
     3841 ggcgcccgcc atggacggca agctcgacga tccgctttgg gcgacggcca ccgcggtcac
     3901 cgacttccgg caggtcgatc cccatgaggg gcagcccgcg tcggagcgca ccgaggtgcg
     3961 ggtgctctac accgacgacg ccctctacat cggcatccgg gcgtgggacg actcggcgtc
     4021 gaagatcgcg caccacctgg gccgtcggga caccttcaca cagagcgacg acttccgcgt
     4081 cctgatcgac agctaccacg accaccgcac cgcctttcgc ttcgacatca cgcccaccgg
     4141 cgtaaagggc gacatccagt tcggcgatga cggcaacttc gccgatcaat cctgggaccc
     4201 ggtgtggacg gcggtcacgt cgatcgattc actggggtgg accgcggagg agaagatccc
     4261 attctcccag ctgcgcttct cccgcgccac cgaccagatc tggggcatcc gcttcgttcg
     4321 caccattctc cggaagaacg agttcgatct gtttcccttc gtcggaaaga cggaaaacgg
     4381 attcgtgtcg cgcttcgcgc atttgggcgg tctcaagaac attccggcgc cccgccgtct
     4441 cgagctgctt ccctacacgt cggggtccgg aaccttccag cctcagggtc ccgtcggcaa
     4501 tccgttctcg aagacgagcg cctattcggg gaatgccggc ctcgatttga aatacggcgt
     4561 cagctcgttc ttgaccctcg acgccaccat caatccggat ttcggacagg tcgagctcga
     4621 cccggcgttc gtgaacctca cggcgttcga gcagttcctg cccgagcatc ggcccttctt
     4681 cgtggaaggc gcggacatct tcaacttcgg cggcgggtcc ggtgggctcg cgaacttcgg
     4741 ctccaccccc cggttcttct attcgcggcg catcgggcgc cggcctcagg gtgacacgta
     4801 ttcgtccggc gatttcgtgg actatcccgc caatacctcc atcctcggtg ccgccaagct
     4861 gaccgggaaa acgccttcgg ggtggtcggt ggggttcctg gatgcggtca cctccgagga
     4921 gaaggcgtcg gtgctggata cgacgagtca cttgacggcg cgcgatctgg tcgagccgct
     4981 gtcgaacttc atggtgggcc gggtgcggag gacgctcaac ggcggccgat cgagctacgg
     5041 gggaatgatc acggccgtga atcgggacct cgcggttccc tcgctccgcg gcctgaattc
     5101 gggggcgtac accttcggcg tcgatacgaa gcagctctgg tcccggaaca cgtacgcgct
     5161 cgccgccgac gtcggcggct cgtacatcac cggtgatacg acggcgatcc aggacgctca
     5221 gttctcgtcg gggcgctact accagcggcc cgacgcgcgc gcattccact atgatccgac
     5281 ccggacgtcc ctggccggct tcaacggcaa cgtctacctg gacaagttgg cgggcacgtg
     5341 gctctggggg tccgcgatct ccacttcttc cccgggattc gaggtgaacg atctcggatt
     5401 ccagccgcgg gtggaccgga tctccggcta cgcgtacctc gcccggcatc agacgacgcc
     5461 gggaccgctg ttccgggaat cgtgggcggt gctgacggtg ggaccttcgt ggaactacga
     5521 cgggaatccc atccagaagg ccgtgacggc cagctacttc ggcaatctca acaacttctg
     5581 gttcctccag gtgcagggga actacggggc gcaggtggtc gatgaccgcc tgacccgcgg
     5641 tgggcccctc gccctggcgc cggcgttctg gaacgggttc gcgatgatcc agaccaatca
     5701 gagcaaggcg ttcacggcga tggtcatggg gtcgtacaac aaggacaacg ccgggagctg
     5761 gcagttcaac gtcagcccgc aactggggta tcgcgcctcc agcgccctga acttctcgat
     5821 cacaccgaat ttcaccgtgg gtcccacggc ggcccagtac gtgcaggcag tgaccgactc
     5881 caccgccggc agcaccttcg ggtcccggta cgtcttcgcc cagctgttcc agcatgagtt
     5941 cgacgtaacc ttgcgggtca gcgccaccgt gtccccaaac ctgtcactcg aagtattcgc
     6001 ccagccgttc agcttcacgg gatcctacgg cgggttcaag gagctggccc aacccaagac
     6061 gttcgcgttc aaccggtacg gggaaaacgg gacgtcgatc aatttcaacc ccacggattc
     6121 gctctaccac gtcttcccca acgctgcgca gccggcggac tctttcacgt tcgcgaaccc
     6181 cgatttccgc acccggtccg ttcgcatcaa cgcggtactg cggtgggagt accggcccgg
     6241 atcgacgctg ttcatagtct ggacccagag ccgcgggggg atgttctccg atcccacgtt
     6301 cgacatcggc cgggacttcg atcatcagtt gttccaggat ccgccgacca acgtgctgct
     6361 cgtcaagctg aattactgga tgagcctgta aacgggagcc gggagccgag agtcgtcgca
     6421 gtccaggata tggaagctcc gccatccatg aggagcttcc cttcgccccg actcagacgg
     6481 ctcccgactc ccgactcccg tatatttgtt ccccatgccc tcggcccgtc atgccttcaa
     6541 gatttctctg tccctcgcac tccttctcgc cgccggctgg gccgcggtgg tgatcgacgt
     6601 cttgatcacg ggctcccgtg agggcgccgc accggcggac gccatcgccg tgctcggcgc
     6661 ggcgcagtac aacgggaagc cgtcaccggt gttcaaggct cggctcgaga aggcggcctg
     6721 gttgtaccgc tcgggaaaag cgccggtgat cctggtgacg ggtgggatcg ggaaccggga
     6781 cacggtgagc gaggcggagg tgggccggcg ctatctcacc cacatcggcc tccccggaga
     6841 ctccgtgatc gccctccccg ccgccgcgac gacggtctcg tcgatcggcc agcttggcgc
     6901 ctggttcaag gaccggccga gcaaacgcgt ggacctggtg agtgacggct ttcatctcct
     6961 gcgcctgcgc atcctcgcca atcgccaagg cctcgtgccc ttcacgtctc cggccaaggc
     7021 gtcccccatc agcgcgagtc tccgcaccaa cgccgcctac gtcgccgccg agggatggaa
     7081 ggtcccggtg gcttggctct tttatcgcta ggatgaggag ttcctgatgc aggtcggtac
     7141 gagacgcgtg cacaccggca aggtcatcga tctcgacgtg gacacggtga agtatcccga
     7201 cggatcctcc ggggagttgg agatgatccg tcaccccggc gcttcggcgg tggtcccggt
     7261 gctgtccgat cccgatgggc ctgatccgac gatcctgttg atcaagcagt accgctacgc
     7321 caccggcggc atgctctggg agattccggc gggaaggctc gggaagggcg aggcaccggt
     7381 ggactgcgcc cgccgtgagc tcctggagga gacgggcatg cacgccggga agatcgagcc
     7441 cctgatcccg atctggacca cgccgggctt cacggacgag cgcattcatc ttttctgggc
     7501 gcgggacctg accccgggcg aaatggcgcg tgaggccgac gagttcatcg agctcgcccc
     7561 ccggcccctg tcgcaagtgc tgacggctat tcgagatggg gagatctgcg acgccaagac
     7621 ggtgacggcc atcctgctgg tagccggttt tgtgttcggg ttgtagtctt tctgatgtcc
     7681 acccgggagg acgtgcggct gaagcaggtc tgtaacgtct tccttaatca aatgttaatg
     7741 aagtcaaatg ggtggtacac ttcctgcctc aaacttgaga ggaggattaa gccaagcgtt
     7801 ccctactttt ccgtctgaaa cctgggagta cacgatgcgg gcaatcctca agcggaagac
     7861 gacaccggtc gccacggacc gcgccatcga gcgggagaag ctgcacgagc tcgatgacgg
     7921 gcacgtcgtg tcccggcacc tcgccggcga cccgcaggcg ttcggtaccc tggtcgaccg
     7981 gtatcaggtc cggctgctga atttcgtgaa ccggaccatc ggcgatcgcg agcgggcgga
     8041 agacctggtg caggaagtgt tcatccgggt gttccggcac atccaccgct tcgaccagac
     8101 caagaagttc tcgacgtgga tttacacgat cgcttccaac ctggcgaaga acgagctccg
     8161 gaaccggagc cggaatccgc tggtgctgtt ccagaccatc aagaagaact gggacgccga
     8221 tcatcggccg ttgcagttcg aagacacgac cgcgcgtccg gacgatctgt atcgcaagcg
     8281 ctacctgaag gacgccgtgg actcgtgcgt gaaggagctg cccgagcatc atcgggcggt
     8341 attcgtgctg cgggagctgg aagggcggag ctatcaggaa atcgccgaaa tcacgggctg
     8401 caacctgggc accgtgaaga gccgcctcaa ccgggcccgg aacacctttg cgcatctgat
     8461 cggcccgcaa ctcgattgag cgacgaaccg cggaaccacc cgccggcacg ggaggtatag
     8521 tgcccaggat ggaatgcgcc gagtttctcg atcgctatac ggattaccgc gacggagtgg
     8581 tgaccacccc gcgcgaccgc cggcggttcg agcgtcatct cgcccattgc gagacgtgcc
     8641 gctcgtacga cacagcggtt cgtcgcgggg tgcttgcact tcagggggtc gagaccctga
     8701 ccccctcgga acgcttccgc gcccgcctgg atgcccggct ccgccgggag gcgttgacga
     8761 tggcgggccc gcggatctct ccgcgcatcg gggccgccgc cgcgctcctc gccgccgtcg
     8821 gcatcgcgct cgtggccctg cagctcgccc accgtcccgc acccctcgtg gcccacgcgc
     8881 tcccgccggt gcccttcccg aagcccgtag cccaggccgg cgtaccgttc gtcacgttcc
     8941 aggacccgcg cgccatgacc gccgccggca accccatgcc ctacggctcg gcgctcgtcg
     9001 agccggtcag cgcccaccac taggctttca tccgcttcac gcgggcggta ttatccgccc
     9061 gatggcgacg ttgagcttca acgcgctcct cggcggcggc aagccggggt cgctcgatcc
     9121 ggtctactat ctccacggcg aggaagagat cctgaaggag gaggcggtgc gcctgctgct
     9181 cgaccgcgcc gtcgatccat ccttgaggga tttcaacgtc gacgcacggt ccgcgcccga
     9241 gctggatcca gccgctctgc gcaccctggt cgatacgccc ccgatgatgg ccgaccgccg
     9301 ggtcgtgatc ctccggtccg tcgagggctt tcgggccaag tcgaagaccc gggacgagct
     9361 ggtccgctat ctcgcgcacc ccaatcccag caccaccctg attctggttc agggcggcga
     9421 cgaggcgccc gacgcggggc tcactgcgca cgcgaccacc gtcgccgccc tccggctccc
     9481 acccgaccgc gtcacgcgct ggatcacaca ccgggccggg cagatcggca tcacgatcga
     9541 caccgccgcc gctgagctgc tgggccgggc gacggcgcat gacctggccg cggtttcgca
     9601 ggagctcgac aagctggccg ccatcgccgg cacgcggacc gtcaccgagg cggacgtcac
     9661 cgccgccgtc ggtgtccggc atggcgagac gctcgacgat ctgatcacgg cgacactcga
     9721 gcgacgggtg ggtgacgccg caagaacgct cgggcccgtg ctggcccagt ccgggatgag
     9781 cggagtcaag ctcgtcagca cgctgggcac cggtctcgtg ggtctcgccg ccgcccgtgc
     9841 cgagctcgac cgggggacgg cgccgggacg gctggagtac acgatcctcc agcggctgat
     9901 cgcgtcgcgg cccgcagggc tgggggccgg gtacaaggac gtagccgcgc gctgggcgaa
     9961 gtgggtgcgc ggatggaccg cccccgagct gtcccgcgcc atccgccgcg cgctcgccgc
    10021 ggatcgcgcg ctcaaggcaa cgcaactgac cgacgaatac ggcatcgtgc tcgacctgat
    10081 tctcgggttg gcgcgtgcct cgagggaggc agcgtgaact acagacggct agacggttgg
    10141 acgggtagag gggtcatggc catggtgctt gcactagccg tctacccgtc tagccgtcta
    10201 gcgagtcaat ccgatcctcg tttgaaatcc gccgtcgatc tcgctcagtc gggccaggtc
    10261 gactccgccc gctccatggt gcgcaagctg ctggcgacca tgtcgccgca cgactccgcg
    10321 tatcctcagg cactctacac gcagggcatg ctggccgcgg acgcgccgac cgtcacctcg
    10381 aatctccagc gcgtggtagt cgagtatggg tggtccccct gggccgccaa cgcactcatg
    10441 cggctggccc agctcgccta cactcagggt gacccggcgt ccgccattca atcggtccag
    10501 cgtctgcgcc gcgattatcc cgactcgccg gtgaaaccgc gcgcggattt ctggggcgcg
    10561 aaagccgact tcgacctgcg gaacgaaacc gaagggtgcg agctcatccg ggaagccctc
    10621 gccggcgccg gcaccgacgt cgagttcaag aatcaggtgt cgttctacgc ggcgcgctgc
    10681 ggcgcgactc ccgcgccgac cccgaccgcc agcgcgccgc cagccgcggc aactcccgcc
    10741 gcccccacga gtccgccgag ggacacgtca ccggccacgt cgggcaagac cacgtacgcc
    10801 gtgcaggtgt tggccgtgaa gagcgccgcc gacgtcgacg ccctgctcac acgtctcaag
    10861 gtgatggggt acgaagggca cgtcgtaccg cgcgactcca ccggcctcat caaggtgcgc
    10921 gtgggccgct acgcgactcg acccgaggcg ctcagggcgc agtcgcagct caaggcccgc
    10981 ctcggcggcc agccgttcgt ggtgaccgag ccttgagctc acgagtggcg tcggctcccc
    11041 ctccggctga tacgccgctc atgcagcaat accgcgagat caaatcgcgg catcccaata
    11101 ccattttgtt tttccggatg ggtgacttct acgagatgtt cgaggacgac gcgaagctgg
    11161 gtgcgcgcgt cctggggctc acgctcactt cgcgcaacaa cggcggcgcc tcggaggtcc
    11221 ccctcgccgg cgtacccgtg aaggccgcca ccgagtacct ccggaagctg gtctccgccg
    11281 gccaccgtgt cgccgtatgc gaacaggtcg aggatccgaa gctcgccaag ggtctggtgc
    11341 gtcgcgaagt catcgaaacc atcaccccgg gatcggtcct cgccgaggac tggatcgcga
    11401 ccggcaagaa caacttcctc gccgcgctcc tgccccagga gtccgtcgtc gggcttgcgg
    11461 cgctcgatct caccaccggc gaattcgtct gggagaccct gctccccgag gatgtcgcgg
    11521 cgggactggc ccgctatgag ccgtccgagc tggtggttcc cgcgggtggc gcggggcccc
    11581 gcgagctcac cggcacgcga accgagcgag aggcctggga attcgatccc gatctcgccc
    11641 gggaggatct gcagcgagtg ttcggtctcg cttccctgga tggcctgggt gtcgagagca
    11701 ccgaccgcgc cgcactggga gccgccggtg ccctgctccg ctatgcccgg gagctgaagc
    11761 ccggaggact gccccatctc gcccggccga gactcgtcag gcggggggcg gtgctgccgc
    11821 tcgacgaaat gacgcggcgg aatctcgagc tcatcgagcc cctccgtccc ggcgcgacag
    11881 gaacgaccct tctcgaagtc ctcgaccgca cgctgacgcc catgggagcg cggctcctgc
    11941 gccgctggct cttggcgccg ctcgtcgacg caggcgccgt gaatcagcgc ctcgacgcgg
    12001 tccaggtgct cgtgatggat ccgcgcggac gcgaccggct gcgcgaggcg ctcgacggcg
    12061 tccgtgacct ggagcgcctc gccggccgcg cggcggtggg ccgcgccacc ccgcgcgagc
    12121 tcggcgccct gcgcgattcc atcagaaagc tccccgacgt ccgggccggc ctcgacgggc
    12181 tggaggctcg agaccactcg gcccttctcg aggaaatcgc ggaaggcctc gatctgctcg
    12241 ccgatctggg cgatgagctg gcccgcgccc tcgtggaccg gccgcccgct cagctctcgg
    12301 aaggggactc catccgcgag ggctttgacg cggagctgga cgagctgaag gacgcgcggg
    12361 atggcgggaa gacctatatc gcgggtcttc aggcccgtga gcgggagcgg accggcatca
    12421 cgtccctgaa ggtcgggttc aacaaggtct tcggatacta cctcgaaatc acgaacaccc
    12481 accgggaccg gatccctgcc gactacgagc gccgccagac tctgaccggc gcggagcgct
    12541 acgtcacccc cgagctgaaa gagcatgaag cgaaggtcct gggcgccgaa gaacggatcg
    12601 cgacgcggga ggcggccctg atcgacaccc tgcgtcgccg cgtgggtgcg gagattgcgc
    12661 gcgtgcaggg gacggcggcg agtctcgcga ccctcgacgt gtggtccgca ctcgccgacg
    12721 tcgcgcagcg aggagcgtac gtcaggcccg tggtcactga cgacttcgcc cttcggctcg
    12781 aaggatcccg ccacccggtg gtggagcgca tgatgccccg ggaagctttc atccccaacg
    12841 acgtccagtt ggacgatgcc ggacggatca tcctgctcac cggaccgaac atggccggca
    12901 agtcgaccct gctccggcag gtcgggctcg ccgtcctgat ggcgcaggtg gggtcattcg
    12961 tcccggcgaa gagcgccacg atcggcatcg cggaccgcat cttcacccgg gtgggtgcgt
    13021 ccgacaacct ggctcgcggt cagtcgacct tcatggtcga gatgagcgaa accagcgcga
    13081 ttctccatgg cgccacgtcc aggagcctcg tcctgctgga tgagatcggc cgcggtacgt
    13141 cgacgtacga cggcgtggcc atcgcgtggg ccgtcaccga gtgtctgcac aaccggatcg
    13201 gctgcaagac catcttcgcc acgcactatc acgagctgac ccagctcacc gaagagctgg
    13261 cgcacgctcg gaacttcaac gtggcggtga aggaatcggg cgacgacgtc gtcttcctgc
    13321 accggctgga gccgggcggc gccgaccgat cctacggcat tcacgtcggc cggctggcgg
    13381 ggctccccgc gccggtcgtc gcgcgcgcct gggaggtcct caagctcctg gaagcgggtc
    13441 accacgtcgc gcatctgccg gccccagtac ctccggacgc ttcgcagctc ggcctcttcg
    13501 cgccggccga gcgtgccgat cccctgctgg cggagctcga ccggctcgac gtcaacgcgt
    13561 tgtccccatt ggaagccctg aaccgtctgg cggagtggca acgccgccgg aaggagcgcc
    13621 catgaaacac ttccgtcacg ccggcctcct gatcgcgttg ggtgcggcgg cgtgcagtca
    13681 tgcgagcggc tcggggggcg ccggctcacc gccacctgcc gaagaagcac cggtggccat
    13741 caaccccgaa gtcccgatcg cctatccggc cgccctcttc gaccggggcg tggatggtga
    13801 agtgaagctg cggctgttcg cggactccac cgggaagctc gtcccggaat ccaccaagat
    13861 cctcgagtcg tccggctacc cggccatgga ctcggccgcg ttgtcgggcg cggggaagct
    13921 caagttcgcg ccgggccggc gacatggact ccccgtggcg acggcgttcc agcagtcggt
    13981 ggaattccgg cacccatcga gcgcacccgt cgcgccgtcg gacgtcggcg agaccaaacc
    14041 cctgcccccg ccggtcacgc tttcgaatcc cgccagcacc gggagtcgct cgacaccggt
    14101 gacgccggcc ccgaagccga agccgcgggc gcagcggccc cgccccgtgg tcgacacgac
    14161 cagggccgac tcgacggctc gtgacacggc aagggccccg gtgaagacga attccgacac
    14221 ggccaagccg aagcccgatt cgattccgcc ggccccgaag cccgactccg gacccaaacc
    14281 cgtccgcccg gacagtcaat gaccgcgcac accaagccct atgacgacgt cctgcagacc
    14341 attggctgga cgccgatgat ccggctccgc cggttgaccc agggcattcg gacgcccatc
    14401 tacgccaagg ccgagttctt caatccgggt gggtcggtga aggaccggat cggcgtcgcg
    14461 atgatcgagg ccgcggaaaa ggagggacgg ctcaaaccgg gtggtctcgt cgtcgaggca
    14521 acgtcgggaa acaccggcgt gggactcgcc ctcgccgcgg cggtgaaggg ctaccgttgc
    14581 atcttcacca tgcccgacaa gatgtcccag gaaaaggccc ggctgctccg cgccctgggg
    14641 gccgaagtga tcatcacgcc gacggccgtg gctccggacc atccggacaa ctacatcatg
    14701 aaggggcgcg ccatcgcggc ggcgcacccc aactccatct tcgccgacca gcattacaac
    14761 gccgtcaatc cggaagccca ctaccgaacg acgggacctg agatctggac tcagaccgac
    14821 ggaaaggtta cgcacttcgt gtgctcgccg gggacgggtg ggacggtgag tggcgtcgcc
    14881 aagtacctga aggagaagaa cccgaaggtg cgggtgatcg ccggtgatcc cgtggggtcc
    14941 atctaccgtg agtacggcga gacccaccac aagggggaag gcgcgccgta caaggtcgag
    15001 ggcatcggcg gggacaagat cccgacggca ctcgacttcg ccgccgtcga cgagtggatc
    15061 accgtgtccg acaaggacgc catcctcctg gtgcggcgca tggcgaaaga agagggtctg
    15121 ttctgcgggg gctcgaccgg cgtcaacgtc ttcgcggcgc tcgaggtagc gcggaagctt
    15181 gacgacccca aggcgtgcgt cgtcacggtg ctctctgaca ccggcgagcg gtacctctcc
    15241 aaggtctaca acgacgagtg gctgcgtgag aaccagatgc tcgattccga gcgcgtcacg
    15301 gtcgcgagcg tgctgtccgc gaaggacggc aagagtcccg aggtgatcca ggtggccccg
    15361 caagccacgg tgcgccaggc cctgaatctc atgagcacgt ataacgtctc ccagcttccg
    15421 gtactggacg gcgccgatgg cgtcggcgcg gtctccgaac aggcgttgat ggccgccgca
    15481 ttgaatgacg ccgcggcgct cgactggccg gtgcgtgaag tgatggatcc gccgttcccc
    15541 gtggtggatg gggactggcc cctcgcgcgt ctcacgtcgc tcttgtcccg ggaaacgccc
    15601 gcggcgctgg tgcgccggaa cggcaagatc tcggggatcg tgacgcgata cgacctcctg
    15661 caccagctgg ccggcatcca ctgatgcgcg tcaccgttct caccggtggc gcgaccgccg
    15721 agcgcaccgt cgcgttcgcg ggggcgacgc aggtggtcgc ggccctgcgt ggccgtggac
    15781 actcggtgta cgtcgtggac acggcgaccg ggctgctcga cgagggagac gaaaaacggc
    15841 tcctcaagac cgacgtcggg accacgcccc ctggcgatct cactcccctg gttcagtcag
    15901 agcggcactt cctgggtgag ggcctggcga ccctgccggt ggtccagggc gcggacgtgc
    15961 tgttcctcgt ggtgcatggc gggatgggcg aggggggcca gctccaggcc ctcctcgacc
    16021 tcatcggtgt gccgtatacc ggctcaggtg ccctgggcag cgccctcgcg gtcgacaagg
    16081 atctctcgaa gaagctcttc cgcgccgccg gcgttcccgt ccccgagtgg ttcctcgccc
    16141 ccgtgacgcc tgaggacatc gacaagaccc ttggctggcc ggtgatcgtg aagccctcga
    16201 atggcgggtc gaccatcggg ctttccctgg tgcgaaacgc caaggggctc gacgacgcga
    16261 tccgcatcgc ccgcgcctac gacgacgaag tgatgatcga gcagttcatc ccgggacggg
    16321 aaatgaccgt cggggtgctc ggcgacgtgc ccctgccggt cggggagatc gttcccaagc
    16381 acgagctgtt cgactacgag tgcaagtaca ctcccggcat gtccgaggag accttccccg
    16441 ctcagcttcc ggcgccgatc gccgccaagc tccaggcggc cgccctgctc gcgcatcgcg
    16501 ccctcaagct ccgtgggtat tctcgaatcg acttccgcct gaccggcgca ggcgacatct
    16561 tttgcctgga agccaacaat ttgccgggac tgacggggac gagtcttttc ccccaaggtg
    16621 ccaaggccgc cggaatcggc tacgccgaga tgtgcgagcg aatctgcgcc ctggcgcgcg
    16681 acacccgggg gtcccggaag gggtagtaga gggcatgggt ccgcaacgga tgttcgggat
    16741 gacaccgtgg gtccggcgac tcctcgtcgc caacctcctg gtgttcctgc ttcaggaaac
    16801 ggtattcctc aaggccgccc agttcctcgc gttcgacccg ctgcacgcgt tctcccgccc
    16861 gtggaccgtc ctgacgtaca tgttcctgca cacgggcgtc ctccacctcg ccttcaacct
    16921 gctcgcgctc tacatcttcg gtcctcccgt cgaggaacgt ctcggctcga gagggttcat
    16981 cggcttctac ctcaccagcg gcctcggtgg cgccgtgctg tcgtacgccc tcgtcctctt
    17041 gacccactcc gcgccgatcc tcggcgcgtc ggccgcggtg tacggcgtga tgttggcgtt
    17101 cgcgtggtat tggcccgacg agccgatctt cgtgttcccg ctccccgagc cgatcctcgc
    17161 gaagtggctg gtcattttct tcgtgctgct gtcattgacc ttcgcgctga ttcccagtct
    17221 ccagcccggc gtagcgcacg tggcgcacct gggcggcttc gccaccgcgt ttctgtggct
    17281 gaagctcgat gcctggcgcc tggggcgggc ggagcgtcgt ctgcgcgggg ccccggagcg
    17341 tggcgtgctg gtgcatccgg cggcgctcac cgcacgccga gggggcgaga cgcagaagac
    17401 gcatccccgg gggcgctcgc ccgagcggcc gaacgcggag atcgaccggg tgttggacaa
    17461 gatttcggca agtggtctcg cctcgctcac gccggcggag aagcgctttc tggccgagat
    17521 gagcaagaag atgcgcgacc cccacgactg agtgcgtcgc cgggcggcac gctcgtctct
    17581 ctcttgtgac cttccttccc tttctactct tcagcctgca ggaccccctc gtcgccaagg
    17641 ccgagtcgct tctggcgcgc cacgacctga ggggagcacg gagaatcgcc gagaccttgg
    17701 tccgccatca tccggacgac ccctccgctc acatgctgct ggggagggtc tggctcgaat
    17761 ggcccgtcat tggccggtac caggcgctcg acgaattccg ggcggcccag cggctcgcac
    17821 cgcaggaccc ggagccctgg tactggcaga cccgcgtcgg actccggctc ggagacgatg
    17881 agggagaggt catggtgcgg gaggccgtgc tccgcatcct ggcgatcgat cccgagtacc
    17941 gtgacgcctg ggacctgttt tcggtggtct tccacgacgc cggtatctgg cgccgcgcgg
    18001 acgaagccct ggcgcgccat ccggacgacc cgcgggcggt cgaacaccgc gccggtgtcg
    18061 ccatcgcgcg ggacgatttc gcgctcgcgg actcgcttgc gggtgcggtg ctcgggcggc
    18121 ggcggcccta cgtccccggc tacctgctcc gagccgaagc ggattttgga gtgggctggg
    18181 acagcgccgg gtatgcctgg tacgactccg cggtggctca cgccgacatc gattcgaccg
    18241 gtgcgatgtg ggatgaagtc tggatgatcg cgagccccgc ggaggccgct cgacacgatt
    18301 cgacaccgcc gggagagcgg cggcgcttct tcacttggtt ctgggacaag cgcgacccga
    18361 acctcgtgac ccctgtcaac gagcgcatcg cggagcactt ccgccggctg gtgtacgcgc
    18421 ggcgggcgtt tcacatcctg cacccctaca gctactacca ccggtcggcg acctggcgca
    18481 cgctgatcga ccatgacgag cgcgattcgc tgatgcggct cctccaggcc gcgccggggc
    18541 tcgcgccgcc gggcgggatg gacagccttt tcctcgcgac ggggatgggt ccggcgcgcg
    18601 agacggtcgg ggacacagcc gggagcgaaa ccatctatct caaggcccag ctcgacgccc
    18661 ggggcatcct ttggatccgt cacggtcggc cggacatctg ggcgggtggc atgctggagc
    18721 ccgggcaggg ccgcatcggc tccccgttgg agttctcttc ctgggaatac gacacccccg
    18781 acggaattct caccatcggc ttgcgggact tccggggcgg aggcgtcgtc ctgtatcccg
    18841 tgacgagccg tcagtttgag agtgcgcaat cactgctcgc cacggacaac acgtcgatcc
    18901 ccgcgccgtt ggtagcggac ggatggtcgg cgttcttcaa ggccggggac ttcggcctga
    18961 cggatgtgta cttccgcgcc gcgcctgaga ccgccgccgt cgcgctctgg gaggacggag
    19021 agcccatcgc ccgggctcga ggacccggac tcatgcagct gacggtcccc ccgggcgact
    19081 acgatcgcgg cttcgacgtc gactcggcgg gcgtgctcgg ccggagccgg ggtcagatca
    19141 agatcccggc gtactcacag gttcaactcg gtctctcgag tctcctgttg acgccgggag
    19201 acacggtgcc tgaccgcgaa gcgatgctcg aggggatgcc cgccgatctg acgtattcgg
    19261 gcgtgcgcgc gctcgccgcg tacaccgagg tgtacggcct ggcgcgtgac aagggcggca
    19321 tgagccggta cacggcgcgg tacacgttcg caccggagcg aggactcgcc gccagactcc
    19381 tcgggaacga caagccggtg gtgttcgagt tcgaacggtc ggtcatcgcg aacgccgtca
    19441 cgccggagcg cctggtgatc gcgccgggtt tgctcgcgcc gggacgctat cgcgtgacgc
    19501 tggcggtgac cgacgtcttg cggaatgtca agtcgcagac cgtcgccctc tcggtcaccg
    19561 tccggtaggt gtctcttgtc tccctgaaag gggcttccca aattggggcg gctcagcgcg
    19621 cgcccctaca tgctcgtaaa cctgatacct gaatttctcg ctgccgtcgc tgcgcccgat
    19681 ccccggggcg cgtaccacga gtacctcgaa cgtcatcgtc ccattcttcg cagctattgg
    19741 tccaactacg tcctggatcc cgactccaag gcggctgacg acgtcatcct cagcgtcctg
    19801 cgggcggatc gggcggatct gctgcggctc atgcaggaca tcgacgtggt ccagatcgcc
    19861 gaggatgcgc tcaggcgcac gctggagacg ctcgaagccg attgccccgt cgacctgtat
    19921 ctgacggtgg gagtgggcgc cgcgaacgct ggcgagctgg tggtgggggg ccggggcatc
    19981 gcgtttgtgt gcctcgagca cttcacgggc cgggcgaacc cccagacctc ggggctgggc
    20041 ctggcgcctc acctcctgcc cctctggatc gctcacgagg tcgcccacgc catccgttac
    20101 acgtctccct ccagccgggc cgccctgagg cgcctggtcg cggaaatggg tggcaactac
    20161 gactattggg acaccgggag ccgggccccc ctctacgagc acctcgtgaa cgagggggtg
    20221 gccgtagccg cctcgcaggc cgtcacgccg gggttcgagc cctggcagta cttcggctac
    20281 accaggcgcc agtaccggcg gctccgcgag ctggatgcat ttcttcgccg cgcctcggcc
    20341 ccggagctgg atcagtcggg gctgggcctc aggctccggt atctctccgg aggcatgagt
    20401 ccgacggccc ggctggtgat gggcaaggtc ctgccggagc gtgcgggcta ctacctgggc
    20461 gcccggatgg tggagtcgta cgttggcgaa gtggggattg cgtcggcggt gcgggcggca
    20521 gcctcggaat tccgggcagc cggtgagcgg gccatggggg cacaaacggc gtgagcgcca
    20581 agcgggtgac ccaggagcgt cgctcgaaca ggcctctgcg cgaggtcttg gacgagctga
    20641 tcaaccacac ccgggagatt gcccggcgcg ccaaggaaat gaagccggac gagctcgact
    20701 acgcccagca gcgtctggaa tggctcgcgg acgaggtatg gcgggtagcc acgggacagg
    20761 aggcaccgcc cgaggcataa ttaagcatga gcccagacgg tacctaccgc atcacggtcg
    20821 ccaaggagga tctcaacttc gcggccgccc acttcatcac cttcgccgga catcgctgcg
    20881 agaccctgca cgggcacaac taccgcgcgg cggtcctcct cgatgggaag ctcgaccagg
    20941 aaagctggta cgtcgtcgac ttctcggcgg tgaagaagat catgcgccgc ctgaccggcg
    21001 agctcgatca caaggtcttg ctgcctctcg agaatcctcg gctccaggtg acgtcgaagg
    21061 atggctcggt gcacgtggcc tacgagagca agccgcgcta cgtattcccc gagaaggact
    21121 gtgttctgct gccgattccc aacaccacgg tcgagatgct cgccacgttg ctcgcgaaga
    21181 agctggccaa ggagttgggc gaagcgggtg ccaagaccat cacagccttg gagctcgagg
    21241 ttgaggagaa cttcggacag tcggcgacat atcggatgat tgtccaggag gacttgtgac
    21301 gaaagagact gcgctcaccg atgctcaaat cagcgacaag ctcaggtcgc tgcccggctg
    21361 gaaatacgaa tcaggcgcca ttcgacgcca gtacaagacc gatggctggc ccacgacctt
    21421 gatgctggtg aacgcgatcg gcttctacgc cgaatcggcc gatcatcatc cggacctggg
    21481 cgtgcattgg gggagtgtgg aggtggcgtt gaacacccac tccgcgggtg gaattaccga
    21541 caaggacttc gagctggcga aactcttcga gcagtgcgcc ctctggcgtc ccccggcggg
    21601 cagtgcgttc cgggggacgc cgaagaagtt cgtctcaggg agctgagtcg gagcttccgt
    21661 cggaccaccg caccaccggg atgacgaaaa ccgtcacgcg ggagttgggg gagtcgttga
    21721 ccaccgtcgg cgaatgctcg acgttcgcgc ccagggacgc tgccgacatg tgcggcatga
    21781 agaccatgac gtgggggtgc cagggtccct tcacgtcgtc gccgaggtat ccgtctttcg
    21841 acagcatgta ggccatcgct ccacttgcca gcttcagcgg cggcttcgcc gcctccatct
    21901 tcgcttcagt cataccggaa agcgcctgcc tggtccgggc gaggtaatca gccaggaccg
    21961 actgggcacc cagcgcgttg tagcagaagg gcgagcggac cttggggttc cagaagtccg
    22021 gacttccgta gtcggcggcc catccccgct ccaccacaca cacgaatccg ttcgtcccct
    22081 tcaaagctga ctcgtaccct gccgtcccca tgaccaggac gtcggcggcg tccgagatcg
    22141 ctgatggcgc ggccgacttc gccagcgcga tctcagcggc ccggtccatc cggtactgtt
    22201 ccacgggagc catgccttga gcatgaacca gggcgaacgg gatcaccgac agtgctgcga
    22261 acacgcgttt catgagcctg cctccgatgt agtgtaggtc tcgatcagct tgctcaacgc
    22321 accattccag ccttcgccgt ggcttcgcgc cccggcatct tccccaagtt ccgcgtgaat
    22381 cagcgtcagc tcggtgccgt cgacgatcgg tcggagacgc agctcgagcc gggacgaacg
    22441 gccccgctcc gtcggctccc cacccatggt ccactgccag ctcatggtga tcagctcggg
    22501 cctcctgatc tccaggaacc ggccggtgca ctcgtgaacc gatccgtcag aggtcggaaa
    22561 ccggacctgg aaggtcccgc ccacgcgcac gtccgcctcg gccgaaagga gcggcaggtc
    22621 ttcgggtccc caccaggacg tcaggccttc aacggttgag aacgcatcga acacggtcgc
    22681 ggggcgggcc ttgatccggc gcaccagggt gacactcgcc atcagcgctt ttccttcgcc
    22741 tcagcgtagg cctggagtcg gtcgaggcgc ggagtccaga agcgttgata ccggtggagc
    22801 cagttcttcg cttctcgaag cggcatcgag gaaagccgca ccgtgacggt ccggctttcc
    22861 ttgaatcgcc ggatcaggct ggcattcgcc aggacgtcga ggtgtttcat cagggccggc
    22921 agcttgatga ccaggaactg cgacaactca ctgaccgtgc agccatccac ccgctccagg
    22981 cgggtgagga tcgcacggcg cgtggggtgg ctgagcgccg cgaaggtgct gtccagtgag
    23041 gaatgatact ttaccatgtg gtgaagtatt atcggaacaa gaacccggtc aaggtgggac
    23101 cgggccgacc gcgccgagtc gcgttaaagg ggcgccgagt agctacttcc ccacgcagaa
    23161 gctcgagaag acccggtcca gaacgtggtc cggggtgact gctccaatga gcgagtcgag
    23221 cgctcccgtt gccgctcgca agtgggtggc cgccgcgacc gcttccactc cgctttctct
    23281 cgattccttg aagaggtcga gctctttgag ggcagtctcg agggcctggc ggtggcgggc
    23341 ccgcgtgacc acgggctcca cgtcccccag cgcgatgagc ttgctgaatg cgacctccgc
    23401 gagccgcgcc ttcaattcct tcatcccgga cccggtcacg gcagagacac taagaggcga
    23461 agaggccaag gggccgagat ctgccttggt ccggaccgcg atgactgggg ctctcgtacc
    23521 tagcgccttc gcctcttggc ctctttcatc acagtacagc accacatccg ctgcctcgag
    23581 gtacctcttg ctcacctcga ttcccatctt ctcgatgcgg tcctgagagt ctcgcagtcc
    23641 tgcagtatcg accagcctga acgggaatcc ggcgatgacg gcgggtgcct cgatggcgtc
    23701 ccgggtagtg ccgggaatct ccgtgacaat ggcgcgttcc gagcccagca gggcgttgaa
    23761 aagcgtggac ttgccggcgt tcggaggccc cgcaatcacc agcagtgctc cctcgcgaag
    23821 tcgctcccct tccgccgccg tccccaagag cgcggccacc cgcgcccgca caccctccca
    23881 ggttttcatg acccgctccg gcgcgaccgg gccgtcatcc tcttccggaa agtcgatctc
    23941 gtacgcggtg agtccctcga gctccagaat ctccgaacgc agagcgtcga gccgcttgga
    24001 cagactctga tcgagctgag cgagcgcccg tcgcctctgt gccggcgcgc tcgcatcgat
    24061 gagatccgcg gtcgcttcgg cctggagcag atccatcttg ccattcagta ccgcccgcct
    24121 ggtgaactcc cccggctcgg ccggccgggc tccggctgcc gcgagagcag ccatcgcctc
    24181 ggcaggcgcc agcaagcctc catgggtcga gatctcgacc agatcttcgc cggtgtagga
    24241 attgggggct ggaaacaggg cggcgagcac atcgtctact ggaacaccgg ccggggtcgc
    24301 caaccgcgcg cgaaagaccg tccggggagg atcatcacgc cagggagata ggcacctctc
    24361 ggcaatcgca aatgcgcccc ggcccgagag cctgaggaga gccaccgccg atcgtcccgg
    24421 cggtgtggcc agcgcgacga tcggatccga aagcatcagc cgatcgtcag gtcgccccgg
    24481 cgcgtttgcc ctggtccttc agccgttttt gcgaaagcat ccactgctgc ggcaggctga
    24541 agaggttcga caccaggtaa tacaggttca atcccgacga cagccgcagg aagatgaacg
    24601 tcatcatggc cggcatcatg tacagcatcg tcgcggtctg cggattggcc ggcatgcctc
    24661 gctggccgat cttggacagc gcgaacatcg acagccccat gacgatcggc agaatgaagt
    24721 agggatcgaa gcgcgacagg tcggggagcc acaggaacga agagccccgc agctcgatca
    24781 tgtactggaa cacgaagtac agtgccagca gcatcggata cggaatcagc atcggcagac
    24841 aaccgccgag cgggttgacg ttgtgctcct tgtagatctt gagcgtttcc ttctgttgcc
    24901 gctccgggtc attcttgtac ttggtctgga cttccttgag cagcggggcc accgcctgca
    24961 ttcgcatctg ggacgccatg gccttctggt tcaagggcca gagcaccagg cggaccagga
    25021 cgccgaaggc gatcaacagc cagccgtacg ccacccccag ggactcgtgt cccgcgagca
    25081 ggatgttcgt gaccaggatg gagacccacc ggatgatggg ccggaaaatg gcccacccat
    25141 acggattggc atcatccatg tcatgcccga tcgccgcgag acgccggtgt tcgaggggtc
    25201 ccagaaagac gctgtactgg aaatccccgg ccggagggac cggcaacgtg agcgagacgc
    25261 tcatctccgt cgcgattccc gacttcccgc cgaagaaccc tttggcgctg tccggcctgg
    25321 ggcctcccac ggccaccacg ccggcgaact gagcctcgcc cggctcggtc gcgagcgccc
    25381 ccaccaggaa gtatttcgac ttgatgcccg cccactcgaa aggcccgtcg aacacgctcc
    25441 gctcgccctc ggtcaccgac gagaaggcgt gcttctcggt cttcgaagac ttggtgacga
    25501 gcgcgtagtc acggaacgag ccgctcgagt cggcttccga gttgcgcagc ccatctccca
    25561 gactcacgag catctgggcg ccggaaggcc cgagcccggt cacccgccct ctcacgccga
    25621 cgcggtagtc gtcggggtgg aaggtatacg cgatggtgat ggtcgaagga ccgcgagtgg
    25681 cggtgagggt cagcgtcgcc ggatctccgg caacgctcat cacgcgggtg gacggcgcaa
    25741 aggagaggtc agtcagatcc agggtgtctg agccgatggc gatgcggcgc cccagaattg
    25801 gtctgccggc cgggacgagc tcgacctgac gacccgagtc gcccgcggca tatgacttgt
    25861 actggctcaa gcgcgcggaa acgatggtcc caccccgcgt gctgacgccg accttcaaga
    25921 gcggagtcgt cacccacacg gtgtcagcgg gcgcagacag acggctagac ggcttgacgg
    25981 ccagctggtt cggaggagcg ccggccctgg aggagtccag gcggctcgaa tcgacgaccg
    26041 cggcagcctg ggacaaagtg tccgtctggc cgtctacccg tttaggtgtc ggcttcgggg
    26101 gccaaatgag actcggaacg atgatgacca gcgcaaagca caccagagcg atgaagacgc
    26161 gtcggtccac agatattcct tcgaccaggt cgccttacgg caccgggtcg tacccaccgg
    26221 gatgaaaggg atgacaccgc gccacgcggc gaatccctag ccaagacccc ttcagagcac
    26281 cgtaccgctc cacagcctca taagtgtaac gggagcaggt cggggtgaag cgacactgcg
    26341 tgaacaccag atgcgacagc gtcagctgat acacccggat gaggcccatc acgatgagac
    26401 ggggtatctg aagtacgttc acttgatctt cgcggctgcc tggttcagtt cgtcccgcag
    26461 cacggcgaaa gacgcaccgt aggcttcacg tcgtgcccgc accacgaggt ctacgggggg
    26521 caggagccgc agggtttcgc ggcgcagaat ctcgcgaatg cggcgccgca actgattgcg
    26581 ctgcaccgca gtgcgctggt acaggggaac gatgatgccg gagcgggaac gtgccggaag
    26641 ggtacgggga agccacgcga gctcgagatg cggcaaccga acgcgtttgc ctgtgttcca
    26701 gcaggcgtgg agttccggac cgcgccccaa gcgcgcgcgg cgcggatgcc gctcgctcgt
    26761 taccgactcc ggtgcttgga agcgatgcgg atcgtgagac gcttccgccc ctttttgcgg
    26821 cggcgggaca acaccgcgcg accccacttg tccttcatgc gggtgcggaa accatgctta
    26881 ttgatgcgcc gccgatttcg cggccggtat gtcggaccca ttgtgacctc tcgtaagggc
    26941 gcaaaattta cacggggcgc gcgagcgggg gcaatggttg acttttccac atagcgcccg
    27001 tactttcggg ccgcccttgc tgaactccca ccattttttt caatcgtgat ggagcagtcg
    27061 gccaaagaag cttggaagcg cctcctcgaa gaggcgcgac gggaactacc cgactcgatc
    27121 gtgcgcacgt ggctcgaacc tgctgagccc atcgcgctcg acgaggggcg cctgattgtc
    27181 ggcgctccgg atcagttcgc cgtggaatgg aacgaatcga aacactcggg attgctcgcc
    27241 cgggccgcgg agcgcgtcct gggtcagccc accaccgtgg tgttcaaagt ccaggacgag
    27301 cggcagcacc gcccgcaaat ggacttcttc gtcgccgcga aggacggcca gaccgccgtc
    27361 accgagcgcc ctgcctcgac ccagcccctc aacgagcggt acacgttcga gagcttcgtc
    27421 gtcggcaagt cgaacgagct cgccgccgcc gcggcgcacg cggtggtcga agcccccggc
    27481 ctcacgtaca accccttttt catctacggc gccaccgggt tgggcaagac ccacctgatg
    27541 caggccatcg cacacgccgt cattgcccgg aaccccggga cccggctcct ctacgtgggc
    27601 gccgagcagt tcatcaacga cgtgatcgaa agtattcagg cccgcaccat gccggagttc
    27661 cggcgccgct accgcaacga gatcgacctc cttctggtgg acgacgtcca cttcctggag
    27721 ggcaaggaaa tgacgcagga cgagttcttc cacaccttca acgccctctt tgaaggccgg
    27781 aagcagatcg tcctgacgtc ggaccggccc cccaaggaaa tccccggcct cgaggaccgg
    27841 ttggtgtccc gcttcgaatg gggcatggtc gcggatatcg gacagccgga tctggagcac
    27901 cgcatcgcca tcctccggaa aaaggcgcac caggaccacc tggagctcac catcccggac
    27961 gacgtgctgc ggttcatcgc cgagcacgtg cggtcgagcg tccgggagct cgaggggtgc
    28021 atcatcaagc tcctgctctt cgcctcactc aagaaccggg aggtctcgat cgacttggcc
    28081 cgggaagcgc tgtccgacaa gatccgccag ggggacgacg ccgggacgtt caacaaccag
    28141 gcggccccca gcatcgatcg ggtacaggaa gtcgtggccc ggcgttgggg cgtgacaccc
    28201 gagggactcc gctccaaggc ccggaccaag actctcacca ttccccgcca ggtggccatg
    28261 tacctcgcgc gggacatgct gggcatgcag ctggtcgaga tcggtcaggc gttcgggggc
    28321 cgggaccatt cgacggtcat ccacagcgtc gacaaggtcg agcggcaaat gatgcgggac
    28381 cggatcttca aggagcggat cgaaagcgcg cggaccgaat tatccgcatt gtagagaatc
    28441 aggatgtggg gatgttggcg agtggggatg tgggacgttt cgttcacaat gagccaacag
    28501 tcccaacatc gagccaacac ctgtggacag ggaaagcgca ttgagacgcg agagttgcgg
    28561 attccttcca catatcccgc tctctactac tactactagt ttctatatat agagaagtaa
    28621 aagcttcttt cctggggaaa aatgaagttc acgatcacgc gggaccagct gcaggaagga
    28681 ctcacggcgg tggcggcggc gattccggcc aagaccaccc tgccggtgct ctccaacgtc
    28741 ctcgtggagg caacgaagca gggtgtgcgc ctctccggaa ccgatctgga catcgcggtg
    28801 agtacgatgg tctcggcgga cgtggatgcc gaaggggcag ccaccctgcc ggccaagaag
    28861 ctcgtggaca tcgcccgcga gctccctccg gcagccattc gcttcacggg ggcttcggag
    28921 cagcgggtct cgatcgagtg cgggaagtcc cggttcaagc tgctgggcct tccccgcgag
    28981 gaattccctt ccttccccgc cgtgaagttc gatggcgcgt ccaaggcagc ggctggagtg
    29041 gtgcagaagc tgatcaatca cgtagccttc gccgcttcga ccgaagagag ccgccccatc
    29101 ctgaacggcg tgctctggga gctccgtccg gaccgcatgc ggatggtggc gaccaacggt
    29161 caccgcctgg ccaaaatgga cgtcccgtcg accggctcgg ccgctgccga cctgatcgtt
    29221 ccccccaagg cgctggatca gatccgccgg ctcttcgcgg ccgacgccga gatcgagatc
    29281 gcgaagagcg acaaccacat cgggttcagg tcgggtggga agctggtctt ctcccggctc
    29341 atcgaaggcc cgtaccctcc gtatgagaac gtcattccgc gcgagaacga caagtccgcg
    29401 accgtggatc ggaatgcgat ggcgggagca cttcggcgga tgagcgtcgt ggcgagcgat
    29461 cagacgcacc gcatccggct cgcgctctcc ggcgggacct gccggttctc ggtgcagact
    29521 cccgacctgg gcgaagccca ggacgagctg gccgtgacct acgaagggga gccgctggaa
    29581 atcggcttca atgccaccta tctgctggaa atcctgaagt tcatgcccac cgatgaagtc
    29641 aggttcacgt tcaaggctcc ggagcgggcc gcgacggtcg agccggtacg ctggaacgac
    29701 ccggcgagct atatggcgct cgtgatgccg ctgaggctcg tcgactagaa tccgccgcac
    29761 tgtcggtctt catggtgtcc gactttgtcg agtcgactgg aatcaccacg gggattgagt
    29821 ccagctccgc ctgcggaatc aacggcatca gcaatgagtc ggccgcggtc gtgtccggcg
    29881 ggatgtacac gtcgacgccg cggaatcgcg cgagaatcaa gtctcgacgg gcgagagtga
    29941 tcgccgggtg gaagagcgga ttcgatgacc gcggatgcgg cagcgtcgcc gccagctcgg
    30001 ccgcctgttc gtcactcagc cccgacggcg ggactccgaa gtagtcccgg ctcgcctggt
    30061 ccacgcccca cacgcccggt ccccactcca ccacgttcaa gtacagctcc atgatgcggt
    30121 ccttcgggag cgccagttcg agacgcaccg cggtgaccgc ctccttgagc tttcgcagag
    30181 gattccggga gggtgagaga tagagattct tcgcgagctg ttgggtgatg gtgctggccc
    30241 cgcggatgcg gtcgcgccgc ctccagaccg cctgcacggt cggccagagc ttcgaatgcg
    30301 gggtcagttg gagggcgtcg gcgatttcgg cgaagtcgat gccgtggtga gtcttgaagc
    30361 gggagtcttc cccgatgatc accatgcgct gcatgacggg tgcaatcgag gacaggggca
    30421 ccggatgcac ggtcgggagc cgggagcccc taaaggactc cccgctggtc gcgggagtcg
    30481 tcgactcctc gcgcatcatc gccgtcgtac gcggggtgtg aaagcgccac cagaccggtg
    30541 gtggccacac accgaggagc caaatgaggg ccaggaccgc gagaccggcc gcccagcgcc
    30601 ccagcctacg acgcatggtc ggctagtgcc tccgggtggg gagccgcgat gacacgccgt
    30661 acagcgtcac gtcattgatg gacgacgcat tccccggcgt cgtggccacc agtcccattt
    30721 gactgagccc accgatcgag aacccgaagg cgaactgggt tcgccggaat ttctggtcgg
    30781 tgtccatccg ggcgacgaac cccaggggga agccggggtc gaaggcggcg cgcaggtcgg
    30841 cgccgaaggc ccggaatccg gatgggttgg ccgaggtgag tgcgccgagg cccaaccccg
    30901 gggtgacctg gagattcgcg cttgccgtgt accggactcg ctgcaccagt ccccggacat
    30961 tcggctggcc gatgttggcg atcactcccg ccagcgccaa cgcgccggac gcccggtacg
    31021 tcatgccgac gtcgaacccg gtgcccctcg taccgccgcg ataccaggtg gtccccagcc
    31081 ccaccgccat cggtccttgg ccggtgccga aggcgagctt gtacgtgccc gcgctggtcc
    31141 cgccggagaa ttgatcgcgc tgatacgcga agcccaggcc ccgcgcattg aagccgacgt
    31201 tcagctgccg caattgtccc agtgaccccg ggttcgcgac ggtgaggtcg aagtagacag
    31261 atgcctcctg ccgcacggcg aggcctgagg gggtcaccca gatggcgcgg gcgtcctgtg
    31321 cttcagaggg caagatgtat gtgtcggagc gattcacggc gacctgagcg gacaatcccc
    31381 cgcccaggac gaggaggccg aagcccgcgg caaccgcgcg cttcagggca acaccacgat
    31441 cgtcgtgcgc gagacctggt gtcgcggaat cgccatgccc tgcggatcga atgtgatcac
    31501 gatccccgcg ctgtccacgg tactgccgcc gaggaagcgc cccgccgcgg agaactgcag
    31561 cttgccggcg tgggtgccgg tgccggaaag cgcgaaggtc gctcccccgg ccgctccgga
    31621 gcccgagacc gtgaagtcac cggagacttg tatccccagc tgccgcaccc ccgcgagact
    31681 ctcccactcg ccggtcgcgt agtgactgac ggaacgatcg accaccacgg cggtgcccgt
    31741 agtgtcggtc gcttcggtcg aatcgctcca cttcaccccc ggcgcgactc cggccgacgg
    31801 cagcctcggg aaaaaggtcc ggaatccgcc gaacagctgt gccaggttct tggcgatggc
    31861 ggcatccgaa ggtcgaggat cgatcagctg cccccagggg gagagccggc catcataccg
    31921 gagtccgcgg gccgactgaa ggtcgaccgt cgggggcagc accgtcgccg agtcggcgag
    31981 gatcgaatcg atggtgatcg agaccgagta cccgcgggca tcggccggac cgatgatgat
    32041 tgcggtgacg taggtcctga gaccgatctc ctgaacctgg tcgccggtgg gcagctgctg
    32101 gtcgacgtgg tgacgctggc tgatgacgta cttcgtgacc cccggcgaat acctgagggg
    32161 cgctgattga ggaatcgcgg gggtggctga cgccgggact ggcggaccac cggctgtcga
    32221 agtgggtgcg atggtgccac cgcaagccac ggcggcggcg gtcacgaccg cgagggccga
    32281 cactgaaatt cgcatcacgt tgtagcccca ctgcggtcgg ttttggtccg cccgaagacc
    32341 gccaccatct cctgcccttc ggacgacacg cgcagcaagc tccacccccg gcgccgcatg
    32401 tcggcgcgcc aggcgatgag cttcgaccat tcttcactgg ttgtccggaa tacccgcaag
    32461 tcggcgaccg actcccattg cgggggataa ggcttgagag gaacgggggt tggtggggcc
    32521 ataagccgag ttctgtttga ccgcctttcg acggtcagat ggtcatctct ctgggatggc
    32581 tgtcgccagc cacctcgtgc ggcctacccg cagctcgaac ggcccgggac cgagcctcgc
    32641 tgcttatttg gccttgctcc gactgggggt taccgtgcca cgaccgttgc cggccgcgcg
    32701 gtgggctctt accccacctt ttcaccctta cccctggata gggggcggtc tgttttctgt
    32761 ggccctttcc gtcgcctcac ggcgcccagg cgttacctgg cagtcagccc tgtggagctc
    32821 ggactttcct cggagtcgcc cgaccctgca gcgacccgcg accatcccac cccaccaata
    32881 cccgtccatc aagaagctat cggttctgag gctcccccgg caaggcggga gccctcaact
    32941 ccttatttct cgcgggctaa gcgacgcacg aggcgccacg tgctgtcctg tccggcggag
    33001 acgcccgacc gccgggcgtc gagccaaaac ccgctgttct cccggaaggc gagggggcgc
    33061 aaggctgccc agccggactg atcgacatcg cggggcacga gctcgaaggc actgagcaca
    33121 ttgatccccc ttccccgata gcgggcattg gtttgccggg acgacgggtc ccgggtgagc
    33181 aggatgacag gagtccccgg ggccgaccac ccccgcagca gtccccgccc gaaccagtct
    33241 tgtggcggcg cggctgcccg aaccgcgagc aggaggcccg ggcggatccg ctgaatcctc
    33301 gcgcgcaggt ccgaagcgcg ggtcgtggtg acccgctcga gcgcctcgaa cagatcgtcc
    33361 acgagcccct cgttcagcag cacgtcgtag cggtcctgcg gcgccacctt cttcagaccc
    33421 gcaccctggg ccgccgagat ggccgagtcc ttcaccatgc cgtccactcc ggcgtcgtag
    33481 tcggcctcgc agatcgtcgc ggcgggctgg gtagatgacg gctcgcgctc aaggtccacg
    33541 gccacggcgg ggatgagatc ctggtgctgc gcggcgagcc gggccagcga gatcatcccg
    33601 ggtacgaacg cggcattcca gtaaccggga tcgaggagac agcgtccggc ggcgacgccc
    33661 gccgtgtcgg gtggcaggcg gaacgagtca ggctcgaggg ccggcaccca gcgcgcgctc
    33721 gtctgctcca ggcggtcccc catcaccttc cacacgttct gggacaccgt tcgctcccac
    33781 ggcgcgtaat gcgtcgactc cgccacagct tcgacgtttg ccggtcccgc gaacacgttg
    33841 aaagccccaa cgtcgagcaa cgccgtcagc gaatcgaggg cgaccttccg ctccgcgtag
    33901 ccggtccggt atcccagcgg ctcggcccag agtacccgga gacccgcgcg accgatccaa
    33961 tcggggggcg tcaccgagtc tttcaccggc agatcggggc gcagcgggag cgtgaccgga
    34021 cgcgaacccc cgggcgggct cgcgagcgtg agccgggcgc gtacggagtc cggcccccct
    34081 tcgagggaca gcggcaccgg ctgcgtggcg gcggggacat tcgcccactc cgcaggacgc
    34141 ctggtccagc gcgcgaccgt ctccaggaag tccttggtgg tccccgcatc gtcggcggcc
    34201 agtgccgcgc gactggtgac gacgaccagt ccggccgcga cccgggtggc ggcgacgacg
    34261 acgggctgtg gggcgccccg agtcgccggg gtcacccgcg cgagcgcctg cgccggcagg
    34321 cgaatccgca acgtgcgcgc gtcctccagc tcgatcggta cgagatcagc actcggcagc
    34381 gtgcgacgga tcggcagggc gatgcgctgc gtgtcggggg gagcggagtc ggcgatcacc
    34441 acgcccgcgc ccagggactt gagccagcgg ttcatggtcc agcggtcgag tgcgctctcc
    34501 gggtacccga ggacgacgac gccccccgcg cgaatccact cggtgaggat caccgcgtcc
    34561 ccggacgtca gcgcgtcgga aggctcgtcg ggccgcaggc cgccgagcag caccacgccg
    34621 tggtagcggc gcatgtcgtc acgcgtgaga tgcgggaaga agcggcggta ctcgaccgtg
    34681 taaccggcat cgtgccaggg agcggccgtg gtctggagat cgacgggatc ggcgagcgtg
    34741 tagtcgagca gcaggagccg actcgccgtc gggcggcagg cgccgagtgt gagcagcgcc
    34801 aggagaggaa gaccacgctt ggtcacctgg gaaagataag tgcgggaagt tgaccaagtc
    34861 ttgatgcccg gggcgtagga tgcccccgtg gtgacggtct gcacggtatt gccggttccg
    34921 gagcgttccc gggtggacgc ggtgggggat gggtgtttca ccaccgtcca cgtggaaacg
    34981 ttccgcgaag tgctgcaggc ggcacggcgc cgaaaggtcg atgccctgct catttcggtg
    35041 caccggtgcg ccggagacga gatccccgcc gtggcgcggt tcgtccggga attcccggcg
    35101 attccggccg tcgccctggt ctcgcgtcac gacggcgtgg ccaccgaaac cttgctgcgc
    35161 ctcggggcca cgggagtccg ctcggcggtc gactgcaccg agccgggtgc gtggcgccgg
    35221 ttgcgcgacg ttttcgggca tcccatcacg ccggcagcag cccggatact cgctcgactg
    35281 gtgcccgcac tcggtgatgc gcccgaggag acccgggtgt tcttcgaggc ggtggtgcgg
    35341 ctggcgcccg tcatcgtcac cgtgcgcggc ctggcccgcc atctgcgcgt cacgccgagc
    35401 acgttcgtgt cacgctttca ccgcgccacc ctgccctcgc ccaaacgcta cctctccgcg
    35461 atgcggctgc tccacgcggc ctatctgttt cagaatcccg gattgtcggt gtcggatgtg
    35521 gcgtatcggc tggacttctc gtctccacag agcttcgggc ggcatctcaa ggcgatactc
    35581 ggcgtgacgg cgggagagtt tcgaagccgc tttccgttcg aagtggcgct cgcacgctac
    35641 gtcgatctgc tgatcacccc gtaccgggag gccctgcgcg cgttctaccc cttaaacgcg
    35701 gggttttggg accaaggatc cgacgtgcct cgcgtctccc gggcggggtg aatcgcctca
    35761 gtcggcggcg gcgcggacca cccggtgctc agccgtcgac tgctcgggct tgaggacggc
    35821 gaagcgcgcg atactggcgc gcaggcgctc ggcgagctgg gcgagctgct gcgacgtggc
    35881 ggtgaggtcg cccatcgaag tgatctgtgc ctgcgtggcc gatgccgcac tggtggcgtg
    35941 ctcggcggag acgccggaca ccgtggacac ctgtgcgagc gattgcgcga gctgcgcgag
    36001 ccgctcggct tgagttcgcg agacctccgc ggtcgcgtcg acgaggccgc cgattacctg
    36061 aacgccctcg tggagctcgc ggagcgcccc gtcggcctcg gccgcgacga cgccgatgtc
    36121 ccgcacctga ctctcacccg tttccatggc tcgcgcggcg gccgtgatgc cggtgcggat
    36181 ctcgctcacc agctccgcga cctcgcgcgc cgacttccct gcctcgcccg ccaatccccg
    36241 gacctcctgt gcgacgacac cgaacccctc gccatgctcg ccggcgcggg ccgcttcgat
    36301 cgccgcgttg agggcgagca agtgggtttg ccgcgcgatc cgggcgatgg tctgcgcgaa
    36361 ccccccgatg cggtcggaca tggccgaaag gccaccgacg gtcgccgccg tggcgcgcac
    36421 ctcttcgcgg accgagcgca gcgtctgact ggcgcgctcg acgcggtctc ttccccgcgc
    36481 cgccgtcgac gcgagctgac cggcgtccga ctgcatccgc tccgcgcggg tcttgaggct
    36541 ctcggcctgg gccgccgcct ttgcgctctc cgcctgcgcg ccttcggcga cggtccgttg
    36601 cccgacgaga tcgtcggcga gtcccttggc ggtctgggtg acggcctcgg acgtcgcgtg
    36661 aatctgctcc gccgcggcgg cgagctcttc ggcgaaggcc gcgacttcgt ccgcttcatg
    36721 ctgcaccgtg gagatcgtcg cgccgatctc gtccagcatc cggttcaagc tcttttccag
    36781 gaatccgagc tcgtcggact cggcggcggc ggcgcgtaca cccagctgtc cctgttcagc
    36841 ggatcccatg atgctgcgcg tcgcgcggat tcgggtgatc agggtggcgg ggatccgctt
    36901 cagggccatg gcgacggcga cgaagacgac ggtctcgagg tacgcggccg ggggaatgtc
    36961 cgcggagccc tcatacagtg agccgtggag catggtggac gcgaggtatg cgagggatcc
    37021 gacgagtacc aggaagtccc cgacggtgcg gccctggtcg aatgtgtagg gcaggaccgc
    37081 gatgaagaat ccggcgatga ggccgccatg tccgaaccac accaccggca gggccgcgag
    37141 gagcacgtcg aggagggtga ggacgtagat cagccaccag cgataccacc cgcgctcatt
    37201 gacgagtcca accagggcgt tgagaatggc gccgccaccg gccgccaaca cgatcgtccc
    37261 gggtgacgtg cggatggtgc cgctgagcca gcccaggatg ccgacgaaga acacgccgga
    37321 caccatgacc cagcggcggg aaagggagcg ggcgaaggtg tcgaggtcct tgaggcgctg
    37381 ctccagcccg gcggagtcgt cggagcgccc gcgcagccag ttggcgaggg accgcttctc
    37441 gggatggagg gaagggatcg tcacaggccg agccccttgg ccgtgagaag cgtctcgacg
    37501 gcatgccccg cgcgttcgag cacctcccgc cctccttctt cgcggtcgac cacggcgaac
    37561 acccccagaa tcttcgcccc ttccgccgag agggcggcga tggcttcctg ggctgagccg
    37621 cccgtggtga tcacgtcctc taccaccacc accgattgtc cggcttcgaa gcagccctcg
    37681 atgcggcgtc ccgtcccatg ggtcttggcc tgcttccgaa tggtaaacgc atcgacgacg
    37741 ggggcagggg accggttgcg ggaggccgcc gcaatcgcgt acgccaccgg atcggccccc
    37801 agggtcaggc cgccgaccgc ccggggagcc caccctcgag ccaccaaccg ctcgacaccc
    37861 agggatccaa tgagagccag cccctcggcg gacatcgtgg tccggcgggc gtcgatgtag
    37921 aaggtggact tacggccgga cgagagggtg aagtccccgc gggtgaccga gcgggaaagc
    37981 aggagttcgc ggaggcgatc gaggtcagtc atttgtgttc gtagtagtag ttataactac
    38041 tactatcatc tcatttcctg ccgacgatgc tcttcagcgc accgaagagt cccttggacg
    38101 gtgggggagt cgggtcagcg gcgaggccct gctcgagctc ggccgccaag gcctcgacgg
    38161 tgggctggcg ggcaccaggg tcacgcgcga ggccccgcat gatcgccgcc tccagacccg
    38221 cggagaactt caggcccggc accgcctgat tgagaggctc cgggggctgg gtgaggagct
    38281 gctggaagag ctcccgcggg ctgcggccac tgtatggatg tcgtccggtg aggaggtaat
    38341 acgcgatggt cgcgagcgaa tagatgtcgg cgcggccatc cacgagctcg ccggaaaggg
    38401 cctccggcgc tacgtattgc agggtgccta cgaagaagcc ggtccgcgtg agacgctcct
    38461 cgggcggcac ttccgtatcc cgcgcgatcc cgaagtcgag caggcgggcc cgcttggtgg
    38521 cgggatcgta catgatgttc tcgggcttca agtcgcggtg aatgatcccg gcgtgatggg
    38581 ccgcggcgag tgcgtccgcc agctgccgaa ccagggccac ggcctctgga ggagcgagcg
    38641 gtccgttgcg gtgtaggaat gccgcaagcg agtcacccgg ggcccactcc agcgccaggt
    38701 agtacaggcc gtcggcctcg ccgtagtcga acgttctcac cacgccgggg tggctgaccc
    38761 gcgcgccgta tcccgcttcc cggaggaagc gtttgacggc tgtagggtcg ccgcgtagcc
    38821 gggggcgcaa taccttgaac gccgacggcc ctcgctcggc gtgctccgca cggtagactt
    38881 cggcggtgcc gccttcgccg acgcgctcta gaaggcggta gccggcgatg gtttggccgg
    38941 cgagattctc ggtgggcatg aggacgcgcc aaactaacgc ccggatttga ggtggagcaa
    39001 gtgatcagac gggtcgccgt tcccgtggtc ctcggaatgt cgctggtctc gtgcggccaa
    39061 tggtcgcgcg tcggctcgcc tgacaatccc aaacccgcgg tacaggtctc acgcctcttc
    39121 gacgccgctg cccagtacag gagcatgggc ctcctggccg cgggtgatcc gttgccctgg
    39181 gtcggggcgg tccgctggct ggccgggtcg acgcccgact ccacgctcgc cctcgtctcc
    39241 ctctcattcg ccaaccattc cctccgcttc cggagggacg gcaatgactt cgtcgcgtcc
    39301 tatcgggtgg agctgtcgtt ccacggcgcc aacggcgcgc cgcgcaccat cacccgggat
    39361 gaaacggtcc gggtcaggag cttccaggaa accctgcgtg ccgatgagag cgtgatcttc
    39421 cagcagttcg tctccgttcc tccgggcccc tgcaccatcg ccatcgtggt ccgggaccgg
    39481 gacgcggtag gcgcctcgcg gcaggaagtg gtggacacca tcccgagctt tgcgggtccg
    39541 accctctcga cgccgatccc ggtgtacgag ggggcagggc ggtcgcgccg ggacacggtg
    39601 cccgccctcg tgagcaatcc ccgcgcgaca ctaccgtacg gaaacgacac gctcttcttc
    39661 tacgtggagg cctacaatct gccggccggc aacccgatcg cggccgcggt actgagcccg
    39721 agtggcgaca cggtctgggc cgatacgctt cgcctcgacc cccgggaagg cgtggcccga
    39781 accattttca gaattccgcc cgccaaactc cccgtgggcc aggagcgctt ctacgctgcc
    39841 gcggtgggga cgggcaaggg cgtacaggcg cccttcctgg tcagcttctc gtcgcaatgg
    39901 gtcgtgacga acttcgacga gatgctcagc ctgctgcggt tcttcccgcg ccaggacctc
    39961 gtcaccaagc tgcgttcggc acccgattct ctgcgtcccc aggcctggcg ggacttctgg
    40021 aatgcctcgg accccgtggc gatgacgccg gaaaacgaag ccttgaacgc ctacttgagc
    40081 cgcgtgcagg tcgcgagcca gcgcttcacc gaatcggccg atcccggctg gctcacggat
    40141 cgcggcgagg tcttcatcac ccttggggat cccgacgagg tctacgatct cgagagcgac
    40201 tattcgcgga ccggactgca tgggatccgc tggatctaca cgggtctccg cctcgacttg
    40261 ctcttcctcg atcagaccgg gttcggccgc tttcggctaa cgccgggatc ccgtgcggat
    40321 ttcgaccgcg cggcgacccg gctgcgtcac gagaactgat gcgccgcgcc gtctgggcgc
    40381 tttcgctgtg ctggccgttg gccctgccgg cgcagaagcc ggcggaggtc gcacgcgcgg
    40441 aatccctcga aatcgcgggc cgtccctggc acgccgctga ggcattcgtc gccggggccg
    40501 gcaccgcggc cgcgccggcc tccgctcaag tcctcatcgc cgatgcccgc gccgagctca
    40561 ccgctcgccg gtacgcgcgc gtcgtggccc tgctcgacaa gcgctcctgg ctcgccgatt
    40621 ccggggacgg ggtcggctac gccatcctcg cggaggccga ggatcggctg gggcgctccg
    40681 cggacgcggc gcgggactac ctccgggcgc gaagcctggc caccgggagc cgggccgcgc
    40741 tgctcgcggt ccgggcggcg gtcgtcttcg atcgcgccgg ggcggacgac agcgcccgct
    40801 tctactatgt cgcggcccgg gcggccgggc ttccttccat cgacgaatgg ctccggctcc
    40861 gcgaagcggg tgtgagccgc gataccacca tcgtcgactc gttgctcgat ggcctgacgt
    40921 tccccgcgtc tcgacgggct ccgctgtatc gagcccgcgc cctgctggcg gcaggggact
    40981 cgggactggc ggtggacgct ctcgcggcgg cgcccggtgg tgctctccag gcagcggagg
    41041 tcgccctggc ccgaagggac tcggcctggg cccgcaagct tctctatcat ctctttgcca
    41101 cggcgcctca atccgacgac gcggccgcgg gagaagccct cgcgcagggc ccgctgccgc
    41161 cattctcgtc ggcggaacgc acctcgctgg cgcaggtcct ccgcactcac ggtgacgcgc
    41221 cggcggctct ggtccaggtg cgacgggcgg tagcgtccgg cgactccagt gccgccacgc
    41281 tgatcctcct gggcgacttg ctcgtgagca accgcaagga tcgggacgcg ctcaaggcgg
    41341 tgtccgcggc gatcacgatg gccacggact ccgcgacccg ggccctcgcc acctaccggc
    41401 gggcgcgcgt gctcgtccgg atgggtgatc ccggcgcggc tcaggcgctc tccgactatg
    41461 cccgcgcgta cccgggagac agctcggcgg tgacggcgct gtacctggcc ggggaccggc
    41521 tcgccgagca gggagattcg acgggtgccg aggcgatgtt ccgggagctc gtctccgccc
    41581 acgccctgga cgccgcatcc gttcaggcgc gtctccgcct cggctcctgg gagtaccggc
    41641 gccggcactt cgatcgcgcc gccgaatggt tcaggggaga ggtcgccgcg agccccgggg
    41701 tccatggcgc ggcgcgctac tggctcgcga aaatcgccgc ggcgcaggga gacacggcgg
    41761 atgcgagggc gcagtggacc gctctggctc acgacgactc agtcggctac tacggactgc
    41821 gcgcccggac cgcagccggg ttgccgcccc tgacggtcgc ggaaggcgtg gattccaccg
    41881 taccggccgt gaccaccgga ctggctcggc tcgacaccct gcggctcgcc ggtctcgact
    41941 ccgaagcgga cgcgcaggtg cgcgcgctgg tcgcgaatcc ccccgactcc gtcgcccagc
    42001 tgctcgcgtg gacgtcgggg ctcgcgtccc ggggatgggg atcactgtcg gtacgcctcg
    42061 cgtggctcgc cgctccgcgc gctcccggga accccagggt gctgcgcgcc atctacccgt
    42121 ggccgaatcg cggggcggtg gtcgccgagg cgaacgaatt ccacgtcgac ccgttcctgc
    42181 tcgccgcgct gatccggcag gaaagcgtat tcgatcgtga agcgttgtcc cgcgtcggcg
    42241 cccgcggcct cacccagctc atgccgagca cggccgcgta cacggcgcgc ggtctcgatc
    42301 tgcccttcac ccccgaatgg ctcaccgtcc ccgacttgaa cctgcacctc ggcgccgcgc
    42361 atctggggac gctcctcgcg cggtaccacg gccgggtgga tgccgcggtc gcctcctatg
    42421 atgcgggcac cacgcccgtg gaccggtggc tcgcccgcgc gccgggcgcc gatcccgatc
    42481 tcttcattga agacgtcgcg tacgtcgaga cgcgggggta tgtgcggtcg gtcctgagga
    42541 actgggaggt ctatcgcgcg ctgtacggcg cgaactgaat cggacttaga ttcccgccat
    42601 gcccccccgg tctaccgatg cgctttttct gatcgacggt tacgccctca tctatcgcgc
    42661 gtttttcgcg atgatcgcgc gtcccctcac tacacgtcac ggcgagaaca cttccgccgc
    42721 gtggggggtg acgaacttcc tcctccgctt gcgggagcgg caccaacccg ggtacctggg
    42781 atggatcaac gaccgggggg tctcgttccg cgagcggttg tatcccgcct acaaagccac
    42841 ccgccagaag ctcaccgccg agctccagca ggatttcgac cggtcggtgg aacgcatcgc
    42901 ccagctcctc gaggcgttcc gcgtccccct ggtcaccgtg gaaggattcg aagcggatga
    42961 cgtgatcggg accctcgccg ccaaggccac ggaccagggt gtgctctcgg tgatcgtctc
    43021 gggggacaag gacttctatc aactgatcgg acccaaagtc gccctgctca accccggccg
    43081 ctcgggtccc gcgggagtcg aggaacagtg ggtggacgag tccaacgcgt cggaacggtt
    43141 gggagtgccg ccccggcagg tgatcgacta cctggcgctg gtgggggaca gctcggacaa
    43201 cgtccccgga gtgaagggga tcggcgagaa gaccgcggtg gagttgctcg cgaagtaccc
    43261 cgacctcgaa acgatcatct cgcacgcggc ggagatcccc gggaagcgag cgcgggaggc
    43321 cctcctggcg cataccgagg aagcgcggct ttccagggac ctcgtgacca tcaggcgcga
    43381 cgtaccggtc accctcgacc tcgacgccct gcgggtgaaa cccttcgatg ggccccggtt
    43441 gacggagctt ttcaccgagc tcgagttccg gtctctcatc gcgaaggtga ccggcccgga
    43501 cgccgcggtt ccgattcggt catcggctcc ggcggcagag cctgccgccc agcccgctcc
    43561 cgcgcccgcg gcagctcccc aggccgtgcc ggccgttgcg gccgtcccgg cgcgggtcct
    43621 cgatgacgtc gcagggctcg ccgcgatgat cgccgaatgc cggcgcgctc cgatgatcgc
    43681 gctcgacacg gaagcgtcgg ccatcgatcc gctgcgggcg tcgctggtcg gactgtcgat
    43741 cgccgtcgct ccggggcagt cctggtatct gcccttcacg caccgccccc cggacgggga
    43801 gctggccggt ggagtccctc cgaagaacct tcccgcgatc aagagtcccg agctcgcccc
    43861 cctccgcgag ctgctcgccg atcccgcggt gaagaaagcc ggccacaaca tcaagtacga
    43921 ctggctggtt ctccggcgcg ccggtgtcga gctggcgggt gtggcctacg actcgatgct
    43981 cgcgagcttc gtgctcgacc cgggccgccg gtcgcacgca ctggacgcgc tcgcgctcga
    44041 acgcctcaac cttccgatgc gcacgtatgc ggacgtcgcg ggccgggggc gcgccgagcg
    44101 tccttttgcc gacgtgcccg tgccggacgc ggcgcgctac tgctgtgacg attcggagat
    44161 ggtgctgcgg ctggccgagg cgttcaacgg ggagctcgac gaccaccacc tgcggcctct
    44221 gctcgacacc atcgagatgc ccctgatccg cgtcctggcg gacatggaat gggccggcat
    44281 ccgcgtggac cgggagctcc tcgcgaacct gtcgcggcag ttcgcgatcg acctcgggcg
    44341 cctggagaag gaaatccacg cggcggcggg aacggacttc aacatcgcgt ccacgccgca
    44401 gctccggacc gtgctgttcg agaagctgca gctgccggtc ctcaagaaga cgaagacggg
    44461 ggcgtccacc gactacgaag tcctcgaaca gctcgcgggg atggggcacg aagtgccccg
    44521 gctgctcatc gagtaccgcg agctctccaa gctcaagtcc acctacgtgg actcactgcc
    44581 cctgaacatc aacccggata ccggccggat ccacaccagc ttcaatcaga ccggcgcggc
    44641 cacgggacgg ctgtcctcat cggatcccaa tctgcagaac attccgatcc ggaccccgcg
    44701 cggcgaagag atccgccgcg cgttcatcgc gtcgcccgga agcgtgctcc tcaccgccga
    44761 ctactcgcag atcgagctcc ggctcctcgc gcacctgtcc ggggatccgc tcctggtgga
    44821 agcgttcgag cgcggaggag acatccaccg gcagaccgcc gcggtaatct tcggcgtgcc
    44881 cgacgatcaa gttacgtctg aaatgcggag ccgggccaag accatcaact tcgggacgat
    44941 ctacggccag ggacccttcg cgctcgcgcg gatgctgtcg atcacccagg acgaagcgaa
    45001 gcgattcatc gcggaatact tccagcgctt cgcgggagtg cggacctggc tcgaccggac
    45061 cgtcgcggag gcccggcagc gcggatacac ggagacgatt ttcaagcgcc ggcgatacat
    45121 cccggagctg aaggacaaga acttcaacat gcgggcgttc ggggagcgca cggccacgaa
    45181 ctccccattg cagggctcgg ccgccgacct gatcaaggtg gcgatgatcc ggatcgcggc
    45241 cgggctggcg gaggggaagc tcgggacccg gatgctgctg caggtccacg acgagctggt
    45301 gttcgaggtc cccgtggatg aacgggaggc ggcatccgtg ctggtgaagc gggagatgga
    45361 aggagcggca cggttgcgcg taccgctggt ggtcagcatt ggaaccgggc cgaactgggt
    45421 ggaagccaag gactagggcc gttcttgctg gaggacacgg ggtcctccac atttggcgcc
    45481 atgcgcccgc cccgcggctt cacgctcatc gagctcctga tcgtcatcgt gatcatcggt
    45541 atcctcgcca cgatggcgat cctcaactac acgagctcca agcaaaaaac ctacgaagcg
    45601 tcgatgaagg ccgatctgag gaacctggcg acggccgaag aagcgtattt ctacgacagc
    45661 tcctcgtaca cgaacagctt cgccatgatg agcaactaca cgccgagcgc cggcaatgcg
    45721 atcgtcgtca acgaggcgac ccgggtaggc tggtcggcca cggcgtcgag caccgggacc
    45781 ggccggaagt gctacctctt cttcggctcc gcgacgcccg tggggaacgc gaccaaggag
    45841 ggtgtggccg cctgctcctg acggagctga tcagaacgtt cgcgcgccgg cgtgaaggga
    45901 tgatctacgc gatggatggg ggcgtgtggc tccaccgtca cgtctttcac gacgagccca
    45961 tgattcacct cgtcagcacc cgccgtgaac cgctcctgct cgtcggtgac cgcctcggac
    46021 tcgatcccgc ttggctccag tacaagcccc tgaaagaccc ccggacgggt gtgcgggtcg
    46081 aagcctggca ttgggatttg tggggggaat cgaagggacg gctggaggcc ctgctcgaaa
    46141 cgggtcagtg acccgggggc ggatctttgg gggcccggac gcccggccac cagttccagc
    46201 ggccggcgat gtgcatgatc gcggggacga gcaccatgcg aatcagcgtc gcgtccagga
    46261 ggacggcgac ggccagtccg aaccccatga actgcaccgc cagcacccgt gaaaacgcga
    46321 acacgccgaa caccaggacc atgatggccg ccgcaaacgt gatcgtcgac gccgtgctcg
    46381 acaggccttc catcgtggcg tgatcgttgc gacccgtctt gtcgaacact tccttcacgc
    46441 gcgtcagcag gaacacctcg tagtccatcg acagcccgaa cacgaccgcg aagaccagga
    46501 ccgggacgac cacgaagatc gcctcggtcg gcccatccag tcggaagagc cagcccagga
    46561 cgcctcgctg aaagacgagg acggtgatgc cgaaggcgcc gcccacggaa agacagttca
    46621 gcagcacggc cttcagcggt acgagcaacg acttgaacgc gatgaagagc atcacggccg
    46681 tgacgcccag gatcagggcg acgatggtgg ggaagcggga caggaggtcg ttttggaggt
    46741 ccacactggt tgcggcgaat cccccgacca ggatcgtcgc tccatccaat ccgcggattc
    46801 ccttgcgggc cgtctgccgc acgttcgtgg ccatctgcat caggccggtg agcgtcaccg
    46861 agtcttcggg gatcacatcg accagcgtgg tacgggaatc cgcgctcagg taggcattga
    46921 agaacgtcgt atggtgccgg cggaccgaat caggattggc gtagaacatg gtgagcgcca
    46981 gactcgagag atgcggctgc aggctcgcta ccccgcgcac ctgtttcacc cggggatcct
    47041 tgcggatccg gtcccccagg gccttgagcc ccagcagacg gcgcgcggac aacgcggact
    47101 cgccgggagg gagcgtgatc acgacgcggg tcggttggat gatgcccgac acgcccatgt
    47161 tcaccagctc ctgcaggccc acgccggact ccgactccga cggaaaccag ttccgggccg
    47221 gcaatccgat cttcatttga gagagcggga tcatcagcag tccgatgatt ccccctccca
    47281 gcgcgatcgc gcgccagggg cggtggccca gccagcgggc ccaacgctcc cagccggtcg
    47341 gcgcgtgaaa tctcgcgagc gggcgggcga gccacttcgg gcggtcgatc ccccggccga
    47401 ggatcgcgag cagcgccggc aggaaggtcg tcgcgatgcc gatcgcgatc gcgaccacca
    47461 ggagcccgcc gatccccacc gaccgggtct cgatcagcgg ggtgacgagc agggcggcga
    47521 accccacgac cacggtggcc ccggacgtaa tgacggccga gccggcggtc tgaatggtgc
    47581 gggccgccgc gtccgccgcg gcgaggccgc ggttcagctc ttcccggaac cgcgtcacga
    47641 tcaagagcga atagtcgatc cccacgccaa gccccaccat gctcgtgatg gtgagtgcga
    47701 agacggacat ggtctggaaa tgcgccacga tggtgatcag gcccagcgca atcgtaatgg
    47761 cgagcacacc cacgatgatg ggcagggtcg ccgcgacgag cgccccgaac gcgacgacca
    47821 ggaccgcgag cgaaaacggc accgcgacgt gctcgccgtg ctgggtatcc tcggccgaaa
    47881 tcgtgcggat gtcgtaatcg agcgcgggat cgccggtgag cttgacgtcg aacgcgcccg
    47941 cgccgggaat gccggcgact acgccggcga gggtggaccg gatgtccggg acgaagttgt
    48001 tcttgatgtt gtcggtctgc tcggtctgca tcgccgcgat gaggaaggtc gtgtgacggt
    48061 ccttgctgac gaacgtcgat tcaccgagcg actgggccga tacgacctgt ttgatgtagc
    48121 ccttgcggga gagggcggcg ctgagggagt ccagcacccg gacgaaccgg gggtgattca
    48181 cgggtacggg accgtgcacc acgacggcga cgtactcggc gatgggattg gggagtgccg
    48241 ccttgagcgc ctctccggca cggaacgatt cggtggattc gtccgagccc ccgcgcaccg
    48301 cgagagcgtc ctgtacccgc gcggcactgg gtgccagcag cgccgccacc agggcccacg
    48361 cgaagaggac ccaccagcgg cggcggatca gcatcttggc ggggagcgcg atcactgggg
    48421 tgggcgcata gaccgaggac aatcatagag agactccaag gccgaagcta gcggcgcgcg
    48481 tgcgcgcgcg gacatattgc gcccatggcc gaacggcgtc cttcgatggc tctccaactg
    48541 ctcgccctcg ccgtcgtggc ggtggtggtt ttccgcttcg cggcccggca tcgcaccccg
    48601 cgcgcgccac tgccggggca tcgcgcgccg gtcgcgaccc tcccgccgag gcacgattcc
    48661 ctgggggctc aggtggcggc gacgccggac acggcgggcc gggatacgac gcccgttccg
    48721 cccatcaatc cgatggacac cctgaccatg aacgagccgc agggctacta cgatcagctg
    48781 gcgcgggcgc agacccggcg gcagatccgg gccagcgcag gcaccaccta tctgggcgaa
    48841 atcgtctccg agagctcgga ctccgcgttg caccgatggg accaccggta tgaggacccg
    48901 gtccgcatct atctcaccac cgacaccgtc gagaacttcc agccggcatt cgtggacgcg
    48961 gtgcgctccg cgctgacacg gtgggaccag gtgaacctgc cggtgcgctt cacgttcgtg
    49021 gacgactcca cgcgcgcaga agtcaccttc cggtggcgcc ggcagttcga catcgaccgc
    49081 acggggcaga ccgacctgac gtgggatcgc gacggacatg tgctgtccgc cgtcgttacg
    49141 atcgcgacct ttgatcccaa caatcacccc atgggcgtcg aagacgtgcg tgtcgtcgcg
    49201 ttgcacgaaa tcgggcacat gctcggcctc aatcactcga gcgattcgac cgacgtcatg
    49261 tacggccata ccctggcgcg cgacctgtcc ctgcgggacg aacgcacggc gcagctgctc
    49321 tacgagctga gccccgggag cgtccggaat tgagccggaa tccgctgccg ctcctcgcga
    49381 cctcgtcgcc agcgggtccc tcactggccg acaccctcga cacccggacc atgccggcga
    49441 gcctggtgac tcctgaggtc gacggggccg tacgctgcgt cgcctgcgga caccgctgcc
    49501 tggttcgccc cggacgacgc gggatctgca aggtgcggtt caaccgcgac ggcgtgctgc
    49561 gggcacccgc ggggtacgtc gccgccctgc agtgcgaccc caccgagaag aaacccttct
    49621 ttcactgcct gcccggcagt gacacactca ccttcgggat gctcggttgt gatttccatt
    49681 gcggctactg ccagaactgg ctgacgtcgc aggcattgcg cgatccgtcc gcgggcgtgc
    49741 cgcccaccga ggtaagtgcg gaggcgctcg tagggctcgc gctgcggcac ggcgcgcggg
    49801 tcatgggatc ttcctacaac gagcccctga ttacggcgga gtgggcggtc gaggtcttcc
    49861 ggctggcccg cgctcgaggc ctcaagaccg cgttcatctc caacggcaac gcgacaccgg
    49921 aggcgctcga ctttctccgc ccgtggaccg attgctacaa ggtcgacctc aaggccatgg
    49981 acccggcgcg ctaccgggag ctcgggggcg tgctggagca tgtgaccgac accatcgcgg
    50041 cggcgcacgc gcggggcttc tgggtggagg tggtcacgct gatcgtgccg gggttcaacg
    50101 acgaccggcc ccaactgaag cgggcggcag agctgatcgc ctcggtgtca cccgacatcc
    50161 cgtggcacgt cacgggattt caccccgact accggatgac cgaccctgcc gcgacaccga
    50221 ccgagacgtt gatcgaggct tgcgaggtcg gcgtcgcggc cggcctccgt ttcgtctatg
    50281 ccgggaatcg ccccggggct gtcgggtcgt gggaaaacac gtactgtccg gattgcggcg
    50341 acttgctggt cgagcgtcgt ggataccaga tcctggccca gcgcgtcggg agcgacggcc
    50401 gctgtccttc atgtgcccgc gccatacccg gcatctgggc ctgaatcccg cagtcatcgt
    50461 gctcgcgctc ttcacaggcc cgaggcctgt gctcggcgga gcactgcgct taagcgccgc
    50521 gcggtgccga gcacacgctt cagcgtgtga aatccaggcg agtgccagtg tctacgactc
    50581 catcgtcgtg tcaggcatca agcaaggggc gtatccaggt gccgtcctcg tcgtggggcg
    50641 ccgagaccga atcctctatg agaagggatt cgggcatctg acctggtcgc cgcgctctcc
    50701 ggtcgaccgc cccgactcga cgattttcga tctcgcctcg ttgaccaaag caatagcgac
    50761 aacgacggcg gccatgatcc tctacgaccg cggcaagttg aagctcgacg ttcccgtctc
    50821 gacttacttg ccggagttca atggcgcagg cactgcggca atcaccgtcc gcatgctact
    50881 gacgcacacg tcgggccttc gcggtgacct tcccgttccc gagctgcgcg cgctgcctga
    50941 cggcaccgcg ctgattcgga cggtgctcgc ggaaaccccc cggaccaagc cgggaacgcg
    51001 ggtcgtctac agcgatctga atttcgtgct ggtcggcgag atcatcaggc ggctctcggg
    51061 tgagcccctc gatcagttcg tcaccaccga ggtgatcggc ccgctggagc taacggaaac
    51121 ccggtatcgc ccctcgacgc gtctggcgtc gcgcatcgcg cccacgggag tctggcacgg
    51181 acatccggtc gcgggcgtgg tcaacgatcc cacggccgcg aaggcgggag gggtctcggg
    51241 gaatgccggg ctgttctcga ccggtgagaa cctggcccgc ttcgcccagt tcctgcttcg
    51301 cgggggcacg cgcccggacg gcaagcccct cgtctcgccg gagacggtga agttgttcac
    51361 gacgcgcgcg tcccaggccg acgtcggtcc cgaccaccgc gcgctcgggt ggcaggcggt
    51421 gccca
//
LOCUS       DASUBP010000033         2805 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_613959, whole genome shotgun sequence.
ACCESSION   DASUBP010000033 DASUBP010000000
VERSION     DASUBP010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2805)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2805)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2805
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613959"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..195)
                     /locus_tag="VFU75_01700"
     CDS             complement(31..195)
                     /locus_tag="VFU75_01700"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEU5465568.1"
                     /translation="MPLYEYRCTACDEVFTEQLTISEHGKRRPVCPSCGSRAVEPVLS
                     TFFAKTIRKS"
     gene            283..771
                     /locus_tag="VFU75_01705"
     CDS             283..771
                     /locus_tag="VFU75_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465569.1"
                     /translation="MLVLALVLGCNDGLQPTTICGPHLVGICGHITFRGAVPDSTDVM
                     YLVAYGSFPQTENDLFNFLPFPPQTLQIPLAGDSVVPYGLALPNNQYHWVLAVWKKVG
                     TLNPSDTASADALLREAGYYRDPADTTKPGVVTVHSAGTGGVDFVVDFNNMHSVSYFF
                     PP"
     gene            768..>2805
                     /locus_tag="VFU75_01710"
     CDS             768..>2805
                     /locus_tag="VFU75_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5465570.1"
                     /translation="MTSRLALAAALLIATPAAAQQTGTVVGTVRDTAGAPVAGAKVEA
                     VGAAFTLTGEDGRFRLQQVPAGLAHLRVAAFAFRLDSTDVTVLAGDSVIWNVTLKGGV
                     FYLPGLVVTAGKRPESLENVAASVDVLSDTAIARRAVNTVDEAVNRVPGVQFINGQVN
                     IRGSSGFEQGLGARVLLLVDGVPMNEADRAGIDWDLVPVDDAERIEIVKGAGSSLYGS
                     AALGGVVNIITRDIPEGIHGRVRFFGGGFANPPDSLWKFRNFTGMQGGGSLTGSFGTD
                     QVRGALTVGGRHSDGYRQQDASDYLQVAGRGEWLPDPVTHVRFTGTWATHQYQVPLAW
                     CGPNQCANMRGLAYQPFLVDTADSGNHTRSDHGYLTATVTRTPDENTTWMGRASWLRT
                     HFTDFQKAGDDFAVADRFGAEGRIVTTPSPDQTVTVGGELTDNQVTSDIFGNHSQEQF
                     AAYGEAERVVGKIRLTAGVRADFLADDGAGLSAVISPRVAAVLPSTLGIWRASVGRGF
                     RAASMGEKFVTTPVPPFEVVPNPSLHAETAWTGEIGNTAVITPWLSTNTAIFYTRATD
                     YISPELVGDSIQFQNVQRARLAGVDVSLVASPFTPRLTTSVAYTYLDAWELATDSTPG
                     RPLAFRPRHLVTLSADYAVGPASVGADFRYSSRFERVELYETDSRVAAKTLDLRA"
BASE COUNT          449 a          966 c          924 g          466 t
ORIGIN      
        1 gcgtcgcgca agcgctcgaa caccgtgccg tcagctcttg cggatggttt tggcgaagaa
       61 cgtggaaagc acgggctcga ccgcgcgcga gccacacgag gggcagacgg gccgccgctt
      121 gccgtgctcc gagatggtga gctgttccgt aaacacttcg tcacacgcgg tacagcggta
      181 ctcgtagagg ggcatggccg aaaggtgcgg gtcgtgagag gtccggtcaa gttgactgaa
      241 cgccggaagt ggccagattg cttggagatg cggcgcttcc gattgttggt tctcgctctg
      301 gtgctggggt gcaacgacgg cctccagccg acgaccattt gcggtccgca cttggtgggg
      361 atttgcgggc acatcacgtt ccggggcgcc gtcccggact ccacggacgt gatgtatctc
      421 gtcgcgtacg gaagcttccc ccagacggaa aacgatctct tcaatttcct gcccttcccg
      481 ccgcagacac tccagatccc actcgcggga gactcggtcg tcccctacgg tctcgcgctt
      541 cccaacaacc agtatcactg ggtgctcgcg gtctggaaga aagtcggcac gctgaacccg
      601 tccgacacgg ccagcgccga tgccctcctg cgcgaagccg gttactaccg cgatcccgcc
      661 gatactacaa agcccggcgt cgtcaccgtc cactcggcgg ggaccggcgg cgtcgacttc
      721 gtcgtcgact tcaacaacat gcacagcgtg tcgtacttct tcccgccatg acgtcccggc
      781 tcgcactcgc agcggccctc ctcatcgcca cccccgccgc cgcccaacaa acggggacgg
      841 tcgtcggcac cgttcgcgac acggccggcg caccggtggc cggagcgaag gtcgaagcgg
      901 tcggggccgc cttcacgctg accggggagg atggacgctt tcgcctgcag caggtgccgg
      961 cgggcctggc ccatctccgg gtcgccgcct tcgcgttccg gctcgactcg acggacgtca
     1021 cggtcctcgc cggcgactcg gtgatctgga acgtgacgct caagggcggc gtcttttacc
     1081 tccccggcct cgtcgtgacc gcgggcaagc gtcccgaatc cctcgagaat gtcgcggcgt
     1141 cggtcgatgt cctgtccgac accgccatcg cgcgccgcgc cgtgaacacc gtcgatgagg
     1201 cggtcaaccg cgtgccgggc gtccagttca tcaacggcca ggtcaacatc cgcgggtctt
     1261 ccggattcga acagggactg ggcgcccgcg tgctgctcct ggtcgacggc gttccgatga
     1321 acgaggccga tcgcgccggc atcgactggg acctggtccc ggtggatgac gccgagcgga
     1381 tcgagatcgt gaagggagcg ggctcgtcac tgtacggctc ggcggccctg ggcggcgtgg
     1441 tgaacatcat cacgcgggat attcccgaag gcatccacgg ccgcgtgcgg ttcttcggcg
     1501 gcggcttcgc caatcctccg gattccctgt ggaaattccg gaacttcacc ggcatgcagg
     1561 gtggcggctc gctgacggga tccttcggca ccgatcaggt tcgcggggcc ctgacggtcg
     1621 gcggccggca ctcggatggc taccgccagc aggatgccag cgactacctc caggtcgcgg
     1681 ggcgaggcga gtggctgccc gacccggtga cccacgtgcg ctttaccggc acctgggcga
     1741 cgcaccagta tcaggttccc ctggcgtggt gcggcccgaa tcagtgcgcg aacatgcgtg
     1801 ggctcgccta ccagcccttc ctcgttgata cggccgactc cgggaaccac acccgctcgg
     1861 atcatggcta cctcacggcg acggtcaccc gcacgccgga cgagaacacc acctggatgg
     1921 gacgcgcgtc ctggctgcgg acccatttta cggacttcca gaaggcggga gacgacttcg
     1981 ccgtcgccga ccggttcgga gccgaaggac ggatcgtcac caccccgagt cccgaccaga
     2041 cggtgaccgt gggcggcgag ttgaccgaca accaggtgac gagcgacatc ttcgggaatc
     2101 actcccagga acagttcgcc gcgtacggcg aggccgagcg cgtggtgggc aagatccgcc
     2161 tcacggccgg cgtgcgcgcg gacttcctcg ccgacgacgg cgccgggctg tcggccgtca
     2221 tcagcccgcg ggtcgccgcc gtgctgccat cgacgctggg tatctggcgg gcatcggtcg
     2281 gccgggggtt ccgcgcggca tccatggggg agaagttcgt caccactccg gtgccgccgt
     2341 tcgaagtcgt gccgaatccc agcctgcacg ccgagacggc gtggactgga gaaatcggca
     2401 acaccgcggt catcacaccc tggctctcga ccaacaccgc gatcttttac acgagggcca
     2461 ccgattacat ctcgccggag ctcgtgggag actcgatcca gttccagaac gtccagcggg
     2521 cccggctggc gggggtcgac gtcagccttg tcgcatcccc cttcacaccc cgtctgacca
     2581 cgagcgtggc gtacacgtac ctcgacgcct gggagctggc gaccgattcg actccgggac
     2641 ggccgctggc attccgcccg cggcatctcg tcacgctatc ggccgattac gcggtaggcc
     2701 cggcgtccgt gggcgcggat ttccggtact ccagccggtt cgaacgcgtg gagctgtacg
     2761 agaccgactc gcgggtggcg gccaagaccc tcgacctgcg ggccg
//
LOCUS       DASUBP010000034         4485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_872865, whole genome shotgun sequence.
ACCESSION   DASUBP010000034 DASUBP010000000
VERSION     DASUBP010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4485
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_872865"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2514)
                     /gene="rpoC"
                     /locus_tag="VFU75_01715"
     CDS             complement(<1..2514)
                     /gene="rpoC"
                     /locus_tag="VFU75_01715"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEU5465571.1"
                     /translation="MIDFRSGREPKGSNFDYIQIRIAAPEEIRGPKDSKERERLELQG
                     QKTWWSCGEVTKPETINYRSFKPERDGLFCERIFGPVKDWECHCGKYKRIRYRGVICD
                     RCGVEVTLSKVRRERMGHIELSVPVAHIWFFKTLPSPMGNLLDMTLRDLERVIYYTNY
                     VVIDPGEQEVQERELLDEDRYLELRAKAKSEGDAAFRADIGSPAVRSLLERINVDKLS
                     DELRESVANETSQHRKKQMLKRLKIVDAVRNSGNAGESRNNPGWMILDVIPVLPPDLR
                     PLVPLDGGRFATSDLNDLYRRVINRNNRLQKLILHRAPEVILRNEKRMLQEAVDALFD
                     NGRRSKAIRGRGKRPLKSLSDMLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELRLHQC
                     GLPKAMAVELFKPFIIHKLVEKGIAETVKRAKKIVEKESPEVYEILEEIIQDHPVLLN
                     RAPTLHRLGIQAFEPVLVEGKAIRIHPLVCAAFNADFDGDQMAVHVPLSFESQLEARL
                     LMISSNNVLKPSDGRPVAEPSQDMVLGCYFLTKEPVDFEQARATAPHIGNAAELEMGL
                     ATKRLNFHSPVHFHVDGKWVATTAGRVIFNSILPAALIATLGYQNKVMRKKDLSELVF
                     ESYRRAGLAPTVQFLDSLKEFGFRYATQGGVSIGIADLEIPGEKLPLLHDAEARVERF
                     QRAYATGQITFGERYNKVIDAWTHANNDIAEAMVATMRKSKGGFNPVFMMFDSGSRGS
                     RDQIRQLAGMRGLMAKPQKKLTGGIGEIIESPIKSNFREGLSVLEYFISTHGARKGLA
                     DTALKTADAGYLTRRLVDVAQDVTITEEDCGTILGLEMTA"
     gene            complement(2530..>4485)
                     /locus_tag="VFU75_01720"
     CDS             complement(2530..>4485)
                     /locus_tag="VFU75_01720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU5465572.1"
                     /translation="LVKDDVYSSIHIQELELHVRDTKRGREEITREIPNVAEESLVDL
                     DERGIVRIGAHVKAGDILVGKITPKGETELSPEEKLLTAIFGEKAKDVKDSSLKVPPG
                     MEGVVIDVKIFSRVEDQVVEKDRGERIGEVRRLEADEKARITEAVFGELRELLSGQEV
                     GLMLKNGTVEEFLPAGTKLTRPELAEVDFNDVDLKTLRVGNKSVNEKIRAVLDAATQE
                     RGKIEEKSEDQIDKILQPDELPPGVIQLVKVYLAEKRKISVGDKMAGRHGNKGIIARI
                     VPEEDMPILPDGRPVDIVLNPLGVPSRMNVGQILETHLGWAARILGFEAKTPVFQGGD
                     ETEIGVLLRLAGLAWAADILRLDVKPPELDTDRVRALVADLANLPLGDGARPGLGLAG
                     LALLGNRALTAETRELAHQFTDFLKFAAKELAERELTQHRVEQALHQELMDAEDAGKA
                     EKTALKAGLKDIEKFLEHSKPSEVLEAIGLPALAALLGPKSESDADAAAVELLRVAGS
                     SAAGKCRLRDGRSGEYFESEVTVGTIYMMKLSHLVDDKIHARSIGPYSLVTQQPLAGK
                     AQFGGQRFGEMEVWALEAYGAAHTLQEILTVKSDDVNGRSRVYEAIVKGQNLPKPGIP
                     ESFNVLVKELQALGLKVTLGVTDDVEE"
BASE COUNT          660 a         1563 c         1400 g          862 t
ORIGIN      
        1 cgcggtcatc tcgaggccga ggatggtgcc gcaatcttcc tcggtgatcg tgacgtcctg
       61 cgccacgtcc accagcctcc gggtcaggta gccggcgtcg gccgtcttga gcgccgtatc
      121 cgccagaccc ttccgggcgc cgtgggtcga gatgaagtac tcgagcaccg acagcccttc
      181 acggaagttc gacttgatcg ggctctcgat gatttcgccg ataccgcccg tcagcttctt
      241 ctgcggcttg gccatcaggc cgcgcatgcc cgccagctgc cggatctggt cacgggaacc
      301 gcggcttccg gagtcgaaca tcatgaacac cggattgaag ccgcccttcg acttccgcat
      361 cgtcgcgacc atggcctcgg cgatgtcgtt gttcgcgtgg gtccaggcgt cgatgacctt
      421 gttgtaccgt tcgccgaacg tgatctgtcc ggtggcgtag gcgcgctgga accgctccac
      481 ccgcgcttcc gcgtcgtgga gcaggggcag cttctcgccc ggaatctcga gatccgcgat
      541 cccgatcgag acgccgccct gggtggcgta ccggaagccg aactccttga gcgagtccag
      601 gaactgcacc gtcggggcga gccccgcgcg gcgatagctc tcgaacacca gctcggagag
      661 gtccttcttc cgcatcacct tgttctgata tccgagcgtc gcgatgagcg ccgccggaag
      721 gatgctgttg aaaatgaccc gtcccgcggt ggtcgcaacc cacttgccgt cgacgtggaa
      781 atgcaccggc gagtggaagt tcagccgctt ggtggcgagt cccatctcga gctcggcggc
      841 gttcccgata tgcggggccg tcgcgcgcgc ctgctcgaag tccaccggct ccttggtcag
      901 gaagtagcag cccagcacca tgtcctggct gggctccgcc accggccggc cgtcggacgg
      961 cttcagcacg ttgttggacg aaatcatgag cagccgcgcc tcgagctggc tctcgaacga
     1021 gagcggcacg tgcacggcca tctggtcacc gtcgaagtcc gcgttgaacg ccgcgcagac
     1081 cagcgggtga atccggatcg ccttgccttc caccagcacc ggctcgaacg cctggatgcc
     1141 gaggcggtgg agggtgggcg cgcggttcag gagcaccggg tggtcctgga tgatctcttc
     1201 caggatctcg tagacctccg gactctcctt ctccacgatc ttcttggccc gcttgacggt
     1261 ctcggcgatc cccttctcca ccagcttgtg gatgatgaag ggcttgaaca attcgaccgc
     1321 catcgccttg ggcagaccgc actggtgcag gcggagctcc gggcccacga cgatcaccga
     1381 gcggcccgag tagtccacgc gcttgccgag caggttctgc cggaagcggc cctgcttgcc
     1441 cttcagcatg tccgagagcg acttcagcgg acgcttgccg cggccgcgaa tggccttgga
     1501 gcggcggccg ttgtcgaaca gcgcgtccac cgcctcctgg agcatgcgct tctcgttccg
     1561 gagaatgacc tccggcgccc ggtgcaggat cagcttctgc agccggttgt tccggttgat
     1621 cacgcgccgg tagagatcgt tcagatcgga cgtggcgaag cgcccgccgt cgagcggcac
     1681 cagcggccgc aggtcggggg gcagcaccgg gatgacgtcg aggatcatcc agcccgggtt
     1741 gttgcgcgat tcacccgcgt tgccggaatt ccggacggcg tccacgatct tcagccgctt
     1801 cagcatctgc ttcttgcggt gctggctggt ctcgttggcc acggattccc gcagctcgtc
     1861 cgagagcttg tccacgttga tccgctcgag gagcgaccgc accgcggggg acccgatgtc
     1921 ggcccggaac gcggcgtccc cttcgctctt ggccttggcc cggagctcga ggtagcggtc
     1981 ttcgtccagc agctcgcgct cctggacttc ctgctcgccg gggtcgatca ccacgtagtt
     2041 cgtgtagtag atcacccgct cgaggtcacg cagggtcatg tcgagcaggt tgcccatcgg
     2101 cgacggcagc gtcttgaaga accagatgtg cgccaccggg accgacagct cgatgtggcc
     2161 catgcgctcg cgccggactt tggacagcgt cacttccacg ccgcagcggt cgcagatcac
     2221 gccgcgatag cggatccgct tgtacttgcc gcagtggcac tcccagtcct tcaccggacc
     2281 gaaaatgcgc tcgcagaaga ggccgtcccg ttcgggcttg aacgagcggt agttgatggt
     2341 ttcgggcttg gtcacctcgc cacaggacca ccaggtcttc tggccctgga gctcgagacg
     2401 ctcccgttcc ttcgaatcct tgggcccgcg gatctcctcg ggcgccgcga tccggatctg
     2461 gatatagtca aagttcgagc ccttgggctc gcgtccgctg cggaaatcga tcatcggttc
     2521 tccctcccct tattcttcga cgtcgtcggt gacgcccagg gtcaccttca atcccagggc
     2581 ttgcagttcc ttcaccagca cgttgaagga ctcggggatg ccgggtttcg gcagattctg
     2641 ccccttcacg atcgcttcgt acactcggct gcggccgttc acgtcgtcgg acttgacggt
     2701 cagaatctcc tgcagcgtgt gggccgcgcc gtacgcttcc agcgcccaga cttccatctc
     2761 gccgaaccgc tggccgccga actgcgcctt cccggcgagc ggctgctgcg tcaccagcga
     2821 atacggtccg atggaccggg cgtgaatctt gtcgtccacc aggtgcgaca gcttcatcat
     2881 gtagatcgtg ccgacggtga cctcggactc gaagtactcg cccgagcggc cgtcgcgcag
     2941 gcggcacttg cccgccgccg acgatccggc gacccggagc agctccaccg ccgccgcgtc
     3001 cgcgtcggac tcggacttgg gaccgagcag ggccgcgagg gccggcaacc cgatcgcctc
     3061 gagcacctcg gacggcttcg agtgctccag gaacttctcg atgtccttga gcccggcctt
     3121 cagcgccgtc ttttcggcct tccccgcatc ctcggcatcc atcagctcct gatggagcgc
     3181 ctgctccacc cggtgctgcg tgagctcgcg ctccgccagc tccttcgccg cgaacttgag
     3241 gaagtcggtg aactgatgcg ccagctcgcg cgtctcggcc gtgagcgcgc ggttcccgag
     3301 gagcgcgagt cccgcgaggc caagtcccgg ccgggcccca tcgcccaacg ggaggttggc
     3361 cagatccgcc accagcgccc gcacccggtc ggtgtcgagc tcgggcggct tcacgtccag
     3421 ccgcaggatg tccgccgccc acgccagtcc ggcgagacgg agcagtaccc cgatctccgt
     3481 ttcgtcaccg ccctggaaga cgggggtctt cgcttcgaag cccaggatcc gcgcggccca
     3541 cccgaggtgg gtctcgagga tctgtcccac gttcatccgc gacggcacgc cgagggggtt
     3601 gagcacgatg tccaccggcc gcccgtcggg caggatgggc atgtcttcct cgggcacgat
     3661 ccgggcgatg atgcccttgt tgccgtgccg tcccgccatc ttgtcgccca ccgagatctt
     3721 ccgtttctcg gcgagataga ccttcacgag ctggatcacg cccgggggga gctcgtcggg
     3781 ctggaggatc ttgtcgatct gatcctccga cttctcctcg atcttgcccc gctcctgggt
     3841 cgccgcatcg agcacggcgc ggatcttctc gttgacgctc ttgttgccga cgcggagggt
     3901 cttcaggtcc acgtcgttga agtccacctc ggccagctcg ggccgggtga gcttggtgcc
     3961 ggccggcagg aactcctcca ccgtgccgtt cttcagcatc aggccgactt cctggcccga
     4021 gagcagctca cgcagctcgc cgaagaccgc ctcggtgatc cgggccttct cgtcggcttc
     4081 gagccggcgg acctcgccga tccgctcacc gcggtccttc tccaccacct ggtcctcgac
     4141 gcgcgagaag atcttcacgt cgatgaccac gccttccatc cccggcggca ccttgaggga
     4201 cgaatccttc acgtccttcg ccttttcgcc gaagatcgcc gtcaggagct tctcttccgg
     4261 cgacagctcg gtttcgccct tcggggtgat cttgccgacc aggatgtcgc ccgccttcac
     4321 gtgggcgccg atgcgcacga tgccgcgctc gtccaggtcc accagcgact cttccgcgac
     4381 gttcgggatt tcccgggtga tttcctcacg gccccgcttg gtgtcccgga cgtgcagctc
     4441 gagctcctgg atgtggatcg acgagtacac gtcgtccttc accag
//
LOCUS       DASUBP010000035         7527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1227260, whole genome shotgun sequence.
ACCESSION   DASUBP010000035 DASUBP010000000
VERSION     DASUBP010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7527
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1227260"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VFU75_01725"
     CDS             <1..517
                     /locus_tag="VFU75_01725"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEU5465573.1"
                     /translation="AHLASRTAPTGLWHGHPVAGVVNDPTAAKAGGVSGNAGLFSTGE
                     NLARFVQFLLRGGTRPDGKPLVSPETVKLFTTRASQADVGPDHRALGWQAVPTDEEVS
                     SAGRLFGPRSFGHTGWTGTSIWIDPDRDVFVILLTNRAFAPRARKPFTVLKTVRGHLA
                     DAAARAVDGGR"
     gene            577..885
                     /locus_tag="VFU75_01730"
     CDS             577..885
                     /locus_tag="VFU75_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEU5465574.1"
                     /translation="MTDVQLTEEQLREALRSVKDPELNLNIIDLGLVYGVDPGDGDVR
                     VTMTLTSPGCPAGPQIIHDIYEHLKPIPGVKDVDVDLVWEPYWSPEKIEPKIRAMMGF
                     "
     gene            complement(891..2108)
                     /locus_tag="VFU75_01735"
     CDS             complement(891..2108)
                     /locus_tag="VFU75_01735"
                     /inference="COORDINATES: protein motif:HMM:NF013755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HEU5465575.1"
                     /translation="MPNSTRPSPKRSKRSRREWSSWARRISISDARYVATPEALAQVV
                     GAFRRESPVAVDTEAASFHRFVDRIYLVQLSTRRATAIVDPVAIADLAPVGALLADPD
                     VEKIFHDADYDLRTLDRDYGFRAVRLFDTRVAAQLAGEPAIGLAALLEKYLGLKLSKA
                     HQKADWSQRPLTPGMLEYAAADTAHLPALRDALEARLRELGRWEWATEEFTRIEGLRW
                     TVPADDGEAYLRVKGAKALRPRQLAVMRELYTWREGIARREDKAQFRIIGNESLLGVS
                     RALPRTLDDLPRVKELPASLAKRHGPALLEAVEKGLAVPEKDLPRIVRVPRPPHDPSL
                     DVRQEKLKAARNARAAELGLDPGVLLGRVALEAVARAHPTDRAGLARIGELRRWQLSV
                     VGDALLQATAPAV"
     gene            complement(2023..2199)
                     /locus_tag="VFU75_01740"
     CDS             complement(2023..2199)
                     /locus_tag="VFU75_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465576.1"
                     /translation="MRVSTDYIRHAARAARKCAGDPLGGEPQPPDPEFYKTLAKALEE
                     IAAGMEQLGKADQH"
     gene            2287..3201
                     /locus_tag="VFU75_01745"
     CDS             2287..3201
                     /locus_tag="VFU75_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU5465577.1"
                     /translation="MGTWPTPLEPNPVLAARTGVPELWVKREDRSSALYGGNKVRGLE
                     FLLRDVGPGDVVVTIGGWGSTHCLATACHARALGAQAVLAQFPQGHNDSEARTSRASG
                     SASSRVFAAARWAGFPAAWLRAWNLAGRLGRRVYIPGGGARPVAVLGHMLATLELATQ
                     LPGPPDAIVTALGSGGTAAGILLGCSTLGWPTQVVAVRVAPWVVANRWRVESLSRRAR
                     KLLVARGVQHPQASTSPRLFLVNGMGDGYGVPSAAGEAARAWGAEAGLEADSTYTGKT
                     VAALPDVARMGFTRVVYWHTYAAPGSGE"
     gene            3198..3998
                     /locus_tag="VFU75_01750"
     CDS             3198..3998
                     /locus_tag="VFU75_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5465578.1"
                     /translation="MNRTMRVQVEDAEISVEERGEGPALLLVHGFPFDHDLWRHQLKT
                     FPGWRRIAPDLRGAGTSSVPDDGYSMARYADDLIAVLDAMEVAAAVVCGLSMGGYVIF
                     ELLRRYRGRVRAAILCDTKAEADTEEGKRGREEMMTLARVSGASAVADKMIPALLTPD
                     TRAEHPEVEAQVREMIERAPVNGIVGALKAMRDREDSTAMLAELRVPVLVVTGASDGI
                     STPEGNAAMAGKIKGARFATVSGAAHLPPLEQPFVTTRLIGDFLKSVL"
     gene            complement(4005..4277)
                     /gene="fdxA"
                     /locus_tag="VFU75_01755"
     CDS             complement(4005..4277)
                     /gene="fdxA"
                     /locus_tag="VFU75_01755"
                     /inference="COORDINATES: protein motif:HMM:NF045480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEU5465579.1"
                     /translation="MPYVITQPCIDLKDRSCVDVCPVDCIYEGEKQLFINPDECIDCG
                     ACEPECPVTAIFPEEDVPTQWKEYIALNREIFQSATPPGKPQRKGA"
     gene            4518..4919
                     /locus_tag="VFU75_01760"
     CDS             4518..4919
                     /locus_tag="VFU75_01760"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEU5465580.1"
                     /translation="MGIVKKQRRAETRTLLLEAGLKLISEQGLEFASLDQVANAAGFT
                     KGAIYRQFRSKPAFILAIFEQYAAVARAGAGARQAPWFIPLSLQFAAHAMRDPLLRRR
                     FAVVLAEAPDGNTPDGQLLKALARVLPAPPV"
     gene            complement(4930..>7527)
                     /locus_tag="VFU75_01765"
     CDS             complement(4930..>7527)
                     /locus_tag="VFU75_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU5465581.1"
                     /translation="LGTPAYMAPEQAAADPHVDHRADIYAAGVLAYEMLAGRLPFSAS
                     TPQQMLAAHVTQAPDPLAAHRSTVPPALGALIMRCLSKKPADRPQSAIELVQLLDVIM
                     TPSGGMTPTGSTPVISSGTEAAIKRTHPVRVAALFGVAGVAVLGLVYFLMQRLGLPTW
                     VFAVAGVLLVVGLPIMLVTGVFERKRALAHTTGYVPTNPLAKWFTWRKAILGGGLAFG
                     GLAVVAAIYMTLRLLGIGSVGTLMAKGALKSEDRIILTDFENHSSDSTLGSTLTEAFR
                     VDLSQSRTVHLLDTRQIASALQRMQKPANTPMTGAVAQDLAQREGVKAIVTGQIASVG
                     KSYVLSASILSAADGTVLTAVRENADDEGGLLGALDRLSKDLRERIGESLTTLRATDA
                     LEQVTTSSLDALRKYSEGVRLAMAGDDEGAIPPLRQAVALDTGFAMAWRKLAVELGNT
                     AASTQEQIETATKAYQHRDRLTPSERDQTIGYYSQFADFDIDRAMAAYRDLLTIDSNS
                     IVGLNNLSLLLLVRRDYATAESLLTRGLRNGCGNCYQQLVQLKVVMGQDTAARRLASE
                     YQRAFPRGPNPEGQFFWIASAAHDYAEADRVIAKMRVDFASSPYVQEVVAQTMVATLG
                     ARGRIAEANQVSTEQLMRRADERGLPADYLAASGTQALFDIVLRDRPADGLRKVSEAL
                     ARHPFSSMPAYDRPYTGAAAIYARAGRVDEARQLMAEYARLVPEGVRKANPNRHSALA
                     EIARAEHRYADALGEYWTYHDEVGCAACGFFDVASVFDLMHQQDSALIYYDRFITTPD
                     PFRVWEDEFYLGPAYKRAGELHEAMGDRTQALDYYNKFVDLWKNADAELQPVVRDVRG
                     RIARLAAEH"
BASE COUNT         1196 a         2422 c         2616 g         1293 t
ORIGIN      
        1 ggctcatctg gcgtcacgta ccgcgcccac gggactctgg cacggacatc cggtcgcggg
       61 cgtggtcaac gatcccacgg ccgcgaaggc gggaggggtc tcggggaatg ccgggctgtt
      121 ctcgaccggt gagaacctgg cccgcttcgt ccagttcctg cttcgcgggg gcacgcgccc
      181 ggacggcaag cccctcgtct cgccggagac ggtgaagttg ttcacgacgc gcgcgtccca
      241 ggccgacgtc ggtcccgacc accgcgcgct cgggtggcag gcggtgccca cggacgagga
      301 agtctcgagc gccggaaggc tcttcgggcc gcggagcttc ggtcacacgg gttggaccgg
      361 cacgtccatc tggatcgatc ccgatcgcga tgtgttcgtc attctgctga ccaaccgggc
      421 gttcgcgccg cgggcccgga agcccttcac cgtactgaag acggttcggg gacacctcgc
      481 ggacgccgcg gcccgcgccg ttgatggggg acgctaggaa cttcttgccc gaggacaccg
      541 ttccggggta ggttcgcgag ggcgaggagg agaagaatga cggatgtgca gctcaccgaa
      601 gagcagctgc gagaagcgct gcggtcggtg aaggatcctg agctcaatct caacatcatc
      661 gacctggggt tggtctacgg cgtggacccc ggggacggtg acgtgcgcgt caccatgacg
      721 ctgacctcgc ccgggtgtcc cgcgggtccc cagatcattc acgacatcta cgaacacctg
      781 aagccgattc cgggcgtgaa ggacgtcgac gtggatctgg tgtgggagcc gtattggtcg
      841 cctgagaaga tcgagccgaa aatccgcgcg atgatgggct tctagacggc ctagacggcc
      901 ggagcagtcg cctgcagcag cgcgtcccca accaccgaga gttgccagcg ccggagttcg
      961 ccgatacggg cgagcccggc gcgatccgtc gggtgggccc tcgcgaccgc ctcgagcgcc
     1021 accctgccca gcaggacccc gggatccagt cccaactcgg cggcgcgggc gttgcgggcc
     1081 gccttgagct tttcctgccg tacgtcgagc gacggatcat gtggggggcg tggcacacgc
     1141 acgatccgcg gcaagtcttt ttcagggacg gcgaggccct tctcgaccgc ctcgagcagg
     1201 gccggcccgt ggcgcttcgc gagtgacgcc ggcaattcct tcacacgggg caggtcgtcg
     1261 agggtgcgcg gaagtgcgcg actgaccccc agcagcgatt cgttcccgat gatccggaac
     1321 tgcgccttgt cctcccgccg ggcgatccct tcccgccacg tgtagagctc gcgcatgacg
     1381 gcaagctgcc gcgggcgcag cgccttggcg cctttcaccc ggagataggc ctcgccgtca
     1441 tccgcgggga ccgtccaccg caagccctcg atccgggtga attcctcggt tgcccactcc
     1501 catcggccca gctccctgag gcgcgcttcg agcgcgtcgc gcagcgcggg cagatgcgcc
     1561 gtatcggcgg cggcgtattc gagcatgccc ggcgtcaacg gtcgttgaga ccagtcggcc
     1621 ttctgatggg ctttggagag cttcaggccc aggtacttct cgaggagggc ggcgaggccg
     1681 atcgcaggct caccggccag ctgcgcggcg actcgggtgt cgaagaggcg gacggcacgg
     1741 aagccgtagt cgcgatcgag ggtgcgcagg tcgtagtcgg catcgtggaa aatcttttcg
     1801 acgtccgggt cggcgaggag cgcgccgacc ggcgcgagat ccgcgatagc gacgggatcg
     1861 acgatggcgg tcgcccgccg ggtggacagc tgcaccaggt agatgcggtc cacgaagcgg
     1921 tgaaagctgg cggcctccgt gtccacggcc accggagact cccggcggaa cgccccgacg
     1981 acctgggcca gcgcttccgg agtggcgacg taacgcgcgt cgctaatgct gatccgcctt
     2041 gcccagctgc tccattcccg ccgcgatctc ttcgagcgct ttggcgaggg tcttgtagaa
     2101 ttcgggatca gggggctggg gctcgccgcc cagcgggtcg ccggcgcatt tgcgcgcggc
     2161 ccgcgcggcg tgtctgatgt agtcggtgga tacccgcatg ttggctcact cgcggtgaaa
     2221 ggccttgaag cattggatac cgcggatttc gctcgaaaga tacgcgcggc tttccgtccg
     2281 tgctctttgg gaacctggcc gacgcccctc gaacccaatc ccgtgctggc ggcgcggacc
     2341 ggtgtgcccg agctgtgggt caagcgggaa gaccggtcct ccgccctcta cggtggaaac
     2401 aaggtccggg gtctcgagtt cctgctccgt gacgtaggac ccggagatgt ggtggtcacc
     2461 atcggtgggt ggggatccac ccactgtctc gccaccgctt gccacgcccg cgccctcggc
     2521 gcccaggccg tcctcgccca gtttccgcag ggacacaatg attcggaggc ccggacttcg
     2581 cgtgcgtcgg ggtccgcatc gagtcgagtg tttgcggcgg cgcgctgggc cggtttcccg
     2641 gccgcgtggc ttcgggcgtg gaacctcgcg ggaagactgg ggcgtcgggt gtacatcccg
     2701 ggtggcggcg cccggccggt cgccgtgctc gggcacatgc tcgcgacgct cgagttggcc
     2761 acgcagcttc cggggccacc tgacgcgatc gtcacggcgc tcggctcagg cggaacagcg
     2821 gcgggaatct tgctggggtg ttcgacactc ggctggccga ctcaggtcgt ggccgtgcgc
     2881 gtggccccat gggtcgtggc caaccgatgg agagtggaga gcctgtcacg tcgcgcgcgg
     2941 aagctgctcg tcgcacgggg cgtccagcac ccccaggcct ccacatcccc acgcctcttc
     3001 ctcgtcaacg gcatgggtga cgggtacgga gtaccgtccg cggcagggga ggcggcgcgg
     3061 gcgtggggag cggaggcggg gctggaggcc gattcgacct acaccggcaa gacggttgcg
     3121 gcgctccccg acgtcgcgcg catggggttc acgcgggtgg tgtactggca cacgtacgcg
     3181 gcaccgggga gtggcgagtg aaccggacca tgcgagtgca ggtggaagac gccgagatct
     3241 cggtggagga gcgaggcgaa ggtcccgcgc tgctcctggt gcacggattt ccgttcgatc
     3301 acgacctgtg gcggcatcaa ctcaagacct tccccggatg gcgccggatc gcgcccgatc
     3361 tccggggtgc cggcacgtcg agtgtgccag acgacggata ctcgatggcg cgttacgcgg
     3421 acgacttgat cgccgtactc gatgcgatgg aggtcgcggc cgcggtggtg tgcgggctgt
     3481 ccatgggcgg atatgtgatc ttcgagttgt tgcggcgtta tcggggacgt gtgcgtgccg
     3541 cgattctctg tgatacgaag gcggaggccg atacggagga agggaaacgc gggcgtgagg
     3601 aaatgatgac gctggcccgg gtctctggag cctcggccgt cgccgacaag atgattcccg
     3661 cgttgctcac gcccgatacc agagcggagc atcccgaggt cgaagctcag gtccgggaga
     3721 tgatcgagcg cgccccggtg aacgggatcg tcggggcgct caaggccatg cgggatcgtg
     3781 aggactcgac cgcgatgctc gccgaactgc gggtgccggt gttggtggtg actggagcgt
     3841 cggatggcat cagtacgccg gaggggaacg ccgcgatggc gggaaagatc aagggggcga
     3901 ggttcgccac cgtttccggc gccgcacact tgcccccgct cgaacagccc ttcgtcacga
     3961 cccgcctgat tggcgacttc ctgaaatccg tcctctagcg cccgtcaggc gcctttgcgc
     4021 tgcggcttgc cgggcggagt ggcgctctgg aagatctcgc ggttgagcgc gatgtattcc
     4081 ttccactggg tggggacgtc ttcttctggg aagatggccg tcaccgggca ctcgggttcg
     4141 caggcgccgc agtcgatgca ctcgtcggga ttaatgaaga gctgcttctc gccctcgtag
     4201 atgcagtcca cgggacagac gtccacgcag gaccggtcct tcagatcgat gcagggctgg
     4261 gtaatgacgt acggcatcta ccagtctcct tgagggcttc gtagcgatgc gaaccccaaa
     4321 cgtagaaggg ggtccaagcc agtcaagccc tttgggttgc gcgattctct gcggtgccga
     4381 aattccccga aagcaggata ctcatacgta tctttggtac cgaatggtat cggctcacga
     4441 ctttccttga cgcttcttct cgacgttttc ccttgaggac tttcatattg agatactcga
     4501 tcgtatccta gatacccatg ggaatcgtaa agaagcagcg gcgggctgag acgagaaccc
     4561 ttctgctcga ggccgggctg aagctgatct ctgagcaggg gctcgagttt gcctcgctcg
     4621 atcaggtcgc gaacgccgcc gggttcacca agggcgcgat ctacaggcaa ttccggtcca
     4681 agccggcctt cattctcgcg attttcgagc agtacgccgc tgtcgccaga gccggcgccg
     4741 gagctcgaca ggctccctgg ttcatcccgc tctcgctcca gttcgctgcg cacgccatgc
     4801 gggacccgct cctccggagg cgcttcgccg tggtgttggc ggaggctccg gacggcaata
     4861 ctccggacgg gcagttgctc aaggcgctcg ctcgggtact cccggctccg ccggtgtagc
     4921 cggcgcgccc tagtgctcgg ccgcgagccg cgcgatgcgg ccgcggacat cccgcacgac
     4981 gggttgcagc tcggcgtcgg cgttcttcca gaggtccacg aacttgttgt agtaatcgag
     5041 cgcctgggtg cggtcaccca tggcctcgtg cagctcgccg gcgcgcttgt aggccgggcc
     5101 cagatagaac tcgtcttccc atacccgaaa tggatccggc gtcgtgatga accggtcgta
     5161 atagatcagc gccgagtcct gctggtgcat gaggtcgaag accgaggcca catcgaagaa
     5221 gccgcaagcc gcgcacccga cctcgtcgtg gtaagtccag tactccccca gcgcgtcggc
     5281 gtaccggtgc tcggccctgg cgatctcggc cagggcgcta tgccggttcg ggttcgcttt
     5341 tcggacgccc tccggcacga gtcgagcgta ttccgccatc agctgccggg cctcgtccac
     5401 tcggcccgca cgggcgtaga tagccgccgc gccggtatag ggacggtcgt aggcgggcat
     5461 cgacgagaac ggatgacggg ccagagcctc cgagaccttc cggagtccat cggcgggccg
     5521 atcgcgcagg acgatgtcga acagcgcctg ggttccggac gccgcaaggt agtcggcggg
     5581 caatccccgc tcgtcggccc ggcgcatcaa ctgctcggtg gagacctggt tggcttcggc
     5641 gatccggcct cgcgccccca gcgtcgccac catggtctgg gccacgacct cctgcacgta
     5701 gggactcgac gcgaagtcca cgcgcatctt cgcgatgacc cgatccgctt ccgcgtagtc
     5761 gtgggcagcc gacgcgatcc agaagaattg cccctcggga ttcggcccgc gggggaaggc
     5821 gcgctgatac tcgctcgcca agcgccgcgc cgcggtgtcc tgacccatga cgaccttgag
     5881 ctggaccagc tgctggtagc aattcccgca tccgttccgc agcccgcggg tcagcaggct
     5941 ctcggccgtc gcatagtccc ggcgtacgag gaggaggagc gagagattgt tgaggccgac
     6001 gatgctgttc gagtcgatgg tcaagagatc gcggtacgcg gccatcgcgc ggtcgatatc
     6061 gaagtcggcg aactgcgagt agtaaccaat ggtttgatcc cgctcgctgg gtgtgaggcg
     6121 atcccggtgc tgatacgcct tggtcgccgt ctcgatctgc tcctgagtgg acgccgcggt
     6181 gttgccgagc tcgacggcga gcttgcgcca ggccatggcg aagccggtat ccagggcgac
     6241 ggcctgacgg agtggcggga tcgctccctc gtcgtccccc gccatcgcga gccgcactcc
     6301 ttcggaatac ttccgcaggg cgtcgagcga ggacgtcgtc acctgttcga gggcatccgt
     6361 ggcccggagt gtcgtcaacg actcgccgat gcgctcccgc aggtccttcg agagccggtc
     6421 gagagccccg agaaggcctc cctcatcatc ggcattctcc cgaaccgcgg tgagcacggt
     6481 cccgtctgca gcggagagaa tgctcgccga caacacatag gacttgccca ccgacgcgat
     6541 ctgcccggtg acgatcgcct tcacgccttc gcgctgcgcg agatcctggg cgacagcgcc
     6601 ggtcatcggc gtattcgcgg gcttctgcat gcgctgcagg gcgctggcga tctgtcgggt
     6661 atcgagaaga tgcaccgtgc gggattggga gaggtccacc cggaacgctt cggtcagtgt
     6721 ggagccgagg gtcgagtcgc tggagtggtt ctcgaagtcg gtgaggatga tccggtcctc
     6781 ggacttcaga gctcccttgg ccatcagcgt accgacggac ccgatgccga ggaggcgcag
     6841 ggtcatgtag atcgcggcga ccaccgcgag tccgccgaac gccaggcctc cgcccagaat
     6901 ggctttgcgc cacgtgaacc acttggcgag cgggttggtc gggacgtacc ccgtcgtgtg
     6961 tgccaacgcg cgcttccgct cgaagacccc agtcacgagc atgatcggca ggccgaccac
     7021 caggagcacg ccggcgacgg cgaacaccca ggtcggcagc ccaagccttt gcatcaggaa
     7081 gtagaccagc ccaagaacgg cgacgcccgc cacgccgaac agcgcagcca cgcgcaccgg
     7141 atgcgtgcgt ttgatggctg cttcggtccc cgacgagatc accggagtgc ttcccgtcgg
     7201 cgtcatcccg cccgagggcg tcatgatcac gtcgagcagc tgcaccagct cgatggccga
     7261 ctggggccgg tcggcgggct tcttggagag gcaccgcatg atcaacgcgc ccaaggcggg
     7321 tggcacggtg gaccggtgcg cggcgagcgg gtcgggcgcc tgtgtgacat gtgctgccag
     7381 catctgctgt ggcgtggagg ccgaaaacgg caatctcccc gcgagcatct cgtacgccag
     7441 aacgccggca gcgtagatgt cggcccggtg gtcgacgtgc ggatcagcgg cggcctgctc
     7501 cggtgccatg tacgccggag tgccgag
//
LOCUS       DASUBP010000036         3831 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1213651, whole genome shotgun sequence.
ACCESSION   DASUBP010000036 DASUBP010000000
VERSION     DASUBP010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3831)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3831)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3831
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213651"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1599
                     /locus_tag="VFU75_01770"
     CDS             <1..1599
                     /locus_tag="VFU75_01770"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF003792.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEU5465582.1"
                     /translation="AAASLGRLLVERGLKVTIQKFDPYINVDPGTMSPFQHGEVYVTD
                     DGAETDLDLGHYERFIDRSLSQVNNVTSGRIYLSVITKERRGEYLGSTVQVIPHITDE
                     IKGAIRRLAPDHDVVITEIGGTVGDIESLPFLEAIRQFRQEVGRENTLFIHLTLVPFI
                     AATAELKTKPTQHSVRELMQIGIQPDILICRTERPLTEDLKRKIAQFCNVDFGCVVES
                     PDVASIYEIPIRLMEQGLDKEVCHRLRLDTKEPDMRSWAGMVDKILNPPARVRLAVVG
                     KYTELADSYKSIHEALVHGGIANDVGVKIEWIASDRFTSQEVAGELLAGYDALLVPGG
                     FGVRGVEGMLEAIRWAREHKLPFLGICLGLQTAIIEFARNVAKLENTNSSEFEPECEN
                     PVIALMRTQEGIQEMGGTMRLGAYPCRLRTGSRASQIYGGASEVSERHRHRYEVSNAY
                     RDVLAEYGMRCSGLSPDGSLVEMIELPEHPWFVGCQFHPELKSRPTRPHPLFAGFVGA
                     AVAWHKNHAPAADASHSVLAGLKP"
     gene            1604..2425
                     /gene="kdsA"
                     /locus_tag="VFU75_01775"
     CDS             1604..2425
                     /gene="kdsA"
                     /locus_tag="VFU75_01775"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF003543.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HEU5465583.1"
                     /translation="MAAHFGATDAPFLIAGPCVVESDDLNLRVGEALAQVARRLGLAV
                     IYKASYDKANRSRLEAERGPGIETGLRALERVRKSTGLPVLTDVHETGQVAAAAEVAD
                     VLQIPAFLCRQTDLLLAAGKARKPVNVKKGQWLDADGMRGAVEKLRSGGAEEVAVTER
                     GTFFGYGDLVVDMRNFARLRTACMAPVIFDATHSVQQPGKGVGGSSGGLRHFIPPLLY
                     AAAAAGADGFFIETHPDPDHAPSDGPNMLPLDQLEKTLEVAVDVWRAARGAVRVS"
     gene            2422..2994
                     /locus_tag="VFU75_01780"
     CDS             2422..2994
                     /locus_tag="VFU75_01780"
                     /inference="COORDINATES: protein motif:HMM:TIGR01670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEU5465584.1"
                     /translation="MIDAGLARRITVVGFDVDGVLTDGGVYLGLVADHAAEFKRFHVP
                     DGLGLKMLQWAGLHVVIVSGRVSEATTLRARELGIPDVIQDDGAQKLPALERWLEARR
                     LTLAQCAFVGDDLPDVPVLRRVALPIAVRNACAEVKAVATVVTEAEGGKGAAREAAEL
                     ILKARGTWNERVEAYLTERGDAAERTIPAR"
     gene            2963..>3831
                     /locus_tag="VFU75_01785"
     CDS             2963..>3831
                     /locus_tag="VFU75_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HEU5465585.1"
                     /translation="MQPSERSRLVERGRRVLELEAGALAHSARTLSDSFAAAVELLAQ
                     ARGRVIVSGVGKSGLIARKIAATLTSTGTPASFIHPVDSLHGDLGMAGPNDVAILLSK
                     SGASEDLFGLVGQLGRLGVPIIAITGESDSPLGRAATVRLDGGVDEEACAETLAPTTS
                     TTVALAIGDALAVTLLEVKGFTREDFAALHPGGSLGRTLLLRVADVMVTKDLPVLAGD
                     RPMRECVVLLAERRGTVAVVNGGGTLAGVVTAGDLTRLMEKTDKFLDVKVSDVMTRSP
                     KTVAPAELAGSAVR"
BASE COUNT          615 a         1224 c         1385 g          607 t
ORIGIN      
        1 gcggcggcct ccctggggcg actcctggtc gagcgcggac tcaaggtgac gatccagaag
       61 ttcgatccgt acatcaacgt cgaccccggg accatgtcgc ccttccagca cggcgaggtc
      121 tacgtcacgg acgacggcgc cgagaccgac ctggacctcg gccactatga gcggttcatc
      181 gaccgctcgc tctcgcaggt gaacaacgtc accagcggcc ggatttacct gtccgtcatc
      241 accaaggagc ggcgcggcga atacctcggc tccacggtgc aggtgatccc gcacatcacc
      301 gacgagatca agggcgcgat ccgccggctg gcgcccgacc acgacgtggt gatcaccgag
      361 atcggcggca cggtcggcga catcgagtcg ctgccgttcc tcgaggccat ccggcaattc
      421 cgccaggaag tcgggcgcga gaacacgctc ttcattcacc tgacgctggt cccgttcatc
      481 gccgcgaccg cggagctcaa gaccaagccg acgcaacatt ccgtacggga gctcatgcag
      541 atcggaatcc agcccgacat cctcatctgc cggacggaac gccccctgac cgaagacctg
      601 aaacggaaaa tcgcgcagtt ctgcaacgtg gacttcgggt gcgtggtgga gagccccgat
      661 gtggcgtcca tctatgagat cccgatccgc ctcatggagc aggggctcga caaggaagtc
      721 tgccatcggc tgcgtctcga cacgaaagag ccggatatgc gctcgtgggc ggggatggtg
      781 gacaagatcc tcaacccgcc ggcgcgcgtg cgcctcgcgg tggtgggcaa gtacaccgag
      841 ctggccgaca gctacaagag cattcatgag gcgctggtgc acggcgggat cgcgaacgac
      901 gtcggggtga aaatcgagtg gatcgcctcc gaccggttca ccagccagga agtcgcgggc
      961 gagctgctcg ccggatacga cgcactgctg gtgcccggcg ggttcggcgt gcgcggcgtc
     1021 gaggggatgc tcgaagccat ccgctgggcg cgcgaacaca agctcccgtt cctcggcatc
     1081 tgcctcggcc tgcagaccgc catcatcgaa ttcgctcgga acgtggccaa gctggaaaac
     1141 acgaattcca gcgagttcga gcctgagtgt gagaacccgg tcatcgcgct gatgcggacc
     1201 caggaaggga ttcaggagat gggcggcacc atgcggctgg gggcgtatcc ctgccgtctg
     1261 cgcacgggat cgcgcgcctc tcagatttat ggtggcgcct cggaagtgag cgagcgtcac
     1321 cggcaccggt acgaagtgtc gaacgcgtat cgcgatgtgc tggcggagta cggcatgcgg
     1381 tgttcgggcc tctcgcccga cggctccctg gtcgagatga tcgagctgcc ggaacatccc
     1441 tggttcgtcg ggtgccagtt ccaccccgag ctcaagtccc ggcccacccg cccgcatccg
     1501 ctctttgcgg gcttcgtggg cgccgcggtc gcctggcaca agaaccacgc gccggccgcc
     1561 gacgcgagcc attccgtgct ggcgggactc aagccctaag cgcatggcgg cgcacttcgg
     1621 agcgaccgac gccccgttcc tgatcgcggg gccctgcgtc gtcgagtcgg acgacctgaa
     1681 cctgcgggtg ggcgaagcgc ttgcgcaggt cgcccggcgg ctgggtctcg ccgtcatcta
     1741 caaggcgtca tacgacaagg ccaaccggtc ccggctcgag gccgagcgtg ggcccgggat
     1801 cgagacgggg ctccgcgcgc tcgagcgggt gcggaagtcc acgggactgc ccgtcctcac
     1861 cgacgtgcac gagaccgggc aggtagccgc cgcggcagag gtcgccgacg tcctgcaaat
     1921 cccggccttt ctgtgtcgcc aaaccgatct gctcctcgcc gcgggcaaag cccggaagcc
     1981 ggtgaacgtg aagaagggcc agtggctcga tgcggacggg atgaggggtg cggtcgagaa
     2041 gctgcggtcg gggggcgccg aggaagtggc ggtcaccgag cgaggcacct tcttcggata
     2101 cggggatctc gtcgtcgaca tgcggaactt cgcccgtctc cggaccgcgt gcatggcgcc
     2161 ggtgattttc gacgcgacgc actccgtgca gcagccgggg aagggagtgg gcggttccag
     2221 tgggggcctc cggcacttca tcccgcccct gttgtacgcc gccgcggcgg cgggggccga
     2281 cgggttcttc atcgagacgc acccggatcc cgatcacgcc ccttcggacg ggccgaacat
     2341 gttgcccctg gatcagctgg agaagacgct cgaggtcgcg gtcgacgtgt ggcgcgcggc
     2401 ccgaggggcg gtccgggttt cgtgatcgac gcgggcctgg ccaggcggat cacggtcgtc
     2461 gggttcgacg tggacggcgt gttgacggat ggcggggtct acctcggcct cgtcgccgat
     2521 catgcggccg agttcaagcg gttccacgtt cccgacggtc tgggattgaa aatgctgcaa
     2581 tgggccggcc tgcacgtcgt catcgtcagt ggccgggtct ccgaggcgac cacactgcgt
     2641 gctcgcgagc tcgggattcc ggatgtgatt caggatgacg gcgcgcagaa gttgccggca
     2701 ctggagcgct ggctcgaagc gcggcgcctg accctggccc agtgcgcgtt cgtgggggac
     2761 gatctgcccg atgtgccggt gctgcggcgc gtggcgctcc ccattgccgt acgtaacgcc
     2821 tgtgccgagg tgaaggcggt ggcgaccgtc gtgaccgagg ccgagggggg caagggggcg
     2881 gcgcgggagg cggcggaatt gatcctgaag gcgcgcggga cgtggaacga acgtgtggag
     2941 gcatacttga ccgagcgtgg ggatgcagcc gagcgaacga tcccggctcg ttgaacgggg
     3001 acgccgggtc ctggagctgg aggccggcgc gctcgcgcac tcggcgcgga cgctctcgga
     3061 ctctttcgcg gcggccgtcg agctcctcgc gcaggcgcgg ggccgggtca tcgtctccgg
     3121 ggtagggaag tcggggttga tcgcgcgcaa gatcgcggca actctgacct cgacgggcac
     3181 cccggcgtcg ttcattcacc cggtcgactc gctccacggg gacctcggca tggcaggacc
     3241 gaacgacgtc gccatcctgc tctccaagag tggcgcatcc gaggatctgt tcgggctggt
     3301 cggccagctg gggcgactgg gtgtcccgat catcgcgatc acgggcgaat cggactctcc
     3361 gctcgggcgc gccgcgacgg tgaggctcga cggcggtgtg gacgaggaag cctgcgcgga
     3421 gacgctcgcg ccgaccacca gcaccacggt ggccctggcg atcggcgatg cgctcgcggt
     3481 cacgctgctc gaggtgaagg gcttcacgcg cgaagatttc gcggcgttgc acccgggggg
     3541 atccctgggc cggacgctgc tcctccgcgt ggccgatgtc atggtgacga aggacctccc
     3601 ggtgctcgcg ggggatcgcc cgatgcgcga gtgcgtcgtg cttctcgcgg agcgccgggg
     3661 tacggtcgcc gtggtcaacg ggggggggac cctcgccggt gtcgtcacgg ccggtgatct
     3721 gacccggttg atggagaaga cggacaagtt tctggacgtg aaggtgtcgg acgtgatgac
     3781 gaggtcgccc aagaccgtgg cacccgcgga gctcgcgggg tcggcggtgc g
//
LOCUS       DASUBP010000037         2080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1064028, whole genome shotgun sequence.
ACCESSION   DASUBP010000037 DASUBP010000000
VERSION     DASUBP010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1064028"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="VFU75_01790"
     CDS             complement(<1..569)
                     /locus_tag="VFU75_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465586.1"
                     /translation="MNRRGFMAQAGRLVAGSSLAALFPFVRLGREADASLLIPMDDAQ
                     SDHLKAYGVTYRAIQAGGKVEWFLNYRGGSFLLPDVDAIRRDASLAGVAFTPVDDGTV
                     ANIRGEIAGGNMDTVQLERAPKVAVYVPPFAAPWDDAVTMALQYAGVPFEKVWDPEVL
                     SGKLKQYDWLHLHHEDFTGQYSKFYLLYQG"
     gene            complement(566..>2080)
                     /locus_tag="VFU75_01795"
     CDS             complement(566..>2080)
                     /locus_tag="VFU75_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465587.1"
                     /translation="ELVPLVERAAHATQERDAGVLGLELRTFVALSMNDSVAALAHRW
                     TARVPGDETPWREWAVAKLDQQDAADARRILLEGRKAVGNPRAFAIELAEIAASTGDW
                     AGAAQNWGDAVTTDDGQIPNAVSQLESTPDVARQGVTDALTKPQQTAPARRLGANLLL
                     DWGHGDQAWTVLESTITDSTRETVMALWSFAQRGAQIGTPVAEGARGHALARFADLTP
                     GPMAMRARTDAARALLEAGDKEGARKVVEQIEGQGGDAGVPAGALIDVLIGDGDLEQA
                     ERRFLTEKGLSGDDQARLRLELARAWTARGELDRAEHVLGADSSVDAVAQRGWIALYR
                     GNVIHARDAFQEAGPYAGDRADATRRTEMLALITDVDSVQGTKLGAALLSLARGDSVN
                     AVDGLRAAAAALATAPRGGVLVLAGRVAAALDSAHQVLARDLFQQVVNDSSRAAGAAP
                     AAAELEWARLLEKQNHSDRAIAHLEHLILQYPASAVVPEARRMLDRLKGSVPRS"
BASE COUNT          317 a          752 c          695 g          316 t
ORIGIN      
        1 ccctgataga gaagatagaa cttcgagtac tggccggtga aatcctcgtg atggaggtgc
       61 agccagtcgt actgcttgag cttgccgctg agcacttcgg gatcccacac tttttcgaag
      121 ggaaccccgg cgtactgcag ggccatcgtg accgcatcgt cccagggggc ggcgaagggc
      181 gggacgtaca cggccacctt gggggcacgc tcgagctgca cggtgtccat gttgccgccg
      241 gcgatctctc cgcggatgtt cgccaccgtg ccgtcatcga caggcgtgaa ggccactccc
      301 gcgagcgaag catcccgccg aatcgcatcc acgtcgggca ggagaaacga tcccccgcgg
      361 tagttcagga accactcgac cttccctccc gcctggatgg cccggtaggt gacgccgtac
      421 gccttgaggt gatcgctctg cgcgtcatcc atcgggatca ggagtgaggc atcggcttcg
      481 cgcccaagtc tcacgaatgg aaagagggcc gcaaggctcg agcccgcgac caagcgcccg
      541 gcctgggcca tgaagccgcg acgattcacg accggggaac cgagcccttc agacgatcga
      601 gcatccggcg ggcctccgga accaccgcgc tggccggata ctgaagaatg aggtgctcca
      661 gatgcgcgat ggcgcggtcg gaatggttct gcttctccag caggcgcgcc cactcgagct
      721 cggcggccgc gggggcggcg ccggcggcgc ggctcgagtc attgacgacc tgctgaaaca
      781 gatcccgggc gaggacttga tgcgcggagt ccagagccgc ggcgacccgg ccggcgagca
      841 cgagcacgcc gccccgggga gccgtcgcga gtgccgcggc ggcggcgcgg agcccgtcca
      901 cggcgttcac ggaatcgccg cgagccagcg agagcagcgc ggcacccagc ttggtgcctt
      961 gaacggagtc gacatccgtg atgagcgcga gcatctcagt ccgccgggtc gcgtccgccc
     1021 gatccccggc gtaggggccc gcctcctgga acgcgtcacg ggcgtggatg acgttgccgc
     1081 gatagagcgc gatccagccc cgctgcgcca cggcatccac gctcgagtca gctccaagga
     1141 cgtgctccgc ccggtccagc tctccacgcg ccgtccaggc gcgggccagc tccaggcgca
     1201 accgggcctg gtcgtcaccc gacagtccct tctccgtcag gaaccgccgc tccgcctgct
     1261 ccaggtcgcc atctccgatc agcacgtcga tcagcgcacc ggccggcacc ccggcgtccc
     1321 ctccctgtcc ttcgatctgc tccacgacct tccgcgcccc ttctttgtct cccgcctcga
     1381 gaagggcgcg agcggcgtcg gtacgcgccc gcatggccat cggccccggc gtcagatcgg
     1441 caaaccgggc cagcgcatgt cctcgcgccc cttcggcgac gggcgtgccg atctgcgcgc
     1501 cccgctgggc aaagctccag agcgccatca ccgtttcgcg cgtggagtcg gtgatggtgc
     1561 tctcgaggac cgtccacgcc tggtccccat gcccccagtc gaggagcagg ttggcaccga
     1621 ggcggcgggc gggcgccgtc tgctggggct tggtgagggc gtcggtcacg ccctggcgtg
     1681 ccacgtccgg cgtcgactcg agctgtgaca ccgcgttggg gatctggcca tcgtccgtgg
     1741 tgaccgcgtc gccccaattc tgcgccgctc ccgcccaatc acccgttgac gccgcaatct
     1801 cggcgagctc gatcgcgaag gcgcgcggat tcccgacagc cttccgtcct tccagcagaa
     1861 tgcgtcgcgc gtcagcggca tcctgttgat cgagcttggc gacggcccac tcgcgccagg
     1921 gcgtctcatc gccggggacc cgcgcggtcc agcgatgcgc cagagcggcg acgctgtcgt
     1981 tcatgctgag cgcgacgaag gtccgcagct cgagtcccag gaccccggca tcgcgctcct
     2041 gggtggcgtg cgccgcccgc tcgacgaggg gcacgagctc
//
LOCUS       DASUBP010000038         9696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_219048, whole genome shotgun sequence.
ACCESSION   DASUBP010000038 DASUBP010000000
VERSION     DASUBP010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9696
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_219048"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="VFU75_01800"
     CDS             <1..1312
                     /locus_tag="VFU75_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5465588.1"
                     /translation="TSDGSLPVNNHYRNGAVNGRLVVRPGDSTEAALSVRYDDGLYFF
                     PTTYPGAPLSNNQHDLDRGPSVGLDLSHGFNALSVHLLGTWHRDNLQYAIGPNDSTDT
                     FNLPFSSNDWITRQSLEGRAEVRLSGSDALTAGSSFEHQRMEGTTLGQVRTRDDGAVF
                     AELVTGLGRPLGVTLGARMEDNQRFGTFGTYRAGVSYRLSGSTRVLGSLGSAFKEPSL
                     FQNFAQGFITGNENLKPEHSFSWEGGVEQDLGSGVAVRATYFDQHFRDQIAYAPGSVP
                     NYQNINGSTARGVELSLHAPLGAWGALSAGYTYLATNVVKGDTTPGSEFQTGHPLIRR
                     PARSATASLSAALPFGGTAGVAVSYVGRRDDIDFNTFPSQRVALAPYGRLDLSAQVPV
                     RLVPGLSIDTRVDNALNAGYDEVASFRARGRTIVFGGSWTVGSH"
     gene            1349..2320
                     /locus_tag="VFU75_01805"
     CDS             1349..2320
                     /locus_tag="VFU75_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465589.1"
                     /translation="MVLLVAAVACSDALEQATPVGQEIVILDAQASSLTLVNAGDFSA
                     ASLALAFPVTGVPQIAGRGSTVLIPGGAHDSLQILTLGGSAKVAAVRNPVGAAFQNDT
                     LAWVADSAFDSLAQVNLRAGQQVLTRLLSGPAVGVAVTDSQVFTVHSTWLTSVNMAGP
                     PVPDSIPLSGVNAHFVVIGDDSLLYVVSRGDSGKANGKLSIVDPVGHVEMVVINGLGE
                     RPGPAVFHPAGRLLIASPTNGILEVNTLTRSMTRGPSDAIKPGGKGVLALSLDARGRL
                     YAVSNTACGSGPGTLYVLSPPPEYRVVTTLPLGTCPVASAVAEMPAQ"
     gene            2329..3099
                     /gene="recO"
                     /locus_tag="VFU75_01810"
     CDS             2329..3099
                     /gene="recO"
                     /locus_tag="VFU75_01810"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEU5465590.1"
                     /translation="MPLVTTPAIVLQTYRYSETSKVVRLATRDLGVQSAIAKGVMRPK
                     SKFGAGLEILGEGTAQLYYRETRELQTLGEFELEVLRGELARDVARFAGATALAEVML
                     KSAPPAALPAAYEALVAGLDALVAASACDVDAAAIRALWHLLGVLGFEPALTNCVRDG
                     TPLDPDPTTPVPFSSAEGGAFCPACAPARAPTQLPPQAYRDLVALGSEGGPLPSLDAA
                     HAAAHRRLVVRFARHHLSEPNKLPALDFWEHHRWSVTS"
     gene            3081..4970
                     /gene="selB"
                     /locus_tag="VFU75_01815"
     CDS             3081..4970
                     /gene="selB"
                     /locus_tag="VFU75_01815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HEU5465591.1"
                     /translation="MVRHVVVGTAGHIDHGKTALVKALTGVDTDRWAEEQRRGITIDL
                     GFAPLPLDQETQVSIVDVPGHEGFVRNMLAGATGIDAALLIIAADEGIMPQTEEHLAI
                     VELLRVRRGVPVLTKRDLVDDEWLQLLRAEVATRLAKSPIRWEPPVATSARTGTGLPE
                     LREAIARVARDVADRVERDLFRLPVDRVFTLKGAGTVVTGSTWSGTVQEGDTVRLLPL
                     DREVRVRSIESHGSTQAQAVPGRRTALALAGVEHQELARGHVVVTGSGWRPTNRVDAG
                     LELLPSAARPLTARTRVRVHLGTAEVLARVTPVEPVAPGGRGVVRLAFEGPLVARGGD
                     RFVIRSYSPVATIGGGSVIDPYPPDAPRRLRQRRLPFEASPGERLAAWVKEARLSGLA
                     KTDLPVRLGITPDAVAEVIASAGKNVLETGDRLVFRSAASREAERLGGVLDAHHASHP
                     LDAGMSLQALRSSVGNGVATGEAVTDQVIDLALRKGILEIDGSLARRPGWRAAVAARA
                     DGDAGRISAHLAAVEWRIPTVSELESEFAGLPVRALLAHLVRGGGVVQVDQERFASPE
                     ALERFQRTLEGVIREVGPVTPAVLRDRLGLTRKYLIPLLEWADRRGVTRREGDTRVLA
                     RLTPG"
     gene            5174..5524
                     /locus_tag="VFU75_01820"
     CDS             5174..5524
                     /locus_tag="VFU75_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465592.1"
                     /translation="MSKPPVELRQNYPNPFNPATTIPFTLGGNLFANGHRPKVSLKIY
                     NVLAQLVAIPILQGTGQRLDEVQVDCANPQGCSFTAYWDGNVRTGQQASSGVYIYQLV
                     VDGQRYTKKMLIMK"
     gene            complement(5582..7543)
                     /locus_tag="VFU75_01825"
     CDS             complement(5582..7543)
                     /locus_tag="VFU75_01825"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing transglutaminase
                     family protein"
                     /protein_id="HEU5465593.1"
                     /translation="MVRVFALLALGILASAPLAAQAPHITPAGDPSVKDDSIYRLAVN
                     PVDHKGEDYVLLLDDGVITYNKDGTGSRTYRQVVQVFTREAAEQFGEQTFDYDSSRER
                     LTINWLRVLSADGKLISDKPVHEQEGIAPVAQEAPVFSDVREHRVSLGGVAPNTIVDY
                     SYTIQTISPIMPGDFRNSWRVTTGRLVRRSRYLVDLPADLQPTIKERNLTFKREVTRA
                     NGRAVYLWATAEVQPWDGEPFAADSNGVVQTIEIAAPLRWDDIAKWFAGLSKDRFVTT
                     PAVVAAFREQLAGARTQDDSLRAAARWVAQDFRYVSLSLGIGGYQPRYPADVLTTKYG
                     DCKDKATMFVALARQMGLKAFPVLLSSVGGVDRTVPTVDAFDHMIAAVERPWGGYLFV
                     DLTSDLTPYGSLPPAEQGEFGLLVHPDGRAEQVTFPKDSIGANRDDDRLVGELLPDGT
                     MQGLYSELQTGTRQYGLRGAFTNELDPNDRSRLAQRIASEIVPDAVPDSLQIFDGRDW
                     NARPYVQLTFHGGKPAELAGETDILTLPLHDYATPKLVTDLAGRPARRFPIDFWGRDG
                     APHEESSSLEMTLPAGWTAQLPPNVDASSIFGRYTAEYRQDGRVLRITRRLVGAPGIQ
                     APDKVGVLVDWLKRIGTDDVKYVVLNRGH"
     gene            7678..>9696
                     /locus_tag="VFU75_01830"
     CDS             7678..>9696
                     /locus_tag="VFU75_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5465594.1"
                     /translation="MIRPERLTVKAAEALQQAAADARSRGNPVLNDAHLFRALLAQDE
                     GVVAPLLQKAGVNVAQLTAETEREIGRFPKQSGEGVEPTVGRELSQAFDRAEKDAKDL
                     GDAYISTEHLLLALAEEKGTTARQLLSAAGVDRKELLAALEAVRGSHRVTDQEPEQKY
                     QALERFTRDLTEVAQKGKLDPVIGRDEEIRRVIQVLSRRTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVHGDVPESLKRKRLLALDIGQLIAGTKYRGEFEERLKAVLKEITGSEGQFI
                     VFIDELHTLVGAGGAEGAVDASNMLKPALARGELHVVGATTLDEYRKYIEKDAALERR
                     FQPVFVGEPSVEDTIAILRGLKERYEVHHGVRITDNALVAAATLSHRYIGDRFLPDKA
                     IDLVDEAASRLRIEIDSLPQEIDEVERKIVQLEIERAALAKEKDAASKERRKSIDGEL
                     SELRERSMGMKAQWQAEKEAIGGLQAQKAQLEKLRAEADQATRRGELQKAAEISYGTI
                     PALEKEIATSERRLGEIQKKAQYLKEEVDAEDIAEIVAKWTGIPVTRMLESEKVRLTK
                     LEEELGRRVVGQPEALAAVANAVRRARAGLQDPHRPTGSFIFLGPTGVGKTETARALA
                     DFLFDDERAMVRLDMSEYMEKHAVARMIGAPPGYVGYEEGGQLTEAIRR"
BASE COUNT         1634 a         3114 c         3335 g         1613 t
ORIGIN      
        1 cacgtcggac ggatcgctcc cggtgaacaa tcattaccgg aatggcgcag tgaacggccg
       61 gctggtcgtc cggcccgggg atagcacgga ggcagccctg tcggtccgat atgacgacgg
      121 tctgtacttc tttccgacga cctatccggg agcgcccctg agcaacaatc agcatgacct
      181 cgatcgtggt ccgtcggtcg gactcgatct cagtcatggg ttcaacgcac tgagcgtcca
      241 cctgctcggt acctggcatc gcgacaatct gcagtacgct atcggcccca atgattcgac
      301 cgacactttc aatctcccat tttcgagcaa tgactggatc acacgccaga gtctcgaagg
      361 gcgtgccgag gttcgcctct cgggttcgga cgccctcacc gctggttcct cgttcgagca
      421 tcaacggatg gagggtacga cgctgggcca ggtgcggacc cgggacgacg gcgctgtctt
      481 cgcagagctg gtcacggggc tcggccggcc cctcggtgtc accctcggtg cccggatgga
      541 agacaaccag cggttcggca ccttcggcac ctatcgcgcg ggcgtatcgt accgcctgag
      601 cgggtccaca cgggtcctgg ggtcgttggg gagcgcgttc aaggagccca gcctcttcca
      661 gaacttcgcg caggggttca tcacggggaa tgagaacctc aagcccgagc actcgttcag
      721 ctgggaggga ggtgtcgagc aggacctcgg ctccggagtg gcggtacggg cgacgtactt
      781 cgaccagcac ttccgcgacc agatcgcgta tgccccaggt agtgtgccga actaccagaa
      841 catcaatggc tcgactgccc gcggagtcga gctctccctc cacgcgccac tgggggcgtg
      901 gggagcgctg tccgccgggt acacctatct ggccaccaac gtggtcaaag gggacacgac
      961 ccccggcagc gagttccaga ccggacaccc gctcattcga cggccggcgc gctccgcaac
     1021 ggcgagcctc agcgcggctc tgccctttgg tgggaccgcc ggcgtggcag tgagctacgt
     1081 gggccgccgg gacgacatcg acttcaatac cttcccctcg cagcgtgtcg cgctggcccc
     1141 ctatgggcgc ctggatctct cggcgcaagt tccagttcgg ctcgtgccag ggctgtccat
     1201 cgacacccgc gtcgacaatg cgctcaatgc cgggtacgat gaagtggcga gtttccgcgc
     1261 acgtggtcgc accatagttt tcgggggttc ctggaccgtc ggatcacact gacgaagggc
     1321 ggcgatgggc ggagtccggt tcgctggagt ggtgctcttg gtggccgcgg tggcctgctc
     1381 ggacgcgctg gagcaggcca cgccggtcgg gcaggagatt gtcatcctgg atgcgcaggc
     1441 gagctccctc acactcgtga atgcggggga cttttccgcg gcgtcgctgg cgctggcgtt
     1501 tccggtgacg ggcgtgcccc agattgcggg tcgcggatcg acggtcctca tcccgggggg
     1561 agcgcacgac tcgctccaga tcctcacgct gggcggctcc gcgaaggtgg cagcggtaag
     1621 aaatcccgtc ggagccgctt tccagaacga cacgctggcc tgggtggccg attccgcatt
     1681 cgattcgctg gcacaggtga acctgcgggc cggtcagcaa gtgctgacgc gtctcctcag
     1741 tggcccggcc gtcggcgtcg ccgtcaccga cagtcaggtg ttcacggtcc acagcacctg
     1801 gctgacgtcg gtcaacatgg ctggcccacc ggttcccgac tcgatcccgc tctccggcgt
     1861 caatgcccac ttcgtcgtga tcggtgacga ctcgctgctc tacgtggttt cgcgcggcga
     1921 cagcgggaag gcgaacggca agttgtcgat cgtggatccc gtcgggcatg tggagatggt
     1981 ggtcatcaac ggactgggtg agcgaccggg gcccgccgta tttcatccgg ccggacggct
     2041 gctcattgca tcgcccacga atggaattct cgaggtgaac actctgacgc ggtcgatgac
     2101 gcgggggccc agtgacgcga tcaagcccgg tggcaaaggg gtcctggcct tgtctctcga
     2161 cgcgcgcggc cggctgtacg ccgtgtcgaa tacggcgtgt gggagcggcc cgggcacctt
     2221 gtacgtgctg tcgcctcccc ccgagtaccg cgtagtcacg acccttcccc tcgggacgtg
     2281 tccggtcgcc tcggccgtgg ccgagatgcc ggcgcagtaa cttccgggat gcccctggtc
     2341 accacgccgg cgatcgtcct ccagacctac cggtacagcg aaacctcgaa agtcgtccgg
     2401 cttgccacgc gcgatctggg ggtgcaaagc gcgattgcga agggcgtgat gcgccccaag
     2461 agcaagttcg gtgcggggct cgagatcctg ggggagggca ccgcccagct ctattaccgg
     2521 gagacgcgcg agctccagac cctcggtgag ttcgagctcg aagtgctgcg cggcgagctg
     2581 gcgcgcgacg tcgcccggtt cgccggtgcg accgcgctcg ccgaggtgat gctcaagtcc
     2641 gcgccccccg cggcgctgcc ggcggcgtat gaggcgctcg tggccgggct cgacgccctc
     2701 gtagcggcgt ccgcctgcga cgttgacgcg gccgcgattc gcgccctctg gcacctgctc
     2761 ggcgtgctgg gattcgagcc ggccctgacc aactgcgttc gggacggcac gcccctcgat
     2821 ccggatccca ccactccggt gccgttctcc tccgccgagg ggggcgcatt ttgtccggcg
     2881 tgcgcgccgg cacgcgcccc aacccaactg cctccgcaag cgtaccggga tctggtggct
     2941 ctggggtccg agggggggcc actcccgtcg ctggacgccg cccacgcggc agcgcaccgc
     3001 cggctggtgg tccgtttcgc ccgtcaccac ttgagcgaac ccaacaagtt gcccgccctc
     3061 gacttctggg agcaccatcg gtggtccgtc acgtcgtagt cggcaccgcc gggcacatcg
     3121 atcatggcaa gacggcgctg gtgaaagcac tcacgggcgt cgataccgac cgctgggccg
     3181 aggaacagcg gcggggcatc accatcgacc tcggcttcgc tccacttcca ctcgatcaag
     3241 aaacgcaggt cagcatcgtg gatgtgcccg ggcacgaagg gttcgtccgg aacatgctgg
     3301 cgggcgcgac gggcatcgat gccgccctgc tgatcatcgc ggcggacgaa ggcatcatgc
     3361 cgcagaccga ggagcacctc gccatcgtcg agctcctgcg ggtccggcgc ggagttccgg
     3421 tactcaccaa gcgcgatctg gtggacgacg agtggttgca actcctccgt gcggaggtgg
     3481 cgacccgcct tgccaagagc cccattcgtt gggagccgcc cgtggcgacc tcggcgcgga
     3541 ctggcacggg gctgcccgag ctccgtgagg ccatcgcacg ggttgccagg gatgtggcgg
     3601 accgtgtgga acgtgacctc tttcggctcc ccgtagaccg cgtgttcacg ctcaaggggg
     3661 cggggacggt cgtcacggga tcgacctgga gcggtacggt ccaggagggc gataccgttc
     3721 gcctcctccc cctcgatcgt gaggtacgcg tccgctccat cgagtcccac ggcagcacgc
     3781 aggcgcaggc cgtccccggc cgacggacgg cgctcgcatt ggccggggtg gagcaccagg
     3841 agctcgcccg ggggcacgtg gtggtgacgg ggagcggatg gcgcccgacg aaccgcgtcg
     3901 acgcagggtt ggagctcctt ccctccgccg cgcgaccgct tacggcgcgc actcgcgttc
     3961 gcgtgcattt gggcaccgcg gaagtgctgg cgcgggtcac accggtggaa ccggtcgcgc
     4021 cgggaggccg gggagtcgtc cgcctcgcgt tcgagggacc actggtcgca cggggtgggg
     4081 atcgctttgt gatccgttcc tactccccgg tcgcgacgat cggcggagga tcggtcatcg
     4141 acccgtatcc acccgacgcg ccgcgacgtc tgcggcaacg ccgcctgccg ttcgaagcaa
     4201 gtccgggtga acgattggcg gcgtgggtga aggaggcccg cctgtccggt ctcgccaaga
     4261 ccgacctccc ggtccggctc ggcatcactc ctgatgcggt cgcggaagtc atcgcgtcgg
     4321 cggggaagaa cgtactggag acgggtgacc ggctggtatt tcggtccgcc gcatcccggg
     4381 aagcggagcg gctcggcggc gtgttggacg cgcatcacgc gtcgcacccc ctcgacgccg
     4441 ggatgtctct gcaggcgttg cgctcgagcg tggggaatgg cgtggcgacc ggcgaagccg
     4501 tgaccgacca ggtcatcgat ctcgcccttc gtaaagggat tctcgagatc gatgggagtc
     4561 tggcaaggag gccggggtgg cgggctgccg tggctgcgag ggcagacggc gacgccggtc
     4621 gcatttcggc gcatctggct gcagttgagt ggcgcatccc gaccgtgtcg gagttagaat
     4681 cagagttcgc ggggctcccg gtgcgcgctt tgctggcgca cctcgtgcgt gggggtgggg
     4741 tagtacaggt ggatcaggaa cggtttgcgt ctccggaagc gctcgagcgc ttccagcgga
     4801 cgttggaggg ggtgatccgg gaggtggggc cggtgacacc ggcggtactt cgggaccggc
     4861 tcgggctgac ccgcaagtac ttgataccgc tgctcgaatg ggcggaccgg cggggggtta
     4921 cccgtcgaga aggggacacc cgggttctgg cccgcttgac acccggctga aacgtcctct
     4981 aagttcttac gcagctgtac attgaagcgg tccggtggag cgattccgtc atccaaagga
     5041 gtagggtcta acgcgccttt ctcgttcgat aggagttttg atgaagcggt atgcctgggc
     5101 agtgatgggg ctcgggctgt gtaccatcgc agcggggagt gctcgggcgc aggcaccagt
     5161 cccgagccag ggcatgtcaa aaccccccgt ggagttgcga cagaattacc cgaacccgtt
     5221 caacccggcg accacgattc cctttacgct gggcgggaat ctgttcgcaa atggtcaccg
     5281 gccaaaagtc tcgctgaaga tctacaacgt cctcgctcag ctggtcgcga tcccgattct
     5341 ccaggggacc ggccagcggc tcgacgaagt gcaggtggat tgcgcgaacc ctcaggggtg
     5401 ctcattcacc gcgtactggg acgggaacgt caggaccggc caacaggcct cgtccggtgt
     5461 ctacatctat cagctggtgg tggatggaca gcggtacacc aagaagatgc tgatcatgaa
     5521 gtgagtgcgc tttgcgaatt caagccggtg cggcccctcg ggaccgcacc ggcttctgct
     5581 tttagtgccc ccggttcaag accacgtact tcacgtcgtc agtgccgatg cgcttcagcc
     5641 aatccacgag cacgccgacc ttgtcgggtg cctggatgcc cggcgcgccc accagccgcc
     5701 gcgtgatccg gagcacccgt ccatcctgtc ggtactcggc ggtgtagcgg ccgaagatgc
     5761 tggaggcatc cacgttcggc gggagctgcg ccgtccaccc ggcagggagc gtcatctcga
     5821 gcgaggatga ttcctcgtgg ggcgcgccat cccggcccca gaagtcgatg gggaagcgcc
     5881 gtgccggacg tcccgcgaga tccgtgacga gcttcggggt cgcgtaatcg tgcagcggaa
     5941 gcgtgagaat atccgtctcc cccgccaact ccgccggctt tccaccgtgg aaggtcagct
     6001 gcacgtacgg ccgcgcattc caatcccgtc catcgaagat ctggagcgag tcgggaacgg
     6061 catcaggaac gatctcactc gcgatgcgct gcgccaggcg tgaccggtcg ttgggatcca
     6121 gctcgttggt gaaggcaccg cgcagtccgt actgacgggt cccggtctgg agctccgagt
     6181 acaggccctg catggtcccg tcgggaagca actcaccgac cagccggtcg tcatcgcggt
     6241 tcgcgccgat cgagtccttc gggaacgtga cctgttccgc gcgtccatcc ggatgcacca
     6301 gcagtccgaa ctcgccctgt tcggcgggcg gcagggaacc gtacggagtg aggtcgctcg
     6361 tcaggtcgac gaagagatag ccgccccagg gacgctcgac cgcggcgatc atgtgatcga
     6421 acgcgtcgac cgtcgggacg gtccgatcca cgccgcccac gctcgacagc aggaccggga
     6481 atgccttgag tcccatctgc cgggcgagcg cgacgaacat cgtcgccttg tccttgcagt
     6541 caccgtactt ggtggtgagg acgtcggcgg gatagcgggg ctgatagccg ccgatcccga
     6601 gggagagcga gacgtaccgg aagtcctggg ccacccagcg ggccgcggcg cgcagtgaat
     6661 cgtcctgcgt cctggccccc gcgagctgct cacggaacgc cgccaccacc gcgggcgtgg
     6721 tcacgaaccg gtccttcgag agaccggcaa accacttggc gatatcgtcc caacgcagag
     6781 gcgccgcgat ttcgatggtc tgcaccacgc cattcgaatc ggcggcgaaa ggctcgccgt
     6841 cccacggttg cacctcggcg gtcgcccaca gatataccgc gcgtccgttg gcgcgggtga
     6901 cctcgcgctt gaacgtcaga ttccgctctt tgatggtcgg ctggagatcc gccggcaggt
     6961 cgacgaggta ccgcgagcgg cggaccagcc ggcccgtggt gacccgccag gagttccgga
     7021 agtccccggg catgatgggg gagatcgtct gaatggtata gctgtaatcg acgatggtgt
     7081 tgggcgcgac gccgccgagg gagacgcggt gctcccggac gtcggagaat acgggtgctt
     7141 cctgcgccac cggggcgatc ccttcctgct catgcaccgg cttgtcgctg atcagctttc
     7201 cgtcagccga aagcacgcgg agccaattga tggtgagtcg ctcgcgactc gagtcgtaat
     7261 cgaaggtctg ctcgccgaac tgctccgccg cctcgcgcgt gaagacctgc accacctgac
     7321 ggtacgtccg ggaccccgtg ccgtccttgt tgtacgtgat cacgccatcg tccaggagca
     7381 ggacatagtc ctcgcccttg tgatccaccg gattgaccgc cagccggtag atcgagtcat
     7441 ccttgacgga tggatccccg gcgggggtga tgtgcggggc ctgagccgcc aagggggcgg
     7501 aggccagaat tcccagggcc aacagggcga aaacacgaac cacaaaacga ctccaaaagg
     7561 gatggtgtga attatacgcc gaagactcga aaggcattca tcaggtcctt ttgacttaag
     7621 ccctgcccat ctgacacctt tcttccggta tggaggttgc cttaaaccgg gaccgacatg
     7681 attcgacctg aacgtttgac cgtaaaggcc gccgaggccc tccagcaggc tgccgccgac
     7741 gcccgcagcc ggggcaatcc ggtattgaac gatgcccacc tcttccgggc gctcctggcg
     7801 caggatgagg gggtcgtcgc gccgttgttg cagaaggccg gggtgaacgt cgcccagctg
     7861 actgccgaga ccgagcgcga gatcggtcgc tttccgaaac agtcggggga gggcgtcgag
     7921 ccgacggtag gccgggagct ctctcaggcg ttcgatcgcg ccgagaagga tgcgaaggac
     7981 ttgggggatg cctacatctc caccgagcac ctgctcctgg cgctggccga ggagaagggg
     8041 accaccgccc ggcagctgct ttcggccgcc ggggtggacc ggaaggagct gctggccgcg
     8101 ctcgaggccg tgcgcgggag ccaccgcgtc accgaccagg agccggagca gaagtaccag
     8161 gcgctggaac gcttcacccg cgacctcacc gaggtcgccc agaaggggaa gctcgacccg
     8221 gtcatcgggc gcgacgagga aattcgccgc gtcatccagg tgctctcccg gcgcacgaag
     8281 aacaaccccg tgctcatcgg cgagcctggc gtgggcaaga ccgccatcgt cgaagggttg
     8341 gcccagcgga tcgtgcacgg ggacgtgccc gagtcgctca aacgcaagcg tctgctcgcg
     8401 ctcgacatcg ggcagctgat cgcgggcacc aagtatcgag gcgaattcga ggagcgtctc
     8461 aaagcggtcc tcaaggagat caccgggtcc gaggggcagt ttatcgtctt catcgacgag
     8521 ctgcacaccc tggtgggggc ggggggcgcc gagggcgccg tcgacgcgtc caacatgctg
     8581 aagcccgcgc tggcccgggg cgagctgcac gtcgtcggcg ccaccacgct ggacgagtac
     8641 cgcaagtaca tcgagaagga cgccgcactc gagcgccggt tccagcccgt attcgtgggc
     8701 gagccgtccg tggaggacac gatcgcgatc ctgcggggcc tgaaggagcg ctacgaggtg
     8761 caccacggcg tccggatcac ggacaacgct ctggtcgccg cggcgactct ctcgcaccgc
     8821 tacatcggtg accgcttcct gccggacaag gccatcgacc tggtggacga ggccgcgagc
     8881 cgtctgcgga tcgaaattga ttcgctgccg caggaaatcg acgaggtgga acggaagatc
     8941 gttcagctcg agattgagcg ggcggcgctt gccaaagaga aggatgccgc ctcgaaggaa
     9001 cggcggaaat cgattgacgg ggagctttcg gagctgcggg agcgatcgat gggcatgaag
     9061 gcccagtggc aggcggagaa agaggccatc ggcggtctgc aggcccagaa ggcccagctc
     9121 gagaaactgc gtgccgaggc cgaccaagcc actcggcggg gcgagctgca gaaggcagcc
     9181 gagatcagct atggaacgat tcccgctctt gagaaggaga ttgcgacctc cgagaggcgt
     9241 ctgggcgaaa tccagaagaa ggcgcagtac ctcaaggaag aggtggacgc cgaggatatc
     9301 gccgagatcg tggcgaaatg gacgggcatt ccggtaacgc ggatgctcga gtccgagaag
     9361 gtgcgtctga cgaagctcga agaagagctg ggccggcggg tcgtgggcca acccgaggcg
     9421 ctggccgcgg tcgcgaacgc ggtgcgccgg gctcgcgccg gcctccagga cccccatcgc
     9481 cccaccggtt ccttcatctt cctcggcccc acgggcgtgg ggaagacgga gaccgcgcgc
     9541 gcgctcgccg actttctgtt cgacgacgaa cgggcgatgg tccgtctcga catgtcggag
     9601 tacatggaga agcacgccgt ggcccggatg atcggcgcgc ccccgggcta tgtcggctac
     9661 gaagagggtg gccagctcac ggaggccatc cgccgg
//
LOCUS       DASUBP010000039         2584 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1104693, whole genome shotgun sequence.
ACCESSION   DASUBP010000039 DASUBP010000000
VERSION     DASUBP010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2584)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2584)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2584
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104693"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2132)
                     /locus_tag="VFU75_01835"
     CDS             complement(<1..2132)
                     /locus_tag="VFU75_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU5465595.1"
                     /translation="MPSRDTIAARLSQGLAGRYRVERELGRGGMAIVYLALDEKHSRP
                     VAIKVLRPEISGAIGAARFLREIQIAAQLNHPHILPLYDSGESNDLLYYAMPYVTGES
                     LRQRLDREPQMSLHDTVQIGGEVASALSYAHARDIVHRDIKPENILLSEGYALVADFG
                     IARAITAAEASGPVTTAGITVGTPVYMSPEQALAEAVDGRSDQYSLGCVLYEMLTGRP
                     PFGGTTAQAVLAKHTLDPVPSIRAVRADVPDALEQVVTLSLAKQPADRYPNAEALDKA
                     LRGSITGEVVTRRVARAEPPHRRRGRRLAMLATFTVIAAAAATTMVRRPHRPAAATIT
                     PASVAVIPFANASGAGRPDYYVDGFTEDLIGALRRIPGLQVAPATSSYRFARTARTPQ
                     ELGQRLGASYLIGGSVLRQGSRVKVQSYLIRAATGDTILPATYDTLDRGNLDVQAEIV
                     RSITSQLRVNLSAATAASLARRPTRNPAAYDLYLQGRSALEQRTQAALLRSIQLFHAA
                     IHADTNFALAYAGLADAYGFMAAFQYGPPRDYFPQSRAAGNEAVARDSLLPEAYASLG
                     FVALFYDWNVPDALRQLDEALRLDSTLTRAHLFRSHVFITQHQSDSAVAEMRAAQALE
                     PFSLIVGTRLMSALYFAGREEEAAREGELVLAGDSTYALAVPDLARSYLMLGRCDDAI
                     RITSRPMTNFYEGVRTLAGIAYAKCGQP"
     gene            complement(2203..>2584)
                     /locus_tag="VFU75_01840"
     CDS             complement(2203..>2584)
                     /locus_tag="VFU75_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002216361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU5465596.1"
                     /translation="RIVAAFTYMAHGTQKLFGFPGGVAHVEYLSLYGLAGVIETAGGL
                     FLLLGLFTRPVAFITAGEMAVAYFLVHFRQGVWPLLNHGELAVVYCFLFLYIATVGPG
                     PWSLDRVLGPGESDVRSTGARRLK"
BASE COUNT          420 a          851 c          910 g          403 t
ORIGIN      
        1 tccggctgcc cgcacttcgc atacgcgatg ccggcgaggg tccgcacccc ttcgtagaaa
       61 ttcgtcatcg gccggctcgt gatccgaatg gcgtcgtcgc agcggcccag catcaggtag
      121 gagcgggcga ggtccggcac cgccaatgcg tacgtggaat ccccggccaa taccagctca
      181 ccttcccgcg cggcctcctc ttctctcccc gcgaaataga gtgccgacat gagccgagtg
      241 ccgacgatca gactgaaggg ctcgagcgcc tgggccgcac gcatttccgc gacggcggaa
      301 tcggactgat gctgggtaat gaagacgtgg gaccggaaca ggtgcgcgcg ggtcagcgtc
      361 gagtccagcc gcagggcctc gtcgagctgg cgcagcgcat ccggaacgtt ccaatcgtag
      421 aagagcgcga cgaatcccag gctcgcgtac gcctccggca ggagactgtc tcgagccacc
      481 gcttcgttgc cggcggcgcg ggattgcggg aagtagtcgc gcggcgggcc gtattggaac
      541 gccgccatga atccataggc gtcggcaagc cccgcgtagg ccagcgcgaa gttcgtgtcg
      601 gcgtgaatcg cggcgtggaa caactggatg gaccgcagga gcgccgcctg ggtccgctgc
      661 tcgagggccg accgcccttg cagatagaga tcgtaggcgg cggggttccg ggtcgggcgg
      721 cgagccaggg aggccgcggt ggcggcgctg agattcaccc ggagctgaga tgtgatactc
      781 cgcacgatct ccgcctgcac gtcgaggttg cctcgatcca acgtgtcgta cgtcgccggg
      841 agaatggtgt ccccggtagc agcccggatg aggtagctct gcaccttcac ccggctgccc
      901 tgccggagta cgctgccgcc gatcaggtac gaggcgccga ggcgctgtcc caactcctgg
      961 ggagtgcggg cggtccgggc gaaccggtag gatgacgtcg ccggggccac ctgcaaaccc
     1021 ggaatccgcc gcaacgcgcc gatcaggtct tccgtgaagc cgtcgacgta gtagtccggc
     1081 ctccccgccc cactggcgtt ggcgaacgga atgaccgcga ccgatgcagg cgtgatggtc
     1141 gccgccgccg gccggtgcgg ccggcggacc atggtggtcg ccgccgcggc ggcgatcacc
     1201 gtgaacgtgg cgagcatcgc gaggcgccgc cctctgcggc gatggggtgg ctcggcccgc
     1261 gccacccgac gggtgacgac ttcgcccgtg atggatcccc ggagcgcctt gtcgagcgcc
     1321 tcggcattcg ggtagcggtc ggcaggctgc ttggccagcg agagggtcac cacctgctcg
     1381 agcgcgtccg gtacgtcggc gcgtaccgcc cgaatggacg gcaccggatc gagcgtatgc
     1441 ttggccagaa cggcctgcgc cgtggttccc ccgaacgggg gccgtccggt gagcatctcg
     1501 tagagcacgc atcccaggct gtactggtcg ctccgcccgt ccaccgcctc ggccaatgcc
     1561 tgctcgggac tcatgtagac cggggtgccg accgtgatcc ccgcggtggt gacgggtccc
     1621 gaagcttccg cggcggtgat ggcacgcgcg atcccgaagt cggcgaccag tgcgtagcct
     1681 tcggacagga ggatgttctc gggcttgatg tcgcggtgaa cgatatcgcg cgcgtgggcg
     1741 tagctcagcg cgctcgcgac ctcccccccg atctgcacgg tgtcgtgcaa cgacatctgc
     1801 ggctcccggt cgagccgctg gcggagcgat tcgcccgtga cgtacggcat cgcgtagtac
     1861 aggaggtcgt tcgactcgcc ggagtcgtac agcggcagga tgtgcggatg gttgagctgg
     1921 gcggcgatct ggatttcgcg caagaaccgc gcggcgccga tcgctccgga gatttcgggg
     1981 cgcaggacct tgatggcgac ggggcggctg tgtttctcgt ccaacgcgag gtacacgatg
     2041 gccatgccgc cgcggcccag ctcccgctcc acgcggtagc gtccggcgag accctggctc
     2101 agtcgggcgg cgatggtgtc gcgagatggc acggcgtgca aagtacgcca agatccttcg
     2161 cttcgctcag gacaagcgcc tgcctacgcc tgcggcgcct acctacttca atcggcgcgc
     2221 tccggtgctg cggacgtcgc tctcccccgg gccgagtacc cgatccagac tccagggacc
     2281 gggaccgacg gtggctatat agaggaacag gaagcagtag accacggcca gctcgccgtg
     2341 gttcaggagg ggccagaccc cttgccggaa gtgcaccagg aaatacgcga ccgccatctc
     2401 ccccgccgtg atgaaggcca ccggtcgcgt gaacagtccc aggagaagga agaggccccc
     2461 ggccgtttcg atcactccgg ccagtccgta gagggagagg tattcgacgt gagcgacgcc
     2521 cccggggaaa ccgaacagct tctgggtgcc gtgcgccatg taggtgaacg cggcgacgat
     2581 gcgc
//
LOCUS       DASUBP010000040        10226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_300657, whole genome shotgun sequence.
ACCESSION   DASUBP010000040 DASUBP010000000
VERSION     DASUBP010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 10226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10226
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300657"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1351)
                     /locus_tag="VFU75_01845"
     CDS             complement(<1..1351)
                     /locus_tag="VFU75_01845"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU5465597.1"
                     /translation="MTAPPDGRPRRPRQLQLMGLVAVMYFCVSGGPFGLEGMVGPVGP
                     GLALLLLVVIPILYTIPEALLIGELASMLPAEGGYYVWVKRAFGPFWGFWNGWLSWVY
                     SLVDMALYPVLFRDYLHYFLPGLTPLEGWIVALSVIWLATGLNLRGTRTVGAASTGFV
                     ALVVAPFAVLAALAAVKWLGDPAAHLSLTPFHAEGTTFFAALGVGISQGIWNYSGWDN
                     ASTIGDEIQSSGRTYPKALARAVPLVTLVYLVSVVPTLAVSDWHIWTDGAWPAIARAI
                     VGGWLAQWIALAGMVSAFALFNALLLAYSRIPLALARDGLLPRSLGGTDARGTPRTAV
                     IVSAIIYSVWVLLPFGDLLAADVLLYTLALMLEFGALLALRRKEPGLRGPFRVPLPTS
                     VLALLAALPPLVLGTAVALELTGEGAGFKGVLVGLALAAVGPVIYAVLIRRAPRAAKA
                     "
     gene            complement(1348..2097)
                     /locus_tag="VFU75_01850"
     CDS             complement(1348..2097)
                     /locus_tag="VFU75_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465598.1"
                     /translation="MRPLTLVLLMAAVACHPPGGHAVATPDTTDATAAGAADREMSTL
                     AGMSADPHMAMTPTWPLAAGDSARARALVDQIRASFAKYKDVRVAEADGFKEFLPNVK
                     NQKVYHFTSTRNAITARFSFDPLAPTSLLYKEEAGGGMTLVGVMYTETPRASLEELNA
                     RVPLSITHWHQHVNWCLPPRDRPARWRDSTGGKPVFGPLGPIATQAACDSAGGRFFPR
                     VFGWMVHVNAFSGDDPKVIWGGEDHDDHMKM"
     gene            complement(2141..5221)
                     /locus_tag="VFU75_01855"
     CDS             complement(2141..5221)
                     /locus_tag="VFU75_01855"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEU5465599.1"
                     /translation="MILLTLFLFPALAAGQTPTVDTARYAGLRWREIGPFRGGRVDAV
                     AGVVGQPLIYYMGATGGGIWKTVDGGNTWEPVADKYLTSGSVGAIAVAESDPNVIYAG
                     TGEETVRGNVSPGDGMYKSTDAGKTWSKIGLADAGQVGRIAVNPKNADLVYVAVMGHA
                     FGPNAMRGLYRSNDGGKTWQKVLSRNDSTGAVDVVLDPTNPRIIYAALWQEQRTPWDF
                     SSGGAGSGLFKSTDGGDTWTELTRKKGMPEGVIGRIGIAVSPVSPDRIWAVVEAKEGG
                     VFRSDDAGDTWHKTSDDHEIRQRAWYFSNVFADPASADVVYALNVSFRKSVDGGVTFE
                     GMDVPHGDNHALWIDPANPRRMVLGNDGGATVTVDGGKSWTTLDNQPTAQFYHVIATT
                     HWPYQVCGAQQDNSTVCIATSSDGSGITDRDWEDVGGGESGWIAARADTPDVVFAGSY
                     GNHITRLDRRNHQERNINAWPDNPMGHAASDLKYRFQWTFPIVIAPTNSADLYIGSQI
                     LFKSTNEGSSWQAISPDLTRNDTTRQRSSGGPITKDNTSVEYYGTIFTIAPSPRDSNL
                     IWTGTDDGLVQVTRDGGKTWTNVTPPGVTAGTQISMVDASVHDAGTAYVAANRYKLDD
                     FAPYAFVTHDFGKTWKKIVAGIPATHFVRVVRQDPVRPNLLYAGTEFGAYVSFNDGAS
                     WQSLRLNLPVVPVHDLTIKDNDLIAATHGRAFWIIDGVGPLRELAGTEAQADRVLYQP
                     EDPWRTGGFRAGRRQATVGQNAPRGAQIFFYLKDKPDSAVTLEIRDAHDSLVRRWSTK
                     PAEPQDSLEVKAGMNHFAWDLRYPGAHRFKGMILWGGGTGGPVAVPGTYSVRLHTAGW
                     TASRTFQVKEDPRVRVTTADLQKEFDLLIRIRDRLSQADDAVKQIRDLVSQLDGVETR
                     VKGQPGAAGIDARADSLKTRLGVIEKAIYQTQNQSGEDPLNYPIRLNNRIAGVAGVVG
                     SADAAPTEQSYQVFELVSGLLQTQLDQLKAIVTTDIPAFNQAVAAQNVPAVIVR"
     gene            5490..6359
                     /locus_tag="VFU75_01860"
     CDS             5490..6359
                     /locus_tag="VFU75_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465600.1"
                     /translation="MSVKLAIAVVAAAVVAAGCDQGALKVAQARADSLQNVAADHDRA
                     MAEVAADAKALSDISGEIASVRLNGKMKIQPAESPDAARRDTVVEKVRLLVSRVHNAD
                     AQVKNSKTEIAQMTHLSDSLRATLESTVTNFQKLVDDQKTQIATLTARVDSLTGVNIA
                     LNDTLNATTTRDNTVYYVIGTKDDLIKKGVITEEGGARFLFVLWKSGKTLVPARELDP
                     AQFTAIDQRQVTSIALPDSSQAYRIVSRNDLTALNTPPDPNSGEIHNTNSLAIAKPGQ
                     FWAGSRYLIIVEG"
     gene            6484..7539
                     /locus_tag="VFU75_01865"
     CDS             6484..7539
                     /locus_tag="VFU75_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465601.1"
                     /translation="MIAVAYLTLAALQHLGPLLDVAPPPPPVSPAPAPVVSAAFFATA
                     DTGGRRPRAIEYSDFYGVRLEIHKAASYATLPLVIGEYALGQSLYNHPPGTSATLTAH
                     QITALGLAGLFGVNTVTGAWNLWDSRHDPNDKARRYIHAALMFISDAGFVAAGASTPS
                     PRRPVSRYDPPHHRAQRHGSGPRRIRDDADMEEMSLVARWAHLYSDHKAVSEAVNFIH
                     FGGVLLAGGLAVAADRATLRLSRAPVDGGTSSWALAEIAGVHRLVLTGLTVVVVSGLL
                     QTFADLDTYLTSVVFWSKMGLVAVLLGNGWIRLRAENGVRRGEHGAWPAYRRTSVASL
                     ILWFTLVLLGTVLSGNG"
     gene            7536..8048
                     /locus_tag="VFU75_01870"
     CDS             7536..8048
                     /locus_tag="VFU75_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske 2Fe-2S domain-containing protein"
                     /protein_id="HEU5465602.1"
                     /translation="MTKKITEACEQCPLESRRDFMRDATVAVAGVVAALGIGSADARA
                     MTWRLVKPVRSEDEEHSYPIPASDDAMIDKQNQVIIVRWQGKAYGFNLSCPHQNTALH
                     WHPEDSQFQCPKHHSRYQPDGVFISGRATRSMDRFGVRRDGDNIVVDVDKYYRQDQNA
                     AEWTAAFLTL"
     gene            complement(8064..9008)
                     /locus_tag="VFU75_01875"
     CDS             complement(8064..9008)
                     /locus_tag="VFU75_01875"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU5465603.1"
                     /translation="MKKILAVSAVCVALLAQNAAAQYGGGRSGGMRGGFAQPMEPAPP
                     LPGAVLDGPPDSAAARSALALTDSQAVKYALSYRSFMTATKQSRDSAQVATDKMNDRL
                     ATGDRAAATFYAQRLQDIGKSLRDRQDKFDDGLRSFLTKDQQKAYKKWEDDAERVAQE
                     RNKEAGLRWQEAGGFGGGRATDLRIPVEATDAPPATLGSQAVRIGRTIYVSAQLGVDT
                     TGALKGADLAAQAKQAFDNVTAILKRATASPGDVVRVTIYLINPDSGAAAILRSAAPE
                     YFSGRNQPATAILGVQSLTRPGALVAVEVTAVTGSSGF"
     gene            complement(9112..9657)
                     /locus_tag="VFU75_01880"
     CDS             complement(9112..9657)
                     /locus_tag="VFU75_01880"
                     /inference="COORDINATES: protein motif:HMM:NF018257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfocyanin-like copper-binding protein"
                     /protein_id="HEU5465604.1"
                     /translation="MNTPIRSFVIAAALMGAAAPCTLSAQGAPLKVDPSWVTTRISDS
                     TVEFRLVAGMSPANGGMNFDGATGGSLTLTVPVKWHVVLHFRNDDENMPHSAEVTIAK
                     SPVPAMPDTKPVFGGAESKDATQGVSVGTKQDLHFTADQPGSYIIVCAVPGHGAAGMW
                     IRLVVSATASKPDVEAGPATP"
     gene            complement(9654..>10226)
                     /locus_tag="VFU75_01885"
     CDS             complement(9654..>10226)
                     /locus_tag="VFU75_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465605.1"
                     /translation="LAGAAPYWRVAIPNTIDGKYLSLGAYGMSSRIYPAGVTGPTNRF
                     TDVAADLSFLWPMGENSFTTEATYIHESQRWDAGGSANSTNTLKTFRFDMMYHVGHTY
                     AFTAAPFFTSGSSDTLLYAAAPMSGSRIGSPNSSGFIGEVDVMPWQNLRFQLQYVAYS
                     KFNGASTNYDGAGRNASDNNTLYFLTWVLF"
BASE COUNT         1721 a         3477 c         3305 g         1723 t
ORIGIN      
        1 ccgccttcgc cgcgcgtggg gcccgtcgga tcaagacggc atagatcacc ggccccaccg
       61 ccgcgagcgc cagtcccacc agcactccct tgaaaccggc gccttcgccg gtcagctcca
      121 acgccaccgc cgtccccagc accagcggcg gaagcgccgc gaggagggcc agcaccgacg
      181 tcgggagcgg cacccggaat ggtccgcgca ggccgggctc cttccgccgc agggccagca
      241 gtgcgccgaa ctccagcatc aaggccagag tgtagagcag gacgtccgcc gcgagcaggt
      301 ctccgaacgg caagaggacc cacaccgagt agatgatggc ggacacgatc accgctgtac
      361 gtggcgtgcc ccgtgcgtcg gtcccgccta acgaccgggg caagagtccg tcgcgcgcga
      421 gggcgagggg aattcgggaa taggcgagca ggagggcgtt gaagagcgcg aaggcgctca
      481 ccatgccggc cagggcgatc cactgcgcca gccacccccc cacgatcgcc cgcgcgatcg
      541 ccggccatgc gccgtcggtc cagatgtgcc aatcactcac cgccaaggtc ggcacgaccg
      601 agacgagata gacgagcgtc acgaggggga ccgcgcgcgc cagtgccttc ggataggtgc
      661 ggcccgacga ctggatctca tcgccgatcg tcgacgcgtt gtcccaaccg gagtagttcc
      721 agatcccctg actgatcccc acgcccaggg ccgcgaaaaa cgtggtgcct tcggcgtgaa
      781 agggcgtcag ggacagatgc gccgccgggt cccccagcca cttcacggcc gcgagggcgg
      841 ccagcacggc gaagggtgct accaccagag ccacaaatcc cgtcgacgcg gcgccgacgg
      901 tacgggtacc ccggagattc aggccggtgg caagccagat gacgctgagg gcgacgatcc
      961 agccctcgag cggcgtgagc ccggggagga agtagtggag gtagtcgcgg aagaggaccg
     1021 ggtagagcgc catgtccacc agcgagtaga cccaggagag ccacccgttc cagaaccccc
     1081 agaagggccc gaaggcgcgc ttgacccaga cgtagtagcc gccctccgcc gggagcatcg
     1141 acgccagctc gccgatcagc agggcttccg gaatcgtgta gagaatgggg atgacgacca
     1201 acaagagaag ggcgagaccc ggcccgaccg gtcccaccat cccctcgagc ccgaatgggc
     1261 ctcctgacac gcagaagtac atcacggcca cgagccccat caactggagc tggcgtggcc
     1321 gtcggggccg cccgtccggt ggtgcggtca catcttcatg tggtcgtcgt gatcctcacc
     1381 gccccagatg actttggggt cgtccccgga aaacgcgttc acgtgcacca tccacccgaa
     1441 cactcgcggg aagaagcggc caccggcgct gtcacaggcg gcctgcgtcg cgatcggtcc
     1501 caggggaccg aagaccggtt tcccgccggt agaatcccgc caccgagcgg gccggtcgcg
     1561 gggggggaga caccagttca cgtgctggtg ccaatgggtg atgctcaggg ggacgcgggc
     1621 gttcagctcc tccagggacg cccgcggcgt ctcggtatac atgactccca ccagggtcat
     1681 cccgcccccg gcctcttcct tatagaggag agacgtggga gccaggggat cgaaggagaa
     1741 tcgtgcggtg atcgcgttcc gcgtgctcgt gaaatggtag accttctggt tcttcacgtt
     1801 cggcaggaat tccttgaacc catcggcttc ggccactctg acgtccttgt acttggcgaa
     1861 gctcgcccgg atctggtcga ccagggcccg cgcccgtgcg gagtcccccg cggcgagcgg
     1921 ccacgtgggg gtcatcgcca tgtgaggatc ggcactcatc cccgccaggg tggacatctc
     1981 ccgatccgcg gcacctgccg ccgtggcgtc cgtggtatca ggcgtcgcca cggcgtggcc
     2041 gccgggaggg tggcaggcca ctgcggccat cagcaatacg agcgtcaacg gtctcatatc
     2101 agcaccagag atctggagtc atcccaggta cggataggga tcagcgaacg atgaccgccg
     2161 gtacgttctg cgccgctacc gcctggttga acgcagggat gtccgtcgtc acgatggcct
     2221 tcaactggtc cagctgcgtc tggagcaggc ccgacaccag ctcgaatacc tggtacgact
     2281 gttccgtggg cgccgcatcg gcgctcccga ccacgcccgc cacacccgcg atccggttgt
     2341 tgagccggat gggatagttg agcgggtcct cccccgactg gttctgcgtc tggtagatcg
     2401 ccttctcgat cacacccaga cgcgtcttga gcgagtccgc ccgggcgtcg attcccgcgg
     2461 cccctggctg acccttcacg cgtgtctcca cgccgtccag ttggctgacg aggtcgcgga
     2521 tctgcttcac cgcgtcgtcg gcctgcgaaa gccggtcgcg gatccggatc aggaggtcga
     2581 actccttctg gagatccgcc gtcgtcaccc gcacccgcgg gtcttccttc acctggaagg
     2641 tcctcgaggc ggtccacccc gccgtgtgga gccggacgga ataggtgccg ggcacggcca
     2701 ccggaccgcc ggtaccccca ccccacagga tcatgccctt gaaccggtgc gcgccagggt
     2761 aacggaggtc ccatgcgaaa tggttcatcc ccgccttcac ctcgagcgag tcttgcggtt
     2821 ccgccggttt ggtcgaccac cggcgaacga gcgagtcgtg agcatcccgg atctccagcg
     2881 tgaccgccga gtcgggcttg tccttcagat agaagaagat ctgggcaccg cgcggggcgt
     2941 tctgcccgac ggtcgcctgg cgccggcccg cccggaagcc accggtgcgc cacgggtcct
     3001 cgggttggta gagcacccgg tcggcctgag cctccgtacc ggccagctcg cgcagcggtc
     3061 ccacgccgtc gatgatccag aacgcgcgcc cgtgcgtcgc ggcgatcagg tcgttgtcct
     3121 tgatcgtgag atcgtgcacg ggcaccaccg gcaagttcag ccgcagcgac tgccagctcg
     3181 cgccgtcgtt gaaggacacg taggccccga actcggtacc ggcatacagc aggttgggcc
     3241 gcacggggtc ctgccgcacc acccgcacga agtgcgtcgc cggaatcccg gcgacgatct
     3301 tcttccaggt cttgccgaag tcgtgcgtga cgaacgcgta gggcgcgaag tcgtccagct
     3361 tgtagcgatt ggcggcgacg tacgcggtac cggcgtcatg gaccgaggcg tccaccatgc
     3421 tgatttgcgt gccggcggtc acccccggag gcgtgacgtt ggtccaggtc ttcccgccat
     3481 cgcgagtcac ctgcaccagc ccgtcatcgg tgcccgtcca gatgaggttc gaatccctgg
     3541 gcgacggcgc gatggtgaag atggtcccgt agtactcgac cgaggtgttg tccttcgtga
     3601 tcggcccgcc cgaggaccgc tggcgcgtgg tgtcattccg cgtcaggtcg gggctgatcg
     3661 cctgccagct cgagccctcg ttggtgctct tgaagaggat ctgcgacccg atgtagaggt
     3721 ccgcggagtt cgtgggggcg atcacgatcg ggaaggtcca ttggaagcgg tacttcaggt
     3781 ccgatgccgc atggcccatg gggttgtcgg gccaggcgtt gatgttccgc tcctggtggt
     3841 tccggcggtc cagccgggtg atgtgattgc cgtagctgcc cgcgaagacc acgtcgggcg
     3901 tgtcggcgcg cgccgcgatc cacccggatt cgccgccccc cacgtcctcc cagtcccggt
     3961 ccgtgatccc gctgccatcc gatgatgtcg cgatgcagac ggtgctgttg tcctgctgcg
     4021 ccccgcagac ctggtagggc cagtgggtgg tcgcgatcac gtggtagaac tgggccgtcg
     4081 gctgattgtc gagcgtggtc cacgacttcc cgccgtccac cgtcaccgtg gccccgccgt
     4141 cattgcccag caccatgcgc ctgggattcg ccggatcgat ccagagggcg tggttgtcgc
     4201 cgtgggggac gtccattccc tcgaacgtca cgccaccatc cacggacttg cggaacgaca
     4261 cgttcaacgc gtagaccacg tcggcactcg ccgggtccgc gaagacgttg ctgaagtacc
     4321 aggcccgctg gcggatctcg tggtcgtcgg aggtcttgtg ccaggtgtct cccgcgtcgt
     4381 cggaccggaa gacgcccccc tccttggcct ccaccaccgc ccagatccgg tcggggctca
     4441 ccggcgagac cgcgatgccg atgcggccga tgacgccctc cggcatgccc ttcttccgcg
     4501 tgagctcggt ccaggtgtct ccgccgtccg tgctcttgaa gagtccactc cccgcgccac
     4561 cggacgagaa gtcccagggg gtccgctgtt cctgccagag tgcggcgtag atgatccgtg
     4621 gattggtggg atcgagcacg acgtccacgg cacccgtcga atcgttgcgg gagagaacct
     4681 tctgccaggt cttcccgccg tcattcgagc gatagagtcc acgcatcgcg ttcggaccga
     4741 acgcgtgtcc catgacagcg acgtagacca ggtcggcgtt cttcggattg acggcgatcc
     4801 ggccgacctg ccccgcgtcg gcgaggccga tcttgctcca cgtcttcccc gcatcggtcg
     4861 acttgtacat cccgtcaccg ggcgagacgt tgccgcgtac cgtctcctcc ccggtgccgg
     4921 cgtagatcac gttcgggtcg gactcggcca cggcgatcgc gcccacggac ccggacgtca
     4981 ggtacttgtc ggcgaccggc tcccaggtgt tcccgccgtc gaccgtcttc cagatgccgc
     5041 cgccggtcgc tcccatgtag tagatgaggg gctgaccgac gacgcccgcg accgcgtcga
     5101 cgcggccgcc gcggaaggga ccgatctcgc gccaccggag tccggcgtaa cgcgcggtgt
     5161 ccaccgtcgg agtttgtccg gcggcgagcg ccgggaacag aaaaagcgtg aggagaatca
     5221 caacgtgttg ggcacgatac gaacgtgaca tgtgcgcgcc actcggctgg aggtttcggg
     5281 gttgcaactc ttcggatcga ggtgcaatga tacgggcccg acgtcgggca cgcgagttgc
     5341 cgcctttcag ggaattgaaa cgtgtgccga atgccggtgt acgaccgacg ctacaccgga
     5401 ttcacgtcgg accgatcgac cagcccggaa ctgccatgcc cgcacgcccg gggggccaat
     5461 acggatcact tttttgggag gatgcgctta tgtccgtcaa actcgctatc gcggtcgtcg
     5521 ccgccgccgt ggtcgcggcc gggtgcgacc agggagccct gaaggtcgcg caggcgcgcg
     5581 ccgacagtct gcagaacgtc gccgcggatc atgaccgcgc gatggcagag gtcgccgccg
     5641 atgccaaggc cctgtccgac atctccggcg aaatcgcatc cgtgcggttg aacgggaaga
     5701 tgaagatcca gcccgccgag tcgcctgacg ccgcgcggcg cgacaccgtg gtcgagaagg
     5761 ttcgcctgct cgtgtcccgc gttcacaatg ccgacgccca ggtgaagaac agcaagaccg
     5821 agatcgcgca gatgacgcac ttgtccgatt cgctgcgcgc gacgctcgag agcaccgtga
     5881 cgaatttcca gaagctggtg gatgatcaga agacgcagat cgccacgctc accgcgcggg
     5941 tcgattcgct taccggcgtg aacatcgcgt tgaatgacac cctgaacgcc accaccaccc
     6001 gcgacaacac ggtctattac gtcatcggga ccaaggatga tctgatcaag aagggcgtga
     6061 tcaccgaaga gggtggggcc cggttcctgt tcgtcctttg gaaatcgggc aagacgctgg
     6121 tgccggcacg ggagctcgac ccggcgcagt tcaccgcgat cgatcagcgc caggtgacga
     6181 gcatcgcgct gccggactcg tcgcaggcct accgcatcgt gtcccgcaac gacctcacgg
     6241 cgctcaacac cccacccgat cccaacagtg gcgagatcca caacaccaac agcctcgcca
     6301 tcgccaagcc cgggcagttc tgggcggggt cgagatatct gatcatcgtc gaaggctgaa
     6361 cgctgcgatt tgaaacgagc gccccggcat ttccgtgccg gggcgttcgt gtgttcatca
     6421 cccctcgtga ggaacccgta ctttgggtgg tgtagagact ccgacccgtt cctgggagac
     6481 cggttgatcg ccgttgcata tctcacactg gcggccctgc agcacctggg cccgttgctg
     6541 gacgtcgctc ctcccccccc gccggtgtca cccgcgccgg cgcccgtcgt ttccgccgcc
     6601 tttttcgcga ccgcggacac cggggggcgc cgcccgcgcg ccatcgaata cagtgacttc
     6661 tacggcgtcc gtctcgaaat ccacaaggcg gcgagttacg ccaccctgcc cctcgtcatc
     6721 ggtgagtacg cactcggcca atccctctac aaccacccac cagggacgtc cgccaccctt
     6781 accgcccacc agatcacggc gctcggcctc gccggactgt tcggggtgaa caccgtcacg
     6841 ggggcatgga atctctggga cagccggcat gaccccaatg acaaggccag gcgctacatc
     6901 cacgccgcgt tgatgttcat ctccgacgcg gggttcgtcg ccgcgggggc ctcgacgccg
     6961 agtcctcgcc gacccgtcag ccgctacgac ccaccgcacc atcgcgctca gcgccatggg
     7021 agtggccctc gtcggatacg ggatgatgct gatatggaag aaatgagtct cgtggcgcgc
     7081 tgggcccacc tctacagcga tcacaaggcc gtgtccgaag cggtgaactt catccatttc
     7141 gggggagtcc tgctcgccgg cggactcgcc gtggccgcgg atcgagcgac gctccgcctg
     7201 tcccgcgccc ccgtggatgg gggaacctcc tcctgggctc tcgccgaaat cgccggagtc
     7261 caccggctgg tgctcaccgg gctcacggta gtggtggtca gtggcctgct ccagaccttc
     7321 gcggacctgg acacctacct cacctcggtc gtcttctggt ccaagatggg tctggtcgcc
     7381 gtgctcctgg gcaacgggtg gatccgtctc cgggctgaga acggggtgcg gcggggggag
     7441 cacggtgcct ggccggcgta tcgtcgcacg tcggtggcca gcttgatcct gtggttcact
     7501 ctggtgttgc tcggaaccgt cttgtcgggg aacggatgac caagaagatc accgaagcat
     7561 gcgagcagtg tccactcgag tcgcggcgtg acttcatgcg ggacgccacc gtggccgtcg
     7621 ccggtgtcgt ggccgcgctc gggatcggat ccgcggacgc ccgcgccatg acctggcgcc
     7681 tggtgaagcc cgtccgctcg gaggatgagg aacactcgta cccgatcccc gcatccgatg
     7741 acgcgatgat cgacaagcag aaccaggtca tcatcgttcg gtggcagggc aaggcgtacg
     7801 gcttcaacct ctcctgccca catcagaaca cggccctgca ctggcatccc gaagacagcc
     7861 agttccagtg cccgaagcat cattcgcggt atcaacccga cggcgtgttc atctcgggac
     7921 gcgccacgcg aagcatggac cggttcggtg tccgccgcga cggcgacaac atcgtagtcg
     7981 acgtggacaa gtactaccgg caagatcaaa acgccgcgga gtggaccgcg gcgtttctga
     8041 ccctctagtc cttcagcggc cgatcagaac ccgctgctcc cggtcaccgc ggtcacttcg
     8101 accgcaacga gggctccggg cctggtcaag gactgcacgc ccaggatcgc agtggccggc
     8161 tggttccggc cggagaagta ctccggggcc gctgaccgca ggatcgccgc cgcaccggag
     8221 tccgggttga tgaggtagat cgtcacacgg accacgtcac ccggtgacgc cgtcgcacgc
     8281 ttcaagatgg cggtgacgtt gtcgaaggcc tgtttcgcct gggcggcgag atccgcaccc
     8341 ttcaaggccc cggtcgtgtc cacgccgagc tgagccgaca cgtagatggt ccggccgatc
     8401 cggacagcct gtgaccccag cgtcgccggc ggcgcgtcgg tagcctcgac cggtatgcgc
     8461 agatccgtgg ctcggccacc cccgaagcca ccggcctcct gccaccgcag gcccgcttcc
     8521 ttgttccgct cctgcgccac ccgctcggcg tcatcctccc acttcttgta ggctttctgc
     8581 tggtccttgg tcaggaagga cctcaagccg tcgtcgaatt tgtcctgccg gtcccgcaac
     8641 gacttcccga tatcctggag ccgctgagcg tagaaggtcg cggccgcgcg gtcccccgtg
     8701 gcgaggcggt cattcatctt gtccgtcgcc acttgtgccg agtcgcgcga ctgcttggtg
     8761 gcggtcatga aggaccggta cgacagggcg tatttcacgg cctgcgagtc cgtaagcgcc
     8821 agcgcactcc gcgcggccgc cgaatcgggg ggaccatcga gcacggcgcc cggcagcgga
     8881 ggcgccggtt ccatgggctg ggcgaacccc cctcgcatgc cgccgcttcg gccaccgcca
     8941 tactgcgctg cggcattctg cgccagcaag gccacacaga ccgccgaaac cgccaggatt
     9001 tttttcataa gggctttata ccgtgaagag ctcgtgtagt tctcgcggac ggaaacacgt
     9061 acggcggcca accgagacga gttgaccgcc gcatggtgta cggcccgacc ctcagggtgt
     9121 cgccggccct gcctctacat ccggcttgga tgccgtcgcg ctgaccacca ggcggatcca
     9181 catcccggca gcgccatgac cgggcacggc gcacacgatg atgtacgacc ccggctggtc
     9241 cgccgtgaag tggagatcct gcttcgtccc cacgctcaca ccttgcgtcg cgtccttgct
     9301 ctcggcgccc ccgaacaccg gcttggtgtc gggcatggcc ggaaccggag acttggcgat
     9361 ggtgacctcc gcggaatgcg gcatgttctc gtcgtcattc cggaaatgca ggaccacgtg
     9421 ccatttcacc gggacggtga gcgtcagtga tccaccggtg gcaccgtcga agttcatgcc
     9481 accgttcgcc ggcgacattc ccgccaccaa ccggaattcg accgtggagt ccgagatcct
     9541 cgtagtcacc cacgaggggt cgaccttgag gggagcaccc tgcgccgaca gcgtgcaggg
     9601 ggccgccgcg cccatcagcg cggcggcgat gacgaaactg cgtatcggtg tgttcaaaag
     9661 agtacccagg tcaggaagta gagagtgttg ttgtcggatg cgttgcgccc cgctccgtcg
     9721 tagttggtcg aagccccgtt gaacttcgag taggccacgt actgcagttg gaagcgcagg
     9781 ttctgccacg gcatgacgtc cacctcgccg atgaatccgc tcgagttcgg gctgccgatg
     9841 cggctcccgc tcatcggggc ggccgcgtag agcagggtat cactgctccc actcgtgaag
     9901 aagggcgcgg cggtgaacgc gtacgtgtgt cccacgtgat acatcatgtc gaagcggaag
     9961 gtcttgagcg tgttggtcga attcgccgat cctccggcat cccagcgctg tgattcgtgg
    10021 atgtacgtcg cctcggtggt gaagctgttc tcccccatcg gccagaggaa gctgagatcc
    10081 gctgcgacat ccgtgaatcg attggtgggt cccgtgacgc ctgcgggata gatgcgtgac
    10141 gacatcccgt acgcgccgag cgacaggtac ttcccgtcga tcgtgttcgg aatcgcgacg
    10201 cgccagtacg gcgctgctcc agccag
//
LOCUS       DASUBP010000041         6663 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_464277, whole genome shotgun sequence.
ACCESSION   DASUBP010000041 DASUBP010000000
VERSION     DASUBP010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6663)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6663)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6663
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464277"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..397)
                     /locus_tag="VFU75_01890"
     CDS             complement(50..397)
                     /locus_tag="VFU75_01890"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEU5465606.1"
                     /translation="MKIRTKRVYDPPATGDGRRILIDRLWARGLSKGKARIDYWAKTI
                     APSTALRKWYGHDPEKWAEFRKRYFAELDANPEGVAELKTEMGKGTVTLLFSSRETRL
                     NNASALREYLEAK"
     gene            complement(394..1023)
                     /locus_tag="VFU75_01895"
     CDS             complement(394..1023)
                     /locus_tag="VFU75_01895"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5465607.1"
                     /translation="MVFIMNQCRKPSGAMGQRVLQAMNLRHSTVTDWGLGHTRIEPSF
                     QILDVGCGGGRTVQKLAGLAPHGHVSGIDYSDASVAEARRVNQAEIAAGRVDIQEASV
                     SHLPYADAAFDLVTAVETHYYWPDLVADLREVRRVLKPGRSLVIIAETYRGQAFGLLL
                     RLPMMLLRAKYLTVDQHREALTAAGFAEVEISVERWKGWICVVGRKPGS"
     gene            complement(1250..2482)
                     /locus_tag="VFU75_01900"
     CDS             complement(1250..2482)
                     /locus_tag="VFU75_01900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465608.1"
                     /translation="MKKIIPLVFAAVVVGVAVAAFRAPAPSSYLFVWAGANDTASDFL
                     AVIDADPGSPHYGSVVTSLAVGAAGTHPHHTEAVMPADGHLLANGFHAGKTWLFDLTK
                     PAAPRILTSFGDLGGFSHPHTYVRLADNTILATFQYAAGAGGMSMGPNTTGGLVHMDE
                     RGHVLATGSARDSAVSERLISPYSVLPIPSLNVAVSTTTDMDLTDTAANQWVQLWRLS
                     DLKLQKSIALPPGPAGKENQLTGEPFLLPDGHSVYIHTFRCGLYLLRGVETGAPSAEL
                     VKTFAGTPCGVPILTGHWWIQTVSSEHKLVALDVSDAEHPREVSEATLGDDESPHWMA
                     IDPTGRRLVVNSGGYAKGDRLFIVNFDPATGTLSVDSEFKDPGASVPGLDLSHRTWPH
                     GYTGRAAPHGAVFSLPVR"
     gene            complement(2530..2685)
                     /locus_tag="VFU75_01905"
     CDS             complement(2530..2685)
                     /locus_tag="VFU75_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465609.1"
                     /translation="MKLSRNLAFFLLGAWLILNGLISMIHLSFSGLSLIMSILAVAAG
                     ALLIIGK"
     gene            complement(2707..3129)
                     /locus_tag="VFU75_01910"
     CDS             complement(2707..3129)
                     /locus_tag="VFU75_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465610.1"
                     /translation="MDRTNRLRVTLHGAVVMLIGLFCGFPTVLESDAERYWHTAHEAL
                     ILTGTLMLAVSAVMSQLVLAKREALGLFWALLATGYGLSVGTVLQGILGQHAFGPSSS
                     PMVMIAFIANGVGIGGSVISTALVVMGAAAALKASPKD"
     gene            complement(3209..4240)
                     /locus_tag="VFU75_01915"
     CDS             complement(3209..4240)
                     /locus_tag="VFU75_01915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU5465611.1"
                     /translation="MKALQLVAIGAPVALRDVPEPAFGPNDVLIRVHAAGICHSDSHY
                     RAGRSPAGPLPLTLGHEVAGVVERVGVEVKAPQPGDRVCVHYLISCGGCPACVRGDEQ
                     FCATGAMIGHHRAGGWAEFIAVPAVNAVPLPAGIPFAHGAVAMCSAATAFHALKRGRL
                     AKGDRVAVFGIGGLGMSAVQLARALGAAEVFAVDLDERKLKLAESFGAVTIRAAVGDP
                     VGKIREHTGGRGVDVALELIGLPLTMEQSLKVLAVHGRAVVAGLATKPMSVDTYRDLL
                     GREAELLGANDHLKSELPALLDLIEKGSVDLERVVERRVTPDTRVVNEILDELDRFAA
                     PTRTVIEWS"
     gene            complement(4255..5190)
                     /locus_tag="VFU75_01920"
     CDS             complement(4255..5190)
                     /locus_tag="VFU75_01920"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5465612.1"
                     /translation="MRLPALWVTLALPAGVAAQMWQPAPGHSQIALWPGTAPRTRPSP
                     DSESVGTASSVAGRPWHYVSRVSRPTLTVYSPAGENTGAAVLVFPGGGYQILAIDLEG
                     TEICDWLTSRGITCVLLKYRVPRSGPSWVDHRHINPKPAPALQDAQRAMGIVRQHAAE
                     WHLDPHKVGVLGFSAGGHLVAAISNDYQERSYAPVDDADQLSCRPDFAVAVYPGHLWI
                     PDHRIPLKFNPDIHVTIHTPPTMLIQAETDSVDNVNQSLVYYIALKDAGVPVEMHLYT
                     NGGHAFGLRRTKDPITEWPQLVERWLGTIGILSGH"
     gene            complement(5187..5627)
                     /locus_tag="VFU75_01925"
     CDS             complement(5187..5627)
                     /locus_tag="VFU75_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465613.1"
                     /translation="MNVRPLLLTALIAVAPASSPDLSRLAGDWVLQFRAFPATPATQA
                     KRGVATLTLADSVDHGRHAMMGPYSLSMGGLTRPDPCISTGGTARLVPMRRDSVRVDL
                     RQGFNCGLWIVGVVRGDSVLGKWRQKSPAGIVAQGRFVMTRARR"
     gene            complement(5624..6073)
                     /locus_tag="VFU75_01930"
     CDS             complement(5624..6073)
                     /locus_tag="VFU75_01930"
                     /inference="COORDINATES: protein motif:HMM:NF015004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine dimer DNA glycosylase/endonuclease V"
                     /protein_id="HEU5465614.1"
                     /translation="MRLWSLHPRHLDPKGLVALWREALLAKAVLQHRTRGYRKHPQLD
                     RFRAHESPVAAINTYLAAVYAEACRRGYAFDGRRFRGPRTTRRIRVTRGQLAFEWGHL
                     RRKLRSRAPSAYRATAGSRPTAHPLFRVAAGPVADWERGGSRRIMEA"
     gene            complement(6070..>6663)
                     /locus_tag="VFU75_01935"
     CDS             complement(6070..>6663)
                     /locus_tag="VFU75_01935"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU5465615.1"
                     /translation="PDQSLKHPLGTTGPELMEWFFPTRVWREMQGGQGGEEGVDNDFA
                     AKGFENIGAHILGRNMFGPVRGPWPDESWRGWWGEEPPYHHPVYVLTHHARPSLVMKG
                     GTVFHFVTDGIRSALEQAKQAAGGKDVRLGGGAATIRQYLAAGLVDDLHLAVRPVVLG
                     KGEPLFAGLDLRALGYECTGLTAGERAAHVLIRRRVA"
BASE COUNT         1140 a         2236 c         2221 g         1066 t
ORIGIN      
        1 ggcacgagcg gcggagatgg catcatcggt cattctcctc gggtgtgggt cacttggctt
       61 ccaggtattc ccgcagggcc gacgcgttgt tgagacgcgt ctccctcgag ctgaatagca
      121 gtgtcacggt gcccttcccc atctccgtct tgagctcggc gaccccctca ggattcgcat
      181 cgagctcggc aaagtatcgc ttccgaaatt ccgcccactt ctccggatcg tgcccgtacc
      241 atttccggag cgcggtcgac ggagcgatcg tcttcgccca atagtcgatt cttgccttcc
      301 ctttcgacag tccgcgggcc cagagccggt ctatcaggat ccggcggcca tcgccggtgg
      361 cgggcggatc gtacacccgc ttggttcgga tcttcacgac cccggcttcc ttcccacgac
      421 gcagatccag cctttccagc gctccacgga aatctccacc tcggcgaacc ccgccgccgt
      481 caatgcttca cgatgctggt cgaccgtcaa gtacttggca cggagcagca tcatcggcaa
      541 gcggagcagg agtccgaacg cctggccccg gtacgtctcg gcgatgatga ccagcgaccg
      601 gcccggcttg aggacgcgcc tgacctcccg gagatcggcg acgagatcgg gccagtagta
      661 atgggtttcg acggcggtca cgaggtcgaa cgccgcgtcc gcatagggga ggtgggaaac
      721 cgaggcctcc tggatgtcca cccggcccgc ggcgatttcc gcctgattga cccgccgcgc
      781 ttccgcgacc gaggcgtcgg agtagtcgat ccccgagacg tgcccgtggg gagcgaggcc
      841 ggcgagcttc tggaccgtcc gtccacctcc gcatccgacg tccagtatct ggaacgacgg
      901 ctcgatccgc gtgtggccga gcccccagtc ggttacggtc gaatgcctga ggttcatggc
      961 ctggagcacg cgctggccca tggccccgct gggcttccgg cactgattca tgatgaagac
     1021 cacgagccct ccggactcag tgcgttcggc gcggcctcgg caccagctcg cctccgcaat
     1081 tcgggcagat cgatttcatg gcggacgtgc acgttgggca aaaactgcat tcgtacgtgc
     1141 aaatgtatgc cacgccatcg ggaggaagcg cggcaccgca ccgctcgcag gtcgcacgca
     1201 tctcgagggc catcaatccc ccggcggtgg ttgctgggcg aggtccacgt taccggaccg
     1261 ggagcgaaaa caccgcgcca tgtggtgccg cccggccggt gtacccgtgc ggccaggtcc
     1321 gatggctgag gtcgagccca ggcaccgacg cgccgggatc cttgaattcc gaatccaccg
     1381 acagcgtgcc cgtcgcggga tcgaagttca cgatgaagag gcggtccccc ttggcgtacc
     1441 cacccgaatt cacgaccagg cggcgacccg tgggatcgat cgccatccaa tggggtgact
     1501 cgtcgtctcc cagcgtcgcc tcggacactt ccctcggatg ctcggcatcc gagacgtcga
     1561 gcgccacgag cttgtgctcc gacgacacgg tctggatcca ccagtgcccg gtgagaatcg
     1621 gcacgccaca cggcgttccc gcaaaggtct tcaccagttc ggcgctcggc gccccggtct
     1681 cgacgccgcg gagcagatag aggccgcacc ggaacgtgtg gatgtagacg ctgtgtccgt
     1741 cagggagcag gaaaggctct ccggtaagct gattctcctt gccagcggga ccggggggca
     1801 gtgcgatgga tttctgcaac ttcaggtccg acagccgcca cagttgcacc cactggttcg
     1861 cggccgtgtc ggtcaggtcc atgtcagtcg tggtggacac cgcgacgttc agcgagggga
     1921 tcgggagcac gctgtatggc gaaatgagcc gctcggagac cgcggagtcg cgtgccgagc
     1981 cggtcgcgag gacatggccg cgctcgtcca tgtgcaccag gccgcccgtc gtgttcgggc
     2041 ccatggacat gccgccggca cccgccgcgt actggaacgt cgcgagaatc gtgttgtcgg
     2101 cgagccggac gtaggtgtgc gggtggctga acccgccaag atcaccgaac gacgtgagga
     2161 tgcgcggcgc ggccggtttc gtgaggtcga agagccaggt cttcccggcg tggaaaccgt
     2221 tggccaggag gtggccgtcg gccggcatca cggcttcggt gtggtggggg tgggtacccg
     2281 ccgccccgac cgccagagat gtcaccaccg atccgtagtg cggggacccc ggatcggcgt
     2341 cgatcaccgc caggaaatcg gatgcggtgt cgttggcgcc ggcccagacg aagaggtaag
     2401 acgagggcgc cggcgcgcgg aaggccgcga cggccacccc gacgacaacg gcggcgaaga
     2461 cgagcgggat gatctttttc atggttagac gttcgccttc gccggcacgt ccggcaactg
     2521 gccgcagtgc tacttcccga tgatcagcag ggccccggct gccaccgcga gtatcgacat
     2581 gatgaggctc agtcctgaaa agctgagatg aatcatcgag atcaacccgt tgagaatcag
     2641 ccacgctcca agcagaaaga acgcgagatt tctcgagagc ttcatggttc gctcctggtg
     2701 aaagtgtcag tccttcggtg aggccttgag ggcggcagcg gcacccatga cgaccagcgc
     2761 cgtcgagatg accgaacctc cgatgccgac cccgttcgca atgaatgcga tcatgaccat
     2821 gggtgaggag ctggggccga acgcgtgctg cccaagaatg ccctgcagca ctgtcccgac
     2881 cgagagccca tatccggtgg cgagcagggc ccagaacagt cccaaggcct cccgcttcgc
     2941 gagcaccagc tgggacatca ccgccgacac cgccagcatc agcgttccgg taaggatcag
     3001 tgcctcgtgc gcggtgtgcc agtagcgttc cgcgtccgac tccaagaccg tggggaagcc
     3061 acagaagagc ccgataagca tcaccacggc cccgtggagc gtgacacgca gccggttcgt
     3121 gcgatccatc cgggctacct cctcttcgcg ggttcgcggg agtcccgcag ctcgacgagc
     3181 gtgcgggaga gcgacgcgtg agcctgcgtc aggaccactc gatcaccgtc cgcgtcgggg
     3241 cggcgaaacg gtccagttcg tcgaggattt cgttgacgac gcgagtgtcg ggcgtcacgc
     3301 gacgctccac cacgcgctcg aggtccacgg agcccttctc gatgaggtcc aggagagccg
     3361 gcagctcgga cttcaggtgg tcattggcgc cgaggagctc ggcttcgcgc ccgagcaagt
     3421 cgcgataggt gtccaccgac attggcttcg tcgcgagacc cgccacgacc gcccggccat
     3481 gcaccgcgag gaccttcaag gactgttcca tggtgagcgg gagtccgatg agctcgaggg
     3541 cgacgtcgac gcctcgaccg ccggtatgct cccgaatctt cccaaccgga tctcccaccg
     3601 cggcgcggat ggtcacggcc ccgaacgatt ccgccagctt gagcttgcgc tcgtccagat
     3661 ccaccgcgaa cacctccgcg gcgcccaggg cgcgggccag ctgcaccgca gacatgccga
     3721 ggccgccgat cccgaagacc gcgactcggt ctcccttcgc gaggcggccc cgcttgagcg
     3781 cgtgaaacgc cgtggccgcg gagcacatcg cgacggcgcc atgcgcaaac ggaatccccg
     3841 ccgggagcgg caccgcgttg accgcgggaa cggcgatgaa ctccgcccac ccacccgccc
     3901 gatgatgccc gatcatcgcc ccggtggcgc agaactgctc atcgccccga acgcacgcgg
     3961 ggcacccgcc gcagctgatc agatagtgga cgcagacccg gtctccgggc tgcggcgcct
     4021 tcacctcgac cccgacccgc tcgaccaccc ccgcgacttc gtgaccgagc gtgaggggga
     4081 gaggccccgc cggtgaccgc ccggcgcgat agtgcgaatc ggagtggcag atgccggcgg
     4141 catggacgcg aatcaggacg tcattcggac cgaaggcggg ttcagggacg tcgcggagcg
     4201 cgaccggcgc gccgattgca accagctgaa gagctttcac gcaactctcc cagctcagtg
     4261 acctgacagg ataccgatgg tccccagcca tctctccacc agctggggcc attcggtgat
     4321 cgggtccttg gtgcgccgga gcccgaaggc gtgcccgcca ttcgtgtaca agtgcatttc
     4381 caccggaaca ccggcgtcct tcagcgcgat gtaatacacg agcgactgat tcacattgtc
     4441 cacgctgtcc gtctcggcct ggatcagcat cgtcggcggc gtgtggatgg tgacgtgaat
     4501 gtcggggttg aacttcaggg ggatccggtg gtccggaatc cagagatgcc ccggatacac
     4561 ggcgacggcg aaatccggac ggcagctgag ctggtcggcg tcatccaccg gggcgtacga
     4621 gcgctcttgg tagtcgttgc tgatcgccgc gacgaggtgc ccacccgctg agaagccgag
     4681 gactccgacc ttgtgcggat cgaggtgcca ttcggcggca tgttggcgca cgatccccat
     4741 cgcgcgctgc gcgtcctgca gggccggcgc cggcttggga ttgatatgac ggtgatccac
     4801 ccacgacggc ccggaacggg gcacccggta cttgaggagc acgcaggtga tgccgcggga
     4861 cgtcagccaa tcgcagatct cggtcccttc gaggtcgatc gcgaggatct gatagccgcc
     4921 gcccggaaat acaaggaccg cagcaccggt gttctcccct gccggtgaat agaccgtcag
     4981 ggttgggcgc gaaactctgg aaacgtagtg ccagggcctg ccggcgacgc tcgacgccgt
     5041 gccgacggat tcgctatccg gactgggtcg ggttcggggc gcggtgcccg gccacagggc
     5101 gatctgactg tgtcccggcg ccggttgcca catctgggcc gcaacgccgg ccggaagtgc
     5161 gagcgtgacc cacagagccg gcaacctcac cggcgagccc gggtcatgac gaatcggcct
     5221 tgggccacga tgccggcggg cgacttctga cgccattttc ccagtactga atccccacgc
     5281 accacgccga cgatccacag tccgcaattg aatccctgcc gcaggtccac ccgcacggag
     5341 tctcttcgca tgggcaccag ccgggccgtg ccaccggtcg agatgcaggg atcgggtcgc
     5401 gtgaggccac ccatggagag gctgtacggt cccatcatgg cgtgccggcc gtggtccacg
     5461 ctgtccgcga gagtgagcgt ggccactccg cgcttcgcct gcgtcgctgg tgtcgcgggg
     5521 aaggctcgga actgaaggac ccagtcaccc gcgagacgcg acaggtccgg ggaagatgcc
     5581 ggcgcgacgg cgatcagcgc cgtgagcaag agcggccgta cgttcacgcc tccatgatac
     5641 gacgcgaacc ccctcgttcc cagtccgcga cgggtccggc agcgacgcga aacagggggt
     5701 gagcggtcgg tcgcgagccg gcggtcgcgc ggtaggccga cggagcgcgg gaacgaagtt
     5761 tccgccgcaa atgaccccat tcgaaggcga gttgccctcg cgtcaccctg atccgccggg
     5821 tagtccgggg cccgcggaat cttcgaccgt cgaacgcata gccccgccgg caggcctcgg
     5881 cgtatacggc ggcgagatag gtattgatgg cggccaccgg ggactcgtga gcgcggaagc
     5941 gatcgagctg agggtgcttc cggtagccgc gcgtgcggtg ctggaggacg gccttggcga
     6001 gcaacgcctc acgccagagc gccaccagtc ccttcgggtc caggtgccgc gggtggaggg
     6061 accagagcct cacgccacgc ggcgcctgat cagcacatgg gcggcgcgct ctcccgcggt
     6121 caggcccgtg cactcgtacc cgagtgcgcg gagatcgagt ccggcgaaga gcggctcgcc
     6181 cttcccgagg acgacggggc gcaccgcgag atgcaagtcg tcgacgagtc cggcggcgag
     6241 gtattgccgg atggtcgccg cccccccacc gagtcgtacg tccttcccgc cggcggcctg
     6301 cttggcctgc tcgagggcgg agcggatacc gtcggtgacg aagtggaaca cggtgccgcc
     6361 tttcattact agagacggcc gtgcatggtg cgtgaggacg tacaccgggt ggtgataggg
     6421 tggctcttcg ccccaccacc cgcgccagct ctcgtcgggc cacggtcccc gcaccgggcc
     6481 gaacatgttg cggcccagga tgtgcgcccc gatgttctcg aatcccttcg ccgcgaagtc
     6541 gttgtcgacg ccctcttccc cgccctgtcc gccctgcatt tcccgccaca cgcgggtggg
     6601 gaagaaccac tccatcagct cgggccccgt ggtgccgagg gggtgcttga ggctctggtc
     6661 ggg
//
LOCUS       DASUBP010000042         7558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_600682, whole genome shotgun sequence.
ACCESSION   DASUBP010000042 DASUBP010000000
VERSION     DASUBP010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7558
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_600682"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..381)
                     /locus_tag="VFU75_01940"
     CDS             complement(<1..381)
                     /locus_tag="VFU75_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465616.1"
                     /translation="MHPLPLLPTPSNRLAVLALLLSLAGGSLAAQGISQGFELERAGK
                     LPQAVDYYRTVLRADSTNMVALLGLERVLPQLGRLNELVPLVERAAHATQERDAGVLG
                     LELRTFVALSMNDSVAALAHRWTAR"
     gene            complement(347..3619)
                     /locus_tag="VFU75_01945"
     CDS             complement(347..3619)
                     /locus_tag="VFU75_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465617.1"
                     /translation="MTETARRVARLAAPHARGRALAAFIVAVGMGLVAAALGVFLSPN
                     VWGVVLGWALVVGAACSGWLIWRQETRALGPHHVGTLVELLGRGRLGSVVNLLQAPPA
                     PGTGLWSAADARAAAQVDRAAPEVGDHLRHRTRRFYVGGLAVVLAGASLLLAARPRSA
                     HADAFWHPLRTINNARAPVRLTLDRVTARRGDTVHATITVLGGTTARLWTRAPGEPWS
                     EATVVLDSSGTAHRALGPLSSDLFVRVTNGSRSSPTLAIHIPPSAFVGALTVTAHYPR
                     YLERADETLPIGNDTAVIPEGTVLTAEGEASVPLRSARWSPGSPLDVQGAQFRGSLRP
                     TNGGRYSLLITPRDSSPVEGEPPVIAIKVVPDSAPVVALTLPAADTTLPPTLHQPVVI
                     DAHDDHTLTRVTLQSWRASQTGKIGDTVSQPLTLDAVTDRAILQADLDASVRGLLPGD
                     TLKLRVVAWDNAPRAHEGHSATIALRLPTLAELRAATRAATAELGSTTDSVLNAARSL
                     SAETRDLAAERTRAGTTQRTGSEQPGGDPKAGAMPFESSQRAQELARRQEELSQKVQD
                     LSRAVQDLAQAAHAAGIDDSAFQRRLADVQQLLQKAITPELEQRLRELQDALQRLDPE
                     ATRQALQHLADAQNELKDALQRSDQLFRRAAVEGQLATLTADAEELKRRQGDWNSTQA
                     PRADSAATAQERELAAGADSLMKGIQQAGQDLRSGQTLDQPMQSAQQARQAMGRAGDA
                     AGQGNAAEAKQQGQMAEQALKDLPEQLKSRRDSLSGAWRQETLDALDRAMSETADLAR
                     RQQQIADQLNQGQSGAPIRSAQASVEEGAQAVSQQMQETAGKNALVTPQLSAVMALAR
                     RQMAQARQEIEQARPNTQSASQLAAQAVDALNAAALGISEARAQVEGAKSGSGLAEAL
                     EQLSKMAGQQQGLNGESQGLLPMMGAGGAVNEQLRALAARQRALADQLQRLQAGGNAP
                     GAGAMADEAKEIARRMEAGQLDPVTVARQQSLYHHLLDAGRSLTGDEPDDQHERRAPI
                     AGTVNPLLPPALRPGATGTGARVPYPTWESLRDLTPGQRQQVLEYFRLLNAPAPAAPH
                     AQ"
     gene            complement(3616..4275)
                     /locus_tag="VFU75_01950"
     CDS             complement(3616..4275)
                     /locus_tag="VFU75_01950"
                     /inference="COORDINATES: protein motif:HMM:NF025086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HEU5465618.1"
                     /translation="MKLAAAGLLAAVLSAGATPAPVLTIGRLHYDGGGDWYSGPSALP
                     NLLAAIRQRTGLKVTDRERVVTLASPDLWDIPYLFVTGHGNLHFTDAEVKILRRYLEG
                     GGFLHADDDYGMDASFRREIARIFPDHPLVDVPLTHPVYHIVYDLPNGIPKIMEHDGL
                     PARGLGIFLDGRLVVYYSYQTDLNDGWEDFDVHHVPPEKHEQALRMGVNLFAYAVSGI
                     K"
     gene            complement(4272..5108)
                     /gene="rsmI"
                     /locus_tag="VFU75_01955"
     CDS             complement(4272..5108)
                     /gene="rsmI"
                     /locus_tag="VFU75_01955"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEU5465619.1"
                     /translation="MAGTLYVVATPLGNLGDLSPRAAQTLKSVALVAAEDTRHSRPLL
                     THIGSTADLVSFHAHSSDGDLRRLLERLAAGQDVALITDAGTPLVSDPGVSLVAGARE
                     RGIPVVTIPGPNAVVAALAVAGLPADRYLFLGFLPRKGGDRKRLLASVAESPWTVVLF
                     ESPLRVATLLEDLAEVCGGDRRAAVARELTKVFEETRAGTLADLAGYYGDAPARGEVT
                     VVVAGTGRDPGTKVEAPLPDPEVRARELLAEGLSRRDVADRLAEETGINRKTAYRLVN
                     EL"
     gene            complement(5119..6135)
                     /locus_tag="VFU75_01960"
     CDS             complement(5119..6135)
                     /locus_tag="VFU75_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465620.1"
                     /translation="MRASTARLTNSPVRLADLRFPQVDQLIHRTRLAFIHLDNLMGYA
                     KRDRDGRVDGYICAHVPDGCLLLFFRKGEPVNAAAFEVSGREVITITEVLRRMRAETE
                     RGDLTYCAAPMEQLAWMYQSCAVPQQPRLVDPSQPAALFPVLREEKVTGVLEFISNGR
                     VSYVRLDEGKFVSGYFCEKPADMPVPRYFESLFKPLPDGSDAALAACVFPYVADLPAQ
                     APHALINTYRELYWRIVDAVEQEFPGEGKKRAQRLSSTHQRDHKALTSIGVPRGTEIP
                     DAVVRPDELSTALAIWGKELLDSVEIMMPGTAVKVLRGATREHRYVLQSAGFYDALPW
                     SVTW"
     gene            complement(6154..6480)
                     /gene="trxA"
                     /locus_tag="VFU75_01965"
     CDS             complement(6154..6480)
                     /gene="trxA"
                     /locus_tag="VFU75_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEU5465621.1"
                     /translation="MSDLTQVTDANFATEVEQQKGLVVVDFWATWCGPCRMVAPIMEQ
                     IAGQYAGKVKVTKLDVDANQKTAMRFNVRSIPSILFFKDGAHVDTLVGAYPKPVFDEK
                     ITRHIG"
     gene            6521..6916
                     /gene="mce"
                     /locus_tag="VFU75_01970"
     CDS             6521..6916
                     /gene="mce"
                     /locus_tag="VFU75_01970"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HEU5465622.1"
                     /translation="MDDTNRIAHVGVAVRDLAEASRFYREVLGLSPSAPETADGAEIV
                     HFHFGGVDVELLAARDPRSPIAAFLEKRGPGIHHICYRVPDLDQALARCREAGYRLVD
                     EVPRQGAGGKRIAFVHPKATAGILLELTE"
     gene            complement(6943..7317)
                     /locus_tag="VFU75_01975"
     CDS             complement(6943..7317)
                     /locus_tag="VFU75_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465623.1"
                     /translation="MPEPKSPNVLLSRFLDAVKAKDTRTMAALWGTADGPWRKVPDAY
                     RDSVMTSFQIYLRHDTYKVLDGPLPSSSDSRVMTFHVELEAPQCTRSQPFDVIKTKGG
                     GWLMFNVHLEAEGSVIPYCPRP"
     gene            7431..>7558
                     /locus_tag="VFU75_01980"
     CDS             7431..>7558
                     /locus_tag="VFU75_01980"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF015685.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEU5465624.1"
                     /translation="MRLYVNIDHVATIREARKTDEPDPVRAAADALAGGADGITVHL"
BASE COUNT         1166 a         2616 c         2589 g         1187 t
ORIGIN      
        1 ccgcgcggtc cagcgatgcg ccagagcggc gacgctgtcg ttcatgctga gcgcgacgaa
       61 ggtccgcagc tcgagtccca ggaccccggc atcgcgctcc tgggtggcgt gcgccgcccg
      121 ctcgacgagg ggcacgagct cgttcaatct cccaagctgt ggcagcaccc gctcgagtcc
      181 gagaagcgcc accatgttgg tcgagtccgc gcggagcacc gtgcgatagt agtccaccgc
      241 ctgggggagc ttgcccgccc gctcgagctc gaacccctgg ctgatcccct gcgcggccag
      301 tgatccgccg gcaaggctca gcaggagcgc gaggaccgcg agccggttac tgggcgtggg
      361 gagcagcggg agcgggtgca ttgagtagac ggaaatactc gaggacctgt tgccgttggc
      421 cgggtgtcag atcgcgcaag gactcccacg tgggatacgg tacgcgcgcg ccggttccgg
      481 tcgcgccggg gcgcaacgcg ggaggcagga gcgggttgac ggtaccggcg atcggtgcgc
      541 gccgctcatg ctggtcgtcg ggttcgtcgc cggtgagcga ccgccccgcg tcgagcagat
      601 gatgatagag cgactgctgg cgcgcgaccg tgacgggatc gagctgcccc gcctccatcc
      661 gccgcgcgat ctccttcgcc tcgtccgcca tcgctcccgc gcccggcgcg ttcccgcccg
      721 cctgcaggcg ctgcagctga tcggcgagcg cccgctggcg cgcggcgagc gcccgcagct
      781 gctcgttgac ggccccaccc gctcccatca tcggcagcaa cccttgcgac tctccgttca
      841 gcccctgctg ctgccccgcc atcttcgaga gctgctcgag ggcctcggcc aggccgcttc
      901 ccgacttcgc accctccacc tgcgcccgcg cctccgaaat gccgagggcg gccgcattca
      961 aggcgtccac cgcctgggcg gcgagctgcg acgcgctttg cgtgttggga cgcgcctgct
     1021 cgatctcctg gcgggcctgc gccatctgcc gcctggcgag cgccatcacc gcggaaagct
     1081 gaggagtgac gagggcgttc ttccccgccg tctcctgcat ctgctgagag accgcctgcg
     1141 caccctcttc caccgaggcc tgcgccgacc ggatgggcgc gccgctctgc ccctggttca
     1201 gctggtcggc gatctgctgc tggcgccggg ccaggtccgc cgtctcgctc atggcacgat
     1261 ccagtgcgtc cagcgtctcc tggcgccacg cgccagagag cgaatcccgc cgcgacttga
     1321 gctgttccgg cagatccttc aaggcctgct cggccatttg cccctgctgc ttcgcctcgg
     1381 ccgcattccc ctgacccgcg gcgtccccgg cccggcccat ggcctgccgc gcctgttgcg
     1441 ccgactgcat cggctggtcg agcgtctgcc ccgagcgaag gtcctggcct gcctgctgaa
     1501 tccctttcat cagggagtcg gccccagcgg cgagctcgcg ctcctgcgcc gtcgcggccg
     1561 aatcggcccg cggggcctgc gtcgagttcc agtccccctg ccgccgcttc aactcttccg
     1621 cgtcggcggt cagcgtcgcc agctgtccct cgacggccgc acgacggaag agctgatcac
     1681 tccgctgcag cgcgtccttc agctcgttct gcgcgtccgc gagatgttgc agggcctggc
     1741 gggtcgcctc ggggtcgagc cgctggagtg cgtcctgaag ctcgcggagc cgctgctcca
     1801 gctccggggt gatcgccttc tggagcagct gctggacatc ggccagccgc cgctggaacg
     1861 ccgaatcgtc gatccccgcg gcgtgggcgg cctgcgccag gtcctgcacg gcgcgggaga
     1921 ggtcctgcac cttctggctc aactcctcct ggcggcgggc gagctcctgc gcccgctgcg
     1981 acgactcgaa tggcatcgcg ccggctttgg ggtcgccgcc cggctgctca ctgccggtcc
     2041 gctgcgtcgt gcccgcccgc gtccgctcgg ccgccaggtc ccgcgtttcc gcgctgaggc
     2101 tcctggctgc gttcagtacg gagtccgtcg tggagccgag ctcggcggtc gccgcacgcg
     2161 tcgcggcgcg gagctccgcc aaggtcggca gccgcagcgc gatcgtggcg ctgtgcccct
     2221 cgtgcgcccg cggagcattg tcccaggcca cgacgcgaag cttgagcgtg tcccccggga
     2281 gcaagccccg gacggaagcg tcgagatcgg cctgcaggat cgcgcggtcg gtgaccgcgt
     2341 cgagcgtgag gggctgagag accgtgtcac cgatcttgcc ggtctgactc gcgcgccagc
     2401 tctggagcgt gacccgcgtc agggtgtgat cgtcatgcgc atcgatcacc accggctgat
     2461 ggagcgtagg tggcaacgtt gtatccgcgg ccgggagggt gagcgcgacg accggtgcgg
     2521 agtcgggcac caccttgatc gcgatgacgg gaggctcccc ttcgacggga gacgaatctc
     2581 gcggcgtaat gagcagggag taccggccac cgttcgtcgg ccggagggag cccctgaatt
     2641 gcgcgccctg cacgtcgagc ggcgacccgg gagaccagcg cgccgacctc agcggcacgc
     2701 tcgcctcccc ctcggcggtc agaacggtcc cctcaggaat gacggcggtg tcgttcccga
     2761 tcggcagggt ctcatccgcc cgctccagat acctggggta gtgcgcagtg accgtgagcg
     2821 cgccgacgaa cgcggacggc ggaatgtgga tggcgagcgt cggcgacgag cgcgagccgt
     2881 tggtcacgcg aacgaacagg tcggagctca ggggaccaag tgcacggtgc gccgtcccgc
     2941 tcgagtccag caccaccgtc gcctccgacc acggttcgcc gggagcgcgc gtccacaagc
     3001 gcgcggtggt ccctccgaga accgtgatcg tcgcgtgcac cgtgtcgcca cggcgagcgg
     3061 tgacgcgatc gagcgtgagc cgaacgggcg cacgggcgtt gttgatggtg cggagcgggt
     3121 gccagaacgc atccgcatgg gccgatcgtg gtcgagccgc cagcaggagg gaggcaccgg
     3181 cgagaacgac cgccaatcct cccacataga agcggcgggt ccggtgcctg aggtggtcac
     3241 ccacttcggg ggcggcgcgg tccacttgcg ccgcggcccg cgcatccgcg gcgctccaca
     3301 gtcccgtacc gggcgcgggt ggcgcctgga ggaggttgac gacacttccc aaccggccgc
     3361 gaccgagcaa ctcgacgagc gtgccgacgt ggtggggacc gagcgcgcgc gtttcctggc
     3421 gccagatcag ccagccggag caggccgcgc cgacgaccag ggcccagccg agcacgacac
     3481 cccaaacatt gggtgagaga aagacgccga gcgccgccgc gaccagtccc atgccgacgg
     3541 cgacgatgaa ggcggcgagc gctctgcccc gcgcgtgcgg cgccgcgagg cgcgccacgc
     3601 gacgggcggt ctcggtcact tgatcccgct gacggcgtag gcgaagagat tgacgcccat
     3661 gcgcagtgcc tgctcgtgct tttcgggggg tacgtgatgc acgtcgaagt cctcccaccc
     3721 gtcgttcagg tcggtctgat aggaatagta cacgaccagc cgtccgtcca agaagatgcc
     3781 caggccccgc gcggggagcc cgtcatgctc catgatcttc gggatcccgt tcggcagatc
     3841 gtacacgatg tggtacaccg gatgggtcaa gggaacgtca accagggggt ggtcggggaa
     3901 gatccgggcg atctcccggc ggaaggacgc atccatcccg tagtcgtcat cggcgtgcag
     3961 aaacccgccc ccctccagat accgcctgag gatcttcacc tcggcgtccg tgaagtggag
     4021 gttgccgtgc cccgtcacga acagatatgg gatgtcccac agatcggggc ttgccagggt
     4081 gaccacgcgc tcccgatcgg tcaccttgag accggtacgc tggcgaatgg cggcaaggag
     4141 gttcgggagg gccgacgggc cggagtacca gtccccgccc ccatcgtagt ggaggcgtcc
     4201 aatcgtgagg acaggcgcag gggtggcacc cgccgagagg acggcggcga ggagtccggc
     4261 ggcagcgagc ttcacaattc gttgaccagg cggtaggcgg tcttgcggtt gattcccgtt
     4321 tcctcggcga gccgatcggc cacatcccgg cgcgacagcc cctcggccag gagctcgcgc
     4381 gcgcgtactt cgggatcggg cagcggcgct tccactttgg tgcccgggtc ccgccccgtt
     4441 ccggcgacca ccaccgtcac ttctcctcgc gcgggcgcat cgccgtagta acccgcgagg
     4501 tcggccaggg tccccgcgcg cgtctcctcg aacaccttcg tcagctcccg ggcgaccgcc
     4561 gcccggcggt cgccgccgca cacctcggcc aggtcttcga gcagcgtggc gacgcgcagc
     4621 ggcgattcga acagcaccac cgtccacggg ctctcggcga cgctcgcgag cagccgcttc
     4681 cggtccccac ccttccgggg caggaagccc aggaacaggt atcggtcggc cggcaggccg
     4741 gcgacggcga gcgcggcgac gaccgcattg gggcccggga tggtgacgac gggaatcccg
     4801 cgctcccgcg cgccggcgac gaggctcacg ccggggtccg agacgagtgg agtgccggcg
     4861 tcggtgatga gggcgacgtc ctgacccgcg gcgagccgct cgaggaggcg gcggaggtca
     4921 ccgtcgctcg agtgtgcgtg aaacgagacc aggtcggcgg tggagccgat gtgggtcaac
     4981 aggggacggc tgtggcgggt gtcttcagcc gcgaccagcg cgaccgactt cagggtctgt
     5041 gcggcccggg gcgagaggtc gccgaggttc ccgagaggcg tcgcgacgac gtagagcgta
     5101 ccggccacga cggcctcgtc accaggtgac cgaccagggg agcgcgtcat agaagccggc
     5161 ggactgcagc acgtaccggt gctcgcgcgt cgctccgcgc agcactttca ccgcggtgcc
     5221 cggcatcatg atctcgaccg agtcgagcag ctccttcccc cagatcgcga gcgccgtcga
     5281 cagctcgtcg ggacggacca cggcatccgg aatctcggtg ccccgcggca cgccgatgct
     5341 ggtcagcgcc ttgtgatcgc gttggtgcgt ggacgagagg cgttgcgcgc gcttcttccc
     5401 ttcccccggg aattcctgct cgacggcgtc cacgatccgc cagtacagct cgcggtacgt
     5461 attgatgagg gcgtgcggcg cctgcgcggg caaatcggcg acgtacggaa acacgcatgc
     5521 cgcgagcgcc gcgtcggacc cgtcgggcag cggcttgaag agtgattcga aatagcgcgg
     5581 caccggcatg tcggccggct tctcgcagaa gtagccgctc acgaacttgc cctcgtccaa
     5641 ccggacgtag gacacgcgcc cgttggaaat gaactcgagc acgcccgtca ctttttcttc
     5701 tctgagaacc ggaaagagcg cggcgggctg gctgggatcc acgagccgcg gctgctgggg
     5761 cacggcgcac gactggtaca tccacgccag ctgctccatg ggtgcggcgc agtaggtcag
     5821 gtcgccgcgc tcggtctcgg cgcgcatgcg gcgcaggact tcggtgatgg tgatgacttc
     5881 gcgccccgac acttcgaagg cggcggcgtt caccggctcg cccttccgga agaacaacag
     5941 caggcagccg tccggcacat gggcgcagat gtatccgtcg acgcggccgt cacggtcgcg
     6001 tttcgcatac cccatcaggt tgtcgaggtg aatgaacgcc aggcgggtgc ggtgaatcag
     6061 ctggtccacc tgcgggaagc ggaggtccgc gaggcgcacc ggcgaattcg tgagtcgggc
     6121 ggtgctggct cgcatggacc tccgcctgga tggttacccg atgtggcgcg tgatcttctc
     6181 gtcgaacacg ggcttgggat aggcaccgac cagggtgtct acgtgggccc cgtccttgaa
     6241 gaatagaata ctcggaatcg accgaacgtt aaagcgcatg gccgtcttct ggttggcgtc
     6301 cacgtccagc ttggtcactt tgactttccc cgcatactgg ccggcgatct gttccatgat
     6361 cggcgccacc atccggcagg gaccgcacca ggtagcccag aaatcgacga ccacgaggcc
     6421 cttctgctgt tcgacctcag tcgcgaagtt ggcgtccgtg acctgcgtca gatccgacat
     6481 tgctctccac tctcctcaaa accgttaagt tcttgtcgca atggacgaca ccaatcgcat
     6541 cgcccacgtg ggagtcgcgg tccgcgacct cgccgaggcc agccggttct accgggaggt
     6601 cctgggcctg agtccttcag cgccggaaac ggccgacggc gcggaaatcg tccacttcca
     6661 cttcgggggg gtcgacgtcg agttgctcgc cgcccgcgac ccgcggagtc ccatcgccgc
     6721 ttttctcgaa aagcggggtc ccggcattca tcatatctgc taccgggtcc ccgacctcga
     6781 tcaggccctg gcccgctgcc gggaggccgg atatcggctg gtggacgagg tcccccggca
     6841 aggtgccggg ggcaaacgca tcgccttcgt gcacccgaaa gctaccgcgg gcatcctgct
     6901 ggaactcacg gaatagaacg cccgccccac cgttcgagtt ccctacggcc gcgggcaata
     6961 cggaatgacg gatccttcag cttccagatg cacgttgaac atcagccagc cacccccctt
     7021 ggtcttgatg acgtcaaagg gctggctgcg ggtgcattgg ggcgcctcaa gctcgacgtg
     7081 aaacgtcatc acccggctgt ccgaactcga ggggaggggc ccgtccagaa ccttgtaggt
     7141 gtcgtgacgc aggtagatct gaaaggacgt catgacgctg tcacggtacg catcgggaac
     7201 cttgcgccac ggaccgtccg cggtgcccca aagggccgcc atggtgcgcg tatctttggc
     7261 tttcacggca tcgagaaacc ggctgagcaa cacgttcggc gacttcggct ccggcacttg
     7321 acccgcctgg taatgagaac aagcggcgcc ggcacagagc agtacaacga gtagagctcg
     7381 attcaagggg tggccccggt tggagggagc tgcaccgaag gtaagaactc atgcgcctct
     7441 acgtcaacat tgatcacgtc gcgaccatcc gcgaagcgcg gaaaacggac gagcccgatc
     7501 ccgtccgcgc cgcggccgac gcgctcgccg gcggcgccga cggcatcacc gtccacct
//
LOCUS       DASUBP010000043         8560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_300692, whole genome shotgun sequence.
ACCESSION   DASUBP010000043 DASUBP010000000
VERSION     DASUBP010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 8560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8560
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300692"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1556)
                     /locus_tag="VFU75_01985"
     CDS             complement(<1..1556)
                     /locus_tag="VFU75_01985"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5465625.1"
                     /translation="MKICPVCSTEFPDDQKFCPNDGSVLRGAAPTSDLVGQVVAERYH
                     VVKKLGEGGMGQVYLAEHVKMGRRSAIKVMNPSMTHDPDAVARFNREAANASRITHPN
                     VCAVYDFGETPDGLIYLAMEFIEGEPLTDLIAREGALKVGRAVKIFQQTADALQAAHD
                     LGIVHRDLKPDNIMLAKGRDGSDVVKVVDFGIAKAVGGDEAGQKVTKTGLVVGTPEFM
                     SPEQLSGDKLDGRSDLYSLALVLYRMLTGKLPFEATSVQETMIKRLTDEPTKLALARP
                     DLAFPPKLQGVLDRALARTPNERYQNVSEFAADVGKAIGATAAAPAMDTEGRTQVIGA
                     AEKTKIAEAVPATRVAAAGKEKKSLMVPVAVGIAVVALGGGAWAVFGRGPNTPPLSTQ
                     HDTTTHQVAANPGRDTTQMSHADTTKTPKNPGTRPANPGNKSPGPAGLDSATLAGVAE
                     RLLDMSPRAIIDSGQLVFNGAHVSPLDRSTSACAVALAYQQGGRRTEAVQWANKGLSI
                     DPTLSSCKNVL"
     gene            complement(1553..2668)
                     /locus_tag="VFU75_01990"
     CDS             complement(1553..2668)
                     /locus_tag="VFU75_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012708.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEU5465626.1"
                     /translation="MTSCPYCGRANDAAAAFCMDCGKPLTKSAAQAKARSSAAAGVSP
                     AGAPPGPISPATPLPHPSMTPAPNTRAPDPATPDAAAAPVQTGCPFCGKAVSGALPFC
                     PHCGRRLSAPGSGPACARCGSPVAFGVAFCATCGAPVSSTPALQRPSSQPPVKTDFTF
                     YLNLLDESGKAIQRYERRAMDTGIGRQDGDIRFPDDQFLSPLHARITWEQDRLMLRDL
                     GSRNGTWVFFDEPHKLVDGDLLLIGSQMIRFRRLGYPGPHPPDADATKRMGSLIPSAD
                     IASLTQLRSDGSARDVVQLSPGRDIRIGREEGDWVFPYDPSMSSKHAVVRSEDADFIV
                     IDDGSRNGIARAARGAVPLAGGSRILVGDKLLRIEFP"
     gene            2760..5543
                     /locus_tag="VFU75_01995"
     CDS             2760..5543
                     /locus_tag="VFU75_01995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465627.1"
                     /translation="MRSAGICALVVLALARSGSAQVEPSGVWRTLHTPHFRIHFRPSY
                     RDVALREAREAERAYGLLATELHPPRGSVDITLSDDFDGANGLTTVFPTDRVTLFLAP
                     PATDPALAQYDDWLRLVSQHELTHVFHLDRVRGVWGVMQTVFGRAPGLFPEEYQPSWV
                     TEGLAVYYESKFTTGGRVRGSYHSQVLAADAADSSSRPSSDALLFTRWADGYVPYAYG
                     SRFFRSIADALGDSVIPRFVERSAGQWIPYRVGHPLSGASPGVQLDSAWEADTRPAAG
                     PGTPGTVLERGLYSEPVPRVSPDRRWVVWLRDDGRGPRRLIMADARTWKESRSHYVNA
                     GVSYDWVGDTVMVAQVDLTGPHRIRSDLYRWDPDGSWTRLTRGARITEPRGGGGTLVS
                     VQVSPDGNHPTIPEVRDTAGTDWGDVVPSVDRRWVAATRHRDGHWQLVRWPWASPDSV
                     SVLSEGSSLVSGPTWWGDTLLFVSDVSGFPQVYRWNDSTGGPAARAVTAEPLGARSPA
                     ILPDGRIAYTSLRSTGWTLMSAPIRNGAALGPVPPAAPFDSAPAVPVRETGYTAWPSL
                     APHFWIPVYFDAGPSGRFGGAVTAGSDALGRWSYAAIGMASLSPVRFRGELSVVADLL
                     PQPVFDADVSNAWSNVGETSGGTVVSKLAQDADLGATFRYLRWRTAVQLRVAAEFERA
                     EYVATPDTALAAVCIGCASRDQIGGSVTLAVAHNSSGILSVSPQNGFNWSLTYRRRNQ
                     QGSPLFADEYRSLLALYAALPGPGFAPPVLALRFGVGETNGPLLRTLGVGGVSSGTYD
                     LGFGQTVGGVRDFPVRGYAGGALVDRRAATGTAELRIPLALVGRSLGHLPVGADKVSL
                     RPFADAGDAWAPGSSPRLTRLWSVGAELALDLTVNYDVPLALRLGLAEPMTAPPAGGP
                     RRAQVYVAFSSDF"
     gene            5648..7897
                     /locus_tag="VFU75_02000"
     CDS             5648..7897
                     /locus_tag="VFU75_02000"
                     /inference="COORDINATES: protein motif:HMM:TIGR04514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HEU5465628.1"
                     /translation="MRLLALLVVTATTVAAAQSPDQRAALERLRDSLETSSDSVALKG
                     LEAATIEVAKVNRDDPLIHLRLGFIAYRLGELAKESHHYDDAAGEFEWASQLKPDWPY
                     PWFGLGRSELAIGAHSSIAAENLKQFLHLDHLSKAINAYSKAAAVDPTFAEPVIELVE
                     TALRQRVRAETDVALRVARTAATSASGIPAFQMARGRIEREVGEGDSARAAFEAYLKD
                     GGDSGIGLLELARTDYYTHRDSIGHADYFAGARAAGSVAAIRMYRTDFSWIATPQELT
                     AFDSLRTSEARANWLKEFWAKRDVADVREPGSRLAEHYRRWFVAMRSFGLVSPHRHYD
                     FTEVYRSPQDLMDDRGVIYMRLGPADKVGAFSGSGGTAASGVEPNESWLYRRPGGDLI
                     FHFVSRGDVQDFKLVESLTDALSFQDRQRLQLSGDTMNVVRGLFASRSTFGPMYERIG
                     NTITSSNFLPAIAEDRQRGTYSIGVGTTTDDNHLRFDRPLDPAVEGYLVGASDSGGEQ
                     ELHLVFAIPAEELTPVGAASGVLYPLRFRLYVSTLSDSLVGRIDTLRVFGAPQILRSP
                     SYLTGQLEVPVPAGDYRYRLLIESKDGVTGAVASDTIAAASLDGRGFAMSDLVVGKTG
                     GLWWEQHGDTVYVNPLDRFPAGGDAQLYYEVYGLPVGGAYHTEIKVERKGGGSIFGAI
                     RRLFGGNHPPVQLEFDAAATGPVTRVHRTVALRDVSNGTYDLTLRITDPATGHVLTRE
                     RHFEVVSAN"
     gene            7953..>8560
                     /locus_tag="VFU75_02005"
     CDS             7953..>8560
                     /locus_tag="VFU75_02005"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5465629.1"
                     /translation="MYCSRCGTQNEDNAKFCKSCGLDLTATTPVHAISTGQQEVTEID
                     LVRDQLKEEYEILDELGRGGMAIVFKAREKQLERDVAIKVLPFSLAFDKEFVERFQRE
                     ARTSAKLEHPGIIPIYRVGKSGRVIYFVMKFLRGKPLSAVLSQRGALPPADIRRILTE
                     VGRALGYAHKSNIVHRDIKPDNIMFDDLGQAVVTDFGIAKAA"
BASE COUNT         1275 a         2896 c         2942 g         1447 t
ORIGIN      
        1 aggacgttct tgcacgatga cagggttggg tcaatggaaa ggcccttgtt ggcccactgc
       61 acggcttcag tgcgacgccc gccttgctgg taagcgaggg cgaccgcaca agccgacgtc
      121 gatcgatcga gcggggacac gtgtgcaccg ttgaagacga gctgacctga gtcgattatc
      181 gctcgtgggc tcatgtccag cagccgctcg gcgactcccg ccaaggtcgc ggaatcgagt
      241 cccgcggggc caggagactt gttgcccgga tttgccggtc gggtcccggg gttcttcggc
      301 gtcttcgtcg tatccgcgtg cgacatctgc gtcgtgtcgc gacccggatt cgccgctact
      361 tgatgagtcg tcgtgtcgtg ctgggtggac agcgggggag tgttggggcc tcggccgaat
      421 accgcccacg caccaccgcc gagcgccacc acggctatgc cgaccgcgac cgggaccatc
      481 aggctcttct tctctttgcc cgccgcggcc acgcgcgtcg ccgggaccgc ctccgcgatc
      541 ttggtcttct cggcggcccc gatcacctgc gtcctgcctt cggtgtccat cgccggcgcg
      601 gccgccgtgg ctccaatggc cttgccgacg tccgccgcga actccgagac gttctggtag
      661 cgctcgttcg gcgtccgcgc cagcgcacgg tcgagcactc cctgcagctt gggcgggaag
      721 gcgaggtcgg ggcgggcgag cgccagcttg gtgggctcat ccgtgagccg cttgatcatc
      781 gtctcctgca ccgacgtcgc ctcgaacggc agcttgccgg tcagcatccg gtaaagcacc
      841 agggccaggg agtagagatc actgcggccg tccagcttgt cgcccgacag ctgctcgggg
      901 ctcatgaact ccggcgtgcc cacgaccagg ccggttttcg tgaccttctg gcccgcctcg
      961 tcgcccccca ccgccttcgc gatgccgaag tccacgacct tcaccacgtc ggagccgtcc
     1021 cggcccttgg cgagcatgat gttgtcgggc ttgagatcgc ggtgcacgat gccgaggtcg
     1081 tgcgcggcct gcagggcgtc ggcggtctgc tggaagatct tcaccgcgcg cccgaccttc
     1141 agcgcgcctt cgcgcgcgat caggtcggtg agcggctctc cctcgatgaa ctccatcgcc
     1201 aggtagatga gcccgtcggg cgtctcaccg aaatcgtaga cggcgcagac gttcggatgg
     1261 gtgatgcggc tggcgttcgc cgcctcgcga ttgaaccgcg cgaccgcgtc ggggtcgtgc
     1321 gtcatcgagg gattcatgac cttgatggcg ctccggcgcc ccatcttcac gtgctcggcc
     1381 agatacacct ggcccatccc gccttcgccc agcttcttca cgacgtgata gcgctccgcg
     1441 accacctgac ccacgaggtc ggacgtcgga gcggcgccac gcagcacgct gccgtcgttg
     1501 gggcagaact tctggtcgtc ggggaattcg gtggagcaga cggggcaaat tttcacggga
     1561 attcgatccg caggagcttg tcgccgacca ggatgcgcga tccgccggcg agcggaacgg
     1621 ccccgcgcgc cgcgcgcgcg atgccgttgc ggctgccgtc gtcgatcacg atgaagtcgg
     1681 cgtcctcgga gcgaaccacc gcgtgtttgc tgctcatcga cggatcgtac gggaacaccc
     1741 agtcgccttc ctcgcgcccg atccggatgt cccggcccgg cgacaactgg accacgtccc
     1801 gcgcgctgcc gtcggagcgc agctgcgtca gggaggcgat gtcggccgag ggaatcaggg
     1861 agcccatccg cttggtggca tcggcgtcgg gaggatgggg gccggggtag ccgagccggc
     1921 ggaaccggat catctgcgag ccgatgagca ggaggtcgcc gtccaccagt ttgtggggct
     1981 cgtcgaaaaa gacccaggta ccgttccggc ttcccaggtc gcggagcatc aggcgatctt
     2041 gctcccaggt gatgcgcgcg tgcagcggag acagaaactg gtcgtcgggg aagcggatgt
     2101 cgccatcctg ccgcccgatg cccgtatcca tggctcgccg ctcgtagcgc tggatcgcct
     2161 tgccgctttc atcgagaaga ttcaggtaga acgtgaagtc agtcttgacg ggcggctggg
     2221 acgatgggcg ttgcagggcc ggcgtcgacg atacgggtgc cccgcaggtg gcacagaatg
     2281 cgacgccgaa cgccacgggt gacccgcatc gggcgcacgc gggtccggaa ccaggagccg
     2341 agaggcgccg gccgcagtgc ggacagaacg gaagcgctcc cgagacggcc ttgccgcaga
     2401 acggacatcc cgtctgaacc ggcgccgcgg cggcgtccgg tgtcgccggg tctggcgcgc
     2461 gggtattggg agcgggggtc atcgacggat gcgggagcgg tgtcgcgggc gaaatcgggc
     2521 cgggaggagc accggcgggc gagaccccgg cggcggcaga cgaccgcgcc ttggcctggg
     2581 ccgccgactt ggtcagcggt ttgccgcagt ccatgcaaaa ggcggctgcc gcgtcgttag
     2641 cgcggccgca gtaggggcag ctggtcaccg ggggcgctcc acaggacgct caagttaaga
     2701 tgcaccaacg gattcggcaa attggcgggc ccaaaccgcc cggcgtactt tgcgcgtcta
     2761 tgcgaagcgc cgggatctgc gcgctggtcg tactcgccct cgcgcgctcc ggtagtgcgc
     2821 aggtcgagcc ttcgggcgtc tggcgcaccc tccacactcc gcacttccgg attcacttcc
     2881 gtccaagcta ccgggacgtc gcgctccgcg aggcccgcga ggccgagcgc gcctacggac
     2941 tgctggccac cgagctgcac ccacccaggg gcagcgtgga tatcaccctc tcggacgatt
     3001 tcgacggtgc gaatggtctg accaccgtct ttccaaccga tcgcgtgacg ttgtttctgg
     3061 ctcctcccgc cacggatccc gcgctcgccc agtacgacga ctggttgcga ctcgtttccc
     3121 agcacgagct gacgcatgtc tttcacctcg atcgcgtccg aggcgtgtgg ggggtgatgc
     3181 agactgtctt cggccgcgcg ccgggacttt ttcccgagga gtatcagccg agctgggtga
     3241 ccgaaggcct cgcggtctac tacgagtcga agttcaccac cggcgggcgg gtgcggggca
     3301 gttaccacag ccaggtgctc gccgccgacg ccgcggattc ttcatcgcgc cccagctcgg
     3361 atgcgctgct ctttacccgc tgggccgatg gctacgtgcc ctatgcctac ggatcgcggt
     3421 tctttcgttc catcgccgac gcactcggcg actccgtcat tccgcgcttc gtcgagcgct
     3481 ccgctgggca gtggatcccg tatcgagtgg ggcacccgct ctccggcgcg tcccccggcg
     3541 tgcagctcga ctcggcgtgg gaggcggaca cgaggcccgc ggcgggtccc gggactcccg
     3601 gcaccgtgct cgagcgcggg ctatacagtg agcccgtccc gcgcgtgtct cccgatcgcc
     3661 ggtgggtggt gtggctgcgt gacgatggac gcggaccccg ccggctcatc atggcggacg
     3721 cgcgcacgtg gaaggaatca cgcagtcact acgtgaacgc cggcgtcagc tacgactggg
     3781 tcggcgacac ggtgatggtg gcgcaggtcg atctgacggg accgcaccgg atccgcagtg
     3841 acctctaccg ttgggatccc gacggctcgt ggaccagact cacgagaggc gcgcgcatca
     3901 ccgaaccgcg tggcggcgga gggacgctcg tgagcgtgca ggtttcaccg gacgggaatc
     3961 acccgaccat ccccgaagtc cgcgacaccg ccggcaccga ctggggcgat gtcgtcccgt
     4021 ccgtcgaccg ccgatgggtc gcggccacgc ggcatcgcga tgggcactgg cagctggtgc
     4081 gctggccctg ggcgtctccc gactccgtgt cagttctgag cgaagggtcg tctctcgtgt
     4141 cgggcccgac ctggtggggc gatacgctgc tcttcgtgtc cgatgtgtcg gggttcccgc
     4201 aggtctatcg gtggaacgat tccaccggcg gcccggctgc ccgggcggtt accgcggagc
     4261 cgctcggggc ccgttcgccg gccatactgc cggacggacg gatcgcctac acgtcgctcc
     4321 gctccaccgg ttggaccttg atgagcgcgc ccatccgcaa tggcgcggcg ctgggccccg
     4381 taccgccggc ggcgccgttc gacagtgcgc ccgccgttcc cgtgcgcgaa acggggtaca
     4441 cggcgtggcc atcgctcgcg ccgcacttct ggattccggt gtacttcgat gcgggaccgt
     4501 cggggcgctt cggcggtgcg gtgacggccg ggtccgacgc gctcgggcgc tggtcgtacg
     4561 ccgcgatcgg catggcgtcc ctctctcccg tgcgtttccg cggggagctt tcggtcgtcg
     4621 ccgacctgct gccgcagccc gtcttcgatg cggacgtttc gaacgcctgg tccaacgtcg
     4681 gcgagacatc cggaggaacc gtcgtctcga agctggcgca ggacgccgac cttggggcca
     4741 ccttccggta cctgcgctgg cggacggcgg tccagctccg ggtggcggcg gagttcgagc
     4801 gtgcggagta cgtggcgact ccggacactg cgctcgcggc ggtgtgtatc ggatgcgcca
     4861 gccgggatca gatcggcgga tcggtcacgc tggccgtcgc ccacaattcg tccggcatct
     4921 tgtcggtctc cccgcagaac ggattcaatt ggtcgctgac gtaccggcgg cgaaaccagc
     4981 agggatcgcc cctcttcgcc gacgaatacc ggagcctcct cgcgctgtac gcggcactgc
     5041 cgggtcccgg ctttgcacca ccggtgctgg ccctccggtt cggcgtggga gagaccaacg
     5101 gcccactcct gcggacactc ggggtgggtg gtgtgtcgtc aggaacgtac gatctgggct
     5161 tcggacagac ggtgggtggc gtgcgggact tcccggtccg ggggtacgcc ggcggcgccc
     5221 tggtcgaccg ccgcgccgcg accggcaccg ccgagctccg gatccccctg gcgctggtgg
     5281 gacggtccct cggccatctg ccggtcggcg cggacaaggt gtcgctccgt cccttcgccg
     5341 atgcggggga tgcgtgggcc cccggctcgt ctccgcgact cacgcgactc tggtcggttg
     5401 gtgcggagct cgccctcgac ctcacggtga attacgacgt gccgctcgct ctgcggctgg
     5461 gactcgcgga gccgatgacc gccccgcccg ccggtggtcc gcggcgcgca caagtctacg
     5521 tcgccttttc ctccgatttc tgaatcgctg cgccactcgg cgcgccggag gaattactgg
     5581 tccgattccg gcgaccactt gacgaacccc cgaacgtccg gtgtctccgg ggtataatga
     5641 aggacgcatg agactcctgg ccttgctcgt cgtcacggcc accaccgtcg ccgccgccca
     5701 gtcccccgac cagcgcgcgg cgctcgaacg tctccgtgac tcactcgaaa ccagctcgga
     5761 ctccgtcgcg ctgaaaggcc tcgaggctgc caccatcgag gtcgcgaaag tcaatcgcga
     5821 cgaccctctc atccatctcc ggctcggatt catcgcgtat cgcctcggcg agctcgcgaa
     5881 ggagagccac cactacgacg acgccgcggg ggaattcgag tgggcatcgc agctcaaacc
     5941 cgactggccc tatccgtggt tcggcctggg ccgctcggag ctggcgatcg gtgcgcactc
     6001 gtccatcgcg gccgagaacc tcaaacagtt tctgcacctc gatcatctct ccaaggcgat
     6061 caacgcgtat tcgaaggccg ccgcggtcga ccccacgttc gccgagccgg tgatcgagct
     6121 ggtcgaaacg gccctgcggc agcgggtgcg cgcggaaacg gacgtcgcgc tgcgcgtggc
     6181 ccgcacggcg gccacgtcgg cgagcggcat tcccgcgttc cagatggcgc gaggacggat
     6241 cgaacgggaa gtgggggaag gggattcggc gcgggccgcg ttcgaggcgt acctcaagga
     6301 cggcggtgat tccgggatcg ggctgctcga gctcgcgcgg accgactact acacgcatcg
     6361 cgacagcatc ggacacgcgg actacttcgc gggcgcgcgt gccgcggggt cggtggcggc
     6421 gatccggatg taccggacgg acttcagctg gatcgccact ccgcaggagc tcacggcctt
     6481 cgacagtctg cggaccagtg aagcgcgcgc gaactggctg aaagagttct gggccaagcg
     6541 tgacgtcgcc gacgtgcggg agccgggctc acggctggcc gagcactacc gccgctggtt
     6601 cgtcgcgatg cggtcgttcg gattggtgag cccccaccgc cattacgatt tcacggaggt
     6661 ctaccggagc ccgcaggatc tgatggatga ccggggcgtg atctacatgc ggctcggacc
     6721 cgccgacaag gtcggcgcct tctcgggctc gggcggcacc gccgcatcgg gggtggaacc
     6781 gaacgagagt tggctgtacc ggcggccggg tggggatctg atcttccatt ttgtgtcccg
     6841 gggcgacgta caggatttca agctggtgga gagcctcacc gacgcgctga gtttccagga
     6901 ccgccagcgg ctgcaactgt cgggcgacac gatgaacgtc gtgcgggggc tgttcgcgtc
     6961 gcgctcgacc ttcggtccga tgtacgaacg catcgggaac acgatcacga gcagcaactt
     7021 tctcccggcc atcgcggagg accggcagcg ggggacgtac tcgatcggcg taggcaccac
     7081 caccgacgac aaccacctcc ggttcgaccg gcccctcgat cccgccgtcg agggatacct
     7141 ggtcggtgcc tccgactcgg gtggggagca ggagctccac ctggtgttcg ccatcccggc
     7201 cgaggagctc acgccggtgg gggcggcgtc cggtgtgctg taccccctgc gcttccggct
     7261 ctacgtctcg accctgagtg acagcctggt cgggcgcatc gacacgctcc gcgtgttcgg
     7321 cgcgccccag attctccgga gcccgtcgta cctgacgggg cagctggagg tgcccgttcc
     7381 cgccggcgac taccgctatc gcctgctcat cgagtcgaag gacggggtga cgggcgcggt
     7441 ggcctcggac acgatcgcgg cggcttcgtt ggatggccgc gggttcgcga tgtccgacct
     7501 ggtggtgggg aagacgggcg gcctctggtg ggagcagcac ggggacaccg tgtacgtgaa
     7561 tccgctcgac cggttcccgg ccgggggcga cgcgcagctc tactacgagg tctacgggct
     7621 gcctgtgggc ggcgcatatc acacggagat caaggtcgaa cggaagggtg gcgggtcgat
     7681 cttcggcgcc atccgccgcc tgttcggcgg gaatcacccg cccgtgcagc tcgagttcga
     7741 cgcggctgcg accggcccgg tgacccgcgt gcaccggacg gtggcactgc gtgacgtttc
     7801 gaacggaacg tacgatctga ccctccgcat caccgatccc gcgaccggtc acgtgctgac
     7861 gcgggagcga cacttcgagg tggtttccgc caactagatt gacactccct cgcgccgcgc
     7921 cctaagtttc ccgcactcct tgcggagatg ggatgtactg ttcccgctgc ggcactcaga
     7981 acgaagacaa cgcgaagttc tgcaagtcct gcggcctgga cctgaccgct accactccgg
     8041 tccatgccat cagcaccggt cagcaggaag tcaccgaaat cgatctggtc cgtgaccagc
     8101 tgaaagagga gtacgagatc ctcgacgagc tgggacgcgg tggcatggcc atcgtcttca
     8161 aggcccgcga gaagcagctc gagcgggacg tcgcgatcaa ggtcctgccg ttctccctcg
     8221 cgttcgacaa ggaattcgtc gagcgcttcc agcgcgaagc ccgcacctcc gccaagctcg
     8281 agcaccccgg gatcattccg atctaccggg tcgggaagtc gggccgggtg atctacttcg
     8341 tcatgaagtt cctgcgcggg aagccgctct cggcggtgct ctcgcagcgg ggcgccctgc
     8401 cgcccgccga catccgccgc atcctcacgg aggtgggccg ggcgctgggg tacgcgcaca
     8461 agagcaacat cgtccaccgc gacatcaagc ccgacaacat catgttcgac gacctcggcc
     8521 aggccgtggt cacggacttc gggatcgcga aggcggcgag
//
LOCUS       DASUBP010000044         7834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_96302, whole genome shotgun sequence.
ACCESSION   DASUBP010000044 DASUBP010000000
VERSION     DASUBP010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7834
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96302"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VFU75_02010"
     CDS             <1..276
                     /locus_tag="VFU75_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HEU5465630.1"
                     /translation="SNAKNRLSALIKRVQKGESVTITDRGVPVAKLVPVQLGAGVPAR
                     IVALAQQGLVRLPERVPDTDWLDLPRPTPTPGPSVVDYLLEDRREGR"
     gene            273..698
                     /locus_tag="VFU75_02015"
     CDS             273..698
                     /locus_tag="VFU75_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HEU5465631.1"
                     /translation="MRFWDASALVPLLVNEPRTGEMVSVQREDPSAAVWWATPVECWS
                     ALARAQREGRLTDTDVVDAARLLSEAGERWTEVPPLDRVREQAARLVRLHPLRAADAL
                     QLAAALVLSDFEPRTLPFVTLDDRLASAARREGFEVLGV"
     gene            695..3337
                     /locus_tag="VFU75_02020"
     CDS             695..3337
                     /locus_tag="VFU75_02020"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU5465632.1"
                     /translation="MTTPLQRLETSLADRYHIERELGAGGMATVYLAHDLKHERRVAL
                     KVLKPELAAVLGADRFVVEIKTTASLQHPHILPLFDSGTADGFLYYVMPYIEGETLRD
                     KLNRETQLGIDEAVRITKEVADALDYAHRHGVIHRDIKPENILLHDGRPMVADFGIAL
                     ALSAAAGGRMTETGLSLGTPHYMSPEQATAEKDLTNRSDIYSLGCVLYEMLTGNPPHV
                     GASAQQIIMKIVTEDAPLVTSVRKSVPPNVSSAVAKAVERLPADRFETASDFAHALAD
                     PQFRWDAGRPGERAPGGRLVSVRRDWRLWAAAAGLAVVAAGVGRWTAGSRGAERVVAR
                     FAIPVAAQGGITSQSVNALALSSNGRTLVFASRGASGAEAQLYTRGLDELDAHPIPGT
                     QGGTSPIFSPDGKRLAYLGPGGVYVMTLPFSGGSASLVPNSQAQALSVILWLDNEHLL
                     ASDNSGGIVSIGLDGKTRIIAEPDPQAHEQSLFLEDVLPDHQTILAVPAGGYNIVGPV
                     VAIDSRTGHRTRVLDLTVNAAWYADGCLLWGLPSGTLQGALFDAARLKVTGAPVTVAE
                     HVHQNIGGSGQAAVSASGSLVYAAEEPLSLVMLDRAGHRTVLAEGKRFHSPRYAPDGT
                     RVALDLMENGARDVWTFDLRQHTLSRLSFESDGHDPVWSADGRGITYQHNAGVWRRRS
                     DGTGTPDSAYTGPDAAQALEYERDGSVLAASYGRSGAINVAWARTEGAKFTPLISSLF
                     TEEAATLSRDGKWLAYASDETGREEVYVRRFPGGPKVVTSSLGGREPRWAPDGRTLYY
                     IGQVDGNPTLMAATMELGEAAVVQHRAALFDASDFDPASPHANWDISPDGTHFVIAQQ
                     APLNSVIYILNVTEEIRRRSGRHE"
     gene            3330..5972
                     /locus_tag="VFU75_02025"
     CDS             3330..5972
                     /locus_tag="VFU75_02025"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU5465633.1"
                     /translation="MSSLDRLTRALADRYRIERELGAGGMATVYLAQDLKHHRRVAIK
                     VLHADLSAVLGPERFHKEIELTAGLQHPHILPLFDSGGADGLLYYVMPHVEGETLRTR
                     LTRETQLPVADAVRIASEVADALEYAHRHGVIHRDIKPENILLRDGHALVSDFGIALA
                     VVEAGGERMTQTGTSLGTPQYMAPEQAMGERHIDGRADVYALGVVTYEMLVGEPPFTG
                     PTAQAIIARVMTEEPRGLILQRRSVPEAVEIAVLTAIQKLPADRFRTPAEFRSALAGG
                     DGTTVPAARVSRPPRFARRDLWWGGLAAAVAIGGFLAGSRGGGVRAPLVFGLARKVTW
                     EPGLEIEPALSPDGRYVAYAQGGSEIMRIHVRQVSGGRPIPLSEDDSGTQTNPQWSPD
                     GSRILFLSRGGVFSAPSSGGSARPELPAPPAGPVTSAAWGPDGTTIAYTVGDSLYLRP
                     ASGTTRGLATVTDPTLCQWAATGRALACVSGNSWYSRVGHFLGNLSPSRIVVIQVRNG
                     QVTTVTDSTSINQSPVWSRDGRWLYFVSNVLGPEDVYAQAVTADGRAGGAPVRLSVGL
                     GAHGISISADGTRIAYDVLQATANIWSLPFPPGKATQADARPLTSGAQVVEGISVSPD
                     GEWVYFDSDVSGHSELYRQRSPNPVLPFFSAVDAEQLSFDSLDDFSPAVSPDGREVAF
                     HSWRSGSRDVYLLPLGGGPEQKVVASPRQDSRAEWSPDGRAICYLIFGTGGLWMVRRD
                     AAGHWGEPIERLPFGSWQAWSPDGRWLAFAEQQAGGSLLVAAPDSGAPRVLVDTKRTG
                     IIAEEPHWSADGRTIYFKSHDPLGRAEFWAEPLSGGPPRLLIRFDDLSRPSLRPEWGL
                     GGGRMYFTVNDRQSDIWVMEATPK"
     gene            5981..>7834
                     /locus_tag="VFU75_02030"
     CDS             5981..>7834
                     /locus_tag="VFU75_02030"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU5465634.1"
                     /translation="MRRAALMVSLAVLSSVSAAAQIPAGLDSMLHKLYASRDFAGARF
                     GPSRWIEGGTAYTTVEPSAGGKGDDIVRYETATGARSILVSAKQMTPAGANDPLDFDD
                     YTWSADASKLLLFTNTKKVWRANTRGDYWVLDRKSGNLSQLGGGGEPSTLMYAKFSPT
                     GDRVAYVRHGDVYVESLADHKITQLTTGADSLHVSGMTDWVYEEEFGLRDGFRWSPDG
                     AKIAFWHFDMTGVRTFYLINDTDSLYPYITPIQYPKAGTTNSAVTAGVVSASGGNITW
                     LQLPGDPRQDYLPRMDWVGPNDIILQRMNRAQQVNRVMMANATTGAVTPVLTEQDSAW
                     VDLDNDIVWINGGKDFLWTSERDGWRHVYRVSRTGTTTKLVTNGPYDITGIEGVDEKG
                     GWLYFGASPDNATQRYMYRTKLDGSGKAERVTPASQSGSNFYNISPDAKWALHIHSST
                     SEVPTTELIKLPSHQTVRVLVDNARLAAAVKPYMHKVEFFKAPLPDGATLDGWMIKPA
                     NFDSTKAYPVLLYVYGEPAGQTVQDGWQGNQGLWYGALADQGYIIASVDNRGTPAPRG
                     RAWRKAVYGHFGVLSSQEQADAVRVMGQQHKYVDMTRIGVWGWSGGGSSTLN"
BASE COUNT         1263 a         2633 c         2646 g         1292 t
ORIGIN      
        1 tccaacgcca agaaccggct gagtgcactg atcaagcggg tgcagaaggg tgaatcggtg
       61 accatcaccg atcggggtgt gccggtcgcg aagctggtcc cggtgcagct cggcgcgggg
      121 gtgcccgcga ggatcgtcgc cctggcgcag caagggttgg tgcgcctgcc ggaacgcgtc
      181 cccgacacgg attggctgga tttgccccgc ccgactccga ctcccggtcc ctctgtggtg
      241 gactacctgc tcgaggaccg gcgggaagga cgatgagatt ctgggacgct tccgctctcg
      301 tcccgctcct ggtgaacgag ccgaggacgg gcgagatggt gtccgtccag cgcgaggatc
      361 cgtctgcggc cgtctggtgg gctacccccg tggagtgctg gtcggcgctg gcgcgcgccc
      421 agcgcgaggg acggctcacc gacacggacg tcgtcgacgc ggccagactg ctgtccgagg
      481 ccggcgaacg ttggaccgag gtgccgccac tcgaccgagt cagagagcag gctgcccgtc
      541 tggtacgctt gcatccgctc cgcgcggccg atgcccttca acttgccgcc gcgctcgtcc
      601 tctcggattt tgagccccgg accctgcctt tcgtgaccct cgacgaccgc ctcgcttccg
      661 ctgcccgccg cgaaggcttc gaggtactcg gcgtgtgacc actccgctcc aacgtcttga
      721 gacgagcctg gccgaccgct accacatcga gcgcgagctc ggggccggcg ggatggccac
      781 cgtctatctc gcgcacgacc tgaagcacga gcgccgagtg gcgctcaagg tcctcaagcc
      841 cgagctcgcc gccgtcctcg gcgccgaccg gttcgtggtc gagatcaaga ccacggccag
      901 tctccagcac ccgcatatcc tcccgctctt cgactccggt acggccgacg gcttcctcta
      961 ttacgtaatg ccgtacatcg aaggggagac ccttcgcgac aagctgaacc gcgagaccca
     1021 gctgggcatc gacgaggcgg tgcggatcac gaaggaagtc gccgatgcgc tggactacgc
     1081 acaccggcac ggcgtgatcc accgggacat caaacccgag aacatcctcc tccacgacgg
     1141 ccggccgatg gtcgcggatt tcggcatcgc gctcgcgctg tccgcggccg cgggcgggcg
     1201 gatgacggag acggggctgt cgctcggaac gccccactac atgagccccg agcaggcgac
     1261 cgcggagaag gatctcacga accggtccga tatctattcg ctcgggtgcg tgctctacga
     1321 gatgttgacg ggcaatccgc cgcacgtcgg tgcctcggcg cagcagatca tcatgaagat
     1381 cgtgaccgag gacgcgcccc tcgtgacctc cgtccggaag tcggtaccgc cgaacgtttc
     1441 gagcgcggtg gcgaaagcgg tcgagcgtct tcccgccgac cggttcgaga cggcgtcgga
     1501 cttcgcccat gcactggcag atccacagtt ccggtgggac gccgggagac ccggtgaacg
     1561 ggctccgggg ggacggctcg tcagcgtgag gcgggattgg cgcctttggg cggcggcggc
     1621 cgggcttgcc gtggtcgcgg ccggggtagg gcgctggacg gccggatcca ggggcgccga
     1681 gcgcgtggtc gcacgcttcg ccatccccgt ggcggcacag ggcggtatca cgagccaatc
     1741 ggtgaatgcg ctcgcgttgt cctccaacgg gcggaccttg gtctttgcgt cccggggtgc
     1801 gtcaggtgcc gaggcgcagc tctatacccg gggtctcgac gagctcgatg cgcatccaat
     1861 ccccgggacg cagggtggga ccagtccaat cttttcaccg gacggcaagc ggctcgcgta
     1921 tctgggaccg gggggcgttt acgtcatgac cctcccattc agtgggggat cggcgagcct
     1981 ggtgcccaac agccaggcgc aggcactcag cgtcattctc tggctcgaca acgagcatct
     2041 cctcgcatcc gacaacagcg gggggatcgt gagcatcggt ctggacggaa agacccggat
     2101 catcgccgag ccggatcccc aagcccatga gcagagcctg ttcctggaag acgtgttgcc
     2161 cgatcaccag accattctcg ccgtccccgc cggcggctac aacatcgtgg ggcctgtcgt
     2221 cgccatcgac tcccggacgg gacatcggac gcgggtgctg gacttgacgg tcaacgccgc
     2281 ctggtatgcc gatggctgcc tcctgtgggg cctgccgagc gggacgctcc agggagcgct
     2341 cttcgacgca gcccggctca aagtcacggg cgctccggtg acggtcgccg agcacgtcca
     2401 ccagaacatc gggggcagtg ggcaggccgc ggtctcagcc tcgggttccc tggtatatgc
     2461 ggccgaagag cccttgtcgc tcgtgatgct cgaccgggcc gggcatcgca ccgtgctcgc
     2521 cgaggggaaa cggtttcaca gcccccggta tgctcccgac ggaacccgag ttgcgttgga
     2581 tctgatggag aatggcgcgc gggacgtgtg gacgttcgat ctgcgtcagc acacgctttc
     2641 acgactctcg ttcgagagcg acggccatga cccggtttgg tcggcggacg ggcggggaat
     2701 cacgtatcag cacaatgccg gagtctggcg gcgacggagt gacggcaccg gcactcccga
     2761 ttcggcctac accggccctg acgcggcgca ggcgctcgaa tacgagcggg atgggtcggt
     2821 gctcgccgcc tcgtacggcc ggagcggcgc catcaacgtc gcctgggctc gtaccgaggg
     2881 cgcgaagttc actcccctca tctccagtct cttcaccgag gaggcggcca ccctctcccg
     2941 ggatgggaaa tggctggcat atgcctcaga cgaaacggga cgcgaagagg tgtacgtccg
     3001 caggttcccg ggaggcccca aggtcgtgac gtcgagcctc ggcgggcggg agccccgctg
     3061 ggcgcccgat ggccggacgc tttactacat cggtcaagtc gacggcaatc cgaccttgat
     3121 ggccgcgacg atggaactgg gtgaagcggc cgtcgtacag caccgcgccg cgctcttcga
     3181 cgcttcggac ttcgaccccg cctcacctca cgcgaactgg gacatttcgc cggacgggac
     3241 gcatttcgtg atcgcgcagc aggcgccatt gaattccgtg atctacattc tgaacgtgac
     3301 cgaggagatc cggcggcggt cgggtcgtca tgagtagcct cgatcgactc acccgggcac
     3361 tcgccgatcg ctaccgtatc gagcgggagc tcggtgccgg cggcatggcg accgtctatc
     3421 tcgcccagga tctcaagcac catcgccgcg tcgccatcaa ggtcctccac gccgacctct
     3481 ccgcggtgct cggccctgaa cggtttcaca aggagatcga gctcacggcc ggtctgcagc
     3541 atccccacat cctgcccctc ttcgactcgg gcggggccga tggactgctc tactacgtca
     3601 tgccccacgt ggaaggggag accctgcgga cccgcctcac gcgcgagacg cagctgcccg
     3661 tggcggacgc cgtgcggatc gcgtccgaag tggctgacgc gctcgagtac gcacaccgcc
     3721 acggcgtcat ccaccgggac atcaagcccg agaacatcct gctgcgcgac ggtcacgcgc
     3781 tggtcagtga cttcgggatc gctctcgccg tcgtcgaggc gggcggggag cggatgaccc
     3841 agaccggcac cagcctcggc acgcctcagt acatggcgcc ggaacaggcg atgggagagc
     3901 gccatatcga cggccgggcg gatgtctacg ccctcggcgt ggtcacctac gagatgctgg
     3961 tgggggagcc accattcact gggccaacgg cgcaagccat catcgcgcgg gtcatgacgg
     4021 aggagccgcg gggactcatc ctccagcgcc ggagcgtgcc ggaggcggtg gagatcgctg
     4081 tgctcaccgc cattcagaag cttcccgccg accgcttccg cactccggcc gagtttcgga
     4141 gcgccttggc gggaggggac gggaccacgg tccccgcggc ccgggtgtcc cgtccccccc
     4201 ggtttgcgcg ccgggacctg tggtgggggg gcctcgccgc cgcagtggcc atcggcggat
     4261 tcctggcggg atcgcgaggc ggaggggtgc gcgcgccgct ggtcttcggc ctggcacgca
     4321 aggtcacgtg ggagcccggt ctcgagatcg agccggcgct ttcgcccgac ggacgctacg
     4381 tggcctacgc ccagggcggc tccgagatca tgcgcatcca cgttcgacag gtttccggcg
     4441 gccgtccgat ccccctgagc gaagacgact ccggtactca gaccaatccg cagtggtcgc
     4501 cggacggatc gcgaatactc ttcctctccc gcggtggcgt cttcagcgcg ccgtcgtcgg
     4561 ggggatccgc cagacccgag ctgccggcgc cccccgcggg gcccgtcacc tcggcggcat
     4621 gggggcccga tggcacgacg atcgcctaca ccgtgggcga ctcgctttac ctccgccccg
     4681 cgtcgggcac gacccgtggt ctggcgacgg tcacggaccc gacactctgt cagtgggcgg
     4741 ccaccggccg ggccctcgcc tgcgtctccg gaaattcgtg gtactcccgg gtgggccact
     4801 ttctcgggaa cctgtccccc agccggatcg tcgtaatcca ggtccggaac ggacaggtga
     4861 cgacggtgac cgacagcacc tcgatcaatc agagtccggt ctggtcgcgg gacgggcgtt
     4921 ggctgtattt cgtttccaac gtgctcgggc ccgaggacgt ctacgcccag gcggtgacgg
     4981 ccgacggccg tgccggcggt gcaccggtgc ggctctcggt agggctcggg gcgcatggga
     5041 tctccatctc tgccgacggc acccggatcg cctacgacgt cctgcaggcc acggcgaata
     5101 tctggtccct gccgttcccg ccggggaagg ccacgcaggc tgacgcccgc cctctgacgt
     5161 ccggcgccca ggtggtcgag ggcatcagcg tgtccccgga cggtgaatgg gtgtacttcg
     5221 actcggacgt cagcggacac tctgagctgt atcgccaacg ctcgccaaac cccgtcctgc
     5281 cttttttctc ggctgtggat gccgagcagc tctctttcga ttccctggac gacttttccc
     5341 ccgccgtttc tccggacggc cgggaggtgg cgttccattc ctggcggtcg ggatcgcggg
     5401 acgtctacct gctgccgttg ggcgggggac cggaacagaa ggtggtggcg tctccccggc
     5461 aagactcacg tgccgagtgg tctcccgacg gccgggcgat ctgctacctc attttcggca
     5521 ccggcgggct gtggatggtg cggcgcgatg cggccgggca ttggggcgaa cccatcgagc
     5581 ggctcccgtt cggatcctgg caggcctggt ctcccgacgg ccggtggctc gcgttcgccg
     5641 agcagcaggc ggggggctca ctgctggtgg ctgctccgga ttccggtgcc ccgcgggtcc
     5701 tcgtggacac caagcgtacg ggaatcattg ccgaggagcc ccactggagc gccgatggaa
     5761 ggaccatcta tttcaagagt cacgatccgc tcgggcgcgc cgagttctgg gccgaaccgc
     5821 tcagcggggg cccaccccgg ttgttgatcc gcttcgacga tttgagccgg ccgtcccttc
     5881 gtccggagtg gggcctgggt ggcggccgca tgtatttcac ggtgaacgac cggcagagtg
     5941 acatctgggt gatggaagcg acaccaaaat aggaggagta atgcgtcgtg ctgcactgat
     6001 ggtctctctc gccgtccttt cttcggtttc ggccgcggca cagatcccgg ccggactcga
     6061 ttccatgctc cacaagcttt acgcgtcgcg ggacttcgcg ggcgcgcgct tcggcccctc
     6121 ccgctggatc gaagggggaa ccgcctatac caccgtcgaa ccctccgcgg gtgggaaggg
     6181 cgatgacatc gtccggtacg agaccgccac gggtgcccgc agcatcctcg tctcggccaa
     6241 gcagatgacc cccgcggggg cgaacgatcc gctcgacttc gacgactaca cctggtcggc
     6301 cgacgcgagc aagctcctgc tcttcacgaa tacgaaaaag gtctggcgcg ccaacacccg
     6361 cggggactac tgggtgctcg accggaagag cgggaatctc tcgcagctgg ggggcggggg
     6421 tgagccgtcg accttgatgt acgcgaagtt ctctcccacc ggcgaccgcg tggcctacgt
     6481 gcgccatggc gatgtctacg ttgaaagtct cgccgaccac aagatcacgc agctcacgac
     6541 gggcgccgac tccctgcacg tgagcggcat gaccgactgg gtgtacgaag aggagttcgg
     6601 gctccgcgac ggattccgct ggtctcccga cggcgcgaag atcgccttct ggcacttcga
     6661 catgaccggg gtccgtacct tctatctgat caacgacacc gactccctgt atccctacat
     6721 cacgcccatc cagtatccga aggccggcac caccaactcg gccgtcaccg cgggcgtcgt
     6781 cagcgcttcc ggcggcaaca tcacgtggct ccagctcccc ggcgatcccc ggcaggacta
     6841 cctcccgcgg atggattggg tcgggccgaa cgacatcatt ctgcagcgga tgaaccgggc
     6901 ccagcaggtc aaccgcgtga tgatggccaa tgccactacc ggcgccgtca ccccggtgct
     6961 caccgagcag gactcggcct gggtggatct ggacaacgac atcgtatgga tcaacggcgg
     7021 caaggacttc ctgtggacgt cggagcgcga cggctggcgc cacgtctacc gggtctcgcg
     7081 gactgggacg acgacgaaat tggtgaccaa cggcccctac gacatcacgg gcatcgaagg
     7141 ggtggacgag aagggcgggt ggctgtactt cggtgcgtcg ccggacaacg cgacgcagcg
     7201 ctacatgtac cgcaccaagc tcgacggttc gggaaaggcg gagcgggtga cgcccgcgtc
     7261 ccaatcgggc tccaacttct acaacatctc gccggatgcg aagtgggcgt tgcacattca
     7321 ctcgagcacg tcggaagtgc ctacgacgga gctcatcaaa ctgccgtcgc accagacggt
     7381 ccgggtcctg gtggacaacg cccggctcgc cgcggcggtg aagccctaca tgcataaggt
     7441 cgagttcttc aaggcgcccc tgcccgatgg cgccacgctg gacggctgga tgatcaagcc
     7501 ggcgaacttc gactcgacca aggcctatcc ggtgctgctc tacgtctacg gcgagcctgc
     7561 gggccagacc gtccaggacg gctggcaggg caatcagggt ctctggtacg gcgccttggc
     7621 cgaccagggc tacatcatcg ccagcgtgga caatcgcggg accccggcgc cgaggggacg
     7681 ggcgtggcgg aaggccgtct acgggcactt cggcgtgttg tcatcccagg aacaggccga
     7741 cgccgtgcgg gtgatgggcc agcagcacaa gtacgtcgac atgactcgca tcggtgtgtg
     7801 gggctggagt ggtggcggct cgagcacgtt gaac
//
LOCUS       DASUBP010000045         8032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_191848, whole genome shotgun sequence.
ACCESSION   DASUBP010000045 DASUBP010000000
VERSION     DASUBP010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 8032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8032
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_191848"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /gene="thrS"
                     /locus_tag="VFU75_02035"
     CDS             <1..922
                     /gene="thrS"
                     /locus_tag="VFU75_02035"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEU5465635.1"
                     /translation="HILVYAAQQRSYRDLPIRLAEIANVYRNERSGVLHGMLRVRGLS
                     MDDAHIFLREDQIEQEIFNLLDIVELVLGKTFGLNYRLDLATRPEKKLGADEMWDVAE
                     KGLEQALKRRGMAYRLDAGGGAFYGPKIDIKFNDAIGREWQGATIQLDFQLPERFKLE
                     YTGADNKPHRPVMMHRAIYGTLERFVGFLIEHFAGAFPLWLSPEQVRVLPIADGQLDA
                     ANRTGDALRAAGLRVWVDDRSETLNYKIRDAETHKVPYMAVVGQREADAGTVAIRARG
                     EGKKQVVLPLAEFVSKLQDEVRTRALTSLL"
     gene            949..2136
                     /locus_tag="VFU75_02040"
     CDS             949..2136
                     /locus_tag="VFU75_02040"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HEU5465636.1"
                     /translation="MNDQTTPVILSACRTPIGKYLGGLAALTAPKLGALVVKEAVKRA
                     KIDGAAVEEVILGNVLQGGVGQAPARQAAIHAGLPGVIPALTINKVCGSGLKAVMLAA
                     QAIKAGDEQCVVAGGMESMTNAPHYVYGMRGGIKAGDQNLVDGMIRDGLWDSFSNQHM
                     GNLAEYTAKKAGVSRADQDAFALASHQKAVAAMEACHFKDQIVPVEIPGKKGPTVVEK
                     DESPRKDTTLEALAALKPSFEKDGSVTPGNAPGLNDGASALVVTSLAFAKAHGLAPLA
                     RITGYATGGGEPKDLFFAPIVAVQNLMRKTNAKIGDYDLIEANEAFAVQALADGRALG
                     WDWDRVNVHGGAVALGHPIGASGARVLTTLLYALRDRKKTTGLATLCLGGGNAVALSV
                     EVM"
     gene            2133..2705
                     /locus_tag="VFU75_02045"
     CDS             2133..2705
                     /locus_tag="VFU75_02045"
                     /inference="COORDINATES: protein motif:HMM:NF014669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEU5465637.1"
                     /translation="MSEVSSVALVRSRSTVLRRSIAVTAGVVLVAISAHIAIPIPGTP
                     VPMTLQPLAVLLVGGALGAALGGSTLVLYLTLGIAGLPVFSPLGGPGGIASIIGTSGG
                     FLLAFPLAGATTGRIVGNGQSTVRVAAGALAGLVLIYLGGLAQLAILTGSVRAAATLG
                     TLPFIIGDICKLAIAVASLRLLTPSLRARL"
     gene            2746..3594
                     /locus_tag="VFU75_02050"
     CDS             2746..3594
                     /locus_tag="VFU75_02050"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HEU5465638.1"
                     /translation="MSVVAVVGAGTMGNGIAHVFAQAGWDTALLDVSQDALDRALATI
                     GGNLDRQVKKGTATPEQKALTLSRIKTDTALSAVAGAELVIEAATEHPQTKFEIFKTI
                     DQHASPSAILGSNTSSISITTIAAQTKRPAQVIGMHFMNPVPVMQLVEVIRGIETSDQ
                     TTARVVEVSKALGKIPVEVNDAPGFVSNRVLMPMINEAVFCVEQGVATPEAVDTVMKL
                     GMNHPLGPLALADLIGLDVCLAILQVLHRDLGEDKYRPSPLLAKMVAAGRLGRKSKRG
                     FYDYRG"
     gene            3578..4747
                     /locus_tag="VFU75_02055"
     CDS             3578..4747
                     /locus_tag="VFU75_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5465639.1"
                     /translation="MTTAVERPDVSEARAEIVALAREFAKTNIEPHAAEWDRTHHFAR
                     NVIDELGKLGFLGIATPESDDGLGLDTTTYLMALEEIAAADASISVSVSIHCGIPTTM
                     LLRHGTPAQRDRWLKPMARGELLAGFALSEPDAGSDAASLRAQAVRDGDGWVLNGTKA
                     WATNGGTADVMMVMVRTDRPDARKGAKGISTFIVPTNTPGYRAARAEDKMGLRASNTA
                     GIELENVRLPADALLGDEGMGFVYSMEGLVAGRLGIATQAVAIARRALELSLAYCAER
                     KQFDQPLETFEALQFKLADMATRVAAARALTHQAAAAKDAGQDVTVQASMAKLFASET
                     AMWVTTQAVQLFGGYGYMRDFPVEKLFRDAKVTEIYEGTSEIQRLIIARGLTRHD"
     gene            4740..5813
                     /locus_tag="VFU75_02060"
     CDS             4740..5813
                     /locus_tag="VFU75_02060"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEU5465640.1"
                     /translation="MTDSRSPGLFTSLGEHSHEQVSFFYEPSTGYRGIIAIHDTTLGP
                     ALGGTRFWNYTSDQAALTDALRLSRGMTYKAAVAGLNLGGGKSVIIGDNKTTKREPLF
                     RVHGRHVESLGGRYITAEDVGTSTADMEFIRTETTHVTGLLGKSGDPSPVTAYGTYRG
                     MKAAAKHRWGSDSLSGKTVALQGCGHVGYYLCKHLHEEGAKLIVTDIDAQRVKTVTQD
                     FGASAVGADEIFTQKADIFAPCALGAVINDETIPKLKVAIVAGAANNVLAEDRHGDEL
                     EKRGIVYAPDYVINGGGLINVNAELHDWSLERARAKAGEIYDTILRVFEIAGEEKIPS
                     YRAADRLAEQRIAAVAKVRQNFV"
     gene            5806..6249
                     /locus_tag="VFU75_02065"
     CDS             5806..6249
                     /locus_tag="VFU75_02065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEU5465641.1"
                     /translation="MSEKIWVGADHAGFELKEKLKAELKRLGYEPVDAGPAKLDPADD
                     FPDFGKPVAEAVSEGLVKRGILTCGTGLGMSYVANRFPQVRAAVVWTPEIAALSRKHN
                     DANILVLPSRFTNERQAMDILHTFLDTPFEGGRHERRVEKIERDD"
     gene            6239..7510
                     /gene="glyA"
                     /locus_tag="VFU75_02070"
     CDS             6239..7510
                     /gene="glyA"
                     /locus_tag="VFU75_02070"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEU5465642.1"
                     /translation="MTTDRRAPLREVDPQIHALIKREVERQEHGLELIASENFVSVAV
                     LEAMGTALTNKYAEGVPKKRYYGGCEVVDEVEQLAIDRAKQIFGAEHANVQPHSGAQA
                     NMAAYLAVLKAGETLMGLALPHGGHLTHGASVNFSGSMFRSVQYGVREDTGRIDYDQV
                     RDLARKEHPKLLIAGGSAYARVIEFPVLKAIADEVGATFLVDMSHFAGLVAGGVHPSP
                     VPHAQIVTTTTHKTLRGPRGGMILCRADLAKAIDKWVFPGTQGGPLEHVIAAKAVSFR
                     EALEPSFKDYAAQVVKNAQALAKAMVARGYAIVSGGTDTHLMLVDLRAKNLTGKEAEQ
                     LLDRAGITVNKNTIPGDPQSPFVTSGIRLGTPAVTTRGFKEAECGTVAELIDTVLTGK
                     DDATLAKVKGRVRELTDAFPLYAHRREPAHV"
     gene            7503..7910
                     /locus_tag="VFU75_02075"
     CDS             7503..7910
                     /locus_tag="VFU75_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR04138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Minf_1886 family protein"
                     /protein_id="HEU5465643.1"
                     /translation="MSEPDREPTTLDTAFARIREQDGAYHERAYLFVLAALEFAQGRL
                     PARRHLSGGELAWACRDFALEQFGLLAGEVLAFWGIRSTADFGRIVFLLIDVGLLARQ
                     PSDKVEDFEAVYEFADALRAGYAWPGLSGGSAP"
BASE COUNT         1382 a         2632 c         2777 g         1241 t
ORIGIN      
        1 gcacatcctc gtctacgcgg cccagcagcg cagttaccgc gacctgccca tccggctggc
       61 ggagatcgcg aacgtctacc gcaacgagcg gtcgggcgtg ctgcacggca tgctgcgggt
      121 ccgcggtctc tcgatggacg atgcccacat cttcctgcgc gaagatcaga tcgagcagga
      181 gatcttcaac ctgctcgaca tcgtggagct ggtgctcggc aagacattcg ggctcaacta
      241 ccggctcgat ctcgcgacgc gccccgagaa aaagctgggc gccgatgaga tgtgggatgt
      301 ggccgagaag ggcctcgagc aggcgctgaa gcgtcgcggc atggcgtacc ggctcgacgc
      361 gggcggcggg gcgttttacg gtccgaagat cgacatcaag ttcaacgacg cgatcggccg
      421 cgagtggcag ggcgccacca ttcagctcga tttccagctt cccgagcgct tcaaactcga
      481 gtacacgggc gcggacaaca aaccgcaccg gcccgtgatg atgcatcgcg ccatctacgg
      541 aacgctcgaa cgtttcgtgg ggttcttgat cgagcatttc gccggcgcct tcccgctctg
      601 gctgtcaccg gagcaggtgc gggtcctgcc gattgcggac ggtcagctcg acgcggccaa
      661 caggacggga gatgcgttgc gcgcggccgg gctgcgggtg tgggtggacg accgcagcga
      721 aaccctgaac tacaagattc gggatgcgga gacgcacaag gtcccgtaca tggccgtcgt
      781 gggacagcgc gaggcggatg ccggcacggt ggcgatccgg gcgcgcggtg aaggcaagaa
      841 gcaggtggtg ctgccattgg ccgaattcgt cagcaagctg caagacgagg ttcgcacccg
      901 ggcgctcacc tcattacttt gagggcacgg actggagact attaatagat gaatgaccag
      961 accacacccg tcatcctgag cgcctgccgc actcccatcg ggaagtatct gggggggctc
     1021 gccgcattga ccgcccccaa gctgggggcc ctcgtcgtaa aggaagcggt caagcgcgcc
     1081 aagatcgacg gggcggcggt ggaagaagtc attctgggca acgtgctgca ggggggcgtc
     1141 ggacaggcgc ccgcgcgcca ggccgcgatc cacgccgggc tccccggcgt catccccgcg
     1201 ctcaccatca acaaggtgtg cggctccggc ctcaaggccg tgatgctggc ggcgcaggcc
     1261 atcaaggccg gcgatgagca gtgcgtcgtc gccggtggca tggagtccat gaccaacgcg
     1321 ccccattacg tctacgggat gcgcggcggg atcaaggcgg gcgatcagaa cctggtcgac
     1381 ggcatgatcc gcgacgggct ctgggactcc ttctcgaacc agcacatggg caacctcgcc
     1441 gagtacaccg ccaagaaggc cggcgtgtcc cgggccgatc aggatgcctt cgcgctcgcc
     1501 agccatcaga aggcggtcgc cgcgatggag gcgtgccact tcaaggatca gatcgtcccg
     1561 gtcgagattc ccggcaagaa gggccccacg gtcgtcgaga aggatgaatc tccgcgcaag
     1621 gacacgacgc tcgaggcgct ggccgcgctc aagccgtcgt tcgaaaagga cggcagcgtc
     1681 acaccgggga acgcgcccgg actgaacgac ggagcgtcgg ccctggtcgt gacgtccctg
     1741 gcgttcgcca aggcgcatgg gctggcgccc ctcgcgcgta ttaccggata tgccaccggt
     1801 ggcggcgagc ccaaggatct cttcttcgcg ccgatcgttg ccgtgcagaa cctcatgcgc
     1861 aagaccaatg cgaagatcgg cgactacgac ctgatcgagg ccaatgaagc cttcgcggtg
     1921 caggccctgg ccgacggccg cgcgctcggt tgggattggg accgcgtgaa cgtgcacggt
     1981 ggtgcggtcg cgctcggtca ccccatcggc gcctcgggtg cccgcgtgct gaccacgctg
     2041 ctctacgccc tccgcgaccg gaagaagacc accggcctcg cgaccctttg cctcggcggg
     2101 ggaaacgccg tcgccctcag tgtggaggtc atgtgagcga agtctcgtcc gtcgcgctgg
     2161 tccgctcccg gtccacagtg cttcgccgca gcatcgccgt gacggcgggc gtggtgctgg
     2221 tcgccatctc ggcgcacatc gccatcccga taccgggcac gccggttccg atgaccctgc
     2281 agcccctcgc ggtattgctc gtgggtggcg cgctgggtgc ggcgctgggc ggatcgaccc
     2341 tggtgctcta tctcaccctg ggcatcgccg gactgccggt gttctcgccc ctcggcgggc
     2401 cgggcggcat cgcgagcatc atcggaacgt cgggcgggtt cctgctggcc ttcccgctgg
     2461 cgggcgcgac cacgggccgg atcgtgggga acgggcagag cacggtgcgg gtcgccgccg
     2521 gcgccctcgc ggggctcgtc ctgatctacc tgggtggctt ggcgcagctc gcgattctca
     2581 ccggatcggt tcgggccgcc gcgacgctcg ggacgttgcc gttcatcatc ggggatatct
     2641 gcaagctggc gatcgccgtc gcgtccctcc ggcttctgac gccttctctg cgcgcgcgcc
     2701 tctagggcgc gcgagtcgtt ttttgccagg agagggaatc ggacgatgag cgtggtcgcg
     2761 gtggtcggag cggggaccat gggcaacggc attgctcacg tcttcgcgca ggccggctgg
     2821 gacacggccc tcctcgacgt gtcccaggac gcgctcgacc gggcgctggc caccatcggc
     2881 ggcaatctcg accgccaggt caagaagggg accgcgacac ccgagcagaa ggcgctgacg
     2941 ctctcccgca tcaagacgga caccgcgctc tccgcggtcg ccggcgccga gttggtgatc
     3001 gaagcggcga cggagcatcc gcagaccaag ttcgagatct tcaagacgat cgaccagcac
     3061 gcctcgcctt cggcgattct cggttccaat acgtcgtcca tttccatcac caccatcgcc
     3121 gcgcagacca agaggcccgc gcaggtgatc ggcatgcact tcatgaatcc ggtgccggtg
     3181 atgcagctgg tcgaggtgat ccgcggcatc gagacgtccg accagacgac cgcgcgggtc
     3241 gtcgaagtgt ccaaggccct cggcaagatc cccgtcgaag tgaacgacgc accggggttc
     3301 gtgtcgaacc gcgtgctgat gccgatgatc aacgaggccg tgttctgcgt ggagcagggt
     3361 gtggcgacgc ccgaggccgt ggacacggtg atgaagctgg ggatgaatca tccgttgggg
     3421 ccgctggcgc tcgccgattt gatcgggctc gacgtgtgcc tcgccattct acaagtgttg
     3481 catcgggatc tgggtgagga caaataccgg ccgtcgccct tgctcgcgaa gatggtggcc
     3541 gcggggcgcc tgggccggaa gagcaagcgc gggttctatg actaccgcgg ttgagcgtcc
     3601 ggacgtgtcc gaggcccggg cggagatcgt cgccctcgcg cgcgagttcg ccaagacgaa
     3661 catcgagccc cacgccgccg agtgggaccg gacgcatcac ttcgcccgga acgtgatcga
     3721 cgagctgggc aagctggggt tcctcggcat tgccacgccg gagtcggatg acggactcgg
     3781 cctcgatacg acgacctact tgatggcgct ggaggagatc gccgccgccg acgcgagcat
     3841 ctcggtgtcg gtctcgatcc actgcggcat cccgaccacg atgctgctcc gtcacggcac
     3901 accggcccag cgcgaccgct ggctgaagcc gatggcacgc ggcgaactgc tcgcggggtt
     3961 cgcgctctcg gagcccgatg cgggatccga cgcggcctct ctgcgcgccc aggcagtcag
     4021 ggacggcgac gggtgggtgc tgaacggtac caaggcgtgg gccacgaacg gcggcaccgc
     4081 cgacgtcatg atggtcatgg tgcggacgga tcggcccgac gcgcggaagg gcgcgaaggg
     4141 catttcgacg ttcatcgtgc cgaccaacac tccgggctac cgggcggcac gtgccgagga
     4201 caagatggga ctgcgggcgt ccaacaccgc cggcatcgag ctcgagaacg tgcggctgcc
     4261 ggccgacgcc ctgctcggtg acgaaggcat gggcttcgtc tattccatgg aaggactcgt
     4321 ggcgggccgg ctcggcatcg ccacgcaggc ggtcgccatc gcccgccggg cgctcgagct
     4381 gtcgctcgcg tactgcgcgg agcggaagca gttcgaccag ccgctggaga ccttcgaggc
     4441 gctgcagttc aagctcgcgg acatggcgac ccgggtcgcc gcggcgcggg cactgacgca
     4501 tcaggccgcc gccgcgaagg atgccggcca ggacgtgacg gtgcaggcga gcatggccaa
     4561 gctgttcgca tcggagaccg ccatgtgggt caccacccag gcggtgcagc tgttcggggg
     4621 ttacggctac atgcgcgatt tccccgtcga gaaactcttt cgggacgcga aggtcacgga
     4681 gatctatgaa ggtactagcg aaatccaacg tctcatcatc gcccgggggc tcacccgcca
     4741 tgactgattc ccgttcgccc ggcctcttca cgtcgctcgg cgagcacagc cacgagcagg
     4801 tcagcttctt ctacgagccg tccaccggat accgcggcat catcgcgatc cacgacacca
     4861 cgctcggtcc ggcgctcggc ggcacgcgct tctggaacta cacgtccgac caggcggcgt
     4921 tgaccgacgc actgcggctc tcccgcggca tgacctacaa ggcggcggtg gccgggttga
     4981 acctgggcgg cgggaagagc gtcatcatcg gggacaacaa gacgaccaag cgggaaccgc
     5041 tcttccgggt ccacggccgc catgtcgagt ccctcggcgg ccgctacatc acggccgagg
     5101 atgtcgggac gtcgacggcg gacatggagt tcatccgcac cgagaccacg cacgtgaccg
     5161 gactgctcgg caagtcggga gacccgtcgc cggtcacggc gtacggcacg taccgcggga
     5221 tgaaggccgc ggcgaagcat cgctggggct ccgactcgct gagcggcaag acggtcgccc
     5281 tgcagggctg cgggcacgtc ggctactacc tgtgcaagca cctgcacgaa gagggcgcga
     5341 agctcatcgt caccgacatc gacgcgcagc gggtgaagac ggtgacgcag gacttcgggg
     5401 cgagcgcggt gggcgccgac gagatcttca cccagaaggc cgacattttc gctccctgcg
     5461 cgctgggtgc ggtgatcaac gacgaaacca tcccgaagct caaggtcgcg atcgtggccg
     5521 gtgccgcgaa caacgtcctc gcagaagacc ggcatgggga tgagctggag aagaggggca
     5581 tcgtctacgc ccccgactac gtgatcaatg gcggcggcct gatcaacgtc aacgccgaac
     5641 tgcacgattg gagcctggag cgggcgcgcg ccaaggccgg tgagatctac gacaccattc
     5701 tgcgcgtgtt cgagatcgcc ggcgaggaga agatcccctc gtaccgcgcc gccgaccggc
     5761 tggccgagca gcggatcgcg gcggtcgcca aggtgaggca gaactttgtc tgagaagatc
     5821 tgggtcggag ccgaccacgc ggggttcgag ctgaaggaga agctcaaggc cgagctcaag
     5881 cgtctgggct acgagcccgt tgacgccggt cccgccaagc tcgatccggc cgacgacttt
     5941 ccggacttcg gcaagccggt ggccgaggcg gtcagcgagg gcctcgtcaa gcggggcatc
     6001 ctgacctgcg gtacgggatt gggcatgtcc tacgtcgcga accggtttcc gcaggtccgc
     6061 gccgcggtgg tctggacccc ggaaatcgcg gcgctttcgc gaaagcacaa cgacgccaac
     6121 attctggtgt tgccgtcacg gttcacgaac gagcggcagg ccatggatat cctgcacacc
     6181 ttcctcgaca cacccttcga ggggggacgg cacgaacgcc gggtggagaa gatcgagcgt
     6241 gacgactgac cgtcgggctc cgctgcgcga agtcgatccc cagatccacg ccctcatcaa
     6301 acgggaagtg gagcggcagg agcacggcct cgagttgatc gcgagcgaga atttcgtgtc
     6361 ggtcgcggtg ctcgaagcga tgggcacggc gctcacgaac aagtacgccg aaggggtgcc
     6421 gaaaaagcgg tactacggcg gctgcgaagt ggtggacgag gtggagcagc tcgccatcga
     6481 ccgcgccaag cagatcttcg gggcggagca cgcgaacgtg cagccccact cgggcgcgca
     6541 ggcgaacatg gccgcgtacc tcgccgttct gaaggcgggg gagaccctga tggggctggc
     6601 cctgccgcat ggcggacacc tgacccacgg ggcgtccgtc aacttctcgg ggtcgatgtt
     6661 ccggtcggtg caatacggcg tccgtgagga caccgggcgc atcgactacg accaggtccg
     6721 cgacctcgcc cggaaggaac accccaagct gctcatcgcg ggcgggagcg cgtacgcccg
     6781 cgtcatcgag ttcccggtgc tcaaggcgat tgcggatgag gtgggggcga cgttcctcgt
     6841 cgacatgtcg catttcgcgg gcctggtcgc cggtggcgtg catccgtcgc cggtgccgca
     6901 cgcgcaaatc gtcaccacca cgacccacaa gacgcttcgc ggtcctcgcg gcggcatgat
     6961 cctgtgccgg gccgatctgg cgaaggcgat cgacaagtgg gtattcccgg gcacgcaggg
     7021 cggacccctc gaacatgtca tcgccgcgaa ggccgtgtcc ttccgcgagg cgctggagcc
     7081 gtccttcaag gactacgccg cccaggtcgt gaagaatgcc caggccctcg cgaaggcgat
     7141 ggtggcccgc ggatacgcga tcgtctccgg cggtaccgat acgcacctga tgctcgtgga
     7201 tctccgcgcc aagaacctca ccggcaagga agccgagcag ctgctcgatc gcgccgggat
     7261 cacggtgaac aagaacacga tccccggcga tccccagtcc cccttcgtca cgtcgggcat
     7321 ccgcctcggc acacccgcgg tgaccacgcg cggcttcaag gaagcggagt gcggcaccgt
     7381 ggccgagctg atcgacacgg tgctgacggg gaaggacgac gcgacgctgg ccaaggtgaa
     7441 ggggcgcgtg cgcgagctga ctgacgcgtt cccgctgtat gcccaccggc gcgaacccgc
     7501 ccatgtctga gccggaccgc gagcccacca cgctcgacac cgccttcgcg cgcatccgcg
     7561 agcaggacgg cgcgtaccac gagcgggcgt acctcttcgt gctcgcggcg ctcgagttcg
     7621 ctcagggccg ccttccggcg cggcggcatc tctccggcgg tgagctcgcc tgggcctgcc
     7681 gggatttcgc tctggagcag ttcgggctcc tggcgggaga ggtgctcgcg ttctggggca
     7741 tccgctccac cgccgatttc gggcgcatag tctttctctt gatcgacgtc gggctcctgg
     7801 cccggcagcc atccgacaag gtcgaagatt ttgaagctgt ctacgaattc gcggacgccc
     7861 tccgggccgg gtacgcctgg ccggggttga gcgggggaag cgcgccgtaa ggatccaccg
     7921 gaagagggag agggacgcgt gagcgacgtg cgcgcgccgg cagtcagcgg ccgcttctac
     7981 ccaggcaccc cttccgagct ggagcgcgcc atgcgggacc tgatggaccc cc
//
LOCUS       DASUBP010000046         4430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_287252, whole genome shotgun sequence.
ACCESSION   DASUBP010000046 DASUBP010000000
VERSION     DASUBP010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_287252"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            161..1411
                     /locus_tag="VFU75_02080"
     CDS             161..1411
                     /locus_tag="VFU75_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HEU5465644.1"
                     /translation="MSITRRGFVRAGGMALFSVGLDPLFLDRAAFALSSRLAVQPSVL
                     VCLFQRGAVDGLNMIVPHGDRIYYEERPRIGVPATDVVDLDGYFGLHPSLAALKPWWD
                     NKSLAAIHAIGSPDATRSHFDAQDYMESGTPGVKITPDGWLNRYCQHDREHQSSAFRS
                     VAFGPELPRILSGTAPALAIGDLKTFGLRVPQNAQGDRVTRAFEALYQGSSTGLLASS
                     SEEGFEAARQLKAADPTQYQPEHGAAYPNGRLGHSLLEIAQLIKADIGLQIAFADATG
                     WDTHVNQGASEGQLSARLKELGDGLAAFATDLGDRMHNIVVLTMSEFGRTIHENGNSG
                     TDHGHATAMFALGGPVNGGRVLGKWPGLAPEQRYEGRDVAVTTDFRDLFAEILARHLG
                     SADTASVFPGYSVDPAKFVGAIKG"
     gene            1540..3465
                     /locus_tag="VFU75_02085"
     CDS             1540..3465
                     /locus_tag="VFU75_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5465645.1"
                     /translation="MSDPIATLLAAAERADGVPAFWKSTAQALEAELKAPVRLTYRGL
                     NDSGEITAGAFTTGTASVAITDADGRRVEAFAGRPLTEADVTGVHNILINANHLAVMV
                     GKRIALERERRLGTFIVELSRWLFAAPETELLLRYTMQGVTSLIDAQGGYVALREAPG
                     HTAPDAVTVVTAIGMAHDFEGRVLNLERSTTGRVVRTGEAVITSDASAEPDASPPDWT
                     NADRVRSALIAPLHTSSGVVGAIGVIRFRGADGERDPHPFTITDLHYFTAVAAQIAGG
                     LELSQAVAAARAAANRAGAMVNASPLPLALVDPLGRVQQLNEAGRAVFGLSSDSAAIG
                     KHLDGLGLSTSELPLHSILAGRKDGEPWNGRVVVTQPGGDRRLCDCTITNLTGLGSPH
                     LLVALYDRTDELRTQRELIAREKLATVGEIAGGVAHEVNNPLAAIRMEAELLGRGSSD
                     PEVGAAASTIVREVDRAARIVRSLLRLARRADSTPVRVNINELIRDVAEIRQRVLRAE
                     NIEVMTVLEPDSPVVLGLGQELQQVVINLVTNAEYAARGGSRSPAMVRVSSQERGGWV
                     RLTVDDSGPGIPPSIRHRIFDPFFTTKGPDEGSGLGLSICQRVVAEVGGKIWLEDSDL
                     GGARFVVELPAAPESPG"
     gene            complement(3475..3855)
                     /locus_tag="VFU75_02090"
     CDS             complement(3475..3855)
                     /locus_tag="VFU75_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465646.1"
                     /translation="MSFDRHSVPLPLAMHLASYTVVGLFCGFAGGLNLIDPGHILSGA
                     LPLLIWSGFSIGYVFGVLMGRSEVIPLGFLSSLGYVVVGYFAWTRGFWKFGADWRLGA
                     LCLALGLIGLFALGMYRKRIPVTS"
     gene            3894..4394
                     /locus_tag="VFU75_02095"
     CDS             3894..4394
                     /locus_tag="VFU75_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5465647.1"
                     /translation="MLVTRTYLELATPQAFRPAFGSFPDVRLERVPDPPPQLYRECYR
                     TVGEAYHWRDRWHWTDAEISAHLAQPEITLFVAHRGKALAGWYELRRVPDEGSVEVAY
                     FGIVPAEFGHGFGKHLLSCAVRDAWAMAPGRVWLHTCTLDHPNAVPNYKARGFVPYRT
                     ETYEVD"
BASE COUNT          762 a         1501 c         1463 g          704 t
ORIGIN      
        1 aattctcgat cagatcaagg acgtcgcgga ctcaggagcc gcccggaatt ttgcggtggg
       61 gttggcgttg gggggaccgg attttcaacg gcagtgacga gacggccaaa tgactagacg
      121 gctagacggt tagacggcta gttctgaatt ggagactccg atgagcatca ccagacgtgg
      181 atttgtcagg gccggtggaa tggcgttgtt cagcgttggt ctcgatccgc ttttcctgga
      241 ccgcgcggct tttgctctct ctagccgtct agccgtccag ccgtctgttc tggtgtgtct
      301 cttccagcgc ggcgccgtgg acggcctgaa catgatcgtg ccacacggag accggatcta
      361 ctacgaagag cggccccgga tcggcgtgcc ggccacggat gtggtcgatc tggatggcta
      421 cttcgggctc cacccgtcgc tcgccgcgct caagccctgg tgggacaaca agagcctcgc
      481 cgcgattcac gcgatcggct cacccgatgc cacgcgctcc catttcgacg cccaggacta
      541 catggagtca ggcacgccgg gcgtgaagat cacaccggac ggctggctca accgctactg
      601 ccagcatgat cgcgagcacc agagctcggc attccgctcc gtagccttcg gccccgagct
      661 gccgcgcatc ctgtcgggca cggcgccggc cctcgccatc ggggacctca agactttcgg
      721 tctgcgggtc ccgcagaacg cccagggaga ccgggtgacc cgggccttcg aggcactcta
      781 ccaaggctcg tccacgggcc ttctcgcgtc gtcatcggaa gaggggttcg aggcggcccg
      841 ccagctgaag gccgccgacc ccacgcagta ccaaccggag catggcgccg cgtatccgaa
      901 cggcagactg ggacattctc tgctcgagat cgcccagctc atcaaggccg acatcggact
      961 ccagatcgcc ttcgccgacg cgaccggctg ggacacccac gtgaatcagg gcgcgagtga
     1021 aggccagctt tcggcgcgcc tcaaggagct cggcgacggc ctcgcggcat tcgccaccga
     1081 tctgggcgac cggatgcaca acatcgtggt gctcacgatg tcggagttcg gccgcacgat
     1141 tcacgagaac gggaacagcg gcaccgacca tggccacgcg accgcgatgt tcgcgctcgg
     1201 cgggccggta aatggtggaa gggtcttggg gaagtggccc ggcctcgcac ccgagcagcg
     1261 gtacgaaggc cgagacgtcg cggtgaccac cgacttccgc gatctcttcg ccgaaatact
     1321 cgcccgccat ctcgggagtg ccgacacggc ttcggtcttt cccggatact cggtggaccc
     1381 ggcgaagttc gtgggggcga tcaagggctg aaaaggcgtc aaggagaagc gtcgaggaga
     1441 accgtcgagg aactacgtac gtacgcctct gacgcatctc ctggacgtgg ttccttgacg
     1501 cagttccttg acgctttttc ttgacgtagt ttgttgtctg tgagcgaccc catcgccacc
     1561 ctcctggccg ccgctgaacg ggctgacggg gttccggcgt tctggaagag caccgcccag
     1621 gccctcgagg ctgagctcaa ggcgcctgta cgcctcacgt accggggcct gaacgactcg
     1681 ggcgagatca ccgccggagc gttcaccacc ggaacggcct ccgtcgcgat caccgacgcc
     1741 gacggccggc gggtcgaggc cttcgcggga cgtcccctca ccgaagccga cgtcaccggg
     1801 gtccacaaca tcctcatcaa cgccaaccat ctggcggtga tggtcggcaa gcggattgcc
     1861 ctcgaacgcg aacgccgact gggcacgttc atcgtcgagc tttcccgctg gctcttcgcg
     1921 gctcccgaaa ccgagctgct gctccggtat acgatgcagg gcgtgacctc cctgatcgac
     1981 gcccagggag ggtatgtcgc gcttcgcgaa gcgccggggc acacggcgcc cgacgcggtc
     2041 accgtcgtga ccgccatcgg catggcgcac gatttcgagg gccgcgtgct gaatctggag
     2101 cgcagcacca cgggccgtgt ggtgcgcacc ggcgaggcgg tgatcacgtc cgatgcgtcc
     2161 gccgagccgg acgcgagccc ccccgactgg accaatgcgg accgcgtgcg ctccgcgctg
     2221 atcgcgccgc tgcacacgtc cagcggcgtg gtcggtgcca tcggcgtcat ccgcttccgc
     2281 ggcgccgacg gagagcggga cccccaccca ttcaccatta ccgacctcca ctacttcacc
     2341 gccgtcgcgg cccagatcgc cggcggtctc gagctgtcgc aggccgtcgc cgccgcccgc
     2401 gccgcggcga accgcgccgg cgcgatggtc aatgccagcc ccctgcctct cgcactggtc
     2461 gaccccctgg gacgggtgca gcagctgaat gaggccggcc gggccgtctt cggactgtcc
     2521 tcggactcgg cggcgatcgg caaacatctg gacggcctcg ggctttcgac tagcgagttg
     2581 ccgctccatt cgatcctcgc cggccggaag gacggtgagc cgtggaatgg gcgggtggtg
     2641 gtcacgcagc ccggcggcga ccgccgcctc tgcgactgca ccatcaccaa cctcacggga
     2701 ctggggagcc cacatctgct ggtggccctg tacgaccgca ccgacgagct gcggacgcaa
     2761 cgcgagttga tcgcgcgcga gaagctggcg acggtcggtg aaatcgcggg cggcgtggcc
     2821 cacgaggtga acaaccccct tgccgccatt cgcatggaag cggagctcct ggggcgcggc
     2881 agctcggacc ccgaagtcgg cgccgccgcg tcgactatcg tccgcgaggt ggaccgggcg
     2941 gcacgtatcg tccgctccct cctgcggctg gcgcgccgcg ccgactccac gccggtgcgc
     3001 gtgaacatca acgagctgat cagggacgtc gcggagatca gacagcgcgt gctgcgggcg
     3061 gagaacatcg aggtcatgac ggtgctcgag cccgattccc ccgtggtgct cggcctgggt
     3121 caggagctcc agcaagtcgt catcaacctc gtcaccaacg cggaatacgc ggcgcgcggc
     3181 ggatcacgct ctccggcgat ggtgcgcgtg tcgtctcagg agcgcggcgg gtgggtgcgg
     3241 ctgacggtgg acgattcggg ccccggcatc ccaccgtcca tcaggcaccg gatcttcgat
     3301 ccgttcttca ccaccaaagg ccccgacgaa gggtcggggc tcggactctc gatctgtcag
     3361 cgggtggtgg ccgaggtcgg cgggaagatc tggctcgaag actctgatct ggggggcgcg
     3421 cgcttcgtgg tcgagctgcc cgcggccccc gaatccccag gctaaagcct gggctcagga
     3481 cgtaacggga atccgcttcc ggtacatccc caaggcgaag agaccgatca agccgagcgc
     3541 caggcagaga gcgccgagcc gccagtcggc cccaaacttc cagaacccgc gggtccacgc
     3601 gaagtaccct accacgacat aacccaggct cgacaggaag cccagcggga tgacctcgct
     3661 ccggcccatg aggacgccga acacgtaacc gatgctgaag ccggaccaga tgaggagggg
     3721 caatgcgcca ctcaggatgt ggccggggtc gatcaggttc agtccacccg cgaagccgca
     3781 aaagaggccg acgaccgtgt agctggccag gtgcatggcg aggggcagcg ggacactatg
     3841 gcgatcgaag ctcatggaca tgtggggttg gaggacggcg taagatacat acgatgctgg
     3901 tgacccgcac ctacctcgag ctcgccacac cgcaggcctt ccggcccgcg ttcgggagct
     3961 ttccggatgt ccgcctggaa cgggtgcccg acccgccgcc gcagctgtac cgcgagtgct
     4021 accgcaccgt gggtgaggcc taccattggc gggaccgctg gcactggacc gacgccgaga
     4081 tcagcgccca tctcgcgcaa cccgagatca ccctcttcgt cgcgcaccgc gggaaggcgt
     4141 tggcgggatg gtacgagctg cgccgcgtgc cggatgaggg ctccgtcgag gtcgcgtact
     4201 tcggcatcgt acctgccgag ttcggacacg gtttcggaaa gcacctgctc tcctgcgcgg
     4261 tgcgcgacgc ctgggcgatg gcgcccgggc gcgtttggct gcacacctgc acgctcgatc
     4321 accccaacgc ggtgccgaac tacaaggcgc gcggcttcgt gccttatcgc accgaaacgt
     4381 acgaggtaga ttgacccaat gaccctctcc cgcaaggcga agctcatcat
//
LOCUS       DASUBP010000047         3652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_859562, whole genome shotgun sequence.
ACCESSION   DASUBP010000047 DASUBP010000000
VERSION     DASUBP010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3652
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_859562"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="VFU75_02100"
     CDS             <1..215
                     /locus_tag="VFU75_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465648.1"
                     /translation="LPKIEVSRRVAGPPLWKPETAGTGSSGDWIAMVTDDANFRADFP
                     VFRFSDTSWLVTLGNLAWDRARILAP"
     gene            212..1450
                     /gene="glp"
                     /locus_tag="VFU75_02105"
     CDS             212..1450
                     /gene="glp"
                     /locus_tag="VFU75_02105"
                     /inference="COORDINATES: protein motif:HMM:NF045515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HEU5465649.1"
                     /translation="MTALAAEAAARLILEQVRPLNPVRRPLADCRGLVLAEEVLAPIA
                     VPPWDNSAMDGYAFRGADSRAGATLRVIEEVRAGGFPTRPVGPGEATRLFTGAPIPEG
                     ADTVIRQEDTTASAGTVQIVVPPAAGKNVRKRGEDLSPGATAVARGLELTPARLGVLA
                     SVAHAEPLVHPVPHVALLATGDEVVKLDRRSEILAGKKIASSNTYALAAAIVEAGGVP
                     VDLGIAPDTKDAVRERLRAAGDADLIVTTAGVSVGDHDLVRDALTSLGGTVTFSRVQI
                     RPGGPLNFGSLNGIPWIGLPGNPVSSLVTFELFARPAIRRLAGQRLPFRRAVPVVTAE
                     ALSLGPSLRHLLRVTLTPSSDGLPVAHLTGSQSSGVLSSMAKADALLIVPEDRHDVPA
                     GTILQAVVLDDPAHVAEPAF"
     gene            1447..2103
                     /locus_tag="VFU75_02110"
     CDS             1447..2103
                     /locus_tag="VFU75_02110"
                     /inference="COORDINATES: protein motif:HMM:NF014025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HEU5465650.1"
                     /translation="MNATIASAGHVIQDLLLVLTSVLFIVDPFATVPSFLAMTEKDTP
                     AFRRVLARRGAWTAAITLTAFGLGGALIFKLFGITIGAFKIAGGVLIGLNALDMVQAR
                     RSSQRETAVEKAEGIQKEDIGIMPLGVPMLAGPGAISTVMVLALGSKSLVTTIALYAS
                     IAITAYISYLTLAAASLVERRLGQTGMRILTRLMGLILCAIAIQFVLDGIRLSFPSAG
                     "
     gene            2262..3302
                     /locus_tag="VFU75_02115"
     CDS             2262..3302
                     /locus_tag="VFU75_02115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465651.1"
                     /translation="MRAATRGERLVLQATGDFEGLTIKNGELTPGAWGEGFADRRHPH
                     TYLHEFMLLAPDLLRDLAVPAHLSLGVGKGFVPFGTDDPMSRPTERFPVNHHLSQILE
                     RAMAMAGVTVGPAKLEGALFNGDEPQHPDQWPNLSRFGDSWSVRLTVEPVTGLEWEGS
                     RAFIRSPETRDVGGLDATKWSTALRWDRPLLGRPVYAMLEWARTREGDAFTLATVLFE
                     AQVTAGRHHPYFRLERTDRPEDERTLDPYRDVRPVIENSVLGVTRWTIGTLGYAVDAG
                     RTWKVSWKPFVEGSLIGIQSVGSGLFNPAVFYGTDRHWSLTTGIRLSWGMEDHRMGRY
                     DRSGMLMSMEHQ"
     gene            3299..>3652
                     /locus_tag="VFU75_02120"
     CDS             3299..>3652
                     /locus_tag="VFU75_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465652.1"
                     /translation="MIRRAPAALLFGLACHTADRIGPPAGGGSVTLAATVPIAPNYGV
                     HDTFIRNGIAIVCVWNSGVLIYDVGNGMKGGSPTNPVLVGSVVPSEDSVTAGPEIHNA
                     WWFWNPTNGQKKYLFL"
BASE COUNT          574 a         1260 c         1200 g          618 t
ORIGIN      
        1 gcctgcccaa gatcgaagtc tcgcggcgcg tcgccggccc gcccctgtgg aagcccgaga
       61 cggccgggac ggggagctcc ggcgactgga tcgcgatggt gacggacgac gcgaatttcc
      121 gcgcggattt cccggtcttc aggttcagtg acacctcctg gctcgtgacc ctcggcaacc
      181 ttgcctggga ccgggcgcgg attctcgcgc cgtgaccgcg ctggcggcgg aagccgcggc
      241 ccgtctcatc ctcgagcaag tccgtccgct caaccccgtg cgccggcctc tcgccgactg
      301 ccggggactc gtcctcgccg aggaggtgct cgcccccatc gccgtacccc cctgggacaa
      361 ctccgcgatg gatggctatg ccttccgcgg agcggactcc cgcgccggcg cgacgctccg
      421 tgtgatcgag gaagttcgcg cgggcgggtt ccccacaaga ccggtcgggc cgggtgaggc
      481 caccaggctg ttcaccgggg ccccgattcc cgagggagcc gacaccgtca tccgccagga
      541 agacacgacg gcgagtgccg gcacggtcca gatcgtggtc ccccctgccg ccgggaagaa
      601 cgtccggaag cgcggcgagg acctgtcccc gggagccacc gccgtcgccc gtggactcga
      661 gctcacaccg gcccggctcg gggtactcgc ctcagtggcg cacgcggaac cgctcgtgca
      721 tcccgtaccg cacgtcgccc tgctcgcgac cggggacgaa gtggtgaagc tcgaccgccg
      781 gtcggagatt ctcgcgggca agaagatcgc cagctcgaac acctacgctc tcgcagcggc
      841 catcgtcgaa gcgggcggcg tgccggtgga cctcgggatc gcccccgata cgaaggacgc
      901 ggtgcgggaa cggctgcgtg ccgccgggga tgcggatctg atcgtgacga cggcgggagt
      961 cagcgtgggt gaccacgacc tggtccgcga cgccctgaca tccctcggcg gaacggtcac
     1021 cttctcgcgg gtccagattc gccccggtgg tccgctcaac ttcggatcct tgaacggcat
     1081 tccgtggatc ggcctgccgg ggaatccggt ctcgtcgctc gtgaccttcg aattgttcgc
     1141 ccggccggcg atcagacggc tggcgggcca gcggctcccc ttccgccgag ccgttccggt
     1201 ggtgacggcc gaggcgctgt ccctgggacc ctcgttgcgg cacttattac gggtgaccct
     1261 gacaccgagc tccgacgggc tgccggtggc tcacctcacg ggctcccaaa gctccggcgt
     1321 cctctcttcc atggcgaagg cggacgcact cttgatcgtt ccggaggacc ggcacgacgt
     1381 accggcaggc accatcctgc aggcggtggt gctcgacgat ccggcgcatg tggcggagcc
     1441 ggcgttttga acgccacgat agcttccgcg gggcacgtga ttcaggacct tctcctcgtc
     1501 ctgacctccg tgctgttcat cgtcgatccg ttcgcgacgg tgcccagctt cctggcgatg
     1561 accgagaagg acacgccggc gttccggcgc gtcctcgccc ggcggggcgc ctggacggcc
     1621 gcgatcaccc tgaccgcctt cggtctcgga ggcgcgctga tcttcaagct gttcgggatc
     1681 actatcgggg cattcaagat cgcggggggc gtgctgatcg ggctcaacgc gctggacatg
     1741 gtgcaggcgc gccgcagctc acagcgggag accgcggtag agaaggccga gggcattcag
     1801 aaggaggaca tcggcatcat gcccctcggc gtaccgatgc tcgcgggacc gggcgccatc
     1861 tccaccgtga tggtgctcgc ccttggctcc aagagcctcg taacgacgat cgcattgtac
     1921 gcgtcgatcg cgatcaccgc atacatttcg tatctgacgc tcgcggccgc gagtctcgtg
     1981 gaacggcggc tgggccaaac gggtatgcgc attctgaccc gtctcatggg actcattctc
     2041 tgcgccatcg cgattcagtt cgtcctcgac ggcatcagac tctctttccc ttccgcgggc
     2101 tgatcaccgc ggttctcgcc acctttggcc ctggggtttc ccggggccaa acaccctcca
     2161 ttcaactcgg cgcgcaggcg atccccgtcg ccaccgaatc caacgctgtc ccccgcgggc
     2221 gctcgtacgg ggaagcccgg ctggtacagc cgatggtcat gatgcgggcc gcaacccgcg
     2281 gggaacgcct ggtgctgcag gccaccggag acttcgaagg tctcaccatc aagaacggag
     2341 agctgacgcc gggcgcctgg ggtgagggat tcgccgatcg ccggcacccg cacacctacc
     2401 tgcacgagtt catgctcctg gcacccgacc ttctgcgaga cctcgccgtc cccgctcacc
     2461 tgtccctcgg cgtcggcaag ggattcgtgc cattcggcac cgacgatccc atgtcccgcc
     2521 ccaccgagcg gtttccggtg aaccaccacc tgagtcagat cctcgaacgg gccatggcga
     2581 tggcaggcgt cacggttggt ccagccaagc ttgaaggtgc cctgttcaac ggtgacgagc
     2641 ctcagcaccc ggaccaatgg ccgaatctct cgcggttcgg tgactcctgg tcggtgcggc
     2701 tcacggtcga accggtgacg ggacttgagt gggaggggtc gcgggcgttc atacggtccc
     2761 ctgaaacccg tgatgtgggg ggcctggacg ccacaaagtg gagcaccgcg ctgcggtggg
     2821 acaggcccct gctcggacgg ccggtgtacg caatgctgga gtgggcccgg acgcgggaag
     2881 gcgacgcgtt cacgcttgcg actgtcttgt tcgaagccca ggtgaccgcc ggacggcacc
     2941 atccgtactt ccggttggag cgtaccgacc ggcccgaaga cgaacggacg ctcgacccct
     3001 atcgcgacgt gcgtcccgtc atcgaaaact cggtcctcgg cgtcacgcgc tggaccatcg
     3061 gaaccctggg atacgcggtg gacgccggac gcacctggaa ggtgtcgtgg aagcccttcg
     3121 tggaaggttc actgatcggc atccagtccg tgggcagtgg gctgttcaac cccgcggtct
     3181 tttacgggac tgaccgccat tggtcgctca ccaccggcat ccgcctcagc tggggaatgg
     3241 aggaccaccg catgggacga tatgatcgtt cgggaatgct tatgtccatg gagcaccagt
     3301 gatccgccgg gcgccagcgg cgctgctctt cggattggcc tgtcacaccg ctgaccgcat
     3361 cggccctcca gccggtgggg gaagcgtcac cctcgcggcg accgtcccga ttgccccgaa
     3421 ctacggcgtg cacgacacgt tcatccggaa cggcatcgcc atcgtctgcg tctggaactc
     3481 gggcgtcctg atctatgacg tcggcaacgg catgaagggc ggcagcccca ccaatcccgt
     3541 cctggtcggg agcgtggtcc ccagtgaaga cagcgtcact gcgggccccg agatccataa
     3601 cgcctggtgg ttctggaacc ccacgaacgg ccagaagaag tacttgttcc tg
//
LOCUS       DASUBP010000048        23499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_791624, whole genome shotgun sequence.
ACCESSION   DASUBP010000048 DASUBP010000000
VERSION     DASUBP010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 23499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23499
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791624"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..661
                     /locus_tag="VFU75_02125"
     CDS             110..661
                     /locus_tag="VFU75_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEU5465653.1"
                     /translation="MRCLALNASFEPLTMVPVRRALRLVIDGKAEIVEADHDAVIRSE
                     RLRLPRPAVIRLVKFVHVPRRFRRQVTNTFLFARDEYRCQFCGRTQRDLRDRECLTRD
                     HLVPLSRGGSNAWTNVVTACSSCNTRKGNLLPEEVGMHPMRAPVEPHFVHLSWAVRRL
                     TAIQTKYIRLFYGSDVLRTLERL"
     gene            658..1071
                     /locus_tag="VFU75_02130"
     CDS             658..1071
                     /locus_tag="VFU75_02130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_267462262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYCXC family (seleno)protein"
                     /protein_id="HEU5465654.1"
                     /translation="MSAKRLPWIIAGAAVLLLVGLVAFPRRGAASLHPDPRPGITADS
                     VVPPKMVMNTPGAAEAYAVARQIPQVLDGLYCHCDCSQHMGHRSLLTCFTGDHAAQCD
                     ICMGEAMMAGQMAAAGNSLDQIRKAIDAQFGTTRS"
     gene            complement(1068..1700)
                     /locus_tag="VFU75_02135"
     CDS             complement(1068..1700)
                     /locus_tag="VFU75_02135"
                     /inference="COORDINATES: protein motif:HMM:NF025692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HEU5465655.1"
                     /translation="MRSPSLLGGLLLVVLGAGVARAQADHPTLTVMLPDSVTPRVQAV
                     ALLSDPRFPDLLKSGFPLRLHFRLELWRARSSWFDQFVSDASWDAVVRHDPLADDFVL
                     YRSTGEIKRFPSPELLETALERPFSVKLVPRSDGRYYYVCRLEITTLSDSDLDELTRW
                     LKGEVGPAVSGEGSIGDALARGAQRALVRIAGLPRLTLEARSTVIERGNR"
     gene            complement(1700..3436)
                     /locus_tag="VFU75_02140"
     CDS             complement(1700..3436)
                     /locus_tag="VFU75_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEU5465656.1"
                     /translation="MKTLLLGVAFLCAPVLVSAQDTAIVINPETNGVHLTPPELPRQV
                     ADSAIAFFNSAATSRLVGRTEIPAGTVWHGDVAVRNGPVILAGRIEGSLLVINGDLVL
                     KPGAVVSGAVLVVGGELSGSDSSRVEGKARVFYEALGFRMKGDSLQYSPNLRRRYGQF
                     AAAYVFNTPETRSALTIATGGTYDRVEGLPLVFGPAFDWQMTPRDHLRLDALGVFRTA
                     GDLSGARSDLGYRLRGELRTGRYRPVSLAFSAFNVISPIEDWTLSGSEVGWASFLFRR
                     DYRDYFADQGWGLKLSVQPERQLTADLEWRKESRRSIASLDPFSIFRNEEVWRPNPPI
                     DGGHYRTLGLGISLDTRNDPDEPTTGWWARLKIERSTSPDVAPQTVPAFIRGPIPTDG
                     SYEFSRFFLEARTYQRIGGFGRANLRLLFGGWLGGDPLPLQDRLSLGGPDPMAGYPFR
                     YTGCNREIVDPAFGSSQPAACDRVLLFQGEYRGHINLRWAYDPGEDQPQSGLLYRLEG
                     PDLVVFGDAGQAWLVGNGPGRIPSGRIPTIGSWLADLGLGIDWGGVGLYAAKAVTIGQ
                     PVRLTLRLRHRF"
     gene            complement(3433..4815)
                     /locus_tag="VFU75_02145"
     CDS             complement(3433..4815)
                     /locus_tag="VFU75_02145"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5465657.1"
                     /translation="MSSRVLLIDDESNIRRMVGALLQGEGFETSEAPNGAAGLAALTE
                     TPPDVIMLDLMMPGELDGLATLERLKHRLPDIPVVMMSGKASLADAVRATKLGAFQFL
                     EKPLTPEGVLVAVRGALELARTLAENRRLREALGQSDPLVGSSAAMEELRSLIARIAP
                     TEARVLITGESGTGKELVASAIHRQSGRSTRPFITVNSAALPKDLVESEMFGHERGAF
                     TGATDRRLGRFELADGGTLFLDEVGDLGAEAQAKLLRVLETGVIERIGGEKPITVNVR
                     VIAATNKDLSKAVKDGHFRDDLLFRLNVIPVHIPPLRDRAGDIPALVSHFAQRQATRL
                     GRVVKLDAGAVQLLATYPWPGNVRELANSVERLAILAGGAGITAEDVSRVLPRDGMAT
                     PAPAGWEDVALTDALDRHEQSLITRALSAAKGNVADAARRLSTDRANLYRRMRRLGIE
                     PPRERDGTTP"
     gene            complement(4812..6017)
                     /locus_tag="VFU75_02150"
     CDS             complement(4812..6017)
                     /locus_tag="VFU75_02150"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5465658.1"
                     /translation="MPLPFRRRIFAILVAMTAIPTAMAVTGLVLSVRASGPMAGARAS
                     LEEVAASARTMLKTVDTTQLSSRERRAMDHHLDQISASLTLAQRAQTLRNYSSAGFAA
                     VIIVLGAVLLFVAVRVAGHLSRQLSRPIDELVGWTRLIRRREPLPGGPPQRGAPEFEA
                     LRQAVRELAAALETARERELESERLRAFREVARRVAHEIKNPLTSMRIAVDQLERSLA
                     VAEGAPNGRATTAVEVMGAETNRLERLAREFADFGRLPEGPKSDVDLVELLEELGRTG
                     VPDGVRVQVSGPATRRVLSGYYEPLRRAFSNLLRNAAEAMGGTGRINVLVVPAGGSGF
                     QVTIADHGPGIPADIKARLFEPYFTTKADGTGLGLALVRQTIEAHGGSVSVGDTPGGG
                     ATFTITFPS"
     gene            6088..6507
                     /locus_tag="VFU75_02155"
     CDS             6088..6507
                     /locus_tag="VFU75_02155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HEU5465659.1"
                     /translation="MRSHTHYLWFNTKQRQEIIDITDTVETQVAESGVREGMVLVSAM
                     HISASVFVNDHESGLWHDILKWLEGTIAPWDPPRYRHNETGEDNAAAHLRSLTVGHEV
                     IVPITAGKLDLGPWQRVFYGEWDGQRKKRVIIKVLGD"
     gene            6741..8327
                     /locus_tag="VFU75_02160"
     CDS             6741..8327
                     /locus_tag="VFU75_02160"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEU5465660.1"
                     /translation="MQQPHAHLSRPVHKEPPAHAPNAALLIDFDNVTMGIRSDLQTEL
                     RRLLQSEIIRGKVAVQRAYADWRRYPQYIVPLSESSIDLIFAPAYGSNKKNATDIRLA
                     IDALELVFTRPEIGTFILLSGDSDFSSLVLKLKEYGKYVIGVGIRESSSDLLIQNCDE
                     YYSYNELAGFQKESDIVHERWDVWKLVVEAVKQMTAHNDVMRSDRLKQVMQEIDPAFD
                     EKDAGFSRFTKFVQEAAHRGLIHVTRRENGQFEIALGGAASQAPAASPGAPAEPEAPA
                     ERRSSFVGDAFGLLKQALGQSRDEEPVDTPALEEAAPKAHDEGSSQPPALGARSYHEE
                     EDEPPMPQSSAPPPPYSTPVAGHIPARSARFRRGSKGGPRPINTPVAGSPRVGPVETP
                     VASGNSDSNSGSERRERPERGDRPRGGRGRGARGGSRSEHAEGSGERPERGERGERGG
                     RSRRGGRGRGRGGSGGTGEGNRERLAGESPRAEASPVAPSAPAPHAPPPAPKKAPEPD
                     TSGESFWTRVKRGLTGGGNV"
     gene            8324..8911
                     /locus_tag="VFU75_02165"
     CDS             8324..8911
                     /locus_tag="VFU75_02165"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEU5465661.1"
                     /translation="MTGRPFPDDFYARPTPLVARRLLGHLLVSTIGGRRTVGRIVETE
                     AYVGPDDPACHAWKGRRSPMVESLYGPPGRAYVYFTYGMHWCMNAVTRREGFPSAVLL
                     RALEPLEGLEAMRRRRGRGIPDGRLCAGPAMLCQALGVTGRQDGKSLAVGSLRLLRDP
                     HVPRFKVAVGPRIGITRAVDWPLRFWVADSRWTSR"
     gene            complement(8967..9659)
                     /locus_tag="VFU75_02170"
     CDS             complement(8967..9659)
                     /locus_tag="VFU75_02170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465662.1"
                     /translation="MKLVSRLTLCALAVALLVSPRLLNAQTTGGSHAFQWYWGGGGGA
                     FIYKTNLQPLHFDPIVGGHWLITGKRTALYLSYEQAFFTSSAVAQIQDANSGSGVRNV
                     SFSNVRRLSIGVMAIPIDGHIQPMIGGGFSIMQILNPAIDCSGADATTTCASFSDSSL
                     AAQAASDQASKAFAWGAFGLQINFGRLGLYGLASVTSSAQQFMLDGPTYSVVGGIRYS
                     LGSSKEDITEQN"
     gene            complement(9802..10995)
                     /locus_tag="VFU75_02175"
     CDS             complement(9802..10995)
                     /locus_tag="VFU75_02175"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEU5465663.1"
                     /translation="MSLASRLAAELQQFQKDGVYKRLNYLDSPQGARVKMEGRGDVMI
                     LSSNNYLGLCNVPEVVKAGRDALDRYGAGTGSVRFICGTFTVHRELEQALARFVGCES
                     SLSYVSCWNANEGLCPTLLTEQDIVISDQLNHASIIDSIRLAKAITKCQTAVYKHSDM
                     ADLEDRLKAASGARLRVIFTDGIFSMEGDIARLPEIVALAKKYDAVVAMDDSHATGVL
                     GKTGRGTAEHFGMLGQVDIITSTLGKALGGAAGGFTAGPASLTDYLTQRSRPQLFSNA
                     LPPTVAASALAAVQYTERHPELVTRLRENAQYFRGRLGELGFKPLPGETPIVPVILGE
                     TSKAIQMSEMLLAEGVFVTGFGYPVVPQGHARVRCQISAAHSRDDLDFALGAFKKVGG
                     QLGLI"
     gene            complement(10992..12023)
                     /gene="tdh"
                     /locus_tag="VFU75_02180"
     CDS             complement(10992..12023)
                     /gene="tdh"
                     /locus_tag="VFU75_02180"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEU5465664.1"
                     /translation="MQAIVKETRAPGLTVKAVPDPAPPAAGEVLIAVRHAGVCGTDLH
                     IADWDAWASGRMHPPTILGHEFAGEIVKVGPGVTELSAGQLVTAEGHIICGHCLQCRT
                     GNGHICKNTRIIGVDRDGAFANYIVMPASNVLTLDGIPTEIGAIMDPMGNAFHTVLTA
                     DIPGSTVFVVGCGPIGCFAVGIARAAGAAKVIASDVNPKRLALAAKMGAHVTIDAKKE
                     DVTKAVLAATGGEGVDVVCEMSGIPSALHQAFATVRMGGRVQLLGIPKDQVPIDFANE
                     IIFKGITVYGVVGRKMYETWHQMRRFLSTGLFDPRPVITHRFPLAKIDDALAAIRSGD
                     AGKVILEIS"
     gene            12091..12810
                     /locus_tag="VFU75_02185"
     CDS             12091..12810
                     /locus_tag="VFU75_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465665.1"
                     /translation="MAIAAAVTVGPFSATAQVPDTAVSFRGIVMRDTGSAWALFIPVP
                     AQVRNIRFNLLHLSKVSGIDRDENRYGEVSGHLVADDAGSPVLEVAKLKLMSIPGEVE
                     RSVSRSFTEHAEVAASILPNQFAFPDSGASTRVRLRPVIAFAITNHSDAPIDFSFHDN
                     KLVCASVLPSNRTQEPIWTGAWPVGPYLTSIQINLSSTVREAVTLPDSVRLPPGHYKL
                     RVWLCEAADYNAVTVFDVIKR"
     gene            complement(12823..12990)
                     /locus_tag="VFU75_02190"
     CDS             complement(12823..12990)
                     /locus_tag="VFU75_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465666.1"
                     /translation="MTSLPMYLEDEPIGIILRMGREALKPAKIWAYVWCADGEAGEDH
                     PHQHLPLDRQR"
     gene            complement(13101..13943)
                     /locus_tag="VFU75_02195"
     CDS             complement(13101..13943)
                     /locus_tag="VFU75_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465667.1"
                     /translation="MLNTVAQGSPHATGPTRRPPRSQKQEYQEFLLQRIEEYKNTVPR
                     EELLRIGDEAVRELESGEGQYLLTEVLLLEHVDRIISRRLKLPSFRRWSQNHRALRTA
                     QREPTHWGLESSDPVAQGVKRLEAGDLVLVVGAAALPHALFVAAHDAEVFLLDQELGA
                     VESAEGRAVSEQLAGRFQALVVNFGGWLPDLRPALTVIEPNALATADAGDRENLVRRL
                     QACTRSGGIHVILSVDPDGPGVLPLAPDAVQSLYGEWQVERRKRRGRGEGFVATKPVR
                     QSDT"
     gene            complement(13937..14791)
                     /locus_tag="VFU75_02200"
     CDS             complement(13937..14791)
                     /locus_tag="VFU75_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEU5465668.1"
                     /translation="MPNPTAVRRVLTGILLANLAVVLVKVGVGLASGSLGVLGSALDS
                     AIDSANNVLALIVTAVAAKAPDEEHPYGHGKFETLGALALVGFLCVSSFELLRGAVNH
                     LTSGGHPLPVTNTQVGVLAATLLVNAGVARYERRRATELQSEILLADAAHTSADVLIT
                     AGVIVALLLARRGWWWADPVIAILVVGAILSVAYRVLIRTVPVLVDERVHPPERIAEC
                     ARGVPGVCDAYDIRSRGSANGRYAELTISVDGGATVAQAHQLADQVEARLKQQLDFHG
                     VTVHIEPC"
     gene            14866..15138
                     /locus_tag="VFU75_02205"
     CDS             14866..15138
                     /locus_tag="VFU75_02205"
                     /inference="COORDINATES: protein motif:HMM:NF033789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autorepressor SdpR family transcription factor"
                     /protein_id="HEU5465669.1"
                     /translation="MRDHLFKALADPTRRKILELLAAKDRTAGELAEEFPIAFASVSH
                     HLGVLKNAGLVAVEREGQHMRYRLNTTVFQEIARYLVKTFSGGADA"
     gene            15131..15775
                     /locus_tag="VFU75_02210"
     CDS             15131..15775
                     /locus_tag="VFU75_02210"
                     /inference="COORDINATES: protein motif:HMM:NF025015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEU5465670.1"
                     /translation="MRSRWFGLVIAALALVATWLAWDKLPPHMATHWDASGHINGYSS
                     RAFGALLAPAMMIGLTLLFQVLPALDPRRANYAKFADTYWVIANAVILFLGIVHLMVL
                     AYGLGYGVSMTRVLPLGLGVLFIALGNVLPRVEPNWFVGIRTPWTLSSDTVWRKTHRA
                     GGFVFLLGGCALLIEGITPLGNVLPVMIGTIVVTALTPVVQSYLLWKGEQGDRA"
     gene            15762..16727
                     /locus_tag="VFU75_02215"
     CDS             15762..16727
                     /locus_tag="VFU75_02215"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5465671.1"
                     /translation="MIALSLALLQAPQQKADTVAPGAAVESPFVATSSGWTLYGTLTV
                     PKAAAGPVPVVLIVAGSGPTDRNANSPAGIRSNTYAQLAWRLAERGIASLRYDKRGIA
                     ASRGAMDSAVVAQTTMQDYANDVRNLADSLHTDHRFSRVILVGHSEGSALSIMAAHDG
                     APVAGVVSMAGMGRPFATVLREQLAAQLDSNTLRMYDTSMTAYLAGRDPPVPSYLASL
                     FVPALRNYLRSMMAFDAKEVGQLHCPVLVLQGDYDLQVTVADAEALHAADPKSTLVIF
                     AGDTHMFKIAASKDRMAEVPTYVDPTVPIDPKVVATISDWIGNIR"
     gene            complement(16693..17472)
                     /locus_tag="VFU75_02220"
     CDS             complement(16693..17472)
                     /locus_tag="VFU75_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5465672.1"
                     /translation="MSKLRQTGFEPLGTTGPDVRTWTRDYPARYLIPAHYHDRDQLVH
                     ASRGVMTVAAGATTWVVPPRRAVWVPAGVSHEITTSGVVEMRTLYLRSRTVPPMELSV
                     VSVTPLLEELILEACRVGELHRRISRHRHLIALIIDQLREIQMVPLQLPYPRDARAQK
                     VAHAILAYPEPGALEDLCQYAGASRRTIERLFVSEAGMGLGRWRQQARLMHGMRLLAE
                     GAKVSHAALEAGYATPSAFISMFRKALGVTPTECFRSSPKS"
     gene            complement(17621..18775)
                     /locus_tag="VFU75_02225"
     CDS             complement(17621..18775)
                     /locus_tag="VFU75_02225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF022137.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEU5465673.1"
                     /translation="MNVAITAKGAARWRAGHPWVYRSDVAEEPGDQAGVVRVTDRKGK
                     YLARALYSPKSEIRLRLLTTEDVEIDREWWRSRIAAAAAKRTGIDATAWRVVHGEGDG
                     LPSLVIDRYGPYAVAQLLSAGVESAREHIGAAIQDVLHPEGILLRNDAAVRRHEGLPL
                     EVSLLSGTVPESVEVTESGVRYLAAPWQGQKTGGFLDQRENRVMLAEHARPGGRAADF
                     FAYHGSFTIHLAKVAGSVLAVDSSAEALVRGAKNAALNGFGNVTWKEANVFDLLREFE
                     GRGEAFDTIALDPPAFAKTKASVDRALAGYKEINLRAMRCLAPGGVLFTCSCSYHVHR
                     RDFEAMLLEAAQDSGRRLEVLRFTGQSSDHPEILTIPETGYLKGALLRRA"
     gene            complement(18772..19143)
                     /gene="aroH"
                     /locus_tag="VFU75_02230"
     CDS             complement(18772..19143)
                     /gene="aroH"
                     /locus_tag="VFU75_02230"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004106 - chorismate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEU5465674.1"
                     /translation="MTTRILRGLRGATTATANTSDAILDATAELLAALQKANAFAPED
                     IESAIFTSTPDLTAEYPARAARNLGWTGAPLLGAQEVPVPGGVPLCIRVLLHFYTARK
                     PGALKHIYLRGAAALRPDRST"
     gene            19147..19764
                     /gene="thiE"
                     /locus_tag="VFU75_02235"
     CDS             19147..19764
                     /gene="thiE"
                     /locus_tag="VFU75_02235"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU5465675.1"
                     /translation="MLVDRVRLMVLTDASLLKGRNVVEVCRRAVAGGATSIQVRWKPA
                     PAREVADVARALVGALSVPVLVNDRLDVALAVGAAGVHLGQDDVPVAALAAHLPPGFI
                     VGISVGTPMEASDADLRADYWSIGPCYATSTKSDAGAPLGPEGFGRLARLAPAGLPVI
                     GIGGITKDNAAAIRRAGAAGVAVIASVLASDDPERASRELRLSVA"
     gene            complement(19694..20713)
                     /gene="mltG"
                     /locus_tag="VFU75_02240"
     CDS             complement(19694..20713)
                     /gene="mltG"
                     /locus_tag="VFU75_02240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEU5465676.1"
                     /translation="MKKRIAQVLTVLLAACSTTPARGPQIAVTVPHGATLDVAMDSLS
                     ARGVIAHPFWFRIYARFVGLRSNLKSGTYAFHEDETWRVITEALSTGRGALVHFTTPE
                     GLMATEVADLARSRLGIPRDSFLLAIADPDLKSELGLPSNAPTVEGYLYPTTYLVPAK
                     IGARDLVRAMVREFLGQWDSTWTATLDSQHLSRQAIVTVASIVEAEVRYDPDRPYVAA
                     VYLNRLRRGMKLEADPTVIYAYGHRLKRVFEKNLLIRSPYNTYLHAGLPPGPIAQPGH
                     ASLDATIHPAEVPYLYFVAQPDGKHIFSVTYAEHLAAIQKVKRLKAAARAKRAPGHPT
                     PAPTR"
     gene            complement(20710..21138)
                     /gene="ruvX"
                     /locus_tag="VFU75_02245"
     CDS             complement(20710..21138)
                     /gene="ruvX"
                     /locus_tag="VFU75_02245"
                     /inference="COORDINATES: protein motif:HMM:NF015607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEU5465677.1"
                     /translation="MGRLLGVDWGEKRIGLALSDESRTLAQPLDTLTRRPGKRFPMAA
                     LQAHLATHGVTGIVVGLPLDAAGAEGDAATAARQLADDIARRTGLPVALWDERFTTAR
                     VLGAVREMGGKTRGRKGDVDALAATLLLQHYLDALRGSAV"
     gene            complement(21147..22400)
                     /gene="rho"
                     /locus_tag="VFU75_02250"
     CDS             complement(21147..22400)
                     /gene="rho"
                     /locus_tag="VFU75_02250"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEU5465678.1"
                     /translation="MDIAELKRKSVAELHAMAESLNIQNYSGMRKQDLIFRIEQSLLD
                     TDTVLRGEGVLEILPEGYGFLRSPDWNYLYGPDDIYVSPSQIKRFDLRTGDTILGQVR
                     PPKEGERYLALLKVENVNGEEPEKAKHRVAFDNLRPRYPEERIRLERKDGDLSMRVMD
                     ILCPIGKGQRGLIVSPPKAGKTILMQKLANSIIENHPEVYLIVLLIDERPEEVTDMEE
                     NVKGAEVISSTFDEPADRHVQVADMVIEKAKRMVEHGKDVVILLDSITRLGRAHNVVV
                     PHSGKILSGGVDANALQKPKRFFGAARNIEGGGSLTILATALIDTGSRMDEVIFEEFK
                     GTGNMEVILDRRIADRRVYPAIDVQRTSTRKEELLLAKDELNKVYLLRNFLADMPPVE
                     AIEFLLERMKRTKTNTEFFATMAQG"
     gene            22559..>23499
                     /locus_tag="VFU75_02255"
     CDS             22559..>23499
                     /locus_tag="VFU75_02255"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU5465679.1"
                     /translation="MNLMSFVERAETTSLPDREVVDALVHPAHAAPSPQLRDALETWR
                     GPYYWSDESDGRHLILTLVRPRRGERWWLHAALFVLTFASVTLSGAVLEGRLPYYGVL
                     GLLTSLAHPSARFWSTWASGLPFSIPLLGILLAHELGHYVAARRHGVNASPPFFIPAP
                     LISIIGSLGAFIRIRTILSDRRQLLDIGVAGPFAGFVVALPVLMVGLSQSHTHPLLAP
                     GSNLLLVVGHDIVPIGSSLLTSGVRALVLGGSGGLVLSPLAFAGWIGMFVTMLNLLPL
                     AQLDGGHALYAAAPRLHGRLALLFWGALAVLGWFTWQG"
BASE COUNT         3842 a         7711 c         7910 g         4036 t
ORIGIN      
        1 accccgcccc ttacagggca ggcttgcccg gtcctatcct ctgatgtagc ataccgttgc
       61 cgccccagaa tggtgagtga ggcggcaatt ttttgacggg ggagcccctt tgcgctgctt
      121 ggcactcaac gcctcgttcg agcccctcac catggtgccg gtacgacggg cgttacgcct
      181 ggtgatcgac gggaaggctg agatcgtcga ggcggaccac gacgctgtga tccgctccga
      241 acggctgcgc ctcccccggc cggcggtcat ccgcctggtc aagttcgttc acgtgccgcg
      301 ccggttccgc cgtcaggtca cgaacacctt cctcttcgcc cgcgacgagt atcggtgcca
      361 gttctgcggc cgtacccagc gcgacctccg tgatcgcgag tgcctcaccc gcgaccatct
      421 ggtcccgctc tcgcggggcg ggtcgaacgc gtggacgaac gtggttaccg cctgctcgtc
      481 gtgcaatacg cggaagggca atctcctgcc tgaagaggtg gggatgcatc ccatgcgggc
      541 gccggtggag ccgcacttcg tccacctgtc gtgggccgtg cgccgcctga ccgccattca
      601 gaccaagtac attcggctct tctacgggtc ggacgtgctg cggaccctcg agcgcctgtg
      661 agcgccaaac ggctgccttg gatcatagcc ggggcggccg ttctgctcct ggtggggctg
      721 gtcgcgttcc cccgtcgcgg cgccgcatcg cttcaccctg acccccggcc cggtatcacc
      781 gcggattcgg tcgtccctcc caagatggtg atgaatacgc cgggtgccgc cgaagcctac
      841 gcggtcgcgc gccagattcc ccaggtcctg gacggcctct attgtcactg cgattgctcc
      901 cagcacatgg ggcaccgctc gctcctgact tgcttcacgg gagatcacgc ggcgcaatgc
      961 gatatctgca tgggtgaggc gatgatggcg gggcaaatgg cggcggcggg gaattccctc
     1021 gatcagatcc gaaaagccat cgacgcgcag ttcggaacga ctcgctccta gcggttcccg
     1081 cgttcgatca ccgtcgagcg cgcttccaac gtcaaacgcg gcaagccggc gattcggacc
     1141 agggcgcgct gcgcccctcg cgcgagcgca tcgccgatgc tcccttctcc cgacaccgcc
     1201 gggcccacct ctcccttgag ccaccgggtc agctcgtcca gatcgctgtc ggagagcgtg
     1261 gtgatctcga ggcgacagac gtagtagtac cgcccgtcgg aacgagggac gagtttcacg
     1321 ctgaagggcc gttcgagcgc cgtctcgagc agctcggggc tggggaaacg tttgatctcg
     1381 cccgtggacc ggtacagcac gaaatcgtcc gcgagtggat cgtgccgcac caccgcatcc
     1441 cacgacgcat cgctcacgaa ttggtcgaac cagctggagc gggctcgcca gagctcgagc
     1501 cggaaatgga ggcgcagggg gaaacccgac ttgaggagat ccgggaaccg cgggtccgac
     1561 agcagggcga ccgcctgtac ccggggcgtg acggagtccg gcagcatgac tgtaagcgtg
     1621 ggatgatcgg cctgggctcg agcgacgccc gctcccagca cgaccagcag caacccgccc
     1681 agcagggagg gactgcgcac tagaagcggt ggcggagccg gagggtgagc cgcaccggct
     1741 ggccgatcgt caccgccttc gcggcgtaga gaccgacgcc accccagtcg atccccagcc
     1801 ccagatcggc cagccagctg ccgatggtgg ggatccggcc cgacggaatc cgtccgggtc
     1861 cgttgcccac cagccaggcc tgcccggcgt ctccgaacac gaccagatcg ggcccttcca
     1921 ggcgatacag gagcccggat tgaggctggt cttcgccggg atcgtaggcc caccggaggt
     1981 tgatatgacc gcgatactcg ccctggaaga gcaggacgcg gtcgcatgcg gccggttggc
     2041 tcgagccgaa tgccgggtcc acgatctccc ggttgcagcc cgtgtagcgg aagggatagc
     2101 cggccatcgg gtcgggcccg ccgagactca ggcggtcctg caggggcagg ggatcaccgc
     2161 ccagccatcc gccgaagagc agccggagat tcgcgcgtcc gaacccgccg atccgctggt
     2221 aggtccgcgc ctccaggaag aagcggctga attcgtacga tccgtcggtc gggatggggc
     2281 cgcggatgaa tgccgggacc gtttggggag cgacgtcggg cgatgtcgag cgctcgatct
     2341 tcagccgcgc ccaccatccg gtcgtcggtt cgtccggatc gttccgcgtg tcgagcgaga
     2401 tgcccaggcc caacgtgcgg tagtggccgc cgtcgatagg agggttcggc cgccagacct
     2461 cctcgttgcg gaatatcgag aaggggtcga gggacgcgat gctccgccgg gattcttttc
     2521 gccattcgag gtcggcggtg agctgccgct ccggctggac cgagagcttg aggccccatc
     2581 cctgatcggc gaaataatcc cggtagtccc gccggaagag aaatgacgcc catcccactt
     2641 cggatccgga gagggtccag tcctcgatgg gcgagatgac gttgaaggcg ctgaacgcga
     2701 ggctcacggg ccggtagcgg ccggtgcgga gttcaccccg gagccggtat ccgaggtcgc
     2761 tccgggcgcc cgacagatcg ccggccgtgc ggaagacgcc cagggcgtcg agccgcaggt
     2821 ggtcgcgcgg ggtcatctgc cagtcgaacg cggggccgaa cacgaggggc aggccttcga
     2881 cgcggtcgta ggtcccgccg gtcgcgatgg tcagcgccga ccgggtctcc ggcgtgttga
     2941 agacgtatgc ggcggcgaac tggccgtacc gccgtcggag gttgggtgag tactgaaggg
     3001 agtcgccctt catccggaat ccgagcgcct cgtaaaagac ccgggctttt ccttccactc
     3061 gcgagctatc cgacccggag agctcgccgc ccacgaccag cacggcgccg ctcacgaccg
     3121 cgccgggctt gagcaccagg tctccgttga tgacgaggag cgaaccctcg atccgccccg
     3181 cgaggatcac cgggccattc cgcaccgcga cgtccccatg ccacacggtt ccggctggga
     3241 tctcggtccg ccccaccaga cggctggtgg ccgcggagtt gaagaacgcg atcgccgagt
     3301 cggcgacctg gcgcgggagt tccggtggtg tgaggtgaac cccgttcgtc tcggggttga
     3361 tcacgatggc ggtgtcctgt gcgctcacca ggactggagc acaaaggaat gccactccca
     3421 gcaacagtgt cttcatgggg tcgtcccgtc tcgttcgcgc ggtggctcga tgccgagccg
     3481 ccgcatccgg cggtacagat tggcgcggtc cgtggacagc cggcgggccg catcggcgac
     3541 gttgcccttg gcggccgaca acgcgcgcgt aatcagggac tgctcatggc ggtcgagggc
     3601 gtcggtgagg gccacgtcct cccagccggc gggggccggt gtcgccatcc catcgcgggg
     3661 cagcacccgg gagacgtcct ccgcggtgat gcctgccccc cccgcgagga tcgccagacg
     3721 ctcgacggaa ttggcgagct cccggacgtt tccgggccag ggatacgtcg cgaggagctg
     3781 gaccgctcct gcatcgagct tcaccacgcg tcccagacgg gtggcctggc gctgggcaaa
     3841 gtgtgagacg agggcgggaa tgtcgcccgc ccgatcgcgg agtggcggga tgtgcacggg
     3901 aatcacgttc aggcggaaca gcaggtcatc ccggaagtgg ccgtctttga cggccttcga
     3961 gagatccttg ttggtggcgg cgatgacgcg gacgttgacg gtgatcggct tttctccccc
     4021 gatccgctcg atgactccgg tctcgagcac gcggagcagc ttggcctgcg cctcggcgcc
     4081 gaggtctccg acttcgtcca gaaagagcgt gcccccgtcc gccagctcga agcggcccag
     4141 gcggcggtcg gtggcgccgg tgaacgcgcc acgttcgtgg ccgaacatct ccgattccac
     4201 caggtctttc ggaagggccg cactgttgac ggtgatgaag ggccgggtgg agcgcccgct
     4261 ctggcggtga atggcggagg cgacgagttc cttgccggtc cccgactcgc ccgtgatcag
     4321 cactcgcgct tcggtcggcg cgatgcgcgc gatcaggctc cgcagctctt ccatcgccgc
     4381 ggacgagccc accagcggat cactctgccc cagggcttcc cgcagccgcc gattctcggc
     4441 cagggtgcgc gcgagctcga gggcgccgcg caccgccacc aacacgccct ccggcgtcag
     4501 cggtttttcc aggaactgaa aggcgccgag cttggtggcg cggacggcgt cggcgagcga
     4561 ggccttgccg ctcatcatca ccacgggaat gtcgggcagc cggtgcttga ggcgctcgag
     4621 ggtcgccagg ccgtcgagct ccccgggcat catcaagtcg agcatgatca cgtccggcgg
     4681 tgtctccgtc agggcggcga ggccggccgc gccattcggc gcctcgctgg tctcgaaccc
     4741 ctcaccctgg agcagggcac ccaccatccg gcggatgttc gattcatcgt cgatcagcag
     4801 gacgcgagag ctcatgatgg gaacgtgatg gtgaaggtgg cgcccccgcc gggcgtgtcg
     4861 ccgacggaca cgctcccgcc atgggcttcg atggtctggc gaaccagggc cagaccgagc
     4921 cccgtgccgt cagctttcgt ggtgaagtac ggctcaaaga gccgggcctt gatatccgcc
     4981 ggtatccccg ggccatggtc ggcaatggtc acctggaagc cgctgccgcc cgccggcacg
     5041 accaggacgt tgatccgccc ggtgccgccc atcgcctccg ccgcgttgcg caagagattc
     5101 gagaacgcgc gacgcagcgg ctcgtagtaa cccgacagga ctcgtcgggt ggcgggtccc
     5161 gagacctgga cccgcactcc gtcgggcacc ccggttcttc ccagctcctc gagcagctcc
     5221 accaggtcca cgtcactctt cggtccctcg ggcaggcggc cgaaatccgc aaattcgcgg
     5281 gcgagccgct cgaggcggtt ggtctccgcg cccatcactt cgacggccgt ggtcgcccgt
     5341 ccattcggcg caccttccgc gaccgcgagc gagcgttcga gctgatcgac cgcgatccgc
     5401 atcgaggtga gcgggttctt gatctcgtgg gccacccggc gggcgacttc ccggaaggcg
     5461 cggagccgct cggactcgag ctcgcgctcg cgcgcggttt cgagtgcggc ggccagttcc
     5521 cggaccgcct ggcggagcgc ctcgaactcg ggggcgccgc gctgtggagg gccacccggg
     5581 agcggctcgc gtcgcctgat cagtctggtc cacccgacga gctcgtcgat gggacgggag
     5641 agttggcggg agagatggcc ggcgacgcga accgcgacga agagcagcac ggcgccgagg
     5701 acgatgatga ccgcggcgaa cccggcggac gaatagttcc gaagggtctg ggcgcgctgg
     5761 gcgagcgtga gggaggcgga gatctgatcc aggtggtggt ccatcgcccg gcgctcgcga
     5821 ctggacaact gggtggtgtc caccgtcttg agcatggtgc gcgccgaggc cgccacctct
     5881 tccagggagg cccgggctcc cgccatcggt cccgaggccc gtacactgag cacgagcccg
     5941 gtcaccgcca tggcggtggg gatggccgtc atcgccacga ggatcgcgaa gatccggcgg
     6001 cggaagggga gtggcatatt ggtgaagcaa tctatggaat tagcaggggt gcgacaattc
     6061 gacacacggc gccaagcgag gagatccatg cgcagtcaca cgcactacct ctggttcaat
     6121 acgaagcagc gccaggagat catcgatatc accgatacgg tcgagaccca ggtggccgaa
     6181 tcgggggttc gcgaggggat ggtcctggta tcggcgatgc acatctcggc gtcggtcttc
     6241 gtgaacgacc atgaatcggg gctttggcac gacattctga agtggctgga ggggacgatc
     6301 gctccgtggg acccgccccg gtaccggcac aacgagacgg gggaggataa tgcggcggcg
     6361 cacctgcgtt ctctcaccgt ggggcacgag gtgatcgtgc ccatcaccgc cgggaagctc
     6421 gacctggggc cctggcagcg ggtgttttac ggcgaatggg acggccagcg gaaaaagcga
     6481 gtaatcatca aggtgttagg ggactagtaa caacacggca agtcgtttac aattaaaggg
     6541 ttccggcgga cggcgaccaa agcgtggtgg cgtcgcgcgc ccgggtcgga acgagggcat
     6601 gccgggccgc gcctcgcgcg ccacttccct gttgagtttc ccccattgcc gctgcgcatt
     6661 tctggcctgc ctcacgcgcg cattcgcgcc tacgcttttc gcctacgacg agcaagcggg
     6721 cctttcaaga aggacaattg atgcaacagc ctcacgcaca cctctcgcga ccggttcaca
     6781 aggaaccgcc cgcccacgct ccgaacgccg cactcctcat cgatttcgac aacgtcacca
     6841 tgggcatccg ctcggatctc cagaccgagc tccggcgcct gctccaatcc gaaatcatcc
     6901 gcggcaaggt ggccgtgcag cgggcctacg cggactggcg ccggtacccg cagtacatcg
     6961 tcccactgtc cgaatcttcc atcgatttga tctttgcgcc ggcctacggc tcgaacaaga
     7021 agaacgccac cgacatccgc ctcgcgatcg atgcgctcga gctggtgttc acccggcctg
     7081 agatcgggac gttcattctg ctctccggcg actcggactt ctcgtcgctg gtcctgaagc
     7141 tcaaggaata cgggaagtac gtgatcggcg tggggatccg ggaatcctcg tcggacctcc
     7201 tgatccagaa ctgcgacgag tattactcgt acaacgagct cgcgggcttc cagaaggaaa
     7261 gcgacatcgt gcacgagcgg tgggacgtgt ggaagctggt cgtcgaagca gtcaagcaga
     7321 tgaccgccca caacgatgtg atgcgctcgg accgcctgaa acaggtgatg caggagatcg
     7381 accccgcgtt cgacgagaag gacgccgggt tctcccggtt caccaagttc gtccaggagg
     7441 cggcacaccg gggtctcatc cacgtcaccc gtcgcgagaa tggccagttc gagatcgccc
     7501 ttggcggggc ggcctcccag gctccggcgg cgtcgccggg cgcgcccgcg gaacccgaag
     7561 cgcctgccga acgccgcagc tcattcgtcg gcgatgcgtt cggcctcttg aagcaggccc
     7621 tggggcaatc ccgtgatgag gagccggtgg atacgcccgc tctggaggag gcggctccga
     7681 aggctcatga cgaagggtcc tcgcaaccgc ctgctctcgg ggcgcggtcc tatcacgagg
     7741 aagaagacga gccacccatg ccccagagct cggcgccgcc cccgccgtac tccacaccgg
     7801 tggcgggcca cattccggct cgcagcgccc ggttccgtcg cggctccaag ggcggtccac
     7861 gccccatcaa cacgccggtg gccggctcgc cgcgggttgg gcccgtcgag accccggtgg
     7921 cttccggcaa tagtgattcc aactcgggga gtgagcgccg cgaacgcccg gagcggggag
     7981 atcgtccccg gggcggccgt ggccgcggtg cccggggcgg ctctcgcagc gagcacgccg
     8041 agggctcagg ggagcgtccg gagcggggtg aacgagggga gcgcggtggc cggagccgtc
     8101 gtggaggccg gggacgtggt cgcggcggga gtggcggcac tggagaaggg aaccgggagc
     8161 ggttggccgg ggagtctcct cgtgccgagg cgtctccagt ggcaccttct gctcccgcac
     8221 ctcatgcccc accccccgct ccgaagaagg ctccggagcc tgacacgtca ggcgagtcct
     8281 tctggacgcg ggtgaagcgg ggcctgaccg gcggaggaaa cgtctgaccg gacgtccctt
     8341 tcccgacgac ttctacgcgc ggccgactcc gttggtcgcg cgtaggcttt tggggcacct
     8401 tctcgtttcg accatcggtg gccgccgcac cgtgggccgg atcgtcgaga ccgaggcgta
     8461 cgtcggcccg gacgaccccg cctgtcacgc ctggaagggg cgccgctctc cgatggtcga
     8521 atcgctctac gggcctcccg ggagggcgta cgtctacttc acttacggca tgcactggtg
     8581 catgaacgcc gtcacgaggc gcgaggggtt tccctcggcg gtgctgcttc gtgcgctcga
     8641 gccactcgaa ggcctcgagg ccatgcggag gcggcggggg cgggggattc cggacggccg
     8701 gctctgtgcg gggcctgcca tgctctgcca agccctgggc gtcacggggc gacaggacgg
     8761 gaagtccctg gccgtgggct ccctacggtt gctccgggat cctcacgtgc cccggttcaa
     8821 ggtggcggtg ggtccccgca tcgggattac ccgcgccgtg gattggccgc tgcgcttctg
     8881 ggtcgccgat tcccggtgga cctcccgcta atcaaaaggg gccgacggac tgtcggcccc
     8941 tttctcactc tcagggagtt accggttcag ttctgctcgg tgatgtcttc ctttgaagaa
     9001 ccgagcgaat accggatgcc gccgaccaca ctgtaggtcg ggccatcgag catgaactgc
     9061 tgggcggagg aggtcacgct ggccaggccg tagagtccca gccggccgaa gttgatctgg
     9121 aggccgaaag cgccccaggc aaaggccttg ctggcctgat cgctggcggc ctgcgccgcg
     9181 aggctcgaat cggagaagct ggcgcaggtg gtggtggcgt cggcgcccga gcagtcgatg
     9241 gccgggttca ggatctgcat gatggagaag ccacccccga tcatgggctg aatgtgcccg
     9301 tcgatcggga tcgccatcac gccgatggag aggcgccgga cattcgagaa ggacacgttc
     9361 cgcacgcccg agccggagtt ggcgtcctgg atctgcgcga ccgcggagct ggtgaagaag
     9421 gcctgctcgt agctcaggta aagcgcggtc cgcttcccgg tgatcaacca gtgcccgccg
     9481 acgatcggat cgaaatgcag gggctggagg ttggtcttgt agatgaacgc accgccgccg
     9541 ccgccccagt accactggaa ggcgtggcta ccacctgtgg tttgggcgtt caggaggcgg
     9601 gggctcacga ggagcgccac cgcgagggca caaagggtca gacgcgacac cagcttcatc
     9661 ggacacctcc ctaagaggct ccgaaggtag gaatctagac tgttgcgcgc aagtaggtta
     9721 agccagccga tcgcgaaact actttaggtc catcacatca gtgtgttgac tgtagcgaca
     9781 ctgtcgttat ggtggcttat tctaaatcag ccccaactga ccgcccacct tcttgaaagc
     9841 ccccagcgcg aagtccaggt cgtcccgcga atgcgccgcg gagatctgac accgcacccg
     9901 cgcgtgcccc tgcggaacca ccgggtaccc gaagcccgtc acgaacactc cctcggccaa
     9961 cagcatctcg ctcatctgaa tcgcctttga tgtctcgcca agaatcacgg gcacgatcgg
    10021 cgtctcaccc ggcagtggct tgaagccaag ctcaccgagg cggccacgga agtactgcgc
    10081 gttctcccgg agacgcgtca cgagttccgg atgccgctcc gtgtactgca cggcggcgag
    10141 cgcgctcgcc gccacggtcg gcggcagcgc gttgctgaag agctgaggcc gcgaccgctg
    10201 ggtgaggtag tcggtcaatg aggccggacc ggccgtgaac ccgcctgccg cgcctccgag
    10261 agccttcccc agggtcgagg tgatgatgtc cacctgtccc agcatcccga aatgctcagc
    10321 ggtgccgcgt ccggtctttc ccagcacgcc ggtggcgtgg gagtcgtcca tggcgaccac
    10381 cgcgtcgtac ttcttggcca gcgccacgat ctccgggagg cgggcgatgt ccccttccat
    10441 cgagaaaatg ccgtcggtga agatcacgcg cagccgcgca cccgacgccg ccttcagccg
    10501 gtcctcgaga tcggccatgt ccgagtgctt gtacacggcc gtctggcact tcgtgatggc
    10561 cttcgccagc ctgatcgagt cgatgatcga cgcgtggttc agctggtccg agatcacgat
    10621 gtcctgctcg gtgagcaggg tggggcaaag gccttcgttc gcgttccagc agctcacgta
    10681 cgagagggat gattcgcatc cgacgaagcg ggccagcgcc tgctcgagct cccggtgcac
    10741 ggtaaaggtt ccgcagatga agcgcaccga acccgtcccc gcgccgtacc ggtccagggc
    10801 gtcccggccc gccttcacga cctcagggac gttgcagagg cccaggtagt tgttcgacga
    10861 cagaatcatg acgtccccgc gcccttccat cttcacgcgg gccccttgcg gcgagtcgag
    10921 gtagttcagg cgcttgtaga cgccgtcctt ctggaactgc tggagttcgg ctgcgaggcg
    10981 ggaggcgaga ctcatgagat ctccaggatc accttgccgg cgtccccgga ccggatggcc
    11041 gcgagggcgt cgtcgatttt ggcgagcggg aagcggtgtg taatgacggg gcgggggtcg
    11101 aagagtccgg tcgacaggaa gcggcgcatt tggtgccagg tctcgtacat cttgcgcccc
    11161 acgacgccgt agacggtgat ccccttgaaa atgatttcgt tcgcgaaatc gatcggcacc
    11221 tggtctttgg gtatgccgag gagctgcacg cgcccgccca ttcgcacggt ggcgaacgcc
    11281 tggtgcaggg cgctcggaat gccgctcatc tcgcagacga cgtcgactcc ctcgcctccg
    11341 gtggccgcga gcaccgcctt ggtcacgtcc tccttcttgg catcgatggt gacgtgcgcc
    11401 cccattttcg cggcgagggc gagccgcttc gggttcacgt cggaggcgat cactttggcg
    11461 gcgccggcgg cacgcgcgat gccgaccgcg aagcatccga tgggaccgca tccgaccacg
    11521 aacacggtgc tccccggaat gtcggccgtc aggaccgtgt ggaacgcatt ccccatcgga
    11581 tccatgatcg cgccgatttc agtcgggatc ccgtcgagcg tcagcacatt gctcgccggc
    11641 atcacgatgt agttggcgaa ggccccatcc cggtccaccc cgatgatccg cgtgttcttg
    11701 cagatgtgcc cgttgccggt ccggcactgg aggcagtgac cgcagatgat gtgaccttcg
    11761 gcggtgacca gctgacccgc cgagagctcg gtcacacccg gtccgacctt cacgatttca
    11821 ccggcgaact cgtgtccgag aatggtgggg ggatgcatgc ggccggaggc ccaggcatcc
    11881 cagtccgcga tgtggagatc ggtgccgcac accccggcgt gcctgacggc gatcaggact
    11941 tcgccggcgg cggggggcgc gggatcggga acggccttga cggtcagccc gggcgcgcgc
    12001 gtctctttga cgatggcttg catgggggcc aagttaaggg gtacccgaag gccgggaagg
    12061 ggtagtccca tgaggcgcat ttggtgtgca gtggccattg ccgcggcagt gacggtcggt
    12121 ccgttctccg cgacggctca agtccctgat accgcggtct cgtttcgcgg catcgtcatg
    12181 cgggacacgg ggtcggcctg ggcgctgttc attccggtac ccgcccaggt tcggaacatc
    12241 cgtttcaatc tcctccacct gtccaaggtg agcgggatcg accgggatga gaatcggtac
    12301 ggcgaggtga gtggtcacct ggtggcggat gacgcgggta gtccggtgct cgaagtggcc
    12361 aagctcaagc tgatgagcat tccgggcgaa gtggaacggt cggtgtcccg cagcttcacg
    12421 gaacacgccg aggtagcggc gtcaattctt ccaaatcagt tcgccttccc ggactccggt
    12481 gcgtcgacga gggtacggct caggccggtg atcgcctttg cgattaccaa ccactccgat
    12541 gcgccgatcg atttcagctt tcacgacaac aaactggtgt gtgcgtcggt gttgcccagc
    12601 aaccggaccc aggaaccgat ctggaccggc gcatggccgg tgggtccgta cctcaccagc
    12661 atccagatca atctcagctc gacggttcgc gaagcggtga cgctcccgga ttccgtgagg
    12721 ctcccgccgg ggcactacaa gttgagggtg tggctgtgcg aggcggctga ctacaacgcg
    12781 gtgacggtgt tcgacgtgat caaacgatag aaaaaacgaa ggtcagcgct ggcgatcgag
    12841 aggcaagtgc tggtgcgggt gatcctcacc ggcctcaccg tccgcgcacc atacgtaggc
    12901 ccagatcttc gccggcttga gcgcttcgcg ccccatccgg agaatgattc caatcggctc
    12961 gtcttccagg tacatgggga ggctggtcat gccgtcttca aaggcaagac gccggccaga
    13021 tgaagcagtt tacaagctat tgatttatag atagttacga agcagcaatg gaaggctatt
    13081 cgtcaatctg acacgcgtag ctatgtgtcg ctttgacgca ccggcttcgt agcgacgaac
    13141 ccctctcccc gcccgcgccg cttccggcgc tccacctgcc actcgccgta caaactctgg
    13201 accgcatcgg gcgcgagggg caggaccccc ggtccatctg gatcgacgct caggatgacg
    13261 tgaatgcctc ctgagcgtgt gcaggcctga agcctgcgga ccaggttctc gcgatccccg
    13321 gcatccgcgg tcgccagggc gttcggttcg atgacggtca gtgcgggacg caagtcggga
    13381 agccacccgc cgaaattcac gacgagcgcc tggaagcgcc cggcgagctg ttcggacacg
    13441 gcgcgtcctt ccgcggactc caccgcgccg agttcctggt cgagcaggaa gacttccgcg
    13501 tcgtgcgcgg cgacgaagag cgcgtggggc aacgccgcgg cgcccaccac gagcaccagg
    13561 tctcccgctt ccagccgctt cacgccctgc gcgacgggat cgctcgactc gagcccccag
    13621 tgggtcggct cgcgctgagc ggtgcgcaag gcgcggtggt tctgcgacca gcgccggaac
    13681 gagggcaact tgagtcggcg tgagatgatc cggtcgacgt gttcgaggag gagcacttcg
    13741 gtgaggaggt actgaccttc ccccgactcg agctctcgca ccgcctcgtc gccgatgcgc
    13801 agcagctcct cacgcgggac ggtgttcttg tactcctcga tccgctggag caggaattcc
    13861 tgatactcct gcttctgcga tcgcggcggg cgccgggtgg gcccggtggc gtggggagaa
    13921 ccctgcgcga cggtgttcag catggctcga tgtgcaccgt gactccgtga aaatcgagct
    13981 gctgcttcaa ccgggcttcg acctggtccg cgagctggtg ggcctgggcg acggtggcgc
    14041 cgccgtccac ggaaatcgtc agctccgcat agcgcccatt ggcgctgccg cgtgaccgga
    14101 tatcatacgc gtcacagact cccggaacgc cccgggcaca ctcggcgatg cgctccggcg
    14161 ggtggacgcg ctcgtcgacc agcaccggaa ccgtccgtat cagcacccgg taggccaccg
    14221 acaggatggc accgaccacg aggatcgcga tgaccggatc ggcccaccac cagccacgcc
    14281 gcgccaggag cagcgcgacg atgactcccg cggtgatgag gacgtcggca ctcgtgtgtg
    14341 cggcgtcggc gagcaggatc tcgctctgca gttcggtcgc gcgccgtcgc tcgtaccgag
    14401 cgacgcccgc gttcaccagg agggtcgccg cgaggacgcc gacctgggtg ttggtcacgg
    14461 gcagggggtg gccgccggag gtcaggtgat tcaccgcacc ccgcagcagc tcgaacgagg
    14521 agacgcagag aaaccccacc agggccaggg cgcccagcgt ttcgaatttc ccatgcccgt
    14581 agggatgctc ctcatcgggg gctttcgccg cgaccgcggt cacgatcaag gcgaggacgt
    14641 tgttggccga atcgatggcc gaatctagag cgctccccaa gacccccagg gatccagacg
    14701 ccagaccgac gccgactttc accaggacca cggccaggtt ggcgagcagg atgccggtca
    14761 ggacccggcg aacggcagtg ggattcggca cctgagcaac cttgtaagcg gggcgcgcca
    14821 attcaagctt gacaataatt agacatttac ctaaatatct catcaatgcg ggatcacttg
    14881 ttcaaggccc tggccgaccc cacacgacgg aagatcctcg agctcctggc cgcgaaggac
    14941 cgcacggccg gcgagttggc ggaggaattt ccgatcgcgt ttgcctcggt gtcccaccac
    15001 ctcggggtcc tcaagaacgc cggactcgtc gcggtggagc gggaggggca gcacatgcgg
    15061 taccggctga acacgaccgt ctttcaggaa atcgcccggt atctcgtgaa aacgttctcg
    15121 ggaggagctg atgcgtagcc gatggttcgg cttggtaatc gccgcgctgg ctctggtggc
    15181 cacgtggctc gcgtgggaca aacttcctcc gcacatggcg acgcactggg acgccagtgg
    15241 ccacatcaac ggctactcgt cccgggcgtt cggcgcgttg ctggcgccgg cgatgatgat
    15301 cgggttgacc ctgctgttcc aggtcctgcc ggcgctcgac ccccgccgcg ccaactacgc
    15361 caagttcgcc gacacgtact gggtgatcgc gaatgccgtc atcctgttcc tgggcatcgt
    15421 ccatctcatg gtgctcgcct acggcctggg gtacggggtg tccatgaccc gcgtgcttcc
    15481 cctcggcctc ggcgtcctct tcatcgctct ggggaatgtc cttccccgcg tggaaccgaa
    15541 ctggttcgta ggcatccgga ctccctggac cttgtcgtcg gacaccgtgt ggcggaagac
    15601 gcaccgggcc ggcgggttcg tattcctgct cggtggatgc gcgctcctga tcgaaggcat
    15661 cacaccactc ggcaacgtcc tgccggtcat gatcgggacc atcgtcgtga cggccctgac
    15721 gccggtggtg cagtcctacc tgttgtggaa gggagagcag ggtgatcgcg cttagtctcg
    15781 cattgctcca ggcgccgcag cagaaggccg ataccgtggc cccgggggcc gcggtcgaga
    15841 gcccgttcgt cgcgacgtcg tcggggtgga cgctgtacgg caccctcacc gttcccaagg
    15901 ccgcggccgg acccgtgccg gtggtcctca tcgtggcggg ctccggtccc accgaccgga
    15961 acgccaattc tcctgccgga atccgcagca acacctacgc ccagctcgcg tggcgcctgg
    16021 cggagcgggg catcgcttcg ctgcggtacg acaaacgggg catcgccgct tcccggggcg
    16081 ccatggattc cgccgtcgtg gcgcagacca ccatgcagga ctacgcgaac gacgtccgga
    16141 acctggcgga ctccctccac acggatcacc gcttttcgag agtgatcctg gtgggccaca
    16201 gcgaggggag cgcgctctcc atcatggccg cgcacgacgg cgcgccggtg gcgggagtgg
    16261 tctcgatggc gggaatggga cgtccgttcg cgacagtcct gcgggagcag ttggcggccc
    16321 agctcgattc gaacaccctg aggatgtacg acacatcgat gacggcctat ctcgcggggc
    16381 gcgacccgcc ggtgcccagc tacctcgcat cactcttcgt ccccgcgctt cggaactacc
    16441 tccggtcgat gatggcgttc gacgcgaagg aagtcggaca gctgcactgc cccgtcctgg
    16501 tcctgcaggg cgactacgac cttcaggtca cagtggcgga tgcagaggcg ctccacgcgg
    16561 cagatccgaa atcgacgctc gtgatcttcg cgggggacac gcacatgttc aaaatcgcgg
    16621 cgagcaagga tcggatggcg gaagtgccga cgtacgtgga tccgaccgtg ccgatcgatc
    16681 ccaaggtggt tgctacgatt tcggactgga tcggaaacat tcggtaggcg tcacccccag
    16741 cgccttgcgg aacatcgaaa tgaaggcgct gggggtggcg tacccggctt cgagcgcggc
    16801 gtgactcacc ttcgcacctt cggccagaag ccgcattccg tgcatcagcc gcgcctgctg
    16861 ccgccagcgg cccaatccca tccctgcctc cgacacgaac aggcgctcaa tggtgcgtcg
    16921 gctggcgccg gcgtattggc agagatcctc aagggccccg ggctcgggat aggctaggat
    16981 cgcatgggcc accttctgtg ccctggcatc ccgtggatag gggagctgca agggaaccat
    17041 ctggatctcg cgcagttgat caatgatcaa cgcgatcagg tgccggtggc ggctgatccg
    17101 tcggtgcagt tccccaaccc gacatgcctc aaggatcagt tcttcgagga gcggcgtgac
    17161 gctgacgacg ctcaactcca ttggcgggac ggttcgcgag cgcaagtaga gtgtgcgcat
    17221 ctcgacgacg cccgacgtgg tgatttcatg tgacacaccg gcgggcaccc agacggcgcg
    17281 acgcgggggg actacccagg tcgtggctcc cgcggccacc gtcatcaccc cccggctcgc
    17341 gtggacgagt tgatcccggt cgtggtagtg cgccggtatg aggtaccgcg ccggataatc
    17401 tcgagtccac gtccggacgt cggggccagt ggtccccaac ggctcaaaac ccgtttgtcg
    17461 caatttcgac attagttgac agcatatcga aagcgcgaaa gtggcgcaac ggcctacgta
    17521 cccacatacc agacgccgag gaatgtcccg tgcatggtta cttccggatc gggatccagc
    17581 agatctggga gaagaacaac cagatcgtgt tcgagacgat ctatgcccgc cgcaggagcg
    17641 cgcctttgag gtagccggtc tcggggatgg tgaggatctc ggggtggtct gacgactgac
    17701 ccgtgaagcg caggacttcg agccttcggc cggagtcctg cgccgcttcc aggagcatgg
    17761 cctcgaagtc gcgacggtgc acgtgatagg agcaggagca cgtaaagagc acgccgccag
    17821 gcgcgaggca ccgcatcgcg cgaagattga tctccttgta gcccgcgagt gcgcggtcga
    17881 cactcgcttt agtcttggca aacgccggcg gatcgagagc aatcgtgtca aaggcctcac
    17941 cgcgcccctc gaactcccgc aagagatcaa atacgttggc ttccttccag gtcacattcc
    18001 caaacccatt caacgcggcg ttctttgccc cccggaccag agcctcggcg ctcgagtcca
    18061 ccgccagtac cgagcccgcc accttcgcca ggtgaatcgt gaaggagccg tggtaggcga
    18121 agaaatcggc ggcgcggccg cccggcctcg cgtgctcggc cagcattacg cggttctccc
    18181 gctgatcgag aaacccgccc gtcttctggc cctgccacgg tgccgccaga tacctcacgc
    18241 ccgactcagt gacctcgacg ctctccggca ccgtaccgga aagcagggac acttcgagtg
    18301 gaagcccttc gtgccgacgt accgcggcgt catttcggag caggatcccc tctggatgga
    18361 ggacatcctg tatggcggcg ccgatgtgct cgcgagccga ttcaacgccc gcggagagaa
    18421 gctgcgcaac ggcatacggg ccgtaccggt cgatgacgag tgacggcagt ccgtcgccct
    18481 caccatgcac cacccgccac gccgtggcgt cgatgccggt gcgcttggcc gccgcggccg
    18541 caatgcgcga ccgccaccac tcccggtcga tctcgacgtc ctcggtggtc aggaggcgca
    18601 gcctgatctc cgacttcggt gagtagagcg cccgggcgag gtacttgccc ttccggtcgg
    18661 tgacccggac gaccccagcc tggtcgcccg gctcctcggc aacatcggac cggtagaccc
    18721 agggatgccc ggcacgccac cgcgcggcgc ccttcgcggt gatcgcgacg ttcatgtcga
    18781 ccgatcgggg cgcaaagcgg cggccccccg gagatagata tgtttgagcg cgcccggctt
    18841 ccgcgccgtg tagaaatgaa ggaggacacg gatgcagaga ggcacgcccc cgggcaccgg
    18901 cacttcctgc gccccgagga gcggtgcgcc ggtccagccc agattccggg ccgcgcgcgc
    18961 cgggtattcg gcggtgaggt cgggcgtgga cgtgaagatg gcgctctcga tgtcctcagg
    19021 ggcgaaggcg ttcgccttct gcagggcggc gagcagctcg gccgtcgcat cgagaatggc
    19081 gtcggaggtg ttggcggtcg ccgtcgtggc gccgcgcaac ccccgcagaa tccgggtggt
    19141 caatcgatgc tcgttgatcg ggtgcgcttg atggtgctca cggacgcctc gcttctcaag
    19201 gggcggaatg tggtggaggt gtgccgccgg gccgtcgccg gtggtgcgac gtccatccag
    19261 gtccgctgga agcccgcccc cgcgcgcgaa gtggcggacg tcgcgcgggc tctcgtgggc
    19321 gccctctccg tgccggtgct cgtcaacgac cgcctcgacg tcgccctggc ggttggtgcc
    19381 gcgggcgtgc acctgggaca ggacgacgtc cccgtggcgg ccctcgccgc acacctccca
    19441 cccggtttca tcgtcggcat ctccgtcggc actcccatgg aggcgagtga cgccgacctc
    19501 cgggccgact actggagcat cggtccctgt tacgccacgt ccaccaaatc ggatgccggt
    19561 gcccccctgg gcccggaggg tttcgggagg ctcgcccggc tcgcgccggc aggccttccc
    19621 gtgatcggga tcgggggcat taccaaggac aacgccgccg cgatcaggcg agccggcgcg
    19681 gcgggcgtcg cggttatcgc gtcggtgctg gcgtcggatg acccggagcg cgcttcgcgc
    19741 gagctgcggc tttcagtcgc ttgaccttct ggattgcggc aaggtgctcc gcgtaggtca
    19801 ccgagaagat gtgcttcccg tccggttggg ccacgaaata gagatagggc acctcggccg
    19861 gatgaatggt cgcgtccaga ctcgcatgcc cgggttgcgc gattggtccc ggtggcagtc
    19921 ccgcgtgcag gtaggtgttg tacggtgacc ggatcaggag attcttttcg aaaacccgct
    19981 tgagccggtg gccatacgcg tagatcaccg tcgggtccgc ttcgagcttc atcccccgcc
    20041 gcagccgatt caagtacacc gccgccacat agggccggtc cgggtcatac cgcacctcgg
    20101 cttcgacaat cgacgccacc gtgacgatcg cctgccgtga caggtgctgc gagtcgagcg
    20161 tcgccgtcca ggtcgagtcc cactggccca gaaactcgcg aaccatcgcg cgcaccaggt
    20221 cgcgcgcgcc gatcttggcg ggcacgagat acgtggtggg gtagaggtac ccctccaccg
    20281 tcggtgcatt ggacggcaga ccgagctcgg acttgagatc cgggtccgct atggccagca
    20341 ggaaggaatc gcgggggatt ccgagccggc tccgggccag gtccgcgact tccgtggcca
    20401 tcaacccttc cggggtggtg aaatgcacca gcgcgccacg cccggtcgac agcgcttcgg
    20461 tgatcacccg ccacgtctca tcttcatgga aggcgtaggt cccgctcttg agattcgacc
    20521 ggagaccgac gaagcgggcg tagatccgga accagaacgg atgggcgatg acaccgcgcg
    20581 ccgaaaggga gtccatcgcg acatcgagcg tcgcaccgtg gggaacggtc acggcgatct
    20641 gggggccacg ggccggcgtg gtggagcacg cggcgagcag gaccgtcagg acctgggcga
    20701 tgcgcttctt cacacggcac tcccgcgcag ggcgtcgagg tagtgctgca ggagcagcgt
    20761 cgccgcgagg gcgtcgacat cgcccttccg cccccgggtc ttgccgccca tctctcgcac
    20821 ggcgcccagc acgcgggcgg tggtgaagcg ctcatcccag agggcgacgg ggagtccggt
    20881 gcgccgcgcg atgtcgtcag ccagctgccg ggcggccgtc gcggcatcgc cttcggcacc
    20941 cgctgcgtcg aggggaagtc cgaccacgat gccggtcacc ccgtgggtgg caaggtgcgc
    21001 ctgcagcgcc gccatgggga agcgcttgcc gggccggcga gtgagggtgt cgaggggttg
    21061 ggccagcgtt cgggattcgt cggagagggc gaggccgatc cgcttctcgc cccagtcgac
    21121 gcccaacagc cggcccatcc ccccgctcac ccttgggcca tcgtcgcgaa gaactcggtg
    21181 ttggtcttgg tgcgcttcat ccgctccagc aggaactcga tcgcttccac gggcggcatg
    21241 tccgccagga agttgcggag gaggtagacc ttgttgagct cgtccttggc caggaggagc
    21301 tcttctttcc gggtggaggt ccgttggacg tcgatggcgg ggtagacccg gcggtcggcg
    21361 atccggcggt cgagaatcac ttccatgttg ccggtgccct tgaactcttc gaagatgact
    21421 tcgtccatcc gcgagcccgt gtcgatcagg gcggtggcca gaatcgtgag cgaccccccg
    21481 ccttcgatgt tgcgcgcggc gccgaagaac cgcttgggct tctggagcgc attggcgtcc
    21541 acaccgcccg agaggatctt gccggagtgc gggaccacca cgttgtgggc ccggcccagt
    21601 cgggtgatgg agtcgagcag gatcacgacg tccttgccgt gctcgaccat gcgcttcgcc
    21661 ttctcgatca ccatgtccgc cacctgcacg tggcggtcgg cgggctcgtc gaaggtcgac
    21721 gagatgacct ccgcgccctt gacgttctct tccatgtcgg tcacttcttc gggccgctcg
    21781 tcgatgagca gcacgatgag gtagacctcg gggtggttct cgatgatgga gttggcgagt
    21841 ttttgcatca ggatggtctt gccggccttc ggcggcgaga cgatcagccc gcgttggccc
    21901 ttgccgatcg gacacaggat gtccatcacc cgcatcgaga ggtcgccgtc cttccgctcg
    21961 aggcgaatcc gctcttcggg ataccggggc ctcaggttgt cgaacgcgac ccggtgcttg
    22021 gccttttcgg gctcctcgcc gttgacgttc tcgaccttga gcagcgcgag gtaccgctcg
    22081 ccttccttgg gaggacgaac ctgacccagg atggtgtcgc cggtgcggag gtcgaagcgc
    22141 ttgatctgcg aaggcgagac gtagatgtcg tcggggccgt acaggtaatt ccagtcgggg
    22201 ctgcgaagga agccgtatcc ttccggcaga atctccagta ccccttcgcc gcgcaaaacc
    22261 gtgtcggtgt cgagcagtga ttgctcgatg cggaagatca ggtcttgctt tcgcatcccc
    22321 gagtagtttt gaatgttcag actctcggcc atcgcgtgca gctcggcgac ggattttctc
    22381 ttgagttcag cgatgtccac gtgtgcttcg gctccggtaa gcgatgtacg gcggcgggaa
    22441 ccccctgcaa tcgggggagt gaacgcggtt tgtatgttgt aaaagagggt gcttgggtat
    22501 ctgtaggcgt gccagtgact tatagtagag gccccggccc gaggcgtcaa gggtgcccat
    22561 gaacctgatg tcgttcgtcg agcgggcgga gaccacgtca ctgcccgatc gtgaagtcgt
    22621 tgatgcgctt gtacatccgg cgcatgcggc gccgtccccc cagctgcggg acgcgctcga
    22681 gacgtggcgc ggtccttact actggtccga cgaatcggac ggacgccacc tcattctgac
    22741 gttggtcagg ccgcgccgcg gcgaacgctg gtggctgcac gccgcgctct ttgtgctgac
    22801 gttcgcgagt gtcacgctgt ccggtgccgt actcgaaggc cgccttcctt attacggtgt
    22861 gctcgggctc ctcacctcgc tcgcgcaccc gagcgcccgc ttctggagta cctgggcatc
    22921 cgggctcccg ttctcgatcc ctctcctggg cattctgctc gcccacgagc tcggccatta
    22981 cgtcgcggcc cggcggcacg gtgtgaacgc gtcgccgcct ttcttcatcc ccgcgcctct
    23041 gatctccatc atcggcagcc tcggtgcatt catccggata cgcaccattc tgagtgaccg
    23101 gcgtcaactg ttggacatcg gagtcgcggg tccgttcgcc ggcttcgtgg tcgcgctgcc
    23161 ggtgctgatg gtcggcttgt cgcagtcgca tacgcatccg ctgctcgcac ccgggtcgaa
    23221 cctcctgctc gtcgtcggcc acgatatcgt gcctatcggc tcgtcgctcc tcaccagcgg
    23281 cgtgcgggcg ctcgtccttg ggggctccgg gggcctggtg ctgtcgcccc tcgcgttcgc
    23341 cggatggatc gggatgttcg tcacgatgct gaacctcctc cccctcgcgc agctcgacgg
    23401 cggccacgcg ttgtacgcgg cggcgccgag gctccacggg aggctggcgc tcctgttctg
    23461 gggggcgctg gccgtgctgg ggtggttcac ctggcaggg
//
LOCUS       DASUBP010000049         3761 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1077774, whole genome shotgun sequence.
ACCESSION   DASUBP010000049 DASUBP010000000
VERSION     DASUBP010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1077774"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(82..531)
                     /locus_tag="VFU75_02260"
     CDS             complement(82..531)
                     /locus_tag="VFU75_02260"
                     /inference="COORDINATES: protein motif:HMM:NF019575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU5465680.1"
                     /translation="MKNAGFTMLELMVVVILIAVLTTLALPKLTTAAQKANIRSTRLE
                     LSTMVTKARAAAIQRGCVDSLHINSTQAWVTACKVSGTGRDTVGPVDRVGNRFGVTLS
                     SSTTNYGFDPRGLLVGFAGGTIVITGTGGNAGFSDSVVINNVGKVTH"
     gene            722..1594
                     /locus_tag="VFU75_02265"
     CDS             722..1594
                     /locus_tag="VFU75_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465681.1"
                     /translation="MSRCTRLGSALVASVFLASAAYAQVSYGVSGGAAKLNDLQKDQT
                     LTATLQWQVRPWIGFSVAPGYIHAENDSGGVAYTSTGFGDLPIGADLSYSFDDAPWSP
                     DIGTSLTMTLPTGLQRCGLGTGNTGVGVDLGVGVQPIDPLHVAVTTSRDLTGQGAASL
                     LDPPHATSLSFEGSYDLSPRLTAGLSFGGDFGTPDSGETLPRTIGGGATIHLPHSPFA
                     FAIGGSHSLTSGPPRWALTIGFGTAFAGTDPVGINSPLHLLKKSLNGSVNRGSGRGGV
                     GGKGKSGPVTTSCS"
     gene            complement(1660..2595)
                     /locus_tag="VFU75_02270"
     CDS             complement(1660..2595)
                     /locus_tag="VFU75_02270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5465682.1"
                     /translation="MTTNPRVSVGIPLFNEEQGVPELIRRVAAVLDGLPGGPHEIVCV
                     DDGSRDGTVRVLEQLTAKERRLKVVAFSRNFGHQTALTAALDHVAGDVVVVMDGDLQD
                     PPEEIPRFLKHYAEGYDVVYAKRVGRKESWWLRLCYFGFYRLMSSLSTLPLPLDAGDF
                     SLMSRRVVEVLKSSPEHHRYLRGLRAWAGFKQLGVEVERPARFAGESQYSLWALMRLA
                     ANGIFAFSTIPLKAAALLGTLAIGLAMLYVPWALYAKFILKQSPQGFTTLIVAIVFLS
                     GVNLFFLGIIGAYMGRVYEEVKRRPLYVVDRVIGG"
     gene            complement(2592..3191)
                     /locus_tag="VFU75_02275"
     CDS             complement(2592..3191)
                     /locus_tag="VFU75_02275"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5465683.1"
                     /translation="MKITDKLLEQPIVYRAWMAPFAEAKLAPMRAHTDFSKLKRVLDV
                     GCGPGTNAPHFAQCDYLGVDINPQYVADATKRYKRKFLAADITQYQVSGDPFDCILVN
                     SFLHHVDLPSTHRILAHLPSLLPPDGFVHIIELTLPPNASPSRLLAKLDRGDYPRPLE
                     EWRGIFREHFEEVVFEPYSVGMAGVTLWKFVYFKGRKRP"
     gene            complement(3188..>3761)
                     /locus_tag="VFU75_02280"
     CDS             complement(3188..>3761)
                     /locus_tag="VFU75_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465684.1"
                     /translation="SAPIYFLYIAPLGVVTAAAVLEIQFEGAAAWPVFGGALLLFAIL
                     FPVRWIRPGFTSDLGVRYTRVPMTALLPGRAQLWITPAEAAEYTGMTALVAAHARGGY
                     VYATPDCPEVNVLTGTRNPTRTLFDFFDDPVNRTARILAQLDSTGVTTVLLNSRPGFS
                     GPVPSDLAAALAVRYPHAQSIGRFEVRWRE"
BASE COUNT          697 a         1281 c         1141 g          642 t
ORIGIN      
        1 cgctcgcgag ccccaggagg cccacgcaca ggatcacgac cgcgatcatg atctcgacga
       61 tggtaaagcc gcgacggttc attagtgggt caccttgccg acgttgttga tcaccacaga
      121 atccgagaac cccgcattgc caccggtccc ggtgatgacg atggtgccac cggcgaaccc
      181 caccagcaat ccccggggat cgaatccata gttcgtcgtc gaagaagaga gcgtcacgcc
      241 gaaccggttg ccgacccgat cgactgggcc gaccgtgtcc cggcccgtac ccgagacctt
      301 gcatgccgtc acccacgcct gcgtactgtt gatgtggaga ctgtccacac acccccgctg
      361 aatcgcggcg gcgcgggctt tggtcaccat cgtcgacagc tcgagtcgcg tcgagcggat
      421 gttggccttc tgagcggcag tggtgagctt cggcagtgcc agagtcgtca gcacggcgat
      481 caggatcacc acgaccatga gctcgagcat cgtaaagccg gcatttttca tcggtgacgt
      541 cccctccatt ccgggggtct gtgttggcga taagtgtgcc atatacttaa gcctatatgt
      601 gacatggaca tgccctagtc cagggccctg gatcaccgcc cgcaaatcgc ggaaacagga
      661 tactgtagtg cgtttttttg catagcgtat cgcgaagcta acccccaaat cggagcaccc
      721 agtgagtcgc tgcactcgtc tcggatccgc cctcgtcgca tcggtattcc tggcctccgc
      781 ggcctacgcg caggtctcgt acggcgtctc aggcggagcc gccaagctca acgacctcca
      841 gaaagaccag accctcaccg ccaccctgca gtggcaggtc cggccctgga tcgggttctc
      901 cgtcgccccc ggctacatcc acgccgagaa cgactccggg ggagtcgcgt acaccagcac
      961 ggggttcggt gaccttccga tcggcgcgga tctctcgtac agcttcgacg acgcaccctg
     1021 gtcccccgac atcggtacgt ccctcaccat gaccttgccc accggcctcc agcgctgcgg
     1081 actcggcacc ggcaacaccg gcgtcggagt cgacctgggg gtcggcgtcc agccgatcga
     1141 tcccctccac gtcgcggtca ccaccagcag ggacctcacc ggtcaggggg ccgcctcgct
     1201 gctcgatccc ccgcacgcca cgtcgctctc gttcgagggc tcctacgacc tttcaccgcg
     1261 cttgaccgcg ggactctcgt tcgggggaga tttcggaaca cccgactccg gtgagacgct
     1321 gccgcgaacc atcggcggag gtgccaccat tcaccttccc cacagcccgt tcgccttcgc
     1381 gatcggcggg agccacagcc tcacctcggg accgccgcgc tgggcgctca ccatcgggtt
     1441 cggcaccgca ttcgcgggca ccgatccggt cggcatcaac tcccccctgc acctgttgaa
     1501 gaagtctctg aacggatccg tgaaccgggg tagcggcagg ggcggtgtgg gcgggaaggg
     1561 caagagcgga ccggtcacga cgtcctgcag ctgaccctgc gattctcctc gacgattttc
     1621 ctcgacgtct ttccttgacg tcgttgttgt tgttgtttct cagccgccga tcactctgtc
     1681 tacgacatag agtggtcggc gcttcacttc ctcgtacacc cgccccatgt aggcgccgat
     1741 gatgccgagg aagaagagat tcacgcccga caggaacacg atcgccacga tgagcgtcgt
     1801 gaatccctgc gggctctgct tgaggatgaa cttcgcgtag agcgcccagg gcacgtagag
     1861 catcgccaaa ccgatggcga gggtgcccag gagcgctgcg gctttcaggg ggatggtcga
     1921 gaatgcaaag atcccgttcg ccgcgagtcg catcagcgcc cagaggctgt actggctttc
     1981 ccccgcaaag cgcgccggac gctcgacctc cacgccgagc tgcttgaagc ccgcccaggc
     2041 cctgaggcct cggagatagc ggtgatgctc cggggagctc ttcagtacct ccactacgcg
     2101 acgcgacatc agagagaagt cgcccgcgtc gagcgggagg gggagcgtgg aaagtgagga
     2161 catgagccga tagaaaccga agtaacacaa ccggagccac cacgattcct tccgccccac
     2221 ccgcttcgcg tacacgacgt catagccttc ggcgtaatgt ttcagaaacc ggggaatctc
     2281 ttcaggcgga tcctggagat ccccgtccat caccaccacg acgtcaccgg cgacatgatc
     2341 gagcgccgcc gtgagtgccg tctggtggcc gaagttccgg gagaacgcca ccaccttgag
     2401 gcgccgctcc ttcgccgtca gctgctccag cacgcgcacc gtgccgtccc ggctcccgtc
     2461 gtccacacaa acgatctcat gaggtccccc cggcaggccg tccagcaccg cagccacccg
     2521 ccggatgagc tccggtaccc cctgctcctc attgaaaaga ggaatcccca ccgagactcg
     2581 cgggttggtg gtcacgggcg cttccgtcct ttgaagtaga cgaatttcca gagcgtgacc
     2641 ccggccatcc ccacggaata cggctcgaag acgacttcct cgaaatgctc ccggaagatc
     2701 ccgcgccatt cttcgagcgg ccggggataa tccccccggt ccagtttggc gagaagtctt
     2761 gatggcgacg cgttcggcgg cagggtgagc tcgatgatgt gcacgaaccc gtccggcggc
     2821 agcagcgaag gcaggtgggc caggatccgg tgcgtgctgg gcaggtcgac gtggtgcagg
     2881 aaactgttca ccaggatgca gtcgaacggg tccccgctca cctgatactg ggtgatatcc
     2941 gccgccagga acttgcgctt gtagcgcttg gtcgcgtccg ccacgtactg gggattgatg
     3001 tccacaccca gataatcgca ttgggcgaag tgcggggcgt tggtccccgg cccgcaaccc
     3061 acatccagaa cccgcttgag cttggagaaa tccgtatgcg cccgcatcgg cgccagcttc
     3121 gcctcggcga agggcgccat ccaggcccgg tacacgatgg gctgctcgag cagcttgtca
     3181 gtgatcttca ttcccgccac cgcacctcga accgcccgat ggattgcgcg tgaggatacc
     3241 gcaccgccag cgcggccgcg aggtcgctgg ggacaggacc cgagaaaccg gggcggctgt
     3301 tgaggaggac cgtcgtcaca ccggtcgagt cgagctgagc gagaatgcgc gccgtgcggt
     3361 tcaccggatc gtcgaaaaag tcgaacagcg ttcgcgtcgg attcctggtg ccggtgagca
     3421 cgttcacttc agggcagtcg ggcgtcgcat agacgtatcc tccacgggca tgcgccgcga
     3481 ccagggcggt catccccgtg tactcggccg cctcagcggg agtgatccac agttgcgccc
     3541 gtcccggcaa gagcgccgtc atggggaccc gcgtgtagcg tacccccagg tcgctcgtga
     3601 acccaggacg gatccagcgc accgggaaca ggatggcgaa cagcagcaac gcgcccccga
     3661 acaccggcca cgccgccgcc ccttcgaact ggatctcgag taccgccgcg gccgtcacca
     3721 cgccgagcgg cgcgatatac aggaagtaga tcggggcact g
//
LOCUS       DASUBP010000050         5608 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_164791, whole genome shotgun sequence.
ACCESSION   DASUBP010000050 DASUBP010000000
VERSION     DASUBP010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164791"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFU75_02285"
     CDS             complement(<1..185)
                     /locus_tag="VFU75_02285"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465685.1"
                     /translation="MSIRVVIADKLGDAGIALLASSPEIHVVNLAGKSREELEAALTT
                     AEALIVRSETKVTAEMIA"
     gene            complement(182..1270)
                     /locus_tag="VFU75_02290"
     CDS             complement(182..1270)
                     /locus_tag="VFU75_02290"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HEU5465686.1"
                     /translation="MSLPTPAFGKFFLPGPTDVHPDVLAAMYRPMIGHRSSAMEDLLK
                     RCAPKLQQLARTSRTVMVGTTSATGFMEMAVRSGIRKKAISIVNGSFSERFADLVKAC
                     DKECVRLEVPLGGFVEPDQLRDALKKNPGVDAVTLAHSETSTGVLQDVAALSKVVHEF
                     PDVLFLVDAVTSMAGSPVETDAWDLDYVFTGSQKALALPPGLAFGVASPRMLERARTL
                     PSRGLYFDLVTFEKASAKYSPTNTPALSLFFALDVQLERIDAEGGIEARWKRHDDMRK
                     TTEKWVQKGGLSYLPPEGRRSWTVSCIKMPNGKISKDLVGAVKKEGWVIGTGYGGVKD
                     STFRIGHMGDHSVASLENLLALIQRILG"
     gene            complement(1267..1905)
                     /locus_tag="VFU75_02295"
     CDS             complement(1267..1905)
                     /locus_tag="VFU75_02295"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HEU5465687.1"
                     /translation="MARVIFWDVDTQYDFMRADGKLYVPDAEKVIPNLKRLTDYAHQN
                     DIRVVASADDHSPGDKELSDHPNWRDTFPPHCMHGTPAQKKIPETALKNPMVIEPEMR
                     DPVELGNALRAHKGDLLFHKHWFDVFTNANVVPALTVLSPDDIVLYGVALDVCNRYAI
                     EGLLTHRLHTRLFAVTDAMKPIDASAAEHLLKEWGEEGVRLVKTDEVLGGIA"
     gene            complement(2061..3098)
                     /locus_tag="VFU75_02300"
     CDS             complement(2061..3098)
                     /locus_tag="VFU75_02300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465688.1"
                     /translation="MASDIHKKLVFGALGCGGLLVVFGGIGLLLTKDQLFKGSEAQQT
                     YTSDDGIAAWDTLSGFAITDSLVPPSLPPWSLSGATSPGTLVLGASRSGNNLPAFGDT
                     FPAGSKERKGWEESGASVTISRPTDKNHLSRSMVLVSPPAGFRLFQARVLFAAAETWK
                     GDGDLPDARMAMDSALNLARGMQQSGALERLLVGARIERDAMDVLSRDTTLAGGKAAA
                     ARAGERIAPLDRFVTRLRRVDRLLTIAGSDPGNARYLGDWVADTALPLAIRTATVRAI
                     GLGWVFSATEPGLGIDTSRTVTLQKLRKASTSDSVTVAIDHVIHQGDTFTSRIRVSTA
                     YRAERMFLDWR"
     gene            complement(3108..3602)
                     /locus_tag="VFU75_02305"
     CDS             complement(3108..3602)
                     /locus_tag="VFU75_02305"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5465689.1"
                     /translation="MSALPLPLLHPALEIRPASLADVPALEKLMAPFIATGDLLPRSN
                     YDLCRHIKEYVIAGTPEGEVIGCGSLKVYSQTLGEVAGLAVADGWQGAGVGRAIVEGL
                     LEDARELGLAETFALTRKPDFFSRLGFARTEKTRFPLKVWADCARCPRQSCCDEIAVT
                     RALG"
     gene            complement(3599..4762)
                     /locus_tag="VFU75_02310"
     CDS             complement(3599..4762)
                     /locus_tag="VFU75_02310"
                     /EC_number="2.6.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine transaminase"
                     /protein_id="HEU5465690.1"
                     /translation="MTATVPGASALLGLYARVGPVFTGGEGVELIAEDGTRYLDFTSG
                     IAVNALGYGHPVIRDAITRALDAGLIHVSNLFRTEPGEALAAELTGRSFADQAFFCNS
                     GAEANEAAFKFARKWSRKTGIVAFSHSFHGRLFASLAATDHPEYQKPFLPLTPGVRIL
                     PLGDRAAAEAGITAEETAAVIIEPVQGEGGVRPVGDEWLAFLRQLCDARGVALIFDEV
                     QCGLGRTGTLFAYEQTGVTPDMLTLAKPLAGGLPMGAVLVTDAIASALATGDHATTFG
                     GGPLVASVAVEVVKTIAEPDFLASVRARGEHLGRGLARIAKASSKIKSVRGRGLMWGV
                     ELSVPAAPVVGAARDRQLLVLSAGPNVLRLLPPLVIEDADLDRGLAVLAEVLA"
     gene            complement(4759..>5608)
                     /gene="argB"
                     /locus_tag="VFU75_02315"
     CDS             complement(4759..>5608)
                     /gene="argB"
                     /locus_tag="VFU75_02315"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HEU5465691.1"
                     /translation="SGGAEHERDAGPPGNGGARVLKVRVVKIGGNELDRPEWVSSCAR
                     ARSGGSGVPAVPTVIVHGGGRAVSYLSRRLSLPGEKREGLRVTTAQVAEVVEMVLAGP
                     VNRLLVTALRSAGIDAIGLSGADGGLLAATQIAGGLGYVGEITRVRTALIESLLLAGL
                     TPVIAPVTPDPAGSAVALNVNADSAAAVVAAALRATELLFVSDVPGVKIGDVPQSKIS
                     SGDIERLIADGTATDGMAAKLRAAQHALSGGAQSVRIGDLRMLTEPSAGTRLVTSASR
                     TSAVLA"
BASE COUNT          950 a         1915 c         1863 g          880 t
ORIGIN      
        1 gcgatcatct cggccgtcac cttcgtttcg ctgcgcacga tgagcgcctc ggccgtcgtc
       61 agagcggcct cgagctcctc acgcgatttc cccgcgaggt tcacgacgtg gatttccgga
      121 gacgacgcca gcagcgcgat ccccgcgtcg ccgagcttgt cggcgatgac gacccgaatg
      181 ctcatcccag gatccgttga atgagggcca gaaggttctc caaggacgcc acggagtggt
      241 cacccatatg tccgatccgg aaggtcgagt ccttcacccc gccgtaaccg gtgccgatga
      301 cccacccctc cttcttcacc gctccgacca ggtccttcga gatcttgccg ttcggcatct
      361 tgatgcacga gacggtccag ctccggcggc cctcgggagg cagataggag agcccgccct
      421 tctggaccca cttctcggtc gtcttgcgca tgtcgtcatg ccgcttccac cgcgcctcga
      481 tgcccccctc cgcgtcgatg cgctcgagct ggacgtccag ggcgaagaag agcgacaggg
      541 cgggtgtgtt ggtcggcgag tacttggcgc tcgccttttc gaaagtgacc aggtcgaagt
      601 agagcccgcg ggacggcagg gtcctggcgc gctcgagcat ccggggagac gcgaccccga
      661 acgccagacc cggcggcagg gcgagggctt tctgcgaccc ggtgaagacg tagtccaggt
      721 cccacgcatc cgtctcgacc ggcgaacccg ccatcgaggt gacggcgtcg accaggaaga
      781 ggacgtccgg aaactcatgg accaccttgg acagggccgc gacgtcctgc agcacgcccg
      841 tggaggtctc cgagtgcgcc aaggtcaccg cgtcgacccc ggggttcttc ttcagggcat
      901 cccgcagctg atcgggctcg acgaagccgc ccagcgggac ctcgaggcgg acgcattcct
      961 tgtcgcaggc cttcaccagg tcggcgaacc gctccgagaa cgagccgttg acgatcgaga
     1021 tcgccttctt ccgtatccct gagcggaccg ccatctccat gaaccccgtg gccgaggtcg
     1081 tgccgaccat gacggtgcgc gaagtccggg ccagctgctg cagcttgggg gcgcagcgct
     1141 tgagcaggtc ctccatggcg gacgagcggt ggccgatcat gggccggtac atcgcggcga
     1201 gaacatccgg atgcacgtcg gtcgggccgg gcaggaaaaa cttcccgaat gcgggcgtgg
     1261 gcaggctcac gcgattcccc ctaacacttc atcggtcttc acgagacgaa cgccttcctc
     1321 tccccattcc ttgaggaggt gctccgcggc gctggcgtcg atgggcttca tcgcgtcggt
     1381 caccgcgaag agtcgggtgt gcagacggtg ggtcaacagg ccttcgatgg cgtagcggtt
     1441 gcacacgtcc agcgccacgc cgtacagcac gatgtcgtcg ggcgacagga ccgtcagcgc
     1501 cggcacgacg ttcgcattgg tgaaaacgtc gaaccagtgc ttgtggaaca gcaagtcgcc
     1561 cttgtgcgcc cgcagcgcat tccccagctc cacgggatcc cgcatttccg gctcgatcac
     1621 catcgggttc ttgagcgcgg tctcggggat tttcttctga gccggcgtgc cgtgcatgca
     1681 atgcgggggg aacgtgtcgc gccagttggg gtgatcggac agctccttgt cgcccgggga
     1741 gtggtcgtcc gccgaggcca ccacccggat gtcgttctgg tgggcgtagt cggtcaggcg
     1801 cttgagattg ggaatgacct tttcggcgtc cgggacatag agcttcccgt ccgctcgcat
     1861 gaaatcgtac tgggtgtcga cgtcccaaaa aatcactcgg gccatgccgc gcctctcagt
     1921 gacgcgtgtc ttcacactct aaagagtgtg ctcggccagt cactgcgctt caagcgcaga
     1981 gccgtgccga gcccaggctt cgagcctggg agaaggaatt ctccctggca ggatgggagt
     2041 gtgtcaacgc ccgcggccga tcagcgccaa tcgaggaaca tccgctcggc gcggtaggcc
     2101 gttgagacgc ggatgcgcga cgtgaacgta tctccttggt ggatcacatg gtcgatggcc
     2161 accgtgacag agtccgaggt gctcgccttg cgcaatttct ggagagtgac ggtgcgggag
     2221 gtgtcgatgc cgagtcccgg ttcggtcgcg ctgaaaaccc atccgagccc gatcgcgcgg
     2281 accgtcgccg tacgaatggc gagaggcaac gccgtgtctg cgacccagtc cccgagatac
     2341 ctcgcgttgc ccgggtcgga ccctgcgatg gtcagcaacc ggtccacccg ccgcagacgg
     2401 gttacaaagc ggtcgagtgg cgctatccgc tcgccggccc gggccgccgc cgccttgccg
     2461 cccgccagcg tggtgtcgcg cgaaagcaca tccatcgcat cgcgctcgat gcgcgcaccg
     2521 accaacaggc gctccagcgc accggactgc tgcatgcccc gcgcgagatt gagcgcggag
     2581 tccatcgcca tcctggcatc cggcaggtca ccgtccccct tccacgtctc cgcggcggcg
     2641 aagagcaccc gggcctgaaa caggcggaag cccgcgggag gggacaccag caccatgctc
     2701 cgggaaaggt gattcttgtc cgttggacgg gagatcgtga ccgaggcgcc actctcttcc
     2761 cagcctttgc gttccttgga tccggccgga aaggtgtctc cgaaagcggg caggttgttg
     2821 ccgctgcggg aggcgcccag caccagcgtc ccgggggatg ttgctccgga gagtgaccag
     2881 ggcggcaggg aggggggaac gagagagtcg gtgatggcaa agccggacag ggtgtcccac
     2941 gcggcgatcc cgtcgtcgct cgtgtacgtc tgctgtgctt cactgccctt gaagagctgg
     3001 tccttggtga gcagcaagcc gatcccgccg aacacgacca aaaggccccc gcaaccgagg
     3061 gccccgaaga cgagtttctt gtggatgtcg gatgccacga cgggaatcta ccccagagcg
     3121 cgcgtcaccg cgatttcgtc acagcaggat tgccgtgggc accgggcgca gtcggcccag
     3181 accttcaggg gaaatctggt cttctcggtg cgggcgaagc cgaggcgcga gaagaagtcg
     3241 ggcttccggg tgagtgcgaa cgtttccgcg agtcccagct cccgggcgtc ttcgagcagg
     3301 ccctcgacga tcgcgcggcc gacgccggca ccctgccacc catcggccac ggcgagaccg
     3361 gcgacttcac ccagcgtctg gctgtagacc ttgagggacc cgcagccgat cacctcaccc
     3421 tcgggcgtgc cagcgatgac gtattccttg atgtgccggc acagatcgta attggaccgt
     3481 gggagcaggt cccccgtcgc gatgaacggc gccatcagct tttcgagtgc gggcacgtcg
     3541 gcgagcgagg ccgggcgaat ctcgagggcg ggatgaagca gcggaagcgg caacgcgctc
     3601 acgcgagtac ctccgccaat acggccagcc cccgatcgag atcggcgtcc tcgatcacca
     3661 gtggcggcaa gaggcgcagc acgttcgggc cggccgacaa aacgagcagt tgccggtcgc
     3721 gcgcggcccc gacgaccggg gcggcaggga cggacagctc cactccccac atcaacccgc
     3781 ggccgcggac gctcttgatc ttcgacgacg ccttcgcgat ccgcgccaag cccctcccca
     3841 ggtgctcccc gcgcgcgcga acggaagcga ggaagtccgg ctcggcgatg gtcttcacga
     3901 cttcgacggc caccgacgcc accaggggac cgccaccgaa cgtggtcgcg tggtcacccg
     3961 tcgcgagcgc actcgcgatt gcatccgtca cgagcaccgc ccccatcggc aagccgccgg
     4021 ccaggggctt tgccagggtc agcatgtcgg gcgtgacgcc cgtctgttcg taggcgaaca
     4081 gcgttcccgt ccgtcccagg ccgcactgga cttcgtcgaa gatcaacgcc accccacgcg
     4141 cgtcgcagag ctggcgcaga aatgccagcc actcgtcgcc gacggggcgg acgccgccct
     4201 ccccctgcac cggctcgatg atcaccgcgg ccgtctcttc ggcggtgatc ccggcctcgg
     4261 cggcggcgcg gtctcccagc gggaggatcc gcacgcccgg ggtcagcggg agaaacggct
     4321 tctggtactc agggtggtcg gtggccgcga gggacgcgaa gagccggccg tggaaggaat
     4381 ggctgaacgc cacgatgccg gtcttcctgc tccacttccg ggcgaacttg aaggccgcct
     4441 cattcgcctc ggcccccgag ttgcagaaga acgcctgatc ggcgaatgac cgccccgtca
     4501 gctcggccgc gagggcctca ccgggttcgg tacggaacag gttcgagacg tgaatcagcc
     4561 cggcgtccag cgcccgcgtg atggcgtcgc ggatgacggg gtggccgtag ccaagcgcgt
     4621 tgaccgcgat cccggacgtg aagtcgaggt accgggtgcc gtcctcggcg atgagctcca
     4681 cgccttcccc gcccgtgaag acgggaccga cacgggcata gagcccgagc agagccgaag
     4741 cccccgggac agttgcggtc acgccaggac ggccgaggtg cgtgaggctg aggtgacgag
     4801 gcgagtgccc gccgaaggct cggtgagcat ccgcagatcg ccgatgcgga ccgactgcgc
     4861 gccacctgaa agcgcgtgct gggcggcacg cagcttggcg gccatgccgt cggtggccgt
     4921 gccgtccgcg atgagccgct cgatgtcgcc gctggagatc ttcgactgcg gtacgtcccc
     4981 gatcttcacg cccggcacgt cggacacgaa caggagttcc gtcgcgcgca acgcggcggc
     5041 cacgaccgcc gcggcgctgt ccgcgttgac gttcaacgca acggcagatc cggcaggatc
     5101 gggcgtgacc ggcgcgatga cgggtgtgag tcccgccaga agcaggctct cgatcagcgc
     5161 ggtccggacc cgcgtgatct cacccacgta ccccaggcca cccgcaatct gtgtagccgc
     5221 cagaagcccg ccgtccgctc ccgacaatcc gatggcgtcg atccccgcgc tccgcaaggc
     5281 ggtcacgagg aggcgattca ccggccccgc caggaccatc tccaccacct cggcgacttg
     5341 cgccgtggtg acgcgcagac cttcacgctt ctcgccgggg agtgacaggc gccgggagag
     5401 ataggaaacc gcgcggccgc cgccatgcac gatgaccgtc gggacggccg ggacgcccga
     5461 accaccggac cgggcccggg cgcacgagga cacccactcg gggcgatcga gctcgttgcc
     5521 gccgatcttg acgacgcgaa ccttcagcac gcgagccccg ccgtttcctg gaggcccagc
     5581 atcacgttca tgttctgcac cgcctgac
//
LOCUS       DASUBP010000051         3548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_519048, whole genome shotgun sequence.
ACCESSION   DASUBP010000051 DASUBP010000000
VERSION     DASUBP010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_519048"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..654)
                     /locus_tag="VFU75_02320"
     CDS             complement(<1..654)
                     /locus_tag="VFU75_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU5465692.1"
                     /translation="MPLPWTLVCSRCNDTRDAGGLPGVCPHDGAPYLVKYPGHPPAEV
                     KSLLKDRRRNMWRYREWLPLADGEQPITLGEGGTPLLPLGRLGKKAGFEHLFVKDEGL
                     NPTGSFKARGLAAAVTRAVLAGASRFVVPTAGNAGVSLAAYATRAGVTARVYAPASTP
                     RTILSQIQRFGADLQLIDGHIGDCGKAARAYAAETGAVDVSTLREPYRIEGKKTLGLE
                     "
     gene            complement(654..2462)
                     /gene="uvrC"
                     /locus_tag="VFU75_02325"
     CDS             complement(654..2462)
                     /gene="uvrC"
                     /locus_tag="VFU75_02325"
                     /inference="COORDINATES: protein motif:HMM:NF001824.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEU5465693.1"
                     /translation="MAIPPELVRTLATLPDRPGVYLWKDAEGGILYVGKAKSLRSRVR
                     SYFGPDHEDVAKHEALVRQIAAVETIVVPTEAEALLLENNLIKAHSPRFNVLLRDDKS
                     YPQIAVTMGEPFPRILVVRRVTIPGARYYGPYTDVGTLRQTLQIIRRLFTVRSCNWKL
                     PEERPDRPCLDYHIERCKAPCVNYQSQEDYRRMIDDVLLFLEGKTVDVRTRLRERMNE
                     ASRVQDYERAAQLRDAIRWLEQIDRPQAVEQVAGGDADAIGFARDGDDACGVILRVRE
                     GRVVAREHRFLEHAEGETEAAVLSAFLVRYYLPLEQRAHRVLLPFGPDDFEALRSLSP
                     DVEWLTPQRGANARVVAVAEQNARHLLDSLMIESFDIEERAADPVYALGRDLGLAAVP
                     RSFICVDISTNQGRDTVGSLVWFESGRPKKSEYRRYKIKGPAQQDDFAAIHEVVTRFV
                     TRRLAEQKALPDLAMIDGGKGQLSAAVDAARGAGAPALPLVSLAKREEHVFFPDRAEP
                     LVLSRRSPSLRLLQRARDEAHRFAVTYSRKRRSARTITSALLEIRGIGPNRRKSLLER
                     FGSLAGVKTATPAEIAALPGFSTALAERILDRLKDR"
     gene            complement(2429..>3548)
                     /locus_tag="VFU75_02330"
     CDS             complement(2429..>3548)
                     /locus_tag="VFU75_02330"
                     /inference="COORDINATES: protein motif:HMM:NF015013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HEU5465694.1"
                     /translation="NTHRRFFLPYAAPAMWNVAGLVAMIGGATWYVNRTLPVEAQLHQ
                     LALALAWGTVAGSLLQIAVQLPTCWRLLRGIPIRVSLIPEGTRDVMRALIPVVVGAGA
                     AQLSGLVDALLSTLTGHGGTSTLGYAQLVQVLPVSLFGASVNAVALPDLSREAVSEGA
                     DQLLRTRVAMGFRRIAYFVLPSAVAFITLGTVLAAAIFQTGRFEARDSHLVGLVLAAY
                     GIGLLGQASVKLFASGFYAMRDTRTPVTIAISCLVLAGLSAFLLLRWLGPAGIALGSS
                     IGWSVSLVFHVRDLNHRIGRILHAADWRYFASALGGAILAGGAGWVVARLAQGLPPIP
                     LAALAFAMFGVMYGAVTVLLRHPDAMRLWQYLRNLSAR"
BASE COUNT          615 a         1175 c         1211 g          547 t
ORIGIN      
        1 ctcgaggccc agggtcttct tgccctcgat gcggtagggt tcgcgaagcg tcgagacgtc
       61 cactgcaccg gtttccgccg catacgcccg ggccgctttc ccgcagtcgc cgatgtgccc
      121 gtcgatgagc tggagatcgg cgccgaagcg ctggatttgc gacagaatgg tccttggcgt
      181 ggaggcgggt gcgtacactc gggcggtcac gcccgcgcgg gtggcgtagg ccgcaaggga
      241 cacgccggca ttgcccgcgg tgggcacgac gaatcgactg gcacccgcga gcactgcccg
      301 ggtgaccgcg gcggccagtc cccgcgcctt gaacgagccg gtcggattca acccctcgtc
      361 ctttacgaac aggtgctcga atcccgcctt ctttccgagg cggccgagcg ggaggagtgg
      421 cgtgccccct tcgccgaggg tgatcggctg ctcgccatcg gcgagcggaa gccattcgcg
      481 gtagcgccac atgttccggc ggcggtcttt caggagagac tttacctccg cggggggatg
      541 gccagggtac ttcacgagat acggcgcgcc atcatgaggg cacacgcccg gcaggccgcc
      601 ggcgtcgcgg gtgtcgttgc aacgggagca cacgagggtc caagggagag gcattatcgg
      661 tccttcaggc ggtcgaggat acgttcggcc agcgcggtcg agaatccggg tagcgcggcg
      721 atttcagccg gggtcgcggt cttcacgccc gccagcgatc cgaagcgctc gagaagcgat
      781 ttgcggcggt ttggcccgat cccccggatc tccagcaggg cagacgtgat ggtgcgggcc
      841 gaacggcgtt tgcggctgta ggtgacggcg aaccggtggg cttcgtcgcg ggcccgctgc
      901 aagaggcgca aggacggact ccggcgggac agaaccagcg gctcagcgcg atccgggaag
      961 aacacgtgct cttcccgctt ggccagactc accaggggaa gtgccggtgc ccccgctcct
     1021 cgtgcggcat ccaccgccgc cgacagctgt ccttttcccc cgtcgatcat cgccaggtcg
     1081 gggagcgctt tctgctcggc gagccggcgc gtcacgaacc gggtgaccac ctcgtggatc
     1141 gccgcaaagt catcctgctg ggcggggccc ttgatcttgt accgccggta ctcgctcttt
     1201 tttgggcgcc ccgactcgaa ccacaccagc gacccgacgg tgtcccgccc ctggttcgtc
     1261 gagatgtcga cgcaaatgaa cgatcgaggg acggcggcca ggcccagatc acggccgagg
     1321 gcgtagaccg gatccgcggc ccgctcttcg atgtcaaacg attcgatcat caatgaatcg
     1381 aggaggtgcc gggcgttctg ctccgcgacg gccaccacgc gggcattcgc cccccgctgg
     1441 ggcgtcagcc actcgacatc cggagacagg gaccgcagcg cctcgaagtc atcgggtccg
     1501 aacggcaaga gcacccggtg ggcccgctgc tcgagcggga ggtagtaccg caccaggaat
     1561 gccgacagca ccgcggcttc tgtttcaccc tccgcatgct cgaggaaccg atgctcccgg
     1621 gcgacgaccc ggccctcccg gacccggaga atgaccccac aggcgtcatc accgtcccgc
     1681 gcgaagccta tggcgtccgc gtcccctccc gccacctgct ccaccgcctg cgggcggtcg
     1741 atctgctcga gccaccggat ggcgtcccga agctgggcgg cccgttcgta atcctgcacc
     1801 cggctcgctt cgttcatgcg ttcgcggagc cgggtccgga cgtcgaccgt cttcccctcg
     1861 aggaacagaa ggacgtcatc gatcatgcgg cgatagtcct cctgcgactg gtagttcacg
     1921 cagggggcct tgcaccgctc gatgtgatag tccaggcagg gccggtccgg acgctcttcc
     1981 ggaagcttcc agttgcagga ccggacggtg aagaggcgcc ggatgatctg cagggtctgc
     2041 cgcagcgttc cgacgtcggt gtaggggccg tagtaccgcg ctcccgggat ggtcacccgt
     2101 cgtacgacca ggatccgcgg gaacggttcg cccatcgtca cggcgatctg gggatagctc
     2161 ttgtcgtccc gcaggagaac gttgaagcgc ggactgtggg ccttgatgag gttgttttcg
     2221 aggagcaatg cctcggcttc ggtcggcacg acgatggttt cgaccgccgc gatctgtcgc
     2281 accagtgctt cgtgcttggc gacgtcctca tggtcgggcc cgaagtacga ccggacgcgc
     2341 gagcggagac tcttggcctt ccccacgtac aaaatccccc cctcggcgtc tttccagaga
     2401 tagacgccgg ggcggtcggg cagagtcgct agcgtgcgga caagttccgg aggtattgcc
     2461 ataggcgcat cgcgtcaggg tgccggagca gtaccgtcac ggctccgtac atcaccccaa
     2521 acatagcgaa ggcgagcgcg gccaatggaa tggggggcag gccctgcgcg aggcgcgcga
     2581 ccacccaccc cgcccctccc gcgaggatcg ccccgccgag tgcggatgcg aagtaccgcc
     2641 aatcggcggc gtgcaggatg cgtccgatcc ggtgattcaa atcgcgcacg tggaacacga
     2701 ggctcaccga ccagccgatg gaggagccga gcgcgatgcc ggcggggccg agccagcgaa
     2761 gcagcaggaa ggcggagagg ccggcaagca cgaggcatga aatggcgatc gtgacgggcg
     2821 tgcgcgtatc gcgcatggcg taaaatcccg atgcaaacag cttgacggac gcctgtccca
     2881 atagcccgat gccgtacgcg gcgagcacga ggcccaccag gtgcgagtcc cgcgcttcaa
     2941 agcgtccggt ctggaagatc gccgccgcga gcaccgtccc cagggtgatg aaggccacgg
     3001 cggacgggag gacgaaatag gcgatgcggc ggaatcccat cgcgacgcga gtgcgcagga
     3061 gctgatccgc cccctcggag acggcttcgc gcgagagatc ggggagtgcg acggcattga
     3121 cggaggcgcc gaagaggctg accggcagga cctgcaccag ttgggcgtac cccagggtgg
     3181 acgtgccgcc atgtccggta agcgtcgaca agagtgcgtc taccagaccg gagagctgtg
     3241 ccgccccggc ccccaccacc acgggaatga gggcccgcat gacgtctcga gtcccctctg
     3301 gaatcaacga gacccggatg ggaatccccc gcagcagccg ccagcaggtg gggagctgaa
     3361 cggcgatttg caacagagag cccgcgaccg ttccccaagc gagcgccagc gccagctgat
     3421 ggagctgcgc ctccacgggc aaggtccggt tcacgtacca cgtcgctccc ccgatcatcg
     3481 cgactagccc tgcgacattc cacatggcgg gtgccgcgta cggcaggaag aaacgccggt
     3541 gagtgttc
//
LOCUS       DASUBP010000052         2367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_927921, whole genome shotgun sequence.
ACCESSION   DASUBP010000052 DASUBP010000000
VERSION     DASUBP010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2367
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927921"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..417)
                     /locus_tag="VFU75_02335"
     CDS             complement(1..417)
                     /locus_tag="VFU75_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEU5465695.1"
                     /translation="MNRKGFTLIELLIVVVIIGILAAIAIPKFANTKQKAYAASMKSD
                     LRNLVTAEEAYFSDSLKYVTTTSCTNPPTAGTAAWCATTGNTLGAITTGTGTQAGWTA
                     SITNINTPTSCAIYIGAVTPTAPATTSNAEGAPVCQ"
     gene            863..>2367
                     /locus_tag="VFU75_02340"
     CDS             863..>2367
                     /locus_tag="VFU75_02340"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5465696.1"
                     /translation="MALAAGHRCSREELMEMLWPSVPAKHARHSLAQAITVIKSKVGG
                     ERIVAARSSISLAAGSVDVDALSVGDGASEVSGTFLDGFDIPGARGFEQWKDEWTSKL
                     LPKIRDSLVHRMDAARRLGDFATVERHGATLLQFDPLSEDGVRGVMEARAWVGDRSGA
                     LKTYESYVTRLAEDLSAEPTVEMVRMATLLREGHHARRPNAVSEPYGRRERRMEAETI
                     IGRTREFSLLFDNWIEVKKGAPRVVVMTGDPGIGKTTLTNAFVSSCQLEGAVVARVQA
                     YDAERELPFAVLSELVRQLVLQRAIGGADPEALAELGRVCADVGNAFPGVPKAPDWSA
                     EVIPIRLADGLLKAVTAAAEDTPVVLVVDDIHAADNSSAAILHMLARRLGHARFMLIL
                     AGRSTELRQASAGALAGDPSIPGLSSIDVGPLTTEASRELVKRIVDSTDHRDAPVDRI
                     VDAGRGNALALELLAREWLDHGPESLLRDLEAIDTTPAPRIGLPPAVRSVF"
BASE COUNT          418 a          708 c          803 g          438 t
ORIGIN      
        1 ttactggcag accggggcgc cttcggcgtt cgaagtcgtc gccggggcgg tcggcgtcac
       61 ggcgccgatg tagatcgcgc aggacgtcgg ggtgttgatg ttcgtgatgc tcgcggtcca
      121 accagcctgc gtgccggtac ccgtggtgat ggcacccagg gtgttgccgg tcgtggcgca
      181 ccacgcggcg gtgccggcgg tgggcggatt ggtgcagctg gtcgtggtga cgtacttgag
      241 cgagtcggag aagtacgcct cttcagcagt cacgaggttg cggaggtccg acttcatgga
      301 cgccgcatac gccttctgct tggtgttggc gaacttcggg atcgcgatcg cggcgagaat
      361 gccgatgatc acgacgacga tcaacagctc gatgagtgtg aaacccttgc gattcatgcg
      421 cttcccccta agagaagtag atgacgctgt gagcgatcct agaccggtgc ccattaacgc
      481 aacggccgtg ccagcctttg cctgagatgc cataaaccgt tgtctctaat gattttacgg
      541 ggccggcgga ccagcgtcag cagctgcccg gaggctcggt aggggccgca aactgcaaag
      601 gggtctcaaa aaacgggaga tttctgggac ttggtgatga acctggatct tgatcggggc
      661 gtggaatgct caagtgattt gcggataagg atttgaaagg tcattgacgg tcgtctcata
      721 tcgtaacaac gtcacaaacc gaccaaatca aatgaccccc gcaccgtttg tcttatccac
      781 cctgggctac ccctccctgc ggtccgccgc gggcgaggca atccgctttc ggacgagaaa
      841 gcatttcgcc gtactcattc gcatggccct ggccgccggt catcgctgct cgcgcgaaga
      901 gctcatggaa atgctgtggc ccagtgtgcc agcaaagcac gcccggcact cactcgctca
      961 ggccattacg gtcatcaaat cgaaagtggg tggagaacgg atcgttgcgg cgcgcagttc
     1021 gatctcgttg gcggccggct ccgtagacgt cgatgccttg tccgtcgggg acggcgcctc
     1081 ggaggtcagc ggcaccttcc ttgacggatt cgatattccg ggcgcgcggg gattcgagca
     1141 gtggaaggac gagtggacgt ccaagctcct ccccaagatc cgcgactcac tggtccaccg
     1201 catggacgcg gcacggcgcc tgggtgactt cgccacggtc gaacgccatg gcgccaccct
     1261 gctccagttc gacccgctgt cggaggacgg cgttcgcggc gtcatggaag cccgcgcctg
     1321 ggtaggggat cggagcggtg ccctcaagac ctatgagagc tatgtcactc ggctcgccga
     1381 ggatctgagc gcggaaccga cggtggaaat ggttcggatg gccacgctgt tgcgggaggg
     1441 ccatcatgcc cggcgtccga acgccgtctc ggaaccctac ggccggcgtg agcggcgcat
     1501 ggaggcggag accatcatcg gacggacgcg cgagttctcc ctgctgttcg acaactggat
     1561 cgaggtgaag aagggagcgc cacgggtggt ggtcatgacc ggtgacccag gaatcgggaa
     1621 gaccacactg acgaacgcct tcgtgtcgag ttgccagctg gagggcgccg tcgtggctcg
     1681 cgtccaggcg tacgatgccg agcgagagtt gccgtttgcc gtcctgtcgg agctcgtgag
     1741 acagctggtg ctgcagcgag ccatcggtgg tgccgacccg gaagcgttgg cggagttggg
     1801 ccgggtctgt gccgacgtgg ggaatgcctt cccaggtgtg ccgaaggcgc ccgactggtc
     1861 agcggaagtg attcccatcc gtctggccga tggcctgctg aaggcggtca ctgccgccgc
     1921 agaggacacc ccggtggtgc tggtcgtcga cgacatccat gcggccgaca attccagcgc
     1981 cgcgatcctg cacatgctgg ccaggaggct gggacacgcg cgattcatgc tgatcctcgc
     2041 cggtcgttcc accgagcttc ggcaagcaag tgcgggcgca ttggctgggg atccgagtat
     2101 ccccgggctg tcgagtatcg acgtcggtcc cctcacgacc gaggcgtcgc gagaactcgt
     2161 gaagcggatc gtggattcga ccgatcaccg ggatgcgccc gtggaccgga tcgtggacgc
     2221 ggggcgcgga aacgccctgg cgctcgagct gttggcccgg gaatggcttg accacgggcc
     2281 cgagtctctc ctgcgagatc tcgaagccat cgacacaacg cccgctccac ggatcggcct
     2341 ccctcccgca gtgcgcagcg tcttcga
//
LOCUS       DASUBP010000053        11112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1159445, whole genome shotgun sequence.
ACCESSION   DASUBP010000053 DASUBP010000000
VERSION     DASUBP010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 11112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11112
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1159445"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3590)
                     /locus_tag="VFU75_02345"
     CDS             complement(<1..3590)
                     /locus_tag="VFU75_02345"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004559 - alpha-mannosidase activity
                     [Evidence IEA];
                     GO_process: GO:0006013 - mannose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 38 C-terminal
                     domain-containing protein"
                     /protein_id="HEU5465697.1"
                     /translation="MMNALTAALLLLIQQPHVTPQIPIAIRPWLLTPPVSTDTGATRL
                     TRDYLGGEAAAFPDSSGTWRPVTIPASGIDDLGKEFGGAKERTVVYAFTYVYAPKDES
                     RTLVLASDDDILAWLNGQRIHYHDIPRGVDHDRDTVEVRFAKGWNALLLKVVNRGGDF
                     GFGAWIVGDSMHAANRRPGDAQPGNLPASTVAAGPLRLSGPVTWSHDTLLGTGSTTIT
                     TWGRNPPSSAVVSLSAGGQRLSSDSVDSLVPGTPKSLHRAFDLATLSRVALDSTAMLT
                     ARMGTTDDARTAPLDPAAVLSLLDSRITFGNWNDHGTTLETTVTVPPAFVGLSVDLLA
                     AEFGPTARYSINGQSKMLAADGTLSLCSQCATGDTLHITIVRDSTRAWWDHPRARVRD
                     RSYSDVALDLTLLPALGDSSGSVPWPDARAWLSAMITPDKSVYRLLEKQSAQSLASDS
                     ALFAKDTIHLVGNSHIDAAWLWRYTETMGVVEATWRTALKLEQKFPSMTFAASAAQYY
                     DWLAQRSPGLLDSIRAAEHAGRWEAVGGWWLEADQNMPGGESLVRQGLYGQRYFQKLF
                     GRRSKVAWTPDSFGYPWQMPQIWHGLGMDFFVTQKIRWNDSTTFPYDAFRWKGRDGTT
                     IFAYNPYGYDHDLDGATLAQQMRQDNARTDTAHHMLVLYGVGDHGGGPTAEMLDRRGD
                     LGRLPAFPVLKDDRPTTALSSIRGARPMDKWPLWNDELYLEYHRATYTTQSWMKRRNR
                     QSETLLGTAEFLATVDPAPYPKDSLEHAWRQTLFNEFHDLLPGSGIHPIYTDAMATYD
                     SVAAAGTAIRDRSVHDLSARLDTRGRGVPVTVFNPSSWARTGFVSVAVTDGLRSLSGA
                     LVAADERGRTQPAVIRGDSLRFRATDVPSLGATVFWIRPGRIPDDRTFAGSRTHLENE
                     YLSVDVDPKTGQIVHLYDRKAHHEAVSDSGRANVLQLLVDEPAQWDAWNTGFTGKLWT
                     IDSVKALRAGGDEVERWIEVEKPWNGTHITQRIILRRGEPYLEIDNQIDWHERHKFLK
                     AAFDWNVTADSATYEIGYGAIGQPTNPSTQAERAKYEHPGHRWGDLSDSVFGVSVLND
                     SKYGWDTRGHRMRVSLLRSPLWPDSLADRGPQRFRYAIYPHSGGWQSGLTEREGQAFN
                     IPMIAVATSEHAGVAGKAWSVAATSEPDVYITAVKRAEDGPGLVLRVVEW"
     gene            complement(3590..4138)
                     /locus_tag="VFU75_02350"
     CDS             complement(3590..4138)
                     /locus_tag="VFU75_02350"
                     /inference="COORDINATES: protein motif:HMM:NF013492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU5465698.1"
                     /translation="MTVRRIHQLGDPILRARCQKVDKPSSAAVRLVADDLRDTLSEAR
                     KKFKMGRALAAPQIGAPMRLVFMQVGRQRWNLVNPEITDVGPDDFLVWDDCFSFPDLY
                     VRVTRAYQVTLSYQDLQGKPHTMSLEGPMAELVQHEIDHLDGILAVDRASGLDPFAYR
                     EEWEKIHEPADRYGPPSPREML"
     gene            4196..4813
                     /locus_tag="VFU75_02355"
     CDS             4196..4813
                     /locus_tag="VFU75_02355"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU5465699.1"
                     /translation="MSLAGIVLAGGRSSRMGSPKALLDFRGEPFVLRILEAFEALDLK
                     TRVVVVGPGVEAQEIRSSLSRHECLVVENPDVDGGPIASLRAGLKAIQSVAPTGAVVW
                     PVDLPHVRVDTVERLLDVFRRNSPFAAVPRFGNRRGHPVIWSSAAFAELEGSEAARRD
                     GARVVLRAHQADIKEVPVDDPAVIDDLNTPEDYERLVREINRDAY"
     gene            4972..6405
                     /gene="sufB"
                     /locus_tag="VFU75_02360"
     CDS             4972..6405
                     /gene="sufB"
                     /locus_tag="VFU75_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEU5465700.1"
                     /translation="MTSIEAQVLQPYKYGFVTDIESEDVPLGLNEDIIRLISRKKEEP
                     AFLLEWRLKAYRHWLTLTEPTWANIHHAPIDYQAIRYYSAPKQQKKLDSLDQVDPKLI
                     ETFDKLGIPLAEQKRLTGVAVDAVFDSVSVATTFKAELAKYGIIFCAFSDAVKNHPEL
                     VEKWLGDVVPASDNYFAALNSAVFSDGSFCYIPKGVRSPLELSTYFRINASSTGQFER
                     TLIIADEGSYVSYLEGCSAPVRDENQLHAAVVELIALDDATIKYSTIQNWYPGDENGK
                     GGIYNFVTKRGACRGKNSKISWTQVETGSAITWKYPGCILQGDNSIGEFYSVAVTNNH
                     QQADTGTKMIHMGKNTRSTIVSKGISAGKGQNTYRGLVKILKGAEDARNYSQCDSMLI
                     GDQCGAHTFPYIDVRNSSAIMEHEASTSKIGEDQIFYCKQRGLSAEDAVSLIVNGFCK
                     EVFKELPMEFAVEAQNLLGISLEGSVG"
     gene            6402..7178
                     /gene="sufC"
                     /locus_tag="VFU75_02365"
     CDS             6402..7178
                     /gene="sufC"
                     /locus_tag="VFU75_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEU5465701.1"
                     /translation="MKHGSREPGAVLLEIKGLHATVATDAKKEILKGLNLTVRAGEVH
                     AIMGPNGSGKSTLAAVLAGHPAYTVTGGSVTYKGNDLLDMDPEERAREGVFLAFQYPV
                     AIRGITNSYFLRSALNAVRKHRGQEELDPLDFLSVLEKKLKQIGWDDSFLNRAVNDGF
                     SGGEKKRNEILQMAVLEPTLAILDETDSGLDIDALKTVAEAVNTLRRPDSATVVVTHY
                     QRLLNYIVPDRVHVLAGGRIVKSGGKDLALELEEKGYVGV"
     gene            7175..8323
                     /gene="sufD"
                     /locus_tag="VFU75_02370"
     CDS             7175..8323
                     /gene="sufD"
                     /locus_tag="VFU75_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEU5465702.1"
                     /translation="MNWLTELRERGAARFAEVGFPTTKQEAWRFTDVRPIAETSYGKA
                     TSRWSVEANGAKDLVTSDLSKAGDIAQAHLGRYADVAANPFTALATAFLDAVQVISVP
                     AKTEVTAPIRLSHRFEGNGAVMATPRVLIVLGDQARATIIETHEDSGIDSSLSNGVTE
                     IVLGDGAILDYIRVQREGTKTSHIATTQSHQGRDSVLRSTSIALGAGLTRHDLGAVLA
                     GTGGSLTLNGLSVISGRQHVDYHTTIDHAQPHCESHELFNGVFDQHARGVFNGRIIVR
                     PGAQRTDSKQTNNNLLLSGEARADSQPQLEIYADDVKCTHGATLGPLDPTALFYLESR
                     GLSPATAKSLLTYGFAADVLTRIAVAPVRDDLDRVIRGRLGVESLAAA"
     gene            8320..9552
                     /locus_tag="VFU75_02375"
     CDS             8320..9552
                     /locus_tag="VFU75_02375"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020359689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEU5465703.1"
                     /translation="MSTTKAWDVTRVRRDFPILATQAHGKPLVYLDNGATSQKPQSVI
                     DAVSHYYESGNANVHRGVYALSESATAAYEGTRAKVARFLNAREDREIIYVRGTTEAI
                     NLVASSFGRLKVKAGDEILVSAMEHHSNIVPWQMLAEQTGAGLKVIPMNERGELKLDE
                     YQKLLTPKVRIVAVTHISNSLGTINPIKEMVKLAHAKGVPVLVDGAQGAPHVVVDVRD
                     LDCDFYTVSGHKMFGPTGVGVLYGKAAHLEAMPPYQGGGDMIRSVTFAKTTYAPIPAK
                     FEAGTPDIGGVVGLGAAVDYLGALDRTAAAAYEQDLLEHATKQLGTVPGLRIVGTAAH
                     KASVISFVLDGIHAHDVGTIVDQAGVAIRTGHHCTQPVMEFFNVPATARASFVFYNTR
                     DEVDILVEALKGVRKVFG"
     gene            9552..10004
                     /locus_tag="VFU75_02380"
     CDS             9552..10004
                     /locus_tag="VFU75_02380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEU5465704.1"
                     /translation="MADLSELYQQTIVEHNRSPRNFKTLEHPTHHAEGNNPLCGDQIS
                     LQLTVDGGVIKEVGFQGSGCAISRASASLMTSAVMGKSPADAERLFRAFHDMVAGGDA
                     APADPATLGKLAAFSGVKQFPARVKCANLAWHTLHAALQADQTPISTE"
     gene            10083..11039
                     /locus_tag="VFU75_02385"
     CDS             10083..11039
                     /locus_tag="VFU75_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5465705.1"
                     /translation="MAAPAVQLDRITKRFAGHVAVDSLSLQVPSGSIYGLLGPNGAGK
                     STSIRMLMHIHIPDEGTVRLFGEDGKTGREQSSRIGYLPEERGLYPKMRVLDVLIFLA
                     EMKGVDRRRARTLALQWLERLGLADWKMRKVNELSKGMQQKVQVISTLLHEPDLLVLD
                     EPFSGLDPVNAQVLKDTVLELRNKGKTILLSTHIMEQAEQLCDGVCIIARGKKLVDGP
                     LAEVKRTHGGKHVHVTFDGAAGDAERLFSDPKLVKKKESTGQYAELELAQGADAQDIL
                     KGLVTSGARLAKFELAEPSLNKIFVDLVGPEAAKAAAQPEVK"
BASE COUNT         2026 a         3511 c         3673 g         1902 t
ORIGIN      
        1 tgccattcga ccacccgcag caccagcccc ggtccatctt ccgcgcgctt cacggcggtg
       61 atgtagacgt ccggttcgga tgtcgccgcc acggaccacg ccttgcccgc cacgccggca
      121 tgctccgaag tcgcgaccgc gatcatgggg atgttgaatg cctggccctc acgttcagtg
      181 aggccggact gccacccgcc cgagtgcgga tagatcgcgt accggaagcg ttgcggtccg
      241 cgatcggcga gtgagtcggg ccagaggggt gaccggagca aggacacccg catccggtgc
      301 ccgcgagtgt cccagccgta cttcgaatcg ttcagcacac tgacaccgaa taccgagtcg
      361 gagagatcgc cccaccggtg tccggggtgt tcgtatttcg cgcgctccgc ctgcgtcgag
      421 gggttcgtgg gttgaccgat ggcgccgtac ccgatctcgt aggtggcgct gtcggccgtc
      481 acattccagt cgaaggcggc cttgaggaac ttgtgccgct cgtgccagtc gatctggttg
      541 tcgatttcga ggtacggctc accccggcgg aggatgatcc gctgagtgat gtgcgtgccg
      601 ttccacggct tctcgacttc gatccaccgt tcgacttcat cgcctcccgc ccggagcgcc
      661 ttcaccgaat cgatcgtcca gagcttgccc gtgaaccccg tgttccaggc gtcccactgg
      721 gccggctcgt cgaccaggag ctggagcacg ttcgcgcgcc ccgaatccga cacggcctca
      781 tggtgcgcct tgcggtcgta gagatggacg atctgtcccg tcttcggatc gacgtccact
      841 gagaggtact cgttctcgag atgcgtccgg gagccggcga aggtccggtc gtcgggaatc
      901 ctccccggcc ggatccagaa caccgtggcg cccagagacg gcacgtcggt cgcccggaaa
      961 cgcagggagt ctccacgaat caccgccggc tgcgttcttc cacgctcatc cgctgcgacc
     1021 agcgctccag acaggctccg cagtccgtcc gtcacggcca ccgatacgaa cccggtccgg
     1081 gcccacgacg agggattgaa caccgtgacg gggacccctc ggccgcgcgt atcgaggcgg
     1141 gcggagagat catgaacgga gcgatcccgg atggcggtcc ccgcggcagc gacggagtcg
     1201 taggtcgcca tggcgtccgt gtagatggga tggatgccgg acccggggag caggtcatgg
     1261 aactcgttga agagcgtctg ccgccaggcg tgctcgagag agtccttcgg gtagggcgcc
     1321 gggtcaaccg tcgcgaggaa ctccgcggtg ccgagcaggg tctcgctctg ccgattgcgg
     1381 cgcttcatcc agctttgggt cgtgtacgtc gcgcggtgat actcgagata cagctcgtca
     1441 ttccagaggg gccatttgtc catcggccgc gcgccccgaa tgctcgacaa cgccgtcgtc
     1501 ggcctgtcgt ccttcaggac cggaaacgcc ggaagccttc cgagatcgcc ccgccggtcg
     1561 agcatctccg ccgtagggcc gccgccatga tcgcccacgc cgtagagcac gagcatgtgg
     1621 tgcgccgtgt cggtccgggc attgtcctgg cgcatctgct gggcgagcgt cgcgccatcg
     1681 aggtcgtggt cgtagccgta ggggttgtag gcgaagatcg tcgtcccgtc ccggcccttc
     1741 catcggaacg cgtcgtacgg aaacgtggtc gagtcattcc agcggatctt ctgcgtgacg
     1801 aagaagtcca tgccgagccc gtgccagatc tgcggcatct gccagggata gccgaaggaa
     1861 tcaggggtcc aggccacctt cgagcggcgc ccgaagagtt tctggaagta gcgctggccg
     1921 tagagcccct gcctgaccag tgattcgccg ccgggcatgt tctgatcggc ctcgagccac
     1981 cagccaccga ctgcttccca acggccggca tgttccgcgg cgcggatcga gtcgagcaat
     2041 ccgggcgatc gctgtgccag ccagtcgtag tactgtgcgg cgctcgcggc gaaggtcatc
     2101 gacgggaact tctgctcgag cttgagcgcc gtccgccacg tcgcctcgac cacgcccatc
     2161 gtctcggtgt agcgccagag ccaggcggcg tcgatgtgcg agttgccgac gaggtggatg
     2221 gtgtccttcg cgaagagcgc cgagtccgag gcgagactct gcgcactctg tttttcaagg
     2281 agacgataga ccgacttgtc gggcgtgatc atggctgaga gccaggcgcg cgcatcgggc
     2341 cacggaaccg aaccggacga gtcgccgagg gcgggcagga gcgtgaggtc gagggcgacg
     2401 tccgaatagg accggtcgcg cacacgcgcg cgcgggtgat cccaccaggc cctggtcgag
     2461 tcgcggacga tggtgatatg cagcgtgtcc ccggtcgcgc attgcgagca cagagacaat
     2521 gtgccgtccg ccgcgagcat cttggattga ccgttgatcg agtaccgagc agtggggccg
     2581 aactcggcag ccaggaggtc gacggacaac cccacgaagg ccggcggcac ggtgacggtg
     2641 gtctcgagcg tcgtcccatg gtcgttccag ttgccgaacg tgatccggga atcgaggagg
     2701 ctcagaaccg cggccggatc gagcggtgcc gtgcgggcat cgtcggtggt ccccatgcgc
     2761 gccgtcaaca tcgcggtcga atcgagagcg acgcgactca aggtcgcgag atcgaaggca
     2821 cggtgaaggg atttcggcgt cccggggacc aggctgtcaa cggagtccga gctgaggcgt
     2881 tgaccgccgg ccgacagcga aacgacggcc gacgacgggg gattcctgcc ccacgtagtg
     2941 atcgtcgtgc ttccggtgcc cagcagcgtg tcatgcgacc aggtcacggg tccggaaagg
     3001 cgcagcggtc ccgcggcgac agttgaagcc ggcagattcc ccggctgcgc gtcgcccgga
     3061 cggcgattcg cggcgtgcat cgagtcgccg acgatccacg caccgaaccc gaagtcaccg
     3121 ccgcgattga cgactttcaa gaggagtgcg ttccacccct tggcgaaacg cacctcgacg
     3181 gtgtcgcggt cgtgatcgac gccgcgggga atgtcgtgat agtgaatccg ctggccattc
     3241 aaccaggcga gaatgtcgtc gtcactcgcg agcaccagcg tccggctctc atccttcggc
     3301 gcgtagacgt acgtgaacgc gtagaccacc gtgcgctcct tggcgccgcc gaactccttg
     3361 cccaggtcgt cgatgccgct cgccggaatg gtgacggggc gccacgtgcc cgaggaatcg
     3421 ggaaacgccg cggcctcgcc gcccaggtaa tcgcgggtga gtctcgtcgc tcccgtgtcg
     3481 gtggataccg ggggagtgag gagccagggg cgaatcgcga tcggaatctg aggagtgacg
     3541 tgtggctgct gaatcagaag aaggagggcg gcggtcagcg cattcatcat cagagcatct
     3601 ctcgaggtga gggcggcccg taacggtcag cgggttcgtg aattttctcc cattcctcgc
     3661 ggtacgcgaa agggtcgagt cccgacgcgc ggtccaccgc gaggatgcca tccagatgat
     3721 caatttcatg ctgtaccagc tcagccatgg gaccttccaa cgacatggtg tggggtttcc
     3781 cctggaggtc ctgatagctc agcgtcacct ggtacgcccg cgtgacccgg acgtaaaggt
     3841 cggggaagga aaagcagtcg tcccagacga gaaaatcgtc tggacccacg tccgtgattt
     3901 ccggattcac caggttccac cgctggcggc ccacctgcat gaagaccagg cgcatggggg
     3961 cgccgatctg cggtgccgcc agggcccgtc ccatcttgaa cttcttccgg gcctcggaga
     4021 gggtatcccg caggtcgtcc gccaccaggc gcaccgcggc cgacgaaggt ttgtcgacct
     4081 tctggcagcg ggcccggagg atcgggtcgc cgagctggtg aatccgccga acagtcatgg
     4141 gggcccccaa tgtggcgatt tgccgggcca tcgcaatacg ggctaacgta agtccatgtc
     4201 actggcgggt atcgtcctcg cgggcggaag atcttcccgg atgggctctc ccaaggccct
     4261 cctcgatttc cggggtgagc cttttgtgct gcgcatcctc gaggcattcg aagcactcga
     4321 cctcaagacg cgcgtggtgg tagtgggccc tggtgtcgag gcgcaggaga tcagaagctc
     4381 gctctcccgc cacgagtgcc tggtggtcga gaaccccgat gtggacggtg gtccgatcgc
     4441 gtcgctacgc gccggcctga aagccatcca atcagtcgcg cccacggggg ccgtggtttg
     4501 gcccgtggat ctgccgcatg tccgggtcga tacggtcgag cggctgctgg atgtctttcg
     4561 ccgcaacagt cccttcgcgg cggtccctag gttcggcaat agacggggtc atccagtcat
     4621 ctggtcgagc gcggcattcg cggagctcga agggagcgag gctgccaggc gcgacggcgc
     4681 gcgggtggtc ctgcgcgcgc atcaggcgga catcaaagag gtgccggtgg acgacccggc
     4741 tgtgatcgac gacttgaaca cgccagagga ctacgagcgg ctggtgaggg agataaatcg
     4801 agacgcgtac tgagaggggt caggggtcgg gggccagggg tcaggggaac tcactgaacg
     4861 atgagcactt actggcccct gacccctgat ccctgagccc ttgtcgaatc ccgactattc
     4921 gactaggatt aggtatcatc gacgacgcaa cttaaggcta ccaaatcact tatgacgtca
     4981 atcgaagccc aggtcctcca accctacaag tacgggttcg tcaccgacat cgagtcggaa
     5041 gacgttcccc tcgggctgaa tgaggacatc atccggctca tttcccggaa aaaagaagag
     5101 cctgccttcc tgctggagtg gcggctcaag gcctaccggc actggctgac cctgaccgag
     5161 ccgacgtggg ccaacatcca ccacgccccg atcgactacc aggccatccg gtactactcg
     5221 gccccgaagc agcagaagaa gctcgactcc ctggaccagg tcgatcccaa gctcatcgag
     5281 acgttcgaca agctggggat cccgctcgcc gagcagaagc ggctcacggg agtggccgtg
     5341 gatgcggtgt tcgactcggt ctcggtggcg accaccttca aggcagagct cgccaagtac
     5401 gggatcatct tctgcgcgtt ttcggatgcc gtgaagaacc accccgagct ggtcgagaag
     5461 tggctgggtg acgtcgtgcc ggcgagcgac aactacttcg ccgcgctcaa ctccgcggtc
     5521 ttctcggacg gctccttctg ctacatcccg aagggtgtgc gctccccgct cgagctctcg
     5581 acctacttcc gcatcaacgc gtcgtcgacc ggccagttcg agcgcacgct catcatcgcc
     5641 gacgaaggca gctacgtctc ctacctcgaa ggctgctcgg cgccggtgcg ggacgagaat
     5701 cagctgcacg ccgcggtggt cgagctgatc gcgctcgacg acgcgaccat caagtactcg
     5761 accatccaga actggtatcc cggggacgag aacggcaaag gcgggatcta caacttcgtc
     5821 accaagcggg gtgcctgccg cgggaagaac tccaagatct cgtggacgca ggtggagacc
     5881 ggctccgcga tcacctggaa atatccgggc tgcattctcc agggtgacaa ctcgatcggc
     5941 gagttctact cggtggcggt gaccaataac caccagcagg cggacaccgg cacgaagatg
     6001 atccacatgg gcaagaacac ccgctcgacc atcgtttcga agggaatctc ggcgggcaaa
     6061 ggacagaaca cctaccgcgg gctcgtgaag atcctgaagg gcgccgagga cgcgcggaac
     6121 tattcgcagt gcgattccat gctgatcggg gaccagtgcg gcgcgcacac gttcccctac
     6181 atcgacgtcc ggaactcgtc ggcgatcatg gagcacgaag cgtcgacgtc gaagatcggc
     6241 gaagaccaga tcttctattg caagcagcgg ggactctcgg cggaggacgc cgtgtccctc
     6301 atcgtcaacg gattctgcaa ggaagtgttc aaggagctgc cgatggagtt cgccgtcgaa
     6361 gcccagaacc tcctcggcat ctcacttgaa gggagcgtcg ggtgaaacac gggagccggg
     6421 agccgggagc cgtcctgctc gagatcaagg gactgcatgc gaccgtcgcg acggatgcga
     6481 agaaggagat cctcaagggt ctcaatctga ccgtcagggc gggcgaggtg cacgccatca
     6541 tggggcccaa cggctccggc aagagcacgc tcgcggcggt cctcgccggt catccggcgt
     6601 acaccgtgac cggtggctcc gtgacctaca agggcaacga tctgctcgac atggatcccg
     6661 aggagcgcgc gcgcgaaggc gtgttcctgg cgttccaata tccggtcgcg atccgcggca
     6721 tcacgaactc ctacttcctg cgctcggcgc tcaatgcggt tcggaagcat cggggacagg
     6781 aagagctcga ccctctcgat ttcctgtcgg tgctggagaa gaagctcaag cagatcggct
     6841 gggacgattc gttcctgaac cgcgcggtga acgatgggtt ctcggggggc gaaaagaagc
     6901 ggaatgagat cctccagatg gccgtcctcg agccgactct tgcgatcctc gacgagaccg
     6961 actcggggct cgacatcgac gcgctgaaga ccgtcgccga ggcggtgaac actctgcgac
     7021 ggcccgactc tgcgaccgtg gtcgtgacgc actatcagcg cctgctcaac tacatcgttc
     7081 ccgatcgggt gcacgtgctg gccggtggac ggatcgtgaa gtcgggtggc aaggacttgg
     7141 cactggagct cgaagagaag gggtacgtgg gcgtgtgaac tggctcactg agttgcggga
     7201 gcgtggggcg gcgcgctttg cggaagtggg cttccccacc acgaagcagg aggcgtggcg
     7261 tttcaccgat gtcaggccca tcgccgagac ctcgtacggc aaggcgacgt cccggtggag
     7321 tgtggaggcc aatggcgcca aggacctggt aaccagcgac ctctcgaagg ccggggacat
     7381 cgcccaggcg cacctgggcc gttacgccga cgtcgccgcg aatcccttta ccgccctggc
     7441 gacggcgttc ctggatgccg ttcaggtgat ttcggtaccc gccaagactg aagtgaccgc
     7501 accgattcgg ctgtcgcacc ggttcgaggg aaatggcgcc gtcatggcca ccccacgggt
     7561 cctgatcgtc ctgggcgacc aggcccgcgc gacgatcatc gagacccacg aggattccgg
     7621 gatcgattcg tccttgtcga acggcgtgac cgagatcgtg ctgggtgacg gcgcgatact
     7681 ggactacatc cgggtacagc gcgaagggac gaagacgagc cacatcgcta ccacccagtc
     7741 ccaccagggc cgggacagcg tgttgcggtc gacctcgatc gcgctgggcg ccggactgac
     7801 ccggcacgat ctcggagccg tgctggcggg aaccggtggg tcgttgacgc tcaatgggct
     7861 gtcggtgatc tctggccggc agcacgtcga ctaccacacc accatcgatc acgcccagcc
     7921 gcactgcgag agccacgagc tcttcaatgg agtgttcgac cagcacgcgc gcggcgtctt
     7981 caacggcagg atcatcgtca ggcccggtgc gcagcggacc gactcgaagc agacgaacaa
     8041 caatctcctg ctctcgggtg aggcgcgcgc cgattcgcag ccccagctcg agatctatgc
     8101 cgacgacgtg aagtgcaccc acggcgccac cctcggcccc ctggatccga ctgccctctt
     8161 ctatctcgag agccgcggcc tgtcgcccgc caccgccaag agcctcctga cctacgggtt
     8221 cgcggcggac gtcctgaccc gcatcgccgt cgcgccggtt cgcgatgacc tggaccgcgt
     8281 gattcgcggc cggctcggcg tggagtcgct ggcggccgca tgagcaccac caaggcctgg
     8341 gacgtcaccc gggtccgccg cgacttcccg atcctcgcca cccaggctca tggcaagccg
     8401 ctcgtgtacc tcgacaacgg tgccacgagc cagaagccgc agtcggtcat cgacgcggtc
     8461 agccactact acgaatcagg gaatgccaac gtccaccggg gcgtgtacgc gctgagcgag
     8521 tcggcgacgg cggcctatga ggggacgcgc gccaaggtcg cccggttcct gaatgcccgc
     8581 gaagaccgcg agatcatcta cgtccgcggt accaccgagg cgatcaatct cgtcgcgtcg
     8641 agcttcggcc ggctgaaggt gaaggccggt gacgagattc tcgtttccgc catggagcat
     8701 cattcgaaca tcgttccctg gcagatgctg gcggagcaga cgggtgcggg gttgaaggtg
     8761 atcccgatga atgagcgggg cgagctcaag ctggatgagt atcagaagct cctgacgccg
     8821 aaggtaagga tcgtcgcggt cacgcacatc tccaactccc tgggaaccat caacccgatc
     8881 aaggagatgg tgaagctcgc gcacgcgaag ggcgttcccg tgctggtgga tggggcgcaa
     8941 ggcgcgccgc acgtcgtggt ggatgtgcgg gatctcgact gcgacttcta cacggtgtcc
     9001 gggcacaaga tgttcgggcc gacgggcgtc ggcgtgctgt acggcaaggc cgctcacctc
     9061 gaggcgatgc ctccctatca gggtggcggt gacatgatcc ggtcggtgac gttcgccaag
     9121 accacgtatg cccccattcc ggcgaagttc gaggcgggga ctcccgacat cggtggcgtg
     9181 gtcgggctgg gggctgctgt cgactacctg ggtgcgttgg atcggaccgc cgccgccgcg
     9241 tacgagcagg acctgctcga gcacgccacc aaacagctcg ggacggttcc cggactgcgg
     9301 atcgtcggta ccgcggctca caaggccagc gtcatctcat tcgtgctgga tggtattcac
     9361 gctcacgacg tcgggacgat cgtggatcag gcgggagtcg ccatcaggac cgggcatcac
     9421 tgtactcagc cggtgatgga gtttttcaac gtcccagcaa cggctcgggc gtccttcgtc
     9481 ttctacaata cgcgcgacga ggtggacatc ctggtcgagg cgctcaaggg cgtgcgaaag
     9541 gtgttcggtt gatggccgac ctgtcggagc tctaccagca gaccatcgtc gagcacaacc
     9601 ggagcccccg gaacttcaag acgctcgagc atccgactca tcacgccgaa ggcaacaacc
     9661 cgctctgtgg ggaccagatc agccttcagc tcacggtgga tggtggcgtg atcaaggaag
     9721 tcgggttcca gggctcaggg tgcgccatct cccgtgcctc ggcctccctg atgacctcgg
     9781 cggtgatggg caagagcccc gccgatgccg agcggctctt tcgggctttc catgatatgg
     9841 tggcaggagg ggacgcggct ccggccgatc ccgccacgct ggggaagctt gcagccttct
     9901 ccggcgtgaa gcagttccct gcccgggtga aatgcgcgaa tctcgcatgg cacaccctcc
     9961 acgccgccct gcaggctgac caaaccccca tcagcaccga gtaacttctc ctcgcttttc
    10021 gccgtagaat tgttcgctgt ctcggtcgtc ccctcacacc cgtcacctgg ctcctgaaac
    10081 ccatggctgc tcccgccgtc cagctcgacc ggatcaccaa acgcttcgcc ggccatgtcg
    10141 ccgtcgactc cctttccttg caggtgccgt ccggctcgat ctacggtctc ctcggcccca
    10201 atggcgccgg gaagagcacg tcgatccgga tgctgatgca catccacatc ccggacgagg
    10261 gaacggtgcg tctcttcggt gaggacggga agacggggcg cgaacagtcc tcccgtatcg
    10321 ggtacctgcc cgaggaacgg ggtctctatc ccaagatgcg ggtactcgac gtcctgatct
    10381 ttctggccga gatgaaggga gtggaccggc ggagggcacg gaccctggcg ttgcagtggc
    10441 tcgagcggct ggggctggcg gactggaaga tgcgaaaggt caacgagctc tcgaagggca
    10501 tgcagcagaa ggtgcaggtc atctccacgc tgctgcacga gcccgatctc ctggtcctcg
    10561 atgaaccctt ctcggggctc gatcccgtca acgcgcaggt tctgaaggac accgtgttgg
    10621 agttgaggaa caaggggaag acgatcctcc tgtccacgca catcatggag caggcggagc
    10681 agctctgtga cggggtctgc atcatcgcgc gcggcaagaa gctcgtggac gggcccctcg
    10741 ccgaagtgaa gcggacgcat ggcgggaagc acgtccatgt gacgttcgat ggcgccgcgg
    10801 gagacgccga gcggctgttc tccgatccga agctggtcaa gaagaaggag agcacggggc
    10861 aatacgccga gctggagctc gcccagggag cggacgcgca ggacatcctg aaggggttgg
    10921 tcacctcagg ggcccggctc gccaagttcg agctggccga gccttcactg aacaagattt
    10981 tcgtggatct cgtgggaccc gaagccgcga aagcggcggc tcagccggag gtcaaatgaa
    11041 caagattttc gcgatcgtcc ggcgggagtt cgtcgagcgg gtgcgccaac gctggttctg
    11101 ggtgatggcc ct
//
LOCUS       DASUBP010000054         6342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_791707, whole genome shotgun sequence.
ACCESSION   DASUBP010000054 DASUBP010000000
VERSION     DASUBP010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6342
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_791707"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..662)
                     /locus_tag="VFU75_02390"
     CDS             complement(144..662)
                     /locus_tag="VFU75_02390"
                     /inference="COORDINATES: protein motif:HMM:NF016840.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5465706.1"
                     /translation="MIEIERPLAGEFNPYYQQYIDQVPKGNLLDILARQGMEFGALVK
                     SVPEGRASFRYEPAKWSVAQVVGHVNDAERIFTTRALCFSRGEKAPLPSFDEKAYAAA
                     AAADQRHIGDIGAEFEAIRRATIALFSGLTESMFAMGGVASGNPITVRALAYIVAGHC
                     FHHARILKERYL"
     gene            complement(659..1744)
                     /locus_tag="VFU75_02395"
     CDS             complement(659..1744)
                     /locus_tag="VFU75_02395"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU5465707.1"
                     /translation="MTTPIPVLALVLLAGCAVATGLPAPQRGSIDALFRDYTGAVPGA
                     SVIVIRDGRVLYEHAYGLANLNPPTPATPETDYRLASLTKEFTALSVMLLAKDGKLRY
                     DEAIGYLLPGLPDRLRPLTVRQLLTHTSGIWDYEDLIPDTQTTQVSDADVLAMISKKD
                     TLYFQPGTKFQYSNTAYVLLGLIVERTSGMTFPRFLQGRVFKPAGMTNTLLFEKGGPP
                     VPTRAFGYTADSGAPTFKATDQSVTSATRGDGGIYSSVLDLAKWSDALEGAQLLDQTR
                     LAVGFTSERLADGTTTGYGFGWYVDTFRGARRLWHHGETSGFRNTIVRFPDLHAAIIV
                     LSNRNGGDTGKIADHIAELLFFPKGAP"
     gene            complement(1741..2100)
                     /locus_tag="VFU75_02400"
     CDS             complement(1741..2100)
                     /locus_tag="VFU75_02400"
                     /inference="COORDINATES: protein motif:HMM:NF015893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC/Spx/MgsR family protein"
                     /protein_id="HEU5465708.1"
                     /translation="MAGPQQVQIFGVKKSAETRAALRFFAERRIKTHFVDLNERPASL
                     GELRRFAQKFGTLELVDRESRRYAELGLSVARLSDERWLEKLSLEPLLLRMPLVRNEN
                     RLTIGLADATWKEWCAA"
     gene            2125..2982
                     /locus_tag="VFU75_02405"
     CDS             2125..2982
                     /locus_tag="VFU75_02405"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5465709.1"
                     /translation="MNPRLKILAAAVLFSTSGAAIKYLTLDVWQLAGLRGLIAGVALL
                     AMLPEARRGWTWRTFLVGSGYAGATITFALANRLTTAASAIFLQSTSPLFILLLAPAL
                     VGERARRRDLGVLAAMAVGMALFFVGMDRPGRVSQNPIAGDLIALLSALTWALAMIGS
                     RWIGRGSNAPGAVAASAAVGNLLSFLFCVPFGPVLTGLDAGQAGILLYLGIFQLALGY
                     VLISKALPEVPALEVSLLLLVEPVVSPVWAFLVVSEVPGMWSLVGGGVILVATVANAR
                     TGVAIPPAD"
     gene            3463..5559
                     /gene="fusA"
                     /locus_tag="VFU75_02410"
     CDS             3463..5559
                     /gene="fusA"
                     /locus_tag="VFU75_02410"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU5465710.1"
                     /translation="MRVYKTEDIRNVAVVGHGASGKTSLVDALAFVAGTSKRHGSVKD
                     GTALTDYTPDEIQHKYSINLALAVAEWMDAKINLIDTPGYLDFTGDALAGVWAADGAV
                     VVLAATAGVEVGTEKVWEYCEERGTPRILFVSMMDKEHADFEKVFNQVKERLTAKVIP
                     VEIPVGEGPEFHGIINLFSKKCHMYKKGTKAGEYEEVDVPPEYQDRFQKYSEALIESI
                     AATDDTLLERYLGGEEIGRDEAITAMKSGMARGELFPLFCGAAELTYGTKALLSKMVE
                     LMPSPKDRPEVEGQVWGNAQKVACAPADQGPLVARVFKTISEPHVGDVSYFRIISGMV
                     KNGQDVYNAPRQAVEKLNHLCVSVGKDRTEVQELHAGDIGCVAKLRDTHTNDSLSTKE
                     HPIVLPKIPFPEAIVTSGIEVKQRGEEDKLSMGLHKLHEEDPTFQHEYSSELGQTLIR
                     GLGERHIEIVVGRLARKFGVHALLTKPRIAYRETLRAKAEGQGKHKKQSGGRGQYGDC
                     WIRLAPMPRGTGVVFEDHIVGGVIPRQYIPAVEKGVEEAAARGPIAGYPVVDFKIELY
                     DGSYHDVDSNEMSFKMAGILAFRNVSPNCKPALLEPIVELSVWTPEDVLGSVMGDLSS
                     RRGHILGSEPDGRLTRVKAHVPEAELDKYATTLHSLTHGRGTYHAQFFKYEQAPPEVA
                     NKIAETRKKELEEAKA"
     gene            5561..>6342
                     /locus_tag="VFU75_02415"
     CDS             5561..>6342
                     /locus_tag="VFU75_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEU5465711.1"
                     /translation="MADLFRFWFGWVTPVGRRRYFLHGAGLMVFKYFVDAGVIWLATG
                     VVWTPLDYFDPVWSLRAAALGQGHELLAFLLAIWTLPFLWIGVSLSMRRALDAGRSAW
                     GVIVFFIPGFNYLLMLVLSVLPSRPARPDGPPRTVDPRLRSALYAVAAGLAITIPTVL
                     LALYERRFYSLGLFLGTPFTLGAICAHVYNRRNPGPFRKTVPVVLLTVMLLGLVLVLF
                     ALEGVVCIAMAFPIAAMAALAGAILGRAIAMKSSEMGASTGMA"
BASE COUNT         1069 a         2006 c         2153 g         1114 t
ORIGIN      
        1 gagcttgagg accgcgaggg attcgccatc ggcgaaatga aagttcgcga gggtgaaggt
       61 agcggtatca ccctgggcct ggatggcggc cggcaagagc agcgcaacga ggacggcagg
      121 caggcggcgc atgagactcc ccgctagagg tagcgttcct tcaggatccg cgcgtggtgg
      181 aagcagtgcc ccgccacgat ataggccagc gcgcgaaccg tgatgggatt gccggaggcg
      241 acaccaccca tggcgaacat cgactccgta agccctgaga acagcgcgat ggtcgcgcgc
      301 cggatggcct cgaactcggc gccgatgtcc ccgatgtgac gctggtcggc ggccgcggcc
      361 gccgcatacg ccttttcgtc gaaactcggc aagggcgctt tctcgccgcg ggaaaagcag
      421 agagcccggg tggtgaagat gcgctccgcg tcgtttacgt gccccaccac ctgcgccacg
      481 ctccacttgg ccggctcgta gcggaaggac gcccgccctt caggaaccga cttcaccagc
      541 gctccgaact ccatgccctg ccgggcgagg atgtcgagga gattcccttt cggaacctgg
      601 tcgatgtact gctgatagta cgggttgaac tcgcctgcta gcggacgctc gatctcgatc
      661 atggggctcc ctttgggaag aagagcagct cggcaatgtg gtccgcgatc ttgcccgtgt
      721 cgcccccgtt ccggttgctc aggacgatga tggcggcatg gagatcgggg aaccggacga
      781 tggtgtttcg aaagccggag gtttccccgt gatgccagag cctccgggct cctcgaaacg
      841 tatccacgta ccagccgaag ccgtatccgg ttgttgtgcc gtcggcgaga cgctccgacg
      901 tgaaccccac ggccaggcgg gtctggtcga gcagctgggc gccctcgagc gcgtcactcc
      961 acttcgcgag gtccaggacc gacgagtaga tcccaccgtc accccgcgtc gcgctggtga
     1021 cgctctggtc ggtcgccttg aaggtcggcg cccccgagtc ggcagtgtac ccaaaagcgc
     1081 gggtcggtac cggagggcca cccttttcga agagcagggt gttggtcatt cccgcgggct
     1141 tgaagacccg tccttgcaag aacctgggga aggtcattcc cgaggtccgc tcgacgatga
     1201 ggccgaggag aacataggcg gtattgctgt actggaactt ggtgccgggc tgaaagtaga
     1261 gcgtgtcctt cttcgagatc atcgccagca cgtcggcgtc cgacacctgc gtggtctgag
     1321 tgtccgggat cagatcctcg tagtcccaga tcccggaagt gtgcgtgagc agctggcgca
     1381 cggtgagtgg ccggagccgg tccggcaatc cgggaagcag atacccgatc gcctcgtcat
     1441 accggagctt gccgtccttg gccaggagca tgaccgacag tgccgtgaac tccttggtga
     1501 gggacgccag ccggtagtcg gtttccggcg tggcgggcgt tggggggttg aggttggcca
     1561 ggccgtaggc atgctcgtag agcacgcggc cgtcccggat gacgatgacg ctagcccccg
     1621 ggacagcacc ggtgtagtcg cggaacaggg cgtcgatgga gccccgctgg ggtgccggca
     1681 gaccggtcgc gacggcacac cccgcgagaa gcacgagcgc cagcaccgga atcggagtcg
     1741 tcacgccgcg caccattcct tccaggtcgc atcggccagg ccgatggtga gccggttttc
     1801 gttgcggacc aggggcatcc tcaggagcag gggctccaga gaaagcttct ccagccaccg
     1861 ttcgtcggac aagcgggcga ccgagaggcc cagctccgca tagcggcggg actcgcggtc
     1921 gacgagctcg agggtcccga acttctgggc gaagcgccgc aactccccca gagaggccgg
     1981 ccgctcgttc aagtcgacga agtgggtctt gatgcgccgc tccgcaaaaa atcgcagggc
     2041 ggcgcgggtt tccgcgctct tcttcacgcc gaagatctgg acttgctggg gaccggccat
     2101 gagagggcaa ttataattcg ggccgtgaac ccgcgcctca agatcctcgc tgccgccgtc
     2161 ctgttctcga cgtccggcgc ggcgatcaag tatctcacgc tcgacgtctg gcagctggcg
     2221 gggctccgtg gcctgatcgc cggcgtcgcg ctcctcgcga tgcttccaga ggctcgccgg
     2281 gggtggacct ggcgcacctt cctggtgggg agcggctacg cgggcgccac catcacgttc
     2341 gctttggcca atcggctcac gacggcggcg agcgcgattt tcctccagtc gacgagcccg
     2401 ctgttcatcc tgctcctcgc cccggcactc gtcggtgagc gcgcccgtcg ccgcgacctc
     2461 ggcgtgctgg cggcgatggc ggtggggatg gccctgttct tcgtcggcat ggaccggccg
     2521 ggccgggttt cccagaaccc gatagcggga gacctgatcg ccttgctctc ggcgctcacc
     2581 tgggcgctcg cgatgatcgg ctcccgctgg attgggcgtg gcagcaacgc tcccggcgcg
     2641 gtggccgcgt cagcagcggt tggtaacctg ctctcatttc tcttttgtgt ccccttcggc
     2701 ccggtcctca ccgggttgga cgccggtcag gccggcatcc tcctctacct gggcattttc
     2761 cagctcgcgc tcggctacgt gctgatctcc aaggccctgc cagaagtccc ggcgctcgaa
     2821 gtgtcgctcc tcctgctcgt ggagcccgtg gtgagcccgg tatgggcgtt tctggtggtg
     2881 agtgaggttc cggggatgtg gtcgttggtg ggaggggggg tcatcctggt ggcgacggtc
     2941 gccaacgcac ggacgggagt cgcgataccg ccggcggact gaggcgtgcc agcgtaacca
     3001 ggaacgcgga gctcggaagt gcgaacgcgg aaccaactca ccggttccgc gttccgcgtt
     3061 cccacttccg cgttcgagtt agatcgcgcg attccggcgg gccgagcggc ggctccggat
     3121 ccgctgtcgc cggtgcgatc tcatccggcg gatcttgcgc tgccgatggc ggaaggaccg
     3181 gccgcctcga aagccgcgag gatgctgccg gacactggcc gccggtggcg cggcggtctg
     3241 tgcttgactc accgcaggcg cggcgacggc caacgcgagc gcagcgagga ctatggtggt
     3301 gatgcgcatg acctctccgt gtgagttctc aatccttcga cgttcaactc cgcgacgcgt
     3361 taaccgtcag cgaaagcctt gccagatcgg ggcttcccgc gtctagccgg gctcaatcct
     3421 cgcggccata ttcgcaggaa ttccgcccta ggaggcatac gcatgcgcgt gtacaagacg
     3481 gaggacatcc gcaacgtggc cgtggtgggc cacggcgcga gcggcaagac ctccctggtc
     3541 gacgcactcg cgttcgtggc cggaacgagc aagcgccacg ggtccgtgaa ggacggcacc
     3601 gccctcaccg attacacccc cgacgagatt cagcacaagt actccatcaa cctggcgctg
     3661 gcagtggccg agtggatgga cgccaagatc aacctcatcg acacgcccgg atacctggac
     3721 ttcaccggcg acgcactcgc cggcgtctgg gccgccgacg gcgcggtggt ggttctggcg
     3781 gccacggccg gcgtggaggt cggcaccgaa aaggtgtggg agtactgcga agagcggggt
     3841 acgccccgca tcctgttcgt gtcgatgatg gacaaggagc acgcggactt cgagaaagtc
     3901 ttcaatcagg tgaaggagcg gctgaccgcg aaggtgatcc cggtggagat tccggtgggt
     3961 gaaggccccg agttccacgg gatcatcaac ctgttctcga aaaagtgtca catgtacaag
     4021 aagggcacga aggccggcga gtatgaagag gtcgacgttc cccccgagta ccaggatcgc
     4081 ttccagaagt attccgaagc gttgatcgag agcatcgccg ccacggacga caccctgctc
     4141 gagcggtact tggggggtga ggagatcggc cgggacgagg cgatcacggc catgaagtcg
     4201 gggatggcgc gcggggagct gttcccgctg ttctgcggag ccgccgagct gacttacggg
     4261 acgaaggcgc tcttgagcaa gatggtcgag ctcatgccgt cacccaagga tcggcccgag
     4321 gtggagggcc aggtgtgggg gaacgcgcag aaggtggcct gcgcgcccgc cgaccagggg
     4381 cccctcgtgg cgcgggtgtt caagaccatc tcggaacccc acgtcggcga cgtctcctat
     4441 ttccggatca tctccggaat ggtcaagaac ggccaggacg tgtacaacgc gccgaggcag
     4501 gcggtcgaga agctcaatca cctctgtgtt tcggtgggga aggaccggac cgaagtccag
     4561 gagctccacg ccggcgacat cgggtgcgtg gcgaagctga gagacaccca cacgaacgac
     4621 agcctgtcca ccaaggagca cccgatcgtg ctgcccaaga tcccgttccc ggaggccatc
     4681 gtgacttcgg ggatcgaggt caagcagcgg ggagaggaag acaagctctc gatggggctc
     4741 cacaagctcc atgaagaaga tccgaccttc cagcacgagt actcctccga gctcgggcag
     4801 acactcatcc gcgggctggg tgagcgtcat atcgagatcg tggtcggccg gctcgcccgg
     4861 aagttcgggg tgcatgccct gctcacgaag ccccggatcg cataccgtga gacgctccgg
     4921 gcaaaggccg aggggcaggg gaagcacaag aagcagtcgg gtgggcgggg acagtacggg
     4981 gattgctgga ttcgtctcgc cccgatgccg cgtggcactg gtgtggtgtt cgaagatcac
     5041 atcgtgggcg gtgtgatccc gcgtcagtac atcccggccg tcgaaaaggg cgtcgaggag
     5101 gcggccgcgc gaggtcccat cgcgggctat ccggtggtcg acttcaagat cgagctgtac
     5161 gacgggtcat atcacgacgt ggattcgaac gaaatgtcgt tcaaaatggc cgggatcctg
     5221 gctttcagaa acgtgagtcc caactgcaag ccggcgctgc tcgagcccat cgtcgagctc
     5281 tcggtgtgga cgcccgaaga cgttctcggc tccgtgatgg gggacctgtc ttcgcgccgc
     5341 ggccatatcc tggggagtga gcccgacggg cgactcaccc gcgtgaaggc ccacgtgcct
     5401 gaggcggagc tcgacaagta cgcgacgacg ctccactcgc tcacgcacgg caggggtacg
     5461 taccacgcgc agttcttcaa gtatgagcag gcgcctccgg aggtcgcgaa caagatcgcg
     5521 gagacccgca agaaggagct ggaagaggcc aaggcctagc gtggccgatc ttttccgatt
     5581 ctggtttggc tgggtcacac ctgtcggccg gcgccggtac ttccttcacg gcgccggcct
     5641 catggttttc aagtacttcg tggacgccgg agtcatctgg ctcgccaccg gcgtggtatg
     5701 gacccccctc gactactttg accccgtttg gtccctgcga gccgccgcgc tcggccaggg
     5761 acacgagctg ctcgcgttcc tgctggcgat ctggaccttg ccgttcctct ggatcggggt
     5821 gtccctcagc atgcgccggg cgctcgacgc cggccgctcg gcctggggcg tcatcgtctt
     5881 cttcatcccg ggtttcaact acctgctgat gctggtgctc agcgtcctgc ccagccggcc
     5941 cgcgcgtccc gacggaccac ctcggaccgt ggatccgcgg ctgcggagtg ccctctacgc
     6001 cgtggcggcc ggactcgcga tcaccattcc cacggttctg ctggcgctct acgaacgccg
     6061 cttctactcg ctgggactgt tcctgggtac cccattcacg ctcggcgcca tctgcgcgca
     6121 cgtctacaac cgcaggaatc ccggcccatt ccggaagacg gtccccgtgg tgctcctgac
     6181 cgtcatgctg cttgggctgg tgctggtgct cttcgctctc gaaggcgtcg tctgtattgc
     6241 catggcattc ccgattgccg ccatggctgc gctcgccggg gcgatcctgg gccgggccat
     6301 tgcgatgaag tcgtccgaga tgggcgcgtc caccgggatg gc
//
LOCUS       DASUBP010000055         3335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_151075, whole genome shotgun sequence.
ACCESSION   DASUBP010000055 DASUBP010000000
VERSION     DASUBP010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3335
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151075"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..249
                     /locus_tag="VFU75_02420"
     CDS             <1..249
                     /locus_tag="VFU75_02420"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5465712.1"
                     /translation="LNLEAALRDAARVRLRPILMTTLCTLFGLLPLALGLGAGSELQR
                     PLALTVIGGLALSTPITLYAVPALLVAIRGGDATLSRV"
     gene            316..2247
                     /locus_tag="VFU75_02425"
     CDS             316..2247
                     /locus_tag="VFU75_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:574..576,aa:Sec)
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU5465713.1"
                     /translation="MVQPKNAHGYVRLTEPLVRDGGTLRPATWAEALDRAAEGFRRNL
                     KPGPNRLGIFSCSKSTNEMNFLAGKLARVAFGTHNVDSCNRTUHAPSVVGLATVFGAG
                     GGTTSYREVEETEVLFLWGANARENHPIFFHHVLKAVHRGARLYAVDPRRTTSAQWAD
                     RWLGLHVGSDIALANAMARVIIHAGLADEQFIKNATTGFEEYRKCVEAYTLDRAERLT
                     GVPAAAIEEAALAYGRAKRAVICWTLGITEHHNAVDNVLALINLSLLTGHVGRYGSGV
                     SPLRGQNNVQGGGDMGAIPNKLVGFQEIRDAAIRARFEKAWSVTLQPDHGWTITQMFE
                     AMDHGDLTGLYVIGENPAQSEADQHRTQRLLDGLDHLVVQDIFLTRTAEMADVVLPAV
                     ATWCEGEGTVTNSERRVQRVRPALAPPGDARDELWILAQIAKRMGHDWGEPTAKSVWE
                     EVRTLAPEMFGGMSYERLEALGGIQWPCPDESHPGSLFLHERLWAEPRGGPAAPFSVV
                     EHEPPVEAPDAEYPFVLTTGRRLDSYNTGVQTMGYASPLRRPEALDMSPEDAAEMGLA
                     EGDSVTVASRRGSVTAPVHVEPGLRPGLVFMTLHNQDDVRVNLLTIDATDPRSGTAEF
                     KACAVRVTPAATAAAVASR"
     gene            2254..>3335
                     /locus_tag="VFU75_02430"
     CDS             2254..>3335
                     /locus_tag="VFU75_02430"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEU5465714.1"
                     /translation="MDLHLRDATPTEAERAAVDGVLGPPSSGWDGGERVAGRDGHLAV
                     GGHEARARRHLLLPALHAVQSRTGWLSEGALNYLSARLTVPLAEIWGVVTFYALLPTH
                     PRPPRVLHVCDDIACRMRGANGICERLEHDVGAPVTDAGHGETSEAMDPGEHGWLKSP
                     CLGLCDQAPAALLTVADEKPIERLLGNVTPTAAVAALRGVIPDELRARLSQKGTPGLA
                     LLARVGVVDPASLRAYQGAGGYRALAKAFELKPEGVINEMTASKLMGRGGAAFPTGRK
                     WDAVAKQGARPHYVICNADESEPGTFKDRVLMEEDPFAIVEAITIAGYAVGAEQGYVY
                     IRGEYPLAIRRIQGAIDQARQAGLLGP"
BASE COUNT          529 a         1082 c         1184 g          540 t
ORIGIN      
        1 ctgaatctgg aggccgctct ccgggatgcc gcgcgcgtgc gattgcgtcc cattctgatg
       61 acgacgctct gcaccctctt cggtctgctg ccgctggccc tcggcctcgg ggcggggagc
      121 gagttgcagc ggccgttggc ccttaccgtg atcgggggcc tcgccttgtc gacgccgatc
      181 accctgtatg ccgttcccgc gctgctggtc gccattcgcg gcggggacgc gaccctctca
      241 cgagtctaga cctccgcccc agcttcctct aggttagtcc atacgaccaa ctctctatga
      301 gagaggcgag tctctatggt gcaaccgaag aacgcccacg ggtatgtccg gctgacggag
      361 ccgctggtgc gcgacggggg gacattgcgt cccgccacgt gggccgaggc actcgaccgg
      421 gcggccgagg gattccggcg gaatctgaaa cccggaccca accggctggg catcttcagc
      481 tgttccaagt ccaccaacga gatgaatttc ctcgccggga aactggcgcg ggtggccttc
      541 ggaacgcaca acgtcgacag ctgcaaccga acgtgacacg ctcctagcgt cgtcggtctg
      601 gcgacggtgt tcggtgcggg tgggggaacc acttcgtacc gggaggtgga agaaaccgag
      661 gtcctgttcc tctggggtgc caatgcccgg gagaaccacc cgatcttctt ccatcacgtg
      721 ctcaaggcgg tgcaccgggg ggcccggctc tatgccgtgg acccgcggcg gacgacgtcc
      781 gcccaatggg cggaccgctg gctgggcctc cacgtgggtt cggatatcgc gctcgccaat
      841 gcgatggcac gcgtcatcat tcacgccggt ctcgccgacg aacagttcat caagaatgcg
      901 accacgggat tcgaggaata ccggaagtgc gtcgaggcgt acacgctcga ccgggccgag
      961 cggctcaccg gcgtccccgc tgcggccatc gaagaagcgg cgctggcgta cgggcgggcc
     1021 aagcgcgcgg tcatctgctg gacgctgggg atcaccgagc accacaatgc ggtggacaac
     1081 gtgctcgcgc tcatcaatct gtccctgctc accggacacg tgggccggta cggttcgggg
     1141 gtgagcccgc ttcgcggtca gaacaacgtg cagggtggcg gggacatggg tgccatcccc
     1201 aacaagctcg tggggttcca ggagatccgg gacgccgcca tccgggcacg gttcgagaag
     1261 gcctggagcg taacgctgca gcctgatcac gggtggacca tcacgcagat gttcgaggcg
     1321 atggaccatg gtgatctcac cgggctctac gtcatcggtg agaacccggc ccagtcggaa
     1381 gccgaccagc accgcacgca acgcctgctc gacggactcg atcacctcgt ggtgcaggac
     1441 atcttcctga ccaggacggc ggagatggcc gatgtggtgc tccccgcggt cgcgacgtgg
     1501 tgcgaagggg aagggaccgt cactaacagc gagcgccggg tgcagcgcgt gcggccggcg
     1561 ctggcgccgc cgggtgatgc ccgtgacgag ctgtggatcc tggcgcagat cgcgaagcgg
     1621 atggggcacg attggggtga gcccaccgcg aaatcggtgt gggaagaagt gcggacgctc
     1681 gcgcccgaga tgttcggcgg gatgagttac gagcgactcg aggcgctggg gggcatccag
     1741 tggccctgtc cggacgaatc ccatccgggc tcgcttttcc tgcacgagcg tctgtgggcc
     1801 gagccgcgtg gggggcccgc ggcgcccttc tcggtggtgg agcacgagcc gccggtcgag
     1861 gcgcccgacg ccgagtatcc gttcgtcctg accacgggac gtcgcctcga ttcgtacaac
     1921 accggggtgc agaccatggg gtacgcttcc cccctgcgcc ggccggaggc gctcgacatg
     1981 tctcccgagg acgcggcgga gatggggctc gccgagggag attcggtgac ggtggcttcg
     2041 cggcgcggga gcgtgacggc ccccgtgcac gtcgaacccg gactccgccc gggattggtc
     2101 ttcatgacct tgcataacca ggatgacgtg cgcgtgaacc tgctcaccat cgacgcgacc
     2161 gatccacggt cgggaaccgc cgagttcaag gcctgcgcgg tgcgtgtgac gcccgccgcg
     2221 acggcggcag ccgtcgcgag ccgctgaggt ccagtggatc ttcacctccg cgatgccacg
     2281 ccgaccgaag cagagcgggc tgccgtcgac ggcgtcttgg gcccgccgtc ttccgggtgg
     2341 gatggtggag agcgcgtggc gggccgcgac ggtcacctgg ccgtcggggg gcatgaggcc
     2401 cgtgcccgcc gccacctgct gctgcccgct ctgcacgcag ttcaatcccg aaccggttgg
     2461 ctctccgaag gtgctctcaa ttacctgagt gcgcgcctga ccgtgccgct ggccgagatc
     2521 tggggtgtgg tgacgttcta cgcgctcctg ccgacgcatc cgcgtccacc gcgtgtgctc
     2581 catgtctgcg acgacatcgc ctgccgcatg cggggcgcga atgggatctg cgagcggctg
     2641 gagcatgacg tcggcgcgcc ggtgaccgac gccggtcatg gagagacgtc cgaggccatg
     2701 gaccccggcg agcacggctg gctcaagagt ccctgtctgg gactgtgcga tcaggctccg
     2761 gcggccttgc tgaccgtcgc ggacgaaaag ccgatcgagc gactcctcgg gaacgtgact
     2821 cctaccgccg cggtggccgc gctccggggg gtgatccccg atgaactgcg tgccaggctt
     2881 tcccagaagg gaacgcccgg tctcgctctg ctcgcccgtg taggggtcgt ggatcctgcc
     2941 agtcttcggg cgtatcaggg ggccggcggg taccgggcgc tggcgaaagc gttcgagctc
     3001 aagcccgagg gcgtgatcaa cgagatgacc gcctccaagc tcatggggcg cggcggcgcg
     3061 gcgtttccga ccggccggaa gtgggacgca gtcgccaagc agggggcccg accgcactac
     3121 gtgatctgca acgcggacga gtcggagccg ggcaccttca aggaccgggt tctgatggaa
     3181 gaggatccgt tcgcgatcgt cgaggccatc acgatcgccg ggtatgcggt gggcgcggag
     3241 cagggctacg tctacatccg gggcgagtac ccactcgcca tccgccgcat acaaggggcg
     3301 atcgatcagg cccgtcaggc ggggctgctc ggtcc
//
LOCUS       DASUBP010000056         5495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_396407, whole genome shotgun sequence.
ACCESSION   DASUBP010000056 DASUBP010000000
VERSION     DASUBP010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5495
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_396407"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VFU75_02435"
     CDS             complement(<1..293)
                     /locus_tag="VFU75_02435"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEU5465715.1"
                     /translation="MAIACFIRYEIDPFQLDAFAQYATIWLTAIPRLGGNLLGYFLPH
                     EGTNNVAWGIVAFDSLALYEAYRTRVKADPEARANFAFAHDRRLILREERTFLR"
     gene            complement(371..1978)
                     /locus_tag="VFU75_02440"
     CDS             complement(371..1978)
                     /locus_tag="VFU75_02440"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEU5465716.1"
                     /translation="MIGVVRPAGDLLLEARGRERAGCIPEAVSAYETAITAAEKAGDT
                     KVLAEALRRLAVVRHNQGDSGTGRDLVGRSHNVARANGDALLAAEALNTLGGIELQSG
                     ALEAARAALNQALEEGGQFQALRARVQQHLGIISNIQGDLEAALSHYEFSLESYQGAN
                     DEHGCAIAYHNLGMISADRDLLPDADRYFTQSLEIAQRAGDVSLQGLCLVNHADVDLQ
                     RQMFEEARHKAEAALALFDQLGDRGSKSTAYRVIGMVYRETGKAPLAESRLKSSIELA
                     AGVGYVLSEAESTRELAILYQGMGRNQEALTLLNRSYGLFQRLDARVDLVNVGGKVAD
                     LEGTYLVVVRNWGQSIESTDTYTYGHCERVANYAAAVARHAGLDDVARTTIRLGAYLH
                     DVGKVKVPHEILNKPGPLTRDEFEVVQMHPVWGVELLASVEFPWDLKPIIRWHHERYD
                     GTGYPDHLKGDEFPLSAQVVGIVDVYDALTTDRPYRPGMSHETALAEITKCRHWWSTQ
                     VFDAFLAALPEIKEAAKKNFELIAAVA"
     gene            complement(1983..2534)
                     /locus_tag="VFU75_02445"
     CDS             complement(1983..2534)
                     /locus_tag="VFU75_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465717.1"
                     /translation="MRSTRLLLTAALVFVFASCTEQGPLGVTADQPASGAATKVAAGP
                     QHDLLGGLLQSVGLLKCKPLAPATTTATIGPLGGSLKVGPHTFTVPAGALSQNVTITA
                     SLSADTINAITFKPEGLRFAKSASLTMSYANCNLVGILLPKQIAYTSDNYTILSLLAS
                     VDNILTQKVTGQVWHFSHYAMAW"
     gene            complement(2736..3689)
                     /locus_tag="VFU75_02450"
     CDS             complement(2736..3689)
                     /locus_tag="VFU75_02450"
                     /inference="COORDINATES: protein motif:HMM:NF036967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HEU5465718.1"
                     /translation="MSNPPSHPRRREVPKDLGFGSVVSDESRLRLLNRDGSFNVRRTG
                     LKYFESMSLYHTLLTISWPKLLSLISAFYIAVNLFFGALFWSLGPTALNGTSAAMPEG
                     SYLRAFFFSVQTFGTIGYGQVGPVGLVPNLLVTLESLCGLMALALITGILFARFSRPT
                     AAIMFSNKALIAPFGEVKAFEFRITNRRTNQLIDLHARVLFSRMRHREGRAIRDYAEL
                     TLERASVVFFPLAWTVVHVIDENSPLNGSSAEQLAAEEAEFLVLLEGTDETFAQRVHA
                     RTSYKPTEIVWNARFVDMFDRKGLEDGLSIDIGRLHDFQTV"
     gene            3841..>5495
                     /locus_tag="VFU75_02455"
     CDS             3841..>5495
                     /locus_tag="VFU75_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465719.1"
                     /translation="MIPLTSTSEVFVPPKGRAFEKLSFDFPEPSVSFAGLRFGFLVFS
                     RENIYGLDLGRTVVEPAGDHAVTLTCTGFVWAGGQERAAGHLVARIRENRDNSIEWDV
                     TVDFDRPIKAVTAIVRGVPRGKISGGGNTPYDPHDDEVLIGYPFGAGDLFGGNTADSM
                     STPFAAIVKENGSCWAFSSLDDKVRAKRLFFQPGESSYRVEAVFETEGWLNQTSVTVP
                     TWRLSRDVSLDDAVAEHYAHVEKAYQIPSIDTRPDAPAWLKDVALVLTLHGAHYTGFL
                     FNDFAKMREILNWVAGEIPPNRVLVFLPAWDGRYYWNYPLYQADDRMGGEAGLKALIK
                     GGQAQGFRFMPMFGANCANRKHPEWAKFADARAERIDGDAFDENWVDWDGDRHQDGNI
                     SYMNLGVDSWRNWLEARIADCIDRYGVEAYFLDITGGWINTPQADMNEGLKRLVSDLR
                     AKYPKVLCCGEFHYDALLSFIPFYHVASRRAAKYARFFSHLSHPAPGRGSSGVHESGF
                     SQFDSATLSLSPGTIPTLQVVDDTFTKQRSVMKELIARAKERAG"
BASE COUNT         1005 a         1735 c         1792 g          963 t
ORIGIN      
        1 cgaaggaatg tccgttcctc gcgcagaatc agccgtcggt cgtgcgcaaa cgcgaaattg
       61 gcgcgggcct caggatccgc tttgacccgg gtgcggtagg cctcatagag cgccaacgaa
      121 tcgaaggcga cgatgcccca ggccacgttg ttcgtgccct cgtggggcag gaagtagccc
      181 aggaggttgc cgccgagacg gggaatggcc gtgagccaga tcgtggcgta ctgggcgaag
      241 gcgtcgagct gaaaggggtc gatttcgtag cggatgaaac aggcgatggc cacggtcccg
      301 tgccggctgg agatgaacgc atcctagccg gaggggtgct gcgacgattc tatggcgggc
      361 gaaacgtcga ttacgcgacg gcggcgatca actcaaagtt ttttttcgcg gcttccttga
      421 tttcaggcag cgccgcgagg aaggcgtcga acacctgcgt cgaccaccag tggcggcatt
      481 tcgtgatctc ggccagcgcc gtctcgtggg acatcccggg acggtaaggg cggtcggtgg
      541 tcagggcgtc gtacacatcc acgattccca cgacctgggc cgacagcgga aactcgtccc
      601 ccttcaagtg gtccgggtaa ccggtcccat cgtaacgctc gtgatgccag cgaatgatcg
      661 gcttcaggtc ccacgggaat tccacggagg ccaggagctc cacaccccac acggggtgca
      721 tctggaccac ctcgaattcg tcccgggtga gcggtcccgg cttgttcagg atctcgtgcg
      781 ggaccttcac cttccccacg tcgtggaggt aggcgcccag gcgaatggtg gtgcgagcga
      841 cgtcgtcgag cccggcgtgc cgggccacgg cggccgcgta gttcgccacc cgctcgcagt
      901 ggccgtacgt gtaggtgtcc gtggattcga tcgactggcc ccagttccgg accacgacga
      961 gataggtgcc ttcgaggtcg gcgaccttgc cgccgacgtt gaccagatcg acccgggcgt
     1021 ccaggcgctg gaacaacccg tacgaccggt tcaggagcgt cagggcttcc tgattccgcc
     1081 ccatcccctg gtagaggatc gccagctcac gggtggactc cgcctcggac aacacataac
     1141 cgactccggc ggcgagctcg atggacgact tgagccgcga ctccgcgaga ggagccttgc
     1201 cggtctcccg gtagaccatc ccgatgacgc ggtaggcggt ggacttggag ccacggtccc
     1261 cgagctggtc gaaaagcgcc agcgccgcct cggccttgtg gcgcgcttcc tcgaacatct
     1321 ggcgctggag atccacgtcc gcgtggttca cgaggcagag cccctgcagg ctcacatccc
     1381 ccgcgcgttg cgcgatctcg aggctctggg tgaagtaccg gtcggcatcc ggaaggagat
     1441 cccggtccgc gctgatcatt ccaagattgt ggtaagcaat ggcgcagcca tgctcgtcgt
     1501 tcgcgccttg gtaggattcg agcgagaact cgtagtggct gagcgccgcc tccaagtccc
     1561 cctggatgtt cgagatgatg ccgaggtgct gctgcacccg agcccggagg gcctggaact
     1621 gtccgccttc ctcgagcgcc tgattcaggg cggcgcgggc cgcttcgagg gcgcccgact
     1681 gcagctcaat cccccccagc gtgttgagcg cctccgcggc gagcagtgcg tcgccgttgg
     1741 cccgtgccac gttgtgactc ctcccgacca agtcgcggcc ggtgccggag tccccctggt
     1801 tgtgccgtac caccgccagc cgccgcagcg cctcggccag caccttggta tcgccggcct
     1861 tttcggcggc tgtgatcgcc gtttcgtacg cgctgactgc ctcggggatg caccccgccc
     1921 gctcccgccc ccgtgcttcg agcagcaaat caccagccgg gcgcacgacc ccgatcatgt
     1981 tcttaccacg ccatcgcgta gtgagagaag tgccagacct ggcccgtcac cttctgggtg
     2041 aggatgttgt cgaccgaggc cagcagcgac agaatcgtgt agttgtcgct cgtgtacgcg
     2101 atctgcttcg gcaggagaat gccgaccagg ttgcagttcg cgtagctcat ggtcaacgac
     2161 gccgacttgg cgaagcgcag gccttccggc ttgaaggtga tcgcgttgat ggtgtcagcc
     2221 gagagcgacg ccgtgatcgt cacgttctgc gacagggcgc ccgcgggcac cgtgaacgtg
     2281 tgcgggccga ccttcagcga tccgccgagc ggtccgatgg tcgcggtggt tgtcgccgga
     2341 gcgagcggct tgcacttgag cagtccgacc gactgcagca ggccaccgag caggtcgtgc
     2401 tgcggaccgg ccgcgacctt ggtggctgca cccgaggccg gctgatcggc cgtgacgccg
     2461 agcgggccct gttcggtgca gctggcgaag acgaacacca aggctgcggt caacaaaagg
     2521 cgcgtggacc tcatacaaca ctccagattg ggggtgggct gacgacatgt gcgtatcgca
     2581 ggccacgtac ctcactggat tgtgagacag gtcacaagac ctcggtgaca gtttttctga
     2641 agcaaattta aagatggtga aactgggggt ttcagccttg gaaaaccgtg gtgtttttgc
     2701 aacaggtggt gttgggagga cgccatccag aggtcttaca ctgtctgaaa atcatgcagt
     2761 ctgccgatat cgatcgacaa cccatcctcc agtcctttcc ggtcgaacat atccacgaaa
     2821 cgggcattcc agacgatctc tgtgggcttg tacgacgtcc gggcatgcac ccgctgggca
     2881 aacgtctcgt cggtcccctc gagcaacacc aggaactccg cttcctcggc ggccagctgt
     2941 tcggcagacg agccattcag cgggctgttc tcgtcgatca cgtgcaccac ggtccacgcc
     3001 agggggaaga acaccacgct cgcacgctcc agcgtgagct cggcgtagtc gcggatggct
     3061 cggccttcgc ggtgccgcat ccggctgaac agcacccggg cgtggagatc gatcagctgg
     3121 ttggtccgcc ggttggtgat ccggaactcg aaggccttga cctcgccgaa cggggcgatc
     3181 aacgccttgt tcgaaaacat gatcgcggcc gtgggacggg agaaccgggc gaacagaatc
     3241 cccgtgatca gcgcgagggc catcaggccg cacaaagact cgagtgtgac cagcaagttg
     3301 ggcacaaggc ccacgggtcc gacctggccg tacccgatgg tgccgaaggt ctggacacta
     3361 aagaagaagg cacggaggta cgacccttcc ggcatggccg ccgaggtccc attcaaggcc
     3421 gtggggccga gggaccagaa gagggccccg aagaacaggt tcacggcgat gtagaacgcc
     3481 gagatgaggg acaggagctt gggccaggag atagtgagca gggtgtggta caggctcatg
     3541 gactcgaagt acttgagccc ggtgcgtcgc acgttgaacg agccgtcgcg gttcaggagc
     3601 cgaaggcggc tctcgtcaga tacgaccgat ccgaacccga ggtccttggg aacctcgcgc
     3661 cggcgcgggt gactcggggg attgctcacg aggccaattc tacatagctc tgtccaaatg
     3721 agcgacctga cccgcaggga ttggctgaag gcggtgagcg tggcggcgag tacggcaccc
     3781 gttctgggcg cggagtcgac agctcccggc tcacggctcc cgacgaggcc cccgccccaa
     3841 atgatccccc tcacttcgac gagcgaagtc ttcgtccccc cgaagggccg ggccttcgag
     3901 aagctctcgt tcgactttcc tgagccctcg gtctcgttcg cagggctgcg gttcggcttt
     3961 ctggtcttct cgcgagagaa catctacggc ctggacctgg gccggacggt ggtcgagccc
     4021 gccggtgatc atgccgtcac gctcacctgc acggggttcg tctgggccgg cgggcaggag
     4081 cgcgcggcgg ggcacttggt ggcgaggatc agggagaatc gagacaacag catcgaatgg
     4141 gacgtcaccg tcgacttcga tcggcccatc aaggcggtca cggccatcgt gcgcggagtc
     4201 ccccgcggga agatctccgg aggcgggaac actccctacg acccgcatga cgacgaagtc
     4261 ctgatcggct atccgttcgg cgccggagat ctcttcggcg ggaacaccgc ggacagcatg
     4321 agcacgccat tcgcggcgat cgtgaaggaa aacgggtcat gctgggcgtt ctcgtccctc
     4381 gacgacaagg tccgggccaa gaggctcttc ttccagcccg gcgagtcctc gtaccgagtg
     4441 gaagccgtgt tcgaaaccga agggtggctc aaccagactt cggtgacggt gccgacctgg
     4501 cgcctatcgc gggatgtctc gctcgatgac gccgtggccg agcactatgc ccacgtcgag
     4561 aaggcgtacc agatcccgtc catcgacact cgcccggacg cacccgcctg gctcaaggac
     4621 gttgcgctcg tcctcacgct ccacggcgct cactataccg gcttcctctt caacgacttc
     4681 gcgaaaatga gggaaatcct caattgggta gcgggcgaaa tcccgcccaa ccgggtgctg
     4741 gtatttctgc ccgcgtggga tggccggtac tactggaact accccctcta tcaggccgat
     4801 gaccggatgg gaggggaggc gggcctcaag gcgctgatca aaggaggcca ggcccagggg
     4861 ttccggttca tgccgatgtt cggcgcgaac tgtgcgaacc ggaaacaccc cgagtgggca
     4921 aagttcgccg acgcccgcgc cgagcgtatc gacggcgacg cgttcgacga gaactgggtg
     4981 gactgggacg gagaccggca ccaggacggc aacatctcct acatgaatct gggcgtcgac
     5041 tcctggagaa actggctcga agcccggatc gccgactgca tcgaccgcta tggcgtcgaa
     5101 gcctacttcc tggacatcac cggcggatgg atcaacacgc cccaggcgga catgaatgaa
     5161 ggactcaagc gcctggtgtc ggaccttcga gccaagtacc cgaaagtcct ctgttgcggc
     5221 gagttccact acgacgcgct gctctctttc attcctttct atcacgtcgc cagccgccgg
     5281 gccgcgaagt atgcccgatt cttctcgcat ctctcccatc cggcgcccgg ccggggaagc
     5341 tcgggggtgc acgaatcggg gttcagccag ttcgattccg caacgctgtc cctctccccg
     5401 ggcaccattc ccaccctgca ggtggtggac gacacgttta cgaagcagcg ttccgtgatg
     5461 aaggaattga tcgcgcgggc caaagagcgc gccgg
//
LOCUS       DASUBP010000057         4256 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_614455, whole genome shotgun sequence.
ACCESSION   DASUBP010000057 DASUBP010000000
VERSION     DASUBP010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4256)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4256)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4256
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614455"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="VFU75_02460"
     CDS             <1..1026
                     /locus_tag="VFU75_02460"
                     /inference="COORDINATES: protein motif:HMM:NF009802.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic amidase"
                     /protein_id="HEU5465720.1"
                     /translation="ECTDANITVPFAQRRHFAMPMGTISSSPDTVGVAVVNYEVPVCE
                     SKQEVLENCQRISRMVWGTKHGFPGLDLIIFPEYSTQGFHPTKWRDLTTTADGPEVEI
                     FSKACVDNKVWGIFSLTGEENPAGNPWNSLLLINDKGEVVLNHHKVLPWVPMEPWYPG
                     DTTTVGEGPKGLQVGLVICYEGNHPEIFRDTVMKGAELVVRCQGYMYPSKDQQSIVQQ
                     CRAWENLTYVAVANMAGRDLVYSYFGHSMIVDFDGSVLAECGTAPDEITYALLSVPGI
                     RDARKNWTAENHLFNTLHRGYTALPPDGKAECPFDFYKLWVNEPQRAKELVEALTRPT
                     AIPAETR"
     gene            1232..3502
                     /locus_tag="VFU75_02465"
     CDS             1232..3502
                     /locus_tag="VFU75_02465"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5465721.1"
                     /translation="MRSQPHPHGPADPVTAAPGPIAAQASATEHPAGGSSLLLRVAEA
                     PLDDVDRGVIRADPYDLARIGARPGDVVVIAGERRTAAIAHALPPHLHGTHQILMDGM
                     VRENARAVVGERVDVSAPQAEPARTVLVESLDGGSFGPREIADIREWMSGRAVMYGDR
                     LKVTAFSKRGHMFIVRGTEPEAVVVAGPYTDVRLKKEPATPLVESPSLRVKYEDIGGL
                     DEELLRVRELVELPLKYPSLFARLKIEPPKGVLLYGPPGSGKTLIARAVASEVKAHFI
                     HVNGPEIIHKFYGESEARLREIFEEAQRRAPAVVFLDEIDAIAPRRQDVSGEVEKRVV
                     AQLLALMDGLVPRGQVVVIGATNLPEAVDPALRRPGRFDREITMNVPTRPGRLRILKI
                     HSRGMPLADDVDLEALAEVTAGYVGADLEVLCKEAGMLALRDCLTAAGTQSDTAADPE
                     LAAEQTKIDAQHFHEALKTIEPTATRELALERPNVRWEQIGGLRDVRQTLLSALELPH
                     QHPGLFAQAGIKPPTGFLFEGPSGTGKSLTVTALAAETGMRLISVDAAYLFSKWVGES
                     EKALRTVFKKAKQSAPCILFFDDLQTFAPARRALRDTDSGFAERLARQFFAELDKTIG
                     LGDVIVIGATNRPDLVDPVALRPGRFGFVVHFPLPGTGQREEIFRVHLRQVRLAPDVD
                     VRALAEETPGFSGADIAGVCERALLSEIEAFVRRHGDRADEEAARRSLILSAGALREA
                     LAQARAALPPTAQPEE"
     gene            3506..3784
                     /locus_tag="VFU75_02470"
     CDS             3506..3784
                     /locus_tag="VFU75_02470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465722.1"
                     /translation="MPTCPKCSQPMAPDQVITIAGIRDKAVSWQCPSDHIEAVAVHPV
                     QMAHLHIGQEEEDGGRLTRLIHMDMVGAELILFREIVPLAGKGGEHHG"
     gene            3777..>4256
                     /locus_tag="VFU75_02475"
     CDS             3777..>4256
                     /locus_tag="VFU75_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465723.1"
                     /translation="MADRSALPLDQAQYAAYLAELAEIVHRDPQLEGTAALRAFVETH
                     QEWFGPRTARTTLSTDDTTLRTLIHIMVLAASELADLHPASRTWLTEHGRGLPPWDVT
                     VPRTAQRLVTFRSKVYGVVEWQPEPRIELAPSLTEQERRWATALAIAIGERPQWSSHE
                     "
BASE COUNT          757 a         1459 c         1415 g          625 t
ORIGIN      
        1 gagtgcaccg acgcgaacat caccgttccc ttcgcgcaaa ggaggcactt cgcgatgcca
       61 atgggaacca tttcatcctc gcccgacacg gtgggcgtgg ccgtcgtcaa ctacgaggtc
      121 ccggtgtgcg agagcaagca ggaagttctg gagaactgcc agcggatctc ccgcatggtg
      181 tggggaacca agcacggatt ccccggcctg gacctgatca tcttccccga atactcgacc
      241 cagggcttcc acccgaccaa gtggcgcgac ctgaccacca cggccgacgg cccggaggtc
      301 gagatcttct ccaaggcatg cgtggacaac aaggtgtggg ggatcttctc gctcaccggt
      361 gaagagaatc ccgccggaaa tccatggaac agcctgctgc tgatcaacga caagggcgag
      421 gtagtcctca accaccacaa ggtgctgcca tgggtgccga tggagccctg gtatcccgga
      481 gacaccacga ctgtgggcga aggtcccaag ggcctgcagg tcggcctcgt catctgctac
      541 gagggcaacc atccggagat cttccgcgac accgtgatga agggcgccga actggtggtg
      601 cgctgccagg gctacatgta cccgtccaag gaccagcaga gcatcgtcca gcagtgccgg
      661 gcctgggaga acctcaccta cgtggcggtg gcgaacatgg ccggccgcga cctggtgtac
      721 tcctacttcg gccactcgat gatcgtcgac ttcgacggga gcgtcctcgc cgaatgcggg
      781 acggcgccgg acgagatcac ctacgctctg ctgtctgtac caggcatccg ggacgcgcgc
      841 aagaactgga cggcggagaa ccacctgttc aacaccctgc accgcgggta cacggcgctg
      901 ccgccggacg gcaaggccga atgcccgttc gacttctaca agctgtgggt gaacgagccg
      961 cagcgcgcca aggaactcgt cgaggcgctg acccgtccga cggcgattcc ggccgagacg
     1021 aggtgacggg gacctgatcc ttccaccgct cggtagcggg gggaggggtc gtcgtacggg
     1081 gacgcctcct cccccccggc catatccatg ggttttcgcc gggcgctcca ccggcgagcg
     1141 ggacaccacc caccgcgagg atggcggcac agtgccaggc gacgagcgac agcacggtcc
     1201 gcggcagagg gactccgcgg acgagaaaga ggtgcgcagc cagccgcacc cgcacggtcc
     1261 ggccgacccg gtgacggcag ccccgggtcc catcgccgcc caagcgagcg cgacggagca
     1321 tccggccggg ggctctagcc tgctgctgcg tgtcgccgaa gcgcccctgg atgacgtgga
     1381 ccggggcgtc attcgcgccg acccgtacga tctcgcccgt atcggcgcgc gtcccggcga
     1441 cgtggtagtg atcgcgggag agcgccgcac cgccgcgatc gcgcacgcgc tgccgcctca
     1501 cctgcacggc acgcatcaga tcctgatgga cgggatggtc cgggagaatg cccgcgccgt
     1561 ggtcggtgag cgtgtggatg tttccgcgcc gcaggcggag ccggccagaa cggtgctggt
     1621 ggaatcactg gatggaggat ccttcgggcc gcgggagatc gccgacatcc gggagtggat
     1681 gtcgggacgc gcggtcatgt acggcgaccg gctcaaggtc acggcgttct ccaagcgcgg
     1741 gcacatgttc atcgtgcgcg gcaccgagcc ggaagcggtc gtggtggccg ggccgtacac
     1801 cgacgtccgc ctcaagaagg agcccgcgac cccgctcgtc gaaagcccgt cgctccgtgt
     1861 caaatacgag gacatcggtg gcctggacga ggagctgctg cgcgtccgtg agctcgtgga
     1921 actgccgctg aaatacccgt cgctattcgc ccggctgaag atcgagccac ccaaaggggt
     1981 gctgttgtat ggtccgcccg ggtcgggcaa gacgctgatc gcgcgcgctg tggcgagcga
     2041 ggtcaaggcc cacttcatac acgtcaacgg cccggagatc attcacaagt tctacgggga
     2101 aagcgaggca cggctgcggg aaatcttcga agaggcgcag cggcgtgccc cggcggttgt
     2161 tttcctcgac gaaatcgacg cgatagcgcc ccggaggcag gatgtctccg gagaggtgga
     2221 gaaacgcgtc gtcgcccagc tgctcgcgct catggacggc ctggttcccc ggggccaggt
     2281 ggtggtcatc ggcgcgacga acctgcccga ggcggtagat cccgcgctgc ggcgcccggg
     2341 aaggttcgac cgcgagatca cgatgaacgt gccaacccgc cccgggcggc tgcgcatcct
     2401 gaagatccat tcccgcggca tgccccttgc cgacgacgtc gatctggaag cgctcgccga
     2461 ggtcaccgcg ggctatgtcg gcgccgacct ggaggtgctg tgcaaggaag cgggcatgct
     2521 cgcgctgcgc gactgcctca ccgccgcggg aacgcaatcc gacaccgcag cggaccccga
     2581 actggccgcc gagcagacca agatcgatgc ccagcatttc cacgaggcac tcaagacgat
     2641 cgagcccaca gcgacccgtg agctggctct cgaacggccg aacgtccgct gggagcagat
     2701 cggcggcctg cgggatgtgc ggcagaccct gctgtctgcc ctggaactgc ctcatcagca
     2761 tcccgggcta ttcgcccagg ccgggatcaa gccgcccacg ggcttcctgt tcgaaggccc
     2821 ctccggcacc ggtaagtccc tcaccgtcac ggcgctcgcc gccgagacgg gcatgcggct
     2881 gatcagcgtc gatgccgcgt acctgttctc gaaatgggta ggcgagtcgg agaaggcgct
     2941 gcggacggtc ttcaagaagg ccaagcagtc cgcgccgtgc atcctgttct tcgatgacct
     3001 gcagacgttc gccccggcgc gccgcgcgct gagggacacc gacagcggtt tcgccgagcg
     3061 gctcgcccgg cagttcttcg ccgagcttga caagacgatc gggctcggcg acgtaatcgt
     3121 gatcggcgcc acgaaccgcc ccgacctggt agaccccgtc gcgcttcgcc ccgggcggtt
     3181 cgggttcgtg gtgcacttcc cgctgcccgg caccgggcag cgggaagaaa tcttccgggt
     3241 ccacctgcgg caggtccgcc tcgcaccgga cgtcgacgtc cgtgccctcg ccgaggagac
     3301 ccccggtttt tccggggcgg acatcgccgg cgtgtgcgaa cgggccctgc tgagcgagat
     3361 cgaggcgttc gtccggcgcc acggcgaccg ggccgacgag gaagccgcgc gccgcagcct
     3421 catcctgtcg gcgggcgcgc tccgcgaggc gctcgctcag gctcgcgctg cgctcccgcc
     3481 cactgcccag cctgaggagt aaaccatgcc gacctgcccc aaatgctcgc agccgatggc
     3541 acctgaccag gtcatcacca tcgccggcat ccgcgacaag gcggtgagct ggcaatgccc
     3601 tagcgaccac atcgaagccg tagccgtcca tccggtgcag atggcccatc tgcacattgg
     3661 ccaggaagag gaagacggcg gccggctcac ccggctcatc cacatggaca tggtcggcgc
     3721 ggagctgatc ctgttccgcg agattgtccc gctagcggga aagggcggcg agcaccatgg
     3781 ctgaccgctc agccctcccg ctggaccagg cacaatacgc tgcctatctg gcggagctgg
     3841 cagagattgt ccaccgcgat cctcagctgg agggcaccgc ggcactgcga gccttcgtgg
     3901 agacccacca ggaatggttc ggtcctcgca ccgccagaac aacgctgagc accgatgaca
     3961 caacgctgcg gacgctcatc cacatcatgg tgctcgccgc ctccgaactc gccgacctgc
     4021 accccgccag ccgcacatgg ctcaccgagc acggccgggg cctgccacca tgggacgtca
     4081 ccgttccccg cacggcgcaa cggctggtca ccttccgcag caaggtgtac ggggtggtcg
     4141 aatggcagcc ggaacccagg atcgaactcg ctcccagcct gaccgagcag gagcgtcgct
     4201 gggccaccgc gctagccatc gcgatcgggg aacgtcccca gtggtccagc cacgag
//
LOCUS       DASUBP010000058         3705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_655311, whole genome shotgun sequence.
ACCESSION   DASUBP010000058 DASUBP010000000
VERSION     DASUBP010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655311"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..185)
                     /locus_tag="VFU75_02480"
     CDS             complement(<1..185)
                     /locus_tag="VFU75_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465724.1"
                     /translation="MTAPKSSREGMASLRVERALRLLPDLEALTPLRALLVATSRADA
                     GAQWASSGPYLTLGKRAV"
     gene            complement(182..1576)
                     /locus_tag="VFU75_02485"
     CDS             complement(182..1576)
                     /locus_tag="VFU75_02485"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5465725.1"
                     /translation="MPTATKAPPLPGLSESPSAIRVLGVGLDGALMHTIRPWAAELQA
                     HIEFVADLPQAVRRLAAGRWDVVLTVLGEHPDEDLHWWANALRGRTGAPRLIAFANRP
                     SMGLALRAEQLGTHDLLALPVRREELMRSLRRVGRGTSEIAVPLESTANEDLGRYALV
                     GQSPAMLDVYKLIARVAPSTATVLIQGESGTGKEVVARAIHANGPKASGPFVALNCAA
                     IPENLLESELFGHEKGAFSGAVTRKIGRFELARGGTLFLDEIADMSLPLQAKILRAVQ
                     EREIERVGGGDPIPIDVRLIAASNRELRDAIQQGRFREDLYYRLAVVIVRLPRLSERG
                     SDLALLAAYFARQFASQYGKHITGFSDRTLELLTAHRWVGNVRELRNAIEQAVIVADG
                     GTILVEHLPDEIRGHEGSLAGTVSGELATLAEAEARHIARVLAHTGGQIGAAATILGI
                     HRNTLTRKIREYGL"
     gene            complement(1585..2193)
                     /locus_tag="VFU75_02490"
     CDS             complement(1585..2193)
                     /locus_tag="VFU75_02490"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU5465726.1"
                     /translation="MAAGPLLEWGVAARPLPGEHESGDHFLVKTFPEGAAIAVVDGLG
                     HGAEAAAAARRAVAAVEAAVRDPLLTLFRRCHDALVGSRGVVMSVASIDNRTSRMSWA
                     GVGNVDAWLLRPHAQDGKARTSLVTRGGVLGREVPPLTPISLPLDPGDLLVFATDGIR
                     EGFIEGMSLEDAPQRAASRIMATHGKGTDDALVLVAKYLGSA"
     gene            complement(2197..2607)
                     /locus_tag="VFU75_02495"
     CDS             complement(2197..2607)
                     /locus_tag="VFU75_02495"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma regulatory factor"
                     /protein_id="HEU5465727.1"
                     /translation="MPDAVICPIQADVDVVQTRQQGRNIAAEAGFSAGDQTVIAAAIS
                     EIARNILMYAKRGEIELRAVSFADRTGIVIIARDNGPGIRDIPRAMQDGYSTSGGLGL
                     GLPGAKRLMDEFEVVSSNGNGTTVTMKKWREVRA"
     gene            complement(2600..2971)
                     /locus_tag="VFU75_02500"
     CDS             complement(2600..2971)
                     /locus_tag="VFU75_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU5465728.1"
                     /translation="MEVPILKQGTYLIASIQSALTDADLSQLREALVARVGKFRARGV
                     IVDVTALDVMDSFAVRTLRDITHMARLRGAETVIVGIQPEVAFAMVQLGLTLKGVSTA
                     LDLEEGLAFLDAKARERIDRA"
     gene            complement(2995..>3705)
                     /locus_tag="VFU75_02505"
     CDS             complement(2995..>3705)
                     /locus_tag="VFU75_02505"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU5465729.1"
                     /translation="TSVYDNYVEALETGTFEALQAYARNLSERIIPRGVETHEVVGIV
                     LLLRDVLARSLFAKYHEDFTKLNRILDAYEPAANRIANTVAVGFVQERERVIRQQQEA
                     IRELSTPVLQVRERLLILPIIGVIDPQRARQLTEQLLRAIRTNRAKVVVIDITGVAAM
                     DANVANNLVQTVEAARLLGATVIVTGLSPEIAQTLVTIGVDLSEMATVGDLQGGIEEA
                     ERLIGYKVLPLSETSAAS"
BASE COUNT          574 a         1246 c         1301 g          584 t
ORIGIN      
        1 accgcccgct tgcccagggt caggtacgga ccggagctgg cccattgggc tcccgcgtcg
       61 gcccggctcg tcgccaccag cagggcgcgg aggggcgtca gggcctcgag atcggggagc
      121 aggcgaagcg cacgctccac ccgcagagac gccatgccct cgcgggagct cttgggagcg
      181 gtcacaaccc gtactcgcgg atcttgcggg tcaacgtgtt ccggtgaatc ccgaggatgg
      241 tggcggcggc gccgatctgc cctcccgtgt gcgcgaggac gcgcgcgatg tgccgcgcct
      301 cggcctccgc cagggtcgcg agctcacccg acaccgtacc cgcgagcgag ccctcgtgcc
      361 cgcggatctc gtcaggcagg tgctcgacca gaatggtccc gccgtcggcg acgatcaccg
      421 cctgctcgat ggcgttccgc agctcgcgga cattcccgac ccagcggtgc gccgtgagca
      481 gctcgagcgt gcggtccgag aagcccgtga tgtgcttgcc gtactgcgag gcgaactgac
      541 gcgcaaagta ggcggccagg agcgcgaggt cggagccgcg ttccgagaga cgggggaggc
      601 ggacgatgac caccgccagg cggtagtaca ggtcttcgcg gaagcggccc tgctgaatgg
      661 catcccgcag ctcacggttc gacgcggcga tgagacggac gtcgatcgga atgggatcgc
      721 cgccgccgac ccgctcgatt tcccgctcct gcacggcgcg gagaatcttg gcctggagcg
      781 gcaagctcat gtccgcgatc tcgtccagga acagggtccc gcctctggcg agctcgaagc
      841 ggccgatctt cctggtcacc gcgcccgaaa acgcgccctt ctcgtgaccg aagagctcgc
      901 tctcgaggag gttctccggg atcgcggcgc agttcagggc cacgaaggga ccggaagcct
      961 tcggaccatt ggcgtggatc gcccgcgcca cgacttcctt ccccgtcccg gattccccct
     1021 ggatgaggac ggtggccgtg ctgggcgcga cccgcgcgat gagcttgtag acgtcgagca
     1081 tcgcggggct ctgccccacg agcgcgtacc gtccgaggtc ctcgttcgcc gtcgattcga
     1141 gagggacggc gatctccgac gtgccccgcc ccacccggcg cagggaccgc atgagctcct
     1201 cgcggcgcac cggcagggcg agcaggtcat gggtgcccag ctgctccgcg cgcagtgcca
     1261 gacccatgga gggccggttc gcgaacgcga tgaggcgcgg cgccccggtg cgcccgcgga
     1321 gggcgttcgc ccaccagtgc agatcctcgt cggggtgttc gccgagcacg gtcagcacga
     1381 catcccagcg gcccgcggcc agccggcgga ccgcctgcgg cagatcggcg acgaattcga
     1441 tatgtgcctg caactcggcc gcccagggcc ggatggtgtg catcagcgcg ccgtcgagcc
     1501 ccactcccag gacgcgaatg gccgagggag actcggagag ccccgggagc ggaggagcct
     1561 tggtggcggt tggcatgggc ctgctcacgc gctccccagg tacttggcca cgaggaccag
     1621 agcgtcatcg gtccccttgc cgtgggtcgc catgatccgg gaggccgccc gctggggagc
     1681 gtcctcgagg gacatccctt cgatgaatcc ttcgcggatg ccatccgtgg cgaacaccag
     1741 gaggtctccg ggatcgaggg gaagactgat gggggtgagg ggcgggactt cccggcccag
     1801 aacgccgccc cgcgtcacga gactggtgcg cgccttccca tcttgagcgt gggggcgcag
     1861 cagccaggca tccacgtttc cgacccccgc ccaactcata cgggacgtgc ggttgtcgat
     1921 cgacgcgacg ctcatcacca caccacggct ccccacgagc gcgtcgtggc agcgccggaa
     1981 gagcgtgagg agtgggtccc gaacggcggc ctcgacggcg gcgacggccc gtcgggcggc
     2041 cgcggcggct tcggctccgt gccccagacc atcaaccacg gcgatggccg cgccttcggg
     2101 gaaggtcttg acgaggaagt ggtccccgga ctcgtgctct ccggggagcg gccgcgcggc
     2161 gacgccccat tcgaggagtg ggccggcggc catccgttac gcgcggacct cacgccactt
     2221 cttcatggtt acggtggtcc cgttgccgtt gctggagacc acctcgaact cgtccatgag
     2281 gcgcttggcg ccgggaaggc cgagtcccag accgccggag gtcgagtacc cgtcctgcat
     2341 ggcacgaggg atatcccgga tgccggggcc gttgtcacgg gcaatgatga cgatcccggt
     2401 tcggtccgcg aaggagacgg cgcgaagctc gatctcgccc cgcttggcgt acatgaggat
     2461 gttgcgggcg atctcggaga tcgccgcggc gatcacggtc tgatccccgg cggagaatcc
     2521 cgcctcggcg gcgatgttcc gtccctgctg gcgggtttga acgacgtcga cgtcggcctg
     2581 aatggggcag atgacggcgt caggcacggt cgatacgctc ccgggccttg gcatccagga
     2641 atgccaggcc ctcctcgagg tcgagcgcgg tcgagacgcc tttgagcgtg agccccagct
     2701 ggaccatcgc aaaggcgact tcgggctgaa tgccgacgat gaccgtttcc gcgccgcgga
     2761 gccgcgccat gtgcgtgatg tcgcgcagcg tccggacggc gaacgagtcc atgacgtcga
     2821 gcgccgtcac gtccacgatg acgccgcggg cgcggaattt tcccacgcgc gccaccagcg
     2881 cctcacgcag ctgcgacaga tcggcgtcgg tcagcgcgga ctggatcgac gcgatcagat
     2941 aggtgccctg cttcagaatt gggacttcca caggtccttt tctttcggct caggtcagga
     3001 cgcggccgac gtctccgaca acgggagcac cttgtaaccg atgagccgtt cggcttcttc
     3061 aatgccgcct tgcaggtcac ccaccgtggc catctcggag aggtcgacgc cgatggtgac
     3121 cagcgtctgc gcgatctcgg gcgacaggcc cgtgacgatg acggtggcgc ccagcaggcg
     3181 ggccgcctcg acggtctgga ccaggttgtt cgccacgttg gcatccatcg ccgcgacgcc
     3241 cgtgatgtcg atgacgacga ccttcgcacg gttggtccgg atggcgcgca gcagctgttc
     3301 cgtcagctgg cgcgcgcgct gcgggtcgat gacgccgatg atcgggagaa tgaggagccg
     3361 ttccctgacc tggaggaccg gcgtggacag ctcgcggatc gcttcctgtt gctgccggat
     3421 gacgcgctcg cgctcttgca cgaagccgac cgccaccgtg ttcgcgatcc ggttcgcggc
     3481 cggttcgtac gcgtcgagaa tccggttcag cttggtgaag tcttcgtggt acttggcgaa
     3541 gagcgagcgg gccagcacgt cgcggaggag gagcacgata ccgacgactt cgtgcgtttc
     3601 aacgcctcgc ggaatgatgc gctcggacag gttgcgcgcg taggcctgga gggcctcgaa
     3661 cgtcccggtc tcgagcgctt ccacgtagtt gtcgtagacc gacgt
//
LOCUS       DASUBP010000059         2785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1295740, whole genome shotgun sequence.
ACCESSION   DASUBP010000059 DASUBP010000000
VERSION     DASUBP010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295740"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VFU75_02510"
     CDS             complement(<1..891)
                     /locus_tag="VFU75_02510"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU5465730.1"
                     /translation="MPPLQRALRSFDATMIVIGSMIGSGIFITSAESARLVGAPGWLL
                     VAWGLAGILTITGALSCAELAAAFPRAGGQYVFLRDAYGPGVGFLFGWALFTVIQSGT
                     IAAVAVAFASFLGVLLPGISPVRFLVPPIALGGSGFAVSLSTEQLTALLSILLLTFAN
                     ARGLKQGVRVQNISTVAKIAALAGLVVIGLAFGDHAAGAARTSSWWSPAANGAAQVGT
                     FALLMLVGRAMVGPLFAQSAWNNVTFAGAEIENPGRNLPRALLLGCATVVGLYLLANL
                     AYVTVLPLDGIRHAPNNRVAV"
     gene            977..>2785
                     /locus_tag="VFU75_02515"
     CDS             977..>2785
                     /locus_tag="VFU75_02515"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEU5465731.1"
                     /translation="MIRLFRCAALLAFASAAGNLQAQARSPLPEVTAPLKVIDTAFMN
                     RHANACTDFFAFANGGWIAKDTIPAAYSSSGVARDMSDRNELAVRSVLEDAMAKRGKA
                     PAGSTPRKLGTYYATCMDSTAADAAGITPIKPWLAAIDSIQSPTQLLPAIAALQKHGA
                     NVGFYYFPSADLHDASHYIVDLSQGGLGLPDRDYYTDSGSAADSTRQAFVGHIAKLFE
                     LAGENAPQAGSEAAEVLALETEMAKASLTRVALRDPAASDHSTTAEQLAGMTPGLDWP
                     GYFTAIGIQAPVQRLNVGEPDYFKRLSRLIDSVPLSTWRAYLRYHAIGSVASWLSTPF
                     VQENFAFSSRFSGARALLPRWKRCLRETDGDLGEALGQAYVAKTFSPEARRRAKTVID
                     DIRAAFRERLLHLTWMSEATRAEALKKLSLMGEKVGYPEKWRDYSKLDVQEGSFALNV
                     ARANVFEWDRTVNRPGQPVDTTEWGITVPTVNAYYDGSKNEMVFPAGALTPQTFDAKA
                     DDAGNYGSLGGSWAGHELTHGFDDEGRHYDERGNLRDWWQPADSATFTKEADKIVAQF
                     SGYLQVDTIHGNGKLTEGENIADLGGVLTGYDALERA"
BASE COUNT          500 a          909 c          965 g          411 t
ORIGIN      
        1 aacggcgacc cggttgttgg gcgcgtgccg aatgccgtcg aggggaagga ccgtgacgta
       61 cgcgaggttg gcgaggaggt agagtcccac cacggtggcg cagccgagca gcagggcgcg
      121 aggcaggttg cgcccgggat tctcgatctc cgccccggcg aacgtgacgt tattccaggc
      181 cgattgcgcg aagagtggcc ccaccatcgc gcggcccacg agcatgagga gggcgaaagt
      241 gcccacttgg gctgcgccat tcgcggcggg actccaccag gacgacgtgc gggccgcacc
      301 cgccgcatga tctccaaacg ccaggccgat gacgaccagt cccgcgagtg ccgcgatctt
      361 cgccacggtc gagatgttct gcacccgcac gccctgcttc agcccccggg cgttggcgaa
      421 ggtgagcagg aggatggaga ggagggccgt gagctgttcc gtggacaggc tgacggcgaa
      481 ccctgatccg ccgagagcga tcggcggcac gaggaatcga accggggaga tgccggggag
      541 aaggacaccc agaaagctcg caaacgcgac ggcgaccgcc gcgatggtgc cgctctgaat
      601 gacggtgaag agggcccagc cgaagagaaa ccccaccccc gggccgtagg catctcggag
      661 gaagacgtac tgtccgcccg cccgggggaa ggcggccgcc aactcggcgc agctgagcgc
      721 gccggtgatg gtcaggatcc ccgccagccc ccaagccacc aggagccacc cgggggcccc
      781 gacgaggcgc gccgactcgg cggaggtgat gaagatgccc gacccgatca tcgagccgat
      841 cacgatcatg gtggcatcga aagaacggag cgcgcgctgg aggggcggca tggccgtgta
      901 aggttgacac ctgttgtcgg gatgggctag cctacctcgc cccgccgtca caattcacga
      961 atcgctcgga gtgtccatga tccggttgtt ccgttgcgcc gcgctgctcg cgtttgcaag
     1021 cgccgccggc aatcttcagg cgcaggcccg ttcgccgctc cccgaggtca ccgcgccgct
     1081 caaggtgatc gacacggcgt tcatgaatcg ccacgcgaac gcctgcacgg atttcttcgc
     1141 gtttgcgaat ggcggctgga tcgcgaagga caccattccc gcggcgtact cctcgtcggg
     1201 cgtggcgcgc gacatgtcgg accgcaacga gctggcggtg cggtcggtgc tggaagacgc
     1261 gatggcgaag cgcggcaagg cgccggcggg gagcactccc cggaagctcg gcacctacta
     1321 cgccacgtgc atggactcga cggcggcgga cgctgccggc atcacgccga tcaaaccctg
     1381 gctggcggcg atcgattcca tccagagtcc cacgcagctg ctcccggcga tcgcggccct
     1441 gcagaagcat ggcgccaacg tcgggttcta ttatttcccc agtgccgacc ttcacgacgc
     1501 gtcgcactac atcgtggacc tcagccaggg tggcctgggt ctgcccgacc gcgactacta
     1561 cacggactca ggatcggcgg cggactcgac ccggcaggcg ttcgtgggac acatcgccaa
     1621 gctgttcgag ctcgccggcg aaaatgcgcc gcaggccggg agcgaggccg cagaagtgct
     1681 cgcgctcgag accgaaatgg ccaaggcctc cctcacgcgc gtggcgctgc gggatcccgc
     1741 cgcgagcgat cactccacga cggccgagca gctggccggc atgactccgg ggctcgattg
     1801 gccgggttac ttcaccgcca tcggcatcca ggcgccggtg cagcgcctca acgtggggga
     1861 gcccgactac ttcaagcggc tctcccggct catcgattcg gtcccgctct ccacctggcg
     1921 ggcctacttg cgctatcacg ccatcggcag cgtcgcgagc tggctctcga cccccttcgt
     1981 gcaggagaac ttcgcattct cctcacggtt ctcgggcgcg cgcgctctcc tgccgcgatg
     2041 gaagcgttgt ctgcgggaaa ccgacggtga tctgggggag gcgctagggc aggcctacgt
     2101 ggcgaagact ttttcgccgg aggcccggcg gcgggcgaag accgtcattg acgacatccg
     2161 cgccgccttc cgggagcggt tactgcacct gacctggatg tcggaggcca cgcgtgccga
     2221 agcgctgaag aagctgtcgt tgatgggaga aaaggtcggc tatcccgaga agtggcgcga
     2281 ctattcgaag ctcgacgtgc aggagggctc gttcgccctg aatgtcgccc gcgcgaacgt
     2341 cttcgaatgg gatcggaccg tgaaccggcc ggggcagccg gtggacacca cggagtgggg
     2401 catcacggtc ccgaccgtca atgcgtacta cgacgggtcc aagaacgaga tggtcttccc
     2461 ggccggcgcg ttgaccccgc agaccttcga tgcgaaggcc gacgacgcgg ggaactacgg
     2521 ctccttgggt ggcagctggg cagggcacga gctgacgcac ggcttcgatg acgagggccg
     2581 gcattacgac gagcgcggca acctgcggga ttggtggcag cccgccgact cggcgacgtt
     2641 caccaaggaa gccgacaaga tcgtggcgca gttctccgga tatctccagg tggacacgat
     2701 tcacggcaac ggcaagctga cggaaggcga gaacatcgcc gatctcggcg gggtcctgac
     2761 gggttacgac gcgctggaac gcgcg
//
LOCUS       DASUBP010000060         6247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_805446, whole genome shotgun sequence.
ACCESSION   DASUBP010000060 DASUBP010000000
VERSION     DASUBP010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6247
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_805446"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1148)
                     /locus_tag="VFU75_02520"
     CDS             complement(<1..1148)
                     /locus_tag="VFU75_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5465732.1"
                     /translation="MTGRRLGIGFIGSGFNARFHMLGFQAVRDADIRGVWSPNAGRAA
                     EATAYAKKLDVGDAKPYKSIADMVADPAIDALWLCGPNQARIDNVEEIVHAMTSGKGE
                     LKGLACEKPLARNVAEAEKVVELVKKAKLKHGYLENQRFAPQVETGKNLIWARGAKLT
                     GRPYLARAAEEHSGPHMPWFWQGKLQGGGVLNDMMCHSALVVRHLLTAPGAPASSVKP
                     VRITGHIASLKWSRPEYTKKLADTMGKDVDYAKTPAEDFASVMIEFQAADGHKVLGEA
                     TTSWSFVGAGLRLSAELLGPEYSMSWNTLDSGLKLFFSREVQGKSGEDLVEKQNAEIG
                     QMPVVASEAAAYGYEAEDRHFVRVFLGKEEPLLTFDDGLEVVQMLMTA"
     gene            complement(1145..1738)
                     /locus_tag="VFU75_02525"
     CDS             complement(1145..1738)
                     /locus_tag="VFU75_02525"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025004.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HEU5465733.1"
                     /translation="MSMNRRDAAKALAVAPVAAAFRWAPESVREAAALAREALDRQAP
                     FKPKFFTAHEWRSVRVLVDLIIPADEQSGSATQAGVPEFIDFMCIDDQSLQTPVRGGL
                     AWMDTASHESFGKAFVDGTAADQTGLLDRIAWPRKAKPEDAQGAAFFTSFRDLTASGF
                     YSSRMGVHDLKYIGNTFVPEWKGCPPEALAKLGVRYS"
     gene            complement(1738..3423)
                     /locus_tag="VFU75_02530"
     CDS             complement(1738..3423)
                     /locus_tag="VFU75_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEU5465734.1"
                     /translation="MQRDETYDVCIVGSGAGGGMAAYALTRAGARVIVLEAGGPWDNT
                     KDSAMLKWPYETPRRGAGTHQRPFGEFDACIGGWNIDGEPYTVAPGNKFSWWRARMVG
                     GRTNHWGRISLRFGPYDFQHKSMDGLGDDWPITYDEVAPYYDQVDRLVGVFGSREGLP
                     NEPDGLFLPPPKPRCYELMVKDAADRLHVTCIPSRLSILTQSINGRAHCHYCGQCSRG
                     CSVNANFSSTNVLIAPALRTGKLRLVTHAMAREVTMGKDGRANGVSYIDKNTGTERHV
                     SAKVVVLAASACETSRLLLNSRSSLFPSGLGNEGGKVGKYLTDTTGTDVSGYIPKMED
                     HVPHNHDGVGGMHVYMPWWLDNKTLDFPRGYHIEVWGGTSIPTNGLLSGIESYPGGGG
                     YGKQLKADYRKYYGAYVGFSGRGEMIPNEDSYCELDPGTVDQWGIPVLRFHWKWTDHE
                     YLQCKHMQETFRSLVDEMGGTVFDPMPTKEQGYGLSTGGSIIHELGGARMGEDPGTSV
                     VDPHCQVHGVKNVFVADGAPFVSQADKNPTWTILALSLRTSEYVADQMKKGAL"
     gene            3492..5489
                     /locus_tag="VFU75_02535"
     CDS             3492..5489
                     /locus_tag="VFU75_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin"
                     /protein_id="HEU5465735.1"
                     /translation="MTSWTRREFVQQAGFIAGGLVTARTARAAGSLRYRVDAADITLG
                     NDAVNATWHLSGGVLKGLTITDIGSHRTLPLSEEVVTLVLQDGTAIAASELQARAPTY
                     ERLPGDHRASRFADRLPGHQVTVELTDPASRLAISWRAILRTDSRYLRQEVTIRAVGA
                     DLPVKEIRLGELTLPGARISGSVDGSPVVTDGWFLGFEHPLSHHTIENDRVRYGLSRE
                     LPIRPGAPVTCSSVVGPVTPGQLRRDFLLYVERERAHPYRPFLHYNSWYDLGYFSKYD
                     EAGALAVIDAFGVELHRKRAVVLDSFLFDDGWDDPETLWHFHSGFPHGFTPIRAAAAK
                     YGAAPGVWMSPWGGYGKPRDERVAYGKTQGFETNEGGFELSGPKYYQRFRDVCFDMIK
                     TYGVNQFKFDGTGNVSHVFPGSLFDSDFDAMLSLIGDLRNEKPDLYVNLTTGTYPSPF
                     FLRSCDSIWRGGDDHEFAGVGSWRQKWITYRDADTYAGIVQKGPLFPLNSLMLHGMIY
                     AKHAHNLDTDPNGDFQSEVRAYFGTGTQLQEMYITPGLLTPANWDALGESARWSRSRA
                     AALSDTHWIGGDPAQLEPYGHAAWTPGRGILCLRNPSDKPQSFPLNIGNALELPDHAP
                     QRFMLRRAYRSERAQPPIEAGVDKAVEIPLAPFEVLTLETV"
     gene            complement(5611..>6247)
                     /locus_tag="VFU75_02540"
     CDS             complement(5611..>6247)
                     /locus_tag="VFU75_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465736.1"
                     /translation="AQSPAFGAPEASPDITGGPGFEGLGELQKFVDAGGEIVTLASST
                     SMISGTGIAAALSPHGAGGLFHPGSVVRARIRHAASPILYGYPSQFTIFRGNGPLFEV
                     DRRDSAMLVLQYGAGIKGEKEEGPMLGMPETAKAPAAKEKEKSAADSSYVASGMVRNE
                     NEILGQGAIFDVPVGKGRVIAFTFDALHRYLNHHEFPLVWNALLNWDARIR"
BASE COUNT         1022 a         2149 c         1966 g         1110 t
ORIGIN      
        1 tacgccgtca tcagcatctg caccacttcg agcccgtcat cgaacgtcag caggggctct
       61 tccttcccca gaaacacccg aacgaagtgg cgatcctcgg cctcgtaccc gtacgccgcc
      121 gcctcactcg ccaccaccgg catctgcccg atctcggcat tctgcttctc gaccaggtct
      181 tcgccgctct ttccctgcac ttcccggctg aagaaaagct tgagcccgga gtcgagcgtg
      241 ttccacgaca tcgagtactc ggggccgagc agctcggccg agagacgcaa gcccgcgccg
      301 acgaagctcc aggaggtggt cgcctcgccc agcaccttgt gcccatcggc ggcctggaac
      361 tcgatcatca cgctcgcgaa gtcctcggcc ggtgtcttcg cgtagtcgac gtccttcccc
      421 atggtgtcgg ccagcttctt cgtgtattcg ggccggctcc acttgaggct cgcgatgtgc
      481 ccggtgatcc gcacgggctt cacggaagag gcgggagcac ccggggccgt gagcaggtgc
      541 cggacgacga gcgccgaatg gcacatcatg tcgttgagca cgccgccgcc ctggagcttg
      601 ccctgccaga accagggcat gtgcggtccc gagtgctcct ccgcggcccg cgccaggtag
      661 ggccggccgg tgagcttcgc cccgcgcgcc caaatcagat tcttgccggt ctcgacctgc
      721 ggtgcgaagc gctggttctc gagatacccg tgcttgagct tcgccttctt caccagctcc
      781 acgaccttct cggcctcggc cacgttccgc gcgagcggct tctcgcaggc gagccccttc
      841 agctcgccct ttccgctcgt catcgcatgg acgatttctt cgacgttgtc gatccgtgcc
      901 tggttcgggc cgcagagcca gagtgcgtcg atcgcgggat ccgccaccat gtcggcgatc
      961 gacttgtagg gcttcgcgtc cccgacgtcg agcttcttcg cataggccgt ggcctcggcg
     1021 gctcggcccg cattcgggct ccaaacgccg cggatatcgg cgtcacgaac cgcctggaag
     1081 ccgagcatgt gaaagcgggc gttgaatccc gagccgatga atccgatccc caatctgcgg
     1141 ccggtcatga gtagcggact cccaatttgg ccagcgcctc aggagggcag cccttccact
     1201 cgggcacgaa cgtgttgccg atgtacttga gatcgtgcac acccatgcgg ctggagtaga
     1261 acccggaggc cgtgaggtcg cggaagctcg tgaagaatgc cgctccctgc gcgtcctccg
     1321 gcttggcctt ccggggccac gcgatgcggt cgaggagacc cgtctgatcg gccgccgtgc
     1381 cgtcgacgaa cgccttcccg aaggattcgt gcgacgcggt gtccatccac gcgagcccgc
     1441 cgcgcacagg agtctggagg ctctggtcgt cgatgcacat gaagtcgatg aactccggca
     1501 cgccggcctg cgtcgcgctc cctgactgct catcggcggg gatgatcagg tccaccagca
     1561 cgcgcaccga ccgccattcg tgcgccgtga agaacttcgg cttgaagggc gcctgccggt
     1621 cgagcgcctc ccgggccagg gcggccgcct cacgcaccga ttcgggtgcc cagcggaacg
     1681 ccgccgccac gggagccacc gccagggctt tcgccgcgtc gcggcgattc atgctcatca
     1741 gagcgcccct ttcttcatct ggtccgcgac gtattccgac gtgcgaagcg agagcgccag
     1801 aatggtccag gtagggttct tgtcggcctg actgacgaac ggtgcgccat ccgcgacgaa
     1861 tacgttcttg acgccgtgga cctgacagtg gggatccacc acggacgtgc ccggatcctc
     1921 ccccatgcgc gccccaccca attcgtggat gatgctgccg ccggtggaaa gcccgtatcc
     1981 ctgttccttc gtaggcatcg ggtcgaacac ggtcccgccc atctcatcca ccagcgagcg
     2041 gaacgtctcc tgcatgtgct tgcattggag atactcgtga tcggtccact tccagtggaa
     2101 gcgcaggacc ggaatgcccc actgatcgac ggtgccgggg tcgagctcgc agtaactgtc
     2161 ctcgttcggg atcatttccc cccgccccga gaacccgacg tacgcgccgt agtacttgcg
     2221 gtagtcggcc ttgagctgct tcccgtatcc gccgccaccc ggatacgact cgatgccgct
     2281 caggagcccg ttggtcggga tcgacgtgcc accccagact tcgatgtggt agccgcgcgg
     2341 gaaatcgaga gtcttgttgt cgagccacca gggcatgtac acatgcatcc cgccgacgcc
     2401 gtcatgattg tgggggacgt ggtcttccat cttcgggatg taaccggaga cgtcggtccc
     2461 cgtggtgtcc gtcaggtact tcccgacctt cccgccttcg ttgccgagcc ccgacggaaa
     2521 gagtgacgag cgcgaattga gcagcaggcg agaggtctcg cacgcgctgg cggccagcac
     2581 cactaccttc gcgctgacgt gccgttcagt ccccgtgttc ttgtcgatat agctcacgcc
     2641 attcgcgcgg ccgtccttcc ccatcgtgac ctcgcgcgcc atcgcgtgag tcaccagcct
     2701 gagcttgcca gtcctgagcg cgggagcgat caggacgttc gtggacgaga aattcgcgtt
     2761 caccgagcac ccgcggctgc attgtccgca gtagtggcaa tgcgcgcgtc cgttgatcga
     2821 ctgggtcaga atcgagaggc gtgagggaat gcaggtgacg tggaggcggt ccgccgcatc
     2881 cttcaccatc agctcgtaac agcgcggctt gggaggtggc agaaagaggc cgtcgggctc
     2941 gttcgggagc ccttcgcggc tgccgaacac gccgaccagc cggtcgacct ggtcgtagta
     3001 cggcgcgacc tcgtcatagg tgatgggcca gtcgtcgccg agcccatcca tggacttgtg
     3061 ctggaagtca tagggaccga agcggaggga gatgcggccc cagtgattgg tacgaccacc
     3121 caccatgcgg gcccgccacc aggagaactt gttgccgggc gccacggtgt agggctcgcc
     3181 gtcgatgttc cagccaccga tgcaggcgtc gaattctcca aagggccgct gatgggtccc
     3241 ggcaccacgg cgcggcgtct cgtacggcca cttgagcatg gccgagtctt tcgtgttgtc
     3301 ccagggaccg ccggcttcga gcacgatgac gcgcgcaccc gcgcgcgtga gcgcgtaggc
     3361 cgccattccg ccccccgccc cggagcccac gatgcacacg tcgtacgttt cgtcacgctg
     3421 catccgacac cccaagcaag ttgacgcccg aagctagcgc ggaatcagcg gaggtggcaa
     3481 cgttcccgcc catgacttcc tggacacgtc gcgagttcgt gcagcaggcg gggttcatcg
     3541 ccggcgggtt ggtcacggcc cggacggccc gggcggccgg ctcgctgcgc taccgcgtcg
     3601 acgccgccga cattaccctc ggcaatgacg ccgtcaacgc cacctggcat ctctccggcg
     3661 gcgtcctcaa agggctcacg atcaccgata tcggttccca ccggaccctg cccttgtcgg
     3721 aggaagtggt cacgctcgtg ctccaggatg ggacggcgat cgcggcgtcg gagctacagg
     3781 cccgggcccc gacgtacgag cgcttgcccg gagatcaccg cgcctcccgg tttgcggacc
     3841 ggctcccggg ccaccaggtc acggtcgagc tcaccgatcc cgcgtcgcgc ctggcgatct
     3901 cctggcgcgc cattctccgg accgattcgc gttacctgag gcaggaggtc acgattcgcg
     3961 ccgtcggcgc cgacctcccc gtgaaggaaa tccggctcgg cgagctgacc cttcccgggg
     4021 cccggatctc cggctccgtg gacggatccc ccgtcgtgac ggacggctgg ttcctgggct
     4081 tcgagcatcc cttgtcgcat cacaccatcg agaatgaccg ggtgcggtac gggttgagcc
     4141 gggagctgcc gatcaggccg ggggcgcctg tgacgtgctc ctccgtggtc gggcccgtga
     4201 cgccgggaca gttgcggcgg gacttcctgc tctacgtgga gcgcgagcgc gcgcatccct
     4261 atcgcccgtt cctgcattac aactcctggt acgacctggg ctacttctcg aagtacgacg
     4321 aggcgggtgc cctcgcggtc atcgacgcgt tcggggtgga gcttcaccgg aagcgcgcgg
     4381 tggtcctcga ctctttcctg ttcgacgacg ggtgggacga tcccgaaacg ctctggcact
     4441 ttcactcagg gttcccgcac ggcttcaccc ccatccgcgc ggccgcggcg aagtacgggg
     4501 ccgcgcccgg ggtgtggatg tctccctggg gcggctacgg caagccccgt gacgagcgcg
     4561 tggcatacgg caagacgcag gggttcgaga ccaacgaagg cgggttcgag ttgtcggggc
     4621 cgaagtatta ccagcggttc cgcgacgtct gcttcgacat gatcaagacc tacggcgtca
     4681 atcagttcaa gttcgatggg acgggaaacg tctcacacgt atttcccgga tccctgttcg
     4741 actccgactt cgacgccatg ctctccctga tcggggacct ccggaacgag aagcccgatc
     4801 tctacgtcaa tctcacgacc gggacatacc cgtcgccgtt cttcctgcgt tcctgcgact
     4861 cgatctggcg cggaggtgac gatcacgagt tcgccggggt cggcagctgg cggcagaagt
     4921 ggatcaccta ccgggatgcc gacacctacg ccggcatcgt gcagaagggt cccctgttcc
     4981 cgctcaactc cctgatgctg cacggcatga tctacgccaa gcacgcccac aatctggaca
     5041 cggatccgaa cggggatttc cagtcggagg tgcgggcgta tttcggaacg gggacgcagc
     5101 tccaggagat gtacatcacg ccgggattgc tgacgcccgc gaattgggac gcactgggcg
     5161 agtcggcgcg ctggtcgcgt tcgcgcgctg ccgcgctgtc cgacacgcac tggatcggcg
     5221 gcgatcccgc gcagctcgag ccgtatggcc acgcggcctg gacgccggga cgcgggattc
     5281 tctgcctccg caacccctcc gacaagccgc agtcgttccc actcaacatc gggaacgcgc
     5341 tcgagcttcc cgatcatgcg ccgcaacggt tcatgctgcg ccgagcctac cggagcgagc
     5401 gggcgcaacc tcccatcgag gccggcgtgg acaaggccgt cgagatcccg ctcgcgccgt
     5461 tcgaagtgct tactctggag acggtctagc gcagcgaatc cctgtcgtcc ccaggccaag
     5521 gcctgggccc gacgcaacat cacgcctagg cgtggtgcat ggtcgggccc actcttacag
     5581 agtgggaacg aaccaagatg ccgacctccc tcatcgtatc cgggcatccc agttcaacag
     5641 cgcattccac accagcggga actcgtggtg gttcaggtac cggtgcaatg cgtcgaaggt
     5701 gaacgcgatc acccgtccct tcccgaccgg cacatcgaag atcgcgccct gacccagaat
     5761 ctcgttctcg ttccgaacca ttcctgacgc cacatacgag ctgtcggccg cggatttctc
     5821 cttttccttc gccgcgggtg ccttcgcggt ctccggcatg ccgagcatcg gtccttcctc
     5881 cttctcgccc ttgatgcccg ccccgtactg aagcacgagc atcgcgctgt cgcggcggtc
     5941 cacctcgaag aggggcccat tgccgcggaa gatcgtaaac tggctcgggt acccgtagag
     6001 aatgggcgac gccgcgtgac ggatccgggc acgcacgacg gaaccggggt ggaagaggcc
     6061 gcccgcgcca tggggagaaa gggcggcggc gatgccggtg cccgagatca tcgaggtcga
     6121 agaggcgagc gtcacgatct ccccaccagc gtcgacgaac ttctgcagct cgccgagacc
     6181 ttcgaatccg ggaccgcccg tgatgtcggg cgacgcctcg ggcgcaccaa atgccggact
     6241 ctgcgcc
//
LOCUS       DASUBP010000061         2353 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1023209, whole genome shotgun sequence.
ACCESSION   DASUBP010000061 DASUBP010000000
VERSION     DASUBP010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1023209"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2133
                     /locus_tag="VFU75_02545"
     CDS             <1..2133
                     /locus_tag="VFU75_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU5465737.1"
                     /translation="GDTLVVFPATPAGFGDTLVFTVTYRGKIKNGSGLTFITTDGMPH
                     RPRQIWSQGEDHDNHFWFPTYDFPNDKMTWELAATVDRNDMAVSNGRFVSDVKQGATH
                     TLTWNQDRPSATYLVSLVVAPLAKIHDTWEGIPVDYYTYHDDSSRAWRLFHETPDMIG
                     VYSQLTGVKYPWAKYAQTTVADFFGGMENVSATTLVDWLPDAKAYADRPWYSYILIPH
                     ELAHQWFGDYVTTNNWANMWLNEGFAEFMPGQYWGTRQGPHAEQDYYLDEYRQYLQIE
                     GNRSMPLASMGSNNIYPKGALVLEMLKHYLGEKRFWASIHHYLTTHAMSGATSDDLRQ
                     AFLAETGENLDWFWSEWVYQAGYPQITVTAAYDSAGHTLALDIKQTQQDSLKADSTGL
                     KFTVPQVFKMPVTIRVGTAKGDVTQQVWLDSREQKVTIPVSGAPTMVIFDDGNDVLKR
                     LDFDQPTAWLATQLTQDADLWDRAWVIEALARRPADAAAGAALANAAQHADYFLTRQQ
                     AAEALDTFPAAVASPALTAALKDTSAAVRAAAIRSLAHVGGADVATTARNLFASDQSY
                     DVRAAALQALVSADPAGAHATIAQGLSTPSYRDAIQSAALRAVFGANDTSFIAKVDSM
                     AADQPIPVHLLAALGTKGNTHALDLLAGHLNDPRRTVRQWTLQAYHFTLARQDKALAV
                     TQLKAAVDGIRYADTKKAAEDLLKALQQ"
     gene            complement(2134..>2353)
                     /locus_tag="VFU75_02550"
     CDS             complement(2134..>2353)
                     /locus_tag="VFU75_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465738.1"
                     /translation="RAELGFGIPGARLALFSDAGWAGVGRFAFTAQPLISAGLGASFL
                     DGLLRVDLARALAVQRGWRLELYTDAAL"
BASE COUNT          435 a          812 c          749 g          357 t
ORIGIN      
        1 ggcgacaccc tggtggtctt cccggccacg ccggccgggt tcggggacac cctggtgttc
       61 acggtgacgt atcgcgggaa gatcaagaac ggctcggggc tgacgttcat cacgacggac
      121 ggcatgccgc atcggccgcg gcagatctgg agccagggcg aagaccacga caatcacttc
      181 tggttcccca cgtacgactt tccgaacgac aagatgacct gggagcttgc cgccaccgtc
      241 gatcggaacg acatggccgt gtcgaatggc cggttcgtgt cggatgtgaa gcagggggcg
      301 acccacacgc tcacgtggaa tcaggaccgc ccgtccgcca cgtatctcgt gtccctcgtg
      361 gtcgcgccac tcgccaagat ccacgacacc tgggaaggca tccccgtcga ttactacacg
      421 tatcacgacg acagctcgag ggcctggcgg ctctttcacg agacgcccga catgatcggt
      481 gtctactcac agctcaccgg cgtgaagtac ccctgggcca agtacgcgca gaccacggtg
      541 gcggacttct tcggcggcat ggagaacgtc agcgcgacca ccctggtcga ctggctcccc
      601 gatgccaagg cgtacgccga ccgcccctgg tactcgtaca tcctgattcc gcacgagctg
      661 gcccatcagt ggttcggcga ctacgtgacg acgaacaact gggccaacat gtggctgaac
      721 gagggattcg cggagttcat gcccggccag tactggggga cccgccaggg tccgcacgcc
      781 gagcaggact actatctcga tgagtaccgg caatacttgc agatcgaggg gaatcgctcc
      841 atgccgctcg cgtcgatggg atcgaacaac atctatccca agggcgcgct ggtcctcgag
      901 atgttgaagc actatctcgg tgagaagcgg ttctgggcgt ccatccacca ttacctcacg
      961 acgcacgcga tgagcggcgc gacgtcggat gatctccgtc aggcgtttct cgcggagacc
     1021 ggtgaaaacc tggactggtt ctggagcgag tgggtctacc aggcggggta tccgcagatc
     1081 acggtgaccg cggcgtacga cagcgcgggg cacacgctcg ccctcgatat caagcagacc
     1141 cagcaggact cgctcaaggc cgacagtacc ggcctcaaat tcaccgttcc gcaggtcttc
     1201 aagatgccgg tgaccattcg cgtcgggacg gcgaagggcg acgtgacgca gcaggtctgg
     1261 ctcgactcgc gggagcagaa ggtgacgatt cccgtgagcg gcgcgccgac gatggtgatt
     1321 ttcgacgacg gtaacgacgt cttgaagcgg ctcgacttcg accagcccac cgcctggctc
     1381 gcgactcagc tcacgcaaga cgccgatctc tgggaccgcg cctgggtgat cgaggccctc
     1441 gcccggcgcc ccgcggatgc ggccgccggc gccgccctcg cgaacgccgc ccagcacgcc
     1501 gactacttcc tcacccgtca gcaggcggcc gaggcgctcg acaccttccc cgccgcggtg
     1561 gcgtcaccgg cgctgaccgc ggctctgaag gacacctcgg cggccgttcg ggcggccgcg
     1621 atccgttcgc tcgcccacgt tggcggcgcc gatgtggcga cgacggcgcg caacctcttc
     1681 gcgtcggacc agagctatga cgtacgcgcg gccgcactcc aggccctcgt gagcgccgac
     1741 cccgccggtg cccatgccac catcgcgcag ggcctgtcca caccctcgta ccgggacgcg
     1801 atccagagtg ccgcgctgcg cgccgttttc ggggcgaacg acacgtcgtt catcgcgaag
     1861 gtcgactcga tggccgccga ccagcccatt cccgtgcacc tcctggcggc cctcgggacc
     1921 aaggggaaca cgcatgccct cgacctgctc gccggccatc tcaacgaccc ccgccggacg
     1981 gtgcgccagt ggaccttgca ggcctaccac ttcacgttgg cgagacagga caaggcgctc
     2041 gcggtgaccc aactgaaggc cgcggtggac gggattcgct acgcagatac gaaaaaggcg
     2101 gcggaggatt tgctgaaagc tcttcagcag taactagagc gcggcgtccg tatacagctc
     2161 cagtctccac ccgcgctgga ctgccaacgc ccgcgccaga tccacccgga ggagtccgtc
     2221 gaggaagctc gcgcccagcc ccgcggagat gaggggctgg gcggtgaagg cgaagcggcc
     2281 cacgccggcc caaccggcgt cggagaacag cgccagacgc gcgcccggaa tgccaaaccc
     2341 cagctcggca cgg
//
LOCUS       DASUBP010000062        19812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_723772, whole genome shotgun sequence.
ACCESSION   DASUBP010000062 DASUBP010000000
VERSION     DASUBP010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 19812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19812
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723772"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1242)
                     /locus_tag="VFU75_02555"
     CDS             complement(1..1242)
                     /locus_tag="VFU75_02555"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5465739.1"
                     /translation="MTKTPTVVIVGAGFGGLEAAKNLARAPVRVILIDRNNYHLFQPL
                     LYQVAMAGLEPSSIARPVRRILRGQKNFEFRMAEMTGLDRAAQVVHTSAGDVPYDYLI
                     LAAGGRTNYFGIDSIARNALGLKGLSDAVNIRNHVLSCIEQALLEPDLERRRMLLTFL
                     VVGGGPTGVEMAGALAELIRLVLSRDTRALNIQDTRILLLEAGQRLLPGMRPRFSQPT
                     AEMLWKKHVEVRFGTSVEAFDGERARIAGGEVIPTRTLIWAAGVEAVPLAPMLGVKAL
                     RQGRVAVDPVLHLAGETSVFVIGDSAYLESDGQPLPMLAPVAIQMARSVVGNIRRQIA
                     GQEPRPFQYRDPGVLATIGRNAAVADIRGLAFTGFPAWVVWLVVHIIQLIGFRNKLFV
                     LLDWAWDYFFYDRSARLITRD"
     gene            complement(1245..1586)
                     /locus_tag="VFU75_02560"
     CDS             complement(1245..1586)
                     /locus_tag="VFU75_02560"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HEU5465740.1"
                     /translation="MALSSSMLVSTTFTVEGRRVREYKGLVRGIIVRAPTISQGILGG
                     LKSIIGGNIGAYTEMCEQARTTAYDLMITHAREMGANAILGVSYDTSEIGGDRSAATE
                     VLCYGTAVVLE"
     gene            complement(1607..2062)
                     /gene="panD"
                     /locus_tag="VFU75_02565"
     CDS             complement(1607..2062)
                     /gene="panD"
                     /locus_tag="VFU75_02565"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HEU5465741.1"
                     /translation="MAARLISKRPLKPCYLWRMYLTLCKAKLHRATVTQAELHYEGSI
                     TIDAELLDSAGIVPFERVQVVDVNNGNRFETYTISGKRGSGTICVNGAAARLVHPGDH
                     VIIIAYAQFTPEEATRHQPVIVLLDAKNKVRERITADQSRDIAILSESD"
     gene            2109..2786
                     /locus_tag="VFU75_02570"
     CDS             2109..2786
                     /locus_tag="VFU75_02570"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HEU5465742.1"
                     /translation="MSSTQSLTRSLIGRKVLMAVTGVVLLLFVFAHMAGNLHFFEGPQ
                     AIADYARFLRTVGAPVLGYGQALWIVRIILLVAVVVHIWAAWSVTRASWAARPVGYRQ
                     LEKVETTYAARTMRWGGVIILLFVVYHLMDLTFGSANPSYVHLDPYHNMVASFSRVPV
                     ALSYLVAMAVLCLHMYHGIYSGFQTLGLNHPRYNRFRRGLAGAYAVIVAAGFASVPVS
                     ILAGWMR"
     gene            2789..4699
                     /locus_tag="VFU75_02575"
     CDS             2789..4699
                     /locus_tag="VFU75_02575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HEU5465743.1"
                     /translation="MELKSAVPEGPITEKWERHRFEMKLVNPANKRKHSVIVVGSGLA
                     GASAAASMAELGYQVSCFCFQDSPRRAHSIAAQGGINAAKNYPNDGDSVYRLFYDTVK
                     GGDFRSREANVYRLAQISVQIIDQCAAQGVPFAREYGGLLANRSFGGAQVSRTFYARG
                     QTGQQLLLGAYQALSRQIGLGGVKMHPRTEMLDIIVVNGQARGIVVRDLVSGKISSHL
                     ADAVVLATGGYSNVYFLSTNAKGCNVTATWRAHKRGALFANPCYTQIHPTCIPVHGDH
                     QSKLTLMSESLRNDGRVWVPKAKGDARAPGQIPDADRDYYLERMYPSFGNLVPRDVAS
                     RRAKDMCDDGLGVGGGHQVYLDFRDAVQRLGARGVAERYGNLFEMYQRITGEDPYQVP
                     MRIYPAPHYTMGGLWVDYNLMSSVPGLHVIGEANFSDHGANRLGASALMQGLADGYFV
                     LPYTIGNYLAQAKLAPVSLSDPEVKKVEAEVNEKIKKLLGVRGTKSVDAFHRELGRLM
                     WDECGMSRNRAGLESAIDKVRAMRERFWKDVFVAGAGEDLNQSLEKAGRVADFFEFAE
                     LMCEDALHREESCGGHFREEYQTPDGEAKRDDARFSYVAAWEFAGVGAAPKLSKEPLD
                     FEYVHLAQRSYK"
     gene            4696..5469
                     /locus_tag="VFU75_02580"
     CDS             4696..5469
                     /locus_tag="VFU75_02580"
                     /inference="COORDINATES: protein motif:HMM:NF005746.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HEU5465744.1"
                     /translation="MKLVLHVWRQAGRDAQGGFVRYEVADASPDISFLELLDMLNESL
                     VARREEPIAFDHDCREGICGSCGVVINGRPHGPQRHTTACQLHLRSFQDGQEIWVEPW
                     RARAFPVLKDLMVDRSAFDRVIQSGGFITARTGGAPDANAVLVPKRDADLAMDAAQCI
                     GCGACVAACPNASAMLFVAAKVSHLGLLPQGQPERYSRVVNMVETMDAEGFGACTNHG
                     ECMAACPKGISVDFIARLNRDYLKATLAPPPEGAAWSGA"
     gene            complement(5466..6395)
                     /gene="mdh"
                     /locus_tag="VFU75_02585"
     CDS             complement(5466..6395)
                     /gene="mdh"
                     /locus_tag="VFU75_02585"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HEU5465745.1"
                     /translation="MLNKITVVGAGNVGATAAQRLAEKELARTIVLVDVVEGVPQGKG
                     LDQWESAPIEGFDTRVIGANDYSAAAGSEVFVVTAGIARKPGMSRDDLVRTNADIVKA
                     VSAEIKKASPNAIVVVISNPLDVMCWVAKQVTGFPRERVIGMAGVLDTARYRAFLAEA
                     LDVSVKDIQAMVLGGHGDTMVPLISCTTVAGIPVTQLLAKDKLDAIVTRTRNGGAEIV
                     QYLKTGSAYYAPSAAAAEMVEAIVKDQKRILPCSAWLEGEYGLKGMYSGVPCKIGRKG
                     LEQIVNVELSANEKADLAKSAQSVLDTMKAVSL"
     gene            complement(6444..7355)
                     /locus_tag="VFU75_02590"
     CDS             complement(6444..7355)
                     /locus_tag="VFU75_02590"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HEU5465746.1"
                     /translation="MSALQLRRLAIAVVVLLALWGLSALFAHRSDKVRGALVLPVLPP
                     TLTDTIAILHGRDTVRLAQVAPGVWTANGFPATPGKGSELLKAIGDTSPRDIASISPS
                     SFGRMGLDSTAWTLRVGPAAQPRFTLLVGKSGSEYGTAFVRTPKSDTAYLWRGTMPYL
                     IQRQADTWRDHRIATITPDSVEAIDISLHGKRFTVQKQGSGWTVAGQKADSTKVGILL
                     AQFRTFDAQGFGGLRTADSLRHTTPRGQRAVTVHGKGKTPLLSLTLDSAGGTFWGIRP
                     GDSVAYRIDNYQVMQITPAADSLRAHH"
     gene            complement(7352..9574)
                     /locus_tag="VFU75_02595"
     CDS             complement(7352..9574)
                     /locus_tag="VFU75_02595"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HEU5465747.1"
                     /translation="MNAIKAIARRELRALFDQPTAYILLVVFLGVNDFLCFRQVELYG
                     VASLRPMLDLLPWLLLFLVPAVTMRALAEDARSGIIEVVLAQPITELELLIGKYLGQV
                     LFLWIALAATITIPLGLAFGAHIDIGILIAQYLGAALLAAGLAGIGVWSSSVTKNQVT
                     AFIVGVLVMFALVLVGLDPLLVGLPAALGDVAASLGVLPHFQQIARGVIDLRDAVYFV
                     TLAAVFLALAYLGLAGRKLAPAGEELRRLRLGTSLAVVGLVVLNLFGRHIGGRLDLTP
                     GGEYSLSRATKTMLKQLPDLVTIKLFASSSLPPEIAFLRRDVDDLLGDYRSAGRGKVR
                     VVVLDPTHDSSAARQAEALRIPPVQFNVVGQGSLQMKEGYLGLAVQFANDTRQIPFIR
                     KSDDLEYRLTSFIRELTQTSKPVLGWIRAGEGGSPNGDVSFATVQEALGRSYDLRTIA
                     LAADTSIPAQAKVLVLSGSVDSLPPAQLAPLRAFLARGGNILLMAGGMTRAPQGQFAM
                     SRPVGWNALTRALGVSIRSDMVYDIAANAQVGLPSQFGQVVLPYPLWPRGISTKASVI
                     DAELPSVLLPWTSSIDTAGAPKGTVTPLLVTSKLAGLMQQNVILSPQQDFSRANTAQR
                     ILAVQVQPKSPGAGRLVVVGNGDFASDHFAQNSPENAVFVLNAVDWLAQDEALISIRS
                     KDRTPPPLAFTNAALRGAVRYGNIIGVPLVVALFGALRLARRRARTRQPYRPLAQGAA
                     "
     gene            complement(9571..10506)
                     /locus_tag="VFU75_02600"
     CDS             complement(9571..10506)
                     /locus_tag="VFU75_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5465748.1"
                     /translation="MSLLTLQNVAKRFDGVTAVDDVSFSVDRGEVVGFLGPNGAGKST
                     TMRMITQFLEPDGGTITLDGALLGNDARGSKRRIGYLPESNPLYGDMLVADYLAFIAD
                     LREITGAERARALDEAVTSTGIEPVFYRPISELSKGYRQRTGLAQAILHKPDLLVLDE
                     PTEGLDPNQRVEIRHLISDLGRDRTVLLSTHVLSEVRQSCSRLLIINQGKIVADGGVD
                     ALLQRAEGGVQITVEASGASVAQKLGALAGVTAADPTNTPSGRTRVTLSVRSERDLRP
                     EIFALATREGWTLYELHQEQGSLEALFRQLTGGAA"
     gene            complement(10594..11628)
                     /gene="moaA"
                     /locus_tag="VFU75_02605"
     CDS             complement(10594..11628)
                     /gene="moaA"
                     /locus_tag="VFU75_02605"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HEU5465749.1"
                     /translation="MNLPVLDRMHRPLTSLRVSLTDRCNMRCRYCMPEDEYTWLPRES
                     ILTFEEIVRLVRVFAGLGVRRVRLTGGEPLLRQGLPELVRLLRREVELSDLALTTNGL
                     LLSRWAEALFRAGLDRVTVSLDTLRADRMEAFARTDRHSAALAGIDAACSVGFRSVKV
                     NAVVVRGFNDDEVLDLIEFGRARRVEVRFIEYMDVGGATHWSPEQVVTRAEILDRITT
                     RYGTPEPAPDPGHTAAPAERFRLPDGTPFGIVASTSAPFCGTCDRARLTADGTWFLCL
                     YASRGLDLREALRSGVSDEDLATMIGVTWRDRRDRGAEKRLGMSQRAALIPAAELRAD
                     PHREMHTRGG"
     gene            complement(11625..12062)
                     /locus_tag="VFU75_02610"
     CDS             complement(11625..12062)
                     /locus_tag="VFU75_02610"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HEU5465750.1"
                     /translation="MTYLSTAPLSIEALLAEVAAPHCGGTCAFLGTVRDGPDDGGVTA
                     IEYSAYDAMAEAELDRIVSETRRQWPEARVAVRHRLGLVAAEEASVAIAVATPHRADA
                     FAACRYVIEQVKKRLPVWKKELRADGTATWVDPSGHPSMVGPQ"
     gene            complement(12062..12331)
                     /locus_tag="VFU75_02615"
     CDS             complement(12062..12331)
                     /locus_tag="VFU75_02615"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HEU5465751.1"
                     /translation="MSRVAGAVGTVRIRFFARYAELAGRSEGTLSVPLPATVSDVIQR
                     IRDEWPGTSALPAQPLAALNLRHVRLDAPVADGDELAFLPPLAGG"
     gene            complement(12352..14133)
                     /locus_tag="VFU75_02620"
     CDS             complement(12352..14133)
                     /locus_tag="VFU75_02620"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU5465752.1"
                     /translation="MRHFFFALIALPLSAQTVRYQVSVPDPSNKLFHVEATFPTSGLD
                     TLYVALPAWSPGNYTIQNYARFVRHFGARDDSGRTLFWDRADKTTWRVPTGGATAITV
                     SFDELADTIDLSMARIHGDLAQFLGTNLFLYQPGHLDRPAEVRFAVPEGWRVTTALKS
                     AGHGVYTAPNYDQLADAETFLGHFSVDSLQAGGKWIRIAVWPASDYTPAVQASMRGSV
                     SKIAEVQDRLMGGAPYDVYTVFFDVVRDTINWAGGLEHLYGQFDIMPAGPGFADPNGT
                     LGGFMNPLMSHEFFHLWNVKRIRPVEMWPYEYRDEQYTPLLWWSEGVTDYYADLTNWR
                     AHLWSDSDFLDHVRQNIDQVNSAPEPWSEEDGSEATWIDEVYVNSSQLYYPKGSLTGL
                     LLDISIRDASDNAHSLDDVMRALWTRYYQAGKGFSTANLLDLLRENGMPDVATFYQRY
                     INGRDSLPYAAVFAKAGIDYHVEHTSVPTLGVSTSGANFAVLTGVTPGSVAAAAGLQV
                     GDTLVRIGDVVVSTTADFGPQFRSRYHRKAGSALDIVVHRAGQQLTLHATVREREVES
                     ISVELAKAPTPQQARIWHGLATGTTGR"
     gene            complement(14238..14555)
                     /locus_tag="VFU75_02625"
     CDS             complement(14238..14555)
                     /locus_tag="VFU75_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU5465753.1"
                     /translation="MTKADLVEKVTAQIARTAGPLISKKDCARVVDSFLDAVKAALSE
                     QHNIEIRGFGTFKIRQRKTRMARNPRTGDPVEVAARPVPVFKPSKELRAMVADASGQE
                     VVS"
     gene            complement(14612..15691)
                     /gene="carA"
                     /locus_tag="VFU75_02630"
     CDS             complement(14612..15691)
                     /gene="carA"
                     /locus_tag="VFU75_02630"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEU5465754.1"
                     /translation="MLLEDGAWFPGTAPLPFETTCAEVVFTTNLSGYQEVFTDPSYLG
                     QLVVMTAPMIGNYGINPEDVESDRPRVAGVVVRELSIQPSNWRSTGPLLEWLSDARVP
                     LITDVDTRQLTRHIRSKGAMRGALALGDEPSDDVRRLLAASPDMNGLDLASKATIDAR
                     YSEGPEDAKHHVVAYDFGMKRNILRLFVQNGCRVTVVPASTTAAQVRELQPDGVFLSN
                     GPGDPAAVGYAIDSIRTLADGKLPIFGICLGHQLLGLALGGETVKLPYGHRGGNHPVR
                     DVKTGQVLITSQNHGFAVKGDTGGVPGAKSLEVTHLNLNDGTVEGVRHREAPIFAVQY
                     HPEASPGPHDAQGHFGEFLQALDSQ"
     gene            complement(15708..16076)
                     /gene="secG"
                     /locus_tag="VFU75_02635"
     CDS             complement(15708..16076)
                     /gene="secG"
                     /locus_tag="VFU75_02635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEU5465755.1"
                     /translation="MYTFFLVLLLLDALILGTAVLLQAGQGGGLASLGGGAGTDMFMG
                     GRQTVTLLTKATWWCAGIFLFLALVLAFMSSRTGTPRSVLQGQIQAPASQPAPLPLQS
                     TPAPTTTSQPAAPNTPTPRP"
     gene            complement(16094..16843)
                     /gene="tpiA"
                     /locus_tag="VFU75_02640"
     CDS             complement(16094..16843)
                     /gene="tpiA"
                     /locus_tag="VFU75_02640"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEU5465756.1"
                     /translation="MRRLLFAANWKMHLGPADARAYCTAFLSKSAPIPGREVWFFPPA
                     ASLEAVSNALQGRPDIRVGAQDIHWEPKGAFTGALSAGIVSQAGARATLVGHSERRKI
                     FGETDAETARKVRAALAAGLVPMLCVGEELKEREAGNTLAVVTRQLAGALDGLSAAEV
                     EMVVLAYEPVWAIGTGRNATPQDAGAVHREIRRWLAGHGAPHPQILYGGSVNLGNIAA
                     LLGEAEIDGVLVGGASLEPDGWAQLVRTGAP"
     gene            complement(16840..18021)
                     /locus_tag="VFU75_02645"
     CDS             complement(16840..18021)
                     /locus_tag="VFU75_02645"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEU5465757.1"
                     /translation="MTKRALSDLPKEALDGKRALVRVDFNVPLKNGAVTDDTRIRAAL
                     PTIKYLRDGGARVVLLSHLGRPKGGPDPQYSLKQVVPTLEKLLGAPVEFIADPAAGIA
                     ATKRMPRGGVALVENTRFWPGEEKNDPELAKTFAALGDFYVDDAFGSAHRAHSSTEAV
                     AHILKPAVAGFLMQKELKYLGEALAAPKRPFVAVLGGAKISGKIDVIESLLPRVDEVL
                     IGGAMACTFFAAMGFEVGTSLVERDRIDLAKALLTKAGKKLILPRGAVIAPSLERSGE
                     RTSVASDAIPAGWAVFDIDQATAADFRARILKAKTILWNGPMGVFETPPFDVGTRAVA
                     NALVEAGAKGAVTIVGGGDSAAAVAGLENKLSHVSTGGGASLEFLEGKPLPGVVALED
                     A"
     gene            complement(18018..19031)
                     /gene="gap"
                     /locus_tag="VFU75_02650"
     CDS             complement(18018..19031)
                     /gene="gap"
                     /locus_tag="VFU75_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEU5465758.1"
                     /translation="MAVRVGINGFGRIGRNVLRAAVAAGNTAIEFVGVNDITDTKTLA
                     HLLKYDSVHGKFPGTVEAKPDALIVNGKEIKVSAIKEPEKLPWKDLGVSVVLESTGRF
                     TDRDNANKHLTAGARKVVISAPAKGEDITVVMGVNHTKYDPAKHTVISNASCTTNCLV
                     PVVKVVLDTWGFVHGFMTTVHSYTNDQQILDLPHKDLRRARAAALSIIPTTTGAAKAT
                     SLVIPEVKGKIDGISLRVPTPDVSVVELIAEVSKSTTVEEVNQTFKAAAASGPLKGIL
                     ATSDEPLVSHDYIGSLYSSVVDTPSTAVIDGKLIHITSWYDNEMGYSARCVDLLSYIG
                     SRQ"
     gene            complement(19041..>19812)
                     /locus_tag="VFU75_02655"
     CDS             complement(19041..>19812)
                     /locus_tag="VFU75_02655"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="double zinc ribbon domain-containing protein"
                     /protein_id="HEU5465759.1"
                     /translation="PWSARSVRSALAQLFLPAECLLCHALLPYLQSDRIVCPGCRHRW
                     RPVVPPWCERCGQPEPHFGPCRLCHAWVPAFERVRSAVWLDVPARTAVHALKYGGLPR
                     VAGDLAAVMTRHLPAPPKGAALVPIPLAPKRLARRGYNQSEVLARALGTAWQVPVLSG
                     LLARVRETATQTTLTPASRLANVAGAFELRIDDWGLRIGQRTGQSAIRNPQSPIVLVD
                     DVFTTGATLAAAAQALATGGATHIAAVTFGRAVIPDFT"
BASE COUNT         3285 a         6518 c         6728 g         3281 t
ORIGIN      
        1 tcagtcccgc gtgatcaggc gcgcgctgcg gtcgtagaag aagtagtccc aggcccagtc
       61 gagcaggacg aacagcttgt tgcggaagcc gatgagctgg atgatgtgca ccaccagcca
      121 gaccacccac gcgggaaatc cggtgaacgc caggccccgg atgtcggcca ccgcagcgtt
      181 gcgcccgatg gtggccagca ccccggggtc ccgatactgg aaaggccggg gctcttgtcc
      241 cgcgatctga cggcggatgt tccccaccac ggagcgcgcc atctggatcg cgacgggcgc
      301 gagcatgggg agtggctgtc cgtcggactc gagataggcc gagtccccga tgacgaagac
      361 gctggtctcc ccggccagat ggagcacggg gtccacggct acgcgcccct gccggagcgc
      421 tttcacgccg agcatggggg ccagcgggac ggcctccacg ccggcggccc agatgagcgt
      481 cctggtcggg atgacttccc cgcccgcgat ccgcgcccgt tccccgtcga acgcctcgac
      541 cgacgttccg aaccgcactt cgacgtgctt cttccagagc atctcggcgg tgggttgtga
      601 gaaccgcggc cgcatgccgg ggaggaggcg ctgcccggct tcgagcagca ggatgcgggt
      661 gtcctggata ttcagggccc gggtatcccg ggagagcacg agccggatca gctccgcgag
      721 cgcgccggcc atttccacgc ccgtgggccc cccgcccacg accaggaacg tgagcagcat
      781 ccgccggcgc tcgagatccg gctcgagcaa cgcctgttcg atgcaggaga gcacgtgatt
      841 ccggatgttg acggcgtccg acagcccctt cagacccagt gcgttccggg cgatgctgtc
      901 gatgccgaag taattggtgc ggccgccggc ggcgaggatc agatagtcgt aggggacgtc
      961 accggccgac gtatggacca cctgcgcggc ccggtccagc ccggtcatct ccgccattcg
     1021 gaattcgaag ttcttctgcc cccgcagaat ccggcgcacc gggcgcgcga tggacgaggg
     1081 ttcgagcccc gccatcgcga cctggtagag cagcggctgg aagaggtggt agttgttgcg
     1141 atcgatcaga atgacacgga cgggagcccg cgccaggttc ttggcggcct cgagaccgcc
     1201 gaagcccgcg ccgacgatga cgaccgtcgg ggtcttggtc acgcctactc caggacgacc
     1261 gccgttccgt agcagagcac ttcagtggcg gcggaccggt cgccgccgat ctcggacgtg
     1321 tcgtagctga cgccgaggat ggcgttggcg cccatctcgc gcgcgtgggt gatcatcagg
     1381 tcgtacgcgg tggtgcgggc ttgctcgcac atctcggtgt atgcgccgat gttgccgccg
     1441 atgatcgatt tcagtccgcc caggatgccc tggctgatgg tcggcgcgcg aacgatgatg
     1501 ccgcgcacca ggcccttgta ctcacgcaca cggcggcctt cgaccgtgaa ggtggtggac
     1561 acgagcatcg aggacgagag ggccacgaaa cctcctcagt ggggattcag tcggattcgg
     1621 acaggatggc gatgtctctg gactggtcgg cggtgatgcg ctcgcgaacc ttgttcttgg
     1681 cgtcgagcaa gacgatgacg ggctggtgac gcgtggcctc ttcgggcgtg aattgcgcgt
     1741 aggcgatgat gatgacgtgg tcgcccggat gaaccagccg ggccgccgcg ccgttcacgc
     1801 agatggtgcc cgagccgcgc ttgcccgaga tggtgtacgt ctcgaagcgg tttccgttgt
     1861 tgacgtccac cacctgcacc cgctcgaagg gcacgatgcc cgccgagtcg aggagctcgg
     1921 cgtcgatggt gatcgagccc tcgtaatgca gctcggcttg cgtcaccgtg gcgcgatgca
     1981 gcttggcttt gcacagggtc aagtacatgc gccaaagata gcagggcttc aaaggccgct
     2041 tgctgatgag gcgggcggcc atgtatcatc cgtcgggctt acctggacgc cctttccggg
     2101 gagattggat gtcgtcgacg cagtccctca cccgttcgct catcggccgg aaggtcctca
     2161 tggccgtcac gggcgtggtg ctcctcctat tcgtcttcgc gcacatggcc ggcaacctcc
     2221 atttctttga agggccgcaa gccatcgcgg actacgccag gttcctccgc accgtcggtg
     2281 caccggtgct cgggtacggg caggccctct ggatcgtccg gatcatcctc ctcgtcgccg
     2341 tggtggtgca tatctgggcc gcctggtcgg tgacccgggc ttcctgggcc gcccggcccg
     2401 tcgggtaccg gcagctcgag aaggtcgaga cgacctatgc ggcgcgcacc atgcgctggg
     2461 ggggcgtcat catcctcctc ttcgtcgtct accatttgat ggacctgacg ttcggctcgg
     2521 cgaatccctc ctatgtgcac ctcgatccct accacaacat ggtcgcgagc ttctcccgcg
     2581 tgcccgtggc gctgagctat ctcgtggcca tggcggtgct ctgtctgcac atgtaccacg
     2641 ggatctactc gggctttcag accctggggc tgaaccatcc ccgctacaac aggttccggc
     2701 ggggattggc gggcgcctac gccgtcatcg tggcggccgg cttcgcctcg gtgccggttt
     2761 cgattcttgc gggctggatg aggtaatcgt ggagctcaag agcgccgtcc ccgaaggacc
     2821 gatcaccgag aagtgggagc gccaccgctt cgagatgaag ctggtgaacc ccgcgaacaa
     2881 gcggaaacac tccgtcatcg tggtcgggtc gggcctggcg ggcgcctcgg ccgccgcgtc
     2941 gatggcggag ctcggctacc aggtctcgtg cttctgcttc caggattctc cccgccgcgc
     3001 ccactcgatc gcggcgcaag gcgggatcaa cgccgccaag aactatccca atgacggtga
     3061 ttccgtctac cggctcttct acgacaccgt gaagggcggc gacttccgct cccgagaggc
     3121 caacgtctac cggctggccc agatctcggt gcagatcatc gatcagtgcg ccgcgcaggg
     3181 cgtcccgttt gcccgcgagt acggcggcct gctcgccaac cgctcgttcg gcggcgcgca
     3241 ggtctcccgg accttctacg cccgcggcca gacgggccag caactcctgc tcggcgcgta
     3301 ccaggcgctg tcgcggcaga tcgggctcgg cggcgtgaag atgcatccgc gcaccgaaat
     3361 gctcgacatc atcgtcgtga acgggcaggc gcgcgggatc gtcgtccgcg acctcgtctc
     3421 gggaaagatc agctcacatc tcgccgacgc ggtggtgctg gcgacgggtg ggtacagcaa
     3481 cgtgtacttc ctctccacca acgcgaaggg ctgcaacgtc accgccacct ggcgggcgca
     3541 caaacgcggc gccctgttcg cgaacccctg ctacacccag atccacccga cgtgcatccc
     3601 cgtccatggg gaccaccagt cgaaactgac cctgatgagc gagtctctcc ggaacgacgg
     3661 ccgggtgtgg gtgccgaagg ccaagggaga tgcccgcgct cccggccaga tccccgacgc
     3721 cgatcgcgac tactacctgg agcggatgta tccgtcgttc gggaatctgg tgccccgcga
     3781 cgtcgcgagc cggcgcgcga aggacatgtg cgacgacgga ctgggcgtcg gcggcgggca
     3841 ccaggtctac ctcgacttcc gggacgcggt gcagcggctc ggcgcgcggg gcgtggcgga
     3901 gcggtacggc aacctcttcg agatgtacca gcgcatcacg ggagaagacc cctatcaggt
     3961 cccgatgcgc atttacccgg cgcctcacta cacgatgggg gggctctggg tggactacaa
     4021 tctgatgagc tccgtcccgg ggctgcacgt gatcggcgaa gcgaacttct cggatcacgg
     4081 ggccaaccgc ctgggcgcga gcgcgctgat gcagggcctg gccgacgggt acttcgttct
     4141 gccctacacc atcgggaact acctcgcgca ggcgaaactg gctccggtct ccctgtcgga
     4201 ccccgaagtg aagaaggtcg aggccgaggt gaacgagaag atcaagaagc tcttgggcgt
     4261 caggggcacg aagagcgtcg atgcgttcca ccgcgagctg ggccggctga tgtgggacga
     4321 atgcggcatg tcgcggaacc gggccggact cgagtcggcg atcgacaagg tccgcgcgat
     4381 gcgggagagg ttctggaagg acgtgttcgt cgcgggggcc ggcgaggacc tgaatcaatc
     4441 tctggagaaa gcgggccgcg tggccgactt cttcgagttc gccgagctca tgtgtgaaga
     4501 cgcgctccac cgcgaggagt cgtgcggggg gcacttccgg gaggagtacc agacgccgga
     4561 cggcgaagcg aagcgtgatg acgcgcgctt ctcgtacgtg gcggcgtggg agttcgccgg
     4621 cgtcggcgcc gccccgaagc tctccaagga gccgctggat ttcgagtacg tccacctcgc
     4681 ccaacggagc tacaagtgaa gctcgttctc cacgtgtggc ggcaggcggg ccgggacgcc
     4741 cagggcggct tcgtccggta cgaggtggct gacgcgtctc cggatatttc gttcctcgag
     4801 ctcctcgata tgctcaatga atccctcgtg gcgaggcgcg aggagccgat cgcgttcgat
     4861 catgactgtc gtgaggggat ttgcgggagc tgcggggtgg tcatcaacgg ccggccgcat
     4921 ggcccgcagc ggcacacgac cgcctgccag ctccatctcc ggagcttcca ggatggccag
     4981 gagatttggg tggagccgtg gcgcgctaga gcattcccag tattgaaaga tctgatggtg
     5041 gaccggagcg cgttcgaccg ggtgattcag tcaggcgggt tcatcaccgc ccgcaccggc
     5101 ggcgcacccg atgccaatgc ggtgctcgtg cccaagcgcg atgcggacct ggcgatggac
     5161 gccgcccagt gcatcggctg cggtgcctgc gtcgcggcct gccccaacgc atccgcgatg
     5221 ctcttcgtgg cggccaaggt gagtcacctg gggctcctgc cccagggaca gcccgagcgc
     5281 tactcgcggg tggtcaacat ggtcgagaca atggatgcgg agggcttcgg cgcctgcacc
     5341 aatcacggcg agtgcatggc cgcgtgcccg aaggggatca gcgtggactt catcgcgcgg
     5401 ctgaaccgcg actacctcaa ggccaccctg gcgcccccgc ccgagggcgc cgcgtggagt
     5461 ggcgcctaga gcgacaccgc cttcatggta tcgaggaccg actgggccga cttcgcgagg
     5521 tcggcctttt cgttggccga cagctcgacg ttgacgattt gctcgaggcc cttccgcccg
     5581 atcttgcacg gcacgccgga gtacatgccc ttcagcccgt attccccttc gagccacgcg
     5641 gagcacggca ggatccgctt ctgatccttg acgatggctt ccaccatctc cgccgcggcg
     5701 gccgacgggg cgtaataggc tgatccggtt ttcagatact ggacgatctc cgcgccgcca
     5761 ttccgcgtgc gcgtcacgat ggcgtcgagc ttgtccttgg ccaggagctg ggtgacgggg
     5821 atcccggcga ccgtcgtgca ggagatcagg ggcaccatcg tgtccccatg acctccgagc
     5881 accatcgcct gaatgtcctt cacggacacg tcgagcgcct cggccaggaa cgcgcgatag
     5941 cgcgccgtgt cgagtacccc cgccatgccg atcacccgct cgcgcgggaa gccggtcacc
     6001 tgcttggcga cccagcacat gacgtcgagc ggattcgaga tcaccacgac gatggcgttc
     6061 ggggacgcct tcttgatctc ggccgaaacc gccttcacga tgtccgcgtt ggtgcggacc
     6121 aggtcgtcgc ggctcatccc cggtttgcgg gcgatgccgg ccgtgacgac gaagacttca
     6181 ctgcccgccg ccgccgagta gtcgttcgcg ccgatgacgc gggtgtcgaa cccctcgatg
     6241 ggggctgact cccactggtc caggcccttc ccctgcggga caccctcgac gacgtccacc
     6301 agaacgatgg tccgggccaa ctccttctcg gccaggcgct gggccgccgt cgctccgacg
     6361 ttccccgcgc cgaccaccgt gatcttgttc agcatgtgat gaaatccccg gaatgggtgc
     6421 gagcaaaagc taacatcgcc cgcctagtgg tgcgcgcgga gactgtcggc cgcgggcgtg
     6481 atctgcatga cctgatagtt gtcgatccgg tacgcgaccg aatctccggg ccggatgccc
     6541 cagaacgtgc cccctgccga atcgagggtg agcgacagca acggcgtctt gcctttcccg
     6601 tgcacggtga ccgcgcgctg cccgcggggg gtcgtgtggc gcagcgaatc cgcggtgcgc
     6661 aggccgccga atccttgtgc gtcgaacgtg cggaactgcg cgagcaggat tcccaccttg
     6721 gtggaatccg ccttctggcc cgcgacggtc caacccgagc cttgcttctg cacggtaaag
     6781 cgcttgccgt gcaggctgat atcgatcgct tcgacggagt ccggggtgat cgttgcgatc
     6841 cggtgatctc gccaggtatc ggcctgccgc tggatcaggt acggcatcgt cccccgccag
     6901 aggtaggcgg tatcggattt cggcgtgcgg acgaacgccg tgccgtattc ggaacccgac
     6961 ttgccgacca ggagggtgaa gcgcggctgt gcggcgggcc ccacgcgaag cgtccaggcg
     7021 gtgctgtcga gccccatgcg cccgaaggat gatgggctga tcgaggcgat gtcccgcggg
     7081 gacgtgtcac cgatcgcctt gagcagctcg cttcccttcc ccggcgtcgc ggggaagccg
     7141 ttcgcggtcc agacgccggg agccacctgc gccagccgga ccgtatcccg cccgtgcaga
     7201 atggcgatgg tgtccgtcag cgtcggcggc aggaccggca acaccagggc gccgcgcacc
     7261 ttgtccgagc gatgggcgaa gagcgccgag aggccccaga gcgcgagcag gacgaccacc
     7321 gcgatggcga gacgccggag ttggagggcg ctcatgcggc cccctgagcg agcggacggt
     7381 acggctggcg ggtgcgcgcg cgccgccggg cgaggcggag cgctccgaag agcgcgacga
     7441 ccagtggtac gccgatgatg tttccgtacc gcacggcgcc ccggagcgcg gcgttggtga
     7501 acgccaaagg cggtggcgtc cggtccttgg agcggatgct gatcaacgcc tcatcctgcg
     7561 ccagccaatc gaccgcgttc agcacgaaga cggcgttttc cggagagttc tgggcgaagt
     7621 gatcactggc gaaatccccg ttgcccacga cgaccagacg gccggctccg ggcgacttcg
     7681 gctgcacttg cacggccagg attcgctgcg ccgtgttggc gcgcgagaaa tcctgctggg
     7741 gagagaggat cacgttctgc tgcatgagcc ccgcgagctt cgaggtcacc agaagcgggg
     7801 tcaccgtacc cttgggggca ccggcggtgt cgatcgagct cgtccacggc agcagcacgc
     7861 tgggcagctc agcgtcgatg acggacgctt tggtggaaat tccgcgaggc cagagcggat
     7921 agggcagcac gacctgtccg aactggctcg gcaacccgac ctgggcgttg gccgcgatgt
     7981 cgtagaccat gtcggacctg atgctcacac ccagcgctcg cgtgagggcg ttccagccca
     8041 cggggcgcga catggcgaat tgtccctgcg gtgcccgagt catcccccct gccatgagca
     8101 ggatgttgcc tccgcgggcg aggaatgccc gcagtggcgc gagctgggcc gggggcaagc
     8161 tgtccaccga accggagagc accagcacct tggcctgagc cggaatgctc gtgtcggccg
     8221 cgagcgcgat ggtccggagg tcgtagctgc gtccgagggc ttcctgtacg gtcgcgaagc
     8281 tcacgtctcc gttcggcgac cccccctccc cggcacgaat ccaccccagc accggcttcg
     8341 aggtctgcgt gagctcgcgg atgaaggacg tgagccggta ttcgaggtcg tcggatttcc
     8401 ggatgaaggg gatctgccgc gtgtcgttcg cgaactggac ggcgaggcca aggtagccct
     8461 ccttcatctg cagagacccc tggcccacga cgttgaactg caccggcgga atccgcagcg
     8521 cctcggcctg gcgggccgcg gagctgtcgt gcgtggggtc cagtaccacc acgcgcacct
     8581 tcccccgccc cgccgagcgg tagtcaccca agaggtcatc cacgtcccgc cgcaggaacg
     8641 cgatctcggg cggcaaggag ctcgacgcga agagcttgat cgtcacgagg tccgggagct
     8701 gcttcagcat ggtcttcgtg gcgcgcgaca ggctgtactc gccgcccgga gtgagatcga
     8761 gccggccgcc gatgtgacgg ccgaagaggt tcagtaccac caacccgacg accgccaggc
     8821 tggtgccgag ccggagccgg cgcagctcct cgcccgcggg cgcgagcttt cgcccggcga
     8881 gacccaggta ggcgagtgcc aggaagacgg cggccagcgt cacgaagtag accgcgtccc
     8941 gcaggtcgat gaccccgcgg gcgatctgct ggaagtgcgg gagcacgccg agtgacgcgg
     9001 cgacgtcgcc cagcgcggcg ggcaatccca cgagcagggg gtccaggccg acgagcacca
     9061 gcgcgaacat caccagcaca ccgacgatga aggccgtcac ctggttcttg gtcacgctgg
     9121 aggaccacac gccgatgccg gccagacccg cggccagcaa cgccgccccg aggtactgcg
     9181 cgatcagaat cccgatatcg atgtgcgccc cgaatgcgag accgaggggg atcgtgatcg
     9241 tggcggcgag ggcgatccag aggaacagga cctgccccag gtacttgccg atcaagagct
     9301 cgagctcggt gatcggctgc gccaggacca cctcgatgat ccctgaccgt gcgtcttccg
     9361 ccagcgcgcg catggtgacg gcgggcacca ggaacagcag gagccacggc aggagatcga
     9421 gcatgggccg gagcgacgcg acgccgtaca gctcgacctg ccggaaacag aggaagtcgt
     9481 tcacgcccag gaacaccacc agcaggatgt acgcggtggg ctggtcgaag agggcgcgaa
     9541 gctcgcgccg cgcaatcgcc ttgatggcgt tcatgccgcg ccccccgtga gctggcggaa
     9601 gagcgcttcc agactcccct gttcctggtg cagctcgtag agcgtccagc cttcgcgcgt
     9661 ggccagggcg aagatctccg gtcgcaggtc ccgctccgac cggacggaga gcgtcacccg
     9721 ggtccggccc gacggcgtgt tggtgggatc cgcggcggtc accccggcga gcgcgccgag
     9781 cttctgggcg acactcgctc ccgaggcttc gacggtgatc tgcacacccc cctcggctcg
     9841 ttgcagcagg gcgtcgacac cgccatccgc cacgatcttg ccctgattga tgatcaggag
     9901 gcgcgagcag ctctggcgaa cttcggagag tacgtgggtg gagagcagga cggttcgatc
     9961 ccgccccaga tcggagatga ggtgacggat ctcgacccgc tgattggggt ccagtccttc
    10021 ggtgggctcg tcgaggacca gcaggtccgg tttgtggaga atcgcctgcg cgagcccggt
    10081 gcgctggcgg taccccttgg aaagctcgct gatcgggcgg tagaacaccg gctcgattcc
    10141 ggtggatgtc accgcttcat ccaatgcccg cgcccgctcc gcgcccgtga tctctcgcag
    10201 gtcggcgatg aaggccaggt agtcggcgac gagcatgtcg ccgtagagcg ggttgctctc
    10261 ggggaggtag ccgatgcggc gcttcgagcc acgcgcgtcg ttgccgagga gggcgccgtc
    10321 gagcgtgatg gtgccgccat cgggctcgag aaattgggtg atcatgcgca tggtggtcga
    10381 cttgccggca ccgttggggc ccagaaagcc cacgacttcg ccccggtcga ccgagaagga
    10441 cacgtcgtcg accgcggtca caccgtcgaa ccgcttcgcg acgttctgca atgtgagcaa
    10501 tgacatgtgt gtggagtgtg ctggtggagt cgccgagcat cgtaactact cccggcgccg
    10561 gatggaagcc gtttcgatgc gaagcggagg cgactatccg ccccgggtgt gcatctcccg
    10621 gtgggggtcc gcgcgcaact cggctgctgg gatgagcgcc gcccgttgcg acatgccgag
    10681 gcgcttctcc gcgccgcggt cacggcggtc ccgccaggtc acgccgatca tggtcgccag
    10741 gtcctcgtcg ctcaccccgc tgcgcagggc ctcgcggagg tccaaccccc gcgaagcgta
    10801 gaggcacagg aaccaggtac catccgcggt gagccgggcg cggtcgcagg tgccacagaa
    10861 cggcgcactc gtggacgcga cgatgccgaa aggagtgccg tcgggaaggc ggaatcgctc
    10921 ggccggtgcg gcggtgtgcc cggggtccgg ggcgggctcg ggcgtcccat accgagttgt
    10981 gatgcggtcg agtatctcgg cccgtgtgac cacctgttcg ggggaccagt gggtggcgcc
    11041 acccacatcc atgtactcga tgaaccggac ctcgactctg cgcgcccggc cgaattcgat
    11101 caggtccaga acttcgtcgt cgttgaagcc ccgcaccacc acggcattca ccttgaccga
    11161 gcggaacccg acgctgcaag ccgcgtcgat ccccgccagc gccgcgctgt ggcggtcagt
    11221 gcgcgcgaac gcttccatcc ggtcggcgcg cagcgtgtcc aggctgaccg tgacgcggtc
    11281 gagtccggcg cgaaacagtg cctcggccca gcggctgagg agcagcccgt tggtcgtcaa
    11341 cgccagatcg gacagctcca cttccctccg gaggagccgc acgagttcgg gaaggccctg
    11401 ccggagcaac ggctctcctc cggtgagccg gacgcggcgc acgcccaggc cggcgaacac
    11461 ccgtaccagc cggacgattt cctcgaaggt caggatcgac tcgcggggca gccaggtgta
    11521 ttcatcctcc ggcatgcagt accggcagcg catgttgcag cggtcggtca gcgagacgcg
    11581 caggctggtg agggggcggt gcatgcggtc cagaaccgga aggttcactg cggccccacc
    11641 atggagggat gtcccgacgg gtccacccag gtcgcggtcc cgtccgcccg cagctctttc
    11701 ttccagacgg ggagccgctt cttgacttgc tcgatcacgt accggcaggc tgcgaaggcg
    11761 tcggcccggt gcggtgtcgc tacggcgatc gcgacgctcg cctcctcggc ggccaccaac
    11821 cccagccggt gccgcacggc cacccgcgcc tcgggccatt gacggcgcgt ctccgacacg
    11881 atccggtcca gctccgcttc ggccatcgcg tcgtaggcgg agtactcgat ggcggtcacg
    11941 cctccatcgt cagggccgtc gcgtacggtt cccagaaacg cgcacgtccc gccgcagtga
    12001 ggggcggcga cctccgccag gagtgcctca atcgagaggg gggccgtcga caggtaggtc
    12061 attacccgcc cgcgagtggc ggcaagaagg ccaactcgtc gccgtcggcg accggggcgt
    12121 cgagacggac gtgacgcaga ttgagcgcgg cgaggggctg ggccggaagg gcggacgtcc
    12181 ccggccattc atcccggatc cgctggatca cgtccgagac cgtggcgggg agcggcaccg
    12241 acagcgtgcc ctcgctgcgc cccgccagct cggcgtagcg cgcgaagaac cggattcgca
    12301 ccgtccccac tgctcccgcg acgcgactca tcggggagaa cctacaggct tctaccggcc
    12361 ggtggtaccc gtggcgagtc cgtgccagat cctggcttgt tggggggtcg gtgccttcgc
    12421 cagttccaca ctgatgcttt cgacctcccg ctcacggacc gtcgcgtgga gcgtgagctg
    12481 ctgaccggcg cggtgcacta cgatgtcgag ggccgagccc gccttcctgt gataccggga
    12541 gcggaactgg gggccgaagt cggcggtggt ggatacgacg acgtccccga tccggaccag
    12601 ggtgtccccc acttgcagcc ccgcggcggc ggccacgctc ccgggcgtca cgcccgtcag
    12661 cacggcgaaa ttggcacccg acgtggagac cccaagggtc ggcacgctcg tgtgctcgac
    12721 gtgatagtcg attccggctt tcgcgaacac cgccgcgtag ggcagtgaat cgcggccatt
    12781 gatgtagcgc tggtagaagg tcgccacgtc gggcattccg ttctcgcgga gcagatccag
    12841 cagattcgcc gtcgagaagc ccttccccgc ctggtagtac cgcgtccaga gcgcgcgcat
    12901 cacatcgtcc agggaatgcg cgttgtcgga tgcatcgcgg atggagatgt cgagcaggag
    12961 tccggtgagg gagcccttgg gataatagag ctgcgagctg ttgacgtaga cttcgtcgat
    13021 ccaggtggcc tcggagccat cctcttcgct ccagggctcg ggcgcggaat tcacctgatc
    13081 gatgttctgg cggacgtgat ccagaaaatc cgagtcgctc cagaggtgcg cacgccagtt
    13141 cgtgagatcg gcgtagtaat cggtcacgcc ctcggaccac cagagcagcg gcgtgtattg
    13201 ctcgtcccgg tattcgtacg gccacatctc aaccggcctg atccgtttga cgttccagag
    13261 gtggaagaac tcgtgggaca tgagcggatt catgaacccg ccgagggtac cgttggggtc
    13321 ggcgaacccg gggcccgccg gcatgatgtc gaactgcccg tagaggtgct cgagtccccc
    13381 cgcccagttg atcgtgtccc gcacgacgtc gaagaagacg gtgtagacgt cgtagggtgc
    13441 gcctcccatc aaccggtctt gcacctccgc gatcttcgac acggacccgc gcatcgaggc
    13501 ctgcaccgcc ggcgtgtaat cggacgccgg ccacaccgcg atacggatcc acttgccgcc
    13561 cgcctgcaac gagtcgacgc tgaagtgccc gaggaacgtt tcggcatccg cgagctggtc
    13621 gtaattcggc gccgtgtaca cgccatggcc ggcggatttc aacgccgtcg tcacccgcca
    13681 accctccggg acggcgaacc gcacctccgc gggacgatcc aaatgcccgg gctggtacag
    13741 gaacagattg gtgccgagga actgcgcgag gtcgccgtgg atgcgggcca tcgagagatc
    13801 gatcgtgtct gccaactcgt cgaaggagac ggtgatcgcg gtggcaccgc ctgtcggtac
    13861 ccgccacgtc gtcttgtccg cgcgatccca gaacaacgtc cgccccgagt cgtcacgtgc
    13921 gccgaagtgc cgaacgaagc gggcgtaatt ctgaatcgtg tagttccccg ggctccacgc
    13981 cggcaacgcc acgtagagcg tgtccagacc tgaggttggg aacgtggctt cgacgtggaa
    14041 cagcttgttg ctgggatcgg gaaccgagac ctggtaccgg acggtttgcg cggagagcgg
    14101 taaagcgatg agggcaaaga agaagtggcg cacgacaaag ctccggtttg ttggcgaacg
    14161 acatggaagg ggcttaaaac aatgcggccc cgacgtttag tcggagccgg gagtttcact
    14221 cgagcgatga atggtgctta ggagacgact tcctgcccac tcgcgtctgc gaccatcgca
    14281 cgcaactctt tgctgggctt gaagacaggc accggccgag ctgcgacttc taccggatcg
    14341 cccgtacggg ggttccgggc catacgggtc ttccgctggc ggatcttgaa ggtgccaaaa
    14401 ccgcgtatct ctatgttgtg ctgctcagat agcgctgcct tcaccgcgtc caggaaggag
    14461 tcgaccacgc gggcgcagtc cttcttggaa atcagaggac ccgcagtacg cgcgatttgg
    14521 gcggtcacct tctcgacgag atctgccttc gtcatccgtt ccccctgagg gcacaagggt
    14581 accaagaccg gggggcatat tacgagctag cctattgact gtcaagagct tggaggaact
    14641 ctccgaagtg tccctgggcg tcatgggggc ctggggaggc ctcaggatgg tattggacgg
    14701 cgaaaatcgg ggcttcccgg tgacgcaccc cttcgacggt cccatcgttg aggttgagat
    14761 gcgtgacctc aagtgacttc gcaccgggga cccccccggt gtcacccttc accgcaaaac
    14821 cgtggttctg ggacgtgata agtacctgtc ccgtcttcac atcccgaacg ggatggttcc
    14881 cgccccggtg tccgtacggg agcttcaccg tctccccacc caatgccagc ccgagcagct
    14941 gatggccgag acaaatgccg aagatcggga gcttgccgtc cgcgagggtg cgaatgctat
    15001 cgatcgcgta ccccaccgcc gcggggtccc cggggccatt cgagagaaac acgccgtccg
    15061 gctggagttc gcggacctga gccgcggtcg tcgacgcggg gaccaccgtg acccggcatc
    15121 cgttctggac gaacaaccgg agaatgttgc gtttcatccc gaagtcgtac gcgaccacgt
    15181 gatgcttcgc gtcctccggg ccttcggaat agcgggcgtc gatcgtcgcc ttactggcga
    15241 gatcgaggcc gttcatgtcg ggcgaggccg ccagcagccg gcgcacgtcg tcactgggtt
    15301 cgtcgcccag cgcgagggcc ccgcgcatcg cacccttcga gcggatgtga cgcgtgagct
    15361 gacgggtgtc gacgtcggtg atgagcggga cgcgcgcgtc cgagagccac tcaagtaacg
    15421 gaccggttga gcgccaattc gagggctgga tcgacagctc gcgcacgacc acacccgcga
    15481 cgcgtggccg gtccgactcg acgtcctcgg gattgatccc gtagttgccg atcatcggcg
    15541 ccgtcatcac gacgagctgt ccgagatacg agggatcggt aaagacctcc tgatatccgc
    15601 tcagattcgt ggtgaagacg acttcagcac acgtggtctc gaatgggagt ggcgcggttc
    15661 cggggaacca ggcgccatcc tcgaggagca cgtaagccgg tttcagatca ggggcgcggt
    15721 gtgggggtgt tgggtgccgc gggttgcgac gtggtggtcg gagccggcgt gctctgcaac
    15781 ggcaacggag ccggttggga cgccggtgcc tgaatctggc cctggaggac cgagcgtggc
    15841 gtcccggtgc gggacgacat gaaagccagg acgagtgcga ggaacaggaa gatacccgcg
    15901 caccaccacg tggccttggt cagaagcgtg accgtctgcc ggccgcccat gaacatgtcg
    15961 gtgcccgcgc cgccgccgag ggacgcgagt cccccaccct gaccggcctg gaggagcacc
    16021 gcggtgccga ggatgagggc gtcgagcagc aggagaacga ggaagaaggt gtacatgtcg
    16081 gggcgtatga tacctagggg gcgccagttc gcacaagttg tgcccagcca tcgggttcca
    16141 gtgaggcccc acccaccaaa acgccgtcaa tttcggcttc gcccaagagc gccgcgatgt
    16201 tgccgaggtt cacgctcccc ccatagagga tttgggggtg aggggcacca tgccccgcca
    16261 gccacctccg gatctcccgg tgtaccgcgc cggcatcctg aggggtggcg ttccgtccag
    16321 ttccgatggc ccaaaccggc tcgtacgcca gcaccaccat ctccacctcg gcggcgctga
    16381 gtccatcgag ggctcccgcg agctgtctgg tcacgacggc cagggtgttc cccgcctccc
    16441 gctccttcaa ctcctcaccc acgcacagca tggggaccaa cccggcggca agtgcggccc
    16501 ggactttccg ggcggtctcc gcatcggtct ctccgaagat cttccggcgt tccgagtgtc
    16561 cgaccagggt cgcccgcgca cccgcttgag aaacgattcc cgccgacagc gcgccggtga
    16621 aggcgccctt cggctcccag tggatgtcct gcgcgccgac ccggatgtcg ggccggcctt
    16681 gcagcgcatt ggagaccgct tcgagagacg cagcgggcgg aaagaaccag acctcacgtc
    16741 cggggattgg cgccgatttg gagagaaagg ccgtgcagta ggctcgggca tcagccggcc
    16801 cgaggtgcat tttccagttt gccgcgaaga gcagtcttct cacgcgtctt cgagggccac
    16861 gacgccgggg agaggcttcc cttccagaaa ctccagtgac gcgcctccgc cggtcgagac
    16921 gtgcgagagc ttgttctcga gtccggcgac cgcggccgcg gagtcaccgc cgcccacgat
    16981 ggtgacggct cccttggcgc ccgcttcgac gagcgcgttc gccaccgcgc gggtaccgac
    17041 gtcgaatggc ggggtctcga agacgcccat cgggccattc cacaagatcg tcttggcctt
    17101 gaggatccgg gctcggaagt ccgcggcggt cgcctggtcg atgtcgaaga ccgcccagcc
    17161 ggcggggatg gcgtccgaag cgacgctcgt ccgctcgccg gaccgctcga gggaaggggc
    17221 gatcacggcg ccccggggca gaatcagctt cttccccgcc ttggtcagga gggccttcgc
    17281 gagatcgatc cggtcacgct cgaccaggga ggtcccgact tcgaacccca tcgcggcgaa
    17341 aaacgtgcac gccatcgcgc cgccgatcag cacttcgtcc acgcgcggca ggagtgattc
    17401 gatcacgtcg atcttgcccg agatcttggc gccacccagc acggcgacga agggccgctt
    17461 gggagcggcc agggcctcac cgaggtactt gagctccttc tgcatcagga accccgccac
    17521 ggccggcttg agaatgtgcg cgaccgcctc cgtggagctg tgcgcgcgat gcgccgaccc
    17581 gaacgcatcg tccacgtaga aatccccgag ggccgcgaac gtcttcgcca gctccggatc
    17641 gttcttctct tctccgggcc agaagcgcgt gttctccacc agggccacac cgcctcgcgg
    17701 catgcgcttg gtggccgcga tgcccgccgc ggggtcggcg atgaactcga cgggtgcgcc
    17761 gagcagcttc tcgagcgtcg ggacgacctg cttgagggag tactgcgggt cgggcccgcc
    17821 cttcggccgc ccgagatgcg agagcagcac cacccgcgcg ccgccgtcgc ggagatactt
    17881 gatcgtgggg agcgcggcac ggattcgggt gtcatcggtc acggcgccgt tcttgagcgg
    17941 cacgttgaaa tccacccgga ccagggcacg cttgccgtcg agcgcctcct tggggaggtc
    18001 gctcaacgcc cgcttggtca ctgcctggat ccgatgtacg agagcagatc gacgcagcgc
    18061 gccgagtacc ccatctcgtt gtcgtaccag ctggtgatgt ggatgagctt gccgtcgatc
    18121 accgccgtgg acggcgtgtc gaccacgctc gagtacaatg agccgatgta gtcgtgcgaa
    18181 accagcggct catcggacgt ggccaggatt cccttgagcg ggccgctcgc cgcggccgcc
    18241 ttgaaggtct ggttgacctc ctcgacggtc gtggacttcg acacttcggc gatgagctcc
    18301 acgaccgata cgtcgggagt cgggacgcgc agagagatgc cgtcgatctt ccccttcact
    18361 tccgggatca ccaggctggt ggccttggca gctccggtcg tggtcgggat gatcgagagc
    18421 gccgcggcgc gcgcccggcg gaggtccttg tgcggcaggt cgaggatctg ctgatcgttc
    18481 gtgtagctgt ggaccgtcgt catgaagcca tgcacgaagc cccacgtgtc gagcaccacc
    18541 ttcacgacgg gcaccaggca gttcgtggtg cacgacgcgt tcgagatgac ggtgtgcttg
    18601 gcgggatcgt acttcgtgtg gttgacgccc atcacgaccg tgatgtcttc gcccttggcg
    18661 ggcgcggaaa tcaccacctt cctggccccg gccgtcagat gcttgttggc gttgtctcgg
    18721 tccgtgaagc gcccggtgct ttcgagcacc acgctcactc cgagatcctt ccacgggagc
    18781 ttttccggtt ccttgatggc ggagaccttg atctccttgc cgttcacgat cagggcgtcc
    18841 ggcttggcct cgacggtgcc ggggaatttc ccgtggaccg agtcgtactt cagaaggtgc
    18901 gccagcgtct tggtgtcggt gatgtcgttg acgccgacga actcgatcgc ggtgtttccg
    18961 gcggcgaccg ccgcccgcaa cacgttgcgg ccgatccggc cgaacccgtt gatgccaacc
    19021 cgcactgcca ttgcgcgctc ctacgtgaag tcgggaatga cggcgcggcc gaacgtgacg
    19081 gccgcgatgt gagtggcgcc ccccgtggcc agggcctggg cggccgccgc gagcgttgcg
    19141 ccggtggtaa agacgtcgtc caccaggaca attggcgatt gtggatttcg gattgcggat
    19201 tgtccggtac gctggccaat ccgcaatccc caatcgtcaa tccgcaattc gaaagctccc
    19261 gccacgttcg ccagacgcga cgctggtgtc aacgtggtct gcgtcgcggt ctcacgcacc
    19321 cgcgccagca acccactcaa caccggcact tgccacgccg ttcccagtgc gcgcgccagc
    19381 acttcactct ggttgtatcc acgccgcgcc aggcgcttcg gggcgagcgg gatgggcacc
    19441 agcgcggcac ccttcggggg cgccggcagg tggcgcgtca tcaccgccgc gaggtccccc
    19501 gcgacccgcg gcagtccccc gtacttgagg gcgtgcaccg ccgttcgggc aggcacgtcg
    19561 agccacacgg cggaacggac acgttcgaac gcggggaccc aggcgtgaca gaggcggcaa
    19621 ggaccgaagt ggggctccgg ctgcccgcaa cgctcgcacc agggcggcac cacggggcgc
    19681 cagcggtgac ggcacccagg gcacacgatc ctgtcggatt ggaggtacgg cagaagggcg
    19741 tggcagagaa ggcactcggc cggcagaaac agctgggcga gcgcgctacg cacggagcgc
    19801 gcgctccacg gc
//
LOCUS       DASUBP010000063         6025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_124116, whole genome shotgun sequence.
ACCESSION   DASUBP010000063 DASUBP010000000
VERSION     DASUBP010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124116"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1299
                     /locus_tag="VFU75_02660"
     CDS             <1..1299
                     /locus_tag="VFU75_02660"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5465760.1"
                     /translation="LKKDGTPLWSLLTTSLVRTADGGPAFFITVIEDLSERKRLEAQL
                     LHAQKLEAVGQLAGGVAHDFSNLIGAIQGYAQLVERTLAPEDPRRADIGEIRKAGQRA
                     AGLIHQLLTFARRQETQPQLVDVNGLVTNLGRLVSRVLGSNIKVSWGLNEDLPAIKVD
                     PGQFEQVILNLAINARDAMPDGGTLGIETSLVQVDQSHVNRQRGSRPGPHVMVTVSDT
                     GTGMPREVLDRIFEPFFTTKGPDRGTGLGLAICYGIVKQAGGNIWVYSEPGHGTTFRL
                     YFPAVLEGAGAMSLPESDPELPGGSERLLLVEDDPQMRALALRVLSDCGYDVTATPDA
                     ETAMAEVASGGLDPALLVTDLILPGVTGLQMAQALRAEKPGLRVLCMTGQADRVSSSS
                     RRQQGFGLIEKPFTAQALARAVRDALDQQPSAISDQPSAN"
     gene            1350..2210
                     /locus_tag="VFU75_02665"
     CDS             1350..2210
                     /locus_tag="VFU75_02665"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HEU5465761.1"
                     /translation="MPETPLVSHISDTARWVAMYRAFESERPDAIFHDPYARRLAGPV
                     GERIAAAMGGTKWAWPMIVRTAVMDEVIQRVVGEGADTVLNLAAGLDTRPWRLDLPSS
                     LTWWDVDYPATHEYKREAMKDEGPKCRIEWAAADLADRGARKALFTRVGQSAKRVLVV
                     SEGLLIYLDPAEVRALATDLHEPASFANWLIDLASPKLLEWIQRRNMGKATAAGGAPM
                     KFGPAEGTAYFEPSGWREIEWHSTGEEARRLGREMSLAWLWRLMGVFSSAGTREKWRR
                     YAGYALLGRV"
     gene            2288..3115
                     /locus_tag="VFU75_02670"
     CDS             2288..3115
                     /locus_tag="VFU75_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465762.1"
                     /translation="MRSARASVVLLAALVASCDRTPPPPAAQTQANALRIVASDFSYV
                     SPDTVPAGLTNIVMVNQGKEPHQAAIFRMDSVKTPAEIQAGLHSNVIPSWVIVTGGPN
                     GANPGDSSNVAAVLQPGNYMMVCFMSGADGKMHLDKGMAKTFVVKPAGVPMALPTADI
                     TVTTKDYSFEISPAITAGTHTIRLVNVGPQLHEITLVKMAPGKTMKDAQAWMAAGMKG
                     MPPFMSFGGIAGLAVGQAANFTATFTPGDYVMLCFVPDVKDGKPHLAHGMVMPFKVS"
     gene            complement(3117..3740)
                     /locus_tag="VFU75_02675"
     CDS             complement(3117..3740)
                     /locus_tag="VFU75_02675"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEU5465763.1"
                     /translation="MSGRRPSLERERALWREGRMVVAGIDEVGRGPLAGPVVAAAVVF
                     YPDQKPIRGLNDSKVLTAKARDRLALEIRSRARAIGIGAASVHEIDRWNIRRAAILAM
                     RRALARLGLDPTDVLVDGMPCPELGRPHQAIVDGDAKCSSIAAASIIAKTVRDVLMAR
                     LAARHPGYAWESNKGYATADHLEAVARLGPTRHHRQSFSPVCQLALF"
     gene            complement(3737..4120)
                     /gene="rplS"
                     /locus_tag="VFU75_02680"
     CDS             complement(3737..4120)
                     /gene="rplS"
                     /locus_tag="VFU75_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010334229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEU5465764.1"
                     /translation="MERLRAVEREGLKTDIPAFDPGDTVRVMVRVREGEKERLQAFEG
                     VCIARRGGGINETFTVRKISAGVGVERIFPLHSPSISAIEMVRQGRVRRAKLYFLRRL
                     AGKAARIREKRGDTAGTQAAEQTQA"
     gene            complement(4148..4837)
                     /gene="trmD"
                     /locus_tag="VFU75_02685"
     CDS             complement(4148..4837)
                     /gene="trmD"
                     /locus_tag="VFU75_02685"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEU5465765.1"
                     /translation="MLTVSLVTLFPEMFTGPLGASILGRAAKSGLVRYRVVQLRDYTH
                     DRHRTVDDTPYGGGAGMVLRPEPFFEALDDLKPAAGSPVVLMSARGKPLTHDDAVRWS
                     LAAELTVLCGHYKDVDQRVADGLATEEVSVGDAVLTGGEPAALCLVDAVVRLLPGALG
                     DHESAAGDSHYDGLLAPPSYTRPPEYRGMAVPPVLIGGDHAKIAAWRAAEAERLTRER
                     RPDLWEKHQGA"
     gene            complement(4841..5185)
                     /locus_tag="VFU75_02690"
     CDS             complement(4841..5185)
                     /locus_tag="VFU75_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465766.1"
                     /translation="MAREGRDADRYGALPAEESGSVRPTPRVIARLRKPHGQKGEVAI
                     FPLVADPGSLLVPKARMYVVDETRQVLAGPLILGRRRAYHREWLVGFEGVSSRAAVEE
                     WRDQFLAVEDEG"
     gene            complement(5101..5358)
                     /gene="rpsP"
                     /locus_tag="VFU75_02695"
     CDS             complement(5101..5358)
                     /gene="rpsP"
                     /locus_tag="VFU75_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEU5465767.1"
                     /translation="MATRIRLRRMGRKGQAHFRIVVADQESPRDGRFVATLGHYNPKT
                     KPTTLQVDAERAKYWLGKGATPTDTVRSLLKKAGVFGQRPA"
     gene            complement(5367..>6025)
                     /locus_tag="VFU75_02700"
     CDS             complement(5367..>6025)
                     /locus_tag="VFU75_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEU5465768.1"
                     /translation="VKPKDVLLVADGMTGQDAVKIAKGFHESVGLTGAILTKMDGDSR
                     GGAALSIHGVTGVPIVYVGVGEKVEALEPFDPVRMAGRILGQGDVVGLVEKAAAAIDL
                     KAAQKLEQKARSKRGMDLADFLVALKQMQAMGPMKQVLGLLPGVNAKALKQIPADDKR
                     LKHVEAIVLSMTPGERADPSVLNGSRKLRIAKGSGTSVQEVNRLLEQFQQMRKLIKRM
                     "
BASE COUNT          965 a         2115 c         1945 g         1000 t
ORIGIN      
        1 ctgaagaagg acggcacgcc gctgtggtcc ctgctgacga catccctggt gcggacggcg
       61 gatggcggac cggcgttctt catcaccgtc atcgaggacc tgtccgaacg gaagcggctt
      121 gaagcgcagc ttctgcacgc gcagaagctg gaggccgtcg ggcagctcgc cggcggcgtg
      181 gcgcacgact tctcgaacct gatcggcgcc atccaggggt acgcgcagct ggtcgagcgc
      241 accctggcac ccgaggaccc ccggcgagcc gacatcggtg aaatccggaa ggcgggacag
      301 cgcgccgcgg gcctcattca tcagttgctg accttcgccc gccggcagga gacgcagccg
      361 cagctggtcg acgtgaacgg cctggtcacc aacctcggtc gcctggtgag ccgcgtcctc
      421 ggctccaaca tcaaggtgtc gtggggtctg aacgaagacc tgcccgcgat caaggtcgat
      481 ccgggtcagt tcgaacaggt gatactgaac ctggccatca acgcccggga cgcgatgccg
      541 gatggcggaa ccctcggcat cgagacgtca ctcgtgcagg tggatcagag tcacgtgaac
      601 cgccagcgcg gctcccggcc cgggcctcat gtgatggtga ccgtctccga cacggggacc
      661 ggcatgcctc gggaggtgct ggaccggatc ttcgagccct tcttcacgac caagggtccc
      721 gatcgcggca ccggcctcgg cctcgccatc tgctacggga tcgtgaagca ggccggtggc
      781 aacatctggg tgtactcgga acccggacac ggaacgacgt tccgcctcta tttccccgcc
      841 gtcctcgagg gcgccggcgc catgtccctc cccgaatcgg accccgagct gcccggcggg
      901 agcgaacgcc tgttgctggt ggaggacgat cctcagatgc gggcgctcgc gctccgggtc
      961 ctgtccgact gcggctatga cgtcaccgcg acgccggacg ctgaaacggc gatggcggag
     1021 gtggcgtcgg gcgggctcga cccggccctg ctggtcaccg atctcattct ccccggcgtc
     1081 accggattgc agatggctca ggccctgcgc gccgaaaaac ccgggctccg cgtgctgtgc
     1141 atgacgggac aggcggatcg ggtctcgtcg tcgagccggc ggcagcaggg gtttgggttg
     1201 atcgagaagc ccttcacggc gcaggcgttg gcgcgggcgg tgcgggacgc gctggaccag
     1261 cagccgtcag ccatcagcga tcagccgtca gcgaattgaa tacgccagcc tgctgacggc
     1321 tgacggcttg gcatcctaga ttcccactca tgcctgagac tccgttggtc tcccacatct
     1381 ccgacaccgc ccgttgggtg gcgatgtacc gcgccttcga gtcggaacgc cccgacgcga
     1441 tcttccacga cccctacgcc cgcaggctcg cggggccggt cggcgagcgg atcgccgccg
     1501 cgatgggtgg aacgaagtgg gcgtggccca tgattgtgcg caccgccgtg atggacgagg
     1561 tgatccagcg ggtggtgggc gaaggcgccg acaccgtcct caatctggcc gcgggtctcg
     1621 acaccagacc ctggcgcctc gacctcccgt cgtcgctcac gtggtgggac gtcgactacc
     1681 cggccacgca tgagtacaaa cgggaagcga tgaaagacga ggggcccaag tgccggatcg
     1741 agtgggccgc cgcggacctt gccgaccgtg gcgcccggaa ggcgctcttc acacgcgtgg
     1801 gccagtcggc gaagcgggtg ctcgtcgtgt cagaaggcct gctgatctat ttggatccgg
     1861 ctgaggtacg cgcgctggcg accgatctcc acgagccggc gtcgttcgcg aactggctca
     1921 tcgacctcgc gtccccgaag ctcctcgagt ggatccagcg ccggaacatg gggaaggcga
     1981 cggccgccgg gggcgccccg atgaagttcg gtccggccga aggcacggcg tacttcgaac
     2041 ccagtggctg gcgggaaatc gagtggcatt cgacgggcga ggaggcccga cgcctgggcc
     2101 gggaaatgtc gctggcgtgg ttgtggcgtc tgatgggggt attttcgtcg gccgggacgc
     2161 gcgagaaatg gcgccgctac gcgggttacg cgttgctcgg gagggtgtga agttcgtctc
     2221 ggtttgcgac gccgccttcc gaccgtagat tctcggctcc ccatctctcc tcaactgagg
     2281 tccccaaatg cgttcagctc gagcttccgt cgtgctcctt gccgcgctcg tggcgtcctg
     2341 tgaccgtaca ccgccaccgc ccgccgcgca gactcaggcg aatgccctcc ggatcgtcgc
     2401 gagcgacttt tcgtacgtga gcccggacac ggtgccggct ggactcacca acatcgtgat
     2461 ggtgaatcag gggaaggagc cccaccaggc cgcgatcttc cggatggact cggtcaagac
     2521 gcccgccgaa atccaggctg gactgcattc caacgtcatc ccgagctggg tcatcgtcac
     2581 cggtggcccg aacggcgcca atcccggcga ctcgagcaac gtggcggcgg tcctgcagcc
     2641 cgggaactac atgatggtct gcttcatgtc gggcgcggac ggcaagatgc atctcgacaa
     2701 gggcatggcc aagacgttcg tcgtgaagcc cgccggcgtc ccgatggcgc tgccgaccgc
     2761 cgacatcacg gtcaccacca aggactattc attcgagatc tcccccgcca tcacggccgg
     2821 gacgcacacc atccgtctcg tgaatgtcgg cccgcagctg catgagatca ccttggtcaa
     2881 gatggcgccg ggaaagacga tgaaggatgc gcaggcctgg atggcggcgg gcatgaaggg
     2941 catgccgccg ttcatgtcct tcggcggcat cgccggactc gcggtaggtc aggccgcgaa
     3001 cttcacggcc acgtttactc ctggcgacta cgtcatgctc tgctttgtac ccgacgtcaa
     3061 agacgggaag ccgcacctcg cgcacggcat ggtgatgccg ttcaaggtaa gctgaactag
     3121 aagagggcga gctggcacac cggcgaaaag ctctgccggt ggtgcctggt aggcccaagc
     3181 ctcgcgacgg cttcgagatg atcggcggtc gcgtaaccct tgttcgattc ccacgcgtac
     3241 cccggatggc gcgccgcaag gcgcgccatc agcacatcac ggaccgtctt ggcgatgatc
     3301 gaggccgcgg cgatggacga gcatttggcg tccccatcca cgatggcctg atggggacga
     3361 ccgagttccg gacagggcat gccgtccacc aggacgtcgg tgggatccag gccgaggcgc
     3421 gcgagggcgc ggcgcatcgc gaggatggcg gcgcggcgga tgttccagcg gtcgatctcg
     3481 tggacgctcg ctgcgccgat gccgatcgca cgcgcgcggg aacgtatttc gagggcgagg
     3541 cggtcgcggg ccttggcggt gaggaccttc gagtcgttca gcccgcgaat gggcttctgg
     3601 tccggataaa agacgacggc cgcggccacc accggtcctg ccagggggcc acggccgact
     3661 tcatcgatgc cggcgacgac catccgcccc tcgcgccaga gcgcgcgttc gcgctcgagc
     3721 gaggggcggc ggccgctcac gcctgggtct gctccgccgc ctgagtcccg gccgtatcgc
     3781 cgcgcttctc gcggatccgg gcggccttgc cggccaaccg gcgcaggaag tacagcttgg
     3841 cccggcgcac gcggccctgg cggaccatct cgatggcgga aatggacggc gagtgcagcg
     3901 ggaagatgcg ctcgacgccg acacccgcgg agattttccg gaccgtgaag gtctcgttga
     3961 tcccgccgcc gcgccgggcg atgcacacgc cctcgaacgc ctgcagacgc tccttttcac
     4021 cttcacgcac gcgcaccatc acccggaccg tatcgccggg gtcgaaggcc gggatgtccg
     4081 tcttcaggcc ttcacgctcc accgctctga gtcgctccat aactgctccc tgtttctgta
     4141 gggcaaccta cgcgccctga tgtttctccc acaagtccgg ccgtcgttcc cgcgtcagcc
     4201 gttcggcctc ggcagcccgc catgccgcga ttttcgcatg atctccgccg atcagaaccg
     4261 gcgggaccgc cattccccgg tactccggag gccgcgtgta cgacggcggc gccagcaggc
     4321 cgtcgtagtg cgaatccccc gccgcggact cgtgatcgcc cagggcgccc ggcaacagcc
     4381 ggaccaccgc gtccaccagg cagagcgccg ccggctcacc cccggtcagc accgcatcgc
     4441 cgaccgacac ttcctcggtg gccaagccgt cggccacccg ctgatccacg tccttgtagt
     4501 gcccgcagag caccgtcaac tcggccgcca gcgaccaccg caccgcgtca tcgtgcgtca
     4561 ggggtttccc ccgcgccgac atcagcacca ccggactgcc ggccgccggc ttcaggtcgt
     4621 cgagcgcctc gaagaacggc tcaggcctca gcaccatgcc cgcgccgccg ccgtacggcg
     4681 tgtcgtccac ggtgcgatgc cggtcgtgcg tatagtcccg cagctgcacc acccggtagc
     4741 gcaccaggcc tgacttcgcc gcgcgaccga ggatactcgc gccaaggggc ccggtgaaca
     4801 tctcgggaaa gagggtgacg agactgaccg tcagcatccc ttatccctcg tcctccacgg
     4861 ccaggaactg atcgcgccac tcttccaccg cggcgcggct gctcacgcct tcgaatccca
     4921 ccagccattc acggtgatag gcccggcgcc ggcccaggat cagcggaccg gccagcacct
     4981 gccgcgtttc atccaccacg tacatccgcg ccttcggcac cagcaggctt ccgggatccg
     5041 ccaccagcgg gaagatggcc acctcccctt tctggccgtg cggcttccgg agccgcgcga
     5101 ttacgcgggg cgttggccga acactcccgc tttcttcagc agggagcgca ccgtatcggt
     5161 cggcgtcgcg cccttcccga gccaatactt ggcccgttcc gcgtccacct gaagggtggt
     5221 gggcttggtc ttggggttgt agtgccccag cgtcgccacg aaccggccgt cgcgcggaga
     5281 ctcctgatcc gccaccacga tccggaagtg cgcctggccc ttccgtccca tccgccgcaa
     5341 tctgattcga gtcgccatac agttccttac atcctcttga tgagcttgcg catctgctgg
     5401 aactgttcca gcagacggtt gacttcctgc accgacgtgc ccgacccctt cgcgatccgc
     5461 agcttgcgcg atccgttcag caccgacgga tcggcccgtt cgcccggggt catcgacagc
     5521 acgatcgctt ccacatgctt caaccgcttg tcgtcggcgg ggatctgttt cagcgccttg
     5581 gcattcacgc ccggcaacag tcccaggacc tgcttcatcg gtcccatcgc ctgcatctgc
     5641 ttcagcgcca ccaggaaatc cgcgaggtcc atcccccgct tggagcgcgc cttctgctcg
     5701 agcttctgcg ccgccttcag gtcgatcgcg gcggcggcct tttcgaccag ccccaccacg
     5761 tcgccctggc ccaggatccg gcccgccatc cgcacgggat cgaatggttc gagcgcttcg
     5821 accttttcgc cgacgccgac gtacacgatc ggcacgcccg tcacgccgtg aatcgagagc
     5881 gccgcgccgc cgcgggagtc gccgtccatc ttggtgagaa tcgcgccggt caggcccacg
     5941 ctctcgtgga atcccttggc aatcttcacc gcgtcctggc cggtcatgcc gtcggccacc
     6001 agcaggacgt ccttcggctt cactg
//
LOCUS       DASUBP010000064         2184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_669364, whole genome shotgun sequence.
ACCESSION   DASUBP010000064 DASUBP010000000
VERSION     DASUBP010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669364"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1443
                     /locus_tag="VFU75_02705"
     CDS             <1..1443
                     /locus_tag="VFU75_02705"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HEU5465769.1"
                     /translation="PGDVLVLDSPWATNYNTFVMNRLQFTDPEAMIKHVHDQGFKLCL
                     WLTAFINKESIKAPEPELVGKIPETEASNYEEAAKNGYFLKDSTGGVYLATWWKGKGG
                     LIDFTQPAAVRWWQNQVRQAVAIGADAFKADGGEGSFIAYPESLRTRYSVLYNQALEH
                     VIDTDLHGDGVLFSRSGSVGSHDLPFIWAGDNQADFSKDNGLPSVVTAGLNAGLSGVS
                     MWTSDLGGYEKTARTPGDDTVFVRWTEFSAFSPIMEVHSSINLGPWDYGDEALDIFRR
                     YSRLHMSLFPYRYRAAQESHRTGMPLMRALVFEFSTDSAARTATDEYEFGPDLLVAPM
                     VTAETSRTIHIPRGEWIDYWSGAPVSGPKDTTVAVPLDRIPLFVRAGAILPKIPEDVM
                     TLVPKLDDRRIYEIYPGPGRTLMDFEGRRLDRAGNKLRIGGKAAQVTLTWRGERPRGV
                     RVNGKPVALNADSVSVTFSHQGETVVAWEP"
     gene            complement(1474..>2184)
                     /locus_tag="VFU75_02710"
     CDS             complement(1474..>2184)
                     /locus_tag="VFU75_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465770.1"
                     /translation="LINFDRRQLVYPLTERFIGFGQETEDVALPKSLGLPLDHGQRIG
                     LYLMWDNEGANALTGVQVRLTFHWAPPNQLPPVIPVMPLIVDAHLVAGGTDGFSVPPG
                     GSVTSVDFSLPISGHLLAATGHLHDQGLWVRVEDPATGAVIVTVPARRDSTGRVLGMS
                     RRLLAVWGNGPHLEAGRRYRLSVAYANPTSDTLRGAMGFLYGAFVPDRLSDWPAIDRT
                     NADYLEDLKSLEPKHSGE"
BASE COUNT          389 a          681 c          744 g          370 t
ORIGIN      
        1 cccggcgacg tgctggtact cgacagtccc tgggccacca actacaacac cttcgtgatg
       61 aatcgcctcc agttcacgga tcctgaggcg atgatcaagc acgtccacga ccagggcttc
      121 aagctctgtc tctggctcac ggccttcatc aataaggagt cgatcaaggc gcctgagccc
      181 gagttggtcg gaaagatccc ggagaccgag gcatcgaatt acgaggaggc cgcgaagaac
      241 ggctacttcc tgaaggattc gacgggcggt gtgtacctcg cgacctggtg gaaggggaag
      301 ggtgggctga tcgacttcac ccagccggct gccgtgcgct ggtggcagaa tcaggtgcgc
      361 caggcggtcg ccatcggtgc cgacgcgttc aaggccgacg gaggggaggg cagcttcatc
      421 gcgtaccccg agtccctccg cacgcggtat tccgtgctct acaaccaggc actggagcac
      481 gtgatcgata cggacctcca tggcgacggc gtgctcttca gccgctcagg atccgtgggc
      541 agtcatgacc tcccgttcat ctgggcgggc gacaatcagg ccgacttcag caaggacaac
      601 ggcctgccga gtgtggtgac ggcggggttg aacgcggggc tgtccggcgt gtcgatgtgg
      661 accagcgacc tcggcgggta tgagaagacc gcgcggacac ccggtgacga cacggtgttc
      721 gtccgctgga ccgagttctc cgccttctcg cccatcatgg aagtgcactc gagcatcaac
      781 ctcggcccct gggactacgg ggatgaggcc ttggacattt tccgccgata ctcgcgcctt
      841 cacatgagcc tgttcccgta ccggtatcgg gcggcgcaag agagccaccg gaccgggatg
      901 ccgttgatgc gggccctcgt attcgaattc tccaccgatt cggccgcccg gaccgccacc
      961 gatgagtacg agttcgggcc cgatctcctg gtggctccga tggtgaccgc cgagacgagc
     1021 cgcacgattc acatcccccg tggtgaatgg atcgactact ggtcgggggc acccgtgtcg
     1081 ggtcccaagg acacgacggt cgccgtaccg ctcgaccgga ttcctctctt cgtgcgcgcg
     1141 ggtgcgatct tgccgaagat cccggaagac gtcatgacgc tggtgcccaa actcgatgac
     1201 cggcggatct acgagatcta tccgggaccg gggcggaccc tgatggattt tgaagggagg
     1261 cggctcgatc gcgcgggaaa caaactccgc atcgggggca aggcggcgca ggtgacgttg
     1321 acttggcgtg gcgagcgtcc gcggggggtc cgcgtcaatg ggaaaccagt tgccctcaac
     1381 gccgactcgg tgtccgtgac gttctcccac cagggagaga cggttgtggc gtgggagccc
     1441 tagcttggca atgctcccat catgcgcacg ggatcactcc ccggagtgct tgggttcgag
     1501 actcttcagg tcttccaagt aatcggcgtt ggtgcggtcg atcgcgggcc agtccgacaa
     1561 ccggtcgggg acgaacgcgc cgtacagaaa tcccatggcg ccccgcaggg tgtccgaggt
     1621 cggattggcg tatgcgacgg agagccgata ccggcgtccc gcctcgaggt gaggcccgtt
     1681 cccccacacg gccagcaggc gacgcgacat tcccaacacc cggcccgtcg agtcccgtcg
     1741 cgcggggacg gtgacgatca ccgcaccggt ggccgggtct tcgacacgga cccagagccc
     1801 ctggtcgtgg aggtgcccgg tggcggcgag gaggtggccg ctgatgggca gggagaagtc
     1861 gacgctggtc acgctgcctc cgggaggcac cgagaagcca tcagtgcccc cggccacgag
     1921 atgggcgtcg acgatgagcg gcatgacggg aatcaccggg ggcagctggt tcgggggcgc
     1981 ccagtggaag gtcagccgca cctgcacgcc cgtaagcgca ttcgctcctt cgttgtccca
     2041 catcaggtag agcccgatcc gctggccgtg atcgaggggg agccccaggg acttggggag
     2101 cgcgacgtcc tccgtctcct ggccgaatcc gatgaaccgt tcggtgaggg ggtacacgag
     2161 ctgccggcga tcgaagttga tgag
//
LOCUS       DASUBP010000065         4186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_273973, whole genome shotgun sequence.
ACCESSION   DASUBP010000065 DASUBP010000000
VERSION     DASUBP010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4186
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_273973"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /locus_tag="VFU75_02715"
     CDS             <1..1140
                     /locus_tag="VFU75_02715"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5465771.1"
                     /translation="PDLAQLQELSERSIAAMKPVPGLVELKSSLEGRKPEFVIDVNRD
                     LAANVGLSVGAIGQSLRPILAGQTAGTWEDETGLAHDVMVRLAPEYRRSEVDVARIPV
                     ASSQTNAKTHTPVMVPLGQVATLASSGAPSEIKRLNLERVVEIEGNYQTRPLTAVMQD
                     AQAKLAQVPLPAGYRWDVGGEQKDFAETVGYILESLFLAVVFIYLILASQFGSFLQPF
                     AIMLSLPLSLIGVMLGLMVTHGTFNIMSMIGVIMLMGLVTKNAILLIDFVNQAREKGS
                     DRRKALIDAGELRLRPIVMTTLAMIFGMLPTALALGAGSEFRAPMAVAVIGGLITSTL
                     LTLVVVPVVYTYLDDFGGWVGAWVKGWTSAPESVHAPKAAAAPGD"
     gene            1150..2808
                     /locus_tag="VFU75_02720"
     CDS             1150..2808
                     /locus_tag="VFU75_02720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HEU5465772.1"
                     /translation="MTPSPEREQTRRWVVLAIVAGAAFRLVHYGLNPSLTLDDAMLSF
                     NLASRSWAGLAHPLAFQQTAPIGFLWASRAATLLGGLNEFALRAVPVLAGVATPYGVW
                     LLARRLVSAGAAVVAAFFAALSPILIQYSISVKPYETDALFAVGVALAALTTLDRPVR
                     RNWAALGGVGVAALLFSTPAVFVLAGCGLALALTLRRRALLPLTLVGTAWIALFAVLY
                     WTVTRAEAVSPYMQWFWDHKFLTPAVVLSEPGRAWDIVQRLPTQVFTGDAPQLIALIL
                     CWFAAVAGVWHISRTQGARVWVLLGPIAFALVASMFRRYPVAPRLFLFAAPLVAVILA
                     AGVERGRMHLAAGAGALRLGFEALVVLWLITIGVLSANTSRLWAPPTRPLIQELRRQR
                     VDHEPLYVFSGAAPAWLLYATHWDDPSDTSFAMTVAAAERWDGNAFQNAASRGHAVAD
                     TEGGRLIGVQGGFPVIIGLSTGIAWREGQWLSQLQPDSGWAEREAARMDAVTDSTAWM
                     LFSQLARREVPLLVHALATRHGQVILEQGSRGAFLYHIRFLRTR"
     gene            2805..>4186
                     /locus_tag="VFU75_02725"
     CDS             2805..>4186
                     /locus_tag="VFU75_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465773.1"
                     /translation="MNRPRLVLLLVWLLSVGYAASGLTRGWWPHDEGTLGQSADRVLA
                     GQLPHRDYDEIYTGGLAFVDAAAFKVFGDTLVSPRLVLLLVFAIWVPAMFYVASRFFG
                     PVGAGGVTLAAAAWSIPNYSAAMPSWFNLFFAGLGVACLVRHTETGAARWIFWAGICG
                     GLSCLCKIIGLYYIAGALLFLVYREQSAANGASEPGALWFRVVVGSALLVFALGVLDL
                     LRGRWTGGDLGLYAFPSAALVALLLAGEPGRGSGRARFSALARSVGILAAGVALPVVL
                     FLIPYILSHSVGAIAYDVFVAPTRRFMFAAMRAPGLNTFRNALLFIIPVGLYWWLPTW
                     RRGIAWVTAILLAAVWVLTFKGNYYRIEWNAFVWIVPLAVAAGAIILWPRTGGGPPTV
                     KRQQTFALLAVTAVATLVQFPFSAPIYFLYIAPLGVVTAAAVLEIQFEGAAAWPVFGG
                     ALLLFAILFPV"
BASE COUNT          554 a         1369 c         1418 g          845 t
ORIGIN      
        1 cctgatctcg cccagcttca ggagctttcg gagcgttcga tcgcggcgat gaagcccgtg
       61 ccgggactgg tcgagctcaa gtcgtccctc gaagggcgga agcccgaatt cgtcatcgac
      121 gtcaaccgcg atctcgcggc gaacgtcgga ctgtcggtgg gcgccatcgg gcaatcgctc
      181 cggccgattc tggccgggca aacggcgggc acctgggagg atgagaccgg tctggcgcac
      241 gacgtcatgg tgcggctggc acccgaatac cgccggtccg aagtggatgt cgcgcgcatt
      301 ccggtggcgt ccagccagac caatgcgaag acgcacacgc ccgtcatggt gccccttgga
      361 caggtcgcga ccctcgcgtc cagcggcgcg ccgagtgaga tcaaacgcct gaacctcgag
      421 cgcgtcgtgg agatcgaggg caattaccag acccgcccgc tcaccgcggt gatgcaggac
      481 gcccaggcca aactcgcaca ggtgcccctg cccgccggct accggtggga cgtgggcggg
      541 gagcagaagg acttcgcgga gacggtgggc tatatcctgg agtcgctctt cctggccgtg
      601 gtcttcatct atctgattct ggcgagccag ttcgggagct tcctgcagcc gttcgcgatc
      661 atgttgtcgc tcccgctctc cctcatcggt gtgatgctgg gtctgatggt gacgcacggg
      721 acgttcaaca tcatgagcat gatcggcgtg atcatgctga tgggattggt gacgaagaac
      781 gccatcttgc tgatcgactt cgtcaaccag gctcgcgaga agggcagcga ccggcggaag
      841 gcgctgatcg acgccgggga gctgcggctt cggcccatcg tgatgaccac cctggccatg
      901 atcttcggca tgttgccgac ggcactggcg ctgggcgcgg gctccgagtt ccgggcgccc
      961 atggccgtgg cggtaatcgg cggcctgatc acgtcaacgc tcctgaccct cgtggtggtc
     1021 ccggtcgtgt acacgtacct ggacgacttc ggtggttggg tcggagcatg ggtgaagggt
     1081 tggacgagtg cccccgaatc cgttcacgcc ccgaaggcgg cggcggctcc gggagactaa
     1141 tttcacgggg tgacgccctc ccccgagcgc gagcagacga ggcggtgggt ggtgctggcg
     1201 atcgtcgccg gcgccgcctt ccgcctcgtt cattacgggc tgaacccttc cctcaccctc
     1261 gacgacgcga tgttgtcgtt caacctcgcg tcccgttcct gggccggcct tgcgcacccc
     1321 ctcgcattcc agcagaccgc gccgattggc ttcctgtggg catcacgggc ggccacactc
     1381 ctgggcggct tgaacgagtt tgccctccgg gcggtgcccg tactcgccgg agtggcgacc
     1441 ccgtacggcg tctggctact cgcgcggcga ctggtgagtg cgggagccgc cgtagtcgcc
     1501 gccttcttcg ccgcgctctc gcccattctc atccagtact ccatctccgt gaagccgtac
     1561 gagactgacg cgcttttcgc cgtaggggtc gccctcgcgg ccctcacgac cctcgaccgg
     1621 ccggtgcgcc ggaactgggc cgcgctcggc ggcgtcggcg tcgcggcgct cctgttctcg
     1681 accccagcgg tcttcgtcct ggctggttgc ggtctcgcgc tggccctgac cctccgtcgc
     1741 cgggcgttgc ttcctctcac gctggttgga accgcctgga tcgcgctctt cgccgtgttg
     1801 tactggaccg tcacccgggc cgaggcggtg agtccctaca tgcaatggtt ctgggaccac
     1861 aagttcctca cccccgcagt cgtcctctcg gagcccggga gggcatggga tatcgtgcag
     1921 cgccttccca ctcaggtgtt caccggtgac gcgccacagc tcatcgcgtt gatcctgtgc
     1981 tggttcgcgg cggtggccgg cgtatggcac atctcccgca cgcagggcgc gcgcgtgtgg
     2041 gtactgctcg ggcccatcgc gttcgccctg gttgcctcca tgttccgtcg ctatcctgtg
     2101 gcgccgcgcc tgtttctgtt cgccgctcct cttgtggccg tgatactggc cgcgggcgtc
     2161 gagcggggaa gaatgcatct ggcggccgga gcgggggcgc tacgtctcgg gttcgaagcc
     2221 ctcgtggtcc tgtggctcat taccatcggg gtcttgagcg cgaacacgtc acgactctgg
     2281 gcgccaccca cccgtccatt gattcaggaa ctacggcgcc agcgggtgga ccatgagccc
     2341 ctctatgtgt tttccggggc ggctccggca tggcttctct acgccacgca ttgggatgat
     2401 ccgtcggaca cgtcttttgc gatgaccgtg gcggccgccg agcggtggga tggaaatgct
     2461 tttcagaacg cggcctcacg cggacatgcc gtggcggaca ccgagggcgg tcgactcatc
     2521 ggcgtacagg gtggatttcc ggtgatcatc gggctctcaa ccggcatcgc ctggcgggaa
     2581 gggcagtggc tgagtcaact ccagcctgat tccggctggg cggaaaggga ggctgcgcgg
     2641 atggatgcgg taacggattc gacggcatgg atgctcttca gccagctcgc gcgccgggaa
     2701 gtcccccttc tggtgcacgc gctcgccacg cgccacgggc aggtgatcct cgaacagggg
     2761 agccggggag cgttcctcta tcacatccgg tttttacgca ctcgatgaac cggcctcggt
     2821 tggtgctgct cctggtctgg ttgctgtcgg tcggctacgc ggcatccgga ctgacgcgcg
     2881 gttggtggcc tcacgacgaa ggcacgctgg gtcagagcgc ggatcgggtg ctggcgggcc
     2941 agctgccgca tcgggactac gacgagatct acaccggcgg gctcgcgttc gtggacgctg
     3001 cggccttcaa ggttttcggt gacacactcg tgtctccccg cctggtgctg ctcctcgtct
     3061 tcgcgatctg ggttcccgcg atgttctacg tggcctcccg gttcttcggc ccggtcggcg
     3121 ccggaggtgt cactctggcc gcggcggcat ggagcatccc gaattattcc gcggccatgc
     3181 cctcctggtt caacttgttc ttcgcggggt tgggcgtcgc ctgcctggtc cggcacacgg
     3241 aaaccggagc ggcgcgctgg attttctggg cgggcatctg cggcggcctg tcctgcctct
     3301 gcaagatcat cggactctac tacatcgcgg gtgcgttgct cttcctggtg taccgcgagc
     3361 agtcggcggc caacggagct tcggaacccg gtgcgctgtg gttccgcgtg gtggtcggga
     3421 gtgccctcct ggtgttcgca ctcggggtcc tcgatctgct ccgcggccgg tggactggcg
     3481 gcgatctcgg cctgtacgca ttcccatccg ccgctctggt ggccctgctg ctcgcgggcg
     3541 agccgggacg cggctccggt agagcccgtt tcagcgcgct ggcgcggagc gtcggcatcc
     3601 tggcggccgg cgtggccctc cccgtggtgc tcttcctgat cccgtacata ctctctcata
     3661 gcgtcggtgc gatcgcctac gacgtattcg tggcgccgac tcgccggttc atgtttgcgg
     3721 ccatgcgggc cccagggctc aacacgttcc ggaacgcgtt gctcttcatc attccggtgg
     3781 gactctactg gtggctcccg acctggagac gagggatcgc ctgggtgacc gcgatcctgc
     3841 tcgcggcggt gtgggtcctg accttcaagg ggaactatta cagaatcgag tggaacgcgt
     3901 tcgtgtggat cgtcccgctg gcggtcgccg ctggagcgat catcctgtgg ccccgcaccg
     3961 gcggtggtcc gccgactgtg aagagacagc agacgttcgc gctcctggcc gtgacggccg
     4021 tggcgacgct ggtgcaattt cccttcagtg ccccgatcta cttcctgtat atcgcgccgc
     4081 tcggcgtggt gacggccgcg gcggtactcg agatccagtt cgaaggggcg gcggcgtggc
     4141 cggtgttcgg gggcgcgttg ctgctgttcg ccatcctgtt cccggt
//
LOCUS       DASUBP010000066         2016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_996344, whole genome shotgun sequence.
ACCESSION   DASUBP010000066 DASUBP010000000
VERSION     DASUBP010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996344"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1407
                     /locus_tag="VFU75_02730"
     CDS             <1..1407
                     /locus_tag="VFU75_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU5465774.1"
                     /translation="DHYANSVVALRASTGKVAWSFQVVHHDLWDYDVPAQPVVFTLHK
                     NGHSYPALAVGTKMGHLFILDRRNGKPLVPVTERAVPQSNIPGEVTSPTQPFPPPAYR
                     FVPETLSSAQAFGIDAAGKAFCQAWMDSLRYQGIFTPASLQGSIHFPGHVGGFNWSGF
                     SIDEADGILVAPVNEIAMVVTLVPRDSLPAVRARHPDTEFGSQRGTPFASMRQNLLGR
                     NGPPCNAPPWGELVGFDLAHDRVKWRVPNGTIPGLEARQTGSPSLGGVLLTKGGLAFI
                     GATLDNHFRAYDISTGKVLWDVRLAAGGHALPATYSVGGRQYVVIAAGGHDRLHIGPP
                     LLGDYLYAFALDAPEVEDTAHDVAGPWTGDLRIEDHERFPMTFSLSPAGDSLTGEANV
                     GDGAITGPLSVHHAGTIVTWRLAFTYPAKSCGGEITGGGMEADQGRLLVGTLTVHSTC
                     SDHDEPGTFSFRPASARP"
     gene            complement(1382..1879)
                     /locus_tag="VFU75_02735"
     CDS             complement(1382..1879)
                     /locus_tag="VFU75_02735"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU5465775.1"
                     /translation="MRIVLIVVGVVVAVVVIVAVIGALLPVKHQVSREATFKISADKL
                     FALISTPADFPKWRTGVKSVEMLPSGGGLSKWTEVSSDGTITFAVTQAVPGVELVTTI
                     ADKSLPFGGSWTYVLAPAGPDSTTLRITEDGEVYNVIFRFMSRFVFGHAGTIEKYLKD
                     VQKRV"
     gene            complement(1864..>2016)
                     /locus_tag="VFU75_02740"
     CDS             complement(1864..>2016)
                     /locus_tag="VFU75_02740"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_084140945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEU5465776.1"
                     /translation="ANGRNPVAIVVPCHRVIGANGDLTGFGGGMARKEWLLKHEGGIL
                     TLCASS"
BASE COUNT          328 a          697 c          646 g          345 t
ORIGIN      
        1 gatcactacg ccaactcggt ggtcgccctt cgggcgtcga ccgggaaagt cgcctggtcg
       61 tttcaggtgg tgcatcacga cttgtgggac tacgacgtgc cggcacagcc ggtggtcttc
      121 acgctccaca agaatgggca ctcgtacccc gcgctcgcgg tgggcacgaa gatggggcac
      181 ctgttcatcc tcgaccggcg gaacgggaaa ccgctggttc ccgtgaccga gcgtgcggtg
      241 ccccaatcga acatcccagg ggaagtcacg tccccgacgc agccgttccc gccccccgcc
      301 taccgcttcg ttccggagac cctgtcatcg gcgcaagctt tcgggatcga tgcggcgggc
      361 aaggcgttct gccaggcctg gatggactcg ctccgctacc agggaatctt cacgcccgca
      421 tcgctccagg gctccatcca tttccccggc cacgtcggcg gtttcaactg gtcggggttc
      481 tcgatcgatg aggccgatgg catcctcgtc gcgcccgtga acgaaatcgc gatggtcgtg
      541 accctggttc cccgcgattc actcccggcg gtccgcgcga ggcacccgga tacggagttc
      601 ggttctcagc gcgggacacc gttcgcgtcc atgcgccaga atctgctcgg ccggaacggc
      661 ccgccgtgca acgcgccccc ctggggcgag ctcgtcggat tcgatctcgc gcatgaccgc
      721 gtcaagtggc gtgtgcccaa cgggaccatc cccggcctcg aggcacggca gacgggctcc
      781 ccttcgctcg gcggagtgtt gttgaccaag ggcgggctcg cgttcatcgg cgcgacgttg
      841 gacaaccact tccgcgccta cgacatctcc acggggaagg tcctgtggga cgttcgtctc
      901 gccgccggag gccacgcgct gccggcgacg tattcagtcg ggggccgcca atacgtcgtc
      961 atcgccgccg ggggccacga ccggctgcac atcggcccgc ccctgctcgg cgattacctc
     1021 tacgcctttg ccctcgatgc tccggaggtc gaggacacgg cgcacgacgt ggcgggtccc
     1081 tggacggggg acctgcggat tgaagaccac gagcgttttc cgatgacctt ctcactctcg
     1141 cccgcgggtg actcactcac cggggaggcg aacgtcgggg atggcgcaat caccgggcca
     1201 ctcagcgtcc accatgcggg gacgatcgtg acctggcgct tggctttcac ctatccggcc
     1261 aagtcgtgcg gtggagaaat cacaggcggg ggaatggaag ccgatcaggg aaggctgctg
     1321 gtcggcaccc tgaccgtgca ctcgacctgc tcggaccacg acgaaccggg gaccttctcg
     1381 ttcagacccg cttctgcacg tccttgagat acttctcgat ggtaccggcg tggccgaaga
     1441 cgaagcggga catgaagcgg aagatgacgt tgtagacctc gccgtcctcc gtgatccgca
     1501 gcgtggtcga atcggggccc gccggcgcga ggacgtaggt ccagctcccg ccaaagggca
     1561 ggctcttgtc ggcgatggtg gtgaccagct ccacgccggg caccgcctgc gtcaccgcga
     1621 acgtgatggt gccgtcggag gagacctctg tccacttcga caggcccccg cccgatggca
     1681 gcatctcgac gctcttcacg ccggtgcgcc atttgggaaa gtcggccggc gtgctgatca
     1741 gcgcgaacag cttgtccgcg gagatcttga aggtcgcctc ccggctcacc tggtgcttca
     1801 ccgggagcag ggctccgatg accgccacga tgaccaccac cgcgacgacg accccgacga
     1861 cgattaggac gatgcgcata gagtcaagat ccccccttcg tgcttgagca gccactcctt
     1921 ccgggccatc ccgccgccga agccggtcag gtccccattc gcgccgatca cgcggtggca
     1981 gggaaccacg atggccacgg gattccgccc gttcgc
//
LOCUS       DASUBP010000067         2988 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1268727, whole genome shotgun sequence.
ACCESSION   DASUBP010000067 DASUBP010000000
VERSION     DASUBP010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1268727"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..219
                     /locus_tag="VFU75_02745"
     CDS             <1..219
                     /locus_tag="VFU75_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465777.1"
                     /translation="ATFTMGMALVVLGRGIRVGKVFGRWLAETGTATGLINVAVAVLF
                     SETSQVMYVGQGVIIAWLAAVAVMMLAF"
     gene            complement(216..1808)
                     /locus_tag="VFU75_02750"
     CDS             complement(216..1808)
                     /locus_tag="VFU75_02750"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU5465778.1"
                     /translation="MSARTRDWLKFVSLVGVAFVFGLLFASALNLTPKSTAAVLPQLS
                     TAAQTQVPQAKSAAQIGDAFVAISNRVRPAVVYVSSARTERQDNSQVPAPFRDFFQIP
                     GQPRHPQVEEGSGTGFIVSADGYILTNNHVVAGADRVNVKLFDKREFKNVRVVGTDPQ
                     TDVAVLKIDALNLPTVTLGNSDSTNIGEWVLAIGNPLGEEFDFTVTAGIVSAKGRPLL
                     GLARGGLQIQDFIQTDAAINPGNSGGPLVNIKGQVIGINSAIASGTGYYTGYGFAIPI
                     NLVHTVFDQLVASGHVTRSVIGVTVTDAGEADAKAVGMPDDNIHGVVVDDYTPGGESP
                     AKDAGIEQGDVITALDGQPVNYTAQLQQIIGFKKPGDRVAVTLWRQGVGQKTISVKVI
                     SASSDMDKQLARSTKGDAGGSDEPAATPRTGGRVLGIGVQPLTPDDLGQDSRLARVSD
                     FGGGMAVTSVAPEGPAYGLIFPQDESGQLFIVMNVNGKPTRNWEEYRAAIKDLKHGQV
                     VTLRVFILGRDAGAEKIIRLEI"
     gene            complement(1837..>2988)
                     /locus_tag="VFU75_02755"
     CDS             complement(1837..>2988)
                     /locus_tag="VFU75_02755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEU5465779.1"
                     /translation="EKAKMELSSAMQTEINLPFITADQSGPKHLNLSMTRAKLEQLVD
                     TLVQRTIPPMEQALKDAGLKPSEIDEVILVGGQTRMPKVQEVVQKFFGKEPHKGVNPD
                     EVVAIGAAIQGGVLAGEVKDVLLLDVTPLSLGIETLGGVTTVLIPRNTTIPTKKSEVF
                     STAEDTQTTVEIHVLQGERQMARDNRTIGKFQLTGIPPAPRGVPQIEVTFDIDANGIL
                     HVSAKDKATGKEQKIRIEASSGLGDKDIDKMVKEAEAHAAEDKDRREEIERRNRLDAM
                     VYEVEKNSKEWADKLEQGLKTRLDQAVDGAKQALRAGDAAGIGKALEELQQAYSAAGA
                     SLYAQSRGADPSSAPGAGPEAQPNAGTEAKQENVVDADYEIVDDDKNKK"
BASE COUNT          435 a          997 c          956 g          600 t
ORIGIN      
        1 gcgacgttca cgatgggaat ggcgctggtg gtcttggggc gggggatccg ggtcgggaag
       61 gtattcggac gctggctcgc ggaaacagga accgccacgg gactgatcaa cgtcgccgtg
      121 gcggttctct tttccgaaac ctctcaggtc atgtacgtcg gccagggcgt catcatcgcc
      181 tggctggccg ctgtggccgt gatgatgctg gccttctaga tctcgagccg gatgattttc
      241 tcggcgcctg cgtcccggcc gaggatgaag acccgcagcg tcaccacctg accgtgcttc
      301 aggtccttga tcgccgcacg atactcttcc cagttccgcg tcggcttgcc gttcacgttc
      361 atgacgatga acagctgacc actctcatct tgcgggaaga tcaggccgta ggccggacct
      421 tcgggagcca cgctggtcac ggccatcccg ccgccgaaat ccgatacccg ggcgagccgg
      481 ctgtcctggc ccaggtcgtc gggtgtgagg ggctggacac cgatccccag cacccggcca
      541 ccggtccgcg gcgtcgcggc cggttcatcg gaccccccgg cgtcaccctt ggtgctccgc
      601 gccagctgct tgtccatatc ggagctggca ctgatcacct tcacggagat ggtcttctgt
      661 ccgacacctt ggcgccagag cgtgaccgcc acccggtcgc ccggcttctt gaagccgatg
      721 atctgctgca gctgcgccgt gtagttgacc ggctggccgt cgagcgcggt gatcacgtca
      781 ccctgctcga tgccggcgtc cttggccggg gattcaccgc ccggcgtgta gtcgtccacc
      841 acgacgccgt ggatgttgtc gtccggcatg ccgaccgcct tggcgtccgc ttcgcccgca
      901 tccgtcacgg tgacgccgat gaccgaccgg gtcacgtgcc cggaggccac gagctggtcg
      961 aagacggtgt ggacgaggtt gatcggaatg gcgaagccat agcccgtgta gtagccggtg
     1021 ccggaggcga tggcggaatt gatgccgatc acctgaccct tgatgttcac gagggggcca
     1081 cccgaattcc ccggattgat cgccgcgtcg gtctgaatga agtcctgaat ctggagcccg
     1141 ccgcgggcca ggccgagcaa cggacggccc ttggcgctca cgatgccggc ggtaacggtg
     1201 aagtcgaact cctcacccag cgggttgccg atcgccaata cccactcccc gatgttcgtc
     1261 gaatcggagt tgccgagcgt gaccgtcggc aggttgaggg cgtcgatctt gagcacggcg
     1321 acgtcggtct ggggatcggt ccccacgacg cgcacgttct tgaactcgcg cttgtcgaag
     1381 agcttcacgt tgacccggtc ggcgccggcg acgacgtggt tgttggtgag gatgtagccg
     1441 tccgcgctga cgatgaagcc ggtacccgag ccctcttcga cctgggggtg ccgcggctgt
     1501 cccgggatct ggaagaaatc ccggaacggg gcggggacct gcgaattgtc ctgccgctcg
     1561 gtgcgcgccg acgacacgta caccaccgcg ggacggacgc ggttcgagat ggcgacgaag
     1621 gcgtcgccga tctgcgcggc cgatttggcc tgcgggacct gagtctgcgc ggcggtcgag
     1681 agttgcggca acacggccgc ggtgctcttg ggcgtcaggt tgagagccga cgcgaacagc
     1741 aatccgaaca cgaatgctac gccgaccaga gaaacgaact tcagccaatc tcgtgtgcgt
     1801 gccgacatcg ccatctcgct ttgcgttaag ggttcgtcac ttcttgttct tgtcgtcgtc
     1861 tacgatttcg tagtccgcgt cgacgacgtt ctcctgcttg gcctcggtcc ccgcgttcgg
     1921 ctgggcttcc ggccccgcgc ccggtgccga gctggggtcg gctccacgcg actgcgcgta
     1981 gagtgacgca cccgcggccg aataggcctg ctgcagctcc tcgagcgcct tgccgatccc
     2041 ggcggcgtct ccggcgcgca aagcctgctt cgcgccgtcc accgcctggt ccagccgggt
     2101 cttcaagccc tgctcgagct tgtcggccca ttccttcgaa ttcttctcga cttcgtacac
     2161 catcgcgtcg agccggttgc ggcgctcgat ctcctcacgg cgatccttgt cttccgccgc
     2221 gtgggcctcc gcctccttga ccatcttgtc gatgtctttg tcaccgagcc cggacgacgc
     2281 ttcgatccgg atcttctgct ccttgccggt cgctttgtcc ttcgccgaga cgtgcaagat
     2341 gccgttcgcg tcgatgtcga acgtgacttc gatctgcggc accccgcgcg gagcgggcgg
     2401 aatccccgtc agctggaact tgccgatggt gcggttgtcg cgcgccatct gccgttcgcc
     2461 ctgcaatacg tggatctcca ccgtggtctg cgtatcctcg gccgttgaga acacctccga
     2521 cttcttggtc gggatggtgg tgttccgggg aatcagcacc gtggtcacgc cgcccagcgt
     2581 ttcgatcccg agcgagaggg gcgtgacgtc gagcagcagg acgtccttga cctcgcccgc
     2641 cagcacgcca ccctgaatcg cggcgccgat cgccaccact tcgtccgggt tgacgccctt
     2701 gtggggctcc ttcccgaaga acttctggac gacttcctgg accttcggca tccgggtctg
     2761 accgccgacc aggatgacct cgtcgatctc cgacggcttc agccccgcat ccttcagggc
     2821 ctgttccatc ggcgggatgg tgcgctggac cagggtgtcc accagctgct ccagcttggc
     2881 ccgggtcatc gacaggttca ggtgcttcgg tcccgactgg tccgccgtga tgaaggggag
     2941 gttgatctcc gtctgcatcg cggacgacag ctccatcttg gccttctc
//
LOCUS       DASUBP010000068        10503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_751115, whole genome shotgun sequence.
ACCESSION   DASUBP010000068 DASUBP010000000
VERSION     DASUBP010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 10503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10503
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751115"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(243..1226)
                     /locus_tag="VFU75_02760"
     CDS             complement(243..1226)
                     /locus_tag="VFU75_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HEU5465780.1"
                     /translation="MTALVAVDGLVKHYPTRRGWLGLKSSAPVRAVDGVSFSIPAGKT
                     LALVGETGSGKTTVGRTLLRLVEPTEGRFTFDGIDVFGLKSGPLRALRRRMQVVFQDP
                     SASLNPRMTVGDAIAEPLIIHGLAKRAERPARVAGLLGEVGLDPSFAPRYPHELSGGQ
                     RQRVGIARALAVRPDFLICDEPVTALDVSVQAQVLNLLRELQRAHGLTYLFIAHDLAV
                     VRHVADDVAVMYAGQIVERGPVDQIYSAPRHPYTRALLSAVPVPDPKAPHNRIVLSGD
                     VPSPTNPPSGCRFHPRCPHPLKDMRCSTDVPGLRELEDAQWAACHFADKPM"
     gene            complement(1223..2230)
                     /locus_tag="VFU75_02765"
     CDS             complement(1223..2230)
                     /locus_tag="VFU75_02765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5465781.1"
                     /translation="MSDANGPLLSIRDLKTYFVTGAGSARAVDGVSLELYPGETLGIV
                     GESGSGKTVTSLSVLRLVPEPPGHIMPGSLIEFEGRNLLTLSPGELRAVRGNQIAMVF
                     QEPMSSLNPVFTVGEQIAEVARVHLKVSKPEAWIRAVDMLNQVGVPDADRRAYEYPHQ
                     LSGGTCQRVMIAMALMCHPKVLIADEPTTALDVTVQAQILELLEKKQAEMGMAVMLIT
                     HDLGIVAGAADRVVVMYAGQVVETAGTADLFAGPLHPYTAGLLASVPRLDGGRGELTG
                     IPGTVPPATSWPAGCRFHPRCPHAWDRCRTQEPPLLEGPRPGQSARCWLVEEPNRRKA
                     P"
     gene            complement(2227..3153)
                     /locus_tag="VFU75_02770"
     CDS             complement(2227..3153)
                     /locus_tag="VFU75_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5465782.1"
                     /translation="MTARPTVALVGALVLAMGAVELLRLRARRTGSGRRFFAHRSAVW
                     GVRILLFFTIAALLAPLVAPYDPAAQPGIVTLQNMPPSWAHPFGTDAYSSDIWSRLVY
                     GGRVSLGIGLLATLVAMSVGVTIGAVAGYALGWIDAILMRLVDVGLAVPRIFMLLLAV
                     AYWNRIPLSILVLLLGLTGWFTTSRLVRAEALSLRGRPFVDAARALGASPTRVLIRHL
                     LPSALAPIIVSAVLSVGNVMLLEAGLSFLGFGVQPPTPSWGTMVADGQAQLALAPWTS
                     FFPGLTIALVVMSWNAVGDALRDAFDPRPEHA"
     gene            complement(3150..4133)
                     /locus_tag="VFU75_02775"
     CDS             complement(3150..4133)
                     /locus_tag="VFU75_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5465783.1"
                     /translation="MGGGGTWLIRRIAAAAAIVFAVVSLTFVSIHLAPGSPFLPPPDR
                     PLDTAVVARLNRQFGLDRPIRVQYVLYLKNLSQGNLGYAFSRGQTVRSALATAIPDTL
                     LLAGTALALDLGLGLILGIILAARARHATDLILSNVTLFIYSVPAFWLGLVLVFVFAD
                     WLHWFPAGFPTSPGLYESLPFFGQLGDRIHHLALPALTLGLVGAAGTARYQRAALLET
                     LGREFVRTARAKGVSERRVILVHALRNSLLPLITLAGLSLPFLLTGSVLIESVFSWPG
                     MGRMATDAVLARDHALVTAIAMVASAMVVTGSLVADVLYAAADPRIRERAS"
     gene            complement(4144..5781)
                     /locus_tag="VFU75_02780"
     CDS             complement(4144..5781)
                     /locus_tag="VFU75_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5465784.1"
                     /translation="MNFRVSLLAIGALPLALGCSSSGSCKGDYCGTVVFVTANNPKTL
                     LPPVIDNTGARDIDEQLFLRLADFGMTINTVGDSGWIPRLATRWEWDDAKTLVFHLDP
                     RARWHDGVPVTANDVAFSFDAYRDPVVGSSARNSLTKIASVTVRDSLTAVFRFKEQYP
                     TAFYDAVYQMWILPQHLLAQVPRTDWLTAAYGQHPVGDGPYRFTSMTPGQTYVLTADS
                     TFFLGRPHIRKLIWKIVSGPQAAVSDIVTGQADALDFLGSPANIDQAKKTPGVSVVPY
                     PGSNYMYLEFNTRANGDSSRPHPVFGDREVRRALTMALDRQAMSTNIFGPYSKVPPGP
                     IPQWWPIWDSVRTIGFDSAGAARMLAARGWRLGADGIRMKDSVRLSFEVIFTSTSAAR
                     MAFARLMQAQWKSVGVDMQIQPLDQNLIRDRLVSGKFDAVLQGFNSDPTPASGIAQHW
                     VRGAPDDFSHWTNDDFDRMVTEATSGTVSVKQSRGYWRAALNIINADAPGIWLAAPDN
                     VAAVSSRIADLTIRPDSYWALAWTWRIPADRLIDRDR"
     gene            complement(5808..6542)
                     /locus_tag="VFU75_02785"
     CDS             complement(5808..6542)
                     /locus_tag="VFU75_02785"
                     /inference="COORDINATES: protein motif:HMM:NF015084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465785.1"
                     /translation="MDWSVIVERAGRRGAMNMAVDQALLEEAGHTGRAYLRLYRWTPS
                     CLSFGRNEPALTRYDRAEIERRGISVVRRPTGGRAVWHDRELTYAVAAPIAAFGTLAQ
                     AYRIIHERIATALGTVGVVATVAPAARPESIGAGACFASAVGGEVVAGRGKIVGSAQV
                     ASGSAFLQHGSILLDGSQELVRSVTRGPAPAGGETTLSNLAKRAVSFDDVADAIVSSW
                     TPPPIPAPAPSVPPGRFAEADWTWRR"
     gene            complement(6600..9488)
                     /gene="gcvP"
                     /locus_tag="VFU75_02790"
     CDS             complement(6600..9488)
                     /gene="gcvP"
                     /locus_tag="VFU75_02790"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HEU5465786.1"
                     /translation="MAIAPASLVHPDRFASRHLGPDPDEVREMLKVVGYASLDAFIDA
                     VIPDDIRFRKPLNLPSARSERETLAALRALASKNQVFRSYIGMGYSDCITPPVVLRNV
                     LENPGWYTAYTPYQAEIAQGRLEALLNYQTMVSDLTGLEVANASLLDEGTAAAEAVHL
                     TAAATSHDGPGAFLIDSNCHPQTIAVVRTRAEAVGIQVTVADPKSFSFKPGTIGCLLQ
                     YPGTEGDIQDFRALAEKAHAAGALVTAATDLLALTLLTPPGEWGADIAVGNSQRFGVP
                     LGYGGPHAAFFATKDAYKRHLPGRIIGVSKDRAGRPGLRMALQTREQHIRREKATSNV
                     CTAQVLLAVMASMYAVYHGPDGIKKIAERVHGLAAILAEGARRLGYKIGHAAFFDTIV
                     IEGDEAKIKEVIAGAKRRRINLRPLGATRVGVALDETVGLEDVEDLAQVLALGKPLPF
                     KLEDLIAAAKSAIPQGLARTTPYLTHPVFHRHRSETEMLRYLKQLEDRDLSLTSAMIP
                     LGSCTMKLNATTEMIPVTWSEFGRIHPFAPRDQAQGYTELFRQLEEWLAEITGFARVS
                     LQPNAGSQGEFAGLLVIRAYHRARGQAHRTTCLIPQSAHGTNPASAVLAGMNVVVVKT
                     DAQGNIDVADLAAKAEQHKDTLAALMVTYPSTHGVFESSIREICKIVHAQGGQVYMDG
                     ANMNAQVGLCRPGDFGADVCHLNLHKTFCIPHGGGGPGMGPIGVAAHLASHLPDHPVV
                     PLGATESCGTVSAGPWGSPSILPISWAYIAMMGKEELPEATKLAILSANYIAKRLAGH
                     YGLLYAGKNGLIAHECILDTRPFKTSAGVEVEDIAKRIIDYGFHPPTVSFPVAGTLMV
                     EPTESEPKVELDRFCDALIAIRNEIREIERGEADREDNLLTNAPHPLADLLADEWKHP
                     YSRERAAFPSPATRAHKVWPTVSRIDGAYGDRNLICTCPPVESYATVG"
     gene            complement(9526..9915)
                     /gene="gcvH"
                     /locus_tag="VFU75_02795"
     CDS             complement(9526..9915)
                     /gene="gcvH"
                     /locus_tag="VFU75_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HEU5465787.1"
                     /translation="MANVPADLSYTAEHEYVKTTSDASVVQVGITDYAQGELGDVVYL
                     DLPKAGASFAKGAVFGTIEAVKAVSDLYCPVAGTVVESNPALDKDPGAVNREPYGAGW
                     MIKLKIKDQGDLKGLMNAAAYEKHIGK"
     gene            complement(9962..>10503)
                     /gene="coaE"
                     /locus_tag="VFU75_02800"
     CDS             complement(9962..>10503)
                     /gene="coaE"
                     /locus_tag="VFU75_02800"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEU5465788.1"
                     /translation="RWGAVIIDADQLAREAQAPGTEVLAAIVRRFGGDVLTSDGALDR
                     AALRSKVMGDDQALGALNGIVHPAVQRRRAQLLDEARARGDRVVINDIPLLFEALDPK
                     DFDAVVLVDAPPAVRRARLRLLRGLTNAEADAMIAAQMPSDRKRGKSQYVIDNDGSKE
                     ALEQKARAVFDELTKRANT"
BASE COUNT         1769 a         3394 c         3712 g         1628 t
ORIGIN      
        1 ggcgacggac gaggagatgc tcgcggccgt gcggcagggc ttcaagggtg aggtcgtgat
       61 ggggagggat ctgggggagt actgacccgg gacggcgggc atggtctctc gatagccccg
      121 gacttgcgtc cggggttatg aaaaccgcaa cagccatttg cagtgatctc aagctcagtc
      181 gaggtggggg tagcgcagtt cataaccccg ggcgcaagcc cggggaattt gaggggctgg
      241 gtctacatcg gtttgtccgc aaaatgacaa gctgcccact gtgcatcctc gagctcgcgt
      301 agcccgggaa cgtccgtaga gcacctcatg tccttcagcg gatgcgggca gcgggggtgg
      361 aaccggcacc ctgacggcgg gttcgtcggg gacggcacgt cgccggacag gacgatccgg
      421 ttgtgcggag ccttggggtc gggcaccggg accgcggaga gcagggcgcg ggtgtagggg
      481 tgccggggag cggagtagat ctggtcgacc ggtccccgtt cgacgatctg tccggcgtac
      541 atcacggcca cgtcgtcggc gacgtgacgg accaccgcca gatcgtgcgc gatgaagaga
      601 taggtgaggc cgtgggcccg ctgaagctcg cggagcaggt tgagcacctg cgcctgcacg
      661 gacacgtcga gcgcggtgac gggctcgtcg cagatcagga agtcggggcg gaccgcgagg
      721 gcgcgggcga tccccacccg ttgccgctgg ccacccgaca gctcgtgcgg ataccgcggc
      781 gcgaatgacg ggtcgagtcc cacctcgccg agcagtccgg ccactcgagc ggggcgctcc
      841 gcccgcttcg cgagaccgtg gatgatcagg ggctccgcga tcgcgtcacc caccgtcatc
      901 cggggattca gcgaggccga cggatcctgg aacaccacct gcatcctccg ccgcagggcc
      961 cgcaggggac cgctcttcag cccgaacacg tcgatcccgt cgaacgtgaa gcgcccctcg
     1021 gtgggctcca ccaggcgcag gagggtacgg ccgaccgtgg tctttccgga gcccgtctcc
     1081 ccgaccagcg cgagcgtctt cccggcggga atcgagaacg agacgccatc caccgcgcgc
     1141 accggcgccg acgacttgag cccgagccac ccccgtcggg tggggtagtg tttgaccagt
     1201 ccgtcgactg cgacgagcgc ggtcacgggg ccttccggcg gttgggctct tccaccagcc
     1261 agcagcgggc ggattggccc ggccgcggtc cttcgagcag cggcggctcc tgggtgcggc
     1321 aacggtccca ggcatgcggg cagcgggggt ggaaccggca ccccgcgggc cagctggtgg
     1381 cgggcggcac cgtgccgggg atgccggtga gttctccccg tccaccatcg aggcgcggca
     1441 ccgaggcgag cagtcccgcc gtgtaggggt gcagggggcc ggcgaacaag tcggccgtgc
     1501 cggccgtctc cacgacctgt ccggcgtaca tcacgaccac ccgatcggcg gccccggcca
     1561 cgatgcccag atcgtgcgtg atcagcatga cggccatccc catctccgcc tgcttcttct
     1621 cgagcagctc gaggatttgc gcctgcacgg tcacgtcgag cgcggtggtc ggctcatcgg
     1681 cgatgaggac cttggggtgg cacatgaggg ccatcgcgat catcacgcgt tggcaggtgc
     1741 cgcccgagag ctggtgggga tactcgtacg cccggcggtc cgcgtcgggc acgccgacct
     1801 ggttcagcat gtcgacggcg cgaatccatg cctcgggctt cgagaccttg agatgcacgc
     1861 gggcgacttc ggcgatctgc tcgcccacgg tgaacacggg gttgagggag ctcatcggct
     1921 cctgaaacac catcgcgatc tgattcccgc gcaccgcccg cagctcgccg ggcgagaggg
     1981 tgaggagatt gcggccttcg aactcgatga ggctcccggg catgatgtga ccgggtggct
     2041 caggaacgag gcgcaggacc gagaggctgg tcaccgtctt ccccgacccc gactcgccca
     2101 cgatgccgag cgtttcgccc gggtagagct cgagggacac gccatccacg gcacgggcgg
     2161 agccggcacc cgtgacgaag taggtcttca gatcccggat ggacagcagg gggccgttgg
     2221 cgtcgctcaa gcgtgctccg ggcgtggatc gaacgcgtcg cgcagggcgt cgcccacggc
     2281 attccaggac atgacgacga gggcgatggt caggcccgga aagaagctcg tccagggcgc
     2341 gagcgccagt tgggcctgcc cgtccgccac catcgtcccc cacgacggcg tggggggctg
     2401 tacgccgaag ccgaggaacg aaaggcccgc ctcgaggagc atgacgttgc cgaccgaaag
     2461 cacggcggac acgatgatcg gggcgagggc ggacgggagc agatgacgga tcaggacccg
     2521 cgtcggggag gcgccgaggg cgcgcgcggc gtcgacgaac ggacggcccc gaagggaaag
     2581 cgcttccgct cgcaccaggc ggctggtcgt gaaccatccg gtcaacccga ggagcaggac
     2641 gagaatggac agggggatcc ggttccagta cgcgaccgcg agcaacagca tgaagatccg
     2701 cgggacggcg aggcccacgt ccaccaaccg catcaggatg gcgtcgatcc accccagcgc
     2761 atatccggcc accgcgccga tcgtgacacc gacggacatg gcgaccagcg tcgccaggag
     2821 cccgataccg agggacacgc gtcctccgta tacgaggcgg ctccagatat ccgagctgta
     2881 ggcgtcggtg ccgaaggggt gggcccacga cggcggcatg ttctgcaaag tgacgatgcc
     2941 gggttgcgcc gcggggtcgt acggtgcgac caggggcgcc aggagagcgg cgatcgtgaa
     3001 gaagaggagg atgcgtactc cccagacggc cgaccggtgc gcgaagaagc gccggccgct
     3061 cccggtgcgt cgcgctcgca gccggaggag ctcgacggca cccatcgcga gcacgagtgc
     3121 gcccacgagg gcgaccgtgg gtcgggccgt cacgaggcgc gctcgcgaat tctcgggtcc
     3181 gcggcggcgt agaggacgtc ggccacgagt gagccggtca ccaccatcgc gctggcgacc
     3241 atggcgatcg cggtgaccaa cgcgtggtca cgggcgagca cggcatcggt ggccatgcgt
     3301 cccatcccgg gccaggagaa gacgctttcg atcagcaccg agccggtgag caggaagggc
     3361 agggacagac cggcgagcgt gatcagcggc agcagggaat tccgaagcgc gtggacgagg
     3421 atgacgcgcc gctcgctcac gcccttcgcc cgtgcggtcc gcacgaactc gcgtcccagc
     3481 gtctcgagga gcgcggcgcg ctgataccgc gccgtgccgg ccgcgccgac caggccgagc
     3541 gtgagggcgg gaagggccaa gtgatggatg cggtccccca gctggccgaa gaacggcaga
     3601 ctctcgtaca acccgggcga ggtcgggaat cccgctggaa accagtgcag ccagtcggcg
     3661 aacacgaaca ccagaacgag acccaaccag aacgcgggca cggaatagat gaacagtgtg
     3721 acgttggaga gaatgaggtc ggtcgcgtgc cgggcgcggg ccgccaggat gatcccaagg
     3781 atgagtccca gacccagatc caatgcgagt gcggtgccgg cgaggaggag ggtgtccggg
     3841 atggcggtcg ccagggcact ccgaaccgtc tgaccacggc tgaaggcgta gccgaggttc
     3901 ccctgcgaca ggttcttgag atacagcacg tattggacgc gaatcgggcg gtcgagcccg
     3961 aattgccggt tgaggcgcgc gacgacggcg gtgtcgagtg gccggtccgg cgggggcagg
     4021 aacggagacc ccggcgccag gtggatcgag acaaaggtga gggagacgac ggcgaagacg
     4081 atggcggccg ccgcggcgat gcgccggatc agccaggtcc cgccgccacc cacgtcaggg
     4141 tccctagcga tctcgatcga tgaggcggtc ggcggggatc cgccaagtcc acgcgagggc
     4201 ccagtaggaa tcgggcctga tcgtgaggtc cgcgatgcgc gagctgacgg cggcgacgtt
     4261 gtccggcgcg gcgagccaga tcccgggggc atcggcattg atgatgttca gcgccgcccg
     4321 ccagtagccg cgtgattgct tcacggacac cgtgccggac gtggcctccg tcaccatgcg
     4381 atcaaagtcg tcgttggtcc aatggctgaa gtcgtcgggc gcgccccgga cccagtgctg
     4441 ggcgatgccg gacgcggggg tcggatccga gttgaagccc tgcagcaccg cgtcgaactt
     4501 gcccgacacc agccggtcgc ggatgagatt ctgatccagc ggctggatct gcatgtctac
     4561 accgacggac ttccactgcg cctgcatcag ccgcgcgaaa gccatgcgcg cggcgctggt
     4621 cgaggtgaag atcacctcga acgacagccg cacgctgtcc ttcatccgga tgccatcggc
     4681 tcccagccgc catccgcgcg ccgccagcat tcgggcggca cccgcgctgt cgaacccgat
     4741 ggtccgtacc gagtcccaga tgggccacca ctgcgggatc gggcccggcg gaaccttcga
     4801 gtacgggccg aagatgttcg tcgacatggc ctggcgatcg agcgccatcg tgagcgcccg
     4861 ccggacctcc cggtcgccga agaccgggtg tggcctggag ctgtccccgt tggcccgggt
     4921 gttgaactcc aggtacatgt agttcgaccc cgggtagggg actacggaca cgcccggggt
     4981 cttcttcgcc tggtcgatgt tggcgggcga gcccaggaaa tcgagggcat ccgcctggcc
     5041 ggtcacgatg tcggagacgg cggcctgtgg cccgctcacg attttccaga tcagcttccg
     5101 gatgtgcggg cggcccagga agaacgtcga gtccgccgtc agcacgtacg tctgccccgg
     5161 cgtcatgctg gtgaaccggt agggcccgtc gccgacgggg tgttgcccgt aggccgccgt
     5221 gagccagtcg gtccgcggga cttgcgcgag cagatgctga gggaggatcc acatctggta
     5281 gacggcgtcg tagaatgccg tcgggtactg ctccttgaac cggaaaaccg cggtcaggga
     5341 gtctcgcacg gtcacggacg cgattttggt cagggagttg cgcgcggaag agccgaccac
     5401 cgggtcgcgg tacgcatcga acgagaacgc gacgtcgttg gccgtcaccg gaacgccgtc
     5461 gtgccagcgg gcccgcggat cgagatggaa gaccagcgtc ttcgcgtcgt cccattccca
     5521 gcgggtggcg agccgtggga tccatccgga atcaccgacc gtgttgatgg tcatgccgaa
     5581 atcggcgagc cggaggaaca actgctcgtc gatgtcccgc gcgccggtgt tgtcgatcac
     5641 cggggggagc aaggtcttgg ggttgttcgc ggtgacgaac acgaccgtac cgcagtagtc
     5701 gcccttgcat gagcccgagg acgaacagcc gagggcgagt ggcagggcac cgatcgccag
     5761 cagagacacg cggaagttca tacgccgcct aacattcaat gtcccgtcta tcggcgccag
     5821 gtccaatcgg cttccgcaaa gcgccccggt ggcaccgaag gggcgggcgc cgggatcggt
     5881 gggggggtcc aggacgaaac gatggcgtcg gccacgtcat cgaatgacac tgcgcgcttc
     5941 gcgaggttcg acagcgtcgt ctcaccaccg gctggggccg gcccgcgggt caccgaccgc
     6001 accagttcct gagacccgtc gagcaggatc gagccgtgct gcagaaacgc gctgccgctc
     6061 gcgacctgag cggaccccac gattttcccc ctgccggcca cgacctcacc gcccacggcc
     6121 gacgcaaagc aggcgcccgc accgatcgac tcgggacgag cagcgggtgc gacggtcgca
     6181 accacgccca ccgtacccag cgccgtcgcg atccgttcat gaatgatccg gtacgcctgg
     6241 gccagggtgc cgaacgccgc gatgggcgcg gcgacggcgt aggtcagctc gcggtcgtgc
     6301 cacaccgcgc gcccacccgt gggccggcgc accacagaaa tgccacggcg ctcgatttcc
     6361 gcgcgatcat atcgggtgag ggccggttcg ttacgtccaa aggaaagaca ggaaggggtc
     6421 cagcgataca gccggagata cgcccggccc gtatggccgg cttcttcaag gagggcctgg
     6481 tcgacggcca tattcatggc tccacgacgc ccggcgcgct caacgatgac ggaccagtcc
     6541 atgatctaga cggctagacg ggtggacggc tagacgaggg caactagccg tctagccgtc
     6601 taaccgactg tggcgtacga ctccaccggc ggacacgtgc agatcaagtt ccggtctcca
     6661 tacgcgccgt cgatccggct gacggtgggc cacaccttgt gtgcgcgcgt ggccggcgat
     6721 gggaaggcgg cccgctcccg cgaataggga tgcttccact cgtccgcgag caagtccgcc
     6781 agcgggtgag gcgcattggt caggagattg tcctcgcggt ccgcctcgcc gcgctcgatt
     6841 tcccggatct cgttccggat cgcgatcaag gcgtcgcaga accggtccaa ctccaccttc
     6901 ggctcacttt ccgtgggctc caccatgagc gtgcccgcga cggggaagga gaccgtgggc
     6961 gggtggaagc cgtagtcgat gatgcgcttg gcgatgtcct cgacctcgac tcccgccgac
     7021 gtcttgaagg gccgcgtgtc gagaatgcac tcgtgcgcga tgagcccgtt cttcccggcg
     7081 tagaggagcc cgtaatgacc cgccaggcgt ttggcgatgt aattcgcgga caggatcgcg
     7141 agcttggtcg cctcggggag ctcttccttc cccatcatcg cgatgtacgc ccacgaaatc
     7201 ggcaggatgc tgggggagcc ccagggcccc gcggagaccg tcccgcagct ttccgtcgcc
     7261 ccgaggggca ccaccgggtg atcggggaga tgactggcga ggtgcgcggc caccccgatc
     7321 ggtcccatgc cggggccgcc gccgccgtga ggaatgcaga acgtcttgtg aaggttcagg
     7381 tggcacacat cggcgccgaa gtccccgggc cggcagaggc ccacctgggc attcatgttg
     7441 gcgccatcca tgtagacctg gccgccctgc gcgtggacga tcttgcagat ctcgcggatc
     7501 gaagactcga acacgccatg cgtcgacgga tacgtcacca tcaacgccgc gagcgtgtcc
     7561 ttgtgctgct cggccttcgc ggcgagatcg gcgacgtcga tgttgccctg cgcgtccgtc
     7621 ttcaccacca ccacgttcat gccggccagt acggcgctcg cggggttggt cccatgcgcc
     7681 gactggggaa tgagacatgt ggtgcgatgc gcctggccgc gggcgcgatg gtacgcacgg
     7741 atcacgagga gaccggcgaa ctcgccctgc gacccggcgt tgggttgcag ggagacgcgc
     7801 gcgaacccgg tgatttccgc gagccattcc tcgagctgcc ggaagagctc ggtgtacccc
     7861 tgcgcctgat cgcgcggggc gaacggatgg atcctcccga actcagacca ggtcaccgga
     7921 atcatctcgg tcgtggcgtt cagcttcatc gtgcacgagc cgagcgggat catggccgag
     7981 gtgagcgaca ggtcccggtc ttcgagttgt ttgagatagc gcagcatctc cgtttccgac
     8041 cggtgccggt ggaatacggg gtgcgtgaga tacggggtgg tgcgggccag tccctgcggg
     8101 atggcactct tcgcggccgc gatcaggtct tcgagcttga agggcagcgg cttgccgagg
     8161 gcgagcacct gggccaggtc ctcgacatct tcgaggccca cggtctcgtc gagcgcgacg
     8221 ccgacgcggg tggcgccgag ggggcggagg ttgatccgcc ggcgcttggc accggcgatc
     8281 acttccttga tcttcgcctc gtcgccttcg atcacgatcg tgtcgaagaa tgcggcgtgg
     8341 ccgatcttgt agcccagacg gcgagcccct tcggcgagaa tcgcagccag gccgtgcacg
     8401 cgctccgcga tcttcttgat gccgtccggg ccgtgataga cggcgtacat gctcgccatg
     8461 acggccagga ggacctgcgc ggtgcagacg ttgctcgtcg ccttctcgcg gcggatgtgc
     8521 tgctcgcgcg tctgcagggc catccggagt ccgggacggc cggcgcgatc cttcgagacg
     8581 ccgatgatgc gtccgggcag atgccgcttg tacgcgtcct tcgtcgcgaa gaacgccgcg
     8641 tgcggcccgc cgtacccaag cggcacaccg aagcgctggg agttgccgac ggcgatgtcc
     8701 gcaccccact cacccggagg cgtcagcagc gtgagcgcca acagatcggt cgccgcggtg
     8761 accaacgcgc cggcggcgtg ggccttctcg gccagcgcgc ggaagtcctg gatgtccccc
     8821 tcggttcccg gatactgcag caggcagccg atggtcccgg gcttgaagga aaagctcttg
     8881 gggtccgcca ccgtcacctg aatgcccact gcttcggccc gggtccggac cacggcgatg
     8941 gtctgcgggt ggcagttgga gtcgatcaga aaggcgccgg ggccgtcatg gctggtcgcg
     9001 gcggcggtca gatgcaccgc ctcggcggcc gccgtgccct cgtccaggag cgaggcattc
     9061 gccacctcga gtccggtgag gtccgagacc atcgtctgat aattgagaag cgcctcgagc
     9121 cgtccctggg cgatctcggc ctgatatggc gtatacgccg tgtaccagcc cggattctcc
     9181 agcacgttgc gcagcacgac ggggggcgtg atgcagtcgg agtaccccat cccgatgtat
     9241 gaccggaaga cctgattctt cgacgcgagc gcgcgcaggg ccgcgagggt ctcgcgctcg
     9301 ctccgcgcgc tggggaggtt caggggtttc cggaaccgga tgtcatcggg gatgacggcg
     9361 tcgatgaacg cgtccagaga ggcgtagccg accaccttca gcatctcgcg gacctcgtcg
     9421 gggtcgggcc ccaggtgccg ggatgcaaag cggtcgggat gaacgaggga tgcgggggcg
     9481 atcgccatgg attttctgcc gcctgacgtc tgaagtctcg aggtcttact tcccgatgtg
     9541 cttctcgtat gcagcggcat tcatcagacc tttcaggtca ccctggtcct tgattttcaa
     9601 cttgatcatc cagcccgcgc cgtatggctc acgattcacg gcccccgggt ccttatcgag
     9661 ggcggggttg ctttccacca ccgtgccggc caccgggcag tacaggtcgg aaacggcttt
     9721 gaccgcctcg atggttccaa acaccgcccc cttggcgaac gaggcgcctg ccttcggcag
     9781 gtcgaggtag accacatccc ccagctcacc ctgggcgtag tcggtgatgc cgacttggac
     9841 gaccgacgca tcactcgtcg ttttgacgta ctcgtgttcc gcggtatagg agaggtcggc
     9901 gggaacgttg gccatggcgc gggtccgtgt tggaaggtgt gggaaatcgg ggcgaaggtt
     9961 atcaggtatt tgcgcgtttc gtcaactcat cgaacacagc cctggccttc tgctctaacg
    10021 cctctttcga cccgtcattg tcgatgacgt attgggattt cccgcgcttc cggtccgacg
    10081 gcatctgggc cgcgatcatg gcgtcggcct cggcattggt caggccccgc aggaggcgga
    10141 gccgggcgcg acggacggcg gggggtgcgt ccaccaggac caccgcgtcg aagtccttcg
    10201 ggtccaaggc ctcgaagagc agggggatgt cgttgatcac cacccggtcc ccccgggccc
    10261 gggcttcgtc caggagctgt gcccgacgtc gctgtacggc gggatggacg atgccgttga
    10321 gtgcgccgag cgcctgatcg tctcccatca ccttggaacg cagagcggcc cggtccagag
    10381 cgccgtcgga cgtgagcacg tccccaccaa accggcggac gatggccgcg aggacctcgg
    10441 tgccgggagc ctgggcttcc cgggcgagct ggtcggcgtc gatgatcacg gctccccatc
    10501 tcc
//
LOCUS       DASUBP010000069         7072 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_696459, whole genome shotgun sequence.
ACCESSION   DASUBP010000069 DASUBP010000000
VERSION     DASUBP010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7072)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7072)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7072
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696459"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1587
                     /locus_tag="VFU75_02805"
     CDS             <1..1587
                     /locus_tag="VFU75_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEU5465789.1"
                     /translation="LLGERASGDVVRPGAEKTVVEGAFDLAGTPGIAAVRAVADELGV
                     ELSEETLVIRREVYPEGRSRAWVNGSPTTVGVLAQLGSALVDLHGQHETQSLVKGEAQ
                     AGILDAYADAQVERTAVRDAYARWQGAVTRENELKGKQEEVRRKADYLRHVVEEITQA
                     APKVGEDEVLETEAKRLANAEELGRLARELEEAADQSGLSKGARVLAALERIDPAVGK
                     WREMLDTAFTNLQELSAAARDYAASIDADPARLATIDRRRDILDKLTRKYGPEIPDVL
                     ATRDASARELDLLDTAELDLRSLAEAREQASRDFVRACEALSKKRTAGARKLGKAVNE
                     ILPSLGLGGGTFDPGLRPLPTPSSQGAEQVSFEVELSPGMGSKPLSRVASGGETSRLM
                     LALKVVLAGHDAIPTLVFDEVDQGIGGEVGGKVGEALAEVADGRQVLVITHLPQIAVR
                     ADHHVVITKGARGGVATSDIKETSGEDRTREVARMLGDPDLEAALKHARELLRTARET
                     SGRRSGTSSPPEPKSPRQSR"
     gene            complement(1505..3043)
                     /locus_tag="VFU75_02810"
     CDS             complement(1505..3043)
                     /locus_tag="VFU75_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465790.1"
                     /translation="MTKTFAVLALVLVLPGALPAQAAERADTPRKGKVRLSFMPVIET
                     WEAAFGPQGRQPVGGFLTSDSLGAAQVPSLSGMQSAIQTASGINAFSASLGRTLLDIR
                     SERRVIPLSAEVGLTDRLSIGVMVPLVRVDVRETLNVDSAHGNLGLNPLLLGSTAFTT
                     FFANFNNALTALNNEIQGGTCVPRCTEAQALYNQGVALRNALGQAVYGTGPSSQSPFL
                     PLASSAAGKAIDTTVITLQRQLVDSFGISGFADTLGLPTLAAASTDIEQVLSDPAFGY
                     EYAPIQRTPKFYRYWPGDAEVSAKYRMIVTSTYAGALKVTVRLPTGHLASPNDPFAIS
                     SGDHQTDVELGYIQELTLWNRLWLNLNVRGGVQMQGFRDARVAPRGAFWVPIGASARL
                     NWKPGNYLGIDVAPMYRFSKTFAAGFTASYYRQGLDTYTYQSPADSLRVAGNLGAPVS
                     AAVLDPGTNRHFTQLGLAVTYQGPVWETGFSIQQTVSGFGAGTSVPAATTFRIVFRTF
                     HGLF"
     gene            complement(3113..4351)
                     /locus_tag="VFU75_02815"
     CDS             complement(3113..4351)
                     /locus_tag="VFU75_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465791.1"
                     /translation="MTKTTIRYLAALGILLGAAAACNNDELFHNSTEDPVSPLFTRYV
                     SMGNSITAGFQSNGINDSTQRQAYAVLVARQMHTPFYQPLLNKPGCAPPYTNVFTQAR
                     LGGGTSQTCLLREGQTPPPPYISDVAVPGATASSPTNNLVAGSNSNLLTQLFLGGLTQ
                     IGMMRKVQPTFVTVWIGNNDVLGAATDQTHPGDSLEVTDTATFKTEYKAMLDSIDAIG
                     SVKGGVLIGVADVTQIPYASLGAVYFQLKATKLAALPSFQVDASCAPSALGGVGDSSL
                     VPFFYGLGLIAAAQAAPTGGPFVLNCGTDDLVITKPEFLKLHNSVLAFNAYISAQATA
                     RGYAYFDPNVALAQLKADTTQVAFFPHAPPDPRATTAPFGTAFSFDGVHPSAATHKLV
                     ANTLIAVINAKYGTSLKTIP"
     gene            complement(4365..5735)
                     /locus_tag="VFU75_02820"
     CDS             complement(4365..5735)
                     /locus_tag="VFU75_02820"
                     /inference="COORDINATES: protein motif:HMM:NF015316.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HEU5465792.1"
                     /translation="MRGTALVLAVGLIPAALGAQGFGVYELSTCTMARGGAVAANPCG
                     DGSAVFFNPAGLSSLSGTHFSAGLTTISPTGGFTDDVFLQHTGMTSQTFVVPNFFLTH
                     RLNEKIGIGLGFYVPYGLGTKWPLSFAGRFAGYDTQVKSYYIQPTISYQVTPRLSFGL
                     GVAYVHASVALHQHLDLSQQLVPTAITGPAGLPAGLTFAQVGIPTNTDFANADLSASG
                     NGIAFNGGVIFKVNDQLTLGGHFLTRKTIHFTGNASFQEIKTGLVLYAPPLINSPLPL
                     DAVLPSEFASGGPLSGSAATTDVTLPDQGSIGFDYKFNDKWHLSGDLQTVVWGWFNQL
                     TVNFANAGTPPLSDYEGYRDSYGVRLGTEYAYSPKLTFRGGYVFNTAAAPDQTVTPRL
                     PEGSRNSFILGVGYQLTGALHGDASVMYLKQNDRRGRVSEVSTLNTGLYTFKAVLLGA
                     GLSYTF"
     gene            5822..6964
                     /gene="galK"
                     /locus_tag="VFU75_02825"
     CDS             5822..6964
                     /gene="galK"
                     /locus_tag="VFU75_02825"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEU5465793.1"
                     /translation="MKESFTLFRETYGVAPTIGGSAPGRVNLIGEHTDYNGGPVLPMA
                     IARRTVVMAGPGDPGVIEVISARDGELRRVEFLREPGEGTTGFVVGVARSLDRLGIAL
                     PNGGRVAITSDVPIGAGLASSAALTVSAACALAALAGQKLPAKEAARVGYQAEHDHVG
                     VRCGTMDQTIAALGRKGHALLLECATGATKQIPFRGTLLLVDTGVRHDLAHSAFNTRR
                     AECEAALEALRKVHPGLRWLALWPAEEIPRLARLLPSPLRARAQHVVGESVRTRAAAM
                     LLARGRLANFGKLMYQSHESCRRLYECSAPELDLVVQAAKRAGAMGARMTGAGWGGAA
                     LVLIRPKAAERTGSAIARAFERRFGRAPVITLLQGGAGARVEKVRG"
BASE COUNT         1191 a         2189 c         2562 g         1130 t
ORIGIN      
        1 ctcctggggg agcgcgcgtc cggggatgtc gtccgccccg gtgccgagaa gacggtggtc
       61 gaaggggcgt tcgatttggc cgggactccc ggcatcgcgg ccgtccgcgc ggtcgcggac
      121 gagctcggcg tcgagctctc cgaagagacc ctggtcatcc gccgggaggt ctatcccgag
      181 ggccgctccc gtgcctgggt gaacgggtct ccgaccacgg tcggcgtgct cgcgcagctg
      241 gggtcggcgc tcgtggacct ccatggacag cacgagaccc agtcactcgt gaagggggaa
      301 gcgcaggccg ggattctcga cgcctatgcc gacgcgcagg tcgagcggac ggcggtgcgc
      361 gacgcctatg cgcggtggca gggcgcggtg accagggaaa acgagctcaa gggcaagcag
      421 gaggaggttc gccggaaggc ggactacctg cgccatgtcg tcgaagagat cacccaggcc
      481 gcgcccaagg tcggggagga cgaggttctc gagaccgaag cgaagagact ggcgaacgcc
      541 gaagagctgg gccggctggc gcgtgagctg gaggaggcgg cggatcaatc cggactctcg
      601 aagggcgcga gggtcctggc cgcactcgag cggatcgatc cggcggtggg gaagtggcgc
      661 gagatgctcg acacggcgtt cacgaacctt caggagctgt ccgccgccgc gcgggactac
      721 gccgcctcga ttgacgcgga tccggcgcgc ctggccacca tcgaccgccg ccgcgacatc
      781 ctcgacaaac tcacccggaa atacggcccc gaaatccccg acgtcctggc cacccgcgac
      841 gcctccgcac gagagctgga cctgctcgac accgccgagc tggacctgcg gtctctcgcc
      901 gaggcccgcg agcaggcctc gcgggatttc gtccgggcct gcgaagcgct gtcgaagaag
      961 cggactgcgg gagccaggaa gctgggcaag gccgtgaacg agatcctgcc gtccctcggg
     1021 ttgggtggag ggacgtttga tcctggactg cgacccctcc ctactccctc ctcccaaggt
     1081 gccgagcagg tctcgttcga agtcgaactc tctcccggca tgggctcgaa gcccctctcc
     1141 cgcgtcgcgt cgggcgggga gacatcacgg ctgatgctgg ccctcaaggt ggtcctcgcg
     1201 ggtcacgacg cgatccccac cctcgtgttc gacgaggtgg accagggaat cggcggcgag
     1261 gtgggcggga aggtgggaga ggcgctggca gaggtggctg atgggcgtca ggtgctggtg
     1321 atcacgcacc ttccgcagat cgcggtgcgc gcggaccacc acgtcgtcat caccaagggc
     1381 gcgcggggcg gagtggccac cagcgatatc aaggagacct caggggagga tcgcacgcgc
     1441 gaagtggcgc gcatgctcgg cgatcccgac ctcgaagccg ccctcaagca tgcccgcgag
     1501 ctgcttagaa cagcccgtga aacgtccgga agacgatccg gaacgtcgtc gccgccggaa
     1561 ccgaagtccc cgcgccaaag ccgctgaccg tctgctgaat cgaaaatccg gtctcccaga
     1621 ccggtccctg gtacgtgacg gccaatccca gttgcgtgaa gtgccgattc gttccgggat
     1681 cgagcaccgc ggcgctcacc ggcgcaccga gattccccgc gacgcggagt gagtcggcgg
     1741 gggactggta ggtgtacgtg tcgagcccct ggcggtagta cgacgccgtg aaccctgccg
     1801 caaacgtctt cgagaagcgg tacatcggcg cgacatcgat gcccaggtag ttgccgggct
     1861 tccagttgag ccgggcactc gccccgatcg gcacccagaa ggcgccccgc ggcgcgaccc
     1921 gcgcatcccg gaagccctgc atttgcacgc caccccggac attcagattc agccagagcc
     1981 gattccagag cgtgagctcc tggatgtagc cgagctcgac gtcggtctgg tgatcgcccg
     2041 acgaaatcgc gaacggatcg ttgggggagg ccaggtgacc ggtgggcagg cgcaccgtga
     2101 ccttgagcgc gcccgcatac gtggacgtca cgatcatgcg gtatttcgcc gagacttcgg
     2161 catccccggg ccagtaccgg tagaacttgg gcgtccgctg gatgggcgcg tactcgtagc
     2221 cgaaggccgg atcgctcagg acctgctcga tatccgtgga cgccgcggca agggtcggaa
     2281 gcccgagcgt gtcggcgaat cccgaaatcc cgaatgagtc cacgagctgg cgttggaggg
     2341 tgatgacggt ggtgtcgatg gccttgccgg cggcggagct ggcgagcggg aggaagggag
     2401 actggcttga cggacccgta ccgtagactg cctggcccag cgcgttccgg agggccacgc
     2461 cttgattgta cagcgcctgc gcttcggtgc agcgggggac gcaggtcccg ccctggatct
     2521 cgttgttgag cgccgtgagg gcgttgttga agttcgcgaa aaaggtggtg aacgccgtgc
     2581 tccccagcag aaggggattg agtcccaggt tgccgtgtgc cgagtcgacg ttcagcgtct
     2641 cccggacgtc cacccggacc agcgggacca tcacgccgat cgagagccgg tcggtgagcc
     2701 cgacctcggc ggagagagga atcacccgcc gttccgaacg gatgtcgagc aaggtgcggc
     2761 cgagactggc ggagaacgca ttgataccgc tcgccgtctg aatcgccgac tgcattcccg
     2821 agagggacgg gacttgagcc gcgccgagtg aatccgaggt caggaagcca ccgaccggct
     2881 gccgtccttg cggtccgaag gcggcctccc aggtctcgat cacgggcatg aacgacaggc
     2941 ggactttgcc cttcctgggc gtgtccgcgc gttccgccgc ctgcgccgga agggcgccgg
     3001 ggaggaccag gacgagggcg agaacggcga aggtctttgt catgagacag aacggcaccc
     3061 ggcggtggcc gggtgccgtt ccgagtaccc caatctagtc agcccgtttc gctcagggga
     3121 tggtcttgag cgacgtgccg tacttcgcgt tgatcacggc gatcagcgtg ttcgccacga
     3181 gcttgtgggt cgcggcgctc gggtgcacgc cgtcaaacga gaacgccgtt ccgaagggag
     3241 ccgtcgtggc ccggggatcg gggggcgcgt gcgggaagaa ggcgacctgg gtcgtgtcgg
     3301 ccttcaactg cgcgagcgcc acgttcggat cgaaatacgc gtagccccgg gccgtcgcct
     3361 gcgcgctgat ataggcgttg aaagccagta cggagttgtg gagtttcaag aactccggct
     3421 tggtgatgac gaggtcgtcg gtcccgcaat tcaggacgaa gggaccgccc gtcggtgccg
     3481 cctgggccgc ggcgatgagc cccagcccat agaagaacgg cacgagagac gagtcgccga
     3541 cgccgcccag ggcggacggg gcgcagctcg cgtccacctg gaagctcggc agggctgcga
     3601 gcttggtcgc cttcagctgg aagtacacgg cgcccagcga cgcgtacgga atctgggtga
     3661 cgtccgccac gccgatcagc acgccgccct tgaccgaccc gattgcatcg atcgaatcga
     3721 gcatcgcctt gtactcggtc ttgaacgtcg ccgtatccgt gacctcgagc gaatcgcccg
     3781 gatgggtctg atcggtcgcc gcgccgagga cgtcgttgtt gccgatccac accgtgacga
     3841 aggtcggctg gaccttccgc atcatgccga tctgggtcag accacccagg aacaactggg
     3901 tgagcaggtt cgaattcgac cccgccacga ggttgttggt gggggacgat gcggtcgcgc
     3961 ccggcaccgc cacgtcgctg atatacggcg ggggcggagt ctggccttcc cggagcaggc
     4021 acgtctgcga cgtcccgccg ccgagccggg cctgtgtaaa gacgttggta tacggcggcg
     4081 cacatcccgg cttgttcaag agcggctggt agaacggcgt atgcatctgc cgcgcgacca
     4141 gcaccgcgta cgcctggcgc tgcgtgctgt cgttgatgcc gttggactgg aatcccgcgg
     4201 tgatcgaatt ccccatggac acatagcgcg tgaagagggg gctcaccggg tcttcggtgg
     4261 agttgtggaa cagctcgtcg ttgttgcagg ctgccgccgc gccgaggagg atcccgagcg
     4321 ccgccaggta ccgaatggtg gtcttggtca ttggggtctc tcgctcagaa ggtgtaggag
     4381 aggccggcgc cgaggagcac ggccttgaac gtgtagaggc cggtgttcaa ggtgctgacc
     4441 tcggacacgc gcccgcgccg atcgttctgc ttgagataca tgacactggc gtcgccgtgc
     4501 agcgcgccgg tcagctggta cccgacgccg aggatgaacg agttgcgcga gccctcgggc
     4561 aggcggggcg tcacggtctg atcgggggcc gcagccgtgt taaacacgta cccgccacgg
     4621 aacgtcagct tgggcgagta cgcgtactcg gtgccgaggc ggacaccata cgaatcccgg
     4681 tacccctcgt agtcgctcag cgggggcgtg ccggcgttgg cgaaattgac cgtgagctga
     4741 ttgaaccagc cccagacgac cgtctggagg tcacctgaca ggtgccattt gtcgttgaac
     4801 ttgtagtcga acccgatgct gccctgatcg gggagggtga cgtcggtggt cgccgcgctg
     4861 ccggacagcg gaccgccgga ggcgaattcg gatggaagca cggcgtccag cgggaggggg
     4921 ctgttgatca ggggcggagc atagagcacc agtcccgtct tgatctcctg gaagctggcg
     4981 tttcccgtga agtgaatcgt ctttcgcgtc aggaagtgtc cgccgagggt cagctgatcg
     5041 ttcaccttga agatcacgcc gccgttgaac gcgattccgt tcccgcttgc ggacaggtcc
     5101 gcattcgcga aatccgtgtt cgtcgggatg ccgacctggg cgaacgtgag gccggcgggc
     5161 agtcccgcgg gtccggtgat ggcggtcggc accaattgct gagaaagatc aaggtgctga
     5221 tgcagcgcca ccgacgcgtg gacgtacgcc acgccgaggc cgaaggagag ccgcggcgtg
     5281 acctggtacg agatggtcgg ctgaatgtag taggacttca cctgggtgtc gtatcccgcg
     5341 aaccggcccg cgaaggacaa cggccacttg gtgccgaggc cgtacggcac gtagaagccg
     5401 aggccgatcc cgatcttctc gttcaaccgg tgggtcagga agaagttcgg caccacgaag
     5461 gtctggctgg tcatgccggt gtgctgcagg aacacgtcgt cggtaaagcc gccggtgggg
     5521 ctgatcgtcg tcagtccggc actgaagtgc gttccggaaa gggacgacag gccggccggg
     5581 ttgaagaaga ccgccgagcc atccccgcag gggttggcgg cgaccgcacc gccccgcgcc
     5641 atcgtacagg tcgacagctc gtacacgcca aatccctgcg cgccaagcgc cgcggggatg
     5701 agtccgacgg cgagcaccaa cgcagtccct cgcatacgta gtgtctccgg atgctggtgg
     5761 ggagatggtg aaacaggctg gaagaaacta ggattggccc caacccttgt caaaccaagt
     5821 gatgaaagaa tcgttcacgt tgtttcgcga gacctatggc gtggccccca ccatcggtgg
     5881 atcggcgccg ggccgcgtca acctgatcgg cgagcacacc gactacaacg gcgggccggt
     5941 gctccccatg gcgatcgcgc gccggacggt cgtgatggcc ggtcccggcg accccggcgt
     6001 gatcgaggtg atctcggcgc gtgacggcga gttgcggcga gtggagtttc tccgtgaacc
     6061 gggcgagggc accacgggct tcgtggtggg agtcgcccgg tcactcgacc gcttggggat
     6121 cgctctcccg aacggcggcc gggtcgccat cacgtcggac gtgccgatcg gggcggggct
     6181 cgcgagctcc gcggcgctga cggtctccgc cgcgtgtgcg ttggccgcgc tcgcggggca
     6241 gaaactcccg gcgaaggagg cggcgcgcgt cgggtaccag gccgagcatg atcacgttgg
     6301 cgtgcgatgc gggaccatgg accagacgat cgcggcgctg ggacggaagg ggcacgccct
     6361 tctgctcgag tgcgcgaccg gcgcgacgaa acagatcccg ttccggggca cgctcctgct
     6421 cgtcgatacc ggcgtgcgtc acgaccttgc ccacagcgcg ttcaacacgc gtcgcgccga
     6481 gtgtgaggcg gcactcgaag cgctgcggaa ggtgcacccg gggttgcgct ggctcgctct
     6541 gtggcccgcg gaggagatcc cgcggctggc gcggcttctc ccttcgccgt tgcgtgcccg
     6601 ggcccagcac gtagtgggcg aatcggtgcg gacgcgggcg gccgcgatgc tgctcgcccg
     6661 gggccggctc gccaactttg gaaagctcat gtaccagtca cacgagtcgt gccgccggct
     6721 gtacgagtgc agcgcgccgg agctcgatct cgtggtacag gcggcgaagc gggccggggc
     6781 gatgggggcg agaatgacgg gggcggggtg gggtggtgcg gccctggttc tgatccggcc
     6841 aaaagcggcc gagcggaccg gaagcgcgat tgcgcgcgcg ttcgagcggc gtttcggccg
     6901 cgcgccggtc atcacgctgc tgcagggcgg ggcaggggcg cgggtagaga aggtgcgcgg
     6961 ctagattagt tatccccggg gccatgcccc ggggatagga ggatgggtgc catttcctga
     7021 ttgggcgttg ggcgtcgcgc tcatttgcgt ggcgttcttt ggtggggtgg gg
//
LOCUS       DASUBP010000070         6657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_246730, whole genome shotgun sequence.
ACCESSION   DASUBP010000070 DASUBP010000000
VERSION     DASUBP010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6657
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_246730"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..544)
                     /locus_tag="VFU75_02830"
     CDS             complement(<1..544)
                     /locus_tag="VFU75_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465794.1"
                     /translation="MGARALTLMAAALVAAACERPAAAPRRGTGDVLLRGGWIVDGAG
                     NPRYQGDLLIAGDRIAAVGRLGSAAARETLDVSGKIVAPGFIDMLGQSEMNALSDNRV
                     LSKVTQGVTTEVTGEGGSVFPLTDALVAEDSDMMKKYHLSEDWRDLDGYFRHLQKTGS
                     TINIATFVGATQLRTVVVGTG"
     gene            complement(526..1896)
                     /locus_tag="VFU75_02835"
     CDS             complement(526..1896)
                     /locus_tag="VFU75_02835"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5465795.1"
                     /translation="MNRLTYGTLAAALALLGAACTAPGKGVIALEGATLIDGSGGPPV
                     KDALILIRGGRIEAVSQVNEVHVPRGAQVINLVGKTIIPGLIDAHARSERWAMPRFVA
                     WGVTSLRDAGDSPEDTLIALRNDVALGTILGPRMYTPGSAIDAAPAGNPAWVAAAAPE
                     DARKAVDARINAGVDYVMAGPGIAPTMLRAILDEAGALKTPVALTAGRTDAASAVHMG
                     VAILDQLTGVVSAMAHNPAPIFKAYADRWAGWAAEEKGWSTFDSISVGTMARTLAGSH
                     VSLVPTLVAHDAMARLSDSSLMSGSGWADVPASRGSVRDLAGFLKETGWSTRDISAFT
                     SARTRQNQFVRDFRHAGGLVAAGSDAPSAGLAPGGSLHREMAMLVAAGLTPLEAITAA
                     TRYGAELLQADSLGVVQANRLADLVVLNSDPSQHIESTQDIAWVMLRGNIYHPDSLRL
                     QWAHEH"
     gene            complement(1893..2339)
                     /locus_tag="VFU75_02840"
     CDS             complement(1893..2339)
                     /locus_tag="VFU75_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HEU5465796.1"
                     /translation="MRLFACSLLVAAACSPARPGGDPTADITAMLNQSAASWNHGDLA
                     GFMGDYAHDTLTSYLAGGHFTHGWQPLFDHYQAAYFAPGRHRDSLTFDDVHVRVLAPA
                     VAFATAHFYLHRGDSLTASGPFTLILTHDGTRWHIVHDHTSSDPKP"
     gene            complement(2342..2602)
                     /locus_tag="VFU75_02845"
     CDS             complement(2342..2602)
                     /locus_tag="VFU75_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465797.1"
                     /translation="MLEENTMESGWSGPGMMEPLFPPPTPMAPEPAMSAEPMMAEAPA
                     PKKRATKSKKAAKKKATKGKKKAKRAKAKKPAKKGKKAKKRR"
     gene            2721..5054
                     /locus_tag="VFU75_02850"
     CDS             2721..5054
                     /locus_tag="VFU75_02850"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU5465798.1"
                     /translation="MNFRHLCISATAAVLSAVQPLAGQNVAEVQVAPAAVNIKVGERT
                     GLLATAFDRIGNVIPTAKITWTSRSPQVARVDANGTVTGTGNGVAIIEAASGRRTGLA
                     TITVSGTAPAPVAAQTPGGQPASPSGSSTAATTTAQPEQPGVDPFYGQPAGTGNATAL
                     RIVPAQIFLLPSENTKVSLRALKDDGSAAAPVRVTWSSLTPAIASIDHNGTVVALSPG
                     QGTIQVTAAAGGLTATAPVVVQQADMAIDQNGPIMMSPGDADTLHVVVPTQGNRAVSP
                     LQMQWGSSVPAVATVTLDGVLRAVGPGDAILQVQGLLQNKTVAIHVHKTVETLAVSPP
                     LAKDVEVPLAATAKFVAHPLATGGVPVPEAPLRWIVGDSTLASFDTATGMLTGLKVGK
                     TTLTVRGPGVGLQAVWNVSVIAGSVHLSASRVGLHAGQRFTIKADFTDDGGQVLGPAT
                     GLTWQTDAAGVAIVTAQGDVTAMGYGRARITATAPGGKSSSTVVFVQGELVVALDRGG
                     KNHLFSLERSSLTELRRVTNDSGPEVEPAFSSDGSRIVFVSTRDGIAQLYLMDADGTN
                     RLRLTNRQQPDSRPVFAPDGQTVVFQSTREGKKDGLEQIWSVNVDGTGLKVLTDSGGN
                     RQPSVSPDGQTIAFISNRDSKTPHVWLMNRDGTNQRAFIKGGAQRELEPHFLKDGSLA
                     FLVQRKDNGRDVTQVMKADLGSGAITPLSGTDLAIFGFGISPSGDMLALLVNAEGRRG
                     HLQLYVQPVGASGAAAAVPVPIAPNEQMLSPTFLPQP"
     gene            5068..6114
                     /locus_tag="VFU75_02855"
     CDS             5068..6114
                     /locus_tag="VFU75_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HEU5465799.1"
                     /translation="MRAAVLTDTGGLDRLTVADVPDPGSPGPGQVRVAIRAAALNHLD
                     LFVADGLPGVEYRFPHIVGADGAGVIEAVGPGVSRVKAGDRVMINPGISDRTCEFCRA
                     GEHSLCIKYRILGEHLPGTICSAIILPEFNVAAIPPVTPALSWAEAAAFSLVTLTAWR
                     MVTTRAQVKAGEWVLVWGIGGGVSLTALRIAKHLGAKVIATSSSDAKLARARELGADV
                     TLNHATQDVPKEVRALTEKRGVDVVVENVGEATWEKSIRMLAKGGRVVTCGGTTGPML
                     VTDVRRLFWGGYSILGSTMGNDAEYAAIVAELAAGRLRPVVDRVFPLEQVREAYTYLD
                     QASQLGKVVVELDR"
     gene            complement(6127..6504)
                     /locus_tag="VFU75_02860"
     CDS             complement(6127..6504)
                     /locus_tag="VFU75_02860"
                     /inference="COORDINATES: protein motif:HMM:NF017935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HEU5465800.1"
                     /translation="MPKPKLTTLVGRALRLRCPNCGRGRFFVGWFSPRKRCPECSQPL
                     ERLEDGYYLGALLLNFVGAELTVTIAGLAVLIFTWPRTPWDVLLYGGAALAVTLPFIT
                     YPFSKTVWLAIDLYIQPNEDRAT"
BASE COUNT         1013 a         2237 c         2289 g         1118 t
ORIGIN      
        1 caccggttcc gaccacgacg gtccgcaact gggtcgcgcc gacgaaggtc gcgatgttga
       61 tggtcgagcc ggtcttttgg agatgccgga agtacccgtc gagatcgcgc caatcctccg
      121 acagatgata cttcttcatc atgtcggagt cttccgccac gagggcgtcg gtgagcggaa
      181 agaccgaccc tccttcaccg gtcacctccg tcgtcacacc ctgcgtgacc ttcgacagca
      241 cccggttatc gctgagggcg ttcatctcgg actggccgag catgtcgatg aatcccggag
      301 cgacgatttt tccggagaca tcgagcgtct cccgggcggc cgcgctcccg agccttccga
      361 ccgccgcgat ccggtctccc gcgatcagga ggtcgccctg gtagcgcgga tttccggcgc
      421 catccacgat ccatccccct cgcagcagga cgtcgcccgt gccccggcgc ggggcggccg
      481 cggggcgttc gcacgcggcg gcgaccagcg ccgccgccat caaggtcagt gctcgtgcgc
      541 ccactgcagc ctcagcgagt ccgggtgata gatgttcccg cgcagcatca cccaggcgat
      601 gtcctgcgtg gattcgatgt gctgcgaggg atcggagttc agcaccacga ggtcggccag
      661 ccggttcgct tgcacgacgc ccagtgagtc ggcctgcagc aactcggcgc catatcgcgt
      721 ggcggcggtg atggcctcga gtggcgtcaa tccggctgcg accagcatcg ccatctcccg
      781 atggagtgag ccgcccggcg cgaggcccgc ggagggcgcg tcactcccgg cggcgacgag
      841 gccaccggca tgccggaagt cccggacgaa ctggttctgc cgggtccggg cgctcgtgaa
      901 ggcgctgatg tcgcgggtgg accagccggt ctccttgagg aacccggcga ggtcccggac
      961 gctcccgcgg ctcgcgggca cgtcggccca gccggacccc gacatgagcg aggagtcgct
     1021 caaccgggcc atcgcatcat gtgcgaccaa ggtggggacc agcgagacgt ggctcccggc
     1081 cagggtccgg gccatggtgc cgaccgaaat ggaatcgaac gtcgaccacc ctttctcctc
     1141 ggccgcccac cccgcccacc ggtcggcata ggccttgaag atcggtgccg ggttgtgggc
     1201 catcgccgac accacgccgg tgagctggtc gaggatggcg acgcccatgt gaaccgcgga
     1261 agccgcgtcg gtccggccgg cggtcagcgc gacaggggtc ttcagggcac cggcttcgtc
     1321 gaggatcgcc cgcagcatcg tcggcgcgat cccgggcccg gccatcacgt agtcgacccc
     1381 ggcgttgatg cgcgcatcca ccgccttgcg cgcgtcctcg ggcgccgcgg ccgcgaccca
     1441 ggcggggttc ccggcgggcg cggcgtcgat ggcggagccg ggcgtgtaca tccgcggccc
     1501 caggatggtg cccagtgcca cgtcgttgcg gagcgcgatc agggtgtctt ccggcgagtc
     1561 gccggcgtcg cgaaggctcg tgacgcccca cgcgacgaat cgcggcatcg cccaccgctc
     1621 cgagcgcgcg tgcgcatcga tcaggccggg gatgatcgtc ttgccgacga gattgatgac
     1681 ctgtgcgccg cggggcacat ggacttcgtt cacctgactg accgcctcga tgcggccgcc
     1741 gcggatgagg atcagcgcgt ccttcaccgg cggaccaccg ctgccgtcga tgagcgtggc
     1801 gccctcgagt gcgatcacgc ccttgccggg agccgtgcac gcggctccga gaagcgccag
     1861 ggccgccgcc agcgtgccgt acgtcagacg gttcacggtt tggggtccga agaagtgtga
     1921 tcgtgcacga tgtgccagcg agtgccgtcg tgcgtcagga tcaacgtgaa gggtccgctt
     1981 gcggtcagtg agtcgccccg gtggagatag aagtgggcgg tcgcgaacgc cacggccggc
     2041 gccagaacgc gcacgtggac gtcgtcgaac gtcagggagt cccgatgcct gcccggggcg
     2101 aaatacgccg cctggtagtg atcgaagagc ggctgccacc cgtgcgtgaa gtgaccgccc
     2161 gcgagatagg acgtgagggt gtcgtgcgcg tagtcaccca tgaacccggc gaggtcaccg
     2221 tggttccagc tcgccgcgga ctggttcagc atcgcggtga tgtcggcggt cgggtcgccg
     2281 ccgggccggg ctggggagca cgcggcggcg accaggaggg agcacgcgaa gagtcgcacc
     2341 gctagcggcg cttcttcgcc ttcttgccct tcttcgccgg cttcttcgct ttcgccctct
     2401 tcgccttctt cttccccttc gtggccttct tcttcgccgc cttcttgctc ttcgtcgcgc
     2461 gcttcttcgg cgccggcgct tcggccatca tcggctcggc ggacatcgcc ggttccgggg
     2521 ccatcggggt cggcggcggg aagagcggct ccatcattcc gggaccgctc cagccgctct
     2581 ccatggtgtt ctcttcgagc atcgtcggtc tccttcactg ggtgtttggc gggcgcggcc
     2641 tgtagcttac ccgggccatt gagactggca agtcggttcg gttgacaacc ccccccgcgt
     2701 gggctaacgt ttggcccgcc atgaacttcc gccacctctg catctccgcc acagccgctg
     2761 ttttgtcagc cgttcaaccc ctcgcggggc agaacgtcgc cgaggtccag gtggccccgg
     2821 cggcggtcaa catcaaggtt ggcgagcgca ccgggctcct cgcgaccgcg ttcgaccgga
     2881 tcggcaacgt catcccgacg gcgaagatca cctggacctc ccgcagcccc caggtggcgc
     2941 gggtggacgc caacggcacg gtcacgggga ccgggaacgg cgtagcgatc atcgaagcgg
     3001 cttcggggcg ccgcacgggt ctcgcgacca tcacggtctc ggggaccgcg ccggctcccg
     3061 tggcggcgca aactcccggg ggccagccgg cgtccccctc cggttcgtct accgcggcga
     3121 cgaccaccgc acaaccggag cagccgggcg tcgatccgtt ctacggtcag ccggcgggta
     3181 ccgggaacgc gaccgccctg cgaatcgtcc cggcgcagat cttcctgctc ccctccgaga
     3241 acaccaaggt cagtctccgg gcgctgaagg atgacggctc ggcggcggca ccggtacgcg
     3301 tcacctggag ttcactgacg ccggcgatcg ccagcatcga tcataacggc accgtcgtcg
     3361 cgctctcgcc cgggcaaggc accattcagg taacggcagc cgcgggtggg ctgaccgcga
     3421 ccgcgccggt ggtagtgcag caggccgaca tggccatcga tcaaaacggc cccatcatga
     3481 tgtcgcccgg ggacgccgac acgctccatg tggtggtccc gacgcagggg aatcgcgccg
     3541 tgtctccgct ccagatgcag tggggatcca gcgtgcccgc ggtcgcgacc gtcaccctcg
     3601 acggtgtgtt gcgggcggtc ggacccggtg acgccatcct tcaggtccag ggactcctcc
     3661 agaacaagac ggtggcgatc cacgtccaca agacggtgga gaccctcgcg gtgtcccccc
     3721 cgctcgcgaa ggacgtcgaa gttcctctcg ccgccaccgc gaagttcgtg gcgcaccccc
     3781 tcgcgacggg cggcgtgccc gtgccggagg cgccactccg gtggatcgtc ggggacagca
     3841 cgctcgcgtc gttcgacacc gcgacgggca tgctgaccgg gctcaaggtc ggcaagacga
     3901 cgctcaccgt tcgtggtccc ggcgtgggcc tgcaggcggt ctggaacgtc tcggtcatcg
     3961 ccggctcggt gcatctctcg gcgtcccggg tggggctgca tgccggccag cgcttcacca
     4021 tcaaagccga ctttactgac gatggcgggc aggtgctggg gccggcgacg ggcctgacct
     4081 ggcagacgga cgccgccggc gtggcgatcg tgacggcgca gggtgacgtg accgccatgg
     4141 gctacgggcg tgcgcggatc accgccaccg cgccgggggg caagagctcc tcgacggtcg
     4201 tattcgtgca gggcgagctg gtggtcgccc tcgaccgcgg gggcaagaac cacctcttct
     4261 cgctcgagcg ctcgagcctc acggagctgc gccgggtgac caacgattcg ggccccgaag
     4321 tcgaaccggc gttttcgtcg gacgggtcac ggatcgtctt cgtctcgacg cgtgacggaa
     4381 tcgcgcagct ctatctgatg gacgcggacg ggaccaaccg cctccggctc accaaccggc
     4441 agcagcccga cagccgcccg gtcttcgcgc cggacgggca gacggtcgtg ttccagtcca
     4501 cgcgggaagg gaagaaggac ggcctcgagc agatctggtc cgtcaacgtc gacggcaccg
     4561 ggctcaaggt gctcacggat tcgggtggca accggcagcc gtccgtgtcc cctgacggcc
     4621 agaccatcgc cttcatttcc aaccgggaca gcaagacccc gcacgtctgg ctgatgaatc
     4681 gcgacgggac caaccagcgc gccttcatca agggtggggc acaacgtgag ctggagccgc
     4741 acttcctcaa ggacggctct ctcgcgttcc tggtgcagcg caaggacaat gggcgcgacg
     4801 tgacccaggt gatgaaggcg gacctggggt ccggagcgat cacaccgctg tcgggcaccg
     4861 atctcgcgat tttcggtttc gggatctctc cttcgggtga catgctggcc ctgctcgtga
     4921 acgccgaggg gcgccgaggg catctgcaac tttacgtgca gcccgtcgga gccagcgggg
     4981 ctgcggccgc cgttcccgtg ccgatcgcgc ccaacgagca gatgctgtca ccgacgttcc
     5041 tgccccagcc ctaagtatct tccgcaggtg cgtgccgcag tcctcaccga caccggcggt
     5101 ctcgatcgcc tgacggtcgc cgacgttccc gacccgggct cgcctggacc gggtcaggtg
     5161 cgcgtggcca ttcgggccgc ggcgttgaat cacctcgatc tcttcgtcgc cgatggcctg
     5221 ccgggagtgg agtaccgatt cccgcacatt gtcggcgcgg acggtgccgg cgtgatcgaa
     5281 gcggtgggtc ccggcgtctc gcgggtgaag gcgggggacc gggtgatgat caatcccggt
     5341 atctcagacc gtacctgcga gttctgccgg gccggcgagc actcgctctg catcaagtac
     5401 cggatcctgg gcgaacatct gcccggcacg atctgctccg ccatcatcct tccggagttc
     5461 aatgtcgcgg ccattccgcc ggtgacgccc gcactgtcct gggccgaggc tgccgccttt
     5521 tcgctcgtga cgctgacggc atggcggatg gtcaccacgc gcgcgcaggt gaaggccggg
     5581 gagtgggtgc tggtatgggg gatcgggggc ggcgtctccc tcacggccct gcgcatcgcg
     5641 aagcacctgg gcgcgaaggt catcgcgacc tcgtcgtcgg acgccaagct tgcccgcgcc
     5701 agggagctcg gcgccgatgt cacgctcaat cacgccacgc aggatgtgcc gaaggaagtg
     5761 cgggcgctca cggagaagcg tggtgtcgat gtggtggtcg agaacgtcgg tgaagcgacc
     5821 tgggagaaat cgatccggat gctcgccaag ggcgggcgcg tggtcacgtg cggtgggacg
     5881 acgggtccga tgctggttac ggacgtccgc cggcttttct ggggtgggta ttcgattctg
     5941 ggttcgacga tggggaatga cgccgagtat gccgcgatcg tcgccgagct ggcggcgggg
     6001 cgtttgcggc cggtcgtcga ccgggtgttt ccgctggagc aggtccgcga ggcgtacact
     6061 tacctcgacc aggcttcgca gctcggcaag gtcgtcgtag agctagatcg ctaagaggct
     6121 aagacgctaa gtcgcccggt cctcattggg ttgtatgtac aggtcgatcg ccaaccacac
     6181 cgtcttcgaa aatggatagg tgatgaacgg cagggtgacc gcgagcgcgg cgcctccgta
     6241 cagcagcacg tcccagggtg tgcggggcca cgtgaagatc agcacggcga ggccggcgat
     6301 ggtgacggtc agctcggcgc cgacgaaatt gaggagaagc gcgccgaggt agtagccgtc
     6361 ttcgagccgc tcgagtggct ggctgcattc ggggcaccgt ttccgcggtg agaaccagcc
     6421 gacgaagaac cgtccgcggc cgcagttggg gcagcggaga cgcaaagcgc gaccgacgag
     6481 cgtggtgagc ttcggtttcg gcacgctcga atttaccctc ccgtaagtgc ctttcccgcc
     6541 ccggtttagg ggtcattttt tcgttgcttt tgaccacgta agtatttaga ttttttggac
     6601 ttagggcatt tccctggccc ctgactcctg acccctgaga cctgcctttc atgaccg
//
LOCUS       DASUBP010000071         2797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1336943, whole genome shotgun sequence.
ACCESSION   DASUBP010000071 DASUBP010000000
VERSION     DASUBP010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336943"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2387)
                     /locus_tag="VFU75_02865"
     CDS             complement(<1..2387)
                     /locus_tag="VFU75_02865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU5465801.1"
                     /translation="MRLTRATAIAAVTSFAAIVVAYLWAHTYAEKAWIANLAFTWCAV
                     LALGSCLRAWRTMPAEGRGAWLVMSLCCAIWLAPQLRLDYVMLVRGERSGDLTDFASL
                     SYVLSFAAYFVVYPGFFIALSNLLRDERREGFDRRVVLDIALVTLTVGGLGFEFLESS
                     YAAWLAQPVSHGTVQWLAIFGLPLMGLVVFWLILLRMIRRTGVPLATAGVAVVGVVLF
                     ASGDLSYSWQLVRNSQTSIGGPVDLLWDAGLLLVAVAAAYAASEASGRTRVNISAALS
                     RTLAVAVGLGGVIAAALLGIAFPGSFPQSSWLVLVGGTILALRVAYTVHADLQYAGRL
                     ERKAERTYVALQESEERYSAAFEQAAVGLAEVGLDGRFLRVNRCLCESLGYRADELMH
                     RSVVDITHPDDVPDSRRLLARIDTLQAPYQQEKRYLRKDGSAMWTNLTTSIVRDAEHQ
                     PKFFISVIEDLTHRKRLEQQLLHAQKLESLGQLAGGVAHDFNNVLGVMLGFTQIVERT
                     FPDEDPRRADMAQIRNAGLRAADLIGQLLAFARKSDSVPQVIDVSPLVAGLDRMLPRV
                     LGENITLRIDLEPKLPPVKVDPTQFEQVMLNLAINARDAMPEGGTLSIETDVVWLDQD
                     HVRRHPDARAGRHVMIAVSDTGTGISPEILDRIFEPFFTTKGPGRGTGLGLAMCYGII
                     KQAGGNIWAYSEPGEGTTFRLYLPTVGGEVTAAPEAARADNLPQGDEVVLIAEDDPQL
                     RQLTGRILRECGYEVLAAADGEEALELARRAGRRVEILVTDMVMPDMSGLQLADQL"
     gene            complement(2433..>2797)
                     /locus_tag="VFU75_02870"
     CDS             complement(2433..>2797)
                     /locus_tag="VFU75_02870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465802.1"
                     /translation="AVQKAADEQSRGATVRGYSSETENGQLEYEVAMTVNGRSRDVSF
                     AADGSILEIEQEVPVDSLPAAVHSALVRKAGAGRIMRVESLTKHGTLVAYEAQVRTGT
                     KRSEVQVGPDGQALPHEE"
BASE COUNT          468 a          948 c          926 g          455 t
ORIGIN      
        1 agctggtcgg ccagctgcag cccgctcatg tcgggcatca ccatgtcggt cacgagaatc
       61 tccacccggc gaccggcgcg ccgcgcgagc tcgagcgcct cttccccgtc cgcggccgcc
      121 agcacttcgt agccgcactc ccggaggatc cgcccggtca actggcggag ctgggggtcg
      181 tcctcagcaa tgagcacgac ttcgtccccc tgcggcagat tgtcggcgcg ggcggcctcg
      241 ggagcagccg tcacttcccc gcccacggtc ggcaggtaca gacggaatgt cgtcccttca
      301 ccgggttcgc tgtaggccca gatgttccca cccgcctgct tgatgattcc gtagcacatc
      361 gccaggccga ggccggtacc ccgccccggg cccttggtgg tgaagaaggg ttcgaaaatg
      421 cggtcgagga tctccggtga gattccggtc ccggtatccg agaccgcgat cattacgtgc
      481 cggccggccc gggcgtcggg atggcgccga acgtggtcct gatcgagcca tacgacgtcc
      541 gtctcgatgg acagcgtccc gccctccggc atcgcatcgc gtgcgttgat cgcgagattc
      601 agcatcacct gctcgaactg ggtggggtcg accttcaccg gcggcagttt gggctcgagg
      661 tcgatccgca gcgtgatgtt ctcacccaac acccggggca gcatccggtc gagtccggcg
      721 accagcggac tcacgtcgat cacctgcggc accgagtcac tcttgcgcgc gaacgcgagg
      781 agctgaccga tcagatcggc ggcccgcagg cccgcgttcc ggatctgggc catgtccgcg
      841 cgccgggggt cctcgtccgg gaacgtgcgc tcgacgatct gtgtgaaccc gagcatgacg
      901 ccgaggacgt tgttgaagtc gtgcgccacg ccgccggcga gctggccgag ggattcgagt
      961 ttctgggcgt gcaggagctg ctgctcgaga cgtttgcgat gggtcaggtc ttcgatgacg
     1021 gatatgaaga atttgggctg gtgctcggcg tcccggacga tcgacgtcgt gaggttggtc
     1081 cacatcgccg atccgtcttt gcgaaggtag cgcttctcct gttggtacgg cgcctggagc
     1141 gtgtcgatcc gtgccaggag ccggcgcgaa tccggaacgt cgtccgggtg ggtgatgtcg
     1201 accacactgc ggtgcatcag ctcatcggcg cggtacccca ggctctcgca gaggcatcgg
     1261 ttgaccctga ggaaccgtcc gtcgaggccg acttccgcga gaccgacggc ggcctgctcg
     1321 aaggccgcgc tgtaccgctc ttcgctctcc tgcagggcga cgtacgtccg ctccgccttc
     1381 cgctcgagcc gccccgcgta ctggaggtcg gcgtgcaccg tgtacgcgac ccgcagggcc
     1441 aggatggtac cgccgaccag gaccaaccag ctgctctggg ggaacgaacc cgggaacgcg
     1501 atgccgagga gagcggcggc gatgacaccg ccgaggccga cggcgacggc cagggtccgg
     1561 gagagggccg ctgagatgtt gacgcgggtg cgtccgctgg cctcgctcgc cgcgtacgcc
     1621 gccgcgaccg cgacgagcag cagccccgca tcccagagca ggtcgacggg tcccccgatg
     1681 ctcgtctggc tgttgcggac cagctgccag ctgtaggaca ggtcgccgga cgcgaagagc
     1741 accactccca ccacggccac gcctgccgtc gcgagcggca ctccggtccg gcgaatcatg
     1801 cgaaggagaa tcagccagaa taccacgagg cccatcagcg gcagaccgaa gatggcgagc
     1861 cattgtacgg tgccgtggga gacgggctga gccagccagg ccgcatagga ggattcgagg
     1921 aactcgaacc caaggccccc gaccgtaagg gtgaccagcg cgatgtcgag caccacacgc
     1981 cggtcgaacc cctcccgccg ttcgtcgcgg agcaggttgc tcagcgcgat gaagaatccg
     2041 ggatagacga cgaaataggc ggcgaacgag aggacgtagg agagcgaggc gaagtccgtc
     2101 agatcacccg accgctcacc ccgcacgagc atgacgtagt cgagacgaag ctgcggcgcc
     2161 agccagatcg cacagcacag actcatcacc agccaggcgc cacgtccctc ggccggcatg
     2221 gtccgccagg ctctgaggca gctcccgagc gcgagcacgg cgcaccacgt gaacgccagg
     2281 ttcgcgatcc atgccttctc ggcgtaggta tgcgcccaga ggtaggccac aacaatcgcc
     2341 gcgaacgagg tgaccgcggc gatggctgtg gctcgagtga ggcgcattgg tactagtcta
     2401 cggaccggcg ggaaggctgc aagcgtatgg ggtcactctt cgtgcgggag cgcctgtccg
     2461 tcaggcccta cttgcacctc ggagcgcttc gtgcccgtgc gcacctgtgc ttcataggcg
     2521 accaaggtcc cgtgcttcgt cagggattcc actctcatga tccgtccggc gccggccttc
     2581 cgcaccagtg ccgaatgcac ggcggccggg agcgaatcca ccgggacttc ctgctcgatt
     2641 tccagaattg acccgtccgc cgcgaagctc acgtccctgc tccggccgtt cacggtcatg
     2701 gcgacctcgt actcgagctg accgttctcg gtttcgctcg agtaccctcg gaccgtcgca
     2761 cccctgctct gttcatcggc ggctttttgc accgccg
//
LOCUS       DASUBP010000072         7462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_464911, whole genome shotgun sequence.
ACCESSION   DASUBP010000072 DASUBP010000000
VERSION     DASUBP010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7462
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_464911"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2111
                     /locus_tag="VFU75_02875"
     CDS             <1..2111
                     /locus_tag="VFU75_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU5465803.1"
                     /translation="VGSGGMNGAFGLPGFTWIDGGKRYSYIKREQRSEEIRAFDPATG
                     KDTLVFSAQGLTFPDSSAAFTYNEFQWTADSRNLVFETNFRPIYRRSGIQDFYVYSVA
                     DHKLKLATRDARTAELSPDGSMLGLERGGNMYVYDFAAKRETQLTKDGAELVYNGHYD
                     WVYEEEFGQAQAWNWSPDSRHIAYWQVDERSEPVVQFSDYQGLHQDWTKIRIPQPGDS
                     NPTVKIGVVDVKSGANTWLNPGLAGDFYIPRIYWTSRPDTLALVTLNRQQDTMRLFFF
                     DVKTGGKRLVLTKTSNTWIDVYDFYAGVNDLMTFPAGRSEFYWVGDEDGWQHIYRYDY
                     SGRLINQVTKGKWIVARIEGMDPARGLIYYTSTEVSPLERQLYEIHVDGSGKKRLTTT
                     AGNHHINMSPDTRYYTDRWSSMTSPHQVELWATGGKMVKSLAGNESTKTWLASHEFSP
                     PELFSFRTSDSVQLDGSMIKPIPFDATRKYPVVFDIYGGPGSQQVYNAWQANGWDEWL
                     AEQGYIVIGLNNRPSNNYGSAFMKVAYKHLGKYEAHDFAEAAKYLATLPYVDSKHVAI
                     IGTSYGGFSTVFTMEMYPDLFPVGVANSAVADWRLYDTIYTERYMGLLGDNQAGYVES
                     SPIEQAAKLTGHLLLVHSMMDDNVHPQNTMQLITALDEAGHPAPLMMFPKGHHGAAYD
                     FPTFIDIQEATFAWLERYMK"
     gene            2256..3521
                     /locus_tag="VFU75_02880"
     CDS             2256..3521
                     /locus_tag="VFU75_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465804.1"
                     /translation="MRNFRAAPFILFAAALSVTACDNSSMTGTKTNPSGPNVFADQIS
                     LGGFTTILSSGTSRVSIRVIPGTATASRVRVRQGDQVNRSERIVSEVASLDTTGTIVL
                     SLGGISVSFDATTKFEGWHDDMDSNDSAAMGEAGFISRLEAALAAGHHPTIVALRAAP
                     ATPQSPTDGSFHADVLRLNDDADRPTIEMNVTSANLASNTTPPPDAFLTVLNVAIGLD
                     VTGGKTRIDANTQNTMGAIHFEGRVQSVDTTGGTATLADSAGTVIKVPTGGEIEHPDD
                     HDEDFDNPLNSLSRVAAALAAGDTVDAAGFGLKAGTDTIDAVEVRFRVRNNHEFEPTV
                     VGFEGNVSSADSTTGKVVLGNGTTIDVTDSTHFITMDGGLANLGAAVRALGDTSLRVR
                     AEGVGKPISTAEIAAFFIRIEADSGGEHH"
     gene            3783..4721
                     /locus_tag="VFU75_02885"
     CDS             3783..4721
                     /locus_tag="VFU75_02885"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5465805.1"
                     /translation="MAAPRLALLETGRLTYERARLQEALSHRYDIVRELGQGGMSRVF
                     LGWDRLEGRRVALKVLRAGLSASVEDRERFRREALIASRLNHPNIVPCYDFILRRETA
                     VAVMRYVPGDSLSSRLAGGLRLGPATTVRLLAPIADALSLMHQRGVVHRDVKPANILL
                     HADDQWPFITDFGIATLRTSEHSRAEATRRFGTPEFMSPEQALGSWDADHRSDVYSLG
                     LVAYLALAGELPFRGGSPMAQIAQRATLDVPPVLDRAPDVPERLAAAIDRALNRSPSR
                     RWPTAEAFRQALVDSLDTPTWPERFGRWVKRSLRRR"
     gene            complement(4718..5200)
                     /locus_tag="VFU75_02890"
     CDS             complement(4718..5200)
                     /locus_tag="VFU75_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465806.1"
                     /translation="MIASLALLQALLQGPVDTTAGFRNHAYFEMGAAVGATSPFGGSG
                     LAVGLHVTSALPNAAGIDFSIASYIDPLTHGNLTMLTDLDAAYLATGPSRPSVVLRGG
                     LTTVIAHGMALGLNAGAGVMVPVGKTISLRADYTFRGLLGDLTGITLSGLTVGVGVDY
                     "
     gene            complement(5197..5649)
                     /locus_tag="VFU75_02895"
     CDS             complement(5197..5649)
                     /locus_tag="VFU75_02895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465807.1"
                     /translation="MKWIAVGVAILVAGCSGVGSGLGSPNARAWKPGANHADYTVARD
                     SAPGTWLLRGSWDFAIGPDSVISGTWRSHWLVADTLAQIGPQVGSGVLGGRLQSDGTL
                     SIDLNPDLVDNNVWVRVGPREGAEATWTWSTIAGPWTGGPFRLDPPAF"
     gene            complement(5891..7279)
                     /locus_tag="VFU75_02900"
     CDS             complement(5891..7279)
                     /locus_tag="VFU75_02900"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5465808.1"
                     /translation="MTRALACLTTALIATTLSGQQPSLVSTYGADADRIIAAAQSDSS
                     AWRRLAELTDTFGSRIAGSTNLEQAIDWVLAEMKKDGFANVHGEPVMVPHWVRGAESA
                     ELVSPRRVRLHMLGLGNSVGTPPAGITAPVLVVTSFDDLQKHAGEAKGKIVLYDVAFP
                     QEGDPFFNYGKVVAYRVAGAIQAAKVGAVASLIRSVASGSMQSPHTGVMVYDSTTRRI
                     PHAALSVEDAEMLHRMQDRGQKVVLTLKMQAHTLPEAHSRNVVAELVGREKPDEVVVL
                     GGHIDSWDVGEGAMDDGGGAVAAWEAVKLLQRLGLHPRRTVRVVLWTTEEFGGVGGQA
                     YAKDHASDLPKHVLGLESDNGTFDPRGFMYNGTPANLPFVTQLDSLLAGLGNFHSDTG
                     FAETDVAPMGAHGVPLLGLKVDPSRYFFYHHTDADMMDKIDPRELARCVAAMAVAVYV
                     AADMTESLAHGQ"
     gene            complement(7276..>7462)
                     /locus_tag="VFU75_02905"
     CDS             complement(7276..>7462)
                     /locus_tag="VFU75_02905"
                     /inference="COORDINATES: protein motif:HMM:NF019590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HEU5465809.1"
                     /translation="WGIVAFDSLALYEAYRTRVKADPEARANFAFAHDRRLILREERT
                     FLRMVDGIASRPTPEAP"
BASE COUNT         1362 a         2506 c         2342 g         1252 t
ORIGIN      
        1 tggtggggag cggcggaatg aacggggcgt tcggcctccc gggcttcacc tggatcgacg
       61 gcgggaagcg ctattcctac atcaagcgcg aacagcggtc cgaggaaatc cgggccttcg
      121 acccggcgac cggcaaggat acgctcgtct tcagcgccca gggcctgacc ttccccgatt
      181 cgagcgcggc gttcacctac aatgagttcc agtggaccgc cgactccagg aacctggtgt
      241 tcgagaccaa cttccgtccg atctaccgcc ggtccggcat ccaggacttc tacgtctact
      301 cggtggctga ccacaagctc aagctcgcga cccgggacgc tcgcaccgcc gagctgtccc
      361 ctgacggttc gatgctcggc ctcgagcgcg gcggcaacat gtacgtgtac gacttcgccg
      421 cgaagcgcga gacgcagctg accaaggacg gggcggagct ggtatacaac gggcactacg
      481 actgggttta tgaggaggag ttcggtcagg cccaggcctg gaactggtcc ccggactcgc
      541 gccacatcgc gtactggcag gtggatgagc gttcggagcc ggtggtgcag ttctcggatt
      601 accagggcct gcaccaggac tggacgaaga tccgcattcc gcagcccggc gattcgaacc
      661 ccacggtgaa gatcggcgtg gtggacgtga agtccggcgc caacacgtgg ctcaatcccg
      721 gtctcgccgg cgacttctac attccgcgca tctactggac ctcgaggccg gacaccctgg
      781 ccctcgtaac cctgaaccgc cagcaggaca ccatgcggct cttcttcttc gacgtgaaga
      841 cgggcggcaa gcggctggta ctgacgaaga cctcgaacac ctggatcgac gtctacgatt
      901 tctacgccgg cgtcaacgat ctgatgacgt tcccggcggg ccggtccgag ttctactggg
      961 tgggagacga agacggctgg cagcacatct accgctacga ctactccggc cgcctcatca
     1021 atcaggtcac gaagggcaaa tggatcgtcg cgcgcatcga agggatggac ccggcgcgcg
     1081 gcctgatcta ctacacgtcc accgaggtct cgccgctgga gcgccagctc tacgaaatcc
     1141 acgtggacgg cagcggcaag aagcggctga cgaccaccgc cggcaatcac cacatcaaca
     1201 tgtccccgga cacccggtac tacacggacc ggtggtcctc gatgacctcg ccgcatcaag
     1261 tggagctctg ggcgacgggc gggaaaatgg tgaagtccct cgccgggaat gagagtacga
     1321 agacctggct ggcgtcgcac gagttctcac cgccggagct gttctcgttc aggacttcgg
     1381 actcggtcca gctcgacgga tcgatgatca aacccatccc gttcgacgcg accaggaagt
     1441 atcccgtggt gttcgacatc tacggtggac ccggctctca gcaggtctac aacgcctggc
     1501 aggccaacgg ctgggatgaa tggctcgccg aacaaggcta catcgtcatc ggcctgaaca
     1561 accggccgtc gaacaattac ggcagcgcgt tcatgaaggt ggcgtacaag catctcggga
     1621 agtacgaggc ccatgatttc gccgaggccg cgaagtatct cgcgacgctc ccctacgtgg
     1681 actcgaagca cgtcgccatc atcggcacga gctacggcgg cttcagcacg gtcttcacga
     1741 tggagatgta cccggacctc ttcccggtgg gcgtggccaa ttccgcggtg gccgactggc
     1801 ggctctacga caccatttac accgagcgct acatgggcct gctgggggac aaccaggcgg
     1861 gctacgtgga gagctcaccc atcgagcagg cggccaagct caccggccac ttgctgctgg
     1921 tgcactcgat gatggacgac aacgtccatc cgcagaacac catgcagctc atcacggccc
     1981 tggacgaagc gggacacccg gcgcccctga tgatgttccc caaggggcac cacggcgcgg
     2041 cgtatgactt cccgaccttc atagacatcc aggaggcgac gttcgcgtgg ctcgaacgct
     2101 acatgaagta gtttgaccca cgacggtgtg acgtaacgca caagtcgttg gaccataccg
     2161 atttcgaacg ctccgcccca gtgacgaata ggggaattgc ggggtattct tggcgagcgt
     2221 cccaagtgcc aagaaacccc gcaggaggag ctcccatgcg taatttccgc gcggcgccgt
     2281 tcatactctt cgccgccgcc ttgtccgtca ccgcttgcga caacagcagc atgaccggaa
     2341 ccaagacgaa ccccagtggc cccaacgttt tcgcagatca gatcagtctc ggcggtttca
     2401 ccaccatcct ctccagtggc acctcacgcg tctcgatccg ggtgatccca ggaacggcga
     2461 cggccagtcg cgtgcgcgta cggcagggtg atcaggtcaa ccgttcagag cgaatcgtca
     2521 gtgaggtcgc gtcgctcgac accaccggca cgattgtatt gtcactcggc ggcatcagcg
     2581 tctcgttcga cgccacgacc aagttcgaag gctggcacga cgacatggat tccaacgact
     2641 ccgccgcgat gggcgaagcc ggtttcatca gccgtctcga ggccgcgctc gccgcggggc
     2701 accacccgac gatcgtggcg ctccgggccg cccccgcgac tccgcagtct cccaccgacg
     2761 gctcgttcca cgccgatgtg ttgcggctca acgatgacgc cgatcgtccc accatcgaaa
     2821 tgaacgtgac gtcggcgaat ctcgcgagca ataccacgcc tcccccggat gccttcctca
     2881 ccgtgctcaa cgtcgccatt ggactcgatg tcaccggtgg caagacccgg atcgatgcga
     2941 atacgcagaa cacgatgggc gcgatccatt ttgaagggcg cgtgcagagc gtcgatacga
     3001 cgggtgggac cgcgactctc gcggacagcg ccggcaccgt catcaaggtc ccgacgggtg
     3061 gggagatcga gcaccccgac gaccacgacg aggacttcga caacccgctg aactccctct
     3121 cccgggtcgc ggctgccctg gcggccggtg acaccgtcga tgccgcgggc ttcggcctca
     3181 aagccggcac cgacacgatc gacgccgtcg aggtccggtt ccgcgttcgt aacaatcacg
     3241 aattcgagcc gacggtcgtc gggttcgaag gaaacgtgtc gtccgccgac agcacgacgg
     3301 ggaaagtcgt gctcggcaat gggacgacga tcgacgtgac cgactccacg cacttcatca
     3361 ccatggacgg cggactcgcc aacctcggtg cggcggtgag ggcgctcggc gacacctcgc
     3421 tccgcgtgcg cgccgagggc gtcggcaaac cgatcagcac ggccgaaatc gcggcgttct
     3481 tcatccgcat cgaggccgat tcgggcggcg agcaccacta gacagtctcg cctggcttgt
     3541 tctcagggga gtccctcacg ggactccctt ttttttcgca acgctccacg ccctcacggc
     3601 atctaaggga gatgagggga tgagtcttcg gatgtccggt tttgggactt gcagtcccgc
     3661 gaccggtaca acgcccgccc gagcatggtg tcccatcacg ttgtgatata tgcagttgca
     3721 gcgccgccga aaacggcaca gagcgtgcct tagtacgaat cgaaatcctc caagggacgg
     3781 ttgtggcggc accacggctc gcgttgctcg aaactggacg actcacgtac gagcgtgccc
     3841 ggctccagga agcgctgagc caccggtacg acatcgtgcg cgagttgggt cagggcggga
     3901 tgagccgggt gtttctgggg tgggaccgcc tcgagggcag acgtgtcgcg ctgaaggtgc
     3961 tgcgcgccgg cttgtcggcc tcagtggaag atcgcgagcg ctttcgccgc gaggcgctca
     4021 tcgcgagccg cctcaatcat ccgaatatcg tcccctgtta tgacttcata ctccggcgcg
     4081 agaccgcggt ggcggtgatg cggtacgtgc ccggagattc actttcgtcg cggcttgccg
     4141 ggggcctgcg cctcggcccc gcgacgaccg tcagactgtt ggcacccatc gccgacgcgt
     4201 tgtccctcat gcaccagcgt ggcgtggtcc accgggacgt gaagccggcc aacatcctgc
     4261 tccatgccga cgatcagtgg cccttcatca ccgatttcgg gatcgcgacc ctgcgcacct
     4321 ccgagcactc gcgcgccgag gcgaccagac ggttcggaac acccgagttc atgtcgccgg
     4381 aacaagcgct cggttcgtgg gacgccgacc accgcagtga cgtgtattcg ctgggtctgg
     4441 tggcgtacct cgcgctggcc ggggagttgc ccttccgcgg cggttccccg atggcccaga
     4501 tcgcacagcg ggcgaccctg gacgttccgc ccgtcctcga ccgcgcgccc gatgtgccgg
     4561 agcgcctcgc ggccgcgatc gaccgcgcgc tcaatcggag cccgtcccgg cgatggccga
     4621 cggcggaggc gttccggcag gcgctcgtcg actccctcga cacgccaacg tggcccgagc
     4681 gattcggtcg ctgggtcaag cggagccttc gccgtcgcta gtagtcgact ccgacgccga
     4741 ccgtcaaacc ggacagggtg atgccggtga gatccccgag cagtccgcgg aaggtgtagt
     4801 cagcccgcag ggagatggtc tttcccaccg gcaccatcac accggccccg gcgttcaaac
     4861 ccagggccat cccatgtgct atcacggtgg tgagaccgcc cctcagcacc acgctgggcc
     4921 gtgaaggtcc cgtcgccagg taggccgcgt cgagatcggt gagcatggtc aggtttccgt
     4981 gcgtcagcgg atcgatgtag ctcgcgatcg agaaatcgat acccgcggcg ttcggcaggg
     5041 ccgatgtgac gtggagtccg accgccagtc cggatccgcc gaagggtgaa gtggcgccca
     5101 cagcggcgcc catctcgaaa taggcatgat tccggaagcc ggcggtcgtg tcgaccggcc
     5161 cctgaagcag agcctggagc agtgcgagag atgcgatcaa aaggcgggag gatcgagtcg
     5221 gaaggggcca cccgtccacg gaccggcaat ggtcgaccag gtccacgtcg cttcggcgcc
     5281 ctccctcggc cccacccgaa cccagacgtt gttgtccacg agatccggat tcagatcgat
     5341 cgacaaggta ccgtcactct ggagccgccc gcccaacacg cctgacccca cctgcggtcc
     5401 gatctgcgcg agggtatccg caaccagcca gtgagatctc caggtcccgc tgattacgga
     5461 atccggcccg atcgcgaagt cccaggaacc ccgcagcagc cacgtccccg gcgcactgtc
     5521 tctcgcgacg gtgtagtcgg cgtgatttgc tcctggcttc caagctcggg cgtttgggct
     5581 tccaaggccc gagccgaccc ccgaacaccc ggccaccagt atggcgaccc caacggcgat
     5641 ccatttcatg ggttacctcc gttccaaaga gatacaccac ttctccccgg gaggcacccc
     5701 gcctgtcggc gagtcgcggg atcaacgcgg tcggtgggtc ggcccgtcag ttcgactgga
     5761 aggtcagccg gagatcggtc gctctcgatc gggagcgcgg gagccattcc agctgtaccg
     5821 tcccattcgg gaccggaagg cgcgcggcag ggcccccagt tcgagttgct ggaccccggg
     5881 ctaaggaaat tcactgtccg tgagccagcg attccgtcat gtcggcggcg acgtaaacgg
     5941 ctacggccat cgccgcgacg caccgagcca gctcccgcgg gtcgatcttg tccatcatgt
     6001 cagcatcggt gtgatggtag aagaagtacc gggacggatc gaccttgagc cccagaagcg
     6061 gcacgccgtg cgcccccatg ggcgccacgt ccgtctcggc aaatccggtg tcgctatgga
     6121 aattgcccag ccccgcgagc agtgagtcga gctgagtcac gaacggcagg ttcgcggggg
     6181 tcccgttgta catgaagccg cgtggatcga aagtgccgtt gtctgactcg agtcccagca
     6241 cgtgcttcgg caagtccgag gcgtgatcct tggcgtaggc ctggccgccc accccaccaa
     6301 attcttccgt ggtccacagc accacccgaa ccgtccgccg gggatggagc cccagccgct
     6361 gcaggagctt caccgcctcc cacgcggcca ccgcaccgcc gccgtcatcc atcgcccctt
     6421 cgcccacatc ccatgaatcg atatggccgc cgaggaccac cacctcgtcg ggcttctcgc
     6481 gtccgacgag ctccgcgacg acgttgcgcg agtgggcctc cggcagggtg tgcgcctgca
     6541 tcttgagggt cagcacgacc ttctgtccgc ggtcctgcat ccggtgcagc atctccgcat
     6601 cttcgacgga gagcgcggcg tgcgggatcc ttcgggtcgt cgagtcgtag accatcaccc
     6661 ccgtgtgggg actctgcatg gagcctgagg ccaccgagcg gatcaagctc gcgaccgcgc
     6721 ccacctttgc cgcctggatg gcccccgcga cgcggtaggc cacgaccttc ccatagttga
     6781 agaagggatc cccctcctgc gggaaggcaa cgtcgtagag gacgatcttg cccttcgcct
     6841 cgccggcgtg tttctggagg tcatcgaagc tcgtcaccac cagcaccggc gcggtgatgc
     6901 cggcgggagg agtaccgacg ctgtttccga gtcccaacat gtgcagacgg acacgccggg
     6961 gagagaccaa ttccgcggac tcggctcccc gcacccaatg cggcaccatc accggttccc
     7021 cgtggacgtt cgcgaacccg tccttcttca tctcggccag cacccagtcg atcgcctgct
     7081 cgaggttggt cgagccggcg atgcggctgc cgaaggtgtc ggtaagctcg gccaggcgtc
     7141 gccaggcgct cgagtcgctc tgcgcggcgg cgatgattcg gtcggcgtcg gcgccgtagg
     7201 tggacaccag cgagggctgc tgccccgaca gggtcgtggc gatgagagcc gtcgtcaggc
     7261 aggcgagtgc gcgcgtcatg gagcctccgg agtgggcctt gaagctatcc cgtccaccat
     7321 ccgaaggaat gtccgttcct cgcgcagaat cagccgtcgg tcgtgcgcaa acgcgaaatt
     7381 ggcgcgggcc tcaggatccg ctttgacccg ggtgcggtag gcctcataga gcgccaacga
     7441 atcgaaggcg acgatgcccc ag
//
LOCUS       DASUBP010000073         3465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1105502, whole genome shotgun sequence.
ACCESSION   DASUBP010000073 DASUBP010000000
VERSION     DASUBP010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3465
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1105502"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..838
                     /locus_tag="VFU75_02910"
     CDS             65..838
                     /locus_tag="VFU75_02910"
                     /inference="COORDINATES: protein motif:HMM:NF015272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HEU5465810.1"
                     /translation="MAVTYSSYLKLDELLALQKPLSEGPEHDEMLFIVIHQVYELWFK
                     ELLHELDYLQKLLRESDQPRAGHTFKRILTILKVLVAQIDILETMTPLEFLSFRDRLE
                     SGSGFQSHQFRELECVLGSKNPKSLDRYPSGSAARKAVEQRLHDPTLWDAFLACLNRN
                     GHQVPERRVFTAAIEPSPAVQEVLIRVYRKDPSVASLCERMVDLDEGLQEWRYRHVKM
                     VQRTIGTRRGTGGSAGAEYLATTLNRPAFPDLWEIRTEL"
     gene            835..1998
                     /locus_tag="VFU75_02915"
     CDS             835..1998
                     /locus_tag="VFU75_02915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465811.1"
                     /translation="MIDPKSLTRHYSRFRVTERVLLTGHSHQAWPDHGFEGQARAWTD
                     AAELVDDKWERAFAMAGAVRRGFARLLHDEADQIALGASTHDLLVRFLSGLPLKAKRR
                     LVTTDGEFHSLRRQLDRLGEEGVAIVRVPAHPVASLAERVRKEVTANTAAVLLSAVLF
                     ETAEIVPGLGSIAAACRSAGVEFLVDAYHAINVIPFPVPTLGLTGAYILGGGYKYCQM
                     GEGNCFLRFPSDTTMRPVVTGWYSEFEHLESKSTGKVQYGSGPARFAGSTYDPTSHYR
                     AAEVLRFFSDQQLTPERLRELNKRQISRLAHGFDELALDPKVITRVVHGLDGIAGFLA
                     LRTPSAIDFAKALRTRGVYADARHDILRLGPAPYVTDDQLEQALKALAETVRA"
     gene            2056..2298
                     /locus_tag="VFU75_02920"
     CDS             2056..2298
                     /locus_tag="VFU75_02920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465812.1"
                     /translation="MNNLEKTPKVEVTETKHEQEPTPTWQELRDRFRAVLTDLWDETR
                     DIERELEPKVLPALKRLRDEVAKLIEKIEGRTKKPG"
     gene            complement(2295..2723)
                     /gene="tadA"
                     /locus_tag="VFU75_02925"
     CDS             complement(2295..2723)
                     /gene="tadA"
                     /locus_tag="VFU75_02925"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008251 - tRNA-specific adenosine
                     deaminase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0002100 - tRNA wobble adenosine to inosine
                     editing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEU5465813.1"
                     /translation="MNHAIDWARLAQSVDEVPVGAALLQHGQLVAGAHNETIGLKDPT
                     AHAELLTIQRSLRALDTDRLSDCTLYITLEPCAQCAGAIVLAKVGRVVFGAYDDKAGM
                     AGSVEDLLRHPRLNHRPEVIGGVDAETCGTLLKEFFASRR"
     gene            complement(2734..>3465)
                     /locus_tag="VFU75_02930"
     CDS             complement(2734..>3465)
                     /locus_tag="VFU75_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5465814.1"
                     /translation="LFDDERAMVRLDMSEYMEKHAVARMIGAPPGYVGYEEGGQLTEA
                     IRRRPYAVVLFDEIEKAHPDVFNVLLQILDEGRLTDSQGRTVDFRNTVLIMTSNIGSQ
                     DILEMGTANWEQVEAKVLDLLRRSFKPEFLNRVDDTIIFRPLSEADIAKIVDVQLKRF
                     ERLLEERKLTLDVTPAAKQLLVSEGYDPIYGARPLKRSMQRLLQNPLAMAVLEGTYVE
                     GDTIKADRGTDGALTFEKVPGGRRK"
BASE COUNT          598 a         1188 c         1086 g          593 t
ORIGIN      
        1 ggctatcgct tacgggtgaa gtgctcccta gtttgtgggc acatctccca cgcggaatcc
       61 tcggatggct gtgacgtatt cgagctacct gaaactggat gagctgctcg cgctccagaa
      121 gcctctttcc gaggggccgg agcatgacga gatgctcttc atcgtgatcc atcaggtcta
      181 cgagctctgg ttcaaggagc tcctccacga gctcgactac ctccagaaac tcctccggga
      241 gagcgaccag ccccgcgccg gacacacctt caagcggatc ctcaccatcc tcaaggtcct
      301 ggtcgcccag atcgatattc tcgaaaccat gacgccgctc gagttcctgt cgttccggga
      361 ccgtctggag tcggggagcg gcttccaatc gcaccagttc cgtgagctcg agtgtgtcct
      421 cggctccaag aatccgaagt ccctcgaccg gtatccgtcc ggaagcgcgg cgcggaaagc
      481 cgtcgagcaa cggcttcacg atcccacgct gtgggatgcc tttctcgcct gcctgaaccg
      541 gaacggacac caggtccccg agcgccgggt cttcaccgcg gcgatcgaac cgagccccgc
      601 cgtgcaggaa gtcctgatcc gggtctaccg gaaggacccc agcgtggcct cgctctgcga
      661 gcgcatggtc gacctcgacg aaggcctgca ggagtggcgc taccggcacg tgaagatggt
      721 ccagcgcacc atcggcaccc ggcgcggcac cggaggctcg gcgggcgccg agtacctggc
      781 gaccacgctc aaccggccgg cattccccga cctctgggaa attcgcaccg agctgtgatc
      841 gacccgaaat ctctcacccg tcactactcg cgattccgcg tcacggagcg cgtgctcctc
      901 accggacact cacaccaggc ctggcctgac cacggcttcg agggccaggc gcgagcctgg
      961 accgacgccg ccgagctggt ggacgacaaa tgggagcggg cgttcgcgat ggccggtgcg
     1021 gtccgtcgcg gcttcgcgcg cctcctgcac gacgaagcgg accagatcgc cctcggcgcc
     1081 tcgacccacg atttgctggt ccggttcctt tccgggcttc ccttgaaggc gaaacgacgg
     1141 ctcgtcacca ccgacggtga gttccattcg cttcggcggc agctcgatcg tctgggagag
     1201 gaaggcgtcg cgatcgtgcg ggtccccgcc catccggtcg cgagcctggc ggaacgggtg
     1261 cgcaaggagg tcacggcgaa cacggccgcg gtactcctct ccgccgtgct cttcgagacc
     1321 gccgagatcg tgccgggtct cgggagcatc gcggccgcct gccgcagcgc tggggtagag
     1381 ttcctggtcg acgcgtatca cgccatcaac gtcattccat tccccgtccc gactctcggc
     1441 ctcaccggcg cgtacatcct cggcggcggt tacaagtact gtcagatggg agaaggcaac
     1501 tgtttcttgc ggttcccctc cgacaccacc atgcgcccgg tggtcaccgg gtggtacagc
     1561 gagttcgagc acctcgaatc gaaatccacc ggcaaggtgc aatacggcag cggccccgcg
     1621 cgcttcgcgg gctcgaccta cgacccgacg agtcattacc gcgccgccga ggtgctccgg
     1681 ttcttcagcg accagcagct gacgccggag cgcctgcgcg agctcaacaa gcggcagatc
     1741 agccgtctcg cgcatggctt cgatgagctg gcgctcgatc ccaaggtcat cacgcgggtc
     1801 gtccatggac tcgacggcat cgcgggattc ctcgccctgc ggacgccctc ggcgatcgat
     1861 ttcgcgaaag cccttcggac caggggcgtc tatgcggacg cccggcacga catcctgcgc
     1921 ctcggtcctg cgccctatgt caccgacgat cagctcgagc aggcgctcaa ggcgctcgcg
     1981 gagaccgtgc gggcctgact ccgaaactcc cccagcctga ttggcgtaaa agtctcctaa
     2041 cggcaaggag acgccatgaa caacctcgag aagacaccga aggtcgaagt caccgagacc
     2101 aagcacgagc aggagccgac gcctacctgg caggagctgc gggaccgttt ccgcgccgtg
     2161 ctcacagacc tctgggatga gacccgggac atcgagcggg agctcgagcc caaggttctg
     2221 cctgcgctga aacggttgcg ggacgaggtc gcgaaactga tcgagaagat cgaaggaaga
     2281 accaagaagc ccggctaacg ccgggatgcg aagaactcct tcaggagggt cccgcaggtc
     2341 tcggcatcca ccccgccgat gacctcgggc cggtgattca gccggggatg gcgcagcagg
     2401 tcttcgaccg agcccgccat gcccgctttg tcgtcgtagg ccccgaacac cacccgcccg
     2461 accttcgcca gcacgatcgc gcccgcgcac tgggcgcagg gctcgagcgt gatgtacagg
     2521 gtgcagtcgc tgagccggtc ggtgtcgagg gcccggagcg accgctggat cgtcagcagc
     2581 tccgcgtgcg ccgtcgggtc cttgaggccg atggtctcgt tgtgggcgcc cgccacgagc
     2641 tggccatgct ggaggagcgc cgctcctacg ggcacttcgt cgaccgactg cgcgagccgc
     2701 gcccagtcga tcgcgtgatt catgccccgc cggtcacttt cgtcgaccac ccggtacctt
     2761 ttcgaacgtc agtgctccgt cggtgccgcg atcggccttg atcgtgtccc cctcgacgta
     2821 cgttccctcg agcaccgcca tggccagcgg gttctgcagc agacgctgca tcgaccgctt
     2881 gagcggacgg gcgccataga tcggatcgta gccctcggat accaggagct gtttggcggc
     2941 cggcgtcacg tcgagtgtga gcttgcgctc ctccaggagc cgctcgaacc gcttgagctg
     3001 gacgtccacg atcttcgcga tgtcggcctc ggacaggggc cggaagatga tcgtgtcgtc
     3061 cacccggttc aggaattccg gcttgaacga ccggcgcagg aggtcgagga ccttggcctc
     3121 gacctgctcc cagttcgccg tccccatctc caggatgtcc tgcgagccga tgttggacgt
     3181 catgatcaac acggtatttc ggaagtcgac ggtccgcccc tgggagtccg tgaggcgccc
     3241 ttcgtccaga atctggagca gcacgttgaa gacgtccgga tgtgccttct cgatctcgtc
     3301 gaaaagcacc acggcgtagg gacgccggcg gatggcctcc gtgagctggc caccctcttc
     3361 gtagccgaca tagcccgggg gcgcgccgat catccgggcc acggcgtgct tctccatgta
     3421 ctccgacatg tcgagacgga ccatcgcccg ttcgtcgtcg aacag
//
LOCUS       DASUBP010000074         3077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_138140, whole genome shotgun sequence.
ACCESSION   DASUBP010000074 DASUBP010000000
VERSION     DASUBP010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3077
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138140"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1400)
                     /locus_tag="VFU75_02935"
     CDS             complement(<1..1400)
                     /locus_tag="VFU75_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5465815.1"
                     /translation="MTLTTVAKIFETMDWGPAPEARGPALEWIKAHDGRFGLYVGGRW
                     VAPSAGDGFDTINPATREPLARIGQAGTEDVDAAVKAASEALPAWAGLSGHARARHLY
                     ALARTVQKHSRLLAVLESLDNGKSIRETRDLDIPLVARHFYHHAGWAQIRDTEFPGYG
                     PVGVVGQIIPWNFPLLMVAWKVAPALAAGNTIVLKPAEFTPLTALCFAELAHEAGLPA
                     GVLNVITGDGRTGKLLVDHPGVDKIAFTGSTEVGRQIRKATAGSGKKLTLELGGKSPF
                     IVFEDADLDSVVEGVVDAIWFNQGQVCCAGSRLLVQEGVAGKLIPKLRARMEKLRVGE
                     PLDKAIDMGAIVAPVQLERIRSLVDQGVAEGATMWQPTWACPTDGYFYPPTLFTDVAP
                     AASIAQVEIFGPVLVSMTFRTPAEAVELANNTPYGLASSVWTENINLALDIAPKIKAG
                     VVWINSTNLFDAAAGFG"
     gene            complement(1397..2308)
                     /gene="deoC"
                     /locus_tag="VFU75_02940"
     CDS             complement(1397..2308)
                     /gene="deoC"
                     /locus_tag="VFU75_02940"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEU5465816.1"
                     /translation="MISSIRVNRSAVERRAATLPKRRTVKKEWQAAWLLRAITCIDLT
                     TLSGDDTAGNVSRLSAKAREPIRRDLLEALGASDLPIQVGAVCVYHALVPVAVKALAG
                     SGIPVAAVSTGFPAGLSPLEERVHEVRASVDAGAREIDVVITRGHVLTGAWERLYDEV
                     WRFREACGAAHMKVILGTGELATLTNVARASRVAMAAGADFIKTSTGKEPVNATLPVG
                     LVMVRQIRDYLEQTGYAVGFKPAGGIRSAKGALEWLMLMKEELGERWMRSDRFRLGAS
                     ALLTDIERQLEHFVTGRYSAAHRHPMV"
     gene            2533..>3077
                     /locus_tag="VFU75_02945"
     CDS             2533..>3077
                     /locus_tag="VFU75_02945"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU5465817.1"
                     /translation="MPTPPERVIVVADAHLGQVPPATAAAFHRFLTAVPQPSDHLLIN
                     GDLFDFWFEYGAVIPRKHFGHIAALRELRQRGVRITLMGGNHDRWGGPFLTDDLGIEF
                     HADPVEMRLAGRPTFVAHGDGLTEQHWSGALTHRILKHQWTISVFRALHPDLGFWIAD
                     KLSGTLADNTKDRAVLDRAALA"
BASE COUNT          498 a         1059 c         1005 g          515 t
ORIGIN      
        1 ccgaacccgg cggccgcgtc gaacagattg gtcgaattga tccagaccac tccggccttg
       61 atcttcggtg cgatgtcgag cgcaagattg atgttctcgg tccagacgct cgacgcgagc
      121 ccgtacggcg tgttgttcgc gagctccacc gcctcggccg gggtccggaa cgtcatcgaa
      181 accaggacgg gaccgaagat ctccacctgc gcgatgctcg cggctggtgc gacgtcggtg
      241 aagagggtcg ggggatagaa gtagccgtcg gtgggacagg cccacgtcgg ctgccacatc
      301 gtggcgcctt cggccacgcc ctgatccacc agggaccgga tgcgctcgag ctggacgggc
      361 gcgacgatgg cgcccatgtc gatcgccttg tcgaggggct caccgacgcg aagcttctcc
      421 atgcgcgcgc gcagcttggg gatgagtttt cccgccacgc cctcctgtac cagaaggcgc
      481 gaaccggcgc aacacacctg gccctgattg aaccagattg cgtcgacgac gccctcgacc
      541 acgctgtcga gatcggcgtc ttcaaaaacg atgaacgggc ttttcccgcc gagctcgagt
      601 gtaagcttct tgcccgaccc cgccgtggcc ttgcggattt gccgtcccac ttcggtggat
      661 ccggtgaacg cgatcttgtc gactccggga tgatcgacga gaagcttccc cgtgcgcccg
      721 tcaccggtga tgacgttgag gaccccggcc gggagtcccg cctcgtgtgc cagctccgcg
      781 aagcagagcg ccgtgagggg cgtgaattcc gcgggcttga gcacgatggt gttccccgcc
      841 gccaacgcgg gcgcgacttt ccacgcgacc atcaggagcg ggaaattcca gggaatgatc
      901 tgccccacga cgccgacggg cccgtacccc gggaactcgg tgtcccggat ctgcgcccac
      961 cccgcgtggt gatagaaatg gcgtgcgacc agcggaatat cgagatcgcg cgtctcgcgg
     1021 atggactttc cgttgtcgag gctctccagc acggcgagca aacgggaatg cttctggacc
     1081 gtccgggcga gcgcgtagag gtgcctcgcc cgggcgtgcc cggagagccc cgcccacgcc
     1141 ggcaaggcct cgctcgccgc tttcaccgcc gcgtcgacgt cttcggtccc ggcctgcccg
     1201 atccgggcga ggggctcgcg cgtggccggg ttgatggtgt cgaacccgtc cccggcgctg
     1261 ggagcgaccc atcgtccgcc gacgtagagt ccgaagcgcc cgtcatgcgc cttgatccat
     1321 tccagcgcag gcccccgcgc ctccggcgcc gggccccaat ccatggtttc gaaaatcttg
     1381 gcgacggtag tcagggtcac accatcggat ggcggtgagc cgccgagtag cggccggtga
     1441 cgaaatgctc gagctggcgc tcgatatccg tgagcagcgc gctggcgccc aggcgaaaac
     1501 ggtcggagcg catccagcgc tcacccagct cttccttcat caacatcagc cactcgagag
     1561 cacccttcgc actgcggata ccgcccgcgg gcttgaagcc gacggcgtac cccgtctgct
     1621 cgaggtagtc acggatctga cggaccatga ccaatcccac cgggagcgtc gcgttgacgg
     1681 gctccttgcc ggtggacgtc ttgatgaagt cggctccggc ggccatcgcc actcgcgatg
     1741 cccgggccac gttcgtgagc gtcgccagct ccccggtgcc gagaatgacc ttcatgtgtg
     1801 ccgcgccgca ggcttcgcgg aaccgccaca cttcgtcata gagccgctcc caggcgccgg
     1861 tcaacacgtg acccctggtg atcacgacgt cgatttccct ggcccccgca tcgaccgagg
     1921 ccctcacttc atggacccgc tcttcgaggg gtgagaggcc cgccgggaat ccggtcgaca
     1981 cggccgcgac cgggatccct gatcccgcca acgccttgac cgcgacgggt accagcgcgt
     2041 ggtagacgca cacggctccg acctggatgg gcaggtccga tgcgccgagc gcttcgagga
     2101 ggtcgcggcg gatcggctcc cgggccttgg cggacaggcg ggacacgttc cctgccgtgt
     2161 cgtcccccga cagggtcgtc aggtcgatgc aagtgatggc ccggagcagc caggctgcct
     2221 gccactcctt cttgacggtc cggcgcttcg ggagggtggc agcccgccgc tcgaccgcgc
     2281 tgcggtttac ccggatcgag ctgatcaact caagatcaag gggaatcccc gggttccggg
     2341 caggctccgc cggcggctgg gacggacgat ccccccggga caccctcccc ggggggttcg
     2401 tgggaccagg ccgctcatac ttcgtggggt cgcgggtcac cttcatacgg gccgagatat
     2461 ggaggtgaca cgagcaaaag tcaaatagga caggagcttg cgaggtcggg acccgtttcc
     2521 tagcttgggg ccgtgccgac cccaccggaa cgcgtcatcg tggtggccga tgcgcacctc
     2581 ggccaggtcc cacccgccac ggccgccgca tttcaccggt ttctcaccgc ggtccctcag
     2641 ccgtctgacc acctgctcat caacggcgat ctcttcgatt tctggttcga atacggcgcc
     2701 gtcattcccc gcaagcactt cgggcacatc gccgcgctgc gggagcttcg gcagcggggg
     2761 gttcggatca cgctgatggg ggggaaccac gaccgctggg gagggccctt tctcaccgac
     2821 gacctgggaa tcgagttcca cgcggatccc gtcgagatgc ggttggccgg aaggcccacc
     2881 ttcgtggccc acggcgacgg tctgaccgag cagcattgga gcggcgccct cacccaccgc
     2941 atcctgaagc atcagtggac catttcggtg tttcgcgcgc tccaccccga cctgggattc
     3001 tggatcgcgg acaagctgtc ggggacgctc gcggacaata ccaaagaccg tgcggtgctc
     3061 gatcgcgcgg ccctggc
//
LOCUS       DASUBP010000075        13439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_492197, whole genome shotgun sequence.
ACCESSION   DASUBP010000075 DASUBP010000000
VERSION     DASUBP010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 13439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13439
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492197"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..349
                     /gene="rpsJ"
                     /locus_tag="VFU75_02950"
     CDS             41..349
                     /gene="rpsJ"
                     /locus_tag="VFU75_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEU5465818.1"
                     /translation="MAGKIRIRLKGFDHALIDQAAADIVRTAEKTGAQVSGPIPLPTK
                     RQLWTVLRSPHIDKKSREQFELKTHKRLIDILDSRSQTVDALTKLDLPAGVDVEIKVE
                     "
     gene            358..978
                     /gene="rplC"
                     /locus_tag="VFU75_02955"
     CDS             358..978
                     /gene="rplC"
                     /locus_tag="VFU75_02955"
                     /inference="COORDINATES: protein motif:HMM:TIGR03625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEU5465819.1"
                     /translation="MNGLIGRKLGMTRLFNEEGAHVPVTVIEAGPCAVVQVSGTQAQL
                     GFGAKKAKRSTKAMLGHAKAAGVETQPRRIESFAVGAEPPKAGEAVTVAIFAVGDRIK
                     ITGVTKGRGFQGVVHRHHFGGGPATHGNTRHRKPGSIAPGTDPSRIIKGKRMPGHMGA
                     RRFTELGLIVVRVDAERNLLFVRGSVPGPMNGLLTVRKQGGRSRHD"
     gene            971..1606
                     /gene="rplD"
                     /locus_tag="VFU75_02960"
     CDS             971..1606
                     /gene="rplD"
                     /locus_tag="VFU75_02960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEU5465820.1"
                     /translation="MIEAPHYSAAGAKQTKAVALPEALFDGTVNEPVLHQAVITFLAN
                     QRQGTHQTKTRHFVTGGNQKPWRQKGTGRARQGSSRAPHWRHGGIAFGPHPRDYRMGI
                     PKKVRQLAKKSALNARARENALLVVDTLTYEKPSTKSFAQLLGKLGIGEKKVLMLTAG
                     APTAHNVYLSGRNIPTVRVLPFADATAYEILWSDTVVVELPALSGTEHADV"
     gene            1599..1886
                     /gene="rplW"
                     /locus_tag="VFU75_02965"
     CDS             1599..1886
                     /gene="rplW"
                     /locus_tag="VFU75_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEU5465821.1"
                     /translation="MSDHLTTIVRAVVTEKSSAAYQARKEYAFEVRKDATKPEIKSAI
                     EHLFKVNVTDVRTLVVRAKRRTYGRTQGRRPAWKKAIVTLREGDAIQVFEG"
     gene            1888..2727
                     /gene="rplB"
                     /locus_tag="VFU75_02970"
     CDS             1888..2727
                     /gene="rplB"
                     /locus_tag="VFU75_02970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEU5465822.1"
                     /translation="MGIRQFKPVTPGTRFRSVADFSDITKSTPEKSLLEPLRRTGGRN
                     NKGHVTMRHQGGGHKRQYRRIDFRRDKVGVPARVAAVEYDPNRNARIALLHYVDGEKR
                     YILHPKGMNVGDTVVSGPGSDIRIGNAMPLFEIPLGTAVHNIELKIGRGGQMCRGAGT
                     SAQVVAKEGNHVTLRLRSTEMRMVRGECLATVGEVGNAEHELLSRGKAGKTRWLGWRS
                     KVRGVAMNPVDHPLGGGEGKSSGGRPPTSPWGKPEGKKTRHKKKASTKLIVRGRKRGK
                     ATQ"
     gene            2727..3044
                     /gene="rpsS"
                     /locus_tag="VFU75_02975"
     CDS             2727..3044
                     /gene="rpsS"
                     /locus_tag="VFU75_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEU5465823.1"
                     /translation="MARSVKKGPYVEESLLLKIQALNEKNEKKVFKTWSRRSTITPDF
                     VGHTLAVHNGNKFIPVYVTENMVGHKLGEFAATRLFRGHSAKGSAEKEAEAGTPPAEE
                     GKK"
     gene            3048..3383
                     /gene="rplV"
                     /locus_tag="VFU75_02980"
                     /pseudo
     CDS             3048..3383
                     /gene="rplV"
                     /locus_tag="VFU75_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010896322.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
     gene            3537..4196
                     /gene="rpsC"
                     /locus_tag="VFU75_02985"
     CDS             3537..4196
                     /gene="rpsC"
                     /locus_tag="VFU75_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEU5465824.1"
                     /translation="MGQKTHPYGFRLGIVKQWRSRYYARHDFPELLKEDELIRKYLKT
                     RLSHAAIADIHIERKPGKVTVTVHTGRPGVVIGKRGAEVDKLRDELAQLTGKEVGINV
                     EEIKRPELDAQLVGDSVVHQLNQRISFRRAMKRAVQSAMRMGAQGIKIKCAGRLGGAE
                     IARTEGYHEGRVPLHTLRADVDYATSTAKTTYGTIGVKVWIFKGERTEDLHGRTYSTG
                     V"
     gene            4200..4613
                     /gene="rplP"
                     /locus_tag="VFU75_02990"
     CDS             4200..4613
                     /gene="rplP"
                     /locus_tag="VFU75_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEU5465825.1"
                     /translation="MLAPKRVKFRKRFKGNTNGNATRGNTVAFGHYGLMSLDPGWITN
                     RQIEAARVALTREMKRGGKVWIRLFPDKPITKKPAETRMGKGKGNPEGWVAVVKPGRV
                     MFEVEGIEEKLAREALELAAAKLPLRTKFVKREER"
     gene            4615..4818
                     /gene="rpmC"
                     /locus_tag="VFU75_02995"
     CDS             4615..4818
                     /gene="rpmC"
                     /locus_tag="VFU75_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEU5465826.1"
                     /translation="MAKQGKPGDLRELKTEELEQQLVQLREERFRLKFRSATEAIENP
                     LQFRTIRRHIARIRTILRERQSA"
     gene            4815..5066
                     /gene="rpsQ"
                     /locus_tag="VFU75_03000"
     CDS             4815..5066
                     /gene="rpsQ"
                     /locus_tag="VFU75_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003022794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEU5465827.1"
                     /translation="MTRGNRKVRTGVVVSDKMQKTVTVSLVRRYAHGVYGKQMTRTKT
                     VAAHDEQGAKAGDTVRIEETRPLSKTKRWRVVEIVTRAQ"
     gene            5082..5450
                     /gene="rplN"
                     /locus_tag="VFU75_03005"
     CDS             5082..5450
                     /gene="rplN"
                     /locus_tag="VFU75_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEU5465828.1"
                     /translation="MVQQESRLKIADNSGARSALVIRVLGGSKRRYAGLGDIVVVTIK
                     DAIPTGQVKKGEVAKAVIVRTAKETKRKDGSYIRFDENAAVLINDQGEPRATRIFGPV
                     GRELREKRFMKIVSLAPEVI"
     gene            5452..5838
                     /gene="rplX"
                     /locus_tag="VFU75_03010"
     CDS             5452..5838
                     /gene="rplX"
                     /locus_tag="VFU75_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEU5465829.1"
                     /translation="MRRLVYRKSRKIRGTEPERHKLHIAKGDTVRVIRGDDRGKEGKV
                     LHVHPKVHRVTVEGVNVVKKHQRATTPETESGIIEFPAPISAANVMLLDPKSGEPTRV
                     RRQRDKDGTIERIAVKSGQPIPRARQ"
     gene            5838..6506
                     /gene="rplE"
                     /locus_tag="VFU75_03015"
     CDS             5838..6506
                     /gene="rplE"
                     /locus_tag="VFU75_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEU5465830.1"
                     /translation="MADTQEKQPKQKKEKKEKKAAPETQAQEAPSGEPAPRARLLDHY
                     EQKVRGKLQQQFGYVNQHQIPKLVKIVLNVGIGDASKNPKTLEAAVAELEAITGQKAM
                     VTKAKKAIANFNLRAGVPIGCAVTLRGPRMFEFLDRFINVAVPRMRDFRGLPDKSFDG
                     RGNYTLGIKEQMIFPEIDFDKVEKIHGMDITFVTTAGRDDAALALLREMGMPFRGEPP
                     VVAA"
     gene            6520..6705
                     /locus_tag="VFU75_03020"
     CDS             6520..6705
                     /locus_tag="VFU75_03020"
                     /inference="COORDINATES: protein motif:HMM:NF005974.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEU5465831.1"
                     /translation="MAKKCWIEKVKRTPKFKVRAYYRCQRCGRSRGTLRKFKLCRICF
                     RELALRGEIPGVRKASW"
     gene            6721..7116
                     /gene="rpsH"
                     /locus_tag="VFU75_03025"
     CDS             6721..7116
                     /gene="rpsH"
                     /locus_tag="VFU75_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEU5465832.1"
                     /translation="MSMTDPVADMLTRVRNACMAGHRRVDMPVSKLKAEIARLLRDNA
                     YIQDFKVLDDGKHGLLRLYLKYHNDSPVIRELQRVSAPGLRRYVKAREIPRIKNGLGM
                     AILSTPQGLMTDREARTARVGGELLAVVW"
     gene            7125..7670
                     /gene="rplF"
                     /locus_tag="VFU75_03030"
     CDS             7125..7670
                     /gene="rplF"
                     /locus_tag="VFU75_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEU5465833.1"
                     /translation="MSRIGKKPVPLPKGVTATVAGHALTVKGPKGEISRTFHTDMALA
                     VEAENVVVKRPSDEARHKALHGLSRTLVANMVEGVTKGFQKQLEIQGVGYKAEPKPFG
                     VQLVVGFSHPVPYHAPKGIKITVDNNVNVKVEGVDKELVGQVAAELRAVKPPEPYKGK
                     GIRYQGEQVRRKAGKTAQAAK"
     gene            7673..8044
                     /gene="rplR"
                     /locus_tag="VFU75_03035"
     CDS             7673..8044
                     /gene="rplR"
                     /locus_tag="VFU75_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEU5465834.1"
                     /translation="MRSIRKVKSANHQRLRRHLRIRKALTGTAERPRLVVFRSAKHIY
                     AQVVDDTKGVTLVGASDRTEGVQPEGKGKTAKSFALGRHIAAKAKEKGIARVVFDRGG
                     YQYHGRVKAVADGARKGGLEF"
     gene            8296..8793
                     /gene="rpsE"
                     /locus_tag="VFU75_03040"
     CDS             8296..8793
                     /gene="rpsE"
                     /locus_tag="VFU75_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016196837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEU5465835.1"
                     /translation="MVENVVAINRVAKVVKGGRRFSFNALVAVGDGQGHVGVATGKAN
                     EVSEAVRKAVEAARRKMSEIPLTVGTIPHEIVGHHGAGRVLLKPAASGTGVIAGGPVR
                     AVLECVGIRDILTKSLGSTNPHNMVRATMDGLKRLTTARRVARERGVPDDAIHYKARQ
                     EVTHV"
     gene            8786..9052
                     /gene="rpmD"
                     /locus_tag="VFU75_03045"
     CDS             8786..9052
                     /gene="rpmD"
                     /locus_tag="VFU75_03045"
                     /inference="COORDINATES: protein motif:HMM:TIGR01308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEU5465836.1"
                     /translation="MSRLPPTRKYRKRKPGRDPVLPPVGKGKLRIEQVRSTSGHPFPI
                     RRTLEALGLKHHQDVVIQKDHPALRGMLYHVRHLIKVTPAKDGA"
     gene            9125..9562
                     /gene="rplO"
                     /locus_tag="VFU75_03050"
     CDS             9125..9562
                     /gene="rplO"
                     /locus_tag="VFU75_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEU5465837.1"
                     /translation="MRLHHLKPAPGSTQARRRVGRGPGSGLGKTSGKGNKGEKARSGG
                     GRRPGFEGGQMPLIRRIPKRGFTNPFKQPAQVVNVRHLSFLKDGLEVTPETLFGAGLV
                     RRPDHPIKLLGMGDVARKFTVKGVSVSTSAKGKIEAAGGIIEG"
     gene            9569..10879
                     /gene="secY"
                     /locus_tag="VFU75_03055"
     CDS             9569..10879
                     /gene="secY"
                     /locus_tag="VFU75_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEU5465838.1"
                     /translation="MTAPAIPNLFKVPELKNKILFTLLCLVIYRVGAHIATPGVNVQA
                     LSDALQQAGKGNLFGLYDLFAGGGLGRATVFALGIMPYISASIVFQMAGPVFPILEKM
                     QRDEEGRKKITQWTRYLTVVLCFLQAYGYALFVETQVPGAVLNPGMGFRLTTVLTLTT
                     GGIFVMWLGEQITERGIGNGASLLIFFSIIERIWPEFINTFDSIKAGTASVKLILFVI
                     VMMVMITGATVAITVAARKIPIQIPRKVMGRGRIREGQKTFIPLRLLTANVMPIIFAQ
                     SLIIIPSTLATFVGGSLLRQVNDYFSAGSALYIVSLLVLILFFTYFYTSIVFNPVDLA
                     ENLKKQGGFIPGVKPGAATADYIDEVLSRITLPGGFFLALCAIIPIIAANGLNLPFNF
                     GGTALIIVVGVALDTIQQVQQHMLLRHYDGFMKEGRVKFRGRTR"
     gene            10903..11466
                     /locus_tag="VFU75_03060"
     CDS             10903..11466
                     /locus_tag="VFU75_03060"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF011100.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEU5465839.1"
                     /translation="MFILLLGAPGSGKGTQGKLLSERLGLPKITTGDLIRTAIKTGTP
                     LGVEAKKFYDAGKLVPDSVIMGLIQEQLAKPEAKEGAILDGFPRTAAQAELVDRTLAT
                     TQHRLNHVLLLDVPEEELVSRMLGRARQEGRADDTPEAIKTRLGVYQVETAPLVAHFA
                     QRGIVHRVPGTGTVDQIAEEIKRIIGR"
     gene            11463..12224
                     /gene="map"
                     /locus_tag="VFU75_03065"
     CDS             11463..12224
                     /gene="map"
                     /locus_tag="VFU75_03065"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEU5465840.1"
                     /translation="MITIKSGREIETMAEAGKIVAKTLALVAANVRPGVSTQQLDELA
                     EEFIRSHPGAKPSFKGLYDYPATLCTSINQEVVHGIPSSKRVLKEGDLVSVDVGAWLD
                     GLHADSAATFPVGVIPEAAQRLLDVTKKALAAGIAEARAGNHTGDIGAAVQQVAEGAG
                     YSVVRELVGHGIGSAFHEEPQVPNYGKPKRGPRLVAGMTIAIEPMVNAGGADIRTLPD
                     KWTVVTEDGSLSAHFEHTVAVGPEGTPPRILTTAA"
     gene            complement(12349..12495)
                     /locus_tag="VFU75_03070"
     CDS             complement(12349..12495)
                     /locus_tag="VFU75_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465841.1"
                     /translation="MSSLETRNEARGVDTREVVQPNHRILITGDWTEDAIGDRAGFDP
                     DLLE"
     gene            12715..13248
                     /locus_tag="VFU75_03075"
     CDS             12715..13248
                     /locus_tag="VFU75_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465842.1"
                     /translation="MTLTRNQAFLLAGATTASFTIYHLSHAVTREELRDIRGMYRVAL
                     CGQSTAEAEGDSTPRVANLAGYWRLSGPTVPGRLVATFTQDGNQLRVNIVAKDQCGRD
                     LLTVDLTLLGEVDADTVRFWSGSRSFEGYLGNACALYAEFQEKVKFEGVLTADGKSFE
                     GRYDDTGLPTHVWRFSR"
BASE COUNT         2540 a         4201 c         4692 g         2006 t
ORIGIN      
        1 ggcgtcgtca ccaagatcag gaagtaacgg agaagtccca atggccggaa agatccggat
       61 tcgtctcaag ggtttcgacc acgcgctgat cgatcaggcc gccgcggaca tcgtccgcac
      121 ggccgagaag accggtgcgc aggtgtcggg ccccatccca ctgcccacca agcggcagct
      181 gtggacggtg ctccggagcc cgcatatcga caagaagagc cgcgagcagt tcgagctcaa
      241 gacgcacaag cggctcatcg acatcctcga ctcgcggtcg cagacggtgg acgcgctgac
      301 caagctcgat ctgccggcgg gtgtggatgt agagatcaag gtcgagtaga ggaagccatg
      361 aacggactta tcggaagaaa actggggatg acgcgcctct tcaatgagga aggcgcgcac
      421 gtgcccgtga ctgtgatcga agccggcccc tgtgccgtgg tgcaggtgtc cggcacccag
      481 gcgcagctcg gcttcggggc caagaaggcc aagcggtcca ccaaggcgat gctggggcac
      541 gcgaaggccg cgggtgtcga gacccagccc cgccggatcg agagcttcgc ggtcggcgcc
      601 gagcctccca aggcgggcga ggccgtgacg gtcgcgatct tcgcggtcgg ggaccggatc
      661 aagatcaccg gcgtcaccaa gggccgcgga ttccagggcg tcgtgcaccg ccatcacttc
      721 ggcggcggcc cggccaccca cggcaacacg cggcaccgga agcccggctc catcgcgccg
      781 ggtaccgatc cgtcgcggat catcaaaggc aagcggatgc cgggccacat gggcgcgcgc
      841 cgcttcaccg agctcggcct catcgtcgtc cgggtggacg ccgagcgcaa tctcctcttt
      901 gtgcgcggct cggtgccggg accgatgaac ggtctgctca ccgtccggaa gcagggaggc
      961 cggagccgcc atgattgagg ccccgcacta ctcggccgcc ggcgcgaagc agacgaaggc
     1021 tgtcgcgctg ccggaagcgc tcttcgacgg gaccgtgaac gagccggtgc tgcaccaggc
     1081 cgtgatcacg ttcctggcga accagcgtca gggcacgcat cagaccaaga cgcggcactt
     1141 cgtgaccggt gggaaccaga agccgtggcg gcagaagggg accggccgcg cccgtcaggg
     1201 ctcgagccgc gctccgcact ggcggcatgg cggcatcgcc ttcgggcctc atccccggga
     1261 ctaccggatg gggattccga agaaggtgcg gcagttggcc aagaagtcgg cgctgaacgc
     1321 gcgggcgcgc gagaacgcgc tgctcgtggt ggatacgctc acctacgaga agccgagcac
     1381 caagtcgttc gcgcagctcc tgggcaagct cggcatcggg gagaagaagg tgctgatgct
     1441 gaccgcgggg gcgccgacgg cgcacaacgt ctacctctcg ggccgcaaca tcccgacggt
     1501 acgcgtgctg ccgttcgccg acgccacggc gtacgagatt ctctggtcgg acacggtggt
     1561 ggtggagctg ccggccctga gcgggacgga gcacgccgat gtctgatcat ctgacgacca
     1621 tcgtccgcgc ggtggtgacc gagaagtcgt cggccgccta ccaggcgcgg aaggaatacg
     1681 cgttcgaggt gcggaaagac gcgaccaagc cggagatcaa gtcggcgatc gagcacctct
     1741 tcaaggtgaa cgtgaccgac gtccggacac tggtcgtgcg ggccaagcgc cgcacctacg
     1801 gacggacgca gggccgccgg cccgcgtgga agaaggcgat cgtgaccctc cgtgagggcg
     1861 acgcgatcca ggtcttcgag ggctgatatg ggcattcgtc aattcaagcc ggtgacgccc
     1921 ggcacgcgct tccgctcggt ggcggatttc tccgacatca ccaagagcac tccggagaag
     1981 tccctgctcg agccgctccg ccggaccggc ggacggaaca acaagggcca cgtcaccatg
     2041 cggcatcagg gtggcgggca caagcggcag taccggcgga tcgacttccg gcgcgacaag
     2101 gtcggggtgc cggcccgtgt cgccgcggtg gaatacgatc cgaaccggaa cgcgcgcatc
     2161 gccctgctcc attacgtgga cggggagaag cggtacatcc tgcaccccaa gggcatgaac
     2221 gtgggggaca cggtggtgtc ggggccgggt tcggacatcc gcatcggcaa cgcgatgccg
     2281 ctgttcgaga ttccgctggg cactgcggtg cacaacatcg agctgaagat cggccgcggc
     2341 ggccagatgt gccgcggggc cgggaccagc gcgcaggtgg tcgcgaagga aggcaaccac
     2401 gtcaccctcc gcctccggtc cacggaaatg cggatggtgc gcggcgagtg cctggcgacg
     2461 gtgggtgagg tcggcaacgc cgagcacgag ctgctctcca gggggaaagc gggcaagacc
     2521 cgctggctgg gttggcgctc gaaggtccgt ggcgtggcga tgaatccggt cgaccacccg
     2581 ctgggcggcg gcgaaggcaa gagctccggc gggcgtcccc cgacgtctcc ctggggcaag
     2641 ccggaaggca agaagactcg gcacaagaag aaggcgtcca cgaaactcat cgtgcgcggc
     2701 cggaagcgcg ggaaggcgac tcagtaatgg cgcggagcgt caagaagggc ccgtacgtcg
     2761 aggagtcgct gctcctcaag atccaggccc tgaacgagaa gaacgagaag aaggtcttca
     2821 agacctggtc gcggcgcagc accatcacgc cggacttcgt ggggcacacg ctcgcggtgc
     2881 acaacgggaa caagttcatc ccggtgtacg tgaccgaaaa catggtgggc cacaagctcg
     2941 gcgagttcgc ggcgacgcgg cttttccggg gacacagcgc caagggttcg gcggagaagg
     3001 aagccgaagc gggtacgccg ccggccgaag aagggaagaa gtaagtcatg gaagctcgag
     3061 cgattcagac ggcggtccgc cagtcggcgc ggaagatgcg cctggtcatc gaccagatcc
     3121 ggggccgggc ggtcccggag gcggatgcca tcctgcggtt ttcgaagaag acggcggcgg
     3181 tccagatccg caaagtcctc aagtcggcgg tggccaacgc cgagcgctcg gccgagcggc
     3241 agaacgagcg gctggacgtg gacacgctgc gggtccgcta tgccgtggtg aacgagggtc
     3301 cgactctcaa gcggttcacc atggcggcga tgggccgcgg cacgccgatc aagaagcgga
     3361 cgagccacgt ggagattcgc gtgggcgcgg atgcggtggc aagcaagccg gtgcctgctc
     3421 cgaaggcaga gaccaaggcg aagacgaagg cggttaaccc cgcggccaag ccgcgggggg
     3481 aagcgaaggc caaggcggaa aagccggcga agaagccccg gcgcaaggag aagtaaatgg
     3541 gtcagaagac acatccctac gggttccggc tcgggatcgt caagcagtgg cgttcccggt
     3601 actacgcgcg gcatgacttc ccggagctgc tcaaggaaga cgagctgatc cggaagtacc
     3661 tgaagacccg gttgtcgcac gccgccatcg ccgacatcca catcgagcgg aagcccggca
     3721 aggtgaccgt cacggtgcat accggacgtc ccggcgtggt gatcggcaag cgcggcgcgg
     3781 aagtggacaa gctccgcgac gagctggcgc agctgaccgg caaggaagtc gggatcaacg
     3841 tggaggagat caagcgtccc gagctcgacg cccagctggt cggtgacagc gtggtgcatc
     3901 agctcaatca gcgcatctcg ttccgccgcg ccatgaagcg cgcggtgcag agcgcgatgc
     3961 ggatgggcgc gcaggggatc aagatcaagt gcgcgggccg cctgggcggg gctgaaatcg
     4021 cgcgcaccga aggctatcac gaggggcgcg tgccactgca cacgctccgg gccgacgtgg
     4081 attacgccac ttcgaccgcg aagaccacct acggcaccat cggcgtcaaa gtgtggatct
     4141 tcaagggcga gcggacggaa gatctgcacg gccggaccta ttcgacgggc gtctgagcga
     4201 tgctggcacc caagcgcgtc aagttccgga agcggttcaa gggcaacacc aacggcaacg
     4261 ccacccgcgg caacaccgtg gcgttcggcc actacggcct catgtcgctc gatcccggct
     4321 ggatcaccaa ccggcagatc gaagcggccc gtgtggcgct cacccgcgag atgaagcgcg
     4381 gcggcaaggt gtggatccgc ctcttcccgg acaagccgat caccaagaag ccggccgaaa
     4441 cccggatggg aaaagggaag ggcaatcccg aggggtgggt cgcggtcgtg aagccgggcc
     4501 gggtgatgtt cgaagtcgag gggatcgagg aaaagctggc ccgcgaagca ctggagctcg
     4561 cggcggccaa gctgcccctc cggaccaagt tcgtgaagcg ggaggagcgg taagatggcc
     4621 aagcagggaa agccgggcga cttgcgggag ctcaagaccg aggagctgga gcagcagctg
     4681 gtgcagcttc gcgaggagcg cttccggttg aagttccggt cggccaccga ggccatcgag
     4741 aacccgctgc agttcaggac gatccggcgt cacatcgcgc ggatcaggac cattctgcgc
     4801 gaaaggcaga gcgcatgacg aggggcaacc gaaaggtccg caccggggtg gtggtgagcg
     4861 acaagatgca gaagaccgtg acggtctccc tggtccgtcg ctacgcccac ggcgtctacg
     4921 gcaagcagat gacccggacc aagaccgtgg ccgctcacga cgagcagggt gccaaggcgg
     4981 gcgacaccgt ccgcatcgaa gagacgcgcc cgctctccaa gaccaagcgc tggcgcgtgg
     5041 tcgaaatcgt cacacgcgcc cagtagccgg gagagacgac cgtggttcaa caggaatcga
     5101 ggctcaagat cgcggacaac tccggcgcgc gcagcgcgct ggtgatccgc gtgctgggcg
     5161 gatccaagcg ccgttacgcg gggctcgggg acatcgtggt ggtgaccatc aaggacgcca
     5221 tccccaccgg ccaggtgaag aagggcgaag tcgccaaggc cgtgatcgtc cggaccgcca
     5281 aggaaaccaa gcggaaagac ggatcctaca tccgcttcga cgagaatgcc gccgtgctga
     5341 tcaacgatca gggcgagccg cgcgccaccc gcatcttcgg ccccgtgggc cgcgagctgc
     5401 gggaaaagcg gttcatgaag atcgtgtcgc tggcgccgga ggtcatctga tatgagaagg
     5461 ctggtctacc ggaagagccg gaagattcgc ggcaccgagc ccgagcgtca caagctccat
     5521 atcgccaagg gcgatacggt gcgcgtgatc cggggcgacg accgcggcaa ggaaggcaag
     5581 gtgctccacg ttcaccccaa ggtgcaccgg gtgacggtgg agggcgtcaa cgtcgtgaag
     5641 aagcatcaac gcgcgacgac gccggagacg gagagcggca tcatcgagtt cccggcgccg
     5701 atctcggccg cgaacgtgat gctcctggac cccaagtcgg gtgagcccac acgggtgcgc
     5761 cggcagcggg acaaggacgg aaccatcgag cggatcgcgg tgaagtcggg ccagccgatt
     5821 ccgcgcgcga ggcaataatg gcggacaccc aggaaaagca gccgaagcag aagaaagaga
     5881 agaaggagaa gaaggccgct ccggagaccc aggcgcagga agcgccctcc ggggagccgg
     5941 cgccccgtgc gcgtctgctc gaccactacg agcagaaggt ccgcggcaag ctccagcagc
     6001 agttcggcta cgtgaaccag caccagatcc cgaagctggt caagatcgtg ctgaacgtcg
     6061 ggatcgggga cgcgtccaag aatccgaaga cgctggaagc cgccgtcgcc gagctggaag
     6121 ccatcaccgg tcagaaggcg atggtgacca aggccaagaa ggcgatcgcg aacttcaacc
     6181 tccgcgccgg cgtgccgatc ggctgcgccg tgacgctgcg gggccccagg atgttcgagt
     6241 tcctggaccg cttcatcaac gtggcggtgc cccggatgcg cgacttccgc gggctgcccg
     6301 acaagagctt cgacgggcgc ggcaactaca cgctcggcat caaggagcag atgattttcc
     6361 ccgagatcga cttcgacaag gtcgagaaga tccacgggat ggacatcacg ttcgtgacca
     6421 ccgcggggcg cgacgacgcg gcgctcgccc tgctgcgcga gatgggcatg ccgttccggg
     6481 gcgaaccccc ggtcgtggcc gcctgaggag attcgaggaa tggcgaagaa gtgctggatc
     6541 gagaaggtca agcggacgcc caagttcaag gtgcgggcgt actaccgctg tcagcgctgc
     6601 ggccggagcc gcgggacgct gagaaagttc aagctctgtc gtatctgttt ccgtgagctc
     6661 gcgctgcggg gagaaatccc gggcgtgcgc aaagccagct ggtaaaggac ggacgaccgc
     6721 atgagcatga ctgatcccgt cgccgatatg ctgacccggg tgcgcaacgc ctgtatggcg
     6781 ggccaccgca gggtcgacat gccggtgtca aaactgaagg ccgagatcgc gcggctgctc
     6841 cgtgacaacg cctacatcca ggacttcaag gtgctggatg acggcaagca cggcctgctg
     6901 cggctctacc tcaagtacca caacgactct cccgtcatcc gcgagctgca gcgggtgtcg
     6961 gcgcccggtc tccgccgcta cgtcaaggcg cgcgagattc cgcggatcaa gaacggcctc
     7021 ggcatggcca tcctctcgac tccgcagggc ctgatgaccg accgcgaagc ccgcaccgcc
     7081 cgcgtgggtg gcgagctcct cgcggtggtc tggtaggagg aacgatgtcg cgcatcggca
     7141 agaaacccgt tcccctgccc aagggcgtca ccgccaccgt ggcgggccac gcgctcaccg
     7201 tgaagggccc caagggagaa atctcccgga ccttccacac ggacatggcg ctggcggtcg
     7261 aggcggagaa cgtggtcgtg aagcggccct ccgacgaggc ccggcacaag gcgctgcatg
     7321 gcctgtcccg gaccctggtc gcgaacatgg tcgagggcgt caccaagggg ttccagaagc
     7381 agctggagat ccagggcgtc ggctacaagg ccgagcccaa gccgttcggc gtgcagctgg
     7441 tcgtggggtt ctcccatccg gtgccgtacc acgcgccgaa gggcatcaag atcacggtcg
     7501 acaacaacgt gaacgtgaag gtcgaaggcg tcgacaagga gctggtgggg caggtcgcgg
     7561 ccgagctgcg cgccgtgaag ccccccgagc cctacaaggg caaaggcatc cgttatcagg
     7621 gcgagcaggt ccggcgcaag gccggcaaga ccgcccaggc ggccaagtaa ccatgcgatc
     7681 gatccggaaa gtcaaatcgg cgaatcacca gcggctccgg cgtcacctgc ggatccgcaa
     7741 ggcgttgacc ggcaccgccg agcgtccgcg tctcgtggtg ttccgttccg ccaagcacat
     7801 ctacgcccag gttgtggacg acaccaaggg cgtgacgctg gtgggcgcgt cggaccgtac
     7861 cgaaggcgtg cagcctgagg gcaagggcaa gacggcgaag agcttcgccc tgggccggca
     7921 catcgcggcc aaggcgaaag aaaagggcat cgcccgcgtg gtgttcgacc gcgggggcta
     7981 tcagtaccac ggtcgcgtca aggcggttgc cgatggcgcg cgcaaaggcg gattggagtt
     8041 ctgatggcga acgaacaaga cccgacggtc ccgacggccg ggacgtcccc gacggccccg
     8101 acggcccccc cagccgctcc ggcggctgaa ggcggcgctc ccgccgaagc taccaccagc
     8161 acgaccggcg gcggcgacgc gcatggccgc ggtggccgtg ggcgcggcgg acgcggtggc
     8221 cggggccggg gtggcgaacg cgggcgcggc ggtgaccgcg gtccccgcgg tgaccgtccc
     8281 cgcgagggcg acggcatggt ggagaacgtg gtcgccatca accgcgtggc caaggtcgtg
     8341 aagggcggcc ggcgcttctc gttcaacgcc ctggtggcgg tgggcgacgg gcaggggcac
     8401 gtcggcgtcg cgaccggcaa ggcgaacgaa gtgtcggaag cggtccgcaa ggcggtggaa
     8461 gccgcccgcc ggaaaatgtc cgagatcccg ctcaccgtgg ggaccattcc ccacgagatc
     8521 gtgggacatc atggcgccgg ccgggtactc ttgaagccgg ccgcgtcggg aactggcgtg
     8581 atcgccggcg gcccggtgcg cgcggtgctg gagtgcgtcg gcatccgcga catcctgacc
     8641 aagagcctgg gatccaccaa cccgcacaac atggtgcgcg cgacgatgga cggcctgaag
     8701 cggctgacca cggcgcggcg tgtggccagg gagcgggggg ttccggatga tgcgattcat
     8761 tacaaggcgc ggcaggaggt gacgcatgtc taggctgccg cccacccgga aataccggaa
     8821 gcggaagccc gggcgtgatc cggttctgcc gccggtaggc aagggcaagc tccgcattga
     8881 gcaggtgcgc tcgacctcgg gccacccttt tcctattcgg agaacgctcg aagccctggg
     8941 cctgaagcat caccaggacg tcgtgatcca gaaggatcat cccgcgttgc ggggtatgct
     9001 gtatcacgtg cgtcacctca tcaaggtgac gccggcgaaa gacggagcgt aagcgatggc
     9061 gaagacgacg accaagaagc cccgcgcccg gaaggccaag gcggaggcgc ctcaggtgga
     9121 gccgctgcgg ctgcatcacc tgaagcccgc ccccggctcg acccaggcac ggcggcgcgt
     9181 cggccgcggc cccggctcgg ggttggggaa gacgtccggc aagggcaaca agggtgagaa
     9241 ggcgcgctcg ggcggcggcc gccgtccggg attcgaaggg ggccagatgc ccctgattcg
     9301 gcgcattccc aagcgcgggt tcaccaaccc gttcaagcag ccggcccagg tggtgaacgt
     9361 ccggcacctc tccttcctca aggatgggct cgaggtgact cccgagacgc tcttcggcgc
     9421 cggcctggtg cggcgtcccg atcaccccat caagctcctc ggcatgggtg acgtggcccg
     9481 caagttcacc gtgaagggtg tttcggtgtc gacctccgcc aagggcaaga tcgaggcggc
     9541 gggcggcatc atcgagggct gattccacgt gactgcgccc gcaattccga atctgttcaa
     9601 ggttccggag ctcaagaaca agatcctctt cacgctgctc tgcctggtga tctaccgggt
     9661 gggcgcgcat atcgcgacgc ccggcgtgaa cgtccaggct ctttccgacg cgctgcagca
     9721 ggcggggaag ggcaacctct tcgggctcta cgatctcttc gccggcggcg ggctgggacg
     9781 ggcgaccgtc ttcgcgctcg gcatcatgcc gtacatctcc gcgagcatcg tcttccagat
     9841 ggcgggaccg gtgttcccca tcctcgagaa gatgcagcgg gacgaagagg gccggaaaaa
     9901 gatcacgcag tggacccggt acctcacggt cgtgctctgc ttcctgcagg cctacggtta
     9961 cgcgctcttc gtcgagacgc aggtgcccgg ggcggtgctc aatcccggca tggggttccg
    10021 tctcacgacg gtcctcacgc ttaccaccgg cggcatcttc gtcatgtggc tgggtgagca
    10081 gatcacggag cgcggcatcg gcaacggcgc gtcgctcctg atcttcttct cgatcatcga
    10141 gcgcatctgg cccgagttca tcaatacgtt cgattcgatc aaggcgggga ccgcgagcgt
    10201 gaagctcatc ctcttcgtga tcgtgatgat ggtgatgatc accggggcca cggtcgcgat
    10261 caccgtcgcg gcgcggaaga ttccgatcca gatcccgcgc aaggtgatgg gacgcggacg
    10321 catccgggaa ggccagaaga cgttcatccc gctccgcctg ctcacggcga acgtgatgcc
    10381 catcatcttc gcccagtccc tgatcatcat tccgagcacg ctcgcgacct tcgtgggcgg
    10441 ctcgctcctg cggcaggtca acgactactt cagcgccggc tcggcactct atatcgtgtc
    10501 cctgctggtc ctgattctct tcttcaccta cttctacacg tccatcgtct tcaatccggt
    10561 ggatctggcg gagaatctga agaagcaggg gggattcatc cccggcgtga agcccggcgc
    10621 ggcgacggcg gactacatcg acgaagtgct ctcccggatc accttgccgg gcgggttctt
    10681 cctcgcgctg tgcgccatca tcccgatcat cgcggcgaat ggcctgaacc tgcccttcaa
    10741 cttcggcggg acggcgctga tcatcgtggt gggcgtggcg ctggacacga tccagcaggt
    10801 gcagcagcac atgctcctca ggcactacga cgggttcatg aaggaaggcc gcgtgaagtt
    10861 ccgcgggcgg acccgctagc ccacctacac aaggcccaca acgtgttcat cctcctcctc
    10921 ggtgcaccgg gatcaggaaa gggcacccag ggcaagctcc tcagtgagcg ccttgggctc
    10981 ccgaagatca ctaccgggga cctgatccgg accgccatca agacgggcac tcccctcggc
    11041 gtcgaagcca agaagttcta cgacgccggc aagctcgtgc ccgactccgt catcatgggg
    11101 ttgatccagg agcaattggc gaagccggag gccaaggagg gcgcgattct ggacggcttc
    11161 ccgcgcaccg cggcgcaggc ggagctggtg gaccgcacct tggcgacgac ccagcaccgg
    11221 ttgaaccacg tcctgcttct cgacgttccg gaggaggagt tggtgagccg gatgttggga
    11281 cgggcgcggc aggaagggcg cgcggacgac acgccggagg ccatcaagac ccggctcggc
    11341 gtctatcagg tcgagacggc gcccttggtg gcgcacttcg cccagcgggg catcgtgcac
    11401 cgggtgccgg ggaccggcac cgtcgatcag atcgccgagg agatcaagcg aatcatcgga
    11461 cgatgatcac catcaagtcg ggccgcgaga ttgaaacgat ggccgaggcg gggaagatcg
    11521 tggcgaagac cctcgcgctg gtggcggcca acgtgcgtcc cggtgtctcc acgcagcagc
    11581 tcgatgagct ggccgaagag ttcatccgca gtcatcccgg tgccaagccg tctttcaagg
    11641 gtttgtacga ctatcctgcc accctctgca cgtcgatcaa tcaggaggtc gtgcatggga
    11701 ttccctcgtc caagcgcgtc ctcaaggagg gggacctcgt cagcgtggat gtcggcgcct
    11761 ggctggacgg ccttcatgcc gactcggcgg ccacctttcc ggttggggtg attcccgagg
    11821 ccgcgcagcg gctcttggat gtcacgaaga aagcgttggc ggcggggatt gccgaggcgc
    11881 gcgcggggaa tcataccggt gacatcggcg cggcggtcca gcaggtggcc gagggggccg
    11941 ggtattcggt ggtgcgggag ctggtgggtc acggcatcgg ctcggcattt catgaggagc
    12001 cgcaggtccc gaactatggg aagcccaagc ggggtccgcg gctggtggcg ggcatgacga
    12061 tcgcgatcga gcccatggtg aatgccggcg gggccgacat ccggacgctt ccggacaagt
    12121 ggacggtggt gacggaggac gggtcgttgt cggcgcattt cgagcatacg gtggcggtgg
    12181 gtcccgaagg cactcctccc aggattctga cgaccgcagc gtagtctccc tcggattggc
    12241 agtgtgcggc ttgggcggta tgttcccgac atgaagccgc gatggagcaa gaggctcttg
    12301 gttgcatgcg cgctgggagt cggattccca cccctgtcag ctcagtcctc attcaagcaa
    12361 gtcagggtcg aatcctgcgc ggtcgccgat agcgtcctcg gtccagtcgc cggtgatcag
    12421 gatgcggtgg ttcggctgca ctacttcgcg agtgtcgaca ccacgcgcct cgttgcgggt
    12481 ctcgagcgaa ctcactccat cgatcccgat ggctccgacg tcttgttcat gacctcctat
    12541 gggggtcatc gcccgactgt ctatccccat cttgatctct tctttgtgtt gaggggtgcc
    12601 ccgaccctgg tgggaacgcc gggtgactcc tcacccgtgg tgttgctggt tgacggatcc
    12661 gcaattccca ttgggtcggt aatcgtacca gcgggctcgg cgttctcatt ccgcatgacg
    12721 ctgacccgga atcaagcctt cctgctggcg ggagccacga ctgcgtcctt caccatttac
    12781 cacttgagcc acgccgtgac gcgcgaggaa ctccgcgata ttcgcgggat gtaccgggtc
    12841 gcactctgcg gccagtccac tgccgaggcg gagggtgact cgacaccccg cgtggccaac
    12901 ttggccggtt actggcgtct gagcggaccg acggtgcccg gcaggctggt cgccacgttc
    12961 acgcaggatg gtaaccagct gcgggtcaac attgtcgcga aggatcagtg tggacgtgac
    13021 cttctgacgg ttgacttgac gcttcttggg gaagtggacg ccgacaccgt gcgattctgg
    13081 tccggctccc ggagtttcga ggggtatttg ggcaacgcgt gcgcgctcta tgccgagttc
    13141 caggagaagg tgaagttcga aggggtgctg acggcggatg gcaagagttt tgaggggagg
    13201 tacgatgaca ccggcctccc gacgcacgtg tggaggttct cccggtagtc tggcgcgccc
    13261 tgaaggcctg agcccgccgc gaatcggcgg gctttcgcgt ttttccggta cgctgttcct
    13321 gctcctcagc gcgtatggtc cagcgtgccg gcttcttcgt ctctgctcac ccaactccgt
    13381 gaagccctcc ggacgcggca ctacagtccg cggacggagg aggcgtacgt ccagtgggt
//
LOCUS       DASUBP010000076         5578 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_274228, whole genome shotgun sequence.
ACCESSION   DASUBP010000076 DASUBP010000000
VERSION     DASUBP010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5578)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5578
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_274228"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1773
                     /gene="alaS"
                     /locus_tag="VFU75_03080"
     CDS             <1..1773
                     /gene="alaS"
                     /locus_tag="VFU75_03080"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEU5465843.1"
                     /translation="APYRVLADHARAVTFLLADGVAPSSDGRGYVLRRILRRGVRYAW
                     QEGRAEPTLALLAERVIETMGDVYPDLIAKKAEIVRATKAEEERFLETIEGGMRRLDE
                     LESAKTISGEDAFKLYDTYGFPLDLTQVIATERHQAVDVEGFEAALAQQRRRSRAARV
                     SATAAIAQAGKRGEWVDVRPEGRQRFIGYDETASETDVLAFRQTGELVELVLQDNPFY
                     AESGGQVSDTGVVTGDGWELTVEDVVKENGKSAVVGRFGETFEPTAVRARVNEARRRN
                     IERNHTGTHLVHAALRKILGTHVRQRGSLVAPERLRFDFSHHGPIDDRVLANIEEEVN
                     GRILENLPVTTQEMARDEAIAKGAMALFGEKYGERVRVVDIGGGVSLELCGGTHVQAT
                     GQIALFRFTHETGAEAGVRRIEAITGPSAYRHLREIEERLASAARLLKAQPEHIEKRI
                     ETLLEERHKLERQIQEMLKGAGSRESGVVSRVGDVELHVADSPLEDREQIALVMDAFR
                     QGRSRAISVLFTRGERPGIHVAVTDDLVEKGVHAGKLAGQIAAVSGGKGGGRPHFASA
                     GAGDAARLDSAREQTPVIVKGMLG"
     gene            1770..2072
                     /locus_tag="VFU75_03085"
     CDS             1770..2072
                     /locus_tag="VFU75_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465844.1"
                     /translation="MSAEDLSTWLSGRKPAAPEALAGRIADAVGNEAGAPPRTLAMLG
                     ARLLDRVAAATTQDRRQALDLLAADALVTYAFEAQAEADVRGLTTLAERISKEGQA"
     gene            2069..2821
                     /locus_tag="VFU75_03090"
     CDS             2069..2821
                     /locus_tag="VFU75_03090"
                     /inference="COORDINATES: protein motif:HMM:NF014000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglyceryl/heptaprenylglyceryl
                     phosphate synthase"
                     /protein_id="HEU5465845.1"
                     /translation="MKNLLSRRPGVLILVDPSRTPAAEATRVARAAHQAGAAGVMIGS
                     SYDGTRETHQVARALRDGASELPVALFPGSAMQLTEDVDLVLFLSLISGRNAQYLIEE
                     HVRAVPFFQRHQVPALSTGYILIDGGRVTSVEAVSQTRPLPADKPELAAAHALAARMI
                     GMSAVYLDAGSGAAHPVPPAIVAACRAALSPDVTLFVGGGIRTPAEAAAARKAGADFV
                     VIGTAVEQGSDESWRTQIESFVRAVGASGVRE"
     gene            2818..3393
                     /locus_tag="VFU75_03095"
     CDS             2818..3393
                     /locus_tag="VFU75_03095"
                     /inference="COORDINATES: protein motif:HMM:NF024969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEU5465846.1"
                     /translation="MNPTERIRAALTSLSALADRVASEQAGAVADLAARYEAVLRGGG
                     TLFFAGNGGSAADAQHIAAEYVVRYQTTRPGLSAIALTTDTSVLTACANDLGFDEVFA
                     RQVESHVRPGDLLVLHSTSGESPNVIRAAQSAKARGVPVVAFLGKSGGPLKALADVAL
                     VVPADETARIQELHLAIEHIICDIVEERMKG"
     gene            3457..4209
                     /locus_tag="VFU75_03100"
     CDS             3457..4209
                     /locus_tag="VFU75_03100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5465847.1"
                     /translation="MLGIQGKRAFVTGGTRGIGLATAELLVRSGARVALGYRSRRADA
                     DAAVAKLGSAAVAVAGDLGVPAAARTAVDDAVRQLGGLDILVVNHGVWPPQEVPLSQM
                     TDAQWESTRQANYDAAFYVCRAAIPHLPNGGKIVLVSSTAGQRGEAFHADYGAARGGV
                     IAFTKSLAVELAPGINVNCVAPGWVDTEMAKVPYEREGGKGKREIEASIPLKRVASAA
                     DVAGPIVFLCSDLARHITGEILNVNGGSVLCG"
     gene            4286..5494
                     /locus_tag="VFU75_03105"
     CDS             4286..5494
                     /locus_tag="VFU75_03105"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465848.1"
                     /translation="MTFPTTIAVPDNVLRIATKLEEAGFETWCVGGAVRDTLLGLPHH
                     DFDLTTAAHPEKVRELFRRTVPVGIEHGTVAVLDGKGQPHEVTTFRRDIKTDGRHAVV
                     EFGGSLMDDLARRDFTINAIAYHPLRHEWKDPFDGRADLDAHVIRAVGDPNWRFQEDY
                     LRILRALRFSARFEFRIHSRTLEAAKANAQGLAQLSAERVRDEWWKGIATAKRVSKLL
                     SLWRDVGAARIWLPEIQNTGNEKRETGNVDKLPRDPVLVSSVLASDPPSLLTRLKCSN
                     RDIERARAIKTWRGEYPDPRHLPQVRRWLAKTGAYANDLLAILPGINVSRFPFPVSRL
                     PLIVESVRAAQDPLSVSDLAVNGADLLAAGVRPGPEIGETLARLLDAVLEDPARNTRD
                     ELLQRVGRHG"
BASE COUNT          875 a         1838 c         2041 g          824 t
ORIGIN      
        1 gcgccctatc gcgtgctcgc cgatcacgcg cgcgcggtca cgttcctcct ggcggatggg
       61 gtcgcgccat cgtccgacgg ccgcggctac gtgctgcgcc gcattctgcg ccgtggcgtc
      121 cggtacgcgt ggcaggaagg ccgtgccgag ccgaccctgg ccctcctggc ggagcgggtg
      181 atcgagacga tgggggacgt gtaccccgat ctgatcgcga aaaaggcgga aatcgtccgc
      241 gccacgaagg cggaggagga gcgtttcctc gagaccatcg aaggcgggat gcggcgcctg
      301 gacgagctcg agtccgccaa gaccatttcg ggtgaggacg cgttcaagct gtacgacacc
      361 tacggctttc cgctcgatct cacgcaggtc atcgcgaccg agcgtcacca ggcggtggac
      421 gtcgaggggt tcgaagccgc tctggcgcag cagcgccggc gttcgcgcgc ggcgcgcgtc
      481 tccgccaccg cggccatcgc gcaggcgggg aagcggggcg agtgggtgga cgtgcgcccc
      541 gagggccgcc agcgcttcat cggctacgat gaaacggcct ccgaaaccga cgtgctcgcc
      601 ttccggcaga ccggtgagct ggtggagctc gtgctgcagg acaatccgtt ctatgccgag
      661 tcgggcggcc aggtgtccga cacgggggtc gtgaccggcg atgggtggga gctgacggtc
      721 gaagacgtcg tgaaggagaa cgggaagagc gccgtggtgg gaaggttcgg cgaaacgttc
      781 gagcccaccg cggtccgcgc ccgcgtcaac gaagcccggc gccggaacat cgagcggaat
      841 cacaccggga cccacctggt gcacgccgcc ctgcggaaga tcctggggac gcacgtgcgc
      901 cagcgcggct cgctggtggc gccggagcgc ctgcggttcg acttcagcca ccacggcccc
      961 atcgacgacc gggtcctcgc caacatcgag gaagaggtga acggcaggat tctggagaat
     1021 ctgccggtca cgacgcagga gatggcgcgg gacgaggcga tcgcgaaggg agcgatggcg
     1081 ctcttcggcg agaagtacgg cgagcgggtg cgggtggtgg acatcggcgg cggcgtgtcc
     1141 ctcgagctgt gcgggggcac gcacgtgcag gcgaccggcc agatcgcgct gttccgcttc
     1201 acgcacgaga ccggcgcgga agcgggggtg cgccgcatcg aagcgatcac cggcccttcc
     1261 gcgtaccgcc atctgcggga aatcgaggaa cggctggcgt ccgcggcgcg gctgctcaag
     1321 gcccagcccg agcacatcga gaagcgcatc gagacgttgc tcgaggagcg tcacaagctc
     1381 gagcggcaga tccaggagat gctgaagggc gccgggagcc gggagtcggg agtcgtcagc
     1441 cgcgtgggcg atgtggagct ccacgttgcg gactcccccc tcgaagatcg cgagcaaatc
     1501 gctctcgtca tggacgcctt ccgccaggga cgttcgcggg cgatatctgt gctcttcaca
     1561 cggggcgaac gcccggggat ccacgtggcc gtgacggacg atctggtgga aaagggcgta
     1621 catgcgggca agctcgccgg gcagatcgcc gccgtgagtg gcggaaaggg cgggggacgt
     1681 ccgcacttcg cctccgcggg cgcgggggat gcggcgcgcc tggattccgc gcgcgagcag
     1741 acgccggtga tcgtgaaggg aatgctcggg tgagcgccga agacctgtcg acctggctgt
     1801 cgggacggaa gcccgcggcg ccggaggctc tggcgggacg gattgcggac gccgtgggca
     1861 atgaagccgg cgcgcccccg cggacgttgg ccatgctggg cgccaggctg ctggatcggg
     1921 tcgcggcggc gaccacccag gaccgccgcc aggcactcga tctgctggcc gccgacgccc
     1981 tcgtgaccta cgccttcgaa gcgcaggcgg aagcggacgt gcgtggactg accaccctgg
     2041 cggagcggat cagcaaggag gggcaggcgt gaagaatctg ttgtcgcgcc gtcccggcgt
     2101 cctgatcctg gtcgacccca gccgcacgcc cgcggcggag gccacgcgcg tggcccgcgc
     2161 ggcgcaccag gcgggcgccg ccggtgtcat gatcgggtcc tcctatgacg gaacccgcga
     2221 gacccatcag gtggcacgcg ccctgcgcga tggcgcgtcc gagctccccg tcgcgctctt
     2281 ccccggatcg gccatgcagc tcaccgagga tgtcgatctg gtgctgtttc tctcgctgat
     2341 ctccgggcgc aacgcgcagt acctcatcga agagcacgtg cgggcggtgc cgttcttcca
     2401 gcggcatcag gtgcccgcgc tctcgacggg atacatcctg atcgacggcg ggcgcgtcac
     2461 cagcgtggaa gcggtgagcc agacccgtcc cctcccggcc gacaagcccg agctcgcggc
     2521 cgcgcacgcg ctcgccgccc gcatgatcgg catgagcgcg gtgtatctcg acgcgggaag
     2581 cggtgcggca catcccgtgc cccccgcgat cgtggccgcg tgccgcgccg ccctcagccc
     2641 cgacgtgacg ctcttcgtgg gtggcggcat cagaaccccg gccgaggcgg cggcggcccg
     2701 gaaggccggc gcggacttcg tcgtgatcgg caccgcggtc gaacagggga gcgacgagag
     2761 ctggcgtacc cagatcgagt cgttcgtgcg ggccgtgggg gcgtccgggg tgcgcgagtg
     2821 aatcccaccg agcggatccg cgcggcgctc acgagcctgt cggcgctggc cgaccgcgtg
     2881 gccagcgaac aggcgggggc cgtcgcggac ctggccgccc ggtatgaagc ggtattgcgc
     2941 gggggtggga cgctgttctt cgcggggaat gggggcagcg cggcggacgc gcagcacatc
     3001 gccgccgagt acgtcgtgcg ttatcagacg acgaggccgg gcttgtcggc gatcgcgctg
     3061 accacggata cgtcggtgct caccgcgtgc gcgaacgatc tcggattcga cgaggtgttc
     3121 gcccggcagg tcgagtcgca cgtccggccc ggggatctgc tggtgctgca ttccacgtcg
     3181 ggagagagcc cgaacgtgat tcgcgcggcc cagtccgcga aggcgcgcgg tgttcctgtc
     3241 gtcgcttttc tcggcaagtc gggcgggccg ctcaaggcgc tcgcggacgt ggcgctggtc
     3301 gtccctgcgg atgagacggc gcggatccag gagctccacc tcgccatcga gcacatcatc
     3361 tgtgatatcg tggaagagag gatgaaaggc tgagaaggct agtcgccaag tcgctaagtc
     3421 gccaagtcgc tgagtagagg ccaggctggg agggtgatgc tcgggattca gggcaagcgc
     3481 gcattcgtga ccgggggaac ccgggggatc gggctcgcga ccgctgaact gctggtgcgg
     3541 tcgggagccc gcgtcgcgct cgggtaccgg tcccgccggg ccgacgcgga cgccgcggtc
     3601 gcgaagctcg gttccgcggc ggtggcggtc gccggggacc tgggcgtgcc ggccgccgcc
     3661 cggaccgccg tggatgacgc ggtccgccag ctcggcggcc tggacatcct cgtggtgaac
     3721 cacggcgtgt ggccgcctca ggaagtcccc ctgtcccaga tgaccgacgc gcagtgggag
     3781 tcgacgcgtc aggccaacta cgacgcggcg ttctacgtct gccgcgcggc cattccgcat
     3841 ctccccaatg gcgggaagat cgtgctcgtg tcgtcgacgg cgggtcagcg gggggaagcg
     3901 ttccatgccg actacggggc ggcccggggc ggggtcatcg cgttcaccaa gtcgttggcg
     3961 gtggagctcg ctcccggcat caacgtgaac tgcgtggccc cgggctgggt ggacacggag
     4021 atggcgaagg tgccctatga gcgggagggg ggaaagggga agcgggagat cgaggcctcc
     4081 attcccttga agcgcgtcgc ctcggccgcc gacgtcgctg gacccatcgt cttcctgtgt
     4141 tccgatctgg cccgccatat caccggcgag atcctcaacg tcaacggggg atcggtgctg
     4201 tgcggatgat gagaacgcgg aggtcggaac tcggaatgcg gaaccggtgt tccgcgttcc
     4261 acgctccgcg ttccgagttc ctggcatgac cttcccgacc acaatcgctg ttcccgacaa
     4321 cgttctccgg atcgcgacca aactcgaaga ggccggcttc gaaacctggt gcgtgggggg
     4381 cgcggtgcgg gacaccctcc tgggcctgcc gcatcacgac ttcgacctca ccacggcggc
     4441 gcacccggag aaagtgcggg agctgttccg gcggacggtg ccggtaggca tcgagcatgg
     4501 cacggtcgcc gtactcgacg gcaagggtca gccgcacgag gtcacgacgt tccgccggga
     4561 catcaagacc gacgggcgac acgccgtcgt ggagttcggc gggtcactca tggacgatct
     4621 cgcccgccgg gacttcacca tcaacgccat cgcctaccat ccgctcaggc acgagtggaa
     4681 agaccccttt gacggccgcg cggacctcga cgcccatgtg attcgcgccg tgggggatcc
     4741 caactggcgt ttccaggagg actatctccg catcttgcgg gccctgcgct tcagcgcccg
     4801 gttcgagttc cggattcatt cccggacgct cgaggctgcg aaggccaacg cgcagggact
     4861 cgcgcagctc tcggcggagc gggtacgcga cgagtggtgg aagggcatcg cgacggcgaa
     4921 gcgggtcagc aagctcctct cgctctggcg cgatgtcggg gccgcgagga tctggctgcc
     4981 ggaaatacaa aacacgggaa acgagaaacg ggagacggga aacgtagaca aacttccccg
     5041 tgatccggtg ctcgtgtcca gcgtgctggc atcggatccg ccctcgctct tgacccggct
     5101 caagtgctcc aaccgggaca tcgagcgcgc gcgggcgatc aagacctggc gcggtgagta
     5161 cccggatcca cgtcacctcc cccaggtgcg gcgctggctg gcgaagaccg gggcatacgc
     5221 caacgacctg ctggcgatac tccccggtat caacgtgtcc cgcttcccgt ttcccgtttc
     5281 tcgactcccc ctgatcgtgg agtcggtgcg ggcggcgcaa gatccccttt ccgtgtccga
     5341 tctggccgtg aacggggccg acctgctcgc cgccggcgtc cgccccgggc ccgaaatcgg
     5401 agagaccctg gcccgcctgc tcgacgccgt gctggaagac cccgcccgca acacgcgcga
     5461 cgagctgctg cagcgggtag gccgccatgg gtgaggccct cggctacgcc gtcgtcgcgg
     5521 cgctcggcaa cgtgctcggt gccctcgcgg tcacccggca tgccgcccgg agcctcac
//
LOCUS       DASUBP010000077         7542 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_683069, whole genome shotgun sequence.
ACCESSION   DASUBP010000077 DASUBP010000000
VERSION     DASUBP010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7542)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7542
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683069"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..470)
                     /locus_tag="VFU75_03110"
     CDS             complement(<1..470)
                     /locus_tag="VFU75_03110"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5465849.1"
                     /translation="MEANRDAARLARNGLIVLSLINLFNYLDRYVVPALFESLKQSPL
                     HPTDGQLGFLMSGFLIVYTVTAPIFGRSGDRGRRPRVLAFGVFLWSLATAAGGLARSY
                     LQLLLARAAVGVGEAAYGTVGPSLLADYFPRSSRGRAFAVFFIALPVGSALGFVV"
     gene            519..773
                     /locus_tag="VFU75_03115"
     CDS             519..773
                     /locus_tag="VFU75_03115"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU5465850.1"
                     /translation="MTHGWTVYLLRCRDGSLYTGITNDLPRRLASHRAGTGSAYVRTR
                     LPIRVVYRESQPDRGAALRREAAIKKLPRRDKLALVRRRG"
     gene            770..1417
                     /locus_tag="VFU75_03120"
     CDS             770..1417
                     /locus_tag="VFU75_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HEU5465851.1"
                     /translation="MTRGKDVAGLAVSILIALVCVRLGIWQIARLHQRRAANAEIALR
                     ERGPVLEVTGAESLDSVQWRRVRASGVYDYAREKIWTGRTFQGVPGVALLTPLRIAKG
                     AEIWVDRGWVPSPDAAQVDQGRWREGDSATVAGLAMAHEGLPWVIDDSIPAKDRAPDP
                     TIQRWPVPTLTAGPHLSYVIQWFSFAAIILGGSLTLFWKRGLALQDGRSHSPTTI"
     gene            1514..2194
                     /locus_tag="VFU75_03125"
     CDS             1514..2194
                     /locus_tag="VFU75_03125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5465852.1"
                     /translation="MPAATPIKRPIKVSQLVRRRLKELKRTPRELAEAIQVSEEYVGD
                     LLAGRRAPPAPGRTDLYDRMTKFLRLHRNDLPTCAKVERESMDTKQKRPDPKVRALLL
                     ELCEPVKAKKLDRRFTKRDQETAALDRLVAQRLLEVAQGFVRRQLDDEVGIRVAANRE
                     GVTYLQMRMRLLEFLDATVESLSVTDYDVFIRPRIGFWEIDLETNAMRIVLRSQEPAP
                     RQRRAMGF"
     gene            complement(2276..2485)
                     /locus_tag="VFU75_03130"
     CDS             complement(2276..2485)
                     /locus_tag="VFU75_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004094893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEU5465853.1"
                     /translation="MARITGTVKWFNDAKGFGFITPENGEKDCFVHHTAIQADGFRSL
                     AEGQRVEFEVVQGAKGPAAQNVIKL"
     gene            2817..6182
                     /locus_tag="VFU75_03135"
     CDS             2817..6182
                     /locus_tag="VFU75_03135"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5465854.1"
                     /translation="MYRILAVTLAALVLPVRPAFLPASATVPEGQAVQAVRAAADTGK
                     KKSDLPLTTTRTVSFSTSEGTWLSLDVSPDGKTIVFELLGDLYTMPITGGTATRITSG
                     PAFDSQPRWSKDGKEIVFLSDRSGAENIWLCDADGSHAKAVTKGSNNLYASPVFTPDG
                     NYIVASRTSGVLGSDYELWLYHKDGGSGTAMVKAGTPAPGVPQVNAMGAAFGNDPRYV
                     YFARKRGGFGYNLQIDGGQEWQIAVYDRQTGQTNNVTDVNGSGMRPIVSPDGKWLVYA
                     NRHDTATGLRLRDLATGDEKWLVYPVTRDDQESRFTRDLEPNSAFTPDSKALVTNYGG
                     KIWRVEVPSGQVSAIPFTAQVEQQLGPLVRFDFRVDTGEITLHQIRDASPSPDGKRLV
                     FSALDKLYVMDLPNGTPRRLTSAAVHEQEPVWSPDGKWVAYITWTDTSGTVSKVSASG
                     GNPTVLTSEPGFYEQPAWSPDGNRLVFYKGPRQAHETEHRGAGEDLVWMPAAGGATTR
                     ITRMEGGGRPHFARDPNRVFVYDGNELVSMRFDGTDRRTWIKVTGYTYPGPGSEPSTA
                     DEIIISPDSDRVLAAAGNNVYLVTLPEVGGTPPSINISDPSSASFPVKRLTTIGGDFI
                     GWAPDGKRVFWSIGRSFFRYEPAVADSLEKAKAVQDSLRTESLKGDTLANGKPDTVAK
                     ARVDSLNKKPAYEADRSDVTIKVARDVPKGTVVLKNARIITMKGDEVIEKGDLVVTGN
                     RIACVGTCTTPAGATVMDLAGKTIIPGLIDTHAHPWPEWGIHSSQVWKYLANLAFGVT
                     TTRDPQTATTDVLTYADMVEAGQIIGPRIYHTGPGVFWSEDIKSLDDAKNILKRYSEF
                     YGVHTIKEYMTGNRKQRQWVIMAAKELHLMPTTEGGLDFKMNLTEALDGYPGHEHSYP
                     IMPLYNDAVQLIAQSGINYNPTLLVNYGGPFAENYFYEHYNIHDDPKIRRFFPHDQID
                     QRALRRPGWFADDQYVYQQIAASAAKIVRAGGHVGIGCHGQFDGEGCHWETWAFVAGG
                     MTPLEALRVATHDGAYAIGMEQDLGSLEPGKLADLVVLDANPLEDIHNTTKISTVMKN
                     GRLYDGNTLDQVWPDKVTLPRMWWWNQEPGGKN"
     gene            6461..>7542
                     /locus_tag="VFU75_03140"
     CDS             6461..>7542
                     /locus_tag="VFU75_03140"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5465855.1"
                     /translation="MPFAALGLVPELVRAVAEEGYEQPTPIQQETIPLALEGRDLIGS
                     AQTGTGKTAAFMLPILQRLAKHGTGTRALVLVPTRELAEQVATSARAYGRHLPLAAVA
                     VYGGVGMEPQTKALTRGVDIVVATPGRLLDHMERGHVNFSKLEILVLDEADRMLDMGF
                     APDVNRILRALPGERQTMLFSATVSREVDALARKALNGHASVEIGRRAQTAEGIEHVI
                     VAVDKAQKKDVLVKILQAKPEGQTLVFTRTKYGADKLVTHLRKNGIGAHALHGDKAQS
                     HRQRTLDEFRDGRTEILVATDIAARGIDVDGIRMVVNFDVPNDPEAYVHRVGRTARAG
                     AQGLGLTLISPDEWLMMADVEKLIGKT"
BASE COUNT         1374 a         2478 c         2502 g         1188 t
ORIGIN      
        1 acgacgaagc ccagggccga cccgaccggg agggcgatga aaaagacggc gaacgcacga
       61 cctcggctgg agcgggggaa gtagtccgcc aggagactcg ggccgacggt tccgtaggcg
      121 gcttctccca cgcccaccgc cgcgcgagcg aggagcagct gcaggtaact ccgggcgagt
      181 ccgccggccg cggtggccaa actccagagg aacacgccga aggcgagtac ccggggtcgt
      241 ctcccgcggt cgccggaccg gccgaagatc ggggcggtga ccgtgtacac gatcaggaat
      301 ccgctcatca ggaagcccag ctgcccatcg gtggggtgga gcggcgattg cttcagactt
      361 tcgaacagcg ccggtaccac ataccggtcg agatagttga agagattgat gagcgagagg
      421 acgatcagcc cattgcgcgc gagtcgcgcg gcgtcgcggt tggcttccac ggcggctgaa
      481 aatggcggcg gccggggggc cccgctagtt tccgcgccat gacgcatggc tggaccgtct
      541 acctgttgcg ctgccgtgac ggctcgctct acaccggcat caccaacgac ctcccccggc
      601 ggctcgcgag ccatcgcgcg ggcaccggaa gcgcctacgt gcgcacgcgt ctgcccattc
      661 gcgtcgtcta tcgggagtcc cagcccgacc ggggggcggc gctccgccgc gaggcggcga
      721 tcaagaaact cccgcggcga gacaagctcg cgctggtccg gaggcgggga tgacccgggg
      781 gaaagacgtc gcgggactgg cggtttcgat cctcatcgcg ctcgtgtgcg tacgcctcgg
      841 catctggcaa atcgcccggc tccatcagcg gcgtgccgcc aatgcggaga tcgcgctccg
      901 ggagcggggg cccgtcctcg aagtgaccgg agcggaatcc ctcgattccg tgcaatggcg
      961 gcgggtacgg gcgagcggcg tgtacgacta cgcgcgggaa aaaatctgga ccggccggac
     1021 gtttcagggt gtgccggggg tcgctctgtt gacccccctt cggatcgcca aaggtgccga
     1081 gatctgggtg gatcgcggtt gggtcccgtc tcccgatgcc gcgcaggtgg accagggccg
     1141 ctggcgggag ggagacagcg ccaccgtggc gggactcgcg atggcgcatg aaggccttcc
     1201 ctgggtcatc gacgactcga tccccgcaaa ggaccgggcc cccgacccga ccattcagcg
     1261 gtggcccgtc ccgacgctga cggccggtcc tcacctttcc tacgtcattc agtggttctc
     1321 cttcgccgcc atcattctcg ggggatccct gactctcttc tggaaacggg gattggcgtt
     1381 acaggacggg cgaagtcact cacccactac catttagagg tccgtctggt gatattgtgt
     1441 ccggcagcgg cccgcccaca cgcgcccgta agctgatgcg tggagcgggc tttcgcgtac
     1501 cgggggtaac tgaatgcctg cagcgacacc gatcaaacgt cccatcaagg tctctcagct
     1561 ggtccgccgt cgtctcaagg aactcaaacg cactccccgc gagctggccg aagccatcca
     1621 ggtctccgag gaatacgtgg gtgatcttct ggcggggcgc cgcgcgcctc cggcgccggg
     1681 ccgaaccgat ctgtatgacc gcatgaccaa gtttctgcgc ctgcaccgca acgatctgcc
     1741 gacgtgcgcc aaggtcgagc gggaatcgat ggatacgaag cagaagcgcc ccgaccccaa
     1801 ggtccgggcg ctgctgctgg agttgtgcga gccggtcaag gccaagaagc tcgaccgccg
     1861 ctttacgaag cgggatcagg agacggccgc actcgatcgc ctcgtggccc agcggctgct
     1921 ggaggtggcg cagggtttcg tgcgccggca gctggatgac gaagtcggca tccgcgtcgc
     1981 cgccaaccgc gagggtgtca cgtatctgca gatgcggatg cgtctcctcg agttcctcga
     2041 cgcgaccgtc gagtcactct cggtgaccga ttacgacgtg ttcatcaggc cgcggatcgg
     2101 gttctgggaa atcgatctgg aaacgaacgc gatgcggatc gtcctgcggt cgcaggaacc
     2161 cgcgccgcgt cagcgccggg cgatggggtt ctaggcaact cgagcagctc gtaaaacacg
     2221 gcgggccacc tcttgcgaga tggcccgccg ttttcatcgt ggtaccgcgc gttcgttaaa
     2281 gcttgatgac gttctgcgcg gccgggccct tcgcgccctg gacgacttcg aactcaaccc
     2341 gctgaccctc ggcgagggaa cggaaaccat ccgcctggat agcggtgtga tgcacgaagc
     2401 agtccttttc gccgttctcg ggcgtgatga agccgaagcc cttcgcgtcg ttgaaccact
     2461 tcacggtacc ggtgatacgt gccatactgg tgtgactcct gatgtgttat gacccggtcg
     2521 gctagagccg ccgtcgagcg tcggaagtga ctcccagata cacgaacagg accataggcg
     2581 atgcccaggt cctgtgacgg tctggttctt tcgaccccgg caccatgccg gagccccttc
     2641 gctcgacacg aagaatcata tgatgtcacg attctgtcaa gcccccgcgg gccatccctg
     2701 aggcgctaaa gccaaggagg cccacggttt tgctcgacgt ctttgcttga cgctcgttct
     2761 tcccgccttc agtttcttgc ccgccacgcc gtcagcttct caccaacgga gccccgatgt
     2821 accgaatcct ggccgtgacc ctggccgccc tcgtgctgcc cgtacgcccc gcttttctcc
     2881 ctgcttcggc gaccgtccct gaagggcagg ccgtccaggc cgtccgggct gccgccgaca
     2941 ccggaaagaa gaagagcgac ctccccctca ctaccacccg gaccgtctcg ttctcgacgt
     3001 ccgaagggac ctggctctcg ctcgacgtct cgcccgacgg caagacgatc gtgttcgagc
     3061 tgctgggaga cctgtacacg atgccgatca cgggcggcac ggcgacccgg atcacgtcgg
     3121 gacccgcgtt cgattcccag ccgcggtggt cgaaagacgg caaggagatc gtcttcctgt
     3181 cggaccggag cggagcggaa aatatctggc tctgtgacgc cgacggcagc catgcgaaag
     3241 cggtcaccaa gggcagcaac aacctgtacg cctccccggt gttcactccc gacggcaact
     3301 acatcgtcgc ctcccgcacc tcgggcgtgc tcggctcgga ctacgagctc tggctctatc
     3361 acaaggatgg cggttccggc accgcgatgg tcaaggcggg gacccccgcg cccggcgtgc
     3421 cgcaggtgaa cgcgatgggc gccgccttcg gaaacgatcc ccggtacgtg tacttcgccc
     3481 ggaagcgcgg cgggttcgga tacaacctgc aaatcgacgg cggccaggaa tggcagatcg
     3541 ccgtctacga ccggcagacc ggccagacca acaacgtcac cgacgtcaac ggatccggca
     3601 tgcgccccat cgtgtctccg gacggcaagt ggctggtcta cgcgaatcgg cacgacacgg
     3661 cgaccggctt gcgcctgcgc gatctcgcga ccggcgacga aaagtggctg gtctatccgg
     3721 tgacccgcga cgaccaggaa tcccggttca cccgcgacct cgagcccaac tcggcgttca
     3781 cgccggactc gaaggccctg gtcacgaatt acggcgggaa gatctggcgc gtcgaggttc
     3841 cctcgggaca ggtgagcgcg attccgttca ccgcgcaggt cgagcagcag ctcggtccgc
     3901 tggtccggtt cgacttccgc gtcgacacgg gggagatcac tctccaccag atccgggacg
     3961 cgtccccgtc ccccgacggg aagcgcctgg tcttcagcgc cctcgacaag ctctacgtga
     4021 tggatctgcc caacggcacc ccgcgccgtc tcaccagcgc ggcggtgcac gagcaggaac
     4081 cggtgtggtc ccccgacggg aagtgggtgg cgtacatcac ctggaccgac acgagcggca
     4141 cggtgagcaa ggtatcggcg tcgggcggca atccgacggt gctcaccagc gagccgggct
     4201 tctacgaaca gcccgcgtgg agccccgatg gcaaccggct ggtgttctac aagggtccgc
     4261 gccaggccca cgagacggag catcgcggcg ccggcgaaga tctggtctgg atgccggccg
     4321 ccggcggggc cacgacgcgc atcacgcgga tggaaggcgg cgggcgtccg catttcgccc
     4381 gcgacccgaa ccgcgtgttc gtgtatgacg gaaacgagct ggtttcgatg cgcttcgacg
     4441 gtaccgaccg ccggacctgg atcaaggtga ccggctacac gtatccgggc cccgggtcgg
     4501 agccgtcgac cgccgacgag atcatcatca gccccgactc cgaccgcgtc ctcgcggcgg
     4561 cggggaacaa cgtgtacctc gtgacgctgc ccgaagtcgg gggcacgcca ccgtcgatca
     4621 atatttccga cccgtcctcg gcgtctttcc ccgtgaagcg gctcacgacc atcggcggcg
     4681 acttcatcgg ctgggccccc gacggcaaac gggtattctg gtcgatcggc cggtcgttct
     4741 tccggtatga gccggcggtc gccgactcgc tggagaaggc gaaggccgtg caggactccc
     4801 tccgcaccga gtcgctgaag ggcgatacgc tggcgaacgg gaagcccgac acggtcgcca
     4861 aggcgcgcgt cgactcgctc aacaagaagc ccgcgtacga agccgatcgg tcggacgtga
     4921 cgatcaaggt ggcgcgggac gtgccgaagg gcaccgtggt gctcaagaac gcccgcatca
     4981 tcaccatgaa gggggacgag gtgatcgaga agggtgacct cgtcgtcacc gggaaccgca
     5041 tcgcctgcgt cgggacgtgc acgactcccg ccggcgcgac ggtgatggat ctggcgggga
     5101 agacgatcat ccccggtctg atcgataccc acgcgcaccc ctggccggag tggggcattc
     5161 actcgagcca ggtgtggaag tatctcgcca atctggcctt cggcgtgacc accacgcggg
     5221 acccgcagac ggccacgacg gacgtcctga cctacgccga catggtggag gcggggcaga
     5281 tcatcggtcc ccggatttac cataccggcc cgggcgtctt ttggagcgaa gacatcaaga
     5341 gcctggatga cgcgaagaac atcctgaagc gctacagcga gttctacggt gtgcacacca
     5401 tcaaggagta catgaccggg aaccggaagc agcggcagtg ggtgatcatg gcggcgaagg
     5461 agctgcacct gatgccgacc accgagggcg ggctcgactt caagatgaac ctgacggagg
     5521 ccctggacgg gtatccagga cacgagcaca gctatccgat catgccgctc tacaacgacg
     5581 cggtgcagct gatcgcccag tcgggaatca actacaaccc gacgttgctc gtgaattacg
     5641 gcggtccctt cgccgagaac tacttctatg agcactacaa cattcacgac gatcccaaga
     5701 tccgccgctt cttcccgcac gatcagatcg accagcgggc gctgcgacgt cccgggtggt
     5761 tcgccgacga tcagtatgtc tatcagcaga tcgcggcgag cgcggcgaag atcgttcgcg
     5821 ccggcggcca cgtgggcatc ggctgccacg ggcagttcga cggggaaggg tgccactggg
     5881 agacgtgggc gttcgtggcg ggtgggatga cgccgctcga ggcgctccgg gtcgcgacgc
     5941 atgatggcgc ctatgccatc ggcatggagc aggatctggg gtcgctcgaa ccgggcaagc
     6001 tcgcggatct cgtggtgctc gacgccaatc cgctcgagga catccacaac accacgaaga
     6061 tcagcacggt gatgaagaac ggccggctct atgacggcaa cacgctcgat caggtgtggc
     6121 ccgacaaggt gacgctgccg aggatgtggt ggtggaatca ggagccggga ggcaagaact
     6181 agaaagacgt cgagcaaaga cgtcgagcaa aggcgtcgag cggaggcgta ctaacgcccc
     6241 tgctcgacgc tcttgcttga cgtagctgct tgacgtagtt aggcttctcc tcgaaccgcg
     6301 agctggcatc cttccgaagg acagtcccgc tcaggagcgg gcaccggcgc tgtacacacg
     6361 cccggactca ttccccaggt tcaattcgac gtagtgactc cgccatcgcc ggcactgagg
     6421 ccggaggtgg agtgcgtgtg tgcgaaggag tacgtgtatc atgccgtttg ccgcactcgg
     6481 gctcgtgccc gaattggtgc gcgccgtggc cgaagaaggc tatgagcagc ccacccccat
     6541 tcagcaggaa accatcccgc tcgccctcga ggggcgggac ctcatcggat cggcacagac
     6601 cggtaccggg aaaaccgccg cgttcatgct gcccatcctg cagcggctcg cgaagcacgg
     6661 aacgggcacc cgggcgctgg tcctggtgcc gacgcgcgag ctcgcggagc aggtcgcgac
     6721 gagcgcgcgg gcctacggcc gccatctgcc gctggcggcc gtcgccgtct acggcggcgt
     6781 cgggatggaa ccgcagacca aggcgttgac caggggcgtc gacatcgtcg tcgccacgcc
     6841 gggccgcctg ctcgaccaca tggagcgggg ccacgtcaat ttctcgaagc tcgagatcct
     6901 ggtgctggat gaggccgacc ggatgctcga catgggcttc gcgcccgacg tcaaccggat
     6961 tctccgggcc ctccccggcg aacgccagac gatgctcttc tcggccacag tgtcccgcga
     7021 ggtcgacgcg ctcgcgcgca aggccctgaa cggccatgcg tcggtcgaga tcggccgccg
     7081 ggcgcagacc gccgagggga tcgagcacgt gatcgtggcg gtggacaagg ctcagaagaa
     7141 ggacgtgctg gtcaagatcc tgcaggcgaa gcccgaaggg cagaccctgg tattcacccg
     7201 gaccaagtac ggggccgaca agctcgtcac ccacctgcgc aagaacggca tcggggccca
     7261 cgcgctgcac ggggacaagg cgcagagcca ccggcagcgg acgctcgacg agttccgcga
     7321 cgggcgcacc gaaatcctcg tcgcgacgga catcgcggcc cgggggatcg acgtggacgg
     7381 gatccggatg gtggtgaact tcgacgtgcc gaacgatccc gaagcctacg tgcaccgggt
     7441 gggccgcacc gcccgcgccg gcgctcaggg cctggggctg accctgatct ctcccgatga
     7501 atggctgatg atggccgacg tggagaagct catcggcaag ac
//
LOCUS       DASUBP010000078         2974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_192439, whole genome shotgun sequence.
ACCESSION   DASUBP010000078 DASUBP010000000
VERSION     DASUBP010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192439"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="VFU75_03145"
     CDS             <1..669
                     /locus_tag="VFU75_03145"
                     /inference="COORDINATES: protein motif:HMM:NF023459.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbK domain-containing protein"
                     /protein_id="HEU5465856.1"
                     /translation="NGQYNYIDNIMEAQKVFISELGGTLYTVAKDVKIQVEFNPAQVQ
                     GYRLLGYEDRLLKDEDFKDDKKDAGDMGSGHSVTALYEVIPAGVDADEDLRTPDSLRY
                     QHTKNTLRDPELSKDTGEMLFVKVRYKSPTGSTSKLITHPVLAHSSESPSVDFRFQTA
                     VVEFGLLLRQSQYKGDANLGEVIATARGALGSDPDGYRSEFVRLAQAARGLGLPLDGK
                     DEGR"
     gene            682..1650
                     /locus_tag="VFU75_03150"
     CDS             682..1650
                     /locus_tag="VFU75_03150"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HEU5465857.1"
                     /translation="MLRLPTFRYLAPATLADALKWKVEAGPAGMYVAGGTDLYPNMKR
                     RQQVPATVISLAAIRDLAVLSKTSIGAGNTLAALGESLPPSLSAIATAATSVSTPLLR
                     NMGTLGGNLCLDTRCNYYDQTYEWRKAIHFCMKKDGDTCWVAPSSPRCWAVSSSDVAP
                     VMVAIGAEYQLAGPKGERVVPAARFYHNDGINYLTKQPDEILVAVRLPAEGGWDAVYH
                     KLRRRGSFDFPVLGVAAWVRWNGKSVAEARIVLGGVASYPQEIAEAGAALVGSALEPE
                     AVDKAAEAAFRPAKPMDNTDFDLSWRKQMTRVYVKRALEELRGRHR"
     gene            1673..2968
                     /locus_tag="VFU75_03155"
     CDS             1673..2968
                     /locus_tag="VFU75_03155"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU5465858.1"
                     /translation="MGLAFGLAIGIGGTIGAGILRTPGDLAAYLPSAAWIMGLWIAGG
                     IYCFICSDIFAELGTTIPRSGGLYTFAQRGIGNFAGFLLGYTDAASICAANAALGIVV
                     GEYLGVLIPAVAGHEVLISGIVILTLGALNWAGVRWGSTIQQATTLIKTLAFIALIIA
                     CFVVTPPSGAGAPAPAAFPTGLKFATALLLAMQGVVFTYDSYYYVIYSAEEIENPGRV
                     IPRAIYGSIALIAAIYILLNIAFLRLIPVGAMAGDKFIGGTAAAIIFGPRGDTVIRVL
                     VMASILGTINAYMLAAPRIFRSMAQDGLFAHWATIVNPGGTPTIGLVVGTLASLAFLL
                     TKTFERTLAVGAFFIVFNYALAFLAYFGLRKKEPSLERPYRARHPWTGALALSGAVVF
                     LIAAVTTDTRNSLIALGVVALAWPIFLIVRSTARARPVP"
BASE COUNT          491 a          993 c          947 g          543 t
ORIGIN      
        1 aacggccaat acaactacat cgacaacatc atggaagccc agaaggtctt catctcggag
       61 ctgggcggca cgctgtacac cgtggccaag gacgtgaaga tccaggtcga gttcaatcct
      121 gcccaggtgc aggggtatcg actcctggga tacgaggacc ggctgctcaa ggatgaagac
      181 ttcaaggacg acaagaagga cgcgggtgat atgggttcgg gccactcggt cacggccctg
      241 tacgaggtga ttccggcggg tgtcgatgcc gacgaggatc tgcgtacccc ggactcgctc
      301 cggtatcagc acaccaagaa cactctccgg gaccccgaac tgtcgaagga caccggcgaa
      361 atgctgttcg tgaaggtgcg gtacaagagt ccgacgggga gcaccagcaa gctcatcacg
      421 cacccggtgc tcgcgcattc gtccgagtcg cctagtgtgg acttccggtt ccagacggcc
      481 gtggtcgaat tcggcctcct gctccggcag tctcagtaca agggtgacgc aaacctcgga
      541 gaggtgatcg cgacggcccg cggcgccctg ggctccgatc ccgacggtta tcgctcggag
      601 ttcgtgcggt tggcgcaggc cgcgcggggg ttgggcttgc cgctggacgg aaaggacgag
      661 gggcgttaag gtcggagtcg aatgctgcga ctcccaacgt tcaggtatct cgcgccggct
      721 acgctggcgg acgctctgaa atggaaagtg gaggccggtc ccgccgggat gtacgtcgcc
      781 ggcgggaccg atctttatcc gaacatgaag aggcgccagc aggtccctgc cacggtgatc
      841 tcgctggccg ccatcagaga ccttgccgtc ctttcgaaga ccagcatcgg agcgggcaac
      901 actctggcgg cgctgggcga gtccctcccg ccctcccttt cggccatcgc gacggccgcc
      961 acttccgtgt ccacgcccct gctccgcaac atgggcacac tcggcggcaa cctctgcctc
     1021 gatacccgct gcaactacta cgaccagacg tacgagtgga ggaaggccat ccacttctgc
     1081 atgaagaagg atggcgacac ctgctgggtg gctccgtcga gccccagatg ctgggcggtg
     1141 agctcctcgg atgtcgcacc cgtcatggtc gcgatcggcg ccgagtatca gctggcgggg
     1201 ccgaagggcg agcgggtggt gccggcggcg cgcttctacc acaacgacgg tatcaactac
     1261 ctcaccaagc agccggacga gattctggtg gcggtgcgat tgccggccga aggcgggtgg
     1321 gacgccgtct accacaaatt gcggcgtcgc ggctcgttcg acttcccggt gctcggcgtc
     1381 gcggcgtggg tccggtggaa cgggaagtcc gtcgccgaag cccgcatcgt cctgggcggg
     1441 gtcgcgagct atccccagga gattgctgaa gcgggagccg cgctcgtcgg ctcggccctc
     1501 gagcctgaag ccgtagacaa agcagctgaa gcggcgttcc gccccgcgaa gccgatggat
     1561 aacaccgatt tcgacttgtc gtggcgcaag cagatgaccc gcgtctacgt gaagcgggcc
     1621 ctcgaggagc tgcggggtcg tcatcgctag cccgggcaag ctgctccggg tcctgggtct
     1681 tgccttcggg ctcgcgatcg gcatcggcgg gaccatcggc gccggcatcc tccgcacccc
     1741 gggtgatctc gccgcctacc tcccgagtgc cgcgtggatc atgggcctct ggatcgccgg
     1801 tggcatctac tgcttcatct gctccgacat cttcgccgag ctcggcacca ccattccccg
     1861 ctccggcggt ctctacacct tcgctcaacg aggcatcggc aacttcgccg gctttctcct
     1921 cggctacacc gacgccgcga gcatctgcgc cgcgaacgcc gctctgggca tcgtcgtggg
     1981 ggagtacctg ggtgtcctga tcccggccgt cgccggtcac gaagtcctca tctccgggat
     2041 cgtgatcctg acgctgggag cgctgaactg ggcaggggtc cggtgggggt cgactatcca
     2101 gcaagccacg acgctgatca agaccctcgc cttcatcgcc ctcatcatcg cgtgctttgt
     2161 cgtgaccccg ccttcgggcg cgggggctcc tgctcccgcg gcctttccca ccgggctcaa
     2221 gttcgcaacg gcgctgctgc tcgccatgca gggtgtggtg ttcacctacg acagctacta
     2281 ctacgtgatc tactccgctg aggagatcga aaatccggga cgggtgatcc cgcgcgcgat
     2341 ctacggatcg atcgcgctga tcgcggcgat ctacatcctg ctcaacatcg ccttcttgcg
     2401 gctgattccc gtgggggcaa tggcgggtga caagttcatc ggcggcaccg cggcagcgat
     2461 catcttcggt ccccgcggag acacggtgat ccgggtgctc gtgatggcgt ccatcctcgg
     2521 cacgatcaac gcctacatgc tggcggctcc gcgcatcttc cgctcgatgg cccaggatgg
     2581 cctcttcgcc cattgggcca cgatcgtgaa tcccggaggg acgccaacca tcggactggt
     2641 ggtcgggacc ctcgcatcgc tggcgttcct gctcacgaag accttcgagc ggactctggc
     2701 ggtgggtgcg ttcttcatcg tcttcaacta cgcgctcgcc ttcctcgcgt acttcgggct
     2761 tcgcaagaaa gaaccgtccc tggaacgccc gtaccgcgcc cgccatccct ggacgggagc
     2821 gctcgcactc agcggggcgg tggtctttct gatcgcggcg gtcactacgg acacccgcaa
     2881 cagcctcatc gccctcggcg tcgtcgccct tgcgtggccc attttcctga tcgtccgcag
     2941 caccgctcgc gcccgccccg tcccctgagg ctca
//
LOCUS       DASUBP010000079         3715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_342416, whole genome shotgun sequence.
ACCESSION   DASUBP010000079 DASUBP010000000
VERSION     DASUBP010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3715
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_342416"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="VFU75_03160"
     CDS             <1..472
                     /locus_tag="VFU75_03160"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HEU5465859.1"
                     /translation="PDVIVYPTGGGTGLIGMWKAFNELRDAGWVRGKLPRMFTVQATG
                     CAPVVQAFRDGADHCTPWPNPETVAAGLRVPGPLGDRLMLTALRESSGGAVAVSDQAL
                     TKASDDLQGQEGVDAAPEGGAALAAAVELLATGAIKATERVVIFNTGAGWLYRS"
     gene            500..1690
                     /gene="larC"
                     /locus_tag="VFU75_03165"
     CDS             500..1690
                     /gene="larC"
                     /locus_tag="VFU75_03165"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HEU5465860.1"
                     /translation="MKIAVLDPTAGISGDMTLGALLSLGVPVSWLEGLPSRLGLDGVR
                     VNVRDATRCSVSCKQVEFEIPAQPHGRHVGQLVKLVEQAPLSPAVRQRAVQAFRLLGE
                     AEGRVHGVAPERVHLHEVGAVDAVLDIVGAIEGFERLGVEAIFHRPVAVGDGWVEAAH
                     GRLPVPAPATAILLEGLDVIADGPVHGEATTPTGAALLRVLSIGAPPARWRVTAGGWG
                     AGHRDPKEYPNALRVMLGETADQTEAVTVLATDIDDLSPEYVEPLRQALAEAGALDVQ
                     VWPVQMKKGRPGLRIEIMAPEAQVDAVAAALFRHSPTAGLRRWRAERTTLPRHEIRVE
                     VSIGTASASVRVKVLERPDGVRVKPEYDDVLTAARVLGLPPIDVARAAQREAESRVAQ
                     PHKE"
     gene            1693..3207
                     /locus_tag="VFU75_03170"
     CDS             1693..3207
                     /locus_tag="VFU75_03170"
                     /inference="COORDINATES: protein motif:HMM:NF024821.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5465861.1"
                     /translation="MRRMMLTAVLGLVSVGAGVSPLMGQADWIPAKCDLPGAGQYLVA
                     SAVVYLSKSKDTHFDEVKDRELRDAYRVLNQALTTGQDKNPGAWYYLARYYIERKDMV
                     GMDSSFTKAEALDPKCHDDIYYWRHNNWVPIFNNAVHALNAGKNDSALVYLQQASKAS
                     PAEPDGVSLMGTLFYNGGQYDSAATYFNKGLVIAQDPKFEKSRKDLMFNLAAAEQQLK
                     NYDSAAAVYRGYLKANPNDPSAISQLANTYAAGGHPDSARSLYQQMLQHADGIDPLTL
                     FSAGVAMYNAAPQAPDTQAAGVKCRGDARKVRPALTAAAIKHRCDPPNAQAMAAYDSA
                     AHGTFALAIQAFRAGLAQNPYFRDALYNLTNTYLVTGQKDSMLSTARRLYAVDPMNRK
                     TLQLMAEGFQEQGRTDSALHYITLADSLLPFEVTVGQFQPGDQNVALSGLFTNFHDTP
                     SKPAKVTFEFLDTHGKVIVSQTQDVPAIDASGNAAFQVQAIGANIAAWRYKAGS"
     gene            3209..>3715
                     /locus_tag="VFU75_03175"
     CDS             3209..>3715
                     /locus_tag="VFU75_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU5465862.1"
                     /translation="MSPEGETAGAAGSVPVSPSLAPSLARAGIHGEAPHADRRVLFIG
                     FLAMIVAAAAGVVAQVLIRLIGFITNLSFYGRFSTSFASPSGNHLGWWVLLIPVVGGL
                     IVGFLARYGSAGIRGHGIPEAMEQVLTNQSRIPARLTILKPLSAAIAIGTGGPFGAEG
                     PIIATGGAL"
BASE COUNT          627 a         1223 c         1275 g          590 t
ORIGIN      
        1 gccggacgtc atcgtctatc ccacgggtgg cggcaccggg ctgatcggca tgtggaaggc
       61 gttcaacgag ctgcgggatg ccggatgggt ccgcggcaag ctgccacgga tgttcaccgt
      121 ccaggcgacg ggctgcgcac cggtggtgca ggcattccgg gacggcgccg atcattgcac
      181 gccctggccc aatccggaga cggtggctgc ggggctgagg gttcccggcc cactcggtga
      241 ccgcctgatg ctgacggcat tgcgcgagag tagcggcggg gcggtggccg tatccgacca
      301 ggcgctcacc aaggcgtccg atgatctgca gggccaggag ggtgtcgacg cagcacccga
      361 gggtggcgcg gcgctcgctg ccgccgtcga gctcctcgcc acaggcgcga tcaaggctac
      421 cgagcgcgtg gtcatcttca acaccggcgc cgggtggctc taccggagct aacccgttta
      481 tatttcaggt cttcggacca tgaagatcgc cgttctagac cccaccgccg gcatcagtgg
      541 cgacatgacc ctgggcgctc tgctctcgct cggggtaccc gtgtcatggc tcgaggggct
      601 gccctcgagg ctcgggctgg acggggtccg cgtaaacgtg cgggatgcga cgcggtgcag
      661 cgtttcctgt aagcaagtcg agttcgagat tccggcccag cctcacggcc gccatgtggg
      721 gcaattggtc aagctggtgg agcaggcccc cctgtccccc gcggtccgcc agcgtgcggt
      781 gcaagcgttc aggctcctgg gtgaagcgga agggcgggtc cacggggtgg caccggaaag
      841 ggtgcacctc cacgaagtcg gcgcggtcga tgccgtgctg gacatcgttg gggccatcga
      901 agggttcgag cgcctcgggg tcgaagcgat cttccaccgg ccggtggcgg tgggtgacgg
      961 atgggtcgaa gcggcgcatg gacggcttcc ggtaccggca cccgcgacgg ccattttgct
     1021 tgaaggcttg gacgtgatcg ccgacggccc ggtccacggg gaagcaacga ctcccacggg
     1081 agccgcgctc ctgcgagtgc tgtcgatcgg agctcctccc gcgcgctggc gggtgaccgc
     1141 cggcggctgg ggcgcaggac atcgcgaccc gaaagagtac ccgaatgcgc tccgggtgat
     1201 gctcggcgag accgccgacc agacggaagc ggtgacggta ctcgcgacgg acatcgacga
     1261 tctgagtccc gagtatgtcg agcccctccg ccaggccttg gcggaagcgg gtgccctcga
     1321 cgtgcaggtg tggccggtgc agatgaagaa ggggaggccg ggtctccgga tcgagatcat
     1381 ggctcccgaa gcgcaggtgg acgccgtcgc tgccgctctg ttccggcaca gcccgacggc
     1441 gggtctgcgc cgctggcgcg ctgaacgcac cacgctgccg cggcacgaga tccgcgtgga
     1501 ggtgtcgatc ggcacggctt ccgcgagcgt tcgcgtgaag gttctggaac gtccggacgg
     1561 agtccgggtc aaacccgagt atgacgatgt gctgacggcg gcccgagtgc tggggctgcc
     1621 ccccatcgac gtggcacgcg cggcacagcg agaagccgaa tcgcgcgtgg cccaacctca
     1681 taaggagtag cgatgcgacg gatgatgctg acggcggtac tgggtctcgt ttccgtgggc
     1741 gcgggggtga gcccgctcat ggggcaggcg gactggatcc cggccaagtg cgacctcccc
     1801 ggcgcggggc aatatctggt cgccagcgcg gtcgtctacc tgagcaagtc gaaggacacg
     1861 cacttcgacg aggtgaagga ccgcgagctt cgcgacgcct accgcgtact caaccaggcg
     1921 ctcacgacgg gtcaggacaa gaaccccggt gcctggtact acctcgcgcg ctactacatc
     1981 gagcggaagg acatggtcgg catggactcg tccttcacca aggccgaggc gctggacccg
     2041 aaatgccatg acgacatcta ctactggcgc cacaacaact gggtgccgat tttcaataac
     2101 gccgttcatg ccctgaacgc cgggaagaac gactcggcgc tcgtgtatct ccagcaggcg
     2161 agcaaggcga gccccgccga gcccgacggc gtctcgctca tgggcacgct cttttacaac
     2221 ggcggccagt acgactcggc ggcgacgtat ttcaacaagg gcctggtcat cgcgcaggat
     2281 ccgaaattcg agaagagccg caaggacttg atgttcaacc tcgcggcggc cgagcagcag
     2341 ctgaagaact acgattcggc ggccgccgtg taccgcggct acctcaaggc caaccctaac
     2401 gacccgtccg cgatctccca gctggccaac acgtatgcgg cgggtgggca tccggattcg
     2461 gctcgctctc tgtatcagca gatgcttcag cacgcggatg gcatcgaccc gctcacgctg
     2521 ttctcggccg gggtcgcgat gtacaatgcc gcaccgcagg cgcccgacac ccaggcggcc
     2581 ggcgtgaagt gccggggcga cgcgcggaag gtccgtccgg cgctcaccgc cgcggcgatc
     2641 aagcaccgtt gcgatccacc caacgctcag gcgatggccg cgtacgactc tgcggcccac
     2701 gggacgttcg ccctcgcgat ccaggcgttc cgggcgggac ttgcgcagaa cccgtacttc
     2761 cgggatgcac tctacaacct gacgaacacc tatctggtga ccgggcagaa ggacagcatg
     2821 ctgagcacgg cgcgccggct gtacgccgtc gaccccatga accgcaagac cctccagctc
     2881 atggcggagg ggttccagga acaggggagg accgattcgg cgctgcacta catcacgctc
     2941 gccgattcgc tgctgccgtt cgaagtgacg gtgggccagt tccagccggg cgaccagaac
     3001 gtggcgctca gcgggctctt caccaacttc cacgacacgc cgagcaagcc cgcgaaggtc
     3061 acgttcgagt ttctcgacac gcacggcaag gtgatcgtca gccagacgca agacgtgccg
     3121 gcgatcgacg cgagcggcaa cgccgcgttc caggtccagg cgatcggcgc gaacatcgcc
     3181 gcgtggcggt acaaggcggg gtcgtgacgt gagccccgaa ggggagacgg ccggggcggc
     3241 cggaagcgtc ccggtctctc cctctctcgc gccgtcactg gcgcgggcgg gcattcacgg
     3301 agaagctccg cacgccgacc ggcgcgtact gttcatcgga tttctggcga tgatcgtggc
     3361 ggcggcggcg ggcgtcgtcg ctcaggtcct gattcgcctc atcggcttca tcaccaatct
     3421 cagcttctac ggacggttct cgacctcgtt cgcgtccccc tccggcaacc atctcggctg
     3481 gtgggtgctc ctcatcccgg tcgtcggcgg cctgatcgtc ggattcctcg cgcgttatgg
     3541 atccgccggc atccgcggtc acgggatccc cgaggcgatg gagcaggtcc tcaccaatca
     3601 gagccggatt cccgcgcgcc tcacgattct caaaccgctc tccgccgcga tcgccatcgg
     3661 caccggtggc cccttcggag ccgaagggcc gatcatcgcg acgggtgggg cactc
//
LOCUS       DASUBP010000080         4540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_696764, whole genome shotgun sequence.
ACCESSION   DASUBP010000080 DASUBP010000000
VERSION     DASUBP010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4540
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_696764"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            30..794
                     /locus_tag="VFU75_03180"
     CDS             30..794
                     /locus_tag="VFU75_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HEU5465863.1"
                     /translation="MGIFDRLSTLIRSNINDLISRAENPEKMLNQLIVDMRTQLAKAK
                     QQVASAIADEKKLSAQAEQEKKMAEDWEKRAVLAVQEGRDDLAKQALLRHNEHVQNAL
                     TLHDTWVKHREETEKLKLSLRQLNDKIEEAKRKKNILIARQKRAEAHKRIQETMSSMG
                     DNSAFETFERMAEQIEHEERKLIAAAEVNEDLSGDSLMKQFQTIEVRADADQQLAALK
                     QKMGLLGAGSPAETKQIGKGAKDAELVEDDEAGHTH"
     gene            842..1930
                     /locus_tag="VFU75_03185"
     CDS             842..1930
                     /locus_tag="VFU75_03185"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU5465864.1"
                     /translation="MALFVAALGIWFAVRVVDVLIVIFIAILFSIYLGAVTDWLERRL
                     NVPRWAGLTIAVTGTLGAVVGLGAVLLPPVIDQTQALVSGLPGTLTEVQNVLARTAAR
                     YPVLRRSPLADPTSGVVGSLITEGFGFLRTSVLPYLRAGGLALIEGVSIFVMALYLAR
                     SPGVYRDGVIGLVAPQHRVTAAHILDDTAQTLRAWVGGQLLAMVVLGTLTTIGLLILG
                     APYWLAFGVFAGVAAIVPFFGTLLSTVLPALFVIGEGDWLRVVAVLLLGVVVHIIEAN
                     VVGPLIMERKVALPPVLTIASVLVMGTLLGIVGLVVAVPILATVIVFVRHVVHGEIYG
                     DVATQSAVLRHTDEFRIPLHLPKRRPER"
     gene            1927..3012
                     /locus_tag="VFU75_03190"
     CDS             1927..3012
                     /locus_tag="VFU75_03190"
                     /inference="COORDINATES: protein motif:HMM:NF019375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HEU5465865.1"
                     /translation="MTRPDHRFLILADGQFDPLTSKTANSCVRYFPERIVGVFDRRHA
                     GRTVQDVLGFGGTIPVVGDFAKGMALGGGATAILIGIAPAGGQLPDEWRGWLKTAIES
                     GLEVWSGLHTFIGDDPELGAIAKKKGVRILDARKPPAHLPIADGRAAEVSALTVLAVG
                     SDCNVGKMTAQLQLRDALVKRGRRTSFVATGQTGIFIEGWGIAVDAVVADFIAGAAES
                     LVLQGAKDHDIVLVEGQGSLIHPGYSGVTLGLLHGSCPDAMILCHQSSRDFIGDYHGR
                     APWVKIPPLPELIRVYEDAARPVRESKVIAVCLNTFDLDERAAREAVARATKETGLPA
                     TDPVRFDPAPVVDAMIAAADEKKKHRH"
     gene            3087..3554
                     /locus_tag="VFU75_03195"
     CDS             3087..3554
                     /locus_tag="VFU75_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465866.1"
                     /translation="MTTFLFFRQALAEWRPVRALVRETRLRLWLAVIGLGAVGLSGLD
                     DGVSLPVLTLGVALTATVAAAAVLAGPHAGRVTAMTLRHPASPLALALGRWLAVTGLA
                     GVVTLGAGVGAAWRVELGWRAGVDAALSAAGVTVPVAACGLLVTAELWRRWKP"
     gene            3551..4411
                     /locus_tag="VFU75_03200"
     CDS             3551..4411
                     /locus_tag="VFU75_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5465867.1"
                     /translation="MIPPAISLSQVTWVGRTGRVLDGVDAEVIPGEVVAVVGGAGTGK
                     STMLRVAAGIARAHAGQARVGSWDAKDARVRKIVGYAPQDPAWLAAFTVRELLEYWGR
                     FHVRGSPVTAVRDALAVSGLELHADDPISAADVSVRRRLALAQAVLGGRRVLVLDELL
                     TGLDPAVRQELAWRLESRAAAGVAILLSAGSLEAVERVATRVLVLRHGRVARVAAPTA
                     LLAERVLDVILDRPALAAPSGFRLTPFGLQVDLSRVTPEAALATCRAYRLSVRASHVR
                     AKSLEELAVS"
BASE COUNT          672 a         1532 c         1558 g          778 t
ORIGIN      
        1 cgggtcgctg acccgcaata gaggggtgta tggggatctt tgaccgtctc tcgacgctca
       61 tccggtcgaa catcaacgac ctgatttcgc gcgccgagaa tcctgagaag atgctgaacc
      121 agctcatcgt cgacatgcgg acccaactgg ccaaggcgaa gcagcaggtg gcctccgcga
      181 ttgccgatga aaagaagctc tccgcccagg ccgagcagga gaagaaaatg gccgaggact
      241 gggagaagcg cgccgtgctg gcggttcagg aaggccggga cgacctcgcc aagcaggcgc
      301 tcctgcggca caacgagcac gtccagaatg ccctcaccct gcacgacacc tgggtgaagc
      361 accgggaaga gaccgagaag ctcaaactct cgctgcgcca gctcaacgac aagatcgaag
      421 aggcgaagcg gaagaagaac atcctgatcg cgcggcagaa gcgggccgaa gcccacaagc
      481 gcattcagga gaccatgtcg tcgatgggcg acaactcggc cttcgagacc ttcgagcgca
      541 tggccgagca gatcgaacac gaggagcgga agctcatcgc ggccgccgag gtcaacgaag
      601 atctgtcggg tgactccctg atgaaacagt tccagaccat cgaagtccgc gccgacgcgg
      661 atcagcagct cgcggccctc aagcagaaga tgggcctgct cggcgccgga tcccctgccg
      721 aaaccaaaca gatcggcaag ggcgcgaaag acgccgagct ggtcgaggac gacgaagccg
      781 ggcacaccca ctgaactccg ctgagccgtc ggcgaagtcc aaactctcgg gcgccacgtt
      841 cgtggcgctc ttcgttgccg ccctggggat ctggttcgcc gtccgggtcg tcgacgtcct
      901 catcgtcatc ttcatcgcga tcctgttttc catctacctc ggcgcggtca ccgactggct
      961 ggagcgccgc ctcaacgtcc cgcgctgggc cggactgacc atcgccgtga ccggtaccct
     1021 cggcgccgtg gtcggtctgg gggcagtgct gctcccgccg gtgatcgacc agacgcaagc
     1081 actcgtctcc ggactgcccg ggaccctcac cgaggtccag aacgtcctgg cccgcacggc
     1141 ggcgcggtac ccggtgctgc gccggtcacc cctcgccgat ccgacctcgg gagtggtggg
     1201 ctcgctcatc acggaaggat tcggatttct gcgaacctcc gtcctcccat acctccgggc
     1261 cggcggcctc gcgttgatcg aaggggtcag catcttcgtc atggcgctct atctggcgcg
     1321 cagtcccggg gtgtaccgcg acggcgtcat tggcctggtg gcgcctcagc atcgggtcac
     1381 cgccgcgcac atcctcgacg acacggcgca gacgctgcgc gcctgggtgg gcggccagct
     1441 gctggcgatg gtcgtcctcg gcacgctgac gacgatcggc ctcctgatcc tcggcgcccc
     1501 gtactggctc gcgttcggcg tcttcgcggg cgtcgccgcc atcgtcccct tcttcgggac
     1561 cctcttgtcc accgtgcttc cggccctgtt cgtaatcggg gaaggggact ggttgcgtgt
     1621 ggtcgcggtc ctgctcctgg gagtggtggt ccacatcatc gaggccaacg tggtaggccc
     1681 cctgataatg gaaaggaaag tggccctgcc tcccgtcctc accatcgcca gcgtgctggt
     1741 gatggggacc ttgctcggga tcgtagggct ggtcgtggcc gtccccattc tcgcgaccgt
     1801 gatcgtcttc gtccggcacg tggtccatgg cgagatctac ggggacgtcg ccacccagtc
     1861 cgcggtgctg cggcatacgg acgaattccg catccccctc caccttccca aacgacgtcc
     1921 cgagcgatga cgcgccccga ccaccggttc ctgattctcg ccgacggcca attcgatccc
     1981 ctcacctcga agaccgccaa cagctgcgtc cggtacttcc cggaacgcat cgtgggcgtg
     2041 ttcgaccggc gtcacgccgg cagaaccgtt caggatgtgt tggggttcgg cggcaccatc
     2101 ccggtggtcg gggatttcgc gaagggcatg gcgctcgggg gcggggcgac ggcgattctc
     2161 atcgggatcg cgccggcggg gggccagctt cccgacgaat ggcgtgggtg gctcaagacc
     2221 gcgatcgaaa gcgggctcga ggtctggagt ggtctccaca ccttcatcgg cgatgatccg
     2281 gagctggggg cgatcgcgaa gaagaaaggc gttcgaatcc tcgacgcccg gaagcctccc
     2341 gcccatctcc ccatcgcgga tggccgcgcg gcggaggtct cggcgctcac cgtgctcgcc
     2401 gtgggctccg actgcaacgt cggcaagatg accgctcagc tccagctgcg ggacgcactg
     2461 gtgaaacggg gccggcgaac cagcttcgtg gccaccgggc agaccgggat cttcatcgag
     2521 ggatggggaa tcgcggtgga cgccgttgtc gccgacttca tcgccggcgc cgccgagtca
     2581 ctcgtgctgc agggggccaa ggatcatgac atcgtgctgg tcgaggggca gggcagtctc
     2641 attcaccccg gctactccgg tgtcacgctc ggactgctgc acggctcctg tccggacgcg
     2701 atgattctgt gccaccagtc atcccgcgac ttcatcggcg attatcacgg ccgcgcgccc
     2761 tgggtcaaga ttccgccgtt gccggagctc atccgggtat acgaggacgc cgctcgcccg
     2821 gtgcgcgaat ccaaggtgat cgccgtgtgc ctgaacacgt tcgatctcga tgaacgcgcc
     2881 gcgcgtgagg ccgtagcacg cgcgaccaag gagacggggc tcccggccac cgatcccgtt
     2941 cgcttcgatc cggcgccggt ggtcgacgcg atgatcgccg cggctgacga gaagaagaag
     3001 caccggcact aggtccggcg tttttcgggc ccttcgggcg tcgatcggat cacggtcggc
     3061 gcccgcttct tttgtagata gggcgcgtga cgacgttcct gtttttccgt caagccctcg
     3121 ccgagtggag gcccgtgcgc gcgctggtcc gcgagacccg gctgaggctc tggctggcgg
     3181 tcatcgggct cggggccgtg ggtctcagtg gactcgacga tggcgtgtcc ctgccggtat
     3241 tgaccctcgg cgtcgcgttg accgccaccg tggccgccgc cgccgtcctc gccggacccc
     3301 acgcggggcg cgtcacggcg atgacgttgc gtcatccggc gtcacccttg gcgctcgccc
     3361 tgggacgctg gctcgccgtg accgggttgg cgggagtggt gacgctgggt gcgggtgtgg
     3421 gcgccgcctg gcgcgtggag ttggggtggc gagcgggtgt ggatgcggcg ctctcggcgg
     3481 cgggtgtcac cgtcccggtc gcagcgtgcg gactcctcgt cacggcggaa ctctggcgcc
     3541 ggtggaagcc atgatcccgc ccgcgatttc cctctcccag gtcacctggg tgggacggac
     3601 gggccgggtg ctcgacgggg tggacgccga ggtgattccg ggtgaggtgg tcgccgtcgt
     3661 cggcggggcc ggtacgggga agtcgacgat gctgcgcgtc gccgccggca ttgcgcgcgc
     3721 ccacgcgggg caggcgcggg tgggatcgtg ggatgcgaag gacgcccgcg tccgcaagat
     3781 cgtcggatat gcgccgcagg acccggcgtg gttggccgcc tttaccgtcc gggagctgct
     3841 ggagtactgg ggccggtttc acgtgcgcgg cagtcccgtc acggcggttc gcgatgccct
     3901 cgccgtctcc ggactcgaat tgcacgccga cgaccccatc agcgcggccg atgtctccgt
     3961 ccgccggcgg ctggcgctgg cccaggccgt gctggggggc cggcgcgtgc tcgtactcga
     4021 cgagcttctc accggcctcg atcccgccgt tcggcaggag ctggcctggc ggctcgaatc
     4081 ccgggccgcc gccggcgtcg cgatcctgct ttcggcgggc tcgctcgagg cggtggagcg
     4141 ggtggcgacc cgggtgctgg tgctgcgtca tggccgggtg gcgcgggtcg ccgcgcccac
     4201 ggcactcctg gcggaacggg ttctggatgt gatcctcgac cggccggccc tggcggcgcc
     4261 gtcggggttc cgcctgacac ccttcggact gcaggtagac ctctcccggg tgacgccgga
     4321 ggcggccctt gccacgtgcc gtgcctaccg cctctcggtc cgggcctccc acgtccgcgc
     4381 caagtctctg gaggagcttg cggtgagtta attttctccg catggccctc ttcctccata
     4441 acacgctcac gcgcgccatc gagccttttc agccgctcca cccgccgcgg gtgacgctgt
     4501 atacgtgcgg cccgaccgtc tggaactacg cgcacatcgg
//
LOCUS       DASUBP010000081         4670 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_56283, whole genome shotgun sequence.
ACCESSION   DASUBP010000081 DASUBP010000000
VERSION     DASUBP010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4670)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4670)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4670
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56283"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..77)
                     /locus_tag="VFU75_03205"
     tRNA            complement(5..77)
                     /locus_tag="VFU75_03205"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(42..44),aa:Ala,seq:ggc)
     gene            243..1262
                     /locus_tag="VFU75_03210"
     CDS             243..1262
                     /locus_tag="VFU75_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HEU5465868.1"
                     /translation="MTKNEKKKKSAVTKKAPAKKAVAPKAAAKTPAAPAPAAAPAPKA
                     KATKKRGRRRGLLTGLGHHEHERDILDQYLHEVSKTPLLTQKEEIALARKVRAGDQEA
                     MQELVKRNLRFVISVAKKYQNRGLPLTDLIGEGNVGLLTAARKFDPDQGVKFISYAVW
                     WIRQAILASLARQGRTVRVPLNRTADLSRIVRTAEYLRQTLRREPTPEEIADATKLSL
                     EVVQSLAALNTGDVRLDAPLDPDGDRSLIERFIAEDLPDTEDQAMDRFLSDEVENALN
                     TLQRRDAKVLRLYFGLDGGREHTLEEIGGLLGVTRERVRQLRDRALKRLRDGDVGKAL
                     GSFAA"
     gene            1382..2143
                     /locus_tag="VFU75_03215"
     CDS             1382..2143
                     /locus_tag="VFU75_03215"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5465869.1"
                     /translation="MITALGRNTAATVRSVGRLGQFLSDTVRALPDVGIWFPLTITQM
                     RRVGVDSLPVALFIAAFTGIVLALQASYTFTGTVPLYFVGTLVGKTMMLELGPVLAGL
                     ALAGRVGASMAAELGTMRVTEQVDALETLAYDPQSFLVIPRIVAGTTMFPVIVAFAIA
                     VGITTGWLTSLGLLGLSTPDFLKGLKLFYVFKDVWYGLFKSATFGFTITLVGCVVGLG
                     TRGGAEGVGLSTTRAVVYSAVLLLVLDAFWAMTIL"
     gene            2140..2865
                     /locus_tag="VFU75_03220"
     CDS             2140..2865
                     /locus_tag="VFU75_03220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5465870.1"
                     /translation="MIELKGVRKAFGEHVVLDDCTLTVRDRETMVVIGYSGTGKSVLL
                     KHVVGLLLPDAGQVIVDGQSVPDLNRHGLSNLRAQIGYVFQAAALFDSMTVAENVALG
                     LRRRALADDEIAERVSEALALVDLTGNDDKLPAELSGGMRKRVGIARAIALRPRYILY
                     DEPTTGLDPVTSAVIDQLMVRTREHLGVTGIVVTHDMRSAYTVGDRIAMLYEGKIRQV
                     GSVAEIQATQDPLVRQFIEGIPS"
     gene            2862..3869
                     /locus_tag="VFU75_03225"
     CDS             2862..3869
                     /locus_tag="VFU75_03225"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEU5465871.1"
                     /translation="MKRENEFAVGLAVIAALTLVVGGALWLSGAHLGKTETMYTSRFR
                     TVGGLGVGNPVVLRGVRIGRVEAIRLAPGNWVEADLKVYTGVSLPPRPAAIAASASLF
                     GEWAVTVTSQDQLPDDPNVRSAIAEAAAGGGGRWPGATLPDIGQLTAQASRIATDIGT
                     VANRIQTAFDTNAVNDLRRSIHDFGQIADRLVKVTDEQATVIGNVGNNLQTGSKSLAD
                     AATRLQTTAGRLDSATSGGQVSSILNNTQAASQDIRTTSADLRDLAEAAHKNQESLVR
                     VLVNADTVMSRIANKTGTLGLLVADSSLYKETTFTMIQLRQLLADIQANPRKYFTFSV
                     F"
     gene            3877..4341
                     /locus_tag="VFU75_03230"
     CDS             3877..4341
                     /locus_tag="VFU75_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU5465872.1"
                     /translation="MSKRALKAQRETSAGGVVFRREEGVARYLLIRDSYKNWGFPKGH
                     LKTAEPPADAARREVEEETGLKDLLLHGPIRVIDWYFRFKGKTIHKYCHFFLFESRSG
                     EPTPQLEEGISACSWHPLDDAVSTISYDNARGVLRRGAEMVRALQEVPEPPR"
     gene            4338..>4670
                     /locus_tag="VFU75_03235"
     CDS             4338..>4670
                     /locus_tag="VFU75_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465873.1"
                     /translation="MTGVVPVYHPRADARRAVRRGIPTGAGGGVRVCRSADQLRRLML
                     QRLIGAVVLDVKSVPADVFALPARYPRIPFYALSAFKPEDGPILARCRDSGFAGVLVE
                     GVDNAVAGE"
BASE COUNT          782 a         1593 c         1519 g          776 t
ORIGIN      
        1 tcagtggagg cgagggggat cgaacccctg acctcgtgaa tgccattcac gcgctctccc
       61 aactgagcta cgcccccctt gtttttccag ccttctcagg gatatttgcg gccccccgac
      121 aaagcccctg ggcgggaaca cggcatctta tcggggcgtt atgtcagtgt caagcgatat
      181 cgggccttcg gtgaactttc cgggccctca ggcgtactag aggctcgacc gagaccaatc
      241 ccatgaccaa gaacgaaaag aagaagaaaa gcgccgtgac caagaaggca ccggccaaaa
      301 aggccgtggc gccgaaggcc gcggccaaga ctcccgctgc gcccgctccc gcggccgccc
      361 cggcccccaa ggccaaggcg accaagaagc gcgggcgccg ccgcggcctc ctcaccggcc
      421 tcggccatca cgagcacgag cgcgacatcc tcgatcagta cctgcacgag gtctccaaga
      481 ctcccctgct cacgcagaag gaggagatcg ccctcgcccg gaaggtgcgc gccggcgatc
      541 aggaggccat gcaggagctg gtcaagcgca acctgcgctt cgtcatctcc gtggccaaga
      601 aatatcagaa ccgcggtctc ccgctcaccg acctgatcgg cgaggggaac gtcggactgc
      661 tcacggccgc gcggaagttc gatcccgacc agggcgtgaa gttcatctcc tacgccgtat
      721 ggtggatccg ccaggcgatc ctcgcctcgc tggcccgcca gggtcgcacc gtgcgcgtgc
      781 cgctgaaccg caccgccgat ctctcccgca tcgtccgcac cgccgagtac ctgcggcaga
      841 cgctgcgccg ggagccgacg cctgaagaga tcgccgacgc caccaagctg tcgctcgagg
      901 tcgtgcagtc cctcgccgcc ctcaacacgg gtgacgtgcg cctcgacgcg ccgctcgatc
      961 ccgacggtga ccgctcgctc atcgagcgct tcatcgccga ggatctgccg gacaccgagg
     1021 accaggcgat ggaccggttc ctctcggacg aagtggagaa cgcgctgaac accctccagc
     1081 gccgcgacgc caaagtcctg cgcctgtact tcggcctgga cggcggacgc gaacacaccc
     1141 tcgaagagat cggcggcctc ctcggcgtca ctcgggagcg ggtgcggcag ctgcgcgacc
     1201 gggccctcaa gcggctccgg gacggagacg tcgggaaggc gctggggagc tttgctgcat
     1261 agtcgctaag acgctaagtc gttaagtcgc taagaagagg ccggggagac aaatcctcgg
     1321 cctctttcgc tttcactata ccgacttagc gacttggcga cttagcgtct attttccggc
     1381 cgtgatcacc gccctcggcc ggaacaccgc cgccaccgtg cgctccgtgg gccgtcttgg
     1441 ccagttcctc tccgacaccg tccgggccct ccccgacgtc ggcatctggt tccccctcac
     1501 catcacgcag atgcgccgcg tgggagtcga ctccctcccc gtggccctct tcatcgccgc
     1561 cttcaccggc atcgtcctcg ccctccaggc ctcctacacg ttcaccggca ccgtgccgct
     1621 ctatttcgtg ggcaccctgg tgggcaagac catgatgttg gagctcggtc cggtgctcgc
     1681 cggcctggcc ctcgccgggc gggtgggtgc cagcatggcc gcggagctcg ggaccatgcg
     1741 cgtgaccgaa caggtggatg cgctcgagac cctcgcctac gacccccaga gtttcctggt
     1801 catcccgcgg atcgtcgcgg gcaccaccat gttccccgtg atcgtcgcct tcgccatcgc
     1861 cgtcggcatc accaccgggt ggctcacgtc tctgggcctc ctgggtctct cgacgccgga
     1921 ttttctcaag ggcctcaagc tcttctacgt gttcaaggac gtgtggtacg gcctcttcaa
     1981 gagcgcgacc ttcggattca ccatcaccct cgtgggctgc gtggtgggac tcggcacccg
     2041 ggggggcgcg gaaggggtgg gattgagcac cacgcgcgcg gtcgtgtact ccgccgtcct
     2101 gctcctcgtc ctcgacgcct tctgggcgat gaccatcctg tgatcgagct caagggcgtc
     2161 cggaaggcct tcggtgagca cgtggtactc gacgactgca cgctcaccgt ccgggatcgt
     2221 gagacgatgg tcgtcatcgg gtactccggt accggcaaga gcgtgctcct gaagcacgtg
     2281 gtcggactgt tgctccccga cgccgggcag gtcatcgttg acgggcagtc ggtgccggac
     2341 ctgaatcggc acggcctgtc gaatctgcgg gcgcagatcg gctacgtgtt ccaggccgcg
     2401 gccctcttcg attccatgac ggtcgcggag aatgtcgccc tgggactcag gcgccgggcg
     2461 ctggccgacg atgaaatcgc cgaacgggtg tccgaagcgc tggccctggt ggatctgacg
     2521 gggaatgacg acaagttgcc cgccgagctg tccgggggaa tgaggaaacg ggtcgggatc
     2581 gcgcgcgcca tcgcgctgcg gccacgctac atcctctatg atgagccgac caccggcctg
     2641 gatcctgtca cctcggccgt gatcgatcaa ttgatggtgc gcactcggga gcatctgggc
     2701 gtcaccggca tcgtggtgac ccacgacatg cggagcgcct atacggtggg ggaccggatc
     2761 gcgatgttgt atgaaggaaa gatccggcag gtcggatcgg tcgccgaaat ccaggcgacc
     2821 caggatcccc tggtccggca gttcatcgaa gggattcctt cgtgaagcgc gaaaacgagt
     2881 tcgcggtcgg tctggcggtc atcgccgcgc tcaccctggt ggtgggtggg gcgctctggc
     2941 tgtcgggcgc gcacctcggc aagaccgaga cgatgtacac ctcgcgcttc cgcaccgtgg
     3001 gtggcctggg cgtcggcaat ccggtggtgc tccgaggcgt ccggatcggc cgggtggagg
     3061 cgattcgcct cgcgccgggg aactgggtcg aagccgacct gaaggtctat accggggtgt
     3121 cgctcccccc gcgccccgcc gccatcgccg cctcggcgtc cctgttcggc gaatgggccg
     3181 tcaccgtcac gtcccaggac cagctccccg atgatcccaa cgttcgctcg gccatcgccg
     3241 aagcggcggc gggcggggga gggcggtggc ccggcgcgac gttgcccgac atcggccagc
     3301 tcacggcgca ggcctcgcgc atcgcgaccg atatcggcac cgtcgccaac cgcatccaga
     3361 ccgccttcga tacgaacgcc gtcaatgacc tgcggcgctc gatccacgac ttcggccaga
     3421 tcgccgaccg gctggtgaag gtgaccgacg agcaggccac cgtgatcggc aacgtcggca
     3481 acaatctgca aaccggctcg aagtctctcg ccgacgcggc cacgcgactc cagaccaccg
     3541 ccggacggct ggacagcgcg acgagcgggg ggcaggtatc ctccatcctg aacaacactc
     3601 aggcggcgag tcaggacatc cgcaccacca gcgccgatct gcgtgatctg gctgaggcgg
     3661 cgcacaagaa ccaggagagt ctggttcggg tactggtaaa cgcggacacc gtgatgtcgc
     3721 ggatcgccaa caagaccggc acactggggc tcctggtggc cgattcgtcg ctctataagg
     3781 aaacgacctt tacgatgatt cagcttcgcc agctcctggc cgacatccag gccaatccgc
     3841 gcaaatactt cacgttcagc gtcttttagg gaccagatgt cgaagcgggc gttgaaggcg
     3901 cagcgggaga cgagcgcggg gggcgtggtg ttccgtcggg aggagggggt cgcgcgatac
     3961 ctcctcatcc gggattccta caagaactgg ggctttccga aggggcacct gaaaaccgcg
     4021 gagcctcccg cggacgcggc tcgacgcgaa gtcgaggaag aaaccggcct gaaggatctg
     4081 ctgctccacg ggcccatccg ggtcatcgac tggtacttcc gcttcaaggg caagaccatc
     4141 cacaagtatt gccacttctt cctcttcgag tcccggtccg gagagccgac gccgcagctc
     4201 gaagagggga tctccgcgtg ctcgtggcat ccgctggatg acgcggtctc cacgatctcc
     4261 tacgacaacg cgcggggcgt gctccgacgg ggagcggaga tggtgcgagc cctgcaggag
     4321 gtgccggagc cgccgcggtg acgggggtgg tcccggttta ccaccctcga gccgacgcgc
     4381 ggcgtgccgt gcgccggggc attccgaccg gcgccggcgg aggtgtccgc gtgtgccgga
     4441 gcgcggacca actgcggcgt ctcatgcttc agcgtctcat cggggccgtg gtcctcgatg
     4501 taaagtccgt gccggccgac gtcttcgctc tccccgcccg ctaccccaga atcccgttct
     4561 acgcgctctc ggcgttcaag cctgaggacg gcccgatcct cgcgcgttgc cgggactcag
     4621 ggttcgccgg cgtgctggtc gaaggcgtcg acaacgcggt ggcgggggag
//
LOCUS       DASUBP010000082        13643 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_587907, whole genome shotgun sequence.
ACCESSION   DASUBP010000082 DASUBP010000000
VERSION     DASUBP010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 13643)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13643)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13643
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587907"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1419)
                     /gene="clpX"
                     /locus_tag="VFU75_03240"
     CDS             complement(166..1419)
                     /gene="clpX"
                     /locus_tag="VFU75_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEU5465874.1"
                     /translation="MSHDKHLRCSFCGKSKDSVRKFISGPSVYICNECIALCNEILAE
                     DEEREVAEAITQVPTPQEIKDVLDQYVIGQEKAKKTLAVAVYNHYKRINSHSNGRESE
                     VELDKSNILLIGPTGVGKTLLAQTLARILDVPFTIADATTLTEAGYVGEDVENILVRL
                     LQAGDFNVTECERGIVYIDEIDKIARKSENPSITRDVSGEGVQQALLKILEGTVASVP
                     PQGGRKHPQQEYIQVNTKDILFICGGAFDGLEKIIESRTGRRQIGFSKEEVAKGEADK
                     RKKNPFVDVEPDDLLRYGLIPELVGRLPVTVPLDALDEESLVRILVEPKNALTKQYAK
                     LFELEDVRLTIDKDALKAIAQKAIKRGTGARGLRAILEEMMTEIMFDLPGRDDVREVA
                     ITAECVTAGRQPLLVTERPRQKKEA"
     gene            complement(1460..2077)
                     /locus_tag="VFU75_03245"
     CDS             complement(1460..2077)
                     /locus_tag="VFU75_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HEU5465875.1"
                     /translation="MTSGTIYPPYIIERSSRGERTYDIFSRLLMDRIVFLGTAIDDTV
                     ANIIIAQLLFLDADNPERDIYLYINSPGGLVSSGMAIYDTMQFLRAPVNTICMGMAAS
                     MGSFLLTAGTTGKRSALPHARIMMHQPSGGASGTAADIEIQAREIIYLRGKLNELYSK
                     HTKKPLEQIEKDMDRDRFMSAEEAKEYGLIDNVIAQLKDLDGKKA"
     gene            complement(2074..3318)
                     /gene="tig"
                     /locus_tag="VFU75_03250"
     CDS             complement(2074..3318)
                     /gene="tig"
                     /locus_tag="VFU75_03250"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEU5465876.1"
                     /translation="MTIEITKTSSEPGAASLAVSVPEENVRAAEERATRAYQSRARLP
                     GFRKGKAPSAVVKKRFAEDIRQQALQEILQESWKAAVDQEKLKPIADPHIHNLKWEQG
                     TPVTFEFHVEVKPELKLERLGGFTLKRTVSKVSADQVEAQINDLRDQKAPWIPLAGEK
                     PKPKDLVHITLAQREGEEIKDTQPYQFVLGEGRAIPDVEEKVMSLLPGETVDATVKFP
                     ADFPEEAKRGQSREVRITLTEVKRQELPALDDAFAREVGDFDSVEALRKAVREDLEKE
                     ADRQADANVRAALIEQIVAANSVVAPAPLVNRALAVYAQAYGIPEDRWEQFSTEMRSV
                     AEQQVRRDLILDYVVEANTLAATEAELDAKIEELAAKRGQPARELYASLEKAKRLRDL
                     ERGLTEEKVFTFLLSQSTVENA"
     gene            complement(3460..4563)
                     /gene="ugpC"
                     /locus_tag="VFU75_03255"
     CDS             complement(3460..4563)
                     /gene="ugpC"
                     /locus_tag="VFU75_03255"
                     /inference="COORDINATES: protein motif:HMM:NF008653.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEU5465877.1"
                     /translation="MASVTLVGVQRIYPGPPPRVAVDNLDLAVADGEFMVLVGPSGCG
                     KSTTLRMIAGLEPVDAGHLSIGDRLMNDVAPRDRDVAMVFQNYALYPHMTAYRNMAFN
                     LVLKRMSKEDIDNRVREAASTLRIEHLLERRPRQLSGGEQQRVALGRALVRNPAVFLF
                     DEPLSNLDAKLRVEMRREIARLHRQLGTTMVYVTHDQAEAMTLGNRIVVMHGGKVQQV
                     GKPLDVYRQPKNTFVAGFIGSPPMNLIDGRLTPDGARFVSEGGVFTLVPPAEWRAPLA
                     KSGGRAVVLGIRPEHVTLTGAGQAVQAHVDLVEPLGQEVLVYTTASSNAASCEITARL
                     QGGPLPVTGETAAMHLDPSGLVFFDAETRERIG"
     gene            4578..5921
                     /locus_tag="VFU75_03260"
     CDS             4578..5921
                     /locus_tag="VFU75_03260"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HEU5465878.1"
                     /translation="MSDSTRFPEDFLWGAATSAYQVEGSPLADGAGPSIWHRFSHSPG
                     LTAGGETGDVACDHYRRWSEDVALMADLGLNAYRFSISWSRVLPDGRGRVNRAGLDFY
                     SRLVDALLAKNIQPNVTLYHWDLPAALDDRGGWLNPDIAGWFADYARVCFKALGDRVK
                     MWATLNEPWVVVDGGYLHGALAPGHRNVFEAPRVAHHLLLSHGAGVQAYRAEGKGKIG
                     IVLNIEPKHPATQDPNDLAAVRRADAYMNRQYLEPVLGRPYPAELAEVYGPGWDSSVQ
                     QDIAKIAQPIDFLGINYYTRGVTRDDPSAHPVRTAPVRVPGATYTETGWEVYPAGLTE
                     TLLWVKERSGALPLYVTENGAAFPDPQIEDHSRVSYYREHLRAALEARKQGVDLRGYF
                     AWSLLDNFEWSLGYAKRFGIVHVDFATQKRTLKASARLYRDVIRSRGGVLAVMAD"
     gene            complement(5924..6748)
                     /locus_tag="VFU75_03265"
     CDS             complement(5924..6748)
                     /locus_tag="VFU75_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEU5465879.1"
                     /translation="MRRHRVASIALHVALGLAALGTLFPLLWMVSGSLMATGEANSFP
                     PRLFPAHPTLSHYAALFTRLNVGRYFLNSVIVAGGATVISLLLNSMAGYALAKLHFKG
                     RAQLFRVLVAGLVIPAQVAMLPLFLVLQAMGLVNTYVGAMIPAMATIFGIFLIRQYAM
                     GIPDDLLAAARMDGASEFRIYWSIVMPVIKPILATMALFTFLSTWNDFLWPLVALTDD
                     AKYTLPVALAGLAGEHVQDTELMLAGSVVTVVPVLLAFIALQRFYVEGITAGSVKQ"
     gene            complement(6745..7638)
                     /locus_tag="VFU75_03270"
     CDS             complement(6745..7638)
                     /locus_tag="VFU75_03270"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5465880.1"
                     /translation="MSRAPGERAQARAAWVFLAPALLLITTFFFLPVVTGLALSLTDF
                     DLYSIGHPDNIRMVGTANYRALAQAPMFWSALKNTLYFAFVGAPLSVLAALAAALLVN
                     SRLTKWKGFFRTAYFAPFVTTLVAVAIVWKYLYHPRFGLLDAALGAVGVHAIDWLGNP
                     KWAMPAIIVVAVWRSFGYNMLIFIAGLQRIPHELREAAELDGAGLWARLRHVTLPQLT
                     PTFVFVGVITAIGFFQLFAEPYVMTGGGPLNSTLSMVLYMYQEGFRWWRLGSAASIAF
                     VLFALVLVVTILQLLWRREET"
     gene            complement(7635..8912)
                     /locus_tag="VFU75_03275"
     CDS             complement(7635..8912)
                     /locus_tag="VFU75_03275"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEU5465881.1"
                     /translation="MAVCIAPIAMIFTACNARAPTDGVVLDFWGMGREGEVAQQMMPE
                     FEAEHPGVHVHVQQIPWTAAHEKLLTAYVGHVSPDVAQLGNTWVPEYVALGAIVPLDS
                     LIKRTPSITANDYFEGIWATNVLGDTVFGIPWYVDTRLLFYRKDLLAQAGFPSPPTTW
                     AQWIAALKAIKSRDEPGQYPIYIPTNEWPPPTILALESGSPVLKDGGRYGAFDDSAFK
                     TAFHFYLSFFQNGLAPKLGINEVGNIYQEFERGTYAMWITGPWNIGEFRRRTPAGFQD
                     KWATAPMPGPTGAASGVSLAGGSSLVIYKASRHPKEAWELIEYLSRPEQQLRFYHLTG
                     DLPARIETWRDTSLADDPAAHAFEEQLHRVVPTPQVPEWELIATRIFDYGEQAIRGGV
                     PADTALARLDTEVNNILERRRWLLDRPHRHEGR"
     gene            complement(9047..10477)
                     /locus_tag="VFU75_03280"
     CDS             complement(9047..10477)
                     /locus_tag="VFU75_03280"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEU5465882.1"
                     /translation="MIDAHHAGRRLTAICTVLPAAWALLGCATNHPEGPTPPDSVQSA
                     AFLDTLEERTFHYFWDLSNSTNGLTPDRSPSPSFSSIAAVGFALTAYPIGVERGYVTR
                     AEAAARTLATLRFFWTSTQDSSATATGYHGFYYHFLDMNSGARYQTVELSTIDTGLLL
                     AGVLTCQSYFSGADSTETAIRAVAESLYDRADWRWIQPRAPLVAMGWKPESGFLAYDW
                     KGYDEGMILYILALGSPTHAVDSTAWDAWTSGYQWGTFEGQTYLGFAPLFGHQYSHVW
                     IDFRGIQDGYMKGQGSDYFENSRRATLAQQAYAVANPGHWTGYAAGFWGLTASDGPLD
                     GTISSRQYHTYDARGASFTNIEDDGTVAPAAAGGSVPFAPEITIPTLYAMRHTYGAAL
                     FSTYGFFDAINPTFPTTATPSQGHVVSGIGWVDTDYLGIDQGPILAMVENYRTGLIWK
                     LMRGNAHIVTGLCRAGFTGGWLAGRC"
     gene            complement(10553..13603)
                     /locus_tag="VFU75_03285"
     CDS             complement(10553..13603)
                     /locus_tag="VFU75_03285"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU5465883.1"
                     /translation="MSRLAGLRHFGASLFGVALLVLAAAPPAATQTTTGTIRGYVRDS
                     SGAPFAGAEVEAKNPATGGVRTTTTNADGSYVLAGLLPTVYDLTVRHIGSAPSTRRVV
                     VQIGTTTTADFIPTAQAVEVAGVNVQAAAPAFDLKTSEVATNISTQQLQQLPTQSRNF
                     LDFAQLAPGVQVSEDRINAVSTRTVAAGGGSANQTNVFIDGASLKNDLTAGGIAGQDA
                     SRGNPFPQNAIQEYRVITQNFKAEYQQAGTAVITAATKSGTNVWQGSAQFQYSDQGLF
                     ALDSFQIAQKQTKPDFSRVLPSISIGGPLMRDKLFFFGSYEGNYQNRSNLINFSPIGG
                     FSALDSVNLKQYNGLFTSPFRETLLFGKLDYNAGEHSTAQLSWTQRIEHDIRDFGTDN
                     AGNGTSYSNAVNLKNTDGIGNLKYTYSTGPWLSETQANYENLERNPGPNFPGSFARQY
                     FYFASGAPSAPLLNNMIGSNLSIQDYTQQRFALRSDVTYTGFHGMGDHVFKAGVSLAH
                     LKYDIIKENNITPQFLYTDSVQTNCWCRTRDTTSGFHAFDFTQPYQLSYGTGLPGLNT
                     TNNEIGAYLQDDWSPTTRLTLNLGIRWDFESNMINTGYVTPQSVVDTLTRYNDSLPTP
                     LDLNRYISNGHNRSPFYGAFQPRVGFSYAMDKNSKTTLFGGAGIYYDRSIFDFSIDEA
                     QRLTYPVYTVRFAAPGQAPTAGQAAWNNSYLTTDTTVLNALVHSSGLPEPYLLDNNMT
                     PPKYTQWSLGIRHMMGSWLGTVTYEGQRGVNLFTYNWANIGLKPDGTCCVSFNIGAHG
                     FNNFIYSTEDGKTWYDALTFEFQRPFQRGASGLGWSAGVSYTFATRYVAGVDNLNDIG
                     SSFPGGFPNAKSIPKHPSDGGQDERQRIVGNWVVDMPWVSGLQFSGLLTLGSGPTTDA
                     GCPPRFGCVPGKTFSYGGFTPPPANGFIPGGWAYQRVDLRLQKNFPEFAGQNLGVTFD
                     VYNVFNTQNLGCWGVGYSAPNLSYNAGCTVSDPRRVQLGALYSF"
BASE COUNT         2247 a         4265 c         4679 g         2452 t
ORIGIN      
        1 tggtcttgcc cggagacgcg gacaccttcg caattcgctg gccgacgatc gcgttcagga
       61 gactcgactt tccgacgttg gaccgtccga gaaaggcgat ttcggggtgg ggaggatcga
      121 gtgtgacatc ggcccggggg aacgagccga cgaaccgcgg gtcggtcagg cttctttctt
      181 ctggcgcggc cgctcggtga ccaggagggg ctgccggccg gcggtgacgc attcggcggt
      241 gatggcgact tcgcgcacgt cgtcacggcc cggcaggtcg aacatgattt ccgtcatcat
      301 ctcttcgagg atggcgcgga gcccgcgggc accggtgccg cgcttgatcg ccttctgggc
      361 gatggccttc aaggcgtcct tgtcgatggt aaggcggacg tcttccagct cgaagagctt
      421 ggcgtactgc ttggtgagcg cgttcttggg ctcgaccagg atccggacca gcgactcctc
      481 gtccagggcg tcgaggggga cggtgaccgg caggcggccc accagctcgg ggatcaggcc
      541 gtagcggagc aggtcgtccg gttccacgtc cacgaagggg ttcttcttcc gcttgtcggc
      601 ctcacccttg gcgacctctt ccttgctgaa tccgatctgc cggcggccgg tgcgggactc
      661 gatgatcttc tcgaggccgt cgaaggcgcc tccgcagatg aacaggatgt ccttggtgtt
      721 gacctggatg tattcctgct gcggatgctt gcgtccgcct tgcggcggca ccgaggcgac
      781 cgtgccctcg aggatcttca gcagggcctg ctgcacgcct tcacccgaga cgtcccgcgt
      841 gatgctcggg ttctcggact tccgggcgat cttgtcgatc tcgtcgatgt acacgatgcc
      901 ccgctcgcac tcggtgacgt tgaaatcgcc ggcctggagg aggcgaacca ggatgttctc
      961 gacgtcctcg cccacgtacc ccgcctcggt gagcgtggtg gcgtcggcga tggtgaaggg
     1021 aacgtccagg atgcgggcca gggtctgcgc gagcagggtc ttgcccacgc cggtggggcc
     1081 gatgaggagg atgttgctct tgtcgagctc cacctcggac tcacgcccgt tggagtggga
     1141 attgatgcgc ttgtagtggt tgtagacggc gacggcgagg gtcttcttgg ccttctcctg
     1201 cccgatcacg tactggtcca gcacgtcctt gatttcctgc ggcgtgggga cctgcgtgat
     1261 cgcttcggcg acttcccgct cttcgtcctc cgccagaatt tcgttacaaa gcgcgatgca
     1321 ctcgttgcag atgtacaccg acggcccgga gatgaatttc cggaccgagt ccttcgactt
     1381 gccgcagaag gagcaacgga ggtgcttatc gtgtgacata tcggctagta tgggaaaagg
     1441 gccctatcgg cgcaagggct caggcctttt tcccgtccaa atccttgagt tgtgcgatga
     1501 cgttgtcgat gaggccgtac tccttggcct cttccgcgga catgaagcgg tcccggtcca
     1561 tgtccttctc gatctgctcc agcggcttct tggtgtgctt cgagtacagc tcgttgagct
     1621 tgccacgcag gtagatgatt tcgcgggcct ggatttcgat gtccgccgcg gtgccggacg
     1681 cgccgcccga aggctggtgc atcatgatcc gcgcatgggg cagcgcggag cgcttgcccg
     1741 tggtgcccgc ggtgagcagg aacgagccca tggaggccgc catgcccatg cagatggtgt
     1801 tcacgggggc gcgcaggaac tgcatcgtgt cgtaaatggc catgccagac gataccagtc
     1861 cgcccgggga attgatgtag agatagatgt cccgttcggg gttgtccgcg tcgagaaaca
     1921 acagctgggc gatgatgatg ttcgccacgg tgtcgtcgat cgcggtgccg aggaacacga
     1981 tgcggtccat caggaggcgc gaaaagatgt cgtaggtgcg ctcgccccgg ctggaacgct
     2041 cgatgatgta cggcggatag atcgtgcccg aggtcatgca ttctccacgg tggactgcga
     2101 cagaaggaag gtaaacacct tctcctcggt aaggcctcgc tcgagatcac gaagccgctt
     2161 ggccttctcc agggacgcgt acagctcgcg ggccggctgg ccccgcttcg ccgcgagctc
     2221 ttcgatcttg gcgtccagct ccgcttcggt ggcggccaga gtgttcgcct cgactacata
     2281 gtcgaggatc aggtcccggc gcacctgctg ttcggccacc gaacgcattt cggtcgagaa
     2341 ctgttcccag cggtcttcgg ggatgccgta cgcctgggcg tagacggcca gggcccgatt
     2401 cacgagcgga gcgggggcca ccacggagtt cgccgcgacg atctgttcga tcagcgccgc
     2461 ccgcacgttg gcgtcggcct gccggtcggc ttccttctcc agatcctcac ggaccgcctt
     2521 ccgcagcgcc tcgaccgagt cgaagtcacc gacctcgcgc gcgaacgcgt cgtccagcgc
     2581 cggcagctcc tgccgcttca cctcggtgag ggtgatccgc acctcgcgcg actggccccg
     2641 cttcgcttcc tcggggaagt cggcggggaa cttgaccgtc gcgtccaccg tctcgccggg
     2701 gaggagggac atcacttttt cctcgacgtc cgggatggca cgcccctcgc cgagtacgaa
     2761 ctgatacggc tgggtgtcct tgatctcctc tccctcgcgc tgggcgaggg tgatgtgcac
     2821 cagatccttc ggcttgggct tctcgccggc cagcgggatc cagggcgcct tctggtcgcg
     2881 caggtcattg atctgcgcct cgacctggtc ggccgagacc ttcgacaccg ttcgcttgag
     2941 cgtgaacccg ccgagtcgct cgagcttgag ctcgggcttc acttcgacgt gaaactcgaa
     3001 ggtgaccggc gtgccctgct cccacttcag attgtggatg tggggatcgg cgatgggctt
     3061 gagcttctcc tggtccaccg ccgccttcca gctctcctgg agaatctcct ggagggcctg
     3121 ctgacggatg tcctcggcga aacgcttttt cacgacggcc gacggggctt tgcccttccg
     3181 gaatcccggc aaccgggcgc gcgactgata ggcgcgcgtg gcgcgctcct cggcggcccg
     3241 gacgttctcc tccgggacgc tcaccgccag ggacgccgcc ccgggctcgg aactggtttt
     3301 ggtgatttcg atggtcatgt gggcctagaa tctaaccatg ggggggaggg ataacgggag
     3361 ccgagagccg ggagccgtct gagtcgggaa gagacggact ccatgaggct tacggggtct
     3421 ccaactcctg gactgcgacg gctcccggct cccggcgcct tatccgatcc gctccctcgt
     3481 ctcggcatca aagaacacca ggccgctggg atccaggtgc atggctgcgg tctctccggt
     3541 caccggtaag ggaccgccct ggaggcgcgc ggtgatctcg caggacgccg cgttggagct
     3601 ggcggtggtg tagaccagca cctcctgccc cagcggttcc acgaggtcca catgggcctg
     3661 gaccgcctgg ccggctccgg tcagcgtgac gtgttccggc cgtatgccga ggaccaccgc
     3721 gcgtcccccg gatttcgcaa ggggcgcccg ccactcagcg ggaggcacca gcgtgaacac
     3781 gcctccctcc gatacgaagc gcgcgccgtc gggcgtcagg cgtccgtcga tcaggttcat
     3841 gggcggactg ccgatgaacc cggcgacgaa cgtgttcttc ggctggcggt agacgtcgag
     3901 cggttttccg acctgctgca ccttcccgcc gtgcatcacc acgatccggt tgcccagggt
     3961 catcgcttcg gcctggtcgt gcgtgacgta caccatcgtg gtgccaagct gccggtggag
     4021 ccgggcgatt tcccggcgca tttcgacgcg gagcttggcg tccaggttgg agaggggttc
     4081 gtcgaagaga aagaccgcgg gattccgcac cagcgcgcgg ccgagggcga cgcgctgctg
     4141 ctcgccgccg gagagctggc gcggccggcg ctcgagcagg tgctcgattc gcagcgtgga
     4201 cgcggcctct ctgacgcggt tgtcgatgtc ttctttcgac atccgcttga gcaccagatt
     4261 gaacgccatg ttccggtacg ccgtcatgtg cggatagagc gcgtaattct ggaagaccat
     4321 cgccacgtcc cggtcccgcg gtgcgacgtc gttcatcagg cgatcgccta tggagaggtg
     4381 acccgcgtcc acgggctcca gaccggcgat catccggagg gtagtgctct tgccgcatcc
     4441 cgacggcccc accagcacca tgaactcgcc gtccgcgacg gcgagatcga ggttgtccac
     4501 cgcgacgcgc ggcggggggc ctgggtatat tctctgtacg ccgaccaggg ttacgctcgc
     4561 catgcccgcc gtctccgatg agtgattcca cacgcttccc cgaggatttc ctctggggtg
     4621 ccgcgacatc cgcctaccaa gtggaaggat cgcctctcgc cgatggggct ggtccgtcca
     4681 tctggcaccg tttctctcat agcccgggcc tgaccgccgg tggggagacc ggcgacgtcg
     4741 cgtgcgacca ctatcgccgg tggtcggagg acgtggcgct catggccgac ctgggcctca
     4801 atgcctaccg cttttcgatt tcgtggagcc gcgtccttcc ggatggccgg ggccgcgtga
     4861 accgggcggg gctcgatttc tacagccggc tggtggacgc gctgctcgcg aagaacatcc
     4921 agccgaacgt caccctctat cactgggacc tccccgccgc cctcgacgac cgcggcggct
     4981 ggctcaaccc cgacatcgcc ggctggttcg cggactacgc gcgggtctgc ttcaaggcac
     5041 tgggtgaccg cgtgaagatg tgggccaccc tgaacgaacc ctgggtggtc gtggatggcg
     5101 gctatctgca cggcgccctc gcccccggcc atcggaacgt cttcgaggcc ccgcgcgtcg
     5161 cccatcacct tctgctgtcc cacggtgccg gcgtgcaggc ctaccgggcc gagggcaagg
     5221 ggaaaatcgg gatcgtcctg aacatcgagc cgaaacatcc ggcgactcag gaccccaatg
     5281 acctggcggc cgtccgtcgc gcggatgcct acatgaaccg gcagtacctg gaaccggtgc
     5341 tcggccgccc gtaccccgcc gagctcgccg aggtgtatgg gccggggtgg gacagctcgg
     5401 tgcagcagga catcgcgaag atcgctcagc ccatcgactt tctcggcatc aactactaca
     5461 cgcgcggagt gacgcgcgac gatccctcgg cccacccggt tcgaaccgcg cccgtccgcg
     5521 taccgggtgc gacgtacacc gagaccggat gggaggtcta tcccgcgggc ctcacggaga
     5581 ccctgctctg ggtgaaggag cggtcgggcg cccttcctct ctatgtcacc gaaaatggtg
     5641 ccgcgttccc cgatcctcag atcgaggacc actcccgtgt ctcctactac cgcgagcatc
     5701 tccgggccgc gctcgaggcc cggaagcagg gcgtcgacct ccgcggatac ttcgcgtggt
     5761 ctctgctcga caacttcgag tggagcctgg gctacgcaaa gcgcttcggc atcgtgcacg
     5821 tcgatttcgc gacgcagaag cgcacgctca aggcgagtgc ccgcctctat cgtgacgtga
     5881 tcaggtcccg cggcggcgtt ttggccgtca tggcggactg aggtcactgc ttcacgctcc
     5941 ccgcggtgat gccctcgacg tagaagcgct gcagggcgat gaaggcgagc aggaccggta
     6001 ctaccgtgac cactgacccc gccagcatca gctccgtgtc ctgcacgtgt tcaccggcga
     6061 gcccggcgag ggccacggga agcgtgtact tcgcgtcgtc ggtgagggcg acgagaggcc
     6121 agaggaagtc gttccaggtg gagaggaagg tgaagagggc catcgtcgcg aggatcggct
     6181 tgatcaccgg catcacgatg gaccagtaga tccggaactc gctcgccccg tccatccgcg
     6241 ccgccgcgag gaggtcgtcg ggaattccca tcgcgtactg gcggatgagg aagattccga
     6301 aaatcgtcgc catcgccgga atcatcgcgc cgacgtaggt gttcacgagt cccatggcct
     6361 ggagcaccag aaacaggggc agcatggcga cctgggccgg aatcaccaga ccggcgacga
     6421 gcacccgaaa gagctgggcc cttcccttga agtggagctt ggcgagcgcg taccccgcca
     6481 tggagttcag gagcagcgag atcaccgtcg cgccccccgc cacgatcacg ctattcagga
     6541 aataccggcc cacgttgagc cgcgtgaaca acgcggcgta gtgggagagc gtggggtggg
     6601 cggggaagag gcgcggcggg aagctgttgg cctcaccggt cgccatgagg gatcccgaga
     6661 ccatccagag cagcgggaac agggtgccca gggccgcgag ccccagcgcc acgtggaggg
     6721 cgatcgacgc cacgcggtgt ctcctcacgt ctcctcccgg cgccagagga gctggaggat
     6781 ggtcaccacg agcacgaggg cgaacagcac gaacgcgatg gacgccgccg atcccagccg
     6841 ccaccagcgg aagccttcct gatacatgta gaggaccatc gacagcgtgc tgttgagagg
     6901 cccacctccc gtcatcacgt acggctcggc gaacagctgg aagaacccga tcgcggtaat
     6961 cacgccgacg aacacgaagg tgggtgtcag ctggggcagg gtcacgtgcc gcagccgggc
     7021 ccacagtccc gcgccatcca gctcggcggc ctcgcgcagc tcgtgcggga tccgctgcag
     7081 ccccgcgatg aagatcagca tgttgtaacc gaagctccgc cacaccgcga ccacgatgat
     7141 cgcgggcatc gcccacttcg gattgccgag ccagtcgatg gcatgaaccc cgactgcgcc
     7201 cagggcggcg tcgaggagcc cgaagcgcgg gtgatagagg tatttccaga cgatggccac
     7261 ggccacgagc gtcgtgacga agggcgcgaa atacgcggtg cggaagaatc ccttccattt
     7321 ggtgagccgc gagttcacga gcagggcggc cgcgagcgcg gcgagcaccg agaggggggc
     7381 gccgacgaac gcgaagtaca aggtgttctt gagcgccgac cagaacatcg gcgcctgggc
     7441 gagggcgcgg taattcgcgg tgcccaccat ccggatgttg tccgggtgcc cgatggagta
     7501 gaggtcgaag tcggtgagcg agagggccag gccggtcacg accggaagga aaaagaaggt
     7561 ggtgatgagg agcagggccg gcgcgaggaa cacccacgcg gcgcgcgcct gcgcccgttc
     7621 ccccggcgcg cgactcaccg gccctcgtgc cggtggggcc ggtccagcag ccagcgccgc
     7681 cgctccagaa tgttgttgac ttcggtatcc aggcgggcga gcgccgtatc ggcgggtaca
     7741 ccgccccgga tcgcctgctc gccatagtcg aaaatgcgcg tcgcgatgag ctcccactcg
     7801 gggacctgag gggtcgggac cacccggtgc agctgctctt cgaacgcgtg cgcggccggg
     7861 tcgtcggcga gcgacgtgtc gcgccaggtc tcgatgcgcg cgggcaggtc tccggtgaga
     7921 tgatagaacc ggagctgctg ctccgggcgc gacaagtact cgatcagctc ccacgcttcc
     7981 ttggggtgcc gggaggcctt gtagatcacg aggctcgagc cgcccgccaa cgagacgcct
     8041 gaggccgcac cggtcggtcc gggcatgggc gcggtcgccc acttgtcctg gaagcccgcg
     8101 ggggtgcggc ggcggaactc gccgatgttc cacggccccg tgatccacat cgcgtaggtg
     8161 ccgcgctcga actcctggta gatgttgccg acttcgttga tgccgagctt cggtgccagc
     8221 ccgttctgga agaagctgag gtagaagtgg aaggccgtct tgaacgccga gtcatcgaag
     8281 gcaccgtatc gcccgccgtc cttcagaacc ggtgacccgg attccagcgc gagtatggtg
     8341 ggtgggggcc attcgttggt cgggatgtaa atggggtact gcccgggttc gtcgcgcgac
     8401 ttgatggcct tgagggcggc aatccactgg gcccatgtcg tgggcggcga cgggaatccc
     8461 gcctgtgcca gcaggtcctt ccggtagaac aggaggcggg tgtccacgta ccacggaatc
     8521 ccgaacacgg tgtcacccag gacgttggtc gcccagatgc cctcgaagta gtcgttcgcg
     8581 gtgatactcg gtgtacgctt gatcagggag tcgaggggga cgatggcgcc gagcgcgacg
     8641 tattcgggga cccaggtgtt gccgagctgg gcgacgtcgg gagaaacgtg gccgacgtag
     8701 gcggtgagca gcttctcatg cgccgcggtc cacgggatct gctgcacgtg cacgtgcacg
     8761 ccgggatgct cggcctcgaa ctccggcatc atctgttggg cgacttcacc ctcacgcccc
     8821 atcccccaga agtcgagcac gaccccatcg gtgggggcgc gggcgttgca ggcggtgaag
     8881 atcatcgcga tcggcgcgat gcagactgcc atccaccctc gtcgaaccca ggctgaaagc
     8941 ctgggcccga cacgacgcgg cgcttcaagc gcaatgcctt gtcgggccca ccctttcagg
     9001 gtggggacga tcggggccgg gagcggcttg aaggccacgt gatggcctag caccgccccg
     9061 ccagccatcc gccggtgaat ccggcccggc acagtccggt gacgatatgg gcgttgccgc
     9121 gcatgagttt ccagatcagg cctgtccggt agttctcgac catcgcgagg atggggccct
     9181 ggtcgatgcc gaggtagtcg gtgtcgaccc agccgatgcc ggagaccacg tgtccctggc
     9241 tgggggtggc ggtggtgggg aaggtcggat tgatcgcgtc aaagaacccg taggtcgaga
     9301 agagtgccgc gccgtacgtg tggcgcatcg cgtacaacgt cgggatcgtg atttccgggg
     9361 cgaacggcac ggatccaccg gcggcggccg gcgcgacggt gccatcatcc tcgatgttcg
     9421 tgaaggacgc gcctcgcgcg tcgtacgtgt ggtattgccg ggaggagatg gtcccgtcca
     9481 ggggtccgtc ggaggcagtg agtccccaga agccggcggc gtatccggtc cagtgtccgg
     9541 ggttggcgac ggcgtacgcc tgctgggcca gcgtcgcgcg ccgcgagttc tcgaagtaat
     9601 cgcttccctg gcccttcatg tagccgtcct ggatgccacg gaagtcgatc cacacgtgcg
     9661 agtactggtg cccgaagagg ggggcgaagc cgaggtaggt ctgcccttcg aacgtgcccc
     9721 actgatagcc gctcgtccac gcgtcccagg cggtcgagtc gaccgcgtgc gtcggcgagc
     9781 cgagggcgag gatgtagagg atcatcccct cgtcgtagcc cttccagtcg taggccagaa
     9841 acccgctctc gggtttccac cccatggcga cgagaggagc gcgaggctgg atccaccgcc
     9901 aatcggcgcg gtcatagagc gactccgcga ccgcgcggat ggccgtttcg gtcgagtcgg
     9961 cgccggagaa gtacgactgg caggtgagga cgccggccag gaggagcccg gtgtcgatcg
    10021 tcgagagctc gacggtctgg tacctcgcgc ccgagttcat gtcgaggaag tggtaataga
    10081 acccgtgata tccagtggcc gtcgccgagg agtcctgcgt ggaagtccag aagaatcgca
    10141 gcgtcgccag ggtccgggcc gcggcttccg cgcgggtgac gtagccgcgt tcgaccccga
    10201 tgggataggc ggtgagggcg aagccgacgg cggcgatgct ggagaacgag ggacttggag
    10261 atcggtccgg cgtgagtccg ttggtcgagt tcgagaggtc ccagaagtaa tggaaggtgc
    10321 gctcttcgag agtgtccaga aatgcggcgg actggacgga atcaggcggg gtggggcctt
    10381 ccgggtggtt cgtcgcgcag ccgaggaggg cccacgctgc cgggaggacg gtgcagatgg
    10441 ccgtcaaccg cctcccggcg tggtgtgcgt caatcatcag gtgagaatac ccccgcccca
    10501 cccggagggc caccggcacc cgaatgccgg tgaccgctgc cgagggagtg gttcagaagg
    10561 agtacagggc gccgagctgt acgcgtcggg gatcggagac cgtgcatccc gcgttgtagc
    10621 tcaagttggg agcggaatat ccgacgcccc agcagccgag gttttgcgtg ttgaacacgt
    10681 tgtagacgtc gaaggtgacg ccaaggttct gaccggcgaa ctccgggaaa ttcttctgga
    10741 gccggagatc gacccgttga taggcccacc cgccggggat gaagccgttc gccggcggcg
    10801 gcgtgaaccc cccgtacgag aaggtctttc caggcacgca cccgaaccgg ggcgggcagc
    10861 cggcgtccgt cgtcggacca ctcccgagcg tcaggagccc gctgaactgc aggcccgaca
    10921 cccagggcat gtcgacaacc cagttgccga cgatccgctg gcgctcgtcc tgcccgccgt
    10981 cggacggatg cttcgggatg gacttcgcgt tcgggaaacc gcccgggaat gacgacccga
    11041 tgtcgttcag attgtcgaca ccggcaacgt accgggtagc gaacgtgtag gagacgccgg
    11101 cgctccaccc caaaccactc gccccgcgct gaaacgggcg ttggaactcg aaggtgaggg
    11161 cgtcgtacca ggtttttccg tcctcagtcg agtagatgaa gttgttgaac ccgtgcgctc
    11221 cgatgttgaa gcttacgcag caggtgccgt cgggcttgag cccgatgttc gcccagttgt
    11281 aggtgaacag gttgacgccg cgctgcccct cgtaggtcac ggtgccgagc cacgagccca
    11341 tcatgtgccg tatgccgagg ctccactggg tgtacttcgg cggcgtcatg ttgttgtcga
    11401 gcaggtacgg ctccggcagc ccggagctgt gcacgagcgc gttgagcacc gtggtgtcgg
    11461 tcgtgaggta cgagttgttc caggccgcct ggcccgccgt aggggcctgc ccgggcgccg
    11521 cgaaacgaac ggtatagacg gggtaggtga ggcgctgcgc ttcgtcgatg gagaagtcga
    11581 agatcgagcg gtcgtagtag atcccggcac ccccgaagag cgtggtcttc gagttcttgt
    11641 ccatcgcata ggagaacccg acccgcggct ggaaagcgcc gtagaacggg gaccggttgt
    11701 ggccgttcga gatgtagcgg ttgaggtcga ggggcgtcgg cagggaatcg ttgtaccggg
    11761 tcagcgtgtc cacgacgctt tgcggggtca cgtacccggt gttgatcatg ttggactcga
    11821 aatcccagcg gatccccaga ttcagcgtca gtcgcgtggt gggactccag tcgtcctgga
    11881 ggtacgcacc gatctcgttg ttggtcgtgt tgaggcccgg aagtcccgtg ccgtacgaca
    11941 gctggtaggg ctgggtgaaa tcgaaggcgt ggaagcccga cgtcgtgtcc cgggtgcggc
    12001 accagcagtt ggtctggacg gaatccgtat agaggaattg cggggtgatg ttgttttcct
    12061 tgatgatgtc gtacttgagg tgcgccaggc tcaccccggc cttgaagacg tgatcgccca
    12121 tcccgtggaa accggtgtac gtcacgtcac tccgcagcgc gaaccgctgc tgcgtgtagt
    12181 cctgaatgct caggttgctc ccgatcatgt tgttcagcag cggcgcgctg ggtgcccctg
    12241 acgcgaagta gaagtactgg cgggcgaagg accccggaaa attcggaccc gggttgcgtt
    12301 ccaggttctc gtagttcgcc tgcgtctcgc tgagccacgg gccggtcgaa taggtgtact
    12361 tcaggtttcc gataccgtcg gtgttcttga ggttcacggc gttgctgtag gaggtcccgt
    12421 tacccgcgtt gtcggtcccg aagtcccgaa tgtcatgttc gattcgctga gtccaactca
    12481 gctgagccgt ggaatgctcg ccggcattgt agtcgagctt gccgaacagg agtgtttccc
    12541 ggaacggtga ggtgaacagg ccgttgtact gcttgaggtt cacggaatcg agggccgaga
    12601 acccgccgat gggactgaag ttgatcaggt tcgaccggtt ctgataattg ccctcgtagg
    12661 acccgaagaa gaacagcttg tcccgcatga ggggtccccc tatgctgatc gagggaagca
    12721 cccggctgaa gtccggcttc gtctgcttct gagcgatctg gaaggaatca agcgcgaaga
    12781 gcccctggtc gctgtactga aactgcgccg agccctgcca cacgttggtg cccgacttcg
    12841 tcgccgccgt gatcaccgcg gtgccggcct gctgatactc cgctttgaaa ttttgcgtga
    12901 tcacgcggta ttcctgaatc gcgttctgcg ggaacgggtt cccgcggctc gcgtcctgac
    12961 ctgcgatccc gcccgccgtg aggtcgttct tcaggctggc gccgtcaatg aagacgttgg
    13021 tctggttcgc gcttccgccg cctgccgcga ccgtgcgcgt cgagaccgcg ttgatccggt
    13081 cttccgagac ctggacgccg ggagccagtt gggcgaagtc caggaagttc cggctctgcg
    13141 tcggcagctg ttgcagctgc tgggtggaaa tgttggtggc cacctccgac gtcttgaggt
    13201 cgaaggccgg tgccgcggcc tggacgttga cgcccgcgac ctccaccgcc tgggcggtcg
    13261 ggatgaaatc cgcggtcgtc gtcgtgccga tctgtacgac gacccggcgg gtggaaggag
    13321 ccgagccgat gtgacgtacc gtcaggtcgt agaccgtcgg cagcaggccg gcgagcacat
    13381 aggacccatc cgcgttggtc gtcgtggtcc ggaccccacc cgtcgcgggg ttcttggcct
    13441 cgacctcggc gccggcgaag ggcgcgccgc tcgagtcgcg cacgtacccc cggatcgttc
    13501 cagtcgtggt ctgggtcgcg gcggggggcg cggccgcgag gaccaggagc gccactccga
    13561 agagcgacgc gccgaagtgt ctcaagcctg cgagacggga catagcaact ccctcctgtt
    13621 acggtgactc ggttgaagca cgg
//
LOCUS       DASUBP010000083        10184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_70264, whole genome shotgun sequence.
ACCESSION   DASUBP010000083 DASUBP010000000
VERSION     DASUBP010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 10184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10184
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70264"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1658)
                     /locus_tag="VFU75_03290"
     CDS             complement(<1..1658)
                     /locus_tag="VFU75_03290"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5465884.1"
                     /translation="MPDLRPQLEAALSGRFRIERELGGGGMSRVYLAEEVGLARKVVV
                     KVLAPELSAGVNADRFRRETQLAASLQHPHIVPLLTAGTAGDLLYFTMPFVDGESLRA
                     RVKREGALPIRDAQRILHDVADALAYAHARGVVHRDIKPDNVLLSGNHALVADFGIAK
                     AISSAGGSSGLTSVGVAIGTPAYMAPEQAAGDPTVDHRADIYSFGAMAYEALSGHQLF
                     EGMSPQQTLAAHLTEPPEDIGKRRPQVPQELADLVMACLEKNAADRPQTAAEVLRVLE
                     GTATLTSGGTKHSTRTRTGTVAALKPRTRWIVTAVAALAGIALVWGIVGRSGGSMLDD
                     DKVAVAPFDVLDPSLQLWREGLVDILARSLDGAGPLRTVSPTVIVKRWSGRADPIAAR
                     ALGENTGARIVVYGALLTSGTDSVRLSANVLDVSTGNQLGAVDLRDHKDRIDRLADSV
                     AVHVLQAIGGTRIRGLGLARTTGIGSASLPAVRAFLTGEQYLRRSEWDSALVAYQQAF
                     GIDSNFTLAYAHAGLAAGWGRSGSDSLTTRYQLKAGSLNHALAPR"
     gene            complement(1677..4913)
                     /locus_tag="VFU75_03295"
     CDS             complement(1677..4913)
                     /locus_tag="VFU75_03295"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU5465885.1"
                     /translation="MQRLLAAALAFAALPGPSPVSVFHTRVADAPGLGYYRFPALHGD
                     SLVFTAEGDLWMVDAAGGVARRLTSGQGEETNATISPDGKTIAFVGSYEGPAEIYTVP
                     MSGGVPIRRTWGGGLPVGWTPDGKLLMMSGALSTLPEPRLFTLDLSTGVRSPIPLVQA
                     SDGTYDRAGGTLYFTRFAFQGSHTKRYKGGTAQSLWKLPAGASEATPLTGDYTGTSKT
                     PMWWAGRVYFVSDRDGSMNLWSMDPEGHDLKQLTHHQGWDIQSPALANGKIVYQLGAD
                     LWLYDIATAQGHLIPVTLTSDFDQLRVRWVTKPMDYVTATHVSPNGDRVVLTARGQVF
                     VAPVTQGRLVEASRHQGVRYRSARFAPDGKSIMVLSDESGETDWWRLPANGVGGAEPV
                     TNDAKVLRWDGVTSPDGKYLAYTDKNQTLWLVDLSAKKTTKIEESPDGGYDDLTWSPD
                     SRWLAYTRPGTTFSTIVLYSVVTGKATPVTTPRADSYSPGWSADGKWLYFLSDRNFQS
                     LIGGPWGPRAPEPFFDKQAKIYLVSLVKGLRSPFQPKDETPLPPPDTGKKDVSVTVDF
                     DGITDRLLEVPVPAGNYANLSVGSDRLFYLSFGPASRPPRALMALEISNQSPKPKTVA
                     DDMLQYELTADRKKLMIQKDGDLYVVDASSAPATLDAKGQVQLAGWSFPIDPREEFAE
                     MYREAWRLERDYFYDRHMNGVDWPAMRSKYAPLAARVTSRGELSDVLAQMVSELSALH
                     IFVYGGDFRQGDDQIQLGSLGATYERDEADGGWRITHIYKSDPDFPVQRSPLAMPALG
                     IAEGDVIQMVNGVPALSVPNFDALLENQTGKEVLLRVKAPGTTAGRDVVVTPMSGGAA
                     AGLRYSEWEYTRRVAVDAASHDSIGYVHLRAMTQNDIAQFERDFYPNLDRQGLIIDVR
                     HNNGGNIDSWVLEKLMRKAWMYWQGRIGKPYWNMQWAFRGHVVVLCDQRTASDGEAFS
                     EGFRRLGLGKVIGTRTWGGEIWLSSSNTLEDHGIATAAENGVYGPEGAWLIEGHGVDP
                     DSVVDDLPGATYRGQDAQLDAAIAYLRAEMKAHPTPVPPPPAYPDKSH"
     gene            5045..6061
                     /gene="coxB"
                     /locus_tag="VFU75_03300"
     CDS             5045..6061
                     /gene="coxB"
                     /locus_tag="VFU75_03300"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HEU5465886.1"
                     /translation="MHRHYRLIQRCIPGLLLLAVVALGACAGPFPQTTLHPKSDLGFA
                     IDHLFTGIFGWAVGVFVVVEGLLVYALFRFRAKPGAAAPHPTHGHTALEIAWTLAPVL
                     ILVMVAVPTIRTIFATQAPAPAGSLKIEVIGHQWWWEYRYPDQGVVTANELHLPVGRT
                     IALDLTSNDVIHSFWIPAIAAKRDVVPGHVNHIVFKADTVGEYMGQCAEFCGDSHANM
                     RLRAFVETDSAFNAWVANQKQPPVEPQPNTLQATGKNAFAHGACIACHTIQGVSAGMI
                     GPNLTHLASRTTIAGATYPDDSLDLALWVNHAPQRKPGSVMMDMNLQPANLQAIVAYL
                     MSLK"
     gene            6058..7854
                     /gene="ctaD"
                     /locus_tag="VFU75_03305"
     CDS             6058..7854
                     /gene="ctaD"
                     /locus_tag="VFU75_03305"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HEU5465887.1"
                     /translation="MTEPTGLKSWITTVDHKRIGKLYFWTAFCFFLVGGIEALLIRSQ
                     LAQPNGHIVDARTYNELFTMHGTTMIFLALMPLSVAFFNFIVPLQIGARDVAFPRLNA
                     FSYWVFLLGGIFMNVSWFVGPLFHAGRLSVAPNGGWFGYANLTERQYSIGPNIDFWLL
                     GLQILGVSSLAGGFNFVITILNLRAPGMKMMRLPVFTWMTLVTSFLLITAFPVITVAL
                     AFLMFDRFFGTNFYQLSAGGDPVLWQHLFWIFGHPEVYILILPAMGVVSEVLPTFSRK
                     PLFGYTVVVYSGILIAFMGWGVWSHHMFSVGLGPIADAVFSSTTMLIAIPTGVKIFNW
                     IATLWGGQIRFDTPLLFAVGFIAMFIIGGLSGVTHASPPADLQQTDTYYVVAHIHYVL
                     FGGTVLGLFAGMYYWWPKMTGRLLSETLGKIHFWGMLIGMNIAFFPMHFIGLLGMPRR
                     VYTYSPELGVSTMNLVSTIGAFLIAVSMLVFIWNVIRTRIKGKIAGDNPWQGATLEWA
                     TSSPPAVYNFAQIPTVHSRLPLWNEPESVAQRAPSGHIHVPDGSWWPLVSAAGLPLLA
                     TSLISGQLWLAFVAAAVLLVGIFKWGFEPFEV"
     gene            7855..8466
                     /locus_tag="VFU75_03310"
     CDS             7855..8466
                     /locus_tag="VFU75_03310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HEU5465888.1"
                     /translation="MSHAAATHTEGHHTSMGLDSRKMAFWAFIGSECLLFATLISTYL
                     IYSHRSIQGPYPQQILNIPFTSFSTFDLLMSSLWMVLALAAVQRNDMKWAKVWLMGTV
                     IGGLIFLLGQVWEFSHFVEHGLTLQRNLFGTTFFVLTGTHGAHVFVGVLWLFTLWVRA
                     MQGKLTSKNAMTVEITGLYWHFVDVIWIIIFTVVYLIQPVYHV"
     gene            8459..8764
                     /locus_tag="VFU75_03315"
     CDS             8459..8764
                     /locus_tag="VFU75_03315"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HEU5465889.1"
                     /translation="MSETHASEHPHPTAGLYLRVATVLFILTVLEVGVFYVPTFHPIL
                     VPALLILSAIKFCLVVMFYMHLKMDNRFFTMIFGGPLLLAFGMAMALMFLFKVFVLK"
     gene            8761..9609
                     /locus_tag="VFU75_03320"
     CDS             8761..9609
                     /locus_tag="VFU75_03320"
                     /inference="COORDINATES: protein motif:HMM:NF021214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HEU5465890.1"
                     /translation="MTPAPWDHWDIHPSVVIGLALFGGLYLFWGGTKASIWRQVSFWA
                     SVLVLFFSLNGPLHDLSDTYLFSAHMLQHLILTLAFPPLLLFGTPAWVVRPLLKPRWV
                     MALARTITKPLPAGIIFSTTIIFWHFPRFYELAMRHHNLHIVQHLFFIAASVVMWWPV
                     LSPVPELPRTSYPLQMVYLFVLGLPMSLVGAMITLADTPVYRFYVEAPLRVWGLSPLA
                     DQQYGGLMMWVPGTLCFWGAMTVIWFRWAARDEEGDAELAVPQEAYATAPRIIPRPPS
                     PPRDAA"
     gene            complement(9569..10078)
                     /locus_tag="VFU75_03325"
     CDS             complement(9569..10078)
                     /locus_tag="VFU75_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465891.1"
                     /translation="MSNRFNRILREGFVGGCIGAAAVATWFLLVDTIGGRPFFTPAML
                     GSAVFWGVHDPANVVIEFSRIVGYTMIHVSAFVVIGVLAAWLVMKTEEVPHAMFLVIV
                     LACFFEFGFYIFLAILAPPLLGALAWWSVAVGNGIAALGMGGYFWRMHPALAENLRRH
                     PLGETADGE"
BASE COUNT         1593 a         3354 c         3339 g         1898 t
ORIGIN      
        1 cgcggtgcca gggcgtgatt cagtgacccg gccttgagct ggtagcgggt ggtgagtgag
       61 tccgagccgg agcgtcccca gcccgccgcc agtccggcgt gcgcgtaggc gagggtgaag
      121 ttcgagtcga tgccgaaggc ctgctggtag gcgacgagcg ccgaatccca ctccgaacgg
      181 cggaggtatt gctcgcccgt caggaacgcc cgcaccgcgg gcaaggacgc ggagccgatg
      241 cccgtcgtcc gcgccagtcc caggccgcgg atccgtgttc cgccgatggc ctggagcaca
      301 tgcacggcca ccgagtcggc caggcggtcg atgcggtcct tgtgatcccg gaggtctacc
      361 gcgcccagct gattgccggt cgacacgtcg aggacgttgg cgctgaggcg gaccgagtcg
      421 gtcccggaag tgagcagcgc gccgtacacc acgatccgcg ctccggtgtt ctcgccgagg
      481 gcgcgcgcgg cgatcggatc ggcgcgtccc gaccaccgtt tcacgatcac ggtgggggag
      541 acggtgcgga gcggtccggc gccgtccagc gaacgcgcca ggatgtccac cagcccttcc
      601 cgccagagct ggagtgacgg atcgagaacg tcgaagggcg ccaccgcgac cttgtcgtcg
      661 tccagcatgc tgccaccgct ccgtcccacg atgccccaga ccagcgcgat gccggccaac
      721 gccgcgacgg ccgtgacgat ccagcgggtc cggggcttca gggccgcgac cgttccggtg
      781 cgcgtgcggg tggaatgctt cgtcccgcca ctcgtcaacg tcgccgtgcc ttcgagcacc
      841 ctcagcacct cggcggcggt ctgcgggcgg tcggcggcgt tcttctcgag acacgccatc
      901 accaggtcgg cgagctcctg ggggacctgg ggccggcgct tgccgatgtc ctcgggaggt
      961 tcggtcagat gcgcggccag cgtctgctgc gggctcatcc cctcgaacag ctgatgaccg
     1021 gagagggctt cgtaggccat ggcgccgaac gaatagatgt cggcccggtg gtccaccgtc
     1081 ggatcaccgg ccgcctgctc cggcgccatg tatgccggcg tgccgatcgc cacgccgacc
     1141 gaggtgagcc cgctcgagcc gccggcgctc gagatcgctt tcgcgatgcc gaagtcggcg
     1201 acgagcgcgt ggttccccga cagcaagacg ttgtcgggct tgatgtcgcg gtgcacgacg
     1261 ccgcgcgcat gcgcgtacgc cagcgcgtcc gcgacgtcgt ggagaatccg ctgggcgtcc
     1321 cggatgggga gggcaccctc gcgtttcacg cgcgcccgga gggactcgcc gtcgacgaac
     1381 ggcatcgtga agtagaggag gtcgccggcc gtgcccgccg tgagcagggg aacgatgtgc
     1441 ggatgctgga gtgaggcggc gagctgggtc tcgcggcgga accggtcggc gttgacgccg
     1501 gccgacagct cgggagccag caccttcacg acgaccttcc gggccagacc tacctcttcg
     1561 gcgaggtaga cccggctcat cccaccaccc cccagctccc gttcgatccg gaagcgcccg
     1621 gagagcgctg cttcgagttg ggggcgaagg tctggcatgg ggcttagaag atacccctag
     1681 tgactcttgt ccggatacgc gggtggcggg ggtaccggtg tcgggtgagc cttcatctcc
     1741 gccctgagat aggcgatcgc cgcatcgagc tgggcatctt gtccccggta cgtcgcgccg
     1801 gggagatcgt ccaccacgga gtcggggtcc acgccgtggc cttcgatcag ccaggcaccc
     1861 tcgggcccgt acacgccatt ctcggccgcc gtggcgatgc cgtgatcctc gagggtattc
     1921 gaggaggaga gccagatttc ccctccccac gtccgcgtgc cgatcacctt gcccaggccg
     1981 agccgccgga atccttcact gaaggcttcg ccgtccgagg ccgtccgttg gtcgcagagc
     2041 accacgacgt gcccgcggaa cgcccactgc atgttccagt agggcttccc gatgcggccc
     2101 tgccaataca tccacgcctt ccgcatcagc ttctcgagga cccagctgtc gatgttcccg
     2161 ccgttgttgt ggcggacgtc gatgatgagg ccctggcggt ccaggttcgg atagaagtcg
     2221 cgctcgaact gggcgatgtc gttctgcgtc atggcccgga gatggacgta ccctatggag
     2281 tcgtggctcg cggcgtctac cgcgacccgg cgcgtgtact cccactcgct gtaacgaagc
     2341 cccgcggccg caccgccgga catgggtgtc accaccacgt cccggcccgc cgtggtaccg
     2401 ggcgccttca ccctcagcaa aacctccttg ccggtctggt tctcgagcaa ggcgtcgaag
     2461 ttggggaccg agagggcggg cacgccgttc accatctgga tcacgtcgcc ttcggcgatc
     2521 cccagcgcgg gcatcgcgag cggtgagcgc tggaccggga aatcggggtc ggacttgtag
     2581 atgtgcgtga tgcgccagcc gccgtcggct tcgtcacgct cgtaggtcgc gcccagggag
     2641 cccagctgaa tctggtcgtc gccttggcgg aagtccccgc catagacgaa gatgtggagg
     2701 gccgaaagct cgctcaccat ctgcgccagc acgtccgaca gctcccctcg actcgtcacg
     2761 cgggctgcca gcggagcgta cttcgaccgc atcgcgggcc agtcaacgcc gttcatgtgc
     2821 cggtcgtaga agtagtcccg ctcgaggcgc cacgcctcgc gatacatctc ggcgaactcc
     2881 tcgcgggggt cgatcggaaa actccacccg gcgagctgca cctgcccctt ggcgtcgagc
     2941 gtggcgggtg cgctcgacgc gtctacgacg tagaggtccc cgtccttctg gatcatcagc
     3001 ttcttccggt ccgccgtgag ctcgtactgc agcatgtcat ccgcgacggt tttcggcttg
     3061 ggcgactggt tcgagatctc gagcgccatc agggcccgcg gcggtcggga ggccggccca
     3121 aaggagagat agaagagacg atcgcttccc accgagaggt tggcgtagtt gcccgccggc
     3181 accggcacct cgagcaaccg gtccgtaatg ccgtcgaagt ccacggtgac ggagacgtcc
     3241 ttcttgccgg tgtccggtgg agggagcggg gtctcgtcct tgggctggaa gggcgagcgc
     3301 aagcctttga ccagggagac gagatagatc ttcgcctgct tgtcgaagaa cggctccggc
     3361 gcgcgcgggc cccagggtcc gccgatcaga gactggaagt tccggtccga gaggaagtac
     3421 agccatttcc cgtcggcgct ccagccgggg gagtagctgt cggcccgcgg cgtggtgacg
     3481 ggcgtcgcct tcccggtcac caccgagtac aggacgatgg tcgagaacgt cgtcccgggg
     3541 cgcgtgtagg caagccacct ggagtccggg gaccaggtca gatcgtcgta gccgccgtca
     3601 ggggactcct cgatcttcgt ggtcttcttc gcggagagat cgacgagcca gagggtctga
     3661 ttcttgtcgg tgtaggcgag gtacttcccg tccggtgacg tgactccgtc ccaccgcagc
     3721 accttcgcgt cattcgtgac cggctccgcg ccgccgacgc cgttggcggg gagcctccac
     3781 cagtcggtct cgccggattc gtcggagagc accatgatgg attttccatc gggcgcgaag
     3841 cgggccgagc ggtagcggac cccctggtga cgggaggctt cgaccagccg gccctgagtc
     3901 acgggcgcga cgaacacctg gccccgcgcc gtcagcacca cgcggtcgcc attcggggac
     3961 acatgagtgg cggtgacgta atccatcggc ttcgtcaccc accgaacgcg cagctggtcg
     4021 aagtccgacg tcagggtgac cggaatcaga tgcccttgag cagtcgcgat gtcgtaaagc
     4081 cagaggtcgg cacccagctg gtagacgatc ttgccgttgg cgagtgcggg ggactggatg
     4141 tcccagccct ggtgatgggt cagctgcttg aggtcgtgac cctcggggtc catggaccag
     4201 aggttcatcg agccgtcgcg gtcggaaacg aagtacaccc ggcccgccca ccacatcggt
     4261 gtcttgctcg tgccggtgta gtccccggtc agcggcgtcg cttctgaggc gcccgcgggc
     4321 agcttccaca gactttgcgc tgtccctcct ttatagcgtt tggtgtgact gccctggaac
     4381 gcgaagcggg tgaagtagag cgtgcccccg gcgcggtcgt aggtgccgtc cgaggcttgc
     4441 acgaggggaa tggggctgcg gacgccggtc gacagatcga gtgtgaagag ccgaggctcg
     4501 gggagcgtcg acagggctcc cgacatcatc aggagcttgc catcgggcgt ccaacccacg
     4561 gggagtccgc ccccccaggt ccgtcggatg ggaacgccac cggacatggg gacggtgtag
     4621 atctcggcag gtccttcgta cgacccgacg aaggcaatgg tcttgccgtc cggggagatg
     4681 gtcgcattgg tctcttcgcc ctgaccgctc gtgaggcgcc gggccactcc acccgccgcg
     4741 tccaccatcc agaggtcccc ctccgcggtg aacaccaggg aatccccatg cagagcgggg
     4801 aaccggtagt acccgaggcc cggagcgtcc gcgacacggg tgtggaaaac ggaaacaggg
     4861 gatggaccag ggagggccgc gaaggcgagg gccgcggcga ggaggcgttg catgctgttc
     4921 tccgaaatca cgttgtgacg gcccgattga ggccgtgaaa atgcacctcg ccgtcgttga
     4981 ttgcgagccc acggcgtgct accttcttgg cgccttttcg cgaccgacta ggacgatcgc
     5041 ctccgtgcat agacactatc gcctgatcca gcgttgcatt cccggccttc tgctcctggc
     5101 cgttgtggcc ctcggagcct gtgccgggcc ctttccccaa accaccctgc accccaagtc
     5161 tgacttgggg ttcgcgatcg accatctatt caccggaatt ttcgggtggg cggtcggcgt
     5221 gttcgtggtg gtcgaggggc ttctggtcta cgcgctcttc cggttccggg ccaagcccgg
     5281 ggcggcggcg ccgcacccga cccatgggca cacggcgctc gagatcgcct ggacgctcgc
     5341 gccggtcctg atcctggtga tggtggcggt gccgaccatc cggacgatct tcgcgaccca
     5401 ggcgcccgcg ccggcgggat cgctcaagat cgaggtgatc gggcaccagt ggtggtggga
     5461 gtaccgctac ccggaccagg gcgtcgtcac ggccaacgag ctccacctcc cggtgggccg
     5521 gaccatcgcg ctggacctga cctcgaatga cgtgatccac agcttctgga tccccgccat
     5581 tgcggccaag cgtgatgtgg tgcctggcca tgtgaaccac atcgtgttca aggccgacac
     5641 ggtcggcgaa tacatgggcc agtgcgccga gttctgcggc gactcccacg ccaacatgcg
     5701 gctccgcgcg ttcgtcgaga ccgactcggc gttcaacgcc tgggtggcga accagaagca
     5761 gccgccggtg gagccccagc ccaacacctt gcaggcgacc ggcaagaacg ccttcgcgca
     5821 cggcgcctgc atcgcctgtc acaccatcca gggtgtctcg gcggggatga tcggaccgaa
     5881 cctgacccat ctggccagcc ggaccaccat cgcgggtgcg acgtatcctg acgactcgct
     5941 ggacctcgcg ctgtgggtga accatgcccc gcagcggaag cccggctccg tgatgatgga
     6001 catgaacttg cagccggcca acctgcaggc catcgtcgcc tacctgatga gcctcaaatg
     6061 accgaaccca ccggactgaa gagctggatc accaccgtcg atcacaagcg gatcggcaag
     6121 ctctacttct ggaccgcgtt ctgcttcttc ctggtgggcg gcatcgaagc gctgctcatc
     6181 cgctcgcagc tggcgcagcc caacggccac atcgtggacg cccggacgta caacgagctg
     6241 ttcacgatgc acggcaccac gatgatcttc ctggcgctga tgccgttgtc ggtggcgttc
     6301 ttcaacttca tcgtgccgct gcagatcggc gctcgcgacg tcgcgttccc gcggctcaac
     6361 gccttcagct actgggtgtt cctgctgggc gggatcttca tgaacgtgtc ctggttcgtg
     6421 gggcccctct tccacgcggg ccggctctcg gtggcgccca acggtggctg gttcggttac
     6481 gcgaacctca cggaacgcca gtactcgatc ggccccaaca tcgacttctg gctgctcggc
     6541 ctccagatcc tgggcgtctc gtccctcgcc ggcggattca acttcgtcat cacgattctg
     6601 aacctgcggg cgcccggcat gaagatgatg cgcctgccgg tgttcacgtg gatgacgctg
     6661 gtgacctcct tcctgctgat cacggcgttc cccgtgatca cggtggcgct ggcgttcctg
     6721 atgttcgacc ggttcttcgg gacgaacttc taccagctgt cggcgggcgg ggacccggtg
     6781 ctctggcagc acctgttctg gatcttcggg caccccgagg tctacatcct gattctgccg
     6841 gccatgggcg tggtgtccga ggtcctgccc acgttctcgc ggaagccgct tttcggctac
     6901 acggtcgtgg tgtactcggg catcctgatc gcgttcatgg gctggggcgt gtggagccac
     6961 cacatgttct cggtgggcct cgggcccatc gcggacgcgg tgttctcctc caccaccatg
     7021 ctcatcgcca tcccgaccgg tgtgaagatc ttcaactgga tcgccacgct ctggggcggt
     7081 cagatccggt tcgatacgcc gctgctcttt gcggtgggct tcatcgcgat gttcatcatc
     7141 ggggggcttt cgggcgtgac gcacgcgtca cccccggcgg atctgcagca gaccgacacc
     7201 tactacgtgg tggcgcacat ccactacgtg ctcttcggcg gcaccgttct ggggctcttc
     7261 gccgggatgt actactggtg gccgaagatg acgggccggc tcctgtccga gacgctgggc
     7321 aagatccact tctggggcat gctgatcggg atgaacatcg cgttcttccc gatgcacttc
     7381 atcggccttc tgggcatgcc gcggcgcgtc tacacgtatt cgccggagct cggcgtgtcg
     7441 accatgaacc tggtgtcgac gatcggcgcg ttcctgatcg ccgtctcgat gctggtcttc
     7501 atctggaacg tcatccgcac ccgcatcaag ggaaagatcg cgggcgacaa cccctggcag
     7561 ggcgccacgc tcgagtgggc gacgtcgtct ccgccggcgg tctacaactt cgcccaaatc
     7621 ccgaccgtgc actcgcgcct gccgctgtgg aacgagcccg agtcggtggc ccagagggcg
     7681 ccgagcggac atatccatgt gcccgacggt tcgtggtggc ctctcgtctc ggcggccggt
     7741 ctgccgctcc tcgcgacgtc actcatttcg ggccagctct ggctggcctt cgtggcggcc
     7801 gcggtgctcc tggtgggaat cttcaagtgg ggcttcgagc cctttgaggt ttagatgagt
     7861 cacgccgcgg cgacgcacac cgagggccac catacctcga tggggttgga cagccggaag
     7921 atggcgttct gggccttcat cggctccgag tgcctcctct tcgcgaccct gatttccacg
     7981 tacctcatct acagccaccg aagcatccag ggtccctacc cgcaacagat cctcaatatt
     8041 ccctttacgt cgttctcgac gttcgacctg ctgatgtcgt cgctgtggat ggtgctggct
     8101 ctggcggcgg tccagcggaa cgacatgaag tgggccaagg tctggctcat gggcacggtg
     8161 atcggcggcc tgatcttcct gctgggtcag gtgtgggagt tctcgcactt cgtggagcac
     8221 gggctgacgc tccagcggaa cctgttcggc acgaccttct tcgtgctcac gggcacccac
     8281 ggggctcacg tgttcgtcgg ggtgctgtgg ctcttcaccc tctgggtacg ggcgatgcag
     8341 ggcaaactga cgtccaagaa cgcgatgacg gtcgagatca ctggcctcta ctggcatttc
     8401 gtggacgtga tctggatcat catctttacc gtcgtgtacc tcattcaacc ggtctaccat
     8461 gtctgagacc cacgccagcg agcacccgca tccgaccgcc ggcctgtacc tccgggtggc
     8521 cacggtactg ttcattctca cggtgctgga agtaggcgtg ttctacgtgc ctacgttcca
     8581 ccccatcctg gtcccggccc tgttgatcct gtcggcgatc aagttctgcc tggtggtgat
     8641 gttctacatg cacctgaaga tggacaatcg cttcttcacc atgatcttcg gcggcccgct
     8701 gctcctggcc ttcgggatgg cgatggcgct gatgttcctc ttcaaggtgt tcgtattgaa
     8761 gtgacgccgg ctccctggga tcactgggac atccatccgt cggtggtgat cgggctcgcg
     8821 ctgttcggcg ggctctatct cttttgggga gggaccaagg cttcaatctg gcgccaggtt
     8881 tcgttctggg cgtcggtgct ggtgcttttc ttctcgctga acgggccgct gcacgacctg
     8941 tcggacacgt acctgttcag cgcccacatg ctgcagcacc tgatcctcac gctggcattt
     9001 ccgccccttc tgctgttcgg cacgcccgcg tgggtggtgc ggccgttgct caagccgcgg
     9061 tgggtgatgg cgctggcccg gaccatcacg aagccgttac cggccgggat catcttctcc
     9121 acgaccatca tcttctggca cttcccccgg ttctatgagc tggcgatgcg gcatcacaac
     9181 ctccacatcg tccaacatct cttcttcatc gcggcgagcg tcgtaatgtg gtggccggtc
     9241 ctgtcgcccg tgccggaact gccgcggacc tcgtacccgc tgcagatggt ctacctcttc
     9301 gtgttgggtc tgccgatgtc gctggtgggg gcgatgatca ccctggccga tacacccgtg
     9361 taccggttct acgtggaggc gccgttgcgg gtctggggcc tgtcaccgct ggcggatcag
     9421 caatacggcg gtctcatgat gtgggtgccc ggcaccctgt gcttctgggg cgcgatgacg
     9481 gtgatctggt tccggtgggc agcccgggac gaagaagggg acgccgaact ggccgtcccc
     9541 caggaagctt acgcgaccgc tccccggatc attccccgtc cgccgtctcc cccaagggat
     9601 gccgcctgag gttctcggcc agggccgggt gcatccgcca gaagtagccc cccataccca
     9661 gcgcagcgat cccgtttccg acggctacgc tccaccaggc gagcgcgcca aggaggggcg
     9721 gagcgaggat cgccaggaag atgtagaaac cgaactcgaa aaagcacgcc aggacgatca
     9781 ccaggaacat ggcgtgcggt acctcttcgg tcttcatgac gagccaggcc gcaaggactc
     9841 cgatcacgac aaacgcgctg acgtggatca tggtgtagcc gacgatccgt gagaactcga
     9901 tcacgacatt agcgggatcg tgcacgcccc agaatacggc gctgccgagc atggcggggg
     9961 tgaaaaaggg gcggccgccg atggtgtcca cgagcagaaa ccaggtggcg accgccgctg
    10021 ccccgatgca gccgccaacg aacccttcgc gcaaaatgcg attgaaccgg ttcgacatca
    10081 gcgctccttc gggcgtttcg cgcaatatat ccgccgctgt agtaacagct agattgggca
    10141 ttgtgaggtc tccgcgatgg tccgccgtca gcctgctggt cggc
//
LOCUS       DASUBP010000084         3945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1174395, whole genome shotgun sequence.
ACCESSION   DASUBP010000084 DASUBP010000000
VERSION     DASUBP010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174395"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1407
                     /locus_tag="VFU75_03330"
     CDS             163..1407
                     /locus_tag="VFU75_03330"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HEU5465892.1"
                     /translation="MSELVTITSIAGGGDGVGRLGDGRAIFVPRTAPGEQVRLGAVEK
                     RKNFARGAIGEIVTAGPARIVPACRHYVDDDCGGCQLQHLSYDAQLAAKRSIVGEALR
                     RIGKLDLPDPEIVEAAEEWRYRAKITLAVKAGHDGRGITAGFHPQNRPNAVFALHDCH
                     IADVRLMALWRALVTHLDLLPNRPAHITLRLDREGNRHVIVESSGEPWLKADEMRRAL
                     SAGDGDVICWWQPTEGAARVMAGPATGFPATAFEQVHPEMGGLARQWAVRQLGPLTDQ
                     LAWDLYGGIGDTAIALAGAGASVVSVDADEKAVTWARSRPGVERIRFIAGKTEDVLPT
                     LEDPPAAAVVNPPRAGLHWDVTLRLTGEPVPKLVYISCDPATLARDLSRLSTNYHVTG
                     VKAFDLFPQTAHVETVAVLEAA"
     gene            1404..1922
                     /locus_tag="VFU75_03335"
     CDS             1404..1922
                     /locus_tag="VFU75_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HEU5465893.1"
                     /translation="MKYFVRLAGRTMEVEVLGGAVRVDGETLDAHLAAVPGTPLHHLL
                     LGGDSWTVSAQALEGMGQWALGAVGERVEVEAVDERTQAIRTLTGKKGGPSGGGVVKA
                     PMPGLVVRVQVTEGQKVGPGTGLVVVEAMKMENELKATHPGIVKKVHVAPGAIVEKGA
                     PLVTLESVEPSN"
     gene            1984..2427
                     /gene="rplM"
                     /locus_tag="VFU75_03340"
     CDS             1984..2427
                     /gene="rplM"
                     /locus_tag="VFU75_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEU5465894.1"
                     /translation="MKTFVLKPEEIERRWWVVDAAGQPLGRLASRVAQVLRGKHKPTF
                     TPHVNGGDCVIVVNAEAVKLTGNKPAQKEYFRHTGYMGHERFTPVAKLLAKHPDRVIE
                     KAVWGMLPKTTLSRQKVKQMLKVYAGPAHPHAAQQPQPMTLAGAK"
     gene            2463..2831
                     /gene="rpsI"
                     /locus_tag="VFU75_03345"
     CDS             2463..2831
                     /gene="rpsI"
                     /locus_tag="VFU75_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013938599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEU5465895.1"
                     /translation="MGRRKVAVARVYLTPGSGKWNINGRTLGDYFPRPSLVSHIQQSF
                     TVTDTLGAFDVRARVNGGGMTGQAGAIRMAIARALCLADERHRKKLREQGLLTRDPRA
                     VERKKPGRAGARKRFQFSKR"
     gene            2963..3910
                     /gene="rpsB"
                     /locus_tag="VFU75_03350"
     CDS             2963..3910
                     /gene="rpsB"
                     /locus_tag="VFU75_03350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU5465896.1"
                     /translation="MAQPQLQDLLEAGVHFGHQTRRWNPKMRRFIFGERNGIYIIDLN
                     QTLERIDVAYSFVRDLVARNGTILFVGTKKQTQDPVARYAAQCGMPYINERWLGGMLT
                     NFNTIASRVKKMQEYERMRAAGDFDAMPKKEALILSRELEKLQRNLGGIRNMARLPEA
                     VFVIDTKKEHIAVTEANKLGLPIVAVVDTNCDPDLIQYVIPGNDDAIRAGSLMCRVIA
                     DAVEEGRFVASRRAARSGAPVAEAPRALTPEEEAEREEQQREARRQAALAQAEREARL
                     TAAREQAEQAATEEAAASEPAAPAAAGDDTTDATPTEQE"
BASE COUNT          684 a         1276 c         1391 g          594 t
ORIGIN      
        1 ctctgctccg ctgtccgatg ctctcgtgaa gccggtggcg gtggtggcgg cgatgctggc
       61 tgacgagcgg agatcagggc acgctgcgga gagtacggct cccggctccc ggctcccgac
      121 aagtccgtgg ctcgacgcgg cgcgtcgcga aggaacgggg cagtgagcga actcgtcacg
      181 atcacctcga tcgccggtgg cggcgatggc gtgggccgcc tgggggatgg gcgagccatc
      241 ttcgtcccgc gcaccgcccc cggtgagcag gtccggctcg gcgcggtcga gaagcggaag
      301 aacttcgcgc gcggtgccat cggcgagatc gtcacggccg gaccggcgcg catcgtcccc
      361 gcctgccgcc attacgtgga cgacgactgc ggcgggtgtc agctccaaca cctctcctac
      421 gacgcgcagc tcgcggcgaa gcgctccatc gtgggtgagg cgctccggcg gatcggaaag
      481 ctcgatctgc cggaccccga aatcgtcgaa gccgccgagg agtggcggta ccgggcgaag
      541 atcacgctcg cggtgaaggc gggccacgac gggaggggca tcaccgccgg gttccatccc
      601 cagaatcgcc ccaacgcggt cttcgccctg catgactgcc atatcgccga cgtgcggctc
      661 atggcactct ggcgcgcact cgtcacgcat cttgatcttc tgcccaaccg gccggctcat
      721 atcacgctgc gcctcgatcg agagggcaat cgccacgtca tcgtggagtc gtccggggag
      781 ccgtggctca aggccgacga gatgcgaagg gcgctttcgg cgggagacgg cgacgtgatc
      841 tgctggtggc agccgacgga gggagcggct cgcgtgatgg cgggtcccgc caccggtttc
      901 ccggcgaccg ccttcgagca ggtgcacccc gagatgggtg ggctggctcg acagtgggcg
      961 gtgcggcaat tggggccgct gaccgaccag ctcgcctggg atctgtatgg gggcatcggg
     1021 gacacggcga tcgcgctcgc cggggccggg gcgtcggtgg tcagcgtgga tgccgacgaa
     1081 aaggcggtca cctgggcccg gagccgcccc ggcgtggagc ggatcaggtt catcgcggga
     1141 aagaccgaag atgtacttcc cacgctcgaa gatccgcccg ccgccgcggt ggtgaacccg
     1201 ccgcgtgcgg gactgcattg ggacgtcacg ttgcggctga ccggtgagcc cgtgcccaag
     1261 ctggtctaca tctcttgtga tccggctacc ctcgcccggg acctttcgcg cctgtccacc
     1321 aactaccatg tcaccggtgt gaaggcattc gacctcttcc cgcagacggc gcacgtcgag
     1381 accgtggccg ttctggaggc cgcgtgaagt acttcgtgcg tctggcgggg cggaccatgg
     1441 aggtcgaggt gctgggcggg gcggttcggg tggacgggga gactctcgat gcccatctcg
     1501 ccgccgttcc gggcaccccg ttgcatcacc tgctgctggg cggcgactcc tggacggtct
     1561 cggcccaggc cctggagggg atggggcagt gggcgttggg ggcggtgggg gagcgggtcg
     1621 aggtcgaggc ggtggacgaa cgcacccagg ccatccggac gctgaccggc aagaaggggg
     1681 ggccatcggg gggtggggtg gtgaaggccc cgatgccggg tctggtggtg cgcgtgcagg
     1741 tgactgaggg ccagaaggtt ggccccggca cggggttggt ggtggtcgag gcgatgaaga
     1801 tggagaatga gctgaaggcg acccaccctg ggatcgtcaa aaaggtgcac gtcgcccccg
     1861 gtgccatcgt cgaaaagggg gctcccctcg tcaccctcga aagtgtggaa ccctccaatt
     1921 gacacgtgct tagagcccgg ttagagttct agtctgtcta ttttcgggct cttgaagagg
     1981 accatgaaga ccttcgttct gaagccagag gaaatcgaac ggcgttggtg ggtggtggat
     2041 gccgccggcc agcccctggg gcgtctcgcc tcgcgggtgg cgcaggtcct gcgcggcaag
     2101 cacaagccta cgttcacgcc tcacgtcaac ggcggtgact gtgtcatcgt cgtcaacgcc
     2161 gaagccgtga agctgaccgg caacaagccg gcgcagaaag agtacttccg gcacaccggt
     2221 tacatggggc acgagcggtt cacgccggtc gccaagctcc tggccaagca tcccgaccgc
     2281 gtcatcgaga aggcggtgtg ggggatgctg ccgaagacca cgctctcccg gcagaaggtg
     2341 aagcagatgc tgaaggtgta cgcgggaccg gctcatcccc acgcggcaca gcagccgcag
     2401 cccatgacgc tggcaggtgc caagtgagtt ccgccatcac gccggagctt cgttggcacg
     2461 ccatcggccg ccgcaaagtt gcggtcgcgc gtgtgtatct cacgccgggt tcgggaaaat
     2521 ggaacatcaa cgggcgcacg ctgggtgact acttcccccg gccgagcctg gtgtcgcaca
     2581 tccagcagtc gttcaccgtc accgacacgc tgggcgcctt cgatgtgcgc gcgcgcgtca
     2641 acggcggcgg catgacgggg caggccggtg cgattcgcat ggcgatcgcg cgggcgctct
     2701 gcctggccga cgagcgccac cggaaaaagc ttcgcgagca gggcctcctc acccgcgatc
     2761 cgcgggccgt ggagcggaag aagccgggcc gggcgggcgc gcggaagcgc ttccagttta
     2821 gcaagagata gaacgcatta gacggctaga cggttagacg gctagcctaa cccacacgtc
     2881 gcttgatcct gtcagggtct ccccacgggg agtgagacgg gaaaagagag cggcggcgga
     2941 ataacccaag aaaggatttg ctttggcgca gcctcaactg caggacctgt tggaagccgg
     3001 tgtccatttt gggcatcaga ctcggcgctg gaaccccaag atgcggcggt tcatcttcgg
     3061 tgagcgcaac gggatctaca tcatcgatct caatcagacc ctcgagcgca tcgatgtggc
     3121 ctactcgttc gtccgcgacc tggtcgcccg gaacggcacg atcctgttcg tcggcaccaa
     3181 gaagcagacc caggacccgg tcgcccgcta cgcggcccag tgcggcatgc cctacatcaa
     3241 cgagcgctgg ctcggcggca tgttgaccaa cttcaacacc atcgccagcc gggtcaagaa
     3301 gatgcaggag tacgagcgga tgcgggccgc cggcgacttc gacgccatgc cgaagaagga
     3361 ggcgctcatc ctgtcgcgcg agctcgagaa gctgcagcgc aacctcgggg gcatccgcaa
     3421 catggcccgg ctccccgagg cggtgttcgt gatcgacacc aagaaggagc acatcgcggt
     3481 caccgaggcg aacaagctcg gcctcccgat cgtcgccgtg gtcgacacga actgcgatcc
     3541 cgacctgatc cagtacgtca tccccggcaa cgacgacgcc atccgggccg gcagcctcat
     3601 gtgccgggtc atcgccgacg cggtcgagga agggcgcttc gtcgcctccc gccgggccgc
     3661 ccgctctggt gcaccggtcg ccgaggcccc ccgcgccctc acccccgaag aggaagccga
     3721 gcgggaggag cagcagcgcg aggcccgtcg tcaggccgcc ctggcccagg ccgagcgtga
     3781 agcccggctg acggctgctc gcgagcaggc cgagcaggcg gcgaccgagg aggccgccgc
     3841 ctccgaacct gccgcccccg ccgccgcagg cgacgacacc acagacgcaa cccccaccga
     3901 gcaggagtag aagtggcaga tttcaccgcc caggacgtaa aggcg
//
LOCUS       DASUBP010000085        14357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_151886, whole genome shotgun sequence.
ACCESSION   DASUBP010000085 DASUBP010000000
VERSION     DASUBP010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 14357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14357
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151886"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VFU75_03355"
     CDS             <1..225
                     /locus_tag="VFU75_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465897.1"
                     /translation="REIRDLLSGIGVRTLAALTKGDKLPRGAGRKREAELAAELELDD
                     DQMLLTSAKTGAGVADLREALTALIGKAAG"
     gene            222..824
                     /locus_tag="VFU75_03360"
     CDS             222..824
                     /locus_tag="VFU75_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465898.1"
                     /translation="MNHRTALLIGVLGALAAPQKSRAQDTLPVGFGTLKRDNVVISFS
                     TGPLQIQILPLDESVTRLLAPDTYKSLTDLIKQRQSDISDQAQRAGVRNPTLVMVTFF
                     GTVSQARFVPEDVDLTSRGRLFRPVGIVPLSPQWGGQQLDARQQATAIYLFESGIAWG
                     ESLTASYEGMTNDAWSKTIQTLNTERMRVLSRASARAPAH"
     gene            832..2952
                     /locus_tag="VFU75_03365"
     CDS             832..2952
                     /locus_tag="VFU75_03365"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5465899.1"
                     /translation="MNTQIGLVLGALAIGVLAAAAVYLWIRLARVEEDRRRIADELNR
                     RLSELFSLQELSYVLSDSLQLDRIVDHVVRYAMRFLEARGALVALTGERVTEPLTVAA
                     ADGTLAEQRGATIRRSDAGLVVRSVGRERLELVRNSGGGSVVLIAGINAEAAAAVPLR
                     AHGAVVGTLVVADPRGGQFASEDLRLISTLATHTAVVIANARFFDLVRRAKEEWETAF
                     DAMSEGLAVVDETGRITRANRALASLLSREIAEVQGHELAPSLVEHPAPLNELMELAR
                     RGGRPVPLVARSRVGRAVRINAARMPPVGTASHLVVLVEDVTEQQTMESQLIQSEKLA
                     AVGQLVSGVAHELNNPLTSIAGLSEFLLEQKELGENDRGHLRVIHEQAERAGRIVRNL
                     LTFARKAPAERERVDVTDVLQRTLLLMSYDLRLKDVTLEKDFAQLLPDILGDRHAIQQ
                     VVLNLLTNAAQAVSGNPPDRPRTVKASTWFDGKVHLRIQDSGPGVSEEVARQLFTPFF
                     TTKEPGQGTGLGLSISYSIVEAHGGKISVEPAPAGGGAAFHVEFPPAPADTPAKLDLH
                     RTPAPPTRSSRVSKSARPVRSILLVDDDQAVRRMVQALFGREGHAVEVARNGQHALEL
                     AANRAFDLIIADGRSASTGRLLVEELAERMPHLKSRTLVATGDVRPATDETLTRLGLR
                     YVRKPFNLRDLRDEAARVWAATES"
     gene            complement(2877..3263)
                     /locus_tag="VFU75_03370"
     CDS             complement(2877..3263)
                     /locus_tag="VFU75_03370"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HEU5465900.1"
                     /translation="MNPFYHRIYRVVRHIPKGRVATYGIVARLAGRPGAARQVGWALS
                     ALPEDSDVPWWRVINAAGRISLSGADHGAVVQRALLLREGVKFAPGGAVNLSTFGWPA
                     EAYDSVAAHTRAASSRRSRRLKGLRT"
     gene            complement(3274..3741)
                     /locus_tag="VFU75_03375"
     CDS             complement(3274..3741)
                     /locus_tag="VFU75_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEU5465901.1"
                     /translation="MTDIKVMFVDTFVLRGAGAGLRVLVLRRAAGRTRAGAWEIVHST
                     IEPGETPVQTARRELREETGLTPVSLYNLSRTEAFYQHDTDVLAVIPVFAAFVAEGAP
                     VTIADEHDAYEWLAPADAGARLAWPRERRALEDVLSMVGGGGAGLLDGVLRVS"
     gene            complement(3747..5990)
                     /locus_tag="VFU75_03380"
     CDS             complement(3747..5990)
                     /locus_tag="VFU75_03380"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HEU5465902.1"
                     /translation="MPETTFPDEWLMTSLEGVITPELLEELRAKAEPSRTLWEMIVSR
                     HIATDDGILEKLSQRYRMKVADVALVDPHVKDTLPEQLVRRYRVLPVRITDSFCEVAT
                     ANPFDLDAEKSIAFATGREVRMLLLSPAKIAEKLDELYRPDKTLDKLLEGMDESGDLI
                     HLAEEAPDELNVSEEEAKSRPVVKLVDLILHEGILARASDIHIEPEEGGVAVRYRIDG
                     VLRQQMKIPRTAGLPLISRIKIMSSLDIADRLRPQDGRARVAVNGQPIDLRVSTLPAA
                     LGEKVVVRILDSRATVKSLDMLGLNPGEAEAINHLLENHEGIILVTGPTGSGKTTTLY
                     SMIRQIQSEGVNIVTVEDPVEYRMQGIVQVQVQEKAGLTFASALRSILRQDPNVVLVG
                     EIRDKETAQIATQASLTGHLVLSTLHTNDAPNAVTRLVDIGVEAYKIAAALRGVVAQR
                     LMRKLCPTCKEVWMEAPPDRLRKWIPTGTPLYRAAGCPDCAMTGYRGRFSIIEVLHVG
                     PEVERRIASGQTADRIAAAARRAGMKTLWESGLAHVLRGESTVDELLRVVEVPSEEEE
                     SPPDTPAPAPSRSGVGRPSPAAGVAAPPVERVERSTDHFELLEEPAPPRATGTHGAAA
                     LKVLLVDDEDSLRKVMKDLLERDGYDVAEARDGVQALDQVDRVGPDIIVLDLNLPGLD
                     GYGVLSHLRSRPATAGIPVIVLTAKGDEDNEVRVFELGADDFLTKPFRARALSARLEA
                     VLGRRRH"
     gene            complement(6042..8129)
                     /locus_tag="VFU75_03385"
     CDS             complement(6042..8129)
                     /locus_tag="VFU75_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465903.1"
                     /translation="MARREAQKERVHYPAMRALLLLAMAGAPLRLAAQDWNAPPALAL
                     VSRGIQRRGATTADTALHDYKAHAHGFVFFLGAFGNGLNDAPRLIKTDQLELELYWKA
                     PGASKQRIVGWRNAAQLPTDINYHRDHLGIIQNNFGRAIRLGEGDEVQDVPHPLSPGA
                     QLDYDYALGDTQLVSMPGRTVRVVAVHVRPKEFSQPRIVGTLYLDVSTAALVRMAFDF
                     TPAAYRDPELEDVSVVLDDALWDGRFWLPYRQEIEIRRRVNWLDFSARGIIRGRWEIG
                     DYAFNLGLADTWFAGEEISSVPPAELDSFPWPNTLAQAMGSVETPARADDLERLRAEA
                     ARIAGTHVMTGLSPTRLGVRSLSDLLHVNRVEGLTAGAGLTQRVGGTGIEVRARSSYG
                     FGDRRVKGRGDAVWTSPVGIFTLSGYREVRDVSDHPVISPLFNSINTQEWGRDYGDYY
                     LADGGGLSFHRPLGTQGEWTTSVARERVGSLPAVGWPAQDSYAPNPQLGMGHLDIARV
                     TVRRRPAGLGEDVRRDLAGGISVEGGRVDGGDEYWRLSGDGTALLSLATTRVVMRGEF
                     GLASPNLPAHRAFVLGGRGTLLGDDYRSWGGRRAALVNVEWRVPVPFLSLSAGPYTRT
                     PSHIVIAPYVAAGWTDEPVAGTPWVATPGTRMTLGLALEWLGLLRLEGGYGAESRKAH
                     VALDVMRDFWGLL"
     gene            8094..8657
                     /locus_tag="VFU75_03390"
     CDS             8094..8657
                     /locus_tag="VFU75_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465904.1"
                     /translation="MYALLLGLAACHNSAAPLNVIHRKDLLALARSDSADPIDRTLVF
                     QNNKTVSISWLNSDTLGSTFATFHFYLQSVRSRNDTILADTSTVTLNASATPGVFGFT
                     LLPTSVVFNIAGSPTIDVSYARYADFSVIDSAPSKYPNPAAFAQALALYYEYGPDQWR
                     ALNTGNLSNVASSALSQPGTYLLAAPK"
     gene            8654..10153
                     /locus_tag="VFU75_03395"
     CDS             8654..10153
                     /locus_tag="VFU75_03395"
                     /inference="COORDINATES: protein motif:HMM:NF012640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HEU5465905.1"
                     /translation="MSDARSDFLTRAAAGGMVPLVREVLLDADTPVAAYAKIAAGPFG
                     FLLESLVGGERWARYTFLGTEPREVWRYRGRSVERWTPKDGWTAQGQVEAPLEHLAGL
                     LRHVPAVEAPGLPRFTGGAVGYLGYELIRSVERLPHPPQDTLGLADAVLMITDTVVIL
                     DNLYGRASIVATVEVPAGAPARERERLADEAERRVNALVARLERPHTLPPLRMPAHPT
                     PVPTTSRYPVERFEADVGRILEYIKAGDAFQVVLSRAQEAAGPVDALRLYRFLRALNP
                     APYLFFLQLDDCALVGSSPETLVRVEGGEVTVRPIAGTAPRGASPPEDERLGGALADD
                     PKERAEHLMLVDLSRNDVGRVAAYGTVRVTNLMVLERYSHVQHLVSEVRGKLRAGYDA
                     LDALRACFPHGTVTGAPKVRAMEIIDELEPEARGPYAGAVGYIGWGAKSLDTAIAIRA
                     ALVLKDRVVVRAGAGIVADSDPGREFAETEHKAQAVLKALALARAQGPG"
     gene            10164..11786
                     /locus_tag="VFU75_03400"
     CDS             10164..11786
                     /locus_tag="VFU75_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HEU5465906.1"
                     /translation="MFKLLSISGEQSIELAAGKTLVVGRAVTSDVPIYDPTISRRHAE
                     ITLTDAGVRIKDLGSSNGTFLNGAKVTDGDAAENDVVTFGKVAFKVVPITPPARAAVT
                     TPAPPTSNKETKGSGVPGGTIVRALPMSPSGNVPAIKEIGPSGGHLKVDAKDLYERQS
                     QKLKLLLDISQELSKQQDLDRVLEKVVDLTFQVMSVDRVSILLLDTKTNELVPRISRS
                     RAGDAQAAKMVPQSIARKVVDDRVAILSDNTAADDRFKGKSIVIQSVRSAMCTPLLSN
                     DQKVLGILYVDNQTAIHSFQDEDLDFLMAFGGLTAFSIERSQLADRLRREAMVLSNFQ
                     RYFAPNVAAQIVQQEGSVQLGGDKRPVVIFFSDIRGFTPMSETMSPDDVAHLLTEYFT
                     EMVDLVFEHSGSLDKFMGDAIMALWGAPIAHEDDADRAMKCAMDQLAALEKMNVKWKE
                     KGRPDIKIGIGINFGEVFAGNFGSDRRLEYTVIGDAVNTASRLCSNAGANEILISETF
                     YKALKHPPKVEALEPMQVKGKTKKVPVYRVKR"
     gene            complement(11768..12355)
                     /locus_tag="VFU75_03405"
     CDS             complement(11768..12355)
                     /locus_tag="VFU75_03405"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HEU5465907.1"
                     /translation="MTDTPVLVLASASPRRRQLLEMLGIEHRVVPPDVDEAHRAGEAP
                     ADYVVRLARDKARAVSGRDPRPLVLAADTTVILGDELFAKPATVGEAVEMLGRLAGRT
                     HRVYTGVALAQGERVETALDVSDVTFRRLSREQIASYVATGEPMDKAGAYAIQGKGAA
                     LIDGIRGDFFGVMGLPLRLVLDLLERFGKPYRFTR"
     gene            complement(12352..12795)
                     /gene="dtd"
                     /locus_tag="VFU75_03410"
     CDS             complement(12352..12795)
                     /gene="dtd"
                     /locus_tag="VFU75_03410"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: protein motif:HMM:TIGR00256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HEU5465908.1"
                     /translation="MRVVLQRVSRGAVRVAGETVGTVGRGFVALVGFAPGDTDETLKW
                     MADKILGLRAFSDAEDKMNLALADVNGGVLVVSQFTLYGDASKGRRPSFIGAAPPEQA
                     HALYDRFVAVLREHAGAIPVATGQFQAMMDVELVNDGPVTLILEK"
     gene            complement(12792..>14357)
                     /gene="glmS"
                     /locus_tag="VFU75_03415"
     CDS             complement(12792..>14357)
                     /gene="glmS"
                     /locus_tag="VFU75_03415"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEU5465909.1"
                     /translation="DCGGTIAVVHNGIIENAGALRTALTKRGHVFRSETDTEVLAHLI
                     EELHTKGAPLTESVAGALREVEGTYGIAVVSGREPDTIVAARKGSPLLVGVGSGENFI
                     ASDASAVLSHTRSVVYLDDGEIAVIRPTEYRILDHHTVEKEKTVTHVEWDLATVERGG
                     YAHFMLKEIMEQPESLRNTLRGRLLEDDGDVRLGGLNISDEDLLRINRIVITACGTSW
                     HSALIGEYMIEELARIPTEVEYASEFRYRNPIVDERTLVIGISQSGETADTLAALREA
                     KRRGARTIGLVNVVGSTIAREVDGGIYLHAGPEIGVASTKAFTSQIAALALLTLKLGR
                     LRALSILQGREVVKALAKLPELVEKVVAKAAQVEQIAERLQRTQNVLYLGRGYNFPVA
                     LEGALKLKEISYIHAEGYPAAEMKHGPIALIDDLMPVVFVAPRDAVYQKVVSNIEEVK
                     ARGGRVIAVVTEGDTGVARLADHKIEIPETLDIFTPILSVLPLQLLAYYIAVRRGCNV
                     DQPRNLAKSVTVE"
BASE COUNT         2311 a         4998 c         4769 g         2279 t
ORIGIN      
        1 cgcgagatcc gtgacctgct ttcgggcatc ggggtgcgga ccctggcggc gctgaccaag
       61 ggcgacaaac ttccgagggg cgcggggcgc aagcgggaag cggagctggc cgcggagctg
      121 gagctggacg acgaccagat gctcctcacc agcgccaaga cgggtgccgg cgtcgcggac
      181 ctccgcgaag cgctgacggc gttgatcgga aaggcggcgg gatgaaccac cggacggctc
      241 tgctcatcgg cgtgctcggc gcgctggcgg ctccacagaa gagccgcgcc caggacacgt
      301 tgcccgtggg attcggcacg ctcaagcggg acaacgtggt gatcagtttt tccacgggtc
      361 ccctgcagat ccagatcctg cccttggacg agtccgtgac ccggctcctc gcgcccgaca
      421 cctacaagtc cctcacggat ctcatcaaac agcgccagag cgacatcagc gatcaggcgc
      481 aacgcgccgg cgtgcggaac ccgaccctcg tgatggtcac cttcttcggc acggtatccc
      541 aggcgcgatt cgtccccgag gacgtcgacc tcaccagccg cggccggctc ttccgccccg
      601 tcggcatcgt gccgctctcc ccgcagtggg gaggccagca gctcgacgcg cgccagcagg
      661 ccacggcgat ctacctgttc gagtccggca tcgcgtgggg cgagtccctc accgcgagct
      721 atgaggggat gaccaacgac gcgtggtcca agaccatcca gacgctgaat accgaacgga
      781 tgcgcgtgtt gagccgcgcc tccgcccgag ccccggcgca ctgatccgcc cgtgaacacc
      841 cagatcggtc tggtgctggg tgcgctggca atcggggtgc tggccgcggc cgccgtctac
      901 ctctggatcc gcctcgcgcg cgtcgaggaa gaccggcgcc ggatcgccga cgagctgaac
      961 cgccgtctct ccgagctctt ttcccttcag gagctctcgt acgtcctctc cgactcgctc
     1021 cagctcgacc gcatcgtcga ccacgtggtc cggtacgcca tgcgctttct cgaagcgcgc
     1081 ggcgcgctcg tcgccctcac gggggaacgc gtcaccgaac ccctgaccgt cgccgccgcg
     1141 gacggcacgt tggccgaaca gcggggtgcc accatccgcc gctccgatgc cgggctcgta
     1201 gtccggtccg tcggccgcga gcgcctggag ctggtccgca attccggtgg cgggtcggtg
     1261 gtcctcatcg ccgggatcaa cgccgaggcc gccgccgccg tgccactgcg cgcgcatggt
     1321 gccgtggtcg gcaccctcgt ggtggccgat ccgcgcggcg gacagttcgc gtccgaagat
     1381 ctgcggctca tctcgacgct cgccacccat accgccgtcg tgatcgccaa cgcgcggttc
     1441 ttcgatctcg tcaggcgcgc caaggaagaa tgggaaaccg ccttcgacgc catgtcggag
     1501 ggtctggcgg tcgtcgacga gacgggccgg atcacccgcg ccaaccgcgc gctggcctcg
     1561 ctcctgtccc gcgagatcgc cgaggtccag ggacacgagc tggcaccgtc cctggtggag
     1621 caccccgccc ccctgaacga gctgatggag ctcgcgcgcc gcgggggccg ccccgtcccc
     1681 ctggtcgccc gctcccgggt cgggcgcgcg gtgcgcatca acgcggcgcg catgcccccc
     1741 gtggggaccg cctcccatct ggtcgtgctg gtcgaggacg tgaccgagca gcagacgatg
     1801 gaatcccagc tcatccagag cgaaaagctg gcggcggtcg gtcagctcgt ttcgggcgtg
     1861 gcgcacgagc tcaacaaccc cctgacctcc atcgccggac tctcggagtt tctgctcgag
     1921 cagaaagagc tgggcgagaa cgaccgcggc catctccgcg tgattcacga gcaggcggag
     1981 cgcgcggggc ggatcgtccg caacctgctc accttcgcgc ggaaggcgcc cgccgagcgg
     2041 gagcgcgtgg acgtcaccga cgtgctccag cgcaccctgc tgctcatgag ctacgatctg
     2101 cgtctgaaag acgtcacgct ggaaaaggac ttcgcccagc tcctgccgga tatcctgggg
     2161 gaccggcacg ccattcagca ggtggtgctg aacctcctca ccaatgcggc ccaggcggta
     2221 tccggcaatc cgcccgaccg ccctcgcacc gtgaaggcgt cgacctggtt cgatgggaaa
     2281 gtccacctcc ggattcagga ctccggtccc ggcgtgtccg aagaagtcgc ccgtcagctg
     2341 tttacgccgt tcttcaccac caaggaaccc ggccaaggca cggggctcgg cctgtcgatc
     2401 agctattcca tcgtcgaagc acatggcggg aagatttccg tggagcccgc gcccgcgggg
     2461 ggcggagcgg ccttccacgt cgagttcccc cccgcccccg cggacacccc ggccaagctg
     2521 gacctgcacc ggactccagc cccgcccacc cgctcttcga gggtctccaa gagcgcacgc
     2581 cccgtgcggt cgatcctgct ggtcgacgac gatcaggcgg tacggcggat ggtccaggcc
     2641 ctgttcgggc gggaggggca cgcggtcgag gtggcgcgaa atggacagca tgcgctggag
     2701 ctggcggcca atcgggcgtt cgatctgatc attgcggacg ggaggtccgc gtcgactggc
     2761 aggctactgg tggaggagct ggcggagcgg atgccgcatc tgaaatcgcg gacgttggtg
     2821 gcgacggggg acgtccggcc cgccaccgat gagaccctga cccggctggg actgcgctac
     2881 gtgcgcaaac ctttcaacct gcgcgatctc cgggacgaag ccgcgcgggt gtgggccgcc
     2941 accgagtcgt aagcctcggc gggccaaccg aaggtcgaga ggttgacggc tcctcccggc
     3001 gcgaacttca caccctcccg caacagcagc gcccgctgta ccaccgctcc atgatcggcg
     3061 cctgagagcg agatcctgcc ggcggcgttg atcacgcgcc accacggaac atccgagtct
     3121 tcgggaagcg ctgaaagtgc ccacccgacc tgccgcgccg cgccggggcg tccggcaaga
     3181 cgggcaacga tgccgtacgt cgccacacgt cctttcggaa tgtgacgcac cacgcggtag
     3241 atccggtgat agaaggggtt catgcggctc ctgctagctt acgcgaagaa cgccgtccag
     3301 caagcccgca cctccacccc ccaccattga caaaacgtcc tccagtgctc tgcgctcccg
     3361 cggccaggcg agccgcgcgc cggcgtcggc gggcgcgagc cattcgtacg cgtcgtgctc
     3421 gtccgcgatc gtgaccggcg cgccctccgc gacgaacgcg gcgaacacgg ggatgaccgc
     3481 gagtacatcc gtgtcgtgct ggtagaacgc ttcggtgcgt gagagattgt agagcgagac
     3541 gggggtgagt ccggtctcct ctctcagctc ccggcgcgcc gtctgcaccg gcgtctcacc
     3601 gggctcgatg gtgctgtgga cgatctccca ggctccggcg cgcgtgcgtc ctgccgcgcg
     3661 tcgcaagacg agcacccgga gccctgcccc cgcgccacgc agcacgaagg tgtccacgaa
     3721 catcactttg atatcggtca tcccgactag tgccggcggc ggcccaacac cgcttccagg
     3781 cgcgccgaga gggcgcgggc gcggaagggc ttggtgagga agtcgtcggc gccgagctcg
     3841 aagacgcgca cctcgttgtc ctcgtcgccc ttggcggtca ggacgatgac ggggatgccc
     3901 gcggtggccg gacgggaacg gagatgcgac agcacgccgt acccgtcgag tcccggcagg
     3961 ttcaggtcga ggacgatgat gtccgggccc acgcggtcca cctggtcgag agcctggacg
     4021 ccgtcacggg cctcggccac gtcgtatccg tcgcgctcca gcaggtcctt catcactttt
     4081 cgcagcgagt cttcatcgtc cacgaggagg accttgagcg ccgcggcccc atgcgttccg
     4141 gtggcgcgcg gcggcgcggg ttcttcgagc agctcgaaat ggtcggtcga acgctccacc
     4201 cgctcgaccg ggggtgccgc gactccggca gccggggagg gccgtcccac gcccgagcgc
     4261 gacggggccg gggccggtgt gtcgggagga ctctcctcct cctcggaagg gacctcgacc
     4321 acccggagca gctcgtccac ggtagactcg ccccgcagca catgcgcgag ccccgactcc
     4381 cacagcgtct tcatgcccgc ccgccgggcc gcggcggcga tccggtccgc ggtctggccc
     4441 gatgcgatcc gccgttcaac ctcgggccct acgtgcagca cctcgatgat cgagaagcgc
     4501 ccgcggtacc cggtcatggc gcagtccggg catcccgccg cccggtagag gggcgtcccc
     4561 gtgggaatcc acttccggag ccggtcgggg ggcgcctcca tccacacctc tttgcaggtg
     4621 ggacagagtt tccgcatgag gcgctgggcc acgacccccc ggagcgccgc ggcgatcttg
     4681 tacgcctcca ccccgatatc caccagacga gtgaccgcgt tgggagcgtc gttggtgtgg
     4741 agcgtcgaca gcaccaggtg acccgtcagg gacgcctggg tcgcgatctg ggccgtttcc
     4801 ttgtcgcgga tttccccgac gagcaccacg ttcgggtcct gacgcaggat ggaccggagg
     4861 gccgacgcga acgtcagtcc ggccttctcc tgcacctgga cctgcacgat cccctgcatc
     4921 cggtattcca ccgggtcttc gacggtcacg atgttcacgc cttcactctg gatctggcgg
     4981 atcatcgagt agagcgtcgt cgtcttgccg gacccggtcg gtccggtcac caggatgatg
     5041 ccctcgtggt tctcgagcag gtggttgatc gcctcagcct cgccggggtt gaggcccagc
     5101 atgtcgagcg atttgacggt ggcccgggaa tcgaggatcc ggacgaccac cttctcaccc
     5161 agcgcggcgg ggagcgtgga gacgcggagg tcgatgggct ggccgttcac cgccacccgt
     5221 gcgcggccgt cctgcggacg cagccggtcg gcgatatcga gggacgacat gatcttgatg
     5281 cgggaaatga ggggcaggcc ggcggtgcga gggatcttca tctgttgccg gagcacaccg
     5341 tcgatccggt accggaccgc gacgccccct tcttccggct cgatatggat gtccgacgcc
     5401 cgggcgagga tgccctcgtg cagaatcagg tcgaccagct tcaccaccgg ccgcgacttc
     5461 gcctcctcct cactgacgtt gagctcgtcg ggcgcttcct cggccaggtg aatcaggtca
     5521 ccggactcgt ccatgccttc gagcagcttg tccagcgtct tgtccggccg gtacagctcg
     5581 tcgagcttct cggcgatctt ggcgggcgag aggaggagca tccggacctc acgtccggtg
     5641 gcgaacgcga tggatttttc ggcgtcgagg tcgaacgggt tggcggtggc gacttcgcag
     5701 aatgagtccg tgatgcgtac gggcagaacc cggtaccgcc ggaccagctg ctcgggcaac
     5761 gtgtccttga cgtgcgggtc caccagggcg acgtcggcga ccttcatgcg gtaccgctgg
     5821 gaaagcttct cgagaatgcc gtcgtcggtg gcgatgtgcc gggagacgat catctcccag
     5881 agggtgcggg acggctcagc cttggcccgc agctcctcca ggagctcggg cgtaatgacc
     5941 ccttcgagcg aggtcatcag ccattcatcg gggaacgtgg tttcaggcat gtgctttgag
     6001 aaacctagcc gcgcctgagg ccccgtccag cccgaaacgc ttcagagcag accccagaaa
     6061 tcgcgcatca cgtcaagtgc cacatgcgcc ttcctgcttt cggcgccata cccaccctcg
     6121 aggcgcaaga ggcccagcca ctcgagcgcg agaccgagcg tcattcgcgt gcccggggtg
     6181 gcgacccacg gtgtcccggc gacgggctcg tcggtccacc cggccgcgac atagggcgcg
     6241 atcacgatgt gcgacggcgt gcgcgtgtag ggtcccgccg agaggctcag gaagggcacg
     6301 gggacccgcc attcgacgtt cacgagcgcc gcccgccgtc ctccccagct gcggtaatcg
     6361 tcacccagga gtgtcccgcg gccacccagt acgaacgcgc ggtgcgcggg aagattcggc
     6421 gaagcgagcc caaactcgcc gcgcatgacg acgcgcgtcg tcgcgagtga aagaagcgcg
     6481 gtgccgtccc cggacaggcg ccagtactcg tcccccccgt cgacccgtcc accctcgacg
     6541 gaaattcccc ccgccagatc gcgacggacg tcctcaccga gccccgctgg acggcggcgg
     6601 accgtcaccc gcgcgatatc gaggtgtccc atccccagtt ggggattcgg ggcatacgag
     6661 tcctgcgccg gccagccgac ggccgggagg gacccgacgc gttcacgcgc cacggaagtg
     6721 gtccactcgc cctgggttcc gagcgggcgg tgaaaggaga gcccaccacc gtcggcgagg
     6781 tagtagtcac cgtaatcgcg tccccactcc tgcgtgttga tcgagttgaa gaggggtgag
     6841 atcaccggat ggtcggagac gtcgcggact tcccgatacc cggagagcgt gaagatcccg
     6901 accgggctgg tccacaccgc gtcccctcgt cctttgaccc ggcggtcccc gaagccgtaa
     6961 ctcgagcgcg cccggacctc gatccccgtt ccgccgacgc gctgagtcag ccccgccccg
     7021 gcggtcagcc cctcgacgcg attgacgtgc agaagatccg agagcgaacg aacacccaga
     7081 cgcgtcggcg acaggccggt catgacgtgg gtgccggcga tccgcgccgc ttccgcgcgg
     7141 aggcgctcga ggtcgtcggc gcgggccggc gtctcgacgc ttcccatcgc ctgcgccagc
     7201 gtgttcggcc agggaaagga atcaagctcc gctggcggaa ccgatgagat ctcctccccg
     7261 gcgaaccagg tgtcggccaa cccgagattg aacgcgtagt caccgatctc ccagcgcccc
     7321 cggatgatcc cgcgggccga gaaatccagc cagttgaccc gccgccggat ctcgatttcc
     7381 tgacgatagg ggagccagaa ccggccatcc cagagcgcgt cgtcgagtac gacgctcacg
     7441 tcctcgagct cgggatcgcg gtacgcggcc ggggtaaaat cgaacgccat gcgcaccagg
     7501 gcggccgtgg acacgtccag atagagcgtg ccgacgatcc gcggctggct gaactccttg
     7561 ggccgtacgt ggaccgcgac gactctgacg gtccgtcccg gcatggagac aagctgcgtg
     7621 tcgccgaggg cgtagtcgta gtcgagctgg gctccggggg acaggggatg gggcacgtcc
     7681 tgcacctcgt ccccttcgcc caggcggatg gcccggccga agttgttctg gatgatgccg
     7741 agatggtcgc ggtggtagtt gatgtccgtg ggaagctgcg ccgcattgcg ccacccgacg
     7801 atccgctgct tgctggcgcc cggggccttc cagtacaatt ccagttcgag ctgatcggtc
     7861 ttgatcaacc gcggtgcgtc gttcagcccg ttcccaaaag ccccgagaaa gaacacgaac
     7921 ccgtgcgcat gcgccttgta gtcgtggaga gcggtatcgg cagtggtggc gccgcggcgc
     7981 tggatcccgc ggctgaccag cgcgagggcg ggtggggcgt tccagtcctg ggcggcgagc
     8041 cggagggggg cccccgccat cgccaagagc agcagggcgc gcatcgccgg gtaatgtacg
     8101 cgctccttct gggcctcgcg gcgtgccata acagcgccgc tcccctcaac gtcatccacc
     8161 gcaaagacct cctggcgctc gcgcgctccg actcggccga tccgatcgac cggaccctcg
     8221 tcttccagaa caacaagacc gtttcaattt cgtggctgaa cagcgatacc ctggggtcga
     8281 cattcgcgac cttccatttc tacttgcaga gcgttcgttc gcggaacgat acgattctcg
     8341 cggacacgtc caccgtgact ctcaacgcat cggctactcc gggggtgttc ggattcactc
     8401 tgctgccgac atcggtcgtc ttcaacatcg ccggctcacc gaccatcgac gtgtcgtatg
     8461 cgcggtacgc cgacttcagc gtcatcgatt cggcgccttc caagtacccc aatccagccg
     8521 cgttcgccca ggccctggct ctctactatg agtacgggcc cgatcagtgg cgggccctga
     8581 atacgggcaa cctgtccaac gtggcttcgt ccgcactgtc gcagcccggt acgtacctcc
     8641 tcgccgcgcc gaaatgagtg atgcccgctc cgatttcctg acccgcgcgg cggcgggcgg
     8701 gatggtgccg ttggtgcgcg aggtgctcct cgacgccgac acgccggtgg ccgcgtacgc
     8761 gaagatcgcg gccggtccct tcgggtttct gctcgagtcc ctcgtaggcg gcgaacggtg
     8821 ggcccggtac acgttcctgg gcaccgagcc acgggaggtg tggcggtatc gcggccgctc
     8881 cgtcgaacgc tggaccccga aagacggctg gaccgcgcag ggacaggtcg aagcccccct
     8941 cgagcatctc gccgggctcc tccgccacgt cccagccgtc gaggcgcccg gccttccccg
     9001 gttcacgggg ggtgcggtcg gatacctggg ctacgagctg attcgcagcg tggagcgcct
     9061 gccgcacccg ccccaggata ccctgggcct ggcggacgcc gtcctcatga tcaccgacac
     9121 ggtggtcatt ctcgacaacc tctacggccg cgcctccatc gtggccaccg tcgaggtccc
     9181 ggcgggcgcg cccgcgcgcg aacgggaacg gctggccgac gaggccgagc gccgcgtgaa
     9241 cgccctggtc gcgcgtctcg aacggccgca cacccttccg cccctgcgca tgccggctca
     9301 ccccacgccg gtgccgacca cttcgcgcta cccggtggag cgtttcgagg cggacgtggg
     9361 ccgcatcctc gaatacatca aggcgggaga cgcgttccag gtcgtcctgt cacgcgcgca
     9421 ggaagcggcg ggtccggtcg atgcgctgcg cctgtaccga ttcctccgcg ccctcaatcc
     9481 ggcaccttac ctcttcttcc tgcagctcga cgactgcgcg ctggtggggt cgtctccgga
     9541 aacgctggta cgggtcgagg gtggagaggt cacggtgcgc ccgatcgccg gcaccgcccc
     9601 gcggggcgct tcgccgccgg aggatgagcg gctgggcggc gcgctcgcgg acgatccgaa
     9661 agaacgggcc gagcacctca tgctggtgga tctgtcgcgg aacgacgtgg gccgggtggc
     9721 ggcctacggc accgtgcggg tgaccaacct catggttctg gagcggtact cgcacgtgca
     9781 gcatctggtg agcgaggtgc gcggcaagct gcgcgccggc tacgatgccc tcgacgcgct
     9841 gcgggcctgc tttccccacg gcaccgtcac cggcgcgccc aaagtacggg cgatggagat
     9901 catcgacgag ctcgaaccgg aggctcgcgg tccctatgcg ggtgcggtcg gctacatcgg
     9961 gtggggtgcc aagagcctcg atactgcgat cgccatccgg gccgcgctgg tcctgaaaga
    10021 ccgggtggtg gtgcgggcgg gggccggcat cgtggccgac tccgatccgg gacgcgaatt
    10081 cgcggagacc gaacataagg cccaggcggt gctgaaggcc ctcgccctcg cgcgggccca
    10141 aggcccggga tagtttcccg ctcgtgttca agctgctcag catctcgggc gaacagtcca
    10201 tcgagctggc cgcggggaaa acgctcgtgg tcgggcgggc cgtcacctcc gacgtgccga
    10261 tttacgaccc cacgatctcc cgccgtcacg ccgaaatcac gctcaccgac gccggggtcc
    10321 ggatcaagga cctgggctcg tcgaacggca cgttcctgaa cggcgccaag gtgacggacg
    10381 gggacgccgc cgagaacgac gtggtgacct tcggcaaggt cgcgttcaag gtggttccca
    10441 tcacgccccc cgcgcgcgcg gccgtgacca cgcccgcccc gccgacgtcg aacaaggaga
    10501 ccaagggatc gggcgttccc ggcggcacca tcgtccgggc cttgcccatg tccccctccg
    10561 gcaacgttcc cgccatcaag gaaatcgggc cgtcgggggg ccacctcaag gtcgacgcga
    10621 aggatctgta cgaacggcag tcgcagaagc tgaaactgct gctcgacatt tcgcaggagc
    10681 tgtccaagca acaggacctg gaccgggtgc tcgagaaggt ggtggacctc accttccagg
    10741 tcatgagcgt ggatcgcgtg tccattctgc tcctcgacac gaagaccaac gagctcgtcc
    10801 cgcggatctc gcggtcccgc gccggagacg cgcaggccgc gaagatggtc ccgcaatcca
    10861 tcgcccgcaa agtggtggac gaccgcgtgg ccattctctc cgacaacacg gcggcggacg
    10921 accggttcaa gggcaagtcc atcgtgatcc agagcgtgcg cagcgccatg tgcacgcccc
    10981 tgctctcgaa cgaccagaag gtcctcggca tcctgtacgt ggacaatcag accgccattc
    11041 actcgttcca ggacgaagac ctcgacttcc tgatggcctt cggcgggctg accgcgttct
    11101 ccatcgagcg ctcgcagctg gccgaccgcc tccgccgcga agccatggtc ctctcgaact
    11161 tccagcggta cttcgccccg aacgtcgcgg cgcagatcgt ccagcaggag ggatcggtgc
    11221 agctgggcgg ggacaagcgg cccgtcgtga tcttcttctc ggatatccgc gggttcacgc
    11281 cgatgtcgga aaccatgagc ccggacgacg tcgcccatct gctcaccgaa tacttcaccg
    11341 agatggtgga tctcgtcttc gagcactcag gatcgctcga caagttcatg ggcgacgcca
    11401 tcatggcgct ctggggcgcg cccatcgcgc acgaagacga cgccgaccgc gccatgaagt
    11461 gcgccatgga ccagctggcc gcgctcgaaa agatgaacgt gaaatggaag gagaagggcc
    11521 gccccgacat caagatcggg atcggcatca acttcggtga ggtgttcgcc ggaaacttcg
    11581 gctccgaccg ccgcctcgag tacaccgtga tcggtgacgc cgtcaacacc gcgagccggc
    11641 tctgctccaa cgcgggcgcc aacgagatcc tgatctcgga gacgttctac aaagccctga
    11701 agcacccccc caaggtcgaa gccctcgaac cgatgcaggt gaagggcaag acgaagaagg
    11761 tcccggtcta ccgggtgaag cggtaaggct ttccgaagcg ctccagcagg tcgagcacca
    11821 gccgcagcgg cagtcccatc accccgaaaa agtccccgcg aatgccgtcg atcagcgcgg
    11881 cgcccttccc ctggatggcg tacgcgccgg ccttgtccat cggctcacca gtggcgacgt
    11941 agctcgcgat ctgttccctc gagagacgcc gaaaggtgac gtccgaaacg tcgagtgcgg
    12001 tctccacccg ctcgccctgc gccagtgcca cgcccgtgta gacccggtgg gtgcggcccg
    12061 ccagtcgccc cagcatctcg acggcttcac ccaccgtggc cggcttcgcg aacagctcgt
    12121 cacccaggat gacggtggtg tccgccgcga gtaccagcgg ccgggggtcg cgaccggaca
    12181 cggcgcgcgc cttgtcgcgg gccagccgga cgacgtagtc ggcgggggct tctcccgccc
    12241 ggtgggcttc gtcgacatcg ggggggacga cgcgatgctc gatcccgagc atttcgagga
    12301 gctgccgccg ccggggcgag gccgaggcca gcacgaggac gggtgtgtcg gtcatttctc
    12361 gaggatcaac gtgacggggc cgtcgttcac cagctcgacg tccatcatcg cttgaaactg
    12421 gccggtcgcg acggggatgg ccccggcgtg ctcccgcaga acggccacga agcggtcgta
    12481 gagagcgtgc gcctgctcag ggggcgcggc cccgatgaac gaaggccggc ggcccttgct
    12541 ggcgtcgccg tacagggtga actgggagac tacgaggacg ccgccgttca cgtcggcgag
    12601 cgcaaggttc atcttgtctt cggcgtcgga gaacgcgcgg aggccgagga tcttgtcggc
    12661 catccatttg agcgtctcgt cggtgtcgcc gggcgcaaac ccgaccagcg cgacgaaccc
    12721 ccgccccacg gtccccacgg tctcccccgc cacgcggacc gcgccacggg agacgcgctg
    12781 gaggacgacc ctcactcgac cgtgacgctc ttcgcgaggt tcctcggctg atcgacgttg
    12841 cagccgcgcc ggacggcgat gtagtaggcg aggagctgca acggcagcac cgagaggatg
    12901 ggcgtaaaga tgtccagcgt ctcggggatc tcgatcttgt ggtccgccag cctggcgacg
    12961 cccgtgtcac cttcggtgac gaccgcgatg acgcggccgc cccgcgcctt cacctcttcg
    13021 atgttgctca cgaccttctg atagacggcg tcacgcggcg cgacgaacac caccggcatc
    13081 agatcgtcga tcagcgcgat gggcccgtgc ttcatttccg ccgccgggta cccctcggcg
    13141 tggatgtacg agatctcctt gagcttcagt gccccctcca gcgcgaccgg gaaattgtag
    13201 ccgcggccta ggtagaggac gttctgcgtg cgctggaggc gttcggcgat ctgctccacc
    13261 tgggccgcct tcgccacgac cttctccacc agctccggga gcttggccag cgccttgacc
    13321 acctcgcgcc cctgcaggat ggagagcgcg cgcaggcgtc ccagcttcag ggtaagcaag
    13381 gccagcgcgg cgatctgact ggtgaacgcc ttcgtgctcg cgacgccgat ttcagggccc
    13441 gcgtggaggt aaatgccgcc gtcgacctcg cgggcgatgg tggagcccac gacgttgacg
    13501 agcccgatgg tccgtgcccc gcgccgcttg gcttcgcgga gagccgccag cgtgtcggca
    13561 gtctcgccgg actgcgaaat gccgatcacc agggtgcgct cgtccacgat cgggttgcgg
    13621 tagcggaact cggacgcgta ctccacctcc gtcggaatgc gggccagctc ctcgatcatg
    13681 tattcgccga tcagggccga atgccagctg gtgccgcacg ccgtgatcac gatgcgattg
    13741 atgcggagca ggtcttcatc cgagatgttg aggccgccca gccggacgtc cccgtcatcc
    13801 tcgagcagac gtccgcggag cgtgttccgg agactctccg gctgctccat gatttccttg
    13861 agcatgaagt gcgcatagcc cccccgctcg acggtagcga ggtcccactc gacgtgcgtg
    13921 acggtctttt ccttctccac cgtgtggtgg tccaggatcc ggtattcggt gggccggatg
    13981 accgcgatct cgccgtcatc caggtagacc accgagcgcg tgtgcgagag gaccgccgac
    14041 gcgtccgagg cgatgaagtt ctcgcccgag cccacgccca cgaggagcgg cgagcccttc
    14101 cgggccgcga cgatcgtgtc gggctcacgg cccgagacca ccgcgatgcc gtaggtgcct
    14161 tccacctcgc gcagcgcgcc cgcgaccgac tcggtgaggg gcgcgccctt ggtgtgcagc
    14221 tcttcgatca ggtgcgccag gacttcggta tcggtctcgg agcggaacac gtgcccgcgc
    14281 ttggtcagcg cggtgcggag ggcgccggcg ttctcgatga tcccgttatg gaccacggcg
    14341 atggtgccgc cgcaatc
//
LOCUS       DASUBP010000086         2471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_983187, whole genome shotgun sequence.
ACCESSION   DASUBP010000086 DASUBP010000000
VERSION     DASUBP010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_983187"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1173)
                     /locus_tag="VFU75_03420"
     CDS             complement(<1..1173)
                     /locus_tag="VFU75_03420"
                     /inference="COORDINATES: protein motif:HMM:NF018899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HEU5465910.1"
                     /translation="MTLAAPLIPLAAQNDQRVVRGLSFEGNKSIDSYTLSSAIATTPS
                     AWLARAWWVRWLGLGEKRYLDEIEFRRDVVRLLLLYRQSGYMRVQIDTVVKRTPDAAW
                     IDFRITEGPPVRVTRLTISGTEGILDTTALRKDLPLKVGAPFNRFLFQASADTIAARL
                     QNMGHPAAEVLRNFDSNADSLTATVELDVEPGAFAHIGNVQVRGLRRVDTSTVLHALP
                     FRTTDMYRQDQLYESQRNLYSLGLFTSVTVGPADSLEPGDSIVNIAVQVAEGPRHAIR
                     GGVGYGSVDCFRAQAGWSALDFFGGGRSLDLTGSVSKIGVGYPTNAGFQKNVCSYLKS
                     DSTNSDTLNYSLSATLSQPSFLSPRHTASLTVLGERRSEYRVYTRQDVGANVGVVFN"
     gene            1278..>2471
                     /gene="dacB"
                     /locus_tag="VFU75_03425"
     CDS             1278..>2471
                     /gene="dacB"
                     /locus_tag="VFU75_03425"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HEU5465911.1"
                     /translation="MRIQTLLFAISFPVSILGSQTPAPRTKPLRGSLERRINTLLDEP
                     PFNRAQWGVYVIDDQGKVRYQRDGDRYRVPASNTKLVVTSTATVLLPPDYRVRTSLYT
                     NGTLTGGVLSGDLVLYGRGDPTFSERCFGIDTLAPGACDSAFTAMNAIAESLWAKGVR
                     RINGKLVGDGSYFEPTLTAPGWNAWDLNWWYAAPVSGLGFHDNSVDFHITPGAAVDAP
                     PTVTWSPDVGLITFENRARTVPADSGSSIGDEFYRTPGTLNIWANGTVALDRKPWIES
                     FALPDPNLYAARALEVALERRGISVLGGAVSTTDSMEYRSVRANPALVDYLGRPLPDI
                     IFPILNTSQNWFAEMLLKIVGHEFRGVGSWDAGLDVERRFLIDSVGIDSTAFSIEDGS
                     GLSAGN"
BASE COUNT          434 a          800 c          805 g          432 t
ORIGIN      
        1 gttaaagacg accccgacgt tggcacccac atcctggcgc gtgtagactc tgtattccga
       61 tcgccgctcg ccgagcaccg tcaatgacgc cgtgtgccgg ggcgacagga atgagggctg
      121 cgacagggtg gccgacagcg agtagttcag ggtgtcagaa ttcgtcgagt cgctcttcag
      181 ataactgcac acgttcttct gaaacccggc gttcgtgggg tatcccacgc cgatcttcga
      241 cacactcccg gtgagatcga gggaccggcc cccgccaaag aagtcgagcg ccgaccagcc
      301 ggcctgcgca cggaagcaat ccaccgaccc gtacccgacg ccgccccgaa tggcatgccg
      361 cggcccttcg gccacctgga cggcgatgtt cacgatgctg tcgccgggtt cgagcgaatc
      421 ggcggggccg accgtgaccg aggtgaagag gccgaggctg tagagattcc gctggctctc
      481 gtaaagctgg tcctgccggt acatgtccgt ggtgcgaaac ggcagggcgt ggaggacggt
      541 gctggtgtcc acccgccgga ggccgcggac ttgaacgttg ccgatgtgcg cgaaggcgcc
      601 cggctcgacg tcgagctcca ccgtggcggt cagagaatcg gcgttggaat cgaagttccg
      661 gagtacttcc gcggccgggt gtcccatgtt ctggagccgc gcggcgatgg tgtcggccga
      721 cgcctggaac aggaagcgat tgaatggcgc acccaccttg agcggcagat ccttccggag
      781 ggccgtcgtg tccagaatgc cctcggtgcc gctgatggtc agccgggtca ctcgcaccgg
      841 aggaccctcg gtgatgcgga agtcgatcca ggccgcgtcg ggggtgcgtt tcaccaccgt
      901 gtcgatctgc acccgcatgt atccgctttg gcggtacaag agcagcagac gaacgacgtc
      961 ccgccggaat tcgatctcgt cgaggtatcg cttctctccc aacccgagcc agcgcaccca
     1021 ccaggcccgg gccagccagg cggatggcgt cgtggcgatg gccgagctga gggtgtagct
     1081 gtcgatcgac ttgttgcctt cgaaggacag cccacggacg acccgctggt cgttctgagc
     1141 agcgagagga atgagagggg ccgcgagggt cactgcggcg aacagagcca tcgtacctgg
     1201 gcgcaattga cgtgactgta taagtcctgt atgttgggca acttccgtcg cactgtcaac
     1261 cttttcgaca ccactccatg cgcattcaga ccctcctgtt cgccatctcg ttccctgttt
     1321 cgatcctcgg ctcccaaact ccggccccgc gcaccaagcc gctccggggg tcgctcgagc
     1381 gtcggatcaa cacgctcctc gacgaaccgc cgttcaaccg ggcgcaatgg ggtgtgtacg
     1441 tcatcgacga ccaggggaag gtccggtacc agcgggacgg cgaccggtat cgggtgcccg
     1501 cgagcaacac caaactcgtc gtgacgtcga cggccacggt gctcctccct cccgactacc
     1561 gggtccgcac cagcctgtac accaatggca cgctcacggg cggcgtactg tccggcgacc
     1621 tcgtgctcta cggccgcgga gacccgacgt tttccgagcg ctgcttcggc atcgacaccc
     1681 tcgcccccgg cgcctgcgac tcggccttca ccgcgatgaa cgcgatcgct gaatcactct
     1741 gggcaaaagg cgtgcgacgg atcaacggca agttggtggg agacggatcg tacttcgagc
     1801 cgaccctgac ggcgcccggc tggaacgcgt gggacctgaa ctggtggtac gcggcaccgg
     1861 tgtcggggct cggattccac gacaacagcg tggattttca catcacgccg ggtgcggcag
     1921 tcgacgcccc gccgaccgtt acgtggtcac ccgacgtggg attgatcacg ttcgagaatc
     1981 gcgcccgcac cgtgccggcg gactcgggat cgtccatcgg agacgagttc taccggacac
     2041 cagggaccct gaacatctgg gcgaacggca ccgtggcgtt ggaccggaag ccctggatcg
     2101 aaagcttcgc ccttcccgac ccgaatctct acgccgcccg ggccctcgaa gtcgccctgg
     2161 agcgccgcgg catctcggtg ctgggcgggg cggtcagcac gacggattcg atggagtaca
     2221 ggagtgtccg ggcgaacccg gcactcgtgg actatctggg tcgccccctc cccgacatca
     2281 tcttccccat cctcaatacc agtcagaact ggtttgccga gatgctcctg aagatcgtgg
     2341 ggcacgagtt ccggggagtg ggatcgtggg atgccgggct cgacgtggag cggcgtttcc
     2401 tgatcgattc agtgggcatc gactcgacgg cgttttcgat cgaggacggt tcgggacttt
     2461 cggccgggaa c
//
LOCUS       DASUBP010000087         2103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_547279, whole genome shotgun sequence.
ACCESSION   DASUBP010000087 DASUBP010000000
VERSION     DASUBP010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_547279"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1029)
                     /locus_tag="VFU75_03430"
     CDS             complement(<1..1029)
                     /locus_tag="VFU75_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465912.1"
                     /translation="MCSRVLALVLGCSVPAVGAVRAQVLDTVHLHHKADSLLALWREA
                     NALGVVQQAVRAVRHQKATQVTRATAVARGERPVRAGALMVIADYPDSIPLQGAANHA
                     WDVLSTTYGSQAAVLAAQPIRLAVVFGNREGVASPTSRRVPRKVTEDELERMLLGMTG
                     QPKVDSRFSRWLGNSVHPVLDTIADRAGAYEQLVTAGSTAATSCFKGGIEGCVAVLQI
                     PEDSLFYLTVYDAAQRRGTVAATRSPDMVDPAEHARYSSCVDDGRDSACIEFLRGIPL
                     GQIPQPVNFEVRNLLVSTALSLGGQGAYDRLMADSTAPVSTRLERAANAPLEKIVGTW
                     RTNVMAARP"
     gene            complement(1022..1675)
                     /locus_tag="VFU75_03435"
     CDS             complement(1022..1675)
                     /locus_tag="VFU75_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465913.1"
                     /translation="MFNAILYQQWKWTRLIVALGTVVAFTIPIFSLSSSGADLDHRAA
                     AVFLGHVQGWGVFYPTLAGTLGVLVAIGLWAPDHRGRHVYSLALPIPRWRYVALRYLA
                     GLTLLAAPVAALALGAVIASATASLPVGLHSYPIGLAFRFTLAILVAFTLFFAVSSGT
                     ARTAATILGTVAGLVVVQVLLSATGSQLNILMPIANALLTAPGPFAIFTGRWMLIDV"
     gene            complement(1668..>2103)
                     /locus_tag="VFU75_03440"
     CDS             complement(1668..>2103)
                     /locus_tag="VFU75_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465914.1"
                     /translation="DPVVRRDMLSAVLDYVSQHDATVFISSHLIHELERICDWVGVMD
                     NGRLVADVEMERFKTGMKRLKVSHGPANTSALPFRVLTRTPVGSNGSEMWLVRDWSPE
                     MAPSVAAAGGELQQIIDLDLEDGFVELLGSFRVPKTTEVTNV"
BASE COUNT          386 a          693 c          709 g          315 t
ORIGIN      
        1 ggggcgggcg gccatgacgt tcgtcctcca cgtcccgacg attttctcca gcggggcatt
       61 ggccgcccgc tccagccggg tcgacaccgg cgccgtcgaa tccgccatga gccggtcgta
      121 tgcgccctgc ccgccaagtg aaagcgccgt cgacaccagg agattccgta cctcgaagtt
      181 caccggctga gggatttgcc ccagcggaat gccgcgaaga aactcgatgc acgccgagtc
      241 cctgccgtca tcgacgcagc tcgaatacct cgcatgctct gccgggtcga ccatgtcggg
      301 actgcgggtt gccgcgacgg tgccccggcg ctgcgcggca tcgtagaccg tcagataaaa
      361 cagggagtct tccgggattt gcagaaccgc gacgcatccc tcgatgccgc ccttgaagca
      421 gctcgtcgcg gccgtcgatc ccgcggtcac gagctgctcg tatgcccccg cgcggtcagc
      481 gatggtgtcg agcaccggat gcaccgaatt ccctagccaa cgcgagaatc gcgagtcgac
      541 cttcggctgt ccggtcattc ccaggagcat ccgctcgagc tcatcctcgg tgaccttccg
      601 cggcactcgg cgcgatgtgg gtgacgctac gccctcccgg tttccgaaga ccacggccag
      661 gcggatcggc tgtgcggcga gtacggcagc ctgactccca taagttgtgg aaaggacgtc
      721 ccaggcgtgg ttggcggcac cctgcaacgg gatggagtcg gggtagtcgg cgatgaccat
      781 cagcgcgccg gcgcggaccg ggcgctcacc acgggccacg gccgtggcgc gcgtgacttg
      841 agtggccttc tgatgccgga cggcgcgcac ggcctgctgc accacgccga gtgcgttcgc
      901 ctcccgccag agagcgagca gggaatccgc tttgtgatgc aggtggaccg tatcgagaac
      961 ctgcgcccgc accgccccca cagcgggaac gctgcagccg aggaccagtg ccagcacccg
     1021 gctacacatc gatcaacatc cagcggcccg tgaagatcgc aaatggcccg ggagcggtga
     1081 gcaacgcgtt tgcgatcggc atgaggatgt tcaactggct gccagttgcc gagagcagga
     1141 cctgtacgac gacgagccct gcgacggtcc cgaggatggt cgccgccgta cgcgcggtcc
     1201 ccgacgaaac ggcgaagaac agggtgaacg cgaccagaat ggcgagggtg aatcggaagg
     1261 ccaggccgat gggatacgag tgcagaccca ccgggaggga tgccgtggca ctggcgatga
     1321 cggcgccgag tgccagggcg gcgaccggtg cggcgagcag cgtgagtccg gcgagatatc
     1381 ggagcgcgac gtatcgccaa cggggaatgg gaagggcgag cgaatagacg tgccggcccc
     1441 ggtggtcagg cgcccagagc ccgatcgcga cgagtacgcc gagcgtgccg gcgagggtcg
     1501 ggtagaacac cccccacccc tggacatgtc ccaggaacac cgccgccgcc ctgtggtcca
     1561 ggtccgcgcc ggagctggac agcgagaaga tcgggatcgt gaacgcgacc accgttccca
     1621 aggcgacgat cagccgggtc cacttccact gctgatagag gatcgcatta aacattggtc
     1681 acctccgtcg tcttcgggac gcggaaggag cccaggagct ctacgaatcc gtcttcgaga
     1741 tcgaggtcga tgatctgctg gagctcaccg cccgccgccg cgaccgatgg cgccatctcc
     1801 ggggaccagt cccgcaccag ccacatctcg gagccgttgc tcccgaccgg agtccgggtg
     1861 aggacgcgga agggcagggc cgaggtgttc gcgggaccgt ggctcacctt cagtcgtttc
     1921 atcccggtct tgaagcgctc catctcgacg tcagccacga ggcggccgtt gtccatcacg
     1981 ccgacccaat cgcagatgcg ctcgagctcg tgaatgagat gggaggagat gaagaccgtg
     2041 gcatcgtgct gggacacgta gtcgagcacc gcgctcagca tgtcgcgccg caccaccggg
     2101 tcg
//
LOCUS       DASUBP010000088         2776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1051642, whole genome shotgun sequence.
ACCESSION   DASUBP010000088 DASUBP010000000
VERSION     DASUBP010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2776
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051642"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..556
                     /locus_tag="VFU75_03445"
     CDS             <1..556
                     /locus_tag="VFU75_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5465915.1"
                     /translation="HHRLQEMTISLAVSDVLVARAKTGDLEALEGLYRAFETPVYNLA
                     RRILKNAEDAEDVLQETFLEVVKSIGTYRGDGHLWGWVRQIASSKALMKIRRENVRAA
                     DEFDETTAAGSPAPLVGVNLDLERALEHLSETGRAVVWMHDVEGYTHEEIAEMMGKTV
                     SFSKSQLARSHARLRKLLAAEPGV"
     gene            557..1126
                     /locus_tag="VFU75_03450"
     CDS             557..1126
                     /locus_tag="VFU75_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465916.1"
                     /translation="MTHCTMDDLLALKANEGSVWARKHLEACDVCRGELEALYQRVAQ
                     LKALPARSPARDRWSEIRASVVAARAKRHRRWATWSMAAAAGLAAVLVFSPFSPPAAK
                     ADLAQVKAQSAALESRLQAPEAEGRVVSGRDAALIAQLEDQIEQIDGQLANQRTEQVD
                     AQLQLWQQRVNLMNQLVQVRVVRASYVGL"
     gene            1147..2157
                     /locus_tag="VFU75_03455"
     CDS             1147..2157
                     /locus_tag="VFU75_03455"
                     /inference="COORDINATES: protein motif:HMM:NF024578.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU5465917.1"
                     /translation="MNRKLLLLCTTCVLPGVLAAQGQPKPRRPEAARPGVMTYSFSGN
                     HARIGVVVNRTADADSDKIGARIAAVTPAGPAAKAGILAGDVITKFNGVSLANVRSED
                     SDESGPGNKLIELAQALDPGDTVQVEYRRGSDTKKVTLVAEDVSYATNGTFNMMPDRN
                     FSWNVMPRVDVEPRLAMPFGDMMKMDGWGSGEGMGMTLCFGDAWCNLELVSLNSDLGD
                     YFGTHDGVLVVKASEDSLLPLKSGDVIVSIGDRKPTSPAQAMRILRSYDSGETVNIEI
                     MRHQKRQTISWKVPSPEERERRSRDRVRTNSKMRTEEGARMERLARPLMERYRHVGLT
                     VI"
     gene            2264..>2776
                     /locus_tag="VFU75_03460"
     CDS             2264..>2776
                     /locus_tag="VFU75_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465918.1"
                     /translation="MRSLFASIVFLALATRAFAQTDSALMGKAGYWKPVTQRLPSGDP
                     NDGPDLQPFVNNLNAVLAILRRAPVLAQPRGFVASPVVQRELGVPHRPLTGSVRVGVL
                     FFRKDDGGSVVPQDAGPDVAVSINDASCVWNGHDVAFVDSTGSIYYDAPQDSSPVRGV
                     PGFSTDSSCLV"
BASE COUNT          492 a          861 c          967 g          456 t
ORIGIN      
        1 gcatcatagg ttacaggaga tgaccatctc actagccgtt tctgacgtcc tggtagcccg
       61 cgccaagacc ggcgacctgg aagccctcga agggctctac cgagccttcg agaccccggt
      121 ctataacctg gcgcgccgga tactgaagaa cgcggaggac gccgaggacg tgctgcagga
      181 gacgttcctc gaagtggtga agagcatcgg cacgtaccgg ggcgacgggc atctctgggg
      241 ctgggtccgg cagatcgcgt cctccaaggc cctgatgaag atccgccgcg agaacgttcg
      301 cgccgcggac gagttcgacg agacgaccgc ggccggttcg ccggcaccgc tggtaggtgt
      361 caacctggat ctggagcgcg cgctcgaaca cctgagtgag acggggcgcg cggtggtgtg
      421 gatgcacgac gtggaaggct acacacacga agaaatcgcg gaaatgatgg gcaagacggt
      481 gagcttctcc aagtcccagc tggcccggtc gcacgcgcga ctgcggaagc tgctcgccgc
      541 cgagccgggg gtttgaatga cacactgcac gatggatgac ctgctggccc tgaaggccaa
      601 cgaggggtcg gtctgggccc ggaagcacct tgaggcctgc gacgtctgcc ggggggagct
      661 ggaggccctt taccagcggg tggcgcagct gaaagcgctg ccggcccggt ctccggcgcg
      721 ggatcggtgg tccgagatcc gggcgagcgt cgtcgcggcg agggccaagc gccaccgccg
      781 gtgggcgacg tggtcgatgg ccgccgcggc cggactcgcc gcagtcctgg tgttcagtcc
      841 gttctcccct ccagccgcca aggcggactt ggcgcaggtg aaggcgcagt cggcggctct
      901 cgagagccgc ctgcaagcgc ccgaagcgga agggcgggtg gtatcggggc gggacgccgc
      961 gctgatcgcc cagctcgagg atcagatcga gcagatcgac ggccagctgg ccaaccagcg
     1021 gaccgagcag gtggatgccc agctgcagct atggcagcag cgagtgaacc tgatgaacca
     1081 actggtccag gtccgagtcg tccgggcctc ttacgtgggg ctctaggctc cggaggaatg
     1141 tcgcacatga atcgcaagct gctccttctc tgcactacct gcgtcctgcc gggcgtactg
     1201 gcggctcagg gacagccgaa accgaggcgc cccgaggcgg cacgtcctgg agtcatgacg
     1261 tacagcttca gtggcaacca cgcccgcatc ggggtcgtgg tcaaccgcac tgccgacgcg
     1321 gattccgaca agatcggtgc ccgcattgcg gcggtgactc ccgccggccc ggcggcgaag
     1381 gcggggattc tggccggtga cgtgatcacg aagttcaacg gcgtttcgct cgccaacgtg
     1441 cggtccgagg attcggacga atcgggcccg ggaaacaaac tcatcgagct cgctcaggcc
     1501 ctcgatccgg gtgataccgt gcaggtggaa taccggcgcg gatcggatac caagaaggtg
     1561 acgctcgtcg ccgaagatgt gagctacgcc accaacggca ccttcaacat gatgccggac
     1621 cggaacttca gctggaacgt gatgcctcgg gtggatgtgg agccgcgcct cgccatgcct
     1681 ttcggagaca tgatgaagat ggacgggtgg ggctcgggtg aagggatggg gatgaccctc
     1741 tgcttcggcg acgcctggtg caacctcgag ctcgtgtccc tgaactcgga cctgggtgac
     1801 tacttcggga ctcacgacgg cgtgctggtg gtgaaggcct ccgaggattc cctcctgccg
     1861 ctcaagagtg gagacgtcat cgtttccatc ggcgaccgga agccgacttc gccggcccag
     1921 gccatgcgga ttctgcggtc gtacgactcc ggcgagacgg tcaatatcga gatcatgcgg
     1981 caccagaaac ggcagaccat cagctggaag gtgccgagcc cggaggaacg ggaacggaga
     2041 agccgggatc gggtacgcac taactccaag atgcggaccg aagagggggc ccggatggag
     2101 cggttggcgc ggcccctgat ggagcggtac cgccacgtgg gactgacagt catttaggcg
     2161 cagcacagca agacggaata cgggggtccc gttcgggcgg ggcccccgtt gcgtttctgg
     2221 tcattcggcg tcgatttccg ggtaccccag ccaggattct tccgtgcgca gtctcttcgc
     2281 gtcgatcgtc ttcctcgccc tggcgaccag ggctttcgcg cagaccgact ctgccctgat
     2341 ggggaaggcc ggctattgga agccggtgac gcagcgcctt ccgagcgggg atccgaacga
     2401 cggtcctgat cttcagccct tcgtgaacaa cctgaacgcc gtcctcgcca tcctcaggcg
     2461 cgctccagtc ctggcacagc cgcgggggtt cgtcgcctca ccggtcgtcc agcgggagct
     2521 gggggtgccg caccggccat tgaccgggtc tgtgcgtgtg ggagtgctgt tcttccggaa
     2581 ggacgacgga ggatcggtgg tgccgcagga tgccggtccc gacgtcgcgg tctcgatcaa
     2641 cgacgcctca tgcgtgtgga acggtcatga cgtcgccttc gtggacagca cgggctccat
     2701 ctactatgac gcccctcagg actcgagccc cgtgcgggga gtcccgggct tctccaccga
     2761 cagctcctgc ctggtc
//
LOCUS       DASUBP010000089         3174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_778973, whole genome shotgun sequence.
ACCESSION   DASUBP010000089 DASUBP010000000
VERSION     DASUBP010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_778973"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..744)
                     /locus_tag="VFU75_03465"
     CDS             complement(<1..744)
                     /locus_tag="VFU75_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU5465919.1"
                     /translation="MRALVLSGGSIKGAYEAGAILSIVEAGFCPDAVLGISTGALSAA
                     FLVNAIGLKTDRAPAPSHWGEAASEFVQFYRTRLSSPELVVREKSWLAIAWSLLWNHF
                     DGVLDMTPLHRLIRGTLKEDALRRARVQLRVGAVDMGSGQIKYVGADHKDIEAFVRAS
                     CAEPLVMPSVRIAGADYYDGGLRDLVPIGTAINLGATEIVAVVCQPRVLDREVFPRGH
                     AVAMANRLVDVVTHEIVVNDLRSVERVNQI"
     gene            782..2053
                     /locus_tag="VFU75_03470"
     CDS             782..2053
                     /locus_tag="VFU75_03470"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5465920.1"
                     /translation="MIVAVMPFANLTTDPANAYFGDTAAEAIGDTLAVAAKLRVCPHA
                     AALDWADRGGSPQEVADGLSASALLEGGARRSAELVHFEVRLRAPNGTSLWSTMVDRP
                     VNEVLAAQLEIVTGVLGALKVDVSGEEVARMGRPLTADFRAYDQYLRGKIFSRELLRR
                     SQEAALHHFGEAIRTDPKFARAFAESAVCHGMIYQYWDSSADMLKRADEASLKALELG
                     PDLPHAHMARGLALSLAAKYAEADQEFSVALRMRPDLLDANYFRARSARAQGNYSEAV
                     SWFEKTWALRPLDYSVPALLASAYVSLDRKDDALATRKKAVELVDKRLIRHPDDSRAL
                     YLGAACLSTLGDGTKARSWAKRALAMDPDDSAVLYNVACVYALLGLDDSAIDCLEQAI
                     KHGFRHWDWIEHDSDFDQVRGNERFKALKQE"
     gene            2197..>3174
                     /locus_tag="VFU75_03475"
     CDS             2197..>3174
                     /locus_tag="VFU75_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465921.1"
                     /translation="MGRAFGLSLLFGLIAAPAWAGTGHPASGSKAAPPSETALRAYVA
                     NYMNGAYATATPSFARQTGLACSSCHTHYPELTAMGRAFKLNGYVFRRGGDSISARSS
                     SGDQNLLLNLVTPLSFMLQTSYTVLRKAEPGTQNGVAFFPDQLSLFTGGEVTPHLGGF
                     LQVTFDPQSGQLGIDNADFRYATHLSLLGKNSTFGISLNNNPTVQDVWNSTPAWGFPY
                     GSSAAAPTPAAATMIDGTLGGQVAGLTAYTMWGSHLYLEGGAYRAAPIGASRPLDSTA
                     TGVLAGAAPYWRVAIPNTIDGKYLSLGAYGMSSRIYPAGVTGPTNRFTDV"
BASE COUNT          552 a         1067 c         1011 g          544 t
ORIGIN      
        1 gatctgattc acgcgctcca ccgagcgcaa gtcgttcacg acgatttcgt gggtgacgac
       61 atccaccagc cggttggcca tggcgacggc atggccgcgg gggaacacct cgcgatccag
      121 caccctcggc tggcagacca cagcgacgat ttccgtggcg ccaagattga tggcggtgcc
      181 gatcggtacg aggtcgcgca ggccgccgtc gtagtagtcc gctcccgcaa tgcgcacgga
      241 cggcatcacg agaggttcgg cacaactggc gcggacgaat gcctcgatgt ccttgtgatc
      301 cgcccccacg tacttgatct gtcccgaccc catgtccacc gcaccgaccc gaagctggac
      361 ccgcgcgcgc cggagcgcgt cctccttcag ggttccccgg atgagacgat ggaggggagt
      421 catgtccagc acgccgtcga agtgattcca gaggagactc caagcgatcg cgagccagga
      481 cttttccctg accacgagtt cgggtgacga caaccgggtg cggtagaact gcacgaactc
      541 gctggccgcc tcgccccagt gagacggggc cggcgcccgg tcggtcttca gtccgatcgc
      601 gttgacgagg aatgcggcgc tcaaagctcc cgtcgagatg cccagcaccg cgtcggggca
      661 gaaccccgcc tcgacgatgg agagaatcgc gcccgcttcg taggcgccct tgatcgaacc
      721 gcccgaaaga accagtgccc gcatggtttg ctcctgtgct gcctgaatct tatcctaggc
      781 catgatcgtc gccgtcatgc cctttgcgaa cctgaccacg gatcccgcca acgcctactt
      841 cggcgacacc gccgcggagg cgatcggcga caccttggct gtcgcagcca agctgcgcgt
      901 gtgtccccac gcggcggctc tcgactgggc cgaccgcggc gggtccccgc aggaagtggc
      961 cgacgggttg tccgccagcg ctctgctgga gggcggggcc aggcgctcgg cggagctcgt
     1021 gcatttcgag gtgcggctgc gcgctccgaa cgggacgtcg ctctggtcca cgatggtgga
     1081 tcgcccggtg aacgaggtac tggccgccca actcgagatc gtcaccggcg tgttgggcgc
     1141 gctgaaggtc gacgtatccg gtgaggaggt cgcccggatg ggccgtcctc tgaccgccga
     1201 cttccgggcc tacgaccagt acttgcgcgg gaagatcttc agccgcgagc tgttgcgccg
     1261 cagccaggaa gccgccctcc accattttgg cgaagcgatc cggaccgatc ccaagttcgc
     1321 gcgggctttc gccgagagcg ccgtttgcca cggcatgatc taccaatact gggactccag
     1381 cgccgacatg ctcaagcgtg cggacgaagc gagcctcaag gccttggagc tcggtcccga
     1441 tctgccgcac gcgcacatgg cccgtgggct cgcgctctcc ctcgccgcca agtacgccga
     1501 ggcggaccag gagttcagcg tggcgctgcg aatgcggccc gatctcctcg acgccaacta
     1561 tttccgggcc cgctcggcgc gcgcgcaggg gaactactcg gaagcggtaa gctggttcga
     1621 gaagacctgg gccttgagac ccctggacta ctccgtcccg gccctgctcg cgagcgcgta
     1681 cgtctcactc gatcgcaagg atgacgcgct cgcgacgaga aagaaggcag tcgagctcgt
     1741 cgacaagcgc ctgatccgtc accccgacga ctcccgggcg ctgtatctgg gagccgcctg
     1801 cctgtcgacc ctcggcgacg gcaccaaggc gcgctcatgg gccaagcggg cgctggcgat
     1861 ggaccccgac gactccgcgg tgctctacaa cgtcgcctgt gtgtacgccc tcctcggtct
     1921 cgacgattcg gcgatcgact gcctggaaca ggccatcaag cacggattcc ggcactggga
     1981 ctggatcgaa cacgactcgg atttcgatca ggtccgcggc aatgagcggt tcaaagcgtt
     2041 gaaacaggag taatgtcagg aaaccactcg tttgtgctat tctgaaacgt ttcagccgca
     2101 gtctgaaggc ccgttcatca aatcttcacg atccgcgtat ccgccctgtc gcagtctttc
     2161 ggccaccttc acgaaagcct ttggcaagga gcgtacatgg gtcgcgcatt cgggttgtca
     2221 ctcttgttcg ggctgatcgc cgccccggcg tgggcgggaa cggggcatcc ggcttccggg
     2281 tccaaggcgg caccaccgtc ggagaccgcg ctccgggcct atgtcgccaa ctacatgaat
     2341 ggcgcgtacg cgacggcgac gccgagcttc gctcggcaga ccggactcgc ctgtagctcc
     2401 tgccacaccc actatcccga gctcaccgcg atgggccggg cgttcaagct gaacggatac
     2461 gtcttccgcc gtggcggtga ctcgatctcg gcccgcagct cgtccggcga ccagaatctg
     2521 ctcctgaacc tcgtgacgcc gctctccttc atgctgcaga cctcgtatac cgtgctccgg
     2581 aaggcagagc cgggcacgca gaacggcgtg gcgttcttcc cggatcagct gagcctcttc
     2641 accggcggcg aagtgacgcc gcacctgggg ggattcctgc aagtgacgtt cgacccgcaa
     2701 agtggacagc tcgggatcga caacgcggac ttccggtacg cgacgcacct ctccttgctc
     2761 gggaagaact cgacgttcgg tatttcattg aacaacaatc cgaccgtgca ggacgtgtgg
     2821 aacagcacgc cggcgtgggg atttccgtac ggatcctcgg cggcggcccc gacgcccgcg
     2881 gcggcgacga tgatcgacgg caccctcggt ggccaagtgg cgggcctgac ggcgtacacc
     2941 atgtggggca gccatctcta tctcgaaggt ggagcgtacc gggcagcgcc gatcggggct
     3001 tcacggccac tcgattcgac ggcgaccggt gtgctggctg gagcagcgcc gtactggcgc
     3061 gtcgcgattc cgaacacgat cgacgggaag tacctgtcgc tcggcgcgta cgggatgtcg
     3121 tcacgcatct atcccgcagg cgtcacggga cccaccaatc gattcacgga tgtc
//
LOCUS       DASUBP010000090         2457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1051710, whole genome shotgun sequence.
ACCESSION   DASUBP010000090 DASUBP010000000
VERSION     DASUBP010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051710"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1651)
                     /gene="tkt"
                     /locus_tag="VFU75_03480"
     CDS             complement(<1..1651)
                     /gene="tkt"
                     /locus_tag="VFU75_03480"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEU5465922.1"
                     /translation="MTRSGSALDDLCINTIRTLSIDAVQHAASGHPGLPMGAAPMAYA
                     LWTRHLRHDPASPSWIDRDRFVLSAGHGSALLYSLLCLTGYGLTIEDLKQFRRWGSRT
                     PGHPERGVTPGVEVTTGPLGQGFGNAVGMAITERWLAATFNRPGHAIIDHHTYVLASD
                     GDFMEGVTSEAASLAADLHLNRLIVLYDANQITLSGTTNITFAEDVGARFRAYGWHVQ
                     EVDGMDVAAVDSALTAGRAERDRPSLIIARTHIGFGSPHKHDTFEAHGEPLGGEEVRL
                     TKRSYGWPEDAQFLVPEEAAREFAKVGVRGAALRAEWQGVMDAYRAADGELAADLDRA
                     LAGELAPGWDAALPQFTPADGDLATRDAGGRVITALAESLPNFIGGSADLDPSTRTMM
                     KGKGDFQSAEVPQEGQTPPTQGAAGGTWSYAGRNIHFGIREHAMTAILTGMAHHGGVV
                     PFGATFLTFSDYMRPSIRLAALSGAHVIYVWTHDSIALGEDGPTHQPVEQLASLRAMP
                     NLLVLRPADATETVEAWRVALRHPGGPVGLVLTRQKLPVLDR"
     gene            complement(1648..>2457)
                     /locus_tag="VFU75_03485"
     CDS             complement(1648..>2457)
                     /locus_tag="VFU75_03485"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HEU5465923.1"
                     /translation="GTDGWVSLEVSPRLANDTAGTVAAAMKLHARAQRPNLFIKIPGT
                     REGVPAIEETIFRGVPVNVTLLFSAAQYTAAAEAYWRGIERRIAAGLDARVDSVASIF
                     VSRWDVAVHDRVAPALRNRLGIAVARQTYRAYHALLASRRWLSLADAGARPQRLLWAS
                     TGTKDPQASDTLYVEALAAPDTIDTIPEKTLAAFAEHGRVDGVMSVDGGDAAEVLAAF
                     QRAGVDEAVLADQLQQDGTASFTNSWNDLLVRVAAKRVLLAGDLRSTERAR"
BASE COUNT          394 a          865 c          807 g          391 t
ORIGIN      
        1 tgcgatcgag caccggcagc ttctgccggg tgagcaccag gcccaccggc cccccgggat
       61 gacgcagcgc gacgcgccac gcttcgacgg tttcggtggc gtcggcgggc cgcaagacga
      121 ggagattcgg catcgcccgc aagctcgcga gctgctccac gggctggtgc gtgggaccgt
      181 cctctcccag cgcgatgctg tcgtgggtcc acacatagat cacgtgcgcg ccgctcaagg
      241 ccgccaggcg gatgctgggg cgcatatagt ccgaaaacgt caggaaggtc gcaccgaacg
      301 gcaccacacc gccgtgatgc gccatccccg tgagaatcgc ggtcatggcg tgttcgcgga
      361 tgccgaagtg aatgttccgc cctgcgtagc tccacgtccc acctgccgct ccctgtgtgg
      421 gaggcgtctg cccctcctgc ggcacctccg cgctctgaaa atcccccttc cccttcatca
      481 tagtgcgggt ggacggatcg agatccgccg atccaccgat gaagttcggc agggattccg
      541 cgagcgccgt gatcacccgt ccccccgcgt cccgcgtagc cagatccccg tccgcaggcg
      601 tgaactgagg aagcgccgcg tcccatcccg gagccagctc gcccgcgagc gcgcggtcca
      661 ggtcggccgc caactcgccg tccgccgccc gatacgcatc catgaccccc tgccactcgg
      721 cccggagagc agcgccacgt accccaacct tggcgaattc ccgcgcggcc tcctccggta
      781 ccaggaattg tgcgtcttcg ggccagccat agctcctctt ggtcaaccgg acttcttcgc
      841 cgccgagggg ctcaccatgg gcttcgaagg tgtcgtgctt gtgaggactg ccaaagccga
      901 tgtgggtgcg cgcgatgatc agtgagggcc ggtcgcgttc cgcgcggccc gcggtgagcg
      961 ccgaatcgac ggcggccacg tccatgccgt ccacctcctg tacgtgccac ccgtacgccc
     1021 ggaagcgggc cccgacgtcc tcggcgaacg tgatgttcgt cgtgccggac agcgtgatct
     1081 ggttcgcgtc atagaggacg atcaggcggt tcaggtgcag gtcggcggcg agcgacgccg
     1141 cttccgacgt gacgccttcc atgaagtcgc cgtcgctcgc cagaacgtac gtgtggtgat
     1201 cgatgatcgc gtggcccggc cggttgaacg tggcggcgag ccagcgttcg gtgatcgcca
     1261 tcccgacggc gttgccgaac ccctgtccga gtggcccggt cgtcacttcg actccggggg
     1321 tgactcctcg ctcggggtgt cctggagtcc ggctgcccca gcgccggaac tgcttgagat
     1381 cctcgatggt gagaccgtac cccgtgaggc acaggagact gtacagcagc gcactgccat
     1441 gcccggccga caggacgaac cggtcccggt caatccacga gggggaggcg gggtcatggc
     1501 ggaggtggcg agtccacaac gcgtaggcca tcggggcggc gcccatcggc aaccccgggt
     1561 gtccggaagc cgcatgctgg acggcgtcga tactcagggt gcggatggtg ttgatgcaga
     1621 gatcgtcgag ggcgctcccg gaccgggtca ccgtgcgcgc tcagtcgatc gcaaatcccc
     1681 tgccaacaac acccgcttgg ccgccacgcg aacgaggagg tcgttccacg agttggtgaa
     1741 ggacgccgta ccgtcctgct ggagttgatc cgccaagacc gcctcgtcca caccagcccg
     1801 ctggaacgcc gccagcacct ccgcggcgtc tcctccatca accgacatca cgccatccac
     1861 gcgtccgtgc tccgcgaacg ccgccagcgt cttctcaggg atcgtatcaa tggtatcggg
     1921 agcggccagg gcctcgacgt acagcgtgtc cgacgcctgg ggatctttgg tcccggtact
     1981 cgcccagagc agacgctgag gccgggcacc ggcgtcggcc agcgagagcc accggcgaga
     2041 tgccaggagg gcgtgatacg cccggtatgt ctgccgcgcc accgcgatgc cgaggcgatt
     2101 gcggagtgcg ggcgccaccc ggtcatgcac tgcgacgtcc cagcggctca caaagatcga
     2161 cgcgaccgaa tcgacccgcg catcgagtcc cgccgcgatg cgccgctcga tcccgcgcca
     2221 gtaggcctca gcagccgcgg tgtattgcgc cgccgagaag agcagcgtga cgttcaccgg
     2281 gacgccgcgg aagatcgtct cctcgatggc cggtacgccc tcccgcgttc ccggaatctt
     2341 gatgaaaaga ttgggacgtt gagcccgtgc gtggagtttc atggcggccg ccaccgtgcc
     2401 cgcggtgtcg ttcgcgagcc gcggcgacac ctcgagggac acccagccgt ccgttcc
//
LOCUS       DASUBP010000091         4853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_874372, whole genome shotgun sequence.
ACCESSION   DASUBP010000091 DASUBP010000000
VERSION     DASUBP010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4853
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874372"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1035)
                     /locus_tag="VFU75_03490"
     CDS             complement(<1..1035)
                     /locus_tag="VFU75_03490"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465924.1"
                     /translation="MEFVLALHSHLPYVLNHGRWPHGSDWLCEAAIDTYLPLLEHLRA
                     LKVEKTPAPVTIGFTPVLANQLASDDFRKEFETFMNQRETACKEAPASLAKTADSSLL
                     PVVEYWKERFSRLRRLYDEADRDVVGAFRALQDSGRLEIIGSAATHGYLPLLARDESI
                     QLQLAIGQSEHKRLFGTAPKGCWLPECAYRPGIEKHLADAGFRFFFVDAHLAHAGTPL
                     GVDYDIPLGAERFDSERHGTPHPVDAKIEHTPYRAYRVSKGRRAVSAFVRDPQSSRQV
                     WSRDLGYPGDAAYLEFHKIRWPGGLKFWRVTGPHVDLGAKTAYDPGAALGRATEHARH
                     FTELLGGISER"
     gene            complement(1035..2549)
                     /locus_tag="VFU75_03495"
     CDS             complement(1035..2549)
                     /locus_tag="VFU75_03495"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HEU5465925.1"
                     /translation="MTRVAPSVDARGARRRAEPRKRPKAPGVVHLTAEYWPLARTGGL
                     GEAVNGLATYQAGTGTRTAVVMPLYQMVRDKVHALERVGPSFLVTFGSRVEEAWLYRI
                     PSDSGPQVYLIEHPGYFDRAAIYGDGADYPDNARRFAFFSLAAIAALPQIAPDAKVLH
                     AHDWHTALAIVYLRTRYADKPFFRDLATVLSVHNAGFQGHYPPETVADVGLPAELFDW
                     RVFEWYGQMNVLKGGLVFADAAVTVSPTHARELLTPEGGFGLHDVFTGLGERLRGVLN
                     GIDAEKWNPESDPGVPTHFSSTNLSGKRACKRALQHAYGLTEDPDLPLFGMSARLVSQ
                     KGLDLVLGGTLLHRPDAQFIFLGAGERRYHDLLAAHADRFPDRVAVEFAFTDRLEHGL
                     LSGADLLLMPSLYEPCGLTQMRAQRYGAIPVARRVGGLQDSIEDGRTGFLFTEYTPEA
                     LEAGVERALTVYRDKRAWSAMVKRSMELPFGWGGAAERYQEIYRMALTHRAGAL"
     gene            complement(2546..4708)
                     /gene="glgP"
                     /locus_tag="VFU75_03500"
     CDS             complement(2546..4708)
                     /gene="glgP"
                     /locus_tag="VFU75_03500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HEU5465926.1"
                     /translation="MTGSPEGSTPPLSLPLPPRIAGLAALTRNLDWSWSQDARQLLRS
                     IDQTLWHLTRHNPIELLKRVDPARLAALAADADFLKRYDQVMEQEAHRTSGQGTWFRH
                     EHPELQGPAAYFCAEFAFHNSVPIYSGGLGILAGDHCKAASDLGVPLVGVGLLYTRGY
                     FDQRVRLDGWQEDADEHFDASLTPLERLPTVVQLRMGGREVSVGVWRLMVGRVPIYLL
                     DTDIEPNTPADRRLTHRLYVGEQHMRLSQEWILGVGGVRVLRALGINPAAWHSNEGHA
                     AFMLLERLRELTVEGVPLAEATRQVRANSIFTTHTPVPAGHDAFTSADVEANAGSIWE
                     EMGLDREAVLGLGHHPVHDHGLFHMAVLALRLSGRVNAVSRRHGEVSRGLWKDLWPDR
                     DAAQVPISHVTNGVHLETWMAGTVSALLDGHMKHGWLDRSGDGSWDAVLTLNDAALWS
                     THRDLKNNLLSAIREAARRRWVEQWKGEGQHLVGSGILLEERALTIGFARRFATYKRA
                     DLLFHDLDRLHRLLVNPWRPVQLVFSGKAHPADEPGKRILQRVYNLTRDPRFEGRIAF
                     IEDYEMHLAHRLVQGVDLWLNLPRVPLEASGTSGMKAALNGVPQLSTFDGWWAEAYNG
                     RNGWVIPPAPEGGDVDLADAADADQLYRLLESEVVPLFYARDEQGIPRGWVDRMKHAL
                     QVAGQGFTARRMVREYVEGFYVPAMSEGFVDLDPVAAG"
BASE COUNT          820 a         1628 c         1636 g          769 t
ORIGIN      
        1 gcgctcggaa atccccccca gcaactcggt gaagtggcgg gcatgctcgg tggcccggcc
       61 caatgccgcg ccggggtcgt acgcggtctt ggcgccgagg tcgacatggg gaccggtgac
      121 gcgccagaac ttgaggcccc cgggccaccg gatcttgtgg aactcgaggt aggccgcgtc
      181 acccgggtag ccgagatcgc ggctccagac ctggcgggag gattgcgggt cccggacgaa
      241 cgccgagacg gcccggcgcc ccttggacac gcgatacgcc cgatagggcg tgtgctcgat
      301 cttcgcatcg accgggtgcg gtgtgccgtg gcgctccgaa tcgaagcgtt cggcccccag
      361 cggaatgtcg tagtcgacgc cgaggggcgt gccggcgtgc gcgaggtgcg cgtcgacgaa
      421 gaagaaccga aagccggcgt cggccaggtg cttctctatt ccgggacgat aggcgcattc
      481 ggggagccag cagcccttgg gggcggtgcc aaagagccgc ttgtgctccg actgcccaat
      541 ggcgagctgg agctgaatgc tttcgtcacg cgcgagaagg gggagatatc cgtgggtcgc
      601 cgcggatccg atgatctcga gacgtcccga atcctgaagc gcacggaacg ctccgaccac
      661 gtcgcggtcg gcttcgtcgt agagccgccg caggcgtgaa aagcgctcct tccagtattc
      721 caccaccggc aggaggctgg agtcggcggt cttcgccaat gatgccggcg cctctttgca
      781 cgccgtctcc cgctggttca tgaaggtttc gaattccttt cggaagtcgt cactcgccag
      841 ctgattcgcg agcaccggcg tgaagccgat cgtcacgggc gccggcgtct tctctacttt
      901 gagggcccgc aagtgctcga ggagtgggag atacgtgtcg atcgccgctt cacagagcca
      961 atccgagccg tggggccagc gcccgtggtt gaggacgtac gggagatgac tatggagggc
     1021 cagcacgaac tccatcacag ggctcctgcg cggtgcgtca gcgccatccg gtagatctcc
     1081 tggtatcgtt ccgccgctcc accccatccg aacggcagct ccatcgaacg cttcaccatc
     1141 gcgctccacg cgcgcttgtc gcggtagacc gtcagcgccc gttccacccc cgcttcaagg
     1201 gcctccggcg tgtactcggt gaacagaaag ccggtgcggc cgtcctcgat gctgtcctgc
     1261 agtccgccca cccgccgcgc gacgggaatg gcgccatacc gctgggcgcg catttgcgtg
     1321 agtccgcacg gctcgtagag ggacggcatc agcagcaggt cggctcccga aagaaggcca
     1381 tgttcgagcc ggtcggtgaa cgcgaactct accgccacgc gatcggggaa ccggtcggca
     1441 tgtgccgcga gcaggtcgtg ataccgccgc tccccggcgc caaggaagat gaactgcgcg
     1501 tcggggcgat ggaggagcgt gccgccgagg acgagatcca ggcccttctg cgatacgagg
     1561 cgggcgctca tcccgaagag cgggaggtcg ggatcctcgg taaggccgta ggcgtgctgc
     1621 agcgccctct tgcaggcgcg cttcccgctc aggttcgtgg acgagaagtg cgtggggacg
     1681 ccgggatccg actcgggatt ccatttctcg gcgtcgatcc cgttcaagac gccgcgcagg
     1741 cgttctccca gaccggtgaa gacgtcatgc aggccgaatc caccctcagg ggtgaggagc
     1801 tcgcgggcgt gagtcgggga caccgtgact gccgcgtcgg cgaacacgag cccgcccttc
     1861 agcacgttca tctgcccgta ccactcgaac acgcgccagt cgaagagctc cgccgggaga
     1921 cccacgtcgg ccacggtctc gggcggatag tgtccctgga agcccgcgtt gtggacggag
     1981 agcacggtgg cgagatcccg aaagaagggc ttgtcggcat agcgggtccg gagatagacg
     2041 atcgccagtg ccgtgtgcca gtcgtgcgcg tggaggacct tcgcgtccgg cgcgatttgc
     2101 ggcaatgcgg caatcgcggc cagcgagaag aaggcgaagc ggcgcgcgtt gtccggatag
     2161 tcggcaccgt cgccgtagat ggcggcgcgg tcgaagtacc ccggatgctc gatgaggtag
     2221 acctgggggc ccgaatcact cgggatgcga tagagccatg cttcttccac gcgtgagccg
     2281 aaggtcacga gaaacgaggg cccgacgcgc tcgagggcgt ggaccttgtc ccgtaccatc
     2341 tgatagaggg gcatcacgac ggcggtccgg gtcccggtcc ccgcctggta ggtcgccagc
     2401 ccgttcaccg cctcacccag cccacccgtc cgcgccaggg gccagtactc ggcggtgaga
     2461 tgcaccacac ccggtgcctt cggccgcttc cgcggttccg cgcggcgtct cgcgccgcgt
     2521 gcgtccacgg agggggccac gcgggtcacc ccgccgccac cggatcgaga tcgacgaagc
     2581 cctcgctcat ggccgggacg tagaacccct ccacgtattc ccgaaccatc cggcgggccg
     2641 tgaatccctg gccggcgacc tgcagcgcgt gcttcatgcg atcgacccac cctcgcggaa
     2701 tgccctgttc gtcccgggcg tagaacaagg gtaccacctc cgactccagc aggcgataga
     2761 gctgatcggc gtcagcagcg tccgccaggt ccacgtcccc gccctccggc gccggcggaa
     2821 tgacccaccc gttgcgtccg ttatacgctt ccgcccacca cccatcgaac gtgctgagct
     2881 ggggcacgcc gttcagcgcc gccttcatcc cgctcgtgcc cgacgcctcg agagggaccc
     2941 gcgggaggtt gagccacaga tccacgccct gcaccagccg gtgggccaga tgcatctcgt
     3001 aatcttcgat gaacgcgatg cgtccctcga agcgcggatc ccgcgtcagg ttgtacaccc
     3061 gctgcagaat ccgcttcccg ggctcgtcgg ccgggtgcgc cttccccgag aacaccagct
     3121 gtaccggccg ccacgggttc acgagcaggc gatgcagccg gtcgagatcg tggaagagca
     3181 ggtcggcgcg cttgtaggtg gcgaaccggc gggcgaaccc gatggtcagc gcccgctcct
     3241 cgaggaggat accggagccg acgagatgct gcccttctcc cttccactgt tccacccagc
     3301 ggcgccgcgc ggcttcccgg atggccgaca gaagattgtt cttgaggtcg cggtgcgtgc
     3361 tccagagcgc ggcatcattc agcgtcagca ccgcatccca cgacccgtcg ccggaacggt
     3421 cgagccaccc gtgcttcatg tggccgtcaa gcagggcgct caccgtcccc gccatccacg
     3481 tctcgagatg cacgccgttg gtcacgtgag agatgggtac ctgcgccgcg tcacggtcgg
     3541 gccacagatc cttccacaat ccgcgcgaca cctcaccatg ccgccgtgac accgcgttga
     3601 cgcggccgga gagccggagt gcgagcacgg ccatgtgaaa cagcccgtgg tcatggacgg
     3661 gatgatgccc caatccgagc acggcctcac ggtccaggcc catctcttcc cagatcgagc
     3721 cggcgttggc ttcgacgtcg gccgaggtga aggcgtcgtg cccggcgggt acaggggtat
     3781 gcgtcgtgaa gatgctgttc gccctgactt gccgcgtagc ttcggccagc ggaacgccct
     3841 ccaccgtcag ctcgcggagc cgctcgagga gcatgaacgc ggcatgtccc tcgttcgaat
     3901 gccaggcggc cggattgatg ccgagggcgc gcaggacgcg cacgcccccc acgcccagga
     3961 tccattcctg tgacaggcgc atgtgctgct cgccgacgta gagccggtgc gtgagccggc
     4021 ggtcggccgg cgtattcggc tcgatgtcgg tatcgagcag gtagatcggc acccggccca
     4081 ccatcaaacg ccagacgccg acgctgactt cccgtccacc cattcggagt tgcacgacgg
     4141 tggggagacg ctcgagcggg gtgagtgacg cgtcgaagtg ctcgtccgcg tcttcctgcc
     4201 acccgtcgag ccgcacgcgc tgatcgaagt acccgcgcgt atagaggagg ccgacgccga
     4261 ccagcgggac gccgagatcc gaggcggcct tgcagtgatc gccggccagg atgccgaggc
     4321 cacccgagta gatgggcacc gaattatgga acgcgaactc ggcacagaaa tacgcggccg
     4381 ggccctgcag ctccgggtgc tcgtgcctga accaggtacc ctgccctgag gtccgatgcg
     4441 cttcctgctc catcacctgg tcgtagcgct tgaggaaatc ggcgtcggcg gcgagggccg
     4501 cgagacgggc cggatccacg cgcttgagca gctcgatcgg gttgtgcctg gtgagatgcc
     4561 agagcgtctg gtcgatggaa cggaggagct gccgggcgtc ctggctccag ctccaatcga
     4621 ggttccgggt gagcgccgcg agtccggcga tgcggggcgg caggggcagc gacagcgggg
     4681 gtgtagaccc ctccggtgat ccagtcatat aggggaagga acctactggg ctagacgaaa
     4741 aactccacta cacccgtctc aagtgcgtat tccgcaccca ctaccatgag tccgccgttc
     4801 ttgatgagac cctccaggag gcgcgagcca tgccggagct gcccggcgga cgc
//
LOCUS       DASUBP010000092         8695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1324251, whole genome shotgun sequence.
ACCESSION   DASUBP010000092 DASUBP010000000
VERSION     DASUBP010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 8695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8695
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1324251"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1171
                     /locus_tag="VFU75_03505"
     CDS             <1..1171
                     /locus_tag="VFU75_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU5465927.1"
                     /translation="ILTEVGRALGYAHKSNIVHRDIKPDNIMFDDLGQAVVTDFGIAK
                     AASGGKLTGTGMAIGTPHYMSPEQARAQSLDGRSDIYSLGVVAYVCLTGAVPFDGEDS
                     FAIGYKHIMEALPVPELKTDEHRHIFEIVKKMMAKVPEERFQTAEQMVEAVEAGGFVA
                     TGISTAATRAMPSVFGPRVTTGAPGRGDAAEGEAAKPKSTVGGLAIFLAALLVVGGAV
                     WYFGIQQGLISRLLSKAVPIAPIDTTTPAAKESTSVAGTVPAAADTAKHDTAHAPPAT
                     PKPAPPLAVICAEPGPEYNRRKECFDTPPRPTVATFISLPADAPEVPRLAILYIHVSP
                     DGQTLESRLFSPSNVDSITTKAMEMAKTLVWTPALKAGTPVDAWVQQPFVPRRQN"
     gene            1210..1671
                     /locus_tag="VFU75_03510"
     CDS             1210..1671
                     /locus_tag="VFU75_03510"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEU5465928.1"
                     /translation="MLGDVTLEAKSSVWYGCVLRGDMAPIVVGECSNVQDGTIVHVDE
                     GKPARIGARVGIGHRAIIHGCTIEDDCLIGMGSILLNDVVVGTGSVIAAGAVVREGMV
                     IPPGSLVMGVPGRVVRQVDHTLTARIRGTWEHYVAEAVRHRSGRFPLQGNA"
     gene            1859..4363
                     /locus_tag="VFU75_03515"
     CDS             1859..4363
                     /locus_tag="VFU75_03515"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12-dependent ribonucleotide reductase"
                     /protein_id="HEU5465929.1"
                     /translation="MTASNSRKGALDLSANALTVLEKRYLVKDDAGKPTEKAEDLFWR
                     VARTIAAPDAKYGASEKAVAGLAETFFELMATRAWMPNSPTLMNAGRPLGQLSACFVL
                     PVEDALSNGKSGIYDTLRSMALVHQSGGGTGFSFSRLRPKNDVVRSTMGVASGPVSFM
                     KLYDASTDVVKQGGTRRGANMGILRVDHPDIMEFITCKEDLTQVTNFNISVAVTDAFM
                     QAVEAGSSYDLIHPKSKKVVGQLDAREVFRKIVHGAWQTGEPGVYFIDRANYYNPVPH
                     LGSYEATNPCGEQPLLPYDVCNLGSINLGLFIKNGDVDWDRLRTVVHLCTHFLDNVID
                     ANKYPLPEIDDLAKRIRRVGLGVMGWADLLVELGIPYNSDEGVALGKKMMAFIEEESL
                     VASEKLATQRGPFAEWEKSIWGPDATCARGPKGERIRPMRKLRNCNLTTVAPTGTISI
                     ISGCSSGIEPLFAVAFLRNQAGALMPDVNEQFVAIAKREGWYSEDLMRKIAEAGHIHF
                     DEVPAKWQRTFVTAHDVTPEWHIQMQAAFQEFTDSAISKTCNFSHDATEDYVEKIYRD
                     AFKLGCKGVTVYRDGAREGQVLSTGTTAKKVQEHATESGSAAPVEASRAEVAETAGRI
                     AELEAELERTRAQLHDAEAENLQRRAKRSRPDLLRGATRRVETPLGTMYVTITEDDKG
                     QAFEVFASLGKAGAPIMADVEAMGRLISLALRSGIPMKEIHRQLRGISSDRVVGLGPH
                     KVLSVPDAIGIAIEKWMHEKQGIQQDLLSGAAPSPAGSSAVPAREVITGMEGTLGMRE
                     GDGAQDFIGACPDCGSQLAFIEGCAKCHVCGFSECG"
     gene            4425..4802
                     /locus_tag="VFU75_03520"
     CDS             4425..4802
                     /locus_tag="VFU75_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465930.1"
                     /translation="MTAIAPTRLQPKGWARVGPSGAYALRRGAWYPVVRVSTSSIVVL
                     DVNRQNVPVDRRFLELRYHPPERWSVVHCEPATMRRLNREFPLTYGVCPACRHRQGFE
                     MRPAELTCDRCHQRAHVAWDELS"
     gene            complement(4905..5483)
                     /locus_tag="VFU75_03525"
     CDS             complement(4905..5483)
                     /locus_tag="VFU75_03525"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HEU5465931.1"
                     /translation="MRPIGRLVLLQVQRTTLKTGVKPNRTYDPTPLLRVPRAAVSPDG
                     VMGWGDRDAWVVDIHHRAHPNTKNDDGVHGVSVGFTSHYEAMRARFGDLLTLGCAGEN
                     LIAETAAMITADEITRGLVVTGPDGAEKVQLTVLKAAEPCRPFTAWAQGRQVESAVLK
                     ENLQFLQNGMRGFYLQAEGSAVVEVGDLLHAM"
     gene            5534..5896
                     /locus_tag="VFU75_03530"
     CDS             5534..5896
                     /locus_tag="VFU75_03530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465932.1"
                     /translation="MAHLGWARCTDRIADTLRRGAWYPIVEAVGEESVVVAVANRRIR
                     LSLHDVRVRQEEPKRWSIVVRTGVLRPTLGGQGRDLVTTYAVCPKCSERQDFTGRPHD
                     LKCDRCNVTSEVDWTELC"
     gene            complement(5893..7620)
                     /locus_tag="VFU75_03535"
     CDS             complement(5893..7620)
                     /locus_tag="VFU75_03535"
                     /inference="COORDINATES: protein motif:HMM:NF028272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative ABC exporter domain-containing protein"
                     /protein_id="HEU5465933.1"
                     /translation="MISAALYLSRRTWANSLRVRLRRLRQPKYMLAFSAGVFYLWFMF
                     LRRGGPVSSLGLLGPDTRETVAVLFLGVILVLNWALQTSETPFPFTLAETQFLFAAPM
                     TRRQVLLFKIFRSQMALVISAVVTVFLFRRGAWSFVTLLHAVGLWLGYAVLNLNGAGM
                     GLLRLSLAQHGVSGVRRRTGTLIVFALLAGALWWVGRQDIPVVTGAFDTGWRAGFTAL
                     SQVLHSGAGAILSWPLRAVVDPFLATTPLEFLSALPAALLVFGVHVWWVVGSSIAFEE
                     AATDHAQRTAKRIAALRAGRGWTEPRSSRAKPLTSAALNPAGSAAGAIAWKNRLGIKR
                     EFTMRSLVLVGVATVVGVMSLRGPRTSSTELLGAFLLFGAGMVTLLGPLALRFDLRRD
                     LEMLEVLKTYPVRGRTVVLGEVGALLLVLSLAAGALTTAAFLVTITNDSLPALADRLA
                     ILVSALAGIPAVIAVFLLVQNTSVLLFPAWVTVGPQRAVGLEATGQRLLLTIGSLLAL
                     VFALIPAALLFGAVMWLSGDVVGGPWSVALGAVAGAATVGGECWVAVQLLGGVFDRMD
                     PSSAGIASS"
     gene            complement(7630..8352)
                     /locus_tag="VFU75_03540"
     CDS             complement(7630..8352)
                     /locus_tag="VFU75_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5465934.1"
                     /translation="MLQVRGLTRLYDQLVAVEDLSFSVAPGEVLGLVGPNGAGKTTTL
                     RSLCGVIQPTRGTVEIGGVDLGRDPVRAKGQIAFMPDEPHLFDYLTVEEHLRFTARLY
                     KATGAEARIPALLTELELSEKASALPTELSRGMRQKLVIACGLIHDPKALVFDEPLTG
                     LDPAGIRRMKQTIVDRARQGAAVLLSSHLLHLVEEICTRVLILQRGRMVAIGTIPEIL
                     AGRPDLQGKSLEDIFLALTGSA"
BASE COUNT         1539 a         2893 c         2942 g         1321 t
ORIGIN      
        1 catcctcacg gaggtgggcc gggcgctggg gtacgcgcac aagagcaaca tcgtccaccg
       61 cgacatcaag cccgacaaca tcatgttcga cgacctcggc caggccgtgg tcacggactt
      121 cgggatcgcg aaggcggcga gcggcgggaa gctcaccggc acgggcatgg cgatcgggac
      181 gccgcactat atgagtcccg agcaggcccg cgcccaatcg ctcgacggcc gttccgacat
      241 ctactcgctc ggggtcgtgg catacgtctg cctcaccggt gcggtgccgt tcgacgggga
      301 agacagcttc gcgatcggct acaagcacat catggaggcg ctcccggtcc cggagctgaa
      361 gaccgacgag caccggcaca tcttcgagat cgtgaagaag atgatggcca aggtcccgga
      421 ggaacggttc cagaccgcgg agcagatggt ggaagcggtg gaagcgggcg ggttcgtggc
      481 gacggggatc tcgaccgccg cgacccgggc catgccgtcg gtcttcgggc cccgtgtcac
      541 gacgggtgcg ccgggccggg gcgatgccgc cgaaggggag gccgcgaaac cgaagtccac
      601 cgtgggtggc ctcgcgatct tcctggcggc tctcctggtg gtcggtggcg ccgtctggta
      661 tttcggcatt caacagggct tgatcagccg tctgctctcg aaggcggtac cgatcgctcc
      721 catcgatacc acgacgcccg ccgcgaagga gtcgacgtcg gtggccggca ccgttcccgc
      781 cgccgcggat acggcgaagc acgacacggc gcacgctccg ccggcaaccc ccaagccggc
      841 tccacccctg gcggtgatct gcgcggaacc cggccctgag tacaaccgcc ggaaggagtg
      901 tttcgacacg ccgccccggc ccacggtcgc cacgttcatc agcctgcccg ctgacgctcc
      961 cgaggtaccg cggctcgcga tcttgtatat ccacgtctcg cccgacggtc agaccctcga
     1021 gtcccggctg ttctcaccat ccaacgtgga cagcatcacc accaaggcga tggagatggc
     1081 gaagaccctc gtttggaccc ctgccttgaa ggcgggcacg ccggtggatg cctgggtcca
     1141 gcagccgttc gttcccagaa ggcagaactg accctccacc cctcctccta catcgctccc
     1201 ggcgccgtcg tgctcgggga cgtgacgctg gaagccaagt ccagcgtctg gtatgggtgc
     1261 gtcctgcggg gggacatggc gccgatcgtg gttggggagt gctcgaacgt tcaggacggt
     1321 acgattgttc atgtagatga agggaagccc gcacggatcg gggcgcgcgt ggggatcggc
     1381 catcgtgcca tcattcatgg gtgcaccatc gaggatgact gtctcatcgg catggggagc
     1441 atcctgctga acgacgtggt ggtggggacg ggcagcgtca tcgccgccgg cgcggtggtg
     1501 cgggagggca tggtgattcc gcccgggtca ctggtgatgg gggtgccggg ccgcgttgtg
     1561 aggcaggtgg atcacacact cactgcccgg attcgaggca cgtgggaaca ctacgtggcg
     1621 gaggcggtac gtcaccggag cggacggttt ccgctccagg gaaatgcgta agacagattt
     1681 gtgatgttga ttgtcaagac ccgtggtact ttcgaaggtg tcgtcagagc ttgctcgggc
     1741 gtttggaccc acctaccttg ggcctccgcg cttctgattc cagcgagcgc acgccgccat
     1801 caaccttctc agcgcctaag gctctgtgta acgctcttac cgtgagggac gagcccgcat
     1861 gactgcttcc aattcccgca aaggcgcgct cgatctgagc gccaacgccc tcaccgttct
     1921 cgagaagcgg tacctcgtga aggacgatgc cggcaagccc accgaaaagg cggaagacct
     1981 tttctggcgg gtggcccgaa ccatagccgc tccggacgcc aagtacggcg cgtcggagaa
     2041 ggcggtggcc ggactggcgg agaccttctt cgagttgatg gcgacgcggg cctggatgcc
     2101 gaattcgcct accttgatga acgccggacg tcccctcggc cagctgtctg cctgtttcgt
     2161 gcttcccgtc gaggacgccc tgtccaacgg gaagagcggg atctacgaca ccctgcgctc
     2221 catggccctc gtgcatcagt ccggcggcgg cacgggcttc tccttctcgc gcctccgtcc
     2281 caagaacgac gtcgtccgct ccaccatggg tgtcgcctcc gggcccgtct cgttcatgaa
     2341 gctgtatgac gcgtcgacgg acgtcgtgaa gcagggcggg acccgccggg gcgccaacat
     2401 gggcatcctg cgcgtcgacc acccggacat catggagttc atcacctgca aggaagacct
     2461 cacgcaggtg accaatttca acatctcggt cgcggtgacc gacgcgttca tgcaggcggt
     2521 ggaggcgggg tcgagctacg acctgattca cccgaagtcg aagaaggtcg tgggccagct
     2581 ggacgcgcgc gaggtcttcc gcaagatcgt gcacggcgcc tggcagaccg gtgaaccggg
     2641 cgtctacttc atcgaccgcg ccaactacta caatcccgtc ccgcacctcg gcagctacga
     2701 ggcgaccaat ccgtgcgggg agcagccgct gctcccctac gacgtctgca acctgggttc
     2761 catcaacctc ggcctcttca tcaagaacgg ggacgtggat tgggaccgcc tgcggacggt
     2821 ggtccacctc tgcacgcact tcctcgacaa cgtcatcgac gcgaacaagt acccgctgcc
     2881 cgagatcgac gatctcgcca agcggatccg ccgggtgggg ttgggcgtga tgggatgggc
     2941 cgatctcctg gtcgagctcg gcatcccgta caactccgac gaaggggtgg cgctcggcaa
     3001 gaagatgatg gccttcatcg aagaagaatc gctggtggcg tcggagaagc tggccacgca
     3061 gcgcggcccg ttcgcggaat gggagaagtc gatctgggga ccggacgcga cgtgcgcccg
     3121 cgggcccaag ggcgagcgca tccgcccgat gcggaagctc cggaactgca acctgaccac
     3181 cgtggcgccc accggcacca tctcgatcat ctcggggtgc tcgtcgggga tcgagccgct
     3241 gttcgccgtt gcgttcctcc ggaaccaggc cggcgcactg atgcccgacg tcaacgagca
     3301 gttcgtcgcc atcgccaagc gtgagggctg gtattccgaa gacctgatgc ggaagatcgc
     3361 ggaggcgggg cacatccact tcgacgaagt gccggccaag tggcagcgca ccttcgtgac
     3421 cgcgcacgat gtgacgcccg agtggcacat ccagatgcag gcggcgttcc aggagttcac
     3481 cgactcggcg atctccaaga cctgcaactt ctcccacgac gccaccgaag actacgtcga
     3541 gaagatctac cgcgacgcct tcaagctcgg ctgcaagggc gtgacggtgt atcgcgatgg
     3601 ggcgcgcgaa ggtcaggtgc tctcgaccgg cacgacggcg aagaaggtgc aggagcacgc
     3661 caccgagtcc ggatcggctg cgcccgtaga agcctcccgc gctgaagtcg ccgagaccgc
     3721 cggccggatc gcggagctcg aggcggagct cgagcgcacc cgcgcccagc tgcacgacgc
     3781 cgaggcggag aacctccagc ggcgggcgaa gcggtcgcgt ccggatctcc tccgcggcgc
     3841 cacgcgccgg gtcgagacgc ccctcggcac catgtacgtg accatcaccg aagacgacaa
     3901 gggtcaggcg ttcgaagtct tcgcctctct cggcaaggcc ggcgcgccga tcatggcgga
     3961 cgtcgaagca atgggccgcc tcatctcgct cgcgctccgc tccgggatcc cgatgaagga
     4021 gatccaccgc cagctccgcg gcatctcgtc ggaccgggtg gtcggcctcg ggccccacaa
     4081 ggtcctgtcg gtgccggatg ccatcggcat cgcgatcgag aagtggatgc acgagaagca
     4141 ggggatccag caggacctcc tgtccggcgc cgcgccgtcc ccggccgggt cgtcggccgt
     4201 tccggcgcgc gaagtgatca ccgggatgga gggcaccctc ggcatgcggg aaggggatgg
     4261 cgcccaggac ttcatcggtg cctgcccgga ctgcgggtca cagctcgcgt tcatcgaggg
     4321 ctgcgccaaa tgccatgtct gcggcttcag cgagtgcggc tgatccgccg gaaattgtga
     4381 ggcttgtcac aacgccgcgt gacggagccg attaaatctc tggcatgaca gcgatagccc
     4441 ccacccggct ccaaccgaag ggttgggccc gcgtcggtcc ctccggcgcg tacgcacttc
     4501 gacggggtgc ctggtacccc gtcgtccgag tgtccacctc gagcatcgtg gtgctggacg
     4561 tgaaccggca gaacgtgccg gtcgatcgtc gcttcctcga gctccgatat catccacccg
     4621 agcgttggag cgtggtgcat tgcgagcccg cgaccatgcg tcggctcaac cgcgaattcc
     4681 cgctcaccta cggcgtctgt cccgcctgcc ggcaccggca aggcttcgag atgcggccgg
     4741 cggagctgac gtgcgaccgg tgtcatcagc gagcccacgt ggcttgggat gagctgagct
     4801 aggggccggg ggtcaggggt caggggtcag aaaaacagga aggggggctt cgatgagctc
     4861 ccctttcgtg tttaccctga cccctgatcc ctgacccccg atccctacat cgcgtgcagc
     4921 agatctccca cttcaaccac cgcgctcccc tccgcctgca gatagaaccc ccgcatcccg
     4981 ttctggagga actgcaggtt ctccttcagc accgccgact cgacctgcct gccctgggcc
     5041 cacgccgtga acgggcggca cggctccgcc gccttcaaga ccgtgagctg gaccttctcc
     5101 gcgccgtccg ggcccgtgac caccagaccg cgggtgatct cgtcggccgt aatcatcgca
     5161 gcggtctcgg cgatcaggtt ctcgccggcg cagccgagcg tcagcaagtc tccgaaccgg
     5221 gcccgcatcg cttcgtagtg cgacgtgaac cccaccgaga cgccgtgcac gccgtcgtcg
     5281 ttcttggtgt tggggtgggc ccggtggtga atgtccacca cccaggcgtc ccggtctccc
     5341 cagcccatca cgccgtcggg gctaaccgcc gcgcgcggga cgcgcaggag gggggtcggg
     5401 tcgtaggtcc ggttcggttt gacgccggtc ttgagggtcg tgcgctgtac ctggagtagt
     5461 accagtcggc cgatcggtcg cataacggaa tttagcggaa aggagtatct tcttgcgact
     5521 gagagggcac tcaatggcac accttggctg ggcccggtgc accgaccgga ttgccgatac
     5581 gctccggcgc ggcgcgtggt acccgatcgt ggaagcggtc ggagaggagt ccgtggtggt
     5641 agcggtggcg aaccgccgca tccggctctc gctccacgac gtgcgcgtgc gtcaggaaga
     5701 acccaagcgc tggagcatcg tcgtgcgcac gggcgtcctg cggcccacac tgggcgggca
     5761 ggggcgggat ctggtcacca cctacgccgt gtgcccgaag tgttccgagc gccaggattt
     5821 caccggacgg ccccacgatc tcaagtgcga tcgttgcaac gtgacgtccg aggtggactg
     5881 gacggagctc tgctaggaag aagcgatccc cgctgacgac ggatccatcc ggtcgaatac
     5941 gccaccgagc agctgcaccg ccacccagca ctctccaccc accgtcgccg cacccgctac
     6001 tgcaccgagc gccaccgacc agggcccgcc caccacatca ccgctgagcc acatcaccgc
     6061 gccgaacagc agggcggcgg ggatcagcgc gaacaccagc gccagcagcg aaccgatggt
     6121 caggagcagc cgctgaccgg tcgcctcgag acccaccgcc cgttgcggac cgaccgtcac
     6181 ccaggcgggg aacagcagca ccgaggtgtt ctgtaccagc agaaagacgg cgatcaccgc
     6241 cgggatcccg gcgagcgcgc tgaccaggat ggccaggcgg tcggcgaggg ccggcaggga
     6301 gtcgttggtg atggtcacca ggaacgcggc cgtcgtgagc gcgcccgcgg cgagagagag
     6361 gacgagcagc agagcgccga cctcgcccag gaccaccgtg cgtccccgca ccgggtacgt
     6421 cttcaggacc tcgagcatct ccagatcacg ccggaggtcg aaacgcagcg cgagcggacc
     6481 caggagcgtg accatcccgg cgccgaagag cagaaacgcg ccgaggagct cggtggacga
     6541 ggttctgggg ccccgcaggg acatgactcc caccacggtc gcgacgccga ccagcacgag
     6601 ggaccgcatg gtgaactcgc gcttgatgcc gagccggttc ttccacgcga tggctccggc
     6661 ggcggagccg gcgggattga gggccgcgga cgtgaggggc ttggcacggc tgctccgggg
     6721 ctcggtccac ccgcgtcccg cgcgaagcgc ggcgatgcgc ttcgcggtcc gctgggcgtg
     6781 gtcggtcgcc gcctcctcga acgcgatcga cgacccgact acccaccaca cgtgcacgcc
     6841 gaagacgagg agcgccgcgg gcaaggccga caggaactcg aggggtgtgg tcgccaggaa
     6901 gggatcgacc acggcgcgca ggggccagga caggatcgcg ccggcgccag agtgcaggac
     6961 ctgactcagc gcggtaaagc cggcccgcca ccccgtatcg aaggccccgg tgaccaccgg
     7021 aatgtcctga cgccccaccc accagagagc cccggccaag agtgcgaata cgatcagggt
     7081 gccggtgcgt cgccgtacgc cggaaacccc gtgttgggcg agggagagcc ggagcaggcc
     7141 catccccgcg ccattgaggt tcagcacggc gtaccccagc cagagcccca cggcgtgcag
     7201 cagggtcaca aagctccacg cgccgcggcg gaacaggaac acggtcacta cggcggagat
     7261 cacgagcgcc atctgcgagc ggaaaatctt gaagagcagc acctgcctgc gggtcatggg
     7321 tgccgcgaac aggaactgcg tctcggccag cgtgaacggg aagggggtct cgctggtctg
     7381 gagggcccag ttgaggacca ggatcacgcc gaggaacagc accgccaccg tttcccgggt
     7441 gtcgggcccg aggaggccca aagagctcac gggcccgccg cggcggagaa acatgaacca
     7501 caggtagaac acccccgccg agaacgcgag catgtacttc ggctgcctga ggcgccgcag
     7561 gcgcactcgg agggaattgg cccacgtccg ccggctgagg tatagcgctg cggaaatcac
     7621 gactctgacc taggccgacc cggtgagcgc gaggaagatg tcctcgaggc tcttcccctg
     7681 gaggtcgggg cgcccggcca ggatctcggg gatggtcccg atggccacca tccggccccg
     7741 ctggaggatc aggacccggg tgcagatctc ctccaccagg tggaggaggt gggaggacag
     7801 aaggacggcg gccccctgcc gggcccggtc cacgatggtc tgtttcatcc gcctgatccc
     7861 ggcggggtcg agcccggtca ggggctcgtc gaacaccagg gccttggggt cgtgaatcag
     7921 accgcaggcg atcaccagtt tttggcgcat cccgcgggac aactcggtgg gaagggcgga
     7981 ggccttttcg gacagctcca gctcggtcag cagggcaggg atccgggcct cggctccagt
     8041 ggccttgtac agccgggcgg tgaaccggag gtgctcttcg acggtgaggt agtcgaagag
     8101 gtggggctcg tcgggcatga acgcgatctg acccttggcc cggaccgggt cccgccccag
     8161 atccaccccc ccgatttcga ccgtgccccg ggtgggctga atgaccccgc agagcgagcg
     8221 cagggtggtg gtcttgccgg cgccattcgg cccgaccagt cccagcactt cgccgggcgc
     8281 gaccgagaac gacaaatcct cgaccgcgac gagctggtcg tacaggcggg taaggccgcg
     8341 gacctggagc atggccccga caatctaggc ggggcgggga cttgcgggag gcgacatgtc
     8401 gtttatatat taacagtcaa catattaaca ttcgctgacc cgcactgctt tgtgagtgtc
     8461 gctaaacaag agacgccaag gcgctaagtc gggaaggcgc tacgtggggg ggacgcatga
     8521 gtgaccgatc ggagctggcc ctccagctca ccgacgccgt gaccgcggcc caatccgccg
     8581 tcgagcggtc gggggaccgt gccttccgca cctacgggct cacgcatcgg gcccacacgg
     8641 ccctctacgc cgtcgatcag ggtggacccg acgggatcag gccccgggag gtcgc
//
LOCUS       DASUBP010000093         4837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_411031, whole genome shotgun sequence.
ACCESSION   DASUBP010000093 DASUBP010000000
VERSION     DASUBP010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4837
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411031"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1030
                     /gene="aroC"
                     /locus_tag="VFU75_03545"
     CDS             <1..1030
                     /gene="aroC"
                     /locus_tag="VFU75_03545"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEU5465935.1"
                     /translation="MKIESDHIEWLAGVRAGETLGSPIAMLIWNRDWEHWKDVMAPEA
                     DAPGEERRRQVTRPRPGHADLVGVMKFDRKDARDILERASARETVARVACGAVCKALL
                     GQFGVEIGSHVAELGGVVANVHGALPSPLNPAADASVVRCLDADAEREMIARIDAAKA
                     AGDTLGGIVEVVARGVPVGLGSHVSWDTKLDGQLAQALMSIPAVKGVELGMGFEAARK
                     KGSEVHDEIMPGLSRATNRAGGTEGGMTTGEPLTVRVAMKPISTLMTPLKTVDLKTGA
                     TAQAQSERSDVTAVPAMGVIAEAMVALVLAHAMREKFGGDALSEMRRNWEGYAARLAA
                     RASGGMHS"
     gene            1027..1545
                     /locus_tag="VFU75_03550"
     CDS             1027..1545
                     /locus_tag="VFU75_03550"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEU5465936.1"
                     /translation="MKRHIVLVGLPGSGKTTVGRLLAEQLQAGFVDVDSILVRKEGKP
                     VTMIFAEKGEAAFRAMEQQEMENALGQVPAVIAPGGGWAAQPGAISSAKPQAFVVYLK
                     TSPQTASDRAAPEGTRPLLMGEDPVERMKALLKEREAAYLQADAKVETDKKTAAQVAQ
                     DLVSLARSGAGW"
     gene            1618..4050
                     /gene="topA"
                     /locus_tag="VFU75_03555"
     CDS             1618..4050
                     /gene="topA"
                     /locus_tag="VFU75_03555"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEU5465937.1"
                     /translation="MKKKATAEKKPIPAPAPAPKKRGGKKRASPVVEVMEDSAEGAET
                     EAEETAATAGSTNGKGSLVIVESPTKAKTIGKYLGRGYTVKATIGHLRDLPRRKLGVD
                     VDNGFEPEYVTIKEKAKTLAEIKKAARAASQVLIATDPDREGEAIAWHVAGQLGKRDK
                     VRRVLFHEITKDAVKLALANPLDIDQKKVDAQQARRILDRLVGYKASPLLWKSIKTGL
                     SAGRVQTVALRLICEREDEIRKFVPQEYWTIEADLEKDGQSFQARLHKLDGAKPDIKD
                     QRAAQGIVDEATKIPFVVTDVTKKERRRNPPAPFITSTLQQEAAKQLGFSAKRTMRAA
                     QGLYEGVDVGDGPVGLITYMRTDSVRVADSAIEQAREFINANYDKRYLPETPNVHAGK
                     RGGNARVQDAHEAIRPTEVGRRPEDMKKYLESDLFKLYQLVWRRFVASQMTPEVYELT
                     TVDFDMGRFLFRSTGSVVLFDGYRVLFREGHEAEEAKNADDLAPIPPLAPGDRPTLKQ
                     ITPTQHFTEPPPRYSQASLVKMLEEQGIGRPSTYAAIISTLQARWYATMKERRFSPTP
                     LGEKVWKVMQRCFPDTFQVGFTAQMETELDKVEEGDMPWQEVLGGFWTPFAGQLAAID
                     VPRIINDVHDLHNLHLERCPTCGSPLEVRSGRFGPYIACAKYPTECKFSKPIGRDKVP
                     DKPTDEICKECGAPMVIKTGRFGEFLACTRYPKCTHTRPVPLGIKCPKCEVGDLAERR
                     TKKGRNFYGCLRYPDCDFSVWNKPVAVNCPSCGYLGMEEKRTKTMGVTRKCLKCGHEM
                     TVDEAATAEVAG"
     gene            3996..>4837
                     /gene="trmFO"
                     /locus_tag="VFU75_03560"
     CDS             3996..>4837
                     /gene="trmFO"
                     /locus_tag="VFU75_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEU5465938.1"
                     /translation="MRTRNDGGRGSHRGGRGLTAAVVGGGLAGCEAAWALAERGVSVT
                     LHEMRPVRGTPAHQTDSLAELVCSNSFKSVETVNAHGLLKAEMRLLGSINLQAADVAR
                     VPGGAALAVDRVAFAQAIQERIGGHPNIRLVRGEMTSLESPAIVATGPLTSEALSAAI
                     VSRLGGQALAFWDAIAPIVSTESLDQSVLFRASRYGKGDGDDYLNAPFTEAEYDAFVT
                     ALKVGDQYAPNHEFDKIQYFEGCLPVEEMAARGRDVLRFGPMKPVGLPDPKTGRQAFA
                     VVQLR"
BASE COUNT          902 a         1536 c         1704 g          695 t
ORIGIN      
        1 catgaaaatc gagtccgacc acatcgagtg gctcgcgggc gtccgcgccg gcgaaaccct
       61 cggctccccc atcgcgatgc tgatctggaa ccgcgactgg gagcattgga aggacgtcat
      121 ggctcccgag gccgacgccc cgggagaaga acggcggcgg caggtgacgc ggccgaggcc
      181 gggccacgcg gacctggtcg gcgtcatgaa gttcgaccgg aaggacgcgc gggacattct
      241 cgagcgcgcg agtgcccggg agacggtcgc gcgggtcgcg tgcggcgcgg tgtgcaaggc
      301 gctcctggga cagttcgggg tggagatcgg cagtcacgtg gcggagctgg gaggagtggt
      361 cgcgaacgtc cacggcgcgc tcccttcgcc cctcaatccc gcggctgatg cctccgtggt
      421 ccgttgcctg gacgccgatg ccgagcgcga gatgatcgcg cggatcgatg cggcgaaggc
      481 cgccggagat acgctgggcg ggatcgtcga agtcgtggcc cgtggcgtgc cggtggggtt
      541 gggctcgcac gtgtcgtggg atacgaagct cgacggccag ctcgcgcagg ccctgatgtc
      601 cattcctgcc gtgaagggcg tggagctggg gatggggttc gaggcggcgc ggaagaaggg
      661 gtcggaagtg cacgacgaga tcatgccggg cctctcgcgc gccacgaatc gcgcgggcgg
      721 caccgaaggc ggtatgacca ccggggaacc gctgacggtt cgtgtggcca tgaagccgat
      781 ctcgaccttg atgacgccgc tcaagacggt cgatctgaaa acgggtgcca ccgctcaggc
      841 gcagtcggaa cggtccgatg tgaccgccgt gccggcgatg ggcgtgatcg cggaagcgat
      901 ggtggccctc gtgctggcgc atgcgatgcg cgagaagttc ggcggggatg cgctgtcgga
      961 aatgcgccgc aactgggaag ggtacgccgc gcggctggcc gcgcgcgcgt ccggagggat
     1021 gcattcgtga agcgacacat cgtgctggta ggcttgcccg gctcggggaa gaccaccgtg
     1081 gggcggctcc tggcggagca gctgcaggcc ggcttcgtgg acgtcgactc cattctggtg
     1141 cgcaaggaag gcaagcccgt caccatgatt ttcgcggaga agggcgaagc cgcgtttcgc
     1201 gccatggaac aacaggagat ggagaacgcg ttagggcagg tgccggcggt catcgccccc
     1261 gggggcggct gggcggcgca accgggcgcc atttcctcag cgaagcctca ggcgtttgtg
     1321 gtgtatctga agacgagccc tcagacggcg tcggaccgcg cggcccccga gggaacccgg
     1381 ccgttgttaa tgggggagga cccggtggag cggatgaagg cgctcctgaa ggagcgcgag
     1441 gccgcgtatc tccaagcgga cgcgaaggtt gagacggaca agaagacggc ggcgcaggtg
     1501 gcgcaggatc tggtctccct tgcgcgaagc ggtgcaggtt ggtgaagatg cctccccgcg
     1561 gccgtcccaa ggccgttgat gatgaaaggt tgatcagtgg ctgctaagaa gaaacccgtg
     1621 aaaaagaaag caacggcgga aaagaagccc attcccgctc ccgcccccgc gcccaagaag
     1681 cgcggcggga agaagcgggc ctcgcccgtc gtcgaagtca tggaggattc ggccgaagga
     1741 gcggaaaccg aggccgaaga gaccgccgcg acggccgggt ccacgaacgg gaagggctcg
     1801 ctcgtcatcg tcgagtcccc caccaaggcg aagaccatcg ggaagtacct gggccggggc
     1861 tacacggtga aggcgaccat cggccacctc cgggatctcc cccgccgcaa gctcggcgtg
     1921 gacgtcgata acgggttcga gcccgaatac gtgaccatca aggagaaggc caagaccctc
     1981 gccgagatca agaaggcggc aagggctgcg tcccaggtcc tgatcgccac cgatccggac
     2041 cgcgaaggtg aagccatcgc gtggcacgtg gcgggacagc tcggcaagcg cgacaaggtc
     2101 cggcgcgtgc tgttccacga aatcacgaag gacgcggtca agctcgccct cgcgaacccc
     2161 ctcgacatcg atcaaaagaa ggtggacgcc cagcaggccc gccggatcct cgaccggctg
     2221 gtgggctaca aggcctcccc cctcctctgg aagagcatca agacgggact ctccgccggc
     2281 cgggtgcaga cggtggccct ccggttgatc tgcgagcggg aagacgaaat ccggaagttc
     2341 gtgccgcagg agtactggac catcgaggcc gacctcgaga aggacggcca gtcgttccag
     2401 gcgcgcctcc acaagctgga cggcgccaaa cccgacatca aagaccagcg ggcggcgcag
     2461 gggatcgtcg acgaagcgac gaagatcccc ttcgtcgtca ccgacgtcac caagaaggaa
     2521 cgccgccgga atccgcccgc ccccttcatc accagcacct tgcagcagga agccgccaag
     2581 cagctcggct tctcggcgaa gcgcaccatg cgggccgccc aggggctcta cgagggcgtg
     2641 gacgtggggg atgggccagt cggcctcatc acctacatgc gaaccgactc cgtccgtgtg
     2701 gcggactcgg ccatcgagca ggcgcgcgag ttcatcaacg cgaactacga caagcggtac
     2761 ctccccgaaa cgccgaacgt tcatgcgggc aagcgcggcg ggaacgcccg ggtgcaggac
     2821 gcccacgaag cgatcaggcc gacggaagtc ggccgccggc ccgaggacat gaagaagtac
     2881 ctcgagtcgg acctgttcaa actctatcag ctcgtctggc ggcgcttcgt cgcgtcccag
     2941 atgacgcccg aggtctacga actcaccacc gtggacttcg acatgggacg cttcctgttc
     3001 cggtccaccg ggtcggtggt cctcttcgac ggctaccgcg tcctcttccg cgagggtcat
     3061 gaagccgagg aagccaagaa tgcggacgac ctcgccccca ttccgcccct cgcccccggc
     3121 gaccgtccga cgctcaagca gatcacgccg acgcagcatt tcacggagcc gcctccacgg
     3181 tacagccagg cgtcgctggt gaagatgctc gaggagcagg ggatcggccg tccgtccacc
     3241 tacgcggcga tcatctcgac gctccaggcg cgctggtacg cgaccatgaa agagcgccgc
     3301 ttctcgccca cgccactcgg cgaaaaggtg tggaaggtga tgcagcgctg cttccccgac
     3361 acgttccagg tcgggttcac ggcgcagatg gagaccgagc tggacaaggt ggaagagggc
     3421 gacatgccct ggcaggaggt gctgggcggg ttctggaccc cgttcgcggg gcagctggcc
     3481 gcgatcgacg tgccgcggat catcaacgac gtccacgatc tccacaactt gcacctcgag
     3541 cgctgtccga cgtgcggttc gccgctcgag gttcgttcgg gacggttcgg cccctacatc
     3601 gcgtgcgcca agtacccgac cgagtgcaag ttctcgaagc ccatcggccg ggacaaagtc
     3661 ccggacaagc cgacggacga gatctgcaag gagtgcgggg cgccgatggt catcaagacc
     3721 gggcgcttcg gcgaattcct ggcgtgcacg cggtacccga agtgcaccca cacccggccc
     3781 gtccccctcg gcatcaagtg tccgaagtgc gaggtcggtg acctggccga gcggcggacc
     3841 aagaagggac gcaacttcta cggatgcctc cggtatcccg actgcgactt ctcggtctgg
     3901 aacaagccgg tggcggtgaa ctgcccgtca tgcggctatc tgggaatgga agagaagcgg
     3961 accaagacga tgggggtcac ccgcaagtgc ctgaagtgcg gacacgaaat gacggtggac
     4021 gaggcagcca ccgcggaggt cgcgggctga cggccgcagt agtcggcggg ggcttggcgg
     4081 ggtgtgaagc cgcctgggcc ctggccgaac gtggggtatc cgtcaccctc cacgagatgc
     4141 gcccggtgcg cggtactccc gcccaccaga ctgattccct ggccgagctg gtctgcagca
     4201 actccttcaa atcggtcgag accgtcaacg cccatgggct cctcaaggcc gagatgcgcc
     4261 tgttggggag catcaatctc caggccgccg atgtggcgcg ggtgccaggg ggagcggcac
     4321 tggcggtcga ccgcgtcgcg ttcgcccagg cgattcagga gcgaatcggg ggccacccga
     4381 acatcaggct cgtccgcggc gaaatgacga gcctcgagtc tccggcgatc gtcgccaccg
     4441 gtcccttgac ctccgaggct ctgagcgccg cgatcgtgag ccggctgggt ggacaggccc
     4501 tcgcgttctg ggacgccatc gcgccgatcg tctcgacgga gtccctcgac cagagcgtgc
     4561 tcttccgcgc gtcccggtac gggaagggtg acggcgacga ctatctcaac gcgccgttca
     4621 ccgaggccga atacgatgca ttcgtgacgg cgctgaaggt gggggaccag tacgcgccga
     4681 atcacgagtt cgacaagatc cagtacttcg aagggtgcct gccggtggag gagatggcgg
     4741 cgcggggacg ggacgtgctc cgcttcgggc ccatgaaacc ggtgggcctg cccgatccca
     4801 agacgggccg gcaggccttc gcggtcgtcc agctccg
//
LOCUS       DASUBP010000094         8472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_520230, whole genome shotgun sequence.
ACCESSION   DASUBP010000094 DASUBP010000000
VERSION     DASUBP010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 8472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8472
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_520230"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..865
                     /locus_tag="VFU75_03565"
     CDS             23..865
                     /locus_tag="VFU75_03565"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEU5465939.1"
                     /translation="MSGPTLVILAAGMASRYGGVPKQLEPIGPGGATLMDYSLHDARR
                     AGFTSAVLVIRSELEPVFRERLIPSWMPTFPISVAYQEIVAPRTRPWGTGQAVLAARP
                     AVHGPFAVINADDFYGRSAIMSLRKFLGEPATHPPTAGLVGYPLAATLSDAGGVTRAV
                     LHADGSGSLDSITEVRGIRDPAAFSPGTVVSMNAWAFPAAVFDFLDARFADFQMTHAQ
                     DNEEFLLPTAIQDLIRRKSLRVKVLPGGERWVGVTYPADRPRAESFLRAETAGGTYPV
                     EPWR"
     gene            856..1950
                     /locus_tag="VFU75_03570"
     CDS             856..1950
                     /locus_tag="VFU75_03570"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HEU5465940.1"
                     /translation="MALAADPALDAAHAFATPGTLAALEPFPGGHIHAAHLVTYRHGG
                     GTTRFLLQQLNTLVFPHPEQVMGNIERVTAHVGKALAKARVRDLDRRNLTLVHTRQGA
                     TWHRDSDGRVWRLYHFIEGAVARSSPRNADDAAAAAEAFGGFQRLLADFPDPPLHQTI
                     PDFHFTPGRMKALEEAVDTDALGRCDSARDEIETVFATHGLAHALLDAHLPERVTHND
                     AKISNLLFDAESGSGLCVVDLDTVMPGFAVYDFGDLVRSMATRAAEDERDLSQVKLEP
                     GLVKAIAWGYLKEAGAFLSSIERTLLITAARVIVLEQAARFLADHLEGDLYYRISRPN
                     HNLDRARTQIRLLEELNVNAAALEREVAKL"
     gene            complement(2035..2874)
                     /locus_tag="VFU75_03575"
     CDS             complement(2035..2874)
                     /locus_tag="VFU75_03575"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU5465941.1"
                     /translation="MSPLLVGALLSSAVIHASWNLLLKTTHHRRPVVWWALIVTNLCC
                     AVILIARPHSITPVWPLLLVSAVLEASYYLILTAAYKVGDFSLVYPVARGTAPGLLAV
                     WAILVLSERPSAFGILGISLIAVGIGVVGWSGRIGKPSKALALSLLLAVVISVYSIID
                     GVAVRRVDPVDYTAAMFFVVTLLLTPVLMGQEGWEGIRGVLRLEPLRIVTIGVMQLAG
                     YLIVLWVYSRAPVSYGGAIRESSILVGALLGWRFLGEPFGARRVVGALVIVTGVILIA
                     LGG"
     gene            complement(3014..4801)
                     /locus_tag="VFU75_03580"
     CDS             complement(3014..4801)
                     /locus_tag="VFU75_03580"
                     /inference="COORDINATES: protein motif:HMM:NF012686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HEU5465942.1"
                     /translation="MELSRLDVVIAIVSIVICFAPALFFARRSGKGTAEFFASGQAAP
                     WWLVGTSMVATTFSTDTPNLVTDFVRSHGVSYNWAWWSFLLTGMATVFFYAKLWRRSG
                     VLTDLQFYELRYSGKPAAVVRGFRAIYLGLFFNLTIMTTVTLAAVKIANVMLGWERLT
                     TIIVGGLTCILFASVSGLWGVLVTDMIQFALAMFGVIAAAVVALDQPQVGGLANLLHR
                     TDPHVLSLLPDFSNWDVAVAVFIIPVTIQWWSVWYPGAEPGGGSYIAQRILASKNEKH
                     ALGATLWFNIAHYALRPWPWIIVALASMLVFPTLGDIQRAIPHVDPRLIGNDLAYPAM
                     LTLLPAGMKGLLIASLFAAYRSTMETHLNWGSSYLVIDFYQRFLARGKSEKHYLWISR
                     GLTATLMVLCGFFTLFISTASEGFQLLLSIGAGTGLIYLLRWFWWRINAWSEIAAMMG
                     SFACSLTLFVGKHAFGWNISDARTLIIVVAVTTVLWITVTMLTKPVDDATLDNFYKLT
                     RPAGAGWRAVRERTKLPPSPDSLPQALLGWTAGCAFVYSALFGAGNALYGRSGQAGVC
                     FVMAIISGIVLYRVISTMFRGEAAPAGRG"
     gene            complement(4826..5443)
                     /gene="msrA"
                     /locus_tag="VFU75_03585"
     CDS             complement(4826..5443)
                     /gene="msrA"
                     /locus_tag="VFU75_03585"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEU5465943.1"
                     /translation="MTAVVPLLAALLSTQPSSAPKAAPLDTAVLAGGCFWGVEGIFEH
                     VKGVVKVVSGYAGGTLANPTSAQVYNGGTGHAESVMIIYDPARVSFDQLLLVFFNVAH
                     DPTQLNRQGPDVGSDYRSIAFYRDSTQKQSVEDAIASLKRRHVYSDPIVTEVLAFRAF
                     YPAEAEHQGFMQRHLSDAYIVTNDLPKLEVLKTRYATLYRAEWHD"
     gene            complement(5531..6121)
                     /gene="msrA"
                     /locus_tag="VFU75_03590"
     CDS             complement(5531..6121)
                     /gene="msrA"
                     /locus_tag="VFU75_03590"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEU5465944.1"
                     /translation="MITPLLIALTTALAPARDTAVFAGGCFWGIEGVFEHTRGVISAT
                     AGYAGGHTASPSYEQVSSGETGHAEAVRVVYDPAQVSYGQLLAVFFTVAHDPTELDRQ
                     GPDVGTNYRSIAFYRDSTQHAAINTFISELQKRRLYSKPVVTEVIPLATFYEAEGYHQ
                     HYMEHHPDQPYIAYNDAPKVAHLKEAFPALYRDSWK"
     gene            complement(6118..7038)
                     /locus_tag="VFU75_03595"
     CDS             complement(6118..7038)
                     /locus_tag="VFU75_03595"
                     /inference="COORDINATES: protein motif:HMM:NF021982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHB depolymerase family esterase"
                     /protein_id="HEU5465945.1"
                     /translation="MTSRALVGVAFCLAAGLGFHQGGDRTEHIRVGGRDRTYLLHVPP
                     NASGARPLVVLLHGRLGDGAGEARLSYFDPIADSVGAIAAYPDGYRRSWADGRLGTPA
                     DKAGIDDVAFFRALVADVAAHAPVDSSRIYVAGMSNGGFMTERLICDASDLIAAAGIV
                     AATLGDSLAGNCHAARSVPVMTMNGTEDPLVPYQGGTLGGGRGTVLGAIPSLDEWVHR
                     NGCAERFKAIVLPDTARDGTTINERVYPCPSGADVVGITVVHGGHTWPSGPQYLPKRM
                     IGISSRNLDGSRTLWTFFAAHRRTNMKETR"
     gene            complement(7035..8450)
                     /gene="cysS"
                     /locus_tag="VFU75_03600"
     CDS             complement(7035..8450)
                     /gene="cysS"
                     /locus_tag="VFU75_03600"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEU5465946.1"
                     /translation="MALFLHNTLTRAIEPFQPLHPPRVTLYTCGPTVWNYAHIGNFRT
                     FLFEDLLRRHLEASGYDVFHIMNLTDVDDRTIKAAAAAGKTLTDHTEQYAQAFFTDRD
                     FLRIRPAHVYPRATQSVPAMVALIEKLLAKGVAYKGDDGSVYFAISKFPTYGRLSRLD
                     TREIKVGARVASDEYAKDDPRDFALWKKSDDEDERVGAAWDASFGRGRPGWHLECSAM
                     SLDEIGKRFKVETLDLHAGGIDLIFPHHEDEIAQSEAATGQTFARTWLHGEFLNLRGT
                     KMSKRYGNILTARDLKEQGVDAASVRLLFFQTHYRQSIDFNDDALAAAAEGVRRLGEF
                     QERLATARPDPKGPLADLAAGMEQNVRAALDDDLNAPQALAHLFEFVRAANREIDAGA
                     GGAPTAQASLRKVMDVLDVMPTKKTVAADLSQWVEERVSARTQARQSRDFKEADRIRA
                     ELKEKGVEIEDTPGGTKWKIG"
BASE COUNT         1514 a         2847 c         2790 g         1321 t
ORIGIN      
        1 tcggcttgta tctggagcat gcgtgagcgg acccaccctc gtgatccttg ccgccggcat
       61 ggcgagccgg tacggcggcg tgcccaagca gctggagccg atcggccccg gcggcgcgac
      121 cctcatggac tactccctcc acgacgcccg gcgcgccgga ttcacctcgg cggtgctggt
      181 gatccggagc gagctggagc cggtgtttcg ggagcggctc atcccgtcgt ggatgccgac
      241 attccccatt tccgtcgctt accaggagat cgtggcccca cgcaccaggc cctggggcac
      301 gggtcaagca gtcctggccg cccgtcccgc ggtccacggt cccttcgccg tgatcaacgc
      361 cgacgacttc tacggccgct cggccatcat gtcgctccgc aagtttctcg gtgaaccggc
      421 gacccatccc ccgaccgcgg gactggtcgg gtacccgctc gcggcgaccc tgtcggatgc
      481 gggaggcgtg acgcgggccg tgctgcatgc ggacggctcc ggctcgctcg actccatcac
      541 cgaagtccgg ggcatccgcg atcccgcggc cttctcgccc ggaaccgtcg tgtcgatgaa
      601 cgcatgggcc tttcccgcgg cggtgttcga cttccttgac gcccgcttcg ccgacttcca
      661 gatgacgcac gcacaggaca atgaagagtt cctgcttccc acggccatcc aggacctgat
      721 ccggcggaag agtctccggg tgaaagtcct gcccggcggg gaacgctggg tgggcgtgac
      781 gtacccggcc gatcggccgc gcgccgagtc gttcctgcgg gccgagaccg ccggcggcac
      841 ctacccggtg gagccgtggc gctagcggcg gatcccgcgc tcgacgcggc ccacgccttc
      901 gcgacgccgg ggacgctcgc cgccctcgag ccgttcccgg gcgggcacat ccatgccgcg
      961 catctcgtca cctaccgcca tggcggcggg acgacgcgct tcctgctcca gcagctcaat
     1021 accctggtct tcccccatcc cgaacaggtg atgggcaaca tagagcgcgt caccgcgcac
     1081 gttggcaagg cgctcgccaa ggcccgggtg cgcgatctcg accggcgcaa cctcaccctg
     1141 gtccacacgc gtcagggggc gacctggcat cgggattccg atggccgggt gtggcgcctc
     1201 tatcatttca tcgagggtgc ggtggcgcgg tcctcccccc gcaatgccga cgacgcggcc
     1261 gccgcggcgg aggcgttcgg gggattccag cgcctgctcg ccgatttccc ggatcctcct
     1321 ctgcaccaga ccatccccga cttccatttc acgccggggc ggatgaaggc gctcgaagag
     1381 gcggtcgata cggacgcttt ggggcgctgt gattcggcgc gcgacgagat cgaaactgtg
     1441 ttcgcgacgc acgggttggc ccacgcactc ctcgacgccc acttgccgga acgcgtgacg
     1501 cacaacgacg cgaagatctc gaacctgctc ttcgatgcgg aaagtggatc gggtctgtgc
     1561 gtggtcgatc tcgataccgt gatgcccgga ttcgcggtct acgacttcgg cgacctggtc
     1621 cgctccatgg cgacgcgggc ggccgaggac gagcgggacc tctcccaggt gaagctggag
     1681 ccgggtctcg tgaaggccat tgcgtggggg tacctgaagg aagccggcgc gtttctgtca
     1741 tccatcgagc gcacgctgct gatcaccgcc gcccgggtga tcgtgctcga gcaggccgcg
     1801 cgctttctcg ccgatcatct cgagggggat ctgtactacc ggatctcgcg gcccaatcac
     1861 aacctcgacc gcgcgcgcac gcagattcgc ttgctcgagg agctgaacgt gaatgccgca
     1921 gcgttggagc gggaggtcgc gaaactctag gcacccaggc tcaagcctgg gccggaccca
     1981 gcggggcgct taagcgcgga gcatcgccgg gcccaccctt gagggtggga acgcctagcc
     2041 gcccagtgcg atcagaatca cacccgtgac gatcaccaga gcacccacta cccgtctggc
     2101 accgaagggc tcaccgagaa aacgccaccc gagaagcgct cccacgagga tgctcgactc
     2161 ccggatggcg cctccataag acacgggggc tcgggagtag acccacagaa cgatgaggta
     2221 ccccgccagc tgcatgacgc cgatcgtcac gatgcggagt ggctcgagcc tgaggacgcc
     2281 gcggatccct tcccatccct cctgtcccat gagcacaggc gtcaggagga gcgtcaccac
     2341 gaagaacatc gccgccgtgt agtccacggg atccacgcgc cgcacggcca cgccgtcgat
     2401 gatcgagtac accgaaatca cgaccgccag gagcagtgag agcgcaagcg ccttggaggg
     2461 cttcccgatc cgccccgacc atccgaccac gccgatgccc accgcgatca gggaaatgcc
     2521 gaggatgccg aaggccgagg gccgctccga gagcacgagg atggcccaga cggcgaggag
     2581 tccgggcgcc gtcccgcggg cgacgggata caccagtgag aagtcgccga ccttgtaggc
     2641 agcggtgagg atcaggtagt acgacgcctc gaggaccgcg ctcaccagga ggagcggcca
     2701 caccggggtg attgaatgcg ggcgcgcgat cagaatgacc gcacagcaga gattggtgac
     2761 gatcaacgcc caccacacca cggggcgccg gtggtgggtg gtcttgagca gcaggttcca
     2821 ggaggcgtgg atgacggcgg agctgaggag ggctccgacg aggagggggc tcacctcgga
     2881 tactcagggg gcctgtctct cgtgaacccc ggacttgcgt ccggggttat gaacacccaa
     2941 gaccaaccat cgttgcgcct ttcataaccc cgggcgcaag cccggggatg acaagaggcg
     3001 gagccccgcc ctctcacccg cggccagccg gagccgcctc acccctgaac atcgtgctga
     3061 tcacccggta cagcacgatg ccgctgatga tcgccatcac gaagcagacc cccgcttgcc
     3121 ccgaacgtcc gtagagggca ttccctgccc cgaagagcgc ggaatagacg aaggcgcatc
     3181 cggcggtcca gccgaggagc gcctggggca gtgagtcggg cgagggtggg agcttggtgc
     3241 gctcgcggac ggcacgccac ccggcccccg ctgggcgggt gagcttgtag aagttgtcga
     3301 gcgtcgcgtc gtccacgggc ttcgtcagca tcgtgacggt gatccacagg accgtcgtca
     3361 cggcgacgac gatgatcaag gtccgagcat ccgagatgtt ccacccgaag gcgtgcttgc
     3421 cgacgaacag ggtgagcgag caggcgaatg accccatcat ggccgcgatc tcactccagg
     3481 cgttgatccg ccaccagaac caccgcagca ggtagatcaa ccccgttccc gcaccgatcg
     3541 acaggagcag ctggaaccct tcactggcgg tcgagatgaa aagcgtgaag aagccgcaca
     3601 gcaccatcag cgtcgccgtg aggccccggg aaatccacag gtagtgcttc tccgatttcc
     3661 cgcgagccag gaaccgctgg tagaagtcga tcaccagata cgacgatccc cagttgaggt
     3721 gcgtctccat ggtggaccgg taggcggcga acagcgacgc gatgagcaga cccttcatcc
     3781 ccgcgggcag caaggtcagc atcgccggat aggcgaggtc gttcccgatg agccggggat
     3841 ccacgtgcgg gatggcgcgc tgaatgtcgc caagtgtcgg gaacacgagc atcgacgcaa
     3901 gggccacgat gatccagggc caggggcgca gggcgtagtg cgcgatgttg aaccagaggg
     3961 tcgcacccag cgcgtgcttc tcgttcttcg acgccagaat ccgctgcgcg atgtagctcc
     4021 cgccccccgg ctcggcaccg ggataccaca ccgaccacca ctggatggtc accgggatga
     4081 tgaacacggc caccgcgaca tcccagttcg agaaatccgg aagcagcgaa aggacgtgcg
     4141 ggtcggtgcg gtgcaacagg ttggcgagcc cgccgacctg cggctgatcg agcgccacca
     4201 cagccgccgc gatcacgccg aacatcgcga gcgcgaactg gatcatgtcg gtcaccagca
     4261 cgccccagag ccccgagacc gaggcaaaga gtatgcacgt gagcccgccg acgatgatgg
     4321 tggtcagccg ctcccagccc agcatgacgt tcgcgatctt cacggccgcg agcgtcactg
     4381 tcgtcatgat cgtcaggttg aagaacaggc ccaggtagat cgcgcgaaaa ccgcgcacca
     4441 cggcggcggg cttccccgag tacctcagct catagaactg cagatcggtc aggacgccgg
     4501 agcgccgcca caacttggcg tagaagaaca cggtggccat tcccgtgagc agaaatgacc
     4561 accaggccca gttgtacgag acgccgtgcg atcggacgaa gtcggtgacg aggttcggcg
     4621 tgtcggtgga gaacgtcgtc gccaccatcg aggtgccgac cagccaccac ggggccgcct
     4681 gccccgacgc gaagaactca gcggtgccct tccccgaccg gcgggcgaag aagagcgcgg
     4741 gcgcgaagca gatcacgatc gagacgatcg cgatcacgac gtcgaggcgg gagagttcca
     4801 tgggggccca agatagcgcc cctcgtcagt cgtgccactc cgcgcggtac aaggtggcgt
     4861 accgcgtctt gagaacctcg agcttcggga ggtcgttggt cacgatgtac gcatcactca
     4921 agtggcgctg catgaatccc tgatgctcgg cctccgccgg atagaacgcg cggaacgcca
     4981 gcacctccgt cacgatgggg tccgaataca cgtgccgccg cttgaggctc gcgatggcgt
     5041 cctccaccga ctgcttctgc gtcgaatccc ggtagaaggc gatggagcgg tagtcgctcc
     5101 cgacgtcggg accctggcgg ttcagctgcg tgggatcatg cgccacgttg aagaagacga
     5161 gcaggagctg atcgaagctc acgcgcgccg gatcgtagat gatcatcacc gactcggcgt
     5221 ggcccgtccc gccgttgtag acctgggccg aagtcgggtt cgcgagcgtc ccgcccgcgt
     5281 accccgacac gaccttcacc acgcccttta catgctcgaa gatgccctcg acgccccaga
     5341 aacagccgcc cgcgaggacg gcagtatcga gtggggcggc ctttggcgcc gacgagggtt
     5401 gggtggagag cagcgcagcg agcagaggaa cgacggcggt catgagatct ccagtcagga
     5461 tatcacatcc tacccgacca agacggggag cgggagccga ttcgtcgctc gagacccgcc
     5521 gttcggcgcg ctacttccag gaatcgcgat acagggcggg aaaagcttcc ttcaaatgcg
     5581 ccaccttcgg cgcgtcattg taggcgatgt atggctgatc ggggtgatgc tccatgtagt
     5641 gctggtggta tccctccgct tcgtaaaacg tcgcgagcgg gatcacctcg gtgactacgg
     5701 gcttcgaata cagacgccgc ttctggagct cggagatgaa cgtgttgatc gcggcgtgct
     5761 gggtcgaatc ccggtagaac gcgatcgagc ggtagttcgt cccgacatcc gggccctgcc
     5821 ggtcgagctc cgtcggatcg tgcgccacgg tgaagaacac cgccagcagc tgcccatacg
     5881 acacctgcgc ggggtcatac accacccgca cggcctccgc gtgacccgtc tcgccggacg
     5941 agacctgctc atacgacggg gacgcggtgt gcccgcccgc atatcccgcg gtggccgaga
     6001 tgaccccacg ggtatgctcg aacacgcctt cgatgcccca gaagcagcca cccgcgaaca
     6061 ccgcggtgtc gcgtgccgga gcaagagccg tcgtgagcgc aatgagcagc ggggtgatca
     6121 tcgggtctcc ttcatgttcg tgcgccggtg cgcagcgaaa aaggtccaga gtgtccggga
     6181 gccgtcgaga ttccgtgatg agatgccgat catccgtttc ggcaggtatt gcggtcccga
     6241 aggccacgta tgcccgccat gcaccaccgt gatgccgacg acgtcagcgc ccgacggaca
     6301 gggatacact ctttcgttga tcgtggtccc atcccgggcc gtgtccggca agacgatggc
     6361 cttgaacctc tccgcgcacc cgttccggtg cacccactca tcgaggctcg ggatcgcgcc
     6421 gagaaccgtt ccccgaccgc cgccgagtgt cccgccctga tagggcacga gcggatcctc
     6481 ggtaccgttc atcgtcatca ctggaacgga gcgggccgcg tgacagttcc cggcgaggga
     6541 atctccgagg gttgcggcga cgatgccggc tgccgcgatg agatcggacg catcgcaaat
     6601 caggcgttcc gtcatgaacc cgccgttcga catcccggcc acgtagatcc ggctcgagtc
     6661 cacgggggca tgggccgcca catccgcgac cagcgcccgg aaaaaggcga cgtcgtcgat
     6721 gcccgccttg tcggccggtg tcccgagccg cccatcggcc caggagcgcc ggtacccatc
     6781 cgggtaggcg gcgatcgccc cgacggaatc ggcgatcgga tcgaagtatg agagccgcgc
     6841 ttcccccgcg ccgtccccga gccgcccgtg aagcagaacg accaatggcc gcgctccgct
     6901 cgcgttcggc gggacgtgga gcaggtacgt ccggtcgcgg cctcccaccc ggatatgctc
     6961 cgtccggtca ccgccttggt ggaagccgag acccgcggcc agacagaacg cgaccccgac
     7021 gagcgccctg gaggtcaccc gatcttccac ttggtgcccc ccggcgtgtc ctcgatctcc
     7081 acgcccttct ccttcagctc ggcgcggatc cggtccgcct ccttgaaatc ccgggactga
     7141 cgggcctggg tgcgtgccga cacgcgctcc tccacccact gcgaaaggtc ggcggcgacc
     7201 gtcttcttcg tgggcatgac gtcgagcacg tccatcacct tccggagaga tgcctgcgcg
     7261 gtgggcgcgc cgccggcacc cgcatcgatc tcgcggttcg ccgcgcgaac gaactcgaag
     7321 agatgggcca gggcctgtgg cgcgttgaga tcgtcatcca gcgctgcccg gacgttctgc
     7381 tccatgccgg cggcgaggtc ggcgaggggg cctttcggat cggggcgcgc cgtggcgagg
     7441 cgctcctgga actcaccgag ccggcgcacg ccttcggcgg ccgcggccag cgcgtcgtca
     7501 ttgaaatcga tggactggcg gtagtgtgtc tggaagaaga ggaggcgcac cgaggcggcg
     7561 tccacgccct gctccttgag gtcccgcgcc gtgaggatgt tgccataccg cttggacatc
     7621 ttggtgccgc gcaggttgag aaattctccg tgcagccagg tccgggcgaa ggtctggccc
     7681 gtcgcggcct cggactgcgc gatctcatcc tcgtggtgcg ggaagatcag gtcgatgccg
     7741 ccggcgtgaa ggtccagagt ttcgaccttg aagcgcttcc cgatctcgtc gagggacatg
     7801 gccgagcact cgagatgcca acccggccgg ccccggccga acgacgcgtc ccatgccgcc
     7861 cccacccgct cgtcctcgtc gtccgacttc ttccagaggg cgaagtcccg cgggtcgtcc
     7921 ttcgcgtatt catcggaagc gacgcgtgcc ccgactttga tctcccgcgt gtcgagacgg
     7981 gacaggcggc cgtacgtcgg gaacttgctg atggcgaagt acaccgaccc gtcgtcgccc
     8041 ttgtaggcga cgcccttggc cagaagcttc tcgatgagcg cgaccatcgc cggcacggac
     8101 tgcgtggccc gcggatacac gtgcgcgggc cggatgcgca ggaagtcccg gtccgtgaag
     8161 aacgcctgcg cgtactgctc cgtgtggtcg gtgagggtct tccccgccgc cgccgccgcc
     8221 ttgatggtcc ggtcatccac gtcggtcagg ttcatgatgt ggaagacgtc gtacccggaa
     8281 gcctcgaggt gccgccggag caggtcctcg aagagaaagg tccggaagtt tccgatgtgc
     8341 gcgtagttcc agacggtcgg gccgcacgta tacagcgtca cccgcggcgg gtggagcggc
     8401 tgaaaaggct cgatggcgcg cgtgagcgtg ttatggagga agagggccat gcggagaaaa
     8461 ttaactcacc gc
//
LOCUS       DASUBP010000095         6394 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_84493, whole genome shotgun sequence.
ACCESSION   DASUBP010000095 DASUBP010000000
VERSION     DASUBP010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6394)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6394)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6394
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84493"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..879
                     /locus_tag="VFU75_03605"
     CDS             73..879
                     /locus_tag="VFU75_03605"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5465947.1"
                     /translation="MLVLGAIPDVQYHKLRRAAAGQFSFVRALEWGQVLDTIRERPVE
                     LAVLDPLLGGEPRSHEIERLRQFFPSLPLILYTTLTPELAAVLLTLGKRGIREVIFAR
                     LDDHPTRLREALEQEYALASSQQFLEQIESRLSPLPTELRWVLEEALRMPEEVQTVQH
                     LALRASVDRRTCERWFTRAHLPSPRHFLAAARVLYAHRLLRDPGFTIEDVAKRLGYTR
                     VKTLQQHARTYLGLTAGEMRLSLTTDEAMETVVRRFLANGSSDGLKEATG"
     gene            876..1286
                     /locus_tag="VFU75_03610"
     CDS             876..1286
                     /locus_tag="VFU75_03610"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5465948.1"
                     /translation="MTGESASKMTVLVVDDEAPLRRVLERALSRLGYRVIGAASAESA
                     LELLAVERPTGILLDVHLPTMSGLAFYVAAIARWPELQGCVAVMTGDGETTEVLEWIA
                     RNSCPLIAKPFNLVQIEEWLSHVRAWRSRRSEQA"
     gene            1288..1926
                     /locus_tag="VFU75_03615"
     CDS             1288..1926
                     /locus_tag="VFU75_03615"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU5465949.1"
                     /translation="MTAPQSADPLHTLREYRSLAPWNLRDLAVLVGAVLDASAVTPIN
                     AAARAAPSERTVRFYVTRGLVSPPEGRGTAATYSYRHFLQLLWIKLRQMEGATLAAIA
                     REMVDQTGDVLERRAAQTLGAALPLPDRLALRGAPRMPRGRSGRALSAWLARDAGKDP
                     EGTRTWRRVPVTRGVELHVDSEHPLARVGADDAALAEAIRAALAKLLPPSPA"
     gene            1954..2538
                     /locus_tag="VFU75_03620"
     CDS             1954..2538
                     /locus_tag="VFU75_03620"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU5465950.1"
                     /translation="MGSWIPLILLVVVVLWAISAFNGLVSLKNQTVNAFKQIDVQLKR
                     RHDLIPNLINSVKGAMDFEKSTLEAVVQARNQAVQVNAAGPDAVKQISQAENALSGAL
                     SKLMVVVERYPDLKATGNVGQLQEELTSTENRISFARQLYNDTATQYNTKQQQFPASL
                     MAGLAHAAPAQLWEIDDQADRAVPQVDLSMKPKA"
     gene            2535..3572
                     /locus_tag="VFU75_03625"
     CDS             2535..3572
                     /locus_tag="VFU75_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU5465951.1"
                     /translation="MSGEAAPNLFQQQDANRRRTTLLVVCFVLFFAWLGFGGDYIYYL
                     ATLHNLPYEPHHTFPWFGIVLTFVGTWIAWYGYSTGPEKVLWSTGAHEITVPANDQEK
                     QLVNVVEEMAIAAGIPRPRIWVVDDPAPNAFATGTDPQHAHIAVTQGLLDLMSRDELQ
                     GVVAHELGHIKNLDVRLMTTLAALVGVVLLIRDGTGRFFWGGRGGGRSRDKGGNLGVL
                     VVVLLVIWILSWILAPIITQMLAMAVSRKREYLADAMSAQFTRNPAALASALTKIEDS
                     DAPATHLKAGAAHLCICDPLDRKLNDHPGTLGDVMASHPPIEMRIIRLKGMGYAQAKA
                     EGHLPPEAPTA"
     gene            complement(3573..4490)
                     /locus_tag="VFU75_03630"
     CDS             complement(3573..4490)
                     /locus_tag="VFU75_03630"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEU5465952.1"
                     /translation="MLGFAGVGIALKALAGFPWEDTLATLINANYWILGLALVINLGS
                     LVAKGWAWHLLLNPVSPNHWKTAQEANLVGAAVNNVSIAVGGEAARIHYIAQRDGVPV
                     GAAISSVVWTRVTETIALAIFLVMAPSLLRLPHWLRGLQVGAGLGLVIVMLITWHRGW
                     SWILNQLPASLQRRAAVLAEMGSGGRLFWPTVLGLANWGTQWATYHLVLVAAHIPVTP
                     AASFTALVAANLGGIIRITPAGLGVTQAAMVVGLLQFGVPADRSVAAGLALQALQVLP
                     VIFLGALLTGWRLTETARDAEETEALQPV"
     gene            complement(4517..5212)
                     /locus_tag="VFU75_03635"
     CDS             complement(4517..5212)
                     /locus_tag="VFU75_03635"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEU5465953.1"
                     /translation="MIETVAVVGLGAAGLAAYGAYAPNSGLYGRSVSRGPSGVPRVYL
                     TFDDGPSPSATASILDTLEKEGVPAAFFMVGDFVRREGALARAVAEAGHEAGNHTQTH
                     VKLHRRGPKRIRRELEEAHGVISEAVGRPPRAFRAPHGYRNPFVGRTVRRLKYTLFGW
                     TFGVWDSARPGAEEIRRRVRRRLVPGAVILLHDGDGYDFHGDRTQTAEALPGIIRDAR
                     DAGFVFAPLGELI"
     gene            complement(5209..6315)
                     /locus_tag="VFU75_03640"
     CDS             complement(5209..6315)
                     /locus_tag="VFU75_03640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5465954.1"
                     /translation="MRIALVTEFYYPHLGGVTEHVHNLALEFRARGHHAIVITSNMDG
                     QGPDPDWVRRVGTSRVVLSNGSYARVSTGWAITRKIVDILKAERIDIVHSQDVLAPML
                     GLKAASAGWALGIPVVATSHTWFSSSLLYRMFRWWLQPRLDRVAARIAVSDPVVHAMG
                     MYFKADWEVIPNGVDIRYFHPNGRREREAATRGPRLLFLGRLDPRNGLDTLLEAMPMI
                     LERHPTAQLIVVGDGPLRPSYEASAAHLGAAVKFVGQVSDERPDYYGGADLYLCPTTK
                     ASFGITLLEAMACGTPLVVSDITGFRELIAGGAEAVLVPKDNPLAWAHATNALIEDPA
                     RRAVMGAAGLAKAAQFAWPRIAERVLEVYQRVQK"
BASE COUNT         1082 a         2215 c         2112 g          985 t
ORIGIN      
        1 gacaacctgc gaacggaggc gctccggttt aaggtcggtt ttgcggcacg ataggacgtt
       61 tttgcgacat ttatgctcgt cctgggcgcc atacccgacg tgcagtacca caagctgcgc
      121 cgcgccgccg cgggacagtt ctccttcgtg cgcgccctgg agtggggtca ggtcctcgac
      181 accatccggg agcgccccgt cgagctcgcg gtcctcgatc cgttgctcgg cggcgagccg
      241 cgcagccacg agatcgagcg gctccgccag ttcttcccgt cgctcccgct cattttgtac
      301 acgacgctca cccccgagct cgccgcggtg ctgctcaccc tcggcaaacg ggggatccgc
      361 gaagtcattt tcgcccgcct cgatgatcac ccgactcggc ttcgcgaagc gctcgagcag
      421 gaatacgcgc tcgcgtcgtc ccagcagttc ctcgagcaaa tcgaaagccg gctgtcgccc
      481 cttcccaccg agctgcggtg ggtcctggaa gaggcgctcc ggatgccgga agaggtgcaa
      541 accgtccagc acctggctct gcgggcatcc gtggatcgcc gcacctgtga gcgctggttc
      601 actcgcgcac atctgcccag cccccgccat ttcctggcgg cggcccgcgt gctctacgcc
      661 catcgcctgc tccgggaccc ggggttcacg atcgaggacg tcgccaagcg gctggggtac
      721 acgcgcgtga agaccctgca acagcacgcg cggacctacc tcgggttgac ggccggtgag
      781 atgcgccttt cgctgacgac ggacgaggcc atggaaaccg tggtgcgccg gtttctggcg
      841 aacggcagct ccgacggcct gaaggaggcg acgggatgac gggcgaatcg gcctccaaga
      901 tgaccgtgct ggtggtcgat gacgaggcgc cgttgcgccg ggtcctcgag cgcgccctct
      961 cccgtctcgg ctaccgcgtg atcggtgcgg cgtcggcgga gagtgcactg gagctcctcg
     1021 ccgtcgagcg ccccacaggg atcctgctcg acgtccacct gcccacgatg tccgggctgg
     1081 cgttctacgt tgcggccatc gcccgttggc ccgagcttca gggatgcgtc gcggtgatga
     1141 ccggagacgg agagacgacc gaggtattgg agtggatcgc gcggaactcc tgccccctca
     1201 tcgccaagcc gttcaacctc gtgcagatcg aggagtggct gagccacgtg cgcgcctggc
     1261 gaagccgccg gtccgagcaa gcctgacgtg accgcacccc aatccgccga tccccttcac
     1321 acgctgcgcg agtaccggtc gctcgcgccg tggaacctgc gggatctcgc cgtgctggtc
     1381 ggcgccgtcc tcgacgcctc cgccgtcacg ccgatcaatg ccgccgcgcg cgcggcgccg
     1441 agcgagcgta ccgtccggtt ctatgtgacg cgcgggttgg tgagtccgcc ggagggccgc
     1501 ggcaccgcgg cgacctactc gtaccgccac ttcctgcagc tgctctggat caagctccgg
     1561 cagatggagg gagcgacgct cgcggcgatc gcgcgcgaga tggtggacca gaccggagac
     1621 gtcctcgagc gccgggccgc gcagacgctc ggcgccgcgc tccccctccc ggaccggctc
     1681 gcgctccgcg gagctccccg aatgccccgg ggccgatccg gacgcgccct ctccgcatgg
     1741 ctcgcgcggg acgcggggaa ggaccctgag ggcacccgga cctggcggcg cgtccccgtg
     1801 acgcgcgggg tggagctgca cgtcgactcg gagcatcccc tggcccgtgt gggcgccgat
     1861 gacgccgcac tggccgaagc gatccgcgcc gcgctggcga aactcctgcc gccctccccg
     1921 gcgtagggcc ctgacccgca acggagacga acgatgggtt cctggattcc cctgattctt
     1981 ctggtagtcg tcgtcctctg ggccatctcc gcattcaatg gcctcgtgtc tctgaaaaat
     2041 cagaccgtca atgccttcaa gcagatcgac gtccagctca agcgccgcca tgatctgatt
     2101 cccaacctga tcaactcggt gaagggcgcg atggacttcg agaagtcgac cctcgaggcg
     2161 gtggtgcaag cccgcaatca ggcggtgcag gtgaacgccg ccggcccgga cgccgtgaag
     2221 cagatcagcc aggccgagaa cgccctgtcg ggtgcactct cgaagttgat ggtggtcgtg
     2281 gagcggtatc ccgacctgaa ggcgacgggg aacgtcggcc agctgcagga agagctgacc
     2341 tcgacggaga accggatctc gttcgcgcgc cagctctaca acgacaccgc cacgcagtac
     2401 aacacaaagc agcagcagtt cccggcgagt ctgatggcag ggttggccca cgcggcgccc
     2461 gcacagctct gggagatcga cgaccaggcc gaccgggccg tgccgcaggt cgatctctcg
     2521 atgaagccca aggcgtgagc ggcgaagccg ccccgaacct cttccaacag caggacgcca
     2581 accgtcgccg gaccaccctg ctggtggtct gcttcgtgct cttcttcgcg tggctgggct
     2641 tcggcgggga ctacatctat tacctcgcca cgctccacaa cctgccgtac gagccgcacc
     2701 acacgtttcc gtggttcggc atcgtcctga cgttcgtcgg cacctggatc gcgtggtacg
     2761 ggtacagcac ggggccggag aaggtgctct ggtccacggg cgcccacgaa atcacggtcc
     2821 ccgccaacga ccaggaaaaa cagctcgtca acgtcgtcga ggaaatggcc atcgcggccg
     2881 gaattccgcg tccccgcatc tgggtcgtgg acgaccccgc cccgaacgcg ttcgccaccg
     2941 ggaccgatcc ccagcacgcg cacatcgcgg tgacgcaggg gctcctcgac ctgatgtcgc
     3001 gagacgagct gcaaggcgtc gtcgcccacg agctcgggca catcaagaat cttgacgttc
     3061 ggttgatgac gacgctcgcg gccctggtgg gcgtggtgct gctgatccgg gacggcacgg
     3121 gccgcttttt ctggggtggg cgcggaggtg ggcggtcacg cgacaaaggc ggcaacctcg
     3181 gcgtcctggt cgtcgtcctg ctcgtgatct ggattctgtc gtggatcctg gcgcccatca
     3241 tcacccagat gctcgccatg gcggtcagtc gaaagcggga atacctggcg gatgcgatgt
     3301 cggcgcaatt cacgcggaat ccggccgcgc tcgccagcgc cctgaccaag atcgaagact
     3361 cggatgcccc cgcaacgcac ctcaaggccg gcgccgccca cctctgcatt tgtgatccgc
     3421 tggaccggaa gctgaacgat cacccgggga cgttgggtga cgtgatggcc tctcatccgc
     3481 cgatcgagat gcggatcatc cggctcaagg ggatgggata cgcgcaggcg aaagccgagg
     3541 gacacctgcc ccccgaggct cccaccgcct gatcaaaccg gctgcagcgc ttcggtctct
     3601 tccgcgtcgc gcgcggtttc ggtgagacgc cagccggtga ggagagcgcc gaggaaaatt
     3661 acggggagca cctggagcgc ctggagcgcc agccccgccg ccaccgaccg gtccgccggc
     3721 actccgaact gcagcagacc gactaccatc gccgcctgtg tcacgccgag tcccgcgggc
     3781 gtaatccgga tgataccgcc cagattcgcg gcgaccagcg ccgtgaacga agcggccgga
     3841 gtaaccggga tgtgcgcggc cacgagcacc aggtgatagg tggcccactg cgtgccccag
     3901 ttggcaagac ccagtaccgt cggccagaag aggcgtcccc cggaacccat ctccgccagg
     3961 accgccgccc gccgctgcaa ggaagccggc agctgattca agatccacga ccagccgcgg
     4021 tgccaggtga tcagcatgac gatcacgagc ccgagcccgg cgcccacctg caagccgcgc
     4081 agccaatgcg gcagacgcag gaggctcggc gccatcacga ggaagatggc gagcgcgatg
     4141 gtttcggtga cgcgggtcca gacgacggag gagatggcgg cgccgaccgg cacgccgtca
     4201 cgttgggcga tgtagtgaat gcgcgccgct tcgccgccga cggcgatcga cacgttgttc
     4261 accgccgcgc caaccagatt cgcctcctgc gcggtcttcc agtgattggg agagacggga
     4321 ttgagcagca gatgccacgc ccagcccttg gccacgagcg agccaaggtt gatcaccagg
     4381 gcgaggccaa gaatccagta gttggcgttg atgagggtcg ccagcgtgtc ttcccagggg
     4441 aacccggcca gcgccttcag cgcgatgccg acccccgcga atcccagcac ccaccagatc
     4501 caccgacggc ttttcttcag atcagctccc cgagtggtgc gaagacgaac cccgcgtcac
     4561 gggcgtcgcg gatgattccc ggcaacgcct cggcggtctg cgtgcgatcg ccgtggaaat
     4621 cgtatccatc gccgtcgtgg aggagaatca ccgctcccgg cacgaggcgc cgtctcaccc
     4681 gccggcgaat ctcctcggcg ccgggacgcg cactgtccca gaccccgaaa gtccagccga
     4741 acagggtgta tttgagtcgc cgtacggtac ggcctacaaa tgggttccgg tagccgtggg
     4801 gggcccggaa ggcccgaggg ggcctcccca ccgcctcaga gataacgcca tgcgcttcct
     4861 cgagttcccg ccggatccgt ttcggccccc gacggtgcag tttgacatgg gtctgggtgt
     4921 ggtttcccgc ctcgtgcccc gcctcggcga ctgcccgtgc caacgctcct tcgcgccgga
     4981 cgaagtctcc caccatgaag aaggcggccg ggaccccctc cttctccaag gtgtcgagga
     5041 tgcttgcggt ggcactcggg gagggaccgt catcgaaggt gaggtagacg cggggaacac
     5101 cgctcggccc ccgggagacc gacctgccat acagcccgct gttcggcgcg taggctccgt
     5161 aggcggcaag gcctgccgcg cccaacccaa ctacggcgac cgtctcgatc acttctggac
     5221 ccgctggtag acctcgagca cgcgctccgc aatccggggc caggcgaact gcgccgcctt
     5281 ggcgaggccc gcggctccca tcactgcgcg acgggccggg tcttcgatca gcgcgttggt
     5341 cgcgtgggcc caggcgagcg ggttgtcctt cgggaccagc accgcctcgg cgcctcccgc
     5401 gatgagctcg cggaagcccg tgatgtcgga gaccacgagc ggggtaccgc acgccatggc
     5461 ttccagcagg gtgatgccga acgacgcctt cgtggtgggg cacagataga gatcggcccc
     5521 cccgtagtag tcggggcgct cgtccgacac ctgccccacg aacttcaccg cggccccgag
     5581 atgggcagca ctcgcttcgt agctcgggcg caggggcccg tcgccgacca cgatcaactg
     5641 ggccgtcggg tgacgctcga gaatcatcgg catcgcttcg agcaacgtgt cgaggccgtt
     5701 ccggggatcg agccgtccga ggaagagcag ccgcggcccc cgggtcgcgg cttcacgctc
     5761 gcggcggcca ttggggtgga aatagcggat gtccacgccg ttggggatga cctcccagtc
     5821 ggccttgaag tacatcccca tcgcgtgaac cacaggatcc gacaccgcaa tccgcgccgc
     5881 gacccgatcg agccgcggct gcagccacca ccggaacatc cgatacagga gggagctcga
     5941 gaaccacgtg tgggatgtgg cgacgaccgg gattccgagc gcccagccgg cgctcgccgc
     6001 cttgagaccc agcatgggcg cgagcacgtc ctggctgtgg acgatgtcaa tccgctcggc
     6061 cttcaggatg tcgacgatct tgcgggtgat ggcccacccg gtggagaccc gggcgtacga
     6121 gccgttcgaa agcaccaccc gactggtgcc cacccgccgg acccagtcag gatcgggacc
     6181 ctgcccatcc atgttcgagg tgatgacgat ggcgtggtgg ccccgggccc ggaattcgag
     6241 cgccaggttg tgaacgtgtt cagtgacccc gcccaagtgg gggtagtaga actccgtgac
     6301 cagagcgatg cgcatgtgag cgaagggctc ccggggcggg ggcaagccaa caacgtaatg
     6361 tgcgccccga gcaaagcgag gggtgcaccc tgag
//
LOCUS       DASUBP010000096         4388 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_956583, whole genome shotgun sequence.
ACCESSION   DASUBP010000096 DASUBP010000000
VERSION     DASUBP010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4388)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4388)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4388
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_956583"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VFU75_03645"
     CDS             complement(<1..554)
                     /locus_tag="VFU75_03645"
                     /inference="COORDINATES: protein motif:HMM:NF025343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5465955.1"
                     /translation="MTKRPKPAPDDGLRRTRERLLHATVELMAEIGVDRVRTRSIAER
                     AGVNPALVHYHFGTVSALVMEAAQDALLRTLGPSLEALQSGTTIRDSMRGILRWLERH
                     GQDAPGSTILAEAMVKATRSPSFRRWARQASRRFRLVILERLRAARKAGELDPELDVA
                     AAATLLAAALDGLLFHRLVDPKLDV"
     gene            complement(592..1056)
                     /locus_tag="VFU75_03650"
     CDS             complement(592..1056)
                     /locus_tag="VFU75_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465956.1"
                     /translation="MHPRFIVFASFASAVALAACSSSSTGLAAGPVGNWHVTVSQLAG
                     SGIYTYDIRPAPFVLTISQSSATYTATFPALFGITTSSGDTVHRWSQNEGASLVVITA
                     DSLRMSLDDTLHTCTLTLVGAINGTSASGSGILGNCDGPGTPTPGSWSATKF"
     gene            complement(1190..3646)
                     /locus_tag="VFU75_03655"
     CDS             complement(1190..3646)
                     /locus_tag="VFU75_03655"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5465957.1"
                     /translation="MNLLSQHVRYGARALARSPIFALVAVLSVAIGAGATTGVVTLVD
                     TMLLRPPSGVEHAERLVVVGRTQNGKGFDNFSYPAFAAYRTARSLSGLAATEIGPQTV
                     SLAGPSGGEPMHVSTVSGNLFDVLEARTFLGRFFAKDEDGAPGEKPVLVLSYAYWQRR
                     FGADPSILGRPLVLNGAPFTVIGIAAKGFQGPFATAPDGWVPLAAVTYFGTPASMFNE
                     PSGVWLMAVGRLAPNASLDQAQAELAGITARLARDNPNAYEGKGVTVERASLFPGDMQ
                     AAIGGFLALLFVVAGLVLVIASTNVAGMLLARAAARQREIAVRLALGASRAQLVWQLV
                     IECLLLFGAAGALGLVIANWMVRGLLELIPRLPVSLSLDPRLDWRVLTAALVLALGTG
                     LLAGLVPALQSTRPNLVPALKAGSGGSDGAGRLRLRSGLLVVQIAFSMVLLVVAGLFA
                     RTLQHARGIDPGFDPEGVAFTTLDFGTANYSEDRGRQAATAILNQVRALPGVHGAALS
                     AMLPLGGGGLGFGPITVPGRPVDPREGELDADWDVVSPGYFATLGIPLVQGRDFSEAD
                     RAGVGDVAIINEAYAAALFPGEDPVGRTLVNEGRTASDSRTLTVIGVVRNAKYRFLGE
                     AQRNFIYVPLSQRYFARTNVLVKMTPGVAMPAVAVGRIVAAVDPALPILRQEALRDQM
                     AVALLPQQLALFVSGGLGLVALLLALLGIYGVTAYGVSQRTREIGVRIALGAPRGHVL
                     GLVLRQGLVLAGVGVLLGALAAFGATGLLRGLLYGVAPADVVALGGAAVLLALAALAA
                     SWVPARRAARLDPVIALRSE"
     gene            3805..4011
                     /locus_tag="VFU75_03660"
     CDS             3805..4011
                     /locus_tag="VFU75_03660"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HEU5465958.1"
                     /translation="MRTTLILDDALLDRARELSGIQEKTALVRAGLEALITREAARRL
                     AALGGSMPNLAPTPRRRPGSKRGA"
     gene            4008..4373
                     /locus_tag="VFU75_03665"
     CDS             4008..4373
                     /locus_tag="VFU75_03665"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HEU5465959.1"
                     /translation="MIILADTSVWIDHWRRGSPRFQQLLEADQIVVHPFVFGEIALGA
                     ITPRAEVLTHLGALRAPRVAQHAEVMALIDRARLWRRGIGWVDVHLLASALLDQIRLW
                     TLDQPLARTARALGVALEV"
BASE COUNT          765 a         1564 c         1443 g          616 t
ORIGIN      
        1 acgtcgagtt tgggatccac caggcggtgg aagagcaggc cgtccagggc ggcggcgagg
       61 agggtggcgg cggcggccac gtcgagctcc ggatcgagct cgcccgcctt tcgggcggcc
      121 cgcagacgtt cgaggatgac cagccggaag cggcgtgagg cctggcgggc ccagcggcgg
      181 aaggacgggc tgcgggtggc cttcaccatg gcttccgcga ggatggtgga gcccggggcg
      241 tcctgcccgt ggcgctccag ccaccgcagg atgccgcgca tggagtcgcg gatggtggta
      301 cccgactgca gggcttcgag ggagggaccc agggtgcgca gcagggcgtc ctgggcggcc
      361 tccatgacga gcgccgacac cgtgccgaag tgatagtgga cgagcgccgg gttcaccccg
      421 gcccgctcgg cgatggaccg cgtccgcacg cggtcgacgc cgatctcggc catcagctcg
      481 acggtcgcgt ggagcagccg ttcccgggtg cgtcggagtc cgtcgtccgg ggccggtttt
      541 ggccgtttgg tcatttgact aaatcaaatg accaactagg ccgatgtcaa ctcagaactt
      601 cgtcgcgctc catgacccag gagtcggcgt ccccggtccg tcacagttgc ccaggatgcc
      661 gctgccggag gcggaggtcc cgttgatcgc gccgaccaat gtgagagtgc aggtgtggag
      721 agtgtcgtcc agtgacatcc ggagggaatc cgcggtgatc acgacgagcg acgcaccctc
      781 gttctgagac cagcggtgca ccgtgtcccc cgagctggtc gtgatcccga acagtgccgg
      841 aaatgtcgcc gtgtaggtgg cgctgctttg gctgatcgtc agaacaaatg gcgccgggcg
      901 gatgtcatac gtgtagatgc cgctacccgc cagctgactt acggtcacgt gccagtttcc
      961 gaccggaccc gcggccagtc cggtactcga acttgagcag gccgccaggg caacggcgga
     1021 ggcaaaggac gcgaatacga tgaaccgagg gtgcatggtg cttcctcatc gctcagggga
     1081 aacaccacgc taatggctag ccgtcctcca gccgttacct ggtgggtgac ggctctatct
     1141 agagtgctga cagtcgtttc aagatgctgg cgacggggct gccgccaccc tactcgctcc
     1201 gcagggcaat caccggatcg agccgcgcgg cgcgccgggc gggcacccag ctcgccgcca
     1261 acgccgccag cgccagcaac accgccgcgc ctcccaatgc caccacatcc gccggcgcca
     1321 cgccgtacag caagccccgc aacagccccg tcgccccgaa cgcagccagc gcgcccagca
     1381 gcaccccgac ccccgccagc accagaccct ggcggagcac caggcccagc acgtgtccgc
     1441 gcggcgcacc caacgcgatc cgcacgccga tctcgcgcgt tcgctgcgac acgccgtacg
     1501 ccgtcacgcc ataaatgccg agcagggcga gcagcagcgc gacgagcccc agaccgccgg
     1561 agacgaacag agccaactgc tgcggcagga gcgcgacggc catctggtcg cgcagcgctt
     1621 cctggcgcaa gatcgggagc gccggatcga ctgcggccac gatgcgccca accgccacgg
     1681 ccggcatcgc cactcccggc gtcatcttga ccaggacgtt cgtccgggcg aagtaccgct
     1741 ggctgagcgg cacatagatg aagttgcgtt gcgcctcgcc cagaaaacgg tacttcgcgt
     1801 tccgaaccac gccgatcacc gtgagggtac ggctgtcgct ggccgtccgt ccctcgttga
     1861 ccagcgtccg tcccaccggg tcctcgcccg gaaagagcgc cgcggcatag gcctcattga
     1921 tgatcgcgac gtcgcccacc cccgcccgat ccgcctccga aaaatcacgc ccctgaacca
     1981 gcggtatgcc gagcgtggcg aagtacccgg ggctcacgac gtcccagtcg gcgtccagtt
     2041 ccccctcgcg cgggtccacc ggccgcccgg gaaccgtgat gggaccgaac cccagcccgc
     2101 cgccgccgag cggcaacatg gccgaaagcg cggcaccatg gactcccggc agggcacgca
     2161 cctggttgag aatcgccgtc gcagcttgcc ggcccctgtc ctcactgtag ttcgcagtcc
     2221 cgaaatcgag cgtcgtgaag gcgacgccct ccggatcgaa tccgggatcg atgccccggg
     2281 catgctgcag cgtacgggcg aacaggccgg cgaccaccaa gagcaccatc gagaacgcga
     2341 tctggaccac caggagaccg ctccgcaggc gcaggcggcc cgcaccgtcg cttccccccg
     2401 acccggcttt gagcgcgggc accaggttgg gtcgggtgga ctgaagagcg ggcaccaggc
     2461 cggcgagcaa gcccgtcccg agcgcgagga ccagggcagc cgtgagcacg cgccagtcga
     2521 gtcgggggtc aagcgagagg gacaccggaa gccgggggat gagctcgagc aatccccgga
     2581 ccatccagtt ggcgatcacg aggcccaacg cgcccgcagc cccgaacaac aacaggcact
     2641 cgatcaccag ctgccacacg agctgggcgc gcgaggcgcc gagcgccagg cgcacggcga
     2701 tctcccgctg ccgggccgcg gcgcgggcca acagcatgcc cgccacgttg gtactcgcga
     2761 tcacgaggac gagcccggcc accacgaaga gcagcgcgag gaacccgccg atcgcggcct
     2821 gcatgtcccc cggaaagaga ctcgcccgtt ccaccgtgac acccttgccc tcatacgcat
     2881 tcggattgtc gcgggccaga cgcgcggtga tgccggccag ctcggcctgc gcttgatcga
     2941 gactcgcatt gggggcgagc cgccccacgg ccatgagcca aaccccggac ggctcgttga
     3001 acatggacgc gggcgtgccg aagtaggtga cggccgcgag cggcacccag ccgtcaggcg
     3061 ccgtggcgaa ggggccctgg aatcccttgg cggcgatccc tatcaccgtg aacggtgccc
     3121 cattcaacac gagaggccgg cccagaatcg atgggtcggc cccgaaacgg cgctgccagt
     3181 atgcatagct gaggacgagg acgggcttct cccccggcgc cccatcttcg tccttcgcga
     3241 agaaccgtcc caggaatgtc ctcgcctcca ggacgtcgaa cagattcccg ctgacggtgg
     3301 agacgtgcat cggctcgccc ccgctcggtc cggccaggct gaccgtctgc ggcccgatct
     3361 cggtggccgc gagtccactc agcgagcggg cggtgcggta agccgcgaac gccgggtacg
     3421 agaagttgtc gaagcccttc ccgttctgcg tccgcccgac gacgacgagc cgctcggcgt
     3481 gttctacgcc ggagggcggg cgaagcagca tggtgtcgac gagcgtgacg acgcccgtgg
     3541 tcgcaccggc gccgatcgct acggacaaca cggcgaccag tgcgaagatc ggactccggg
     3601 cgagtgcccg ggccccgtac cgcacgtgct gactcaagag gttcaacatc aacaggcccc
     3661 gatgggtatc cggcccctac ggtaacgatg tgagcgggtt tccgcgcgcg tcgctccccg
     3721 gcactccagc cgctgccctc gtcgcgctgc tcagttgacc cgtggttgac agactacata
     3781 tagattatgc atatctatat gcatatgagg accacactga ttctcgacga cgccctgctg
     3841 gaccgggccc gcgagctgtc cggcattcag gaaaaaaccg ccctggtgcg cgccgggctc
     3901 gaggccctca tcacccgtga agccgcccgc cggctcgccg ccctgggcgg ctccatgccc
     3961 aacctggcgc ccactccccg ccggagaccc ggcagcaaac gcggcgcgtg atcatcctcg
     4021 cggacacgtc cgtgtggatc gatcactggc gccgcggcag cccacgcttc caacagctgc
     4081 tcgaagcgga ccagatcgtg gtccatccgt tcgtcttcgg agaaatcgcc ctcggcgcca
     4141 tcaccccgcg ggctgaagtg ctcacccatt tgggggctct gcgcgctccc cgcgtggccc
     4201 agcatgccga ggtgatggcc ctgatcgatc gcgcgcgcct ctggaggcgg gggatcggct
     4261 gggtcgacgt ccatctcctc gcctcggcat tgctcgatca gatccggctc tggaccctcg
     4321 accaacccct cgcccgcacc gcgcgcgccc tcggcgtggc gttagaggtc taaggagaga
     4381 accttgaa
//
LOCUS       DASUBP010000097         4387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_670204, whole genome shotgun sequence.
ACCESSION   DASUBP010000097 DASUBP010000000
VERSION     DASUBP010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4387
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_670204"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..727
                     /locus_tag="VFU75_03670"
     CDS             <1..727
                     /locus_tag="VFU75_03670"
                     /inference="COORDINATES: protein motif:HMM:NF014307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit beta"
                     /protein_id="HEU5465960.1"
                     /translation="LSAVAMGFVLDNRLLVTAGALDGSSGLILSIIMCKAMNRSFANV
                     LFGAFGAAPKMAAAGEQKEWKQESVEGAAQILEQARMVVIIPGYGMAVAQAQHKAQEL
                     YDQLTRRGVIVRFAIHPVAGRMPGHMNVLLAEANVPYSALVEMDEINPEMPQCDVALV
                     VGANDVVNPAARTAKGSPIYGMPIIDADKARTVLAIKRSKNPGFAGIDNELYVKDNTW
                     MLFGDAKAVLGELVKQFSTSGMH"
     gene            complement(733..2610)
                     /locus_tag="VFU75_03675"
     CDS             complement(733..2610)
                     /locus_tag="VFU75_03675"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU5465961.1"
                     /translation="MLLLLVASMAIQSPQQAADSSPFRALPLPTANRMRSANGAPGPN
                     YWQQEADYTIHASVDTASSVLTGSERIHYVNHSPDSLPFVWMQIEQNIFAKNSITYQL
                     NQPPLEFAGGVSFDFTGQGFIGGITISKLESGGKALVRTEYGTMMRVELPSPLAPGAA
                     IDFDVDWRFPIPPYGGGRMGRVGNRLYELGQWYPRMVVYDDIHGWNPLPYIGAGEFYL
                     EYGNFDVTLTLPAGYVLGSTGTVANPEEVWSADTRARIEKARNSESAVGIVTKEEATA
                     NGRRPTAGTKNWHVTATNVRDFAWCASPDYRWDASGYDGKLINTLYRPEAQPWLEANR
                     MARFTIKYFSEQWFTYPWPKATTCEGPVQGMEYPMITFVPALPAREDQFWDLMHEFGH
                     QWFPMVTGTDERRYPWMDEGFNSFIDYGAAAEYFKGTAYGDTVWREVLSFAKPAMAPG
                     AEKPMIDKPVEQQNLAWAGYQKPPLMLILLRDQVLGKATFERAFRDYIRRWQFKHPQP
                     ADFFRTMSDVSGQDLDWFWREWVFTTARLDQAVDSVRAKGDSSYVYLSNREAMVMPVT
                     AELTHADGTKERVSLPIEMWNLAPRFTLRVASKSPLVSVVIDPDVLYPDRDRSNNSWK
                     K"
     gene            complement(2671..>4387)
                     /locus_tag="VFU75_03680"
     CDS             complement(2671..>4387)
                     /locus_tag="VFU75_03680"
                     /inference="COORDINATES: protein motif:HMM:NF012935.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEU5465962.1"
                     /translation="RERYLRSWALSRLNLRRSGFQALKRITAVTRYTATDERGANPTW
                     AALGYPGPASPPPATPKTIKPLVLDGDTTLDCDVVVVGSGAGGGVVAGELARAGKHVV
                     VLEKGAYLNEADFTQREGEMLVQLYDAGGLLATKDLGLVVLQGSCLGGGTVVNYTTSF
                     HTPDRIRESWAREHGLPHFTTSDFTTHLDAVAQRIHVNTEHAKPSGRDRLLIQGLERC
                     GWHHGLLPRDVDGCPQDDACGYCGYGCQRSAKRSTLVTYLQDAADRGATFVVGADVRR
                     VVRERGRATGVEALVRVPGASRPFALTVRARSVVVAGGSIHSPALLLRSGIRHPELGR
                     NLTLHPATAIFGLMDEPVRPWTGALQAHFSDQFADLDQGYGFKFETAPLHPSLAALAQ
                     PWETAATFREAMARLPNLTLVGILLRDRFGGRVRVNDAGAPVIEYALSRYDRTHLWRA
                     VQEASRLLESAGAREIFAPQARGNAYRPAEGAGARGRWLEQVHQAGLGANQMLSVTFH
                     QMGSCRMGGNRKLAVVDPENQVWDTPGLFVADASVFPSASGVNPMLTIMGIAHRAAGI
                     ISHQP"
BASE COUNT          730 a         1423 c         1484 g          750 t
ORIGIN      
        1 cctttcggcg gtcgcgatgg ggttcgtgct ggacaaccgg ctcctcgtta ccgccggcgc
       61 cctcgacggc tcgagcggcc tgattctgtc gatcatcatg tgcaaggcga tgaaccgctc
      121 cttcgcgaac gtgctcttcg gagcgttcgg ggcggccccc aagatggcgg cggccggcga
      181 gcagaaggaa tggaagcagg agtcggtgga aggggcggcg cagattctcg agcaggcgcg
      241 gatggtcgtc atcatccccg ggtacggcat ggccgtcgcc caggcccagc acaaggcgca
      301 ggagctctac gaccagctca ccaggcgcgg ggtgatcgta cggttcgcca tccacccggt
      361 ggccgggcgg atgccggggc acatgaacgt cctgctcgcg gaagccaacg tgccctattc
      421 ggccctggtc gaaatggacg agatcaaccc ggagatgccg cagtgcgacg tggcactggt
      481 cgtcggtgcc aatgacgtgg tgaatcctgc cgcccggacg gcgaaggggt cgcccatcta
      541 cggcatgccg atcatcgacg cggacaaagc ccggaccgtg ctcgccatca agcgaagcaa
      601 gaatccgggt tttgccggga tcgacaacga gctctacgtc aaagacaaca cgtggatgct
      661 cttcggagat gccaaggcgg tcctcggcga gctcgtcaag caattctcca cttcaggaat
      721 gcattaactc cttcacttct tccagctgtt gttggagcgg tcgcgatccg gatacaacac
      781 atccgggtcg atgaccacgc tcaccagtgg cgacttcgac gccacccgca gggtgaacct
      841 gggcgccagg ttccacatct cgatcggcaa gctcacccgc tccttcgtgc cgtccgcgtg
      901 cgtcagctct gcggtcaccg gcatcaccat cgcttcgcgg ttcgagaggt agacgtagct
      961 ggaatcccct ttcgctcgaa cggaatccac ggcctgatcg agccgggcgg tcgtgaagac
     1021 ccactcccgc cagaaccagt cgagatcctg gcctgagacg tcggacatcg tgcggaagaa
     1081 gtccgcgggt tgggggtgct tgaactgcca ccggcggatg taatcgcgga acgcgcgctc
     1141 gaacgtggct ttccccagga cctgatcccg caggagaatc agcatcagcg gcggcttctg
     1201 gtacccggcc cacgccaggt tctgctgctc caccggcttg tcgatcatcg gtttctcggc
     1261 gccgggagcc atcgcgggtt tggcgaatga caggacctcc cgccacacgg tgtcgccgta
     1321 cgccgtgccc ttgaagtact cggcggccgc gccgtagtcg atgaacgaat tgaatccttc
     1381 atccatccag ggataccggc gctcgtcggt gcccgtcacc atcgggaacc attggtgccc
     1441 gaactcgtgc atcagatccc agaactggtc ttcgcgcgcg gggagagcgg gcacgaaggt
     1501 gatcatcggg tactccatcc cctgaaccgg accctcgcac gtcgtcgcct tcggccaggg
     1561 atacgtgaac cactgctccg agaagtactt gatggtgaac cgcgccatcc ggttcgcctc
     1621 gagccagggt tgcgcctcgg ggcggtagag cgtgttgatc agcttgccgt cgtagcccga
     1681 ggcatcccac cggtagtcag gggacgcgca ccaggcgaag tcacggacat tggtcgcggt
     1741 gacgtgccag ttcttcgtcc cagcggtggg ccgccggccg ttggccgtcg cctcctcttt
     1801 ggtgacgatc ccgacggcgc tctcagaatt ccgggccttc tcgatccggg cgcgcgtatc
     1861 cgccgaccag acctcctcgg gattcgctac cgtgccggtg gaccccagga cgtagccggc
     1921 gggaagggtg agggtgacgt cgaagttgcc gtactcgagg tagaactctc cggcgccgat
     1981 gtagggcagg gggttccagc catggatgtc gtcgtagacc accattcgcg gataccactg
     2041 gcccagctcg tagagccggt tgccgacccg gcccatgcgg ccgccgccgt acggcggaat
     2101 cgggaagcgc caatcgacgt cgaagtcgat cgcggcaccc ggggccagcg gtgagggcag
     2161 ctccacgcgc atcatggtgc cgtactcggt ccggaccaac gctttgccgc cggactcgag
     2221 tttggagatg gtgatgccgc cgatgaatcc ctggccggtg aagtcgaagc tcacaccacc
     2281 cgcgaattcg aggggcggct ggttgagctg ataggtgatg ctgttcttcg cgaagatgtt
     2341 ctgctcgatc tgcatccaca cgaaggggag cgagtccggc gaatggttca cgtagtggat
     2401 ccgctcactg ccggtgagca cgctcgacgc ggtgtcgacg ctcgcgtgga tcgtgtagtc
     2461 ggcctcctgc tgccagtagt tcggtcccgg tgcgccgttc gccgagcgca tccggttcgc
     2521 ggtcggcagc ggcagggctc ggaaaggaga tgaatcggcg gcttgctggg gagactggat
     2581 ggccatggaa gcgacgagca agagcagcat ggtttctcct gacggttgag ccgtcagccg
     2641 gcagcgatca gcaggctgat ggctgatggc ttacggctga tggctgatga tccctgccgc
     2701 acgatgcgcg attcccatga tcgtcagcat cggattcacg cccgacgccg acgggaacac
     2761 gctggcgtcc gcgacgaaca gcccgggcgt gtcccaaacc tgattctccg ggtcgacgac
     2821 cgcgagtttc cggtttccgc ccattctgca gctgcccatc tgatggaagg tcaccgagag
     2881 catctggttc gcgccgagtc ccgcttggtg tacctgctcg agccagcgcc cccgggctcc
     2941 ggcgccctcg gccgggcggt aggcgttccc ccgggcctgc ggcgcgaaga tctcgcgggc
     3001 gccggcggac tcgagcagac gcgacgcctc ctgtacggcc cgccagaggt gcgtccggtc
     3061 gtacctcgaa agggcgtatt ctatcacggg agcgcccgcg tcgttgacgc ggacccggcc
     3121 gccgaaccgg tcgcgcagga gtatcccgac cagcgtgagg ttcgggagtc tggccatcgc
     3181 ctcccggaag gtggccgccg tttcccacgg ctgcgcgagt gcggcgagtg acggatgcag
     3241 tggtgcggtc tcgaatttga acccgtaccc ctggtcgaga tcggcgaatt ggtcactgaa
     3301 atgggcctgg agcgcgcccg tccagggccg cacgggctcg tccatcaggc cgaaaatcgc
     3361 ggtcgccggg tgcagggtga ggttccgccc caattcgggg tggcggatcc ccgagcgcag
     3421 caggagggcg ggggaatgaa tcgagccacc cgcgacgacg acggaccgcg cacgcaccgt
     3481 cagcgcgaac ggccgtgacg caccggggac gcgcaccagc gcctccactc ccgtcgcccg
     3541 cccgcgctcc cgaaccacgc gccggacgtc ggcgccgacg acgaaagttg ctccgcggtc
     3601 cgcggcatcc tgcaagtagg tgaccagcgt cgagcgcttc gcgcttcgct gacacccgta
     3661 gccgcagtat ccacaggcgt catcctgggg acagccgtcc acgtctcgtg ggagaagacc
     3721 gtggtgccag ccgcaccgct cgaggccctg aatgaggagg cggtctcgcc ccgacggctt
     3781 ggcgtgctcc gtgttcacgt gaatccgctg ggcgacggcg tcgagatgcg tggtgaaatc
     3841 actcgtcgtg aagtgaggca gtccatgttc gcgggcccag gactcgcgga tccggtcggg
     3901 tgtgtgaaag ctcgtcgtgt agttcacgac ggtgccgccg ccgaggcagc tgccctgcag
     3961 cacgacgagc cccaggtcct tggtggcgag caggccgccg gcgtcgtaga gctgcaccag
     4021 catctcgccc tcgcgctggg tgaagtcggc ctcgttgaga tacgcgccct tctcgaggac
     4081 gacgacgtgt tttcccgcgc gcgcgagctc gcccgcgacc acgcccccac ccgcgcccga
     4141 ccccacgacg actacgtcgc agtcgagggt ggtatctccg tcgagcacga ggggcttgat
     4201 cgttttcggc gtggcggggg gcggggaagc aggtcccgga tagccgaggg ccgcccaggt
     4261 gggattggcg ccacgctcat cggtggccgt gtaacgggtg acggcggtga tccgtttcag
     4321 cgcctggaat ccgctgcggc gcaggttgag gcgggagagt gcccacgagc gcaggtaccg
     4381 ctcccgg
//
LOCUS       DASUBP010000098         6893 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_779183, whole genome shotgun sequence.
ACCESSION   DASUBP010000098 DASUBP010000000
VERSION     DASUBP010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6893)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6893)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6893
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_779183"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            336..1997
                     /locus_tag="VFU75_03685"
     CDS             336..1997
                     /locus_tag="VFU75_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5465963.1"
                     /translation="MGWFGVRRTEATVPSPQTLLRAVYVGRICLAVAIYVTAAAKVAV
                     AAPLQVLVVSFVLIAAILFTAWSYWRSHFRALPPSETFLYLQAIFDAALITTVVHMTG
                     GADSDFATLYVPLIAVIAVLMPPQSTALVTALVGLVYFADIFFGHRGVIGPGLFIQLG
                     VFAAVAAVTAYFASRVKVMGAEREALAGELQQVRLEADDVLRNLRTGVVTVDSSGKLL
                     YSNPAAEALLGFSADEWRDKPIMPELARLAPEFWAAIIATARRGVRLMRVEASVRRGE
                     QVFPIGLTTTTIEGPKGEQPRVTAIFADISDSKRLQELHLRAERLEAVAELSSSLAHE
                     IKNPLASIRSSVEQLSRSSRTNPDEKFLANLIVRESDRLSRLLSEFLDFSRVRVTEYR
                     VLDLVEVAGAAARLVEEREDFPEDAQITLEGAPTPMEGDEDLVHRVVSNLVLNAAQAA
                     GKGARIEVRIGPASPADLPSGAGIENPVRVQIADNGPGIPDAVRERLFEPFVTGRVGG
                     TGLGLAIVQRAVEAHRGLVLVDSTPGGGTTFTIYFPAARQREEAA"
     gene            1994..3385
                     /locus_tag="VFU75_03690"
     CDS             1994..3385
                     /locus_tag="VFU75_03690"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5465964.1"
                     /translation="MTAPVRSSQPSILVVDDESGILQTLQILLKNEGFDVTTAQGGKA
                     GLEALKAHAPDIVLTDVRMPQVTGIDILAAVRQQDPETPVILMTAQASLQTAIQAVNE
                     GAFYYIQKPFSNDDMVAICRRAADYRNLRAENKQLKQEIRRRERSGLVKPLGKSRPFS
                     DVMRLAEQVAPTESTVLIQGESGTGKEVIARYIHELSARSEGPFLSLNCGALPESLLE
                     SELFGHVKGSFTGAVRDKQGLFAAARGGTFFLDEIGEMSPATQVKLLRVLQEREAIPV
                     GGTEAVPLDVRVVAATNRDLEEEIKRGRFRTDLFYRLNVIAIHLPPLRDRRDDIPIFV
                     DAFLKRIAKEHGEEPKSLAPDALEVIMAYDWPGNVRELENALERAVVLVKANLIPLSA
                     IPTKVTERKAEPLVTEEVRTNPTLDVIEQAYITWVLNAEGGNKTRAAEVLGIDPSTLY
                     RKLSKYEGGKEPA"
     gene            3382..4092
                     /locus_tag="VFU75_03695"
     CDS             3382..4092
                     /locus_tag="VFU75_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5465965.1"
                     /translation="MKLSIIVPAYNEEQTVETVMKKLRELKEPVEIIAVNDCSTDGTG
                     AVLDRLKKVGLVDQVIHHPQNRGKGAALRAGFAAATGEVVVVQDADLEYDPADLPALL
                     VPIKRGVADAVFGSRFQGGPHRVLYFWHSVGNSVLTLLSNMFTNLNLTDMETCYKVIR
                     ADLIKKLPLTSDRFGFEVEITARLAQAGARIWELPISYSGRTYAEGKKITWKDGVAAF
                     FHIVRYNMFPPPGEWRTP"
     gene            4077..5564
                     /locus_tag="VFU75_03700"
     CDS             4077..5564
                     /locus_tag="VFU75_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465966.1"
                     /translation="MADALTPRQAGWIAGAVALAAGLFTLNHYLTGVFYDDGLYAGLA
                     WAMAHGHGFVHPNLPGTPAAVHFPPLFPVVLIPFFATLSISAAAFAGKLLNVVLFAAG
                     SALVASYAVRTKLLGEAPPWVSVALVAVAAIAIPVLTLLGALMSEPLFALLLVGAVML
                     VDDSPLSAGVCVAAALLTRSIGVAAGAGILLYAWRRGASRRQLVEFAVPVFIAGAAWG
                     FWVLRHKAGIDPAVASDYGSYFETVKSAGLGALGSSTTDLPRPLSVLTLNWVPSRVVY
                     YALGIPALLVGLYGLALFCVRSSIGWTLVFYLAILGIWPFPPDRFLWAVLPWLGLAWG
                     AGAFGLWRRIQKWPRVALVLLAVALGGGYARYEIRGLSHRWWDLSARSISDNFAELVP
                     AIDSLPAAAVLATDNEPLVYLATGRQSVPFYLFMYQGRQVLEPTPGLQRAYLERYGVT
                     HVLLSGTGTASEGELRRLIAAYPTLLTPVRMWPGGRTLYEVRRDH"
     gene            5554..>6893
                     /locus_tag="VFU75_03705"
     CDS             5554..>6893
                     /locus_tag="VFU75_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465967.1"
                     /translation="MTTRRVLLVVAVCAVAVYLLTLWNGFVGDDGLIIVNNPLVRHWS
                     GWLPSSYGDPYWPKSFGGALYRPFPIQTYMWDWELSGGSPVWFHAINVLWNAGVAVLV
                     AVLAGAWVGGGDPAKGARAALAAGLVFAVHPVHVEAVANIVGRAELMATFFTLVAVYA
                     AIERGSWWWSALAWFFGLLCKENAAVTPALIGVAWALGIGRPRPTGGRLAGFVGSFLF
                     AGVAYSGLRYVVLGTYAPLWSPAPVFVGQSWLTVRLTAVSAFADVARLLVFPLHLRAD
                     YSPAERVAVTSAASPAFLLGVLLLAAWGFLVWGAWRRGRNVEVLGLVWIAIAFLPVAN
                     LLFPIGVLMAERTLYLPSVGLALVVGALMARAPARLAYGLAFSAVAAAAATRSVLRVP
                     VWHDNVTYAQAIRRDSPDSYQGHMAAAGILIERGRPDLALTAARRASEVFPLDPR"
BASE COUNT          995 a         2309 c         2334 g         1255 t
ORIGIN      
        1 gaggtcgacg cagccgtctc cgccctcctc tccctcatgg aacccatcat gatcgtcgtc
       61 ctcggcgtca tcgtgggcgg catggtggtc gccatgtacc tgccgatctt cgacatggtg
      121 aacgccgtcc aggggtgacg cggtagagta gggaacgcgg aactgggaac gcggaatgcg
      181 gaaccgcagt gggggttccg cattcccact tccgcgctcc gcgttcacgg ggctcccagc
      241 ctcatcccca accgtattgc attctgcgaa aataccccca tactatcctt gctatcagat
      301 tgcctcagaa tgacttaagc cgtcggaggg gatcggtggg ctggttcggc gtcaggagaa
      361 cggaggcgac ggtcccatcc ccgcagacct tgctgcgggc ggtttacgtc ggccggatct
      421 gcctggcggt ggccatctac gtcaccgccg ccgcaaaggt cgccgtcgcc gcacccctgc
      481 aggtcctggt cgtttcgttc gttctgatcg ccgccatcct cttcaccgcc tggtcctact
      541 ggcggagcca tttccgcgcg ctcccgccgt cggagacgtt cctctacctg caggcgattt
      601 tcgacgcggc gctgatcacc acggtcgtgc acatgacggg gggcgcggat tcggacttcg
      661 cgaccctgta cgtccccttg atcgccgtga tcgcggtcct catgccaccg cagtccacgg
      721 ccctggtcac cgccctcgtc gggctcgtct acttcgccga catcttcttc ggccaccgcg
      781 gcgtcatcgg acccggactc ttcatccagc tcggtgtctt cgcggccgtc gccgcggtga
      841 cggcgtactt cgcgtcgcgc gtgaaggtga tgggagccga gcgggaagcg ctggcgggcg
      901 agctccagca ggtccgcctc gaagccgacg acgtcctgcg gaacctccgg accggcgtcg
      961 tgacggtcga ctcttccggc aagctcctct actccaaccc cgcggccgag gcgctcctcg
     1021 gcttcagcgc ggacgagtgg cgggacaagc ccatcatgcc cgagctggcg cgcctggcgc
     1081 ctgagttctg ggcggcgatc atcgccaccg cccgccgcgg ggtgcggctg atgcgcgtgg
     1141 aagcgagtgt ccggcgcggg gagcaggtct ttccgatcgg tctcaccacg acgaccatcg
     1201 aaggccccaa gggggagcag ccccgggtca cggccatctt cgcggacatt tccgactcca
     1261 agcgtctcca ggagctgcac cttcgtgccg agcggctcga ggcggtggcg gagctgtcga
     1321 gctcgctggc gcacgagatc aagaacccgc tcgcgtccat ccgctcgtcc gtggagcaat
     1381 tgtcgcggtc gtcgcgcacc aatccggacg agaagttcct ggcgaatctc atcgtccggg
     1441 agagtgaccg gctgtcgcgg cttctttcgg agttcctgga cttctcgcga gtccgcgtca
     1501 ccgagtaccg ggtcctcgat ctggtggagg tggcgggcgc ggccgcccgg ttggtcgagg
     1561 agcgggaaga cttccccgaa gacgcccaga ttacgctcga gggcgccccc acccccatgg
     1621 aaggggacga ggacctggtg caccgcgtgg tgtcgaacct cgtcctcaac gccgcgcagg
     1681 cggcggggaa gggggcgcgg atcgaagtcc ggatcggccc cgcgtccccg gcagacttgc
     1741 cgtcgggggc cggcatcgag aatcccgtgc gcgtccagat cgcggataac ggccccggca
     1801 tccccgatgc cgtccgcgag cgtctcttcg agcccttcgt cacggggcgg gtggggggca
     1861 ccggtctggg cctcgccatc gtgcagcgcg ccgtcgaagc tcaccgcggt ctggtcctgg
     1921 tcgattcgac ccccgggggc ggtaccacct tcacgatcta cttccccgcc gcccggcaaa
     1981 gagaggaagc cgcatgaccg cccccgtccg gtcgtctcag ccctcgattc tggtcgtcga
     2041 tgacgaatcg gggatcctgc agaccctcca gatcctcctg aagaacgaag ggttcgacgt
     2101 caccacggcg caggggggca aggccggact cgaggcgctc aaggcgcatg cgcccgacat
     2161 cgtgctcacc gacgtccgca tgccgcaggt caccggcatc gacatcctgg ctgccgtgcg
     2221 gcaacaggac ccggaaacgc ccgtcatcct catgacggcg caggcctccc tccagacggc
     2281 gattcaggcg gtcaacgagg gtgcgttcta ctacatccag aagccgttct cgaacgacga
     2341 catggtggcg atctgccgcc gcgccgccga ctaccggaac ctccgggccg agaacaagca
     2401 gctgaaacag gagatcagaa ggcgggaacg gtcggggctg gtgaagcccc tgggcaaatc
     2461 gcgccccttc agcgacgtca tgcgtctggc cgagcaggtc gcgcccacgg aaagcaccgt
     2521 gctgatccag ggcgagtcgg gtacgggcaa agaggtcatc gcgcgctaca tccacgagct
     2581 ttccgcccga agcgaggggc ccttcctctc gctcaactgc ggagccctcc ccgagagcct
     2641 cctcgagtcc gagctgttcg ggcatgtgaa ggggtcgttc accggcgccg tgcgcgacaa
     2701 gcagggcctg ttcgcggccg cgcgcggggg gaccttcttc ctcgacgaga tcggggagat
     2761 gtcgcccgcc acccaggtga agctcctgcg cgtgctgcag gagcgtgaag cgatcccggt
     2821 gggcggcacc gaggcggttc cgctggacgt gcgggtggtg gcggcgacca accgcgacct
     2881 tgaggaggag atcaagcggg gacggttccg gaccgacctc ttttaccggc tgaacgtcat
     2941 cgccatccat ctgcctccgc tgcgcgatcg gcgcgacgac atcccgatct tcgtggacgc
     3001 cttcctcaag cggatcgcca aggagcacgg ggaggagccg aagtcgctcg cccccgatgc
     3061 actcgaagtc atcatggcgt acgactggcc cgggaacgtg cgcgagctgg agaatgccct
     3121 ggagcgggcg gtcgttctgg tgaaggccaa cctgattccg ctcagcgcca ttccgacgaa
     3181 ggtcaccgag cgcaaggccg agccgctggt gacggaagag gtgcgcacca acccgaccct
     3241 ggacgtcatc gagcaggcgt acatcacctg ggtcctgaac gccgagggtg gcaacaagac
     3301 gcgggccgcc gaagtcctgg ggatcgaccc gtcgacgctc taccggaaac tctcgaagta
     3361 cgaaggcggg aaggagccgg cgtgaagctt tcgatcatcg tgccggcgta caacgaagag
     3421 cagacggtcg aaacggtgat gaagaagctg cgggagctga aagagcccgt ggagatcatc
     3481 gccgtaaacg actgctcgac ggacgggacg ggcgcggtgc tggaccggct gaagaaggtg
     3541 ggcctggtcg accaggtgat ccatcatccc cagaaccgcg gcaaaggcgc cgcgcttcgc
     3601 gcggggttcg cggcggccac cggagaggtc gtggtggtac aggacgcgga cctggaatac
     3661 gaccccgcgg atctgcccgc cctcctcgtg cccatcaagc ggggcgtggc cgatgccgtc
     3721 ttcgggtcac gcttccaggg cggcccccac cgggtcctct acttctggca ctccgtcggc
     3781 aactcggttc tgacgctcct ctccaacatg ttcacgaatc tgaacctcac ggacatggag
     3841 acctgctaca aggtcatccg ggccgacctg atcaagaaac tgcctctcac ctccgaccgg
     3901 ttcgggttcg aggtcgagat taccgcgcgg ctggcacagg ccggggcgcg catctgggag
     3961 ctcccgatct cctactcggg ccggacgtac gccgaaggga agaagatcac gtggaaggat
     4021 ggggtggcgg cattcttcca catcgtgcgc tacaacatgt ttccaccccc gggagagtgg
     4081 cggacgccct gacccctcga caagccgggt ggatcgccgg cgccgtcgcg ctcgcggcgg
     4141 gactgttcac gctcaatcac tatctcaccg gtgtctttta cgacgacggg ctctacgccg
     4201 ggctcgcctg ggcgatggcg cacggtcacg ggttcgtgca tcccaacttg ccgggcacgc
     4261 cggcggccgt gcatttccca cccctctttc cggtcgtgtt gatcccgttc ttcgccacgc
     4321 tatcgatctc cgctgccgcg ttcgcgggga agctgctcaa cgtggtgctg ttcgccgccg
     4381 gcagcgcgct cgtcgcgtcc tacgcggtcc gtaccaagct gctcggcgag gcgcccccgt
     4441 gggtgtccgt tgccctcgtc gcggtcgccg cgatcgccat ccccgtcctc acgcttctcg
     4501 gcgccctgat gtccgagccg ctgttcgcgc ttctgctcgt cggggccgtg atgctggtgg
     4561 acgattcacc gctgtcggcg ggggtgtgcg tcgcggccgc cctgctcacg aggtcgatcg
     4621 gggtcgccgc gggggcgggg attctgctgt acgcctggcg ccgcggagca tcgcgcaggc
     4681 agctggtgga gtttgcggtc ccggtgttca tcgcgggcgc ggcctggggc ttctgggtgt
     4741 tgcgccacaa agccgggatc gatcccgccg tggcgagcga ctacggctcg tatttcgaaa
     4801 ccgtgaaatc ggccgggctg ggcgccttgg ggtcgagtac caccgacctc ccgcggcccc
     4861 tgtcggtgct caccctgaac tgggtgccga gccgggtggt ctattacgcg ctgggcatcc
     4921 ccgcgctcct ggtgggcctc tacggactgg ccctgttctg cgtgcgatcg agtatcgggt
     4981 ggactctggt cttctacctc gccatcctcg gcatctggcc gttccccccg gaccggtttc
     5041 tctgggccgt acttccctgg ctgggcctgg cgtggggagc gggggccttc gggctctggc
     5101 gccggattca gaaatggccg cgagtcgcgc tggtgctgct cgcggtggcc ctgggagggg
     5161 gatacgcccg ctacgaaatc cgcgggctct cccaccgctg gtgggacctc tcggcgcgga
     5221 gcatttcgga caacttcgcg gagctggttc ctgcgatcga ctctctcccg gcggcggcgg
     5281 tcctggcgac cgacaacgag cccctcgtct acctcgcgac cggccggcaa tcggtcccgt
     5341 tctatctctt catgtatcag ggaaggcagg tgctcgagcc gacgccgggc cttcagcggg
     5401 cctacctcga gcgttacggc gtgacgcatg tcctgctgtc cgggaccggc acggcgtccg
     5461 agggtgaatt gcgccggctg atcgccgcct atccgaccct cctgacccca gtgcggatgt
     5521 ggcccggcgg ccggaccctc tacgaggtgc gccgtgacca ctaggcgcgt gctcctcgtg
     5581 gtcgcggtct gcgcggttgc cgtttacttg ctcacgctgt ggaacggctt cgtcggggac
     5641 gacgggctca tcatcgtcaa caaccccctc gtccgtcact ggagtgggtg gctgccgtcg
     5701 tcgtatggag atccctactg gccgaagagt ttcggtggag cactctaccg cccgttcccg
     5761 atccaaacct acatgtggga ctgggagctg agcggcgggt cgcctgtctg gtttcacgcg
     5821 atcaacgttc tctggaatgc cggcgtcgcg gtgctcgtgg cggtcctggc gggagcgtgg
     5881 gtagggggcg gtgatcccgc caagggcgcg cgcgccgcct tggcggccgg gcttgtcttt
     5941 gcggtccatc cggtccacgt cgaagcggtg gccaacatcg tcgggcgtgc cgagttgatg
     6001 gccacttttt tcacgctcgt cgcggtgtat gccgcgatcg aacgcgggtc gtggtggtgg
     6061 tccgcactcg cctggttctt cggtctcctg tgcaaagaga acgcggcggt gacgccggcc
     6121 ctgatcggtg tcgcttgggc cttgggaatc gggaggccgc gtcctactgg cggacgcttg
     6181 gctgggttcg tcggctcgtt tctctttgcc ggcgtggcct attcaggtct gcggtatgtc
     6241 gtcctcggca cgtacgcgcc cctgtggtca ccggcaccgg tctttgtcgg gcagagctgg
     6301 ctgacggttc ggctcaccgc agtttccgca ttcgcggatg tggcgcgcct gctggtgttc
     6361 ccgctgcacc tgcgagcgga ctactctccc gccgagcgag tcgccgtgac gagtgccgcg
     6421 agtccggcat tcttgcttgg cgtcctgctg ctcgccgcct gggggttcct ggtgtggggc
     6481 gcgtggcgtc gcggccgaaa cgtggaagtg ctgggtctgg tctggatcgc gatcgcattc
     6541 ttgccggtgg cgaatctgtt gttcccgatc ggggtgctca tggcggagcg gacgctctat
     6601 ctcccatctg tcggcctggc gctcgtggtc ggggccctga tggcccgagc gccggcgcgg
     6661 cttgcctacg gcctggcctt cagcgcggtg gccgccgccg ctgcgacgcg gagtgtcctg
     6721 agggtcccgg tatggcatga caacgtgacg tacgcccaag ctatcaggcg ggactcacct
     6781 gattcgtacc agggccacat ggcggcggcc gggatcctca tcgagcgtgg ccggcccgat
     6841 ctcgccctga cggccgcgcg ccgcgcgagc gaggttttcc cgctggatcc acg
//
LOCUS       DASUBP010000099         7047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_452528, whole genome shotgun sequence.
ACCESSION   DASUBP010000099 DASUBP010000000
VERSION     DASUBP010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7047
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_452528"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..777)
                     /locus_tag="VFU75_03710"
     CDS             complement(<1..777)
                     /locus_tag="VFU75_03710"
                     /inference="COORDINATES: protein motif:HMM:NF016724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HEU5465968.1"
                     /translation="MIHEPLAALLTPGPEGPPPAVASRAARALLDLPPVSPATAVAPA
                     WLAVHQRPAAIRLAAIVSRYGGAVLADAPGLGKSYVAMAVALMRDEPFTLVVPAVLAH
                     QWRQLCHRFDVDPEIQTHERLGRRPFIGQVPSPRSSFRDFMSRRQLRCPLIIVDEAHH
                     YRNPATRRYRALAELAIGARILLVTATPVHNRIGDLVHLLRLFMRDDALTGLGVPSLK
                     RAAAGESWTALPAALARLVVARSRSRAPLLELPERARGMAI"
     gene            complement(767..3784)
                     /locus_tag="VFU75_03715"
     CDS             complement(767..3784)
                     /locus_tag="VFU75_03715"
                     /inference="COORDINATES: protein motif:HMM:NF014442.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HEU5465969.1"
                     /translation="MLRELLARTEQISDLGALFSALGYTPVWEQVPPASWLGERAESG
                     VKTAALVARFGAFRVFGLETREPELWARRAASRLAERAERGLIAALDARAGRLVLSAW
                     RATGKESGRLGIKAMTVPLTHPSAATIATIERLSVTADESALGLSLKIGDALASEGVT
                     PRFFRAFRDLVDRFTARLDPSIRAPDRRHLALTALTRVLFLYFVQAKGWLDGDPRYLP
                     KLLDEALTRRRHFHRSALHPLCFGALNRPYGERGAGARELGAVPFLNGGLFEPTPLER
                     EVGPALWPNPDWRDAFDDLFERFHFSVREDGSADLIAPDMLGRVFEGIMDPQDRRSSG
                     SYYTPPGLVRALVRAALETALVERRGVRPDLAARWAHEGTAPPEAPSLRGLTVVDPAV
                     GSGAFLLGALDELVRMRAAAGESRGSALSRDIISGSLFGVDLDPTAVRLTELRLWLAL
                     VADDATTVPEQVAPLPNLDGRVRQGDALLDPLALARALTGALGLGDARGEIAHLANAR
                     RTVFASPGVTKREPLRALQQAEVALARRLFEQALAGVERKISETVAAGRARDLFGRTA
                     GLPDLAREELRRLRAARWELRSSLRRLARDEGAPFFAFDTHFADVLAAGGFDCVVGNP
                     PWVRAERLPRRMRDALSTRYRCWHAGGERGFAHQPDLSVAFVERALELTATGGVIGLL
                     VPAKLATAGYAEPLRRQVANEARIARLAPADDAAAAFGAAVYPMALVAARRPPRVAER
                     VALGLDPRERTSTTQGALQQTGPWILQPHADRVARRLRDELPTVGERWAPILGVKTGA
                     DDLFLTREPCDESRPAVRGRDCGRWNVEPRLHLLWTHEPDGRVRGRLNAAMTDRLEPV
                     ADRLRQRADYRGGPPWQLFRLGLAIAPNRVIWPDLARRLSASVPEPVMAPLNTVYGIV
                     TRTVDEAHALAALFNSRWYTALAALRADPARGGFRRFNARVVRILPLPPASHVIWSAL
                     AACGRTHEVDDEAVADCLGLDARDRRALERNDP"
     gene            complement(3965..4750)
                     /locus_tag="VFU75_03720"
     CDS             complement(3965..4750)
                     /locus_tag="VFU75_03720"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEU5465970.1"
                     /translation="MSNRFVVAGLLALAIPAALPAQSKDFHWSGKLAAGQRLEIKGVN
                     GAIHATGTSSGTADVSARKHSENGRSDPDDVKIVVVNTPEAVTICAVYPTPDDADRDN
                     TCEQGSRWHSNTRDNDVVVDFTIQVPTGTEFEGQTVNGDVEAMSLTGNTDVRTVNGSI
                     DVSTSGHAEANTVNGSIRASMGKTDFEEASFRTVNGGITLTMPADLNTEVRAETVNGD
                     LDSDWPVTIVGKWGPRRMHGTIGKGGRSLTLATVNGDIRLRKS"
     gene            complement(4743..5741)
                     /locus_tag="VFU75_03725"
     CDS             complement(4743..5741)
                     /locus_tag="VFU75_03725"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEU5465971.1"
                     /translation="MLPMLLAVMLQTPAPSPNPAPRVRHVRPPKVVVNVDGGDDDRYQ
                     ANVDTTVAVTRGARLDLNNFGGDITVKVWDKNAVRISADNSDRERVDIDVDQQVVTIR
                     TSSRFGAPHMVSFDLTVPAWMALDLEGTYTDITATGIAGDITATTVQGDVDVTGGSGH
                     ISLNSTSGSVKLAKAQGRIDVVTVNDGITVTDTKGDLSAETTNGDIELRGIDADNVSA
                     TTINGDVTYDGTIKDAGHYSFSTNNGDVSVSVPQTANVAVTASTFNGDFDPCFPVTMT
                     GSHKHRFSFTLGTGSAKLEMESFGGDLKICRPGEVGKRHDTHTSKDKDKDRDDDNE"
     gene            complement(5757..6425)
                     /locus_tag="VFU75_03730"
     CDS             complement(5757..6425)
                     /locus_tag="VFU75_03730"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEU5465972.1"
                     /translation="MDRWTEKLSEYLDETLPAGERAALEAHLTTCAECRGTLDELRRV
                     VDRARGLDDRQPSKDLWSGIATRIGSKAVVSIDRARVPARAARRLSFSVPQALAAGIA
                     LMIVSSGAVWLSLRGNGSIQPPADTSRSIDMPAAWHQVDSSVDPVVANLRSTLQQNRA
                     QLNDSTVRILEKNLAIIDSAIAEARRALDADPNNNYLNHHLADVVRRRADLLRRVSTL
                     GART"
     gene            complement(6427..>7047)
                     /locus_tag="VFU75_03735"
     CDS             complement(6427..>7047)
                     /locus_tag="VFU75_03735"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU5465973.1"
                     /translation="RSHRCNRRTARTIQVRVPNTTPWIESLREPTLVTADAEIADATL
                     AASGDATAFERLYRAHVARIHSLARRMIDADEADDVTQDIFVRAWEKLSTFRAESAFG
                     TWLHRLAVNVILARRKTVGISRQRHDPREDALDAVGARPVTPELGVDFEGAMAALPEK
                     AKQVFVLHDVEGYTHEEIATMLDIVPGTSKSQLHHARMALRRHLGA"
BASE COUNT         1123 a         2365 c         2431 g         1128 t
ORIGIN      
        1 aatcgccatt ccccgggcgc gctccgggag ttcgagcaac ggtgcgcggg agcgagaccg
       61 cgccacgacg aggcgagcga gggcggcggg gagcgccgtc caggactcgc cagcggccgc
      121 ccgcttgagc gaggggactc ccagtccggt gagcgcatca tcccgcatga agagccgcaa
      181 caggtggacc agatcgccga tgcggttgtg gacgggtgtt gcggtgacaa ggagaatccg
      241 ggcaccgatc gcgagctcag cgagtgcccg atagcggcgc gtcgccgggt ttcgatagtg
      301 gtgggcctcg tcaacgatga tcaacgggca tcgcaattgc cggcgcgaca tgaagtccct
      361 aaaggacgag cgcgggctcg gcacctgccc gatgaacggg cggcggccta gccgttcatg
      421 ggtctggatc tcggggtcca cgtcaaagcg gtggcatagc tgacgccatt gatgagcgag
      481 caccgcgggc accacgagcg tgaacggctc atcacgcatc aacgcgaccg ccatggcgac
      541 atacgacttg cccagtcccg gggcgtccgc gagcaccgcg cctccgtagc gcgaaacgat
      601 cgcggctagc ctgattgccg ccggtcgctg gtggaccgcg agccatgccg gcgcgacggc
      661 cgtcgccggc gataccggcg ggaggtcgag cagagcgcgc gccgcccggg acgcgaccgc
      721 cgggggcggg ccttcgggtc cgggggtaag gagggcggcg agaggctcat ggatcattcc
      781 gctcgagcgc ccgccgatct cgtgcgtcca atccgaggca gtcggcgact gcctcatcgt
      841 ccacctcgtg cgtcctgcca cacgccgcca gcgctgacca aatcacgtgg ctggcaggag
      901 gcaggggcag gatgcgaacc acccgcgcgt tgaaacgccg gaacccgcca cgcgccggat
      961 cggcgcggag cgcggcgagg gcagtgtacc aacgggagtt gaagagcgcc gccagcgcat
     1021 gtgcctcgtc gaccgttcgc gtgacgatcc catagaccgt gttgagggga gccatcacgg
     1081 gctcaggcac cgaagcggac agccggcggg cgaggtcggg ccagatcacc cgattcggcg
     1141 cgatggcgag ccccaaccgg aagagctgcc agggtggtcc cccacggtag tcggcgcgct
     1201 gtctcaatcg atcggccacc ggctccagcc ggtccgtcat cgccgcattg agccggccgc
     1261 gcacccggcc atcgggttca tgagtccaca gcagatggag acgcggctca acattccacc
     1321 gcccgcagtc gcgcccccgg accgccgggc gagactcatc gcatggctcg cgggtcagga
     1381 acagatcgtc agcaccggtc ttgacgccga ggatcggagc ccagcgttcg ccgacagtcg
     1441 gcagctcgtc ccggagccga cgagccacgc gatcggcatg aggctggagg atccaggggc
     1501 ccgtctgctg gagcgcgccc tgggtggtgg aggttcgctc gcgtgggtcg agaccgagcg
     1561 cgacccgctc ggcgacacgc ggcggccgtc gcgcggcgac cagcgccatg gggtacacgg
     1621 ccgcaccgaa ggccgccgcg gcgtcatccg cgggagccag tcgcgcgatc cgcgcttcgt
     1681 tcgcgacctg ccgccgcaac ggctccgcgt acccagccgt cgcgagcttt gcgggaacga
     1741 gcagaccaat cactccgccg gtggccgtga gctcgagtgc gcgctcgacg aacgccaccg
     1801 acaggtcagg ctgatgtgcg aacccacgtt ctccacccgc gtgccagcag cggtatcgcg
     1861 tggacagcgc atccctcatg cgccgcggca gtcgttcggc gcgtacccag ggaggatttc
     1921 cgacgacgca atcgaaccca ccggcggcaa ggacgtcggc gaagtgggtg tcgaaagcga
     1981 agaacggagc accttcgtcg cgagccagac gccggaggga ggagcggagc tcccaccggg
     2041 ccgcccgaag ccggcgcagc tcctcgcggg ccaggtccgg gagcccggcg gtgcggccga
     2101 agaggtcccg cgcccgtccc gccgcgacgg tctcggagat ctttcgctcg actcccgcga
     2161 gcgcctgctc gaagagccgc cgggcgagcg cgacctccgc ctgctggagt gctcggagtg
     2221 gttcccgttt ggtcacaccc ggagatgcaa agacggtccg tcgagcgttc gcgagatggg
     2281 cgatctcgcc ccgcgcgtct cccaggccca gcgcacccgt gagggcgcga gccagcgcca
     2341 gcgggtcgag aagggcatcc ccctgacgta ccctgccgtc gaggttcggg aggggagcca
     2401 cttgctcggg gaccgtggtg gcgtcgtcgg ccaccagggc gagccagagt ctcagctcgg
     2461 tcagccggac tgccgtggga tcgagatcga cgccgaagag cgatcccgag atgatgtcgc
     2521 gggagagcgc ggaaccgcga ctctctcccg cggccgcgcg catccgcacc agctcgtcga
     2581 gcgcgcccag cagaaacgct cccgagccca ccgccgggtc gaccacggtg aggccgcgca
     2641 gactcggcgc ctccggcggg gcagtgcctt cgtgcgccca gcgcgccgcg agatcgggcc
     2701 gcactccgcg gcgctcgacc agtgccgtct cgagtgctgc ccgcacaagg gcccggacca
     2761 gtcccggcgg cgtgtagtag ctgccgctcg agcggcggtc ctggggatcc atgatcccct
     2821 cgaacacccg gcccagcatg tcaggggcga tcaggtcggc gctcccgtcc tcgcggaccg
     2881 agaagtggaa tcgctcgaac aggtcgtcga acgcatcccg ccagtcaggg ttgggccaca
     2941 gggcgggccc gacctctcgc tcgaggggcg tgggctcgaa gagcccgcca ttcaggaagg
     3001 gcacggcgcc gagctcacgg gcaccagctc cccgttcgcc atagggacgg ttcaaggcgc
     3061 cgaagcagag cgggtgcagg gcggaccgat ggaaatgacg ccgccgcgtg agggcctcgt
     3121 cgaggagctt ggggaggtac cgcgggtccc cgtcgagcca gcctttggcc tgcacgaaat
     3181 agaggaacag gacgcgggtg agcgccgtga gggcgaggtg gcggcggtcg ggagcccgga
     3241 tggagggatc cagccgggcg gtgaaccggt cgacgaggtc gcggaaggca cggaagaacc
     3301 tcggcgtgac cccctcgctc gcgagggcat cgccgatctt gagcgagagg ccaagcgccg
     3361 actcgtcagc ggtgacggac agacgctcga tcgtcgcgat cgtggccgcg gacgggtgag
     3421 tgagggggac cgtcatcgcc ttgatgccga gccgccctga ttccttgccg gtcgcgcgcc
     3481 acgccgagag gacgagtctg cccgctcggg cgtcgagcgc cgcgatcagg ccccgctcag
     3541 cccgctcggc gagtctcgag gcggcgcggc gggcccacag ctcgggttcc cgggtctcga
     3601 ggccgaacac ccggaacgcg ccgaaccgcg ccaccagcgc cgcggtcttt acccccgact
     3661 cggcgcgctc tcccaaccag gaggccgggg gcacctgctc ccacacgggt gtgtagccga
     3721 gcgcgctgaa caacgcccca agatctgaga tctgctcggt ccgggcgaga agctcccgaa
     3781 gcactccggc ccctggttga agaggccctc aacatcagtg tgcttggaga gatcagcggt
     3841 atcgaattac tgcggtgagg aacgaaaaaa cggggaggag agaggaggga cgggtcgcag
     3901 tccgggaagt ggaggctcca cttcactcga cttcgactcc tcccccgtcc ctcctcccaa
     3961 agactcagct ctttctgagt ctgatatccc cattgaccgt cgccagcgtc agcgaccgtc
     4021 cacccttgcc gatcgtcccg tgcatccgac gcgggcccca cttgcccacg atcgtcaccg
     4081 gccagtcgga atccagatcg ccattcaccg tctcggccct cacttcggtg ttcaggtcgg
     4141 ccggcatggt cagcgtgatg ccgccattca cggtgcggaa tgacgcctct tcgaagtcgg
     4201 tcttgcccat cgacgcccgg atcgatccgt tcaccgtgtt cgcttccgcg tgcccggagg
     4261 tcgacacgtc gatgctgccg ttcaccgtgc gcacgtcggt gttgccggtc agggacatgg
     4321 cctcgacgtc gccgttcacc gtctggccct cgaactcggt gcccgtcggg acctggatcg
     4381 tgaaatcgac gaccacgtcg ttgtcgcggg tgttcgaatg ccagcggctg ccctgctcgc
     4441 aggtattgtc gcggtcggcg tcatccggag tcgggtagac cgcgcagatg gtcaccgctt
     4501 ccggtgtgtt caccaccacg atcttcacgt catccggatc ggaacgtccg ttctcggagt
     4561 gcttccgggc gctgacgtcc gcggtgccgc tgctggtgcc ggtggcgtgg atcgcgccgt
     4621 tcacgccctt gatctcgagg cgctggccgg cggcgagctt gcccgaccag tggaagtcct
     4681 tgctttgagc gggcaatgcc gccgggatcg ccagggccag caggcccgcc accacgaagc
     4741 gattactcat tgtcgtcgtc cctgtccttg tctttgtcct tcgacgtgtg cgtgtcgtgg
     4801 cgttttccca cttcacccgg gcggcagatc ttcagatctc cgccgaatga ctccatttcg
     4861 agctttgccg agcccgtgcc caacgtgaag ctgaaccggt gcttgtgact gccggtcatc
     4921 gtgaccggga agcagggatc gaaatccccg ttgaaggtcg acgccgtcac ggccacgttc
     4981 gcggtctgcg gcacggacac gctcacgtcg ccgttgttcg tcgagaagct gtagtgcccg
     5041 gcgtccttga tggtcccgtc atacgtgacg tccccattga tggtggtcgc cgacacgttg
     5101 tccgcgtcga tgcccctgag ctcgatgtcg ccattcgtcg tctcagcgga caggtcgccc
     5161 ttcgtgtccg tcacggtgat gccgtcattc accgtcacca cgtcaattcg gccctgggcc
     5221 ttggccagtt tcaccgagcc cgaggtggag ttgagggaga tgtgtcccga tccgccggtc
     5281 acgtcgacgt cgccctgcac cgtggtggcc gtgatatcgc cggcgattcc cgtggcggtg
     5341 atgtccgtgt aggtgccttc gagatcgagc gccatccacg ccggcaccgt caggtcgaac
     5401 gacaccatgt gcggggcgcc gaagcggctc gacgtacgga tggtcaccac ctgctgatcc
     5461 acgtcgatgt ccacccgctc gcggtccgag ttgtcggccg agatccggac ggcgttcttg
     5521 tcccacacct tcacggtgat gtcgccgccg aagttgttga ggtcgagccg ggcaccgcgc
     5581 gtgacggcga ccgtggtgtc gacgtttgcc tggtaccggt cgtcgtcacc cccgtcgacg
     5641 ttcacgacca ctttcggggg ccggacatgg cggactcggg gcgcgggatt ggggctcggc
     5701 gccggagtct ggagcatgac ggccaggagc atgggaagca tgggtttcag gtcctctcag
     5761 gttcgcgcgc cgagggtgct gactcggcga agcaggtccg ccctgcgccg gacgacgtcg
     5821 gcgaggtggt ggttcaggta gttgttgttc gggtcggcat cgagcgcgcg ccgcgcctcg
     5881 gcgatcgcgg agtcgatgat ggccaggttc ttctcgagaa tccggaccgt actgtcgttg
     5941 agctgggccc ggttctgctg caaggtggat ctcaggttgg ccacgaccgg atcgacggac
     6001 gagtccacct ggtgccaggc ggccggcatg tcgatggagc ggctcgtatc cgccggtggc
     6061 tgaattgacc cgttacctcg caatgacagc cacaccgcgc ctgacgacac gatcatcagc
     6121 gcgatgccgg ccgccaaagc ctgaggcacg gagaacgaga gacgacgggc ggcccgcgcc
     6181 ggaacgcgcg cgcggtcaat cgagacgacg gccttcgatc cgatccgggt cgcgatccct
     6241 gaccagaggt ccttcgacgg ctgacggtca tcgagcccgc gcgcgcggtc gaccacgcgg
     6301 cggagttcat ccagggtccc gcggcactcg gcgcacgtgg tgaggtgggc ttccaatgcc
     6361 gcacgctcgc ccgcggggag tgtctcgtcg agatactcag agagtttttc agtccagcgg
     6421 tccatactag gctcctaggt ggcgtcgcaa cgccattctt gcgtgatgca attgagactt
     6481 cgacgtaccc ggcacgatgt cgagcatcgt cgcgatttcc tcgtgggtgt agccttccac
     6541 gtcgtgcagc acgaacacct gcttcgcctt ctcgggcagt gcggccatcg cgccttcgaa
     6601 gtccactccc aattcgggcg tcaccggtct cgctcccacc gcatcgagcg cgtcttcgcg
     6661 tgggtcgtga cgctggcgcg agatcccgac cgtctttcgc cgggcgagga tcacgttcac
     6721 cgccagccgg tgcagccagg tgccgaacgc tgactcagcg cgaaacgtcg agagcttttc
     6781 ccacgcgcgg acgaaaatgt cctgcgtcac gtcgtcggct tcgtcggcat cgatcatccg
     6841 ccgggccagc gagtgaatgc gggccacgtg ggcgcgatag agccgctcga aggccgtcgc
     6901 gtcaccgctc gcggccagag ttgcgtcagc tatttcggcg tcggcggtga ccagtgtcgg
     6961 ctctcgtagg ctttcgatcc agggagtcgt attcggcaca cggacttgga tagtccgggc
     7021 ggtgcgacgg ttgcaacgat gtgaccg
//
LOCUS       DASUBP010000100         2704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1269739, whole genome shotgun sequence.
ACCESSION   DASUBP010000100 DASUBP010000000
VERSION     DASUBP010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2704
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1269739"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..508
                     /locus_tag="VFU75_03740"
     CDS             <1..508
                     /locus_tag="VFU75_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HEU5465974.1"
                     /translation="LLGEPNAGKSTLLSVISAARPKIADYPFTTLEPNLGVVGLSDNR
                     SFVVADVPGIIEGAHAGKGLGLKFLQHVERTRLIAVLVPADSPDPQATYELLRGEALR
                     YSEELAKKPHLVVITKTDLLPSGDPLPVIHTHDGARVCAISAVSRAGVPELLETLWQT
                     LRAVPATT"
     gene            472..1584
                     /gene="dprA"
                     /locus_tag="VFU75_03745"
     CDS             472..1584
                     /gene="dprA"
                     /locus_tag="VFU75_03745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HEU5465975.1"
                     /translation="MADLARRSRDDVNGLAATLALSQIPGIGPVRLRQLIAAYGSAES
                     ALAAQNGRLDAGLAILDRLKELNARVLLADDPEFPPMLAEMSDPPALLYVWGDLAQAR
                     REAVAFVGSRNCTPYGLEAARILARDVGATGKVTIVSGMARGIDTAAHQAALDVGAGS
                     VGVLGNGFGIVYPPSNERLYERMVAHGCLITELPPDQPPYQGAFPRRNRLISGLARVT
                     VVVEAAVKSGALITVDAALNQGRTVLAVPGPITSLTSEGCNKLIQQGATPALTAGDIL
                     EELGLPRGDAAGAGDGIRDTGNGKREVREVERTPPSDLDPVQRSLWDAMGPELRHVDA
                     LVAAGGGDAGKVLVALTDLEMRGIVRQEPGMRFGLR"
     gene            1731..2132
                     /locus_tag="VFU75_03750"
     CDS             1731..2132
                     /locus_tag="VFU75_03750"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEU5465976.1"
                     /translation="MFGGTIHKAGGLYAHITWHTWRRGRVIKRVDVPVIIAAINEAAS
                     RTAARVLSIAVLRDHVHVVIRQAAHSPLAGFIRHAKSESSRRVNIQRAGEQVLRWGRG
                     YYAGSISWGHLAAARNYVGGQFKRHPDLIPE"
     gene            2339..>2704
                     /locus_tag="VFU75_03755"
     CDS             2339..>2704
                     /locus_tag="VFU75_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465977.1"
                     /translation="MHLYLHIPFCQRRCSYCDFSIAVRKTIPAREYVAAIRNEIAQVA
                     AESGKREAGSGTSEDRSPTLPSSRFPPPDVPTIYLGGGTPSLLPPEAIGELIASLHAA
                     FPARISPTRFASLSQFEVTL"
BASE COUNT          423 a          939 c          910 g          432 t
ORIGIN      
        1 cctcctcggc gagccgaacg ccgggaagag cacgctcctc tccgtcatct cggcggcgcg
       61 ccccaagatc gccgactatc cattcaccac cctcgagccc aacctcggcg tcgtcggact
      121 ctcggacaac cggtccttcg tggtcgccga cgtgcccggc atcatcgaag gcgcgcacgc
      181 ggggaagggg ctgggcctga agttcctcca gcacgtggag cgcacccgcc tcatcgccgt
      241 cctcgtgccc gccgacagtc ccgatccaca ggccacctac gagctgcttc ggggcgaagc
      301 gctccgctac tcggaagagc tggccaagaa gccgcacctc gtcgtgatca cgaagaccga
      361 tcttctgccg tcaggggatc cgctgcccgt catccacacc cacgatggcg cccgcgtctg
      421 cgccatctcg gccgtgagtc gggcgggggt accggagctg ttggagaccc tgtggcagac
      481 cttgcgcgcc gttcccgcga cgacgtaaac ggcttagccg ccacactcgc gctctcccaa
      541 atccccggca tcggtcccgt ccggctgcgg cagctcatcg ccgcatacgg aagcgccgag
      601 tcggccctcg cggcgcagaa cgggcggctc gacgccggcc tcgccattct cgaccgtctg
      661 aaagagctga acgcccgggt gctcctcgcg gacgatccgg aattccctcc catgctggcc
      721 gagatgtccg atccgccggc cttgctgtac gtgtggggag acctcgcgca ggcgcgccgc
      781 gaggcggtcg ccttcgtggg atcgcgaaat tgcaccccct acggactcga ggccgcacgg
      841 atcctcgcgc gcgacgtcgg cgcgaccggc aaggtcacca tcgtgagcgg catggcgcgg
      901 gggatcgata ccgcggccca ccaggcggcc ctcgacgtgg gcgccggctc cgtcggcgtc
      961 ctcggcaacg gcttcggcat cgtctatccg ccttcgaatg agcggctgta cgaacggatg
     1021 gtcgcgcacg ggtgcctgat caccgagctc ccacccgacc agcctccgta ccagggcgcg
     1081 ttcccgcggc ggaatcgtct gatctccggt ctcgcccgag tcaccgtggt ggtcgaggcc
     1141 gccgtgaagt cgggggcact gatcactgtc gacgcggcgc tcaaccaggg acgcacggtg
     1201 ctcgccgtgc cgggcccgat caccagcctc acgtcggagg ggtgcaacaa gctcatccag
     1261 cagggtgcca ctcccgcgct gaccgcgggg gacattctcg aggagctcgg gttgccgcgg
     1321 ggagatgcgg cgggggcggg agacgggata cgtgacacgg gaaacgggaa acgtgaggtg
     1381 agggaggtcg agcggacacc gccctcggac ctcgatcccg tccagcggtc cctctgggat
     1441 gcgatggggc ctgagctccg gcacgtcgac gcgcttgtcg cggcaggggg aggggacgcg
     1501 gggaaggtgc tcgtcgcgct cacggatctc gagatgcggg ggatcgtgag gcaggagccg
     1561 gggatgcggt tcgggctgag gtgagcgcac cccttggttc gccgggccgc cggcccggcg
     1621 tcactcgacc cactcctggc attgttgtga actcccgggg catggcctag cgggaactgg
     1681 gttttcgccg ggccgccggc ccggcgtcac tcgacccaag gagaatcgcg gtgtttggtg
     1741 gcaccattca caaggccggc ggcttgtacg cccacatcac gtggcacacc tggcgtaggg
     1801 gcagggtgat caagcgggtt gacgtccccg tgatcatcgc ggcgatcaat gaagcggctt
     1861 ccaggaccgc tgctcgggtg ctgagcatcg cggtactcag ggatcacgtg cacgtcgtca
     1921 tccggcaggc ggcgcattcg cctctcgcgg ggttcattcg gcatgccaag tcggaatcgt
     1981 cgcgacgcgt gaacattcag cgggcgggtg agcaggtgct gcgttggggg aggggctatt
     2041 acgcgggatc aatttcctgg ggccacctcg cggctgcgcg gaactacgtg ggagggcagt
     2101 tcaagcggca ccccgacttg attccggaat aggtcagggt tgggtaacgc cggggcggtg
     2161 ccccggcgca actccaggat caacggaggt tcgcaggtgg aagcggtgcc ccggcgcaac
     2221 tccaggatga acggatgttc gcaggtggaa gcggtccccc ggcgccaggc cccaggggcc
     2281 aggaggagtg tgaccccgat ccctgacccc cgacccccga cccctgtact ttccccccgt
     2341 gcacctgtac ctccacatcc ccttctgcca acgacggtgc tcctactgcg acttctccat
     2401 cgcggtgcgg aagacgatac cggccagaga gtacgtcgcc gcgattcgga acgagatcgc
     2461 acaggtagct gcggagtccg ggaagcggga agcgggaagc gggacgagtg aggaccgctc
     2521 tcccacgctt ccctcttccc gcttccctcc tcccgacgtc cccaccatct acctgggcgg
     2581 cggcactccc tccctgctgc ctccggaggc cattggcgag ctgatcgcct cgctccacgc
     2641 cgcattcccc gcacgcattt cgcccacccg cttcgcgtcg ctgtcgcaat tcgaggtgac
     2701 gctc
//
LOCUS       DASUBP010000101         2425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1038234, whole genome shotgun sequence.
ACCESSION   DASUBP010000101 DASUBP010000000
VERSION     DASUBP010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2425
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038234"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1683
                     /locus_tag="VFU75_03760"
     CDS             <1..1683
                     /locus_tag="VFU75_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HEU5465978.1"
                     /translation="KTYDAIVVGSGMTGGWAAKELTEHGLETLVLEAGPMVTPADYVE
                     HVPPWEKKFRGLRDRKRQLERQPIQKECYACDEWSGKFFVDDIDNPYTQAEGKPFLWL
                     RGRHVAGRSIMWGRQVYRWSDLDFEGNLRDGIAVDWPIRYQDIEPWYDHVEEFIGISG
                     EKLGLSQLPDGKFLKPMDLNCGESMMRDVIAKHWGGERVLTIGRCAVLTENHNGRAAC
                     HYCGYCERGCITLSYFNSINATLPAAQKTGKMTLRPDSVVHSLIYDKKKNRISGVRVI
                     DGTTMHAIEFHARVVFLCASALESARLLLNSATPDLPHGLANSSGEVGHNIMDHIMGG
                     GASGRIDAPADRNTVGRRPNGIYVPRFRNLAGGPKSKFLRGYGFQGGAGRDGWWRGTD
                     LPGFGADWKKQLEGPGDWHMGFSGFGECLPNHENYLEIDKTKNDKWGVPVIRISVAYS
                     DNERQLMEDSSIAAAEMLAAAGARDISVGTEKDFSPPGKAIHEMGTCRMGRDPKTSVL
                     NANNQAWDIPNLFITDGACMTSSACQNPSITYMALTARAANFAVDAMKRNEI"
     gene            1680..>2425
                     /locus_tag="VFU75_03765"
     CDS             1680..>2425
                     /locus_tag="VFU75_03765"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEU5465979.1"
                     /translation="MTMQTGNGNRETGNVGRREFLGAAGAGLAAVSPALRYVSRFSLP
                     VSRISKVGLQLYTVRDLLRTDFEGTLKKVAAIGYKEVELTDFFGRTSSEVKALLDGLG
                     LSPVSMHFDYAKLPAGLDAMIADAHTVGLSYITFAWIDEKLRPDIDGWKRVAESLNKT
                     GEKVKAAGLQLAYHNHSYEFKPLQGQLPYDVLLANTDPKLLQGEMDLFWITDGGQDPL
                     AYFAKYPGRFTQVHVKDMGPGPGPDHTMVDV"
BASE COUNT          466 a          747 c          818 g          394 t
ORIGIN      
        1 aaaacctacg acgcgatcgt cgtcggctcg gggatgacgg gcggctgggc cgccaaggag
       61 ctcaccgagc acgggctcga aactctggtt ctcgaggccg gtccgatggt gacgcccgcc
      121 gactatgtgg agcacgtccc accctgggag aagaaattcc ggggcctgcg ggaccggaag
      181 cggcagctcg agcggcagcc gatccagaag gagtgctacg cctgcgacga atggtcgggg
      241 aagttcttcg tggacgacat cgacaatccc tacacgcagg ccgaggggaa gcccttcctc
      301 tggctgcggg ggcggcacgt ggcggggcgg tcgatcatgt gggggcgcca ggtctaccgc
      361 tggagcgacc tggacttcga aggcaacctg cgcgacggca tcgcggtcga ctggccgatc
      421 cggtaccagg acatcgagcc ctggtacgat cacgtcgagg aattcatcgg catctccggc
      481 gaaaagctcg gcctctcaca gctgccggac gggaagttcc tgaagccgat ggatctgaat
      541 tgcggggagt cgatgatgcg ggacgtgatc gccaagcact ggggtgggga gcgagtgctc
      601 accatcggcc ggtgcgcggt gctcacggag aaccataacg gacgcgcggc ctgtcactac
      661 tgcgggtatt gcgagcgcgg ctgcatcacg ctctcctact tcaactcgat caacgcgacg
      721 ctgccggcgg cgcagaagac cgggaagatg acgctgcgtc ccgacagcgt ggtccattcc
      781 ctcatttacg acaagaagaa gaaccggatt tcgggcgtcc gggtgatcga cggcaccacg
      841 atgcatgcga tcgaattcca cgcccgcgtg gtgttcctct gcgcgtcggc gctcgagtcc
      901 gcccgcctcc tgctgaactc ggcgactccg gacctgcccc acgggctcgc caattcgagc
      961 ggagaagtcg ggcacaacat catggatcac atcatggggg gcggggcgtc cgggcgcatc
     1021 gacgcccccg ccgaccggaa caccgtgggc cgccgtccca acggcatcta cgtcccgcgc
     1081 ttccgcaatc tggccggagg cccgaaatcc aagttcctcc gcgggtacgg atttcagggg
     1141 ggcgcggggc gggacgggtg gtggcggggt acggatctcc ccggcttcgg cgccgactgg
     1201 aagaagcagc tcgaggggcc cggtgactgg cacatggggt tctcggggtt cggggagtgc
     1261 ctccccaacc acgagaacta cctcgagatc gacaagacca agaacgacaa gtggggcgtt
     1321 ccggtcatca ggatcagcgt ggcgtactcg gacaatgagc gccagctgat ggaagactcg
     1381 tccattgcgg cggctgaaat gctggcggcg gcaggggcga gggacatctc ggtggggacc
     1441 gagaaggact tttccccgcc ggggaaggcg atccatgaga tgggcacctg ccgcatgggc
     1501 cgggatccca agacgtccgt cctgaatgcg aacaatcagg cgtgggacat cccgaatctg
     1561 ttcatcaccg acggcgcctg catgacttct tcggcgtgcc agaacccgtc gatcacctac
     1621 atggcgctca ctgcgcgggc cgcgaacttc gcggtggacg cgatgaagag gaacgagata
     1681 tgaccatgca aacgggaaac gggaatcgtg aaacgggaaa cgtgggaaga cgggaattcc
     1741 ttggggcggc cggagccggc ttggcagccg tgagtcccgc gctgcgctac gtttcccgtt
     1801 tctcgcttcc cgtttcccgt atctcgaaag tcggcctcca gctctacacg gttcgcgacc
     1861 tcctgaggac cgacttcgaa ggcacgttga agaaggtcgc cgcgatcggc tacaaggaag
     1921 tcgagctcac ggacttcttc ggccgcacgt cctcggaggt gaaagcgttg ctggacggcc
     1981 tgggactttc gcccgtgtcg atgcacttcg actatgcgaa gctgccggcc ggcctggatg
     2041 cgatgatcgc agacgcgcac accgtgggcc tctcttacat cacgttcgcc tggatcgacg
     2101 agaagctgcg cccggacatc gacggctgga agcgcgtggc cgagtcgctc aacaagaccg
     2161 gggagaaggt gaaggccgcg ggattgcagc tggcctatca caaccacagc tacgagttca
     2221 aaccgctcca ggggcagctg ccgtacgacg tcctgctcgc caacacggac cccaagctgc
     2281 ttcagggcga gatggacctg ttctggatca ccgatggggg gcaggacccc ctcgcctact
     2341 tcgcgaagta tcccggccgg ttcacccagg tgcacgtgaa ggacatgggc cccgggccgg
     2401 gtcccgatca caccatggtg gacgt
//
LOCUS       DASUBP010000102         2520 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_560993, whole genome shotgun sequence.
ACCESSION   DASUBP010000102 DASUBP010000000
VERSION     DASUBP010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2520)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2520)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2520
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560993"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(45..659)
                     /locus_tag="VFU75_03770"
     CDS             complement(45..659)
                     /locus_tag="VFU75_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465980.1"
                     /translation="MRRLQWLMLAAVVGCTDATTSPSAPPVTTTPTSQLHIVAQDTAA
                     PALLSDSVSFYAVAGQGRQVKMYYQGAAPGDTGETLLEFEVPGDGLLKKPDGTSFQTG
                     DSILITISVATPGRFEFTFGPTGLLFNPASPARLKIEYGHCDHDFNDDGHIDPADSTT
                     QTLLDVWRRQAGDTLWTKLGGVNLDEDNEVSVNVLHFTQYALAW"
     gene            complement(778..2052)
                     /locus_tag="VFU75_03775"
     CDS             complement(778..2052)
                     /locus_tag="VFU75_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEU5465981.1"
                     /translation="MGAELRRSTPPESPSASVGAVAVIVAAGGVEPDLPQAIPALAVP
                     PGTPVFAVGAAVSHRVAARAVAAGASDYFALPGDRDALQSALEAAIDRRRSALDRAAL
                     AHLEAQSHAFKEIVGESPALRKALDRAARVLPHADATVLITGETGTGKELLARAVHYG
                     GPRAAAPFVELNCAALPPQLLESEIFGHERGAFTDAKSSKPGLLEVAHGGTLFLDEVD
                     RLTPELQGKLLRAIELKTARRLGATTSRTFDVRIVAATNADLGNAVREGRFREDLYYR
                     LKVVTLSLPPLRERGDDVIRLADFFIEKLSGQYGLPRPVITKEVRAVILAQPWPGNVR
                     ELRNTIERVILLSPPGSLDVTELNAPGSAAPPRPGTLPFPARLAEIQRAAAHAMLKEA
                     DGNHSEAARRLGISRSRLARLLKGVDDTPDSD"
     gene            complement(2305..>2520)
                     /locus_tag="VFU75_03780"
     CDS             complement(2305..>2520)
                     /locus_tag="VFU75_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465982.1"
                     /translation="EEFSVAVLGRPLQAVQAYWRAKTYREDVTPPPELLNDQAVLDYV
                     KTHPNAIGYVTPDADLPAGVHAVVVTR"
BASE COUNT          408 a          785 c          906 g          421 t
ORIGIN      
        1 tccgcgccgg tcgtcatccc ggactcccga tcacccggcc gcgctcacca ggcgagggcg
       61 tactgggtga aatggaggac gttgacgctg acttcattgt cctcgtccag gttcacgcca
      121 cccagcttgg tccacagggt gtccccggcc tgccggcgcc agacgtccag caacgtttgt
      181 gtcgtggaat cggccggatc gatgtggcca tcatcattga agtcgtgatc acaatgaccg
      241 tactcgatct tgaggcgggc gggcgatgcg ggattgaaca gcaggccggt gggtccgaac
      301 gtgaactcga accgccccgg ggtggccacc gagatcgtga tcaggatgga gtcgcccgtc
      361 tggaaggacg tgccatcagg cttcttcagc agtccgtctc cagggacctc gaactcgagg
      421 agggtttccc cggtgtcccc gggtgcggcg ccctggtaat acatcttcac ctggcgcccc
      481 tggccggcga cggcgtagaa cgacacgctg tcgctcagca gagcgggggc agcggtgtcc
      541 tgagcgacaa tgtgcagctg ggatgtcggc gtggtggtga ccggtggcgc cgacggggac
      601 gtcgtcgcat cggtacaacc aaccacagcg gccaacatga gccactgtag ccttcgcatc
      661 atggttctcc gggtgtcagg gtgttgggca cttgatttgc aaggcctacg ccaagcgtaa
      721 gcgtttgcgc tgtcgtggtt tgggtctggg ggaatgactc ggcgcgagcc aactggctca
      781 atccgagtca ggcgtgtcgt ccacaccctt cagcaggcgg gccagccgcg aacgggagat
      841 gccgagacgg cgcgccgctt cggagtgatt gccgtccgct tccttgagca tggcgtgcgc
      901 cgcggcccgc tggatttcag ccagccgggc ggggaacggc agagtccccg gccgcggggg
      961 cgcagcgctc ccgggggcat tgagctcggt gacgtccagc gagccgggcg gcgagagcag
     1021 gatcacccgt tcgatggtgt tgcggagctc gcgcacgttg ccgggccagg gttgggccag
     1081 gatcaccgcg cggacctcct tggtgattac cggtcggggg aggccgtatt gcccggagag
     1141 cttttcgatg aagaagtcgg cgagccggat cacgtcgtcc ccccgttcgc gaagcggggg
     1201 aagggacagc gtgacgacct tcagccggta atagagatct tctctgaagc gcccctcccg
     1261 cactgcgttg cccaggtctg cattcgtggc cgcgacgatc cggacgtcga aggtacggct
     1321 ggtcgtggcg ccgagacgcc gggcggtctt cagctcgatg gcgcgcagga gcttgccctg
     1381 caactcgggg gtaaggcggt cgacctcgtc gaggaagagc gtgccaccat gcgcgacttc
     1441 caggagcccg ggtttggagc tcttggcgtc cgtgaatgcg ccccgctcat gcccgaagat
     1501 ctcgctctcc aggagctggg gagggagcgc ggcgcagttg agttcgacga agggagccgc
     1561 ggcccgcggt ccgccgtaat gcacggcgcg cgcgagcagc tccttgccgg ttcccgtttc
     1621 tccggtaatg aggaccgttg catctgcgtg gggcaggacg cgtgccgcgc gatcgagtgc
     1681 cttgcgcagc gcggggctct cgccgacgat ttccttgaag gcgtgcgact gggcttcgag
     1741 gtgggccagg gcggcgcggt cgagggcact ccggcgccga tcgatggcgg cctcgagtgc
     1801 ggattgcagc gcgtcccggt cgccgggcag cgcgaaatag tcggacgccc ccgccgccac
     1861 ggcccgcgcc gccacccgat gggagacggc cgcgcccacc gcgaacacgg gcgttccggg
     1921 tggaaccgcg agagcaggaa tggcctgagg gaggtcgggc tcgactccac cggccgcgac
     1981 gatgacggcg acggctccga ccgaggctga tggggattcc ggcggcgtcg accgccgaag
     2041 ctcggcaccc aaagcagcag cgagcgatcc ccagtggtcc gagaaggagt cgctaagatc
     2101 gacgacgagg aaccaggtgg cccggcgcgg ctcggcggat gagccggtcc ggggcgtcga
     2161 accagtggtc agagctgctc ctgttacctg gggcgcggct gatacgaacg gaaattctac
     2221 cccgaggtta cagagagtta cagggcgatc gtgaagtccc cttcacgatc gccctgttct
     2281 gctatacgcc tacgcgctca gtacttagcg ggtgaccacc accgcgtgca cgccggccgg
     2341 aaggtcggcg tcgggcgtga cgtagccgat cgcgttcgga tgcgtcttca cgtaatcgag
     2401 cacggcctgg tcgttcagga gctcaggcgg aggcgtaacg tcttcgcggt acgtcttggc
     2461 gcgccagtac gcctgaacgg cctggagcgg gcggcccagg acggcgaccg agaactcttc
//
LOCUS       DASUBP010000103         2806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1229200, whole genome shotgun sequence.
ACCESSION   DASUBP010000103 DASUBP010000000
VERSION     DASUBP010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2806
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229200"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..1844
                     /locus_tag="VFU75_03785"
     CDS             111..1844
                     /locus_tag="VFU75_03785"
                     /EC_number="1.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent dehydrogenase, methanol/ethanol
                     family"
                     /protein_id="HEU5465983.1"
                     /translation="MLPLLLAAAILTQTPDDGQWTMPGKNFAATRFSSLTSITTSNAA
                     QLRPLWSFSTGVLGGHEGQPLVVNNTMYVVTPFPDVLYAFDLTKDGYPLKWKYRPDVN
                     PSALGTACCDAINRGPVYADGKIVYNLLDGHTVAVDATTGQELWKVQVADLGQGETMP
                     MAPLVVRDLVFVGPSGGEFGIHGWLKALDLANGKVVWTAYNEGPDKDMLIDTSKFHPF
                     YDHGKDLGLTTWPGDAWQRAGIPVWGWLSYDPDLDLLYYGTGNAAPYNPEQRAGDNKW
                     ASSVLARRPRDGALVWAYQFTPHENWDYDANAEMILADLNIGGKVRHALVHFDKNGFA
                     YTIDRETGEVLVAKPFTTVTWAKSINLKTGRPVLDSAKLTGASRGNVKDICPSLEGGK
                     SPVSAGSYSPHTEYFYVATNNLCMDYQASQVSHIAGTPYIGANTPYHAGPGGYMGEFI
                     AWDAVNGKKVWGITEKYPVWSGSVTTAGDVVFYGTLDGWFKAANAWTGQVLWKFKVGS
                     GVVGCPIVFKGADGKQYVAVYAGIGGDWFLLSGDVRSDDPADVRPPADFAKDLARHTS
                     QGGLVWIFGLP"
     gene            1823..2281
                     /locus_tag="VFU75_03790"
     CDS             1823..2281
                     /locus_tag="VFU75_03790"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU5465984.1"
                     /translation="MDLRLALIGATALGAVAVACKPVTPANTGRYVPPVISYPQHVNV
                     AGIAPAAGTLTNPYEPDSASVREGGQTFTAMNCDGCHGGGATGWVGPSLVDGRWRYGG
                     EDGAVFTSIFYGRPRGMPAYGGTLSPAAIWKIVTYIRAQPVPTDVPTERW"
     gene            2275..2478
                     /locus_tag="VFU75_03795"
     CDS             2275..2478
                     /locus_tag="VFU75_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465985.1"
                     /translation="MVSEVCVPNIGPRERRKRLYAGFGYLGLAIVALAIMTATGTPRG
                     YRLLLFLPLWAAGVGVFQYLEKT"
BASE COUNT          485 a          898 c          926 g          497 t
ORIGIN      
        1 ggttgggggg ccctgcttgg tcgcgtcgcc gctacggcca cgaagatggc cctgggcttg
       61 gtgattacgg tcatggcggt gatcggagcg ttgcggggat agcttagagc atgcttccac
      121 tcctccttgc ggcggccatc ctcactcaga cgcctgacga tggccagtgg acgatgcccg
      181 ggaagaattt cgccgctacc cgtttcagct cgctcacatc gatcaccacc tccaacgccg
      241 cgcagctccg ccccctctgg agcttctcga ccggcgtcct cggcggccat gaaggccagc
      301 ccctggtcgt gaacaacacg atgtacgtgg tgacgccatt ccccgacgtg ctctatgctt
      361 tcgatctcac caaagacggc taccccctga aatggaagta ccgccccgac gtgaacccct
      421 cggcgctcgg caccgcctgc tgcgacgcga tcaaccgggg accggtctac gcggacggca
      481 agatcgtcta caacctgctg gacggtcaca ccgtcgcggt cgacgcgacc acgggccaag
      541 agctctggaa ggtccaggtc gccgacctcg gccagggtga gacgatgccg atggcgcccc
      601 tcgtcgtgag ggacctggtc ttcgtggggc cgtcaggggg ggaattcgga atccacgggt
      661 ggctgaaggc cctcgatctg gccaacggga aggtcgtctg gaccgcctac aacgagggtc
      721 cggacaagga catgctcatc gacacgagca agttccatcc cttctacgat cacggcaaag
      781 acctggggct caccacttgg cccggagacg cgtggcagcg cgcgggaatc ccggtgtggg
      841 gctggctgtc gtacgacccg gatctggatc tcctctatta cggcaccggc aacgcagccc
      901 cctacaatcc tgagcagcgc gccggggaca acaagtgggc gtcgagtgtt ctggcgcgcc
      961 ggccccggga tggcgcgctg gtctgggcct atcaattcac accgcacgag aactgggact
     1021 acgacgccaa cgccgaaatg atcctggcgg atctgaacat cggcggaaag gtgcgccacg
     1081 cgctggtgca cttcgacaag aatggcttcg cctataccat cgaccgcgaa accggcgagg
     1141 tgctggtcgc gaagccgttc acgacggtga cctgggccaa gagcatcaac ctgaagaccg
     1201 gtcgccccgt cctcgactcg gccaagctca ccggcgcgtc gcggggaaac gtcaaggaca
     1261 tctgcccctc actggagggc gggaagagcc ccgtgtccgc gggctcgtac tcgccgcaca
     1321 ccgagtactt ctacgtcgcg acgaacaacc tgtgcatgga ctaccaggcc tcccaggtgt
     1381 cacacatcgc gggcacgccg tacatcgggg cgaatacacc gtaccatgcg ggacccgggg
     1441 gatacatggg agagttcatc gcgtgggatg cggtgaacgg gaagaaggtc tggggcatta
     1501 ccgagaagta cccggtgtgg agtgggtcgg tgacgacggc gggcgacgtt gttttctacg
     1561 gcaccttgga cggatggttc aaggccgcga acgcctggac tggccaagtg ctctggaagt
     1621 tcaaagtggg ctcgggcgtc gtgggatgcc ccatcgtctt caagggcgcc gacggcaaac
     1681 agtacgtagc ggtgtatgcg gggatcgggg gtgactggtt cctcttgtcc ggggacgtcc
     1741 gctccgatga tcccgcggat gtccgtcccc ccgcggattt cgccaaggac ttggcgcgtc
     1801 acacgagcca aggtggactg gtatggatct tcggcttgcc ctgatcggcg cgacggccct
     1861 gggcgcggtg gccgttgcct gcaaaccggt gactcccgcc aacaccggcc gctacgtccc
     1921 gcccgtgatc tcgtatccgc agcacgtgaa cgtcgccggg atcgcgccgg ccgcgggcac
     1981 gctcaccaat ccgtacgagc cggacagtgc gtcggtgcgt gagggtggcc agacctttac
     2041 ggcgatgaac tgcgacggct gtcatggcgg cggcgccacc ggttgggtgg ggccgagtct
     2101 ggtggatggg cggtggaggt acggcgggga ggatggggcg gtgttcacct cgatcttcta
     2161 cggccgcccc cgcggcatgc cggcgtacgg cgggacactc tcgcccgctg ccatctggaa
     2221 gatcgtgacc tacatccgcg cgcaacccgt cccgacggac gttcccaccg agcgatggta
     2281 agcgaagtct gcgttccgaa catcgggccc cgggaacggc ggaagcggct ctatgccggg
     2341 ttcggctacc tcggcctagc cattgtcgca ctcgcgatca tgacggccac gggtaccccg
     2401 cgcgggtatc gtcttcttct cttcctgcca ctttgggccg cgggtgtggg ggtttttcag
     2461 tacctcgaga agacgtgaat tcgcctagcg gcgcgcggtc tgcgcgacct cgatacggga
     2521 cggcagacga tgacggatcg gcacgaactc gagcgggtca aggagcaggc ggtgcgggtg
     2581 tacgtgaaga ccacgatcgc cgcggcggtg gtcgtggcgg taagttattt tatctgagcc
     2641 agttacctga cgcctgacac ctggcccccg acccctcgta gtgttcctca gtccccaact
     2701 cgagccctaa atgctgaagc cagtcctgca gctcgccgcc gttggagtcg tgggcgtagt
     2761 gttgttcaag ctcgccgcgc tcctgttcct gcccctcctc gccatc
//
LOCUS       DASUBP010000104         8283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_656795, whole genome shotgun sequence.
ACCESSION   DASUBP010000104 DASUBP010000000
VERSION     DASUBP010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 8283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8283
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_656795"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VFU75_03800"
     CDS             complement(<1..620)
                     /locus_tag="VFU75_03800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465986.1"
                     /translation="MRLGLSLLLAAPLAGQERVVPRVGLVITHSVRLTPGTWHLTPSG
                     SLDSGVITIRGDSITVDARGVTLLGASDTTLPDEYRGVAIRVEGGHGVRILGAAIHGY
                     RFGVLAKGTHGLFLDGLDVSRSWKPRLFSLVEHESLVDWLSFHHNENGEWLRYGAGIY
                     LESVDSGDIKNVTGEGGMNGLMLVRSDHLRIWDNDFSFNSGLGIGL"
     gene            complement(680..928)
                     /locus_tag="VFU75_03805"
     CDS             complement(680..928)
                     /locus_tag="VFU75_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465987.1"
                     /translation="MTMLSVAASMDASLKRRSDIDDDFEDFEEDKDLDEDFDDELDEE
                     DEDEDSDDLDDYDDLDDEFDDDEDDEFRPKKGARRDWE"
     gene            complement(962..2809)
                     /gene="typA"
                     /locus_tag="VFU75_03810"
     CDS             complement(962..2809)
                     /gene="typA"
                     /locus_tag="VFU75_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017980090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEU5465988.1"
                     /translation="MAIRNIAIIAHVDHGKTTLVDQMLRQAGVFRANQQVAERVMDSN
                     PLERERGITILAKNTSVRWGDTKINIVDTPGHADFGGEVERILRMVDGFLLLVDAAEG
                     PMPQTRFVAGKALALGLRPIVLVNKIDRQDAEPMRVHDEVLELFLDLEANQDQFTCPF
                     LYTSSRAGTATHELKEPGTSLEPLFQTILDTIPAPKANAGGPFQMLISTLDYSSYLGR
                     IGIGRIERGRVHVGDTVAVLPLGDPGPVSDGPYEQAKVVKLFAFEGLERKDVTEAEAG
                     EIVAISGLPGVEIGKTVTAPDHLERLEGIAVEEPTISVDMLVNNSPFAGREGKFVTAR
                     QVRERLYKELERNVALRVEDTDNTYTFTVSGRGELHLGILMETMRREGYEFQVSRPRI
                     IPREGPNGEQLEPYEELMVDCPEGYLGVVMEKLGPRRATLMDMKNPGVGTVRMRFKIP
                     ARGLLGYRSEFLTDTRGTGIIHHRFLEYDTWAGPLSGRSRGVMVADREGVAVGYALFN
                     LQERGTMFVKPGDPVYEGMIVAENVRPGDMDVNATKEKKLTNMRTTAADEMIILEPPR
                     QVTLELALEYIEDDELIEVTPSSIRLRKRALGANERKKLARADKRAEES"
     gene            complement(2887..3789)
                     /locus_tag="VFU75_03815"
     CDS             complement(2887..3789)
                     /locus_tag="VFU75_03815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEU5465989.1"
                     /translation="MAAPAALPIDATCVHHAPNRHESARRMGWVLGITAAVMVAEAVG
                     GWASHSLALLADAGHMLADVASLGLAVVVARLAQRPATAERTYGLLRLEVFAAFINGA
                     VLLVIAVGIGVEAFRRFSAPPHIHAGILLVIAGIGLAANLVSAKILHHGHDHSLNQRG
                     AYLHVLSDVLGSVGAIVAGVVVIATGWTLADPIISVGIGLLIVNGSWRLVKESVDVLL
                     EATPAHIELPHVHDQIANIPGVTSVHDLHVWTVTSGVVAMSGHLVVRNPADNQRILEA
                     VQERMEKLKIRHVTVQMEKDPTCE"
     gene            3820..5166
                     /locus_tag="VFU75_03820"
     CDS             3820..5166
                     /locus_tag="VFU75_03820"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HEU5465990.1"
                     /translation="MPEPLLSTIGLRLAAVLALVLANAFFVAAEFALVASRRTRIDAM
                     IAGGDTRARTVKQALQTIDRTISGTQLGITLASLGLGWIGEPAIADVLAALFGTLRAP
                     FNVIATHSVAVAVAFVLITFLHIVLGELAPKGLALLHPEQMSRWLTGPLMVFTTATNP
                     FIWLLRSSANGILRLLGARKPSENQRVHSPEEILMLVEQSGRAGSIDREDARLIEGVF
                     EFSEKNAREVMTPRTDIVALEADQTVEAAADTVAKAGRSRYPVTGESLDDIVGVVHAK
                     DILRALRSGGNQPVSSVARPAFFVPGTREVEDVLADMKRKKVHLAVVLDEFGGTAGLV
                     TMEDLLEEIVGQIYDEYDRSEPTPAGAGSVIAGATPVADVNESYGLSLDPGDYTTIGG
                     LVFGALGRLPKVGDQIRLPGVTFEVVTMEGRRVGQLRVQPGAPPPPPPPPPGRPLV"
     gene            complement(5163..5972)
                     /gene="pdxA"
                     /locus_tag="VFU75_03825"
     CDS             complement(5163..5972)
                     /gene="pdxA"
                     /locus_tag="VFU75_03825"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: protein motif:HMM:TIGR00557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HEU5465991.1"
                     /translation="MLVGPDDLLSRIPARSRLGVGDWGLGIGDSEDPERELIAGRLAA
                     RSIERAVWLARAGEVDAVVTAPVHKAALHRAGYRYPGVTEFLGALTGGAETVMMLVTG
                     ALRVALVTTHLPLREALGQITTDRVVRVGETVLAALKDRFGIPAPRLALCALNPHAGE
                     GGLFGDDDQRILAPAAQRLGAFGPFPADTVFVRALKGEFDAVLAPYHDVGMTAVKVAG
                     FGSGVNVTLGLPFVRTSPDHGTAFDIAGTGKADAGSMRAAIDLAARMARRS"
     gene            complement(6076..7380)
                     /locus_tag="VFU75_03830"
     CDS             complement(6076..7380)
                     /locus_tag="VFU75_03830"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU5465992.1"
                     /translation="MTAILLAAALAVMAVPAALPAQVPLDRIVAVVGDHPILASEVEE
                     RVLVSAQQGQIPADSAARAALRGRILQQMINEELEVQQALRDTSIKVTDQEVQDQVES
                     QVKSVKAQIPDPNEFLRQLHIAGFTTVEEWRRKVAEDQRRQILVSRLLENLRQNDKLR
                     PIPPNDAQMRAFWDLHKNEVGKRPANISYRQIVILPQPDSAARAAALHLADSLYQALR
                     AGADFPTLAKKFSADSASAVQGGELGWFRRGVMVKPFEDAAFKLKPGDISPPVETKFG
                     FHVIQVERLQPAEVLARHILIVPVISAAQIQKARRLADSVYTLWKNGASFDSLAARYS
                     DPDEQKLVENEPLTGQTPIAAEYVTAVTQDTTMGIKPVIVVGANSPRPKFAVIEVTGR
                     QAAGDVTFDDVKETIKSRVASDLAEAHYIDQLRHATYVDVRN"
     gene            complement(7408..>8283)
                     /locus_tag="VFU75_03835"
     CDS             complement(7408..>8283)
                     /locus_tag="VFU75_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465993.1"
                     /translation="PPFEVPAWKLAPGEMSGVVQTPYGFHIIRRPPLSEIRDSFALNV
                     QQGIDETFDSLYNARLRSQRHLTIIKSSLASARAALQDADAAENNTTKLVTYDGGDFR
                     IKDLVRWLHALDPQLASSVPGATDAQLSTVLETLTTRDILLQQADSAHVALNADDWQS
                     IKAAYDTTIVHLEEAIQLSPQLLHDSSASPDGRAAIAAAHVNQYLSDVVSRRRGFLGV
                     PPFLATVLRASAPWSLNSAGIQRATEQAQADRVAAGGGTSGPGTGGQPPLRPAPGPAP
                     APQATPVKPPAQGAH"
BASE COUNT         1354 a         2823 c         2731 g         1375 t
ORIGIN      
        1 tagagcccga tgccgagccc ggaattgaac gagaaatcgt tgtcccagat gcgcagatgg
       61 tcagaccgga cgagcatgag cccgttcatg ccgccctcac ccgtcacgtt cttgatatcc
      121 ccggaatcca cgctttcgag atagatcccc gccccatacc ggagccactc gccattctcg
      181 ttgtgatgga acgagagcca gtcgaccagg ctctcgtgct cgaccaggga gaacaagcgg
      241 ggtttccagc tgcgggagac gtcgaggcca tcgaggaaca gcccgtgggt gcccttggcc
      301 aggacaccga agcggtaccc atgaatcgcc gcccccagaa tcctgacgcc atggcctcct
      361 tccacccgga tcgcgacgcc ccggtattcg tcgggaagcg tggtgtcgga ggcgccgagg
      421 agggtgacgc cgcgggcgtc gacggtgatc gagtctccgc ggatggtgat gactccggag
      481 tccagggagc cggaaggggt caggtgccag gtgccagggg tcaggcgaac cgaatgggtg
      541 atgacgagac ccacccgggg cacgacccgc tcctgtcctg ccagcggggc cgccaggagc
      601 aggctcagcc ccaaacgcac gagggccccg cgggtgacag ctcccccgcg aggcccttca
      661 ggtccgaccc ggtcaggtgc tattcccaat cccgccgagc ccccttcttg ggacggaatt
      721 cgtcgtcctc gtcgtcgtcg aattcgtcgt ccaggtcatc gtagtcgtcg aggtcgtccg
      781 aatcctcgtc ttcatcctct tcgtccagct cgtcgtcgaa gtcttcgtcg agatccttgt
      841 cctcttcgaa gtcctcgaaa tcgtcgtcga tgtcgctgcg acgcttgagc gacgcgtcca
      901 tcgacgctgc cacggacagc attgtcaccc ccggctagta gtggtgggac gggaacgagc
      961 gttaggactc ttcggcccgt ttatcggcgc gggccagctt cttccgttcg ttggctccga
     1021 gtgcccgctt gcgcaggcgg atggacgacg gcgtcacctc gatcaactcg tcatcctcaa
     1081 tgtactccag cgccaactcc agtgtgacct gccgcggcgg ctccaggatg atcatttcgt
     1141 cggccgccgt cgtgcgcatg ttcgtcagct tcttttcctt cgtggcgttc acgtccatgt
     1201 cgcccggacg gacgttctcg gccacgatca tcccttcgta cacggggtca ccaggtttca
     1261 cgaacatggt accgcgttcc tgaagattga agagggcgta tcccaccgcc accccctccc
     1321 ggtccgctac catcaccccc cggctgcggc ccgacaaggg gcccgcccag gtatcgtact
     1381 ccaggaaacg atgatggatg attcccgttc cccgcgtatc cgtcaagaac tccgaccggt
     1441 accccagcaa accccgggcc gggatcttga agcgcatccg caccgtcccc accccggggt
     1501 tcttcatgtc catcagggtg gcccgccgcg gccccagctt ctccatcacc acgcccaggt
     1561 acccctcggg gcagtccacc atcagctcct cgtacggctc gagctgctcc ccgttgggcc
     1621 cctcgcgcgg gatgatccgg gggcgcgaca cctggaactc gtacccttca cggcgcatcg
     1681 tctccatcaa gatgccgagg tgcagttcac cccggcccga cacggtgaag gtatacgtgt
     1741 tgtcggtatc ctccacccgg agcgcgacgt tccgctccag ctccttgtag agtcgctccc
     1801 gcacctgccg ggccgtcacg aacttccctt cccggcccgc gaacggcgaa ttgttgacca
     1861 gcatgtccac cgagatcgtc ggctcctcca ccgcgatgcc ctcgaggcgc tcgagatggt
     1921 cgggggccgt gacggtcttt ccgatctcga ccccggggag ccctgagatt gcgacgatct
     1981 cgccggcctc ggcctcggtc acgtctttgc gttccaggcc ttcgaatgca aagagcttga
     2041 cgaccttcgc ctgctcgtag ggaccgtccg aaaccgggcc cggatccccc agtggaagta
     2101 cggcgacggt gtcgccaacg tggaccctcc cgcgctcgat gcgcccgata ccgatacgac
     2161 ccagatagga cgaatagtcg agcgtggaga tcagcatctg gaacggcccg ccggcgttcg
     2221 ccttcggagc cggaatggtg tcgagaatgg tctggaacag gggctcgagc gaggtccccg
     2281 gctccttcag ctcgtgcgtc gccgtgcccg cgcggctcga cgtatagagg aaggggcagg
     2341 tgaactgatc ctgattggcc tcgaggtcga ggaagagctc gaggacctca tcgtgtacgc
     2401 gcatcggctc ggcgtcctgc cggtcgatct tgttcaccag cacgatgggc cggagtccga
     2461 gcgccagtgc cttccccgcg acgaaccgcg tctgcggcat cgggccttcg gcggcgtcga
     2521 cgagcagcag gaacccatcc accatgcgga ggatgcgctc gacctcaccg ccgaagtcgg
     2581 cgtgcccggg ggtgtcgacg atgttgatct tggtgtcgcc ccaccgcacc gaggtgttct
     2641 tggccagaat cgtgatgccg cgctcccgct cgagcgggtt cgagtccatc acgcgctccg
     2701 cgacctgctg gttggcgcgg aacacgccag cttggcggag catctggtct accagggtgg
     2761 tcttgccgtg gtcgacgtga gcgatgattg cgatgttgcg gattgccaag gaattacgtc
     2821 ctaaactggg gccgatttgg agggggaagt gtaagggggg ggtgcgttga aaaacaagcc
     2881 gtcaagtcac tcgcaggtcg gatccttctc catctgcacg gtgacgtggc ggatcttcag
     2941 cttttccatg cgttcctgca ccgcttccag aatccgctga ttgtccgcgg ggttccgcac
     3001 caccaggtgc ccgctcatgg ccaccacccc gctggtgacg gtccagacgt gcaagtcatg
     3061 aaccgacgtc accccgggga tgttcgcgat ctggtcgtgg acatgtggca gctcgatgtg
     3121 tgccggcgtg gcctcgagga gcacgtcgac gctctccttc accagccgcc acgacccgtt
     3181 cacgatcagc agcccgatcc ccacggaaat gatcgggtcg gccagggtcc agcccgtcgc
     3241 gatcacgacc acgcccgcga cgatcgcgcc caccgagccc aacacgtccg acagcacgtg
     3301 gaggtacgcg ccgcgctggt tcagcgaatg gtcgtgtcca tggtgaagga tcttcgcgct
     3361 gaccaggttc gccgccagcc cgatccccgc gatgaccaga aggatgccgg cgtggatatg
     3421 gggtggcgcg gaaaaacgcc ggaaggcctc gaccccgatg ccgaccgcga tgacgagcaa
     3481 caccgcgccg ttgatgaacg cggcgaacac ctccagccgc agcagaccgt aggtgcgttc
     3541 cgccgtcgcc ggccgctgcg cgagacgcgc cactaccacc gccaacccca gcgaggccac
     3601 gtccgccagc atgtgaccgg cgtcggcgag gagtgccagc gagtgggacg cccagccccc
     3661 caccgcctcc gccaccatga ccgccgccgt gatgccgagg acccaaccca tccggcgcgc
     3721 actttcgtgg cgattgggcg cgtggtggac gcaggtggca tcgataggca gggcggcagg
     3781 cgcggccatg tcggaaaaca tatagctttg gcgcccggta tgccggaacc cctgctctcc
     3841 accatcggcc tccgcctggc tgccgttctg gcgctcgtcc tcgcgaacgc gttcttcgtc
     3901 gctgccgaat tcgcgctggt cgcttctcgg cggactcgca tcgacgccat gatcgccggt
     3961 ggggacacgc gcgcccgcac ggtgaagcag gcgcttcaga ccatcgaccg taccatctcc
     4021 ggtacccagc tcggcatcac gctggccagc ctcgggttgg gctggatcgg cgagccggcg
     4081 attgcggatg tgctcgcggc gctttttgga accctgcggg cgcctttcaa cgtcatcgcc
     4141 acgcactcgg tggccgtggc cgtcgcgttc gtcctcatca ccttcctgca catcgttctc
     4201 ggcgagctgg ccccgaaagg cctggccctg ctccatcccg aacagatgag ccggtggctc
     4261 accgggcccc tgatggtgtt cacgaccgcc accaatccct tcatctggct tctgcgctcc
     4321 tccgcgaacg gcatcctgcg cctgctcggc gcgcggaaac cctccgagaa ccagcgcgtg
     4381 cattctccgg aggagatcct gatgctggtc gagcagagcg gcagagcggg cagcatcgac
     4441 cgcgaggatg cccgcttgat cgagggcgtg ttcgaattca gcgagaagaa cgcgcgcgag
     4501 gtgatgacgc cgcgcaccga catcgtggca ctcgaggcgg accagacggt ggaagcagcc
     4561 gcggataccg tggcgaaggc cggccgctca cgctaccccg tcaccggcga gtcgctcgac
     4621 gacatcgtgg gcgtggtcca cgcgaaggac atcctccgcg cgctgcgctc cggcggcaat
     4681 cagccggtat cgtcggtggc acgccccgcc ttcttcgtcc ccggcacccg cgaggtggag
     4741 gatgtgctcg cggacatgaa gcgcaagaag gtccacctgg ccgtggtact cgacgagttc
     4801 ggcggcaccg ccggcctggt cacgatggag gatctgctgg aggagatcgt cgggcagatc
     4861 tacgacgagt acgaccgctc ggaacccacc ccggccggag cgggatcggt gatcgcgggc
     4921 gcgacgccgg tcgccgacgt gaacgagagc tacggactga gcctggatcc cggggactac
     4981 acgaccatcg gtggtctcgt gttcggagcc ctcgggaggc ttcccaaggt cggggatcag
     5041 atcaggctgc ccggcgtaac cttcgaagtg gtgacgatgg aaggcagacg ggtaggtcaa
     5101 ctccgcgtgc aaccgggggc accccctccg cccccgcccc cgccccccgg aagacccctc
     5161 gtctagctcc ggcgcgccat ccgcgcggcg aggtcgatcg ccgcgcgcat cgagcccgca
     5221 tccgccttgc cggtccccgc gatgtcgaaa gccgtgccat gatcggggga ggtgcggacg
     5281 aaggggagcc cgagcgtcac gttcaccccg ctcccgaacc cggcgacctt cacggccgtc
     5341 atgccgacgt cgtgataggg cgccagcacg gcgtcgaact cccctttgag tgcccgcacg
     5401 aacaccgtgt ccgccgggaa cgggccgaag gcgcccagcc gctgggccgc gggagccagg
     5461 atccgctgat cgtcgtctcc gaacaggccg ccctcgccgg catgcgggtt cagggcacag
     5521 agcgccagcc tcggcgcggg aattccgaag cggtccttga gcgccgccag caccgtctct
     5581 cccacacgga ccacgcgatc ggtggtgatc tgccccagcg cttcgcggag cggcaggtgc
     5641 gtcgtgacga gcgccacccg cagcgcgcca gtcacgagca tcatgaccgt ttcggcgccg
     5701 ccggtgagcg cgcccaggaa ctccgtcaca ccgggatagc ggtagccggc ccgatgaagg
     5761 gcggccttgt gaacgggcgc ggtgacgaca gcgtccactt ccccggcccg ggcgagccag
     5821 acggcccgct cgatggaccg ggccgcgaga cgcccggcaa tcagctccct ctccggatcc
     5881 tccgaatccc caatccccaa tccccaatcc ccgactccca ggcgggaccg ggccggaatt
     5941 ctcgagagca ggtcgtccgg cccgacgagc acgatgtcgg cggcgggagg ggcagccgcg
     6001 agggcctgcg ctacgacttc aggcccgatg cctctcgggt caccgagcgt gagggccagg
     6061 cggggacggg aagaactagt tgcggacgtc gacgtacgtg gcgtggcgaa gctgatcgat
     6121 gtagtgcgct tcggcgagat ccgacgcgac ccggctcttg atcgtttcct tcacgtcgtc
     6181 gaaggtgacg tcacccgcgg cctggcgacc ggtcacctcg atgaccgcga acttggggcg
     6241 cggcgaattg gcaccgacga cgatgacggg cttgatcccc atcgtggtgt cctgcgtaac
     6301 ggcggtgacg tactccgcgg cgatcggggt ctgcccggtg agcggctcgt tctccaccaa
     6361 tttctgctcg tccggatcgg aatacctggc ggccagcgaa tcgaacgacg cgccgttctt
     6421 ccacaacgtg tagaccgaat cggccaggcg gcgcgccttc tgaatctggg ccgccgagat
     6481 gacgggaacg atcagaatgt gccgagccaa cacctcggcc ggctgcagcc gctccacctg
     6541 aatgacgtgg aacccgaact tcgtctccac cggcggagag atgtcgccgg gcttgagctt
     6601 gaaggccgcg tcctcgaacg gcttcaccat gaccccgcga cggaaccagc cgagctcccc
     6661 gccctgcacc gccgaagcgg agtcggcgga gaacttcttg gcgagggtgg ggaaatcggc
     6721 gccggcgcga agggcctgat acagggagtc ggcgaggtgg agggccgcgg cccgcgcggc
     6781 cgaatcgggc tgcggcagga tcacgatctg ccggtaggag atgttcgccg gccgtttccc
     6841 gacctcgttc ttgtgcaggt cccagaaggc gcgcatctgg gcgtcgttcg ggggaatggg
     6901 gcgcagcttg tcgttctggc gcaggttctc gagcagacgc gacaccagga tctgccgccg
     6961 ctgatcctcg gcgaccttcc gccgccattc ctcgaccgtg gtgaagccgg cgatgtgcag
     7021 ctggcgcagg aactcgttgg gatcggggat ttgcgccttc accgacttca cctggctctc
     7081 gacctgatcc tgcacttcct gatcggtgac cttgatgctc gtgtcccgta gggcctgctg
     7141 cacctccagc tcctcgttga tcatctgctg caggatgcgc ccgcggagcg cggctctcgc
     7201 cgcggagtcg gcgggaatct ggccctgctg ggccgacacc aggacgcgct cctctacttc
     7261 agacgcgagg atcggatgat cacccaccac cgcgacgatg cggtcgagcg gcacttgcgc
     7321 cggcagcgcc gccgggacgg ccatgacggc cagcgcggcc gccaggagga tggccgtcat
     7381 cagcgacggt gaagggcggc gacccaccta gtgcgctccc tgagccggcg gtttcaccgg
     7441 agtcgcctgg ggcgcggggg ccgggcccgg agccgggcgc aggggcggct gaccgccggt
     7501 gccggggcca gacgtgcctc cgccggccgc gacgcggtcg gcctgcgcct gttccgtcgc
     7561 ccgctggatg cccgcggagt tcaggctcca cggagcggag gcgcgcagca cggtcgcgag
     7621 gaagggcggg accccgagga agccacggcg acggctcacc acatccgaaa ggtattgatt
     7681 gacgtgcgcg gccgcgatgg cggcccgccc gtcgggcgaa gcgctggaat cgtgcaacag
     7741 ctggggcgaa agctggatcg cctcctcgag atggacgatc gtggtgtcgt aggcggcctt
     7801 gatggactgc cagtcatccg cgttcagcgc cacgtgcgcc gagtccgcct gttgcaggag
     7861 aatgtcgcgg gtggtgagcg tctccagcac ggtcgagagc tgcgcgtcgg tcgcgccggg
     7921 gaccgacgag gccagctgcg gatcgagggc gtgaagccag cggaccagat ccttgatccg
     7981 gaaatcgccg ccgtcgtagg tcacgagctt ggtggtgttg ttctccgcgg catcggcatc
     8041 ctggagggcc gcccgcgcgg aggcgaggga gctcttgatg atggtgaggt gccgctgcga
     8101 gcggaggcgc gcgttgtaga gagaatcgaa ggtctcgtcg atcccctgct gtacgttcag
     8161 cgcgaacgaa tcgcggatct ccgagagggg aggccggcgg atgatgtgaa agccgtacgg
     8221 cgtctgtacc acgccgctca tctcccccgg cgcgagcttc cacgccggca cttcgaacgg
     8281 cgg
//
LOCUS       DASUBP010000105         2599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_643256, whole genome shotgun sequence.
ACCESSION   DASUBP010000105 DASUBP010000000
VERSION     DASUBP010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_643256"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="VFU75_03840"
     CDS             <1..933
                     /locus_tag="VFU75_03840"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein"
                     /protein_id="HEU5465994.1"
                     /translation="QDVAFVQALDVRTPQVSIQAKLIFVDRTDVEDMGVKYDLGTGSG
                     CLITGAHPCQFFNTLVQRADPSTLKPIDTNGDGIPDAVVATGTLPASQTVVDLGGNAL
                     SALGNAGQEVINPALSLIFSTAVGNFNLTSFVQALQTVELADLQAEPSVTTSDNHTAE
                     VLVGDKVPLRVIDVGSIGQSGSAAPRATINFQQTGIDLKVTPHVTANRQILMEVHAEN
                     SSVKTAPVDIGYTIQTQQADNQILVNDGETAVIGGLTVTNVTVSKSGIPFLVDLPILG
                     KLFGFTNQQEERRDLLILITPHIIDDLATTSAGN"
     gene            947..>2599
                     /locus_tag="VFU75_03845"
     CDS             947..>2599
                     /locus_tag="VFU75_03845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465995.1"
                     /translation="MFAGRNVRLYAAVAFGAIVLGGCETAANTGGVSRDNISPRLTLT
                     TPNKVTPKVDSQTITGGLSFNVSASDNIAVDSVDLTFSIGLIARHDTGFAGGAKTVSY
                     VFSLSSAQLAGAGGQILIVGTARDGAGNRSKPDTITIFLSNKQALAAFLLAPTAGAVA
                     GSGKYIPIQVKAVQQAGGIRRLGWTIGGVAGQTATTADSISTGSAPFPDSTIFVDSVL
                     VTGTTGTFQVVGFAVDSAGRRAASAAVTVTIQSVATDNTPPEISQVVALRAEATDSIT
                     IHATDPSGITRVGFKVTDLLGAPLGGDSLNFAGTTTDQKVTMSLKLTNIVVFPTQVIV
                     QAFAIDGATALNRGVTGAVFTVPSPPGASRSDTVTIVAGRTFALPAGGQIGDAIYNKN
                     TNEVYLTNTSLSRLEIFQVANSTFVAAGIPTSGPQPVGLALWPSDTLGNYQDTVVVAN
                     SGGTELSILDTRPAVRALAYRQPLPNFLIEKYQIQNVGGFFQAVITYYDVSDRPQYVG
                     TTCRAAGGTACDPNQIYAIYSTTPTVSSSDPFSGRATLREEKL"
BASE COUNT          456 a          919 c          810 g          414 t
ORIGIN      
        1 caggacgtcg ccttcgtgca ggcgctggac gtccgcacgc cgcaggtctc gattcaggcg
       61 aagctgatct tcgtcgaccg caccgacgtc gaggacatgg gcgtgaagta cgacctcggc
      121 accggctccg gctgcttgat caccggcgcg catccgtgcc agttcttcaa tacgctggtg
      181 cagcgtgcgg atccctcgac cctcaagccg atcgacacca acggtgacgg cattccggac
      241 gcggtggtcg cgaccgggac cctgcccgcc agccagacgg tggtggacct cggcggcaac
      301 gccctgtcgg cgctcggcaa cgcgggccag gaagtcatca acccggccct ctcgctcatc
      361 ttctcgaccg cggtcggcaa cttcaacctg accagcttcg tgcaggcgtt gcagacggtc
      421 gagctcgccg acctgcaggc cgagccttcg gtgaccacgt cggacaatca cacggcggaa
      481 gtgctggtgg gtgacaaggt gcccctccgc gtcatcgacg tcgggtcgat cggccagagt
      541 ggatcggcgg cgccccgtgc aacgatcaac ttccagcaga ccggtatcga tctgaaggtg
      601 acgccgcacg tgacggcgaa ccggcagatc ctgatggaag tccacgccga gaattcgagc
      661 gtgaagaccg ccccggtcga catcggctac acgattcaga cccagcaggc ggacaatcag
      721 attctcgtca acgacggcga aacggccgtt atcggcgggc tgacggtgac caacgtcacg
      781 gtaagcaagt cgggaatccc gttccttgtg gacctgccca ttctgggcaa gctgttcggg
      841 ttcacgaatc agcaagagga acggcgcgat ctgctgatcc tgatcacgcc ccacatcatc
      901 gacgacctgg cgactaccag cgccgggaac tgaggcgggg gttcctatgt tcgcaggacg
      961 aaacgtgcgt ctgtacgccg cggtggcctt tggagcgatc gtgctcgggg gctgcgaaac
     1021 ggcggccaac accggcggcg tctcgcggga caacatctca ccgcggctca cgctgacgac
     1081 gccgaacaag gtgacgccca aggtcgacag ccagaccatc accggcggcc tctccttcaa
     1141 cgtctcggcc tcggacaaca tcgcggtcga ctcggtcgac ctgaccttca gcatcgggct
     1201 catcgcccgg cacgacaccg gattcgcggg cggggccaag accgtcagct acgtcttcag
     1261 cctcagctcg gcccagctgg ccggcgccgg tggccagatt ctgatcgtcg gcaccgcccg
     1321 ggacggcgcg ggcaaccgct ccaagcccga caccatcacg atcttcctgt ccaacaagca
     1381 ggccctcgcg gccttcctcc tggccccgac cgcgggtgcc gtggcgggca gcgggaaata
     1441 catcccgatc caggtgaagg ccgttcagca ggcgggcggc atccggcgcc tcgggtggac
     1501 catcgggggc gtcgcgggcc agacggcgac caccgccgac tccatctcta cgggctcggc
     1561 tccgttcccc gactcgacca tcttcgtgga ctcggtgctc gtcaccggta cgaccggcac
     1621 cttccaggtg gtcggcttcg ccgtcgactc ggcgggacgc cgtgcggcca gcgccgcggt
     1681 gaccgtcacc attcagtcgg tggccacgga caacacaccc ccggagatct cgcaggtggt
     1741 ggcgctccgg gcggaagcca cggacagcat caccattcat gcgaccgatc cgtcgggtat
     1801 cacccgcgtc gggttcaagg tcaccgattt gctcggggcc cccctgggcg gcgattcgct
     1861 caacttcgcg ggcaccacca ccgaccagaa ggtcacgatg tcgttgaagc tgacgaacat
     1921 cgtggtgttc cccacgcagg tcatcgtcca ggcattcgcc atcgacggcg ccacggcgtt
     1981 gaaccggggc gtgacggggg ccgtgttcac cgtccccagt cccccgggcg cgtcgcgctc
     2041 cgacaccgtg accatcgtgg cgggacggac gttcgccctg ccggccggtg gccagatcgg
     2101 tgacgccatc tacaacaaga acaccaacga ggtgtatctc acgaacacct cgctgtcgcg
     2161 cctcgagatc ttccaggttg cgaacagcac cttcgtcgcc gcgggcatcc cgacgtcggg
     2221 cccgcagccc gtcgggctcg cgctctggcc gagtgacacg ctgggcaatt atcaggacac
     2281 ggtggtggtc gcgaactccg gcggtaccga gctgtcgatc ctcgacaccc ggcccgcggt
     2341 gcgcgcgctc gcctaccggc agccgctgcc caacttcctg atcgagaagt atcagatcca
     2401 aaacgtcggc ggcttcttcc aggccgtcat cacctattac gacgtctcgg accgcccgca
     2461 gtatgtgggg accacctgcc gcgcggcagg cggcaccgcg tgcgacccga accagatcta
     2521 cgccatctat tcgaccaccc caacggtctc gagctccgat ccgttctcgg gccgcgccac
     2581 cctgcgcgaa gagaagctc
//
LOCUS       DASUBP010000106         7315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_970144, whole genome shotgun sequence.
ACCESSION   DASUBP010000106 DASUBP010000000
VERSION     DASUBP010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7315
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_970144"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..2857)
                     /locus_tag="VFU75_03850"
     CDS             complement(197..2857)
                     /locus_tag="VFU75_03850"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU5465996.1"
                     /translation="MTDLGRLTAALSDRYRIERELGAGGMATVYLAHDVRHDRPVALK
                     VLRPELAAVIGAQRFLAEIKTTANLQHPHILSLFDSGEADGTVFYVMPYVEGESLRDR
                     LSREKQLPVDAAIRIATEVADALEYAHQQGVVHRDIKPENILLHGGHALVADFGIALA
                     AAKTGDTRMTETGMSLGTPAYMSPEQAMGEREITPRSDVYALGCVTYEMLVGEAPFTG
                     PTAQAIVAKVLTAEPASLIAQRRTVPDYVEGAVLRALEKLPADRFARAGEFAQALQGA
                     AGMTSRRTTRAASLIPAPHPRTPLFAALAVVLGIAAIWGWSRHTGSGDAPVVRFELRA
                     GAEGVALPFSGVATGVAISPDGRLLAYLGGPSTNWMIYLRDVDQLTARALPGTEGALN
                     QEFSPDGRWIAFRAPDGKLKKISVDGGAIATLCAIDNSGTVGAGIAWLSDREIIFARG
                     TYSEGRGFWKVSSDGGEPTPFSVVDSTTGERLQLSPTVADGGRLVFYSSTVASNIDLN
                     IGVIPTATGKPLVFSTLKGIRAVGLVRGYLVYVRGDGALMAAPFNRSTLTVGSPIQVG
                     DSVAVAPSAWESPVALSASGSLFYQRGGAVGKFVTVDMHGATRPLLDEAGVFLHPRLS
                     PDGRRLAYEVQGGTGSDIWVANLARHTRERLTREGFNNRPEWSPDGSRVLYTSSRAPD
                     NSLWWQPADGSADGTLLYHGAKPIREGVFTPDGRSVIFRWDTQDSNRDILILQLGGDL
                     KPQPLVATVNDEKEPRVSPDSKWLAYMSNENGREEVYVRALSGAGGRVLVSDGGGTEP
                     LWSRDSRTLYYRQGPSLYAVPVTGTPTLGVGARALVFAGPFATDEYHPDYDVTPDGKG
                     FVMILPVAEDRQLLVAINWAQELKARVGTQ"
     gene            complement(2863..4749)
                     /locus_tag="VFU75_03855"
     CDS             complement(2863..4749)
                     /locus_tag="VFU75_03855"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU5465997.1"
                     /translation="MRIALLVSLGLATIPGVDRGARRAAPRPIAFNDNRVSAGTLARG
                     VLTISLVAQPGVWRPYGSTGGAVALLAFGEAGQPLQSPGPLVRVSLGTRIRARVENLT
                     GAPLVVHGLASRLVPAMDSLMIAPGETGLTEFTADAPGTFYYWGSTAGEAFGDRVARD
                     EHLNGALVVDSGPPVPDGDRIFVIERWSPDQIHLGAHELFSINGRPWPYTERLTYDVG
                     DSVRWRVVNASNTIHPLHLHGFYYRVDARGDLARDTLYWPAQRRMNVTERMEPGTTMG
                     MVWHPTRPGGWIFHCHLNYHVPPNTALPPDTETEVERNRLIHEGYPDQHPVDHAMHAM
                     GGLVLGIYVRPPAGWRLPTSARRTLRLLVRSDSGGGERGRGFGYFLQEGNAPVTPEVR
                     RAPGPPIVLHRGEPTRIWVVNQSTAMTLVHWHGLEIESAYDGVAGVSGTVGHTEQPIQ
                     PGDSLEVLVTPPRAGSFMYHTHINDPWQQSRGLYGPLIVLDSAETWHPERDLVFMTGD
                     DSTYAAVLNGGGVLTPLSLEAGVPYRIRLMNITTGSPRVRFVLVRNGAPVQWKPLAKD
                     GYDLPSWQAVPRQAVQLVSIGETYDFQFLSPDTGAVTLELRKADGGVLGTQPLRVVPH
                     TTHP"
     gene            complement(4808..>7315)
                     /locus_tag="VFU75_03860"
     CDS             complement(4808..>7315)
                     /locus_tag="VFU75_03860"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEU5465998.1"
                     /translation="GAGAGAVVYEQAGDIHLYDPAAGTDKKLEIHVTGDFPWAMPHSA
                     NVARSLTAPQLSPTGVRALFEGRGDVYTVPVDKGTWRNLTRSPGVADRTPIWSPDGKR
                     IAWFSDSSGEYQLVVDDQDGVTPARVYALEHPTYFYSPAWSPDGSRILFSDAQTNIWI
                     LDLATGKETHVDQDNYTWPSRSVNPVWSPDSKWIAYNKRLLGSQYHAVFVYSLADGST
                     HQMTDGLSDAIDPTWDRSGKYLLFLASTDFALRSGWLDMSSYDHTLERGIYITVLAAD
                     QPSPLLPETGDEGAKSEGGAHRAGTDSASKKTAGTDSVTVRIDFSGLSQRILSLDVPT
                     RDYDQLKAGAAGEFFYVEHVPNRPDVLHRYKLGDRKVVDFVGATRGQFALSFDGKKLL
                     YALGDGAPGRWAVVDAGGEPPAGGKGMLSLGGLTTDVDPLAEWRQIFNEAWRIERDYL
                     YVANLNGADWPAMKARYQVFLPYVRHRADLTMLLSQMQAELTVGHSFAGGGDLPSSDA
                     EPAALLGADFTVSDGHYRIAKIYTGENWNPDLQAPLSAPGVDVKTGDFLLEVNGRPLA
                     PPESPYAPFIGTAGRQVQIRVGPKASGEGSHLVTVVPIQDESGLRTRDWIESNRRKVD
                     SLSGGKLAYVYVPNTGGGGYTSFNRYYFAQQDKQGAVIDERFNHGGSIADYMVDIMSR
                     QLHGYFNQRLGDHYNAVTAPAAAIWGPKVLLINEMSGSGGDMFPFMFRQMKIGPLIGT
                     KTWGGLVGWGGEPRFVDGGFISAPSTGFYNPDGQWDVENLGVAPDILVEQTAADLDAG
                     RDPQLERGVAEALKMLKEHPVQLKPEPPAPDRVHPKP"
BASE COUNT         1236 a         2499 c         2365 g         1215 t
ORIGIN      
        1 ggcgacgatc ctcgcgggca cccccgcgcc gagctgcacc gggaccagct tcgcgaccgg
       61 cacaccccga tcggtgatgg tcaccgattc acccttctgc acccgcttga tcagtgcact
      121 cagccggttc ttggcgttgg aaatggattc ggatttcata tggctattat agccatgtcg
      181 atatagccta tcaagctcac tgagtcccca ctcgagcctt cagctcctgc gcccaattaa
      241 tcgcgaccag gagctgacga tcctccgcca cgggaagaat catcacgaag ccttttccgt
      301 ccggcgtcac gtcataatcg gggtgatact cgtcggtcgc gaacggccct gcgaacacga
      361 gtgcccgagc gcccaccccc aaggtcggcg tcccggtgac cggcaccgca tagaggctcg
      421 gcccctggcg atagtagagc gtccggctgt ccctggacca taggggctcg gtgccgccgc
      481 catccgacac cagcacgcgc ccacccgccc ccgagagtgc ccgaacgtac acctcctcgc
      541 ggccgttctc gttcgacatg taggccagcc atttcgagtc cggtgagaca cgcggttcct
      601 tctcgtcgtt gacggtcgcg accaggggtt ggggcttgag gtcaccgccc agctgaagta
      661 tgagaatgtc ccggttgctg tcctgtgtat cccagcggaa aatcacggag cgtccgtcgg
      721 gggtgaacac gccttcccgg atgggcttcg ccccgtggta caggagcgtc ccgtccgcgc
      781 tgccgtccgc cggctgccac cagagcgaat tgtcgggcgc ccgggatgat gtgtacagca
      841 cgcgtgatcc gtccggcgac cattcaggcc ggttgttgaa gccctcgcgc gtcagtcgct
      901 cccgtgtgtg ccgggccagg ttggcaaccc agatgtccga gccggtccct ccctggactt
      961 cgtaggccag gcgccggccg tccggggaaa gccgcggatg caggaacacg cccgcctcat
     1021 cgagcagtgg ccgcgttgcg ccatgcatgt ccacggtgac gaacttgccc accgcgccgc
     1081 cgcgctgata gaagagcgac ccggaggcgg agagtgccac cggactctcc caagccgacg
     1141 gcgccacggc cacgctgtcg ccgacctgaa tcggtgagcc gacggtgagc gtgctccggt
     1201 tgaacggagc ggccatcagt gcgccgtctc cccggacgta gaccaggtat ccgcgcacga
     1261 gacccaccgc gcggatgcct ttcagcgtcg aaaacaccag gggcttgccg gtcgcggtgg
     1321 gaatcacgcc gatgttcagg tcgatgttgc tggccacggt gctgctgtag aacaccagcc
     1381 gccccccatc agcgaccgtg ggagagagct ggagccgttc ccccgtcgtc gagtcgacga
     1441 ccgagaaggg cgtgggctca ccgccatctg acgacacctt ccagaacccc cgcccttccg
     1501 agtacgtccc gcgggcgaag atgatctcgc ggtcggagag ccaggcgatc ccggcgccca
     1561 cggtaccgct gttgtcgatg gcgcagaggg tggcgatggc cccgccgtcc accgagatct
     1621 ttttcagctt gccatcaggc gcgcggaaag cgatccagcg gccgtccggt gaaaactcct
     1681 gattcaaggc accttcggtc cccgggaggg cgcgggcggt gagctgatcc acatcgcgga
     1741 ggtagatcat ccagttggtg gagggaccgc caaggtaggc caggaggcgc ccgtcgggag
     1801 aaatcgcgac cccggtggca acgccactga aggggagcgc gacaccctcc gctcccgcgc
     1861 gcagctcgaa acggactacc ggcgcgtcac ccgatccggt gtgccggctc cagccccaga
     1921 tcgccgcgat accgagcacc actgcgagtg cggcaaagag aggggtccgg ggatggggag
     1981 cgggaatcag cgacgcggca cgggtagtcc tgcgagatgt catccccgcc gcgccttgga
     2041 gcgcctgcgc gaattcgccc gcgcgggcga agcgatcggc ggggagcttt tcgagcgccc
     2101 gaagaaccgc tccctcgacg taatcgggca cggtcctccg ctgagcgatg agcgacgccg
     2161 gctccgcagt cagtaccttc gccacgatcg cctgcgcggt ggggccggtg aacggggcct
     2221 cccccaccag catctcgtac gtcacgcacc ccaacgcgta gacgtcactc cgcggcgtga
     2281 tctcccgctc tcccatcgcc tgctcgggac tcatgtacgc cggggtgccg agggacatgc
     2341 cggtctcggt catccgggtg tcgccggttt tggccgcggc gagggcgatt ccgaagtcgg
     2401 cgacgagcgc gtgaccaccg tggaggagaa tgttttcggg tttgatgtcc cgatgcacca
     2461 cgccctgctg gtgcgcatac tccagcgcgt cggcgacctc ggtggcgatc cggatcgcgg
     2521 cgtctaccgg cagctgtttt tcgcggctca gccggtcgcg aagggactca ccctcgacgt
     2581 atggcatgac gtagaagacc gtgccgtccg cttccccgga atcgaagagt gacaggatgt
     2641 gcgggtgctg cagattcgct gtcgtcttga tctcggccag gaaccgctgg gcaccgatca
     2701 ccgcggccag ctcggggcgc aagaccttga gggcgacggg ccggtcatgc cgtacgtcgt
     2761 gcgcgagata gactgtggcc atgccgcccg cgcccagctc gcgctcgatg cggtagcggt
     2821 ccgaaagtgc cgcggtgaga cgcccgaggt ctgtcatcca ggtcaggggt gggtcgtgtg
     2881 cgggacgacc cggagtggtt gggtgcccag cacgcctccg tcggcctttc ggagctccag
     2941 cgtcaccgcc ccggtatccg gggacaggaa ctgaaagtca tacgtctcgc cgatgctcac
     3001 gagctgaaca gcctgccggg gcaccgcctg ccaggaaggg aggtcgtacc catccttggc
     3061 gaggggcttc cactgcaccg gcgcgccgtt gcgcaccaac acgaatcgca cgcgggggct
     3121 ccccgtggtg atgttcatga gccggatgcg gtacggaacc ccggcctcga gcgaaagcgg
     3181 agtcagcacg ccgcccccat tcagcactgc ggcgtaggtc gagtcgtcgc cggtcatgaa
     3241 gacgaggtcc cgctccgggt gccacgtctc cgccgaatcg agcacaatca gaggtccgta
     3301 caagccccgg ctttgctgcc agggatcgtt gatgtgcgtg tgatacatga agcttcccgc
     3361 gcgcggcggg gtcaccagca cctcgagcga gtcaccaggc tgaatcggct gttccgtgtg
     3421 ccccacagtc ccgctcactc cggcgacacc gtcgtaagcg ctctcaattt ccagtccgtg
     3481 ccagtgcacc agggtcatcg ccgtactctg gttcacgacc cagattcgcg tcggctcgcc
     3541 gcgatgcagg acgatcggag gcccgggggc ccgccggacc tcgggagtca cgggggcatt
     3601 accctcttgc aggaaatacc cgaagccgcg tccacgctcc cctccgcccg agtccgatcg
     3661 taccagaagt ctcaaggtcc gccgggccga cgtcggcaga cgccatcccg cgggagggcg
     3721 cacgtagatc ccgaggacca gacctcccat ggcgtgcatg gcgtggtcga cgggatgctg
     3781 atcgggataa ccttcgtgaa tgaggcgatt ccgctccacc tccgtctcgg tatccggcgg
     3841 cagggcggta ttgggcggca cgtggtagtt cagatgacag tgaaaaatcc agccgccggg
     3901 gcgggtcgga tgccacacca tccccatcgt ggtccccggc tccatccgtt ccgtgacatt
     3961 cattcgccgc tgggcgggcc agtagagcgt gtctcgggcg agatctccac gcgcatccac
     4021 acgatagtag aagccgtgca ggtgcaaagg atggatcgtg ttggacgcgt tgaccacgcg
     4081 ccaacgcacc gaatccccga cgtcgtaggt aaggcgctca gtgtacggcc aaggacgtcc
     4141 gttgatgctg aacagttcgt gtgcgcccag atgaatctga tcgggactcc agcgctcgat
     4201 gacgaagatc cggtctccgt ccggcaccgg cggcccggag tccacgacga gcgcgccgtt
     4261 caagtgctcg tcccgcgcca cccggtcgcc gaacgcttca cccgccgtac tgccccaata
     4321 gtagaaagtc cccggcgcat ccgccgtgaa ctcggtcagt ccggtctctc ccggcgcaat
     4381 catgagcgaa tccatcgcag gcaccaggcg ggaggcgagg ccgtgcacca ccagcggcgc
     4441 accggtgaga ttctccactc gagcccggat ccgggtgccg agggacacgc ggacgagcgg
     4501 tcccggactc tggagcggct gaccggcctc tccgaacgcc aggagcgcga ccgcgccacc
     4561 cgtggagccg taggggcgcc acacgcccgg ctgggcgacc aatgagatgg tcagcacccc
     4621 gcgggcaaga gtgccggcgg aaacccggtt gtcgttgaag gcgatgggcc tgggcgcggc
     4681 gcgccgcgct ccgcggtcga cacccgggat cgtcgccagg cccagggata cgaggagcgc
     4741 tatccgcatt tcgccgccgg tgaaggtgag cgcccaaggt cccgccccgg acgggccctg
     4801 tcaaccgtta cggcttcgga tgcacccggt cgggagcggg gggctccggc ttcagctgca
     4861 ccggatgctc cttgagcatc ttgagagcct cggccacgcc ccgttcgagc tgcggatcgc
     4921 ggccggcgtc cagatcggcg gccgtctgct ccaccaggat atccggtgcc acaccgaggt
     4981 tctcgacatc ccattgccca tcaggattgt agaacccggt cgagggcgcc gaaatgaaac
     5041 cgccatccac aaaccgcggc tccccacccc atccgaccag tccaccccac gtcttcgtac
     5101 cgatcagcgg tccgatcttc atctgccgga acatgaaggg gaacatgtcg ccgcctgaac
     5161 cgctcatttc gttgatcaag aggactttcg gtccccagat cgccgcggcc ggcgccgtta
     5221 cggcgttgta gtgatcgccg agacgctgat tgaagtaccc gtggagctgg cggctcatga
     5281 tgtccaccat gtagtccgca atgctgccgc catggttgaa ccgctcgtcg atgacggcgc
     5341 cctgcttgtc ctgctgcgcg aagtagtagc ggttgaagct cgtgtacccg ccgccgccgg
     5401 tgttcgggac gtagacgtag gcgagcttgc cgcccgacaa cgagtccacc ttccgacggt
     5461 tcgattcgat ccaatcgcgc gtccggagtc ccgactcgtc ctggatggga accacggtga
     5521 cgagatgtga cccttccccg gatgccttgg gcccgacgcg gatctggacc tgacgcccgg
     5581 ccgtcccgat gaacggcgca tacggacttt ccggcggcgc cagcggacgc ccgttcacct
     5641 cgagcaggaa atcccccgtc ttcacgtcga caccgggcgc cgacaacggc gcctggagat
     5701 cggggttcca gttttcgccg gtgtagatct tggcgatccg gtaatgcccg tccgacaccg
     5761 taaagtccgc gcccaggagc gccgccggct cggcatccga ggagggcaag tctccccctc
     5821 cggcgaagct gtgccctacc gtgagctccg cctgcatctg gctcaacagc atggtcaggt
     5881 ccgcccggtg ccgcacgtac ggcaggaaca cctggtacct ggccttcatg gccggccaat
     5941 cggcaccatt cagattcgcg acgtacaggt agtcacgctc gatccgccag gcttcgttga
     6001 aaatctggcg ccactcggcc agcggatcga cgtcggtggt caagccgccc agggagagca
     6061 tccccttccc gccggcgggt ggctcacctc cggcgtccac cacggcccag cgtccgggcg
     6121 cgccgtcccc cagcgcatac agcagcttct tcccgtcgaa cgagagggcg aattgcccac
     6181 gcgtggcgcc gacaaaatcc acgaccttcc tgtcgccgag cttgtaccgg tggagcacgt
     6241 cgggccggtt gggcacgtgc tccacgtaga agaactcgcc ggctgccccc gccttcagct
     6301 gatcgtagtc acgggtcggg acgtcgagcg acaggatccg ctgcgagagg ccactgaagt
     6361 cgatccgcac cgttaccgag tcggtcccgg ccgtcttctt gctggctgag tcggtccccg
     6421 cccggtgcgc cccaccctcg ctcttcgcac cttcgtcgcc agtctcaggc agcagtgggg
     6481 agggctgatc ggcggccagg accgtgatgt agatcccgcg ctcgagcgtg tggtcgtacg
     6541 agctcatgtc gagccagccc gatcgcagcg cgaagtcggt ggacgcgagg aagaggagat
     6601 acttcccgct ccggtcccac gtgggatcga tcgcgtcgga caggccgtcc gtcatctgat
     6661 gggtggaccc gtcggcgagc gagtagacga acacggcgtg gtactgcgag ccgagcagac
     6721 gtttgttgta ggcgatccac ttcgaatccg gggaccagac gggattcacg ctgcggctgg
     6781 gccacgtgta gttgtcctgg tccacgtggg tttccttgcc ggtggccagg tccagaatcc
     6841 agatgttggt ctgcgcatcc gaaaacagga tccgcgagcc gtcgggagac caggcgggcg
     6901 agtagaaata cgtgggatgc tcgagcgcat acacccgcgc cggcgtgaca ccatcctgat
     6961 cgtccaccac cagctggtat tcaccggaac tgtccgagaa ccaggcgatc cgcttcccgt
     7021 ccggggacca gatgggcgtg cggtctgcca cccccgggga ccgggtgaga ttccgccacg
     7081 tgcctttatc caccggcacc gtgtagacgt ccccgcgccc ttcgaacaac gcccgcactc
     7141 ctgtcggcga gagctgcggc gcggtcagcg agcgcgccac attcgcgctg tgcggcatcg
     7201 cccacgggaa gtcgcccgtc acgtggatct cgagcttctt gtcggtgcct gccgccgggt
     7261 catacaggtg gatgtcgccg gcctgctcgt agaccaccgc cccggcgccg gcacc
//
LOCUS       DASUBP010000107        11361 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_275366, whole genome shotgun sequence.
ACCESSION   DASUBP010000107 DASUBP010000000
VERSION     DASUBP010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 11361)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11361)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11361
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_275366"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VFU75_03865"
     CDS             <1..723
                     /locus_tag="VFU75_03865"
                     /inference="COORDINATES: protein motif:HMM:NF045432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5465999.1"
                     /translation="KSFFQKDAPVFAPEWLRTERMWSEDTQREIDYFVCDDLDSLLYI
                     ANLGTIPLHVWGSRIASLERPDWCILDLDPKEAPFTDVVKVARATKQLCDRIGLPAYI
                     KTSGSTGLHVLIPMGRQVTYEQCRQLGGLLARVVAAELPHIATITRVVGKRGGRVYLD
                     YGQNGHGRLLVAPFSIRPLPGAPVSMPLKWSEVTPALEIGQFTIKNAPARMKKLKRDP
                     VIPVLDEQPDLVKALGLLQARV"
     gene            720..1394
                     /locus_tag="VFU75_03870"
     CDS             720..1394
                     /locus_tag="VFU75_03870"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HEU5466000.1"
                     /translation="MTAKPLSIGVPELGLEVSGLLELPAHARALYVLAHGAGAGMRHP
                     FLERIASGLAAAGMGTLRYQFPYMERGSGRPDTPDKCVATVTAAVASARAEAPALPLV
                     AGGKSFGGRMTSTAQSRAPLDGVRGLIFLGFPLHAPGKPGITRGEHLDLVKIPMLFLQ
                     GTRDEFAQPELLHQVVARLKLRATLHEVEGGDHSFKVPKKQATEASIFEDLVTTITDW
                     TDRSAR"
     gene            1391..1795
                     /locus_tag="VFU75_03875"
     CDS             1391..1795
                     /locus_tag="VFU75_03875"
                     /inference="COORDINATES: protein motif:HMM:NF013995.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HEU5466001.1"
                     /translation="MSYWILKTEPTAYSYDRLEKEKTAVWDGVANPVALKNLKAMEKG
                     DELMIYHTGAEKSVIGTASVAKEAYPDPKAGNPSMVVIDIKVGKRLTKPVTLAQIKAD
                     ATFAEMALVRQGRLSVVPATATQWKRLLALAE"
     gene            1916..3049
                     /locus_tag="VFU75_03880"
     CDS             1916..3049
                     /locus_tag="VFU75_03880"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU5466002.1"
                     /translation="MAFRPEPEPRARFSWKVVLTLWTIYGLTTSLQELVGAMAYGRHE
                     PWWFGPAQMMPQAYLWALLTPFILWWGNRFPPRGPGWPGRVLIHVATSITIVTCMDSA
                     FELYLPYLSAWAHVPLSDLSLPLGSRIIRVLAWWLVFDTMLYWVVLFIGSMRDAGARA
                     QAKAMRESQLETQLAQAELHSLKMQLHPHFLFNALHTVGTLVRTGKSALAVQVVGRLG
                     DLLRRMLDGAATQEVPLREELEFARNYLDVEQARFSDRLKVTWTVEPGAQDAAVPHLI
                     LQPLLENAIRHGISAATASGILIVGARCTNGTLHLTVRDDGPGMADDPGRSVGSSGVG
                     LANTRLRLARLYGDRAGLEVLNAEDGGVTSHVFLPFRPAAPGR"
     gene            3074..3865
                     /locus_tag="VFU75_03885"
     CDS             3074..3865
                     /locus_tag="VFU75_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEU5466003.1"
                     /translation="MRTLIVDDEPAAREGLRHLLSADPEIEVVGECRDGKTATAAIRD
                     QSPELVFLDVQMPELDGLGVLRDVPPERRPVVVFVTGYDRYALQAFEVHAVEYLLKPF
                     TDDRFREALSQAKLQVRRTRMGELGKEMATLAGGANGASAVTEKSAPAAGYRSRLVVK
                     TGGNVVLVPVEDIDWIDADGDYVRIHVGKVSHTVRETMHHLETELDPARFVRIHRSTI
                     VNLARVKELQPFYRGEFVAVLHDGTRLKLSRGCRDTLEARLGRRL"
     gene            4081..7302
                     /locus_tag="VFU75_03890"
     CDS             4081..7302
                     /locus_tag="VFU75_03890"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5466004.1"
                     /translation="MSFSVRSLFAFTSVLVLGGGALAAQTTGKVQGSVTGPDGAPLAH
                     AQVTVTGTSFGAVTDDKGYYFINNIPAGLYRMQAQYIGMAPGQAEGVRILAGQTLTQN
                     FPLQNKALTVTGINVVAAADAAVPRDQVTSKTIVSGELVQNLPVQNVRNVVALTPGVV
                     ESGNGLGVSIRGGRPGEEAVYIDGAPAIRQAQIVGGAMGASPTNLVQTNAVEEASITT
                     GAIAINQGNAQSGVIAVTTRSGGEKLSGAFSAQSDGMFANTISTGYNRFEGSVGGPVP
                     GFSKLRFFLSGVLQGQTSPFRSYGAAEVPSYTMNGVDTTVHVVGPSGAESVTLPKWVQ
                     YSGQCDAAQNSGVSCQGNRLPFDWTTNLNLQGKISYFYGSGSSIALSAIAEGDQNRNY
                     PNQVIGDPALYTGTHTWARDVVLNWDHSIFKQADRALSLNLNLSYATNQSISGVLDPA
                     TEVSTRNPMGGMEFSTLNFGGFGQFAGNFFNNPEQIIRNIRSNDGERVPLLNRTDLNA
                     AQPYRMNPYAMQSGGFFTEGVADAALLNNLYHEQRLYGRAQVDWQANRYHRFNFGGEL
                     QRSDVAAWNGILNSQIFSNAYHTKPTNGAAWVSDRLDLGDVVLEMGVRYDYFNSNALF
                     SNTPGFTFSNPTWNQSSASNDTAYTNSLARTFTPGVGHSTLSPRLQVSFPITDNTDFR
                     LSYSHQVQTPDVNTMLQGINSDLTFTNSNDRFGRDVGFGKTILFEFGVRHAFTPDLVV
                     DLSGYNKSKVADLSYRIFGYNDPTNPGRILNINVLTNGDFGYDRGLDLRIDRRMSNWL
                     TAQIGYTFEVANGTGSSPYSYLTTAARSISGVTGNVLPPAEQPRAVNDQRTHNIVGAV
                     SFTVPRGWQDGTTVGSILKDVSAFATFQVLSGLPYTPLTNVGGGATAPGTNFGLTAAQ
                     AGDINSATTPWTKNLDLRINKGLKLGRLDVTAFADIRNVFNWKNITGLFAETNDVTNG
                     VFEHNTIASEFTNLSNEAQRNGALRSDGSVSLSNCASWTGESGPVNCVELQRAEARFG
                     NGDGVYTVAEENKALSSYYNLFFGPYGFNGPGRSMRLGFEVAF"
     gene            complement(7372..10023)
                     /locus_tag="VFU75_03895"
     CDS             complement(7372..10023)
                     /locus_tag="VFU75_03895"
                     /EC_number="5.1.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HEU5466005.1"
                     /translation="MVRTSWVNLNGLWDYAVRDSGAPAPDSYDGAILVPFPIESQLSR
                     VTRAVTPVQRLWYHRRFTLSPQPPGRRWLLHFGAVDWDATVSVDGHRVGRHRGGYDPF
                     SFDITGALKPGSTHELTVNVWDPTDAGEEPRGKQVLKPGSIWYTAVTGIWQTVWLESV
                     PRTHVDDLSITPDVDAGLVRVTAMAGGTATPVRIEVRSGDASVAVASGVTGRAVSIPI
                     PHARLWSPADPFLYHVRITAGGDSVGSYFGMRKIAVARDSSGALRLFLNNLPLFEYGT
                     LDQGWWPDGLYTAPTEDARRNDFVVLKELGFNTMRKHVKVEPERWYYDADSMGVLIWQ
                     DMPSGDIDSDSGRAEYRVELQRMLAALRNHPSIVMWVPFNEGWGQHDTRATAQWVKRM
                     DPSRLVDDASGWTDRGAGDVSDVHSYPGPEIPAADSTRARVLGEFGGLGFPVTGHTWV
                     NRDNWGYVRYPTLDSLWSGYRDLLTKVRALEATGLAAAIYTQTTDVEVEVNGLMTYDR
                     GVIKLPRDAAQAASQLYAPLTGSGSVTKSSFGVLSDGKAVDSYTLANAGGMRVKILTY
                     GGIIQSVEVPDSHGRLGDVVLGFDSLDGYVKQSPYFGAVVGRYANRIARGKFTLDGKE
                     YTLAVNNGPNALHGGLKGFDKVVWSADPFQRGDSVGVLLSYVSPDGEEGYPGTLKVTV
                     RYTLTGANELVVDYDATTDQATPINLSQHSYFNLSAGRSPDVLGHVVTIDAARYTPVD
                     STLIPTGALAPVAGTPFDFRQGTTIGARIGDADQQLKIAGGYDHNFVLHGGPGVTHAA
                     RVVEPATGRTLDVFTDQPGLQFYSGNFLDGSIKGKGGTTYVHRGAFCVETQHFPDSPN
                     HPAFPSTILRPGSTYRTRTVFNFGVTQ"
     gene            10277..>11361
                     /locus_tag="VFU75_03900"
     CDS             10277..>11361
                     /locus_tag="VFU75_03900"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEU5466006.1"
                     /translation="MNPTTLLLLAVSVSPQGPASKPDSPAAPVPTAVAAVRATVPPVL
                     NRFDTDPAWADAPVISEFRVFRPTDGAQPKFRTEAKVIYDARDLYVLVRAYDPHPDSI
                     VGLLARRDVQTPSDMISVFIDSYHDRRTGYEFDVNPVGVKTDYAIYKDGDEDAAWDGI
                     WDVATKVDSLGWVAEFRFPLSQLRFAVGPSNTFGFTIARQVERTGEQDSWPPYHTNKP
                     GLASQLGDLTGLSELAPPRRFELAPYMVTKNVSVAQGGGFGRDQQASIGADFKVAVAS
                     NLTLTGTVNPDFGQVESDPAVLNLGAFETFFQERRPFFVEGNGLFQFNVNCSQVNCSG
                     EELFYSRRIGQTSQLDNGIATTILGAG"
BASE COUNT         1949 a         3774 c         3716 g         1922 t
ORIGIN      
        1 aagtcgttct tccagaagga tgcgccggtg ttcgcgcccg aatggctgcg caccgagcgc
       61 atgtggagcg aggacaccca gcgggagatc gattatttcg tttgcgacga cctggactcc
      121 cttctctaca tcgcgaacct cggcaccatt cccctccacg tctgggggag ccggattgcg
      181 agcctcgagc gccccgactg gtgcatcctc gatctcgacc cgaaagaagc gccgttcacg
      241 gacgtggtga aggtcgcgag ggccacgaag cagctctgcg atcggatcgg gctccccgcc
      301 tacatcaaga cctcgggctc caccggcctc cacgttctca tcccgatggg ccgccaggtg
      361 acctacgaac agtgccgcca gctgggcggc ctcctggccc gggtggtcgc ggcggagttg
      421 ccccacatcg cgacgattac ccgggtggtg gggaagcgcg gagggcgcgt ctacctcgac
      481 tacgggcaga atggccatgg ccgcttgctg gtcgcaccgt tttccatcag acccctgccg
      541 ggcgctccgg tgtcgatgcc cttgaagtgg agtgaggtca cgcccgctct cgagatcggc
      601 cagttcacga tcaagaacgc ccccgcgcgg atgaagaagt tgaagcgcga tcccgtgatc
      661 cctgtactgg acgagcaacc cgacctcgtg aaggcgctcg gactccttca ggcacgggtg
      721 tgaccgccaa gccgctctcg atcggcgtcc ccgagctcgg actcgaagtg tcggggctcc
      781 tggagctccc ggcccatgcg cgcgcgctgt acgtgctggc gcacggcgcg ggggcgggga
      841 tgcgtcatcc gtttctggaa aggatcgcga gcggcctcgc ggccgccggc atgggaacgc
      901 tccggtacca gttcccctac atggagcgcg ggagcggccg acccgatacc cccgacaagt
      961 gcgtggcgac ggtcacagcg gcggtcgcca gcgcgcgagc ggaagcaccc gcgttgccgc
     1021 tcgtggcggg tgggaaatcg ttcggaggcc gcatgacctc gacggcgcag tcccgcgcgc
     1081 ccttggacgg cgtgcgcgga ctcattttcc tgggcttccc gcttcacgct cccggcaagc
     1141 ccgggatcac gcggggcgag cacctggacc tggtgaagat cccgatgctt ttcctgcagg
     1201 ggacccggga tgagttcgcg cagccggagc ttttgcatca ggttgtcgcg cggttgaaac
     1261 tccgggctac gttgcatgaa gtcgaaggcg gggatcattc attcaaggtg ccgaagaagc
     1321 aggcgaccga ggcgagcatc tttgaggatc tcgtcaccac catcaccgac tggaccgacc
     1381 ggagcgccag atgagctact ggattctgaa aaccgaaccg accgcctaca gctacgaccg
     1441 cctggagaag gaaaagaccg cggtctggga tggcgtcgcc aatcccgtcg ccttgaagaa
     1501 cctcaaggcc atggaaaagg gcgacgagct gatgatctat cacaccggcg cggagaagtc
     1561 ggtcatcggc accgcgtcgg tggcgaaaga agcctatccc gatccgaagg ccggcaatcc
     1621 gtcgatggtc gtgatcgaca tcaaggtggg gaagcgcctg accaagcccg tcacgctcgc
     1681 gcagatcaag gccgatgcca cctttgcgga gatggcgctg gtgcgccagg gacggttgtc
     1741 ggtcgtaccg gcgacggcca cgcaatggaa gcgcctcctg gcgctggcgg agtagtgtaa
     1801 cgcggatcac aatgacgccg ccgttggcct ccccggcggc gcgctctacc caaggcccgg
     1861 cgcgcttcgc cgccctccat tctcggcctc ccgtggccgt agtagactaa gccccatggc
     1921 ctttcgcccc gagcccgaac cccgcgcccg cttttcctgg aaggtcgtcc tgaccctctg
     1981 gacgatttac ggcttgacca cgtccctgca ggagctcgtc ggggcgatgg cgtacgggcg
     2041 gcacgagccg tggtggttcg gccccgcgca gatgatgccc caggcctatc tctgggcgtt
     2101 gctcacgccg ttcatcctgt ggtgggggaa ccgcttcccg ccgcgaggac cggggtggcc
     2161 cgggcgggtg ctgattcacg tcgcgacgag catcacgatc gtgacgtgca tggactccgc
     2221 gttcgagttg tacctcccgt acctgtccgc ctgggcccac gtcccgctct cggacttgag
     2281 cctccccctc ggatcccgga tcatccgggt gctggcctgg tggctggtgt tcgatacgat
     2341 gctctactgg gtggtgctct tcatcggctc gatgcgggac gcgggcgcgc gggcgcaagc
     2401 gaaggcgatg cgggaatcac agctcgagac ccagcttgcg caggccgagc tgcattccct
     2461 gaagatgcag ctgcacccgc acttcctctt caacgcgctg cacaccgtgg ggaccctggt
     2521 gcgcacgggc aagtcggctc tggcggtgca ggtggtgggc cggctgggag acctgctccg
     2581 gcggatgttg gacggcgcgg ccacccagga ggtgccgctc cgtgaggagc tcgaattcgc
     2641 gcggaactac ctcgacgtgg aacaagcgcg cttctccgat cgtctgaagg tcacctggac
     2701 ggtggagccg ggggcgcagg atgccgccgt cccgcacctc atcctccagc ccctgctcga
     2761 gaacgccatc cggcacggga tttccgcggc taccgcctca gggatcctga tcgtcggtgc
     2821 ccggtgtacc aatgggacgc tgcacctgac ggtccgggat gatggccccg gaatggcgga
     2881 cgacccgggc cggagcgtgg gatcgagcgg cgtcggattg gcgaacaccc ggctcaggct
     2941 cgcacggctc tacggcgacc gcgccgggct cgaggttctg aacgcggaag acgggggcgt
     3001 cacctctcac gtctttctgc ccttccgccc cgccgccccg gggcgctgac gaatggagag
     3061 cacgatgcgc ggcatgcgaa ccctgatcgt cgacgacgaa cccgccgccc gcgagggtct
     3121 gcggcatctc ttgtccgccg atccggagat cgaggtggtg ggggagtgcc gggacgggaa
     3181 gacggcgacc gccgccattc gcgaccagtc acccgagctc gtctttctcg acgtccagat
     3241 gcccgagctc gacggcctcg gcgtcctgcg cgacgtgccc ccggagcggc gtcccgtcgt
     3301 ggtgttcgtg acgggatacg accgctacgc gctccaggcc ttcgaggtcc acgcggtcga
     3361 gtatctcctc aagcccttca ccgacgaccg gtttcgcgag gcgctgagcc aggccaagct
     3421 ccaggtccgc cggacccgca tgggcgagct gggcaaggag atggcgacgc tcgccggcgg
     3481 cgcaaacggc gcctccgccg tcaccgagaa gtccgcgccg gccgcggggt accgctcgcg
     3541 tctggtcgtg aagacgggag gcaacgtcgt cctggttccg gttgaagaca tcgactggat
     3601 cgacgccgac ggagactacg tccgcattca cgtcggcaag gtgtcgcaca cggtgcgcga
     3661 gaccatgcac cacctcgaga ccgagctgga tccggcgcgc ttcgtccgca tccaccggtc
     3721 caccatcgtg aatctcgcgc gcgtgaagga gctgcagccc ttctaccgcg gcgagttcgt
     3781 cgccgtgctc cacgacggca cccgcctcaa gttgtcccgg ggttgccggg acaccttgga
     3841 ggcccgcctg ggtcgccgtt tgtaacggtt tttcgtcgcc cccctctgac ggttggtcgt
     3901 caggccagcg cggctcatcg cgcgtgcatt tccaagcccc agatacgtct ctagcgtcac
     3961 agcagccgct gccccccggc ataccgccgg gggccggcgt gccctttcag gtgaggaggg
     4021 tcagttgccg tcttcggggt ccctgatggt tcgcacaccg tctttgaaag gagcacacgc
     4081 atgtcgtttt ctgttcgctc gttgttcgcg ttcaccagcg tcctggtgtt gggtggcggg
     4141 gcgttagcgg cgcagacgac cggcaaagtt cagggctcgg ttaccgggcc cgacggcgca
     4201 ccgctcgccc atgcacaggt gacggtgacg ggaacgtcct tcggcgccgt gaccgacgac
     4261 aagggttact acttcatcaa caacatccct gccgggctct accggatgca ggcgcagtac
     4321 atcgggatgg cgccgggaca ggctgagggc gtccgcatcc tggccgggca gacgctcacg
     4381 cagaacttcc cgctgcagaa caaggctctc acggtcaccg ggatcaacgt ggtggccgcg
     4441 gcggatgcgg cggtgccccg tgaccaggtg acttcgaaga ccatcgtctc cggtgagctg
     4501 gtgcagaacc tgccggtcca gaacgtccgg aacgtggtgg cgttgacgcc gggcgtcgtc
     4561 gagtcgggca acggactggg cgtatcgatt cgcggcggcc gccccggtga ggaagccgtc
     4621 tacatcgacg gcgccccggc cattcgtcag gcccagatcg tgggcggcgc gatgggcgct
     4681 tcgccgacga atctggtcca gaccaacgcg gtggaagagg cctcgatcac cacaggcgcc
     4741 atcgccatca accagggcaa tgcgcagtcg ggcgtgatcg ccgtgaccac tcggtccggc
     4801 ggcgagaagc tctcgggagc gttctccgca cagagcgacg gcatgttcgc caacaccatc
     4861 agcaccggct acaaccgttt cgagggctcg gtgggtgggc cggtgccggg attctccaag
     4921 ctccggttct tcctgtcggg cgtgctgcag ggtcagacgt cgccgttccg ctcgtatggc
     4981 gcagccgaag ttccgtccta caccatgaac ggtgtcgaca ccacggtcca cgtggtcggc
     5041 ccgagcggcg cggaaagtgt cacgctcccg aagtgggtgc agtacagcgg ccagtgcgac
     5101 gcggcgcaga acagcggggt ctcgtgccag gggaaccgcc tgcctttcga ctggacgacg
     5161 aacctgaacc tgcagggcaa gatcagctac ttctacggct cgggctcgag catcgcgctg
     5221 tcggcgatcg cggagggcga ccagaaccgc aactacccga accaggtgat cggtgacccg
     5281 gcgctctaca cgggcacgca cacctgggcc cgtgacgtcg tgctgaactg ggaccactcg
     5341 atcttcaagc aggccgatcg cgcgttgtcc ctgaacctca acctgtcgta cgccacgaac
     5401 cagtcgattt cgggcgtgct cgatccggcg acggaagtct cgacgcggaa tccaatgggc
     5461 ggcatggagt tctccacgct caacttcggc gggttcgggc agttcgcggg caatttcttc
     5521 aacaatcccg agcagatcat ccgcaacatc cggtcgaacg acggcgagcg ggtaccgctg
     5581 ctcaaccgga ccgacctgaa cgcggcacag ccgtaccgga tgaatcctta tgccatgcag
     5641 tcgggtgggt tcttcaccga gggcgtggcg gacgctgccc tgttgaacaa cctctatcac
     5701 gagcagcggc tgtacggccg ggcgcaggtc gactggcagg cgaaccgcta ccaccggttc
     5761 aatttcggcg gcgagctcca gcgctccgac gtggcggcgt ggaacggaat tctgaacagc
     5821 cagatctttt cgaatgcgta tcacaccaag ccgaccaacg gcgcggcgtg ggtctccgac
     5881 cgcctcgacc tgggcgacgt cgtgctggag atgggcgtgc gctacgacta cttcaacagc
     5941 aatgcgctgt tctcgaacac cccgggcttc acgttctcga atccgacgtg gaaccagtcg
     6001 tccgcgtcga acgacaccgc atacaccaac tccctggccc ggaccttcac cccgggcgtc
     6061 gggcactcga cgctgagccc gcggctccag gtgtcgttcc cgatcacgga caacaccgac
     6121 ttccggctgt cctactcgca tcaggtgcag acgccggacg tcaacaccat gctccagggc
     6181 atcaacagcg atctgacctt caccaactcg aacgaccggt tcgggcggga cgtcggcttc
     6241 gggaagacga tcctgttcga gttcggcgtg cggcacgcgt tcacgccgga cctggtggtg
     6301 gatctgtcgg gctacaacaa gagcaaggtc gccgacctct cgtaccggat tttcgggtac
     6361 aatgacccga ccaacccggg ccgcatcctg aacatcaacg tcctcaccaa cggggacttc
     6421 gggtacgacc gcggcctcga tctccggatc gaccgccgga tgagcaactg gctcaccgcg
     6481 cagatcgggt acacgttcga ggtggcgaac ggcaccgggt cgtcgccgta ctcctacctt
     6541 accaccgcgg cgcgctcgat ttcgggcgtc acggggaacg tcctgccccc ggccgaacag
     6601 ccgcgggcgg tgaacgacca gcggacgcac aacatcgtgg gcgccgtctc gttcacggtg
     6661 ccgcgtggct ggcaggatgg caccactgtg ggctccatcc tgaaggatgt gagcgccttc
     6721 gcgacgttcc aggtgctttc cggtctgccc tacaccccgc tgaccaacgt cggcggcggc
     6781 gcgacggcac cgggaaccaa cttcgggctc accgcggcgc aggcggggga catcaattcc
     6841 gccaccacgc cgtggaccaa gaatctcgat ctccggatca acaagggtct gaagctgggc
     6901 cgcctggatg tcaccgcctt cgcggacatc cggaacgtgt tcaactggaa gaacatcacc
     6961 gggctgttcg cggagaccaa cgacgtcacg aacggggtct tcgagcacaa cacgatcgcg
     7021 agcgagttca cgaacctgag caacgaggcc cagcggaacg gtgcgctgcg ttccgatggc
     7081 tccgtctcgc tgtcgaactg cgcctcatgg accggtgagt ctggtcccgt gaattgcgtc
     7141 gagctgcagc gcgcggaggc ccggttcggc aacggggacg gcgtgtacac ggtagcggag
     7201 gagaacaagg ctctgtcgtc gtactacaac ctcttcttcg gtccctacgg cttcaacggg
     7261 cccgggcgga gcatgcggct gggcttcgaa gtcgcgttct aaggcaggat agtgcaggcc
     7321 tccggggccg ggggcgacgc ctttcgaggg cgtcgttccc cggccttcgg cttattgggt
     7381 cacgccgaaa ttgaagaccg tccgggtccg atacgtcgag cccggacgca ggatggtgga
     7441 cgggaacgcg ggatgattcg gtgaatctgg gaaatgctgc gtctcgacgc agaaggcccc
     7501 gcgatggacg taggtagtcc cgcctttgcc cttgatggac ccgtccagga aatttccgga
     7561 atagaactgc agtcccggct ggtccgtgaa cacgtcgagg gtccgccccg tcgccggctc
     7621 caccacacgg gcggcgtgcg tcactcccgg gccaccatgc agcacgaagt tgtggtcata
     7681 cccgccggcg atcttgagct gctgatcggc gtccccgatc cgcgccccaa tggttgtccc
     7741 ctgccggaaa tcgaagggtg tgccggccac gggggcgagg gctcccgtgg ggatgagggt
     7801 cgagtcgacc ggcgtgtagc gcgccgcgtc gatggtgacg acgtgtccaa ggacatcggg
     7861 tgagcggccc gccgagagat tgaagtacga gtgctggctc aggttgatgg gcgtcgcctg
     7921 gtccgtcgtg gcgtcatagt ccacgaccag ctcattcgcg ccggtgaggg tatagcgcac
     7981 cgtaaccttc agcgtgccgg gatatccctc ttcgccgtca ggactgacgt aggacaacag
     8041 gacccccacc gaatccccgc gctgaaacgg gtcggcactc cagacgacct tgtcgaagcc
     8101 cttcaggccg ccatgcaggg cattcgggcc gttgttgacc gcgagcgtgt attccttccc
     8161 gtcgagggtg aacttgcccc gcgcgatccg gttggcgtag cggccgacga cggctccgaa
     8221 gtaggggctc tgtttcacgt agccgtccag cgaatcgaag cccagcacca catcacccag
     8281 gcgcccatgg gagtccggca cttcgacgga ctgaatgatc ccgccatacg tcaggatctt
     8341 cacccgcatc ccgccggcgt tcgcaagcgt gtaactgtcg acggccttcc catcggaaag
     8401 cacgccgaac gacgacttgg tcactgagcc actcccggtg agtggtgcgt agagctgcga
     8461 cgcagcttga gcggcgtcac gagggagctt gatgactccc cggtcgtacg tcatgagccc
     8521 gttcacttcg acttcgacgt cggtggtctg ggtgtagatg gccgccgcca gacccgtggc
     8581 ctcgagggca cggaccttcg tcaacagatc ccggtagccg ctccacagcg agtcaagcgt
     8641 ggggtaccgc acgtatcccc agttgtcgcg gttcacccag gtgtgaccgg tcaccgggaa
     8701 gcccagtccg ccgaattcgc cgaggacgcg ggcgcgggtg gaatcggcgg cggggatctc
     8761 gggccccggg taactgtgga cgtccgagac gtcgccggcg ccccggtcgg tccaccccga
     8821 cgcgtcatcc accaggcggc tcggatccat gcgcttcacc cattgggccg tcgcccgcgt
     8881 gtcatgctgt ccccacccct cgttgaaggg cacccacatc acgatgctgg ggtggttgcg
     8941 aagggcggcc aacatccgct gcagctcgac ccggtactcc gcgcgtcctg aatcagaatc
     9001 gatatcgcca ctgggcatgt cctgccagat cagcacgccc atggaatcgg cgtcgtagta
     9061 ccaacgctcg ggctccacct tcacgtgctt ccgcatcgtg ttgaagccaa gctccttgag
     9121 caccacgaag tcgttgcggc gggcgtcttc ggtcggcgcc gtatacagcc cgtcgggcca
     9181 ccatccctgg tccagcgtgc cgtactcgaa gaggggaagg ttattgagga agagccgcag
     9241 cgcaccggag gagtcgcggg ccaccgcgat cttccgcatc ccgaaatagc tcccgaccga
     9301 gtccccaccc gccgtgatcc gaacgtggta gaggaagggg tcggcaggtg accagaggcg
     9361 ggcgtgcgga atcgggatcg acaccgcgcg gccggtgacg ccgctcgcca ccgccacgct
     9421 cgcgtctccc gagcggacct cgatacggac gggcgtcgcg gtcccgccgg ccatcgccgt
     9481 cacccggacc agcccggcat ccacatccgg cgtgatggac aggtcgtcga cgtgcgtgcg
     9541 cgggacggat tcgagccaca ctgtctgcca gatgcccgtg acggccgtgt accagatgct
     9601 cccgggcttc aggacctgct tgcctcgtgg ttcctcgccc gcgtcggtcg ggtcccagac
     9661 gttcaccgtc aactcgtggg tgctacctgg cttcagggcg cccgtgatgt cgaacgagaa
     9721 cggatcgtag ccgccccggt gccggccgac ccgatgcccg tcgacggaca ccgtggcgtc
     9781 ccagtcgacc gcaccgaagt ggagcaacca gcgacggccg ggtggctgag gcgaaagcgt
     9841 gaagcgccgg tgataccaca ggcgctgcac cggtgtgacg gcgcgagtga cgcgagacaa
     9901 ctgcgactcg atcgggaacg gcacgagaat ggcaccgtca tacgaatcgg gcgcgggcgc
     9961 ccctgaatcc cggaccgcgt agtcccacaa gccgttcagg ttgacccagg aggtccggac
    10021 catctgtggc cgcgggtact cagggagaac ccggtccggc gtgacatcct gcgcccaacg
    10081 ggtctccaga tgggacgact gggcccgaag tggtgaggaa gggagggcaa ggagcccgag
    10141 gattgcgagg atgacgtgac ggcgcatttg gctatgaccg gttgctgaca gagaggtagt
    10201 agaacctacg cgcgccgcgg gactcgggaa cccctcgaat gcaacctttc gcaaagtggt
    10261 gccgtctgat actccaatga acccgaccac gctgctcctc ctggccgtca gtgtcagtcc
    10321 ccagggtccc gcatccaagc cggattctcc ggcggcgccc gtcccgacgg ccgtagcagc
    10381 cgttcgcgcg acggtccccc cggtgctcaa tcgcttcgat accgatcctg cctgggccga
    10441 cgcacccgtg atttccgagt tccgggtctt ccgcccgacc gatggtgcgc agccgaagtt
    10501 tcgcaccgag gccaaggtca tctatgacgc gcgggacctg tacgtgctgg tgcgcgcgta
    10561 tgatccgcac cccgacagca ttgtgggctt gctggcccgg cgtgacgtcc agacgccgag
    10621 tgacatgatt tccgtcttca tcgactccta tcatgaccgg cgcaccggct acgaattcga
    10681 tgtgaacccg gtgggcgtga agaccgacta cgccatctat aaggatggcg acgaggatgc
    10741 cgcctgggac ggcatctggg atgtcgccac caaagtcgac tctctcggct gggtcgccga
    10801 attccggttc ccgttgtcgc agctgcgctt cgccgtcggt ccgagcaaca cgttcggctt
    10861 caccatcgca cggcaggtcg aacggaccgg cgagcaggat agctggcccc cctaccacac
    10921 caacaagccc ggcctcgctt cccagctcgg tgatctgacc gggctgagcg aactggcgcc
    10981 tccgcgccgg ttcgagctcg ctccatacat ggtcacgaag aatgtctcgg tagcgcaggg
    11041 aggcggattt ggacgggatc agcaagcgag catcggcgcg gatttcaagg tcgcggtggc
    11101 ctcgaacctg acgctcaccg ggacagtgaa cccggacttc ggtcaggtcg agtcggaccc
    11161 cgccgtcctg aacctcggcg cgttcgaaac gttcttccag gaacgccggc ccttcttcgt
    11221 cgaggggaac ggcctcttcc agttcaacgt gaattgcagc caggtcaact gcagcggcga
    11281 ggagctcttt tattcccgcc gcatcggtca gacgtcgcag ctcgacaacg gcatcgccac
    11341 caccattctc ggcgccggca a
//
LOCUS       DASUBP010000108         3710 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1079422, whole genome shotgun sequence.
ACCESSION   DASUBP010000108 DASUBP010000000
VERSION     DASUBP010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1079422"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1179)
                     /locus_tag="VFU75_03905"
     CDS             complement(<1..1179)
                     /locus_tag="VFU75_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466007.1"
                     /translation="MTTHCLACHRDIAWLIDSGRGYHATVTAQRCAACHPDHAGRDLA
                     LITWPGGDSGRFDHTKTGWPLEGAHAKVRCETCHRPALRIAPVAALSQRRPGTAGWTG
                     LDRRCASCHADPHRGTLSGACTDCHQLTAWKPATGFDHDSTAYELTGRHRDVACDQCH
                     RGLTAAVAKAGAPAVPAVFGPLPHAECSNCHRDPHGGRLGATCARCHSTAGFTSFTTA
                     TFDHERTRYPLRGAHLSVRCDQCHDFRAGATQRDRPFATCGGCHTDPHAGSATLAGKV
                     VDCAGCHTVSGFTPATYTVAQHATTRFALAGRHQTVACASCHIREPDPAKRVALGSAG
                     VRMRPPLQCVGCHVDPHQGHEDRIGACTTCHTLTAFRPSTVDAAAHTGYAFALTGAHR
                     A"
     gene            complement(1344..2144)
                     /locus_tag="VFU75_03910"
     CDS             complement(1344..2144)
                     /locus_tag="VFU75_03910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466008.1"
                     /translation="MWGSPYYALPLEERVRSPLHIWLRPSGYIGQSAGILSLAVMLFL
                     WLYPLRKRWRWLAFTGSLAAWLDVHVVVALVLPLLVAIHAAWRFDGIIGLGVLSMMIV
                     WASGIVGRYLYTRIPRGKAGIELTRDEIAEDRKRLIGKIAARVRLDAAATAATLSQAS
                     ASPRVVGLVAALRQMVRDWGARRRALRHLEATLKAATPRPTRTEIREVLELAEREMTL
                     AQQASLLDATQQLFRLWHVLHRPVALAALVAVIIHVAVVVALGATWFR"
     gene            2472..>3710
                     /locus_tag="VFU75_03915"
     CDS             2472..>3710
                     /locus_tag="VFU75_03915"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HEU5466009.1"
                     /translation="MTSALVYGVFIALATIPVIRQYRRIRRGERRALAADGGDAEIHP
                     GQHPHIDVSRCIGCGACVTACPEGDVLGVVGGKAAIVEPSRCIGHGLCADACPVGAIE
                     IVTAPPGITAGLPVLSSQYETSIPMMFVVGELGGLALIKNAVNQGRAAVDEIAGRLRR
                     RSPGADSAVHDLCVIGAGPGGISASLRALELKLRYVTFEQEELGGTVAKYPRQKLVMT
                     SPVEFPMFGSFKKLTISKEELLRFWEKIHRESGLVIHAGERVEGVERDSSDIFVVRTT
                     KRSVRARAVVLALGRRGTPRKLGVTGEEQPHVMYSLLDAEAYRGIRGLVVGGGDSAVE
                     AALGLAYQKGNRITLSYRRDAFTRLKARNAERIAKAARKGQVNLIFNSNPVRVQRDRV
                     LLDVRGVRREVPADYVWVFAG"
BASE COUNT          625 a         1146 c         1367 g          572 t
ORIGIN      
        1 cgcgcggtgc gcccccgtca gggcgaacgc gtaccccgtg tgggccgcgg cgtcgacggt
       61 ggatggccgg aaggcggtca gcgtgtggca tgtcgtgcaa gcgccgatgc ggtcttcgtg
      121 accctggtgc gggtccacgt gacaacccac gcactgcagc ggaggccgca tccggacacc
      181 cgcggacccc aaggcgacgc gcttggccgg atcgggctcc cggatgtggc agctcgcaca
      241 cgcaacggtc tggtgacgac ccgcgagtgc gaaccgcgtg gtcgcgtgct gcgcaacggt
      301 ataggtcgcc ggggtgaacc cggagaccgt gtggcatccg gcgcaatcca ccaccttccc
      361 ggccagtgtg gcgctcccgg catgcggatc ggtgtggcag ccgccacacg tggcaaaggg
      421 gcggtcccgc tgggtcgcgc cggcccggaa gtcgtggcat tgatcgcagc gcacggagag
      481 atgcgcaccg cggagggggt agcgcgtacg ctcgtggtcg aaggtggccg tcgtgaagga
      541 tgtgaacccg gcggtactgt ggcaccgggc gcaggtcgcg ccgagccggc ctccatgagg
      601 gtcccggtgg cagttggagc attccgcgtg aggcagtggt ccgaagaccg cgggcaccgc
      661 cggggccccg gccttggcca ccgcagcggt gaggccacgg tggcactggt cacaggcgac
      721 gtcgcggtgg cggccggtga gctcataggc ggtgctgtca tgatcgaagc cggtagccgg
      781 cttccacgcg gtcaactggt ggcagtcggt gcaggcgccg gagagggttc ctcggtgggg
      841 gtcggcgtga cacgaggcgc aacggcgatc gagccccgtc cagccggcgg ttccggggcg
      901 gcgttgggag agcgccgcga ccggtgcaat ccgcagcgcg ggtcggtggc aggtctcgca
      961 gcgcactttc gcgtgtgcgc cttcgagggg ccacccggtc ttcgtgtggt cgaaccggcc
     1021 cgagtctcct ccgggccacg tgatcagggc caggtcgcgc cccgcgtgat ccggatgaca
     1081 ggcggcacac cgctgcgccg tcactgtagc atgatatccc cgtccactat cgatgagcca
     1141 cgcaatgtcc cggtgacagg cgagacagtg cgtggtcatc tcttctttcc gcgtgccgtg
     1201 gcattgcgtg caattccgaa ctccctccaa ctgggcgtgg ggttgcgcga gcggtcccgg
     1261 agagatctga ccgctgaccc ggcgagccga aatgcttgac agggtcagag ccatggcgaa
     1321 cacgacgccg gctggcgcac gagtcaccgg aaccacgtcg ccccgagggc gacgacgacg
     1381 gcgacgtgaa tgatgactgc gacgagcgcg gccagggcca ccggccgatg caggacgtgc
     1441 caaagccgga agagttgctg ggtggcatcc agaaggctcg cttgctgggc cagcgtcatt
     1501 tcgcgctcgg ccagctcgag gacctcgcgg atctcggtac gcgtgggccg gggggtggcg
     1561 gctttgagcg ttgcctcgag atgccggagc gcgcgccggc gagcgcccca atcccgcacc
     1621 atctgccgaa gcgcggcgac cagcccgacg actcgcggcg atgcgctggc ctgagagagc
     1681 gtagcggcag tggctgccgc gtcgagacga actcgggcgg cgatcttgcc gatcaaccgc
     1741 ttccggtcct ccgctatctc gtcgcgggtc agctcgatgc cggccttgcc ccgcgggatg
     1801 cgggtataga ggtaccgccc cacgattccc gacgcccaca cgatcatcat cgacagaacg
     1861 cccaggccga tgatcccgtc aaaacgccac gccgcgtgaa tggccaccag gagcggcagg
     1921 accagcgcga cgaccacatg cacgtcgagc caagcggcca gtgaacccgt gaaggcgagc
     1981 caccgccacc gttttctcag tggatagagc cacaagaaca gcatcaccgc gagcgacagg
     2041 atcccggcgc tctgtccgat gtatcccgag ggacgcaacc agatgtgcag gggtgagcgc
     2101 acgcgctctt cgagcggcaa cgcatagtaa ggggagcccc acaccgccac ggctcccaga
     2161 aggcacacga ggagcaggag cccgcggcgg ggtggggagc gaggtgaggt gcgttcgccg
     2221 ctcgatcgac cgccaagact caccgaaggg cgtgtggggg agccggaagg ccggaccgcg
     2281 gcggtagcct gcgtcatgaa atcctcagcg ggtacgactc ctgcgtgaaa ggcaaaagcg
     2341 aggccacttg cctgggtatc tcatttgtcc ggaaactgtg accctgcaag tggttggaat
     2401 acttgaggta caagcgcaac aagggactgc ctgtctcgcc atgaggcagc gtggttcaat
     2461 tcgaggcaag gatgaccagt gctctcgttt acggggtgtt catcgcactc gcgaccatcc
     2521 cggtaattcg gcaataccga cggattcgac gcggggagcg gcgcgcgttg gcggcagatg
     2581 gcggcgacgc cgagatccat ccggggcagc atccccatat cgacgtgtcg cggtgcatcg
     2641 ggtgcggagc gtgtgtcacc gcgtgtccgg aaggggacgt gctgggtgtg gtcggcggca
     2701 aggcggcgat cgtcgagccg tcgaggtgca tcggccacgg gctatgcgcc gatgcgtgcc
     2761 cggtcggcgc gatcgagatc gtcaccgcgc ctcccgggat tacggcgggg cttccggtcc
     2821 tgtcctccca gtacgagacg agcattccga tgatgttcgt ggtcggcgaa ctgggcgggc
     2881 tggcgctcat caagaacgcc gtgaaccagg ggcgcgcggc ggtggatgag atcgcggggc
     2941 ggttgcgtcg ccggtccccc ggcgccgact cggcggtgca cgatctttgt gtcatcggtg
     3001 ccggaccggg gggcatcagc gcatcgctcc gggccctcga gctgaagctc cgctatgtga
     3061 cattcgagca ggaggagctc ggcgggacgg tggcgaagta cccgcggcag aagttggtga
     3121 tgaccagtcc ggtggagttc ccgatgttcg gcagcttcaa gaaactcacc atatccaagg
     3181 aggagctgct ccgattctgg gagaagattc accgggagag cgggctggtg atccatgccg
     3241 gcgaacgagt cgagggcgtc gagcgcgata gttcggacat ctttgtggtg cggacgacga
     3301 agcgctcggt gcgggcccga gccgtcgttc tggccctggg acggcgcggc accccgcgca
     3361 agctgggcgt gacgggcgag gagcagccac acgtgatgta cagcctcctc gacgccgagg
     3421 cctatcgcgg gatccgcggg ttggtggttg ggggagggga cagcgcggtg gaggcggccc
     3481 tcggcctggc ctaccagaaa gggaaccgga tcacgttgtc gtaccgtcga gacgcgttta
     3541 cccgactgaa ggcgaggaat gccgagcgga tcgccaaagc ggcgcggaag ggccaggtga
     3601 atctgatctt caactcgaat cccgttcgcg tgcagcggga ccgggtactg ctggatgtca
     3661 ggggcgtccg tcgcgaggtg ccggccgatt acgtctgggt gtttgcaggg
//
LOCUS       DASUBP010000109         5213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_71137, whole genome shotgun sequence.
ACCESSION   DASUBP010000109 DASUBP010000000
VERSION     DASUBP010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5213
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71137"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..255
                     /locus_tag="VFU75_03920"
     CDS             <1..255
                     /locus_tag="VFU75_03920"
                     /inference="COORDINATES: protein motif:HMM:NF013183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HEU5466010.1"
                     /translation="RDVTPEATRAAVQRAVPGIAEAIRARGAKGFPRAWLSRGIAGTR
                     GKTLIVNLAGSPRAVKDALPVLDELLEHAVTILRGDPAGH"
     gene            289..1797
                     /locus_tag="VFU75_03925"
     CDS             289..1797
                     /locus_tag="VFU75_03925"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5466011.1"
                     /translation="MSDRVLITTGDLEFAVRVNAQLEGAGFETELATSADEVRQALRR
                     REPPPACLVFTGGLHEPSTAQLLSQARDLPVSTLGILETTEPEPKEFALELGLTGYLV
                     KGADLGEVAQTVRRLIERRRLQERTGIIGESAPIQEVLVKIEQMAPVTSTVLIEGESG
                     TGKELVARAIHDLSPRRGKPFIAVNCAALPETLLESELFGHEKGAFTGAAERRLGRFE
                     LADGGTIFLDEVGEMPAATQVKLLRVLEDRTFFRVGGTQPIRVDVRVVAATNKALRDL
                     VSLGTFRDDLYYRLNVLAIYLPPLRQRRTDIPILVRRFIGEFAKAHDRAFQGITPEAL
                     QHLVDADWPGNVRQLRNLIESMVVLAPAGEIRASDIPADIREGAIRGLPMRVVGPGRE
                     VAGQELQFILRSLVELKLQVEELRRRVDEQPARRVEVIEVGPHGSIPAAEIEGPADTR
                     VVYRAGMSMAEVERAAIEAALKESRGNRRKAAETLGIGERTLYRKLKEYALV"
     gene            complement(1794..4160)
                     /locus_tag="VFU75_03930"
     CDS             complement(1794..4160)
                     /locus_tag="VFU75_03930"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEU5466012.1"
                     /translation="MNRTIAISLLLALSTPPLSGQAVDYLPVFDSLRNLSLRTDSAAS
                     VRDVSLVRDSLTFRLDSGIIYFTGSVGGRAPGAVFVGRGSVSVAVAPAYDVERRELHR
                     VLHDSSTTWPITAAAFLALDSTVAELRARAVAWHAGQDGAAGRVLDHLLSHLFDDRSK
                     LARDGDFLSGVLNADTTGFLLARVVRAGGEDLTLRYDVRAQDGITVLHDAHENAEWPV
                     ASFPITRALSDSEPDMVSDPDVRLGRYTIDAAVDPMHDFSSATVLRFTTPRIRTRWVE
                     FELAEMLHADSLTEEGRPVPFFQAPHNGNLWIRLLDGRQPGDTIAVRLVDHGKLIDTY
                     SLLDVWRRDMPRIAPEISPDKWFQVRDCAEWYPRYGSWQVTDMDLTYRVPRNYQFSSI
                     GRLTDSTRSGDTVITHWQTERPTAWACFNVGRMEERHIADARIPPVVVQMNTEAHKSL
                     DQFLEALTDSGGAVSNLRLLTRANALEDVSGDVTNSLSFFAQRFGAPLYSRYYATEVP
                     VDYGEAFPGMIYLSNYTYIGQRNTGAEEIFRSHEMAHQWWGIGVMPASERDVWLAEGF
                     AEFSALWYMQHIMRDTSKFYDQIGKWRDELVSRGSDVAPLGIGFRVGNEDHPKDYDLI
                     VYEKGAWVLQMLRNLMLNFGDFNEDGFTVTMRDFYTRYRGRKASIRDFQRVVEEHTQE
                     SMGWFFDEWVYGSAIPTYTFSWQVDTAVNGKIPLHLRVRQTDVPDGFFMPVPVELQFA
                     GGRHAYIHLNVRGPLTEGTISLPEAPTAVYFNPLASVLAKVKTERWKR"
     gene            4384..>5213
                     /locus_tag="VFU75_03935"
     CDS             4384..>5213
                     /locus_tag="VFU75_03935"
                     /inference="COORDINATES: protein motif:HMM:NF014904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HEU5466013.1"
                     /translation="MRVCPADAVAVDAERVRIVDEACTRCGLCLPACPHGAIVALGDY
                     ERAVELAAGGKATLILSVESAAYFHPNTPEQVVNACYQAGFRTVHRGVLGDELVAREY
                     LRLFAEPGWGTLIRSTCPVVVEQIRTEHPELVPYLAPVDTPIAAEAKYLKAMYGNDLK
                     VIYAGVCLTEGGSAVDAAITFDDLARLLEARGIKVEDQLPHFERLPEERRRHWSTAGG
                     LPLELLAEQTQASRRFRKVRGLAQLDAMARAVTVDRIDLGFVDILPCDGCLDHPLLGS
                     "
BASE COUNT          884 a         1688 c         1770 g          871 t
ORIGIN      
        1 cgggatgtga cgccggaggc gacgcgggcc gcggtccagc gggccgtgcc cgggatcgcc
       61 gaggcgatca gggcccgcgg tgcgaagggg ttcccccgcg cctggctctc ccgcggaatc
      121 gcaggcacgc gcggcaagac gctcatcgtg aacctcgcgg gatctccccg agcggtgaag
      181 gacgctttgc cggtattgga cgagcttctg gagcacgccg tgacgattct tcgcggtgac
      241 ccagccggtc attgaccctg acacctgacc cctggcacct gacccctgat gtctgatcgc
      301 gtattgatca ccaccggcga cctcgagttc gccgtccgcg tcaacgccca gcttgagggc
      361 gccggcttcg agaccgagct ggcgacctcc gccgacgaag tccgccaggc cctgcgccgg
      421 cgtgagcccc cacccgcgtg cctcgtgttc accggcgggt tgcacgagcc ctcgacggcc
      481 cagctcctct cgcaggcgcg tgacctcccc gtgtccacgc tggggattct cgagaccacc
      541 gagcccgagc ccaaggaatt cgccctcgag ctgggcctga cgggttatct cgtgaagggt
      601 gccgacctgg gggaggtggc gcagaccgtg cgccgcctga tcgaacggcg ccgcctgcag
      661 gagcgcaccg gcatcatcgg tgagtcggct cccattcagg aagtgctggt caagatcgag
      721 cagatggcgc cggtgacatc cacggtgctg atcgagggcg aatcgggcac cggaaaagag
      781 ctggtcgccc gcgccattca cgatctcagc cctcgccgtg gaaaaccgtt catcgcggtg
      841 aattgcgcgg cgcttcccga aaccctcctc gagagcgagc tcttcggcca cgaaaagggc
      901 gccttcacgg gggccgccga gcggcgcctg ggccggttcg agctggccga cggcgggacc
      961 attttcctgg acgaagtggg cgagatgccc gccgccacgc aggtgaaact gctgcgggtg
     1021 ctcgaggacc ggactttctt ccgggtgggg ggcacccagc ccatccgggt ggatgtccgc
     1081 gtagtggcgg cgaccaacaa ggcactccgc gacctcgtgt ccctcgggac gttccgcgac
     1141 gacctctatt accggctgaa cgtccttgcc atttatctgc cccccctgcg ccagcgccgg
     1201 accgacatcc cgatcctggt ccggcgcttc atcggcgaat tcgccaaggc tcacgaccgg
     1261 gcattccagg gcattacgcc ggaggccctc cagcacctcg tcgatgccga ctggcccggg
     1321 aacgtccggc agctgcggaa tctgatcgag tcgatggtgg tactggcacc cgccggagag
     1381 atccgcgcct ccgacattcc ggccgacatc cgggagggcg ccatccgcgg cctgccgatg
     1441 cgggtggtcg ggccggggcg agaagtggcg ggccaggagc tacagttcat cctgcggagc
     1501 ctggtggagc tgaagctgca ggtcgaggag ctgcgacgcc gggtcgatga gcagcccgcc
     1561 cgtcgcgtgg aggttatcga ggtcggcccg cacggctcga ttcccgccgc ggaaatcgaa
     1621 ggtcccgcgg acacccgcgt cgtctaccgg gcgggcatgt cgatggcgga ggtggagcgc
     1681 gcggccatcg aggcggctct gaaggagagt cgcggcaacc ggcgcaaggc ggcggagacg
     1741 ttggggatcg gtgagcggac gctgtatcgc aagctgaagg agtacgcgct ggtctagcgc
     1801 ttccagcgct cagtcttgac cttggcaagc accgaggcca gcggattgaa gtagaccgcc
     1861 gtaggcgctt cagggagaga gatagttccc tcggtgaggg ggccccggac gttcaggtgg
     1921 atgtaggcgt gccgcccgcc ggcgaactgg agctccaccg gtaccggcat gaagaagcca
     1981 tccggcacgt cggtctgccg gacccgcagg tgaaggggga tcttcccgtt caccgcggtg
     2041 tcgacctgcc atgagaacgt gtacgtgggg atggctgatc cgtacaccca ctcgtcgaag
     2101 aaccagccca tcgattcctg ggtgtgctct tccaccactc gctggaagtc cctgatcgac
     2161 gcctttcggc cccggtagcg cgtatagaaa tcccgcatgg tgacggtgaa gccgtcttcg
     2221 ttgaaatcgc cgaagttcag catgaggttc cggagcatct ggagcaccca ggcgcccttc
     2281 tcgtaaacga tcagatcgta gtctttcggg tggtcctcgt tccccacgcg gaacccgatg
     2341 ccgaggggtg cgacgtccga cccccgcgac accagctcat cccgccattt cccgatctgg
     2401 tcgtagaact tggacgtgtc cctcatgatg tgctgcatgt accagagcgc tgagaactcc
     2461 gcgaagccct cggcgagcca gacgtcccgc tcactggccg gcatgacgcc gatcccccac
     2521 cactggtgcg ccatctcgtg ggaccggaag atctcctcgg cgccggtgtt ccgctggccg
     2581 atgtacgtgt agttcgagag gtagatcatc cccggaaacg cctcgccgta gtcgaccggg
     2641 acctcggtgg cataataccg ggagtacagc ggcgcgccga accgctgcgc gaagaatgac
     2701 aggctgttgg tcacgtcccc gctgacgtcc tcgagcgcgt tggcccgcgt gaggagccgc
     2761 aggttggaga cggctccccc cgagtccgtc agggcctcga ggaactggtc gagtgacttg
     2821 tgcgcctcgg tgttcatctg caccaccacc ggcggaatcc gcgcatccgc gatgtggcgc
     2881 tcctccatgc ggcccacgtt gaagcacgcc cacgcggtcg ggcgctcggt ctgccaatga
     2941 gtgatgacgg tgtccccgga gcgggtagag tcggtaagac ggccgatgct cgagaactgg
     3001 taattacgcg ggacccggta ggtcagatcc atgtcggtca cctgccagga accgtaccgg
     3061 ggataccatt cggcacagtc cctcacctgg aaccacttgt cgggcgagat ctccggtgcg
     3121 atccggggca tgtcgcgccg ccagacgtcc aggagcgaat aggtgtcgat caacttcccg
     3181 tggtctacga gccgcaccgc gatggtgtcg cccggctggc gcccgtcgag gagccggatc
     3241 cagaggttgc cgttgtgggg tgcctggaag aacgggaccg gacgtccctc ctccgtcaat
     3301 gaatccgcgt ggagcatttc cgcgagctca aattccaccc agcgcgtacg aatccgcgga
     3361 gtggtaaagc ggagcaccgt cgccgacgag aagtcgtgca tcgggtccac cgcggcgtcg
     3421 atggtgtacc gcccgagccg tacatccgga tccgacacca tgtccggctc cgagtcggac
     3481 agcgcccgag tgatggggaa cgaggcgacc ggccactcgg cgttctcgtg ggcgtcgtgg
     3541 aggacggtga tcccgtcctg agcgcggacg tcataccgca gggtgagatc ctcgccgccg
     3601 gcgcgcacca cccgggccag gagaaacccg gtggtgtccg cgttcaggac gccggaaaga
     3661 aaatcgccgt cccgtgcgag cttggaccgg tcgtcgaaca gatggctcaa gaggtggtcc
     3721 aggacgcgcc ccgcggcgcc gtcctggccc gcgtgccagg ctacggcacg ggctctcagc
     3781 tccgcgaccg tcgagtccag ggccaggaat gcggcggcgg tgatgggcca cgtcgtggag
     3841 gagtcgtgaa gcacgcggtg cagctcacgg cgctcgacgt cataggccgg cgcaactgcg
     3901 accgacaccg agccgcgccc gacgaagacc gcgcctggcg cgcgcccgcc caccgatccc
     3961 gtgaaataga tgatcccgga gtcgaggcgg aacgtcagcg agtcgcgcac gagcgacacg
     4021 tctcgtacgc tcgcagccga gtcggtgcgc aacgacagat tgcggaggct gtcaaacacc
     4081 ggcaggtagt cgaccgcctg gcccgagagc ggtggggtgg agagagcgag cagcagggaa
     4141 attgcgatgg tgcgattcac gctgctcagt ttacgcacca tctcggagtc tggcgagtac
     4201 tccgctaagt gcttttacgt gaatgctttg aaaaacaggc ggcattgaca gtccccgaac
     4261 ccgagtccat tttggggtgc ccttgaaacc cgaaggccga caggccgctt tcgcccgaga
     4321 ggcccggacc tccgagtgga ttttcgcgtc gttccagagc attgcgtcac gtgtttggct
     4381 tgcgtgcgag tctgccccgc ggatgccgtg gcggtggatg cggagcgcgt ccggatcgtc
     4441 gacgaagcct gtacccgatg cggcctctgt cttcccgcct gcccccacgg ggccatcgtc
     4501 gctcttggcg actacgaaag ggcggtcgag ctcgccgccg ggggcaaggc gacgctcatc
     4561 ctgagcgtcg aatcagcggc ctactttcat cccaacactc ctgagcaggt cgtcaacgcc
     4621 tgctatcagg cgggtttccg caccgtccac cggggcgttc tgggagacga gctggtggcc
     4681 agggagtacc tgaggctgtt tgccgagcct gggtggggca ctctgatccg cagtacctgc
     4741 ccggtggtgg tcgagcagat caggaccgag caccccgagt tggtgcccta tctggcgcct
     4801 gtggatacgc cgatcgccgc cgaggcgaaa tacctcaaag ctatgtatgg caatgactta
     4861 aaggtcatct acgccggcgt ctgccttacg gaaggggggt ctgcggttga tgcggccatc
     4921 accttcgatg acctggccag gcttctcgag gcccggggca ttaaggtaga ggaccaactg
     4981 ccccattttg agcggctgcc ggaggaacgg cggcggcatt ggagcacggc gggggggttg
     5041 cccctcgagc tgttggccga gcagacccaa gcatcgcgcc gattcaggaa ggtgcgcggg
     5101 ctcgctcagc tcgacgcgat ggcgcgggcg gtgacggtgg accggatcga cctgggcttc
     5161 gtggacattc tgccctgcga cggctgtctc gaccacccgc tgctggggtc gaa
//
LOCUS       DASUBP010000110        18085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_875009, whole genome shotgun sequence.
ACCESSION   DASUBP010000110 DASUBP010000000
VERSION     DASUBP010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 18085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18085
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875009"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VFU75_03940"
     CDS             complement(<1..797)
                     /locus_tag="VFU75_03940"
                     /inference="COORDINATES: protein motif:HMM:NF015736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5466014.1"
                     /translation="MRPRRIARGSSVERLVARVDAQRPGAPLDDTVPTGFPSIDRLLG
                     GGVRRTDLIILGGDVGSGKSALALGMALRSAQSGAEVVFFSGEMTEERLMERALAIEG
                     RVTVDELRVARVSDQGRAGIGAAAVRLRGLPLTILPLAVGDFESLAVQLEPPREADLI
                     IIDYLQLVPSPATAGRHTQDEELALTLRRLKELAVNRQAAVFVVGQLPAFTTSRPNPR
                     PTLEDFGTLGAVKQHADLVLTIFREEMYAPSGGVEGATELAVVKNRNG"
     gene            complement(802..1176)
                     /locus_tag="VFU75_03945"
     CDS             complement(802..1176)
                     /locus_tag="VFU75_03945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466015.1"
                     /translation="MMRATSPAEERLVTLVAAAPRGPKRDGLYALWLTLRAAEALLPP
                     APVSPKNHRRRLQALESRLASLALPAPLKRALAAARTHLDPATPSAAAVVLAQLVAPA
                     REVLGPEAADAVAVAVKGARID"
     gene            complement(1173..2159)
                     /locus_tag="VFU75_03950"
     CDS             complement(1173..2159)
                     /locus_tag="VFU75_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HEU5466016.1"
                     /translation="MLSILLVRFSSIGDVILTTPLVRALRRRHPEAELTFVTKRAFVP
                     LVSDNPNLTRVVALEPDEPVRHLARRLKPLAPTHGLDLHGSLRSRALRLLVPGKWAGY
                     RKRKLARGWLISTKLDWYGTHAPVPERYFEAARHLDVQPDGGPPEFTIGPQAAARVGE
                     WIRGEGLEGESLAVLAPGAAHVTKRWPVGHWAALAGRLTSRGMTPVIVGGAADRGLAA
                     QIDGVRSAAGTFSLQETGAFLARARVVVSGDTGVMHMATGVGTPVVALFGPTVGQFGF
                     QPYRARATVLERSLDCRPCSAMGSSRCPMGHHRCLDDITPVEVDRAAEQLVG"
     gene            2217..3800
                     /locus_tag="VFU75_03955"
     CDS             2217..3800
                     /locus_tag="VFU75_03955"
                     /inference="COORDINATES: protein motif:HMM:NF020249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional response regulator/alkaline
                     phosphatase family protein"
                     /protein_id="HEU5466017.1"
                     /translation="MARPKSILWVDDEVEGLAGHRRFLEQQGFAVEQAAHGDDALALL
                     RRQPYSVVLLDEQMPGKRGLELLGEIRSIDPAMPVVMVTQSEDEGTLREAIGIQVDDY
                     LVKPVHPRQILSVVTRLLEGDRIRQQRLARDFVGRFRELEGKRASTLAWREWIEHWVE
                     LARWEVKLAAASEPGLADALHSLQVALRKDFTEFVKRQYTRWLAHPEGDRPPLSVDVG
                     AEFLLPVLKRTGKVLFIVVDCLRLDQWEALRDLVTPMFDIEESHYFGIVPTATPYARN
                     AIFSGLYPMEIAARHPEWWGHASDDESLNAHEAELLTEQLADLLGRPVPVRYEKLFTA
                     ADGAELLKRLPAHLAQEGVTALVFNFIDQLTHGRSESAILYEVARDADALRGLTRTWF
                     ERSPVMAALAEAERRGVPVLLTTDHGSIHCETPATVFAKRDATQNLRYKFGEDLRAED
                     PEAAVIVEDLKSYGLPSMGLGVRMALAAGDRFFVYPTKLREYQARYRGSFLHGGVSPE
                     EMILPVALLTPRARGGGPR"
     gene            3801..5597
                     /locus_tag="VFU75_03960"
     CDS             3801..5597
                     /locus_tag="VFU75_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5466018.1"
                     /translation="MRGGARLYSLLRPYASLFIATMLVTVVASVLDGFSLSMLVPFLR
                     TIFHESTLLPVGGSKAQAIIAWIMGAIISTGSPQQALRSVVLVLLVALVFKNLAEYLA
                     AYWNVVIQENVVRDLRVRLFGHLQTLPLGYFQQTKGGQTIARLINDTDQVKTAVTAAL
                     ASFFQNIVMIIVYLGIMLSISVRLSLVALVLAPVLLVVIRPLINRLRRRSREMVAERG
                     DLTSVAGEMVGSIKLIRAYVAEPYEGRRFLEMANRYRKRVLRAQRFSSLTSPVSEVFG
                     GVVLVCVLWYGTTLVLANTIQAEEMMLFLVMALRLMSPLKSISNYPGVMAGALASAER
                     VYEVLDLPSSERDVPGEEPASFDKTIEYRNVSFAYAGNAGVLHDVSCKVSRGQVLAIV
                     GPSGAGKTTMVDLLPRFYEPTAGAVLMDGTPLTRFTRSSLRGLMGIVSQETILLNDSV
                     FANIAYGRQDFTLEQVRAAARAANADDFISRLPQGYDTMLGERGTRLSGGERQRIAIA
                     RAVLRDPPILILDEATSQLDTESERLVQDAIDRLMKHRTVLVIAHRLATVQHADQIVV
                     LKEGRIVERGTHAELYAADGLYRRLHNLQFRT"
     gene            5658..6488
                     /locus_tag="VFU75_03965"
     CDS             5658..6488
                     /locus_tag="VFU75_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466019.1"
                     /translation="MKLDFPIIPAIKSVLAVCDEVVVNVGKSEDETRDLVTSIGDPRI
                     RILDTVWDFTKKNEMLGMETLRAMQACKGSWGIYIQADEVLHEDGARILKETVAECDG
                     DERVEGLLVKYLHFYGGLDIVAQNRRWYRREVRCLRLGRDIRPYQGAQGFRVGPEYRK
                     IRARPTDAVMFHYGWARPAKAIREKYEASKVIYPWSKERSEMEQKRGYLEWLPLLKKF
                     KGRHPAVAQQWVDERAHDPERIIGPRVFKAEHIRFYISDWIERLTGARVFEFRNYTVV
                     "
     gene            complement(6473..7417)
                     /locus_tag="VFU75_03970"
     CDS             complement(6473..7417)
                     /locus_tag="VFU75_03970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5466020.1"
                     /translation="MHVAVASHHRLPVKGYGGTERIVTALARGLVALGHRVTMIAPPG
                     TAIPGTALVSFTAKAYKDPRFSLASYTPRDADILHAHFPVTAVPAGPPFLQTLYGNLR
                     SGEPVPPRNVFLSANHAGRNGSKAFVYAGLDPAEFQFQSKKEDYDFWIGRLHQAKGYH
                     WAIEGAQRTGRRLILAGGWRPTFSRGIKFVGEVDGDKKSQLLANARCLWMPALWDEPF
                     GLTNIEALFSGTPVLGTRRGALPEILTPEVGALCDTLDEMVEASKTIDDRDPAHCRAH
                     AERYFTHLVMAEEYVRFYKHLQETGSLPDGRATPYTTV"
     gene            7448..8548
                     /locus_tag="VFU75_03975"
     CDS             7448..8548
                     /locus_tag="VFU75_03975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5466021.1"
                     /translation="MPAGPAAPLNILQLTFQGDGAGSTQSIFNLSDHLRRRGHRVILG
                     VRDESLLARMGREAGLAVIPLDFRRLSPLASRLAEVIAAERVHVVNSHATRDRRALTW
                     LRWRGRLRAGFVVTRRTMPRTSPLELFLIGHTADRTIAVSQTVARALTARFHPARPLR
                     VVPNGIDLARVDAAPSAESMDFARKTVGDSGRPVIAIVARRKDQEVAIRALAGVERPV
                     VLVCVGVEEDPGLRDAKAVVPDRHLVHFVPFVPEALAFYHVARIAALPSRIEGLSQAL
                     LEAMALGLPVVASRAGGNPDLVTEDAGMLVPPGDPAAWAAAFTRLLVDEALAARLGKA
                     GRARVRGEFTLERTAERTEAVYREALAAIRIV"
     gene            8552..9304
                     /locus_tag="VFU75_03980"
     CDS             8552..9304
                     /locus_tag="VFU75_03980"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5466022.1"
                     /translation="MPAPVTVVIPTLNEAAQIEEAVRHLGWASEVLVADGGSTDGTQA
                     LARKAGARVLDVPGLTIAGQRNAAIATAHTDWVFALDADERITPALAAELERTARQPA
                     HEAYAVGRHNYYLGRLMRRSGWGDNWAVRFFRKDRRFVERRVHEGLESVADTGRLVEP
                     LEHTPYRDLSHHLKKIDLYATWAAQDLRDRGRRARITDILVRPPFTFWRTYFLQLGIL
                     EGWHGAVLCGLASVSVFLKYTRLWELDHRSDG"
     gene            9297..10472
                     /locus_tag="VFU75_03985"
     CDS             9297..10472
                     /locus_tag="VFU75_03985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5466023.1"
                     /translation="MAKTFLLTDEFPPMHTGVARMMGEIALRYPKGELLVSTGQNRDA
                     VETDSAFPSVIDRLPIPTRSLSSLAGLLFWSRRVATLAREAKPRFAWCDSIRPAAYAA
                     KWTHERLGTRYGVLVHGGDVLKELHKIHHSAFARRTARALLGSASVVVANSQWTKEQA
                     QTVLRELGLDPHAERVRVVPLGTDPTQFRPGLDTREVRARHRLENGKWVVTVARLEPY
                     KGIDTSLKAVAEVRKAGHEVGYLVIGAGKKKDAYKKLAQELGIAEAVRFIGTVPDKEL
                     PALYNVAEVYLGVSRRAEGTRVEGFGIALAEASACGLPVIAGRSGGVAEAVKDGETGF
                     VVDPEDPAAVAQALTRLLSDQLLARRLGQAGRKAVETNFNWDRVVRDLREIESQVSS"
     gene            10514..11638
                     /locus_tag="VFU75_03990"
     CDS             10514..11638
                     /locus_tag="VFU75_03990"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5466024.1"
                     /translation="MGELALRYPPKSLVVSTGSYPDSAASDARFSQTIDRVGIRATRL
                     RTLNGLALWTWRASALARTFRPGFAWCGELKPAGYPARFLSRWHGVPYGVVVHGTELL
                     LLDAKIRRSRFKRWTAGGLLSGAAVIAANSTWTGNLAKAVLELLDRPDLAQRVRVVPL
                     GTEPREFRPGIDPRDVRDRYGLTGGPWILTVARIEWHKGIDTMIKAMPAIRAAHPGAR
                     YAVAGTGPRQPQLEQLARETGVADAVRFLGPVSDADLPALYNAADLYVGASRRHDLLV
                     EGFGISLVEASASGLAVVGGRSGGVPDAVRDGETGILVDPDQPAAVAEGVNRLLSDEP
                     LRKKMGAAGRVAVEKYYNWDRVAKDLIDIDREFRRPDNPV"
     gene            complement(11639..12100)
                     /gene="bcp"
                     /locus_tag="VFU75_03995"
     CDS             complement(11639..12100)
                     /gene="bcp"
                     /locus_tag="VFU75_03995"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HEU5466025.1"
                     /translation="MISVGKSAPAFSLDTDAGETLSLKDLKGRPVVLYFYPKDDTPGC
                     TVEACEFRDAFPRFKGSKAVILGISPDSVKSHQKFKEKFDLPFTLLADEDHAIAEKYG
                     VWQKKSMYGRTYMGIARTTFVIDKSGKVAKIFEKVKPKGHAEEVEQAIDEL"
     gene            complement(12111..13190)
                     /locus_tag="VFU75_04000"
     CDS             complement(12111..13190)
                     /locus_tag="VFU75_04000"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU5466026.1"
                     /translation="MIENTEFTLVLGGGGMKGLAHIGVLQALVERGLRPSHIVGSSVG
                     SLIGAAWAAGRSIPQLRDMALALKRKDVFVVAHADMAFKRMHSPALFRREPLEHLIRS
                     LVGESTFADLDPPVIVNTVDINSGMQVLWGLHGLDDAPLVDAIFGSCALPGWLPPREI
                     NGRYYVDGATSDNLPIGAARALSGRMVLAVDVSASTALREDTQEEGFASVFARATEIA
                     MQTLLELRLREWSTPPLYYIHPRVAHIQMFSFSHLREVVEEGYRATIAALDRPAEWPG
                     PGAVGVFPKRRVVVRVERDRCIGCGQCLVQAPQGMFVLDNDRKAVVTKPEQEWSPADG
                     GFIRHCPTYAISARPAPQAAERAQA"
     gene            13277..14476
                     /locus_tag="VFU75_04005"
     CDS             13277..14476
                     /locus_tag="VFU75_04005"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5466027.1"
                     /translation="MTDVVSHPTRVKAVRESLRTLRATEFPWTADTVYLNNASIGPLP
                     ERTRRYLDQFNAKRTAPHLLPDRDIFAGLAAAREAVARLINAEAAEIALATNTTYGLN
                     VAATSLPIQAGEVVLVSDREFPANMYPWLMLEKRGVTVEKVPTTKDGFPDEARILERL
                     RDPKVRVLAISFVQFSNGFKADLKKLGAAARANGSYFVVDAIQGIGNSALDVKDTPVD
                     IMSCGGQKWLLSPWGSGFVYVRKELIAKLEPAVVSWMAFEGTDDFSHLVEYNPVFRED
                     ARRFEMITLPYQDILGFATSLELLEEVGLRNVAEVTQSLHQPVLKWADEHGVRIASPR
                     DERHRSAILCVAPPKPAEAFHHVKRARVVCALREGAIRLSPHFYNTVEEMEKVVEVLD
                     DVLHDKV"
     gene            complement(14549..15802)
                     /gene="tyrS"
                     /locus_tag="VFU75_04010"
     CDS             complement(14549..15802)
                     /gene="tyrS"
                     /locus_tag="VFU75_04010"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEU5466028.1"
                     /translation="MTLLDDLRWRGFVHDATRELAEHLAGGSRTAYCGFDPTAPSLQV
                     GNLMPIMLLAQLQRAGHRPIALMGGGTGLIGDPSGKRSERPLLSKDQIRANLQRQRDQ
                     MARFLNFDPGPSSALLLDNAKWLVEQKLVDFLRDIGKHFSVNVMLQKESVQARLEGGL
                     SYTEFSYMLLQSYDFLHLYREQHCTLQVGGSDQWGNITAGVDLIRRVEGGEAHALVAP
                     LITTASGAKFGKSEGESIWLDPEMTSPYRFYQFWLNTDDRDVIGYLKTFTFLERREIE
                     ALEEQMKSNPGARAAQIALARSVTEMVHGKDALASVVSASQVLFGDFDPRKAPAGVFG
                     ILAGEVPTADSPSATPTLVEAVVAAGLVKSNGEARRSIEQGGIYLNQQRENDVNRKFT
                     SADWIGGYALLRKGKKDYALLQLKT"
     gene            15876..18014
                     /locus_tag="VFU75_04015"
     CDS             15876..18014
                     /locus_tag="VFU75_04015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEU5466029.1"
                     /translation="MAALLPAAPAWWPRSLTRGRVILIAVVGCFFVAGTAYGSWTRVC
                     AGTRCPSISRLMGPKTPSQSAKVYAADGRLIAELGLERRTVLKLTDIPLFVRQAFIAT
                     EDKRFYKHHGIDYWRILGALKANILTLGYSQGFSTITMQLARNVFTDQISRDKTLTRK
                     LKEARVAVEIERNFPKDTILQMYLNQIDLGSGAHGVEAASQVYFGKSAKDLNIAEAAT
                     LAALPKAPASYNPRLHPDRAVRRRNVVIDLMRDQGFVTPEDAELWKAYPLMLTNNRTN
                     YGDVAPYFVEWIRQTLEAEFGQQLYNGGLRIHTTLDLDMQDAAERAIENQLETIESGV
                     YSNNRFPGKTYRDVLENQKASGENSSGFTTYLQGALVSLDVHSGAILAMVGGRDFDDS
                     KYNRVTQALRQAGSTFKPFVYSAAIRAGHPVTEILDDTPLSQPILQKDSQPWNPKDDD
                     DSAWGAIPMRRALYGSRNLATIKLGMSLGEQTVIGEARRYGLTTPIPNVPSIHIGSAA
                     VRPIEMASAYTSFATLGTRSAAYGIDYVEDSKGNVLRRYAPDRTVVMDSSHTWLMVDM
                     MKDVVRRGTAYSAVWGSGFTIPAAGKTGTTDTYTDAWFIGYTPQIVTAVWVGYDLLQP
                     ILPHNAGGGRIAAPAWTAMMKDVYDRRPAPADWPRPDSLITREVDWSNGFLATPFCPD
                     SLHHWDWFYPGTEPTKLCPVHSGLGTGINP"
BASE COUNT         2957 a         6007 c         6090 g         3031 t
ORIGIN      
        1 ccattccggt tcttcacgac cgccaattct gtcgcaccct ccacaccgcc tgacggggca
       61 tacatctcct cgcgaaagat cgtcagcacc agatcggcgt gctgcttgac cgcaccgagc
      121 gtaccgaaat cttcgagggt cgggcggggg ttggggcggc tcgtggtgaa cgcgggcagc
      181 tgcccgacca cgaacaccgc ggcctgacgg ttcaccgcga gctctttcag acgcctgagc
      241 gtcagtgcca gctcttcatc ctgggtgtgt cggccggccg tcgcgggtga gggcacgagc
      301 tgcaggtagt cgatgatgat caggtcggcc tcgcgcggcg gttcgagctg caccgcgagc
      361 gattcgaagt ctcccacggc gagcggcaga atcgtgagcg gcagcccccg gaggcggacc
      421 gcggccgcac cgatgccggc acggccctga tcgctgactc tggccacccg caactcgtcg
      481 acggtcacgc gcccttcgat cgcgagtgcg cgctccatca gccgttcctc cgtcatttcc
      541 cccgagaaga agacgacctc cgcgcccgac tgcgccgaac gcaaggccat gccgagcgcc
      601 aacgcggact tgcccgaccc gacatcgccg cccaggatga tgagatcggt acggcgcacg
      661 ccgcccccca gcaggcgatc gatggacggg aagccggtgg ggaccgtgtc gtcgagtggg
      721 gccccgggac gctgggcgtc gacccgggcg acgagccgtt cgaccgacga tccgcgggca
      781 atccggcgcg ggcgcatggc ttcaatctat cctcgcgccc ttgaccgcga cggccacggc
      841 gtcggccgcc tcgggaccca agacctcgcg cgccggcgcc acgagctgag cgagcacgac
      901 ggcggccgcg gacggcgtgg cgggatcgag gtgggtccgc gcggcggcga gcgctcgctt
      961 gagcggtgcc ggaagcgcca gtgacgccag gcggctttcg agcgcctgga gccggcgccg
     1021 gtggtttttg ggcgataccg gcgccggggg caacagcgcc tcggcggcac gcagggtcag
     1081 ccacagggcg tacaacccat cccgtttggg ccctcgcggc gccgcggcca ccaatgtgac
     1141 gagacgctct tcggcgggac tggtggcgcg catcatccga cgagctgctc ggcggctcgg
     1201 tccacttcta ccggcgtgat atcgtcgaga cagcggtggt ggcccatcgg gcaccggctc
     1261 gagcccatcg cggagcaggg acggcagtcg agggaacgct cgagcacggt ggcgcgggcg
     1321 cggtacggct ggaatccgaa ctgccccacc gtgggaccga acagcgccac caccggagta
     1381 cccactccgg tggccatgtg catcacaccc gtgtctcccg ataccaccac gcgggcgcgg
     1441 gcgaggaacg cgccggtttc ctgaagcgaa aacgtgcccg ccgcgctccg caccccgtcg
     1501 atctgcgccg ccagtccccg gtccgccgcg ccaccgacga tcaccggggt catgccccgg
     1561 gatgtgagtc tgcccgcgag tgccgcccag tgcccgacgg gccagcgctt ggtgacgtgc
     1621 gccgcgcccg gagccaggac cgcgagcgat tctccctcaa gtccctcgcc gcggatccat
     1681 tctcctactc gcgcggccgc ttgcggtccg atcgtgaact ccggcggacc cccatccggt
     1741 tggacgtcga gatgcctggc cgcttcgaaa tagcgctccg gcaccggtgc gtgggtcccg
     1801 taccagtcga gcttggtcga gatgagccag ccgcgggcga gtttccgctt gcgatacccc
     1861 gcccattttc ccggcacgag caagcgaagc gcgcggctcc gcagggaccc gtggagatcg
     1921 agtccgtggg tcggagcgag cggcttgaga cggcgggcca ggtgccggac cggctcgtcg
     1981 ggctcgaggg ccacgacgcg ggtcaggttg gggttgtcgg acaccagggg tacgaaggcc
     2041 cgcttggtga cgaacgtcag ctcggcctcc gggtgacggc ggcgcagggc gcggacgagc
     2101 ggcgtggtga ggatgacatc gccgatggag ctgaagcgga ccagaagaat ggacaacacg
     2161 gggagattgt accctctcgt tgcccggctc cgccagccgg gctagctttg cccgacatgg
     2221 cgcgccccaa gagcatcctg tgggtcgatg acgaagtcga aggcctcgcc gggcaccgcc
     2281 gcttcctcga acagcaggga ttcgccgtcg agcaggcggc gcacggagac gatgccctcg
     2341 cgctcctgcg ccggcagccg tacagcgtgg tgctcctgga cgagcagatg cccggcaaac
     2401 gcgggctcga gctgctcggc gaaatccgca gcatcgatcc cgccatgccc gtcgtcatgg
     2461 tgacgcagag cgaagatgaa ggcacgcttc gcgaggccat cggcatccag gtcgacgact
     2521 acctcgtcaa gccggtgcac ccccgtcaga tcctctccgt ggtcacgcgg ctcctcgagg
     2581 gcgatcgcat ccggcaacag cggctggctc gcgatttcgt cggacggttc cgggagctcg
     2641 aagggaagcg cgcgtcgaca ctcgcctggc gcgagtggat cgagcactgg gtagagctcg
     2701 cacggtggga ggtcaagctc gccgcggcgt cggagcccgg tctggccgac gcgctgcatt
     2761 cgctgcaggt ggccctccgc aaggacttca cggaattcgt gaagcggcag tacacccggt
     2821 ggctcgcgca tcccgagggg gaccggccgc cgctgtccgt ggacgtcggc gcggaattcc
     2881 tgctcccggt gctcaagcgg acgggcaagg tcctgttcat cgtggtcgac tgtctgcggc
     2941 tcgaccagtg ggaagcgttg cgcgacctcg tgacgccgat gttcgacatc gaggagtcgc
     3001 actacttcgg catcgtgccg acggcgacac cgtacgcgcg caacgccatt ttctccgggc
     3061 tttaccccat ggagatcgcg gcgcgccacc cggagtggtg ggggcacgcg tccgacgacg
     3121 aaagtctgaa cgctcatgaa gcggagctgc tcaccgaaca gctcgccgat ctgctgggcc
     3181 ggcccgtgcc ggtgcggtac gagaagctct tcaccgccgc ggacggcgcg gagctcctga
     3241 aacggcttcc ggcgcacctg gcccaggagg gcgtcaccgc cctcgtcttc aacttcatcg
     3301 atcagctgac ccacggccgc tccgaaagcg ccattctgta cgaggtggcc cgggatgccg
     3361 acgccttgcg cggcctcacc cggacctggt tcgagcggtc accggtgatg gccgccctcg
     3421 cggaagccga gcggcgcggt gtgcccgtgc tgctcacgac cgaccacggc tccattcact
     3481 gcgagacgcc ggccaccgtg ttcgccaaac gggatgcgac ccagaacctc cgctacaagt
     3541 tcggtgagga cttgcgggcc gaggatcccg aagcggccgt catcgtcgag gatctgaagt
     3601 cctatggact cccttcgatg ggtctggggg tgcggatggc gctcgcggcc ggggaccggt
     3661 tcttcgtgta tcccaccaaa ctgcgggaat atcaggcccg atatcgcggt tcgttcctgc
     3721 acggcggcgt gtcccccgaa gagatgatcc ttccggtcgc gctcctcacg ccccgcgctc
     3781 gcgggggagg accgcgctag atgcgcgggg gcgcgcgcct ctactcgctg cttcgcccgt
     3841 atgcctcgct cttcatcgcc acgatgctcg tcaccgtggt ggcgtccgtg ctggacggct
     3901 tctcgctgtc catgctggtg ccgttcctcc gcaccatctt ccatgaatcc acgctgctcc
     3961 cggtgggcgg ctccaaagcg caggcgatca tcgcgtggat catgggcgcg atcatctcca
     4021 ccgggtctcc ccagcaggcc ctgcgcagcg tggtactggt cctcctcgtg gcgttggtct
     4081 tcaagaacct cgcggagtac ctggccgctt actggaatgt ggtgattcag gagaacgtgg
     4141 tccgcgatct ccgggtcagg ctcttcgggc acctccagac cctccccctc ggctacttcc
     4201 aacagaccaa agggggacag accattgcgc ggctgatcaa cgacaccgac caggtgaaga
     4261 cggcggtgac ggccgccctg gcgtcgttct tccagaacat cgtgatgatc atcgtctacc
     4321 tcgggatcat gctgtcgatc tcggtgcgtc tgtcactcgt cgccctggta cttgcgccgg
     4381 tgttgctcgt cgtcattcgc cccttgatca accggctgcg gcggcgttcg cgtgaaatgg
     4441 tcgccgagcg cggggacctc accagcgtcg ccggcgaaat ggtggggtcc atcaagctca
     4501 ttcgggccta tgtcgccgag ccctacgagg ggcggcgctt cctggagatg gcgaaccggt
     4561 accggaagcg ggtgctccgg gcccagcgat tctcgtcact gaccagcccg gtcagcgagg
     4621 tgttcggcgg agtcgttctg gtctgcgtgc tctggtatgg gaccaccctc gtactcgcga
     4681 acacgatcca ggcggaggag atgatgctct tcctggtgat ggcgctccgg ctcatgtccc
     4741 cgctcaagtc gatctcgaat tatcccggcg tcatggcggg ggcgctggcg tccgcggagc
     4801 gggtctacga agtcctcgac ctgccttcca gtgagcgtga cgtcccgggc gaagagccgg
     4861 cgtccttcga caagaccatc gaataccgga acgtgtcgtt cgcgtacgcc gggaacgccg
     4921 gggtgctgca cgacgtcagc tgcaaggtgt cgcgagggca agtcctcgcg atcgtcgggc
     4981 cgtcgggggc ggggaagacc acgatggtgg atctgctccc ccgcttctac gagccgaccg
     5041 cgggcgcggt gctgatggat ggcaccccgc tcaccagatt cacccggtcg tcactgcgcg
     5101 gcctgatggg catcgtctcc caggaaacca ttctcctgaa cgactcggtg ttcgcgaaca
     5161 tcgcctacgg ccggcaggac ttcacgctcg agcaggtgcg cgccgcggcc cgcgccgcga
     5221 acgcggatga cttcatttcg cggctccccc agggctacga caccatgctg ggcgagcggg
     5281 gcacgcggct gtcgggaggc gagcgtcagc ggatcgccat cgcccgcgcg gtgctccggg
     5341 acccgccgat cctcatcctc gacgaggcga ccagccagct cgacaccgag tccgaacgcc
     5401 tggtgcagga cgccatcgac cggttgatga agcaccggac cgtgctcgtc atcgcgcacc
     5461 gcctggcgac ggttcagcat gccgaccaga tcgtggtcct gaaggaaggg cgcatcgtgg
     5521 agcgaggcac ccacgccgag ctgtacgcgg ctgacggact ctatcgtcga ctccataacc
     5581 tgcagttcag aacctgaccc ctgacccctg acccccgacc cctgatgctt tctggattca
     5641 cgatcgtacg caatgcggtg aagctggact tccccatcat ccccgccatc aagtcggttc
     5701 tggcggtctg cgacgaagtc gtggtcaacg tggggaaatc ggaggacgag actcgggacc
     5761 tggtcacgtc catcggcgac ccgcgcatcc ggatcctcga cacggtctgg gatttcacga
     5821 agaagaatga gatgctcggc atggagaccc tccgcgccat gcaggcatgc aagggctcct
     5881 ggggcatcta catccaggcc gacgaagtgc tccatgagga cggggccagg atcttgaagg
     5941 agaccgtcgc cgagtgcgac ggggacgagc gggtggaagg cttgctcgtg aaatacctgc
     6001 atttctacgg gggcctggac atcgtcgcgc agaaccgccg ctggtaccgg cgcgaagtcc
     6061 ggtgcctccg cctgggccgc gacatccgtc cctaccaggg cgctcagggc ttccgcgtgg
     6121 gccccgaata ccggaagatc cgcgcccgcc ccaccgacgc cgtgatgttc cactatggct
     6181 gggcccgtcc agcgaaggcc atccgcgaga agtacgaagc ctccaaggtg atctacccct
     6241 ggagcaagga gcgctcggag atggagcaga agcgcggata cctcgagtgg ctgcccctgc
     6301 tcaagaaatt caaagggcgc catccggcgg tcgctcagca gtgggtggac gagcgcgccc
     6361 acgacccgga gcggatcatc ggaccgaggg tcttcaaggc cgagcacatc cgcttctaca
     6421 tctccgactg gatcgagcgg ctcaccggcg cccgggtgtt cgagttccgg aactacacgg
     6481 tggtgtaggg ggtcgcgcgg ccgtcgggca gtgagccggt ttcctgcagg tgcttgtaga
     6541 accgcacgta ttcttccgcc atcaccaagt gggtgaagta ccgctcggcg tgggcccggc
     6601 agtgggccgg gtcacggtcg tcgatggtct tcgatgcttc gaccatctcg tccagggtgt
     6661 cgcacaaggc acctacctcg ggcgtcagga tttcggggag agccccgcgc ctggtaccca
     6721 gcaccggcgt tcccgaaaag agcgcctcga tgttggtgag accgaacggc tcatcccaga
     6781 gcgcgggcat ccagagacaa cgcgcgttcg cgaggagctg ggatttctta tcgccgtcga
     6841 cttctccgac gaacttgatg ccccgggaga acgtgggccg ccatccaccc gccaggatca
     6901 ggcgccggcc cgtgcgctgt gcgccttcga tggcccagtg atatcccttg gcctggtgga
     6961 gccgtccgat ccagaagtcg tagtcttctt tcttgctctg gaactggaac tccgctgggt
     7021 cgagcccggc atacacgaag gccttgctgc cgttgcggcc ggcgtgattg gcggagagga
     7081 acacgtttcg gggcgggacc ggctcgccgg accgcaggtt gccgtacagc gtctggagaa
     7141 agggaggtcc ggctggtacc gccgtgacgg gaaagtgcgc gtggagaatg tcggcgtcgc
     7201 ggggcgtgta ggacgcgagc gagaacctgg gatccttgta ggctttggcg gtgaaggaga
     7261 cgagcgcggt gccggggatc gccgtaccgg ggggcgcaat catcgtgacc cggtggccga
     7321 gggccacgag gccccgcgcc agggcggtga cgatgcgctc cgtgcctcca taccccttga
     7381 ccggcagccg gtggtgtgag gcgacggcca cgtgcatgga gcgaactcta tcactacctt
     7441 cgtttgcatg ccagcggggc cagcagcgcc actgaacatt ctgcagctca ccttccaggg
     7501 tgacggcgcc ggatccaccc aatccatttt caatctctcc gaccatctgc gccgccgcgg
     7561 tcaccgggtc atcctcggcg tccgggacga atccctgctg gcgcgcatgg ggcgggaggc
     7621 cgggcttgcg gtcatcccgc tcgacttcag gcgcctgagt ccgctcgcga gccgtctcgc
     7681 cgaggtgatc gctgccgagc gggtgcacgt cgtgaacagc cacgcgacgc gcgaccgccg
     7741 cgccctgacc tggttgcgct ggcggggacg cttgcgagcg ggattcgtcg tgactcgccg
     7801 caccatgccc cgcacttcac cgctcgaact cttcctcatc ggacacaccg ccgaccgcac
     7861 catcgccgtc agtcagacgg tggctcgcgc gttgacggcg cgattccatc cggcccggcc
     7921 ccttcgcgtc gttcccaacg gcatcgacct cgcccgcgtc gatgcagcgc cgagcgcgga
     7981 atcaatggat tttgcgcgca agacggtggg agactccggc cgcccggtca tcgcgatcgt
     8041 cgcgagacga aaggaccagg aggtcgcgat ccgggcgctg gccggggtgg agcgcccggt
     8101 ggtgctggta tgcgtgggcg tcgaagagga cccgggtctt cgagacgcga aggcggtggt
     8161 gcccgaccgg catctcgtgc acttcgtccc gttcgtgccg gaagcactgg cgttctatca
     8221 tgtcgcccgg atcgccgcgc tgccgtcgcg gatcgagggg ctgtcccagg cgttgctcga
     8281 ggcgatggcg ctcggactcc cggtcgtcgc ctcccgggct ggcgggaatc ccgatctggt
     8341 gacggaggat gccggaatgc tggtgccccc gggtgatccg gccgcctggg ctgccgcctt
     8401 cacacgcctg cttgtggacg aagcgctggc ggcccgattg gggaaagcgg gacgggcccg
     8461 agtccgcggg gagttcacgc tggagcgaac ggccgaacga acggaagccg tataccgcga
     8521 ggccctggcg gccattagga ttgtctaggg tatgcccgcg ccggtcacgg tagtgatccc
     8581 gacgctgaac gaggccgccc agatcgagga ggccgtcagg cacctcggct gggccagtga
     8641 agtgctggtg gccgatgggg gctccaccga cgggacgcag gcgttggcca ggaaggcggg
     8701 ggcgcgggtg ctcgacgtgc ccggcctcac gatcgccggc caacggaatg cggcgatcgc
     8761 aaccgcccac accgactggg tcttcgcgct ggacgcggac gagcggatca cgccggcgct
     8821 ggcggccgag ctcgagcgga cggcgcgcca gcccgcgcac gaggcctacg cggtaggacg
     8881 ccacaattac tacctgggcc ggttgatgcg gcgcagtggg tggggcgaca actgggccgt
     8941 acgctttttc cggaaggacc ggcggtttgt cgaacgacgg gtgcacgaag gactcgaatc
     9001 tgtggccgat acggggcgcc tggtggagcc cctcgaacac actccgtacc gggatctgag
     9061 ccatcacctc aagaagatcg atctctatgc gacgtgggct gcacaggatc tgcgtgaccg
     9121 gggccggcgc gcccggatca ccgacatcct ggtccggccg ccgttcacgt tctggcgcac
     9181 ctacttcctg cagctcggca tcctcgaagg atggcacggc gccgtcctct gcggcctcgc
     9241 ttccgtgagc gtgttcctga agtacacgcg gctctgggag ctcgaccatc gctccgatgg
     9301 ctaagacctt tctcttgacc gacgaattcc ccccgatgca cacgggggtg gcccgaatga
     9361 tgggggagat cgcgctccgc tatcccaagg gtgagctcct cgtctccacc ggccagaacc
     9421 gggacgcggt ggagaccgac tcggcgtttc cgtccgtgat cgaccggctg ccgatcccca
     9481 cgcgttcgct gtccagcctg gccgggttgc tcttctggtc ccgccgggtc gcgacactcg
     9541 cgcgcgaggc caagccccgg ttcgcgtggt gtgactcgat ccgccccgct gcatacgccg
     9601 ccaagtggac gcacgagcgc ctcggcaccc gctacggcgt gctcgtgcat ggcggcgacg
     9661 tgctgaagga gctgcacaag atccaccact cggcattcgc gcggcgcacc gcccgcgctc
     9721 tcctcggctc ggcgtcggtc gtggtggcga acagtcagtg gaccaaggaa caggcgcaga
     9781 cggtgctgcg ggagctgggc ctcgatcccc acgctgagcg ggtgcgcgtc gtcccgttgg
     9841 gaacggaccc gacgcaattc cgtcccgggc tcgatacgcg cgaggtgagg gcgcggcacc
     9901 ggctggagaa cgggaagtgg gtcgtcaccg tggcgcggct cgagccctac aaggggatcg
     9961 acacgtcact caaggcggtg gccgaagtcc gcaaggcggg ccacgaggtc gggtacctcg
    10021 tgatcggcgc cgggaagaag aaagacgcct acaagaagct ggcccaggag ctcgggatcg
    10081 ccgaggcggt gcgattcatc ggcacggtgc ccgacaagga gctgcccgcg ctgtacaacg
    10141 tggccgaggt ctacctgggc gtgtcacggc gcgccgaggg cacccgggtg gaaggattcg
    10201 gcatcgcctt ggccgaagcg agcgcgtgcg gcctgccagt gatcgcgggg cggagtggcg
    10261 gtgtagcgga ggcggtgaag gacggagaga cgggcttcgt ggtggatccg gaggatccgg
    10321 cggctgtggc gcaggcgctc acccggctcc tgtccgatca gctcctcgcc cggcgtctcg
    10381 gacaggcggg ccggaaggcc gtcgaaacga atttcaactg ggaccgcgtg gtacgtgacc
    10441 ttcgggagat cgaatcacaa gtctcctcct gagcttcaat ttccctccgc atggcggcgg
    10501 catcgccaga atgatggggg agctggccct ccgctatcct ccgaaatctc tcgtcgtctc
    10561 gaccggcagc taccccgaca gcgcggcatc tgacgcccgc ttttcgcaga ccatcgaccg
    10621 ggtcggtatt cgggccaccc gtctgcgcac cctcaatgga ttggcgctct ggacgtggcg
    10681 ggcatcggcc ctggcgagga cgttccggcc cggcttcgcg tggtgcggtg agctcaagcc
    10741 cgcgggatac ccggcccggt tcctctcacg gtggcacggc gtgccgtacg gcgtcgtggt
    10801 ccacgggacg gagctgctct tgctcgacgc gaagatcagg cgctcgcggt tcaagcggtg
    10861 gaccgccggc ggactcctgt cgggcgcggc ggtgatcgcc gcgaacagca cctggaccgg
    10921 gaatctcgcg aaggccgtcc tggagctgct cgaccggccc gacctcgcgc aacgcgtgcg
    10981 cgtcgttccc cttggcacgg agccccgcga attccgtccc ggcatcgatc ctcgcgatgt
    11041 gcgcgaccgc tacgggctca ccggtggtcc ctggatcctc acggtggcac gcatcgagtg
    11101 gcacaagggg atcgacacca tgatcaaggc catgcccgcg attcgcgccg cccatcccgg
    11161 ggcgcgatac gccgtcgccg ggaccggccc gcgccagcca cagctcgaac agttggcccg
    11221 agaaacgggc gtcgcggacg cggtgcggtt cctgggtccc gtatcggatg cggatctgcc
    11281 cgccctctac aatgcggcgg atctctacgt cggcgcgtcg cgccggcacg atttgctggt
    11341 ggaagggttc gggatctcgc tagtcgaggc gtcggcgtcg ggcctggcgg tggtcggcgg
    11401 caggtcgggg ggagtgccgg acgccgtacg cgacggcgag acggggattc tggtcgatcc
    11461 cgaccagccg gccgcggtgg cggagggggt gaaccggctg ttgtcggatg agccgctccg
    11521 gaaaaagatg ggcgcggcgg gtcgggtcgc ggtggagaag tactacaact gggatcgggt
    11581 cgcgaaggac ttgatcgaca tcgaccggga gttccggagg ccggataatc ccgtgtagct
    11641 agagctcgtc gatcgcctgc tcgacttcct cggcatgtcc cttcggtttc accttctcga
    11701 agatcttcgc gaccttgccg ctcttgtcga tcacgaacgt ggtgcgcgcg attcccatgt
    11761 aggtccgtcc gtacatcgac ttcttctgcc acacgccgta cttctcggcg atcgcatgat
    11821 cttcgtccgc gagcaaggtg aacggcaggt cgaacttctc cttgaacttc tgatggctct
    11881 tcaccgaatc gggggaaatg ccgaggatga ctgccttgga gcccttgaag cgggggaacg
    11941 cgtcgcggaa ttcgcaggcc tcgaccgtgc agccgggcgt gtcatccttc ggatagaagt
    12001 agaggaccac ggggcggccc ttcaggtctt tcagggacag cgtttcaccg gcgtcggtgt
    12061 cgagtgaaaa cgcgggcgcg gattttccga ccgagatcat tggcgatcct ctaggcttgc
    12121 gcgcgttccg ccgcctgggg ggcggggcgg gcgctgatgg cgtacgtggg acaatgacgg
    12181 atgaagccac cgtcggcggg ggaccactcc tgctccggtt tggtgacgac cgccttccgg
    12241 tcattgtcca ggacgaacat gccctgcggc gcctgcacga ggcactgccc gcaaccgatg
    12301 cagcgatccc gctccacccg cacgaccact cggcgcttcg ggaacacacc caccgctccg
    12361 gggccgggcc attccgctgg ccggtccagg gcggcgatag tcgcgcggta gccttcctcc
    12421 accacctcgc gaaggtgact gaacgagaac atctggatgt gcgccacgcg ggggtggatg
    12481 taatagagcg gaggcgtgga ccactcccgg agcctgagct cgagcagggt ctgcatcgcg
    12541 atttccgtcg cccgcgcgaa taccgaagcg aacccttctt cctgtgtgtc ttcccgcaat
    12601 gccgtcgagg ccgagacgtc cacagcgagc accatgcggc ccgagagcgc gcgcgccgct
    12661 ccgatcggga gattgtcgga ggtcgcccca tccacgtagt agcgcccgtt gatctcgcgg
    12721 ggcggcagcc agccggggag cgcgcaggac ccgaagatcg catccacgag gggcgcgtca
    12781 tcgagcccgt gcaggcccca gagtacctgc atgccggagt tgatgtccac ggtgttcacg
    12841 atcacgggcg gatcgagatc ggcgaacgtc gattccccca cgagggagcg gatcagatgc
    12901 tcgaggggtt cgcgccggaa cagtgcgggg gagtgcatcc gcttgaacgc catatcggcg
    12961 tgcgcgacca cgaagacgtc cttccgcttc aaggccagcg ccatgtctct cagctggggg
    13021 atgctgcgtc cggcagccca ggcggctccg atcagtgacc cgacactgga cccgacgatg
    13081 tgggacggac ggagaccccg ctccaccagc gcctgaagca cgccgatgtg ggccagtccc
    13141 ttcatgccgc ccccgcccag gacgagggtg aattcggtgt tctctatcac caaagggtcc
    13201 gcttgtttcg agaggtgttc cccgggtaag gtagaagagt ccccagcacg tgcgccacta
    13261 gggcgcgagg ccgttcatga ccgacgtcgt ttctcatccg acccgtgtga aagccgtgcg
    13321 cgaatccctg cgcaccctgc gagctaccga gtttccgtgg accgcggaca cggtctattt
    13381 gaacaatgcc tcgatcggac ctctgccgga gcgcacccgc cgttacctcg accagttcaa
    13441 cgccaagcgc accgcccctc acctgcttcc cgaccgggac atcttcgccg gcctggccgc
    13501 cgcgcgtgag gccgtcgccc ggctgatcaa cgccgaggcg gcagagatcg ccctcgcgac
    13561 caacacgacg tacgggctga acgtcgcggc cacatcgctg ccgatccagg ccggtgaggt
    13621 ggtgcttgtg tcagaccggg aattcccggc caacatgtat ccgtggctga tgctggagaa
    13681 gcggggcgtc acggtcgaga aagtcccgac caccaaggac gggttcccgg acgaggcccg
    13741 gatcctggaa cggctgcgcg atccgaaggt ccgggtgctc gcgatctcgt tcgtgcaatt
    13801 ctcgaacggg ttcaaggccg atctcaagaa gctgggcgcc gcggccaggg cgaacggttc
    13861 ctacttcgtg gtcgacgcga tccaggggat cggaaactcg gctctcgacg tgaaggacac
    13921 gccggtggac atcatgtcct gcggcgggca gaagtggctc ttgtcgccct ggggctccgg
    13981 attcgtctac gtccgcaagg agctgatcgc gaagctcgag ccggcggtgg tcagctggat
    14041 ggcgttcgag ggcaccgacg acttctcgca tctggtggaa tacaatccgg tgttccgcga
    14101 agacgcccgc cggttcgaga tgatcacgct gccctatcag gacatcctgg ggtttgcgac
    14161 ctcgctcgag cttctggagg aagtgggtct gcggaacgtg gccgaggtca cgcagtccct
    14221 gcatcagccg gtgctcaagt gggcggatga gcatggcgtg cggatcgctt caccacgcga
    14281 cgagcgccac cggtccgcca ttctctgcgt cgccccgccg aagcccgccg aggcgttcca
    14341 tcatgtgaag cgggcgcgcg tggtgtgcgc cctgcgggaa ggcgcgatcc ggctttcgcc
    14401 gcatttctac aacaccgtgg aggagatgga gaaggtcgtc gaggtcctgg acgacgtcct
    14461 gcacgacaag gtctagatcc aactctcttc cgtcgcgtct cgttcccacc ctcaagggtg
    14521 ggcccgatgc ggcactacgc ttcaagcgtc aggttttgag ctgcagcagg gcgtagtcct
    14581 tcttgccctt cctcaatagc gcgtatcccc cgatccagtc ggcggacgtg aacttccggt
    14641 tcacgtcgtt ttcccgctgc tggttcaggt agatcccgcc ctgttcgatg gaccgccgcg
    14701 cctcgccgtt cgatttcacg agtccggccg cgaccaccgc ttccacgagc gtcggggtcg
    14761 ccgacggcga gtcggctgtg gggacctcac cggccagaat gccgaagaca ccggccggcg
    14821 ctttccgggg atcgaagtcg ccgaacagca cctgggaggc gctcaccacg ctcgcgagcg
    14881 cgtccttccc atgcaccatc tcggtgacgc tcctcgcgag cgcgatctgc gcggcgcgtg
    14941 ctccggggtt gctcttcatc tgttcttcga gcgcctcgat ctctctccgc tcgaggaagg
    15001 tgaaggtctt gaggtagccg atgacgtcac ggtcgtcggt gttcagccag aactggtaga
    15061 accggtacgg cgacgtcatc tccggatcca gccagatcga ctcgccttcg gattttccga
    15121 acttcgcgcc cgacgccgtg gtgatcagcg gcgcgaccag cgcatgggct tcgccccctt
    15181 cgacgcggcg gatcaggtcg acgcccgcgg tgatgtttcc ccactggtcc gagccgccca
    15241 cctgcagggt gcagtgctgt tcccggtaca gatgcagaaa gtcgtacgac tggagcagca
    15301 tgtagctgaa ttcggtgtat gacagcccgc cttcgaggcg cgcctgaacc gattccttct
    15361 ggagcatcac gttgaccgag aagtgcttcc cgatgtcccg gagaaaatcc acgagcttct
    15421 gctcgaccag ccacttggcg ttgtcgagga gcagggccga agagggaccg ggatcgaaat
    15481 tgaggaaccg cgccatctgg tcgcgctggc gctggagatt cgcgcggatc tggtccttgg
    15541 agagcagggg gcgctcgctg cgcttgcccg aaggatcgcc gatcaggccg gtgccacctc
    15601 ccatcagtgc gatggggcgg tgccccgcgc gctggagctg cgcgaggagc atgatgggca
    15661 tcagattgcc gacttgcagc gacggggcgg tgggatcgaa accgcaatac gcggtgcgcg
    15721 agcccccggc caggtgttcc gccagctctc gggtcgcgtc gtgtacgaat ccgcgccagc
    15781 gaaggtcgtc caggagggtc ataggctcgc gaatcgtaaa ggcgggctcc gacgctggca
    15841 agcgcttccc cccgtcagta ggttgttaaa cgacgatggc cgctcttctt cccgccgctc
    15901 ccgcctggtg gccgcgctca ctgacgcgag gccgagtcat cctgatcgcc gtcgtcgggt
    15961 gttttttcgt cgcgggcacc gcctacggat cctggacccg ggtgtgcgcg ggcacgcgtt
    16021 gtccctcgat ctcccgcctc atgggcccca agactccgtc gcagtcggcc aaggtctacg
    16081 cggccgacgg ccggctgatt gccgagctgg gactcgagcg gcgcaccgtc ctgaaattga
    16141 cggacattcc actatttgtt cgacaggcat tcatcgccac ggaagacaag cgcttctaca
    16201 agcatcacgg catcgactac tggcggatcc tgggcgccct gaaagccaac atccttacgc
    16261 tcggctacag ccagggcttc tcgaccatca cgatgcagct cgcccggaac gtgttcaccg
    16321 atcagatctc ccgggacaag acgctcaccc gcaaactcaa agaggcacgg gtggcggtgg
    16381 agatcgagcg gaatttcccc aaggacacga ttctccagat gtacctcaat cagatcgacc
    16441 tcgggtccgg cgcgcacggt gtggaggcgg cctcccaggt ctatttcggc aagtcggcca
    16501 aggacctgaa catcgccgag gcggcgaccc tggcggcgct ccccaaggcg cccgcctcgt
    16561 acaatccccg cctccatccg gaccgcgcgg tgcggcgccg gaacgtggtg atcgacttga
    16621 tgcgcgatca ggggttcgtc acgcccgagg acgcggagct ctggaaggcg tacccactga
    16681 tgctgacgaa taaccgcacc aactacggcg acgtcgcgcc gtacttcgtg gaatggatcc
    16741 gtcagactct cgaggccgag ttcggccagc agctgtacaa cggggggctc cggattcata
    16801 ccacgctcga cctcgacatg caggacgcag ccgagcgggc catcgaaaac cagctcgaaa
    16861 cgatcgagtc cggggtgtac tcgaacaacc gcttcccggg gaagacgtac cgggatgtgc
    16921 tcgagaatca aaaggcctcc ggggagaact cgagcggatt cacgacctac ctgcagggcg
    16981 cgctcgtctc cctcgacgtg cactccggcg cgattctggc gatggtgggt gggcgggact
    17041 tcgacgactc gaagtacaac cgggtaacgc aggcgctcag gcaggcgggg tccaccttca
    17101 agccgttcgt gtattcggcc gcgatccgcg ccggtcaccc ggtgacggaa atcctggacg
    17161 acacgccgct gtcccagccc atcttgcaga aggacagcca gccctggaat ccgaaggacg
    17221 acgacgattc cgcctggggc gccatcccga tgcgccgcgc gttgtacggg tcgcgaaacc
    17281 tcgccaccat caagctgggc atgtccctcg gtgagcagac ggtgatcggg gaggcccgcc
    17341 ggtatgggct cacgaccccg attccgaacg tcccttccat tcacatcggg tcggccgccg
    17401 tacggcccat cgagatggcc tcagcctata cgtcgttcgc gacgctgggg acgcgatcgg
    17461 cggcgtacgg catcgactac gtggaagact cgaagggcaa cgtgctgcgc cggtacgcgc
    17521 ccgaccgcac ggtggtgatg gacagctcgc acacctggct gatggtggac atgatgaagg
    17581 acgtcgttcg ccgcggcacc gcgtacagcg cggtgtgggg ctcgggattc acgatcccgg
    17641 ctgccggcaa gacgggcacg accgacacgt acacggacgc ctggttcatc gggtacaccc
    17701 ctcagatcgt caccgccgtc tgggtcggat acgatctgtt gcagccgatc ctgccgcaca
    17761 acgccggtgg cggccgcatc gcggcgcccg cgtggacggc gatgatgaag gacgtttatg
    17821 accggcgtcc cgcgcccgcc gactggccgc gccccgactc gctgatcacc cgcgaagtcg
    17881 actggtccaa cgggttcctg gcgaccccat tctgtcctga ttccctgcac cactgggact
    17941 ggttctatcc cggcaccgag cctaccaagc tgtgcccggt ccactcgggg ctcggcaccg
    18001 gcatcaaccc ctgaccatgc tccgcattcg cgcggggcgc gtacaccccg tcacggcgcc
    18061 gcccatcgag gacggcgccg ttctc
//
LOCUS       DASUBP010000111        16571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_997758, whole genome shotgun sequence.
ACCESSION   DASUBP010000111 DASUBP010000000
VERSION     DASUBP010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 16571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16571
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_997758"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..344
                     /locus_tag="VFU75_04020"
     CDS             <1..344
                     /locus_tag="VFU75_04020"
                     /inference="COORDINATES: protein motif:HMM:NF012354.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU5466030.1"
                     /translation="DLAAHSRVWLARPAGYLLGAPEARLLEAIERAGSIKEAAVAAAM
                     SYRTAWARVREMERVLGRPVVRSRAGGPGGGATTLTDDARSLVSLFHDLDRRVTEHAH
                     REFTRVIQPPP"
     gene            complement(356..754)
                     /locus_tag="VFU75_04025"
     CDS             complement(356..754)
                     /locus_tag="VFU75_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466031.1"
                     /translation="MKRIASKLILAAALAVLGCSKGTPTGPQPGTLSVQLQTPNSGFD
                     GAMLFTLSGPTTVTNVRAATGDTLWTNDFSSTTTKVVLTGPVGSGVILRFDVPDVNQY
                     GQYLATINQVASSSDYSLRSLTNYVAFVSK"
     gene            complement(751..3030)
                     /locus_tag="VFU75_04030"
     CDS             complement(751..3030)
                     /locus_tag="VFU75_04030"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HEU5466032.1"
                     /translation="MRRPSASVLLAVVLVFPALASAQSRPSRMTPKWEIPGFDFRPDG
                     AYRKWARQVAATRAALLSGGRMAQLNAPVAGGVPAPSSAAVSATLHIPAVLFQFKGGT
                     LPQPGFDTASYSTVLFGASPPAGNPYTVRTFYEQMSNGLLSMQGNALGYVTLDSGENY
                     YAGTPGSCSGSPYGGTQCNGLFSNTAVQHMQIGLVESLRKVDSIIHPDWSTYDYDATT
                     GVLNLVIFIQPALDGACGPQGAPSNHLWSHRFSLQGGPYTTKTPWPGHAGQFLKISDY
                     TLQSGVGGPQGCFDASSIMPIGTATHETGHAFGLPDLYDTQGLSEGVGEWSLMGSGNY
                     TEPYSPSRMDVWSLSQLGWVTVVPLTGGGDYSFGAAPTSDTAFLIRPRGSNPRGEYFL
                     LENRQGVEADSAMIRLHCLVSNAPPTCGGGMLIWHIDSMLVAQDGSIHSCNCVNVGGP
                     GTIHGIAVMQADGAGSLDVNPSSPGSNRGDAGDPFPGVTNNHTYGPPVAVMNSSGSFV
                     GFTVDSITQVTPGGQMSFALRFASLTTITSNDPVGAVIRLDGVPHNRIQVLLDSGSTH
                     VIAVADTQVRSDSLVRYIFQSWSDGGALSHSIVVTGNTDSISAQLALGFRTKVVVDTN
                     GSVTTDRDSALTGAYMIPGTTVNLAEHSHPGSFFLGWAGDTNTTDSTLLLTLTKPYSL
                     HAQFSAPLLLAAVQQQLLQGFSPLTPAQRNWLDQIGNKNGVLDDVGDFLAWVRLTHAV
                     PSSPPAPAPRTMAVKGGRP"
     gene            complement(3054..4451)
                     /locus_tag="VFU75_04035"
     CDS             complement(3054..4451)
                     /locus_tag="VFU75_04035"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU5466033.1"
                     /translation="MSEPQALPRVMRLRDVVLFNVTAIVGLRWLTTASQFGPSSLLLW
                     VLAMLIFFLPSARAVRELTDLDPRSGGMYRWVCRAFGQTHGFVAGWGYWVNNLFYFPS
                     LLVSTAAIAAYAAGPRGVHWADNSWFVGSLALGGLWLAIWLNIVGLRIGKWLQNVGGY
                     TTYVPALFFVAFALWALVARGSSTTFTVHDLLPARFDFTLVNFFATMTFAFAGLELAP
                     SLGEEIQDPVATLRRGVVVSGFVIVAVYLLGTLAMLVALPAATISVTNGMPQAVAALV
                     DRVGVPALAPAAAIVAILLAVSNIGGVGAWLAGSARLPYAAGVDRALPAAFGRVHPKW
                     QTPYVAFLVQGGLASVFIVWGLSGESVRDAYVTLTSTTIILFFIPYLYIFAAYLKLRR
                     DRGLMGLLTGWVGMGAVLLSIGVSLVPPAVERPVAFELKVVGGVIGFMVIGWVLAHQG
                     SVRKPTAAARTAGLP"
     gene            complement(4448..5164)
                     /locus_tag="VFU75_04040"
     CDS             complement(4448..5164)
                     /locus_tag="VFU75_04040"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU5466034.1"
                     /translation="MFRSRGTFLLFAFATSPLVATLAAQGPAPYRVGWYDAVSIGAGG
                     VLAALPIALHLPSGSAPCAPCDPNNVWGIDRVAIHQPDRTADRASTLLLLAAGGAAGL
                     STVWGQDRREAFGNAVVFADAITWAAVADQWVKVTVHRSRPILYTSAAPQAANIRDNR
                     ESFPSGHAELAFAAATAYTVIARRRHLPHATRNAVLLYTAAVIVGTLRVTAARHFPTD
                     VLGGALLGSAVSWAAVTVHP"
     gene            5291..6496
                     /locus_tag="VFU75_04045"
     CDS             5291..6496
                     /locus_tag="VFU75_04045"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HEU5466035.1"
                     /translation="MGSQTLQHAALFEKCKSFTRAREIQAAGLYPYFKPISHSEDTTV
                     VIEGKPRIMMGSNNYLGLTHHPDVLSAAKSALERYGSGCTGSRFLNGTLDLHERLEAE
                     LAEFFGKEACLVFSTGYQANLGLISGLVGRGDIVFLDKLDHASIVDGAKMSQGETIRF
                     NHGDLGGLERKLQRVPEGTGTMVIVDGVYSMEGDIADLPALLKVTQKYGAALAVDDAH
                     SVGVLGPKGDGTAAHHGVVDEVDLIVGTFSKSLASIGGFAAGSESVIHYLRHHARPMI
                     FTAALPPANTAGVLAALHVLQQEPQRRTQLWKNTRHLQDGLRNLGFDIGPTETPIVPV
                     LIGPLEKTFLFWRKLFDAGVFTNPVVPPAVPPSQCRLRTSLMATHTPEQIDKALGIFA
                     ELGKELGVI"
     gene            6493..7608
                     /locus_tag="VFU75_04050"
     CDS             6493..7608
                     /locus_tag="VFU75_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466036.1"
                     /translation="MSPVRVAPVKDGRDFDRFVAFPYDLYKGDPLWVPQLRMDVRTLL
                     SKKKNPFFLHAEAEYFLAWDGDQVVGRIAAIKNDAHNKEHDDKVGFYGFFECVSDQAV
                     ANALFDAAATWVKGKGLDTLRGPMSPSVNDECGLLVDGFDTPPTLMMPHNHRDYVTMT
                     EKAGFTKAKDLLVYESTTTKPPERILRAAEVVAKRKGITLRSFNLKRFKDEVEIIKRL
                     YNDAWEKNWGFVPMTDAEIDHLAKQLKPVVVPDLICFAEREGKVIGFAVALPDMNVAL
                     KKNPSGRLFPGLLKILWAARKISRVRILLLGALAEYRGTGVDALMYHWIWTKGTAKGY
                     NWGEGGWILEDNPAMNNGLLRLGFRVYKTYRIYDRKL"
     gene            7605..8576
                     /locus_tag="VFU75_04055"
     CDS             7605..8576
                     /locus_tag="VFU75_04055"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU5466037.1"
                     /translation="MNAFVTGGTGFVGAHLVQALRARGDRVTCLVRSPAKAQALGWTD
                     VRIVRGDLRDSRALTDGCADADVVYHVAGLIAARNLDEFLAGNREGTRQVQAAAEGAP
                     RRPRLVLVSSQAAGGPNPRGVPATESAPPAPVTDYGRSKLAAEDVVRNGGGPWTIVRP
                     PVVYGERDTETLKLFRLARWGIGAVFGDGRQELSVIYAGDLAQALIAAGTHDAALGRT
                     YYACHPEIHTSRSLVLAIGRAVGKIPFVLPVPAPVARGLLWTIGSAAGLFGRATILTA
                     DKANEFLAPAWTCRADRLTQDTGWRPQTDLATGLARAVAWYTTERWL"
     gene            8573..9490
                     /locus_tag="VFU75_04060"
     CDS             8573..9490
                     /locus_tag="VFU75_04060"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEU5466038.1"
                     /translation="MTGPRSFTKHWLPIDWACACYVGAASVALTRDASGVAGWGWLAV
                     AHVLMAGLICVAPSARARGGSARFWGEWYPMFLLAGLYGEVGILTVNAGFHNDALIQR
                     LEVLAFGSEVSYRWIREMPVVWFSWLVHWCYLSFYAMLFAAPAGLWIVGKHIAARQTI
                     FAITVTFFICYVIFLFFPVAGPRYAFALAHNAATEVTPARIAQWLLDRGDSWGAAFPS
                     SHVAASVVATGMAWRHWRKLGLVLAPFTVGLVLAVVYGQFHYAVDALSGLMLAGVMLA
                     VMQLQKVPVEELPPYLGIEGMAAAPESPT"
     gene            complement(9442..10503)
                     /locus_tag="VFU75_04065"
     CDS             complement(9442..10503)
                     /locus_tag="VFU75_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466039.1"
                     /translation="MNALFALPLWIWMAFAGHDVPPAVAALPMAHVEASRAGELIVDL
                     RGVNLPAHTMHHMILQPPVSRIEVPSGGAVYGFRVEVVDSLGRELPKSLIHHFNVIDP
                     EARELFVPISHRLLAAGSETGEQRVPWLLFGHPVQAGQTLILSAMLHNPTGVSYPDAR
                     VRLILDYTPKGRPWPFFHAYTWQMDVLFPVGDKSFDLPPGRSERWYEGSPAIPGDIVA
                     IGGHLHDYGTDLELKDVTSGKVIWHAKPVTDGAGHVVKIPIGKLYGLLHLGVHIEPSH
                     RYRLTVRYDNPTGQTITNGGMGVVGGLFAPDRHAAWPQVDQHDSLYQADLRQALRLVP
                     GAARQVGDSGAAAMPSMPR"
     gene            complement(10583..11065)
                     /locus_tag="VFU75_04070"
     CDS             complement(10583..11065)
                     /locus_tag="VFU75_04070"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEU5466040.1"
                     /translation="MRLTALAVLVALGAAACGGGDKNANAAPATATPAATPAATTTSA
                     APAGPVVEVKMTGNGTSQASFEPKTLTIAPGTTVRFVNVVGGPHNISFYKDSIPAGAE
                     AVLNAAMANRIDNLQGALLVNANDHYDVSFAGAPDGVYKGFCLPHQALGMKITITVKK
                     "
     gene            11270..12430
                     /locus_tag="VFU75_04075"
     CDS             11270..12430
                     /locus_tag="VFU75_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466041.1"
                     /translation="MRKLHLLALGSLLLAAPAAAQKNWDTEFGIQGGFSRFKLTGVPG
                     SNSMIDVYGIPTSQIIGVFPTTNAVFAVFPLSDKLALEPGLSFMQELGFPGGGPVPNM
                     ATISLRADYAVTRQFYGALGLFSRYVSGLSGTLPNDNPHFQFGLEAAAGFRMHLSPRL
                     NGRVEIQAITVKKQGVAPPFNVYSLLFGLSTPLTEQPAAGNRRGAAPAQHGAWTPQLG
                     VAAGYSSVHVVGGPDLAVFSFPSAGASSYEGLVIAPSAPTIFGIFPLNDRLAAEVGLD
                     AQNLRTGNGTIASFQLAPRADLAFGNRWYGALGPQFHFLKNTSNGKAIVGISGVSVAW
                     GYRFHLSGALNGRIEANYGMNAKRRTGFALGGGPIPPTTAFGVNLGMMMPLK"
     gene            12542..13690
                     /locus_tag="VFU75_04080"
     CDS             12542..13690
                     /locus_tag="VFU75_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466042.1"
                     /translation="MHKVHLLAVGSLLLAAPAVAQQSWDQEFGIQGGYTRLKATGTNS
                     GSARFDSYSIPSATVIGVYPTATAAYAIFPVGDKIAIEPGLSFVQQESQIAIGGSFPT
                     LAGVSLRLDYAITPAIYGALGGYTRYTGGSPGSTVHYSHFQFGLEAAAGYRLHLGPRL
                     NGRIELQAVTVAKASQQIPFNLYSVLFGVSAPLSEPRQSAGARRSQTRTPNAGGWTPE
                     LGVSAGYSNIHLTQGPDFTVLSFPGAGSSSVPGILAVPGAATLFGIFPLADRVALEAG
                     FDWVNFDLASTHLTSFQLAPRADLAFGTRWYAALGPNVHFLRASGGKYEGVTGVTAGW
                     GYRFHLAGALRGRFELSYGVNAKRRTSFGLGIPASVLGLNFGMMLPLK"
     gene            13792..14889
                     /locus_tag="VFU75_04085"
     CDS             13792..14889
                     /locus_tag="VFU75_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466043.1"
                     /translation="MKNRLVAPLAALALLAPLRLPAQQWGRTALGLEGGFTYIKYAGT
                     KFFDSELHLPGFSDGTALPTPPSLYLIVPMSGRLALEPSVTVGLSSNLFFWGTHFQLG
                     LRADYLITSTFYGAVGGVLVRQAGGNGVTNTSGYSVGAQAGLGARFHLTNVIDGRAEA
                     QAQFWGKTRVAVAQNAYSLMLGFSAPLDGTPASRTVGGDARHTHRGIRLGIAGGLSDE
                     HITNGSDQLQLSVPGSGTQAPFVSPPAFFVTIPLSDRLTLEPGFELHRTHYNGFTYAT
                     GALATHLNLALGSGWYGGAGLVEVTKKATRTPIVGLTGLSVQGGYEFKLAGAWDGRLE
                     LSHIILARQRAWHEPPLSVTSLMVGATVPVN"
     gene            14951..16096
                     /locus_tag="VFU75_04090"
     CDS             14951..16096
                     /locus_tag="VFU75_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5466044.1"
                     /translation="MTHPVVPQLAPEDLARRLDSGEPVQVLDVRAPERLTSGRVTLGE
                     KLAFKNLKGSTLYAMPTLDGLGLDTGAPIAVICGHGNSSQRATRFLMEHGVTAFSVTG
                     GMAAWEQVLIPRALPVPSGLSHLIQLDRVGKGALSYVLGSDGEAVVIDAARHLEPYEA
                     IFKAERLTPRAVIDTHAHADYVSGARMAAKRWGVPYFLHEADTRSPYDGTPARMDVNA
                     VGDGDVIAFGNSNLRVEHTPGHTLGSMTLVTAGGPALTGDFIFVRSLGRPDLGGRADE
                     WAPILWRTLERSRSWPADRLILPGHYSSESERAGDRSISRTLDVLRKENSALAIPTER
                     EFLDWVSRTPPPPETYRTIKLANLGLLELSDADIDILEAGANQCAAG"
     gene            16183..>16571
                     /locus_tag="VFU75_04095"
     CDS             16183..>16571
                     /locus_tag="VFU75_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5466045.1"
                     /translation="MPDDLADVYRTTYPALVRFLYRKVWDAERAEDLAQEAFSRALAH
                     RPENPRGWLFVVAANMARDEARKAARERRHLTLLAAEPQDHRPSPEEILDDEARRRRV
                     REALDQLTPRDREVLLLWDAGLSYDEIA"
BASE COUNT         2779 a         5659 c         5399 g         2734 t
ORIGIN      
        1 gcgatctcgc cgcccattcc cgcgtctggc tcgcccgccc cgccggctac ctgctcggcg
       61 ctccggaggc gcgcctcctc gaagctatcg aacgcgccgg atccatcaag gaagcagccg
      121 tcgccgcggc gatgagctac cgcaccgcct gggcccgggt gcgcgaaatg gaacgggtgc
      181 tggggcgtcc cgtcgtgcgc tcgcgcgcgg gcgggcctgg cggcggcgcc accacgctga
      241 ccgacgacgc gcgcagcctc gtctctctct ttcatgatct cgaccggcgc gtcaccgagc
      301 acgcccatcg ggaattcact cgcgtcatcc agcctccccc ctgacggccc ctcgctcact
      361 tcgagacaaa cgccacgtaa ttcgtgagac tccgcagcga gtagtccgac gagctggcca
      421 cctggtttat ggtggcgagg tactggccgt actggttgac gtccgggacg tcgaagcgga
      481 ggatgacgcc gctcccgacg ggcccggtca agaccacctt tgtcgtcgtg ctgctgaaat
      541 cgttggtcca caaggtgtcc ccggtggccg cccgcacgtt ggtcaccgtg gtcggtcccg
      601 acaacgtgaa gagcatcgcg ccgtcgaacc cgctgttcgg agtctgcagc tggaccgaga
      661 gcgtgccggg ctgcggcccc gtcggggtgc ccttcgagca gccgagcacc gcgagggccg
      721 ccgcgagaat gagcttcgac gcaatccgct tcatggccgg cctcccttca ccgccatggt
      781 tcgcggcgcc ggcgccgggg gggatgacgg cacggcgtgt gtcaggcgga cccaggcaag
      841 gaagtcaccc acgtcgtcga gcacgccgtt cttgttgccg atctgatcga gccagttccg
      901 ctgggccggg gtcaggggac tgaagccctg caacagctgc tgctgcaccg cggccagcag
      961 cagcggcgcc gagaactgcg cgtgcaggct gtagggcttg gtgagagtga ggagcagcgt
     1021 gctatccgtc gtgttcgtat cccccgccca gcccaggaag aacgacccgg ggtgggagtg
     1081 ctccgcgagg ttcacggtcg tgccgggaat catgtaggcg ccggtcagcg cgctgtcccg
     1141 atcggtcgtc accgagccgt tggtgtccac cacgaccttg gtccggaagc cgagggcgag
     1201 ctgggccgaa atcgaatcgg tgttgccggt gacgacgatg ctgtgcgaaa gcgccccgcc
     1261 atcggaccac gattggaaga tgtaccgcac cagcgagtcg ctccgcacct gggtgtccgc
     1321 gaccgcgatc acgtgtgtcg acccgctgtc cagtagcacc tgaatgcggt tgtgaggcac
     1381 gccatccagt cgaatcacgg ccccgacggg gtcattggag gtgatcgtcg tcagggacgc
     1441 gaagcgaagt gcgaaagaca tctggccgcc cggcgtcacc tgcgtgatcg agtccaccgt
     1501 gaaccccacg aaggagccac tggagttcat caccgccacc ggcgggccgt acgtgtgatt
     1561 gttcgtgacc ccgggaaacg gatcaccggc gtcgccccgg ttcgagccgg gactgctggg
     1621 gttcacgtcc agggagcccg ctccatccgc ctgcatcacc gcgatgccgt gaatcgtccc
     1681 gggaccgccc acgttcacgc aattgcagct gtgaatggag ccgtcctgcg ccaccagcat
     1741 cgagtcgatg tgccagatca gcatcccgcc accgcacgtc ggcggcgcgt tgctcaccaa
     1801 gcaatgcagg cggatcatcg ctgaatcggc ctcgaccccc tgccggttct cgagcaggaa
     1861 atactcgccg cgcggatttg agccgcgcgg acgaatcagg aaggccgtgt ccgacgtggg
     1921 agcggcaccg aacgagtagt ccccgccgcc ggtgagagga acgacggtca cccagccgag
     1981 ctgactgagg gaccacacat ccatccggct ggggctgtac ggttcggtgt agttcccgct
     2041 ccccatcagg ctccattcac ccacgccttc ggagagcccc tgcgtgtcat agagatcggg
     2101 gagcccgaac gcatgcccgg tttcgtgcgt cgccgtgccg atgggcatga tgctcgaggc
     2161 gtcaaagcac ccctgcggcc cgccgacacc gctttgcagc gtgtagtcgg agatcttcag
     2221 gaactgccca gcatggcccg gccacggcgt cttggtggtg tagggaccac cctgcaacga
     2281 gaatcggtgg gaccacagat ggttggacgg agcgccctgt ggcccgcagg ccccatccag
     2341 cgccggctga atgaagatca ccaggttcag gaccccggtg gtcgcatcat agtcgtaggt
     2401 ggaccagtcg gggtggatga tgctgtcgac cttgcgaagg gattcgacca atccgatctg
     2461 catgtgctgg accgccgtgt tgctgaacaa gccgttgcac tgggtcccgc cgtagggact
     2521 cccgctgcac gatcccggcg tgccggcgta gtagttctca ccggaatcga gggtgacgta
     2581 cccgagcgca ttcccctgca tgctgagcag tccgttcgac atctgctcgt agaacgttcg
     2641 gacggtgtac ggattacccg cggggggaga ggcgccgaac agcactgtgc tgtagctcgc
     2701 ggtgtcgaag ccgggctgtg ggagcgtccc gcccttgaac tggaagagca cggccgggat
     2761 atgcagagtg gcggacacgg ccgccgagct cggcgcggga accccgccgg cgacgggagc
     2821 gttcaactgc gccatccggc cccccgagag cagggcggcg cgagtcgcgg ccacctggcg
     2881 tgcccattta cggtacgcac catccgggcg gaagtcgaat cctggaatct cccatttcgg
     2941 agtcatccgg gacggccggg attgggcgga cgccaaagcc gggaacacga ggaccacggc
     3001 gagcaggacg ctcgcactcg gacgacgcat ctgggaacgc ctcctggaga tccttatggt
     3061 agaccggcag tccgtgcggc ggctgtgggc tttctcacag acccttggtg ggcaaggacc
     3121 cacccgatga ccatgaaccc gatcacgccc cccaccacct tcagctcgaa cgcgacgggg
     3181 cgttcgacgg ccgggggaac gagtgatacc ccgatagaaa ggagaacggc tcccatcccc
     3241 acccacccgg tcaggagccc catcaggccc cggtcccgcc gcagcttcag gtaggccgcg
     3301 aagatatata gataaggaat gaagaagagg atgatggtgg tgctggtcag agttacgtaa
     3361 gcgtcccgaa ccgactcccc cgacagcccc cacacaatga agacgctggc cagccccccc
     3421 tgcaccagaa acgccacgta gggcgtttgc cacttcggat gcacccgccc gaaggcggcc
     3481 ggaagggcgc gatccacacc cgccgcatag gggaggcgcg cggaccccgc cagccaggcc
     3541 ccgaccccgc cgatgttcga caccgcgagg agaatcgcca cgatcgcggc cgcgggggcg
     3601 agcgcaggca cgccgacgcg atccaccagg gcggccaccg cctgcggcat gccgttggtc
     3661 accgaaatgg tggccgccgg cagcgccacc agcatcgcga gcgtcccgag gaggtagacg
     3721 gcgacgatca cgaacccgga gaccaccacg ccccggcgca aggtcgccac cgggtcctga
     3781 atctcttccc cgagcgaagg agccagttcg agtcccgcga acgcgaaggt catcgtcgcg
     3841 aaaaagttga cgagggtgaa gtcaaagcgg gcggggagca gatcgtggac cgtgaaggtc
     3901 gtggagctcc cccgcgccac cagcgcccag agcgcgaacg cgacgaagaa gagggccggc
     3961 acgtacgtcg tatacccgcc gacgttctgg agccacttgc cgatccgcag gcccacgatg
     4021 ttcagccaga tggcgagcca gagtccgccg agggcgaggc ttccgacgaa ccaggagttg
     4081 tcggcccagt gcacgccgcg ggggcccgcc gcgtaggcgg cgatggcggc ggtcgacacg
     4141 aggagtgacg ggaaatagaa aaggttgttg acccagtagc cccaccccgc cacgaacccg
     4201 tgagtctgac cgaaggcgcg gcacacccag cgatacatcc cacccgaccg gggatccaga
     4261 tcggtgagct cgcggaccgc gcgcgccgag ggcaggaaaa agatcaacat cgcgaggacc
     4321 cagagcagca ggctcgaggg tccgaactgg ctcgccgtgg tcagccaccg gaggcccacg
     4381 atcgccgtga cattgaagag cacgacgtcc cggagccgca tgacgcgcgg cagcgcttgt
     4441 ggttcgctca tggatgcacc gtgacggcag cccagctgac cgcggatccc agaagggcgc
     4501 cacccaggac gtcagttggg aaatgccggg ccgccgtgac gcgcagggtg cccacgatga
     4561 cggccgcggt gtacagcaag acggcgttgc gggtggcatg cggaaggtgt cggcggcggg
     4621 cgatcacggt gtacgcggtg gcggccgcga aggccagctc cgcgtggcct gacgggaacg
     4681 attcacggtt gtcgcgaatg ttcgcggcct gcggcgcggc gctggtgtac aagatgggcc
     4741 gactgcgatg gaccgtgact ttcacccact gatcggcgac cgctgcccac gtgatcgcgt
     4801 cggcgaatac tacggcattg ccgaacgctt cacggcggtc ctgcccccag acggtgctca
     4861 ggccggcggc accaccggcg gcgagcagca acagcgtgga agcccgatcg gcggtgcggt
     4921 caggctggtg aatcgccacg cgatcgatgc cccacacgtt gttcggatcg cacggtgcac
     4981 aaggcgccga ccccgaaggg agatgcaagg cgatggggag cgcggcgagc accccgcccg
     5041 cgccgatgga aacggcgtcg taccagccca cgcgataggg ggcgggtccc tgagcggcga
     5101 gcgtcgcgac caggggactc gtcgcgaatg cgaacaggag gaaggtccca cgggagcgaa
     5161 acacgatggg aagaagttac gtatgcgtga cgacgtgtgc taactcgtga cggcgtgtga
     5221 ccttgcgcaa tccccggcgg cgggctaatc tgtagccgcc tttccttttt ctactcactg
     5281 acgaggccct gtgggcagcc aaaccctcca gcacgccgcg ttgttcgaaa agtgcaagtc
     5341 cttcacccgc gcgcgcgaga tccaggccgc cgggctgtac ccctatttca agccgatttc
     5401 ccactccgaa gacaccacgg tggtcatcga ggggaagccg cggatcatga tgggctccaa
     5461 caattacctg gggctcaccc accatccgga cgtgctctcc gccgccaaga gcgcgctgga
     5521 gcgttacgga tcggggtgca cggggtcacg cttcctgaac gggacgctcg acttgcatga
     5581 gcggctcgag gccgagctgg ccgagttctt cggcaaggaa gcctgtctgg tcttttccac
     5641 cggctatcag gcgaatctgg gcttgatctc ggggttggtg ggccggggtg acatcgtctt
     5701 cctcgacaag ctcgatcacg cctccatcgt ggacggcgcc aagatgtcgc agggcgagac
     5761 catccggttc aatcacggcg acctgggcgg cctcgagcgc aagctccagc gggtgcccga
     5821 gggcaccggc accatggtca tcgtcgacgg cgtgtactcg atggaagggg acatcgccga
     5881 cctccccgcc ctgctcaagg tcacgcagaa gtacggcgcg gcactggcgg tggatgacgc
     5941 gcattcggtc ggcgtgctcg gccccaaggg cgacggcacc gcggcccacc acggcgtcgt
     6001 cgacgaggtc gacctcatcg tggggacctt ctcgaagtcc ctggcctcca tcggcggttt
     6061 tgcggctggc tccgagtcgg tgatccacta tctccgccac catgcccggc ccatgatctt
     6121 caccgccgcc ctgccccccg cgaacaccgc cggggtcttg gccgcgttgc acgtcctcca
     6181 gcaggaaccg cagcgccgca cccagttgtg gaagaacacc cgccacctcc aggacgggct
     6241 ccggaacctg ggcttcgaca tcggcccgac ggagaccccg atcgtgccgg tgctcatcgg
     6301 accgctcgag aagaccttcc tcttctggcg gaagctcttc gacgccggcg tcttcaccaa
     6361 ccccgtggtc ccgcccgcgg tgccgccgtc gcaatgccgg ctgcgcacca gcctcatggc
     6421 gacccacacc ccggagcaga tcgacaaggc gctcggcatc ttcgccgagc tcgggaaaga
     6481 gctgggagtc atttgagtcc ggttcgggtc gcgcccgtga aagacgggcg cgacttcgac
     6541 cggttcgtgg catttccgta cgacctctac aagggagacc cgctctgggt cccccagctc
     6601 cggatggacg tccggacgct cctctccaag aagaagaacc ccttctttct ccacgccgag
     6661 gccgagtatt tcctggcctg ggacggggac caggtcgtcg gacgcatcgc cgcgatcaag
     6721 aacgacgcgc acaacaaaga gcacgacgac aaggtcgggt tctacggctt cttcgagtgc
     6781 gtgagcgatc aggcggtcgc caacgccctc ttcgatgccg cggcgacctg ggtgaagggc
     6841 aagggcctcg atacgctccg tggcccgatg agcccctcgg tcaatgacga gtgcggcctc
     6901 ctggtggacg gcttcgatac gccccccaca ctgatgatgc cgcacaacca tcgcgactac
     6961 gtcacgatga cggagaaggc cgggttcacg aaggccaagg acctcctcgt ctacgaaagc
     7021 accaccacca agccgccgga acggatcctc cgcgcggccg aggtcgtcgc caagcgcaag
     7081 ggcatcacgc ttcgcagctt caacctgaag cgcttcaagg acgaggtgga gatcatcaag
     7141 aggctgtaca acgacgcctg ggaaaagaac tggggcttcg tgccgatgac cgacgcggag
     7201 atcgatcatc tggcgaagca gctcaagccc gtggtggtac ccgacctcat ctgtttcgcc
     7261 gagcgcgagg gcaaggtcat cgggttcgcg gtggcgctcc ccgacatgaa cgtggccctg
     7321 aagaagaacc cgtccggcag gctgttcccg ggtctcctga aaatcctctg ggccgcgcga
     7381 aagatctccc gggtccgcat cctgctcctc ggagccctgg ccgagtaccg cgggaccggc
     7441 gtcgacgcgc tcatgtacca ctggatctgg accaagggca ccgccaaggg ctacaactgg
     7501 ggcgagggcg ggtggattct cgaggacaac cccgcgatga acaacggcct cctccgcctc
     7561 ggcttccgcg tttacaagac ctaccggatc tacgatcgca agctgtgaac gcgttcgtga
     7621 cgggggggac cggattcgtc ggtgcccacc tcgtgcaggc gctccgcgcc cgcggcgacc
     7681 gtgtcacctg tctcgtccgc tcccccgcca aagcgcaggc cctcggctgg actgacgtcc
     7741 gcatcgtccg gggtgacttg cgggattccc gggcactcac tgacgggtgc gccgacgccg
     7801 acgtcgtgta tcacgtcgcc ggcctcatcg ccgcccgcaa cctcgatgaa tttctcgcag
     7861 gcaaccgcga ggggaccagg caagtccagg cggcggccga aggagcgccc cggcggccgc
     7921 ggctggtgct ggtatcctcc caagcagccg gcggaccaaa tccgcggggc gtgcctgcga
     7981 ccgaatccgc gccgcccgcg ccggtgaccg actatggccg gagcaagctg gccgccgagg
     8041 acgtggtgcg gaacgggggt ggcccctgga ccatcgtccg tcccccggtc gtgtacggcg
     8101 agcgggacac cgaaacgctg aagctcttcc ggctggcccg gtggggcatc ggcgcggtct
     8161 tcggcgacgg ccgtcaggag ttgtcggtca tttacgcggg agatctggcg caggcgctca
     8221 tcgccgccgg aacccacgac gcggccctcg gccggacgta ttacgcgtgt catccggaga
     8281 tccacaccag ccgttccctg gtgctggcca ttggacgggc cgtcggcaag attcccttcg
     8341 ttctgcccgt gccggcaccg gtggctcggg gccttctgtg gaccatcggg tcggccgccg
     8401 ggctcttcgg ccgggcgacc atactcacgg cggacaaggc caacgaattc ctggctcccg
     8461 cctggacgtg ccgcgccgac cggctcacgc aggacaccgg gtggcgtccg cagacggacc
     8521 tcgccaccgg tctggcgcgg gcggtggcct ggtacacgac ggagcgttgg ctgtgaccgg
     8581 accccggtcg ttcacgaagc actggctgcc catcgactgg gcgtgtgcct gctatgtggg
     8641 cgccgcgtcc gtcgccctca cgcgtgacgc gagcggggtg gccgggtggg gctggctggc
     8701 cgtggcccac gtcctgatgg ccgggttgat ctgcgtagcg ccatcggcgc gcgcccgcgg
     8761 aggcagtgcg cggttctggg gcgagtggta cccgatgttc ctcctcgccg gactctacgg
     8821 ggaagtcggg atcctcacgg tcaacgcggg attccacaac gatgcattga ttcagcggct
     8881 ggaggtgctc gccttcgggt ccgaggtgtc ataccgctgg atccgcgaga tgcccgtggt
     8941 gtggttctcg tggctggtgc attggtgcta tctgtccttc tatgcgatgc tgttcgccgc
     9001 gcccgcgggt ctctggatcg tcgggaaaca catcgcggcc cgtcagacga tcttcgccat
     9061 caccgtgacg ttcttcatct gttacgtgat cttcctgttc ttccctgtcg ccgggccccg
     9121 atacgccttc gctctggcgc acaacgccgc cacggaagtc acccccgccc ggatcgcgca
     9181 atggctcctc gatcgcgggg actcgtgggg cgcggcgttc ccctcgtcgc acgtcgcggc
     9241 ctcggtcgtc gccacgggca tggcgtggcg tcactggcgg aagcttggcc tcgtgctcgc
     9301 gccgtttacg gtggggctcg tcctggccgt ggtctacggg caattccatt acgccgtgga
     9361 tgccttgtcg ggtttgatgc tggcgggcgt gatgctcgcc gtgatgcagc tgcagaaggt
     9421 accggtggag gagctgccgc cttacctggg catcgagggc atggccgccg cccccgaatc
     9481 accgacctga cgcgcggccc ccggcacgag gcggagggcc tgccggaggt ccgcctggta
     9541 cagtgagtcg tgctggtcga cctgaggcca cgcggcatgt cggtccgggg cgaagagccc
     9601 gccgaccacc cccatccccc cattcgtgat ggtctgtccc gtcgggttgt cgtagcgcac
     9661 cgtcaaccgg taacggtgcg acggttcgat gtggacgccg aggtgcaata acccgtacag
     9721 tttgccgatg gggatcttca cgacgtgccc cgccccgtcg gtcacgggct tggcgtgcca
     9781 gatgaccttc cccgacgtca cgtccttcag ctccagatcg gttccgtagt cgtgcaggtg
     9841 cccgccgatg gcgacgatgt ctccgggaat cgccgggctt ccctcgtacc agcgctccga
     9901 acggcctggc ggcaagtcga acgacttgtc cccgacggga aagagcacgt ccatctgcca
     9961 cgtgtacgcg tggaagaagg gccagggacg gcccttgggg gtgtagtcga ggatcagtct
    10021 gacccgcgcg tcagggtagg agacgccggt gggattatgg agcatggcgc tgaggatgag
    10081 cgtctgtccg gcctgtaccg ggtgtccgaa cagaagccac gggactcgct gctcgcccgt
    10141 ctcgctcccc gccgccagca ggcgatggga gatcgggacg aagagctccc gcgcctccgg
    10201 gtcgatcacg ttgaagtggt gaatcaacga cttgggcagc tcgcggccaa gggagtcgac
    10261 gacctccacc cggaagccgt acaccgcccc tccggacggg acttcgatcc gcgacaccgg
    10321 cggctgaagg atcatgtggt gcatggtgtg cgccgggagg ttcacgcccc ggaggtcgac
    10381 gatgagctcg ccggcgcgcg acgcctcgac gtgggccatc ggtagcgcgg cgacggccgg
    10441 cgggacgtca tgacccgcaa aggccatcca gatccacagg ggcagggcga agagggcgtt
    10501 catcgaacct caattataca ccggcaacac accggccccg agggaatccc ccggggccgg
    10561 tgccgttcca gactgcgctg ccttacttct tgaccgtgat cgtgatcttc atgcccagcg
    10621 cctggtgggg caggcagaag cccttgtaga cgccgtcggg agcgccggcg aacgacacgt
    10681 catagtggtc gttggcgttg acgagcagcg cgccctggag gttgtcgatc cggttggcca
    10741 tggcggcatt caacaccgcc tcggcgccgg cggggatcga atccttgtag aacgaaatgt
    10801 tgtgcgggcc accgaccacg ttgacgaacc gaaccgtggt gccgggggcg atggtcaggg
    10861 tcttgggctc gaacgacgcc tggctcgtgc cgttgcccgt catcttcact tcgaccacgg
    10921 gaccggccgg agcggccgag gtggtcgtcg cggcaggggt cgccgcgggg gtcgccgtcg
    10981 cgggagccgc gttggcattc ttgtcaccac caccgcacgc cgcggcgccc agcgccacga
    11041 gtacggccag agcagtcaaa cgcatcgttc gcttcctcca aaggaatacg ggtgcttgtg
    11101 aactcattca caatgtacca aagccccagc gccagcggga gtcctgtcgt ccttcacact
    11161 ccccccgtga catggcgcac agccgaagac cccctccaag cgcatcgtct cgccaccgca
    11221 cgcccggggg ctccggcctt ccggaccact tacttcacgc gagggatgtg tgagaaagct
    11281 ccatctgctg gccttgggta gcctcctcct ggcggctccc gctgctgccc aaaagaactg
    11341 ggacaccgag ttcgggatcc agggtgggtt cagccgtttc aagctgaccg gggtccccgg
    11401 ctcgaacagc atgatcgacg tctacggcat cccgacgtcg caaatcatcg gcgtctttcc
    11461 cactacaaac gccgtgttcg ccgtgttccc cttgagcgac aagctcgccc ttgaaccggg
    11521 tctgagcttc atgcaggagt tgggattccc tgggggtggg cctgtgccca acatggccac
    11581 catcagcttg cgggccgact acgccgtgac caggcagttc tacggcgccc ttgggctgtt
    11641 cagccggtat gtgtcggggc tctccggcac cctcccgaac gacaatcccc acttccagtt
    11701 cggcctggag gccgcggctg gtttccggat gcacctcagt ccccgactga acggccgcgt
    11761 cgagatccag gccatcacgg tcaagaaaca gggtgtcgcg ccgcccttca acgtgtactc
    11821 tctgctcttc ggcctttcga cgcctctcac cgaacagccc gcggcgggca atcggcgggg
    11881 cgccgccccg gcccagcacg gagcctggac gccccagctg ggcgttgcag ccggctactc
    11941 cagcgtgcac gtggtcggtg gtcccgacct cgccgtgttc tcgttcccca gtgccggcgc
    12001 atcgtcctat gaaggcctcg tgatcgctcc gtcggctccg accatcttcg gcatcttccc
    12061 cctgaatgac cgcctggcgg ccgaagtcgg cctggacgcc cagaacctcc gcacgggcaa
    12121 cggcaccatc gcctcgttcc agctcgcccc ccgtgccgac ctggcattcg ggaaccgttg
    12181 gtatggcgcc ctcgggccgc aattccattt cctcaagaac acgtcaaatg ggaaggcgat
    12241 cgtcggcatc agcggggtct cggtggcatg gggctaccgc ttccatttga gcggggccct
    12301 gaacggacgc atcgaggcga actacggcat gaacgccaag cgcagaacgg gctttgcttt
    12361 gggtggcggg ccgatccctc ccaccacggc cttcggcgtg aacctcggga tgatgatgcc
    12421 tctcaagtag cccttacccg accgagtcca cgatgtggca acggctcccg gaaacggggg
    12481 ccgttgcgca tttcggcaca attcgcaaca gttgttccac ttctcaccac gtgagggacg
    12541 tgtgcataag gtccatctgc tggctgtagg cagcctcctc ttggctgccc cggcggttgc
    12601 ccaacaaagc tgggatcagg aattcggcat tcaaggcgga tacacccggc tgaaggcgac
    12661 cgggacgaac tccggcagcg ccagattcga cagctattcc atcccgtcgg ccacggtcat
    12721 tggggtctat ccgacggcca ccgccgcgta cgccatcttc ccggtcggtg acaagatcgc
    12781 catcgaaccc gggttgagct tcgtgcagca ggagagtcag atcgccatcg gcggctcgtt
    12841 ccccaccctc gcgggggtga gcctccgcct ggactacgcc atcacgccgg ccatttatgg
    12901 agccctcggc ggctataccc gctacaccgg tggatcgccg gggagcaccg tacactattc
    12961 ccacttccag ttcgggctcg aggccgcggc cggataccgg ctgcacctcg gtccccgcct
    13021 gaatggccgg atcgagcttc aggccgtcac ggtggcaaag gcgtctcagc agatcccctt
    13081 caacctctat tccgtgctct tcggtgtctc ggctccgctg tcggagccgc gccaatcggc
    13141 tggtgcgcgc cgctcacaaa cgcggactcc gaatgccggt ggctggactc ctgagctcgg
    13201 cgtttcggcg ggctactcga acatccacct cacccaggga ccggacttca cggtgctctc
    13261 gttccccggc gcggggtcat cctcggttcc cggaatcctc gcggtaccgg gcgcagcgac
    13321 gctgttcgga atcttcccgc tggcggaccg ggtggcgctg gaagcgggct tcgactgggt
    13381 gaacttcgat ctcgcgagca ctcacctgac gagcttccag ctggcgccca gggctgatct
    13441 ggccttcggc acccggtggt atgccgcgct tggccccaac gtccacttcc tccgggcgtc
    13501 cggtgggaag tatgaagggg tcaccggcgt cacggctggg tggggctacc gcttccacct
    13561 ggcgggcgcg ctccggggcc gcttcgagct ctcctatggc gtgaatgcca agcggcggac
    13621 cagcttcggc ttgggaatcc cggcttcagt gctgggattg aacttcggca tgatgttgcc
    13681 cctgaagtaa cgaatctcgc ttcatgtatc cgccaacggc ctccgcgtct cgcgggggcc
    13741 gttgcatgtc cgggtgacgc gccggtgacg gcgagcaccg agcatttcgg catgaaaaat
    13801 cgactggtgg cgcccctcgc ggcactcgcc ctgctcgccc cgctccgcct cccggcccaa
    13861 cagtggggcc gaaccgccct cggcctcgaa ggcgggttta cgtacatcaa gtatgcgggc
    13921 acgaagttct tcgactcgga gctgcatctt cccggcttct cggacggcac cgccctcccc
    13981 acacccccat ccctctactt gattgtgccc atgtccgggc ggctcgccct cgagccgtca
    14041 gtcaccgtgg gactttcgag taacctcttc ttctggggca cacacttcca actcggactc
    14101 agagcggatt acctcatcac gtcgaccttc tacggagccg tgggtggcgt cctcgtacgc
    14161 caggcggggg gcaacggggt gaccaatacg tcagggtaca gcgtcggtgc ccaggcgggc
    14221 ctgggcgctc gcttccatct caccaatgtc atcgacggca gggcagaagc gcaggcgcaa
    14281 ttctggggga agaccagggt cgccgtggcg cagaatgcct actccttgat gctcggattc
    14341 tcggcgccgc tcgacggaac gccggcgagc cgcaccgtcg gcggcgatgc ccgccacacg
    14401 caccgaggga tccgtttggg aatcgcggga gggctatccg acgagcacat caccaacggg
    14461 tcggatcaac tgcaactgtc tgttccgggc agcggcactc aagcgccctt cgtctcgcct
    14521 ccggcgttct ttgtaaccat ccctctttca gaccggctca cactcgaacc ggggttcgaa
    14581 ctgcaccgga cccactacaa cggattcacc tatgcgaccg gggccctcgc gacgcacctc
    14641 aacctcgctc tcgggagtgg ctggtatggg ggagcgggtc tcgtcgaggt cacgaagaaa
    14701 gccacccgaa caccgatcgt cggcctcacc ggtttgagcg tgcagggcgg ctacgagttc
    14761 aaactcgccg gcgcctggga cggtcgcctc gagctcagcc atatcatcct ggcccggcag
    14821 cgcgcgtggc atgagccgcc cctctcggtc acctccctga tggtgggagc caccgtcccc
    14881 gtcaactaga gcagccgtag cagatccgga ggcggttgca attcgagccg cctccggcga
    14941 cactatgcgc gtgacccatc cagtcgttcc ccagctcgcg cccgaagatc tcgcccgccg
    15001 cctcgactcg ggcgagccgg tccaggtcct ggacgtgcgc gcccccgagc gcctcacctc
    15061 cggtcgagtg accctggggg aaaagctggc gttcaagaac ctcaagggct cgacgctcta
    15121 cgccatgccc acgctggacg gtctcgggct cgacaccggc gcccccatcg ccgtcatctg
    15181 tggacatggc aactcgagtc agcgtgccac gcgctttctg atggagcatg gtgtcaccgc
    15241 gttctccgtg accggtggca tggcggcctg ggagcaggtc ctcatccccc gcgctctccc
    15301 cgtgccctcg ggactttcgc acctgataca actcgaccgg gtgggcaaag gggcgctgag
    15361 ttacgtcctc ggttcggacg gcgaggccgt cgtcatcgac gccgctcgcc accttgaacc
    15421 gtacgaggcg attttcaagg cggagcgact gacgccccgc gcggtgatcg acacccacgc
    15481 gcacgccgac tacgtcagcg gcgcccggat ggccgcgaag cgctggggtg tcccctactt
    15541 cctgcacgaa gccgacaccc gctcacccta cgacggcacc ccggcccgga tggacgtgaa
    15601 cgccgtcggc gatggcgacg tgatcgcgtt cggcaacagc aacctccggg tagagcacac
    15661 tcccgggcac accctcggca gcatgacgtt ggtcaccgcc ggaggccccg ccctcaccgg
    15721 agacttcatc ttcgtccgct cgctgggccg tcccgacctg ggcggtcggg ccgacgagtg
    15781 ggcaccgatc ctctggcgga ccctcgagcg ctcgcggagc tggcccgccg atcgcctcat
    15841 cctcccgggg cactattcgt ccgaaagcga gcgcgccggc gaccggtcca tctctcggac
    15901 cctggatgtc ctgcgcaagg agaactcggc gctcgccatc cccaccgaac gggaattcct
    15961 ggactgggta tctcgaactc cgcctccccc tgagacttac cgcaccatca aactcgccaa
    16021 cctcggcctc ctggagctct cggacgccga tatcgacatc ctggaggccg gcgccaacca
    16081 gtgcgccgcg gggtagtgtg caactgccgg cccggccggc gtctctctag gtgtctcaca
    16141 agtcctgttc gttggctgcg ccaccctggc ccctgagcgt cgatgccgga cgatctcgcg
    16201 gacgtttatc gcaccaccta ccccgccctg gtgcgcttcc tgtaccgcaa ggtatgggac
    16261 gccgagcggg cggaagacct cgcgcaggag gccttctccc gcgcgttggc gcaccgtccc
    16321 gagaacccgc ggggctggct ttttgtcgtg gccgcaaaca tggcgcgtga cgaggcccgc
    16381 aaggcggcgc gcgagcgccg gcacctcacg ctcctcgccg cggagccgca ggaccatcga
    16441 ccgtcacctg aagagatcct cgatgacgag gcacgccggc gccgggtgcg tgaagccctg
    16501 gatcagctga ccccgcggga ccgcgaggtg ctgcttctct gggatgccgg actctcgtac
    16561 gacgagatcg c
//
LOCUS       DASUBP010000112         6370 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_207189, whole genome shotgun sequence.
ACCESSION   DASUBP010000112 DASUBP010000000
VERSION     DASUBP010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6370)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6370)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6370
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_207189"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1379)
                     /gene="lnt"
                     /locus_tag="VFU75_04100"
     CDS             complement(<1..1379)
                     /gene="lnt"
                     /locus_tag="VFU75_04100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HEU5466046.1"
                     /translation="MSRARVALLVGLGAVLLLFAYPPFHVPILSFVVLAPAVLLLREL
                     ESRGDTRLAFQWGWWYGFVSQGVVLYWLVVALWHFTPLSGLGYLVTIVMMGVFTGGVF
                     WLLTRLRMKAPMVPLWVTLPLVWTTLEWAVGHAGDVAFPWLGIGTSLTDAPVLVQWAD
                     LAGARGIGVWLMWCSVLLVEAGLSVRGHVRSAAWRGAAVMATVTASWAYGAHRMATLR
                     LRPAGVVGLVQPNIGFHEKWDPSEVQREVDLLMSLSRRVRAMSRPDVIAWPEAALPGF
                     LQQEPAWDSALSRFARESHTAMVIGGLYYTGVPSSDPGSYQMYNAAFYMDSTGHWGLY
                     PVYEKRYLVPVVERTPFVPLSWFKAIPGIANWSGRFHPGTKLPLYPTPVGKVGVIVCY
                     ESAFEDLTRTYRAEGADVVFNITNDAWYGETTAPYQHAAHLIMRAIETRMGIARAAND
                     GVSEFVDPLG"
     gene            complement(1376..2383)
                     /locus_tag="VFU75_04105"
     CDS             complement(1376..2383)
                     /locus_tag="VFU75_04105"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5466047.1"
                     /translation="MNDPIRVAIIGAGGIAQVAHLPVLRRLPGVEIAAICDNDLSKAQ
                     ALASRFEVKNTYDDIEEVLKYAEADAVVLCTPNHLHEIHAVAALSAGAHVLCERPLAL
                     KTEGVEVVLQAAERYGKRIMVGMNHRFRTDVQAVRNFVNGGELGTVDAIRGGWYTFQP
                     SRQQLGWRLHRPEAGGGAILDLGMPLIDLGLWLTGWPLPKRVSANVLGRSAGGVEDLG
                     SALIVCENGTAISVDVSWRHMGAGERFWFDVVGKKGAASIQPLRVFKDVHGTPTDVTP
                     TGASSRETVFQQSYRAEWTYFLAVLRGDVNAPPPKDQLALQRVMDALYRSAEEGRDVL
                     L"
     gene            complement(2380..2901)
                     /locus_tag="VFU75_04110"
     CDS             complement(2380..2901)
                     /locus_tag="VFU75_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466048.1"
                     /translation="MGASRLRGAMVGALRFDAGNIAPRVRVLLGLSYFRSNFNQSSLN
                     DFANKIRALVNDPDSNFTVNVGQIRWSDLAGDLDLQYAFPQSQTLTTFLGAGVGVHLR
                     HGSGSAINGTFVQDALDGLFPAANVTVGVEARLTSQVALSAEGRGVVSQGLSTASLSV
                     GLMYRFHGAGGPR"
     gene            complement(3062..4285)
                     /locus_tag="VFU75_04115"
     CDS             complement(3062..4285)
                     /locus_tag="VFU75_04115"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HEU5466049.1"
                     /translation="MRAARARRVLMAVALLGAAACNPHRPPPLPGAEPELRIGIADGL
                     QRITLGGDAQVLVTDDANGSAVAGIPAGSVWSIVPDSGALVLIAPDGARTGHHQGISA
                     VSVTNNHFVAADGKRYRGRVNVVRTGSGLQLINRIGLEGYVAAVIGPEMGPRHQDELQ
                     ALDAQAIVSRTFALKNRGRWEAQGFDLTDDTRDQVYGGVAAETPQAWAAVRRTAGQVV
                     MYHGDLIDAFFHSTCGFRTADATEAFRNGVARPYLRPVSDASGGGHYYCDISPRFRWR
                     EEWDGATLRTILARSIPDLQRIVNIEVSKTSPSGRVAELRIVFPHGDARIPEPDVRSV
                     LRSASGSLLQSAAFQVFVTTDSGQVTSLVAAGAGSGHGVGFCQWGAIGRARAGQGYHD
                     IITTYFPGTDIEKLY"
     gene            complement(4282..5598)
                     /gene="rimO"
                     /locus_tag="VFU75_04120"
     CDS             complement(4282..5598)
                     /gene="rimO"
                     /locus_tag="VFU75_04120"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HEU5466050.1"
                     /translation="MKLGVISLGCDKATVDSERLVGDLVGHGAVVTPDLPHSDVILVN
                     TCGFIDAAKQESINAILGAARLKQDGDVKAVVAVGCLVQRYKAELAAEMPEVDLFLGF
                     SDLHHLVPELAKRGLIADPVTGHPGVRQFLGDQPHVRYLKISEGCDHTCAFCAIPLMR
                     GKHRSEPVERLVREAQALEAQGAVEINLVAQDLGHFGRDLGLGGPKLPDLLEALLAQT
                     SVPWYRLLYVYSAGLSERLVRVIADNPRIVPYIDMPIQHANDQMLEKMRRPERKRTIV
                     EKLAWLRAAVPDLAVRTTCLVGFPGETDEQFRELVEFIEEAQFDRMGAFTYSPQEGTR
                     AMQYPDDVPEEVKRERLEELMEVQRAVSGDRLARFVGREVDVLIDRVADPLDGGATHV
                     GRVPWQADDVDGVTYVGAGGWARPGRMIRARVTESEDYDFRAVALA"
     gene            complement(5595..6098)
                     /locus_tag="VFU75_04125"
     CDS             complement(5595..6098)
                     /locus_tag="VFU75_04125"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEU5466051.1"
                     /translation="MASQSSFDISTGADLQEVDNAVNQATKEVMQRYDFKGSKASITF
                     DRTKGLLTLVADDDFKMKALFDILQGKMIKRGVPVKNLQLGEIVPATGDTVRREITLT
                     QGIPQDKAKAIIKAIKDKKFKKAQGSIQGEEIRVQSPSKDELQEVMAFLRTQDFGIEL
                     KYGNYRG"
     gene            complement(6223..>6370)
                     /locus_tag="VFU75_04130"
     CDS             complement(6223..>6370)
                     /locus_tag="VFU75_04130"
                     /inference="COORDINATES: protein motif:HMM:NF016432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HEU5466052.1"
                     /translation="LETPEPLTLEEIGARLGITRERVRQIKEKALARLRHVSRARALD
                     SFLF"
BASE COUNT         1091 a         2186 c         2083 g         1010 t
ORIGIN      
        1 cccaggggat ccacgaactc cgacaccccg tcgttggccg cccgtgcgat gcccatccgc
       61 gtttcgatgg cgcgcatgat caagtgcgcg gcgtgctggt agggtgcggt ggtttctccg
      121 taccaggcgt cgttcgtgat gttgaagacc acgtccgcac cctcggcgcg gtacgtcctc
      181 gtcagatcct cgaacgcgga ttcgtagcag acgatgaccc cgaccttccc gacgggcgtg
      241 ggatacaggg ggagcttggt cccagggtga aagcggccgg accagttggc gattccggga
      301 atggccttga accagctcag cggcacgaag ggcgtccgtt ccaccacggg gaccaggtac
      361 cgtttttcgt agaccggata caggccccaa tgccccgtcg agtccatata gaacgcggcg
      421 ttgtacatct ggtaggagcc ggggtcggag ctggggacac ccgtgtagta caaccccccg
      481 atcaccatcg cggtgtggct ctcgcgcgcg aagcgcgaca gcgcggagtc ccacgccggt
      541 tcctgctgga gaaatccggg tagggccgcc tccggccacg cgatgacgtc cggacgggac
      601 atcgcccgca cccggcgcga gagactcatc aggaggtcga cttcccgctg gacctcggat
      661 gggtcccatt tctcgtggaa accgatgttg ggctgaacca gtcccaccac gccggccggc
      721 cgcaggcgca acgtggccat ccggtgcgct ccgtacgccc aggacgcggt gacggtcgcc
      781 atcacggcgg caccccgcca cgccgcggac cggacgtggc cgcgaaccga aagcccggcc
      841 tcgaccagca ggaccgagca ccacatcagc cagacgccga tcccgcgcgc acccgccagg
      901 tcggcccact gcacgagcac cggcgcatcg gtgagagacg tgccgatccc cagccaggga
      961 aacgccacgt cgccggcgtg accaaccgcc cattccagcg tggtccagac gagcggcagc
     1021 gtcacccaga gcggaaccat cggcgccttc atccggagcc tggtgagcag ccagaatacg
     1081 ccccccgtga acacgcccat catcacgatg gtcacgaggt acccgagtcc ggacaggggg
     1141 gtgaagtgcc agagcgccac caccagccag tagaggacca ctccctgact gacgaacccg
     1201 taccaccagc cccactggaa ggcgaggcgg gtatcgccgc gcgactcgag ctcccggagg
     1261 agcaagaccg ccggggcgag gacgacgaac gagaggatcg ggacgtggaa cggaggatac
     1321 gcgaacagca ggaggacggc gcccaggccc accagaaggg ccacgcgcgc tctgctcaca
     1381 ggagcacgtc gcgtccctct tcggcggacc ggtagagggc atccatgacg cgctgcagcg
     1441 ccaactggtc cttgggagga ggtgcgttca cgtcaccgcg cagcaccgcc aggaagtagg
     1501 tccactccgc gcgatacgac tgctggaaga ccgtctcccg cgagctcgca ccggtgggcg
     1561 tcacatcggt tggcgtcccg tgcacgtcct tgaacacgcg gagcggctgg atcgaagcgg
     1621 cacccttctt ccccaccacg tcgaaccaga aacgctctcc cgcccccatg tggcgccagc
     1681 tgacgtccac cgaaatcgcg gtgccgttct cgcacacgat cagcgccgag cccaggtcct
     1741 ccacgcctcc cgcggaacgc cccaggacgt tcgccgacac gcgcttgggc agcggccacc
     1801 ccgtgagcca gagtcccagg tcgatcaacg gcatgccgag gtcgaggatc gcgcccccgc
     1861 ccgcttccgg gcgatggagt cgccatccca gctgctggcg ggagggctgg aaggtgtacc
     1921 agccgccgcg gatggcgtcg acggtcccca gctcgccgcc gttcacgaag ttgcgcacgg
     1981 cctgcacgtc ggtgcggaac cggtgattca tccccaccat gatccgcttg ccataccgct
     2041 ccgccgcctg gaggacgacc tccacccctt cggtcttgag cgccaggggc cgttcacaca
     2101 ggacgtgcgc gcccgccgac agggcggcga cggcgtggat ctcgtgcaga tgattcggcg
     2161 tgcagaggac caccgcatcc gcctcggcgt atttcaggac ttcttcgatg tcgtcgtagg
     2221 tgttcttgac ctcgaaccgg gacgcgagcg cctgcgcttt ggaaagatcg ttgtcacaaa
     2281 tcgcggcaat ctccacgccg ggcagcctgc ggagcacggg caggtgggct acctgcgcga
     2341 tcccgcctgc accgatgatg gcaacccgga tcggatcgtt caacgcgggc ctcccgctcc
     2401 gtgaaatcgg tacatgagtc cgactgacag tgacgcggtg gacaacccct gggacacgac
     2461 gccgcggccc tcggccgaca aggccacttg cgaggtgagc cgggcttcga cgccgaccgt
     2521 cacgttggct gctggaaaga ggccatcgag tgcgtcctgc acgaacgtgc cgttgatggc
     2581 cgagccggag ccgtggcgca gatgcacgcc gacgccggcc cctagaaacg tggtgagggt
     2641 ctggctctgg gggaaggcgt actgcaggtc caggtctccc gcgaggtccg accaccggat
     2701 ctgtcccaca ttcacggtaa agttcgagtc cgggtcattg acgagcgccc gaatcttgtt
     2761 cgcgaagtcg ttgagcgaac tctggttgaa gttcgagcgg aaataagaga gtcccaggag
     2821 cacccgcacc cggggggcga tgttgccggc gtcgaaccgc agagccccca ccatggcgcc
     2881 ccggagccgg ctggcgccca aggcaccgaa ctcgaaccgg agcccggtgg gttggaggtt
     2941 gtcgtaggag aactgctcca gaaaagactg ggatgataga cggctagacg ggtagacggg
     3001 taggagggcg aaagccgcga ggagacttct agccgtccaa ccgtctagcc gtctggtgtg
     3061 tttagtacag tttttcaatg tcggtccccg gaaagtacgt ggtaatgatg tcgtgatacc
     3121 cctgccccgc gcgggcgcga ccgatggcgc cccactggca gaaccccacg ccgtgcccgg
     3181 atcccgcgcc cgccgcgacg aggctcgtga cctgccccga gtcggtggtg acgaacacct
     3241 ggaacgcagc gctctgtaac agcgatcccg aggcggagcg gagcacgctg cggacgtccg
     3301 gctcgggaat ccgcgcatcc ccgtggggga acacgatccg cagctccgcc acgcgccccg
     3361 acggcgacgt ctttgacacc tcgatgttca cgatccgctg cagatcgggg atgcttcggg
     3421 ccaatatagt gcggagcgtg gccccatccc attcttcgcg ccaccggaac cgcggggaaa
     3481 tatcgcagta gtagtgcccg ccccccgacg cgtccgacac gggacgcagg tagggacggg
     3541 cgacgccgtt ccgaaacgcc tcggtcgcgt ccgcggtccg gaacccgcag gtcgagtgga
     3601 aaaacgcgtc gatcaggtca ccgtgataca tcaccacctg ccccgcggtc cgccgtacgg
     3661 ccgcccaggc ctgtggcgtc tcagccgcca cgcccccgta gacctggtcc cgcgtgtcgt
     3721 ccgtcaggtc gaacccctgg gcctcccagc gaccccggtt cttcagggcg aaggtgcggg
     3781 acacgatggc ttgggcgtcg agggcctgaa gctcgtcctg atgccggggc cccatttccg
     3841 ggccaatgac ggctgccacg tacccttcca gcccgatccg gttgatcagc tgcagtccgc
     3901 tgcccgtccg gaccacgttg accctgcccc ggtagcgctt cccgtcggcc gcgacgaaat
     3961 gattgttggt gacactcacc gcggagatgc cctgatgatg gccggtgcgc gcgccatcgg
     4021 gcgcgatcag caccagggcg cccgagtcgg gcacgatgga ccagacggac ccggcgggga
     4081 tgccggccac ggcggacccg tttgcgtcgt cggtgacgag gacctgagcg tcgcccccca
     4141 gggtgatccg ctgcaggccg tcggcgatgc cgatgcggag ctcgggttcg gccccgggga
     4201 gtgggggcgg acggtgggga ttgcaggcgg cggcgccgag aagcgccacg gccatgagga
     4261 cgcgtcgggc gcgggctgcc ctcacgccag cgcgaccgcc cggaaatcgt agtcttcgct
     4321 ctccgtgacc cgggcccgga tcatcctgcc ggggcgagcc cagccgcctg ccccgacgta
     4381 ggtgacgcca tccacatcgt ccgcctgcca gggcacccgt cccacgtgcg tcgcgccccc
     4441 gtccagcgga tcggccaccc ggtcgatgag cacgtccacc tcgcgcccga cgaaccgcgc
     4501 gaggcggtcc cccgacacgg cgcgctggac ttccatgagc tcctccaggc gctcgcgttt
     4561 cacttcctcg ggcacgtcgt ccgggtactg catcgcccgc gtgccctcct gcggggaata
     4621 cgtgaacgcg cccatgcggt cgaactgcgc ctcttcgatg aattcgacga gctcacggaa
     4681 ctgctcgtcg gtctcacccg ggaatcccac gaggcacgtc gtccgcacgg cgaggtcggg
     4741 caccgcggcg cgcagccagg ccagcttctc cacgatggtc cgcttccgct ccgggcggcg
     4801 cattttctcc agcatctggt cgttggcgtg ctggatgggc atgtcgatat agggcacgat
     4861 gcgcgggttg tcggcgatca cgcgcaccag ccgttccgac aaaccggccg aatagacgta
     4921 caggagccgg taccacggca ccgacgtttg cgcgaggagt gcctccagaa gatccgggag
     4981 cttgggacca ccgagcccga gatcgcgccc gaagtgtccc aggtcctgcg cgacgagatt
     5041 gatctccacg gcgccctgcg cttcgagggc ctgcgcctcg cgtaccaggc gctccaccgg
     5101 ctcggagcga tgcttcccgc gcatgagcgg gatggcgcag aaggcgcagg tgtgatcgca
     5161 cccctccgag atcttgagat agcggacgtg cggctggtcg ccgaggaact gccgcacacc
     5221 gggatggccg gtcacggggt cggcgatcag tccccgcttg gcgagctcgg ggaccagatg
     5281 atgaaggtcg gaaaatccga ggaagagatc cacttccggc atctcagcgg cgagctcagc
     5341 cttgtagcgc tggacgaggc acccgacggc gaccaccgcc ttcacatccc cgtcctgctt
     5401 caaccgggcg gcaccgagga tggcgttgat ggactcctgc ttggcggcgt cgatgaaccc
     5461 gcaggtgttg accaggatga cgtccgaatg cggcaggtcg ggcgtcacga cggccccatg
     5521 tcctaccagg tcccccacga ggcgctcgga gtccaccgtc gccttgtcgc acccgaggga
     5581 gatgacgccc agcttcatcc gcggtagttc ccgtacttca gctcgatgcc gaagtcctgc
     5641 gtgcgcagga aggccatgac ctcctggagc tcgtcctttg agggagactg cacccggatt
     5701 tcctctccct gaatggatcc ttgggccttc ttgaacttct tgtccttgat ggccttgatg
     5761 atggccttcg ccttgtcctg cggaatgccc tgcgtgagag tgatctcgcg gcggacggtg
     5821 tcccccgtcg cgggcacgat ctcccccagc tgcaggtttt tcaccgggac cccgcgcttg
     5881 atcatcttcc cctgcaggat gtcgaagagc gctttcatct tgaagtcgtc gtccgcgacc
     5941 agggtcagga gccccttggt ccggtcgaag gtgatcgacg ccttggaccc cttgaagtca
     6001 tagcgctgca tgacctcttt ggtcgcctga ttcacggcgt tgtcgacctc ctggaggtcg
     6061 gcgcccgtgg agatgtcgaa tgatgattgg gaggccattg gcggggaata taacgggagc
     6121 cgggagtcgg gagtcgtctg cctccatgaa acaggaagcc ccgcctgcct aagcggggct
     6181 tccacttttc ggactccgac gactcccggc tcccgactcc cgctagaaca ggaacgagtc
     6241 gagcgccctg gcccgcgaga catgccggag cctcgccagc gccttctcct tgatctgcct
     6301 cacccgctcc cgcgtaatgc ccagtcgcgc gccgatctct tcgagcgtga ggggttcagg
     6361 ggtttcgagg
//
LOCUS       DASUBP010000113         5238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_411590, whole genome shotgun sequence.
ACCESSION   DASUBP010000113 DASUBP010000000
VERSION     DASUBP010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5238
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_411590"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..253)
                     /locus_tag="VFU75_04135"
     tRNA            complement(181..253)
                     /locus_tag="VFU75_04135"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(218..220),aa:Trp,seq:cca)
     gene            complement(252..1331)
                     /locus_tag="VFU75_04140"
     CDS             complement(252..1331)
                     /locus_tag="VFU75_04140"
                     /inference="COORDINATES: protein motif:HMM:NF013166.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase domain-containing protein"
                     /protein_id="HEU5466053.1"
                     /translation="MTVSSFLQELRRDDIRVWTEGDTLRCTAPAGVLTPALRDRLLQE
                     KDAIVQFLRAADALARQERAIVPMQPEGTRTPVFAVPGHNGNVFSFRSLVRELGEDQP
                     FFGLQPPGVDGECKPLTSVEELAVYFATRIVAFRPDGPYIIVGHCAGGGIALELAHQL
                     LDRGADVPVLALFGSPYPTWFRFPPQLLEYLAPHVTWVSQHLRTLASLSNRERRLYVA
                     AKIAERRHQRRVAREARDDAMADPVLVQRSRIEAATRKALRRFRPRRYPGRVAVFLPN
                     REWRHRGHSMRWREVAGDCEMYSGPDGCEGDVMLHEPHVRLIAEQFRDCARRAEEAAG
                     PGRRLGDGEGVARGVDSVISSGINS"
     gene            complement(1328..>5238)
                     /locus_tag="VFU75_04145"
     CDS             complement(1328..>5238)
                     /locus_tag="VFU75_04145"
                     /inference="COORDINATES: protein motif:HMM:NF012873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HEU5466054.1"
                     /translation="LKINVEKSELDLLRGLSSTDWPKVRQLVIEVDQQEDLEPITTLL
                     EGQGFETLVEQDPLLRNTDLCYVYAIRPSADGAARLIRGQPQARVELPRDGEVLAPAT
                     LRAYLKARVPQYMIPSAFVLMERFPLNANGKIDRQALPAVSAETLPPARDVDRPLTET
                     ERALAGIWTDLLKVEGVGVDDDFFDLGGDSLLAIRAVSRIRDMFRVDLQTGALFENST
                     LGALAKLVADAGGATDDGPHLERRPRQGPCALSFAQEQLWFLSQLDPGSPAYNIVDVI
                     RLASYDATALHRALAEVVRRHEALRTIVCERDGHPVQVVLPEIDLAVPEVDLTALPAP
                     EREREWTRVVREEGRKPFDLSRAPLFRATVVHVSPREHRLLLTIHHIVADEWSMELIQ
                     QEVNQFYEAFTHGRPAPLPELPIQYADFASWQREQFKGPELHEELSFWKAQLTGAPTV
                     LDLVTDKPRPAVQSFRGGTEAFEIPRELMERVKALGREEQATLFMVLEASFAALLHRY
                     TGQDDLLVGTPISQRTHSQTERLVGCFLNTVVLRSTFTEELTFRSLVRQTRDRARGAY
                     AHAHVPFNAVVADLAPERGGSHSPLFQVMFVLHDAAGMSQVSKVSGNRQLETGTSKFD
                     ITMLVSETEQGVDGMIEYSTDLFDRQTIQRLCRHYTTLLEALARTPDESIAHASLLTA
                     AEHRQLLVGWNDTTTEFPDGHRYLHRLIEAQASRTPDRVAVVFENRQLTYGDLNHRAD
                     RLALELKGLGVGPDVRVGLLVERSPEMVVALVGILKAGGAYVPLDPAFPPDRLAYMVE
                     DSGMGMLVTHRDLDAALSVRPNVIVRLDSPADEMARPRGDRAVGSESSPEQLAYVLYT
                     SGSTGKPKGVEIPHAAIVNFLLSMQREPGFTANDALLAVTTLSFDIAGLELYLPLITG
                     GRVVIAGRDEVVDPLRLMELMRDSGCTVMQATPATWGALVSAGWTGSPGLKALCGGEA
                     LSRDLAEALLTRCGELWNLYGPTETTVWSTVERITSADVPVSIGRPIANTQVLVLDSH
                     RELVPAGLVGELYIGGAGVARGYLGRDELTRERFIPSPFSSGARLYRTGDLGRWLPDG
                     RLECLGRNDQQVKIRGFRIEPGEIESAIARHPTVREGAVVAREDGTGEKRLVAYLVAG
                     DAPADLIDQVRALIRAVLPEYMVPSEFVILGALPRTANGKLDRKALPAPAPGAVPAAG
                     AVAPRTPTEELVMAAFSDVVGRADFGVCDNFFDWGGNSLRAARLVSRLRAKAGVELLL
                     RHLFERPTVAGLAEVIDGLSWVGRAGAGAAEAGEREEIAL"
BASE COUNT          920 a         1848 c         1650 g          820 t
ORIGIN      
        1 attcccggcg cgccggcgac tcccggctat tggtatcgct gcaattgaag ttcggaattg
       61 acctgaaacg aagcgcggga gccaaggccc ccgcgctcac tgcttacagg cccggcagga
      121 atcgatgcgt agcacaaggc actggcgggc ctgcgaacac gagcgcacgc gagtgtgaga
      181 caggcccggc aggacttgaa cctgcaaccc ccggttttgg agaccggtgc tctaccaatt
      241 gagctacgga cctaggaatt gatgccgctg gaaattaccg aatcgacgcc ccgcgcaaca
      301 ccctctccgt ccccaagtcg ccgccccggc cccgcagcct cctcagcgcg gcgcgcacaa
      361 tcccggaact gctcggcaat cagccgcacg tgcggctcgt gaagcatcac gtccccctcg
      421 cacccgtccg gcccggaata catctcgcag tcgcccgcga cctcccgcca gcgcatcgag
      481 tggccccggt gccgccactc ccggttcggg agaaatacgg cgacccggcc ggggtaccgg
      541 cgagggcgaa atcgccgcag cgccttgcgg gtcgcggcct cgatcctgga ccgctgaacc
      601 agcaccgggt ccgccatggc gtcgtctcga gcctcccgcg ccacccggcg ctggtgccgg
      661 cgttcggcga tcttggcggc gacatagagc cgacgctccc ggttggacag cgatgccagc
      721 gtacggagat gctggctcac ccatgtcaca tgcggggcca gatactccaa gagctgcggc
      781 gggaatcgga accaggtcgg atacgggctg ccgaacagcg ccagaaccgg gacgtccgca
      841 ccgcgatcca gcagctggtg cgccagctcg agcgcgatgc ccccaccggc gcagtggccg
      901 acgatgatgt agggaccgtc cggacggaat gccacgatcc gcgtagcgaa gtagacagcc
      961 agttcttcca cggaggtgag gggcttgcac tcgccgtcca cccccggagg ctgcagccca
     1021 aagaacggct gatcctcgcc gagctcccgc accagggacc ggaacgagaa cacgttgcca
     1081 ttgtggccgg gcacggcaaa caccggtgtc cgtgtgccct cgggctgcat cggcacaatc
     1141 gcccgctcct gccgagccag cgcgtccgcc gcacgaagga attgaacgat ggcgtccttt
     1201 tcctgtagca gccggtcccg aagggccggc gtcaacaccc cggcaggcgc agtacatcga
     1261 agggtgtccc cctccgtcca cacgcggatg tcgtcgcggc gcagctcctg aaggaaagac
     1321 gatacggtca cagggcgatc tcttcgcgct caccagcttc ggcggccccg gcccccgccc
     1381 tccccaccca tgacaaccca tcgatgacct cggccagccc cgcgaccgtc ggccgctcga
     1441 acagatggcg gagcaagagc tcgacccccg ccttggcacg aagccgggaa accaggcgag
     1501 ccgcccggag cgaattgccg ccccagtcga aaaagttgtc gcatactccg aaatccgcgc
     1561 ggcccaccac gtccgagaac gccgccatca ccagctcttc agtgggcgta cgcggcgcta
     1621 cggcgccggc agccggcacc gcaccgggcg ccggcgccgg aagtgccttc cggtccagct
     1681 tcccgttggc ggtgcgcggc aacgctccga ggatgacgaa ctccgagggc accatgtatt
     1741 ccgggagcac cgcacgaatg agcgcgcgca cctggtcgat caggtcggcc ggcgcgtccc
     1801 ccgccacgag ataggccacc aaccgcttct cgccggttcc gtcctcgcgc gcgacgaccg
     1861 caccctcgcg caccgtgggg tggcgagcga tggccgattc gatttctccc ggttcgatcc
     1921 gaaagccgcg gatcttcacc tgctggtcgt tccgccccag gcactcgagg cggccatccg
     1981 gcagccaccg tcccagatcc ccggtgcgat acaggcgcgc cccggacgag aaggggctcg
     2041 ggatgaagcg ctcgcgagtc agctcgtcgc gcccgaggta gccgcgcgcg acgcccgcgc
     2101 caccgatgta cagttcgccc accaggcccg cgggaacgag ctcgcgatgg gaatcgagca
     2161 ccagcacctg cgtattcgcg atgggccgac cgatcgacac cggcacatcg gcggacgtga
     2221 ttctttcgac agtcgaccag acggtggtct cagtcgggcc atagaggttc cacagctcgc
     2281 cgcaccgggt cagcaaggct tccgccaggt cccgggacag cgcttcgccg ccgcacagcg
     2341 ccttcagacc aggcgacccc gtccacccgg cgctgaccag cgccccccac gtggcgggcg
     2401 tggcctgcat taccgtgcat ccggaatccc gcatcaactc catcaggcgc aggggatcga
     2461 cgacctcatc ccgtccggca atgaccaccc gcccgcctgt gatcaggggc agatacagct
     2521 cgagtcctgc gatgtcgaat gagagggtcg tcacggcgag cagtgcgtca ttcgccgtga
     2581 aaccaggctc tcgctgcatg gacaagagga agttcacgat cgcggcatgc gggatctcca
     2641 ctcccttggg cttcccggtc gagccggatg tgtacagcac gtacgccagc tgttccgggc
     2701 tcgactccga ccccacggct cgatcgccgc ggggccgcgc catctcgtcc gcaggcgaat
     2761 ccagacgcac gatcacgttc ggccgcacgc tcagcgcagc gtccaggtcg cggtgcgtca
     2821 ccagcatgcc catcccactg tcttccacca tgtaggcgag gcggtccggt ggaaaagccg
     2881 gatcgagcgg cacgtatgcc ccgcccgcct tgagaatccc caccagcgcc acgaccatct
     2941 ccggggagcg ctcgaccagg agacccaccc gcacgtccgg accgaccccg agccccttca
     3001 gctcgagcgc caaccgatcg gcgcgatggt tcaggtcgcc gtatgtcagc tgccgattct
     3061 cgaacaccac cgctacccga tcgggcgtgc gactcgcctg ggcttcgatc agccggtgca
     3121 ggtaccgatg cccatccggg aattccgtcg tcgtatcgtt ccagcccacc agcagttgcc
     3181 ggtgctcagc ggcggtgagc agggacgcgt gcgcaatgct ttcgtccgga gtccgggcga
     3241 gagcttcgag caaggtcgta tagtgccggc acaggcgctg gatggtctgc ctgtcgaaca
     3301 agtcggtgct gtactcgatc atcccgtcga caccctgctc cgtctccgaa accagcatcg
     3361 tgatgtcgaa cttcgacgtg ccggtttcga gctgccggtt gcccgacact ttcgatacct
     3421 gcgacatgcc cgcggcgtcg tggagcacga acatgacctg aaacagaggc gaatggctcc
     3481 caccccgctc gggcgcgaga tcagctacga cagcattgaa gggaacgtgc gcatgtgcat
     3541 atgctccccg agcccggtcg cgcgtctgcc gtaccaggct ccggaacgtc agctcttcgg
     3601 tgaacgtgga tcgcagcact accgtgttca ggaagcagcc gaccagacgc tcggtttggc
     3661 tgtgggtccg ctgggaaatc ggggttccaa cgagcaggtc gtcctggccg gtatagcggt
     3721 gcagcagcgc cgcaaagctt gcctcgagca ccatgaacag cgtggcctgc tcttctcgcc
     3781 ccagcgcctt cacccgctcc atgagctcac gaggtatttc gaacgcctca gtcccacctc
     3841 ggaagctttg gactgcggga cgaggcttgt ccgtgacgag gtccaagacg gtcggtgccc
     3901 ccgtcagctg ggccttccaa aacgaaagct cctcgtgcaa ctcaggcccc ttgaattgct
     3961 cgcgctgcca ggacgcaaag tccgcgtact gaatcggcaa ctcgggcaag ggcgccggcc
     4021 ggccgtgtgt gaacgcctcg tagaattgat tgacctcctg ctggatcaac tccatcgacc
     4081 attcgtcggc gacgatgtga tggatggtga ggagcaggcg atgttcgcgg ggggacacgt
     4141 gtaccaccgt tgcccggaac agtggcgcgc gagacaggtc gaatggcttg cgcccctcct
     4201 cacgtaccac gcgggtccat tcgcgctccc gctccggcgc cggcaatgcg gtgagatcga
     4261 cctctggtac ggccaggtcg atctccggca gcaccacctg gacggggtgg ccgtcgcgtt
     4321 cacagacgat ggtgcgcaac gcctcgtggc ggcggaccac ctccgcgagc gcgcgatgca
     4381 acgcggtggc gtcgtatgaa gccagcctga tgacgtccac gatgttgtac gccgggctgc
     4441 cggggtcgag ctgactcagg aaccagagct gttcttgcgc gaacgagagc gcgcagggcc
     4501 cctgccgggg gcgccgctcc aggtgcggcc catcatccgt cgcgcccccg gcgtcggcca
     4561 cgagcttcgc cagcgcgcct agggtggagt tctcgaacag cgcgcccgtt tgcagatcca
     4621 cccggaacat gtcccggatg cgggataccg ccctgatcgc cagcagcgaa tcgccgccca
     4681 ggtcgaagaa gtcgtcatcg accccgacac cttccacctt gaggaggtcg gtccaaatgc
     4741 ccgcgagggc ccgctcggtc tccgtgagcg ggcggtccac atcacgcgcc ggtggcaagg
     4801 tctcggctga gaccgccggg agcgcctggc gatcgatttt cccgttggcg ttgaggggaa
     4861 accgttccat caggacgaac gccgacggga tcatgtactg ggggacccgg gccttgaggt
     4921 aggcgcgcag cgtcgcgggt gccagcacct ccccgtccct gggaagctcc acccgggcct
     4981 gcggttgccc ccggatgagg cgcgctgccc cgtccgctga cggcctgatc gcgtacacgt
     5041 agcacaggtc ggtattccgc agcagcgggt cctgctcgac cagggtctcg aatccctgcc
     5101 cctcgagcaa ggtcgtgatc ggctcgagat cctcctgttg atcaacttcg atcaccagct
     5161 ggcgcacttt gggccagtcc gtggaggaca ggcctcggag gaggtcgagc tcgctctttt
     5221 cgacgttgat cttgagca
//
LOCUS       DASUBP010000114         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_43910, whole genome shotgun sequence.
ACCESSION   DASUBP010000114 DASUBP010000000
VERSION     DASUBP010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43910"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VFU75_04150"
     CDS             <1..699
                     /locus_tag="VFU75_04150"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5466055.1"
                     /translation="LLHGYANSLEGWSGLGDSLARDHEVIALDLRGHGQSTKFTRQSD
                     YGRYIADDVVHLLDHLHIRRAHLVGHSMGALVAADIVERYPNRVASATLLAGPFWPDS
                     AAAAHAVERWDVDLDAGRGLTALMLWLVPGMDTATAQGINHDLMAANDPRALRAVLPT
                     LGALTVGLSHAPAVPVLIAAGGVDPLREYSHALASHWPRARMLDLPTANHVTILSRPQ
                     VLAGIRELISARRD"
     gene            complement(702..>2466)
                     /locus_tag="VFU75_04155"
     CDS             complement(702..>2466)
                     /locus_tag="VFU75_04155"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEU5466056.1"
                     /translation="PLTRHLRSVQQAPRALSRVVAALGSDEQTQLLTLAVIIGAAAGG
                     SVVVFYKTIDEIQSLVLRGALGVPIPAVVLIPAFVALGIGACRLLMRWGAKDSPGENI
                     PDVMYRLAVKGGVIRFAPVLAKTLGAALVIGTGGSVGAEGPVVVLGAATGSRIGRWLK
                     ASPNRLRTLVGCGAAAGISAAFNAPIAGVIFGIEKILGAAGGMALGPFVVASILAATV
                     GRLAFGNHPVLALPAAFAFGSPWELFLYVGLGIVCGVVSVLYSRGVWRTQDWSDRLRS
                     PWVRIALGTLVVGGLDLAFRADLWGHGHESIDLGVLMGRGALFLLALAGAKLVATAAT
                     FGAGGTGGVFTPALYIGATLGAAYGVAVHGVLPMSAEGPGALALVGMAGLVAGATHAP
                     LTAIMMVFEMTGDYGLILPLMLTSVISYGIARRLHPESIYTEWLVRRGVVLSNGADAA
                     VLTRISVHECLNPNPTVIRNDAPLEEIRRIIQENRGTDFPVLDGNGALVGMLSAVGLR
                     DALADGSPPGLVLAADLTIPETDPITGDDTLLTTLRRFGKRDADVLPVVDPARRDRLV
                     GVVSRQDLLGAYERALTAEGH"
BASE COUNT          432 a          833 c          834 g          367 t
ORIGIN      
        1 ctgcttcacg gctacgcgaa cagcctcgag ggttggtcag gtctcggtga ttcgctggcc
       61 cgcgatcatg aggtgatcgc gctggatctg cgtggccacg gtcaaagcac caagttcacg
      121 cgacagagcg attacggacg atacatcgca gatgacgtgg tgcacctgct cgatcacctc
      181 catatccgcc gggcgcatct ggtgggccac tcgatgggag cgctcgtcgc cgccgacatc
      241 gtagagcggt acccgaaccg cgtcgcgagt gccacgctcc tggcggggcc cttctggccg
      301 gattcggctg cggcggcaca cgcggtggag cgatgggacg tcgacctcga cgccgggcgg
      361 ggcctcacgg cgctgatgct ctggctggtt cccggcatgg acaccgcaac ggcgcaaggg
      421 atcaatcatg atctgatggc agcgaacgac ccccgcgccc tgcgtgcggt gttgcctaca
      481 ctcggtgcgc tgacggtcgg tctaagccac gcgcccgccg tgccggtcct gatagctgcg
      541 ggtggagtgg accccttgag ggagtattcg catgccctgg cgagtcactg gccccgggcg
      601 cgcatgctcg acctcccgac ggccaatcac gtgaccattt tgagccggcc tcaggtgctt
      661 gccggtatcc gcgaactgat cagcgcgcgg cgtgactaga gtcaatgccc ctcggccgtc
      721 aacgctcgct cgtaggctcc cagcaggtct tgtcggctga cgacgccgac cagtcgatcc
      781 cgtcgagccg ggtcaacaac tggtagtaca tcggcgtcgc gcttgccgaa ccggcgcagc
      841 gtggtcagca gcgtgtcgtc tcccgtgatg ggatccgtct cggggatcgt caagtcggcc
      901 gcgaggacaa gccctggcgg agagccgtcc gcaagggcat cgcggagacc gaccgcggac
      961 agcatgccga ccagggctcc attcccgtcg agcaccggga aatccgttcc ccgattctcc
     1021 tgaatgattc gcctgatctc ttccagggga gcgtcgttcc ggatgacggt gggatttggg
     1081 ttcaggcatt cgtgcaccga gatccgcgtg aggacggcag cgtccgcacc attgctcagg
     1141 accaccccac gacggaccag ccactccgtg tagatcgact ccgggtgcaa tcgccgcgcg
     1201 atgccgtagg agatgacgct cgtcagcatc aggggaagga tcagaccgta atctccggtc
     1261 atctcgaaga ccatcatgat cgcggtgagc ggcgcgtgag tcgcaccggc cacgagtccc
     1321 gccatgccca ccagcgccaa cgccccgggc ccctcggcgc tcatggggag cactccgtgg
     1381 accgccacgc cgtacgccgc gcccagggtg gcgccgatgt acagggcggg ggtgaacacg
     1441 ccgcccgttc ctccggcccc aaacgtcgcc gcggtcgcga ccagctttgc ccccgcgagc
     1501 gcgaggagaa agagtgctcc ccgtcccatc agcacgccca ggtcgatgct ctcgtgccca
     1561 tggccccaga gatcggcgcg gaaggccagg tccaggcccc cgaccacgag cgttccaagg
     1621 gcgatccgga cccatggtga ccgcaggcgg tcagaccagt cctgcgttct ccagacgccg
     1681 cgggaataca ggaccgacac cacgccgcat acgatgccga ggccgacgta caggaacagc
     1741 tcccagggag acccgaacgc gaaggccgcc gggagcgcca gtaccgggtg attcccgaag
     1801 gcgagccgac caactgtcgc cgccaggatg ctggcgacca cgaagggccc gagcgccatg
     1861 cctccagccg cgccgaggat cttctcgatc ccgaagatca cgcccgcgat gggcgcgttg
     1921 aaggcggccg aaattccggc ggcggctccg cagccgacca gggtgcggag ccggttgggg
     1981 gacgctttga gccagcgtcc gattcgggaa cccgtggccg caccaaggac gacgaccggt
     2041 ccttccgcgc cgaccgagcc gccggttccg atgacgagcg cggcaccgag cgtcttcgcc
     2101 agcacggggg cgaaccggat cacgccgcct ttgaccgcga gccggtacat gacgtccggg
     2161 atgttctcgc ccggagagtc cttcgcgccc cagcgcatga ggaggcggca cgccccgatg
     2221 ccgagggcga cgaacgcggg aatgaggacg acagcgggga tggggacccc gagggcgccg
     2281 cgcagcacca atgattggat ctcgtcgatg gtcttgtaga agacgaccac cgaaccgccc
     2341 gcggcggcac cgatgatgac ggcgagagtc aggagttggg tctgctcatc gcttcccaag
     2401 gcggcgacga ctcgagagag cgcgcggggc gcctgctgta cgcttcggag atggcgggtg
     2461 agaggg
//
LOCUS       DASUBP010000115        15644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_698029, whole genome shotgun sequence.
ACCESSION   DASUBP010000115 DASUBP010000000
VERSION     DASUBP010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 15644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15644
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_698029"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="VFU75_04160"
     CDS             complement(<1..793)
                     /locus_tag="VFU75_04160"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HEU5466057.1"
                     /translation="MFTALSEQLSGVLKRLTGRGVLSEADVTEALREIRRHLLEADVS
                     FEVTRDFVDRVKERAVGALKIDTVRPGQQVVKLVHDEIAGMLGGARRDLSFASVGPTV
                     ILLVGLQGSGKTTTAAKLARRLKLEQKAPGLVAADVYRPAAAEQLEQLGKQVGVPVFG
                     GPKGLPDGRREPGAVASLVRDALRQAEHARCRTVIVDTAGRLQIDAELMDELKALKDT
                     VKPKDVLLVADGMTGQDAVKIAKGFHESVGLTGAILTKMDGDSRGG"
     gene            853..1716
                     /locus_tag="VFU75_04165"
     CDS             853..1716
                     /locus_tag="VFU75_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466058.1"
                     /translation="MPEPLWWQGLTMIGVGTLAGGITSSVNVWLLFHPKEPRRLGPLT
                     LQGTIPKYHARIAESVDRWVSEGLASAALEAGARHFITAQRDALLHDERPLLERLPRG
                     LLAAVEQGITDYLPLAVEQFGATLEDPEARTRITEALKGVLDHAVANLKAHERLVAKL
                     VVTPERVDRMVAELGLVFESPEFKARLSKTVSDAVMRFLRTPIAERLWTLGPDRLDGL
                     ESAASDYVVAVLKAPETADWAAARAREAADALRDYPIGLPRSLKRSVALGFALGALIG
                     ALGWGLTLLLS"
     gene            complement(1702..2907)
                     /locus_tag="VFU75_04170"
     CDS             complement(1702..2907)
                     /locus_tag="VFU75_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466059.1"
                     /translation="MTRVGGLVAALVTVAACRQRAEAVQTQTAVRDLVRQTMPAVEAA
                     VGLKFKREPGVEIRTRDQIRSYVLHKLDEDLPPAEFASYEAAYKHFGLIPDSMDLHKL
                     LVNVLTEQVAGYYDPDSGALFIPTDADTATLRTTVISHELVHALQDQYSPIDSVIHQR
                     HQNDRRSAAQAIFEGQATLGQIRVLMPEQKIENLPSLWDMGGAVQMQQEQFPNFSNAP
                     LWVKQTLIFPYLAGGDFVKWFIAEHPGEQPYGKLMPVSTSQILHPDRFAAHELPVRIA
                     FDPGADRPDLTDDLGEFETRLVLQQALGSADQATSLANGWAGDRYAVFGRHAEALVWY
                     VVWRNQDAANEFAAWLAKGWARRVEPGRTWRVDKLSLDGRPATVVVDAPVGWTGFKHL
                     PAVHISAQQ"
     gene            complement(2904..4040)
                     /locus_tag="VFU75_04175"
     CDS             complement(2904..4040)
                     /locus_tag="VFU75_04175"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5466060.1"
                     /translation="MSSPFNLFGEGDVMARVALDLTRQARRGELEPVRCRDAEIARVV
                     DILLRQGKNNPALVGRAGVGKTAIVEGLAQRIAAGDVPPALKDAQILALDHVALLADT
                     SFRGQYEGRLRRVVESLAANPDSILFVDELHNLVGQGTAVGSAMDAGNMLKPALVRGD
                     IRIIGATTGAEYDRWIKSDPALERRFHPVAIEELNAAQTREVLLARRPRLERHHAVAI
                     SDDALQAAIVLTDQFVLDRARPDKAIDVLDEACAHAQATAKVSPDLERLIRERRQVEK
                     RIRQGSAEDPAGEPGADLAAEIVPMLERIGAELDRMFSGERGAPTPSPASRTAQTPQT
                     LGDRRAELDRLLRAELESQGLVVTGREIARVVGASVGKDIEWAG"
     gene            complement(4045..4830)
                     /locus_tag="VFU75_04180"
     CDS             complement(4045..4830)
                     /locus_tag="VFU75_04180"
                     /inference="COORDINATES: protein motif:HMM:NF013632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEU5466061.1"
                     /translation="MGVEAFFLIVATLFGLVFGSFLNVCIIRLPNEDPKARSLIRPPS
                     SCPKCHKLIAWYDNIPIVSWLLLRGKCRWCGNPISPQYLIIEGAVGLLWLICALWYGP
                     TPRMIDGCVLGTILLGIAIIDARHYIIPDELSYGGLVVGLALALFGGATGLVDALIGA
                     AAGWALLWVVRTVGGYVLKQEAMGWGDVKMLAMIGAFVGWRGVLLTVFLGALAGSVIF
                     LPLQLMGKKRLVPFGVFLAVGGAVTFLFRDSMLSWYMQFLHGA"
     gene            complement(4820..5509)
                     /locus_tag="VFU75_04185"
     CDS             complement(4820..5509)
                     /locus_tag="VFU75_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEU5466062.1"
                     /translation="MRLLEEALAAGVPIHGAVIAADLERTSRGASLREDLEKHAVPIE
                     AVSARVFAPLADTDAPQGIVAVIEPRRWTVDDLKLDAGGVVQVIDGVQDPGNVGTLLR
                     TAHGLGAQGTIALRGTADILGPKTLRAAMGANFRHPVVSLDDAAFITWARVAGVTLWA
                     TASDGVPIGRVKRGTGPIGVIVGNEGAGIRPALNAVAAQRVAIPMAGGAESLNVGVAA
                     GILLYEVLRGR"
     gene            5660..6475
                     /gene="thiD"
                     /locus_tag="VFU75_04190"
     CDS             5660..6475
                     /gene="thiD"
                     /locus_tag="VFU75_04190"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008902 - hydroxymethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0008972 - phosphomethylpyrimidine kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEU5466063.1"
                     /translation="MRIALTVAGSDSGGGAGIQADLKTFHQFGVFGTSVIAAVTAQNT
                     KGVAAWEPVSDALIARQLDALAQDLPPDGIKTGMLGSRAAIETVAAALQRMPTKPYVL
                     DPVLVATSGHALADETAAAAMVEHLVPLATLVTPNLDEAARLTGLEVTTAEQMERAGR
                     ALLKRGTAAALIKGGHLEGDAVIDVLVRPEGVRRFLHPRIATSSTHGTGCTLSAAITA
                     GLALGRNLERSVEDGIDFVQRAIAAAPGLGAGKGPVNHFVPVPRGAPNDLLGG"
     gene            complement(6551..7348)
                     /locus_tag="VFU75_04195"
     CDS             complement(6551..7348)
                     /locus_tag="VFU75_04195"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEU5466064.1"
                     /translation="MAADAVARRIAPRKPKVAIVLGSGLGFLADRVEGAVRVPYADIP
                     GFPEPGVQGHKGELVAGNLGGVPVLVQAGRFHLYEGHDAVTAGLPVRVFARLGIKTLV
                     VTNASGGIRPGFGAGTLMLLSDHLNLMFRNPLIGPLVDGEERFPDMTEAYDVGLRKLA
                     ISTAEQLKVPLEEGVYAALLGPSYETPAEVRMLGRMGADAVGMSTVPEVIVARARGMR
                     CLGFSIVTNPAAGLSGAALSHQEVLEVGANAGARLGDLITAVVRKLD"
     gene            complement(7402..8814)
                     /locus_tag="VFU75_04200"
     CDS             complement(7402..8814)
                     /locus_tag="VFU75_04200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005337 - nucleoside transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:1901642 - nucleoside transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEU5466065.1"
                     /translation="MTWRRVVMAFGVTFLCSLLVFMLPRAVWNTLHAQTPAAAQATAA
                     PAPMPQAPADTAARVAAPRSIPQRLTGLIGMFVILGIGVALSRNRRAISWRVVAWGLG
                     LQFVFAVFVLRVPWTRDAFSKLGYVVTKLLGFSYAGSEFVFGELGKQHSSIGIVFAFQ
                     VLPTIIFVSALFAIMYYLGIMQVVVKAFAIVMNKVLGASGAESLNVAASIFMGQTEAP
                     LTIRPFLPKMTQSELMTVMTSGMAHVSGSIMAAYIAFGISAEHLLTAVIMTAPGTIMM
                     AKIFEPETQVPETQGSVRLDVPRTDVNVVDAASRGTVEGLHLMLNVIAMLISFIALIA
                     LTNGILGVVHGWLPWFPADLQTILAWIFRPIAWVMGVPWRDSGTIGGLLGTRMVLNEF
                     IAYSQLGPLKGALDPVSFTIATFALCGFANVSSVGIQIGGIGALAPDRKHDLAKLGFR
                     AMLAGTLANFLSATLAGMLL"
     gene            complement(8811..10061)
                     /gene="add"
                     /locus_tag="VFU75_04205"
     CDS             complement(8811..10061)
                     /gene="add"
                     /locus_tag="VFU75_04205"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEU5466066.1"
                     /translation="MAEPSLRAFVNERPVEVKRGARVRDAIAALDLRLADQVAAGEAY
                     VTDARGIQVAGDDPVGEAGSVYRVVVSARPGGAALSKEMLRRWPKVELHVHLDGCLRP
                     ETMIELARMQGVKLPADTPDALAAKLSVKGAKNLEEYLLSYGTTLSVMQTPEALERIA
                     YEFVQDVAAENVRYVEVRYSPLLHRPAMTLTEAIEAPLRGFERGMAETGTKVGVIVCG
                     IRTRSPAESLELAKAAAGYRSAGVVAYDLAGAERGHPAGEHRAAFEYAAGHGMFCTCH
                     AGEGAGADSVSEAIHVCGATRIGHGTRVGEDSGLLAEVVEKQIALEMCLTSNLHTHTV
                     ETLATHPARKYLAQGARVTLNTDGRLMDGITLTDEYHLAHTALGFTKAELARVVLNGC
                     ESAFLPEYERVALVARIQSDLEAL"
     gene            complement(10061..13198)
                     /locus_tag="VFU75_04210"
     CDS             complement(10061..13198)
                     /locus_tag="VFU75_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5466067.1"
                     /translation="MTTIAALLLLLQTTTAPKQSPPAATTPDTAHLVIVATTDLHGRL
                     LGWDDVRDAEAPGGLSRAATILSTLRSQYPGQVVLVDAGDLIEGNLYATYYGRVAPAR
                     PHPMIDALNALDYDAAIPGNHEFDFGVDVLRNAVADATFRYLSANIMVPATQAERADA
                     PLFSTTAVIVRNGIRIGITGLTTPGVMVWDRQQLGGKIKVLPIAQAAPAALAGLDRQG
                     VDFKLVLVHAGLNEASSYDTTGVGPENNAAALAGMPSKPDLVVFGHTHREVKDSVING
                     VHFVQPGFWGRSLSVVHVSLKRTNGKWAVDQMRADLIPLATVAELPSFVRREGPSHER
                     VRIWADQTLGNAAAGFEGRYSRAEDTPLIDFVNEVMRRHAGADLSATPSFDPGASFPG
                     EIHLRDLAGVYPFDNTLMAVRITGRQLKEFLEQSAKYFRTYVPGQAVINPAVPGYNYD
                     IVAGVDYQIDLSQPEGQRIRGLLYKGRPVTGVDTFTIALSNYRAAGGGGYDMLKGAKV
                     VYDRGEDIRDLLAAEIKKDVNVTAAAYYHRNWSILTPAYAAVRQTFGPAAPPVSAADS
                     TMLRVVAITDLHGQLAARTWDWSQGRPVGGAVALKVWIDSLSKDCGCTAVRLDGGDEM
                     QGTPVSNFAFGRPVIRAMNDFEIDAAALGNHEFDWGIDTLRARMGEATYPFLSANITD
                     ASGARPSWIEPWTIISRGGLKIVVMGLTTTTTPTSTDPRNVAGLTFGDLATAVKRELP
                     AARKAGNFVIVLAHEGAICDSGACHGDIIDLARQLDSGSVDLIVAGHTHRRVDTRVNG
                     IPIVEAASSARDVAVADFIRSGGRCCTVRTKVIDAYADQVGNDTAVASLVSHAQARVD
                     SLTRRPVASLRFALVRKGDEYPLGHLIADAQLNVGRGDVSIMNNGGIRADLPAGTVTY
                     GDLFQVQPFGNKLVKLTISGAVLLSALDWAVAGGQERAQLGGVEVWYDSRKEPGKRIT
                     KTRLTNGRKIEAKQTYTLIVSDFMAAGGSGFAMLKGVPAVAVGITDLDALISYLRVLP
                     QPVDAPAEARFHLEH"
     gene            13325..14050
                     /locus_tag="VFU75_04215"
     CDS             13325..14050
                     /locus_tag="VFU75_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5466068.1"
                     /translation="MNLSARVLVVDDEADITALVAYHLAKAGYRVSTAASGVEALNAV
                     REERPDLVVLDLMLPGRSGFDVLSELRGRPETKDVGVVLLTAKKDEPDRIKGLSLGAD
                     DYLAKPFSPQELVLRVGAVLRRLTAPAVSAGGVLAAGPVRLDVAAHKAQVDGDAVDLT
                     ATEFRLLRLLIEREGRVQDRTQLLETVWRAQPDIQTRTVDMHVQRLRTKLGRAGSCIE
                     TVRGVGYRFRTPNPPPAARTKKR"
     gene            14050..>15644
                     /locus_tag="VFU75_04220"
     CDS             14050..>15644
                     /locus_tag="VFU75_04220"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEU5466069.1"
                     /translation="MRLATRFFLATSALVTLAVATLTISADHTLRRELVTRLEGALEN
                     DARLIAAQVPADSNTWSEVAHRLGTVIGRRVTLIDPTGRVRGDADFPPEALPHLQNHL
                     MRPEVQQALRQGVGTYQRLSASTNERQLYVAVREGPPGIAIVRVSATLASVDTAVWET
                     ERTIIGVGIIAVLGAALVAWIMGLSLSRPLVQLAGSARAIAAEGEPVFPDSRIREVAD
                     HVRALRAMHEELQNRFSDLRSEREESRTLVESMTDGVIAADSAGRIGTCNAAARRMLD
                     RKSDEPIPELALLFLEKGSRDFVRDVVAGKEVDERELIVGGRVLLGTGRMLPDGGTLF
                     VLRDITALRHLETVRRDFVANVSHELKTPLTSIAGYAETIVSEAEAESPARHFAGTIL
                     ANAKRMQRLVEDLLDLSRIESGGWHPVPRVVDLSASARDAWTAFAETAQDRGVTFDQK
                     VPAALSIAVDPDALRQILTNLYDNSLRHTPRGGTITVWAAPAGEGVVLEVRDTGSGIG
                     AEHIPRIFERFYRADPARSRDQGG"
BASE COUNT         2599 a         5474 c         5095 g         2476 t
ORIGIN      
        1 cgccgccgcg ggagtcgccg tccatcttgg tgagaatcgc gccggtcagg cccacgctct
       61 cgtggaatcc cttggcaatc ttcaccgcgt cctggccggt catgccgtcg gccaccagca
      121 ggacgtcctt cggcttcact gtgtccttca gcgccttgag ctcatccatc agctcggcgt
      181 cgatctgcaa ccggcccgcc gtgtcgacga tgaccgtccg gcaccgcgcg tgctctgcct
      241 gccggagcgc atcgcgtacc aaggaggcga cggctcccgg ctcccggcga ccgtcaggga
      301 gtcctttagg gcccccgaaa actgggaccc caacctgctt cccgagctgc tcgagctgtt
      361 ccgccgccgc tgggcgatac acatccgccg ccaccagacc cggtgccttc tgctccagct
      421 tcaaccggcg ggccagcttc gccgccgtcg tcgtctttcc cgaaccttgc aacccgacga
      481 gcagaatcac cgtcgggcct acggaggcga aggagaggtc ccgacgagcc ccgccgagca
      541 tgccggcgat ttcgtcgtgg accagcttca ccacctgctg gcccggccgg acggtgtcga
      601 tcttgagcgc cccaaccgcg cgctccttca cccgatcaac gaagtcccgg gtcacctcga
      661 agctcacgtc cgcttcgagc aggtggcgcc ggatctcgcg cagcgcctcc gtgacgtccg
      721 cttcggacag caccccccgg ccagtcaacc gcttcagcac gccggagagc tgttccgaaa
      781 gggccgtaaa cataagcctt acaaattact tagcgagttg ctcgaaacca agtggggggt
      841 taagaatcga ccatgccgga acccctgtgg tggcaagggc tgaccatgat cggcgtcggc
      901 acgctggcag gtgggatcac ctcgagcgtg aacgtctggc tcctctttca cccgaaggag
      961 ccccgccggc tgggcccgct cacgcttcag ggaaccatcc ccaagtacca cgcccgcatc
     1021 gccgagtccg tcgatcgctg ggtgtccgag ggcttggcct cggcggccct cgaggcgggg
     1081 gcccggcatt tcatcaccgc tcaacgggac gccctcctcc acgatgaacg ccccctcctc
     1141 gaacgcctgc cccgcggcct cctggcggcc gtcgagcagg gaatcaccga ctacctgccg
     1201 ctggccgtcg agcaattcgg cgccacactc gaagatccag aggcgcgcac gcgcatcacg
     1261 gaggcgctga agggtgtcct cgaccacgcg gtggcgaatc tcaaggccca cgaacgtctc
     1321 gtggcgaagc tcgtcgtcac gccggagcgc gtggaccgga tggtcgccga gctgggactg
     1381 gtgttcgagt cacccgagtt caaggcccgc ctctcgaaaa cggtgagcga cgccgtgatg
     1441 cgcttcctcc ggacgcccat cgccgagcgc ctgtggaccc tgggacccga ccgtctggac
     1501 ggattggaga gcgccgcctc ggactacgtc gtcgcggtgc tcaaagcccc ggaaacagcc
     1561 gattgggctg cggcacgcgc ccgtgaggcg gccgatgcgc tgcgcgacta cccgatcggg
     1621 ttgccgcggt cgttgaagcg gagtgtggcg ttgggattcg cgctgggagc gctcatcggc
     1681 gcccttgggt ggggactgac cttactgttg agctgagata tggaccgcgg gaaggtgttt
     1741 gaatccggtc catcccaccg gcgcatcgac cacgaccgtc gcgggtctgc cgtcgagtga
     1801 cagcttgtcc acccgccagg tccgacccgg ctcgacccgc cgcgcccatc ctttggcgag
     1861 ccatgccgcg aactcgttcg ccgcgtcctg attccgccag accacgtacc agaccagcgc
     1921 ctcggcgtgt cggccgaaca ccgcatagcg gtcccccgcc cacccgttcg ccagactggt
     1981 tgcctgatcc gcggagccca aggcctgctg gagcaccaag cgggtttcga actcccccag
     2041 atcatctgtc aggtcgggtc gatccgcccc cggatcaaac gcgattcgga cggggagctc
     2101 gtgcgccgcg aagcggtcgg gatggagaat ttgcgaggtc gacaccggca tcagctttcc
     2161 gtacggctgc tcccccgggt gctccgcgat gaaccacttc acgaagtccc cgccggcaag
     2221 gtacgggaaa atcagcgtct gcttcaccca cagcggtgcg ttcgagaagt tcggaaactg
     2281 ctcctgctgc atctggaccg ctccgcccat atcccacaga gacggcaggt tctcgatctt
     2341 ctgttcgggc atcagcacgc ggatctgacc gagggtcgct tgcccttcga atatcgcctg
     2401 cgcggcactg cggcggtcgt tctgatgacg ctggtggatg accgagtcga tgggggagta
     2461 ctgatcttgt aaggcgtgca cgagctcgtg ggaaatcacg gtggtccgca acgtggccgt
     2521 atcggcatcg gtgggaatga acagcgcgcc cgagtccggg tcgtagtagc ccgccacctg
     2581 ctcggtcagg acgttcacga gcagcttgtg caagtccatg ctgtcaggga tcaggccgaa
     2641 gtgcttgtag gccgcctcgt agctggcgaa ttcagcaggc gggagatcct cgtccagctt
     2701 gtggagcaca tagctccgga tctgatcccg ggtgcggatc tccactcccg gctcccgctt
     2761 gaacttgagt ccgacggccg cttccacggc gggcatcgtc tggcggacga gatcacgcac
     2821 ggccgtttgg gtctgcaccg cttcggcacg ctggcggcag gccgccaccg tgaccagcgc
     2881 cgccacgagc cccccgaccc gcgtcatccc gcccactcga tgtccttgcc gaccgatgct
     2941 ccgacgaccc gcgcgatctc acgccccgtc acgacgagtc cttgcgactc gagctccgcc
     3001 cgcaacagcc gatccagctc ggcgcggcgg tcgcccagcg tctggggagt ctgggcagtc
     3061 cgggacgccg gggatggcgt tggggccccg cgctcccccg aaaacatccg gtccaattcg
     3121 gccccgatcc gctcgagcat ggggacgatc tccgccgcca ggtcggcgcc gggctccccc
     3181 gcggggtcct ccgcgctccc ctgccggatt cttttctcca cctgccggcg ctcacgaatc
     3241 agacgctcca ggtcgggcga caccttggcc gtcgcctgcg catgggcgca cgcttcatcc
     3301 aggacgtcga tcgccttgtc ggggcgggcg cggtcgagca cgaactggtc ggtcagcacg
     3361 atggcggcct gcagggcgtc gtcggagatg gccacggcgt ggtgccgctc gagccggggg
     3421 cgccgcgcca gcagtacctc tctcgtctgc gccgcattca gctcttcgat cgccaccgga
     3481 tgaaagcgcc gctcgagcgc gggatcgctc ttgatccagc gatcgtattc ggcgcccgtg
     3541 gtcgccccga tgatccggat gtcgccgcgc accagggccg gcttgagcat gttccccgcg
     3601 tccatcgccg accccacggc ggtgccctgt cccacgaggt tgtggagctc gtccacgaac
     3661 aggatcgaat caggattggc ggcgagcgac tcgaccaccc ggcgcaggcg gccttcgtat
     3721 tggccgcgga acgaggtgtc ggccaggagc gcgacgtgat cgagcgcgag aatctgcgcg
     3781 tctttcaggg ccggcggcac gtctcccgcg gcgatacgct gggcgaggcc ttcgacgatg
     3841 gccgtcttgc cgacccccgc ccggcccacg agcgcagggt tgttcttccc ctgccggagg
     3901 aggatgtcga cgactcgcgc gatttcggcg tcccggcagc gcacgggctc gagctcgccg
     3961 cggcgggctt gccgcgtgag gtcgagcgcg acgcgcgcca tcacatcgcc ttcgccgaac
     4021 agattgaagg ggctgctcac cggactacgc cccgtgcagg aactgcatgt accaggacaa
     4081 cattgaatcc ctgaaaagga aggtcacggc tccacccacg gcgaggaata ccccgaaggg
     4141 caccagtcgc ttcttgccca tcagctgcag ggggagaaag atgaccgacc cggccagcgc
     4201 gcccaggaac acggtgagca acacgccgcg ccatccgacg aacgcgccga tcatcgccag
     4261 catcttcacg tcgccccacc ccatcgcctc ctgtttcagg acgtagccgc ccaccgtccg
     4321 taccacccag agtagcgccc accctgcggc ggcaccgatc agggcgtcga ccagaccggt
     4381 cgcaccgccg aacagcgcga gggccagccc caccaccaat cctccatacg agagttcgtc
     4441 gggaatgatg tagtggcggg cgtcgatgat ggcgatgccg aggaggatgg ttcccagcac
     4501 acagccgtcg atcatccggg gcgtggggcc gtaccacagg gcgcagatga gccagaggag
     4561 acccaccgcg ccctcgatga tgaggtactg gggagagatg gggttaccgc accaccggca
     4621 tttgccccga agcagcagcc aggagacgat cgggatgttg tcgtaccagg cgatgagctt
     4681 gtggcacttc gggcacgatg acggcggccg gatcagggag cgggccttcg gatcctcatt
     4741 cgggagccgg atgatgcaga cgttcaggaa gctcccgaac accagtccga acagcgtcgc
     4801 gacgatcaga aaaaatgctt caacgcccac gcaatacctc gtagaggaga atgcccgccg
     4861 ccacaccgac gttgagcgac tccgcgcccc ccgccatcgg gatggcgacg cgctgggccg
     4921 ccaccgcgtt caacgccggc ctgatccccg cgccctcatt gcccacgatg acgcctatgg
     4981 ggcccgtgcc gcgcttgacc ctcccgatgg gcacgccgtc cgacgcggtg gcccacaagg
     5041 tcacgccggc gaccctggcc caggtgatga acgccgcatc atcgagggac accacggggt
     5101 gccggaagtt cgcccccatc gccgcgcgaa gcgtcttggg ccccaggatg tcagccgttc
     5161 cccgaagcgc gatggtgccc tgggcaccga ggccgtgggc ggtacgcaag agggtgccga
     5221 cgttgccggg atcctggacg ccatcgatca cctgcactac accgccggcg tcgagcttca
     5281 ggtcgtcgac ggtccagcgg cggggttcga tcacggcgac gatcccctgc ggggcgtcgg
     5341 tgtcggcgag gggcgcgaac acccgtgccg agacggcttc aatggggacg gcgtgcttct
     5401 cgagatcctc gcgcagcgat gcgccgcgcg acgtgcgttc gaggtcggcg gcgatcaccg
     5461 ccccgtggat ggggaccccg gccgcgagcg cctcctccag caggcgcacg ccctccacca
     5521 gtgcaagtcc gcggcgcccc cgcgccttgc gccgttgtag atcacggatc agagactgca
     5581 gattagtacc tatgggcctc cccgtgcctc ataacatcgc cgcaagactc cctttcgcca
     5641 agcacgaaga ggccaaaccg tgagaatcgc cctgaccgtc gcgggctcgg attcgggagg
     5701 gggggccgga attcaggcgg acctcaagac cttccatcag ttcggcgtgt tcgggacgag
     5761 cgtcatcgcc gccgtcacgg cccagaatac gaagggcgtc gccgcctggg aacccgtgag
     5821 cgacgcgctg atcgcccggc agctcgacgc gctcgcccag gaccttccac ccgacggcat
     5881 caagaccggc atgctcggat cgcgcgccgc catcgagacg gtcgcggcgg cgctccaacg
     5941 catgccgacc aaaccctacg tcctggatcc ggtgctggtc gcgacgtcgg gacacgcact
     6001 cgcggacgag acggcggcgg cggccatggt ggagcatctg gtcccgctcg ccacgctggt
     6061 gaccccgaac ctcgatgagg cggcgcggct cacgggactg gaagtcacga cggccgagca
     6121 gatggagcgg gcggggcgcg ccctcttgaa gcgcggcacc gccgccgcgc tcatcaaggg
     6181 gggacacctc gaaggcgacg cggtgatcga cgtgctggtc cggccggagg gggttcggcg
     6241 cttcctgcac ccgcgcatcg ccaccagctc gacgcacggc accgggtgca ccttgtcggc
     6301 cgcgatcacc gcgggactcg ccctgggccg caacctcgaa cgctcggtgg aagatggaat
     6361 cgatttcgtg cagcgggcga ttgcggcggc accggggctg ggtgccggga aaggaccggt
     6421 gaaccatttt gttccggtgc cccggggagc tccgaacgac ctgctggggg gataagggga
     6481 acgcggaact gggaactcgg aaggcggaac ctccacccct gttccgcatt ccgacttccg
     6541 cattccgcgt tcagtccaac tttctaacca ccgcggtgat caagtcacca agcctggcac
     6601 ccgcgttcgc tccgacctcc agcacttcct ggtgagatag tgcggccccc gacaatcccg
     6661 ccgcgggatt cgtcacgatc gagaacccca ggcaccgcat cccccgcgcg cgtgcgacga
     6721 tcacttccgg caccgtggac atccccaccg cgtcggcccc catcctcccg agcatgcgca
     6781 cctcggcggg cgtctcgtac gagggaccca ggagcgccgc gtagaccccc tcctcgagcg
     6841 gcaccttgag ctgctccgcg gtggatatcg cgagcttcct caaacccacg tcatacgcct
     6901 cggtcatgtc gggaaaccgc tcttcaccgt cgaccagcgg tccgatcagg ggattccgga
     6961 acatcaggtt gaggtgatca gagaggagca tcagggtgcc ggctccgaag ccgggtctga
     7021 tgccaccgga ggcgttcgtc acgaccagcg tcttgatgcc cagccgggcg aagacgcgca
     7081 ccgggaggcc ggcggtgacg gcgtcgtggc cctcatagag atgaaagcgt cccgcctgca
     7141 cgaggacggg caccccgccc aggttgccgg cgaccagctc tcctttgtgg ccctggacgc
     7201 cgggctcagg gaatcccgga atgtccgcat acggcacccg taccgcccct tcgactcggt
     7261 cggccaggaa gcccagtccc gagcccagga cgatcgcaac cttgggcttg cggggtgcga
     7321 tgcggcgcgc gaccgcgtcg gcggccacct ggggcgatgc cccggacggc ccggacggcc
     7381 cagacggccc agacgccccg ctcaaagcag catgcccgcg agggtggctg acaggaagtt
     7441 ggcgagcgtc ccggcgagca tggcgcggaa gccgagcttc gccaggtcgt gcttccggtc
     7501 aggggccagc gcgccgatgc cgccgatctg aattcccacc gacgacacgt tggcgaatcc
     7561 gcagagggcg aacgtcgcga tggtgaagga cacggggtcg agagccccct tcagtggacc
     7621 gagctgggaa tacgcgatga actcgttcaa caccattcga gtgccaagga ggccaccaat
     7681 ggttccggaa tcgcgccacg gcacccccat gacccaggcg atgggccgga agatccaggc
     7741 gaggatggtc tgcagatcgg cggggaacca cgggagccat ccgtgcacaa cgccgaggat
     7801 gccattggtc aaggcgatca aagcgatgaa cgagatgagc atcgcgatca cgttcagcat
     7861 gagatgcagg ccttcgaccg tcccgcgtga cgccgcgtcc accacgttca cgtcggtcct
     7921 gggaacgtcg agcctcacgc ttccctgagt ctccggaacc tgcgtctcag gctcaaaaat
     7981 cttggccatc atgatggtac ccggagcggt catgatcacg gcggtcagca ggtgctcggc
     8041 gctgataccg aaggcgatgt acgccgccat gatggaaccc gagacgtggg ccatgccgga
     8101 agtcatcacg gtcatcagct ccgactgcgt catcttggga agaaagggcc ggatggtgag
     8161 tggggcctcg gtctggccca tgaagatcga cgccgcgacg ttcaggcttt ccgccccgct
     8221 cgcccccagg actttgttca tgacgatggc gaacgccttc accaccacct gcatgatgcc
     8281 caggtagtac atgatcgcga agagggccga cacgaagatg atggtgggaa ggacctgaaa
     8341 ggcgaacaca ataccgatgc tcgagtgctg cttgccaagc tccccgaaca cgaactccga
     8401 tcccgcgtag gagaagccca ggagcttggt caccacatag cccaacttgg agaaggcgtc
     8461 ccgggtccag ggcacccgaa gaacgaacac cgcgaagacg aactgcaacc ccagtcccca
     8521 agccacgacg cgccagctga tggcgcgacg gttccgggag agcgcgaccc cgattccgag
     8581 aatcacgaac atcccgatca ggcccgtcag ccgctgcggg atgctgcgcg gagccgcgac
     8641 gcgagcggcc gtgtccgcgg gcgcctgtgg cattggtgcc ggggcggcgg tggcctgcgc
     8701 cgccgccggc gtctgtgcgt gcagcgtgtt ccacaccgcc cgcgggagca tgaacaccag
     8761 aagcgaacac aggaaggtca cgccgaacgc catcaccacg cgtcgccagg tcaaagagcc
     8821 tccaggtcgc tttggatgcg cgccaccagg gccacgcgct cgtactcagg gagaaacgcg
     8881 ctctcacagc cgttcaggac cacccgcgcc agctcggcct tcgtgaaacc gagcgcggtg
     8941 tgggccagat gatactcgtc ggtgagcgtg atgccgtcca tcagccgccc atcggtattc
     9001 agcgtgaccc gggcaccctg agccaggtac ttccgggccg gatgggtcgc caacgtctcg
     9061 acggtatggg tatggagatt cgacgtcagg cacatttcga gcgcgatctg cttctcgacc
     9121 acttcggcga gcaggcctga gtcctctccg acgcgggtcc cgtgcccaat ccgcgtcgcc
     9181 ccgcacacgt ggatcgcttc gctgaccgag tcggcccccg ccccctcccc cgcatgacag
     9241 gtgcagaaca tcccgtgtcc cgccgcgtac tcgaacgccg cccggtgctc ccccgccgga
     9301 tggccccgct cggcgccggc gagatcgtac gccaccaccc ccgcactccg gtacccggcc
     9361 gctgctttag cgagctcgag cgattcggcg ggcgagcgcg tgcggatgcc gcagacgatg
     9421 acgccgacct tcgtaccggt ctccgccatg ccgcgctcga agcctctgag cggggcctcg
     9481 atcgcttcgg tcagcgtcat cgccggccgg tgcaggagcg gcgagtagcg cacctcgacg
     9541 taccgcacgt tttcggcggc gacatcctgg acgaattcgt acgcgatccg ctcgagggcc
     9601 tcgggcgtct gcatcacgga gagcgtcgtc ccgtaggaga gcaggtattc ctcgagattc
     9661 ttcgcgccct tcaccgagag cttggcggcc agggcgtcgg gagtgtcggc cggcagcttc
     9721 accccctgca tccgcgccag ctcgatcatc gtttcgggac gcaggcaccc gtcgaggtgg
     9781 acgtgaagct cgactttggg ccagcgacgc agcatttcct tcgacaacgc cgcgcccccg
     9841 ggacgggcgg acaccaccac gcgatagacc gaccccgcct caccaaccgg gtcatcgccg
     9901 gcgacctgga tgccgcgcgc gtcggtgacg tacgcctcgc cggcggccac ctggtcggcc
     9961 aggcgcagat ccagtgcggc gatcgcatcc cgcacccgtg caccccgctt cacctccacg
    10021 ggccgctcgt tcacgaaagc ccgcagggac ggctcagcca tcaatgctcc aggtggaatc
    10081 gggcctccgc gggggcatcc accggctggg gcaagactct gagataggag atgagggcgt
    10141 ccagatccgt gatcccgacc gccaccgcgg gcacgccctt cagcatggcg aatccggagc
    10201 cccccgccgc catgaagtca ctgacgatca gggtgtaggt ctgcttggcc tcgattttcc
    10261 tgccgttggt gagccgggtc ttggtgattc gcttcccggg ttccttccgc gagtcgtacc
    10321 agacctcgac gccgccgagc tgcgcccgtt cctggccccc cgccaccgcc cagtcgagtg
    10381 ccgagagcag gacggctccc gagatcgtca gcttgaccag cttgttgccg aagggttgga
    10441 cctggaacag gtccccgtac gtgaccgtcc ccgcgggcag gtcggcgcga atgccgccgt
    10501 tgttcatgat ggacacatcc ccacggccca cgttcagctg cgcgtccgcg atcaggtgac
    10561 cgagcgggta ttcgtcaccc ttccggacca gcgcgaaccg gaggcttgcg acggggcggc
    10621 gggtgaggga gtcgacccgg gcctgagcgt gcgagaccag gctggccacc gcggtgtcgt
    10681 tcccaacttg atccgcgtag gcgtcgatga ctttcgtgcg gacggtgcag caccgtcccc
    10741 cggaacgaat gaaatcggcc accgccacgt cgcgggcgct cgaggcggct tcgacgatcg
    10801 ggatgccgtt cacccgtgtg tcgacccggc ggtgggtgtg ccccgcgacg atcaggtcca
    10861 ccgaacccga gtcgagctgc cgcgccaggt cgatgatgtc gccatggcag gcgcccgaat
    10921 cacagatcgc accctcgtgc gccagtacga tcacgaagtt ccccgccttc cgcgccgcgg
    10981 ggagctcgcg cttcacggcc gtcgcgaggt cgccgaaggt caggcccgcg acgttccgcg
    11041 gatccgtgct cgtcggcgtc gtcgtcgtgg tgagacccat gaccacgatt ttcaggccgc
    11101 cacgggagat gatggtccac ggctcgatcc aggagggccg cgcacccgag gcgtcggtga
    11161 tgttcgcgga aaggaaggga taggtggcct cgcccatcct ggcgcgcagg gtatcgatgc
    11221 cccagtcgaa ctcgtggttg cccagcgccg cggcatcgat ctcgaaatcg ttcatcgccc
    11281 gaatcacggg ccggccgaac gcgaagttcg acaccggggt accctgcatc tcgtcgccgc
    11341 cgtcgagacg gaccgcggtg catccacaat ccttcgagag cgaatcgatc cacaccttca
    11401 gcgccaccgc tccgcccacc ggccttccct gagaccagtc ccaggtccgg gccgccagct
    11461 ggccgtggag gtccgtgatc gccacgaccc ggagcatcgt cgaatcggcg gcggagacgg
    11521 gcggcgccgc cggcccgaaa gtctgccgca ccgcggcgta cgcgggcgtc aggatggacc
    11581 agttccggtg gtagtaggcg gccgcggtga cgttgacgtc cttcttgatc tcggccgcga
    11641 gaagatcccg gatgtcctcg ccccggtcgt agacgacctt ggcgcccttc agcatgtcgt
    11701 agccgccacc cccggccgcg cgatagttgg acagcgcgat cgtgaacgtg tccacgcccg
    11761 tcaccggacg ccctttatag aggaggccgc ggatgcgctg cccctcgggc tggctcaagt
    11821 cgatctggta gtcgaccccc gccacgatgt cgtagttgta gccgggcacg gccgggttga
    11881 tcacggcctg ccccggaacg taggtccgga agtacttcgc gctctgctcg aggaactcct
    11941 tcagctgccg gccggtgatt cgcaccgcca tcagcgtatt gtcgaatgga tagacccccg
    12001 ccaggtcgcg gaggtgaatt tcgcccggga aggacgcgcc cggatcaaac gacggggtcg
    12061 cggaaaggtc ggccccggcg tggcgccgca tgacctcgtt cacgaagtcg atcagcggcg
    12121 tgtcctcggc ccgcgagtag cgtccctcga atccggctgc ggcgttcccc agtgtctggt
    12181 cggcccagat ccggacccgc tcgtgcgacg gcccctcccg gcgcacgaat gacggcagct
    12241 cggcgacggt cgcgagcgga atgaggtccg cccgcatctg gtccaccgcc cacttgccgt
    12301 tcgtccgctt gagcgagacg tggaccacgg agagcgagcg cccccagaat cccggctgaa
    12361 cgaagtgaac cccgttgatc acgctgtctt tgacttcccg atgcgtgtgg ccgaagacca
    12421 cgaggtcggg cttcgagggc atccccgcga gtgcggcggc gttgttctcc ggccccaccc
    12481 ccgtggtgtc gtaggacgac gcttcgttca ggcctgcatg cacgagcacc agcttgaagt
    12541 ccacgccctg ccggtccagg cccgcgagcg cggcgggtgc cgcctgcgcg atggggagaa
    12601 ccttgatctt ccccccgagc tgctggcgat cccaaaccat gaccccgggg gtggtgaggc
    12661 ccgtgatgcc gatccggatc ccgttccgga cgatcaccgc cgtcgtcgag aagagcggcg
    12721 catccgcccg ctccgcctgc gtcgccggaa ccatgatgtt ggcgctgaga tatcggaacg
    12781 tcgcgtcggc gaccgcgttg cggagtacgt ccacgccgaa gtcgaactcg tggttccccg
    12841 gaatcgccgc atcgtagtcc agggcgttca gcgcgtcgat catcggatgc ggacgagccg
    12901 gcgccacgcg cccgtagtag gtcgcgtaga ggttgccttc gatcagatcc cccgcatcga
    12961 ccagaacgac ctgtcccgga tactgcgagc ggagggtcga gaggatcgtc gcggcccggg
    13021 agagcccgcc cggtgcttcg gcgtcccgga cgtcgtccca gccgagcaag cgcccgtgga
    13081 gatcggtcgt cgcgacgatc accaggtggg ccgtgtcggg cgtggttgcg gcgggcggag
    13141 actgcttggg tgcggtcgtc gtctgcaaga gcagcagcag tgcggcaatt gtcgtcatag
    13201 tgcggtctaa attgccaagc cacttcgctt cgcgctacct tcgtcacagt cgtgtcacgg
    13261 gcatccggca gtcgtccccg gcaccgatat cttcatgcgc tatgccaaca ccctcgagta
    13321 accggtgaat ctgtcggcac gagtcctggt cgtcgatgac gaagcggaca tcaccgcctt
    13381 ggtggcgtat cacctggcca aggcgggcta ccgcgtcagc accgccgcct cgggtgtcga
    13441 ggccctcaac gccgtccgtg aggagcgccc cgatctcgtg gtgctcgatc tgatgctccc
    13501 cggccggtct ggcttcgatg tcctgtccga gctccgcggc cggccggaga cgaaggacgt
    13561 cggtgtcgtc ctgctcacgg ccaagaagga cgaacccgac cggatcaagg ggctctcgct
    13621 cggcgccgac gactatctcg ccaaaccatt ctcgccgcag gaattggtgc tgcgcgtggg
    13681 agccgtactt cggcggctca ccgcgcccgc cgtcagcgcc ggtggcgttc tggcggcagg
    13741 tcccgtccgt ctcgacgtag cggcccacaa agcccaggtg gatggcgatg ccgtggacct
    13801 gacggccacc gagttccggc tgctgcgcct gctcatcgag cgtgaaggcc gggtccagga
    13861 tcgcacgcag ctcctcgaga cggtctggcg ggctcagccc gacattcaaa cccggacggt
    13921 cgacatgcac gtgcaacgtc tgcggaccaa gctcgggcgc gccggctcct gcatcgagac
    13981 ggttcgcggc gtgggctacc ggttccggac gcccaacccg ccgcccgcgg cccgaaccaa
    14041 gaagcgctga tgcggctcgc cactcgcttc ttcctcgcca ccagcgcgct ggtgacgctc
    14101 gcggtggcga cgctcaccat ttccgccgac cacacactgc gccgggagct cgtgacccgt
    14161 ctggagggcg cgctcgagaa cgacgcgcgc ctgatcgccg ctcaggtgcc ggcggactcc
    14221 aacacctggt cggaggtcgc ccaccggctg ggtacggtga tcggccgccg cgtcaccctg
    14281 atcgacccga ccggccgggt ccgcggagac gccgacttcc cgccggaagc cctgccccac
    14341 ctccaaaacc acctgatgcg tcccgaggtc cagcaggctc tcaggcaggg cgtcggcacg
    14401 tatcagcggt tgtcggcctc gacgaatgaa cggcagctct acgtcgccgt ccgggagggc
    14461 cctcccggaa tcgcgatcgt acgcgtgtct gccaccctcg ccagcgtcga tacggcggtg
    14521 tgggagacgg aacgcacgat catcggcgta ggaatcatcg ccgtcctcgg cgccgcactg
    14581 gtcgcgtgga tcatggggct ctccttgtct cgtcccctgg tacagctcgc cgggagcgcc
    14641 cgggccatcg cagccgaagg ggagcccgtg tttcccgact cccggatccg tgaggtcgcc
    14701 gaccacgtgc gcgcgctgcg ggccatgcac gaggagctcc agaaccgctt ctcggacttg
    14761 cggagcgagc gggaggagtc gcggacgttg gtggagtcca tgaccgatgg cgtcatcgcg
    14821 gcggattcgg ccggccgcat cggaacctgc aacgccgccg cccgccggat gctcgaccgg
    14881 aagtcagacg agccgatccc cgagctcgct cttcttttcc tcgaaaaggg ctcgcgggac
    14941 ttcgtccgcg acgtcgttgc gggcaaggaa gtggacgagc gcgagttgat cgtgggcgga
    15001 cgcgtgctgc tcggcaccgg ccgcatgctg cccgacggtg gcacgttgtt cgtcctccgg
    15061 gacatcaccg ctctccgcca cctcgaaacc gtccgccgcg acttcgtcgc gaacgtgtcg
    15121 cacgaattga agacgccctt gacctccatc gccggatacg ccgaaaccat cgtctccgaa
    15181 gcggaagccg agtcacctgc gaggcatttc gccggcacca ttctcgccaa tgccaagcgc
    15241 atgcagcggc tggtggagga tctgctcgat ctttcaagaa tcgagtcggg tggctggcat
    15301 ccggtgccac gcgtcgtgga cttgagtgcg tcggctcggg atgcctggac ggccttcgcg
    15361 gaaaccgcgc aggatcgcgg ggtgaccttc gaccagaagg tgcctgccgc gctctcgatc
    15421 gcggtagatc ccgacgcgct ccggcagatc ctcacgaacc tgtacgacaa ctcgctgcgg
    15481 cacacgccgc gcggcggcac catcacggtg tgggcagcgc ccgccgggga gggtgtggtc
    15541 ctcgaagtcc gggacacggg gtccggtatc ggggccgaac acattccccg catcttcgag
    15601 cggttctatc gcgccgaccc cgcgcgctcc cgggaccagg gagg
//
LOCUS       DASUBP010000116        37714 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_589079, whole genome shotgun sequence.
ACCESSION   DASUBP010000116 DASUBP010000000
VERSION     DASUBP010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 37714)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37714)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37714
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_589079"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1750
                     /gene="uvrB"
                     /locus_tag="VFU75_04225"
     CDS             <1..1750
                     /gene="uvrB"
                     /locus_tag="VFU75_04225"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEU5466070.1"
                     /translation="YYQPEAYVPQTDTYIAKDSSINDDIDRLRLRATSSLIEREDVIV
                     VASVSAIYGLGDPELYKQLMVTVDVGAERGRDAILEDLVRIQYTRNDVNLERGVFRVR
                     GDTIEVFPAYEEQGVRIELWGDTVERVTKFDPVTGNAIVDLKRAAIYPATHFVTQRPS
                     VERAVASIRAELKDRLAELRVQNKLVEAQRLEARTTFDVDMLLEVGRCSGIENYSRHL
                     RGSAPGERPACLLDYFPSDFLCVIDESHQTLPQIGGMYEGDRSRKQTLVDYGFRLPSA
                     LDNRPLKYDEFFRLTPRMVFVSATPGEEELKLSGGVVVEQIIRPTGLIDPEVEIRPVK
                     GQVDDLLHEVRERAKVSERVLVTTLTKRMAEDLTDYLQQQAVRVRYLHSEIDAIERVE
                     IIRGLRLGEFDVLVGINLLREGLDLPEVSLVAVLDADQEGFLRSDRSLIQTVGRAARN
                     VHGRAILYADRMTGSMQRALDEMSRRRTIQQAYNVEHGVTPTSIVKSVLDVRFVTRVA
                     DARVGRPETGGAKKPADAREREALIALLESQMQAAAEQLDFEAAALLRDQILDLKAES
                     DAGRRAPHRGRAQPVR"
     gene            1713..2117
                     /gene="tsaE"
                     /locus_tag="VFU75_04230"
     CDS             1713..2117
                     /gene="tsaE"
                     /locus_tag="VFU75_04230"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEU5466071.1"
                     /translation="MRLTADELNRFGEELGRTLQAPAVLGFSGELGAGKTTLVQAICR
                     GLKAESPATSPTYALVHRYLAPGGPVYHVDGYRLKAPADARDLGFEEMEREGAIVLIE
                     WPERALGWAPALDRHFHLAHDADPDRRSLEEE"
     gene            2114..2851
                     /gene="tsaB"
                     /locus_tag="VFU75_04235"
     CDS             2114..2851
                     /gene="tsaB"
                     /locus_tag="VFU75_04235"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEU5466072.1"
                     /translation="MSALMLAVDTATDIASVAVGDGSGVNSGAHQQGSRRHAAEIVRL
                     IDFALSRAGAQIADLDGVVVGDGPGSFTGLRIGWAAAKGLVQESGADVIAVPSLMAAA
                     AGAAERVGSVPVAACFDALRGQVYGALYVVHADRVDTIVPPTLATIAELEGLAGARRP
                     ALAVGDGAVRFGAEVTKWTGVPPVPLDQLPPSAVVMLALAGRPGAGRRIEDFGTAEPE
                     YGRPAEAQVKWEARHGRQLPRSTDAPR"
     gene            2862..3278
                     /gene="rimI"
                     /locus_tag="VFU75_04240"
     CDS             2862..3278
                     /gene="rimI"
                     /locus_tag="VFU75_04240"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEU5466073.1"
                     /translation="MQGIEAVSFSDPWSARDFLETLATGVIFLAAVDDGGGVLGYLVA
                     RQMADEGEILNVGVDPQRRRIGVGRALVGAALARLRSLGARQVYLEVRESNLAARALY
                     RGFGFAEVSRRVKYYRRPVEDAILLRAAIPAGEGPR"
     gene            3381..3866
                     /locus_tag="VFU75_04245"
     CDS             3381..3866
                     /locus_tag="VFU75_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEU5466074.1"
                     /translation="MSRSLNKVILIGNLGADPEVRTTNNGSKVATVSLATGRQWTGQG
                     GDKQEKTEWHRVVMWNNKTGNGLADLAEKFMKKGDRIYVEGRIEYRTWEDKEKQTRYT
                     TEIIAREVMLLSPRGGGGEGGDFSRAPAKATAKGGAEGKPESLDSFPEALDAEDDDLP
                     F"
     gene            3895..5190
                     /locus_tag="VFU75_04250"
     CDS             3895..5190
                     /locus_tag="VFU75_04250"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU5466075.1"
                     /translation="MAVLLAVPLLGARLSAQDTTKAKPAPTDTTKAKAPADTASSLLQ
                     QAATGDTHVVAKGETLWSIARLYFNDPLLWPEIYRLNTATVEDPHWIYPGQVLQINGN
                     ADTTTVVAQTDTTKVVAQAPTPVDTVHADTVKADTTHAVPVDTTPKADTTAVAAPPAA
                     DTTGPPPPEETVSNNNGETIFDHPITAKQQVENQLRAYLHQVYRPVRPGEFYSAGWLT
                     EGEKLPWGEVVGNTERPAITRLSQVTVAGQYEEIAITPPGNASYHVGDSLLLARRDRD
                     VSGWGDVIVPVGIARVTTVQPKQVLGTVIQQYGRIHSGNLALPLEPFRNPGETRPAPV
                     TQGLQGAIVAQRDLEPLAGTQTIYFIDKGRSEGVVPGDIFEAYRPSTGEMGSASEQVL
                     VQFLIVHTREHSASALVIGITQPNIKAGTKVRLVRKMPS"
     gene            5329..6273
                     /locus_tag="VFU75_04255"
     CDS             5329..6273
                     /locus_tag="VFU75_04255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU5466076.1"
                     /translation="MARRVLVTGGAGFIGSHVADAHLAAGDDVTILDNLSTGRRENVP
                     ARAKLVEADIRSPEARELLAKGGFTVLNHHAAQMDVRRSVEDPVFDAGVNVVGLLNLL
                     EGARAGGVKRVVFASSGGTVYGEGDKLPFSEPDPKLPTSPYGTAKLASEYYLATFSQL
                     YGIEAAVLRYSNVYGPRQNPHGEAGVIAIFSKRIAGGEPITIFGDGKQTRDMVYALDV
                     AEANVRAATATLPRLTSLDARAWNIGTGVETTVNELAELLAQGAGKKANITRAPARAG
                     EILRSSLATDKAARELNWRPRTPLAEGLRATIRSIMEA"
     gene            6275..6988
                     /locus_tag="VFU75_04260"
     CDS             6275..6988
                     /locus_tag="VFU75_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HEU5466077.1"
                     /translation="MLLQLGRAVPTTPWELVLTSSRETKVVLVVLVLFSIASWYIIAL
                     KWWQFRRLRSQADRFMVEIEKAQRLEDAHHAAMRLQPSPYRRLLLEGIHFFSELKPGT
                     LKRDPAIPISTLTPTQLEALRMVLSKEVAAERDAAARFIPWLATFGSVSPLLGLLGTV
                     LGVMDAFIGIAAGGSGNIGAVAPGVAEALVTTVAGLAVAVPAVMAYNLFVNRLGLFAG
                     ELEGFAQEIIGTMAREGRL"
     gene            6989..7426
                     /locus_tag="VFU75_04265"
     CDS             6989..7426
                     /locus_tag="VFU75_04265"
                     /inference="COORDINATES: protein motif:HMM:NF014525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HEU5466078.1"
                     /translation="MAGGLLSGGGLRGRGELPLNADINVTSLVDVAFVLLIIFMITAP
                     IMQGGVDVRLPRAAAKPLEPNQGLIVTIDKSGSIFLDQSPLTYGEFKATFAAFVRSKH
                     PSGVYLRADQQVAYGTVVQVLAVIRESGINDVGLVAEPESVSK"
     gene            7423..8109
                     /locus_tag="VFU75_04270"
     CDS             7423..8109
                     /locus_tag="VFU75_04270"
                     /inference="COORDINATES: protein motif:HMM:NF024503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB C-terminal domain-containing protein"
                     /protein_id="HEU5466079.1"
                     /translation="MSSRSRGPSGVGIGLIGTIGVHATAVAFVIMTATRARAVTPPTY
                     AVRLVAAPDPTPDQRKVPDVVQREAATPPAPAPPTKKPAPTLKPAPAPPPNADTKKRE
                     AAPKTAPVQRIKGEAPSTGHDVATVSTEGQEFPYPEYLQNIVSQVLRRWERPDQSTPL
                     EAEVSFFVHKDGSVTGLQFTKRSGNFEFDLEAEGAVESAGQTKAFGALPDGWNQDVLF
                     VRFYFSGVKQ"
     gene            8160..9491
                     /locus_tag="VFU75_04275"
     CDS             8160..9491
                     /locus_tag="VFU75_04275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466080.1"
                     /translation="MLSLAVYPSSRLAGQDSTIDKGVRIGITYRPGVRPGLVVLPTAP
                     GALDSVHTIVGRDLDFSDRYEVITLPGGDSLDPGPSAVAKGHTAARSVAGLNYSLYQA
                     LGADFAVQFRMTGDSVLLLLHDVAAGAVRRTFSARLPAPGDPAYRMVIHRLADQVVQA
                     SLGASGTSATRVLFVKDKHVWSVDQDGANLHLVSSATKEALSPAWSQDGRKIAYMEFN
                     GGHGQINLLDLATGKRSVVYGSNGKYSDFTPTFSPDGKTIAFSRATEEGTDLWTVDYR
                     DACCLQRLTVGKFSDNLSPTFSPDGRRLAFVSTRPGLAQIYVMGADGTDQQLFAPFDY
                     GVTGSSNAPDWSPDGLSVAFHRDVGGTLQVFVLDVRKGTVKQLTSVGRNEDPTWAPDS
                     RHLAFVSDRAGYRQLWIIDLETGRIRPLLQQSGERLPAWSPRLPEAASATP"
     gene            9509..10129
                     /gene="pal"
                     /locus_tag="VFU75_04280"
     CDS             9509..10129
                     /gene="pal"
                     /locus_tag="VFU75_04280"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HEU5466081.1"
                     /translation="MTRLQSLALAALTTAIAVAACGKKPDTTPAPVVTADTAADNAKR
                     RADSIAAAQADSAAKARALADSIARARAADSAAAAARAGDEVRSMLATMIHFEFNKAE
                     IQGGDQALLDQKIAILQKNPGLRIQVAGNCDERGSQEYNLALGNRRANAAKQYLVTHG
                     IDAGRIETVSNGEERPLDPAHNEDAWAKNRRDEFTILAGGDALVKP"
     gene            10132..10905
                     /locus_tag="VFU75_04285"
     CDS             10132..10905
                     /locus_tag="VFU75_04285"
                     /inference="COORDINATES: protein motif:HMM:NF024915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5466082.1"
                     /translation="MFRRTARSLALLGLGITAGCALKGDVRKVGLEVEALQAQVTRSD
                     SLRRTERDSVLAAIAAVQQTLATQQNYLVQMRGDAKNDHLNINQQLVSVQELTGQSQQ
                     RLSELRSQIEQRAAQPVPAPDTGRGPAPSVTGASADQMYDASLQQYRKGSVATARMGF
                     RAFLQSFPTDPRASDALFYIGESFGDQQPDSAVPVYAQLVKTYPNSVRAPTALYKLGL
                     LYEKSDKAQARTYFNRVIAGYPRSDEANLARIRLQALGR"
     gene            10943..11686
                     /gene="tatC"
                     /locus_tag="VFU75_04290"
     CDS             10943..11686
                     /gene="tatC"
                     /locus_tag="VFU75_04290"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HEU5466083.1"
                     /translation="MPFLEHLEELRWRLLRSAIALTVGALIGWGVVTHFDILGILMRP
                     IAAQLPTGKLMVTGPAEGFFITMKLAVAVGLVLASPVIIYQAWAFLVPALYDRERRVI
                     VPSLMAGVLLFAAGAAAAYFLVLPRALAMLMSFQREHLEPIITADRYFAFAIPLVLAF
                     GAITELPLVMIILAGFGLVNAKFLARNRRYALVIAALIGAFLAPPDALSMILMMVPML
                     GLYEISIWCVWIVGRRRDRRAAAAAADVT"
     gene            11683..14559
                     /locus_tag="VFU75_04295"
     CDS             11683..14559
                     /locus_tag="VFU75_04295"
                     /inference="COORDINATES: protein motif:HMM:NF040057.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HEU5466084.1"
                     /translation="MRSPGAAAAIALLLTVGGAAALPGQRPAPKPTQDTTHRAGQPVQ
                     GRAIDTATARRLGLPTGPTRSFPASDALMDSLAARKGYRITHYTADSLVMRQGQRGSD
                     IQLRHDALVDQDGSKLQADSIHYVQTSCRLEAQGAPSLFGEGNVMIGDSLRYDNCLKR
                     GVITNALTSFKQGGATWFMRGDLAVDSGSTRMFGASSSITSSDLPLPDYHFQAGEVKW
                     LNKNVMIARPAVLYIRDVPLMWLPFIFQDIRLGRRSGVLVPRFGLNDLVRPTRQYQRH
                     IANLGYYWVPNDYVDLLGSLDWFSNRYVEVHGAMRYKWLDRFIDGGITYAKQSNLDGS
                     GTSTQIGWNHSQAFDSRTHLTATVNYATSAAVIQQNTINPFLSTAQLSSQANFQKQFG
                     WGTVNVGGSRSQNLSTPQVSQTFPQFSFTPSPINVTPWLTWSPGLSYTNSQTFHNNSG
                     TLLVPAGFSLIGSDTISQFFDNRTTSLGLSTPIRIGGWNWSNSLSVSDQASNQRREYF
                     VLDPADSQVHKILYVRTFQTTVDWSTGFSLPTLFTNSWKLQPSINVVNNTSQGPTAIR
                     NQFTDGAFVTQGKRLAFGAAIAPTFFGFFPGFGPLERIRHSVQLLVNYQYAPAASVSR
                     AFANAIDPSHLNPTATSDPQQTISVGLSQNIEAKLKPPPGDTAGAGRKIKLLSLSTSQ
                     IQYNFERAKQAPDILTGWQTQSLTNTMSSDLIPGFSLSLTHDLWNGPVGYRVAKFSPF
                     LTNVSASFSISEATIKGIGGLLGLGGGTAKPAAPAPGASPNAGLGGGGAGGQNVGISG
                     PGVPPPALGGGVPGISPFSGPTGGGFSMNVTFTSTRFRPVKDSSIFAPVQTDTQYQGR
                     LPVTPLPGFLPQTGAGTEQMNVTMHFSPTPHWDLNWTTTYDFHTRQFGQQFISLQRDL
                     RRWRASFQFVKSPNGNFGFNFTIQLRDEQDIKFDYDQQTFRQTQ"
     gene            14676..15836
                     /locus_tag="VFU75_04300"
     CDS             14676..15836
                     /locus_tag="VFU75_04300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466085.1"
                     /translation="MRTSLRIALTLSALIVAACDQQQTVTGGGGSPLPPPADLSYELD
                     PSGSPGQPSAILLTWQPVNSTALQSYRIYSTPTVGGTFNLRAETTSPSFHDFGPPDLQ
                     YTVTSVDLNGNESTNSNVVTITSLLTLPKPDSLFSTSLNGAVELDWTDNAYLSNPVAF
                     KWYRVYSASAPSDTLCNNDWVLEGATVSPDFIVGALTNGVSRCYFVTAIDTLGDESQW
                     SPIRDDTPRPDARNVALYPLQSNAASAGLEFWDGTANGFGLVVNGGSSSANFYVSQHA
                     DSTLWLVPVAASGTTMQQYGIGPLDDLTSIDFAPGSGYASTSLQALPGDGYVFQVVNG
                     ASVRYGAVRVTHVGRDFLILDWSFQTTAGNQELIRAGGQTVAVQTGRTIIRR"
     gene            16007..17485
                     /gene="hutH"
                     /locus_tag="VFU75_04305"
     CDS             16007..17485
                     /gene="hutH"
                     /locus_tag="VFU75_04305"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HEU5466086.1"
                     /translation="MIALDGRSLSLADVVAVARHRAPVSASPAALDRVRSSRAAVERA
                     IARGDTMYGVNTGFGKLANVRIPADKLQDLQKNLIRSHASGVGDPLPVEAVRAMMLLR
                     ANVLLMGTSGARAELPLLLVELLNRGIHPSIPSQGSVGASGDLAPLSHLALALMGEGE
                     DGAKVRAAGLTPVVLQAKEGLAFINGTQAQTGLAALLTHDAWTLWRTAHAAGAMSLEA
                     ARGTPVAFDPRIHDARPHPWQGRSAALLRELLADSAIRESHRENDPRVQDAYSLRCSP
                     QVLGAVGEGLAMAERIVTTELNSATDNPLVFGDEILSGGNFHGQPIAFALDVLGIALV
                     TLANLSERRLERVVNPDLSWGLPPFLAKNAGVESGFMTPQIVAAALVADARVLATPAS
                     VQTIPTEANQEDIVPMGMSAAWKAGRILADAERITAIELLAGAEGLEFLKPLTPGKGV
                     ARTYAAIRKLVPPLDGDRPFQADIELVAERIRAGTFDPGETA"
     gene            17482..19146
                     /gene="hutU"
                     /locus_tag="VFU75_04310"
     CDS             17482..19146
                     /gene="hutU"
                     /locus_tag="VFU75_04310"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HEU5466087.1"
                     /translation="MKGARPVRAPRGTTRTCRGWVQEAALRMLMNNLDPEVAERPDDL
                     VVYGGTGRAARDWASFDAICRTLTTLADDETLLVQSGRPVGVFRTHADAPRVLIANAN
                     LVGRWATWDVFRDLERRGLTMYGQMTAGSWIYIGTQGILQGTYETFGAVARRDFDGSL
                     AGRLVVTGGLGGMGGAQPLAATMNGGVCLAIEVDPARVKRRLETRYLDRATDSLDEAL
                     KWCEEARASRKALSVGLTANCADALPEVARRRVKVDVLTDQTSAHDTLNGYVPAGMSL
                     VDATALRARDPAGYVRAATASIVRHVEAMLALKAQGAVTFDYGNNIRTVAKDAGLDNA
                     FDIPGFVPEYIRPLFCEGKGPFRWVALSGDPKDLARTDELVLELFPKEAHLRRWITLA
                     RERVAFQGLPARICWLGQGERARFGVALNDLVAKGELSAPIVIGRDHLDTGSVASPFR
                     ETEAMRDGSDAIADWAILNALLNTASGASWVSFHHGGGVGIGNSLHAGQVIVADGTPE
                     AARRLERVLTNDPGIGVARHADAGYPEAIATAEREHVRLPMREARD"
     gene            19173..20420
                     /gene="hutI"
                     /locus_tag="VFU75_04315"
     CDS             19173..20420
                     /gene="hutI"
                     /locus_tag="VFU75_04315"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HEU5466088.1"
                     /translation="MVTCRGPARARRRDEARTVEVLPGGAVLIEGGTITAVGPYAELS
                     AAHPKATREEVRGVLYPGFIDAHTHGVFGAPRLDDHQRRALGATYQAIAAAGGGILQS
                     VRDVRGRTEDDLYRLARARLDALRAHGATTVEIKSGYGLELDAEMKQLRVIGRLKRDG
                     SPDVVATFLGAHEVPPEHRAHPDTYVATLCEDMIPAVVREHLAARCDVFCEPGVFTVA
                     ASRRILEAARSAGLALTMHADELEGAGGAELAATLGAQSADHLAAISPAGIQALARSE
                     TVAVLLPATMTFLGKKSQAPARALWEAGAAVALATDFNPGSSPTVSLPYVMALGVSQL
                     GMRHEEALTAVTVNAAAALGVAGDRGQIVPGMRADLVALDVSDWREAAYWIGANLVTA
                     VWTGGSACRSIGGPISLGGYVET"
     gene            20407..22806
                     /locus_tag="VFU75_04320"
     CDS             20407..22806
                     /locus_tag="VFU75_04320"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5466089.1"
                     /translation="MSKLEKAKERARALEVKDAKGAVDAWIEVLKEQDSSGEPNPDLA
                     IYNKVGDLWLKLKDPGQAADYYDRAVDKYAEQGLHNNAIAMCNKVLRNAPGRQNTYLK
                     LAKLYAAKGFTSEAKQNFVEYAERVHKTGNIQLAFAALKEFTDISSEGEQLRHMLEEQ
                     LAAYGVNENPQRRTTQAKVVEPPKTPGMKRKTSSLVFLDLDAPTAPKGGAAAPAAPKP
                     AAPAPKPAPAPAAKAPAVPAPPPAKAPAQTAAPKVAPPPRPTPKPAPPPPPEPEPEPV
                     SLLDEPPVAEPDTSLQIESTSLADEGISAGSGSLLEGLESTSADFGSVRDSATNIESV
                     REEIVADDVEPMAELEPTVEEEVPPPPARGTRGRQAVVELSLDEEPLVEAEPVIEAEP
                     EVLAPVDDEPLLLDDGAGQETRDFIMTEDDTSVGGRPSVHIDVGLDGGGMQIEDSAPS
                     IDFSDIEEAPTVSIEDLEGRVADNPDDPEAHQALGEALIESGDRERGIEELDHATAGF
                     ESAGNSQQAEDLVDEILRLDPNSIRHRQKVVEFAFKSGEKDRLITAYLELGDTLLRGD
                     LPDKARAVYERVAEHDPKNARAQSALSMLAPAAPAAPEGKVKAKDAKMKVRDEAAEGA
                     DFVDLGSMILDDHGEERDSRMKVQDEEPTGDEQQDFQEMLARFKQGIDENIEDTDFQS
                     HYDLGVAFKEMGLLDEAIAEFQKALRAQDGKLRTSEALGVCFFEKGAFGVAESILRRG
                     LELPSSGEEERLGILYWLGRACEEQGRPAEAKDLYGKVFAVNIRFLDVGERVKALAKA
                     R"
     gene            22808..23797
                     /locus_tag="VFU75_04325"
     CDS             22808..23797
                     /locus_tag="VFU75_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU5466090.1"
                     /translation="MSQQVAPVTLHDIQRPVAQDLVRVAEELRRIIAADFPSIAEVND
                     HLLRMKGKLFRPMLLLLSAQATGNTEPRSVSLAAVVELIHLATLVHDDSVDHSALRRG
                     LPTLNALFSHQIAVIMGDYLYSRAIIELVKAEDIEPLRVFARVTNEMTIGEMRQIEAH
                     DKLSYNEAQYDHLIRAKTASLLSGACECGALRGDPAHRAALAAFGLHLGMAFQITDDL
                     LDYTEAEAVTGKPWGLDLKEHKVTLPLIAALPRMAPEGRRLVERLMEEPEPSDHLVTD
                     VIAWVNEAGGLTYARQRALTLAQEAERALDGLPASPARDALRDSIAYAVERRS"
     gene            23801..24073
                     /locus_tag="VFU75_04330"
     CDS             23801..24073
                     /locus_tag="VFU75_04330"
                     /inference="COORDINATES: protein motif:HMM:NF025572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4321 domain-containing protein"
                     /protein_id="HEU5466091.1"
                     /translation="MARGPRRPLFYLGVLTTGFVLGGFMQALLRQFLPAGAAKEFFTT
                     AVHPTLGPVQINLLVVDITLGPIGLDVSLVAVLGVVIAYFVARSLF"
     gene            24087..24323
                     /locus_tag="VFU75_04335"
     CDS             24087..24323
                     /locus_tag="VFU75_04335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEU5466092.1"
                     /translation="MELSVWHIALLLLVFLLVFGAKRIPEIGSSLGQGIREFKRSIKE
                     IGDNPPDQRTNVPPPSATNSTLPPTGGEPKRLSQ"
     gene            24332..24547
                     /locus_tag="VFU75_04340"
     CDS             24332..24547
                     /locus_tag="VFU75_04340"
                     /inference="COORDINATES: protein motif:HMM:NF014470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HEU5466093.1"
                     /translation="MSVAGFSIGEWLVLLAVLFVAVGPRRLPEVGRAAGKFLREFRKG
                     LNEARDAMGRPDVPLPPRPAKPTSLLD"
     gene            complement(24549..26384)
                     /locus_tag="VFU75_04345"
     CDS             complement(24549..26384)
                     /locus_tag="VFU75_04345"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HEU5466094.1"
                     /translation="MMRTMRENSKYIFYVLAIAFIGWMIFDVGMNVTGVKTGGDVVLK
                     INGTEIHYPQWQAAVEAAREQYVARGGNASLTDDQQKQLEDQVVDQLTQDVMLQQSYA
                     KLGITVSDQDVIDAAKSSPPPEVMQSPDFQTNGQFDMNKWLRFLASGASPDLMVQLEA
                     RYRSVIPQQKLAEYLTADVYVPDAKLWRIWSDQHDSATVAMVAIQNVSIPDSAVKLTD
                     KDIQDYYTVHSSEFKRPAIAYTTFLVLPRAPEASDTAAALALARKVRPEVMVSESAFA
                     RVAKRQSVDTITGAKGGDLGWIKRDEPGFDAGFIAGLKNLQPGQVSQPVLSAFGYHLI
                     RVDKAAGDSVEVRHILFPIALTGPRLDLVEARADTLDRLAGEHDDGSVIDSLGKAMHL
                     NVATAPPIYQGERMTLGKYVIPDVGTWAFGGARPGQTSKVIEGERAFYVFRLDSLRPA
                     GVPPLAEIRARIDSAATFDRKKEIVRQRGQALAAKLAGASDLRQAAAAAGYAATTIGP
                     FTRFRPPAQIQLDPEVVGAAFGLKPGERSPLIEARTGDFLLQGISRSKADSTAWLAQR
                     DQQRTVLVRGLQQARVTAYLAALKAKAKVVDRRKEMFQQTQQAGS"
     gene            26537..28456
                     /locus_tag="VFU75_04350"
     CDS             26537..28456
                     /locus_tag="VFU75_04350"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5466095.1"
                     /translation="MAYTSEIEKLERRWLENPKGRNFAPLADAYRKAGEIDRAIDLCK
                     SGLELHPDYVSAHIVYGRCLIDQKDDPAATEVFQKVLQLDPENILALRILAEIAERTG
                     HYDQGAEWLGRLISADPMNGDAAEALTRMRSKAAQAKTAPRASLPAVEAPAETVAEIV
                     PEKSEPSADLPIERASSPNVPAELPPRLSLVDMATVPMNQPDFGVERASGSGKVIDAD
                     RLVDAEPELPNAAGAEAAKDQRLSLGLEAEPELPGQGAVAPRNNMGLESFDGIDFSNA
                     PPSQRMEGMMSEEGPAPNVDNVEVEGLARTQYEGSGTFRLDQPLLESTATPHLEPDGP
                     PVSLPLIMPDDVEPGTAEAPQLTPPPSPPPAPPRASRVSAPAPVPAPPLPPDDAEDVG
                     AADTATLSQAEPVLTETMAELYLKQGHKDDALRVYQALLAQRPGDARLEAKVRELDGS
                     DDAPVLTSHPMRAPASGLSLGSFLKRILGSRPGAPAADPLPEESALPGPRVTSGATPQ
                     SMLADAFADEPPLGPTPGSPSRPAADSLSLDAVFGDDGSRASAPSVEAPHEAAASAPA
                     PAAPSKGGFSFDDFFGSGGAPAPAADSPAPAAASHRPSGSAPRPSTRTSRPQAPLEDA
                     GDLDQFQAWLKGLKS"
     gene            28456..28902
                     /gene="aroQ"
                     /locus_tag="VFU75_04355"
     CDS             28456..28902
                     /gene="aroQ"
                     /locus_tag="VFU75_04355"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HEU5466096.1"
                     /translation="MRVAVLNGPNLNLLGEREPEIYGAVTLGQIEKATQERARALGVE
                     CVFSQTNHEGELVDAIQKLKGSADGALVNAAAFTHTSLAVRDALLAVRVPFVEVHLSN
                     IFAREPERRRSLLADAAVGVVAGFGADSYRLGLEGLVAHLAARRAR"
     gene            28892..29992
                     /locus_tag="VFU75_04360"
     CDS             28892..29992
                     /locus_tag="VFU75_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HEU5466097.1"
                     /translation="MPADRRPTRRQALTESLAAQGLDALLITSRANVRYLTGFSGSAG
                     MVVLTPSELLLVTDFRYDEQARDEAGSVARVEVERTSVWDRLFREMGRLGPIESVGFE
                     GHALTVREAERLVAGGKPWRWQPAGELVEALRVRKDPGEIAAIRDAATLATDALRETL
                     ARVRPGLTENEIAGVLEGALRKRGSEAHPFPTIVASGPRSALPHARSSDRQVAAGEWL
                     LIDFGATVDGYASDITRTVVVGARATEPQRALYDLVAGAQRRAREGVRAGLSGRDADA
                     LARDPITARGFGEAFGHSLGHGLGLDVHEAPRLSGTATEALPVDAVVTIEPGVYLAGE
                     GGVRIEDDVWLSAEGPVLLSDGATELLELTQD"
     gene            30026..30517
                     /gene="accB"
                     /locus_tag="VFU75_04365"
     CDS             30026..30517
                     /gene="accB"
                     /locus_tag="VFU75_04365"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEU5466098.1"
                     /translation="MNLDLISQLVKTLKDAPELGSIEIRRGFLGLWASVRVSRAGQGA
                     GGAAHVVVGNAPATAMPAAPATTAAPAAAPAPAPAPVSNLVEVKSPMVGTFYKSPEPG
                     AEAYVKVGSRVTTGQVVCIIEAMKIMNEIESEVQGVVREVCVENAQPVEFGQVLFRLD
                     PNG"
     gene            30510..31679
                     /locus_tag="VFU75_04370"
     CDS             30510..31679
                     /locus_tag="VFU75_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEU5466099.1"
                     /translation="MAETPAAGTAPAFNFKQAITQMVQRNGSDLLLKVGRPATVRVNG
                     ELVALETQPLKPEELKSLAEQIMTPRQVKEFAEHKEADFAIGVPGVGRFRTNVYQQRG
                     TVAFAFRAIPYEVKTVKDLSLPGVLEEIALKPRGLVLVTGVTGSGKSTALAALINHIN
                     LNRRVNIITIEDPIEFLHRDNLANISQREVGNDTLSFGSALRHVLRQDPDVILIGEIR
                     DMETLDTALKAADTGHLVFSTLHTTDATQSINRVISFFPPHQHDEVRHLLSTALQAVV
                     SLRLVPRKDGKGRVPAAEVLINTASVQDNIRDLTKALAIPDLIAEGSVQYGMQSFDQS
                     LFGWYQQGVVSYESALFYASNPSEFALKVSGVDSQASRAFGGSAGGGAGAIDLTP"
     gene            31679..33031
                     /gene="accC"
                     /locus_tag="VFU75_04375"
     CDS             31679..33031
                     /gene="accC"
                     /locus_tag="VFU75_04375"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU5466100.1"
                     /translation="MFKKVLIANRGEIALRVIRACKELGIETVAVYSEADRECLHVRF
                     ADDDVCIGRAPSRESYLNIPRIIAAAEITGADAIHPGYGFLAENAEFADIVRASNITF
                     IGPTPDQIRQMGDKAVARSIAQKVKIPTVPGSPGPVSSVEEGLEFGNKIGFPVIIKAA
                     AGGGGKGMRVATDPDSFTKSYNLARQEALAAFGSDQVYIEKYLARPRHVEIQVMGDTH
                     GRVMHLCERDCSIQRRHQKLVEEAPSPALNQELRLKMGQAAVTAAEAINYVGAGTVEF
                     LLDEDGSFYFMEMNTRIQVEHPVTEMCTNFDLVKEQIRVAAGESLKLKLEGTRLRGHA
                     IECRVNAEDPFRNFQPCPGHISAYHPPGGPGVRVDTHIYDGYTVPPYYDSLLAKVIVH
                     GNTREEALARMRQALDSFIIEGVTTTIPFLGRIMRHPDFIAGKVDTKFLERQPQLFKE
                     "
     gene            33028..33744
                     /locus_tag="VFU75_04380"
     CDS             33028..33744
                     /locus_tag="VFU75_04380"
                     /inference="COORDINATES: protein motif:HMM:NF015962.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HEU5466101.1"
                     /translation="MKIDVHFTPLGLSGEVPGRAVVVLDVLRSTTTIVMALANGAKAV
                     VPAASTEEAVRMTSNLQRDAYLLAGERKSLKIPGFALGNSPREMSSDAVAGKTIFLAT
                     TNGTPALVACADGGTVLVGGAINFTALADRARELFTIQSELVIVCAGREGKFALEDAY
                     TAGRIVKAVKKGIRKLDLNDAARVALGLTTEYTTWTEAFEASDAARQLIEADLGDDIA
                     FCAKADRCAVVPTYADRRIT"
     gene            33741..35972
                     /locus_tag="VFU75_04385"
     CDS             33741..35972
                     /locus_tag="VFU75_04385"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEU5466102.1"
                     /translation="MNNRAREELVGIGALVVGLFLGLTLAPWHVTGNIGSAVGDTLWK
                     AFGVGSIVIPVLGIGWALAAFGRLGSVSSPRAAILGAGLIVLLPYAVAVVKGFSAPYS
                     PDYAVWSASQRLVGLVPAFFAEVVERTTGPAGATLIGLFALSALGVFTIGWHPLAVLR
                     AGPDEETKAGKRQAKDEKRETGKVKREAGLTPMPASDVDEEAEAPIPVSRFPKDRKAK
                     PAPKVKPVAKVPPPPPPEGLIPPVELLNEPSAEAADVGIAEIDQMGQRLIQTLETFKV
                     GGRIADRTIGPVVTRFEIEPGPGVKVGRISGLADDLALSMKAQSLRILAPIPGKAAVG
                     IEVPNPHPRMVVLRELLSGDFRSDRILPVALGRNIEGEEVVADLAKMPHLLIAGQTGS
                     GKSVCINTIITSLIYAYPPERLKLLMIDPKMVELSMYGSLPHLGMQVVTNHHKAARVF
                     KWAVQEMEYRYKLLHANHARNIIDFNKKVIEKKPLKGPQETLASQEGIQKELKFEAEY
                     KDGIVPYVVLVVDELADLMLTVQGEIETPLAQLAQKARAIGIHLLLATQRPSVNVITG
                     VIKANFPSRIAFRVASKVDSRTILDQNGAESLLGNGDMLFLPPGKSEPVRVQGAFIGT
                     DETERLMDWYRGRGAERPEHSAIEAQVEAIEAQESAQAAAEGSGDPGERDPLFRQAAE
                     VCIQNQLGSTSLLQRRMSIGYGRAARIIDQLEMAGVLGPANGSKPRDVLVGLEDLDEI
                     VGA"
     gene            36082..36474
                     /locus_tag="VFU75_04390"
     CDS             36082..36474
                     /locus_tag="VFU75_04390"
                     /inference="COORDINATES: protein motif:HMM:NF014119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HEU5466103.1"
                     /translation="MSSGVRSDPETWEDPRHREGLAGELQAIDYLKTQGWVLIAHRWR
                     VGRIEIDLVVRRGKLVAFVEVKTRRGDAFGHPFEAVTGAKRREIVKAARAWTDRHGAP
                     DDIYRFDCIGIQNNKLTHLEDAFRPGWR"
     gene            36532..37626
                     /gene="recA"
                     /locus_tag="VFU75_04395"
     CDS             36532..37626
                     /gene="recA"
                     /locus_tag="VFU75_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEU5466104.1"
                     /translation="MTRTPVAADPQENPKLSDDRRKALNLAITQIEKSIGKGAIMRMG
                     AEARVRVEAIPTGAINLDAAIGIGGIPRGRVTEIFGPESSGKTTLALHVVARAQKGGG
                     AAAYIDAEHALDVEYAKKLGVDIDNLLVSQPDTGEQALEICEILVRSGAVDVVVVDSV
                     AALVPKAEIEGEMGDSHMGLQARLMSQALRKLAGAINRSRTAVIFINQLREKIGVMFG
                     NPETTTGGRALKFYASLRLDVRRIGPVKERETVIGNHVRVKVVKNKVAPPFRQAEFDL
                     MFDEGISHESLLVDIAAEAGIIEKAGAWYAYGKERIGQGRENAKMYLKDHPEMALEIE
                     LKVKEVLGMKSSAAGSTETATDEEAPAAEE"
BASE COUNT         6349 a        12590 c        12797 g         5978 t
ORIGIN      
        1 ctactaccag cccgaagcgt acgtcccgca gaccgacacc tacatcgcga aagactcgtc
       61 gatcaacgac gacatcgacc gcctgcgcct gcgggcgacc tcgagcttga tcgagcggga
      121 ggacgtgatc gtggtggcga gcgtctccgc catctacggc ctgggagacc cggagctcta
      181 caaacagctc atggtcacgg tcgacgtggg cgccgagcgc ggccgggacg cgattctgga
      241 agacctggtg cgcatccagt acacccggaa cgacgtcaac ctcgaacggg gtgtgttccg
      301 ggtgcgcggc gacaccatcg aagtcttccc ggcgtacgag gagcagggtg tccggatcga
      361 gctgtgggga gacacggtgg aacgggtgac gaagttcgat cccgtcaccg ggaatgccat
      421 cgtcgatctc aaacgtgcgg ccatctaccc ggccacgcac ttcgtcactc agcgtccctc
      481 cgtcgagcgc gccgtcgcgt ccatccgggc cgagctgaag gaccggctcg ccgagctgcg
      541 ggtgcagaac aagctggtgg aggcccagcg gctcgaagcc cggacgacgt tcgacgtcga
      601 catgctcctc gaagtgggcc ggtgctccgg aatcgagaat tactcgcggc atctccgggg
      661 cagtgcgccc ggcgagcgcc cggcgtgtct gctcgactac ttcccgagcg acttcctctg
      721 cgtgatcgac gagtcccatc aaaccctccc ccagatcggc gggatgtacg agggagaccg
      781 gtcgcgcaag cagacgctcg tggattacgg attccggctg ccgtcggccc tggacaaccg
      841 cccactcaag tacgacgagt tcttccggct cacgcccagg atggtcttcg tgtccgccac
      901 tcccggcgaa gaggagctca agctgtcggg tggcgtggtg gtcgagcaga tcatcaggcc
      961 gaccgggctc atcgatcccg aagtcgagat ccggccggtc aaggggcagg tcgacgatct
     1021 gctgcacgaa gtgcgggagc gggcgaaggt gagcgagcgg gtgctcgtca ccaccctcac
     1081 caagcggatg gcggaggacc tgacggacta cctgcagcag caggcggtgc gggtgcggta
     1141 tctgcactcc gagatcgacg ccatcgagcg cgtcgagatc atccgcgggc tgcggctcgg
     1201 cgagttcgac gtgctcgtgg ggatcaacct gctgcgcgag gggctggatt tgcccgaggt
     1261 gtccctggtg gccgtgctgg atgccgatca ggagggattc ctgcgctccg accggtccct
     1321 gattcagacg gtgggacggg cggctcgaaa cgttcacggc cgcgcgattc tctatgcgga
     1381 ccggatgacg ggttcgatgc aacgggcgct ggacgagatg agccgccgcc gcaccatcca
     1441 gcaggcctac aatgtggagc atggcgtcac gccgacgtcg atcgtgaagt ccgtgttgga
     1501 tgtccggttc gtcacccgcg tggccgatgc gcgcgtgggc cgtcccgaaa cgggaggcgc
     1561 gaagaaaccg gcggatgccc gcgagcgcga ggccctgatc gcgctgctgg aatcccagat
     1621 gcaggccgcc gccgaacagc tcgacttcga agcggcggcc ctgctgcgcg accaaatcct
     1681 cgatctgaag gcggagagcg atgccgggcg ccgtgcgcct caccgcggac gagctcaacc
     1741 ggttcggtga ggagctgggg cgcacgctcc aggctcccgc ggtcctcggg ttctcgggcg
     1801 agctcggcgc cgggaagacg accctggtcc aggcgatctg ccgcggcttg aaggcggagt
     1861 cgcccgcgac cagtcccacg tacgccctcg tgcaccgtta cctggcaccg ggaggtcccg
     1921 tgtaccacgt ggacggctac cggctcaagg cgcctgcgga cgcgcgtgac ctggggttcg
     1981 aggagatgga gcgcgaaggg gccatcgtgc tgatcgagtg gccggagcgc gccctgggct
     2041 gggcgcccgc gctcgaccgg catttccacc tggcgcatga cgcggatccg gatcgccggt
     2101 ccctggagga ggagtgagcg ccctgatgct ggcggtcgac accgcgaccg acatcgcgag
     2161 cgtggcggtt ggggacggct ccggcgtcaa ttccggggca caccagcagg gctcgcgccg
     2221 tcacgcggcg gagatcgtgc ggctgatcga cttcgcgctg tcccgcgccg gcgcacagat
     2281 cgcggatctc gacggcgtcg tcgtggggga cggaccgggg tcgttcaccg gactccggat
     2341 cggctgggcc gccgccaagg ggctggtgca ggagtcgggg gccgacgtca tcgccgtgcc
     2401 gtcgctcatg gccgcggccg cgggcgccgc ggagcgggtg ggcagcgtgc cggtagccgc
     2461 ctgcttcgac gcgctccggg gccaggtgta cggagcgctg tacgtcgttc acgccgaccg
     2521 cgtcgacacc atcgtgccgc cgacgctcgc gacgatcgcg gaactcgaag ggctggcggg
     2581 cgcgcgacgt cccgcgctcg ccgtcggtga tggcgccgtg cggttcgggg ccgaagtgac
     2641 gaaatggacg ggcgtgccgc ccgtcccgct cgaccagctc cccccctccg cggtcgtgat
     2701 gctggcactc gcgggccggc ccggagcggg acgacggatc gaggacttcg ggaccgccga
     2761 gcccgagtat ggccggccgg cggaggccca ggtgaaatgg gaggcccgac acggacgcca
     2821 gcttccgcgt tcgaccgacg ctccccgctg acctccccgc gattcagggg atcgaggcgg
     2881 tcagcttttc cgacccctgg tccgcgcggg attttctcga gacgctcgcg accggcgtca
     2941 tttttcttgc ggccgtcgac gatggcggag gggtgctcgg gtatctcgtc gcccggcaaa
     3001 tggcggacga aggggaaatt ctgaatgtgg gagtcgatcc ccagcgccgc cggatcggcg
     3061 tcggtcgcgc cctcgttggt gccgccctgg cccggctccg atccctcggc gcgcggcagg
     3121 tctacctcga ggtgcgggag tcgaacctcg cggcgcgcgc gttgtaccgt ggatttgggt
     3181 tcgccgaggt ctcaagacgg gtgaagtact accggcggcc ggtggaggac gcgatcctcc
     3241 tgcgtgccgc cattccggca ggggagggcc cccgataaaa tggtgaaaag tggcgtatct
     3301 ttgattgaca gaataacttg cgattcatat actgtgcggg aatccttggg accagcccgc
     3361 cttaagtacc ggaggtcgtt gtgagccgca gtctgaataa agtcatactg attgggaact
     3421 taggggctga cccagaggtc cggacgacga acaatggctc caaggtcgcg acggtgtcgc
     3481 tggccacagg acggcagtgg actggccagg gcggggacaa gcaggaaaag acggagtggc
     3541 accgggtcgt gatgtggaat aacaagaccg gcaacggtct cgcggacttg gccgagaagt
     3601 tcatgaagaa gggtgaccgg atctacgtcg agggacggat cgagtaccgg acctgggaag
     3661 acaaagagaa acagacccgg tatacgacgg agatcatcgc gcgtgaagtg atgctgctct
     3721 cgccccgtgg tggtgggggg gagggtggcg atttctcacg ggctcccgcc aaggcgaccg
     3781 ccaagggggg agctgagggg aaaccggagt cgctcgactc gttcccggag gcgttggatg
     3841 cggaggatga cgatctcccg ttctagccga acgacacgga cggcggtagc cgccgtggcc
     3901 gtcctgttgg ccgtcccgct cctgggcgcg cggctcagcg cccaggacac gacgaaggcc
     3961 aagccggcac ccaccgacac cacgaaggcg aaggccccgg ccgacacggc ttcgagcctc
     4021 ctgcagcagg cggcgacggg ggacacgcac gtggtggcca agggcgagac cctctggtcc
     4081 atcgcccgcc tgtatttcaa cgacccgctg ctctggccgg agatctaccg tctgaacacc
     4141 gcgacggtgg aggatccgca ctggatctat cccggccagg tgctgcagat caacggcaac
     4201 gccgatacga ccacggtggt cgcgcagact gacaccacga aggtggtggc gcaggcgccg
     4261 acgccggtcg acaccgtgca cgccgacacc gtgaaggcgg acaccacgca cgccgtcccg
     4321 gtggatacga cacccaaggc cgataccacc gccgtcgccg cgcctccggc cgccgatacc
     4381 accgggcctc cgccgcccga ggagaccgtc tcgaacaaca atggcgagac gattttcgac
     4441 caccccatca cggccaagca gcaggtcgag aatcagctgc gcgcctatct gcaccaggtc
     4501 taccgtccgg tccgccccgg cgagttctac tcggccggct ggttgaccga gggcgagaag
     4561 cttccctggg gtgaggtcgt gggcaacacg gaacggccgg ccatcacccg gctgtctcag
     4621 gtgacggtgg ccggtcaata cgaggaaatc gcaattaccc cgccgggaaa cgccagctat
     4681 cacgtcggtg actcgctgct actggcgcgg cgcgaccgcg acgtttcggg gtggggagac
     4741 gtcatcgttc ccgtcggcat cgcgcgcgtg accaccgtcc agccgaagca ggtgctgggc
     4801 acggtgatcc agcaatacgg ccgcattcac agcggcaacc tcgcgttgcc tctcgaaccg
     4861 ttccggaatc cgggtgagac ccggccggcg ccggtgacgc agggtctcca gggcgcgata
     4921 gttgcacagc gggacctcga gcccctggcg gggacgcaga cgatttactt catcgacaag
     4981 ggacgctccg aaggcgtggt cccgggcgac atcttcgaag cctaccgccc ctcgacgggc
     5041 gaaatgggca gcgcgtcgga gcaggtgttg gtgcagttcc tgatcgtgca cacccgagag
     5101 cactccgctt cggccctggt gatcggcatt acgcagccca acatcaaggc cgggaccaag
     5161 gttcgcctgg tccggaagat gccctcctga tccgtcgccg gaccgcgtca attgggtccg
     5221 gcgtcgtctc gccagtcctg agtattccgt gtgccccgcc atgcggcgcg atatcgcgcg
     5281 ccggggccgg ggcacattcg gactccaact agggaaacgc gctcgactat ggctcgaagg
     5341 gttctggtga cgggtggtgc gggcttcatc ggctctcacg tggcggacgc ccatctcgca
     5401 gcgggcgatg acgtcacgat cctcgacaat ctctcgaccg gacggcggga gaacgtgccc
     5461 gccagggcga agctggtgga agccgatatc cggtctcccg aggctcggga gcttctcgcg
     5521 aagggcggct tcacggtcct gaaccatcac gccgcgcaga tggacgtccg gcgctcggta
     5581 gaggatccgg tcttcgacgc cggcgtgaac gtcgtgggtc tgctcaacct cctcgaagga
     5641 gcccgggcgg gtggggtgaa gcgggtggtc ttcgcgtcgt ccggaggtac ggtctatggc
     5701 gaaggagaca aactgccatt ctccgagccc gatccgaaac tgcccacgtc tccctatggg
     5761 accgccaagc tcgcttccga gtactatctg gcgacgtttt cccagctgta cgggatcgag
     5821 gccgccgtcc tgcggtacag caacgtgtac gggccccggc agaatcctca cggcgaagcg
     5881 ggcgtgatcg cgattttctc gaaacgcatc gccggcggag aacccattac cattttcggt
     5941 gatggcaagc agacgcggga catggtgtat gccctggacg tggccgaggc caacgtccgg
     6001 gccgcgacgg cgaccttgcc gcggctgacg agtctcgacg cccgggcctg gaatatcggg
     6061 accggcgtgg agaccaccgt gaatgagctg gcggaactgc tggcgcaagg cgccggcaag
     6121 aaggcgaaca tcacgcgggc accggcccgg gccggggaga ttctccgcag ctcgctcgcg
     6181 acggacaagg ccgcccggga gctgaactgg cgtccccgca cgccgctggc ggagggcctg
     6241 cgggcgacga ttcgctcaat aatggaggcg tgacgtgctg ctgcagctcg ggagggccgt
     6301 ccccactaca ccgtgggagc tggtactcac ctcgagtcgt gagacgaagg tcgtcctcgt
     6361 ggtcctcgtc ctcttctcaa tcgcgtcgtg gtacatcatc gcgctcaagt ggtggcagtt
     6421 ccgccggctc cgcagtcagg cggatcgctt catggtcgaa atcgagaaag cccagcggct
     6481 cgaagacgcc catcatgccg ccatgcggct ccagccctct ccctatcgcc gcctgctgct
     6541 cgagggcatc cacttctttt cggagctgaa gccgggcacc ctcaagcggg atccggcgat
     6601 tccgatctcg acgctcacgc cgacgcagct cgaagcgctg cggatggtgc tgtcgaagga
     6661 agtcgccgcg gaacgggatg cggccgcccg gttcattccg tggctcgcga cgttcgggtc
     6721 cgtctccccg ctgctcggtc tcctgggcac cgtcctcggc gtgatggacg cgttcattgg
     6781 catcgccgcc ggcgggtccg ggaacatcgg cgcggtcgcg ccgggcgtcg cggaagcgct
     6841 cgtgaccacg gtggcgggtc tcgcggtcgc cgtgcccgcg gtgatggcgt acaacctgtt
     6901 cgtgaaccgg ttgggattgt tcgcgggcga gctggagggt ttcgcgcagg agatcatcgg
     6961 gaccatggcc cgggaagggc ggctctagat ggccggcggc ctcctctcgg ggggcggtct
     7021 ccgcggacgc ggagaacttc ctctcaacgc cgacatcaac gtgacgtcgc tcgtggacgt
     7081 cgcgttcgtg ctgctgatca tcttcatgat tacggcgccc atcatgcagg gcggggtgga
     7141 cgtccggctc ccccgggccg ccgcgaagcc gctcgaaccc aaccaggggc tcatcgtcac
     7201 gatcgacaag tcgggctcga tcttcctgga ccagtccccc ctcacgtacg gggaattcaa
     7261 ggccaccttc gccgcgttcg tgcggagcaa acatccgtcc ggcgtgtacc tccgggcgga
     7321 ccagcaggtc gcctatggaa cggtggtgca ggtactggcc gtgatcaggg aatcgggaat
     7381 caacgacgtc ggtctcgtgg ccgaaccgga gtccgtgagc aagtgagcag ccggtcccgt
     7441 ggaccgagtg gcgtcggcat cggcctgatc gggacgatcg gggtgcacgc caccgccgtg
     7501 gccttcgtca tcatgacggc cacccgcgcg cgtgcggtga ccccgccgac gtacgcggtg
     7561 cggctcgtgg cggcgcccga tcccacgccc gatcagcgga aggtgccgga cgtcgtgcag
     7621 cgcgaggcgg cgacgccgcc cgcgccggcg ccgccgacga agaagcccgc gcccacgctg
     7681 aagccggctc cggcgccgcc gccgaacgcc gacaccaaga agcgcgaggc ggcgcccaag
     7741 accgcaccgg tccagcggat caagggggaa gcgccgtcga cgggacacga cgtcgcgacg
     7801 gtcagcaccg aagggcagga gtttccgtac ccggagtatc tgcagaacat cgtgtcgcag
     7861 gtgctcaggc gctgggagcg gcccgaccaa agcacgccgc tcgaagctga ggtgtcattc
     7921 ttcgtgcata aggatggatc ggtgacggga ctgcagttca ccaagcggtc gggcaacttc
     7981 gagttcgatc tcgaggcgga gggggccgtc gagtcggcgg ggcagacgaa ggccttcggc
     8041 gcgctgccgg acggctggaa tcaggatgtg ctgttcgtcc ggttctactt ctccggagtg
     8101 aagcagtgag aacgtcgaga cggctagacg gttggacggc tagtcgccgg tttgcgctct
     8161 tgctgtcact agccgtctac ccgtctagcc gtctagctgg tcaggactcg acgatcgaca
     8221 aaggggtccg tatcggcatc acctaccggc ccggggtccg ccccggcctg gtggtgcttc
     8281 ccaccgcacc cggggccctc gactcggttc acaccatcgt gggtcgcgat ctcgattttt
     8341 cggaccgcta cgaggtcatc accttgccgg ggggcgattc actcgatccg ggaccatccg
     8401 cggtcgccaa gggccacacc gcggccagga gcgtcgcggg gctcaactac tcgctgtacc
     8461 aggcgctcgg ggccgacttc gcggtgcagt tccggatgac cggcgactcc gtcctgttgc
     8521 ttctgcacga cgttgcggcg ggggcggtgc gccggacctt cagcgcgcgc ctgccggccc
     8581 cgggtgatcc ggcgtaccgg atggtgatcc accgtctggc cgatcaggtc gttcaggcct
     8641 ccctgggcgc ctccggcacg tccgccacgc gggttctgtt cgtgaaggac aagcacgtgt
     8701 ggtccgtgga ccaggatggc gccaacctgc atctggtgtc gtcggccacg aaggaggccc
     8761 tctcccccgc ctggtcccag gacggccgga agatcgcgta catggagttc aacggggggc
     8821 atgggcagat caatctgctc gacctcgcca ccggaaagcg ttcggtggtg tacggaagca
     8881 acggaaagta ctcggatttt acgcccactt tctcgcctga cggcaagacc atcgcgttca
     8941 gccgggcgac cgaagaggga accgatttat ggaccgtgga ttatcgcgat gcctgctgtc
     9001 tgcagcgctt gaccgtagga aagttttctg acaacttgtc cccgaccttc agtccggacg
     9061 gacgtcgtct cgccttcgtc tcgacccgcc cgggtctggc gcagatttac gtgatgggcg
     9121 ccgacgggac cgatcaacag ctgttcgctc ccttcgacta tggtgtaacc gggtcctcca
     9181 atgcgcccga ttggtccccg gatggactct cggtagcttt tcaccgtgac gtcggcggca
     9241 ccttgcaggt ctttgtgctg gacgtccgca agggtacggt gaagcagttg acgtcggtcg
     9301 ggcgcaacga agatccgacg tgggcacccg acagccgcca tttggcgttc gtttcggatc
     9361 gcgcggggta ccggcagctc tggatcatcg atttggagac cggccgcatc cgtcccctgt
     9421 tgcagcagag cggggaacgt ctgccggcct ggtcgccgcg gctgccggaa gccgcctcag
     9481 ccaccccctg acctggagac ccaagtccat gacacgcctg caatcgctcg cactggcggc
     9541 actcacgacg gcaatcgccg tcgccgcctg cggcaagaag cccgacacca ccccggcccc
     9601 ggtcgtcacg gcggacaccg ccgctgacaa cgccaagcgc cgcgccgact ccatcgccgc
     9661 cgcgcaggcc gattctgccg cgaaggcgcg tgcgctggcc gacagcatcg cgcgggcccg
     9721 cgcggcggat tccgccgcgg ctgcggcgcg tgccggagac gaggtgcggt cgatgctggc
     9781 gacgatgatc cacttcgagt tcaacaaggc cgagatccag ggcggtgatc aggcgctcct
     9841 cgatcagaag atcgcgatcc tccagaagaa ccccggcctg cggatccagg tcgccgggaa
     9901 ttgcgacgag cgcggctcgc aggagtacaa cctcgcgctc ggcaaccgcc gcgccaacgc
     9961 cgccaagcag tacctggtga cgcacggcat cgacgcgggc cgtatcgaaa ccgtgtcgaa
    10021 cggcgaagag cggccgctcg atcccgctca caacgaagac gcctgggcca agaaccgtcg
    10081 cgatgagttc accatcctcg ccggtggcga cgccctggtg aagccgtaag ggtgttccgc
    10141 agaacagcgc gatccctcgc gctcctcggc ctcgggatca ccgccggatg cgccctcaaa
    10201 ggcgacgtcc ggaaggtcgg tctcgaggtc gaggcccttc aggcgcaggt gacccgttcc
    10261 gattcgttgc ggcgcacgga acgggacagc gtgctcgcgg ccatcgccgc cgtgcagcag
    10321 acgctcgcca cgcagcagaa ctatctggtg cagatgcggg gcgacgcgaa gaacgatcac
    10381 ctcaacatca atcaacagct ggtcagcgtg caggagctca ccgggcagag ccagcagcgg
    10441 ctgtccgagc tccgctcgca gatcgagcaa cgggccgcgc aaccggtgcc cgcacccgat
    10501 acgggccggg gtcccgcgcc gagcgtgacc ggcgccagcg cggaccagat gtacgacgct
    10561 tcgttgcagc aataccggaa gggcagtgtg gcgacggcgc ggatgggatt ccgcgccttc
    10621 ctgcagtcgt tcccgacgga cccgcgcgcc tcggacgccc tcttctacat cggagagagc
    10681 ttcggcgatc agcaacccga ttccgcggtg ccggtgtatg cacagctcgt gaagacctac
    10741 cccaattcgg tccgggcacc cacggcgctc tacaaactcg ggctcctgta cgagaagtcg
    10801 gacaaggcgc aggctcggac gtacttcaac cgggtgatcg cgggctatcc ccgttcggac
    10861 gaggccaacc tcgcccgcat ccggctccag gcactcgggc gctagcccgg cccccgtggc
    10921 cgaatcacga tcccgcggcg aaatgccgtt cctcgagcac ctcgaggagc tccggtggcg
    10981 cctcctccgg agcgcgatcg cgctgacggt cggtgccctg atcggctggg gcgtcgtcac
    11041 ccacttcgac atcctcggca tcctgatgcg cccgatcgcg gcgcagctgc ccaccggcaa
    11101 gctcatggtg accggacccg ccgaagggtt cttcatcacc atgaaattgg ccgtggcggt
    11161 gggactcgtg ctcgcctccc cggtcatcat ctatcaggcc tgggccttcc tggtgcccgc
    11221 gctgtacgac cgcgagcggc gcgtcatcgt gccgtccctc atggcgggcg tgctgctctt
    11281 cgccgccggt gccgcggccg cctatttcct ggtgctgccc cgggccctcg ccatgctcat
    11341 gtcgttccag cgggagcacc tggagcccat catcacggcg gaccgctact ttgcgttcgc
    11401 catcccgctg gtgctggcgt tcggcgccat taccgagctg ccgctggtga tgatcatcct
    11461 cgccggtttc gggctcgtga acgcgaagtt cctggcgcgg aaccgccggt acgcgctcgt
    11521 gatcgcggcg ttgatcggcg ccttcctcgc cccccccgac gcgctttcga tgatcctgat
    11581 gatggtgccg atgctgggac tgtatgagat cagcatctgg tgtgtctgga tcgtgggccg
    11641 gcgacgggac cggcgcgccg ccgccgcggc tgcggatgtg acgtgaggag tccgggagct
    11701 gcggccgcca tcgcgctgct cctgaccgtc ggtggagccg ccgcgttgcc gggccagcgg
    11761 ccggctccca agcccacgca ggacaccacc caccgggccg gccagccggt gcagggccgc
    11821 gccatcgaca ccgccacggc gcgccgtctg ggactgccga ccggccccac gcgttccttc
    11881 cccgcctccg atgccctgat ggactcgctg gccgcccgga agggataccg gatcacgcac
    11941 tacacggccg attcactggt gatgcggcag ggacagcggg ggagcgatat ccagctgcgt
    12001 cacgacgcgc tggtcgacca ggatggcagc aagctgcagg cggactcgat tcactacgtc
    12061 cagaccagct gccggctcga ggcccaggga gcgccgtcgt tgttcgggga agggaacgtc
    12121 atgatcggcg attccctgcg gtacgacaac tgcctgaagc gcggtgtgat tacgaatgcc
    12181 ctgacgagct tcaaacaggg cggggcgacc tggttcatgc gcggagacct cgccgtcgac
    12241 tcggggtcca cccgcatgtt cggcgcgtcg tcgtcgatca cgtcatccga ccttccgctc
    12301 cccgactacc acttccaggc cggtgaagtg aagtggctga acaagaacgt gatgatcgcg
    12361 cgccccgccg tgctgtacat ccgggacgtc cccttgatgt ggctgccctt catcttccag
    12421 gacatccggt tgggacggcg gagcggcgtg ctggtgccgc ggttcggcct caatgacctg
    12481 gtgcgtccca cccggcagta ccagcggcat atcgcgaacc tgggctacta ctgggtcccc
    12541 aacgactacg tcgatctgct gggatcgctg gactggttct cgaatcgcta cgtcgaagtc
    12601 cacggggcca tgcgctacaa gtggctggac cgcttcatcg acggcggcat cacgtacgcc
    12661 aagcagagca acctggacgg gtccggcacc tcgacgcaga tcggctggaa ccactcacag
    12721 gcgttcgaca gccggacgca cctgacggcg accgtcaatt acgccaccag cgcggcggtc
    12781 atccagcaga acaccatcaa tccgtttctc tcgacggccc agctgtcctc ccaggcgaac
    12841 ttccagaagc agttcggctg gggtaccgtg aacgtcggcg ggtcgcggtc gcagaacctc
    12901 tcgacgccgc aggtgagtca gacgttcccg cagttcagct tcacgccgtc gccgatcaac
    12961 gtcactccgt ggctgacctg gtcgccgggg ctcagctaca ccaattcgca gacgttccac
    13021 aacaactccg gcaccctgct ggtccccgcc ggtttcagcc tgatcggatc cgacaccatc
    13081 tcccagttct tcgacaaccg gacgacgtca ttgggattgt cgacgccgat ccggatcggc
    13141 ggctggaact ggtcgaattc gctgagcgtg agtgatcagg cgtcgaatca gcggcgcgaa
    13201 tacttcgtgc tcgaccctgc cgactcgcag gtccacaaga ttctgtacgt ccggaccttc
    13261 cagaccacgg tggactggtc gacgggattc agcctgccca cgctcttcac gaattcgtgg
    13321 aagctgcagc ccagtatcaa cgtggtgaac aatacgtcgc agggacccac cgccattcgc
    13381 aaccagttca ccgacggcgc gttcgtcacc caggggaagc ggctggcgtt cggcgcggcg
    13441 atcgcgccga cgttcttcgg attcttcccc gggttcggac cgctcgaacg catccggcac
    13501 tccgtccagt tgctggtgaa ctatcagtac gcacccgcgg cgtccgtctc ccgggcattc
    13561 gcgaacgcca tcgaccccag ccacctgaac cccacggcga cctccgatcc gcagcagacg
    13621 atcagcgtgg ggctctcgca gaacatcgaa gccaagctga agccgccccc gggcgatacg
    13681 gcgggggcgg ggcgaaagat caaactgctc tcgctcagta cgtcccagat tcaatacaac
    13741 ttcgagcggg ccaagcaggc accggacatc ctcaccggct ggcagaccca gtcgctgacc
    13801 aacaccatgt cgtcggacct gattccggga ttttccctgt ccttgaccca cgatctctgg
    13861 aacggcccgg tcggctacag ggtcgcgaag ttctctccct tcctcaccaa cgtctcggcc
    13921 tccttctcga tttccgaggc gaccatcaag ggcatcgggg gcttgctcgg actgggcggc
    13981 gggaccgcga agccggcggc ccccgcgccc ggcgcgtcgc cgaatgccgg actcggaggg
    14041 ggaggggccg gcgggcagaa cgtcggcatc tcgggccccg gcgttccgcc cccggccttg
    14101 ggaggaggcg tccccggaat ctccccgttc tccggtccga ccggcggggg cttttcgatg
    14161 aacgtgacct tcacgagcac gcgattccgg ccggtcaagg actccagcat cttcgctccc
    14221 gtccagaccg acacgcagta ccaagggcgg ctgccggtca cgcctctccc ggggtttctg
    14281 ccgcagaccg gtgcgggtac ggaacagatg aacgtgacga tgcacttcag ccccacgcct
    14341 cactgggacc tgaactggac gacgacctac gactttcaca cgcgccagtt cggccagcag
    14401 ttcatctccc tccagcggga tctgcgccgc tggcgggcga gcttccagtt cgtgaagagc
    14461 ccgaatggga atttcggatt caacttcacc atccagctcc gcgacgagca ggacatcaag
    14521 ttcgactacg accagcagac cttccggcag acgcagtaac ctgagtctct gtcctcgccg
    14581 tgggacaggt tccgcgggaa acccgcatcc cgcctcgcgg aacaccacgg cacggtccct
    14641 gccttgtaca gggccgacct ctatcggggc ctgatatgcg cacgtctttg cggatcgcgc
    14701 tgacgctgtc ggcgctcatc gttgcagcct gcgaccagca gcagaccgtc accggtggcg
    14761 gcggatcacc gctgcctccg ccggcggatc tgtcgtacga gctcgatccc tccgggtctc
    14821 cgggccagcc gtcggccatt ctgctgacct ggcagccggt caactcgacg gcgctgcaga
    14881 gctaccggat ctactcgacg ccgacggtcg ggggcacgtt caacctgcgc gccgaaacga
    14941 cgtcgccgtc cttccacgac ttcggccccc cggacctcca gtacaccgtc acgtcggtgg
    15001 acctgaacgg gaacgagagc acgaacagca acgtcgtgac catcacgtcg ctcctgaccc
    15061 tgccgaagcc cgacagcctg ttctcgactt cgctcaacgg cgcggtggag ctcgactgga
    15121 ccgacaatgc ctacctcagc aatccggtgg cgttcaagtg gtaccgcgtc tattccgcca
    15181 gcgcgccgtc cgacaccctg tgcaacaacg actgggtgct ggaaggcgcg acggtgtccc
    15241 ccgatttcat cgtcggcgct ttgaccaacg gcgtgtcgcg ctgctacttc gtgacggcga
    15301 tcgacaccct cggtgacgaa agccaatggt caccgatccg cgacgacacg ccgcgccccg
    15361 acgcccgcaa cgtcgcgttg tatcccctgc agtcgaacgc ggcgtcggcc ggactcgagt
    15421 tctgggacgg cacggccaac ggcttcgggc tcgtcgtgaa tggcggatcg tcgagcgcga
    15481 acttctacgt gtcccagcac gccgattcga ccctctggct cgtccccgta gccgcctcgg
    15541 gaaccaccat gcagcagtat ggcatcgggc cactcgatga cctgacgtcg atcgatttcg
    15601 caccgggttc gggctatgcg agcacgtcgc tccaggccct cccgggtgac gggtacgttt
    15661 tccaggtcgt gaatggagcg tcggtcaggt acggcgccgt ccgggtgacc catgtcggac
    15721 gggatttcct gattctcgat tggagtttcc agacgacggc cggaaaccag gagctcatca
    15781 gggcgggtgg acagacggtc gccgtgcaga cgggcaggac tattatcagg cggtaggatt
    15841 cagacaggcg cccaggcgct caggcgccga gcggtaggca ggggtcagta tcagggcagt
    15901 ctctcctgat cctgccccct gttgccttct cggcgcctaa gcgcctaggc gcctcacctt
    15961 gcgcctgact ttgcttgtcc ctctccgcct actagcttct cgtgccatga tcgccctaga
    16021 cggtcgttcc ctctccctcg ctgatgtcgt cgccgtcgcc cggcaccggg ctcctgtctc
    16081 cgcttctccg gcggccctgg atcgcgttcg gtcctcccgc gccgcggtcg agcgtgccat
    16141 cgcgcgcggg gacacgatgt acggcgtgaa caccggcttc ggaaagctcg ccaacgtccg
    16201 cattccggcg gacaagctgc aggatctcca gaaaaatctc attcggagtc atgcctccgg
    16261 cgtcggcgat ccgttgccgg tggaggcggt gcgcgcgatg atgctgctgc gcgccaacgt
    16321 cctcctgatg ggcaccagcg gcgcgcgggc cgagttgccg ctgctcctgg tcgagctcct
    16381 gaaccgcggc atccatccct ccatcccctc gcaggggagc gtgggagcgt cgggtgacct
    16441 ggccccgctg tcacacctcg cgctcgcgct gatgggcgaa ggcgaggacg gggcgaaggt
    16501 ccgcgccgcg ggtctcaccc cggtcgtcct ccaggccaag gaaggtctcg ccttcatcaa
    16561 cgggacccag gcccagacgg gactggcggc cctcctgacc cacgacgcgt ggacgctctg
    16621 gcggaccgcg cacgccgccg gcgccatgag cctcgaggcg gcgcggggaa cgcccgtcgc
    16681 gttcgatccc cgcatccatg atgcccgtcc gcacccgtgg cagggccggt cggcggcgct
    16741 cctccgtgag ctcctcgcgg actcggccat ccgggagtcg catcgcgaga atgacccccg
    16801 cgtgcaggat gcctactcgc tccgctgctc tccccaagtc ctgggcgcgg tcggcgaggg
    16861 gctcgcgatg gccgagcgga tcgtcaccac ggagctcaac tcggccaccg acaatcccct
    16921 ggtcttcggc gacgagattc tttccggcgg caatttccat ggccagccga tcgcctttgc
    16981 gctcgacgtc ctgggtatcg cgctcgtgac gctcgcgaat ctctccgagc ggcggctcga
    17041 gcgggtggtc aatcccgacc tctcctgggg cctgcccccg tttctcgcga agaacgcggg
    17101 cgtggagtcg ggcttcatga cgccgcagat cgtcgccgcc gcgctcgtgg ccgacgcccg
    17161 ggtgctcgcg acgcccgcgt ccgtgcagac cattccgacg gaagcgaatc aggaagacat
    17221 cgtgccgatg gggatgagcg ccgcgtggaa ggcggggcgg atcctggccg acgcggaacg
    17281 cattaccgcg atcgagctcc tggcgggggc cgagggactc gagtttctga agccgctgac
    17341 cccggggaag ggggtcgccc gtacctacgc cgcgatccgg aagctcgtgc cgccactcga
    17401 cggagaccgg cccttccagg cggacatcga gctggtggcg gagcggatcc gcgcgggcac
    17461 gttcgatccc ggcgagaccg catgaaggga gcgcgcccgg tgcgggctcc tcgcggaacc
    17521 acccggacct gccgcggctg ggtgcaggaa gcggcgctcc gcatgctgat gaacaacctc
    17581 gaccccgagg ttgccgagcg gcccgacgac ctcgtcgtgt acggcggcac ggggcgcgcg
    17641 gcgcgggact gggcgtcgtt cgacgccatc tgccggaccc tcacgacgct ggccgacgac
    17701 gagacgctgc tcgtgcagtc ggggagaccc gtgggagtct tccgcacgca cgccgacgcc
    17761 ccccgcgtcc tcatcgccaa cgcgaacctc gtgggacgat gggcgacgtg ggatgtgttc
    17821 cgcgatctcg aacggcgcgg tctgacgatg tacggccaga tgacggccgg gtcgtggatc
    17881 tacatcggga cccagggaat cctgcagggc acgtacgaga cgttcggcgc ggtcgcccgc
    17941 cgcgatttcg acgggtcgct ggcgggtcgc ctggtcgtca ccggcggcct gggtggcatg
    18001 ggtggggccc agccccttgc cgccaccatg aacggcggcg tgtgcctggc catcgaagtc
    18061 gatccggcgc gggtcaagcg gcgtctcgag actcgctacc tggaccgggc gaccgattcg
    18121 ctggatgaag cactgaagtg gtgtgaagag gcgcgcgcat cgcggaaagc cctgtccgtc
    18181 gggctgaccg ccaactgtgc ggacgcactt ccggaagtcg cccggcgccg ggtgaaggtc
    18241 gacgtgctga ccgatcagac gtccgcccat gacacgctga acggctacgt gcccgccgga
    18301 atgtccctcg tggacgcgac cgccttgcgg gccagggatc cggccggata cgtgcgcgcc
    18361 gcgaccgcgt cgatcgtcag gcacgtggag gcgatgcttg cgctcaaggc acaaggggcg
    18421 gtcaccttcg actacggcaa caacatccgg accgtggcga aggacgcggg gctcgacaac
    18481 gcgttcgaca tccccggctt cgtgcccgag tacatccggc ccctcttctg tgaaggcaaa
    18541 ggtccgttcc ggtgggtcgc gctctccggc gatccgaagg acctggcgcg gacggacgag
    18601 ctggtgctcg agctgttccc aaaggaagca cacctgcgtc gctggatcac gctcgcccgc
    18661 gaacgcgtgg ccttccaggg cctgccggca cggatctgct ggttggggca gggggagcgc
    18721 gcgcggttcg gtgtggcgct caacgatctg gtcgcgaagg gagagctgtc ggcgcccatc
    18781 gtgatcggcc gcgaccatct ggacaccggc tcggtcgcgt cccccttccg tgaaacggaa
    18841 gccatgcgcg acgggtcgga cgccatcgcg gactgggcca tcctcaacgc actgctcaac
    18901 accgcctccg gcgcctcctg ggtctccttc catcatggtg ggggagtggg gatcgggaac
    18961 tcgctgcacg ccgggcaggt gatcgtggcc gacggcacgc cggaagcggc ccgccggctc
    19021 gagcgggtcc tcaccaatga tccggggatc ggcgtcgcac gccatgccga tgccggttac
    19081 cccgaggcga tcgcgacggc cgagcgggag cacgtgcggt tgcccatgcg cgaggcgcgc
    19141 gactgagcac cctcctcgtc ggtgcccgag cggtggttac ctgccggggg ccggcgcggg
    19201 ctcggcgccg ggacgaggcc cggaccgtcg aggtcctccc cggaggtgcc gtcctgatcg
    19261 aaggcggcac gatcaccgcg gtcggtcctt acgccgagct ctcggcggcc catcccaaag
    19321 cgacccgtga agaggtccgt ggcgtgctgt accccgggtt catcgacgcg cacacccatg
    19381 gagtgttcgg ggcgccacgg ctcgacgatc accagcgccg ggccctcgga gcgacgtacc
    19441 aggcgatcgc cgcggcgggg ggagggatcc tccaatcggt ccgggatgtc cgggggcgca
    19501 ccgaagacga tctctatcgc ctggcgcgcg ctcggctcga tgcgctgcgc gcccatggcg
    19561 ccacgaccgt cgaaatcaag tccggatatg ggctcgagct cgacgcggaa atgaagcagc
    19621 tgcgagtcat cggccgtctg aaacgcgacg gcagccccga cgtggtcgcc accttcctgg
    19681 gtgcccacga ggtgccgcct gaacaccgcg cccatcccga tacctacgtc gccacgttgt
    19741 gcgaggacat gatccccgcg gtggtgcggg agcacctggc cgcgcggtgc gacgtgttct
    19801 gcgagccggg cgtgttcacc gtcgccgcga gtcgaaggat tctggaggcg gcgcggagcg
    19861 cggggctcgc gttgaccatg catgcggacg agctggaggg cgcgggcggg gccgagctgg
    19921 ccgcgacgct cggcgcgcaa tccgcggacc acctcgccgc catttcaccc gcgggcatcc
    19981 aggcgctcgc ccgaagcgaa acggtcgcgg tcctgcttcc cgccaccatg acgtttcttg
    20041 ggaagaagtc ccaggcgccc gctcgggccc tttgggaggc gggtgcggcc gtcgccctgg
    20101 ctacggactt caatcccgga tcatcgccca cggtcagtct cccgtacgtc atggcgttgg
    20161 gtgtctccca actggggatg cgccacgagg aggcgttgac ggccgtgaca gtcaatgcgg
    20221 ctgccgcgct cggcgtggcc ggggatcggg gccagatcgt accgggaatg cgcgcggatc
    20281 tggtcgctct cgacgtctcc gattggcggg aagcggccta ttggatcggc gcgaatctcg
    20341 taaccgccgt ctggacaggg ggttccgctt gtcgctccat cggcgggccg atatctttag
    20401 gcggctatgt cgaaacttga gaaagccaag gaacgtgccc gcgccctcga ggtgaaggat
    20461 gccaaggggg cagtcgacgc gtggatcgag gttctcaagg agcaggattc ctcaggtgag
    20521 ccgaaccctg atctggcgat ctacaacaag gtcggggatc tgtggctcaa gctgaaagac
    20581 cccggccagg cggcggacta ctacgaccgc gcggtcgaca aatacgccga gcaggggctg
    20641 cacaacaacg cgatcgcgat gtgcaacaag gtgctccgca acgcccccgg gcggcagaac
    20701 acctatttga agcttgccaa gctctacgcc gccaaaggct tcacgtccga ggccaagcag
    20761 aacttcgtcg agtacgcgga gcgcgtgcac aagacgggca acatccagct cgcgttcgcg
    20821 gcgctcaagg agttcacgga catttcgtcg gaaggggagc agctccgcca catgctggag
    20881 gagcagctcg ccgcgtacgg ggtgaatgag aacccccaac ggcgcaccac gcaggcgaag
    20941 gtcgtagagc cgccgaagac gccggggatg aagcgaaaga cgtcgagcct ggtgttcctc
    21001 gacctcgacg cgcccacggc tcccaaaggc ggggcagcgg caccagccgc acccaagccg
    21061 gcggccccgg ccccgaagcc cgctcccgct ccggcggcga aagctccggc ggtgcccgca
    21121 cctccgccgg caaaggcgcc cgcgcagact gccgcgccca aggtcgcgcc gccgccgagg
    21181 ccgaccccca aaccggcacc gccgccgcct cccgagcctg aaccggaacc ggtcagtctc
    21241 ctcgatgagc cgccggtcgc ggagcccgac acgtcgctcc agatcgagtc gacgagcctc
    21301 gcggacgagg ggatctccgc cggctcgggg tcactgctcg aggggctgga gtcgacgagt
    21361 gcggacttcg gcagcgtccg cgacagcgcg acgaatatcg agtcggtgcg cgaggagatc
    21421 gtcgcggacg acgtcgagcc gatggcggag ctcgagccga cggtggaaga ggaggtcccg
    21481 cctccgccgg cgcgtggcac gaggggccgc caggcggtcg tcgagctctc gctcgacgag
    21541 gagccgttgg tcgaagccga gccggtcatc gaagcggagc ccgaggtcct ggccccggtg
    21601 gacgacgagc ctctcctgct cgacgacggt gccggccagg agacgcggga cttcatcatg
    21661 accgaggatg acacctcggt gggcggacgt ccctccgtgc acatcgacgt cggcctggat
    21721 ggcggcggca tgcagatcga agattcggcg ccgtccatcg acttctccga catcgaagaa
    21781 gcgccgacgg tttccatcga agacctcgag ggacgtgtcg cggacaaccc ggacgacccc
    21841 gaggcgcacc aggcgttggg cgaagccctg atcgaatcgg gtgaccggga acggggcatc
    21901 gaggagctcg accacgccac cgcgggcttc gagagcgcgg ggaattccca gcaggccgag
    21961 gatctggtcg acgagattct gcgcctcgat ccgaacagca tccgccaccg gcagaaggtg
    22021 gtggagttcg cgttcaagtc gggggagaag gatcggctga tcactgcata cctcgagctg
    22081 ggcgatacgc tcctgcgcgg ggacctgccc gacaaggcgc gcgccgtgta cgagcgcgtg
    22141 gccgagcatg atccgaagaa cgcccgggcg cagtcggccc tgtccatgct cgcgcccgcg
    22201 gctcccgccg cgcccgaggg aaaggtcaag gcgaaggacg cgaagatgaa agtccgggac
    22261 gaagcggcgg agggcgccga cttcgtggat ctgggttcca tgatcctcga cgatcacggc
    22321 gaggagcggg actcccggat gaaggtccag gacgaggagc cgaccggcga cgagcagcag
    22381 gatttccagg agatgctcgc gcggttcaag caggggatcg acgaaaacat cgaggacacc
    22441 gacttccagt cgcactacga cctcggcgtc gccttcaagg agatggggct tctcgacgaa
    22501 gccatcgccg agttccagaa ggcccttcgc gcgcaggacg ggaagctgcg cacgtccgag
    22561 gcgttggggg tatgcttctt cgagaagggc gcgttcgggg tggccgagtc gatcctgcgg
    22621 cgcgggctcg agctgccgtc ctcgggagag gaggagcggc tgggcatcct ctattggctg
    22681 ggccgtgcct gcgaagagca gggacgtccc gccgaggcga aggacctcta cgggaaggtg
    22741 ttcgcggtga acatccggtt cctggacgtg ggcgagcgcg taaaggcgct ggccaaggcg
    22801 cgctgagatg agtcagcaag tcgcccccgt caccctgcac gacatccagc gtcccgtggc
    22861 gcaggacttg gtgcgcgtgg ccgaggagct gcggcgcatc atcgccgcgg acttcccgag
    22921 catcgccgaa gtgaacgacc atctcctgcg gatgaagggg aagctgttcc ggcccatgtt
    22981 gctcctgctg tcggcgcagg cgaccgggaa caccgagccc cggagcgtct cgctggcggc
    23041 ggtggtcgag ctcatccatc tcgccacgct ggtccacgac gactcggtcg atcattccgc
    23101 gctgcgccgg ggactcccga cgctcaacgc gttgttctcg catcagatcg ccgtgatcat
    23161 gggcgactat ctgtactcgc gcgccatcat cgagctggtc aaggccgaag acatcgagcc
    23221 gctgcgcgtc ttcgcccggg tcacgaacga aatgaccatc ggcgagatgc ggcagatcga
    23281 ggcgcacgac aagctgagct acaacgaagc gcagtacgac cacctcatcc gcgccaagac
    23341 cgcatcgctc ctgtcgggcg cgtgcgaatg cggcgccctg cggggcgacc ccgcgcaccg
    23401 cgccgcgctc gccgcgttcg ggcttcacct cggcatggcc ttccagatca cggacgacct
    23461 gctcgactac acggaggcgg aagccgtcac cggcaagccg tggggtctgg atctgaagga
    23521 gcacaaggtg acgctcccgc tcatcgcggc gctgccacgc atggcgcccg aggggcgccg
    23581 cctggtggag cgcttgatgg aggagccgga accgtccgat cacctcgtga cggacgtcat
    23641 cgcctgggtg aacgaggccg gtgggctgac gtacgcacgg cagcgcgcgc tcacgctggc
    23701 acaggaagcg gaacgcgccc tcgacggcct cccggcgtcc ccggctcgcg acgcgttgcg
    23761 cgacagtatc gcgtacgccg tggagcggag gagctagcgc gtggcgcggg gaccgcggcg
    23821 tccgctattt tacctcggag tcctgacgac cgggttcgtg ctgggcggct tcatgcaggc
    23881 gctgctccgg caatttctgc cggcgggcgc cgcgaaagaa tttttcacca ccgcggtgca
    23941 cccgaccttg gggccggtgc agattaatct gctggtggtg gacatcacgc tcggtccgat
    24001 tggactggac gtgtcgctgg tggcggtact cggagtggtg atcgcctact tcgtggcacg
    24061 gtcgcttttc taagagagag gctggtatgg aactgtcggt ctggcacatc gccctcttgc
    24121 tgctggtctt cctcctggtg ttcggggcga agcgcatccc ggagatcggg tcgtcgctgg
    24181 ggcaggggat ccgcgaattc aagcggagca tcaaggagat cggagacaac ccgccggatc
    24241 agcggaccaa cgttccgcct ccttcggcca ccaactcgac gctgccgccc acgggcggag
    24301 agccgaagcg tctgagccag tagggccgcg gatgtcggtc gcagggttct cgatcggcga
    24361 gtggctcgtg ctgctcgccg ttctttttgt ggccgtggga ccgcgccgct tgcccgaagt
    24421 cgggcgggcg gcggggaagt tcctgcgcga gttccggaag ggcctgaacg aagcgcggga
    24481 cgcgatgggg cggcccgacg tgccgcttcc tccgcgtccc gcgaaaccga cgagcctgct
    24541 cgactagttc aactgcccgc ctgttgggtt tgctggaaca tttccttccg ccggtccacc
    24601 accttggcct tggccttgag cgccgcgaga tacgccgtca ctcgcgcttg ctggagcccg
    24661 cgcaccagca cggtgcgctg ctgatcccgc tgggccagcc acgccgtgga atccgccttg
    24721 ctccgggaga tgccctggag caggaagtca ccggtccgcg cctcgatcaa gggactgcgc
    24781 tcacccggct tcagcccgaa cgccgcgccg accacttccg ggtccagctg aatctgggcg
    24841 ggtgggcgga accgcgtgaa cggtccgatg gtggtggccg cgtaccccgc cgccgcggcc
    24901 gcctgcctga ggtcggaggc accggcgagc ttcgccgcca acgcctgtcc ccgctgccgc
    24961 acgatttcct tcttgcggtc gaacgtggcc gcggagtcga tccgcgcccg aatctcggcg
    25021 agcggcggaa cacccgcggg acggagggaa tcgagccgga acacgtagaa cgcccgctcg
    25081 ccctcgatca ccttgctggt ctgtccgggc cgggcgccac cgaacgccca agtgccgacg
    25141 tcagggatga catacttgcc cagcgtcatc cgctcgccct gatagatcgg gggagccgtc
    25201 gcgacgttca gatgcatcgc cttgcccagt gagtcgatga cgctgccgtc atcatgctcg
    25261 cccgccaggc ggtcgagcgt gtcggcacgc gcttcgacca ggtcgaggcg gggtcccgtc
    25321 agcgcgatgg ggaagaggat gtgccggacc tccaccgagt cacccgcggc cttgtcgacg
    25381 cggatcaggt gatagccgaa ggccgacaag acgggctggg agacctgtcc gggctgcagg
    25441 ttcttgaggc cggcgatgaa gccggcgtcg aagccgggct catcccgctt gatccacccc
    25501 aggtcgccac ccttcgctcc cgtgatggtg tccaccgatt gccgcttggc gacccgcgcg
    25561 aacgcggatt cgctgaccat cacttccggc cggaccttcc gtgccagcgc gagcgccgcg
    25621 gcggtgtcgg acgcctcggg agcgcggggc aggacgagga acgtggtgta cgcgatcgcg
    25681 ggccgcttga attcgctcga gtggaccgtg tagtagtcct ggatatcctt gtcggtcagc
    25741 ttcacggcgg agtccgggat cgacacgttc tggatcgcca ccatggcgac ggtcgcggag
    25801 tcgtgctggt cactccagat gcgccagagc ttggcatcgg ggacatacac gtcggcggtc
    25861 aggtactccg cgagcttctg ctgcgggatg accgaccggt accgcgcttc cagctggacc
    25921 atcaggtccg gactcgctcc cgatgccaga aaccgcagcc acttgttcat gtcgaactgg
    25981 ccgttggtct gaaaatcggg gctctgcatc acttcgggag gcggggagct cttcgcggcg
    26041 tcgatcacgt cctggtcgga cacggtgatg cccagcttcg cgtacgactg ctgcagcatc
    26101 acgtcctggg tcagctggtc caccacctga tcttcgagct gcttctgctg gtcatcggtc
    26161 agggaggcgt tgcccccccg cgcgacgtac tgctcccggg cggcttccac cgcggcctgc
    26221 cactgcgggt agtggatttc ggtgccgttg atcttcagaa cgacgtcgcc cccggtcttg
    26281 acccccgtga cgttcatgcc gacgtcgaaa atcatccatc cgataaaggc aattgcgagg
    26341 acgtagaaga tgtacttcga gttctcgcgc atggtgcgca tcatggcggg gcctggctcc
    26401 ggtcaaaaag gggggtgaag gggcataagt ctaaaagcca caacgaggaa actcaaccga
    26461 aacctcgcgc gaaacgttga cagccaagtc ggccaaacac tatcttggac caattcccac
    26521 aaagacttag gcccatttgg cgtacaccag cgagatcgag aagctcgagc gacgttggct
    26581 cgagaatccc aaggggcgca attttgcgcc cttggcggat gcctatcgca aagcaggaga
    26641 aatagatagg gccatcgacc tctgcaaaag cggcctcgag ctccacccgg actacgtcag
    26701 cgcccacatc gtgtacggcc ggtgtctgat cgaccagaag gacgaccccg ccgcgaccga
    26761 agtgttccag aaggtgctcc aactcgaccc cgaaaacatc ctcgccctca ggatcctggc
    26821 ggaaatcgcc gaacgaaccg gccattacga ccagggtgcc gagtggctgg gacgcctcat
    26881 ttcggccgac cccatgaacg gggatgccgc cgaagccctg acgcgcatgc ggtccaaggc
    26941 ggcccaagcc aagacggcac ctcgcgcatc cctgccggcg gtcgaagcgc cggccgaaac
    27001 ggtggcggag atcgttcccg agaagtccga gccctcggca gacctcccga tcgagcgggc
    27061 cagctcgccg aatgtcccgg cggagcttcc tccgcgcctg tccctggtgg acatggcgac
    27121 cgtgccgatg aaccagcccg atttcggcgt ggaacgggcc agcgggtcag gcaaggtcat
    27181 cgatgcggac cggctggtcg acgcggagcc cgagctcccg aatgccgccg gggcggaggc
    27241 cgcgaaggac cagcggctct cgctgggcct cgaggccgaa ccggagctcc cggggcaagg
    27301 ggcggtcgct ccccgtaaca acatggggtt ggagagtttc gacggcatcg atttctccaa
    27361 cgcgccgcct tcgcagcgga tggaaggcat gatgtcggag gaggggccgg ccccgaatgt
    27421 cgacaacgtc gaggtcgagg gactggcgcg cacgcagtac gaggggagcg gcacgttccg
    27481 gctcgatcag ccgctcctcg agagcaccgc gacaccgcac ctcgaacctg acggaccgcc
    27541 ggtgtccctg ccgctcatca tgcccgacga tgtggagccc ggtacggccg aggctcctca
    27601 actgacgcca ccgccatctc ctccgcccgc gccgcctcgt gcctcaaggg tatcggcgcc
    27661 ggcgccggtc ccggcaccgc cgctccctcc cgatgatgcc gaggacgtag gggccgccga
    27721 cacggcgacg ctgtcgcagg cggaaccggt gctcacggag acgatggccg agctgtacct
    27781 caagcagggg cacaaggacg acgccctgcg ggtgtaccag gcattgctgg cgcagcgccc
    27841 cggcgacgcc cggctcgagg cgaaggttcg cgagctcgac ggctcggatg acgcgccggt
    27901 gctcacgtcc cacccgatgc gggcccccgc cagcgggctc tcgctggggt ccttcctgaa
    27961 aaggatcctt ggctcccggc ccggcgcacc cgcggccgat ccgcttcccg aggagtcggc
    28021 gctcccgggc ccgcgagtga cgtcgggcgc gacgccgcag tccatgctgg cggacgcatt
    28081 cgccgatgag ccgcccctgg gccccacgcc cggctcaccg agccggcccg cggcggacag
    28141 cctgtcgctg gacgcggtgt tcggcgatga cgggagccgc gcgtcggccc cgagcgtcga
    28201 ggcgccgcat gaagccgcgg cttccgcgcc cgctccggcc gcgccgtcga agggcgggtt
    28261 ctcgttcgac gacttcttcg ggtcgggcgg cgcaccggct ccggccgccg actccccggc
    28321 gcctgcggcc gcgtcccacc ggccttcagg atccgctccc cggccctcga cccggacgtc
    28381 acgtccgcag gcgccgttgg aggacgcggg cgacctcgac cagttccagg cgtggctcaa
    28441 gggtctcaag tcctagtgcg cgtcgcggta ctgaatgggc cgaatctcaa tctgctgggg
    28501 gagcgcgagc cggagatcta cggagcggtg acgctcggtc agatcgagaa ggccacgcaa
    28561 gaacgcgccc gcgcccttgg cgtcgagtgt gtgttcagtc agaccaacca tgaaggcgag
    28621 ctggtcgacg cgatccagaa gctcaaaggg tcggccgacg gcgcgctcgt gaatgccgcg
    28681 gcgttcacgc acacgagtct cgccgtccgg gatgcgctcc tggcggtccg ggtgccgttc
    28741 gtcgaagtgc atttgtccaa catcttcgcc cgcgagcccg agcgtcgccg ctccctgctc
    28801 gccgacgcgg cggtaggcgt ggtcgcgggt ttcggcgcgg acagttaccg gctcggcctc
    28861 gaggggctcg tggcgcatct cgcggcccgt cgtgcccgct gatcgccggc ccacgcgccg
    28921 gcaggcgctg accgaatccc tcgccgcgca gggtctcgac gcgctgctga tcacctcgcg
    28981 cgcgaatgtg cgctatctga cgggattcag cgggtcggcg gggatggtgg tcctcacgcc
    29041 gtcggagctc ctgctcgtca cggacttccg gtacgacgag caggcccggg acgaagcggg
    29101 cagtgtggcc cgcgtcgagg tcgagcgaac cagcgtctgg gaccggctct tccgggagat
    29161 gggacggctg gggccaatcg agtcggtggg attcgaggga cacgccctca cggtgcgcga
    29221 ggccgagcgc ctggtggcgg ggggcaagcc ctggcggtgg caacccgcgg gcgagctggt
    29281 cgaggcgctc cgggtgcgga aagatcccgg ggagatcgcg gccattcggg acgcggcgac
    29341 gctggcgacc gacgcacttc gggaaacgct ggcgcgggtg cggcccggct tgaccgagaa
    29401 cgaaatcgcg ggcgtgctcg aaggagcact tcgcaagcgg gggagtgagg cacacccgtt
    29461 cccgaccatc gtcgcttcgg gtccgcgcag tgccctcccg catgcgcgct cgagtgaccg
    29521 gcaggtcgcg gccggcgaat ggctgttgat cgacttcggc gcgaccgtcg atgggtacgc
    29581 gtccgacatc acccggaccg tggtcgtagg cgcgcgggcg acggagccgc agcgggcctt
    29641 gtacgacctg gtcgcagggg cccagcggcg cgcccgtgag ggcgtccggg cgggactgtc
    29701 gggtcgtgat gcggacgcgc tggcccggga ccccatcacg gcccggggtt ttggcgaggc
    29761 gttcgggcat tcactggggc acggcctggg attggacgtg cacgaagcgc cccggctgtc
    29821 ggggacggcc accgaagcgt tgcccgtgga tgcggtcgtg acgatcgagc cgggcgtcta
    29881 cctcgccggc gaaggcggtg tccgcatcga agacgacgtg tggctctcgg ccgaagggcc
    29941 ggtgctcctg tccgatgggg ccacggaact gctggaactc acgcaggatt gacggatcaa
    30001 ccgggcgcaa gctctatttg gtgaaatgaa cctcgatctc atcagccaac tggtcaaaac
    30061 tctcaaggat gccccggagc tggggtccat cgagattcgc cgcgggtttc tcggtctctg
    30121 ggcgtcggtc cgggtctccc gggccgggca gggggccgga ggtgccgcac acgtcgtggt
    30181 ggggaacgcc ccggcgacgg ccatgccggc ggcgccggcc acgaccgcgg ctccggccgc
    30241 tgccccggcc cccgcgccgg cacccgtatc caatctggtc gaggtcaagt cccccatggt
    30301 ggggactttc tacaagtcgc ccgaacccgg cgccgaggcc tacgtgaagg tcgggtcgcg
    30361 ggtcaccacc ggacaggtgg tctgcatcat cgaagcgatg aagatcatga atgagatcga
    30421 gtccgaagtg cagggcgtgg tccgcgaagt ctgcgtggag aacgcgcagc ccgtcgaatt
    30481 cggtcaggtc ctcttccggt tggatcccaa tggctgagac acccgccgcg ggtacggccc
    30541 cggccttcaa tttcaagcag gccatcaccc agatggtgca gcgcaacggc tcggacctcc
    30601 tgctcaaggt gggacggccg gccacggtcc gcgtgaacgg ggagctggtg gcgctcgaga
    30661 ctcagcccct gaagccggag gagctgaagt ccctcgccga acagatcatg acgccgcggc
    30721 aggtgaagga gttcgcggag cacaaggaag cggacttcgc catcggcgtg ccgggggtgg
    30781 gccgcttccg gaccaacgtc taccagcagc gcggcaccgt cgccttcgcg ttccgggcca
    30841 ttccgtacga agtgaagacg gtaaaggatc tgtcgttgcc gggggtgctc gaggaaatcg
    30901 cgctgaagcc gcgcgggctg gtgctggtga cgggggtgac ggggtcgggg aagtcgacgg
    30961 ccctcgccgc gctcatcaac cacatcaacc tgaaccgccg ggtcaacatc atcaccatcg
    31021 aagacccgat cgagtttctg caccgcgaca acctcgcgaa catctcccag cgggaggtgg
    31081 gcaacgacac gctgtcgttc ggatcggcgc tccgccacgt gctgcgccag gatcccgacg
    31141 tcattctgat cggcgaaatc cgcgacatgg agacgctcga tacggcgctc aaggccgcgg
    31201 acacgggcca cctcgtcttc tcgacgctgc acaccaccga cgcgacgcag tccatcaacc
    31261 gggtcatctc gttcttccca ccccaccagc acgacgaagt gcggcacctc ctgtcgacgg
    31321 ccttgcaggc ggtggtttcg ctccgcctgg tgccgcggaa agacggcaag ggacgagtcc
    31381 cggccgcgga ggtcttgatc aataccgcga gcgtgcagga caacatccgt gacctcacca
    31441 aggcgctcgc cattcccgat ctgatcgccg agggctcggt gcagtacggg atgcagtcct
    31501 tcgaccagtc gctgttcggc tggtaccagc agggcgtggt gagctacgag agcgcgctct
    31561 tctatgcctc gaaccccagc gagttcgcgc tgaaggtgtc gggggtggat tcgcaggcga
    31621 gccgcgcctt cggcggatcc gccggtggcg gcgccggcgc gatcgacttg actccctgat
    31681 gttcaagaaa gtcctcatcg ccaaccgcgg ggaaatcgcc ctgcgggtga tccgcgcgtg
    31741 caaagagctc ggcatcgaga ccgtcgccgt gtattcggag gcggaccgcg agtgcctgca
    31801 cgtccgcttc gcggacgacg acgtctgcat cggacgggcg ccgagccggg agagctacct
    31861 caacatcccg cgcatcatcg ccgccgccga gatcaccggc gccgacgcga tccatccggg
    31921 atacggcttc ctggccgaaa acgcggagtt cgcggacatc gtgcgggcct cgaacatcac
    31981 gttcatcggc cccacgcccg accagatccg gcagatgggc gacaaggcgg tggcccggag
    32041 catcgcgcag aaggtcaaga tcccgacggt gccgggctcg cccggtccgg tgagttcggt
    32101 ggaagaaggg ctcgaattcg ggaacaagat cggctttccg gtcatcatca aggcggcggc
    32161 gggcgggggc gggaagggca tgcgggtcgc gaccgatccc gactcgttca cgaagagcta
    32221 caacctggcg cggcaagagg ccctcgccgc gttcggctcc gatcaggtct acatcgagaa
    32281 gtatctcgcg cggccccgcc acgtggaaat ccaggtgatg ggggataccc acggccgggt
    32341 gatgcacctc tgcgagcgcg actgctccat tcagcgccgg catcagaagc tggtcgagga
    32401 agcgccgtct cccgccctga accaggagct tcgcctcaag atgggacagg cggccgtcac
    32461 cgccgccgag gcgatcaact acgtcggcgc gggcaccgtc gaattcctcc tcgacgaaga
    32521 cggctcgttc tatttcatgg agatgaacac ccggatccag gtcgagcatc cggtgacgga
    32581 gatgtgcacc aacttcgacc tggtgaagga acagatccgc gtcgcggcgg gggagtccct
    32641 caagctcaag ctcgaaggca cccgtctgcg cgggcacgcc atcgagtgcc gggtgaacgc
    32701 cgaggatccg ttccgcaact tccagccctg cccggggcac atcagcgcgt atcatccccc
    32761 cggcggtccc ggggtccggg tggacaccca catctacgac ggctatacgg tgccgccgta
    32821 ctacgattcc ctcctggcca aggtcatcgt gcacggcaat acccgcgagg aagccctcgc
    32881 ccgtatgcgc caggccctcg actcgtttat cattgagggc gtgaccacga ccattccctt
    32941 cctggggcgc atcatgcgcc atcccgactt catcgccggg aaggtcgaca ccaaattcct
    33001 cgagcggcaa ccgcaactct tcaaagaatg aagatcgacg tgcacttcac cccgctcggc
    33061 ctctccggag aagtcccggg ccgggcggtg gtcgttctcg acgtcctccg gtccaccacc
    33121 accatcgtga tggccctcgc caacggcgcc aaggccgtgg tgcccgccgc ctcgacggaa
    33181 gaggcggtcc gcatgacgtc gaatctccag cgcgatgcct acctgctcgc cggggagcgg
    33241 aagtccctca agatccccgg ctttgcgctc ggcaattctc cgcgggagat gagctccgac
    33301 gcggtcgccg ggaagacgat ctttctcgcg acgacgaacg gcacccccgc gctcgtcgcc
    33361 tgcgcggacg gcggcaccgt gctggtcgga ggcgccatca acttcacggc actggccgac
    33421 cgcgcgcggg agctgttcac catccagagt gagctggtca tcgtgtgcgc cgggcgggaa
    33481 gggaagttcg ccctcgagga cgcctacacc gccggccgga tcgtgaaggc cgtcaagaaa
    33541 ggcatccgca agctcgacct gaacgacgcc gccagggtgg cgctcgggct caccaccgag
    33601 tacaccacct ggaccgaggc cttcgaagcc tcggacgcgg cccgccagct gatcgaagcc
    33661 gacctcggcg acgacatcgc gttctgcgcg aaagccgatc gctgtgccgt cgttcccacc
    33721 tacgcggacc gccgcatcac gtgaacaaca gagcgcgcga agagttggtg ggcatcgggg
    33781 ccctggtcgt cggcctcttc ctcggactca cgctcgcgcc ctggcacgtc accggcaaca
    33841 tcggctccgc cgtgggcgac acgctctgga aggccttcgg cgtgggctcc atcgtcattc
    33901 cggtgctcgg catcggctgg gcgctcgccg cgttcggccg cctgggctcg gtctccagcc
    33961 cgcgcgccgc catcctcggc gccggactga tcgtgctgct gccgtacgcc gtggccgtcg
    34021 tgaaggggtt ctccgccccc tattcccccg actacgcggt ctggagcgcc tcacagcgcc
    34081 tcgtcggcct cgtgcctgcc tttttcgccg aggtggtcga acgcaccacc ggtcccgccg
    34141 gtgcgaccct catcggcctc ttcgccctct cggcgctcgg ggtgttcacg attgggtggc
    34201 atccgctggc ggtgctgcgg gccgggcccg acgaagagac gaaggccggc aagcggcaag
    34261 cgaaggacga gaaacgggaa acgggaaagg tgaaacgtga ggccggtctc acgcctatgc
    34321 ccgccagcga tgtggacgag gaggcggagg cgccgattcc cgtttcccga ttcccgaagg
    34381 accgaaaggc caaaccggct ccgaaggtaa aaccggttgc caaggtgccc cccccgcctc
    34441 ctcccgaggg tctcatcccg cccgtcgagc tgttgaacga gccgtccgcc gaggcggccg
    34501 acgtcgggat cgcggaaatc gatcaaatgg ggcagcggtt gatccagacg ctcgagacgt
    34561 tcaaggtcgg cggccggatc gccgaccgga cgatcgggcc cgtggtgacc cgtttcgaga
    34621 tcgagccggg tcccggggtc aaagtgggac ggatttccgg gctggcggac gacctcgcgc
    34681 tgtccatgaa ggcgcagtcg ctccgcatcc tggcgccgat tccggggaaa gccgccgtcg
    34741 gcatcgaggt gccgaacccg catccgcgga tggtcgtcct gagggagctc ctgtccgggg
    34801 atttccgcag tgaccgcatc cttcccgtgg cgctgggccg gaacatcgag ggcgaagaag
    34861 tcgtcgcgga cctcgccaag atgccgcacc tcctgatcgc gggacaaacg ggctccggaa
    34921 aatcggtctg catcaatacg atcatcacca gcctgatcta cgcgtatccg ccggagcggc
    34981 tcaagctcct gatgatcgat cccaagatgg tcgagctgtc gatgtacggc tcattgccgc
    35041 acctcggcat gcaggtggtc acgaaccacc acaaggcggc gcgggtgttc aagtgggccg
    35101 tgcaggagat ggaataccgg tacaagctcc tgcacgcgaa tcacgcgcgg aacatcatcg
    35161 acttcaacaa gaaggtgatc gagaagaagc ccctcaaggg tccgcaggag accctggcgt
    35221 cgcaggaggg gattcagaag gagctgaagt tcgaggcgga gtacaaggat ggcatcgtcc
    35281 cgtacgtcgt cctggtggtg gacgagctgg cggatctgat gctgacggtg cagggggaga
    35341 tcgagacgcc cctggcgcaa ttggcccaga aggcgcgtgc catcggaatc catctcctgc
    35401 tggcgacgca gcgcccgtcg gtgaacgtca tcaccggcgt gatcaaggcg aatttcccga
    35461 gccgcatcgc gttccgggtg gcgtcgaagg tcgacagccg gaccatcctc gatcagaacg
    35521 gcgccgaatc cctgctgggc aacggcgaca tgctcttcct gccgccgggg aagagcgaac
    35581 ccgtccgtgt gcagggagcc ttcatcggga ccgatgagac cgagaggctg atggactggt
    35641 accggggacg cggcgccgag cggcccgagc attcggcgat cgaagcgcag gtcgaggcca
    35701 tcgaggcgca ggagtccgcg caagccgcgg ccgaaggctc cggggacccc ggcgagcgcg
    35761 atccgctctt ccggcaggcc gccgaggtgt gcatccagaa ccagctgggc tccacatcgc
    35821 tcctgcagcg ccgcatgagc atcgggtacg ggcgcgccgc ccgcatcatc gatcagttgg
    35881 agatggcggg agtgctgggg ccggcgaatg gctcgaagcc ccgggacgtt ctggtgggat
    35941 tggaagactt ggatgaaata gtgggggcgt aataagaaaa ggcgtcgagc aagagcgtcg
    36001 agcagaagcg tcaagcaaaa ccgtaccgtc gtgcgttaat tgggttatgt cccccaacgg
    36061 ggagagcgta gtttcacccc cgtgagctcc ggggtacgaa gtgatccaga gacttgggaa
    36121 gatccgcggc accgggaagg cctggccggc gagctgcagg cgatcgacta tctgaaaacc
    36181 cagggctggg tgctgatcgc gcaccggtgg cgcgtcgggc gcatcgagat cgacctggtg
    36241 gtgcgtcgcg ggaagctggt ggcgttcgtc gaggtcaaaa ccaggcgagg ggacgccttc
    36301 gggcacccct tcgaggcggt caccggtgcc aagcgccggg agatcgtcaa agcggcccgg
    36361 gcttggaccg atcgccatgg cgcaccggac gatatctacc gattcgactg tatcggcata
    36421 cagaacaaca agttaacgca cctcgaggac gcatttcggc ccggttggcg ctaaacaacg
    36481 cttcggctta tattcgggct caccactcaa gctcgaggcg ctctaccaat gatgacgagg
    36541 actcctgtgg cggcagaccc ccaagaaaac ccgaagctgt cggatgaccg ccggaaggcg
    36601 ctcaacctcg ccatcaccca gatcgagaag agcatcggca agggcgccat catgcggatg
    36661 ggtgccgaag cgcgtgttcg cgtggaggcc atcccgaccg gcgccatcaa cctcgatgcg
    36721 gccatcggca tcggcgggat cccgcgcggc cgggtgaccg agatcttcgg tcccgaatcg
    36781 agcggcaaga ccacgctcgc cctgcacgtg gtggcccggg cgcaaaaagg gggtggcgcg
    36841 gccgcctaca tcgacgcgga gcatgccctc gacgtcgagt acgcgaagaa gctgggtgtc
    36901 gacatcgaca acctcctcgt ctcccagccg gacaccggtg aacaggccct cgagatctgc
    36961 gagatcctgg tccggtcggg ggcggtggac gtcgtggtgg tggactcggt cgccgcattg
    37021 gtaccgaagg ccgaaatcga aggtgagatg ggtgactccc acatgggcct gcaggcccgg
    37081 ctgatgagcc aggccctccg caagctggcg ggtgccatca atcggtcccg caccgccgtg
    37141 atcttcatca atcagcttcg tgagaagatc ggcgtgatgt tcggcaaccc ggaaaccacc
    37201 accggcggcc gcgcgctcaa gttctacgcc tcgctccgcc tcgacgtccg gcgcatcggc
    37261 ccggtgaagg agcgcgagac ggtgatcggc aatcacgtcc gggtgaaggt cgtgaaaaac
    37321 aaggtggcgc cgccgtttcg tcaggcggaa ttcgacctga tgttcgacga gggcatcagc
    37381 cacgaatcgc tgctggtcga catcgccgcc gaagccggca tcatcgagaa ggccggcgcg
    37441 tggtacgcgt acggcaagga gcggatcggg cagggccgcg aaaatgccaa gatgtacctg
    37501 aaggatcacc ccgagatggc gctcgagatc gagctcaagg tcaaagaggt gctcgggatg
    37561 aagagctcgg ccgccggatc taccgagacg gcgaccgatg aggaggcccc cgcggcggag
    37621 gagtagtgcc catctgcacc ggactcgccc ccgatcctcg ccgccccggc taccgcgtgg
    37681 tggaagtgga tcgggggcgt ttcgcgtccc tccc
//
LOCUS       DASUBP010000117         3146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_193530, whole genome shotgun sequence.
ACCESSION   DASUBP010000117 DASUBP010000000
VERSION     DASUBP010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_193530"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VFU75_04400"
     CDS             <1..408
                     /locus_tag="VFU75_04400"
                     /inference="COORDINATES: protein motif:HMM:NF019238.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1569 domain-containing protein"
                     /protein_id="HEU5466105.1"
                     /translation="WNSAQIVEHLSISLEGSGRAFDTHRDKPPMARRPKTRVEWVASF
                     LILKVGWFPPGREAPQGSRPAEHVDRKYAEQHFREGIERFNRIAPDLLTSRPNDLFVK
                     HPRLGDLTLPEWMRFHLVHCRHHAKQIAARCAG"
     gene            complement(562..1278)
                     /locus_tag="VFU75_04405"
     CDS             complement(562..1278)
                     /locus_tag="VFU75_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466106.1"
                     /translation="MDAKGIGAVAALGEPARLALYNYVVARGEAGRDEAAKGTGISRA
                     LAAFHLDRLVAEGLLTATYRRRGGKTGPGAGRPAKLYTRAPGEISLSLPERRYELAAR
                     LLARAVAKAPGSAAARAVEGAASAYGRALGHEARIRAGARPSSAKLVAATLGLLAEQG
                     FEPVRDASGTIRLRNCPFHALAMECRDLVCGMNLSLLRGVIRGLAGAGLTAVLDPQPD
                     RCCVILKTRRASAPRADARG"
     gene            1383..1670
                     /locus_tag="VFU75_04410"
     CDS             1383..1670
                     /locus_tag="VFU75_04410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466107.1"
                     /translation="MPFGAFINAIPMPVFLVIHLAAFAIGAFFAKKAFGGGASEFGWG
                     FTLFALAEISYLTYHLDWTVFLFAHTISEVLDLLAFIMIFAGAAKSVLARK"
     gene            1667..2065
                     /locus_tag="VFU75_04415"
     CDS             1667..2065
                     /locus_tag="VFU75_04415"
                     /inference="COORDINATES: protein motif:HMM:NF012355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="plastocyanin/azurin family copper-binding
                     protein"
                     /protein_id="HEU5466108.1"
                     /translation="MTRSVSRGLRMGAVALVVAISGACHRAPPAPPVATTRVELPPSY
                     LFAPADIVVHAGATVVWTNHDHFTHSIHLTDDGGTSMMVPPGDSVRFTFTRPGLHHYD
                     CSLHPHDMHGTVLVQGALRDSTSGGISGSP"
     gene            2062..>3146
                     /locus_tag="VFU75_04420"
     CDS             2062..>3146
                     /locus_tag="VFU75_04420"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5466109.1"
                     /translation="MKRFQHLIVGGGMTAAAAAHGAREQAPKASVGIISAESHPPYNR
                     PPLSKALWKGDPESSIWRTSDVEGVEVILSRRVTAIDPAKKTATDDHGGTYGYETLLL
                     ATGGAPRRLAGAPDTIVYFRTLDDYRALHAAAAPGKHFVVLGGGFIGSEVAAALRMRG
                     AAVTMVIPEAGVGARVFPAPLSQNLVEYYRSKGVTVRTGEGVATVSENAGKAVVKTTT
                     GLEIPADGVVAGLGIIPSIELAQAAGLKTDNGIVVDEYLRTSNPSIYAAGDVANFPSK
                     ALGHRSRVEHEDNANAQGRQAGRNLAGANERYDHLPFFYGDLFELGYEAVGDLDARLE
                     TVSDWKAPFREGVVYFLGEGRVKGVLL"
BASE COUNT          511 a         1079 c         1045 g          511 t
ORIGIN      
        1 tggaactcgg cacagatcgt cgagcacttg tcgatttcgc tcgaagggag cggcagggcc
       61 ttcgacaccc atcgcgacaa gccaccgatg gcgcgtcgtc cgaagacgcg tgtcgagtgg
      121 gtcgcgagtt tcctgattct caaggtgggt tggtttcccc cgggacgcga agcgccacaa
      181 gggtcccgcc cggctgaaca cgtggaccgg aagtacgcgg agcagcactt ccgggagggc
      241 atcgagcgct tcaacaggat cgcgcccgac ctgctcacct ccagaccgaa tgacctgttc
      301 gtgaaacacc cccgcctcgg cgatctcacc ctgcccgagt ggatgcgctt tcatctggtc
      361 cactgccgcc accacgcgaa gcagatcgcg gcgcggtgcg ccggatgagc aggaagcgaa
      421 ggagcccgac gaagatccgg gtccccaatc cggacaagcc ggtgcgggag aggttgaaga
      481 ctcctgccca tcagcgccac aagtcgaaaa aagactatgc gcgccggccc aaacacccgg
      541 gtcaggtaga agagtagtgt actaaccccg ggcgtcagcc cggggggcgc tggcgcgccg
      601 agtcttgagg atgacgcagc agcgatccgg ttgcggatcg agcacggcag tcagtcccgc
      661 cccggccaga ccccggatca cgcctcgcag cagtgacagg ttcatcccgc acaccaggtc
      721 ccggcattcc atcgccaggg cgtggaaggg acagttgcgc agccggatgg tgccgctcgc
      781 gtcccgcacc ggctcgaacc cctgctcagc cagaagtccg agtgtggcgg cgacgagctt
      841 cgccgaggag ggccgcgctc ccgcgcggat acgcgcctcg tgccccagcg cccgtccgta
      901 cgcggacgcg gcgccctcga ccgctctggc cgcggcggag ccgggggcct tggcgacggc
      961 ccgcgccagg agacgcgcgg ccagctcgta ccggcgctcg ggaagcgaca gcgagatctc
     1021 gccgggggcc cgcgtgtaga gcttggcggg ccgcccggcc ccgggaccgg tcttgccgcc
     1081 acggcggcgg tacgtcgcgg tgaggagtcc ctccgccacc agccggtcga gatggaaggc
     1141 cgccagggcc cgcgaaattc cggtgccttt ggccgcttcg tcccgcccgg cctcgccgcg
     1201 cgcgacgacg tagttgtaga gggccagccg ggccggctcc cccagggcgg cgaccgcccc
     1261 gatccctttc gcgtccatgc gagaattcta tcacgagtca gggctagcga aagtccccag
     1321 ctgggaacat attgtctatg acaagtaatt attagtaata gaacccactc cctggaggcg
     1381 gtatgccgtt cggtgctttt atcaacgcga tcccgatgcc ggtgttcctg gtgatccatc
     1441 tcgccgcgtt cgcgatcggt gcgttcttcg cgaagaaggc gttcggcggc ggggcgtccg
     1501 agttcggctg gggcttcacc ctcttcgcgc tcgccgagat cagctatctg acctatcacc
     1561 tcgactggac cgtgttcctc ttcgcccaca cgatctccga ggtactcgac cttctggcgt
     1621 tcatcatgat cttcgccggc gccgcgaagt ccgtcctggc gcgcaagtga cccgttccgt
     1681 ttcgcggggg ttacggatgg gtgcggtggc actcgtcgtg gccatctcgg gggcctgtca
     1741 tcgggccccc cccgcacccc cggtggccac gacgcgggtg gagctgccgc cttcgtacct
     1801 gttcgcgccc gccgacatcg tcgtgcacgc cggtgcgacg gtggtctgga ccaaccatga
     1861 ccactttacg cactccatcc acctgacgga cgacggcggc acctcgatga tggtcccgcc
     1921 gggggacagc gtgcgcttca cctttacccg gcccgggttg catcactacg actgctcact
     1981 gcacccgcat gacatgcatg ggacggtcct cgttcagggc gccttgcggg actccacttc
     2041 cggcggcata tccgggtctc catgaaacgc tttcagcacc tgatcgtcgg tggtggcatg
     2101 acggctgcgg ccgcggctca tggggcccgt gaacaggcac ccaaggcgag cgtcggcatc
     2161 atcagcgccg agtcacaccc gccctacaac cggcctccgc tgtcgaaggc cctgtggaag
     2221 ggcgaccccg agtcgagcat ctggcgcacc agtgatgtgg agggcgtgga ggtgatcctc
     2281 tcccgtcggg tgaccgccat cgaccccgcg aagaagacgg ccaccgacga tcacggcggg
     2341 acctacggct atgagacctt gctcctggct acgggcggcg ctccccgccg tctggccggc
     2401 gcccccgaca ccatcgttta cttccgtacg ctcgacgact accgtgccct gcacgcggcg
     2461 gccgctccgg gcaagcattt cgtggtcctg ggcggtggat tcatcggctc ggaggtcgcg
     2521 gcggccctgc gcatgcgggg ggcagcggtc acgatggtga ttccggaggc cggggttggt
     2581 gcacgggtgt ttccggcgcc gctctcgcaa aatctggtcg agtactaccg gagcaagggc
     2641 gtgacggtcc gcaccggcga gggcgtcgcc accgtgtcgg agaatgccgg gaaggcggtg
     2701 gtgaaaacca cgaccggcct cgaaattccg gccgacggcg tcgtcgccgg cctcggcatc
     2761 atcccctcca tcgagctggc ccaggctgcc ggactcaaga ccgacaacgg catcgtggtc
     2821 gacgagtacc tgcggacctc caacccctcg atctacgccg cgggggacgt cgcgaacttc
     2881 ccgtccaagg cgctcggcca ccgctcgcgg gtagagcatg aggacaacgc caacgcgcag
     2941 gggcgccagg caggccgcaa cctcgccggc gcgaacgagc ggtacgacca cctccccttt
     3001 ttctacgggg atctgttcga gctgggatat gaggcggtgg gggacctgga tgcgcgcctc
     3061 gaaacggtca gcgactggaa ggcacccttc cgcgagggcg tcgtctactt cctcggcgag
     3121 ggcagggtga agggcgtgct gctctg
//
LOCUS       DASUBP010000118         9820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_629891, whole genome shotgun sequence.
ACCESSION   DASUBP010000118 DASUBP010000000
VERSION     DASUBP010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9820
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_629891"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..527)
                     /locus_tag="VFU75_04425"
     CDS             complement(165..527)
                     /locus_tag="VFU75_04425"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU5466110.1"
                     /translation="MPRYFTFIRAAENQGAPPKALMDAMENFIGESLQDGSLVSTGGL
                     KLSPHGVRIRVGKGKLTMTDGPFAESKEVIGGYAILEAPTKAKVLEVARKFMQLHVDH
                     WPDWEGESEIREIDFIAP"
     gene            complement(530..919)
                     /locus_tag="VFU75_04430"
     CDS             complement(530..919)
                     /locus_tag="VFU75_04430"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU5466111.1"
                     /translation="MRFLMMVIPKAYETAKEDFVPPAELMIKMGKFNDSMSKAGVLLS
                     LDGLTHPAKGARVSFGGGKSRVTDGPFAEAKEIVGGFWMIQVRSREEAIEWARRAPMQ
                     DGDIIEIRQVQELADFPDDLKKAIGVK"
     gene            complement(1030..1869)
                     /gene="nadC"
                     /locus_tag="VFU75_04435"
     CDS             complement(1030..1869)
                     /gene="nadC"
                     /locus_tag="VFU75_04435"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEU5466112.1"
                     /translation="MAEAVRLGWDEVRRALEEDRAAADETTRLLGAETGKLGTLRFVA
                     EGRFVVAGVPVVGEVFAQLDERTHLGPGIPEGLWAHPGDSLATVKGPAGVLLSGERVA
                     LNFLQRLCGVATLTRRAVDAVQGKAKITHTRKTTPGLRTLERYAVLAGGGVENRSSLA
                     DAIMWKDNHWELLAAGGQSLSDVLKQAPRGLPVIVEVETEAQLEDALAAGVKHLLIDN
                     QPPDQFAKWARRAGPDVTIQASGGITVENVAAYAQAGAHLISMGALTHSAPAVPIKCE
                     LVK"
     gene            complement(1862..2812)
                     /gene="nadA"
                     /locus_tag="VFU75_04440"
     CDS             complement(1862..2812)
                     /gene="nadA"
                     /locus_tag="VFU75_04440"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HEU5466113.1"
                     /translation="MSRRRDLIAEIRELKTARGAAILAHNYQRPEIQDLADHVADSLA
                     MARRAAMLDAPVLVICGVHFMAETAAIANPDKRVLIPDLHAGCSLAETIQAADVRAWK
                     ATHPDGVVVAYVNTAAAVKAEADYCCTSGNAVAVIRALPPRVPVLFLPDFFLGQYLKR
                     VTGRELDVWMGECHVHAGLTREALDRRRSEYPDAEFLVHPECGCVTQAMYYAEAEGDV
                     AAGRMQIVSTEQMMTRARESTASRYVVATETGVLHRLRRENPGKEFVAAVETAECRFM
                     KLITLEKLRDSLRDLKHEVTVPPAVAAKARRAIDRMLAIG"
     gene            3058..3549
                     /locus_tag="VFU75_04445"
     CDS             3058..3549
                     /locus_tag="VFU75_04445"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU5466114.1"
                     /translation="MERINYARVAPGAVHAMNGLGNYLKSSGLEPGLLDLVKLRASQI
                     NECAYCVDMHWKDARAAGETEQRLYGVSVWRESPYYTDRERAALAWAEALTRIAEDEI
                     SDELYAETRKQFDEKALVDLSLAIVEINGWNRLAKTFRSVPGSYQPAARPAGAPAATS
                     VKG"
     gene            complement(3546..6173)
                     /locus_tag="VFU75_04450"
     CDS             complement(3546..6173)
                     /locus_tag="VFU75_04450"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5466115.1"
                     /translation="MSIWRQFQRGLDVLLHRDTADRELSDELEHYVAEAAAAYVEQGL
                     SPADARRRARMDAGSLASVQEEVRSGGWESMVEAVVTDVRFAFRRLMGSPGFTFVTAL
                     TLALGIGATTAIVSAVTPILFRPLPYPHAERLAAVVEDHKGVRGAGTFALYHAFADQT
                     HSFEALAVTRRWQPTLTGAGRPDRLSGERVSHEYLRVLGVSPLRGRDFTETEDRLNGP
                     RVVVVSDGLWRGRLSADPAILGKSLMLDGDPYLVIGVMPPGFQDVEDPAAELWAPLQY
                     DMSQGTAWGHHLRTVGRLRPGVGIEAATADVAGVGRAVLTQQHPETYDPNTDFMVTSL
                     QGELTRGVRPALLTIVGAVILVLLIALVNVTNLLLARGVQRRTEFALRAALGAGRGRL
                     VRQLLTESLVLAAVGGALGIAVAAVGVKTIVALSPPGLPRADAIAVNGTVLFVGALVT
                     TLVGLAVGILPALHATRTDPQQEIQHGSRRTAGGHRRARGILVAAEVALALVLLVGSG
                     LLLRSSARLFAVDVGFDQGRMLTMQVQLSGHRFDDDSSIVRFFTQALDAVRTVPGVTA
                     AGFTTQLPLSSDDDEYGAHFDAEPNRPAATYDVFRYAVTPGYLETLGVPLLSGRSLGD
                     ADRAGSPLVAVISASLAHDRFGAEDPLHQQLRLGPAGPYTIVGVVGDIHQASLAQSQG
                     EAVYLPVGQWPFPDRSMSLVLRGRGASALLAPAVEGAVWSVDPDQPIVRVATMSDLVA
                     RTAGERRFALTLFEVFALAALGLAAAGIYGVLSGSVNERTRELGIRSALGASRAEVVR
                     MVVSQGATLTGIGAALGLIAATGLSRLIASLLFETPPVEPVTYLTVTALLFVVALVAC
                     SVPAVRASRVDPVEALRAD"
     gene            complement(6178..6513)
                     /locus_tag="VFU75_04455"
     CDS             complement(6178..6513)
                     /locus_tag="VFU75_04455"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU5466116.1"
                     /translation="MDEPNTDVIPGTLDLLILKTLSLEPMHGFGIARRVEQISRAVFK
                     VNPGSLLTALTRLERSGALDAEWRVTDSGRRAKYYSLTKSGQKQLEAATDDWTRRASA
                     VARLLRAEG"
     gene            6635..7714
                     /locus_tag="VFU75_04460"
     CDS             6635..7714
                     /locus_tag="VFU75_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU5466117.1"
                     /translation="MPGPNVERRAFVRHAATLTLLAPGLGAALVSCRTDEAHHQTPGS
                     NASTGTPTPNPDSKLDTTLAPAPAGVTPAPDLTAVRYRRVDPALPPAPSDHRLAVHFH
                     AREALVRLSDDTVVAAWTFEGDVPGPRLHCHVGDTVDFTLTNDVAMPHSMDFHAAQID
                     PKTAFRSALKGQSVSYRFTPKYPGVFLYHCGTAPVLLHLGSGMFGAIVVSPREPLPRA
                     REFVLVQSEFYLGPAAHGVRPFAYDRMLSTLPDFVTFNGRPDQYGREPLRAKVGERVR
                     FWVANAGPTHPCAFHVVGEQFDTVYLGAPPGNAIHGVQTWSVPAGGGMCFELQADVAG
                     EFAVVNHSFGHGQKGAIGRLVVEEA"
     gene            7714..8085
                     /locus_tag="VFU75_04465"
     CDS             7714..8085
                     /locus_tag="VFU75_04465"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5466118.1"
                     /translation="MSPVDRPMEGDVLSFHLDEEIRALKAQMGTGRDRLGHTLVKEGP
                     LRVTLVTLGPQGSINPHTADGAVAIQVLEGSISLDVAGTTKHLETGDLVTLAPGIQHA
                     VASATGATFLLTLTAVLPGRG"
     gene            complement(8082..9503)
                     /locus_tag="VFU75_04470"
     CDS             complement(8082..9503)
                     /locus_tag="VFU75_04470"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HEU5466119.1"
                     /translation="MTGLRDFLEMPYDELEERNLAAKKQRQDRVPADQIQEERLKYLR
                     DEKRIKAVTVCFTDLEGRFHMLDYDKKFLLGSVDNLTFDGSSIRGFSRQQESDLRLRV
                     DWPAFYWLPSDIFGHGKVLAFADVLEREGAAYPADIRGVLKEYAAKLFTKDGTECFAA
                     NEIEGFLFQGRDAERHYHETGRFDYLSTGGYYHSLPGDALRMFIDTAAEVQRAMGFAN
                     EKDHPEVAPSQFEMNYSYSEACIAADQVQLYKLLSRQVAARMDMTACFLPKPVTGVNG
                     NGMHTNVSVARKGKNQFWDPKGQDQLSAAGWDFTRRILSNGAEICLILNSSVNAYRRL
                     DPHFEAPNQIKASAIDRGSMIRIPLGNERSKRVEVRSIAPDANPYLALYSVFRTGLEG
                     PLAPDAGAQSKYLSDNIYDAIRLYQGSAFYKQMLGAEVHGKFADLKLAQADRCPRALG
                     SVVKAAEIQFHHEVTNQHLWSQF"
     gene            complement(9584..>9820)
                     /locus_tag="VFU75_04475"
     CDS             complement(9584..>9820)
                     /locus_tag="VFU75_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466120.1"
                     /translation="DLVLRQGMRPAYLGVALGLGSAVVLTRFLRAILYEVAPLDWVTF
                     GLVPLLLLGVALGSVLIPAVRASRVAPVEALRME"
BASE COUNT         1659 a         3406 c         3199 g         1556 t
ORIGIN      
        1 gcggcggcga agggcgatgg gggtcagaag caatgcgccg agtgccaccc gcgcccaggc
       61 tacgaacgcg ggtggcactc ctccatccac cgccactttg atgaagagat agggaatccc
      121 ccagatgacg gagctgaggg caaacaggac ccaggctttc ctggtcacgg agcgatgaaa
      181 tcgatctcgc ggatttcgct ttccccttcc cagtcgggcc agtggtcgac gtggagctgc
      241 atgaacttcc gcgccacctc gagaaccttc gccttcgtgg gagcttcgag aatcgcgtag
      301 cccccgatca cttccttgga ctcggcgaac gggccatcgg tcatcgtgag cttccccttc
      361 ccgacccgga tccggacacc gtgcgggctg agtttgagcc cgccggtcga gaccaatgat
      421 ccatcttgca gggactcgcc gatgaaattc tccatcgcat ccatcagggc cttcggtggg
      481 gcaccctggt tctccgccgc tcggatgaac gtgaaatagc gtggcatggt cacttcaccc
      541 cgatcgcctt cttgaggtcg tcaggaaagt ccgcgagctc ctggacctga cggatttcga
      601 tgatgtctcc atcctgcatc ggtgcccgcc gtgcccactc gatcgcttct tcccgcgagc
      661 ggacctggat catccagaag ccacccacga tctcctttgc ttcggcgaac ggaccgtcgg
      721 tgacccgcga cttgccaccg ccgaagctca cccgggcacc cttggcgggg tgggtcaatc
      781 cgtcgagtga gagcaggaca ccggccttgc tcatcgagtc gttgaacttt cccatcttga
      841 tcatgagctc ggcgggcgga acgaaatctt ccttcgcggt ctcataggcc ttcggaatta
      901 ccatcatcag gaagcgcatt gggaacctca ggggtcaggg gtcaggggtc agccggtcca
      961 tccggcttcc actccaggac gaatgggagg tcgggatttc gacaaacttc gtaacatgtt
     1021 gtggcttaat tacttgacta gctcgcactt gatcgggacc gcgggggcgg aatgcgtcag
     1081 ggcgcccatt gagatcagat gggcgccggc ctgcgcgtag gccgcgacgt tttcgaccgt
     1141 gatgccccca gatgcctgaa tcgtgacgtc cggtcctgcc cgtcgagccc acttcgcgaa
     1201 ttggtccggg ggctggttgt cgatcaacag gtgcttcacc ccggccgcca acgcgtcttc
     1261 caattgggct tcggtctcga cctccacgat caccggcaga ccgcgcgggg cctgcttcag
     1321 cacgtcggag agtgactggc caccggccgc cagcaactcc caatgattgt ccttccacat
     1381 gatggcgtcg gcgagcgaac tccgattctc cacaccgcct cccgccagga ccgcgtaccg
     1441 ctcgagggtc cggaggccgg gggtcgtctt gcgggtgtgc gtgatcttcg cctttccctg
     1501 cacggcgtcg accgcccggc gtgtgagcgt cgcgacgccg cagagccgct gcagaaagtt
     1561 cagcgcgacc cgctctcccg agagcaacac gccggccggc cccttcaccg tggccagcga
     1621 gtcacccggg tgagcccaca ggccttccgg gatgccgggt ccgagatgcg tccgctcgtc
     1681 gagctgtgcg aacacttcac cgaccaccgg gacacccgcc accacgaacc gcccctcagc
     1741 gacgaaccgg agcgtgccca gcttcccggt ctcggccccc aacaaacgcg tggtttcgtc
     1801 ggcggcggca cgatcctcct cgagggcccg tcgcacctcg tcccacccca aacgaaccgc
     1861 ttcagccaat ggcgagcatc cggtcgatgg cccgccgggc cttcgctgcc accgcagggg
     1921 gcacggtgac ttcgtgcttc aggtcgcgga gggaatcccg gagcttctcg agtgtgatga
     1981 gcttcatgaa gcggcactcc gccgtctcga cggccgccac gaattccttg ccggggttct
     2041 cccggcgcag gcggtgcagg acgcccgtct cggtagcgac gacgtaccgg ctggccgtgg
     2101 actcccgggc ccgcgtcatc atctgctccg tcgagacgat ctgcatccgg cccgccgcca
     2161 catcgccctc cgcctcggcg tagtacatcg cctgggtgac gcagccgcac tccggatgca
     2221 ccagaaactc cgcatcggga tattcggagc gccgtcgatc cagtgcctcg cgggtcagcc
     2281 ccgcatgcac gtggcattcc cccatccaga cgtcgagctc gcggcccgtg acgcgcttca
     2341 gatactggcc caggaagaag tcggggagaa agagcacggg gacccggggc gggagcgccc
     2401 ggatcacggc caccgcattg cccgacgtac agcagtaatc ggcctccgcc ttcactgccg
     2461 cggccgtgtt gacgtaggcg acgaccaccc cgtccggatg cgtcgccttc cacgcgcgca
     2521 catcggccgc ctggatcgtc tcggcgagcg agcagcccgc atggaggtcg ggaatcagga
     2581 cgcgcttgtc cggattggcg atcgccgccg tctcggccat gaaatgaacg ccgcagatca
     2641 cgagcaccgg agcgtccagc atcgccgccc gccgggccat ggccagcgag tcggcgacat
     2701 ggtcggccag gtcctggatt tcgggacgct ggtagttgtg ggccagaatc gcggcgccac
     2761 gcgccgtctt gagctcccga atctcggcga tcagatcacg ccggcgggac atcgtcgtgt
     2821 cggaaatcac tgccctgccc cattggtagc ggtaccaggg ctggggggcc gattgggaaa
     2881 cgcctcggtc cgaaggggtt cggaccgctc ccagcctggg gcacgctgac ctcacgcgcc
     2941 tgcccaactc taacggcggg tgcgtccgag agggaggggg ccggaccggg gaacctccgg
     3001 cacagctgtc ccattagaat gcagacggca acctcaatag agagaaggga ttcacatatg
     3061 gaacgcatca actacgcccg ggtggcaccc ggcgccgtgc acgccatgaa cggccttggc
     3121 aactacctga agagctcggg cctcgaaccc gggctgctcg acctggtcaa gctgcgcgcg
     3181 tcacagatca acgaatgcgc gtattgcgtg gatatgcact ggaaggacgc ccgggccgcg
     3241 ggcgaaaccg agcagcgcct ctatggggtc tcggtatggc gcgagtcccc gtactacacg
     3301 gatcgagagc gcgccgcgct ggcgtgggcg gaggcactta cccgcatcgc cgaagacgag
     3361 atctcggacg agctgtatgc ggagacgcgg aagcagttcg acgagaaggc cctcgtcgat
     3421 ctgtctctcg ccatcgtcga gatcaacggg tggaaccggc tggccaagac cttccggtcc
     3481 gtgccgggga gctaccagcc cgccgcccgc cctgccggag ccccggcagc cacgagcgtg
     3541 aaaggctagt cggcccgcag tgcctcgacg ggatccacgc gggacgcgcg caccgccggt
     3601 accgagcacg ccaccaaggc caccacgaac aggagggccg ttaccgtcag gtaggtgacg
     3661 ggctccaccg gcggcgtttc gaacaacagg ctggcgatca accgcgagag tcccgtcgcc
     3721 gctatcaagc cgagcgctgc accgattccc gtcagcgtcg ctccctggct caccaccatc
     3781 cggacgacct ccgcgcgcga ggcgccgagc gccgaccgaa tccccaactc acgggtccgt
     3841 tcgttgacgc tccccgacag cactccgtaa atccccgcgg cggcgaggcc gagtgcggcc
     3901 agggcgaaca cctcgaagag cgtgagcgcg aagcggcgct ctccggccgt acgcgcgacg
     3961 aggtcgctca tggtcgccac gcggacgatc ggctggtccg ggtccacgga ccacaccgcg
     4021 ccctcgaccg cgggagccag cagcgcggac gcgccacgac cccggagcac gagcgacatc
     4081 gagcggtcgg ggaaaggcca ctggccgact gggaggtaca ccgcctcccc ctgcgactgc
     4141 gcgagcgagg cctgatgaat gtcccccacg accccgacga tcgtgtaggg ccccgcaggt
     4201 cccagcctca attgttggtg cagagggtct tccgccccga atcgatcgtg ggcgagcgag
     4261 gcactgatca cggcgaccag cggagaaccg gcccggtcgg cgtcgccgag ggaacggccg
     4321 ctcaagagcg gtacgccgag cgtctcgagg tatcccggcg tgacggcgta gcggaacacg
     4381 tcgtacgtag cggcgggccg gttcggttcg gcgtcgaaat gcgccccgta ttcgtcgtcg
     4441 tcgctcgaca gcggaagctg tgttgtgaac cccgccgcgg tgacgcccgg gaccgtacgg
     4501 acggcgtcca gcgcctgggt gaagaacctg acgatggagc tgtcatcgtc gaatcgatgg
     4561 ccggacagct ggacctgcat cgtgagcatc cggccctggt cgaacccgac gtccacggcg
     4621 aagagccgcg cggaggatcg gagcaacagc cctgagccca ccagcaggac cagagccaga
     4681 gcgacttccg cggccaccag aatgccccgc gcgcgccggt gcccgcccgc cgtcctgcgg
     4741 gagccgtgct ggatctcctg ctgggggtcg gtgcgtgtcg cgtggagcgc cgggaggatg
     4801 cccacggcaa ggccgacaag cgtcgtgacc agggcgccga cgaacagaac ggtgccgttc
     4861 acggcgatgg cgtcagcccg ggggagtccc ggtggactca acgccacgat ggttttcacg
     4921 cccacggccg caaccgcaat gccgagcgcg ccaccgacgg ccgcgagcac cagactctcc
     4981 gtgaggagtt gccggaccag ccggccccgc ccggcaccca gggccgctcg caaggcgaac
     5041 tcggtccggc gctggactcc gcgcgccagc aggagattgg tcacgttcac cagggcgatg
     5101 agcaggacca gaatcacggc gccgacgatc gtgagcaggg cgggacggac cccacgggtc
     5161 agctcaccct gcagcgaagt gaccatgaag tcggtgttcg gatcgtacgt ctcggggtgt
     5221 tgctgagtga ggacggcgcg ccccactccc gcgacgtccg cagtcgcggc ctcgatgccc
     5281 acaccgggcc ggagcctccc gaccgtcctg aggtgatgtc cccaagccgt tccctgcgac
     5341 atgtcatact gaagcggagc ccacagctcc gccgcgggat cttctacgtc ctgaaatccg
     5401 gggggcatga cgccgatcac cagatagggg tcgccgtcca gcatcagcga cttccccagg
     5461 atggccggat cggcggacag cctcccgcgc cacagcccat cgctgacgac cacgacacgt
     5521 ggcccgttga gccggtcctc cgtctcggtg aagtcgcgcc ccctcaacgg ggagacgccg
     5581 agcacccgca ggtactcgtg actcacccgc tcccccgaga gccggtcggg ccgcccggct
     5641 ccggtgagcg tgggttgcca ccgcctggtc accgcgagcg cttcgaagga gtgggtctgg
     5701 tcggcgaacg cgtggtagag cgcaaacgtc cccgcccccc tcacgccctt gtggtcctcg
     5761 accacagcgg cgagccgctc ggcgtggggg tacgggagtg ggcggaagag aatgggcgtg
     5821 acggcgctca cgatggcggt cgtcgcgccg atgcccaggg ccagagtcag cgcggtaacg
     5881 aaggtgaaac cgggagatcc catcagccgg cggaacgcga agcggacgtc ggtcaccacg
     5941 gcctcgacca tgctctccca cccgccggag cggacctctt cctgaacgct cgccaacgag
     6001 cccgcgtcca tccgggcccg ccgccgcgcg tccgccgggc tcagcccctg ctcgacgtac
     6061 gccgccgcgg cttccgcgac gtagtgctcg agctcatccg agagctcccg gtccgcggta
     6121 tcgcgatgga gcaggacgtc gaggccacgc tggaactgcc gccagatcga cacgcggtca
     6181 cccctccgcc cggagcagac gggccacggc ggacgcgcgt cgcgtccagt cgtcggtggc
     6241 ggcttccagc tgcttctggc ccgatttggt cagggagtag tacttggcgc ggcgcccgct
     6301 gtccgtcact cgccactcgg cgtcgagggc accggagcgc tcgagccgcg tgagggcggt
     6361 cagcaaggaa ccgggattga ccttgaatac ggcgcgcgag atctgctcca cccggcgggc
     6421 gatcccgaac ccgtgcatgg gttcgagaga aagcgtcttc agaatcagga ggtcgagggt
     6481 gccggggatg acgtcggtgt tgggttcgtc cacaggaggc tccggtactc ggtttccccc
     6541 atggtacgcc cattcctctt gactgtcaag atgtgacggt caagggttgg cgaggcacgc
     6601 tagattcttg cgcacacccc tctggagacc ccccatgccg ggcccgaacg tcgaacgccg
     6661 cgccttcgtc cgtcacgcgg cgaccctcac cctgctggca ccgggactgg gcgccgcgct
     6721 ggtatcctgt cgaaccgatg aagcacacca tcagacaccc ggctcgaatg cgtccacggg
     6781 cacccccact cccaacccgg acagcaagct cgacaccacg ctggcccccg ctccggccgg
     6841 cgtcaccccc gcgccggatc tcacggccgt gcgctatcgc cgggtggacc ccgccctccc
     6901 gccggcaccg tccgaccacc ggctcgcggt tcacttccac gcccgcgagg ccctggtacg
     6961 actctcggac gataccgtgg tggccgcctg gacattcgaa ggtgacgtcc cgggcccccg
     7021 gctgcactgc cacgtgggag acacggtcga cttcacgctc accaacgacg tggccatgcc
     7081 tcattcgatg gacttccacg ccgcgcagat cgaccccaag accgcgttcc gatcggcgct
     7141 caaggggcaa tccgtctcct accgcttcac gcccaagtac ccgggcgtgt tcctgtacca
     7201 ctgcggcacc gccccggtgc tcctgcatct tggatcgggc atgttcggcg ccatcgtggt
     7261 gtcgccgcgt gaacccctgc cccgggcgcg ggagttcgtg ctggtccaga gcgagttcta
     7321 cctggggccg gccgcccacg gggtgcgccc cttcgcctac gaccggatgt tgtcgacgct
     7381 cccggacttc gtgaccttca acggccgccc cgaccagtac ggacgggagc cgctgcgggc
     7441 caaggtgggt gagcgggtcc gtttttgggt cgccaatgcg gggcccaccc atccctgcgc
     7501 ctttcacgtc gtgggcgagc agttcgatac cgtgtacctc ggcgccccgc cggggaacgc
     7561 catccacggg gtgcagacct ggagcgtccc tgccggcggc gggatgtgct tcgagctcca
     7621 ggccgacgtg gcgggggagt tcgccgtcgt caaccactca ttcggacatg gccagaaggg
     7681 cgccatcggg cgcctcgtgg tcgaggaggc ctgatgtcac ccgtcgatcg accgatggaa
     7741 ggcgacgtcc tgtcgttcca cctggatgag gagattcgcg cgctcaaggc ccagatgggg
     7801 acaggccggg accgcctggg ccataccttg gtgaaggaag gccccctgcg ggtcacgctg
     7861 gtgacgctcg ggccccaagg ctccatcaac ccgcataccg ccgacggcgc cgtggcgatt
     7921 caggtgctgg agggatcgat ctcgctggac gtcgcgggga ccacgaagca tctggaaacg
     7981 ggggacctgg tgacgctggc gccgggaatc cagcacgccg tcgcctccgc caccggggcc
     8041 acgtttcttc tcacgctgac cgcggtcctg cccggccgtg gctagaactg cgaccagagg
     8101 tgctggttcg tgacctcgtg gtggaactgg atctcggcgg ccttcaccac cgaccccagg
     8161 gcgcggggac accggtcggc ctgggcgagc ttcaagtccg cgaacttgcc gtggacctct
     8221 gccccgagca tctgcttgta gaacgcgctg ccctgataga gccggatcgc gtcatagatg
     8281 ttgtccgaca gatacttcga ctgggccccg gcatccggcg cgagtggccc ttccagcccg
     8341 gtgcggaaca ccgagtacag cgcgagatac gggttggcgt cgggcgcgat ggagcggacc
     8401 tcgacacgct tcgagcgctc gttgcccaac gggatccgga tcatcgagcc gcgatcgatg
     8461 gctgaggctt tgatctgatt cggcgcctcg aagtgcgggt ccagacgccg gtaggcgttg
     8521 acgctcgagt tcaggatcag acagatctcg gcgccattcg acaggatgcg ccgggtgaag
     8581 tcccaccccg ccgccgacag ctggtcctga cccttcgggt cccagaactg gttcttgccc
     8641 ttgcgcgcca ccgaaacgtt ggtgtgcatg ccgttcccgt tcacaccggt gacgggcttg
     8701 ggcaggaagc aggcggtcat gtccatccgg gccgcgacct ggcgggacag gagcttgtac
     8761 agctgcacct gatccgccgc gatgcacgcc tcggaatagc tgtagttcat ctcgaactgc
     8821 gacggcgcga cttccggatg gtccttctcg ttggcgaacc ccatcgcccg ctgcacttcg
     8881 gccgcggtgt cgatgaacat ccggagcgcg tcgcccggca gcgagtggta gtacccgccc
     8941 gtggagaggt agtcgaacct gccggtctcg tgatagtgcc gctccgcatc gcgtccctgg
     9001 aacaggaacc cttcgatctc gttcgcggcg aagcactccg tcccgtcctt cgtgaacaac
     9061 ttcgccgcgt actccttgag cacgccccgg atgtcggccg gataggcggc accctcgcgc
     9121 tccaggacgt ccgcgaacgc gaggactttg ccgtggccga aaatgtcgga gggcagccag
     9181 tagaacgcgg gccagtcgac gcgcaggcgc agatcggatt cctgctggcg ggagaagccc
     9241 cggatggacg agccgtcgaa ggtgaggttg tccaccgacc ccaggaggaa tttcttgtcg
     9301 tagtccagca tgtgaaaccg gccttcgagg tccgtgaagc acaccgtcac ggccttgatg
     9361 cgcttttcgt cccggaggta cttcaggcgc tcttcctgga tctggtcggc gggcactcga
     9421 tcctgccgct gcttcttggc cgccaggttc cgttcttcaa gctcgtcgta aggcatttcc
     9481 aggaagtcgc gcaggccggt catcaagatc ctcgattctg aagggaaggg acgccccagg
     9541 ggtcagggaa cgttctccac ttccggcgga gcccgcaagt tgctcattcc atacggagcg
     9601 cctccaccgg agccacgcgc gacgcccgca ccgccgggat caggaccgag ccgagagcga
     9661 cgccgagaag caacagcgga acgagtccga aggtcaccca gtcgaggggc gctacctcat
     9721 agaggatggc ccgcaggaac cgcgtgagga ccaccgcgct tccaagtccc agcgcgaccc
     9781 cgagataggc tgggcgcatc ccctgccgca gcaccagatc
//
LOCUS       DASUBP010000119         3196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_85191, whole genome shotgun sequence.
ACCESSION   DASUBP010000119 DASUBP010000000
VERSION     DASUBP010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85191"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1826
                     /locus_tag="VFU75_04480"
     CDS             <1..1826
                     /locus_tag="VFU75_04480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU5466121.1"
                     /translation="DPAGNILLVNRQACEILGYPEAELLGQSVDLLLPVRLRAGHVSQ
                     RAAYLRAPATRQMGAGRELQALRKDGREIVVEVGLAPVLQDGQTYVIASIVDVTKRKE
                     TQSRLEWLSLAVEQSPIAVIMTDLDGQIQYVNAAFTRNTGYQPDEVLGKTPRVLKSGE
                     TSPTVYRDLWATIRAGKVWHGDLVNRRKDGTLYEDAMWVFPVTDSKGQVVRILALKED
                     VSRKRSLEAQLRQAQRMESIGRLAGGVAHDFNNILTAIFGYTELVSETFAPGSPQRED
                     LEEIRKASQRASALTRQLLAFSRQQVLQPVVVKTNELVENLEKMLRRIVSEDIKLRLA
                     LSKATGNVRVDPGQFDQVLMNLVVNARDAMPKGGSLVIETANAELDEQYAMEHQPVVP
                     GPYVLIAVSDTGGGIPPEVRSRIFEPFFTTKEIGKGTGLGLSTVYGIIKQSGGYVWVY
                     SEMGRGTTFKIYLPRVDAPADTSEFAAPPKELKGTETILLAEDDETLRSLTTGILRKL
                     GYVVLAAATPDMARAIARDHHGPIHLLLTDVVMPGSSGRDLARELAKSRPQTKVLFVS
                     GYTGEAMIQHGLTGPGLNYLQKPYTPSVLAAKVREVLSQQS"
     gene            complement(1835..3031)
                     /locus_tag="VFU75_04485"
     CDS             complement(1835..3031)
                     /locus_tag="VFU75_04485"
                     /inference="COORDINATES: protein motif:HMM:TIGR02913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466122.1"
                     /translation="MRTALLILSALAALGCADQQPLTPAALGATAAAATRRDVTAPRY
                     RIDKLPILAGTTQGAGISNRGWVTGYSVATDGLRHAVLWDGNGVTDLGQPGLISLPQW
                     PGISSSGAVAGITQITTPDTLGESWSCAAFMGTAGKVCLGFAYYGGTMHTLPTWGGEN
                     GFATGVNARGDVVGWAETAVHDPTCNAPQVLQFRATVWSTHDASQRQLRPYAGDSTSA
                     ATAINDRGQVVGISGDCDVAVGRKSARHALMWDGDQVIDLGDLHGQYWNTPMSINANG
                     SMVVGFTNPPDGDLDGDSLRAFVWTRATGMRDLGRLPGDQSSQALGVNMAGQIVGLSC
                     GDVTCYGLLWQNGQMYRVQDLAGPDFPDQIFSAKDINESGQITGRMIDHVTKKAVAYV
                     ATPITN"
BASE COUNT          590 a         1018 c         1056 g          532 t
ORIGIN      
        1 cggatccggc gggcaacatc ttgctcgtga accgtcaggc ctgcgagatc ctcggatatc
       61 cggaagctga gctcctgggc cagtcggtcg atctgctgct gccggttcgc ctacgcgccg
      121 ggcacgtcag ccagcgagca gcgtacctgc gggcccctgc cacgcgccag atgggcgctg
      181 gccgggagct ccaggcgctc cgaaaggatg gccgggagat cgtcgtcgaa gtggggctcg
      241 cgccggtcct gcaggacggt cagacctacg tgatcgcgtc catcgttgac gttaccaagc
      301 gcaaggaaac ccagtctcgg ctcgaatggc tgtcactcgc cgtagaacag agcccgatcg
      361 ctgtcatcat gacggacctc gacggacaga tccagtacgt gaatgcggca ttcacccgaa
      421 ataccggata ccagcccgac gaagtgttgg gaaagacgcc ccgcgtactg aagtccgggg
      481 agacctcacc caccgtgtac cgggatctgt gggccacgat ccgcgcgggc aaggtctggc
      541 acggcgatct ggtgaaccgc cggaaggatg ggacgctgta tgaggacgcg atgtgggtgt
      601 tcccagtgac ggacagcaag ggacaagtcg tccggatcct ggccctcaag gaggacgtct
      661 ccaggaagcg ctctctcgaa gcccagttgc gccaggcgca acgcatggag tccatcggac
      721 gcctggccgg gggtgtggcg cacgacttca acaacatcct gaccgccatc ttcggttaca
      781 ccgagctggt gagcgaaacg ttcgctccgg gctcccctca gcgggaagat ctcgaggaga
      841 tccgcaaggc gtcgcagcgc gcgtcagcgc tcacccgcca gctcctcgcc ttcagccgcc
      901 agcaggtgtt gcaaccggtc gtcgtgaaga cgaatgagct ggtcgagaac ctggaaaaaa
      961 tgctccgtcg catcgtcagc gaagacatca agctgaggct ggccctcagc aaggccaccg
     1021 ggaatgtccg ggtcgatccg ggtcagttcg accaggtgct gatgaacctc gtcgtaaatg
     1081 ctcgtgacgc catgccgaag ggcggctcac tcgtgatcga aaccgcgaac gcggaactcg
     1141 acgagcagta cgccatggag caccagccgg tggtcccggg cccctatgtg ctcatcgccg
     1201 tgagtgacac cgggggcggt atcccgcccg aggtgcggag ccggatcttc gagcccttct
     1261 tcaccaccaa ggagatcggc aaggggacag gtctcggact ctctaccgtt tacggcatca
     1321 tcaagcagtc gggcgggtac gtgtgggtct acagcgagat ggggaggggg acgacgttca
     1381 agatctactt gccacgggta gacgccccag ccgacaccag tgagttcgcc gccccgccga
     1441 aggagttgaa ggggacggaa acgatcctat tggctgagga tgatgagacg ctccgcagtc
     1501 tgacgacggg gattctgcgg aagctgggat acgtggtcct ggcggcggcc acgccggata
     1561 tggcacgggc catcgcgcgt gaccaccacg gtcccatcca ccttctcctg acggacgtcg
     1621 tcatgccggg ttcgtcaggc cgcgacttgg cgcgcgagct cgccaagtca cggccccaga
     1681 cgaaagtcct cttcgtctca gggtatacgg gcgaagccat gatccagcat ggcctcaccg
     1741 gaccaggcct gaactacctc cagaagccgt acacgcccag cgtgctcgcc gcgaaggtgc
     1801 gtgaggtgtt gtcccagcaa tcctgaggcc gcggtcagtt ggtgatcggt gtggccacat
     1861 acgccacggc cttcttcgtc acatgatcga tcatccgccc cgtgatctgt cccgattcgt
     1921 tgatgtcctt ggctgaaaaa atctgatcgg ggaagtcggg gcccgcgagg tcctgcaccc
     1981 ggtacatctg cccattctgc cacagcaggc cgtagcaggt cacgtcaccg caggaaagcc
     2041 ccacgatctg accagccatg ttgactccca acgcctggct cgactgatcg ccgggcagcc
     2101 tgccgaggtc ccgcatgccg gtcgcgcggg tccagacgaa cgcccgcaga ctgtcgccgt
     2161 caaggtcccc atcgggtgga ttcgtgaaac ccactaccat cgacccgttg gcgttgatcg
     2221 acatcggcgt gttccagtac tggccatgga gatcacccag gtcgatgacc tgatcaccat
     2281 cccacatcag cgcgtgccgg gcgctcttcc ggccgaccgc cacgtcgcag tctcctgaaa
     2341 tgccgaccac ctggccgcga tcgttgatgg ccgtggcggc ggacgtcgaa tcgccggcgt
     2401 agggacggag ctggcgctgc gaagcgtcgt gcgtgctcca gacggtcgcg cggaattgga
     2461 gcacctgtgg agcgttgcac gtcgggtcat gcacggccgt ctcggcccag ccgaccacgt
     2521 caccgcgggc gttcaccccg gtcgcgaagc cgttctctcc gccccacgtc ggcagagtgt
     2581 gcatggtgcc gccgtagtag gcgaagccga ggcacacctt tcccgcggtg cccatgaagg
     2641 cggcacaact ccacgactcg ccgagggtgt cgggcgtcgt gatctgtgtg attccggcga
     2701 ctgcaccgct ggatgaaata ccgggccatt ggggcagact gatcaggccg ggctggccga
     2761 gatcggtgac accgttgccg tcccagagga cggcgtggcg cagtccgtcc gtggccaccg
     2821 aatagccggt cacccaaccg cggttgctga tgccggcgcc ctgcgtggta ccggcgagaa
     2881 tgggaagctt gtcgatgcgg tagcgggggg cggtgacgtc ccgccgggtc gcggcggcgg
     2941 ccgtggcgcc gagcgccgcc ggggtgaggg gctgctgatc cgcgcagccg agggcggcga
     3001 gcgcggacag gatcaacaaa gcagtgcgca tgaggacgct cctgagaggg gaaggatcac
     3061 ggaacgtcgg actatccccc cgccgcagcg ccactgcttg ccggattggg acgcagaatc
     3121 tggaacggac gggccactca tcgcagagaa gtggccgttg gtctccggaa tgacgtggtt
     3181 ggcgtccctt tcatct
//
LOCUS       DASUBP010000120         5315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_398245, whole genome shotgun sequence.
ACCESSION   DASUBP010000120 DASUBP010000000
VERSION     DASUBP010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5315
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_398245"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /gene="lptC"
                     /locus_tag="VFU75_04490"
     CDS             <1..375
                     /gene="lptC"
                     /locus_tag="VFU75_04490"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HEU5466123.1"
                     /translation="LADTSYLYNSTQRAEMKNVHVTFYDATGRETSTLTSREGTLRNA
                     GDMEGRGNVVVVRASDDGTLRTEKIDFNQRANQVSSDQPFTFDTPQQHLKGVGFTSDP
                     DFKDLKAQRVTGTGGKFVLPNQ"
     gene            372..1778
                     /locus_tag="VFU75_04495"
     CDS             372..1778
                     /locus_tag="VFU75_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466124.1"
                     /translation="MIVATLALLALQQPAVPAGPRDTSAGRPCVLEIDSLARVHQISS
                     GADTNVYMAGGVLAHCKGSGTSFRSDSAAYYGGVKRWDMVGRAHVVDTALTLDANQII
                     YYLRQGRLDAHYNVVAVNRENHSTLRGPNLTYYRAVQGVRDTTETYATQRPTIDYRGA
                     GASDTSEPYVIVADRFRSKGNDRMWWGGTVTVDRSDLSARGDSMSMDQTAGLGILVGH
                     PSVTGKGSQNYTLTGTRIEMGLSGREMNAIRALGNGRAAGTDWTLTADTIHIRLADRK
                     LQQAFAWGPKDSVRARAVSSTTTMVGDSLAMDTPNQTISEARSYRHAHSTSKRDSTTA
                     DSLADWMSGDTLLAHWIQAPDSTGKQRSVLSKVVARGSGKAFTHSYSATPDSARPRLI
                     QRNPRDSTKMDTIPDTRPSLNYTTADLITVDMAKGRVQQVTAIGHINGFNLDPVPVKD
                     TTKTDSTKAHARAPVKKP"
     gene            1775..2947
                     /gene="lptB"
                     /locus_tag="VFU75_04500"
     CDS             1775..2947
                     /gene="lptB"
                     /locus_tag="VFU75_04500"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HEU5466125.1"
                     /translation="MIDDLLNSRDPSVPLAVVALHTTADEDGTGYFGPLAVAYRDAYL
                     HARAATAGETGESHTLSVDDARHNLSASVLPRLALLGVVSVPKGELHGDTAITFTPAM
                     WRELSADPAGIAARLRQSAERALKSVESPSGPHAAALGGSKLEAANLMKTYKGRRVVN
                     DVSLAVQQGEIVGLLGPNGAGKTTSFYMITGLIRPDDGLIRLDGRDITREPMYKRARS
                     GIGYLAQEPSVFRKLTVEENVLAILETMNLEAAERATRLESLLDDLAIKHLRRQRAFS
                     LSGGERRRLEITRALVTQPKFMLLDEPFAGVDPIAVHDIQTIVAGLRHRGIGVLITDH
                     NVEQTLDIVDRAYIMFEGKVQVAGTVRDLVFDDRVADLYLGPTLTARLRARLGEAA"
     gene            2944..4407
                     /gene="rpoN"
                     /locus_tag="VFU75_04505"
     CDS             2944..4407
                     /gene="rpoN"
                     /locus_tag="VFU75_04505"
                     /inference="COORDINATES: protein motif:HMM:TIGR02395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEU5466126.1"
                     /translation="MKTGMHQHASLQQTLKMNPRLYQAMDLLYMPLLDLQQHLKQELL
                     SNPFLEMAEAEEESPLDATQVETKPEKNEESGDDEPDWENILLDDGAQGLAAPRDLSP
                     AREYVEPVAPTTRDLSDHLQDQVQMLDLTPRQQLMADEFLGNIAEDGYLGASLEQIVA
                     GANQLLAEHADAQNFGGEAPWFTMAEAEEMLHIIQHLDPPGVGARDLRECLLLQLEDQ
                     GQQESLVYKLVRDAFDDLIAHRWSELGKRFGLEPAEVQKVADDLSKFDPKPGLQYASA
                     SDSYIVPDLIVEKIGDRYQVFLNDGGLPRLRIASTYRDIARDKNKYQGENKDFIQQRL
                     NSANWMIQAIEQRRQTMLKVMNFIVDRQREFFEKGVEFLRPLTLREVADVISMHESTV
                     SRVTNEKYVQTPRGLLPLKFFFSSGLTTTTGEDASARSIKAQIQKLVTDEDTKEPLTD
                     QQIVEIFAQRGVKIARRTVAKYRDQLHILPARMRKRL"
     gene            4404..>5315
                     /locus_tag="VFU75_04510"
     CDS             4404..>5315
                     /locus_tag="VFU75_04510"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5466127.1"
                     /translation="MSLAMPAAAPEVSILVPAKDEAENLPELVKQVREAMVGQPYAAE
                     LVIVNDGSTDHSAETLKELAAKHGFLQVVTHRSRRGIADALRSGADAARGNILVFWPA
                     DLQYLPADIPALVAPIIANEADIVTGTKQGHYEKRFVSWVYNSLCRILFGVRVTDLNS
                     VKAYRRDVVDAVPTRPDWHRFMVVIAAVQGYRVTSRPVPVYPRRAGKSKFGISRIPVG
                     VLDLLSVWFQLRFGRKPMLFFGVSGAVLVALGVLVGIYAIIERYVFGIGFRPWLYLVM
                     LLVLAGLVLFGFGFVGELVAGMREELRA"
BASE COUNT          967 a         1702 c         1804 g          842 t
ORIGIN      
        1 ctggccgaca cgagctacct gtacaacagc acccaacggg ccgaaatgaa gaacgtgcac
       61 gtcacgttct atgacgccac agggcgggaa acctccacgc tgaccagccg cgaggggacg
      121 ctccggaacg ccggggatat ggagggacgc ggaaacgtcg tcgtggtccg cgcctccgac
      181 gacggcacgc tgcggacgga gaagatcgat ttcaatcagc gggccaatca ggtgagctcc
      241 gatcagccgt tcacgttcga tacgccccaa cagcacctca agggtgtcgg cttcacgtcc
      301 gaccccgact tcaaggatct gaaggcgcag cgggtcaccg gcaccggggg caagttcgtg
      361 ctccccaacc aatgatcgtg gcgaccctcg cgctgctcgc cctgcagcag cccgccgtgc
      421 cggcgggtcc gcgcgacacg tccgcggggc gcccctgcgt gctggagatc gactccctcg
      481 cccgggtgca ccagatcagc tccggggccg atacgaacgt gtacatggcc ggaggcgtgc
      541 tggcgcactg caagggaagc gggacgtcgt tccggtcgga cagcgccgcg tattacggcg
      601 gggtgaagcg ctgggacatg gtgggccgcg cgcacgtcgt ggataccgcg ctcacgctcg
      661 acgccaatca gatcatctat tatttgcggc aagggcggct ggatgcgcat tacaacgtcg
      721 tcgccgtgaa ccgggagaac cactcgaccc tccggggtcc gaacctcacg tattaccggg
      781 ccgtgcaggg cgtgcgggac acgaccgaga cctatgcgac ccagcgtccg acgatcgatt
      841 atcgcggagc cggcgcgagt gacaccagtg aaccctatgt gatcgtggcg gaccgttttc
      901 ggtccaaagg gaacgaccgc atgtggtggg gaggcaccgt caccgtcgac cggtcggacc
      961 tgtccgcccg cggcgattcg atgagcatgg accagacggc agggctcggc attctcgtcg
     1021 gccatcccag tgtgaccggc aaggggtcgc agaactacac cttgaccggt accagaatcg
     1081 agatgggact gtcggggcgg gagatgaacg ccatccgggc tctggggaac ggccgggcgg
     1141 cggggacgga ctggaccctg accgccgaca cgattcacat ccggctggcc gaccggaaac
     1201 tccagcaggc gttcgcctgg gggccgaagg attccgttcg cgcccgggcc gtctcgtcga
     1261 ccacgaccat ggtcggagac tcgctcgcga tggatacgcc gaaccagacc atctccgagg
     1321 cccggagcta ccggcacgcc cactcgacgt ccaagcggga ctcgacgacg gccgactcgc
     1381 tggcggactg gatgtcgggg gacacgctgc tggcgcattg gatccaggca ccggattcga
     1441 cggggaagca gcgctcggtg ctgagcaagg tggtggcgcg cgggagcgga aaggcgttca
     1501 cccactcata ctcggccacg cctgacagcg cgcgtccccg gctcatacag cggaacccgc
     1561 gcgactcgac gaagatggat acgattcccg acacccggcc gtccctgaac tacaccaccg
     1621 ccgatctgat cacggtggac atggccaagg ggcgggtcca gcaggtcacc gccatcgggc
     1681 acatcaacgg cttcaacctg gacccggttc cggtcaagga cacgacgaag acggactcca
     1741 cgaaggcaca cgcgcgtgct ccggtgaaga agccatgatc gacgacctgc tgaacagccg
     1801 ggatccctcg gtcccgctcg cggtggtcgc gctccacacg accgctgacg aggatgggac
     1861 cggatacttc ggccccctcg cggtcgcgta tcgggacgcc tatctgcatg cccgggcggc
     1921 gaccgcggga gaaaccgggg agagccacac gctctccgtg gacgacgcgc gtcacaacct
     1981 cagtgcttcg gtcctgccgc gtctcgcctt gctcggcgtc gtctcggtgc cgaagggtga
     2041 gttgcacggt gacaccgcga ttacgttcac gccggccatg tggcgggagt tgtcggccga
     2101 tcccgccggg atcgcggcac gcctccgcca gagcgccgag cgggcgctca agtcggtgga
     2161 gtcaccgtcg ggccctcatg cggcggcgct gggggggtcg aagctcgaag ccgcgaacct
     2221 gatgaagacg tacaagggcc ggcgcgtcgt gaacgacgtc tcgttggcgg tgcagcaggg
     2281 cgaaatcgtg ggactgctgg gtccgaacgg ggcggggaag accaccagct tctacatgat
     2341 cacggggctg atccgtcctg atgacggcct gatccggctt gatggacgcg acatcacgcg
     2401 cgaacccatg tacaagcggg cgcggtccgg catcggctac ctggcgcagg agccctcggt
     2461 cttccgcaag ctcacggtcg aagagaacgt gctcgcgatt ctcgagacca tgaatctgga
     2521 ggcggcggag cgggccaccc gtctcgagtc gctgctcgac gatctggcga tcaagcatct
     2581 ccggcggcaa cgggctttct cgctgtcggg cggcgagcgg cggcgtctcg agatcacccg
     2641 ggcattggtg acgcagccca agttcatgct cctggacgag ccgttcgcgg gcgtcgatcc
     2701 catcgcggtc cacgatattc aaaccatcgt ggccggtctt cggcaccggg gcataggcgt
     2761 attgatcacg gaccacaacg tggaacagac gctggacatc gtcgatcggg cgtacatcat
     2821 gttcgaaggc aaggtacagg tggccggaac ggtacgggac ctcgtgttcg acgaccgggt
     2881 ggccgatctc tacctcggcc cgacgctgac ggcgcgcctc cgcgcgcgac tgggtgaggc
     2941 ggcatgaaga ccggaatgca tcagcacgcg tcgctgcagc agacgctgaa gatgaatccg
     3001 cgcctgtatc aggcgatgga tctcctctac atgcccttgc tcgatctgca gcagcacctg
     3061 aagcaggagc tgctctcgaa tccgttcctc gagatggcgg aagccgaaga ggagtccccg
     3121 ctcgacgcga cgcaggtcga gaccaagcct gaaaagaacg aagagtcggg ggacgacgag
     3181 ccggattggg agaacatcct gctcgacgac ggggcccagg gactcgcggc gccgcgcgat
     3241 ctgagccccg cgcgagagta tgtcgagccg gtggcgccga cgacccgcga tctctccgat
     3301 cacctccagg accaggtcca gatgctcgac ctgactcccc ggcaacagct catggccgac
     3361 gagttcctgg ggaacatcgc ggaagacggg tacctgggcg ccagcctcga acagatcgtg
     3421 gccggcgcga atcagctgct ggcggagcac gcggatgcgc agaatttcgg tggggaagcg
     3481 ccgtggttta ccatggccga ggcggaggag atgctgcaca tcatccagca tctggacccg
     3541 ccgggggtag gggcgcggga cctccgcgaa tgcctgctgc tgcagctcga ggatcagggg
     3601 cagcaggagt ccctcgtcta caagctggtg cgggatgcct tcgacgacct gattgcgcac
     3661 cggtggagcg agctgggcaa acgcttcggc ctggagcccg ccgaggtgca gaaggtggcg
     3721 gacgacctgt cgaagttcga tcccaagccg ggcctgcagt acgcgtccgc gagcgactcg
     3781 tacatcgtgc ccgatctgat cgtcgagaag atcggggacc gctatcaggt gttcctgaat
     3841 gacggcggct tgcccaggct ccggatcgcc agcacctacc gggacatcgc ccgggacaag
     3901 aacaagtacc agggtgaaaa caaggacttc atccagcagc gcctgaacag cgccaactgg
     3961 atgatccagg ccatcgagca gcgccggcag accatgctga aggtcatgaa cttcatcgtg
     4021 gaccggcagc gggagttctt tgagaagggc gtcgagttcc tgcgtcccct cacgttgcgg
     4081 gaggtcgcgg acgtcatctc gatgcacgag agcacggtga gccgcgtgac caacgagaag
     4141 tacgtccaga cgccgcgggg cctcttgccg ctcaagttct tcttctcgag cgggctcacc
     4201 accacgacgg gagaagacgc gagcgcgcgg tccatcaagg cgcagatcca gaagctcgtg
     4261 accgatgagg acaccaagga gcccctgacc gatcagcaga tcgtcgagat cttcgcccag
     4321 cggggtgtga agatcgcccg gcgcaccgtc gccaagtacc gggatcagct gcacatcctg
     4381 ccggcacgga tgcggaagcg tctgtgagcc tggccatgcc ggccgccgcg cccgaggtca
     4441 gcattctggt gccggccaag gacgaggcgg agaatctccc ggagctggtc aagcaggtcc
     4501 gggaggccat ggtgggccag ccatacgccg ccgagctcgt gatcgtcaat gacggctcga
     4561 cggaccactc ggcggagacg ctgaaggagc tcgccgcgaa gcacgggttt ctgcaggtgg
     4621 tgacccatcg ctcgcggcgc ggcatcgcgg acgcgctccg ctccggcgcc gacgcggcgc
     4681 gcggcaacat cctggtcttc tggcccgccg acctgcagta cctgcccgcg gacattccag
     4741 ccctggtcgc gccgatcatc gccaacgaag ccgacatcgt gaccggaacc aagcagggcc
     4801 actacgagaa gcggttcgtc tcgtgggtct acaattcgct ctgccggatc ctgttcggcg
     4861 ttcgcgtcac ggacttgaat tcggtgaagg cgtaccgtcg tgacgtcgtg gacgcggtac
     4921 ccacgcggcc cgactggcac cggttcatgg tggtgatcgc ggcggtgcag gggtatcgcg
     4981 tgacctcgcg gcccgtgccg gtctaccccc ggcgcgcggg caagtcgaag ttcggcattt
     5041 cgcggatccc ggtgggcgtt ctggatctcc tgtccgtctg gtttcagctc cggttcgggc
     5101 gcaaaccgat gctcttcttc ggcgtctcgg gcgcggtgct ggtcgccctc ggcgtgctcg
     5161 tgggcattta cgccatcatc gagcgttacg tattcgggat cggtttccgc ccgtggttgt
     5221 atctcgtgat gctcctggtg ctcgccggcc tcgtgctctt cggcttcggg ttcgtgggag
     5281 agctggtggc ggggatgcgg gaggagctcc gggcc
//
LOCUS       DASUBP010000121         2774 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_657289, whole genome shotgun sequence.
ACCESSION   DASUBP010000121 DASUBP010000000
VERSION     DASUBP010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2774)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2774)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2774
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657289"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1967)
                     /gene="fusA"
                     /locus_tag="VFU75_04515"
     CDS             complement(<1..1967)
                     /gene="fusA"
                     /locus_tag="VFU75_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEU5466128.1"
                     /translation="MPRTTPLDKIRNIGIMAHIDAGKTTTTERILYYTGRNYKLGEVH
                     DGTATMDWMEQEQERGITITSAATTAFWTRFGIPYRINIIDTPGHVDFTVEVERSLRV
                     LDGAVTVLDAVGGVEPQTETVWRQADRYRVPRIIFVNKMDRVGADFDRCLAMVKDRLG
                     AKAIPIHLPLGSGELFTGLIDVVRQVEIVYDDETLGKKYVEGPVPEALKERIAAMRHE
                     LLEMIVEHDDAILHKYLEGQALTEEEIRAVLRKATINGGVVPVLCGAAFKNKGVQALL
                     DAVVDYLPAPVDVAAIQGHLPHHDETHADRKASDDEPFAALAFKIMTDPYVGRLTFFR
                     VYSGVLAAGSYVYNSTKDRKERIGRLLQMHANKREEIEEVRAGEIAAAIGLKDTRTGD
                     TLSDENHPIILEAMKFPEPVISVAIEPKTKADQDKLGVALQKLSEEDPTFRVHSDAET
                     GQTIISGMGELHLEIIVDRMKREFKVEANVGRPQVAYRETVRKRVEYVEGKFIRQSGG
                     RGQYGHVVIHLEPGKAGTGFVFEDNIVGGVIPKEYINPIEAGVKEALENGVLAGYPMV
                     DVKVELVDGSFHDVDSSEMAFKIAGSMAVKEAARQAHPILLEPVMDVEVVTPSDYMGD
                     VIGDLSARRGKIGGMTQRADAQIVAASVPLA"
     gene            complement(2145..2615)
                     /gene="rpsG"
                     /locus_tag="VFU75_04520"
     CDS             complement(2145..2615)
                     /gene="rpsG"
                     /locus_tag="VFU75_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007406077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEU5466129.1"
                     /translation="MSRRKKAVRRPVPPDPRYDSQTVSKFLNNLMGAGKKSIAERVFY
                     NAMDLIEQRTGQPGVNVFKQALSNAKPALEVKSRRVGGATYQVPVEVRPERRTALAMR
                     WLITYSRSRREKDMSERLAAEMILASKGEGETIKKKEDTHKMAEANRAFAHYRW"
     gene            complement(2612..>2774)
                     /locus_tag="VFU75_04525"
     CDS             complement(2612..>2774)
                     /locus_tag="VFU75_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006863839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEU5466130.1"
                     /translation="QEHSIVMIRGGRVKDLPGVRYHIIRGTLDSAGVTDRKQSRSKYG
                     AKRPKAGGK"
BASE COUNT          408 a          918 c          892 g          556 t
ORIGIN      
        1 tcggccagcg gcacgctggc ggccacgatc tgcgcgtcgg cgcgctgcgt catgccgccg
       61 atcttcccgc ggcgggccga caggtcgccg atgacgtcac ccatgtagtc cgacggcgtg
      121 acgacttcga cgtccatcac cggctcgagc aggatggggt gcgcctgacg cgccgcctcc
      181 ttcaccgcca tcgaacccgc gatcttgaac gccatttcgc tcgagtccac gtcgtggaac
      241 gagccgtcca ccagctcgac cttcacgtcc accatcggat agccggccag gacgccgttc
      301 tcgagggctt ccttcacgcc cgcctcgatc gggttgatgt attccttggg aatcacgccg
      361 cccacgatgt tgtcctcgaa cacgaagccg gtgccggcct tgccgggctc gaggtggatg
      421 accacgtggc cgtactggcc gcggccaccg gactggcgga tgaacttgcc ttcgacgtac
      481 tcgacgcgct tccggacggt ctcgcggtag gccacctgcg ggcggccgac gttggcttcg
      541 accttgaact cccgcttcat ccggtcgacg atgatttcga ggtgcagctc gcccatcccc
      601 gaaatgatgg tctggccggt ttcggcgtcc gaatgcacgc ggaacgtggg gtcttcttcg
      661 gaaagcttct ggagggccac acccagcttg tcctggtcgg ccttggtctt gggctcgatg
      721 gcgacggaga tgacgggctc cgggaacttc atcgcctcga gaatgatcgg atggttctcg
      781 tccgagaggg tgtcgcccgt gcgcgtgtcc ttcaggccga tcgcggccgc gatctcgccg
      841 gcccggactt cctcgatctc ctcacgcttg ttggcgtgca tctggaggag acggccgatg
      901 cgttccttgc ggtccttcgt gctgttgtag acgtacgacc cggcggccag gacgcctgaa
      961 tagacgcgga agaacgtgag gcgtccgacg taggggtcgg tcatgatctt gaacgccagg
     1021 gccgcgaagg gctcgtcgtc cgaagccttg cggtcggcat gcgtttcgtc gtgatggggg
     1081 aggtgaccct ggatcgcggc cacgtccacc ggcgcgggca ggtagtccac caccgcgtcg
     1141 agcagggcct gcacgccctt gttcttgaag gcggcgccgc agagcaccgg gaccacgccg
     1201 ccgttgatcg tcgccttgcg gaggacggcg cggatctctt cctcggtgag ggcctgaccc
     1261 tcgagatact tgtggagaat ggcgtcgtcg tgctcgacga tcatctccag gagctcgtgc
     1321 cgcatggcgg cgatgcgctc cttcagggcc tccgggaccg ggccttcgac gtatttcttg
     1381 cccagcgtct cgtcgtcata aacgatctcg acctgccgaa cgacgtcgat cagaccggtg
     1441 aacagctcgc cggagccaag cggcaggtgg atcggaatgg ccttggcgcc cagccggtcc
     1501 ttcaccatgg cgaggcagcg gtcgaagtcg gcgccgacgc ggtccatctt gttgacgaag
     1561 atgatgcggg ggacgcggta ccggtcggcc tggcgccaga ccgtttcggt ctgcggctcc
     1621 acgccgccca cggcgtccag cacggtcacg gcgccgtcga ggacgcggag cgaacgctcc
     1681 acttcgacgg tgaagtccac gtgaccgggg gtgtcgatga tgttgatgcg gtagggaatg
     1741 ccgaagcggg tccagaatgc cgtcgtggcc gcggaggtga tggtgatccc ccgctcctgc
     1801 tcctgctcca tccagtccat cgtggccgtg ccgtcgtgca cttcgcccag cttgtagttc
     1861 cggccggtgt aatacaggat ccgctcggtc gtagtcgttt tcccggcatc gatgtgggcc
     1921 atgatgccga tgttgcggat cttgtcgagt ggcgttgtgc gcggcatagc ttctctgaat
     1981 ccctcttcct tcagcaaaaa tgcggggcga ccgtgtcgcc cgggattccc gggccggtct
     2041 cgctccgctc acatccggga gacgagcctc cctggatggg ctgcgtatgt gtggtcttcc
     2101 ctacttagcg acttagcgac ttagcgactt agcttcttag cgtcttacca ccgataatgc
     2161 gcgaaggccc ggttggcctc agccatcttg tgcgtgtctt ccttcttctt gatcgtctcg
     2221 ccctcaccct tcgaggcgag aatcatctcc gccgccaacc gctccgacat gtccttctca
     2281 cggcgcgacc ggctgtacgt gatgagccag cgcatggcca gcgcggtccg gcgctccggc
     2341 cgcacttcga ccggcacctg gtacgtcgcg ccgccgacgc ggcgggactt cacctcgagg
     2401 gccggcttgg cgttcgacag cgcctgcttg aacacgttca cgcccggctg cccggtgcgc
     2461 tgctcgatca ggtccatcgc gttgtagaac acgcgctcgg cgatcgactt cttgcccgcg
     2521 cccatcaggt tgttcaggaa cttcgacacc gtctggctgt cgtaccgcgg atcgggcgga
     2581 acgggacggc gaacggcttt cttccggcgg ctcacttgcc acctgccttc ggacgcttgg
     2641 caccgtactt ggagcgcgac tgcttccgat cggtcacccc ggccgagtcc agcgtgccgc
     2701 ggatgatgtg ataccgcacg cccggcagat ccttcacgcg gccgccgcgg atcatcacga
     2761 tcgagtgctc ttga
//
LOCUS       DASUBP010000122        20932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_561557, whole genome shotgun sequence.
ACCESSION   DASUBP010000122 DASUBP010000000
VERSION     DASUBP010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 20932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20932
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_561557"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2958)
                     /locus_tag="VFU75_04530"
     CDS             complement(<1..2958)
                     /locus_tag="VFU75_04530"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5466131.1"
                     /translation="MKFFEVLSRQRRVIYLAVALLTAAGAWFAVGLPSAIYPELAFHR
                     IQVVVEGSALGARQMVFSVTRPLEEAISIVPGVVRVDSRTIRGATETSVYFDPHTDMT
                     QALSLTRARVNQVEPTLPAGLDVQIELMTPSLFPILSYNLEGGDPATLYDIARYQIRP
                     VLSRIPGVGRVIVQGSDVHEVEVVADPGRLAALGLTYDGLAADIRQAITVQAVGRVSR
                     DYKQYLVVTDQEARSTEDVGAVVLPNGLRVRDVATVSLGTEDHVRIIAGDGKPAAQIS
                     LTRQLGGNTLDIADSATAALRALAPELPPGVQVKAIYDQAALVRDAVRSVRDAMIIGA
                     ALAVIILLLFLRHGRITAISATAIPLTLIITVAGMRALGQTFNLMTLGAMAIAIGLVI
                     DDAVVITENIARHLALTSDRRLAIREALQELLWPVTTSTITTVVVFLPLGLLTGVVGQ
                     FFAALAATLTCAVLVSLGLAFTIIPLLAEQFVTAHEAAPENDRSFLGRVHAGLTRLTD
                     WYGRTLAAALHHPRRLVFTLLAVAATGLAGYRLVGTGFLPEMDEGAFVLDYFTPGGTA
                     LAETDREVRIAEGILARTPEIVGTSRRTGAEMGLFATLQNSGDLVARLKPQGERPRDI
                     FAVMDAVRDSIGAAVPRLHIEFVQILSDVINDLAGAAKPVEVKVFGENLDTLEAYARR
                     LQSPLEKIEGLEDLYNGVSEPTAELLLKVREGEAARMGLTPAEVGDAVSGALLGADAG
                     EVHAEDRPIGVRVRAPDAVRFDIARLASVPLLASGATRSTPLGAVADFLPVDSRAELL
                     RENQQQMIDINADVSGRSLGDVSRDVRRVLADEPPPAGVRVVLAGQYANQQEAFGALV
                     IVLALASLSVVGVMVVQFESFVEPLIVLLVAPVSFTGGVALLLVTGTALNVSSMMGFI
                     LLVGLIVKNGIILLDFTRLRMRRDGLNLEAALRDAARVRLRPILMTTLCTLFGLLPLA
                     LGLGAGSELQRPLA"
     gene            complement(2955..3995)
                     /locus_tag="VFU75_04535"
     CDS             complement(2955..3995)
                     /locus_tag="VFU75_04535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5466132.1"
                     /translation="MKRLVTLLVLIAACHGGGHEADQPAAAPVATATVRQGPFTEIVT
                     AIGTVAPRPGRYAELAAPAPTRVARILVTPGQRVAVGDTLVVFEQASFDAAAHAADVA
                     LAAAQHAADRASRLVTAGILPRKDADQAELDLAAAQTAAVTARRNAQLAALQSPLAGV
                     VTRLTAVLGGPADPSEPVVAVADPDALDLLFALSVDQAARVRPGDSVLVMAGDQVGGD
                     TLGAGRVRDVGAAVDPASRTITVRAHLSRATRALRIGEAVRGRIAVLTVPRAVVIPEA
                     ALVPNGEGYDVFIVDSTGIAHRTPVVLGGRSDSLAEIRSGVAPGQTVVTTGAYGITDS
                     ARVAPVARPAKP"
     gene            complement(3992..5185)
                     /locus_tag="VFU75_04540"
     CDS             complement(3992..5185)
                     /locus_tag="VFU75_04540"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU5466133.1"
                     /translation="MRPVLVFLLLPCSGLSAQTPRAVSRADAVAFALAHGPRAATARA
                     DSAAAYGDLAAARAFANPSFSATYTKSVPRYHYIFDIPIDWPWLRAPRVGAAGAGAEA
                     ARARFTFEVAAVRFDADTAYTVALAAQAHARLSHRTAGDADSLLRVARLRRDAGDASE
                     MDTRLAEINAGELANTAAEDSLAAVAALVNLQLVMGLPGDSAVVLSDSLQFPDTTGDQ
                     IPSFPLPVLAAQAELTAAERSLTLAKSVNLLSPSFQVGVERGDPTGSEPGALPTFGFA
                     LPLPLFNWNGGAITRARAERDAAQIRLDATRRAAAAERARAVGERSVALTRAHRDAGL
                     VASADSVAAMFLQAYGEGAVSLPEVLEAQRNAREALGRYVDDLALANTAVGLVKLLDA
                     NREHP"
     gene            complement(5223..6491)
                     /locus_tag="VFU75_04545"
     CDS             complement(5223..6491)
                     /locus_tag="VFU75_04545"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5466134.1"
                     /translation="MKPLTRLRLQLTLWYAGVLMLILLVLGFGLFRAIRHQISRQLDL
                     SLEAAAHSLEQAARVREVERAQTRGPVADAVEELHIPDRTLYFFDSAGGAIIPSTVPP
                     EMASVARRAAQTGSVNLTIDGPGDHDTRVHAERFTGGTGTRYVALATADRVELEDQYA
                     SLIETLAAGALGALVLVAAGGWFLVRQSTAPIERSMDRMRRFMADAAHELRTPVTILR
                     TRADLALDQARDPDRDTALLKAVAREAGRIGAIVGDLLTLARADAGERPLARERVFLD
                     DLAADTVNDLRPLADRRGVTVAVGSFEECAVRGDPVPLRQLLRILLDNAIKFTPAGGR
                     VRIDASVEKGTPTVRVVDTGIGIPADQLPHVFERFYRGEQARRAGEGNEGAGLGLAIA
                     RWIADAHQAKIDISSNGSGTEVVVRFPAEV"
     gene            complement(6488..7153)
                     /locus_tag="VFU75_04550"
     CDS             complement(6488..7153)
                     /locus_tag="VFU75_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5466135.1"
                     /translation="MRVLVVEDDPQLAALLREGLREQRIDPVLATTFADGLERGRGTD
                     FDVVILDVRLPGGTGFDLCRRLRDEGVAAPVLMLTALDAVDDRVRGLEAGADDYLTKP
                     FDFRELVARIKALARRRPSVTPAVHKIADLTVDLSARTVSRAGKAITLTVKEFALLEL
                     FVLHAGAVVDRAAITAHVWDDNHDPFANVLDVLIRRLRAKIDDPFTPKLIATVRGAGY
                     RLG"
     gene            complement(7208..8536)
                     /gene="hemL"
                     /locus_tag="VFU75_04555"
     CDS             complement(7208..8536)
                     /gene="hemL"
                     /locus_tag="VFU75_04555"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: protein motif:HMM:NF000818.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HEU5466136.1"
                     /translation="MGRRRPGAPLPAGARLTPSRSALLFERAQKLFPGGVNSPVRAFR
                     AVGGQPVFLARGSGATVEDVDGRSYIDYVLSWGPLILGHAHPMVVRAVQEAALEGTSF
                     GAPTPREVELGELIRTQMPALELMRFVNSGTEAVMSALRVARAFTGRSKILKFEGCYH
                     GHADALLVKAGSGVATLALPDSPGVPSLTAAETLVVPYNDLPAVDRIMVEYGKNIAAV
                     IVEPVAGNMGVVPPEPGFLGGLRDLTRGAGALLIFDEVMTGFRAHPSGVQGMTGVTPD
                     LTTLGKIIGGGLPVGAYGGRREIMELVAPAGPVYQAGTLSGNPLAMAAGLATLTELGR
                     PGVWDSIAKTTADLVEGLNGAARSARVPCVVNRVGAMFTLFFTDHPVRSWDEARRADV
                     ARFGAFFRSLLQHGVYWVPSQFEAAFLSAAHGQGEIDRTVEAAGRALRVI"
     gene            complement(8488..9855)
                     /gene="hemG"
                     /locus_tag="VFU75_04560"
     CDS             complement(8488..9855)
                     /gene="hemG"
                     /locus_tag="VFU75_04560"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HEU5466137.1"
                     /translation="MTRVAVIGGGIAGLAAAQRLVEARVSCTVLERDHRPGGKIVSQS
                     VGGFLVEGGPDCVLSSKPGAVALARSLGIDGLLHGTDPAHRGTFVKRQGRLHPLPEGI
                     TGLVPSRIASLLTTRLLSPTGRIRAGLEAFVPRGGGGDESIAQFASRRFGLEAYQWVV
                     EPLLSGVYAGDGEQLSLSATFPQLVRAERDAGGVLRSTWRKRGVPQGERGGFLSFPDG
                     VEHLVRATVARLPAQTLRLGVGARSVTTEGDGFRIRCDDGATDTFDQVIIATPAHVAA
                     ELVEDLNPSLAIELSRIPFVSSATVSLGYADAPAFGGSGYVCPRAEGGAAVAVTWSSN
                     KYQGRAPAGGALVRVFLGRAGDEGWVDEADDALIAEARRELEKVCGVVQKPVLARVFR
                     WSRGLPQYVIGHLDRMERIAAWLHGLPGLHVAGASYRGVGIPDCITSGWAAADQVLRS
                     LRVPA"
     gene            complement(9852..10781)
                     /gene="hemH"
                     /locus_tag="VFU75_04565"
     CDS             complement(9852..10781)
                     /gene="hemH"
                     /locus_tag="VFU75_04565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEU5466138.1"
                     /translation="MSEPFGVLVMAYGGPDALSDVEPYLLDVRGHRPTPGHVVEEVRR
                     RYAAIGGRSPILERTRAQAGRLEEALAARGRPVPVAVGMRHWKPRIATGLEELRARGV
                     EHVVGLVMAPHYSRMSIELYFRKVDEELKALSFVGIADWHLLPGYVAAAADRVRDALE
                     RFPADRRHEIPVIFSAHSLPRRILESGDPYPDQLAASMRAIVARIGNGGLRHQFAFQS
                     AALTPDPWLGPDVGTVLKDLADAGERNVVVAPIGFTCEHVEVLYDLDVELRCQAGELG
                     VHLERAGMVNDDPLMMAGLADLVLDTARREGWS"
     gene            complement(10778..11815)
                     /gene="hemE"
                     /locus_tag="VFU75_04570"
     CDS             complement(10778..11815)
                     /gene="hemE"
                     /locus_tag="VFU75_04570"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HEU5466139.1"
                     /translation="MTAPSTSTLLAAARREPGRPTPVWFMRQAGRYLPEYRAIREKHS
                     LLEICANPQLATEITLQPVWRLGVDAAIVFADILLPLVPMGLDLAFVEGEGPVLRNRI
                     RGPADVARLVDTNVANALAPVAETVRLVRGALPPTIPVIGFAGAPFTVASYAIEGGSS
                     RHFVETKRLMYEHPDAWNALLTKLVRVLGEYLAAQVGAGADIIQVFDSWVGVLNADDY
                     RRYVLPHTRALFTQLGLLGVPVIHFGVNTGELLPLLVQAGGDVIGLDWRIGLDDGWRR
                     IGFDRGVQGNLDPVALFAPPAELEARVHDIIARAEGRPGHIFNLGHGILPTTPVDAVR
                     RVVDLVHTLTA"
     gene            complement(11812..12501)
                     /locus_tag="VFU75_04575"
     CDS             complement(11812..12501)
                     /locus_tag="VFU75_04575"
                     /inference="COORDINATES: protein motif:HMM:NF018484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chlorite dismutase family protein"
                     /protein_id="HEU5466140.1"
                     /translation="MAEDTHRTFNHFSLLRFDAQYWRQEPKARRQAVCAALEALRGGV
                     DALHLYQTFGVEAGTDLLVWTAVDDGDPAVPARYFPHLGRALAPIREFATLTTALWGF
                     TQPSQYTKTKSRQEIDPFTAERSPYLVMYPFTKTSDWYQKSREERRDMMLEHIKLGKQ
                     YEDITQLLLYSTGLQDQEFVVVYETADLTRFSNLVTELRSTQGRPFTERDTPLHAAVH
                     QADWGTASQWL"
     gene            complement(12505..13506)
                     /gene="hemB"
                     /locus_tag="VFU75_04580"
     CDS             complement(12505..13506)
                     /gene="hemB"
                     /locus_tag="VFU75_04580"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HEU5466141.1"
                     /translation="MIRLRRLRASPGLRNLVRETRLDPAQLIYPLFVTAASGEPRAIA
                     SMPGVSQWPVAQIAAQAEAVHRAGLGAILLFGIPRTKDATGTGSFDDHGVIQDAVRAI
                     KAAVPDLPVITDVCLCEYTEHGHCGVLNGAGKGRVHPELPEGYVLNDESVEILKRVAV
                     SHARAGADVVAPSAMLDGMVSGIRAALDQNSFSHIPVLSYSAKFASAFYGPFREAGGG
                     APKQGDRQSHQLDIANGREALRESAVDVDEGADLLMVKPALAYLDVVRETRMRFPDHP
                     LVAYNVSGEYAMVKAAAERGWLDERRAVLEHLTAMRRAGADLIITYHARDAARWLTE"
     gene            complement(13503..14246)
                     /locus_tag="VFU75_04585"
     CDS             complement(13503..14246)
                     /locus_tag="VFU75_04585"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HEU5466142.1"
                     /translation="MTGPRVLVTRPRAQSRDLVTALESWGAQAVVFPTVEILAPRDSA
                     PIDRALIHLPDYDWVVFTSANAVEATFDRVGGTPLEGPRIAAVGPATAAEIGRRGARV
                     DFVPSEYLSARLGLELPDVDGRKVLIPQGDQAGTALLEALIRRGARVDVVEAYRTAPP
                     EHPDPGTLAELAVGVDAAIFTSGSAVRHLFALLGDDGAHLALRGAVIACIGPVTAGVA
                     RALGLEVHVEPAEHTVPAVAEALRVRLAA"
     gene            complement(14243..15172)
                     /gene="hemC"
                     /locus_tag="VFU75_04590"
     CDS             complement(14243..15172)
                     /gene="hemC"
                     /locus_tag="VFU75_04590"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: protein motif:HMM:TIGR00212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HEU5466143.1"
                     /translation="MTAGLVTLRVGTRASALARWQTTRVSEALERLTGVPSHPIVIST
                     TGDRALDTPLPALGGKGAFTEELEQALRDGRIDFAVHSLKDLPVVDPAGITVAAILSR
                     DDPRDVVIAREAGSLADLPSGAVVGTSSNRRMAQIAALRPDVTIRPLRGNVDTRVGKA
                     LQGEYDAIVIAAAGVQRLGLTAHITEYLPFHQMLPAPGQGALAVQCRADDDAVRTLLM
                     VLDDPAVRVPTTAERAFLSDLGGGCAAPIAALGESFEGEGRRQLRLTGLVASLDGRRV
                     VRATLEGEWNGGEALAQALAERVTAGGARALLS"
     gene            complement(15169..16500)
                     /gene="hemA"
                     /locus_tag="VFU75_04595"
     CDS             complement(15169..16500)
                     /gene="hemA"
                     /locus_tag="VFU75_04595"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HEU5466144.1"
                     /translation="MSLSCIGINHRTASVSQRERVALGVEAQRRWLNAPETVAHRARA
                     GIAEVTVLSTCNRTELYAAHNAPPSPDLLRDLFFSLADLPVSDVAPHLYEFTGPDALR
                     HLFGVASGLDSMVLGEAEILGQVRTAREVACGEGAAGPVLDAAFRAAAQAGRRARTET
                     GICRLPGSVATEAVRLLASVAGPLEHVSLLIVGTGKMGRVVGEAFRSRGGERISVISR
                     TREHADELAKEWKGIALPWHELPAAVRGADVILSSTGAPHAVLTRELVDQAIGPRGDG
                     RRRVIVDIAVPRDVEPAVGSLPGIVLFDIDDLQLRVGQNLEHRGREVPAVQRIVEEEV
                     QRFDAWLRAWSLRPVLAGMHARGEAIRQREVARMLRRLGPVPPAIREQIESFSRTLVN
                     QLLDAPSRRLREADDPSRAETFGAVTRELFGLDGRDAPDDRGPGDANAEVA"
     gene            complement(16559..17218)
                     /locus_tag="VFU75_04600"
     CDS             complement(16559..17218)
                     /locus_tag="VFU75_04600"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5466145.1"
                     /translation="MNPIRVLVADDHSVVREGLRHVLAGVSELTVVGEAGTGPDAMAL
                     AATARPDVVLLDLTMPGAGGLEVLRHLRTTLPDIRVLILSVHDDPEYVLESVRAGAHG
                     YLRKDSSPAEIRQAIRAVHAGDAYFSPPVARHLTTALRDGTPPAQPEADLGELTSRER
                     EVLIRVARGLTNRETAAELGISVRTVEAHRDSLMRKLRIHTVAGLTRFAISQGLLPAS
                     Q"
     gene            complement(17215..18531)
                     /locus_tag="VFU75_04605"
     CDS             complement(17215..18531)
                     /locus_tag="VFU75_04605"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEU5466146.1"
                     /translation="MDQTPAGGTAGELQAAALLAAITLGLAVLCVFLYRRYRKPFLIW
                     WVAAWGLYLIRGVVIGAFLVTGERPLLYLHQVVTGWTALALLAAALVFTRPRQWRLGY
                     AAAILLPVVWAYVTIYVLNNFLLAAGPTVLLLSGVTLWTGWIFARHWRNVRAPGAGML
                     AATFCLWSLHHLDYPILRARGAWSPWGYYLDILFLLATGAGILVLVEDELRSEHADLT
                     GRLARLSAGLLEQYEAERRRLSLELHDETAQVFTAVKLQLGVLREQSDRALTPALTEI
                     DGLVDTGIQSIRRVTNQLRPSLLDDLGLLPALRSLGDDFVKRSGVRLTIEAPPTVPEV
                     STRAELALFRAMQEALANVVRHAGATHVRVGVIPDGPRLALEVEDDGRGLPPGFDLER
                     GEREGHLGLAGMRERMAALEGEFFIRAAASGGTLVRVAVPCAEEPA"
     gene            18662..19141
                     /locus_tag="VFU75_04610"
     CDS             18662..19141
                     /locus_tag="VFU75_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466147.1"
                     /translation="MNWPHIHLLLNHAPLFGAVFGTLLLALALWRRQELTHTALYVLV
                     FSGFAAIVVFLTGEPAADALANAPEALVDRHEDLAGVATILTGLAAAAALLALLIPPR
                     FGALARWMKGTVLALALGASALLAWTANLGGQINHPEVRRGAVTASPAHPGEEAGQR"
     gene            complement(19138..20700)
                     /locus_tag="VFU75_04615"
     CDS             complement(19138..20700)
                     /locus_tag="VFU75_04615"
                     /inference="COORDINATES: protein motif:HMM:NF013132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEU5466148.1"
                     /translation="MTPETAPAGTRGSVGELLGLDHFRLPATMHRAGTPGEVLMRRAG
                     LPVGVAVFALVLSLPRPEGLPAAGQAAFAAFALALVWWVTEPVPTHVTSLTLMVLLVV
                     TRAWDATHVMGVLGLDVIWLNILAFILSAMLVKTRLAKRLALALILRFGKRASWALGA
                     FVLLQLALAPLIPATAARAALTLPLMTATAAIYGSTAAHPTNFGRNLYLLNLTAISIL
                     SSTVMTGSAANVMAVGFLQTMAGHRVYYTDWLLASGPVAVLVVVAAWALGPRVVFPLG
                     PKDMAPQVAGGLSLVRAERDRMGPLSGSEKKAIAIFLLVIGLWATDRFHKAWFGIELG
                     PSLAAMIGAVVALWPKIGVLEWDETEIPWHLMIFSAGAYAGGLALEESGAAAWGVKGI
                     FGGLPLDRVAFGWTYAAVLAVMLYSHLLSTSKTVRTVIMIPTIILLARGLGWEPASLA
                     LPAAFTIDWVIGLPISGKPNVILFATNQYSARDNLKYGLAVCTAGYVLLLIAGATWFH
                     WLGLTPAFSATP"
     gene            complement(20690..>20932)
                     /locus_tag="VFU75_04620"
     CDS             complement(20690..>20932)
                     /locus_tag="VFU75_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466149.1"
                     /translation="LCRRPLIEKTRYHVPWRGAMWDVDVFDGENAGLAVAEIELESVG
                     APVILPPWVGLEVTDDPRYLNASLIQRPFIRWSHDA"
BASE COUNT         3530 a         7383 c         7039 g         2980 t
ORIGIN      
        1 ggccaacggc cgctgcaact cgctccccgc cccgaggccg agggccagcg gcagcagacc
       61 gaagagggtg cagagcgtcg tcatcagaat gggacgcaat cgcacgcgcg cggcatcccg
      121 gagagcggcc tccagattca gtccgtcccg ccgcatgcgc agcctcgtga agtcgagcag
      181 gatgatgccg tttttcacga tcagccccac cagcaggatg aatcccatca tcgaagacac
      241 gttgagcgcc gtgccggtca ccaggagcag cgcgacgcct ccggtgaatg acaccggagc
      301 cacgagcagc acgatcagcg gctcgacgaa cgactcgaat tgcacgacca tcacccccac
      361 cacggacagc gaggcgagcg ccaggacgat caccagcgcc ccgaaggctt cctgttggtt
      421 ggcgtactgg cccgcgagca cgacccgaac gccggccgga ggaggctcat cggcgagtac
      481 ccgccggaca tcgcggctca cgtcccccag ggagcggccg ctcacgtcgg cgttgatatc
      541 gatcatctgt tgctgattct cgcgcaggag ctcggcacgc gaatccaccg gcaggaaatc
      601 ggccacggca ccgagaggag tcgaccgcgt cgccccgctc gccagtagcg gcacactcgc
      661 cagccgcgcg atgtcgaagc gcaccgcgtc gggcgcccgc acgcgcacgc cgatcggccg
      721 gtcctccgca tggacctcgc cggcgtcggc ccccaggagg gcgccgctga ctgcatcccc
      781 gacctccgca ggtgtcagcc ccatccgcgc ggcctcgccc tcccgcacct tcagcagcaa
      841 ctcggcagtc ggttcgctga ccccgttgta gaggtcctcc aagccttcga ttttttccag
      901 cggactctgg agacggcgcg cgtaggcctc gagcgtgtcg agattctccc cgaatacctt
      961 cacttcgacg ggcttcgcgg cacccgccag gtcgttgatg acatcggaca ggatctgcac
     1021 gaactcgatg tgcaggcgcg ggaccgccgc gccgatcgag tcacgcacgg cgtccatcac
     1081 cgcgaatatg tcccgggggc gctcgccctg cggcttcagg cgcgcgacca gatcccccga
     1141 gttctgcagc gtggcgaaca aacccatctc ggcgccggtg cggcgcgacg ttcccacgat
     1201 ctcgggcgtg cgggccagga tgccttcggc gatccggacc tcacggtcgg tttcggccag
     1261 cgccgttccc ccgggggtga agtagtcgag cacgaacgcc ccttcgtcca tttcgggcag
     1321 aaatcccgtc cccaccagcc ggtacccggc gagacccgtg gcggccaccg cgagcagggt
     1381 gaacacgagg cgccgagggt gatggagggc cgccgccagc gtccggccgt accagtccgt
     1441 gaggcgggtc agccccgcat ggacccggcc caggaaggac cggtcgttct ccggcgccgc
     1501 ttcgtgcgcg gtgacgaact gctcggcgag gagcgggatg atcgtgaacg caagaccgag
     1561 cgagaccagc accgcgcacg tcaacgtcgc cgccagggcg gcgaagaact ggcccaccac
     1621 gccggtaagc aggccgaggg ggaggaacac gaccacggtc gtgatggtcg aggtggtcac
     1681 cggccagagc agctcctgca gcgcttcgcg gatcgcgagc cggcggtcgg acgtcagcgc
     1741 caagtgtcgc gcgatgttct cggtgatcac cacggcgtcg tcgatcacga gtccgatggc
     1801 gatggccatc gcccccagcg tcatcaggtt gaacgtctgt ccgagcgccc gcatgccggc
     1861 aacggtgatg atcagcgtca gagggatggc ggtggcgctg atggcggtga tccggccgtg
     1921 ccggagaaac agcaggagga tgatcaccgc gagtgccgca ccaatgatca tcgcgtcgcg
     1981 caccgaccgg acggcatccc gcaccagcgc cgcctggtcg tagatcgctt tcacctgcac
     2041 acctggaggc agctcgggcg cgagcgcgcg gagcgccgcc gtcgcgctgt ccgcgatgtc
     2101 cagggtgttg ccgccgagct ggcgggtcag gctgatctgc gccgcgggct tcccgtcccc
     2161 ggcgatgatc cgcacgtgat cctcggtgcc cagggagacg gtcgcgacgt cgcggacgcg
     2221 gagcccgttg ggaaggacca ccgcgcctac gtcctccgtc gagcgggctt cctgatccgt
     2281 gacgacgagg tactgcttgt agtctctcga tacccggccc acagcttgca ccgtgatggc
     2341 ctgccggatg tccgcggcca gaccgtcgta ggtcagcccc agggccgcga ggcgcccggg
     2401 atccgccacg acttcgacct cgtgcacgtc ggacccctgc acgatgaccc gccccacgcc
     2461 cggaatccgc gacaggaccg ggcggatctg gtagcgggcg atgtcgtaca gcgtggcggg
     2521 gtcgccgcct tcgagattgt acgaaagaat gggaaacagc gagggcgtca tgagctcgat
     2581 ctggacatcg agccccgcgg gcaaggtggg ttcgacctgg ttgacccgcg cccgcgtcaa
     2641 gctcaaggcc tgcgtcatgt cggtgtgcgg gtcgaaatag accgacgtct ccgtcgcgcc
     2701 gcggatggtc cggctgtcta cgcgcaccac gccggggacg atgctgatcg cctcctccag
     2761 cggacgggtg acactgaaga ccatctgccg ggcccccagc gcggagccct ccactacgac
     2821 ctggatgcgg tggaacgcga gctcaggata gatcgccgac ggcagcccca ccgcgaacca
     2881 ggcccccgcg gccgtgagca gcgccaccgc caggtagatc acccgccgct ggcgcgacag
     2941 gacttcgaag aacttcacgg tttggccggc cgggcgacgg gagccacccg ggcgctgtcc
     3001 gtgatgccgt aggcgccggt ggtcacgacc gtctgtccgg gtgccacgcc cgaccggatc
     3061 tccgccaggg agtcactccg accccccaac accactgggg tacggtgcgc tatccccgtg
     3121 ctatcgacga tgaagacgtc gtacccctcg ccattcggca ccagggcggc ttcgggaatg
     3181 accaccgctc gggggacggt gaggacggca atccgccccc gcacggcttc cccgatccga
     3241 agcgcgcgtg tggcccgcga cagatgtgcc cggacggtga tggtgcggct ggccggatcc
     3301 accgcggccc ccacatcgcg aacccgcccc gccccaagcg tgtcgccccc cacctgatct
     3361 cccgccatga cgagaaccga atcgccagga cggacgcggg ccgcttgatc cactgacaag
     3421 gcgaagagca gatcgagggc atcgggatcc gcgacggcga cgacgggctc cgagggatcg
     3481 gcggggccgc ccaggaccgc cgtcagccgt gtgaccacgc cggcgagggg cgactgcagg
     3541 gccgccagct gggcgttccg gcgcgccgtc accgccgcgg tctgcgccgc ggccaggtcc
     3601 agctcggcct gatcggcgtc cttcctgggc agtatccccg cggtcaccag ccggctcgcg
     3661 cggtcggcgg cgtgctgggc ggccgcgagc gcgacgtcgg cggcgtgcgc cgcggcgtcg
     3721 aaggacgcct gctcgaacac caccagcgtg tcacccacgg cgacgcgctg tcccggcgtc
     3781 acgagaatgc gcgccactcg cgtcggcgcc ggcgcggcca gctccgcata gcggcctggc
     3841 cggggggcga ccgtcccgat ggcggtcaca atttccgtaa agggtccctg tcgcaccgtc
     3901 gccgtggcga cgggggccgc cgccggctga tccgcctcgt gtcctccgcc gtggcaggcc
     3961 gcgatcagga cgagcagtgt cacgaggcgt ttcacggatg ctcccggttc gcgtcgagga
     4021 gtttgaccaa ccctacggcc gtattggcca gcgccagatc gtcgacatag cggcccaatg
     4081 cctcgcgcgc gttgcgctgc gcctcgagta cctcgggcaa cgagacggct ccttcaccgt
     4141 aggcctggag gaacatcgcg gccacggaat ccgcactcgc caccagcccc gcatcccggt
     4201 gggctcgggt cagcgccacc gaacgctccc ccacggcgcg ggctcgctcc gcggccgcgg
     4261 cgcgccgcgt cgcatccaga cggatctgcg ccgcatcgcg ctcggcccgt gcgcgcgtga
     4321 tcgcgccgcc attccagttg aagagcggca aggggagcgc aaacccgaag gtcgggagcg
     4381 cgccgggctc cgacccggtc ggatctcccc gttcgacgcc cacctgaaag ctgggggaga
     4441 ggaggttcac ggatttcgcg agtgtgagcg accgttcagc cgccgtcagc tcagcctgcg
     4501 ccgcgagtac cggcaggggg aacgagggta tctggtcccc ggtggtgtcg ggaaactgga
     4561 gggaatcact caacaccacg gccgagtccc caggaagtcc catgacgagc tgcaggttca
     4621 cgagcgccgc gacggccgcg agcgaatctt cggccgcggt gttcgccagc tcccccgcgt
     4681 tgatctccgc aagccgggta tccatctcgc tcgcgtcccc ggcatcgcgc ctgagacgcg
     4741 ccacccgcaa gagtgagtcg gcgtcgcctg ctgtccggtg ggaaagccgc gcatgcgcct
     4801 gagccgcgag tgcgacggtg tacgccgtat cggcatcgaa gcgcaccgcc gcgacttcaa
     4861 aagtgaaccg cgcacgcgcc gcctccgctc cggcgccggc ggcgccgacg cgcggcgcgc
     4921 gcagccacgg ccagtcgatc ggaatgtcga agatgtagtg gtaccgcggc accgatttcg
     4981 tgtacgtggc cgagaacgac ggattggcga acgcccgggc ggcggcgaga tcgccgtacg
     5041 cagcggccga gtcggcgcgt gcggtagcgg cgcggggtcc gtgggccagc gcgaacgcga
     5101 ccgcatccgc gcgggacacc gcgcgaggcg tctgcgccga aaggccagaa cacggcagaa
     5161 gcaggaagac caggaccggg cgcatagggc ccgcaagcta tccgtttccc cttacgggag
     5221 gcttacacct cggcgggaaa ccgcacgacg acttcggtgc cggagccgtt cgaggagatg
     5281 tcgatcttcg cctgatgcgc gtcagcgatc cagcgcgcga tggcgagtcc cagcccggct
     5341 ccttcgttcc cctccccggc gcgacgggcc tgctcacccc ggtagaagcg ctcgaacacg
     5401 tgcggaagct ggtcagccgg aatcccgatg cccgtatcca cgacccgcac cgtcggcgtt
     5461 cccttctcga cactcgcatc gatccgcacg cgcccacccg ccggcgtgaa cttgatcgca
     5521 ttgtccagga ggatgcgaag cagctgccgg agggggacgg gatccccgcg gacggcgcat
     5581 tcctcgaacg acccgacggc gacggtcaca ccacgccggt cggcgagggg acgcagatcg
     5641 ttcaccgtat cggctgccag atcgtccaga aatacccgct cccgagccaa cgggcgttcg
     5701 cccgcatcgg cccgggccaa cgtgagcaag tcgccgacga tcgccccgat cctccccgct
     5761 tcccgcgcga cggccttcag gagggccgtg tcccggtcgg ggtcacgggc ctgatcgagg
     5821 gcgaggtcgg cgcgggtacg caggatcgtg acgggtgtgc gaagctcgtg ggcggcatcg
     5881 gccatgaagc gccgcatccg atccatcgag cgttcgatgg gcgcggtgga ttgccgcacc
     5941 aggaaccagc cgccggcggc caccagcacc agggcgccga gcgctccggc ggcgagtgtc
     6001 tcgatgagcg aggcgtactg gtcttcgagc tcgacgcgat cggcggtggc gagcgccaca
     6061 taccgggtcc cggtacctcc cgtgaaccgc tcggcgtgca cgcgggtgtc gtgatcaccg
     6121 ggtccgtcga tcgtcaggtt cacacttccc gtttgcgcgg ctcgccgtgc cacgctggcc
     6181 atctcggggg gcaccgtgga cgggatgatc gcgccaccgg ccgagtcgaa gaagtacaag
     6241 gtgcgatcgg ggatgtggag ctcctccacc gcgtcggcga cggggccgcg cgtctgggcc
     6301 cgttccacct cgcgaacacg cgccgcctgc tcgagggagt gggccgccgc ttcgagggac
     6361 aggtccagct ggcgggagat ctggtgtcgg atggcgcgga agagtccgaa tcccagcacc
     6421 aggaggatga gcatgaggac gccggcgtac cacagcgtga gctggagccg caggcgggtg
     6481 agcggcttca tccgaggcgg tatccggcgc cgcgaacggt ggcgatcagc ttgggcgtga
     6541 acggatcgtc gatcttcgcg cggagccggc gaatcaggac gtcgaggacg ttggcgaagg
     6601 gatcgtggtt gtcatcccag acgtgcgccg tgatcgcagc ccggtcgacc accgcgcccg
     6661 catgcagcac gaagagctcg aggagcgcga attccttgac cgtgagcgtg atcgccttgc
     6721 ccgcgcgcga caccgtccgc gcggaaaggt cgacggtcag gtcggcgatc ttgtgaacgg
     6781 ccggggtgac cgacggccgg cgccgggcca gggccttgat ccgggcgacg agctcgcgga
     6841 agtcgaaggg tttggtgagg tagtcgtccg cccccgcttc caaaccgcgg acgcggtcgt
     6901 cgacggcgtc cagggcggtc aacatcagga cgggcgccgc gaccccttca tcccgcagac
     6961 gccggcagag atcgaatccg gtgccgccgg ggagccggac atcgaggatc acgacgtcga
     7021 aatcggtgcc ccggccccgt tcgagtccat cggcgaacgt cgtggcgaga accggatcga
     7081 tccgctgctc ccgcaatccc tcgcggagga gagcggccag ctgggggtcg tcctcgacga
     7141 cgagtacgcg catgcggcga atctaatgtc ggcgggaccg gacgtcgctt cggccgtcag
     7201 gtcggcgtca gatgacccgg agcgcccggc cagcagcctc caccgttcgg tcgatttcgc
     7261 cctggccgtg ggcggccgac agaaaggcgg cctcgaactg ggaaggaacc cagtacacac
     7321 cgtgctgcag gagagatctg aagaacgctc cgaaccgggc cacgtcggcg cgccgagcct
     7381 catcccagct gcgcaccggg tgatcggtga agaacagagt gaacatcgcg cccacccggt
     7441 tgacgacgca tggcaccctg gcggatcgcg ccgcaccgtt caggccctcg acgaggtccg
     7501 cggtggtctt ggcgatggag tcccagacgc cggggcgacc gagctccgtg agcgtcgcga
     7561 gcccagcggc catcgccagc ggattcccgg agagcgtgcc cgcctgatac acgggaccgg
     7621 ctggcgcgac gagctccatg atctcgcggc ggccgccgta agctcccacg ggcaagccgc
     7681 cgccgatgat cttgccgagc gtggtaaggt cgggcgtgac acccgtcatc ccctgcacgc
     7741 ccgacggatg cgcgcgaaat ccggtcatca cctcgtcgaa gatcagcaag gcaccggccc
     7801 cccgcgtgag gtcccgaagc cctccgagga atccaggctc gggcggcacc acacccatat
     7861 tccccgccac cggctcgacg atgaccgcgg caatgttctt gccgtattcg accattatcc
     7921 ggtctaccgc gggcaggtcg ttgtacggca ccaccagcgt ctccgcggcc gtcaggctgg
     7981 gtacgcccgg cgagtcaggg agcgccaagg tggcgactcc ggaccccgcc ttcaccagca
     8041 aggcgtctgc gtgtccgtga taacaccctt caaatttgag aatcttggag cgcccggtga
     8101 acgcgcgcgc cacgcgcagc gcgctcatca ccgcttcggt gccggagttc acgaagcgca
     8161 tcagctcgag cgccggcatc tgagtcctga tcagctcccc gagctcgact tcgcggggtg
     8221 tcggcgcgcc gaaactggtg ccttcgagtg ccgcttcctg caccgcgcgc acgaccatcg
     8281 gatgcgcgtg gccaaggatg agtggtcccc acgacaggac gtagtcgatg tagctgcgtc
     8341 cgtcgacatc ttccaccgtg gcacccgaac cgcgagccag aaacacgggt tgcccaccca
     8401 ccgcgcgaaa cgcccggacg ggcgagttca cgcctcccgg aaacagtttc tgcgcccgtt
     8461 caaagaggag cgccgaacgc gagggagtca ggcgggcacc cgcagggagc ggagcacctg
     8521 gtcggcggcg gcccatccgg aggtgatgca atccgggatg ccgacacccc ggtacgaagc
     8581 tcccgccaca tgcaggcccg gcaagccgtg caaccaggcg gcgatccgct ccatccggtc
     8641 gagatggccg atcacgtact gtgggagccc gcgcgaccag cggaacaccc gcgcgagcac
     8701 cggcttctgc acgactccgc agaccttctc cagctcccgg cgcgcctcgg cgatcagcgc
     8761 gtcatccgct tcgtcgaccc acccttcgtc tcccgcccgt ccgaggaaga cccgcaccaa
     8821 cgcgccaccg gcgggggcac gcccctggta tttgttggac gaccaggtca ccgccaccgc
     8881 cgctccgcct tccgcccggg gacagacgta ccccgatccg ccgaatgccg gtgcgtcggc
     8941 atagcccagc gagaccgtgg cggacgagac gaaaggaatc cgcgacaact cgatggcgag
     9001 ggagggattc aggtcttcca cgagctcggc tgcgacatgg gccggggtgg cgatgatgac
     9061 ctggtcgaag gtgtcggtgg ctccgtcgtc gcaccggatg cggaacccgt cgccctccgt
     9121 cgtgacgctt cgtgcaccga cccccaggcg cagggtctgg gccggaagtc tggccaccgt
     9181 cgcacggacc aaatgctcga cgccgtccgg aaacgacagg aatccgcccc gctctccctg
     9241 cggcacaccg cgcttccgcc aggtcgagcg cagcacgcct cccgcatcgc gctcggcgcg
     9301 cacgagctgc gggaaggtgg ccgacagcga gagctgttcc ccatctccgg cgtagactcc
     9361 cgacagcagc ggctccacga cccactgata ggcttcgaga ccgaaccgcc gggaagcgaa
     9421 ctgggcgatg ctctcatccc cgcccccgcc acgcggcacg aacgcctcga gtcccgcccg
     9481 gatccggccg gtgggggaca ggagacgggt ggtgagcaac gaggcgatgc gcgacgggac
     9541 gagtccggta atgccttcgg gaagcggatg gaggcggccc tgtcgcttga cgaacgtccc
     9601 gcggtgcgcg gggtcggttc catgcaggag accgtcaatc ccgagcgacc gtgccaaggc
     9661 tacagcgccg ggtttggacg agagaacaca gtcggggcca ccctccacca ggaagccccc
     9721 aaccgactgg ctgacgatct tgccgccggg ccgatgatcc cgctcaagca ccgtgcagga
     9781 gacccgcgcc tcgaccagtc gctgagcagc cgcgagtccg gcgatacccc cgccgatcac
     9841 cgccacccgc gtcatgacca cccctcgcgc cgggcggtgt cgagcaccag gtcggccaac
     9901 cccgccatca tgagcgggtc gtcgttgacc atgcccgccc gttcgagatg cactcccagc
     9961 tcccccgcct gacatctcag ctccacatcg aggtcgtaga gcacctccac gtgttcgcac
    10021 gtgaagccga tgggcgcgac gacgacgttg cgctccccgg catcggccag gtccttcagg
    10081 accgtcccca cgtcaggccc gagccaggga tccggcgtca gggccgccga ctgaaacgcg
    10141 aactgatgcc ggagtccgcc gttgccgatc cgtgcgacga tggcgcgcat cgacgcggcc
    10201 agctgatcgg gatacggatc cccggactcg agaatgcgcc gtggaagact gtgggccgag
    10261 aagatgaccg ggatctcgtg gcggcggtcg gccggaaacc gctccagcgc gtcccgcacg
    10321 cgatccgcgg cggcggcgac gtatcccggg agcaaatgcc agtccgcgat gccgacgaac
    10381 gagagcgcct tcagctcctc gtcgaccttg cggaagtaga gctcgatgga catccgggag
    10441 tagtgcggcg ccatcaccag tcccaccacg tgttcgactc cgcgggctcg aagctcctcg
    10501 agtcccgtgg cgatccgcgg cttccagtgc cgcatgccga ccgccaccgg cacgggacgg
    10561 ccccgcgccg ccagagcctc ctccagcctc cctgcctgtg cccgcgtgcg ttcgagaatg
    10621 ggggatctcc cgccgatcgc ggcgtaccgc cggcggactt cttccactac atggcccggg
    10681 gtgggccggt gcccgcgaac gtcgagcaga tagggctcga cgtcactcag cgcatcgggt
    10741 cctccgtacg ccatgacgag gacgccgaac ggctcgctca tgccgtcagg gtgtgcacca
    10801 ggtcgacgac ccggcgcacc gcatcgacgg gcgtcgtcgg caggatgccg tggccaaggt
    10861 tgaagatgtg gcccgggcga ccctcggccc gcgcgatgat gtcgtgaacc cgcgcctcga
    10921 gctcggcggg tggcgcgaac agcgccacgg gatccaggtt cccctgcact ccacggtcga
    10981 atccgatgcg cctccagccg tcgtcgagtc cgatccgcca atccagaccg atgacgtccc
    11041 cccccgcctg cacgagcagg ggcagcagct cacccgtgtt cacaccgaag tggatgaccg
    11101 ggacgccgag gagcccaagc tgggtaaaca gggcgcgggt atgcgggagg acgtagcgcc
    11161 gatagtcatc cgcgttcaga acgccgaccc agctgtcgaa gacctgaatg atgtcggccc
    11221 ccgcccccac ctgcgcggcg aggtactccc ccagcacccg caccagtttt gtgagcaggg
    11281 cgttccaggc gtcgggatgc tcgtacatca accgcttggt ctcgacgaag tgacgggagg
    11341 accccccttc gatcgcatac gaagcgacgg tgaacggcgc gccggcgaag ccgatgaccg
    11401 gaatggtcgg cggcaaagcc ccgcgcacca gcctcaccgt ctcggccacc ggggcgaggg
    11461 cattggccac gttggtgtcc accaggcggg cgacatcggc cggaccgcgg atgcggttgc
    11521 gcaagacggg cccttcgcct tccacaaagg cgagatcgag tcccatcggg accagcggca
    11581 ggaggatatc ggcgaagacg atcgccgcgt ccactcccaa ccgccacacc ggctgcagcg
    11641 tgatctcggt ggccagctgc gggttggcgc agatctcgag caacgaatgc ttttcacgaa
    11701 tggcgcggta ctcgggcaga taccggccgg cttgccgcat gaaccagacc ggcgtcgggc
    11761 ggccgggttc gcgccgcgcc gcggcgagca gcgtcgaggt gctgggagcc gtcacagcca
    11821 ttgggaggcg gtgccccagt cggcctgatg cacggcggca tgcagcggag tgtcccgctc
    11881 ggtgaagggc ctgccctgcg tactgcggag ctccgtcacc agattcgaga agcgcgtcag
    11941 atcggccgtt tcgtacacca ccacgaactc ctgatcctga agcccggtgg agtagaggag
    12001 caactgggtg atgtcttcgt actgcttccc gagcttgatg tgctccagca tcatgtcgcg
    12061 ccgctcctcc cggctcttct gataccagtc ggaggtcttc gtgaacgggt acatcacgag
    12121 gtagggtgac cgctctgcgg tgaacggatc gatctcctgg cggctcttgg tcttggtgta
    12181 ctgcgacggc tgggtgaagc cccagagcgc ggtggtcaga gtggcgaact cacgaatcgg
    12241 agccaacgcc cgccccagat gcgggaagta acgagcgggg accgccggat ctccgtcatc
    12301 cacggcggtc cagacgagca gatcggtgcc cgcctcgacg ccgaacgtct gatatagatg
    12361 aagggcgtcc acgcctcccc ggagggcttc cagggcggca cagaccgcct gacggcgggc
    12421 cttcggctcc tgcctccagt actgcgcgtc gaaccgcagc agggagaaat ggttgaaggt
    12481 gcggtgcgtg tcttcggcca tgggctactc cgtcagccat cgcgcggcgt cgcgcgcatg
    12541 ataggtgatg atcagatcag caccggcgcg gcgcatcgcc gtcaagtgct ccagcaccgc
    12601 ccggcgctcg tcgagccacc cgcgctccgc cgcggctttc accatcgcgt actcaccgct
    12661 gacgttgtaa gcgacgagcg ggtgatcggg aaaccgcatc cgcgtctcgc ggaccacatc
    12721 caggtacgca agggccggct tcaccatcag gaggtcggcc ccttcgtcca cgtcgaccgc
    12781 ggactcccgg agcgcttcgc ggccattggc gatgtcgagc tggtgtgact gccggtcccc
    12841 ctgtttcggc gctcctccgc ccgcttcgcg aaaggggccg tagaaagcgg acgcgaactt
    12901 cgcggaatac gagagcaccg gaatgtggct gaacgagttc tgatcgagtg ccgcgcgaat
    12961 gccggagacc atcccgtcga gcatggcgga gggtgccacg acgtccgcgc ccgcccgcgc
    13021 gtgagagacc gcgacgcgct tgaggatctc cacgctctcg tcgttcaaga catagccctc
    13081 gggaagctcg ggatgcaccc gccccttccc ggcgccattg agtactccgc agtggccgtg
    13141 ctccgtgtat tcgcagaggc acacgtcggt gatcaccggc aggtcgggga ccgccgcctt
    13201 gatcgcacgc accgcgtcct ggatcacgcc gtggtcgtcg aagctcccgg tgccggtcgc
    13261 atccttggtg cgcgggatgc cgaacagcag aatcgcgccg agccccgcgc ggtgcacggc
    13321 ctcggcttga gcggctatct gggcgaccgg ccattgcgac acaccgggca tcgacgcgat
    13381 ggcgcgcggc tcgcccgacg ccgcggtgac gaacagcgga tagatgagct gcgcgggatc
    13441 gagtcgcgtc tcccgcacca ggttccggag ccccggtgac gcacggagcc gacgcaaccg
    13501 gatcatgcgg cgagacgcac gcgcagggct tccgcgacgg cggggaccgt gtgctcggcg
    13561 ggttcgacgt gcacctcgag ccccagcgcg cgcgccaccc ccgcggtgac cgggccgata
    13621 caggcgatca ccgctcccct cagggccagg tgcgcgccgt catcccccag gagcgcgaac
    13681 aggtgccgca cggcggatcc actggtgaaa atcgcggcat cgacacccac ggcgagttcg
    13741 gccagagtcc cgggatccgg atgctcgggt ggtgcggtcc ggtaggcttc gaccacatcg
    13801 acacgcgcgc cgcgccggat cagtgcttcg aggagagccg tccccgcctg atcgccctgt
    13861 ggaatgagca ccttccgtcc gtcgacgtcc ggcagctcca ggcccagccg cgcgctcaga
    13921 tattccgaag gcacgaagtc cacccgcgcg ccgcgacgtc cgatctcggc cgccgtggcc
    13981 ggaccgaccg ccgcaatcct gggaccttcc aggggcgtac cgccgacccg gtcgaacgtg
    14041 gcctcgacgg cgttggcact ggtaaagacc acccagtcgt agtccggcaa gtggatcagg
    14101 gcccggtcga tgggtgcgga gtcccgcggt gccagaatct ccaccgtcgg gaagaccacg
    14161 gcttgtgctc cccacgattc gagcgcggtg acgaggtccc gcgactgggc gcgcggccgg
    14221 gtgacgagca ctctgggacc ggtcatgaga gcagcgccct ggcgccgccg gcggtcactc
    14281 gttcggcgag cgcctgcgcc agggcctcac cgccgttcca ttcgccctcg agtgtcgctc
    14341 gcaccacccg gcgcccgtcc agggaggcca ccaggcccgt gaggcggagc tgtcgacggc
    14401 cttcgccctc gaacgactct cccagcgcgg cgatgggtgc cgcacatccg ccacccaggt
    14461 cggacagaaa cgcgcgttcg gcggtggtcg gaactctgac cgcggggtcg tcgagtacca
    14521 tcaacagggt ccgcaccgcg tcgtcgtcgg cccggcactg caccgccagc gctccctgtc
    14581 ccggtgcggg cagcatctga tggaagggga ggtattcggt gatgtgggca gtgagtccga
    14641 ggcgctggac ccccgccgcg gcgatgacga tcgcgtcata ctctccctgg agtgctttcc
    14701 cgaccctggt atcgacattc ccgcgcaggg gccggatcgt cacgtcgggc cggagagcgg
    14761 cgatctgcgc catgcgccgg ttgctggacg tgccgaccac cgcgccggag ggaaggtccg
    14821 cgagggatcc agcctcccgc gcgatcacga cgtcgcgtgg atcgtcacgg gagaggatgg
    14881 cggccaccgt gatccccgcc gggtcgacta ccgggagatc cttgagggag tgcacggcga
    14941 aatcgatccg cccgtcgcgc aaggcttgct ccagctcctc cgtgaacgct cccttcccgc
    15001 cgagcgcggg gagcggggtg tcgagcgcgc gatcgccggt cgtggaaatc acgatcgggt
    15061 gcgacggaac gcccgtgagc cgctcgaggg cctcgctcac gcgggtcgtc tgccaccgtg
    15121 ccagtgccga cgcccgcgtc cccacccgaa gcgtgaccag gcccgcggtc atgcgacctc
    15181 cgcattcgcg tcacccggtc cgcggtcatc cggggcgtcc cggccgtcga ggccgaacag
    15241 ctcgcgggtg acggcaccga aggtttcggc gcgggaggga tcgtccgcct cccggagacg
    15301 ccggctgggc gcatcgagga gctgattgac gagcgtccgc gagaacgact cgatctgctc
    15361 gcgaattgcg gggggcaccg gacccagccg gcggagcatg cgtgccactt cccgctgccg
    15421 gatggcctcg ccccgggcgt gcatgcccgc gagcacgggc cgcagactcc aggcccgaag
    15481 ccacgcgtcg aagcgctgca cctcctcttc gacgatccgt tgcacggcgg gcacctcacg
    15541 gccgcggtgc tccaggttct gtccgacccg gagctgaagg tcgtcgatgt cgaagaggac
    15601 gatccccggt aacgatccca ccgcgggttc cacgtcacgg gggaccgcga tatccacgat
    15661 cacccgtcgc cggccgtcgc cccggggtcc gatggcctgg tcgacgagct cccgggtcag
    15721 cacggcatgt ggcgcgccgg tcgaggagag aatcacatcc gcgccgcgta cggcggcagg
    15781 cagttcgtgc cacggcaacg cgatgccctt ccattccttg gccagctcgt cggcgtgctc
    15841 acgcgtgcgg ctgatgaccg agatccgttc cccgccccgg ctccggaacg cctcgcccac
    15901 cacccgcccc atcttcccgg tgccgacgat caacagggac acgtgctcga gtgggccggc
    15961 gacggacgcc aggagccgga cagcctcggt cgccacactt ccggggagcc ggcagatccc
    16021 ggtttcggtt cgcgcgcgcc gcccggcctg cgccgcggcg cggaaggctg cgtcgagaac
    16081 ggggccggca gcgccctcac cgcaagcgac ttcgcgggcg gtccgcacct gtcccaggat
    16141 ctcagcttcg ccgagtacca tggaatcgag tcccgaggca acgccgaaca gatgccgcag
    16201 cgcgtcagga ccggtgaatt cgtagagatg cggcgcaacg tcgctcaccg gcaggtcggc
    16261 gagtgagaag aagagatcgc ggaggagatc gggagatgga ggcgcgttgt gcgccgcgta
    16321 gagctcggtg cgattgcagg tagacagtac ggtgacttcg gcgatgcctg cccgcgcccg
    16381 atgagcgacc gtctcgggag cattcaacca gcgccgctgg gcctcgacgc ccagagcgac
    16441 tcgctcacgc tgggacaccg atgccgtgcg gtgattgatc ccgatgcagg acaacgacat
    16501 ggcgcgattc tatgccaagc gcgcctcaca gaccattcga aatcctacca ccgtactgct
    16561 actgagaagc gggcaagagg ccctgcgaga tggcgaagcg cgtcaatccc gcgaccgtgt
    16621 gaatccgcag cttgcgcatg agggagtcgc ggtgtgcttc cacggtccgg acgctgatgc
    16681 cgagctccgc cgccgtttca cgattggtca gcccccgcgc gacccggatc aggacctcac
    16741 gctcccggga tgtgagctcg ccgagatcgg cctcgggttg cgcgggcggc gtcccgtccc
    16801 gcagggccgt agtgagatga cgagcgaccg gtggagagaa gtaggcgtcg cccgcatgta
    16861 cggcccggat cgcctgacgg atctccgccg ggctggagtc cttccgcaag tacccgtgtg
    16921 cccctgctcg aacgctctcg agcacgtact cggggtcgtc gtgcacggac aggatcagca
    16981 cccggatgtc gggcaaggtg gttcgcagat gccggaggac ctcgagcccg ccggctcccg
    17041 gcatggtcaa gtcgagcagg acgacgtcgg gccgcgcggt cgccgcgagg gccatcgcgt
    17101 caggccctgt cccggcttcc cccaccaccg tcagctcgga cactccggcg aggacgtggc
    17161 gcagaccctc ccgcaccacc gagtggtcgt cggcgaccag cacgcgaatg ggattcacgc
    17221 cggctcctcc gcgcagggaa ccgccacccg taccagggtc cctcccgacg cggcggcccg
    17281 tatgaagaac tccccttcga gcgccgccat gcgctcgcgc atccccgcga gccccagatg
    17341 cccctcgcgc tcaccccgct caaggtcgaa gccggggggg aggccacgtc cgtcgtcctc
    17401 cacctccaag gcgaggcgcg ggccgtccgg aatgactccg acccggacat gggtggcccc
    17461 ggcgtgacgg accacattcg cgagcgcctc ttgcatggca cggaacagcg cgagctccgc
    17521 gcgtgtcgaa acctccggga ccgtcggtgg cgcctcaatg gtcagtcgga cacccgagcg
    17581 tttgacgaaa tcgtccccca gggaacgcaa cgcgggcaag agacccaggt cgtccaggag
    17641 cgaaggccgc agctggttgg tgacgcgccg gatgctctgg attcccgtgt cgaccaggcc
    17701 gtcgatctcc gtgagcgccg gtgtcagtgc ccggtcggac tgctcgcgca gaacaccgag
    17761 ctggagcttc acggccgtga acacctgcgc ggtctcgtcg tgcagctcga gagacaaccg
    17821 gcgccgctcg gcttcatact gttcgagcag accggcggac aggcgggcga gacgtccggt
    17881 caggtccgcg tgctccgagc gcaattcatc ttcgaccaga accagaattc ccgcccccgt
    17941 ggcgagcaag aacagaatgt cgaggtagta tccccacgga ctccaggctc cgcgcgcgcg
    18001 aaggatcggg tagtcgaggt ggtgcaggct ccagaggcag aaggtcgcgg ccagcatgcc
    18061 ggcaccgggc gcgcggacgt tgcgccagtg acgcgcaaag atccagcccg tccagagtgt
    18121 gacgccgctc aggagcaaga ccgtggggcc cgcggccagg aggaaattgt tcaatacgta
    18181 aatcgtcacg tatgcccaga ccaccggaag caggatcgcc gccgcataac cgaggcgcca
    18241 ttggcgcgga cgcgtgaata cgagggccgc cgcgagcagt gccagggccg tccagcccgt
    18301 caccacttgg tggagataga gcagcgggcg ttcaccggtg acgagaaacg ccccgatgac
    18361 gacgccgcga atcagataca ggccccacgc cgccacccac cagatgagga agggcttccg
    18421 gtaccggcgg tagaggaaga cgcacagcac cgcgagcccc agggtgatcg ccgcgaggag
    18481 cgccgccgcc tgcagttctc cagccgttcc gcccgctggg gtctgatcca taggggtgca
    18541 tactagccgg agtccaccgt aaagaaaggc ttcttacgct gcctttcaca gccgattcat
    18601 ggtcggccaa ttacttagag gagcccacgt gcgtctcggg atcccttgac cggcggtact
    18661 catgaactgg ccccacattc acctgctgct caatcacgcc ccattgttcg gcgccgtctt
    18721 cggcaccctc ttgctcgcct tggccctctg gcgccggcag gagctgacgc acacggccct
    18781 ctacgttctc gtcttctcag gattcgcggc gatcgtcgtg ttcctgacgg gcgaacccgc
    18841 cgccgacgcc ctcgcgaatg cacccgaagc gctggtcgac cgccacgaag acctggcggg
    18901 tgtcgccacc attctcaccg gcctcgccgc ggccgctgcc ctgttggcgc tgctgatccc
    18961 gccccgtttc ggagcgctgg cccgctggat gaaggggacg gtcctggcct tggccctcgg
    19021 cgcctcggcg ttgctcgcgt ggacggccaa cctgggcggg caaatcaatc accccgaggt
    19081 tcggcgcggt gccgtcacgg catctcccgc ccatccgggg gaagaagcag gacagcgtta
    19141 gggcgtcgcg gagaacgccg gcgtcaaccc gagccagtgg aaccaggtag cgccggcgat
    19201 gaggaggagg acgtaccctg cggtgcagac cgcgagcccg tacttgaggt tgtcccgcgc
    19261 ggaatactgg ttcgtcgcaa agaggatcac gttgggcttg ccgctgatgg gcaggccgat
    19321 cacccagtcg atcgtgaagg ccgccgggag cgccagactc gccggctccc atcccagacc
    19381 ccgtgccagc aggatgatgg tggggatcat gatgacggtc cgaaccgtct tcgacgtcga
    19441 caacagatgg ctgtacagca tcaccgccag caccgccgcg taggtccagc caaaagccac
    19501 ccggtcgagg ggcagcccgc caaagatgcc cttcacgccc cacgcggccg cgccactctc
    19561 ctccagcgcg agtccccctg cgtatgcccc ggcgctgaag atcatgaggt gccacgggat
    19621 ctcggtctcg tcccactcga gcactccgat cttcggccac aatgccacta ccgccccgat
    19681 catggcggcc agtgacggcc ccagctcgat cccgaaccag gccttgtgaa agcggtccgt
    19741 ggcccagagc ccaatgacca gcaggaagat ggcgatggcc ttcttctccg acccactcag
    19801 cggccccatc cggtcccgtt cggcccgtac cagactcagg ccgcccgcca cctgcggagc
    19861 catgtccttg ggacccagag ggaacaccac ccgaggcccc agcgcccacg ccgccactac
    19921 caccaacacc gcgaccggcc cgctggccag cagccagtcc gtgtagtaga cccggtgacc
    19981 ggccattgtc tgcaggaacc cgaccgccat cacattcgcg gcgctgccgg tcatgaccgt
    20041 cgaggagaga atgctgatgg cggtgagatt cagcagatac aggttccgcc cgaagttggt
    20101 gggatgggcg gccgtggagc catagatggc agcggtcgcc gtcatcaacg gcagggtcag
    20161 agcggcccgc gcggccgtcg ccggaatcag cggggccagg gccagctgca gcagcacgaa
    20221 cgcccccagg gcccagctcg cacgcttgcc gaatcgcagg atcagcgcca gcgccagccg
    20281 cttcgcgagc cgggtcttga ccagcatcgc actcaggatg aaggccagga tgttgagcca
    20341 gatcacgtcc agcccaagca cccccatgac gtgggtcgcg tcccatgccc gtgtgacgac
    20401 cagcagcacc atcagcgtca gggacgtgac gtgggtggga acgggctccg tcacccacca
    20461 gacgagggcc aaggcgaagg cggcaaacgc cgcctgaccc gcggccggca ggccctccgg
    20521 gcgcggcaag gacaacacca aggcgaagac ggcgaccccc accggcaggc cggcgcggcg
    20581 catgagcact tcgccgggag tgcccgctcg atgcatcgtg gccggcagac ggaaatgatc
    20641 cagacccagc agctcgccga cgcttcctcg cgtgcccgcc ggcgcggtct caggcgtcat
    20701 gggaccatcg gatgaacggc cgctggatga gactggcgtt cagatatcga ggatcatccg
    20761 taacctcgag gcccacccag ggcgggagga tcaccggggc gccgacagat tcgagctcga
    20821 tttcggccac ggccagcccg gcgttctcac cgtcgaagac gtccacgtcc cacatggccc
    20881 cgcgccaggg aacgtggtag cgggtcttct cgatcagtgg gcgccggcag ag
//
LOCUS       DASUBP010000123         9607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_834519, whole genome shotgun sequence.
ACCESSION   DASUBP010000123 DASUBP010000000
VERSION     DASUBP010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9607
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_834519"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1540)
                     /locus_tag="VFU75_04625"
     CDS             complement(<1..1540)
                     /locus_tag="VFU75_04625"
                     /inference="COORDINATES: protein motif:HMM:NF013557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466150.1"
                     /translation="MTGRPTPSVPHLSQVLPTARAILWNPILQGTVRRTPGGAYPIFI
                     DQKALTALTDHFASIPNDQAVFGFLAGDLFECPTSKVRYAVVDSTIRLNQPIYGDKTA
                     VVVQRLWDRIQDELKKIEGQLIGWYHSHPGLSLSLAPEDVKAQETYFTSPWHTALVLG
                     AQGSDNVAGFFRRSGAPGWTTTPLPFYELLDEATVSTRGKRSILPWKNFGTDEATASR
                     AAAGAPAKATPARVGPPPKPSTPAPPPTKLPSPAPAPAAPKAAAPPAPPPPAPKPPPP
                     APKPPPPPPPPPPPPPVEVPLAPPPIAPPSRKAPDAMEVEASIAPAPPAAAPPPPAPP
                     LPPPPAPRRSGGQAMPHLAPVRQSDRTSTPVRPSSRRPTVGKLYAEEPKSWRGRIILG
                     VVVLALLGGLGYWYQVLRPTPGWEYPWSPLVRRFASAKAPAPAPAPKPTTVDSATVRF
                     DAASDSLAAAITRFGDRALRFGGLPSDCAPLDSGLVALEDAWTTYNQRQQNVTLDSQR
                     ATRDQ"
     gene            complement(1537..2562)
                     /locus_tag="VFU75_04630"
     CDS             complement(1537..2562)
                     /locus_tag="VFU75_04630"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntG family PLP-dependent aldolase"
                     /protein_id="HEU5466151.1"
                     /translation="MSAIDLRSDTVTKPSAAMRRAMADAEVGDDVLDGDPTTRRLEQR
                     VAELLGKGDALFFPTGTQANQTGVAMCTTPGTELVLEANAHLVHYEIAGVALLAGVQI
                     RPVTTPDGLLSQDLVKAALRPASPHVPRVSAIAIENTHNAAGGRVLPVDRLRAITALA
                     AERNLPVHLDGARLWNAAAALNQKLSALTAGVATVMVSLSKGLGCPVGSCLAFDAGRR
                     AEAWEIRKRLGGGMRQSGILAAAGLYALDVNLPRIAEDHANAKTFAAIVAENKALRPV
                     PPDSNIVMVDLTREGDAADRAVTGLAAAGVLCTAFGPRRLRFITHLDVSRADVERAAG
                     IVATVLA"
     gene            complement(2559..3449)
                     /locus_tag="VFU75_04635"
     CDS             complement(2559..3449)
                     /locus_tag="VFU75_04635"
                     /inference="COORDINATES: protein motif:HMM:NF015587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEU5466152.1"
                     /translation="MKVEGGWHQSFVGMVRRTFQAAFEDNVPFLASGLSFDLLLTAIP
                     FAALALAVVGYLVQHQITAHQISVHQLLQRFLPGGPTGRGVDIFDQVERALGGIVAQR
                     GRLTLYGLPLFIWFAARFFSGLRIALNEVFDTEEVRPWPVAKGTDLAIMLTTAALVVG
                     NALLPAYEVSSAGTMTNSFWLLWSWRFGLEVTAFGFSTVLFFLIFKVLPSRRIQWRTA
                     LVAAGFCSLAFEVAKRAYALYLARFVTLDRVATDANIIAFFLFLLWIYYTAFVFLLGG
                     EVAETYDLVRMRRAQRVRLV"
     gene            complement(3446..3760)
                     /locus_tag="VFU75_04640"
     CDS             complement(3446..3760)
                     /locus_tag="VFU75_04640"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEU5466153.1"
                     /translation="MSDDEGTPDRSAVGPFLVGLAIGAALGALFAPESGSKIRARVGK
                     RAGQLREDLGDIVDELRTVMATEEPDASPREQLRRRLHAARAARRTLPGAGAGTADTP
                     LE"
     gene            complement(3757..5133)
                     /locus_tag="VFU75_04645"
     CDS             complement(3757..5133)
                     /locus_tag="VFU75_04645"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HEU5466154.1"
                     /translation="MAEEPQDILRRFASPSVRRSDAMDQLVRDAVFGDQGTRDRARWL
                     IWEIGQQAGARPASIHELYLARGRGEVSAFTTPAINVRMMGYDTARAIFRAAVRMDVG
                     AVICEIARSEIGYTEQRPAEYVAVITAAALREGFDHPIFIQGDHVQVNAKKYATDPAA
                     ELSAIKALIDEEIHAGFYNIDVDTSTLVDLSQKTLDEQQRVNFERAAELTQYIRQREP
                     KGVTISVGAEIGEVGGKNSDVHELKAFMGGYERTRHHLGVKHGISKISVQTGTSHGGV
                     VLPDGTIAKVQLDLAALKELSQEARSIYGLGGAVQHGASTLPPDAFSNFPLCEAVEIH
                     LATNFQTMVFDHPKLPQALRAEVNAWVKAECKEEWKKGDTEEQFIYKSRKKAIGPFKK
                     KFWDLPADIRQAIGADLEKTFAFLFEQLRVGNTRAATDRFVKPPLDSHAAPRMAAVAA
                     PDDLEAGE"
     gene            complement(5159..5941)
                     /locus_tag="VFU75_04650"
     CDS             complement(5159..5941)
                     /locus_tag="VFU75_04650"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5466155.1"
                     /translation="MRLTLLGTGTSFGVPQIGCHCRTCTSTDPKDRRGRTAALIEDGD
                     RRILIDTPPEIRLQLVAAGVDRLDAVLFTHAHADHVHGIDDLRAFTVKQRAPLEAYGS
                     RETLAELGAKFPYIFDLESKAPAGTSKPELVARPLVPGVSVRIAGLDVEPLALPHGRQ
                     EVLAYRIGRLAYVTDVKDVPEAARTRLGGLDVLVLSALLSRPHPLHLSVPEAVAVAQA
                     IGARRTFFTHLTHETPHADLAASLPAGIAPAHDGLVIDVGES"
     gene            complement(5942..7339)
                     /gene="pyk"
                     /locus_tag="VFU75_04655"
     CDS             complement(5942..7339)
                     /gene="pyk"
                     /locus_tag="VFU75_04655"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEU5466156.1"
                     /translation="MIRRTKIVATLGPATQSDELIGQLLDAGVNVVRINASHGTPEVR
                     GQWMKAVRRIADQRGLAVAILVDLQGPRIRVGDLKAPVELVPGAEVVFAPEDVAKGAE
                     IPTTYDELAADARVGARILLNDGLLSVEVTKVVAPRVHARVVDGGTLTSHKGMNLPGL
                     QVSAPALTDKDREDVAHAVQHGADFLALSFVRRADDLAELRLLVPSEMKLVAKIEKAT
                     ALDDLPRILSACDAVMVARGDLGVELPFEEVPLVQKRLIRDANAVGKPVITATQMLES
                     MVHAPRPTRAEASDVANAILDGTDAVMLSAETAAGEYPVESVRAMDRIIREMERHLPP
                     RQEQRLSSRGNVTVEDAIALGTSAVARLLRTPLIVTLTSGGFTARKVAAMRPAPPILA
                     VTTLKHTFRQLALVWGITPVLVDRVPGYDAMLAVVRDLILKREYARPGDRIVMTAGVP
                     WEVSGSTNLIKVEEV"
     gene            complement(7405..8943)
                     /locus_tag="VFU75_04660"
     CDS             complement(7405..8943)
                     /locus_tag="VFU75_04660"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU5466157.1"
                     /translation="MVVSAGGTGDAVWWRGGKIEGVGTFADVRSAATVAGGPPPTLIE
                     LPDCLVTPGFVDGHTHFAEWAIGRQRVSLNGCRTRAEAVARAAKGVLTDGWIQGQGFD
                     PNGWEAPPTREALDQVLPNTPAAFDSHDVHSLWVNSTALARCGIDRSTRDPEGGRIVR
                     DAAGEPTGLLLEHAVRLVHRHLPQVGRERVVEAVRGAQGAAHQLGVTGIQNVEGPDAL
                     TAFQRLEEEGALRLRVLAHLPADGLDAALRVGLRSGWGSEWLVTGGVKLFLDGALGSQ
                     TALLLDPYEGSADRGIALMSEADAREALTRAARGGIAATVHAIGDAAVRRALDIIETL
                     PPVAIAHRIEHFQMVHPADLQRAARAGIVASMQPAHLLTDIPLVDKHWGDRGAGVYAF
                     RSLRSAGTALVFGSDVPVETIDPRDGVYAALDRVSRDGLPAGGWRATERLSFDEVLHA
                     YTAAPWVAAGRGGAGGTLAPGEVADLVAWSPPALAGVAGDGAAFRSAQAVLTVAGGET
                     VWRA"
     gene            complement(9184..>9607)
                     /locus_tag="VFU75_04665"
     CDS             complement(9184..>9607)
                     /locus_tag="VFU75_04665"
                     /inference="COORDINATES: protein motif:HMM:NF013231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5466158.1"
                     /translation="AVTAAQSAVERSGDRAFRTYGLTHRAHTALYAVDQGGPDGIRPR
                     EVAEALGISRAAATTFIQRLEAKGLVETTPDQDDDRGVRVTLTRHGLEVLLRVGQLER
                     RLSARTIEGMPATAIQRTIHVLQEYRRRLEGRQIEVLR"
BASE COUNT         1497 a         3364 c         3297 g         1449 t
ORIGIN      
        1 gctggtctcg ggtggcgcgc tgggagtcga gggtcacgtt ctgctgccgc tggttgtacg
       61 tcgtccacgc gtcctccagg gccaccaggc cggagtcgag gggcgcgcaa tcgctgggca
      121 agcccccgaa ccgcaacgcg cggtcaccga accgcgtgat cgccgcggcg agggagtcgc
      181 tggcggcgtc aaaccggacg gttgccgaat cgaccgtcgt gggcttcggt gccggcgcgg
      241 gtgcgggcgc cttggcggac gcgaagcgcc gcaccaacgg actccaggga tactcccacc
      301 ccggcgtcgg gcgcagcact tgataccagt accccaaccc gccgagcagt gcgaggacga
      361 cgaccccgag gatgatccgc ccccgccagg acttcggctc ctccgcgtag agcttcccca
      421 ccgtgggccg ccgagagctc ggccgcacgg gcgtcgaagt gcggtcggac tgacgcaccg
      481 gcgcgagatg gggcatcgct tgtccacctg accgccgagg ggccggcgga ggaggcagag
      541 gaggcgctgg aggcggtggc gccgcggctg ggggtgccgg cgcgatcgac gcctcgacct
      601 ccatcgcgtc cggagccttt cggctcggtg gcgcaatcgg gggcggcgcc aacggcacct
      661 ccaccggtgg cggaggaggc ggaggtggcg gcggtggcgg cggcttcgga gctggcggcg
      721 gcggtttggg tgccggtggt ggcggcgccg gaggagccgc ggctttgggt gcggcgggag
      781 caggcgccgg tgacggcagt ttggtcggcg gaggcgccgg cgtcgacggt ttggggggcg
      841 gccccacccg cgccggcgtc gccttcgcgg gcgcgccggc agccgcgcgg ctggccgtcg
      901 cctcgtcggt gccgaagttc ttccacggga ggatggagcg tttcccgcgc gtcgaaaccg
      961 tcgcctcgtc cagcagctcg tagaacggca acggcgtggt cgtccacccc ggcgcaccgg
     1021 aacggcggaa gaatccagcg acgttatcgc tcccctgcgc gccgaggacg agtgcggtgt
     1081 gccagggcga cgtgaagtag gtctcctgcg ccttgacgtc ttccggcgcg agggacaagg
     1141 acaacccggg atgactgtga taccagccga tgagctggcc ttcgatcttc ttcagttcgt
     1201 cctgaatgcg gtcccacagc cgctgcacta cgaccgcggt cttgtcaccg tagatcggct
     1261 gattcagccg gatggtggaa tcgacgaccg cgtagcgcac cttgctggtg ggacactcga
     1321 acaggtcgcc cgcgagaaat ccgaacaccg cctggtcgtt gggaatggag gcgaagtgat
     1381 cggtcagcgc ggtcagtgct ttctgatcga tgaagatcgg atacgcgcct cccggcgtcc
     1441 gccgcaccgt cccctggaga atcggattcc agagaatggc acgcgccgtc ggcagaacct
     1501 gcgacagatg cggcacactc ggggtcgggc ggcccgtcat gcgagcaccg tggcgacgat
     1561 gcctgccgcc cgctcgacgt cggcgcgcga gacgtcgagg tgcgtgatga agcggagacg
     1621 gcggggcccg aaggcggtgc acagcacgcc cgccgccgcc aggcccgtca cggcccgatc
     1681 ggccgcgtcc ccttcgcgcg tgagatccac catcacgatg ttcgagtcgg gggggaccgg
     1741 gcgcagagcc ttgttctccg ccacgatggc ggcgaacgtc ttcgcattcg cgtggtcttc
     1801 cgcgatgcgc gggaggttga cgtcgagggc gtacaacccg gccgccgcca ggatgccgga
     1861 ctgccgcatg ccacccccca gccgcttccg gatctcccac gcttccgccc ggcgccccgc
     1921 gtcgaaggcg aggcacgatc ccaccggaca gcccagtccc ttggagagac tcaccatcac
     1981 ggtcgcgacg ccggccgtga gggccgacag tttctgattc agcgccgcgg ccgcgttcca
     2041 gagccgggcc ccgtcgagat gcaccggcag gttccgctcg gcggccagcg ccgtgatcgc
     2101 ccgcaggcgg tcgacgggca acacgcgccc ccccgccgcg ttgtgcgtgt tctcgatcgc
     2161 gatcgcgctg acccgcggaa cgtgcggcga cgccggccgc agggccgctt tcacgagatc
     2221 ctgggagagc aggccgtcgg gcgtcgtgac cggacggatc tggacgcccg cgaggagcgc
     2281 gacaccggcg atttcgtagt gcaccagatg ggcgttggcc tcgagtacga gctccgtgcc
     2341 cggcgtggtg cacatcgcca cgcccgtctg attggcctgc gtgccggtcg ggaagaagag
     2401 ggcgtccccc ttccccagca gctcagccac ccgttgctcc agccgccggg tggtcggatc
     2461 gccatcgagg acgtcgtcgc ccacctcggc gtcggccatc gcccgccgca tcgccgcgga
     2521 gggtttggtg acggtgtcgg accggagatc gatcgcgctc atacgaggcg cacccgctgg
     2581 gcccgtcgca tgcgcaccag gtcgtaggtt tcggccactt cgcctcccag caggaagacg
     2641 aacgccgtgt agtagatcca caggagaaag aggaagaacg cgatgatgtt cgcgtccgtc
     2701 gcgacgcgat ccagagtcac gaagcgcgcc agatagagcg cgtaagcacg cttcgcgacc
     2761 tcgaacgcga gggagcagaa cccggcggcg accagggcgg tgcgccactg aattcgccgg
     2821 ctcggcaaca ctttgaaaat gaggaagaag aggacggtgc tgaatccgaa ggcggtaact
     2881 tcgagcccga agcgccagga ccagagcagc cagaaggaat tggtcatggt gccggcgctc
     2941 gagacctcgt aggcgggcag caaggcgttg cctaccacca gggccgcggt cgtcagcatg
     3001 atcgccagat cggtgccttt cgcgaccggc cagggacgaa cctcctcggt gtcgaacacc
     3061 tcgttcagcg cgatccgcag gcccgagaag aaccgggccg cgaaccagat gaagagcggc
     3121 aatccgtaca gcgtgaggcg cccgcgttgc gccacgatgc cgccgagcgc gcgctcgacc
     3181 tggtcaaaga tgtccacacc ccgccccgtc ggcccgcccg ggagaaaccg ctgcaggagc
     3241 tggtgcaccg agatctggtg cgcggtgatc tgatgctgca ccaggtagcc gaccaccgcg
     3301 agcgcgagcg ccgcgaacgg gatggcggtg agcaggagat cgaacgacag tccgctcgcc
     3361 agaaacggga cgttgtcctc gaacgccgcc tgaaaggtgc ggcgcaccat gcccacgaac
     3421 gattggtgcc acccaccctc gaccttcact cgagtggcgt atccgcagtc cccgctccgg
     3481 cgcctggaag ggtgcgccgc gcggctcggg ccgcgtgcaa gcgccggcgc agttgctccc
     3541 gcggagacgc atcgggctct tccgtcgcca tcaccgtcct gagctcatcg acgatgtccc
     3601 ccaggtcctc gcggagctgt cccgcgcgct tcccgacccg ggcccggatc ttcgatcccg
     3661 actccggcgc gaagagcgct ccgagcgccg cgccgatggc gaggccgacg agaaacgggc
     3721 cgaccgccga gcggtcgggg gtgccctcgt cgtcgctcac tcacccgcct cgagatcgtc
     3781 gggagccgcc accgccgcca tgcgaggcgc cgcgtgagag tcgaggggcg gcttcacgaa
     3841 ccggtccgtc gcggcgcgcg tgttccccac tcgcagttgc tcgaagagaa atgcaaacgt
     3901 cttctcgaga tcggcgccga tcgcctgacg aatgtcggcc ggcaggtccc agaatttctt
     3961 cttgaacgga ccgatcgcct tcttccggct cttgtagatg aactgctctt cggtgtcccc
     4021 cttcttccac tcctccttgc actccgcctt cacccacgcg ttcacctcgg ccctgagcgc
     4081 ctgaggcagc ttggggtgat cgaacaccat ggtctggaag ttcgtggcga ggtggatctc
     4141 gaccgcctca cagagcggga agttgctgaa ggcgtcgggg ggcagcgtcg acgcgccgtg
     4201 ctggaccgcg ccgccgagtc cgtagatcga gcgcgcttcc tgcgagagct ccttcaaggc
     4261 cgcgaggtcg agctgcacct tcgcgatggt cccatcgggc agtacgacgc cgccatgact
     4321 cgtgcccgtc tgcaccgaaa tcttgctgat gccgtgcttc accccgaggt ggtggcgcgt
     4381 gcgctcgtag ccgcccatga acgccttgag ctcgtgcacg tcggaatttt tcccgcccac
     4441 ttccccgatt tcggcaccga cggagatcgt gacgcccttg ggctcgcgct gccggatgta
     4501 ctgggtcagc tccgcggcac gctcgaaatt cacgcgctgc tgctcgtcca gcgtcttctg
     4561 ggagagatcg accagcgtcg acgtatcgac gtcgatgttg tagaagcccg cgtgaatctc
     4621 ttcgtcgatc agcgccttga tcgccgacag ctcggccgcc gggtcggtcg cgtacttctt
     4681 ggcgttgacc tggacgtgat cgccctggat gaagatcggg tggtcgaacc cctcgcgcag
     4741 cgcggcggcg gtgatcacgg cgacgtattc cgccggacgc tgctccgtgt acccgatctc
     4801 cgagcgcgcg atttcacaga tcacggcgcc gacgtccatc cggacggccg cgcggaaaat
     4861 ggcgcgcgcg gtgtcgtagc ccatcatccg gacgttgatc gcgggcgtcg tgaacgccga
     4921 gacctccccg cgtccacggg ccaggtacag ctcgtggatc gacgcgggac gagccccggc
     4981 ctgctgcccg atctcccaga tcagccagcg cgcccggtcg cgggtcccct ggtccccgaa
     5041 caccgcgtcc cggaccagct ggtccatggc atccgagcgc cgtaccgaag gcgacgcaaa
     5101 gcggcgcagg atgtcctgtg gctcctcagc catccgtaaa cctcctgtat ccttgaattt
     5161 aggactcgcc tacgtcgatg accagaccgt cgtgcgccgg cgcgatgccc gcgggcaacg
     5221 aagcggccaa gtccgcatgc ggggtctcgt gggtcaggtg ggtgaagaac gtacggcggg
     5281 ccccgatcgc ctgcgccacc gccaccgcct ccggtaccga gagatgcagg gggtgaggcc
     5341 gcgacaggag cgccgacagc acgagcacgt cgaggccccc caggcgggtc ctcgccgcct
     5401 ccggcacgtc cttcacgtcg gtgacgtacg cgagacggcc gatccggtac gccagcactt
     5461 cctgccggcc atgcggcagc gccaagggct cgacgtcgag gccggcgatg cgaactgaga
     5521 cgccggggac gagcggacgt gcgacgagct cgggcttgga cgtccccgcc ggcgccttcg
     5581 actcgagatc gaagatgtac gggaatttcg cgccgagctc cgccagagtc tcgcgggagc
     5641 cgtaggcctc gaggggcgcg cgctgcttga cggtgaaggc gcggagatcg tcgatcccgt
     5701 ggacgtgatc ggcgtgcgcg tgcgtgaaga gcacggcgtc gagccggtcg acgccggccg
     5761 cgaccagctg caaccggatc tccggcgggg tatcgatgag aatgcggcgg tcgccgtctt
     5821 cgatcagcgc cgcggtgcgc ccgcggcggt ccttcggatc agtcgaggtg caggtccggc
     5881 agtgacagcc gatttgcggg accccgaagc tggtcccggt gccgaggagg gtcagccgca
     5941 ctcagacttc ctcgaccttg atcaggttgg tggaccccga cacctcccac ggcacacccg
     6001 cggtcatcac gatgcggtcg ccggggcgcg catactcccg cttgaggatc aggtcccgca
     6061 cgaccgcgag catcgcatcg tatccgggca cgcggtcgac gagcacgggc gtgatgcccc
     6121 acaccagcgc cagctggcgg aacgtgtgct tcagcgtcgt gaccgcgagg atcggtggcg
     6181 ctgggcgcat ggccgcgacc ttgcgcgccg tgaacccgcc ggacgtgagc gtcacgatga
     6241 gcggcgtgcg aagcagccgg gcgaccgccg aggtgcccag cgcgatcgcg tcctcgaccg
     6301 tgacgtttcc ccgcgaggac aggcgctgct cctgccgggg cgggaggtgg cgctccatct
     6361 cgcggatgat ccggtccatc gcgcgcaccg actcgaccgg atactcgccc gccgcggtct
     6421 ccgcggagag catcacggcg tcggtcccat cgagaatcgc gttggcgacg tcggacgcct
     6481 cggcgcgcgt gggccgcggc gcgtgcacca tggattcgag catctgggtc gcggtgatca
     6541 ccggcttccc caccgcgttg gcgtcccgga tcaggcgctt ctgcaccagc gggacctcct
     6601 cgaagggcag ctccaccccc agatcgccgc gcgcgaccat caccgcgtcg caggcactga
     6661 gaatgcgcgg gagatcgtcg agggccgtgg ccttctcgat cttcgccacg agcttcatct
     6721 cggacggcac caggagccgc agctccgcga gatcatcggc gcgccggacg aacgacagcg
     6781 ccaggaagtc ggcgccatgc tgaaccgcat gcgccacatc ctcgcgatcc ttgtcggtga
     6841 gcgcgggagc actgacctga agcccgggca ggttcatgcc cttgtggctg gtcagggtcc
     6901 cgccgtcgac cacgcgcgcg tgcacgcggg gcgccaccac cttcgtgacc tccaccgaca
     6961 ggagaccgtc gttcagcagg atgcgcgcac cgacccgcgc gtcggcggcc agctcgtcgt
     7021 aggtcgtcgg aatctcggcg cccttcgcca cgtcctccgg cgcgaagacc acctcggccc
     7081 ccggtacgag ctcgacgggt gccttcaggt caccgacgcg gatgcggggt ccttgcaggt
     7141 cgaccagaat ggcgaccgcc agcccgcgtt ggtcggcgat ccggcggacg gccttcatcc
     7201 actggccccg cacttcgggc gtgccgtgcg aggcgttgat gcgcacgacg ttgacgcccg
     7261 catcgaggag ctggccgatc aattcgtccg actgcgtggc cggaccgagt gtggcgacga
     7321 tcttggtgcg tcgaatcacg gttcctcccg gacgggaaga tatccccctc gaggccggga
     7381 ggccccgccg ctctgccagc ccctctacgc ccgccatacc gtctcccctc ccgccaccgt
     7441 cagcaccgcc tgagctgacc ggaacgccgc cccatctccc gccacacccg ccagcgccgg
     7501 tggcgaccag gcgaccaagt ccgccacctc ccccggcgcg agcgttccac ccgctccacc
     7561 ccgccccgct gccacccagg gcgccgccgt ataggcatgg agtacctcgt cgaacgacag
     7621 ccgctccgtc gcacgccacc cgccggccgg aagaccatcc cggctgacgc ggtccagcgc
     7681 ggcgtacacg ccgtcacgag gatcgatggt ttccacgggc acgtcgctcc cgaataccag
     7741 cgccgtcccg gccgagcgga gagaccggaa ggcgtaaacc cccgcacccc gatcacccca
     7801 gtgcttgtcc acgaggggaa tatcggtaag gaggtgtgcc ggctgcatcg aggcgacgat
     7861 cccggcccgg gcggcccgtt gcagatcggc ggggtgcacc atctggaagt gctcgatgcg
     7921 gtgcgcgatc gccacggggg gcagcgtctc gatgatgtcc agcgcccgcc gaaccgccgc
     7981 gtccccgatc gcgtgaaccg tcgcggcgat tcccccccgc gcggcccggg tcagggcctc
     8041 acgggcgtcc gcctcgctca tcagggcgat gccgcggtcg gccgagccct cgtacggatc
     8101 gaggagcagc gcggtctggc tcccgagcgc cccgtccaga aagagcttca ccccgccggt
     8161 caccagccat tcggaccccc agccgctccg cagacccacc cggagcgcgg catcgagccc
     8221 gtcggccggc aggtgggcca gaacccggag acgcagggct ccctcttcct cgagtctttg
     8281 gaaggcggtc agcgcgtcgg gcccttcgac gttctggatc cccgtgacgc cgagctggtg
     8341 ggcggccccc tgggcccccc ggaccgcctc caccacgcgt tcccgaccga cctggggcag
     8401 gtgccggtgc accagacgga cggcgtgctc cagcaggaga ccagttggct ccccggcagc
     8461 gtcgcggacg atccgtccgc cctcgggatc ccgagtgctg cggtcgatcc cgcaccgtgc
     8521 aagcgcggtg gagttcaccc aaagcgagtg cacgtcatgc gagtcgaagg ccgccggagt
     8581 gttggggagg acctggtcga gagcctcgcg ggtgggagga gcctcccagc cgttggggtc
     8641 gaacccctga ccctgaatcc acccatcagt gagaaccccc ttcgccgccc gagccactgc
     8701 ctccgcgcgg gtccggcacc cgttcaggct cacccgctgg cgcccgatgg cccactcggc
     8761 gaagtgggtg tggccatcca cgaaccccgg cgtgaccaga cagtccggaa gctcgatcag
     8821 ggtgggtggc ggtccacctg cgaccgtggc ggcgcttcgg acgtcagcga aggtgccgac
     8881 gccttcgatt tttcccccgc gccaccacac tgcgtcgccg gtgcccccgg ctgagaccac
     8941 catcttggct cgtaggaggg tggagggagg gctcggagct tgggttttca acattccttc
     9001 cagatcagcg aggtgtggat aactcgcggt tcaggtgacg atttgttgac cgcgacgaac
     9061 acgcacgtga cgagtccgta agtggttgtt caagaagacc gtgcgatgac ggcgttcatg
     9121 cgacttctca cggcgcatag ccctgcatac gcctgtataa agatacgcac acgtgaatag
     9181 agcctaccgg agaacctcga tctggcgccc ttccagcctg cggcggtact cctggagcac
     9241 gtggatggtc cgctggatgg cggtggcggg catgccttcg atggtgcgag ccgagagacg
     9301 gcgctccagc tggccgaccc gcaggagcac ctcgaggccg tgccgcgtga gggtgacgcg
     9361 aacgccacgg tcatcgtcct ggtctggggt ggtctcaacc aggcctttcg cttcgaggcg
     9421 ctggatgaac gtcgtggccg cagccctgct gatcccgagg gcctccgcga cctcccgggg
     9481 cctgatcccg tcgggtccac cctgatcgac ggcgtagagg gccgtgtggg cccgatgcgt
     9541 gagcccgtag gtgcggaagg cacggtcccc cgaccgctcg acggcggatt gggccgcggt
     9601 cacggcg
//
LOCUS       DASUBP010000124         4600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_466356, whole genome shotgun sequence.
ACCESSION   DASUBP010000124 DASUBP010000000
VERSION     DASUBP010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4600
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_466356"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VFU75_04670"
     CDS             <1..273
                     /locus_tag="VFU75_04670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5466159.1"
                     /translation="YVLRVRFPAGLHIQPHYHMDNRIVQVLSGTMYFALGDSGDTTRM
                     RAIPVGSLWTEPARMVHYAWTGNGEVVLQVVGTGPTATQQVTLVKP"
     gene            complement(591..1688)
                     /locus_tag="VFU75_04675"
     CDS             complement(591..1688)
                     /locus_tag="VFU75_04675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HEU5466160.1"
                     /translation="MESWQELLRKKSIASLEVLAARFGAEHFPELDLLRQAAENFEFR
                     ISPAMVDLIKSPDDPIWRQYVPSVQELEVHDGIVDSLAEDEHSPVPNITHRYPDRALF
                     LVSPVCASYCRFCTRRRKVGDPEKIPMSQLESAFQYLEQHTEIRDVIMSGGDPLLLSD
                     RRIDDILKRLRAIPHLEVIRIGSRVPCHLPERITPELCAILKKYHPFYINTHFNHPDE
                     LTPAAVKGLGMLADAGIPLGCQTVLLKGVNDDPAVMKKLMQKLLAARVRPYYIYMCDQ
                     VAGAEHFRTTVEKGLEIVKALRGWTSGLAVPHFVIDAPGGGGKIPLLPEYVVKITDKE
                     VVLRNYTGKRFKYKQPYVPEPAPELEPILVG"
     gene            complement(1660..2160)
                     /locus_tag="VFU75_04680"
     CDS             complement(1660..2160)
                     /locus_tag="VFU75_04680"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5466161.1"
                     /translation="MNCSLSPVGRGHRERLERLTRETGFFREEEVATAVELLDEHLDG
                     DEDYRFIGAFAGNELTGYACWGPTPGTEGTWDLYWIVVARERQGEGIGSSLLNHVEQT
                     LMADRCRLVVVETSSRADYAPTRGFYEARGYTRTAVIPEYYAPGDDLVIYLKDLTHGV
                     LARTAP"
     gene            complement(2123..3130)
                     /locus_tag="VFU75_04685"
     CDS             complement(2123..3130)
                     /locus_tag="VFU75_04685"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU5466162.1"
                     /translation="MKVVILYDPGAPDWTTEDIAGVMKAVDEIGVIFTDMGHQVQKVG
                     VRHDLKWLQTSKRADLVFNLCEGVHGVAVWEDHVVAMLEFEGIPYTGCGSWTVAACRR
                     KTVANAILLQAGLPIPQWTTTHDRIPDDFPLPAIVKPAAEDASAGLDRGSVVTDRKAL
                     RTRVAAMTEQFDEVLVQQYIAGREFNVGFVGTRVLPVSEIDFTGMPEGAWPILTYAAK
                     WHAGSAEDLGSQPVVPAPISQRLSERLVRVAEKAWQTMQGKGYGRVDMRVDDTGRPWV
                     LEVNPNPDLTDEAGLSRMAKAAGWDYAELVRRVAEVALREAQGAKAARELLAQPSRSR
                     A"
     gene            complement(3127..4182)
                     /locus_tag="VFU75_04690"
     CDS             complement(3127..4182)
                     /locus_tag="VFU75_04690"
                     /inference="COORDINATES: protein motif:HMM:NF019121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466163.1"
                     /translation="MRIGLAYNEKPRSALGADDPASTTDAYVEWDEPSTIDAVEQALS
                     LFGSVIRLEADGTFPQKLALARPQIVFNMAEGLSGPNREAHVPAICEYFGVPYTASDP
                     LTLSLTLHKARTKEILAFRGIPTAPFALISAPEDLDRVTLPFPLFVKPVAEGSGKGIF
                     ANNLCESEGQLRERTLFLLDEYRQPVLVETYLPGREFTVAILGNGPEARCLPIVGFDF
                     TTLPQGATPVYGYEAKWIWDDADHQLEIYECPARVPADLRAAIERVSLDAYHALGCRD
                     WCRIDVRLDVEGVPNVVELNPLPGIIPDPKMNSCMPKAARVAGMTYDQLIQEVVRMAW
                     KRIHGRDLVVGAEEAIA"
     gene            complement(4378..>4600)
                     /locus_tag="VFU75_04695"
     CDS             complement(4378..>4600)
                     /locus_tag="VFU75_04695"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HEU5466164.1"
                     /translation="NATEVKIVVSGNARAWRTMLELRTGEGAELEIRRMAVACLAALS
                     KDAPAFFSDFETYKADDGGESARVVYHKV"
BASE COUNT          797 a         1465 c         1484 g          854 t
ORIGIN      
        1 tacgtgcttc gcgtccgatt cccggccggt ctccatattc agccgcacta ccacatggac
       61 aaccggattg tccaggtgct ctccggtacg atgtacttcg cattgggcga ttcaggggac
      121 acgaccagga tgcgcgcgat accggtgggc agcttgtgga cggagcccgc gcgcatggtc
      181 cattacgcgt ggaccggtaa tggtgaggtc gtgcttcaag tcgttggcac ggggccgacc
      241 gcaacccagc aggtgacact cgtgaaacca taggctagtc ccgccggaac gatggcggcg
      301 gtgaagtgtc cgaaggtggg cagcgccact tcgacccgga ggcagcgtga gcgatcctca
      361 gtggggtcgg gtccaacccg agatttgtgc tcaatgtggc catcccttcg atgaccacct
      421 cgtttccggc gaacttccat cgcccacggg gggcacgatt acctgcccgg tgccaggctg
      481 ttcgtgcttc ggaacctgga gccttgcttt agacgcttcg tctgacgatg acgcaaaacg
      541 ggaaacgtgg tagccacgtt tcccgttttg cgcttgttgc ggcctgagac ctagccgacc
      601 agaatcggct ccaactccgg cgccggttcc ggcacgtagg gctgtttgta cttgaatcgc
      661 ttgcccgtgt agttcctcag gaccacttcc ttgtccgtga tcttcacgac gtactcgggg
      721 agcagcggaa tcttcccgcc gcctccgggg gcgtcgatca cgaagtgcgg gaccgcgagg
      781 cccgaggtcc agccacgcag cgccttcacg atctccaggc ccttctcgac cgtggtccgg
      841 aagtgctccg cacccgccac ctggtcgcac atgtagatgt agtagggccg gacgcgcgcg
      901 gcgaggagct tctgcatgag cttcttcatc accgccggat cgtcgttcac acccttgagc
      961 aggaccgtct ggcaccccag gggaatcccg gcgtccgcca gcatgcccag gcccttcacc
     1021 gcggccgggg tcagctcgtc cgggtggttg aagtgcgtgt tgatgtagaa cggatggtat
     1081 ttcttgagga tcgcgcagag ctccggcgtg atccgctcag gcaaatggca ggggacccgg
     1141 ctcccgatcc ggatgacctc gagatgggga atggcccgga gccgcttcag gatgtcgtcg
     1201 atccggcggt ccgagaggag caggggatcg ccaccggaca tgattacgtc gcggatctcg
     1261 gtgtgctgct cgaggtactg gaaggccgac tcgagctggg acatcgggat cttctcgggg
     1321 tctccgacct tgcgccggcg ggtacagaac cggcaatacg aggcgcagac ggggctcacc
     1381 aggaacagcg cacggtccgg ataccggtgc gtgatgttcg gcaccggcga atgttcgtct
     1441 tccgctagtg aatccacgat cccgtcgtgc acctcgagct cctgcacact gggcacgtac
     1501 tgacgccaga tgggatcgtc gggggacttg atcaggtcca ccatcgcggg cgagatccgg
     1561 aactcgaaat tctccgctgc ctgcctgaga aggtcgagct ccgggaagtg ctcggcgccg
     1621 aagcgggcag ccagcacttc cagggacgcg atgctctttt tacggagcag ttcttgccag
     1681 gactccatgt gtcaggtcct tgagatagat caccaggtca tccccgggcg catagtactc
     1741 cgggattacc gcggtcctgg tgtacccgcg ggcctcgtaa aacccacgcg tcggcgcgta
     1801 gtccgcgcgc gatgacgtct ccacaacgac aagccgacag cgatcagcca tcagcgtttg
     1861 ctcgacatga ttgaggagac ttgagccgat cccctcaccc tgccgctccc gggccaccac
     1921 gatccaatag aggtcccagg tgccttccgt accgggtgtc ggaccccagc acgcgtatcc
     1981 cgtcagctca ttcccggcga aggccccgat gaaccggtag tcctcatcgc cgtccagatg
     2041 ctcatccagg agctcgaccg cggtagcgac ctcttcctcg cggaaaaagc cggtctcccg
     2101 ggtcagacgc tcgagtcgtt ctctatgccc gcgaccgact gggctgagcg agcaattcac
     2161 gggccgcctt ggctccctga gcctcacgca gggccacctc ggccacccgg cgcaccagct
     2221 cggcgtagtc ccaccccgcg gccttggcca tccgggacaa cccggcctcg tcggtcagat
     2281 cggggttcgg gttcacttcg agcacccacg ggcgcccggt gtcgtccacc cgcatgtcca
     2341 cccggccgta tccctttccc tgcatggtct gccacgcctt ctcggcgacg cggaccaggc
     2401 gctcggacag acgttgggaa atcggcgcgg ggaccacggg ctggctcccc agatcctcgg
     2461 cggagccggc gtgccacttg gcggcatagg taaggatggg ccacgcacct tcgggcattc
     2521 cggtgaagtc gatctcggag accggaagca cccgggtccc gacgaacccg acgttgaact
     2581 cgcgcccggc gatgtactgc tggaccagga cctcgtcgaa ctgctcggtc atcgcggcga
     2641 cgcgggtccg cagcgccttc cggtccgtga ccaccgatcc acggtcgagg cccgcactcg
     2701 cgtcctcggc cgccggcttc acgatggcgg gcagggggaa gtcgtccggg atgcgatcgt
     2761 gggtggtggt ccactggggg atcgggagcc cggcctgcag caggatggcg ttcgccacgg
     2821 tcttccggcg gcacgccgcc acggtccagg agccgcaacc ggtgtatggg atgccctcga
     2881 actcgagcat ggcgacgacg tgatcctccc agactgcaac tccgtgcact ccttcgcaca
     2941 ggttgaagac caggtcggcg cgtttggacg tctggagcca cttgagatcg tgccggacgc
     3001 cgactttttg aacctggtgg cccatgtcgg tgaagatgac gccgatctca tcgacggcct
     3061 tcatcacgcc cgcgatgtcc tcggtggtcc agtccggagc gccggggtca tagaggatga
     3121 cgaccttcac gcgatcgcct cctcggcacc gactaccagg tcacggccgt ggatccgctt
     3181 ccaggccatc cgtaccactt cctggatcag ctggtcgtag gtcatcccgg cgacccgcgc
     3241 cgctttgggc atgcacgagt tcatcttcgg gtccgggatg atgccgggga gcggattcag
     3301 ctcgacgacg ttcgggacgc cctcgacgtc caggcggacg tcgatccggc accagtcgcg
     3361 gcagcccagc gcgtggtaag cgtcgaggga gacccgctcg atggcggcgc ggaggtcggc
     3421 cgggacacgg gccgggcact cgtagatctc gagctggtgg tcggcgtcgt cccagatcca
     3481 tttggcttca tagccgtaga cgggggtggc gccctgtggc agggtggtga aatcgaatcc
     3541 gacgatcggc aggcaccggg cttccgggcc gttgccgaga atggcgacgg tgaattcgcg
     3601 gccgggcagg taggtctcta ccaggaccgg ctggcggtac tcatctaata gaaagagtgt
     3661 ccgctcccgg agctggccct cggactcaca taagttattg gcaaatatgc ctttacctga
     3721 cccctccgct acgggtttca cgaagagcgg gaagggcagg gtcacccggt ccagatcttc
     3781 gggggccgag atcaaggcaa agggcgctgt cgggatccct cgaaaggcga ggatctcctt
     3841 ggtgcgcgct ttatggagcg tcagcgagag cgtgagtggg tcggaggccg tgtaggggac
     3901 gccaaagtac tcgcagatgg cgggcacatg cgcttcgcga ttcgggcccg agagcccctc
     3961 ggccatgttg aagacgattt gcgggcgggc gagggcgagt ttctgcggga aggtgccgtc
     4021 ggcttcgagc cggatgacgc tgccgaagag actgagtgcc tgctccaccg cgtcgatggt
     4081 ggaaggctcg tcccactcga cgtacgcgtc ggtggtactg gcaggatcgt ccgctcccaa
     4141 ggcggatcga ggtttctcgt tgtaggcgag accgattcgc attggtctcg gattacctcc
     4201 tacaaaaaaa ggccgcacta tgtcggcggt ctaactgcat tcaacccgat gtcaatgagg
     4261 acaataggat gattcggcag gatgcgcaat agctagaacc aaattcggaa gtcggaactc
     4321 ggaacgcgga acatcatgtt gtcggatccg cattccgact tccgcgttcc gcgttcctca
     4381 gaccttgtga tacaccaccc tcgctgattc tccaccgtcg tcggccttgt acgtctcgaa
     4441 gtcggagaag aacgcgggcg cgtccttcga cagcgcggcg agacacgcga ccgccatgcg
     4501 tcggatctcg agctcggcgc cctctccggt acgcaattcg agcatcgttc gccacgcgcg
     4561 ggcgttcccg gacaccacga tcttcacttc ggtggcgttg
//
LOCUS       DASUBP010000125         6223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1338459, whole genome shotgun sequence.
ACCESSION   DASUBP010000125 DASUBP010000000
VERSION     DASUBP010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6223
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1338459"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1493)
                     /locus_tag="VFU75_04700"
     CDS             complement(<1..1493)
                     /locus_tag="VFU75_04700"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEU5466165.1"
                     /translation="MPQLKRVLVANRGEIALRIIRACHEEGLEAVAVYSDADRLSPHV
                     RAANRAVHLGAAPAAESYLNIPKLIAAAKDTGCQAVHPGYGFLSERAPFSAAVRDAGL
                     TFVGPSPEAIKAMGDKTEARRRMQHAGVPIVPGLTTPLKDAGEAAREAQRIGFPVLFK
                     AAAGGGGKGMRLVRSAAEAESAFEAARSEAQKAFGDGAVYIEKYIEQPRHVEIQILGD
                     THGRVVALGERECSIQRRHQKLIEESPSVAVSPALRRRMGDAATAAAGSVGYVGAGTI
                     EFLLASNGEFYFLEMNTRIQVEHPVTELVFGVDLVREQLRIAMGHPMRVHRGALEPRG
                     HAIECRITSEDPFNGFLPATGVVKYLRIPSGPGVRWDGGVEAGNEVTLHYDPLIAKLI
                     VWGENREVALARMGRALRELVIVGLPTSQAFHLRVLADPDFRSGAIDIGYLDRKGEAL
                     LSAPLSDALVKPVAVVAAMLADERRSGHAAESTAPGSRLPTSPWLDAA"
     gene            complement(1481..2434)
                     /locus_tag="VFU75_04705"
     CDS             complement(1481..2434)
                     /locus_tag="VFU75_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466166.1"
                     /translation="MAAFLFNALLAAQLVQAQVPTPDSLAVLASRVARDSGDAAAWLA
                     LGRAQLRAAEAFHTHAGPPDSGWARGEILSAEMAFGRAVQLRPGLGLGDSASTYRVVA
                     RQDLATLTWELGDTAAVDSVWQEMPSGSRLPPVLEELAENLLRACPPHAVLFTDNEVV
                     SAAAAYLRFNHAIRPDLLAVPMSRYRSDSVFAARVAKDAGLKKAPGKHENAEARVLAL
                     GALRPVCAGVDLGSPPGGHGRIAWQTRPFVWITGKNAGASPPVPPADFVFAALKFAMD
                     ANDPWARFALDLYRHAARLSPGLCQPLASYGIPRARTGCRS"
     gene            complement(2415..3938)
                     /locus_tag="VFU75_04710"
     CDS             complement(2415..3938)
                     /locus_tag="VFU75_04710"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013935707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HEU5466167.1"
                     /translation="MSTRQKLEDLQRRRAESEQGGGAKRIEAQHKKGKMTARERLDVL
                     LDPGSFVELDRFVTHRINDFGLADQKVPGDGVVTGYGRVDGRLVYVFSQDATVFGGSL
                     SEAHAEKICKVMDSALKVGAPVIGLNDSGGARIQEGVASLGGYADIFLRNTLASGVVP
                     QISAILGPCAGGAVYSPALTDFVFMVRGVSYMFVTGPDIVKTVTHEDVDFEGLGGADV
                     HGGTSGVAHFVHDAELESIAAIRTLLSFLPSNNLETPPIKATTDPVDRRDEALLDLIP
                     DTPNKPYDMRDVITRVVDDGDFFEVQRDYAQNLLVGLARLGGRPVGIVANQPAVLAGA
                     LDIGASLKGARFIRFCDAFNIPLVTFEDVPGFLPGVGQEHGGIIKHGAKLLYAYCEAT
                     VPKLTVITRKAYGGAYDVLSSKHIRGDYNVAWPTAEIAVMGAEGAVKILYKGESSDAR
                     IQEYRSKFAHPYQAASRGYVDDVIDPRDTRPRLVDALVALATKRDKNPPKKHGSIPL"
     gene            complement(4078..5781)
                     /gene="rpsA"
                     /locus_tag="VFU75_04715"
     CDS             complement(4078..5781)
                     /gene="rpsA"
                     /locus_tag="VFU75_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEU5466168.1"
                     /translation="MQVEAQDIEDSPVEHPKKSSTHSVLRVRADLHEEYSADDIAKMT
                     ELYEGTLSNIEEGEIVKSKVLRVTDAAVILDVGFKSEGAVPIDEFKDPKSLKEGDEIE
                     VFLEHLEDQEGAVVLSKKKADFMRVWEKIREAHEKDEAVHGTLIKKIKGGVVVDLMGV
                     DAFLPGSQIALRRVPNIDELLGQSFEFKIIKLNKRRRNIVVSRRVLLEAERRTKRDVL
                     MKELQVGQVRKGVVKNITDFGAFIDLGGVDGLLHITDMSYGRVSHPSEMVQIGQELEV
                     KILDIDWERERISLGLKQLQSYPWQNVAAKYPVGTRVQGKVVSITNYGAFVELEPGIE
                     GLVHISEMSWTRNVRHPSKVVSIGESIEAVVLKVDETEEKISLGMKQTEQDPWMALPQ
                     KYPVGTRITGKVRNLTSFGAFVEIEPGIDGLIHISDMSWTKRVQHPSEMLKKGDTVTV
                     LILNVDAENKRISLGLKQAEEDPWLRIGETYPVGTQMRGRVVRLMDKGVVIDLGNDIE
                     GFIPMSQLGIPDIQDPAEAVKEGQAVEVSVLEVDPIHHRIVLAATGFPKEETPSEQAG
                     A"
     gene            complement(5864..>6223)
                     /locus_tag="VFU75_04720"
     CDS             complement(5864..>6223)
                     /locus_tag="VFU75_04720"
                     /inference="COORDINATES: protein motif:HMM:NF014300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEU5466169.1"
                     /translation="RKWVNAQQRQAAKEHPSGVVVDGRDIGTVVFPDAPVKVFLTATP
                     EERVRRRVTQRGEALDPERIARESRALASRDEADSQRAVAPLKPAPDALILDSTRLSF
                     EEQVQRVVALAREWALR"
BASE COUNT         1018 a         2163 c         1990 g         1052 t
ORIGIN      
        1 gccgcgtcga gccacggact tgtcgggagc cgggagccgg gagccgtact ctccgcagcg
       61 tgccctgatc tccgctcgtc agccagcatc gccgccacca ccgccaccgg cttcacgaga
      121 gcatcggaca gcggagcaga gagcaacgcc tcccccttgc gatccagata cccgatgtcg
      181 atcgcccccg agcggaagtc gggatcagcg aggacgcgga ggtgaaacgc ctgcgacgtg
      241 ggcaacccga cgatgaccag ctcacgcagg gcccggccca tccgcgcgag ggccacttcg
      301 cggttctcac cccacacgat gagcttggcg atgagcgggt cgtaatgcag cgtgacctca
      361 ttcccggcct cgacccctcc gtcccaccgc acgccaggtc cactcggaat tcgcagatac
      421 ttcaccaccc cggtggccgg caggaatccg ttgaacggat cctcggacgt gatccggcac
      481 tcgatggcgt gcccgcgcgg ctcgagcgca ccgcgatgca cccgcatcgg gtgccccatc
      541 gcgatgcgca gctgttcccg caccaggtcc acgccgaaca ccagctcggt gacgggatgc
      601 tcgacctgaa tgcgggtatt catttcgagg aagtagaact cgccgttcga cgcgagcaga
      661 aactcgatgg tcccggcgcc gacgtacccg acggagccgg cagcggccgt cgccgcatca
      721 cccatccggc ggcgcagggc cggtgacacg gccaccgacg gcgactcttc gatcagcttc
      781 tggtgacgcc gctgaatgct gcactcgcgc tcccccaggg ccaccacccg gccatgggtg
      841 tcgccaagaa tctggatttc cacgtgccgg ggctgctcga tgtacttctc gatgtagacg
      901 gcgccgtccc cgaacgcctt ctgcgcctca ctccgcgcgg cctcgaacgc cgattcggct
      961 tccgccgcgc tgcgcaccag gcgcatgccc ttcccgcccc cgccggccgc ggccttgaag
     1021 agcacgggaa acccgatccg ctgagcctca cgcgccgctt cgcctgcgtc cttgaggggc
     1081 gtggtgagcc ccggcacgat cggcaccccg gcgtgctgca tcctgcgccg ggcctccgtc
     1141 ttgtcaccca tcgccttgat ggcctcgggt gacggaccga cgaacgtgag cccggcgtcc
     1201 cgcaccgcgg ccgagaaggg cgcccgctcc gagagaaagc cgtacccggg atggacggcc
     1261 tggcatcccg tgtctttggc ggcagcaatc agtttcggga tgttgaggta ggactcggcc
     1321 gccggcgccg cacccaggtg cacggctcga ttcgccgccc gcacgtgggg cgacaggcga
     1381 tcggcatccg aatagacggc gaccgcctcc agcccttctt catggcaggc ccggatgatc
     1441 cggagcgcga tttcgccccg gttggcgacg aggacccgct tcagctgcgg catccggtcc
     1501 gggctcgggg gatgccataa ctcgccagcg gctggcagag gcccggcgac agccgggcgg
     1561 cgtgccggta caggtcgagc gcgaaccgcg cccacgggtc attcgcgtcc atcgcgaact
     1621 tcagcgccgc gaagacgaag tcggcgggag gcaccggcgg gctggccccg gcattcttcc
     1681 cggtgatcca cacgaagggc cgcgtttgcc acgcgatgcg gccatgcccc ccggggggcg
     1741 atcccaggtc gactccggca cagacgggcc gcagggcgcc gagcgccaga acccgagcct
     1801 ccgcgttctc gtgtttcccc ggcgccttct tgagtccggc atccttcgcg acgcgcgcgg
     1861 cgaacaccga atccgaccgg taccggctca tgggaacggc caggagatcg ggcctgatgg
     1921 cgtggttgaa gcgcagatag gccgcggcgg ccgacaccac ttcgttgtcg gtgaacagca
     1981 cggcgtgcgg tggacaggcg cgcagcaggt tctcggccag ctcctcgagc accgggggca
     2041 ggcgcgatcc cgacggcatc tcctgccata ccgaatccac cgcggcggtg tcgcccagct
     2101 cccaggtgag cgtcgcgaga tcctggcgcg ccaccacccg gtacgtggac gccgaatcgc
     2161 cgagtcccag ccccggccgc agctgcaccg cccgcccgaa cgccatctcg gccgacagga
     2221 tctcgccgcg cgcccaaccg ctgtcggggg gtcccgcgtg ggtatgaaag gcctcagcgg
     2281 cccggagctg cgcccgcccc agcgcgagcc aggccgcggc gtccccggaa tcccgcgcga
     2341 cccggctcgc caggacggcg agcgagtccg gcgtcggaac ctgcgcctgg accagctgcg
     2401 cggcgaggag cgcgttaaag aggaatgctg ccatgctttt tcggcggatt cttgtcgcgc
     2461 ttggtcgcga gtgccaccag tgcatccacc agcctcggcc gggtatcccg cggatcgatg
     2521 acgtcgtcca cgtagccgcg tgacgcggcc tggtacggat gcgcgaactt ggagcggtac
     2581 tcctggatcc gcgcgtcgct cgactccccc ttgtagagga tcttcaccgc cccctccgcc
     2641 cccatcaccg cgatctccgc cgtcggccag gcgacattgt agtctccgcg gatgtgcttg
     2701 gacgacagca cgtcgtacgc gccgccgtac gccttgcgcg tgatcacggt gagcttcggc
     2761 acggtcgctt cgcagtacgc gtagaggagc ttggccccgt gcttgatgat cccgccatgc
     2821 tcctgcccca ctccgggaag aaaccccggc acatcctcga aagtgaccag gggaatattg
     2881 aaagcgtcac agaaccggat gaatcgcgca cccttcagcg aggccccgat atcgagggca
     2941 ccggccagga ccgcgggctg gttggccacg atccccaccg gtcgtccgcc caggcgggcg
     3001 agaccgacga ggaggttctg agcgtaatcc ctctgcactt cgaagaagtc accgtcgtcc
     3061 accacccgcg tgatcacgtc ccgcatgtcg tacggcttgt tgggggtgtc gggaatcagg
     3121 tcgagcaggg cctcatcccg gcggtccacg gggtccgtgg tggccttgat cggcggggtt
     3181 tcgagattgt tcgacggcag gaacgacagc agagtgcgga tggccgcgat ggattcgagc
     3241 tcggcatcgt ggacgaagtg ggcgaccccg gaggtgccgc cgtgcacgtc ggcgcccccc
     3301 aggccctcga agtccacgtc ctcgtgcgtg accgttttga cgatgtcggg accggtcacg
     3361 aacatgtagg acacgccacg caccatgaac acgaaatcgg tgagtgccgg actgtaaacg
     3421 gcccccccgg cgcaggggcc gaggatggcg ctgatctgcg ggacgacgcc ggacgcgagc
     3481 gtattccgga gaaagatgtc ggcgtacccc cccagggagg cgacgccttc ctggatgcgg
     3541 gcgccccccg agtcgttgag gccgatgacg ggcgctccca ccttcagggc cgagtccatc
     3601 accttgcaga ttttctcggc gtgggcttcg gagagggagc cgccgaacac ggtggcatcc
     3661 tgcgagaaga cgtagaccag ccgtccatcc acgcggccgt agccggtgac gacgccgtcc
     3721 ccggggactt tctgatcggc cagaccgaaa tcattgatcc ggtgggtgac gaagcggtcg
     3781 agctcgacga aggaccccgg atcgaggagc acatccagcc gctcccgcgc ggtcatcttc
     3841 cccttcttgt gctgcgcctc gatgcgtttc gcgcccccgc cctgctccga ctcggcgcgg
     3901 cggcgttgga ggtcttccag cttctgtcta gtagacatgc gaaaaatcta gacggctgga
     3961 gggtccggcg gatagacagg cgcccaggcg ctcaggcgcc aagcgggtaa caggaaaaag
     4021 ggtcaggaga agcttctcct gaccctgact cctacatacc gctaggcgcc tgagcggcta
     4081 agcgcctgcc tgctcgctag gcgtctcttc cttggggaag cccgtcgccg cgagaacgat
     4141 cctgtgatga atgggatcca cctcgagcac ggacacttcc accgcctgcc cttccttcac
     4201 cgcctcagcg ggatcctgaa tatccggaat gccgagctgc gacatgggaa tgaagccttc
     4261 gatgtcgttg ccgagatcga tgacgacgcc cttgtccatg aggcgcacga cccgtccgcg
     4321 catctgcgtg ccgacggggt acgtctcgcc gatgcgcagc cacggatcct cttcggcctg
     4381 cttcaggccg agcgaaatgc gcttgttctc cgcgtcgacg ttgaggatga gcaccgtgac
     4441 ggtgtcaccc tttttcagca tctccgacgg gtgctgtacc cgcttggtcc acgacatgtc
     4501 ggagatgtgg atcaggccgt cgatgcccgg ctcgatctcg acgaaggcgc cgaagctggt
     4561 caggttgcgg accttgccgg tgatccgcgt gcccacggga tacttctggg gcagcgccat
     4621 ccacggatcc tgctcggtct gcttcatgcc cagtgaaatc ttctcttccg tctcgtcgac
     4681 cttgagtacc accgcttcga tggactcgcc gatcgacacg actttcgaag gatgccggac
     4741 gttgcgcgtc cagctcatct cggagatgtg gaccaggccc tcgatgccgg gctcgagctc
     4801 cacgaacgcg ccgtagttgg tgatcgagac gaccttgccc tgcacgcggg tgccgaccgg
     4861 atacttggcg gcgacgttct gccagggata ggactggagc tgcttcaggc cgagcgagat
     4921 gcgctcgcgc tcccagtcga tgtccaggat cttgacctcg agctcctggc cgatctgcac
     4981 catctcggac ggatgcgaca cgcggccgta actcatgtcg gtgatgtgga gcagtccgtc
     5041 cacgccgccg aggtcgatga acgcgccgaa gtcggtgatg ttcttgacca cgcccttccg
     5101 gacctggccg acctgcagct ccttcatcag gacgtcgcgc ttggtgcgcc gctccgcctc
     5161 gagcagcacg cggcggctga ccacgatgtt ccggcgccgc ttgttcagct tgatgatctt
     5221 gaactcgaag ctctggccga gcagctcgtc gatgttcggg acgcgccgca gggcgatctg
     5281 ggacccgggc aggaacgcat ccacccccat cagatccacc accacgccgc ccttgatctt
     5341 cttgatcagg gtgccgtgga ccgcctcgtc cttctcgtgc gcttcacgga tcttttccca
     5401 gacccgcatg aagtcggcct tcttcttcga gaggacgacc gcgccctcct ggtcttcgag
     5461 atgctccagg aagacttcga tctcgtcccc ttccttcaag ctcttcgggt ccttgaactc
     5521 gtcgatcggg acggcgcctt cggacttgaa gccgacgtcc aggatcacgg ccgcgtcggt
     5581 gacgcgcagg accttggatt tgacgatttc gccctcttcg atgttcgaga gggtgccctc
     5641 gtacagctcc gtcatcttcg cgatgtcgtc ggcggaatac tcttcgtgga gatcggcgcg
     5701 gacgcggaga accgagtgcg tggaggactt cttgggatgc tctacgggcg aatcttcgat
     5761 atcctgcgct tcaacctgca tgtgatgacc accagttggg ttaggcgttt ccagaatcgc
     5821 atcccggggc caatccccgg ggcgtcaaac aaggaccggg aacctagcgg agtgcccact
     5881 cccgcgccag cgccaccacg cgctgcacct gctcttcgaa tgaaagccgc gtggaatcga
     5941 ggatcagcgc gtcgggggca ggcttgagcg gagccaccgc ccgctgcgaa tcagcctcgt
     6001 cccgggacgc cagtgcccgc gattcccggg cgatgcgctc cggatcgagc gcttcacccc
     6061 gctgggtgac gcgccggcga acccgctcct cgggggtggc cgtcaggaac accttgacgg
     6121 gcgcgtccgg gaagacgacg gtgccgatgt cccgtccatc caccaccacg ccggacggat
     6181 gctccttcgc ggcctgccgc tgctgggcat tcacccactt ccg
//
LOCUS       DASUBP010000126        24471 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_248609, whole genome shotgun sequence.
ACCESSION   DASUBP010000126 DASUBP010000000
VERSION     DASUBP010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 24471)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24471)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24471
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248609"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(127..3429)
                     /locus_tag="VFU75_04725"
     CDS             complement(127..3429)
                     /locus_tag="VFU75_04725"
                     /inference="COORDINATES: protein motif:HMM:NF013283.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HEU5466170.1"
                     /translation="MKHRWSQWVNEGSTGGGMKGTKVFLTGLACVALSTTASAQYFGQ
                     NKVQYRSFNWQIIQTEHFDVYFYPVEREAALDVARIAERSYARLSRILHHNFLERKPI
                     LLYASYSEFQETNALGGESPDEGTEGVTEFFKHRMVIPFTGDYEGMEHVIQHEMTHQF
                     QYDVYSGGHPGAGIQTMVAVNPPGWFMEGMSEYLSLGPLDPATAMALRDAALQGHLPT
                     IEDMTDNPNIFPYFYGHALFAYIGQRWGDEVIGEILQASRGAGIEGAFKRALGISLDE
                     LSKDWRDAVQATFLPEISSHYPARRIAEPLLTEKRSDGRLHLAPALSPDGKNIAYFGE
                     GNDFFIDLYLADAETGKTIRRLVKSTTSSNYESLRFIYSSGSFSPDGRYFAIAVKHKD
                     RDDLVLFDIKKNKEAARLKIPLNGINNPTWSPDGTQLAFTGYDGGLADLFIINRDGSD
                     LRRLTNDKYADLLPQWSPDGKTIAFSTDRGPNTNFQTLSTGNFRIALYHLDSGQIEVL
                     DHMEYGRNVNPVWAPDGRSLAFVSDRTGIPNIFLYDLGDKQVYQLTDVYTGVSGITPI
                     SPALSWAKETDRLAFAYYDDGSWDVYALDNPRSLRGRPYADKPMPPLVYAQTRPRYEM
                     ASLNADTGKITPASPSDTGASSVPSSSIYRAPSGLRQSGAQPAETTKVSAPVSVKQLL
                     DSNTLALPDTAEFSIMPYHVRFTPDYVARPTIGYERDNFGRGFFGGTAVSLSDMLGDR
                     TMVFSGAVNGRLAEAEVLAAYINQVHRTNWAVGFTQEPYYFYEPSTSSFDSAAVPPTY
                     VLNTNIRRFVIRDLFGTASYPFSQFSRVEFSLHTVAISDATLQIQDVYQPVCSGAFCP
                     VGENVNTIGGVTNAYAQPSAALVHDNTLFSYVGPFAGNRWRLEVAPAIGNWQFTAMTA
                     DWRRYFLFRPFTLAVRGLYFGRVGRDGGRFPTFLGSTELIRGYTAGSFQSNECVGVTN
                     GSSQTGCAELDQLIGSQVAVANVEIRFPLTRALVLGVLPLGFPPLEGAFFYDAGVAFN
                     SVSQLHWTRCATPSDPNCSPENVRVPLRSYGLGIRANMFGIVILRLDYTKPLDRPNNS
                     HAYWTLSLGPTF"
     gene            complement(3398..3772)
                     /gene="rsfS"
                     /locus_tag="VFU75_04730"
     CDS             complement(3398..3772)
                     /gene="rsfS"
                     /locus_tag="VFU75_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEU5466171.1"
                     /translation="MSSGRGRSRGKPAPALEGLRPILEGLSDRKAVQVVVLDLRGLSE
                     ATDYFVIASGTSDAHVRGMADHLMSLGGENKPHHVEGLTQGRWVLLDYVDFVVHLFHP
                     ELRDFYQLERLWGEAPVVAVGE"
     gene            complement(3765..5579)
                     /locus_tag="VFU75_04735"
     CDS             complement(3765..5579)
                     /locus_tag="VFU75_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEU5466172.1"
                     /translation="MPAGISYVDGPRLSRSLLAAADWVAAGRDELNRMNVFPVPDGDT
                     GTNMWLTLREVAQALRTLGDAPLPQVTETMAQASIRGARGNSGMMLSQFLLGLRDGLG
                     DRPTASPRDLARAIRTGFERLNGALDDPVEGTILTVARDAADEADAAADERDLKVFMR
                     RLVTRAELSLQRTPELLQALKSAGVVDAGAKGFVRLLDGVKRLIEEGGQAQGAVDRDL
                     VNAAATTEVASDRDYRFCTEVMVRGASLPKPEQVRAALRELGGSIVVLESGDLLKAHV
                     HTDTPDRVFELGRGWGTLEYTKAEDMRAQHQALVNKRAVAFVADTACDLPDSVVFEKQ
                     VGLVPMQVVVGDRVYRDRLELSAPEFFTRLRSGQDATTSQPTRQAFTEVYQDALRVAE
                     KIVVVTVSKPLSGTYANAEAAAKEMASDRISLVDSRSASLGEGMLVLRGLELAAQGVP
                     PGEIATELARVRTQSSGFFVVDKLDRLIRSGRVSRLAGWLGTRLNLKPILTLTPEGEV
                     DSAGRARGREAARTKVLALLDRALAARPQSLRLGVVHAGIHQFAETLRQELVRRYTPK
                     EALISPITPVLAAHTGIGTWGVFYQVEDGTNDGGRRYE"
     gene            complement(5590..6033)
                     /gene="rplI"
                     /locus_tag="VFU75_04740"
     CDS             complement(5590..6033)
                     /gene="rplI"
                     /locus_tag="VFU75_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEU5466173.1"
                     /translation="MEIILREDVQHVGKAGEVVKVKDGFARNFLLPKGLAYPATEGNK
                     KKITFEAARIMKVRAAEKDAAQVIADKLAQVQLTFSVKVGEEDKLYGSITAADIQRRL
                     AEAGHDVDKRQIDLPEPIRALGEFKVGVKVHADVRPEVQVTVVKE"
     gene            complement(6047..6940)
                     /locus_tag="VFU75_04745"
     CDS             complement(6047..6940)
                     /locus_tag="VFU75_04745"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HEU5466174.1"
                     /translation="MTGTLLLALFAPTSLPLAGLTFAGLQLIGGTRSSVFRAGALLMG
                     VLALWALVLPRETAVDAVIGVFQLFAAVAFVVMAWNAPGPFLPRAAGALAWSSASAVV
                     LAWILNGANTWGLLAWDTVRQTSFALRTVLEVMSSDVVADMYANYEPAVRLFSTVRPA
                     LEILWGLAALALAWQWHVRTARHPLGPALRPFREFRLADGAVWGVVAATIAWILSGGA
                     PGIRVAAVNVGLILGALYVLRGAAVVAAAAAMVAVPAWVQALAVLSGVLFAPATAPSL
                     CVLGVSDTWLEFRRRLEGRPN"
     gene            complement(6973..7194)
                     /gene="rpsR"
                     /locus_tag="VFU75_04750"
     CDS             complement(6973..7194)
                     /gene="rpsR"
                     /locus_tag="VFU75_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEU5466175.1"
                     /translation="MRRPSKTCPVCESGVRVLDYKDDRTVSRFLTERGKILPSRLSGM
                     CARHQRQLATAIKRARQLALIPYIKGFTG"
     gene            complement(7191..7547)
                     /gene="rpsF"
                     /locus_tag="VFU75_04755"
     CDS             complement(7191..7547)
                     /gene="rpsF"
                     /locus_tag="VFU75_04755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEU5466176.1"
                     /translation="MTRRYETVYIFDSALEEPAVTEKLDRIHALLTKDGKGSMTNVSH
                     WGKRTLAYPIKKKDTGYYVVAQFETTGDLLPEYERAVKLDEGVLRFLVVAAEAEAKPV
                     PAPVIGGDDDGEEDDA"
     gene            7686..8432
                     /locus_tag="VFU75_04760"
     CDS             7686..8432
                     /locus_tag="VFU75_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466177.1"
                     /translation="MWHFAALALSLLTADSTVLKGTVLSLGSGHPLAGVTVSRPGRAP
                     VTTDSAGHFAIPGPAARLHLTWNGVSGTGVIPAGAEDQSLLVVIDTGAADLSPTIEHE
                     DWHLAGHWGMPGFFGRARQGYGTFFTRDALVRSGFTNLKDLLSSLGIHHGCLRDGGGC
                     GAVIYYRNVPTLVSIYVDGAFQNTEQADDRSLGDVTGIEYYPLPNPPRPALASPREWI
                     VKTDYLEQLPLQEFTVVLWTRDFYQRLAER"
     gene            8503..9432
                     /locus_tag="VFU75_04765"
     CDS             8503..9432
                     /locus_tag="VFU75_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466178.1"
                     /translation="MLLLVGACHSAPAAPPPVPYVPVELRVTLDRSAYAEGDPVYALV
                     ELRNVGTDTVCVPSMSLDQDWLKMDLSRDGVRIAGGKMLWVDEFYSPGYLGEPLPPGG
                     AHYEPILLSERWSDGDRVSQALFLGRLPPGLYALVAQFNPRIHWWPGATVTSMPVAFI
                     VRSRDSAENARYDLWMRFAKQLQPGHWPAEVTDSLMGALAARVAGDSADPLSATLAEF
                     VATGEMVAQGRRDHIAIIREWQSAVMRHEPDAPAAAWAAARYGEWWLHGDTVPIRRLA
                     ESLHGTVAGGILDRSLARHSSPPTRCAPRTPIR"
     gene            complement(9422..10525)
                     /gene="ychF"
                     /locus_tag="VFU75_04770"
     CDS             complement(9422..10525)
                     /gene="ychF"
                     /locus_tag="VFU75_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEU5466179.1"
                     /translation="MLRLGIVGLPNVGKSTLFNALTSSKAAAAENYPFCTVDPNVGVV
                     EVPDPRLDQLVELVKPKKKVPAVVEFVDIAGLVKGASLGEGLGNQFLSHIREVDAIVH
                     VIRCFEDPDVVHVMGPVDPVRDHDVISAELALADVSVIDRRMDKTRKSAKSGDKDAQV
                     EMALLERVTTELDRGKGARAAAKDAMELAALRAMGLLTSKPILYAANVSEDDLAAGGG
                     KFVDVLKKAVAAHHEEAELVPFSAKLEAELAELARDERQEFLAGAGVKEPGLDRLIHA
                     GYALLGLRTYFTAGEQEVRAWTIHVNDTAYDAAGVIHSDFQKGFIRAETVSFADFTTA
                     GGWKPAREQGLVRSEGREYVVQDGDVLLFRFNV"
     gene            complement(10536..11642)
                     /locus_tag="VFU75_04775"
     CDS             complement(10536..11642)
                     /locus_tag="VFU75_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466180.1"
                     /translation="MNLVPWFPEIAILAGTLALIPALVALRAANRRPELGTFAEPGLV
                     AVVALVVLDGLISARTGASFALGAIAALSVQWMADFGIESRTLGLSAWGTSLLGLGLV
                     FELFGSAAGMDDGGWRLFAQLIAAFALGSSVISASRKSDSMVPEASDAGVFATAAALV
                     VAAATPFAANRLDGVAYPMLVATTGIFGGAVALLWKGSARTGLVTGAAVVGCLVALAA
                     TGLDPVALGLHEDTLSPQVTLLAGLGGVVLGAGITLINRRMVTIGLVVVAVASVHVVG
                     PYGAAVAALAAVTAVTLPSAPAQAVTVIAALALVVASHSGSPLVTPSHWSGWFHTFVA
                     AVVVGVIGAAGKGPRFALVRVAVLTWIVIAPFLR"
     gene            complement(11639..12184)
                     /gene="pth"
                     /locus_tag="VFU75_04780"
     CDS             complement(11639..12184)
                     /gene="pth"
                     /locus_tag="VFU75_04780"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEU5466181.1"
                     /translation="MRAVVGLGNPGEEYQATRHNAGFWLADALTARWGFGPFRRGERA
                     RVSDGEVAGVPVRILKPQTYMNRSGAALVSLRADPTFNPATDLLVLVDEFRIAAGTFR
                     LRLDGSAGGHNGLKSIEGALQSQAYARLRIGVGPLPEGVGDWADYVLAPADPEQRDLI
                     EAAMPQMVEAVERWLQGQPPA"
     gene            complement(12188..12910)
                     /locus_tag="VFU75_04785"
     CDS             complement(12188..12910)
                     /locus_tag="VFU75_04785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HEU5466182.1"
                     /translation="MAQIVSLTAQNRDQTGKGAARQSRFAKKVPAVVYGHGRAAQNLT
                     VDAPALEKALTGIEPESTLIELTIDGKKAKALIREIQRHPVRPDIIHVDFYEIHADEK
                     VKLKVPVHLVGTPDGVRNAGGVLDQVTREVEIEVLPEHIPDRVELDVSALIIGHSLHV
                     RDLVIPNATILTLADLTIAVVVPPRAEEVVAPAAEGATDVAEPELIRKEREDEEVEGE
                     EGKPAAEAKGEAKPKGEAKPKG"
     gene            complement(12923..13867)
                     /locus_tag="VFU75_04790"
     CDS             complement(12923..13867)
                     /locus_tag="VFU75_04790"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HEU5466183.1"
                     /translation="MTFSRSQLLLLAGTANRPLAEEMAQHLGQPLCAVTIRRFADGEI
                     FVKIDENVRGRDVFIIQPTNPPAENYIELLLLIDAARRASAARVTAVLPYFGYARQDR
                     KDQPRVAISAKLMANLISHAGADRVLGMDFHSHQVQGFFDLPVDHLYSAPVFVAHFRT
                     KDLRDPVVVAPDVGSAKMARGFAKRLNASLAIIDKRRPSANVAEVVNVVGDVDGKDCL
                     IPDDMIDTAGTITEAASALKRLGAKRVFAFATHALLSGPAVERLRAAPIEEVAVTNTI
                     RLTPERQFEKLKVLSVGAILAKAISYEHSNQSVSSLFD"
     gene            14008..14457
                     /locus_tag="VFU75_04795"
     CDS             14008..14457
                     /locus_tag="VFU75_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466184.1"
                     /translation="MRKMLVAVALLGGAVAPLAAQGGGGMGGGRGFAPPPNHWMTMDS
                     VGVALGLTAAEMTAIQPQYDSINAILKRSAAMRDSIRASMQSGGDPRAAMAAARPIMQ
                     SLQEQEDALIKEIHGKISAAAAAKLDAAPPPRVMRQRPGGMGGGPGR"
     gene            complement(14559..16886)
                     /locus_tag="VFU75_04800"
     CDS             complement(14559..16886)
                     /locus_tag="VFU75_04800"
                     /inference="COORDINATES: protein motif:HMM:NF018190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEU5466185.1"
                     /translation="MLPLLWLLAASHPPLRPTPPAAGPYSGTAGNVAVATPRIDASAN
                     IDGSLDDPPWRQAAVLTGFSEYAPVDGRPADDSTQVLVWYSATAMYFGIRAFESHGPV
                     HATLATRDHISTDDMVEILLDTFNDHRQAIALGVNPLGVQADGTLNETHAGTSDTVDL
                     STDFVYQSKGRVTDYGYEVEVRIPFKSIRYQPAATQTWGLQIIRTVQHSGHVQTWTAA
                     RRRAASFLGQSGTLEGLSNLHRDLVLDLNPELTGKVDGAPSGPGWSYHHLNPDVGGNV
                     RWGITNNLTLTGTVNPDFSQVEADASQLVFDPRQALYFPEKRPFFLEGLEQFATPNNL
                     VYTRALVQPVGAVKVTGKVGNVGIGVLSGLDAQAFSATGTDNPFYNILRARADLGGQS
                     TAGIVYTDKQDGSDWNRVMGADARITFGGIYTLGLQGAESFTRTAGATVQGPLWSASL
                     VRSGRQFAFSYSLAGIHPQFFDQSGFISRNGIAVGQADHQITFYGAPGQALESWSGDI
                     LLFGRWLYPHFQAGDAPDDREIHFNTNTVIRGWTVTTGLFFERFGYDPSLYANYALQR
                     TVGAVTDTVPFVGVPSIRNLDILLQFSTPQYRHFDANLLFLPAIQDENFYEWSPAQIL
                     LIQAGVNWRPNDRIRVGGTYLHQEYWRRSDGSLVARQLIPRLKVEYQVSRPLFVRVVG
                     QYVSTFQDSLRDDSRTNDPILIKNPATGVYERALQITDNGFRMDWLLAFTPSPGTVVY
                     AGYGSSLDDQGTFAFRGLRRTTDGFFAKFSYLFRF"
     gene            17014..18138
                     /locus_tag="VFU75_04805"
     CDS             17014..18138
                     /locus_tag="VFU75_04805"
                     /inference="COORDINATES: protein motif:HMM:NF019365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEU5466186.1"
                     /translation="MRSFVVVASGLVLLLGAPRRVLSGQASRPFTLGADISYLDAPAV
                     RGRAHRTYQENGKADDELSILMRHGWTSFRLRVFVSPVREAPDNSLANTIPLARRIKA
                     AGGTFLLDIHYSDTWADPQHQEIPVAWRDLSFDSLEARVESYSRDVIRTLKDSGAMPD
                     WVQVGNEITRGTLWPLGQVHVPGAAQYNPPSGSDSIAQWDHLIRILKAGIRGVRAGAG
                     NTPPRIAIHIDRGGDWVTTEWFFDHLEAAHVDYDIIAQSFYPPWRHGTLEDLWKNMTE
                     CARRYHKDFLVAETGYEPTHSPDNPDMLWPVTPEGRLQFMVDLVNTVKKAGGIGVMYW
                     APEWDLWNADGTPGPAVFTLDHLNDLRARPASHVPPEVAP"
     gene            complement(18162..19553)
                     /locus_tag="VFU75_04810"
     CDS             complement(18162..19553)
                     /locus_tag="VFU75_04810"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5466187.1"
                     /translation="MKRIAVSLLLFAAVTPLAAQTPVNWDSLGAETQRVLTSYLRLNT
                     TNPPGNEIQTARFLQEILRENGIESQILDTAELGAGRANLYARLRGNGSKKAIALVSH
                     MDVVPVTPAFWSVDPFGGVVKDGFLYGRGALDMKGEGIAELMAMLAIKRSGVTLNRDI
                     VFIGNADEELGGTGAETFVEKHADLLKDVELLITEGGSNPLDHGRLLYYGVSVSEKRT
                     FWQRMTVHGIPSHGSRPTKNNPVPHLVAALDRIAHYETPIRVTPGVRKYLHDVSSQFT
                     GDHATWLSDVDAALKIPAARDWILGNVEWNAILRNTISETGLSGSSKTNVIPAEATAD
                     LDIRLLPDQDTAAFRAELVKVVNDTAVHWSTILPPKAPLESPIETDLFRAVERAAHER
                     DPKALITTVMLTGATDRPTYRALGIVTYGLDPFKVELEDDQRGVHGNDERVSLENLRF
                     GPHFFYDILRYVQ"
     gene            19700..23020
                     /locus_tag="VFU75_04815"
     CDS             19700..23020
                     /locus_tag="VFU75_04815"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466188.1"
                     /translation="MRATLALLVLLPVSTVLAQGGRKKPAPKTAAATPGAPSPRADSG
                     GLGGIVGGLKLRSIGPALTEGRISDIAVDPTDKAVWYVATAAGGVWKTINDGTSYTPI
                     FDGEGSWSIGAITIDPNDHNVIWVGTGENNAQRVVAFGDGVYKSVDGGKSWKNVGLKE
                     SQHIGRIVVDPRNSDVVYVAAQGPLWSGGGDRGVFKTTDGGATWTKVLAGDDWTGAND
                     IQLDPRHPDVLVATTWQRERRVYGFIAGGPGSAVWRSTDGGKTWVKSQSGFPKGDLGR
                     IGLSRSPADPDVIYAIAQAADEKGGLFRSHDGGASWEKMSGTQFGGNYYNRVFADPKN
                     VDRVYAVNVFLQISNDGGKTFSSLGEINKHVDNHVVWIDPDDTDHLVVGCDGGVYETF
                     DHGKSWRFGANLPVTQYYRVATDNAEPFYNVYGGAQDNFSVGGPSRTRTNNGIRNSDW
                     FITAGGDGFGSVVDPTDPNTVYAQSQFGNLVRFDLRTGEQVAIQPFDETTGEALRWNW
                     DAPVIISPHDHHRLYFAAQRVYRSDDRGDSWRPVSPDLSRQIDRNALKMMGRVWGVDA
                     VEKNVSTTLFGTIFTLAESPLKEGLLFAGTDDGRIQISEDGGAHWRATDHFPGVPDTT
                     FVTRVVPSTRDVNTVYATFNGHWSGDFKPYVLKSTDLGRTWTNISGDLPERGDVWCLA
                     EDGVDPNLLFVGTEFGLYFTADGGQKWTRLQNGLPTIQVRDLTIQRRENDLVLATFGR
                     GFYVLDDYTPLRHLSSDNLAQRAVIFPVRPAPLYVPSSPLGLPGVGFQGAGHYSADNP
                     PYGAVFSYFLKDPILSRKERRQKAEAAKEKQGADIGYPSWDSLKAEDREEDPALIVTV
                     SDSAGNVVRRFTGSTSKGINRVAWDLRLPTSNPVDGPPFKADPNNPFGSGPRGPYVPP
                     GTYQVSLAERDDSVFTPLGTPQRFRVVDIDSALPGRAYASLSDQERVAALQREVLGAS
                     ALVNEALTRVGYLKRAIDETPTADATLASRARVMENRLRDARELLSGDPTLARHQEPS
                     PQSLLDRLRGAVGGRSGSSLAPMNAAERAQFDYVRTQFSVVMPKIQRLIDVELKSLED
                     AAEAAGVPWTPGRMPKAP"
     gene            complement(23023..23093)
                     /locus_tag="VFU75_04820"
     tRNA            complement(23023..23093)
                     /locus_tag="VFU75_04820"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23059..23061),aa:Gln,seq:ttg)
     gene            complement(23160..23552)
                     /locus_tag="VFU75_04825"
     CDS             complement(23160..23552)
                     /locus_tag="VFU75_04825"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEU5466189.1"
                     /translation="MAKKSSPPPGKTASWMIDRRIEELADWRGEMLARLRKLIKQADP
                     DVVEDWKWAVPVWKHDGMICTGETYKRAVKLTFPHGAALKDPSKLFNSSLEGGTRRAI
                     DFPEGAKINEKAFQALIKEAVTYNQNPD"
     gene            complement(23680..24378)
                     /locus_tag="VFU75_04830"
     CDS             complement(23680..24378)
                     /locus_tag="VFU75_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2071 domain-containing protein"
                     /protein_id="HEU5466190.1"
                     /translation="MPRPFLTAEWCHLVMLNYQVDPALLEPHLPAGTHVDSHDGHCYA
                     SVVAFQFLNTRVLGLPIPFHRDFPELNLRFYVRRTVAGEVRRGVVFIREVVPRRAIAL
                     IARLIYNEPYVSLPMRSSVRDGAVRHEWRRDGRWEGLTATFTDSAAIPRAGSHEEFIT
                     DHEWGYTRQRDGGTIEYRVQHDRWPVRQATDVRIDADFPGLYGPTLGTALANPVTAFV
                     VAGSPVTVGKPVRV"
BASE COUNT         4185 a         8004 c         8197 g         4085 t
ORIGIN      
        1 tgtgggagtg gcaactcccc aacaccacgg tcgccaggat gagacacgcg gggcgggtag
       61 acaaatggcc tcgccgacaa aataggaagt cggcgaggcc ggggcaagtg gttcagccga
      121 acggagttag aacgtcgggc cgagggacag ggtccagtat gcgtggctgt tgttcggacg
      181 gtcgagcggt ttcgtgtaat cgagccgcag gatcacgatg ccgaacatgt tggcgcgaat
      241 gccgaggccg taactccgca ggggaacgcg gacgttctcc gggctgcagt tcgggtcgct
      301 cggagtggcg caacgcgtcc aatgcagctg cgagacgctg ttgaacgcga cgccagcgtc
      361 gtagaagaac gcgccctcga gtggcgggaa gccgagtggc agcacgccga gtaccagggc
      421 acgcgtgagc gggaagcgga tctcgacgtt ggcgaccgcg acctgcgaac cgatcagctg
      481 atcgagctcg gcgcacccgg tttgcgagct gccgttcgtg accccgacgc attcgttgct
      541 ctggaacgag ccggcggtat atccgcgaat gagctccgta gacccgagga acgttggaaa
      601 ccgcccgcca tcccgaccca cgcggccgaa gtagagtcca cgcacagcca gagtgaacgg
      661 gcggaagagg aagtaccgcc gccagtcggc ggtcatcgcc gtgaactgcc aattgccgat
      721 ggcgggcgcc acttcgagac gccagcggtt accggcgaag gggccgacat agctgaacag
      781 cgtgttgtca tgcaccagcg cggcgctggg ctgcgcgtac gcattggtca cgccgcctat
      841 ggtgttcacg ttctccccga cgggacaaaa cgcaccactg cagacgggtt gatacacgtc
      901 ctggatctgc agcgtcgcgt ctgaaatggc gacggtgtgg agggagaact ccacccggct
      961 gaactgactg aacggatacg aggcggtgcc gaacaagtcc cggatgacaa atcgacggat
     1021 gttggtattc aagacatagg tcggcggtac cgcggccgag tcgaacgagc tcgtggacgg
     1081 ctcgtagaag tagtacggct cctgggtgaa cccgacggcc cagttggtcc ggtgtacctg
     1141 attgatgtag gcggccagga cctccgcttc ggccagccgg ccgttcacgg cgccggagaa
     1201 gaccatggtc cggtccccca gcatgtcgga gagcgacacc gccgtaccgc cgaagaagcc
     1261 ccgtccgaaa ttgtcgcgtt catagccgat cgtcggccgc gcgacgtagt ccggcgtgaa
     1321 gcgaacgtgg tacggcatga tcgagaattc agcggtgtcc ggcagcgcga gcgtattcga
     1381 gtcgaggagc tgcttgactg acacgggggc cgaaaccttc gtggtctccg cgggctgagc
     1441 gcccgactgg cggagccccg acggagcccg atagatcgag ctcgacggga cggaggaggc
     1501 gcccgtgtcc gagggactgg ccggcgtgat cttcccggtg tccgcgttca ggctggccat
     1561 ttcgtaccgc ggccgggtct gcgcatacac caggggcggc atgggcttgt cggcgtacgg
     1621 ccggccacgc agcgagcgcg gattgtcgag ggcgtagacg tcccagctgc cgtcgtcgta
     1681 ataggcgaac gccaggcggt cggtctcctt ggcccaggag agggcggggg agatcggcgt
     1741 gatgcccgaa acaccggtgt agacgtcggt cagctgatag acctgcttgt cgcccaggtc
     1801 gtacaggaaa atgttcggaa taccggtgcg gtcggagacg aacgccagcg agcggccgtc
     1861 aggagcccag acgggattca cgttccgccc gtattccatg tgatcgagca cttcgatctg
     1921 accggagtcc aggtgataca aggcgatccg gaagttgccg gtggagagcg tctggaagtt
     1981 ggtgttcggc ccacgatcgg tcgaaaaggc gatcgtcttg ccgtcgggag accactgggg
     2041 caggaggtcg gcgtacttgt cgttcgtcag ccgccggagg tccgagccgt cccggttgat
     2101 gatgaagagg tccgccagcc ccccgtcgta cccggtgaac gcgagctggg tgccgtccgg
     2161 ggaccaggtc ggattgttga ttccgttcag cgggatcttg aggcgcgcgg cttccttgtt
     2221 cttcttgatg tcgaagagga ccaggtcgtc ccggtccttg tgcttcacgg cgatggcgaa
     2281 gtaccggccg tcgggtgaga atgagccgga cgaatagatg aagcgcaggc tctcgtagtt
     2341 gctcgacgtc gtggacttca ccagccgccg gatggttttc ccggtttcgg cgtccgccag
     2401 atacaagtcg atgaagaagt cgttcccctc accgaagtag gcgatgttct tgccgtcggg
     2461 cgagagggcc ggagccagat gcagccgccc gtccgagcgt ttctccgtga gcagcggctc
     2521 ggcgatgcgc cgcgcgggat agtgactcga gatctcgggc aggaacgtcg cctggacggc
     2581 atcgcgccag tccttcgaca gctcgtccag cgagatcccc agggcccgct tgaaggcgcc
     2641 ctcgatcccc gcgccccgcg acgcctgcag gatttctccg atgacctcgt caccccaacg
     2701 ctgtccgatg tacgcgaaga gcgcgtgccc gtagaagtag gggaagatgt tgggattgtc
     2761 cgtcatgtct tcgatggtgg gcaggtggcc ctggagggcc gcgtcccgca acgccatcgc
     2821 cgtcgccgga tccagcggac cgagcgacag atactccgac atcccctcca tgaaccagcc
     2881 cggcgggttc acggcgacca tggtctgaat gccggcgccc gggtggcccc ccgagtacac
     2941 gtcgtactgg aactggtgcg tcatctcgtg ctggatgacg tgctccatcc cttcgtagtc
     3001 cccggtgaac gggatcacca tccggtgctt gaagaactcc gtgactccct cggtgccctc
     3061 gtcgggactc tccccgccca gcgcgttggt ctcctggaac tccgagtacg acgcgtacag
     3121 gagaatgggt ttgcgttcga ggaagttgtg atggaggatg cgtgagaggc gcgcgtacga
     3181 ccgttcagcg atgcgggcca cgtcgagcgc ggcttcgcgc tcgacgggat agaagtagac
     3241 gtcgaagtgc tcggtctgga tgatctgcca gttgaaggat cggtactgga ccttgttctg
     3301 tccgaagtac tgcgccgacg ccgtggtgct gagcgcgacg cacgcgagcc ccgtcaagaa
     3361 aaccttcgtc cccttcatac cacccccggt cgagccttca ttcacccact gcgaccaccg
     3421 gtgcttcacc ccagagccgc tccaactgat aaaaatcgcg gagctcggga tggaagagat
     3481 gcacgacgaa gtccacgtaa tcgagcaaca cccaccggcc ctgcgtcagc ccttccacat
     3541 ggtggggttt gttctcccca ccgagcgaca tcaaatgatc cgccatgccc cgcacgtgag
     3601 cgtccgaggt ccccgacgcg atcacgaagt agtccgtcgc ttccgacaat ccccggaggt
     3661 ccagtaccac cacctgcacc gctttccggt ccgacaaccc ctccaggatg ggccggagcc
     3721 cctccagggc gggggccggt ttcccgcggg aacgccctcg accgctactc atagcgccgc
     3781 ccgccgtcgt ttgttccgtc ctccacctga taaaagacgc cccaggtacc gatcccggtg
     3841 tgggccgcga gcaccggcgt gatcggactg atcaaagctt ccttgggcgt gtagcggcgg
     3901 accagttcct gacgcaaagt ctcggcgaac tggtggatgc cggcgtgtac cacgccgagc
     3961 cggagcgact ggggcctggc ggcgagcgcc cggtcgagga gggcgagcac cttcgtccgc
     4021 gcggcctccc gcccgcgcgc acgccccgcg gaatcgacct cgccttccgg cgtcagcgtc
     4081 aggatgggtt tcaggttcag gcgcgtcccg agccaaccgg ccaggcgcga cacccgcccc
     4141 gagcggatca ggcgatcgag cttgtcgacg acgaagaagc cggatgattg cgtccggacc
     4201 cgcgcgagct cggtcgcgat ctcccccggc ggcactccct gtgccgccag ctcgaggccc
     4261 cggaggacca gcatgccctc cccgagggac gcgctgcggg agtccacgag ggagatccgg
     4321 tcggacgcca tttctttcgc ggcggcctcc gcattcgcgt aggtccccga caacggcttg
     4381 gaaacagtca ccaccacgat cttctcggcc acccgcaggg cgtcctggta cacctcggtg
     4441 aacgcctggc gggtgggctg ggaggtggtc gcatcctggc ccgaccggag ccgcgtaaag
     4501 aactccgggg cggagagctc gagccggtcg cggtagacgc ggtcgcccac gacgacctgc
     4561 atgggaacca ggccgacctg cttctcgaac acgacggaat cggggagatc gcatgcggtg
     4621 tcggcgacga aggcgacggc gcgcttgttc acgagggcct gatgctgggc ccgcatgtcc
     4681 tccgccttgg tgtactcgag agtgccccag ccgcggccga gctcgaagac ccggtcgggg
     4741 gtgtcggtgt ggacgtgggc cttgaggagg tcgcccgatt ccaggacgac gatggacccc
     4801 ccgagctccc ggagcgccgc gcggacctgc tccggcttgg gcagggaggc accccgcacc
     4861 atgacttccg tgcagaaccg gtagtcgcgg tcggaggcta cctcggtggt cgcggcggca
     4921 tttacgaggt cccggtcgac ggccccctgc gcctggcccc cttcttctat aaggcgcttc
     4981 acgccgtcca gcaggcggac gaagcccttg gctccggcgt ccacgactcc cgccgacttc
     5041 agggcctgga gcagctcggg tgttctctgc agggagagct cggcgcgggt caccagacgg
     5101 cgcatgaaca ccttgaggtc ccgctcatcg gcggcggcgt ccgcttcgtc ggcggcgtcg
     5161 cgggcgacgg tgaggatggt cccttcgacg gggtcgtcga gcgcgccgtt caggcgttcg
     5221 aagcccgtgc ggatggcgcg ggccaggtct cgggggctgg cggtggggcg gtcccccaga
     5281 ccatcgcgga gaccgaggag aaactgcgac agcatcatgc cggagttgcc gcgggcgccg
     5341 cggatgctcg cctgcgccat cgtctccgtg acctggggca ggggggcgtc gccgagggtg
     5401 cggagggcct gcgcgacttc gcggagggtg agccacatgt tggtcccggt gtcgccgtcg
     5461 ggcaccggga acacgttcat ccgattcagc tcatcccgac ccgccgcgac ccagtccgcc
     5521 gcggccagga gcgaccgcga cagtcggggt ccatcgacgt aactgatccc ggcgggcacg
     5581 gggcgcgcct tactccttga ccaccgtcac ctgcacttcg ggccggacgt cggcgtgaac
     5641 cttcacgccc actttgaact cgccgagcgc gcgaatcggc tcgggaagat cgatctggcg
     5701 cttgtcgacg tcgtggcccg cttccgccag gcggcgctgg atgtccgcgg cggtgatgga
     5761 cccgtacagc ttgtcttcct cgccgacctt caccgagaag gtgagctgga cctgcgcgag
     5821 cttgtcggcg atgacctgcg ccgcatcctt ctccgccgcg cgcaccttca tgatgcgtgc
     5881 cgcttcgaag gtgatcttct tcttgttgcc ctcggtggcc ggataggcca ggcctttggg
     5941 cagcaggaaa ttgcgggcga agccgtcctt gaccttcacg acctcgccgg ccttgccgac
     6001 gtgctgcacg tcttcccgca gaatgatttc catgccgtcc tcgtgatcag ttcggccggc
     6061 cctcgagccg gcgccggaac tccaaccagg tatcactcac acccaggacg cagaggcttg
     6121 gcgcggtggc gggagcgaag agcactcccg aaagcaccgc cagcgcctgc acccaggccg
     6181 gaaccgcgac catggcggcg gcggccgcga ccacggccgc cccgcggagc acgtacaagg
     6241 ctcccagaat cagtccgaca ttcaccgcgg cgacgcggat gcccggggcg ccgcccgaca
     6301 gaatccacgc gatcgtcgcc gccaccacac cccagaccgc gccgtccgcg agccggaact
     6361 cccggaacgg ccgcagcgcc gggcccagcg ggtgccgagc ggtgcgcacg tgccattgcc
     6421 acgccagcgc cagggccgcc agtccccaca ggatctcgag cgccggacgc accgtactga
     6481 aaaggcgcac cgccggctcg tagttcgcat acatgtccgc cacgacgtcg gaggacatga
     6541 cttccagcac ggtccgcagg gcgaagctgg tctggcgcac cgtatcccag gccaggaggc
     6601 cccaggtgtt cgcgccgttc agaatccacg cgagcaccac cgcgctggcg ctgctccacg
     6661 ccagcgcacc ggccgcgcgc ggcagaaacg gtccgggcgc gttccacgcc atcaccacga
     6721 acgccacggc ggcgaagagc tgaaagacac cgatcaccgc gtcgaccgcc gtttcgcgcg
     6781 gcaggaccag ggcccagagg gcgagcacgc ccatcaagag cgcgcccgcg cggaacacac
     6841 tggaccgcgt gcctccgatc agctggaggc ccgcgaacgt gagccccgcg agcggcaggc
     6901 tggtcggcgc gaagagggcg agaagcaggg tgcccgtcat cagcgaccag aagccccgcg
     6961 ggaacaccat tgtcagccgg tgaagccctt gatgtacggg atcagagcca gctgccgcgc
     7021 gcgcttgatg gccgtggcca gctgccgctg gtgccgcgcg cacatgcccg acaggcgcga
     7081 aggcagaatc ttcccgcgct cggtcaggaa acggctcacg gtccggtcgt ccttgtagtc
     7141 cagaacccgg acgcccgact cacacaccgg gcacgtcttc gaagggcgtc tcacgcgtcg
     7201 tcctcctcac cgtcatcgtc gccgccgatc accggcgcag gcacgggctt ggcctccgct
     7261 tcggccgcca ccaccaggaa acgcaggacg ccctcgtcca gcttgaccgc gcgctcatac
     7321 tcggggagca ggtccccggt ggtctcgaac tgagcgacga cgtagtagcc ggtgtccttc
     7381 ttcttgatgg gataggcgag ggtccgcttg ccccaatggc tcacgttcgt catcgagccc
     7441 ttgccgtctt tggtcaggag ggcgtgaata cgatcgagct tctcggtgac tgcgggttcc
     7501 tcgagggcac tgtcaaagat gtagaccgtc tcgtatcgac gagtcatgcc acctcccttt
     7561 ggtcttgaac gacccggtgc aggtgcggca ccgagtaggg gaattgagcg gagaaagcta
     7621 gtgggatggg ggaggggggt aaaggtgggg aacggcccgg ccctcgtggg tatcctacag
     7681 gagccatgtg gcacttcgcc gccctcgccc tgagcctcct gaccgccgat tccacggtgc
     7741 tcaaggggac ggtcctctca ctgggcagcg gtcaccccct cgccggggtc accgtgagcc
     7801 gccccggacg cgcgcccgtc accaccgaca gcgccggcca cttcgccatc ccggggcccg
     7861 ccgcgcgtct ccacttgacc tggaatggcg tgagcggcac cggggtcatc ccagccgggg
     7921 cggaagacca gtcgttgttg gtggtgatcg atacgggagc tgccgatctg agtcccacca
     7981 tcgagcacga ggactggcat ctcgccggcc actggggaat gccgggattc ttcggccggg
     8041 cgcgccaggg atatgggacc ttcttcaccc gggacgctct ggtccgctcc ggctttacga
     8101 acctcaagga tctgctcagc tcgctcggga tccatcacgg ctgcctccgc gacggcggcg
     8161 gctgcggagc cgtgatctac tatcggaacg tgccgacgct ggtgagcatc tacgtggacg
     8221 gggcgtttca gaacaccgag caggcagatg accgctcact cggtgacgtc acggggatcg
     8281 agtactaccc gctgccgaat ccgccgaggc ccgcgctcgc gagtcctcgg gagtggatcg
     8341 tgaagaccga ttacctggag cagctaccgc ttcaggagtt caccgtggtc ctctggacgc
     8401 gagacttcta ccagcggctg gcggaacgct gagcacacct tgatgtgcag cgagacgtct
     8461 cgcgagcgtc tcactggcat gtctcacggc cgggtcatcg caatgctatt gctcgtcggc
     8521 gcctgccatt ccgcgccggc cgcaccaccg cccgttccct acgttcccgt cgagttgcgc
     8581 gtcacgctgg atcgatccgc ctacgcggag ggggatccgg tctacgccct ggtggagctc
     8641 aggaacgtgg gcacggacac cgtgtgcgtg ccgtcgatga gcctggatca ggattggctc
     8701 aagatggacc tgtctcgcga cggtgttcgg atagcgggcg ggaagatgct ttgggtggat
     8761 gagttctaca gccccgggta ccttggcgag ccgttgccgc ccgggggagc gcactacgaa
     8821 ccgatcctgc tttcggagcg ctggtcggat ggggatcgtg tgtcccaggc gctgtttctg
     8881 ggacgactcc ccccgggcct ctacgcgctg gtcgcacagt tcaacccgcg gatccattgg
     8941 tggcctggcg ctaccgttac gtctatgccg gtcgcgttca tcgtccgctc gcgtgactcg
     9001 gcggagaacg cgcgatacga tctctggatg cggttcgcca agcagcttca accgggacac
     9061 tggccggccg aggtgacgga ttccctcatg ggggccctgg cggcgcgggt ggcgggggac
     9121 agtgccgatc cgctttcggc gacgctcgcg gagttcgtgg ccacggggga gatggttgcg
     9181 cagggacggc gcgaccacat cgcgatcatc cgggaatggc agagcgccgt catgcggcat
     9241 gagcccgatg cacccgctgc cgcctgggcc gccgcgcgat acggcgagtg gtggctgcac
     9301 ggcgacacgg tgccgattcg ccggctggcg gagtccctgc acgggacggt ggcgggtggc
     9361 atcttggatc ggagcctcgc tcgccattcc tcccctccga ctcgctgtgc tccccgaact
     9421 cctatacgtt gaaccggaag agcaggacat ctccatcctg caccacgtac tcccggccct
     9481 cactgcggac caggccctgc tcccgggccg gtttccagcc tcccgccgtg gtgaaatccg
     9541 caaagctcac cgtctcggcc cggatgaatc ccttctggaa gtccgaatga atgacgccgg
     9601 cggcatcata ggccgtgtca ttcacgtgaa tggtccacgc ccgcacttcc tgttcaccgg
     9661 cggtgaagta ggtccgaagg ccgagcaggg cgtacccggc gtgaatcagg cggtcgagcc
     9721 cgggctcctt caccccggcc cccgcgagga actcctgccg ctcgtcacgt gccagctccg
     9781 ccagctccgc ctccagcttg gcggagaagg gtaccagctc ggcttcctcg tgatgcgcgg
     9841 cgacggcctt cttgagcacg tcgacgaact tcccgccacc ggccgccaga tcgtcttcac
     9901 tcacgttggc cgcgtacaga atcggcttgg aggtcaacag ccccatcgcc cgaagggccg
     9961 cgagctccat ggcgtccttg gcggcggccc gcgcgccctt gccccgatcg agctcggtgg
    10021 tgacgcgctc caggagagcc atctcgacct gggcgtcctt gtcccccgac ttcgcgctct
    10081 tgcgggtctt gtccatccgg cggtcgatga ccgacacgtc agcgagggcg agctctgcgg
    10141 agatcacgtc atggtcccgc accgggtcga ccggacccat gacgtggacc acgtcgggat
    10201 cttcgaagca gcggatgacg tggacgatgg cgtccacttc gcggatgtgg gacaggaact
    10261 ggttgccgag cccctcgcct agcgacgcac ccttcacgag gcccgcaatg tccacgaatt
    10321 ccacgacggc ggggaccttc ttcttcggct tcaccagctc cacgagctga tcgagacgag
    10381 ggtccggaac ttccaccacg ccgacgttcg ggtcgacggt gcagaacgga taattctcgg
    10441 cagccgcggc tttcgacgaa gtcagcgcgt tgaagagggt cgatttcccg acgtttggca
    10501 gaccaactat cccaaggcga agcatctcat tccttctatc gcagaaatgg agcgatcacg
    10561 atccaggtga ggaccgccac ccgcaccagc gcgaagcggg ggccctttcc ggcggcgccg
    10621 atcacgccga ccaccaccgc ggccacgaac gtgtgaaacc agccggacca atggctcgga
    10681 gtcaccagag gactgccgga gtgcgaggcc accaccaacg cgagggcggc gatgacggtg
    10741 acggcctgag cgggcgcgga cggcagcgtc accgcggtga cggctgcgag ggcggccacg
    10801 gcggctccgt acggacccac gacgtgaacg gacgcgacgg cgaccacgac gaggccgatc
    10861 gtgaccatcc gtcgattgat cagcgtgatg cctgctccca ggacgacgcc tccgagcccc
    10921 gccagcagcg tgacttgggg ggacaacgtg tcctcatgga gtcccaacgc gaccgggtcc
    10981 agtcccgtgg ccgcgagggc cacgaggcac ccgaccacgg cggctcccgt aaccagcccg
    11041 gtacgcgcag accctttcca caacagggcg accgctcccc cgaatatgcc cgtagtggcc
    11101 acgagcatcg ggtacgccac tccatccagt cgattcgccg caaagggtgt ggctgccgcg
    11161 accaccaggg cagctgccgt tgcgaagacg ccggcgtcgg aggcctccgg gaccatgctg
    11221 tcgcttttcc ggctcgcgga aatcaccgac gaaccgaggg cgaaggccgc gatgagctgg
    11281 gcgaagagcc tccagccgcc atcgtccatc cccgcggccg agccgaagag ctcgaatacg
    11341 agacccagac cgaggagcga cgtgccccag gccgacagac ccagggtccg ggattcgatg
    11401 ccgaaatccg ccatccattg aacgctgagc gcggcgatgg cgccgagggc gaaactggcg
    11461 ccggtgcggg cgctgatcag tccgtcgagc accacgagcg cgacgaccgc gaccaggccc
    11521 ggctccgcaa acgtgcccag ttccggacgt cggttggcgg cacgcagggc gacgagggcg
    11581 gggatcaggg ccagcgtgcc cgccaggatg gcgatctcgg ggaaccaggg caccaggttc
    11641 atgcgggcgg ctgtccctga agccagcgct ccacggcctc gaccatctgc ggcatcgctg
    11701 cttcgatcag gtcccgctgc tccgggtccg ccggcgcgag gacgtagtcg gcccagtcac
    11761 cgacgccttc gggcagggga ccgacgccga tgcgcagccg cgcataggct tgcgattgca
    11821 gggcgccttc gatgctcttc agtccgttgt ggccccccgc cgatccgtcg agccgcaggc
    11881 gaaacgtgcc ggcggcgatg cggaactcgt cgacgagcac gagcaggtcg gtggcgggat
    11941 tgaacgtcgg atcggcgcgg agcgaaacga gggccgcccc cgaccggttc atgtaggtct
    12001 gcggcttgag gatgcgtacc ggcacgcctg ccacttcacc gtcggaaacg cgagcgcgct
    12061 caccacgccg gaaggggccg aacccccacc gcgcagtgag cgcgtccgcc agccagaacc
    12121 ctgcgttgtg gcgcgtcgcc tgatactctt cgcccggatt ccccaggccg acgacggcgc
    12181 gcagacctta tcccttgggc ttggcttcgc ccttgggctt ggcctcgccc ttggcctcgg
    12241 ccgcaggctt gccctcttcg ccttccactt cctcgtcctc gcgctccttg cggatgagct
    12301 cgggctcggc gacgtcggtg gccccttccg ccgccggcgc cacgacttcc tcggcacggg
    12361 gcggcacgac taccgcgatg gtgagatcgg cgagagtcag gatcgttgcg ttcgggatga
    12421 ccagatcccg cacatggagc gagtgcccga tgatcagggc cgacacgtcg agctccacgc
    12481 gatccgggat gtgctcgggg agcacttcga tctccacttc gcgcgtcacc tggtccagca
    12541 cgccgccggc attgcgcacg ccgtcggggg tgccgacgag gtgcaccggc accttgagct
    12601 tgaccttctc atccgcgtgg atctcgtaaa agtccacgtg gatgatgtcg gggcggacgg
    12661 ggtgccgctg gatctcgcgg atgagcgcct tcgccttctt gccgtcgatg gtgagctcga
    12721 tcagcgtgct ctccggctcg atgcccgtga gcgccttttc gagcgcgggg gcgtcgacgg
    12781 tgaggttctg cgcggcccgg ccatgtccgt acaccacggc cggcaccttc ttggcgaagc
    12841 gactctgacg ggccgcgcct ttgccggtct ggtcccggtt ctgcgcggtg agactgacga
    12901 tctgtgccat accactcctg ccttagtcga aaagagaact gaccgactga ttcgaatgtt
    12961 cgtagctgat cgccttggcc aggatcgccc cgacggacag caccttgagc ttctcgaact
    13021 gccgctccgg ggtcagccgg atggtgttcg tcacggccac ttcttcgatc ggcgcggcgc
    13081 ggagccgctc caccgccgga ccggagagca gggcgtgggt cgcgaacgcg aacacgcgct
    13141 tggctccgag ccgcttcaac gccgacgcgg cttccgtaat ggtacccgcg gtatcgatca
    13201 tgtcgtcggg gatgagacag tccttcccgt ccacgtcacc gaccacgttc accacttccg
    13261 ccacgttcgc cgacggtcgc cgcttgtcga tgatcgcgag tgacgcattg agccgcttcg
    13321 cgaatccgcg cgccatcttc gccgaaccga catcgggcgc cacgacgacg gggtcgcgca
    13381 aatccttggt gcggaagtgc gcgacgaata ccggcgccga gtacaggtga tctaccggga
    13441 ggtcgaagaa gccttgcacc tggtgggaat ggaaatccat gcccaacacc cggtccgcgc
    13501 ccgcgtggct gatcaggttc gccatcagct tggcgctgat cgctacccgc ggctgatctt
    13561 tccggtcctg ccgcgcatat ccgaagtagg gcaggaccgc cgtcacccgg gccgcactgg
    13621 cccgccgagc cgcgtcgatc aggagcagca gctcgatgta attctcggcc ggcggattgg
    13681 tcggctggat gatgaagaca tcccgcccgc ggacgttctc gtcgatcttg acgaagattt
    13741 caccgtccgc gaaccgccgg atggtgaccg cgcacaacgg ctgtcccaaa tgctgagcca
    13801 tctcctcggc gagcggccgg ttggccgtac ccgcgagcag aagcagctgg cttcgggaga
    13861 aggtcaaatc cgccattttc tgagccgcgc aagatagccg ccaagcatcg caaagccaag
    13921 gggttaaaac gcctcaacca agcgggtccc tgcggcgtac agcaaccggt ggccccaatt
    13981 ccggggcctc cagggaggaa caaccgaatg cgcaagatgc tcgtagccgt cgctctcttg
    14041 gggggcgctg tcgcgccgct cgcggcgcag ggtggtgggg ggatgggtgg tggccgtggg
    14101 ttcgcgccgc ccccgaatca ttggatgacg atggactccg tgggcgtggc cctcggcctg
    14161 accgccgccg agatgacggc gattcagccc cagtatgact cgatcaacgc gatcctgaaa
    14221 cggtccgccg cgatgcggga ttccatccgc gccagcatgc agagtggcgg ggatccacgg
    14281 gccgcgatgg cggctgcacg tccgatcatg cagtcactgc aggaacagga agacgcgctg
    14341 atcaaggaga ttcacgggaa gatctcggcg gcggctgccg ccaagctgga cgccgcgcct
    14401 cctccgcggg taatgcggca gcgccccgga ggcatggggg gcggtccggg gcggtaaccc
    14461 ttgaaaaaac gtcaaggaaa agcgtcgagg taaatcgtcg aggggagccg tcaacgtctc
    14521 tcctcgacgt ttttccttga cgccttacct cgacgtcttt agaacctgaa cagatagctg
    14581 aacttcgcga agaacccgtc cgtcgtgcgc cgcaatccac ggaacgcaaa cgtcccctga
    14641 tcgtcgaggc tcgagccgta ccccgcatag accacggtgc cgggggacgg cgtgaacgcc
    14701 agcaaccagt ccatcctgaa tccgttgtcc gtgatctgca gcgcccgctc gtacacgccc
    14761 gtcgccggat tcttgatgag aatcgggtcg ttggtccggg aatcgtcgcg gagagagtcc
    14821 tggaacgtcg agacgtactg tccgacgacg cgcacgaaga gcgggcgcga gacctggtac
    14881 tcgaccttga gtctcggaat cagctgacgc gcgaccagcg agccgtccga ccgccgccag
    14941 tattcctgat ggagatacgt cccgcccacc cggatccggt cgttgggccg ccagttcaca
    15001 ccggcctgga tcaggaggat ctgagcgggg gaccactcgt agaagttctc gtcctgaatc
    15061 gccgggagga acagcaggtt cgcatcaaag tgccggtact gtggcgtgga gaactggagc
    15121 aggatgtcca ggttgcgaat cgacggcacg cccacgaacg gcacagtgtc ggtgaccgca
    15181 ccgacggtcc gctgcagcgc gtagttggcg tagagtgacg ggtcatagcc gaagcgctcg
    15241 aagaacaacc cggtggtgac cgtccagccc cggatcaccg tgttggtgtt gaagtgaatc
    15301 tcgcgatcgt ccggcgcgtc acctgcctgg aagtgcggat agagccagcg gccgaagagg
    15361 agaatgtctc cactccagct ctccaaggcc tgccccggcg cgccgtagaa ggtgatctgg
    15421 tgatcggcct ggccgacggc gatgccgttc cgcgagatga agcctgactg gtcgaagaac
    15481 tggggatgaa tccccgcgag ggaatacgag aacgcgaatt gacggccgga gcggaccagg
    15541 gacgccgacc acagaggacc ctggaccgtc gctcccgcgg tgcgggtgaa gctctcggcg
    15601 ccctgcaggc cgagggtgta gatgcccccg aaggtgatgc gcgcgtcggc acccatcacc
    15661 cgattccagt cgctgccatc ctgcttgtcc gtatagacga ttcccgccgt cgactgtccg
    15721 cccaggtcgg cgcgggcgcg caggatattg tagaaggggt tgtccgtccc ggtggcggag
    15781 aacgcctgcg catccaggcc cgaaagcacg ccgatgccga cgttgccgac ttttcccgtg
    15841 actttcacgg cgcccaccgg ctgcaccagt gcccgcgtat agacgaggtt gttgggcgtc
    15901 gcgaactgct cgagcccctc caaaaagaat ggccgcttct ccgggaagta gagggcctgc
    15961 cgcgggtcga atacgagctg gctggcgtcc gcctccacct gcgaaaagtc cggattcacg
    16021 gtgcccgtca aggtcaggtt gttcgtgatg ccccagcgta cgttcccgcc gacgtcgggg
    16081 ttgagatggt ggtacgacca gcccggtccc gagggcgcgc cgtccacctt gccggtgagc
    16141 tcaggattga gatcgagtac gaggtcgcga tggaggttgc tgagcccctc gagcgtcccg
    16201 gattgcccga ggaaggaggc cgcccgccgc cgcgcggccg tccacgtctg gacgtgaccc
    16261 gaatgctgca cggtgcggat gatctggagg ccccaggtct gggtcgcggc cggctgatac
    16321 cggatgctct tgaaggggat ccggacttcc acttcatagc cgtagtccgt cacccggccc
    16381 ttggactgat agacgaagtc cgtcgacaaa tcgaccgtgt cactcgtacc ggcgtgggtc
    16441 tcgttcagcg tgccgtccgc ctggacaccc agaggattca ctcccaaggc aatcgcctgg
    16501 cggtgatcgt tgaatgtgtc gagcaggatc tcgaccatgt cgtcggtcga gatgtggtcc
    16561 cgggtggcca gggtcgcatg caccggacca tgggattcaa aggcgcggat cccgaagtac
    16621 atggcggtcg ccgaatacca caccagcacc tgcgtcgagt cgtcggccgg gcggccgtcg
    16681 acgggtgcgt actccgagaa gcccgtgagg actgctgctt gccgccaggg cgggtcatcc
    16741 aacgagccgt cgatgttggc ggaggcgtcg atgcgcggcg tcgcgacggc gacgttgccg
    16801 gccgtgccgg agtaggggcc ggcggcgggc ggcgtcggac ggagtggcgg atggcttgca
    16861 gccaggagcc agagcagggg gagcatcgcg agacgactcc tacgggccgg gaagacgccg
    16921 cctacgcaac tccgcagcca caggtttcgc agttcaacgc acggacggtg ccatttggcg
    16981 ctacgatata gaatacgcgc ttcggagcct tccatgcggt cattcgtcgt tgttgccagc
    17041 ggcctcgttc ttctcctggg agccccccgg cgggtccttt cggggcaggc gtccaggccc
    17101 ttcacccttg gtgccgatat ctcgtacctc gacgcaccgg ccgtccgcgg gcgcgcgcac
    17161 cggacctacc aggagaatgg gaaagccgat gatgagcttt ccatcctgat gcgtcacggg
    17221 tggacgtcgt tccgcttgcg ggtcttcgtg tcgccagtcc gggaggcgcc cgacaacagc
    17281 ctggccaaca ccatcccgct cgcccgccgg atcaaggcgg cggggggcac gttcctgctg
    17341 gacatccact attccgacac gtgggccgat ccccagcacc aggagatccc ggtcgcctgg
    17401 cgcgacctgt cattcgattc cctggaggca cgcgtcgagt cctacagccg ggatgtgatc
    17461 aggacgttga aggactcggg cgccatgccc gactgggtcc aggtgggaaa cgagatcaca
    17521 cggggcacgc tctggccgct gggtcaggtc cacgtgcctg gggctgccca atacaacccg
    17581 ccctccggct ccgacagcat cgcgcagtgg gatcacctca tcaggattct gaaagccggg
    17641 atccgcgggg tgcgtgccgg ggcggggaac acgcctcccc ggatcgccat tcacatcgac
    17701 cggggcgggg actgggtgac gaccgaatgg ttcttcgacc acctcgaagc ggcccacgtc
    17761 gactacgaca tcattgccca gagtttctac ccgccctggc ggcatggcac cctggaagat
    17821 ctctggaaga acatgacgga gtgcgcccgg cgctaccaca aggattttct cgtcgcggaa
    17881 accggctacg agccgacgca ttcgccggac aatcccgaca tgctgtggcc cgtgaccccc
    17941 gaaggacggc tccagttcat ggtcgacctg gtcaacaccg tgaagaaggc gggcggcatc
    18001 ggcgtgatgt actgggctcc cgaatgggac ctctggaatg cggatggcac tcccggtccg
    18061 gccgtcttca cactcgacca tctcaacgac ctgcgcgccc gacccgcgag ccatgttcct
    18121 ccggaggttg ccccctgacc cctgacccct gacccctgcc gttactgcac gtaccggagg
    18181 atgtcgtaga agaagtgggg cccgaaccgg aggttctcga ggctcacgcg ctcgtcgttc
    18241 ccgtgaactc cccgctgatc gtcctccaat tcgaccttga agggatcgag tccgtacgtg
    18301 acgatgccga gggcgcggta cgtcgggcga tcggtggcac cggtgagcat caccgtcgtg
    18361 atcagggcct ttgggtcccg ctcatgggcg gcccgctcga cggcccggaa caggtcggtc
    18421 tcgatgggac tctcgagggg cgccttgggg ggcagaatcg tgctccagtg gacggccgtg
    18481 tcattgacca ccttcaccag ctcggcgcga aaagcggcgg tgtcctggtc ggggaggagc
    18541 cggatgtcga ggtcggcggt ggcttccgcg ggaatgacgt tggtcttgct cgatccggag
    18601 aggcccgtct ccgagatggt gttgcgaagg atggcattcc attcgacgtt gccgaggatc
    18661 cagtcgcggg cggcgggaat cttcagggcg gcatcgacgt cgctcagcca ggtggcgtga
    18721 tcgccggtga actggctgga gacgtcgtgc aggtacttcc gcactcccgg ggtcacgcga
    18781 atgggcgtct cgtagtgcgc gatgcggtcg agcgccgcca cgagatgcgg gaccgggttg
    18841 ttcttcgtcg gacgcgagcc gtgggacgga atgccatgca ccgtcatccg ctgccagaac
    18901 gtgcgtttct cactcacgct gacgccgtaa taaaggaggc ggccatggtc gaggggattc
    18961 gagccgccct ccgtgatgag gagttcgacg tccttgagca gatcagcgtg cttctcaacg
    19021 aacgtttcgg cgccggtacc acccagctcc tcgtcggcgt tcccgatgaa cacgatgtcg
    19081 cgattgaggg tcacccctga ccgcttgatg gcgagcatgg ccatcagctc ggcaatcccc
    19141 tcgcccttca tgtccagagc gccacggccg tacaggaacc catccttcac cacgcccccg
    19201 aagggatcca ccgaccagaa ggccggggtc accggcacga cgtccatgtg gctcacgagc
    19261 gcgatggctt ttttcgatcc gttccccctc aggcgggcgt agaggttcgc gcgccccgcg
    19321 ccgagctcgg cggtgtcgag aatttgagat tcgatgccgt tttccctgag gatctcctgg
    19381 aggaaccgtg cggtctggat ctcgttcccc gggggattcg tggtgttgag gcgcaggtag
    19441 gaggtcaaca ctcgttgcgt ctcggcgccc agggaatccc agttgacggg tgtctgggcg
    19501 gcgagggggg tgacggcggc gaacaggagc aggctgacgg cgatgcgctt catggggaaa
    19561 cccgggttcg agagtgggga taggctctgg ccggcgggcc tcagtgtcaa gaagggacgg
    19621 tgaaagggat gtgtgaatgc tcttaccgcc tgcctcctgc ggtcgtacaa tagcgcctca
    19681 ctctcgcccc ttcggaatca tgcgcgcgac actcgcactt ctcgtcctcc ttccggtttc
    19741 caccgttctc gctcagggcg gcaggaagaa gccggctccc aagaccgccg cggcaacgcc
    19801 cggagccccg tcgccaaggg ccgacagtgg ggggttgggc ggcatcgtgg ggggactcaa
    19861 gctccggtcc atcggaccgg cgctcaccga ggggcgcatc tccgacatcg ccgtggatcc
    19921 caccgacaag gcggtgtggt acgtggcgac cgcggcgggc ggcgtatgga agaccatcaa
    19981 cgacggcacc tcctacacgc cgatcttcga cggcgaaggg tcgtggtcga tcggcgcgat
    20041 cacgatcgat cccaacgatc acaacgtcat ctgggtcggg accggcgaga acaacgcgca
    20101 gcgggtggtg gccttcgggg acggcgtcta caagtccgtc gacggcggca agagctggaa
    20161 gaacgtcggg ctcaaggagt ctcagcacat cggccggatc gtcgtggacc cccggaactc
    20221 cgacgtggtg tacgtcgccg cccagggacc cctgtggagt ggcggcggcg accgcggcgt
    20281 cttcaagacc accgacggcg gcgcgacctg gaccaaggtc ctggccggcg acgactggac
    20341 gggcgccaac gacattcagc tcgatccccg ccatcccgat gtcctcgtcg cgaccacctg
    20401 gcagcgcgag cgccgcgtct acggattcat cgcgggtggc cccggttcgg cggtctggcg
    20461 gtcgactgac ggcggcaaga cctgggtgaa gtcccaatcg ggatttccca aaggcgacct
    20521 cggccggatc ggtttgtcgc gctcgcctgc cgaccccgac gtcatctacg ccatcgcgca
    20581 ggccgccgac gagaagggcg gattgttccg gtcgcacgac ggcggggcga gctgggagaa
    20641 gatgagcggc acgcagttcg gcgggaacta ctacaaccgg gtgttcgcgg acccgaagaa
    20701 cgtggaccgc gtgtatgcgg tgaatgtgtt cctgcagatc tcgaacgacg gcggcaagac
    20761 cttcagctcg ctcggcgaaa tcaacaagca cgtcgacaac cacgtggtct ggatcgaccc
    20821 cgacgacacc gaccatctgg tcgtcggctg cgacggcggc gtctacgaga ccttcgacca
    20881 cggcaagagc tggcggttcg gcgccaacct gccggtgacg cagtactacc gcgtggcgac
    20941 ggacaacgcc gagccgttct acaacgtgta cgggggcgcg caggacaact tctcggtcgg
    21001 cgggccgagc cggacgcgca cgaacaacgg catccggaac tccgactggt tcatcaccgc
    21061 gggcggcgac gggttcggga gcgtggtcga ccccaccgat cccaacaccg tctatgcgca
    21121 atcgcagttc ggcaacctcg tccggttcga tctcaggacg ggcgagcagg tggccatcca
    21181 gcccttcgac gagaccaccg gggaagccct ccgctggaac tgggacgcgc cggtgatcat
    21241 cagcccgcac gaccatcacc gtctgtactt cgccgcgcag cgggtgtacc ggagcgacga
    21301 ccgcggtgac tcgtggcgcc cggtgagccc ggacctgtcc cggcaaatcg accggaatgc
    21361 gctgaagatg atggggcggg tgtggggggt agatgcggtg gagaagaatg tgtccaccac
    21421 gttgttcggt acgatcttca ccctcgccga atcgccgctc aaggaggggc tgctcttcgc
    21481 cggtaccgac gacgggcgca tccagatcag cgaggatggc ggagcgcact ggcgggccac
    21541 ggaccatttc cctggcgtgc ccgacacgac cttcgtgacg cgcgtcgtgc cgtccacccg
    21601 cgacgtgaac accgtgtacg ccacattcaa cggtcactgg tcgggggact tcaagcccta
    21661 cgtcctcaag agcaccgacc tgggccgcac ctggaccaac atctccggtg acctgcccga
    21721 gcgtggcgac gtctggtgcc tcgccgagga cggggtggat cccaatctgt tgttcgtggg
    21781 caccgagttc gggctctact ttacggccga tggcggccag aagtggaccc ggctccagaa
    21841 cggattgccg acgattcagg tccgtgacct gaccatccag cggcgcgaga acgacctcgt
    21901 gctcgcgacc ttcggccgcg gcttctacgt gctggacgac tacacacccc tccggcacct
    21961 gagctccgac aacctggcgc agcgggcggt cattttcccg gttcgtccgg cgccgttgta
    22021 cgtgccgtcg agtccgctgg gcctccccgg cgtcggattt cagggggcgg ggcactactc
    22081 ggcggacaat ccgccgtacg gcgcggtgtt ctcctacttc ctgaaggacc ccatcctttc
    22141 ccggaaggag cgccggcaaa aggccgaggc cgcgaaggag aaacagggtg ccgatatcgg
    22201 gtatccgtcg tgggattcgc tgaaggcgga ggaccgcgag gaggatccgg cgctgatcgt
    22261 gaccgtctcg gactccgccg ggaatgtcgt gcgccggttc acgggatcga ccagcaaggg
    22321 catcaaccgg gtggcgtggg acctgcggtt gccgaccagc aatccagtgg acggaccgcc
    22381 gttcaaggct gatccgaaca atccgttcgg ctcgggccca aggggaccct acgtgccgcc
    22441 ggggacttat caggtgtcac tcgccgagcg ggatgacagc gtcttcacgc cgctggggac
    22501 gccgcagcgg ttccgcgtgg tggatatcga cagcgccctg ccgggccggg cgtacgcgtc
    22561 tctgagtgat caggagaggg tggcggccct tcagcgggag gtcctgggcg cgtcggcgct
    22621 cgtcaacgag gcgttgacca gggtcggata tctcaagcgg gcgatcgacg aaacgcccac
    22681 ggcggatgcg acgctcgcga gccgggcgcg ggtgatggaa aaccgtctgc gtgacgcgcg
    22741 cgagctcttg tcgggcgatc cgacgttggc gcggcatcag gaaccgtcgc cgcagtctct
    22801 cctggaccgt ctgcgcggcg cggtgggggg ccgctcgggg agctccctgg cgcccatgaa
    22861 tgccgcggag cgtgcgcagt tcgattacgt gcgcacgcag ttcagcgtcg tgatgccgaa
    22921 gattcagcgg ctgatcgacg tcgagctcaa gagcttggag gatgcggcgg aggcggcggg
    22981 ggtgccgtgg acgccgggaa ggatgccgaa ggcgccctaa gattgccccc ggtggattcg
    23041 aaccaccatt ctcggatcca aagtccgatg tcctgccgtt ggacgagggg gcagcggtgt
    23101 tgccggacct aacttagcga ctgcgcctcg gccggagcag ggggcaagga ccccgctggt
    23161 cagtcagggt tctggttgta tgtgaccgcc tctttgatca gcgcctggaa cgccttctcg
    23221 ttgatcttcg cgccttctgg gaaatcgatg gcccgccgag tcccgccctc gagactcgag
    23281 ttgaaaagct ttgacgggtc tttgagcgcg gctccgtgag ggaaggtcag tttcaccgcc
    23341 ctcttgtacg tctcgccggt acagatcatt ccatcgtgct tccacaccgg caccgcccat
    23401 ttccagtctt caaccacgtc cgggtccgcc tgcttgatca gcttccggag gcgggcgagc
    23461 atctcgccgc gccagtcggc cagctcttcg atgcgccggt cgatcatcca ggacgcggtc
    23521 tttccgggcg gcggggaact cttcttggcc atgcgtgcct aaagacagag atagggtcga
    23581 gcggggccgt tccgaactag gtggaaaggc cccaaggtgt cggcggcaag tgcgacggct
    23641 ttgatcttca cggagacctc tccgaaggct ctttgaatat tacacgcgaa cgggttttcc
    23701 caccgtcacg ggtgagccgg cgacgacaaa tgcggtcacc ggattggcca gcgccgtgcc
    23761 cagggtcggg ccgtacaaac ctgggaaatc ggcgtcgatc cggacatccg tggcctgccg
    23821 aacgggccag cggtcgtgct ggacccggta ctcgatcgtc ccgccgtccc gctgtcgggt
    23881 atagccccac tcgtggtcgg tgatgaactc ctcatggctt ccggctcggg ggatggcggc
    23941 gctatccgtg aacgtggccg tcaatccctc ccagcgtcca tcgcgtcgcc attcgtgtcg
    24001 aacagcccca tcccggacag agctccgcat cggcaagctc acgtagggtt cgttgtagat
    24061 gaggcgggcg atcagcgcga tcgctcgccg gggcaccacc tcgcgaatga acacgacccc
    24121 gcgccggacc tccccggcca ccgtccggcg cacatagaac ctcagattga gctcgggaaa
    24181 gtcgcgatgg aaggggatgg gaagccccag tactcgcgtg ttcaggaact ggaacgcgac
    24241 gacgctcgcg tagcaatgcc cgtcatggct gtcgacgtgg gttcccgctg ggaggtgcgg
    24301 ctcgaggagg gcagggtcca cctggtagtt cagcatcacg agatggcacc actcggcggt
    24361 cagaaagggg cggggcatat cacaaacttc cacttctttt ccttacatac aacgcggcct
    24421 gtcatccctt cgcttcgctc agggcgagct ctgagcgccg aaggcgcgaa g
//
LOCUS       DASUBP010000127         2082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_85421, whole genome shotgun sequence.
ACCESSION   DASUBP010000127 DASUBP010000000
VERSION     DASUBP010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85421"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1911
                     /locus_tag="VFU75_04835"
     CDS             <1..1911
                     /locus_tag="VFU75_04835"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEU5466191.1"
                     /translation="NTINLQEQLVGKDLPILARALATGDHTPTFALLKGWRNYLCLAR
                     LEQTRAVPDSLFEDERASELQRLAEWAAHSADGSLSDLADEPSDEVWDAVAAESDLCT
                     RLKCPFFDRCFVFRARRAAAEADVVVVNHHLLASDLAVRMASDNWVDAAVLPPYRRLI
                     IDEAHHLEDVSAAHLGAQVSQVGITRLLGRFERNGRGLLPTLSRELTHRDDSPLDAKG
                     RDLIQRRLLDSLRTARRSSEELFVLLDRRLTEPVLRLTDDFAKDGVWTEGLGVALENV
                     LLAFNQLRDGVESIADRLSLDDRSERGGQLIAEMRGVVRRLETAAAGLTAALKPAPGG
                     PPAVRWIERKGRRATNLGLSAVPLDLAPILKDALFDRVETVVLTSATLAAGGEFTFLE
                     ERLGLDLPPSRVVVREVLDSPFDFAAQCVLGIPTDLPEPRDDEAGHDAAVARVLLEVA
                     RASDGGVFCLFTSHGALRRTADAVRTEVGARWPLLVQGEAPRDHLLRRFRESGSAVLL
                     GTDSFWEGVDVPGRALRALILAKLPFKVPSEPLTAARLERLAEKGVDGFTHYLIPHAA
                     LKLKQGFGRLIRSRSDVGAVLLLDRRVVTKRYGPLLLEGLPGAARAVGNWHDVRDECE
                     EFFARHGIGEPV"
BASE COUNT          311 a          670 c          746 g          355 t
ORIGIN      
        1 aacaccatca acctgcagga gcagctggtc gggaaggacc tgcccattct ggcgcgcgcg
       61 ctcgcgacgg gcgaccacac gccgaccttc gccctgctca aggggtggcg gaactatctg
      121 tgtctcgccc gcctcgaaca gacccgcgcg gttcctgatt cgctcttcga ggacgagcgg
      181 gcgtcggagc tccagcggct cgcggagtgg gcggcgcatt ccgcggacgg atcgctgtcg
      241 gatctggcgg acgaaccgtc ggacgaagtg tgggatgccg tcgccgcgga gtccgatctc
      301 tgcacccggc tcaagtgtcc attcttcgac cggtgcttcg tcttccgcgc ccggcgtgcc
      361 gccgcggagg cggacgtcgt cgtggtgaac catcacctcc tggcttcgga cctcgcggtc
      421 cggatggcct cggacaactg ggtcgatgcc gcggttctgc ccccctaccg ccggttgatc
      481 atcgacgaag cgcatcacct ggaggacgtg tcggccgctc atctgggcgc gcaggtgagc
      541 caggtcggga tcacgcgact gctcggccga ttcgagcgca atgggcgggg gctgttgccg
      601 acgttgtccc gtgagctgac ccatcgcgac gactccccat tggacgcgaa gggacgtgac
      661 ctcatacagc ggcgcctgct cgactccctg cgcaccgccc gccggagcag tgaggaactg
      721 ttcgttcttc tcgaccggcg tctgaccgag ccggtgctcc ggctcaccga cgatttcgcg
      781 aaggacggcg tgtggaccga aggactcggg gtcgcgctgg agaacgtcct gcttgcgttc
      841 aatcagttgc gggacggggt cgagtccatc gccgaccggc tctccctgga cgaccgaagc
      901 gagcggggcg ggcagttgat cgccgagatg cgcggcgtgg tgcggcggct cgagacggcg
      961 gccgcggggc tcaccgcggc gctgaagccg gcgcccggtg gtccacccgc ggtccgctgg
     1021 atcgagcgaa agggcaggag ggcgacgaac ctcggattgt ccgccgtccc gctcgacctc
     1081 gcgccgatcc tgaaggatgc gttgttcgac cgggtggaga cggtggtgct cacctccgcg
     1141 accctcgccg cgggtggcga gttcacgttc ctcgaagagc ggctgggcct cgatctgccg
     1201 ccctcccgag tcgtggtgcg cgaggtcctc gactctccat tcgattttgc ggcccagtgc
     1261 gtcctcggca ttcccaccga ccttcccgag ccacgcgacg atgaggcggg gcacgacgcc
     1321 gcggtggcgc gggtactgct cgaagtcgcg cgggcgtccg atggcggagt gttctgcctt
     1381 ttcacgagtc atggggccct ccggagaacg gcggacgcgg tccgtaccga ggtcggagcg
     1441 cggtggcctc tgctggtgca gggggaggcg ccccgggatc atctcctccg gcgcttccgg
     1501 gaatcggggt cggcggttct gctcggcacc gactcgttct gggagggcgt ggacgttccg
     1561 gggcgggcgt tgcgggccct cattctggcc aagctgccct tcaaggtgcc gagtgaaccg
     1621 ttgacggccg cgcggctgga gcgcttggcc gaaaagggcg tggatgggtt cacgcactac
     1681 ctcatcccgc acgcggcgct caagctcaaa cagggattcg gaaggttgat acgcagcagg
     1741 tcggacgtcg gagccgtact tctcctggac cggcgcgtcg tcacgaaacg ctacggcccg
     1801 ctcctcctgg aaggactgcc gggggcggcg cgggcggtgg ggaattggca cgatgtccgg
     1861 gacgagtgtg aagaattctt cgcccgccat gggatcggcg aaccggtgtg aaacgtctgg
     1921 cgctgagggg tagattcaag gcatgagaga gttgagcaag cggatcgaga ccctccccgg
     1981 gtacccgatg gcccagatcc cggccatcaa gaaacggctt ctggaaaagg gagtcgacgt
     2041 catcgatctc ggggcggggg acgccgattt cccggctccg ca
//
LOCUS       DASUBP010000128         3525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_425759, whole genome shotgun sequence.
ACCESSION   DASUBP010000128 DASUBP010000000
VERSION     DASUBP010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3525
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425759"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1512
                     /locus_tag="VFU75_04840"
     CDS             <1..1512
                     /locus_tag="VFU75_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466192.1"
                     /translation="DTPTDLRYGAIVEVEQYWQPLGYATGDLLYSAALAPGDEARIAI
                     LDGRWGDAGPPRERPLQTLARLIGSPLLSDLVSEGSAAWPLDPLTIRPEGDPANALAQ
                     GATDTVRHLNERAARVAENVRRAPFRVGDVRADTPPDASIRTVKNPRTDRLMTYHFFE
                     PLQRYRVVARTARVRPAVLVPFRLPSQAVRAMVRQYGHVLRRVLLDRTLLAELDALLG
                     LGTTGAGADWERAGTTPPISELRLIVEPGTETAGQLAQIFCYLHADATRYTVHFFPAE
                     AARGPLAQDHPEANQWIGAIRLADFHQHPLRYPGELAIENGSPASALFQTLHVEGRKG
                     DAWRRLMTVQNLLVPGQSRMPLASLAAMVESVGLASQESRLLAHITANLPHYAAAIIA
                     AGDPAVRHLALSRVRDAEGRAVADVIENQVAGAQGSYLAFPLLSADLAPPAVAAALHQ
                     YGARGSRVPDEVVVTLPLPGVWLSAQASGTIEGLAEEEDARERGLRGRRLNQA"
     gene            1668..1907
                     /locus_tag="VFU75_04845"
     CDS             1668..1907
                     /locus_tag="VFU75_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466193.1"
                     /translation="MPVPIGRFVQELEKLKKEVDAGSLRHGEYDQRLARVLGELRDEK
                     LDADRPKVAAALTDLLARGVITPGVKTHIEKRLGL"
     gene            complement(1909..3339)
                     /locus_tag="VFU75_04850"
     CDS             complement(1909..3339)
                     /locus_tag="VFU75_04850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU5466194.1"
                     /translation="MSEAVIESTGEHQAHQSGGWWATIREALRGSHQDYTTGPVGRAI
                     LLLAIPMVLEMLMESIFAVADVFWVSRLGPDAVATVGITESLLTLIYTAAMGLSIGVM
                     AMVARRIGEKNPAGASEAAVQGIGLGIGASVLIAAIGVPLAPWLLHLMGAQPEVLVHV
                     SYARVMLGGNVTILLLFLINATFRGAGDAAIAMRTLWLANGINILLGPCLIFGVGPFP
                     RLGVVGAAIATNIGRGTGVMYQLYRLLRKDARVVMHRADLVLRPAVMKTLLKLSGSGT
                     LQVFIGTASWIGLIWILAGFGTDVLAGYLIGIRLIVFALLPSWGLSNAAATMVGQGLG
                     AGNPERAEQAVWRAGLYNMVFLGLVGLAFIAGAEPLIGLFRQVPQVSEVGVQCLRIVS
                     YGFLFYAFGMVLTQSFNGAGDTWTPTFLNLFCFWFWEVPLAYVLSRTLGWGPKGVFAA
                     ITIAFSTIAVASAILFRRGRWKTRRV"
BASE COUNT          597 a         1237 c         1172 g          519 t
ORIGIN      
        1 gacactccca ccgatctccg ctacggagcc atcgtcgagg tcgagcagta ctggcaaccc
       61 ctcggctacg cgaccggcga cctcctgtac agtgccgcgt tggcccccgg tgacgaagcg
      121 cgcatcgcga ttctcgacgg gcgttggggc gacgcgggcc caccgcgcga gcggcccctc
      181 caaacgctcg cgcggctcat cggctccccc ctgctgtcgg acctggtgtc ggagggctcg
      241 gccgcctggc cactcgaccc gctgaccatt cggcccgaag gggatccggc gaatgcgctg
      301 gcgcagggcg ccacggacac ggtgcggcac ctgaacgagc gtgcagcacg cgtcgcggag
      361 aacgtgcgcc gggccccctt ccgggtgggt gacgtccggg ccgacacccc tcccgacgcc
      421 tcgatcagga ccgtgaagaa tccgcggact gatcgcctga tgacgtatca cttctttgag
      481 ccgctccagc gctaccgggt ggtggcgcgg acggcgcggg tgcgcccggc ggttctcgtt
      541 cccttccgcc tgccgagcca ggcggttcgc gccatggtcc ggcaatacgg ccatgtattg
      601 cggcgggtcc tcctcgaccg cacgctcctg gccgagctcg acgccttgct gggcctgggc
      661 acgacgggag cgggagccga ctgggagcgg gcggggacga cgcctccgat ctccgagctg
      721 cgcctgatcg tcgagccggg aacggagacc gccggccagc tcgcgcagat cttctgttac
      781 ctgcacgccg acgcgacccg gtacaccgtg catttcttcc ccgccgaagc cgcgcgcggg
      841 ccgttggcgc aggatcatcc tgaagcgaat cagtggattg gcgcgatccg gctcgcggac
      901 ttccatcagc atccccttcg ctatcccggc gagctggcga tcgaaaatgg ctcaccggcg
      961 tccgcgctgt ttcagacgct gcacgtcgag gggcggaagg gagatgcatg gcgccgtctg
     1021 atgacggtcc agaatctcct ggtgccgggt cagtcccgca tgcccctcgc gtccctggcc
     1081 gccatggtcg aatcggtcgg tctcgcgtcc caggagagcc gccttctggc gcacattacc
     1141 gccaacttgc cgcactatgc cgcggcgatc atcgcagcgg gcgatccggc ggtgcgccac
     1201 ctcgcgctct cgcgggtacg ggatgcggaa ggccgggcgg tggccgacgt catcgagaac
     1261 caggtggctg gggcgcaggg gagctacctg gcgttccccc tgctctccgc cgaccttgcg
     1321 ccgccggcag tcgccgccgc cctgcaccaa tacggcgccc gcggatcccg ggtccccgac
     1381 gaggtggtcg tgaccctgcc gctgcccggc gtctggctct cggcgcaggc gagtgggact
     1441 attgaggggc tggcggaaga ggaggacgcc cgggagcgag ggctccgcgg gcgccgcctc
     1501 aaccaggcgt aggaccagca cggtaatcag ggatgatgcc ggtgcctcgg tgaccccggg
     1561 ctggcgcccg gggttatgaa tacctgttcc gatttcgatg tacataaccc ccagcgccag
     1621 ctgggggaca cgacgccgac ctcacctcat cgaggagtgc gcacccaatg cccgtgccga
     1681 tcggtcgatt cgttcaggag ctggagaagc tgaagaagga agtggatgcc ggctccctgc
     1741 ggcacggcga gtacgaccag cgtctcgccc gcgtgttggg ggaattgcgg gacgagaagc
     1801 tggacgccga ccgcccgaag gtggccgccg cgctcaccga cctcctggcg cgcggcgtca
     1861 tcaccccggg cgtgaaaacc cacatcgaga agcggctggg tctgtaggtc agacccgccg
     1921 cgtcttccag cggccccgcc ggaacaggat cgccgacgcc accgcgatcg tggagaacgc
     1981 gatcgtgatg gcggcgaaca cacccttcgg cccccacccc agcgtccgag acagcacgta
     2041 cgcgagcggg acttcccaga accagaagca gaacaggttc aggaaggtcg gcgtccaggt
     2101 gtcgccggcg ccgttgaatg actgcgtgag caccatgccg aacgcgtaga acaggaatcc
     2161 gtagctcacg atccgcaggc actgtacacc cacctcggac acctgaggta cctggcggaa
     2221 gagcccgatc agcggctccg cgccggcgat gaaggcgagt cccacgaggc ccaggaagac
     2281 catgttgtag agccccgcac gccacacggc ctgttccgcg cgttcgggat ttccagcccc
     2341 cagcccctgc cccaccatcg tagccgccgc gttcgacagt ccccaggagg gcagaagcgc
     2401 gaacacgatg agtctgatcc cgatcagata ccccgccagg acgtcggtac cgaaccccgc
     2461 caggatccag atcaatccga tccagctcgc cgtcccgatg aagacctgga gcgtgcccga
     2521 gcccgagagc ttgagcaggg tcttcatcac cgccggacgc agcaccagat cggcgcggtg
     2581 catgacgacg cgcgcgtcct ttcgaagcag gcgatagagc tggtacatca cgccggtgcc
     2641 tcgcccgatg ttcgtggcga tcgcggcgcc cacgacaccc agccggggaa acggaccgac
     2701 cccgaagatc aggcacggac ccagcaggat attgatgccg ttcgcgagcc agagcgtgcg
     2761 catcgcgatg gccgcgtccc ccgctccgcg gaacgtcgcg ttgatgagga agagcaggag
     2821 aatcgtcacg ttcccgccca gcatgacgcg ggcgtaggaa acgtgcacga ggacctccgg
     2881 ctgcgcgccc atgaggtgca ggagccaggg cgcgagcggt accccgatgg ccgcgatcag
     2941 cacggacgca ccgatcccca gcccgatgcc ctgcaccgcc gcttcggagg cgcccgcggg
     3001 gttcttttcc ccgattcgcc gcgcgaccat cgccatcacg ccgatggaga gccccatcgc
     3061 cgccgtgtag atcagcgtga ggagcgattc ggtgatgccc acggtggcca cggcgtcggg
     3121 gcccagccgg gacacccaga atacgtcggc cacggcgaag atcgattcca tcagcatctc
     3181 gagcaccatc gggatggcga gcaggagaat ggcgcggccg acgggacccg tcgtgtagtc
     3241 ctgatgggac ccgcggaggg cttcgcggat ggtcgcccac cagccgccgg actggtgagc
     3301 ctgatgttct ccagtcgatt cgatcactgc ttctgacacg gaattgctcc ctgcattcga
     3361 tggttttctg acccccgacc cctgaccccc aaaaaccaat cgccccaccc ggccggcggc
     3421 acaggtgggg cgatcgggac gagcgtctgg ctcgtgctgc gatgctacct gtgtcgcgct
     3481 cctgtatcga cgacgcccag atccgggcgc acgacggcga ccggg
//
LOCUS       DASUBP010000129         4747 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1011712, whole genome shotgun sequence.
ACCESSION   DASUBP010000129 DASUBP010000000
VERSION     DASUBP010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4747)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4747)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4747
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1011712"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..664)
                     /locus_tag="VFU75_04855"
     CDS             complement(35..664)
                     /locus_tag="VFU75_04855"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466195.1"
                     /translation="MIVSEFMLQQTQVSRVLEYYPRFLKRFPTVYKLAAAKPRVVMEA
                     WEGLGYYARARNLHALAKEVSGVGGQVPSDPEELMKLPGVGRYTAGAVASFAYEKPVP
                     AVDTNVARVIARYFLGVGSREPSTPRQRNVRLPAAGSRELWDIAASIVPRNGRRAWAF
                     NQAIMELGARVCVARKPRCPECPVRSRCKTAGRQTGAQSLRRLAGRRNE"
     gene            816..1616
                     /locus_tag="VFU75_04860"
     CDS             816..1616
                     /locus_tag="VFU75_04860"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5466196.1"
                     /translation="MDTAAKLDLRRWAGRWSLRTAGHAGRFTYFLVDMARALTEWRIW
                     VPRTFEQAVLVGYGSLFIVLLIAGFAGAVTSLQTGYQFSGQFPKYIAGGVITESVILE
                     LGPVLTGLILAGRIGARYAAELGTMRVTEQIDALESLGRSPMSHLLLPRCIAGILVIP
                     ALVMFANATGFGVGWFAAKSSLGISNADFIYGARFFFRSFDLWYSVIKSFFFACAVVL
                     IPCYIGYNTKQGAEGVGRATTDAVVSASISILLLDVLLTQILLTGTGQ"
     gene            1613..2389
                     /locus_tag="VFU75_04865"
     CDS             1613..2389
                     /locus_tag="VFU75_04865"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5466197.1"
                     /translation="MIELKDVSKRFVDHVVLDHVDFHVDDGQTVALLGPSGVGKSVLL
                     KHIIGLITPDSGVVSVDGLNVASLKRKDLAHLRTRIGYVFQNGALFDSMDVRENIRLG
                     ITNGELYDDVEYCQERVKECLRLVNLQQEVAEKFPGELSGGMRKRVGIARAIAGKPKY
                     LLYDEPTSGLDPVNSDVIDGLIERLQDELGVTSVVVTHDVRGAFRVADKIALLWQAKI
                     RAVGTPEEIVASKDPAVQQFLERDLEMALIQSESTDGSDV"
     gene            2373..3254
                     /locus_tag="VFU75_04870"
     CDS             2373..3254
                     /locus_tag="VFU75_04870"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEU5466198.1"
                     /translation="MDLTYKQEVGVGGLVLAGLVVFVAGMFWLTGRSLSGNGITARVA
                     FTNVAGLKVGDPVMVSGVHRGRVAAVTLDRVGHVTVTLQLIAAEDRPKMDATATVAAG
                     DFFGGRFIDYFPGSDTSQFLPSNGMIQGTREEQLTDVAAGVATKANALMTSATNLVND
                     RLATDIHNTLVSTQHAMDVVSKAGSGPLLTQATATLASAERMMSHLDSLFTGPAGKRV
                     DTLTSNLAALSTQLNKATTSLSTLMSQIQQGKGTLGKMATDSSLYYHLDSTLVSLNAL
                     LKDLRERPGRYLTVKVF"
     gene            complement(3346..>4747)
                     /locus_tag="VFU75_04875"
     CDS             complement(3346..>4747)
                     /locus_tag="VFU75_04875"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HEU5466199.1"
                     /translation="DQPAAADTAAAHVFALETSLAKVSMSRISRRDPNANYHKMPIAE
                     ADALTPHLEWRAFMKESGLPPVDSVNIGQPSFFKGMDSIVAATPTADIKSYLRWHAIR
                     GAAPELTTAFRNEVFRYSTALSGVSVQQPRWKQCLQAANGGLPDAVTQAFIAKAFSPE
                     ARKRARDMVENLIAALDDRLHTLEWMSDSTRQAALVKLRAYGRKIGYADKFRDYATVE
                     IDKGPFALNAQRVAQYARSWRIAQIGKPVDHTFFSMTGPTVNANYNPTDNAITFPAGI
                     LQPPFFDPNADDAVNYGGMGAVIGHEMTHGFDDQGRQFDAHGNLRDWWTPADAAAYKT
                     RADKVANQFDTYTVVDSTTHVQGRLVLGESIADLGGLKIAFAAWQHSWAGKAHPAPID
                     GYTAEQRFFLAYAQIWREVATDQYLRNQVATDVHAPAKWRIIGPLSNLPEFAAAFHCQ
                     AGDPMVRPDSLRAAIW"
BASE COUNT          787 a         1536 c         1571 g          853 t
ORIGIN      
        1 tgcagtagtg cagaattcag gaagggcttt cccgtcactc gttcctacga cccgctaggc
       61 gcctgagcga ctgggcgcct gtctgtcgac cagccgtctt acacctactc ctgaccggac
      121 actcagggca tctcggtttc ctcgcgacgc agactctcgc tcccaattcc atgatcgcct
      181 gattgaatgc ccacgctcgc cgaccgttcc tcggcacgat ggacgccgcg atgtcccata
      241 actctcggga gccggcagcc gggagccgta cgttccgttg acggggagtg gacggctccc
      301 ggctcccgac tcccaagaag taccgagcaa tcaccctcgc cacattggta tccaccgccg
      361 gcaccggttt ctcgtaagcg aagctggcca ccgcccccgc ggtgtaccgc ccaacccccg
      421 gcagcttcat gagctcttcg ggatcacttg gcacctgacc cccgacccct gacacctctt
      481 tcgcgagcgc atggagattc ctggccctgg cgtaataccc aaggccttcc cacgcctcca
      541 tcaccacccg cggtttcgcc gccgccagct tgtagaccgt cggaaagcgc ttgagaaagc
      601 gcgggtagta ctcgagcacc ctcgagacct gagtctgctg gagcatgaat tccgagacga
      661 tcacctcgta ggggtcgcgc gtccgcctcc acgggagatc ccgggcgctc ttccggtacc
      721 aggccaggag cgcgcgcgta aaccgttgcg cggacggtgg tttgcgcgtg gtgggggtag
      781 acttagcggg catgggtacc caagatacac gcaccatgga caccgcagcg aagctggacc
      841 ttcggcgctg ggccgggcgg tggagcctca ggaccgccgg tcacgccggg cgtttcacct
      901 acttcctcgt ggacatggcc cgcgccctca ccgagtggcg gatctgggtg ccccggacgt
      961 tcgagcaggc cgtgctcgtg gggtacggtt cactgttcat cgtcctcctg atcgccgggt
     1021 tcgccggtgc cgtgacgtcc ctgcagaccg gctaccagtt ctccggacaa ttcccgaagt
     1081 acatcgccgg cggcgtgatc actgaatcag tgatcctgga gctcggcccc gtgctcaccg
     1141 gactcattct cgcgggccgc atcggcgcgc ggtacgccgc cgagctgggc accatgcgcg
     1201 tgaccgaaca aatcgacgcg ctcgagagcc tcggccgctc gcccatgagt cacctgctgc
     1261 tcccccggtg catcgcgggc attctcgtga tcccggcact ggtgatgttc gcgaatgcga
     1321 ccggattcgg cgtcggctgg ttcgcggcga agtcgtcact cggcatctcc aacgcggact
     1381 tcatctacgg cgcgcgattc ttcttccgct cgttcgatct ctggtactcg gtgatcaagt
     1441 cgttcttctt cgcctgcgcg gtggtgctga tcccctgcta catcggttac aacacgaagc
     1501 agggtgccga gggcgtcggc cgcgccacga cggacgcggt ggtttcggcg tcgatctcca
     1561 tcctcctgct cgacgtgctc ctcacgcaga tcctcctgac gggcacgggt cagtgatcga
     1621 gctcaaggat gtctccaagc gcttcgtcga tcacgtggtg ctcgaccacg tggacttcca
     1681 cgtggacgat ggccagaccg tcgcgctcct ggggccgtcc ggtgtgggga agagcgtcct
     1741 gctcaagcac atcatcggac tcatcacgcc cgattccggg gtcgtttcgg tcgatggcct
     1801 gaacgtcgcg tcgctcaagc gcaaagacct cgcgcacctg cgcacccgca tcgggtacgt
     1861 cttccagaat ggcgcgctct tcgactccat ggacgtgcgg gagaacatcc ggctcggcat
     1921 cacgaacggc gaactgtacg acgacgtcga gtactgccag gagcgcgtga aggagtgcct
     1981 gcgcctggtg aacctccagc aggaggtcgc cgagaagttc ccgggggagc tgtcgggcgg
     2041 catgcgaaag cgcgtgggca tcgcgcgggc gatcgcggga aaacccaagt atctcctgta
     2101 cgatgagccg acctcggggc tcgaccccgt gaactcggac gtgatcgacg ggctgatcga
     2161 gcgcttgcag gatgagctgg gtgtcacaag cgttgtggtg acgcacgacg tccgcggggc
     2221 cttccgcgtg gccgacaaga tcgccctgct ctggcaggcg aagatccgcg cggtgggcac
     2281 accggaggag atcgtggcgt cgaaagaccc ggcggtgcag cagttcctgg aacgcgatct
     2341 ggaaatggcg ctcattcaaa gcgagagcac cgatggatct gacgtataaa caggaagtag
     2401 gcgtcggcgg cctggtcctg gccggactcg tggtcttcgt ggccgggatg ttctggctca
     2461 ccggccgctc cctctccggc aacgggatca ccgcgcgcgt ggcgttcacg aacgtggccg
     2521 gtctcaaggt cggcgacccc gtgatggtct ccggcgtcca ccggggccgg gtggcggcgg
     2581 tgacgctgga ccgggtgggg cacgtgacgg tgaccctgca gctcatcgcg gcggaggacc
     2641 gcccgaagat ggacgcgacg gccaccgtcg ccgcgggcga cttcttcggc ggccgcttca
     2701 tcgactactt ccccggttcg gacacatccc agtttctgcc ctcaaacggg atgattcagg
     2761 ggacccgtga ggagcagctc acggacgtcg cggcgggcgt ggcgaccaag gccaacgccc
     2821 tgatgaccag cgcgacgaat ctcgtgaacg accgcctggc gaccgacatc cacaacacgc
     2881 tggtctcgac tcagcacgcc atggacgtgg tgagcaaggc cggctcgggc ccgttgctca
     2941 cgcaggccac ggcgacgctc gcgtccgccg agcggatgat gtcacatctg gattctctgt
     3001 ttacgggacc ggcgggcaag cgggtggata cgctgacgtc gaaccttgcc gcgctttcca
     3061 cccagctcaa caaggcgacg acgtcgttgt ccaccctgat gtcacagatc caacagggca
     3121 aggggacgct cggcaagatg gcgacggact cgagcctcta ctaccacctc gactccacgc
     3181 tcgtctcgct caatgcgttg ctgaaggatt tgcgcgagag gccgggaaga tatttgacgg
     3241 tgaaggtgtt ctagggaaga gggagggggg aggagtcgca gtcagagcag tggagacccc
     3301 acttcctgga ctgcgacccc tccctcctcc cccctcccag gtttcctacc aaatcgccgc
     3361 cctcaacgaa tccggcctca ccatcggatc tcccgcctga caatggaacg ccgccgcaaa
     3421 ctccggcagg ttggagagcg ggccgatgat gcgccacttc gcgggcgcgt ggacgtccgt
     3481 ggccacctgg ttgcgcaggt actgatcggt cgcgacctcg cgccagatct gcgcgtacgc
     3541 caggaagaac cgctgctcgg cggtgtagcc gtcgatgggc gccggatgcg ccttgccggc
     3601 ccatgagtgc tgccaggcag cgaacgcgat cttgaggcca cccaggtccg cgatcgactc
     3661 gccgaggacc aggcgaccct ggacgtgggt ggtggagtcg acgacggtgt aggtgtcgaa
     3721 ctggttcgcg accttgtcgg ctctggtctt gtaggcggcc gcgtcggcgg gcgtccacca
     3781 gtcgcgcagg ttcccgtggg cgtcgaattg ccggccctgg tcgtcgaagc cgtgggtcat
     3841 ctcgtgaccg atcacggctc ccatcccgcc gtagttcacc gcgtcatccg cgttgggatc
     3901 gaagaagggc ggctggagaa tgccggccgg gaacgtgatc gcgttgtcgg tcgggttgta
     3961 attcgcgttc accgtggggc cggtcatgga gaagaacgtg tgatccaccg gcttgccgat
     4021 ctgcgcgatt cgccagctcc tcgcgtactg cgccacgcgc tgggcgttga gcgcgaaggg
     4081 gcccttgtcg atctcgacgg tggcgtaatc ccggaacttg tccgcgtagc cgatcttgcg
     4141 gccgtaggcg cgcagcttca ccagcgcggc ctgccgggtc gagtcactca tccattcgag
     4201 ggtgtggagc cgatcgtcga gcgcggcgat gaggttctcg accatgtccc gagcgcgttt
     4261 ccgggcttca ggcgaaaagg ccttggcgat gaaggcctgc gtaacggcgt cggggagtcc
     4321 gccattcgcg gcctggaggc actgcttcca ccggggctgc tggaccgaaa cgccggagag
     4381 cgcggtggag taacggaaga cctcattacg gaaagccgtg gtgagctcag gtgcggcgcc
     4441 acggatcgcg tgccaccgga gataggactt gatgtcggcg gtcggcgtcg cggcgacgat
     4501 ggagtccatg cccttgaaga atgaaggctg gccgatgttg accgagtcga cgggcggaag
     4561 ccctgactct ttcatgaacg cccgccactc gaggtgaggc gtgagcgcgt ccgcctcggc
     4621 gatcggcatc ttgtggtagt tcgcgttcgg atcgcggcgc gagatgcggg acatcgagac
     4681 tttcgcgagc gaggtctcga gcgcgaagac gtgcgccgcg gcggtgtcgg cggccgcggg
     4741 ctggtcg
//
LOCUS       DASUBP010000130        12882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1147912, whole genome shotgun sequence.
ACCESSION   DASUBP010000130 DASUBP010000000
VERSION     DASUBP010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 12882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12882
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1147912"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1421)
                     /locus_tag="VFU75_04880"
     CDS             complement(<1..1421)
                     /locus_tag="VFU75_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466200.1"
                     /translation="MNVSPLAGHPAPPELLLDISKLVTAYYTRIPDPAVPAQRVVFGT
                     SGHRGSAFETTFNEWHILAISEALCRYRTSQGIDGPLFLGIDTHALSVPACATALEVL
                     AAHGVDVMLAEDDEYTPTPAVSHAIIGYNRGRAEGLADGVVITPSHNPPDNGGFKYNP
                     PHGGPADTDVTGRIEAGANEIMAQGLKGVRRMPYERALRAPTIHRYDYRTAYVNDLGQ
                     IVDLDLVREAGIRVGVDPLGGAGVHYWGAIAEHYRLNLKVVNDAVDQTFRFMSVDWDG
                     RIRMDPSSSYAMQRLIGLKDHYDIAVACDTDHDRHGVVARSTGLLPSNHYLAVAIDHL
                     LRHRPDWGQGTGVGKTVVSSAMIDRVCDRVGRRLYEVPVGFKWFVAGLSDGSLGFCGE
                     ESAGATFLRRDGTVWTTDKDGIVAALLAAEITARAGRDPGEIYGDLARELGESSYERV
                     EAPATADQRRILAALTPNQMQCS"
     gene            complement(1418..2167)
                     /gene="pgl"
                     /locus_tag="VFU75_04885"
     CDS             complement(1418..2167)
                     /gene="pgl"
                     /locus_tag="VFU75_04885"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HEU5466201.1"
                     /translation="MSDGGGIRPGVTVLADVEALADAAAERVTQHLTAAIQGRGQAVV
                     VLAGGSTPRPTYERLGGEGFASRIPWPCVHVVWGDERCVPPDDVESNYRMARTAWLDR
                     VPIPPANLYRIHGEDDPAVAARAYEATLRDLVRAVGPPDLVVLGLGEDGHTASLFPGS
                     AAARERTRWVMPEYQAATATWRVTLTPVVINTTAEVLFLVSGAHKAEILKRVLQGPRR
                     PDELPAQAIAPSGGRVDWLVDRAAAAGLEHL"
     gene            complement(2164..10959)
                     /locus_tag="VFU75_04890"
     CDS             complement(2164..10959)
                     /locus_tag="VFU75_04890"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HEU5466202.1"
                     /translation="MRVPLPIFFPSLPPLEDPIRAELFGIERLEQHAESLAAAQPVAA
                     GREPQKPLLPRVDDNGRVLREANRTIAEAVREEHWITPAAEWLVDNFFVVDEQLREIR
                     DDLPVGFYRELPALAEGPLTGYPRVYGIAWAFVAHTDSRFDPETLRRFVKAYQRVQPL
                     NIGELWALTISLRVVLVENLRRVADAMVRGRAARREADALADELLDRRGQGLQSVAAV
                     LSRDDSPRLVTAFAVELVQRLRDQDPAVTPALTWLRQRLAAQGTSAEEIALVEHQRQA
                     AMTVTVRNIITSMRLMSALDWTAFFESVSLVDEALRAGSAFAAMDFTGRDSYRHAIEA
                     LARGSGRTELEVTQAALAAAARSRSGSAGRGGALDERGGDPGYYLVGSGRREFERVVH
                     FRPPWPLRLRRAFIDAATPGYLGTLLLVTVAILAVPLVLTAWAAAPSWVLLVLGVLGL
                     IPASDLAVALVNRFVNTVVAPRPLPRLEWAAGVPEHCRTLVAVPTLLTDAADAEEQVE
                     RLEVHYLANSDGDVRFALVSDWLDAGTEHAAGDEETLSAARTGVARLNARHGPAPGGG
                     DRFFVIHRRRLWNAKQHAWMGWERKRGKLHELNRLLRGARDTTFIAADGEMPAAPAGV
                     RYVITLDADTRVPIGAVQRLVGTMAHPLNRPRLDGATRRVVEGYAILQPRVTPPLPGR
                     GGSRFQRLSSGPAGIDPYAAAVSDVYQDLFGEGSYTGKGIYDVDAFEAALEGRVPDNT
                     LLSHDLFEGLFARAGLVTDVELLESPPANYLAAAARQHRWARGDWQLLPWIVRERLSP
                     VGRWKMIDNLRRTLSLPAAFLTLVAGWLLPGPSPVLWTAFILAIIAAPPLLPVFAGLW
                     PRTPGIAKRSHVRAVVRDFRSAVSQMAVTLTTLPHQAWLMGDAILRTLGRVCLTHARL
                     LEWRTMTLTAAHAPTTVAGFYRRMSGGIVLTAIAAAAAAWRDPGALPVALPLLGVWGV
                     APAIMSWLSRPGPGPRTVPLSADDVRALRLIARRTWGYFTTFVTTESRDLPPDNFQET
                     PQPVIAERTSPTNIGLYLLSVVSARDLGWLGTLDAVDRIEATLRTIKGLEQYRGHLYN
                     WYDTRDGHPLEPRYVSTVDSGNLAGALIALGNACREMLGQGVPLPDARSGIADAAELL
                     RHALRSSGDEAEGQAHWERLTEALEVVMTVVGQPSGTPAAWAARLAELAAATQAVANR
                     ARDTGKGVSGQSVPLLSATEALQGAVETHERDLDTLMPWASSRPDDRAAPLMERLQGP
                     LTLAELPRICQAALEDLTVVRTAVAAGADGTPVRLRALDALAASLERSAAAATALIER
                     LNRLAAQASDMVGAMDFGFVFDRTRMLFAIGFRMEDRTLDPGRYDLLASEARLLSFIA
                     IAKGDVPVRHWFRLSRLLTPVGRDSVLLSWSGSMFEYLMPGLIMRSPLGSLLEQTCRL
                     AVQRQVEYGAERNVPWGMSESGYYARDLAMTFQYSSFGVPGLGLKRGLVENVVVAPYA
                     TALAAMVDPEAALRNLRALARAGADGRYGFYEAVDYTPSRLPAETEQAVVRMYMAHHQ
                     GMSVVALANVLTGGAMRARFHQEPMVRATELLLQERTPRDVPVVRPQTEADRVVGDVR
                     ELVPPQPRHFTSPDGATLRTQVLSNGRYAVMVTAAGSGYSRWRDLAVTRWREDVTCDD
                     AGSYILLRDVESDDRWSAGFQPTGSAPESYDVSFAEDRAEFSRRDGAITTRLEVIVSS
                     EDDAEVRRVSLTNNGARIREIDVTSYAEIVLAPAASDAAHPAFSNLFLETESVADRDT
                     LLATRRRRAPEDAEVWLAHVLAVEGETVGDLEWETDRAQFLGRGGTLRAPRAETEGGH
                     LSNTVGSVLDPIVSLRRRVRVRPGRTVRVAFTTLVGPSRAAVLDLADKYHDVTTFERV
                     ATLAWTQAQVQLHHLGIGPDEAHLFQTLGSALLYTDRALRGSTEVQARRGEGVTALWT
                     HGISGDLPIVLVEIDESDDIGIVRQLLRAHAYWRLKGLAVDLVILNDRAPSYEQDLQG
                     LIETLVRTNQSMPRPEGPDPQGRVIILRADRVTAGQRDVLESVARIDLSSRRGTLVEQ
                     VARAHRADPAPTVVTRREAAEVPVVAAPAMPSPRLEYFNGLGGFDAEAREYVITLRAG
                     QWTPAPWINVLANPDFGCQVSESGSGCTWSINSQENLLTPWSNDPVRNPPSDMFYIRD
                     DETGALWSPTPLPIREPGGEYVVRHGPGYTRFRYDGHDVTSDLLQFVPADDPVKISRL
                     TLTNHSPQPRHLSVTAYHDWVLGTSRSASAPFIMTELDPATGAMFARNSWSRDFASRI
                     AFADLGGGQVAATGDRTEFLGRNGALDRPAALIRRQPLSGRTGAALDPGCALQVTLEI
                     PAGESVSVVWLLGQTGTREQAQALIQRYRSDDLDRRLATVSGGWRAILETIQVTTPDR
                     ALDLMLNQWLLYQTLACRLWARTAFYQASGAYGFRDQLQDVMALAVARPDLMRAHILK
                     AASRQFVEGDVQHWWHEPAGRGVRTRISDDLLWLPYVVGYYLEVTGDQAILDEVVPFL
                     GGALLAAGQSESYFEPRPAAEHGTLFEHCARALERSLGVGAHGLPLMGTGDWNDGMNR
                     VGSQGRGESVWLAWFLHSVLRTWAPIAATRREAARAAAWGDHGVALRQAVEREAWDGN
                     WYRRAYFDDGTPLGGAGADACAIDSIAQSWSVLSGGAGAERARRAMASVDERLVRRSD
                     DLIVLLTPPFDRTALEPGYIKGYVPGVRENGGQYTHAAAWAVIAFAQLGDGAKAAELL
                     AMLNPISHTATAAGVLRYRVEPYVAVGDVYSEPPHVGRGGWTWYSGSAGWLYRAGIEW
                     LLGLRVRSGHVLVDPCIPSGWSGFTAILRHGEARYEIAVENPDGVSRGVALAELDGVA
                     LDHRVGVPLVGGKGVHRVRVVMGASGAPRIPGGPADGGPVEALAE"
     gene            complement(10994..12046)
                     /gene="gnd"
                     /locus_tag="VFU75_04895"
     CDS             complement(10994..12046)
                     /gene="gnd"
                     /locus_tag="VFU75_04895"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEU5466203.1"
                     /translation="MQLGMIGLGRMGANMVRRLMRDGHECVVHDTSPDAVRALSTEGA
                     IGSSGLDDFVSRLAVPRTVWLMVPAGAVDATLDALLPKLTADDVVVDGGNSYYRDDRS
                     RAQRLAARGLHHVDCGTSGGIVGLERGYCLMIGGEPEIVRRLDPIFVTLAPGVSSAAR
                     TPGREGRPGTAELGYLHCGPNGAGHFVKMVHNGIEYGLMAAYAEGLNILKHAGVGKDA
                     KDARPPDAETTPLRDPDAYQYDLDLANIAEVWRRGSVIASWLLDLTAAALRADPGLDQ
                     FQGRVSDSGEGRWTLEAAIDEAVPANVLAAALFGRFTSRGDADFQNRLLSAMRAQFGG
                     HAERNAASPGRGGPGG"
     gene            complement(12165..>12882)
                     /locus_tag="VFU75_04900"
     CDS             complement(12165..>12882)
                     /locus_tag="VFU75_04900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466204.1"
                     /translation="EWGAWSERRAGGWILQVPTIDILLFNRVIGAGLPAATREEIDEV
                     IQPFKSTKLKSWGVQLSPAAEPDELADWLEEGGLVRRDNWSKLYRPAGEVPEVATGLR
                     IEKAARKDAVAVGRITRTAFGMPEARESWIASIVGRPNGHHYLAVEGGEPVAAAALYV
                     HGTVGWMGVAGTLEGARGRGAQSALLARRLVDGAHLGCQWFVAETGEDLPDRPNSSYH
                     NMVRLGFQLAYQRPNYLPVA"
BASE COUNT         2083 a         4668 c         4245 g         1886 t
ORIGIN      
        1 tcggaacact gcatctgatt cggagtgagg gccgccaaga tgcgccgctg atctgccgtc
       61 gcgggtgcct ccacccgctc gtagctggat tcgcccaatt cccgggccag gtccccgtag
      121 atctcccccg ggtcgcggcc cgcccgcgcc gtgatctcgg cggccagcaa ggccgccacg
      181 atgccgtcct tgtcggtcgt ccacacggtt ccatcccgcc ggagaaaggt cgctccggca
      241 ctttcttcgc cgcagaaccc cagtgacccg tcactcagac ccgcgacgaa ccacttgaac
      301 ccgacgggca cttcgtacag ccggcgcccc acccggtcgc atacccggtc gatcattgca
      361 ctcgacacca cggtcttccc cactccggtg ccctgacccc aatccggtcg atgccggagc
      421 aggtggtcga tggcgaccgc gaggtagtga ttggacggca ggaggcccgt gctccgggcg
      481 accacgccgt gccggtcgtg atcggtgtcg cacgccacgg caatgtcgta gtggtccttc
      541 aggccgatca gccgctgcat ggcataggac gatgacggat ccatccgtat cctcccgtcc
      601 caatcgaccg acatgaatcg gaaggtctgg tccactgcgt cattgacaac cttcaggttg
      661 agccgatagt gctccgcgat ggcaccccag tagtgtactc ccgctccacc gagggggtcg
      721 acgcccaccc ggataccggc ctcgcgaacc aggtcgagat ccacgatctg accgaggtcg
      781 ttcacatagg cagtccggta atcgtaccgg tgaatggtcg gggcgcggag cgcgcgctcg
      841 tatggcatgc gcctcacgcc cttcagtccc tgggccatga tctcgttggc cccggcttcg
      901 atcctcccgg tcacgtcggt gtccgcgggg cccccgtgag gagggttgta cttgaatccc
      961 ccgttgtccg gcgggttgtg ggagggcgtg atcacgacgc cgtcggcaag tccctcggcc
     1021 cgaccgcggt tatagccgat gatggcatgt gagacggcgg gggtcggcgt gtactcgtcg
     1081 tcctcggcca gcatgacgtc cacgccgtgt gccgccaaca cctcgagcgc ggtcgcgcac
     1141 gccggcacgg acagcgcgtg ggtgtcgatt cccagaaaga gcggaccatc gattccctga
     1201 ctcgtgcgat agcggcatag cgcttcgctg atcgcgagga tgtgccactc attgaaggtg
     1261 gtctcgaagg cggagccacg atggcccgac gtgccgaaaa cgacgcgctg cgcaggaacc
     1321 gcgggatcgg ggatccgggt gtagtaggcg gtcaccagct tggagatgtc gaggagcagc
     1381 tcaggagggg cgggatgacc cgccagcgga ctgacgttca caggtgctcc agtcccgcgg
     1441 ctgccgcccg atcgaccagc caatccaccc ggcctccgga cggcgcgata gcttgcgccg
     1501 ggagctcatc ggggcgccgg ggaccttgga gcacgcgctt gagaatttcc gccttatgag
     1561 ctcccgacac gaggaagagc acttccgcgg tggtgttgat caccacgggc gtgagcgtga
     1621 cccgccaggt cgcagtcgca gcctggtact caggcatgac ccatcgagtc cgctcccggg
     1681 cggcggcact ccccggaaag agggatgcgg tatgtccgtc ctcaccaagg cccaggacca
     1741 cgagatcggg tggcccgacc gcccgcacca gatcgcggag agtcgcttca taggcgcgcg
     1801 ccgccaccgc tgggtcatcc tcgccgtgga tgcgatacag gttggctgga gggatcggca
     1861 cgcggtcgag ccaggcggtg cgggccatgc ggtagttgct ctcgacatca tcggggggca
     1921 cacaacgttc gtctccccac accacgtgga cgcatggcca ggggatgcgc gaggcgaacc
     1981 cctctccccc cagtcgctca taggttggtc gcggcgtcga tccgcccgcg aggaccacta
     2041 cggcttgccc acgtccctga atcgcggcgg tcaggtgctg cgtcacacgt tccgcggccg
     2101 catcggccag cgcctcgacg tccgcgagca ccgtgacccc gggccgtatg ccccccccgt
     2161 cgctcactcc gccaacgcct cgaccggacc gccatccgcg gggccacccg gaatccgcgg
     2221 agcgccgctg gcgcccatca cgacccggac ccggtgaacc cccttgccac cgacgagggg
     2281 aacgccgaca cggtgatcga gcgcgacgcc gtcgagctcg gccagtgcca ccccgcggct
     2341 cacgccgtcg ggattctcca cggcgatctc ataacgcgct tccccgtgcc gcaatatcgc
     2401 ggtgaacccg gaccacccag agggaatgca gggatcgacc agaacgtgcc cgctccgaac
     2461 gcggagcccc aacagccatt cgatcccggc ccgatagagc caccccgctg atccggagta
     2521 ccaggtccac ccgccgcggc ctacgtgtgg cggctcggag tagacgtctc ccacggcgac
     2581 gtagggctcg acccggtacc gcaggacacc cgcggcggtg gcggtatggg aaatcggatt
     2641 cagcatcgcg agaagctccg cggccttggc cccgtcaccc agttgggcga aggcgatgac
     2701 cgcccaggca gccgcgtggg tgtattgtcc gccgttctcg cggacgccgg gcacgtatcc
     2761 cttgatgtag cccggctcga gtgcggtgcg gtcgaagggc ggcgtcagga gcacgatcag
     2821 gtcatcggac cgccgcacca ggcgctcgtc caccgacgcc atggcgcgac gtgcccgctc
     2881 cgccccggcc ccgcctgaca gcacgctcca ggattgcgcg atcgagtcga tagcgcaggc
     2941 atccgcacct gcgcccccca gtggtgtccc gtcatcgaag taggcccgcc ggtaccagtt
     3001 cccatcccag gcctcccgct ccaccgcctg cctgagggcg acaccgtggt ccccccacgc
     3061 cgcggcccgg gccgcttccc gccgggtcgc cgcgatcggc gcccaggtcc ggagcacgga
     3121 gtggagaaac caggcgagcc agacactctc tccccgtccc tggctgccca ctcgattcat
     3181 cccatcgttc cagtccccgg tgcccatgag cggaagtcca tgcgcgccga cgcccaggct
     3241 ccgctcgaga gcgcgagcgc aatgctcgaa gagcgtgcca tgttccgccg ccggccgagg
     3301 ctcgaagtac gactccgact gccccgcggc gagcagggcc ccgccgagga acggcacgac
     3361 ctcgtcgaga atggcctggt cacctgtgac ttcgagatag tagccgacga cgtacggcag
     3421 ccagagaagg tcgtcggaga tgcgcgtgcg cacaccgcgc cccgccggtt catgccacca
     3481 atgctggacg tccccttcga cgaactgccg cgatgcggcc ttcagaatgt gcgcacgcat
     3541 cagatcgggc cgcgcgaccg ccagcgccat gacgtcctgg agctgatccc ggaatccgta
     3601 cgctccactc gcctggtaga acgccgtgcg ggcccagagc cggcaggcga gggtctggta
     3661 caggagccac tgattgagca tcaggtcgag agcgcggtcg ggcgtagtca cctggatggt
     3721 ttccagtatg gcgcgccagc caccgctgac cgtcgcaagc cgccggtcga gatcatcgga
     3781 ccggtagcgc tggatcagcg cctgcgcctg ctctcgggtg ccggtctggc ccagcagcca
     3841 caccaccgag acgctctcgc cggccggaat ctcgagggtg acctgcagcg cgcatcccgg
     3901 gtccagcgcc gcgccggttc gcccggacaa cggttggcgg cgaatcaggg ccgcagggcg
     3961 gtcgagtgcg ccattccggc ccaggaattc cgtccggtcc ccggtcgcgg cgacctgacc
     4021 cccgccgaga tccgcgaagg cgatccgcga ggcgaagtcg cggctccacg aattccgcgc
     4081 gaacatcgcc cctgtcgcag ggtccagttc cgtcatgatg aacggcgcgg acgcgctgcg
     4141 cgacgttccc agcacccagt cgtggtatgc cgtcaccgag agatgccgcg gctgcggaga
     4201 atgattggtg agcgtgagcc gggagatctt gaccgggtcg tccgccggta cgaattggag
     4261 caggtcactg gtgacgtcat gcccgtcgta ccggaatcgt gtgtacccag gaccatgccg
     4321 caccacatac tcaccgcccg gctcgcgaat cggtaacggc gtggggctcc acagcgcgcc
     4381 ggtctcgtcg tcacggatgt agaacatgtc gctcggcggg ttccgcaccg gatcgttgga
     4441 ccagggagtc aacaagttct cctggctgtt gatcgaccag gtgcatccgg acccgctctc
     4501 ggatacctgg catccgaagt ccgggttggc gaggacattg atccaggggg ccggcgtcca
     4561 ttgtcctgcc cgcaaggtga tgacgtactc acgggcctcg gcgtcgaatc cgcccaggcc
     4621 gttgaagtac tcgagccggg gagacggcat cgccggcgcc gcgaccaccg gcacctccgc
     4681 cgcctcccgc cgggtcacca cggtcggcgc cggatcggcg cggtgcgcgc gggcgacctg
     4741 ctccacgaga gtgccccgcc ggctggaaag gtcgatgcgg gccacgctct ccagtacgtc
     4801 gcgctgtccg gcggtgacgc ggtccgcccg caggatgatc actcgcccct gagggtcggg
     4861 cccttcgggg cgcggcatgg attggttggt ccgtaccagc gtttcgatga gtccctgcag
     4921 atcctgttca taggacggcg cccggtcgtt gaggatgacc aggtcgaccg cgagtccttt
     4981 caggcgccag tatgcgtgcg cgcgcagtag ctgccgcacg atcccgatgt catccgactc
     5041 gtcgatttcg acgaggacga tgggcagatc gcccgaaatg ccgtgagtcc agagggcggt
     5101 gacaccctca ccgcgccggg cttgcacctc ggtcgacccc cgcagcgcgc gatccgtgta
     5161 gaggagcgcc gagcccagcg tctgaaacaa gtgcgcctcg tcgggcccga tgccgaggtg
     5221 gtgcagctgg acctgggcct gcgtccaggc cagggtggcg acccgctcga aggtcgtgac
     5281 gtcatgatat ttgtcggcca gatcgagtac cgccgcccgc gacggcccca ccagggtcgt
     5341 gaaggctacg cggacggttc tccccggacg cactcgaacc cgccggcgga gactgacgat
     5401 cgggtcgagc acggaaccga ccgtgttcga gaggtggcct ccctccgtct ccgcccgggg
     5461 cgcccgcagc gtcccccccc gccccaggaa ctgcgcgcga tccgtttccc attcgagatc
     5521 accgacggtc tcgccctcca ccgccagcac gtgcgcgagc cacacctcgg catcctccgg
     5581 tgcccggcgc cgccgggtgg ccagcagggt gtcgcggtct gccacgcttt cggtctcgag
     5641 gaacaggttg gagaaggcgg gatgcgcggc gtccgaagcc gccggcgcca ggacgatctc
     5701 cgcgtacgac gtgacgtcga tttcgcggat ccgggcgccg ttgttggtga gggacacccg
     5761 ccggacctcg gcgtcgtcct cggacgacac gatcacttcc aggcgcgtcg tgatcgcccc
     5821 gtcccgccgg ctgaattcgg cccggtcctc ggcgaacgac acgtcgtagc tctcgggagc
     5881 gcttccggtg ggctggaatc cggccgacca gcgatcgtcg ctctcgacat cccgcagcag
     5941 gatatacgaa ccggcatcgt cgcaggtcac gtcctcgcgc caccgcgtaa ccgcgagatc
     6001 ccgccaacgg ctgtaccccg accccgcggc ggtcaccatc accgcgtacc gtccgttcga
     6061 cagaacctgc gtgcgcagcg tggcgccgtc cggcgacgtg aaatgccggg gctggggcgg
     6121 caccagctcg cggacatctc ccaccacacg atcggcctcg gtttggggcc ggaccacggg
     6181 gacgtcgcgc ggggtccgct cctgcaacag gagctccgtg gcgcgtacca tcggctcctg
     6241 gtgaaaccga gcccgcatgg caccaccggt cagcacgttc gcgagcgcga cgaccgacat
     6301 gccctgatgg tgagccatgt acatgcgcac caccgcctgc tccgtctccg ccgggagccg
     6361 gctgggcgtg taatccaccg cttcgtagaa gccgtatcgt ccgtcggcgc cggcccgggc
     6421 gagcgcgcgg aggttccgga gcgctgcttc aggatccacc atcgccgcca gcgcggtggc
     6481 gtagggtgcc acgacgacgt tctccacgag accgcgtttg agccccagcc ccggaacgcc
     6541 gaagctcgag tactgaaacg tcatcgcgag atcgcgcgcg tagtacccgg attcagacat
     6601 gccccagggc acgttacgct cggcgccata ctcgacctgc cgctggacgg ccagccggca
     6661 ggtctgctcg agaagactcc cgagggggga acgcatgatc aatccaggca tcaggtactc
     6721 gaacatcgag cccgaccacg agaggagcac cgagtcccgg cccacgggcg tcaacaaccg
     6781 gctgagccgg aaccagtggc gcaccggcac gtcacccttc gcaatggcga tgaagctcag
     6841 caggcgggcc tcggaggcca gcaggtcata gcgtccgggg tcgagcgtgc ggtcctccat
     6901 ccggaacccg atggcgaaga gcatccgcgt gcggtcgaac acgaacccga agtccatcgc
     6961 ccccaccatg tcgctggcct gagccgcgag ccggttcaga cgttcgatca gcgccgtagc
     7021 ggcggcggcc gaccgctcca gtgacgccgc gagtgcatcc agggcccgca gccggacggg
     7081 agtcccgtcc gcccctgcgg ccacggcggt ccgcaccacg gtgagatcct ccagcgccgc
     7141 ttggcagatc cgcggcagtt cggccagggt cagaggcccc tgcagccgtt ccatcagcgg
     7201 cgccgcccta tcgtccggac ggctggaagc ccacggcatg agtgtgtcga gatcccgttc
     7261 gtgggtttct accgctccct gcagcgcctc ggtggcgctc aacaagggga cgctctgccc
     7321 cgagacgccc ttgcccgtgt cccgggcgcg gttcgcgacg gcttgcgtcg ccgccgccag
     7381 ctccgccaag cgggccgccc aggccgccgg ggtgccggac ggctgaccga ccaccgtcat
     7441 gacgacctcg agggcttccg tgaggcgctc ccaatgcgcc tgcccctccg cttcgtcccc
     7501 gctggaccgc agggcgtgcc gcaagagctc ggcggcatcg gcgatccccg accgcgcgtc
     7561 cgggagcggc accccctgcc ccagcatctc ccggcaggca ttgccaagcg cgatgagcgc
     7621 gcccgccaga ttcccgctgt cgacggtcga gacgtatctc ggctcgagtg ggtgaccgtc
     7681 gcgagtgtcg taccagttgt agagatggcc ccggtactgc tcgaggccct tgatggtccg
     7741 gagcgtcgct tcgatccgat cgaccgcgtc gagcgtcccg agccacccca aatcacgagc
     7801 cgacaccacc gacagcaggt acagcccgat gttggtcggc gacgtgcgct ccgcgatcac
     7861 cggctgcggc gtctcctgga agttgtcggg aggcagatcg cggctctcgg tggtcacgaa
     7921 ggtggtgaag tatccccagg tgcggcgcgc gataaggcgc agggcgcgga cgtcgtccgc
     7981 agacaagggg acggtccgcg gaccggggcc ggggcgggag agccagctca tgatggccgg
     8041 cgcgaccccc cagacgccca gcaggggcag cgccacgggg agggcgccgg gatcgcgcca
     8101 cgcggccgcc gcggcggcga tcgcggtcag gactatgccg ccgctcatcc ggcgatagaa
     8161 cccggcgacc gtggtcggcg cgtgtgcggc cgtcagcgtc atcgtcctcc actcgagcaa
     8221 ccgcgcgtgc gtcaggcaga cgcgccccaa cgtgcgaagg atggcatccc ccatcagcca
     8281 cgcctgatgc gggagcgtcg tgagcgtgac cgccatttga ctcacggcgg accggaaatc
     8341 acgcaccacc gcgcgcacgt gactgcgctt ggcgattccc ggcgtgcgcg gccacaggcc
     8401 ggcgaagact ggaagcagcg gcggggcggc gatgatggcc aggatgaagg ccgtccagag
     8461 caccggcgaa ggacccggca agagccatcc cgccaccagc gtcaggaagg ccgcggggag
     8521 ggacaacgtg cgccggagat tgtcgatcat cttccaccgc cctaccggcg acaggcgctc
     8581 gcgcacgatc cagggcagca gctgccagtc cccgcgtgcc caccggtgct ggcgcgccgc
     8641 cgcggcgaga tagttggcgg gagggctttc gagcaattcg acgtcggtca cgagaccggc
     8701 ccgggcgaac agcccctcga acaagtcgtg gctcaacagg gtgttgtcgg gcacgcgccc
     8761 ctcgagtgcg gcctcgaagg catcgacgtc gtagatgccc ttgcccgtgt acgacccctc
     8821 cccgaagaga tcctgataga catcggagac cgccgccgca tagggatcga tccctgccgg
     8881 gccagagctc aaccgctgga agcgggagcc cccgcgcccc gggagcggcg gagtcacccg
     8941 cggctgcagg atggcgtatc cctcgaccac gcgccgagtt gctccgtcga ggcggggccg
     9001 gttgagcggg tgggccatgg tgccgaccag ccgctgcacc gctccgatcg gcactctcgt
     9061 atcggcgtcg agcgtgatga cgtagcgaac acccgccggc gcggccggca tctccccgtc
     9121 cgcggcgatg aaggtcgtgt cgcgggcacc gcgcagcaga cggttcagct catgaagctt
     9181 cccgcgcttc cgctcccatc ccatccaggc gtgctgcttc gcgttccaga gccgccgccg
     9241 gtgaatcaca aagaagcggt caccgccgcc gggggccggc ccgtggcggg cgttcagccg
     9301 cgccacgccg gtccgcgccg cggacagggt ctcctcgtct ccggcggcat gctcggtgcc
     9361 tgcgtcgagc cagtccgaga ccagggcaaa ccgcacgtcg ccatcggagt tcgccaggta
     9421 gtggacctcg agacgctcaa cctgctcctc cgcatcggcc gcatcggtca gcagcgtggg
     9481 caccgcgacc agcgtccggc agtgctcggg cacgcccgcg gcccactcaa ggcgcggcag
     9541 cggacggggc gccaccacgg tgttgacgaa gcgattcacg agcgcgaccg cgagatcgga
     9601 ggccggaatg agacccagaa caccgaggac cagcagcacc catgagggcg cggcggccca
     9661 ggccgtcagc acgagcggga cggcgaggat ggccaccgtg acaaggagga gcgtgccgag
     9721 atagccggga gtggccgcgt cgatgaaggc ccgccgcagc cggagcggcc acggaggacg
     9781 gaagtgcacg acacgctcga actctcgccg cccacttccg acgagatagt acccggggtc
     9841 cccgccccgc tcatcgagcg cccccccgcg gccggcggac ccgcttcgcg accgggccgc
     9901 cgccgcaagc gccgcctgcg tcacctcgag ctccgtgcgc ccggagccac gcgccagcgc
     9961 ttcgatggcg tggcgatagc tgtcgcgacc ggtgaagtcc atggcggcga aggcgctgcc
    10021 ggcccgcagg gcctcatcga ccaggctcac gctctcgaag aatgcggtcc agtcgagcgc
    10081 cgacatgagc cgcatgctgg tgatgatgtt ccggaccgtg accgtcatcg cggcttgacg
    10141 ctgatgctcg acgagcgcga tttcctccgc actggtgccc tgggccgcca ggcgctggcg
    10201 gagccaggtc agcgccggtg tcaccgcggg gtcttgatcc cgcagtcgct gaaccaactc
    10261 gaccgcgaat gccgtgacca agcgcggact gtcgtcccgc gacagcacgg cggccacgga
    10321 ctgcagcccc tgccctcgcc tgtcgagcag ctcatcggcg agcgcgtcgg cctcccgccg
    10381 cgccgcccgc ccccgaacca tcgcgtccgc cacgcgccgg aggttctcca ccagcacgac
    10441 gcgcagcgag atcgtgaggg cccagagctc accgatgttg aggggttgga cccgctgata
    10501 ggccttgacg aatcgccgca gggtttcggg atcgaagcgg ctgtcggtat gcgccacaaa
    10561 cgcccaggcg atgccgtaca cccggggata cccggtcagg gggccctccg cgagggccgg
    10621 cagctcgcga tagaagccga ccggcagatc gtcccggatc tcccggagct gctcgtcgac
    10681 cacgaagaaa ttgtccacca accactcggc cgccggcgtg atccagtgtt cttcgcgcac
    10741 ggcctcggcg atggtgcggt tcgcctcgcg caacacccgg ccgttgtcgt cgacacgtgg
    10801 gaggaggggt ttctggggtt cgcggccagc cgccaccggc tgcgccgccg ccaagctctc
    10861 cgcgtgctgc tccaggcgtt cgatgccgaa cagctcggcc cggatcgggt cctcgagtgg
    10921 cgggagggac gggaagaaga tcggcagagg aacccgcatc agtcagccgc tcccgggggc
    10981 cagagggcgc tcgtcaccca cccggcccgc cccggcccgg cgaggcggcg ttccgttccg
    11041 cgtgcccgcc gaattgcgcc cgcatcgcgg agagcagacg attctggaag tccgcatcgc
    11101 cccgcgacgt gaagcggccg aagagcgccg cggccagcac gttggcgggc accgcttcgt
    11161 cgatcgccgc ctcgagggtc caacgtccct cccccgagtc ggagactcgg ccctggaact
    11221 ggtccagccc cgggtcggca cgcagtgccg ccgcggtcag gtcgaggagc cacgacgcga
    11281 tcacactgcc gcgccgccac acctccgcga tgtttgcgag atcgaggtcg tattgatagg
    11341 cgtcgggatc gcgaaggggc gtcgtctcgg catccggggg ccgtgcatcc ttcgcgtcct
    11401 tccccacgcc ggcgtgcttc aggatattga gtccctcggc atacgccgcc atcaacccat
    11461 attcgatgcc gttgtgcacc atcttcacga agtggccggc tccgttcggc ccgcagtgga
    11521 gatagcccag ttcagcggtt cccggccgcc cctcacgccc gggcgtcctc gcagcggagc
    11581 tcactcccgg cgccagcgtg acgaagatcg gatccagccg ccgcacaatc tccggctcgc
    11641 caccgatcat caggcagtag ccgcgctcca gcccgacgat ccccccactg gtgccgcagt
    11701 ccacgtgatg gaggccccgt gcggccaggc gttgcgcgcg ggagcggtca tcccggtagt
    11761 aggagttccc tccgtccacc acgacgtcat cggcagtcag tttagggagg agagcatcga
    11821 gggtggcatc gacggcgccg gcggggacca tcaaccacac ggttcggggt acggccaggc
    11881 gggagacgaa gtcgtcgagc ccgctggacc ctatcgctcc ttcggtggag agggcccgca
    11941 ccgcgtcggg tgacgtgtca tgtacgacgc actcatgccc atcgcgcatg agacggcgca
    12001 ccatgttggc gcccatgcgc ccgagtccga tcatccccag ctgcactgtc gtcctcccga
    12061 gaagattccc ctcctctcaa gatcgacagt accaggcccg ggggggaccc cccagttggg
    12121 atgagttccg gggtgaaccc gacgcccgtc cgcgagtgcc ggtgtcacgc gaccggcaag
    12181 tagttcggcc gctgatacgc gagttgaaat cccaatcgca ccatgttgtg atacgaggaa
    12241 ttcggccggt ccgggagatc ctcaccggtc tccgcgacga accactgaca tccgaggtgc
    12301 gccccgtcga ccagccggcg cgccagcaac gcgctctgcg ccccccggcc ccgcgcgccc
    12361 tccaacgtcc ccgccactcc catccagccc acggtaccgt gcacgtacag agccgccgcc
    12421 gccaccggct ccccaccctc caccgccaaa tagtgatgcc cattggggcg ccccacgatc
    12481 gacgcgatcc acgactcgcg cgcctcgggc atcccgaacg ccgtccgcgt gatgcgtccg
    12541 acggcaaccg catccttccg cgccgccttc tcgatccgca gaccggtcgc gacctccggc
    12601 acctcccccg ccggacggta cagcttgctc cagttgtcgc gccgcaccaa cccaccctct
    12661 tcgagccagt ccgccagctc atcgggctcc gccgccggcg aaagctgaac tccccagctc
    12721 ttcagtttgg tcgacttgaa gggctgaatg acctcgtcga tctcctcccg cgtcgccgcg
    12781 ggcaggcccg caccgatcac ccggttgaag agcaggatat caatcgtggg gacctgcaga
    12841 atccagcctc ccgcccgacg ctccgaccac gccccccact cc
//
LOCUS       DASUBP010000131         5898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_221596, whole genome shotgun sequence.
ACCESSION   DASUBP010000131 DASUBP010000000
VERSION     DASUBP010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5898
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221596"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..963
                     /locus_tag="VFU75_04905"
     CDS             <1..963
                     /locus_tag="VFU75_04905"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HEU5466205.1"
                     /translation="IGSDLILDNEDLEATFAPGRNFANKLWNIGRFILSQLPEQVPTI
                     EAIDRADLTLADRWILSRCQSTIEAATKANEQFRLDEAAKLCYEFVWGDLADWYLESA
                     KGRMGTAAYAVLAHCFDIGLRLLHPDVPFITEELWQKLPGRKPGELLAAAAWPVPNAK
                     ARDAEADLAFGRVQELITAIRSIRAEYRIAPKTRLSAVLNSKAADAYAAEIETITRLA
                     GLENLVIGGKAQGVGSHAVLKDGSDVFVGLGGSIDVSQECKRLTTDRDRLDKQLASLS
                     AKLSNEGFVSRAPADVVAKEREKEQAWRAQVGALNDKLKALGCA"
     gene            954..2273
                     /locus_tag="VFU75_04910"
     CDS             954..2273
                     /locus_tag="VFU75_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU5466206.1"
                     /translation="MRLTPLAALGVVATACASMGQPPGAPPRLTPPAIILVSPDSGAV
                     LPTFSGDAVIQFDEVIDEMAQGTGGAPGTVTGLAKQIVLSPVAGPVKVSWHRSAIHIK
                     PKEGWKPGRVYHLQLLPGIVDLHRNILKRGETIIFSTGPALPTASLRGIALSWVEQHT
                     IPQALIRAVLKPDTIAYLTYTDSTGRFRLDGIPPGQYVVYAVNDQNSNRARDQREAFD
                     SDLVTLDSTAAATLWTFVHDTTGPRLRAPEPLDSSSFRLTFTAPLSPAHVPDTGTVHL
                     YELPDTTPVALSAVLTPAANDSLVARQRAVADSLRRAADTTRAAVDTTKKKAAAGRDT
                     TARKPPTQGPVSHPGAASAVDSAVIKLLATRPIPTDKIVVRTSMPLKPSTKYFIRVTG
                     ATNLNGVRADAVSVLVVPAPKKVAADSTAKAKADTTAKAKPDSTRHP"
     gene            2270..3625
                     /gene="selA"
                     /locus_tag="VFU75_04915"
     CDS             2270..3625
                     /gene="selA"
                     /locus_tag="VFU75_04915"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNA(Sec) selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HEU5466207.1"
                     /translation="MTDPRRKLPSVDAMLAQPGIAALLPGHPRPLVVNAIRTVLDGAR
                     AVGGTEPSEGWSAAVASQLTRVAAPSFQPVINATGVVLHTNLGRAPLAPAAIEAMTRI
                     AAGYSTLEYDVGAGARGSRHLHCRDLLVELTGAEDALVVNNAAGGLLLALSALGRDGE
                     AIVSRGELVEIGGAFRVPDIMARSGAKLVEVGTTNRTHLDDYAKAVSHKTRVLLKVHR
                     SNFKVSGFTADVSPNEVSQLAHASGAAALFDLGSGLLMDLAPWHLSGEPTVKEAVASG
                     VDLVVFSGDKLLGGPQAGILVGTRAVIEACRKDPTARAVRADKLTLAALEATLRLYRE
                     PETARRDIPVLRMLTKSLDEIRREGKSLAKDIGAEASLISGESEVGGGSFPGTTLKTW
                     LVRIAVSHLSADGLSDRLRAGSPPVITRIADGHVVLDPRTLSPGEIPLTARAVRSALD
                     A"
     gene            3618..4178
                     /locus_tag="VFU75_04920"
     CDS             3618..4178
                     /locus_tag="VFU75_04920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEU5466208.1"
                     /translation="MPEPGRRRSAAFLDRDGTLIEDPGFLSDPAKLKLLPGAAQAVRR
                     LNQGGLAVVIVTNQSGIARGLITAQQYDAVHARLVQLLAGAGARLDGAYVCPHHPDIS
                     GPCECRKPGTKLFRDAATELTLDLGRSFYVGDRMSDVEPARALGGRGILVRTGRGGEY
                     AEGAINAGYTVVADLQTAVDLILEHQ"
     gene            4175..4618
                     /locus_tag="VFU75_04925"
     CDS             4175..4618
                     /locus_tag="VFU75_04925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466209.1"
                     /translation="MTRIFVALCLALVAATPARAQSDTTHQIVVGATVTGALTRNDRR
                     TADGTYAQAWTLKATPGHVVTIDLASKAFDAFLMARGAGLDSTMGPLQDDDSGGHCNA
                     RLTLRMPASGECTIVVTTSNRNTVGDFTLAVRSGAIPASLAPCHG"
     gene            4615..5223
                     /gene="purN"
                     /locus_tag="VFU75_04930"
     CDS             4615..5223
                     /gene="purN"
                     /locus_tag="VFU75_04930"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEU5466210.1"
                     /translation="MTVTPVRVAVLASGGGTNLQALLDGLNGAQNKAARVSRVISNRS
                     DAGALDRGRQAGVPTYALSDPTNADEYLEAIGDAQLVVLAGYLKLVPSAVVSRFDWRM
                     INIHPALLPEFGGPGMYGLKVHQAVLAAGKKESGASVHYVTEEYDRGEVIARARVPVL
                     PGDTAERLAARVLTAEHRLLPHVVTVMSLMGLPRHAVRLEDL"
     gene            5223..>5898
                     /locus_tag="VFU75_04935"
     CDS             5223..>5898
                     /locus_tag="VFU75_04935"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466211.1"
                     /translation="MRTRCAAVILLLATPLSLPSQSLESLTLRFAGFTTVSGYEQAVT
                     DSLLTLFPGATRDRLGDVSITLGTGSPRRLVSCPLDEAGFVVGGITSEGYLTLRREGR
                     VASPLFEQQLEGHRVTVFGASGPVPGVVGVRSVHLTRGRVPNETPFTVDDAYVDIGAS
                     SAADAAALGVRLLSPVALSRRPHRYGEGMAAPAAGRHAACAALASALLSHPKVTGSVV
                     VSFTVQG"
BASE COUNT          951 a         2112 c         1903 g          932 t
ORIGIN      
        1 atcgggtcgg atctgatcct cgacaacgag gacctggagg ccaccttcgc gcccgggcgc
       61 aacttcgcga acaagctctg gaacatcggg cgcttcattc tctcccagct gcccgagcag
      121 gtgcccacga tcgaggcgat cgatcgggcc gatctcacac tcgcggaccg gtggattctc
      181 tcgcgctgcc aatcgacgat tgaagcagcc accaaagcca acgagcagtt ccggctcgat
      241 gaggccgcca agctgtgcta cgagttcgtg tggggagacc tcgccgactg gtatctggag
      301 tcggccaagg gacgcatggg gaccgcggcc tacgcggtac tcgcgcactg cttcgacatc
      361 gggctccggc tcctccaccc ggatgtccct ttcattacgg aagagctctg gcagaagctg
      421 ccaggacgca agcccggaga gctcctggcc gccgccgcat ggccggtgcc caatgccaag
      481 gcgcgagacg ccgaggcgga cctggcgttc ggccgggtgc aggagctgat cacggcgatt
      541 cgctcgatcc gcgccgaata ccggatcgcg ccgaagacca ggctctcagc ggtattgaac
      601 agcaaggctg ccgacgcgta tgccgccgaa atcgaaacca tcacccggct cgccggactg
      661 gaaaacctcg tcatcggtgg gaaggcacag ggagtcggct cccatgccgt gctgaaggac
      721 ggcagcgacg tgttcgtcgg cctcggcggc tcgatcgacg tctcccagga gtgcaagcgg
      781 ctcactacgg accgcgaccg gctcgacaaa cagctcgcca gcctgtcggc caaattgtcg
      841 aacgagggat tcgtgtcacg tgcgcccgcg gacgtggtgg ccaaggaacg cgagaaggag
      901 caggcctggc gcgcccaggt gggagccctg aacgacaagc tcaaggcgct cgggtgcgcc
      961 tgacccccct cgccgccctg ggcgtcgtcg cgacggcctg cgcgagcatg ggccaacccc
     1021 ccggtgctcc gccacgtctc actccacccg ccatcatcct cgtttcaccg gactcgggcg
     1081 ccgtcctccc cacgttttcg ggagacgcgg tgatccaatt cgatgaagtg atcgacgaaa
     1141 tggctcaggg aacgggcggc gcgccgggaa cggtgacggg gctcgccaag cagatcgtgc
     1201 tttcgccggt cgccggaccc gtcaaagtct cgtggcaccg ctcggcgatc cacatcaagc
     1261 cgaaggaagg atggaagccg gggcgcgtgt atcacctgca gctcctgccc ggcatcgtgg
     1321 acttgcaccg caatatcctg aagcgcggcg agacgatcat cttctccacg ggacccgccc
     1381 tgccgaccgc ttccttgcgt ggcatcgctc tgtcgtgggt cgagcagcac accatcccgc
     1441 aggcgctgat ccgcgcggtc ctgaagccgg acaccatcgc gtacctcacg tacaccgact
     1501 cgacgggccg cttccgcctc gacggcatcc ctcccggaca gtacgtcgtc tatgcggtca
     1561 acgatcagaa ctcgaaccgc gcgcgagatc agcgcgaggc cttcgactcg gacctggtga
     1621 ccctcgacag caccgcggcg gccacgctct ggaccttcgt ccacgacacc acggggcccc
     1681 ggttgcgcgc gcccgaaccc ctcgattcgt catcgttccg gctcaccttc accgccccgc
     1741 tttccccggc tcacgtgccc gacaccggca ccgtgcacct ctacgagctc ccggacacca
     1801 caccggtcgc cctatccgcg gttctgactc cagccgccaa cgactcgctg gtcgcgcggc
     1861 aacgcgccgt cgccgactcg ctccggcgcg ccgcggatac cacgcgcgcg gcggtggaca
     1921 ccacgaagaa aaaagcggcg gcgggccgcg ataccaccgc ccggaagccc ccgacccagg
     1981 gtcccgtcag ccatccgggg gccgcctcag ccgtcgacag cgccgtcatc aagctcctgg
     2041 ccacgcgccc cattcccacc gacaagatcg tggtccggac cagcatgccc ttgaagccgt
     2101 cgacgaagta cttcatccgc gtgacgggcg ccacgaacct gaacggcgtc cgtgccgatg
     2161 cggtgagcgt cctcgtggtc cccgccccca agaaggtcgc ggcggacagc actgcgaagg
     2221 ccaaggccga caccacggcc aaggccaagc cggactccac caggcatcca tgaccgatcc
     2281 gcgccggaag cttccctcgg tcgacgcgat gctggcgcaa ccaggcatcg ccgcgctcct
     2341 gccgggccat ccgcgcccgc tggtggtcaa cgccatccgg acggtactcg acggcgcccg
     2401 ggccgtcgga ggcactgagc cgagtgaggg atggagcgcc gccgtggcaa gccagctgac
     2461 tcgcgtggcg gccccttcct tccaaccggt catcaacgcg accggtgtcg tgctgcacac
     2521 caacctggga cgcgcccccc tcgctcctgc cgccatcgaa gcgatgacga gaatcgcggc
     2581 gggctattcg acgctcgagt acgacgtcgg cgcgggcgcg cgcggctcgc ggcatctcca
     2641 ctgccgggac ctgctggtcg agttgaccgg cgcggaggac gcgctggtgg tgaacaacgc
     2701 cgcgggtggg ttgctcctcg cactgtcggc tctggggcgg gacggcgagg cgatcgtctc
     2761 ccggggcgag ctcgtcgaga tcggcggcgc gttccgcgtc ccggacatca tggcgcgctc
     2821 cggcgcgaaa ctggtcgagg ttggcaccac caaccgcact cacctcgacg actacgccaa
     2881 ggccgtgagc cacaagacgc gggtgctcct caaggtgcac cggtcgaact tcaaggtgtc
     2941 cggcttcacc gcggacgtct ctcccaacga ggtctcacag ctcgcgcacg caagcggtgc
     3001 ggcggcgctc ttcgacctgg gctccggact cctgatggac ctcgcaccct ggcacctctc
     3061 cggtgagccg acggtgaagg aggcggtggc gagtggcgtc gacctcgtgg tattcagcgg
     3121 tgacaagctc ctcggcggac cgcaggccgg catcctcgtc gggacgcgcg cggtgatcga
     3181 ggcgtgccgg aaggacccca ccgcccgggc cgtccgcgcc gacaagctta cgctcgccgc
     3241 gctcgaagcc accctgcgcc tgtaccggga gccggagacc gcgcggcgtg acattccggt
     3301 actgcgcatg ctgacgaaga gcctggacga gatcagacgc gagggcaagt ccctggcgaa
     3361 ggacatcggc gccgaggcca gcctgatctc cggggagtcc gaggtgggcg gcgggtcgtt
     3421 ccccggcacg accctcaaga cctggctggt gcggatcgcg gtcagccacc tctccgccga
     3481 cggtctctcg gatcggctcc gtgccggatc tcctccggtg atcacgcgca ttgcggatgg
     3541 ccatgtcgtc ctcgacccgc gcaccctctc ccccggcgag atccccctga ccgcccgcgc
     3601 ggtgcgctcg gcactggatg cctgagccgg gccggagacg cagtgccgcc tttctcgatc
     3661 gggacggcac cctgatcgag gaccccgggt tcctctccga tcccgccaaa ctgaagttgc
     3721 ttcccggagc ggcgcaggca gtccggcgcc tcaaccaggg aggactggcg gtggtgatcg
     3781 tgaccaatca atcagggatc gcgcggggac tgattaccgc acagcaatac gacgcggtcc
     3841 atgcccgcct ggtgcagctc ctcgccggag ccggcgcccg tctcgacggc gcctacgtct
     3901 gcccccatca tcctgacatt tccgggccct gtgagtgccg gaagcccggt accaaactct
     3961 tccgggacgc cgccaccgag ctcacgctgg acctcggccg ttccttttat gtaggagacc
     4021 ggatgtccga cgtcgaaccg gcccgcgcgc tcggtggtcg tggcatcctc gtcaggaccg
     4081 gtcgtggcgg cgagtacgcg gagggagcga tcaacgcggg gtataccgtg gtggcggatc
     4141 tccaaacagc ggtcgacctg atcctcgagc accaatgacc cgcatcttcg tggccctttg
     4201 tctggcgctc gtcgcggcca cacccgcgcg cgcccagtcc gatacgacgc accagatcgt
     4261 cgtcggcgcc accgtgacgg gtgcgctcac ccggaacgac cgccggaccg cggacggcac
     4321 ctatgcgcaa gcgtggacct tgaaggccac gcccggtcac gtggtgacga tcgatctcgc
     4381 ctcgaaggca tttgatgcct ttctcatggc gcggggcgcc gggctcgact ccaccatggg
     4441 cccgctccag gatgacgact ccggtggcca ctgcaacgcc cgtctgaccc tccgcatgcc
     4501 ggccagcggc gagtgcacca tcgtggtgac gacttcgaac cggaatacgg ttggcgattt
     4561 cacgctggcg gtccgctcgg gcgcgatccc ggcgtccctc gccccctgtc acggatgacc
     4621 gttacccccg tgcgggtggc ggtgctggca tctggcggag ggaccaacct ccaggccctg
     4681 ctcgatggcc tgaatggcgc gcagaacaag gccgctcgcg tgagccgggt gatttccaac
     4741 cggagtgacg cgggcgcatt ggatcggggg cgccaggccg gcgtgccgac ctacgccctg
     4801 tcggatccca cgaatgccga cgaatacctc gaagccatcg gggacgccca gctcgtggtc
     4861 ctggccgggt atctcaaatt ggtccccagc gccgtcgtgt cccggttcga ctggcggatg
     4921 attaacatcc accccgccct gctaccggag ttcggtggtc ccggcatgta cggcctcaaa
     4981 gtgcaccagg ccgtcctggc cgcgggcaag aaggaatcgg gagcaagtgt ccactacgtg
     5041 acggaggagt acgaccgggg cgaggtcatc gcgcgcgccc gcgtgcccgt ccttcccggt
     5101 gataccgccg agaggctggc ggctcgagtg ttgaccgccg agcaccggtt gctcccccac
     5161 gtggtcaccg tgatgtcact gatgggattg ccacgccacg ccgtccggct ggaggatctt
     5221 tgatgcgtac ccgttgcgcc gccgtcatcc tgttgctcgc gacacccttg tccctgccat
     5281 cgcagtcgct cgaatccctc acgctccgct ttgccgggtt caccaccgtt tcggggtacg
     5341 agcaggcggt gacggattcg ttgctgacgc tcttccccgg tgcgactcgg gaccggttgg
     5401 gtgacgtcag catcaccctg ggaaccgggt cgccgaggcg gctcgtctcc tgtccactgg
     5461 atgaagcagg atttgtggtg ggtgggataa cgagcgaagg gtatctcacc cttcgccgcg
     5521 aagggcgcgt cgcgagtccc ctcttcgagc aacagctcga aggacaccgc gtcacggtgt
     5581 tcggcgcgtc cggaccggtc cccggtgtcg taggggtacg atcggtccat ctgacccgtg
     5641 gacgagtccc gaacgagacg cccttcacgg tggacgacgc atacgtcgac atcggagctt
     5701 cgtccgccgc cgatgcggcg gccctcggcg tgcgactgct ctcccccgtg gcactgtccc
     5761 ggcgacccca ccggtatggt gaagggatgg cggctccggc tgccggccgc catgcggcct
     5821 gcgccgcgct cgcctccgcc ctgctctctc accccaaggt cactggatcg gtcgtcgtca
     5881 gcttcaccgt ccagggtc
//
LOCUS       DASUBP010000132         4874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_385138, whole genome shotgun sequence.
ACCESSION   DASUBP010000132 DASUBP010000000
VERSION     DASUBP010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385138"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1215
                     /locus_tag="VFU75_04940"
     CDS             <1..1215
                     /locus_tag="VFU75_04940"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEU5466212.1"
                     /translation="AAGQEIPNGSILFKDGRIVAVGTSVTAPADAVVVDGTGKFVTPG
                     IIDVHSHMGVYAAPGTWAESDGNEATRPNTADVWAEHSFWPQDPQIPLAIAGGVTTIE
                     ALPGSANLIGGRSAVLKLVPSRTVQGMKFPNAPYGLKMACGENPKRVYGDRGGPQTRM
                     GNMAGYREAFIAAEAYRRRWDKWNAKHEGDPPDRDLKLETLAEVLRGHILVENHCYRA
                     DEMAQMMDLGREFGFKIRSFHHAVEAYKIRDLLAANGTGATVWSDWWGFKEEAMDGIH
                     ENAALLDQAGVKINIHSDDAGVIQHMNQEAAKAMYYGIQAGIPVSRDHALRWITANPA
                     WTLGIDSIVGTLEVGKMADVVLWSGDPFSVYSRAQQVYNDGWLVYDRNDPQHQPRTDF
                     ELGQEPLPGSDR"
     gene            1212..2417
                     /locus_tag="VFU75_04945"
     CDS             1212..2417
                     /locus_tag="VFU75_04945"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5466213.1"
                     /translation="MIAVILAQTIALTGGTVYPVSGPKIDHATVVIQNGKITAVGANV
                     TIPGDATRIDVSGKWVTPGLIDGLSNLGLAEIDAVEGTSEGSTGKDIASSFNVSEGLN
                     PASTLIPINRIEGVTSALATPGGGLIGGQAVLIDLDGGTIEQMIDRSPAAMVMDLSEG
                     AKHTGNGSRAGVSARLRQVFNDALEYSRRRTDYNKAQMEALSAPAADLEALLPVLRGE
                     EPVLVNVNRKSDILTALRIAKEYKLKLILGGASEGWEVASEIAAAGVPVLVAPMDNLP
                     SYDGLGTRFGNATMLAKAGVKVGFYFSDSHNARDIRQQAGTAVSFGMSWDQALRAITL
                     TPAEIYGVADRYGSLAAGKVANVVVWSGDPFEFSTAVEHVYIRGREIPMTSRQKELLE
                     RYKTVPPAY"
     gene            complement(2522..2776)
                     /gene="rpmA"
                     /locus_tag="VFU75_04950"
     CDS             complement(2522..2776)
                     /gene="rpmA"
                     /locus_tag="VFU75_04950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEU5466214.1"
                     /translation="MAHKKGVGSTRNGRDSNPQYLGVKRFGGEFVKSGNILVRQRGTK
                     FHPGANVRRASDDTLFAVADGQVKFEHKSKTRYKVSVYPA"
     gene            complement(2784..3101)
                     /gene="rplU"
                     /locus_tag="VFU75_04955"
     CDS             complement(2784..3101)
                     /gene="rplU"
                     /locus_tag="VFU75_04955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEU5466215.1"
                     /translation="MTYAIFKAAGQQFRAEEGRTIKVPLLDAEPGSKVTFDEVLLTSD
                     GKTIQTGTPKVKGAKVTAEVVRHGKARKIRIYRFARRSGYRRHAGHRQQFTEVKIADV
                     KVG"
     gene            complement(3185..4774)
                     /locus_tag="VFU75_04960"
     CDS             complement(3185..4774)
                     /locus_tag="VFU75_04960"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HEU5466216.1"
                     /translation="MAILEDDRLVELLVDRPDSRRSVGDIYLGKVEAVLPGIQAAFLN
                     IGAEKSAFLHASDLIESEDEDEPGEPEDVDDEPVEGDGAGNGNGAPSAPAAQQPQQGG
                     RGRNGGGRRGRRPLPNIQDELKRGQTKLVQVIKEPISTKGPRVTAQISLPGRFLVYMP
                     YASKVGVSRKIENREQRAKLREMVSRLVPKDEGGWIIRTVADDLTEESCKREIDHLVG
                     LWKKIVRKSKSVRAPALVQRETSLTRGIVRDLFSDKVDGLLVDSKQLYNEIEQYLGQI
                     DPELLGRVKLYQQESPLFDEYDIEAEIRGLFAQRVDMPTGGYIIIQPTEALVSIDVNT
                     GRYTGKKDPESTILRTNLEAAREVARQLRLRDIGGIIVVDFIDMETRGNRDKVLHELR
                     THLGRDRARTKAYAVSELGLIEMTRQRVRPSLWQSMTAECPTCSGTGRVFRPEVVVRR
                     MERSLKRAGADHKERQLAVRLHPEVAIYLVEQEPNFLRQLEKQTGLQLEVRDDPMMRL
                     DEFRMMAKPAGRDVTEQYAVA"
BASE COUNT          828 a         1592 c         1592 g          862 t
ORIGIN      
        1 gccgcgggtc aggagatacc gaacggctcg atcctgttca aagacggccg catcgtcgcc
       61 gtgggcacgt ccgtcaccgc acctgcggac gcggtcgtcg ttgacggcac gggcaagttc
      121 gtcacgccgg gcatcatcga cgtgcactcg cacatggggg tgtacgcggc gccgggcacg
      181 tgggccgaga gtgacggcaa tgaagcgacc cgcccgaata ccgccgacgt gtgggccgag
      241 cattcgttct ggccacagga tccgcagatc ccgctggcga tcgccggtgg cgtcaccacc
      301 atcgaggccc tgccgggctc cgccaacctg atcggtggcc ggagtgccgt gctgaagctc
      361 gttccgagcc gcaccgtgca aggaatgaaa ttcccgaacg ccccctacgg cctgaagatg
      421 gcgtgtgggg agaaccccaa gcgggtctac ggcgaccggg ggggccctca gacccggatg
      481 ggcaacatgg ccgggtaccg ggaggccttc atcgcggccg aggcgtatcg gcgccggtgg
      541 gacaaatgga acgcgaagca cgagggcgat ccgccggacc gcgacttgaa gctcgagacg
      601 ctcgccgagg tgctccgggg ccacatcctg gtcgagaacc actgctaccg ggccgacgaa
      661 atggcccaga tgatggactt gggccgggaa ttcggcttca agattcgctc gttccatcac
      721 gccgttgagg cgtacaagat ccgcgacctc ctggccgcca acggtacggg ggccacggtc
      781 tggtccgact ggtgggggtt caaggaagag gcgatggacg gtattcacga gaatgccgcc
      841 ctgctcgatc aggccggcgt gaagatcaac atccactcgg atgacgccgg cgtcatccag
      901 cacatgaacc aggaggcggc caaagccatg tactacggca tccaggccgg catcccggtc
      961 agccgcgatc acgctctccg gtggatcacc gccaacccgg cctggaccct cggcatcgac
     1021 tcgatcgtgg ggacgctcga agtgggcaag atggcggacg tggtgctgtg gtccggcgat
     1081 ccgttctcgg tctacagccg cgcccagcag gtctacaacg acggctggct ggtctacgac
     1141 cgcaacgatc cccagcacca gccgcgcacc gatttcgagc tgggccagga accccttccc
     1201 gggagcgacc gatgatcgcc gtgattctcg cgcagaccat cgccctcacc ggagggaccg
     1261 tctatccggt atcggggccc aagatcgacc acgccaccgt ggtgattcag aacggcaaga
     1321 tcaccgctgt gggtgccaac gtgaccattc ccggcgacgc gacccgcatc gacgtgtccg
     1381 gcaagtgggt gacgccgggc ctcatcgacg gcctctcgaa cctcggcctg gccgagatcg
     1441 acgccgtgga gggcacgagc gagggcagca ccggaaagga catcgcgtcg agcttcaacg
     1501 tctccgaagg tctcaatccc gcctcgacct tgatccccat caaccggatc gagggtgtga
     1561 ccagtgccct ggcgacgccg ggtggtggcc tgatcggcgg acaggcggtc ctgatcgacc
     1621 tcgacggcgg caccatcgaa cagatgatcg accgctcccc cgcggcgatg gtgatggatc
     1681 tctccgaggg ggccaagcac acgggcaatg gctcccgcgc cggcgtgagc gcgcgactgc
     1741 gtcaggtgtt caacgacgcc ctggagtatt cgcgtcgcag gaccgactac aacaaggcac
     1801 agatggaagc gctgtcggcg cccgcggctg acctggaagc gctcctgccg gtgttgcgtg
     1861 gcgaggagcc cgtcctggtc aacgtgaacc ggaagagtga catcctgacg gcgctccgga
     1921 tcgcgaagga gtacaagctc aagctgattc tcggcggcgc gtcggaaggc tgggaagtcg
     1981 cgtccgagat cgcggcggcg ggtgtgccgg tgctggtggc cccgatggac aacctgccga
     2041 gctacgacgg gctggggacg cgcttcggca atgcgacgat gctggcgaag gccggcgtca
     2101 aggtcgggtt ctacttctcc gactcacaca acgcgcggga tatccgccag caggcgggga
     2161 ccgcggtgtc cttcgggatg tcgtgggacc aggccctgcg cgccatcacc ctcacccccg
     2221 ccgagatcta cggtgtggct gaccggtacg ggtcactcgc ggccggcaag gtggcgaacg
     2281 tcgtcgtgtg gtctggggat ccgttcgaat tctcgaccgc cgtggaacac gtctacatcc
     2341 ggggccggga gattccgatg accagcaggc agaaagagct actggagcga tacaagacgg
     2401 taccgcctgc ctactagacg gctagacggt tagacggcta gtgaaaacac gaaaggcggt
     2461 cttccgaggg gaggccgcct ttgtgcgtat ctaaccgtct tgccgtctaa ccgtccagct
     2521 gtcaggccgg atacacactc accttgtatc tcgttttgct cttgtgctcg aacttgacct
     2581 ggccgtccgc gaccgcgaag agcgtgtcgt cggaggcacg ccgcacgttg gcgcccgggt
     2641 ggaacttggt gccgcgctgg cggaccagga tgtttccgga cttcacgaac tcgcccccga
     2701 agcgcttgac gccgaggtat tgcgggttcg aatcgcggcc gtttcgggta gagccgacac
     2761 ccttcttatg agccattagc gcctcacccg accttcacgt cggcgatctt cacttcggtg
     2821 aactgctggc ggtgtccggc gtgacggcgg tatcccgagc ggcgggcgaa ccggtagatc
     2881 cggatcttcc gggctttccc gtggcgcacg acctcggccg tgaccttggc gcccttgacc
     2941 ttgggcgtac cggtctgaat ggtcttgccg tccgacgtca acagcacctc gtcgaacgtc
     3001 accttgctcc cgggctcggc atcgagcagg gggactttga tcgttcggcc ctcttcggcc
     3061 cggaactgct ggccggcggc cttgaaaatg gcatacgtca tagggcgcgg aagctagtga
     3121 gggtcatagg gttagtcaac ctcctgagcg ccattggcgc gaaggatctg ctttgccttg
     3181 gtttttaggc caccgcgtac tgctcggtga cgtcccggcc ggcgggcttg gccatcatcc
     3241 ggaactcatc cagccgcatc atggggtcat cccggacctc gagctggagc cccgtctgct
     3301 tctccaattg ccgcaagaag ttcggctcct gctccaccag gtatatggcc acctcagggt
     3361 gcagacggac ggccagctgg cgctccttgt ggtctgcccc ggcccgcttg agggaccgct
     3421 ccatgcgccg gaccaccact tcgggacgga acacccgccc cgtgccggag cacgtcgggc
     3481 attcggccgt catggactgc cagagcgaag gccggacccg ctgacgggtc atctcgatca
     3541 ggcccagctc cgacaccgcg tacgccttcg tccgtgcgcg atctcgtccc aagtgcgtcc
     3601 ggagctcatg gagcaccttg tcgcggttgc cgcgggtctc catgtcgatg aagtccacca
     3661 cgatgatccc gccgatgtcc cggagccgga gctgccgggc cacttcacgg gccgcttcga
     3721 gattggtccg gagaatggtg ctctcggggt ccttcttgcc cgtgtaccgg cccgtgttga
     3781 cgtcgatcga gaccagcgcc tccgtcggct gaatgatgat gtagccgccc gtcggcatgt
     3841 cgacccgttg ggcgaagagc ccccggatct ccgcttcgat gtcgtactca tcgaacagcg
     3901 gggactcctg ctggtacagc ttcacgcgcc cgagcaactc ggggtcgatc tgacccaggt
     3961 actgctcgat ttcgttgtag agctgcttcg agtcgaccag gagcccgtcg accttgtccg
     4021 agaacaggtc ccgcacgatg ccccgggtga gcgacgtctc ccgctgcacc agcgccgggg
     4081 cccggaccga cttgctctta cggacgatct tcttccagag tcccaccaga tggtcgatct
     4141 cgcgcttgca gctctcctcc gtgaggtcgt cggccaccgt gcggatgatc cagccgccct
     4201 cgtccttggg aacgaggcgg ctcaccatct cgcggagctt cgcccgctgc tcgcggtttt
     4261 cgatcttgcg ggagaccccg accttcgagg cgtacggcat gtagaccaga aaacggcccg
     4321 gcagggagat ctgcgcggtg acgcgcggac ccttggtgct gatcggttcc ttgatgacct
     4381 gaacgagctt ggtctggccc cgcttcagct cgtcctggat gttggggaga ggcctgcggc
     4441 cgcgacggcc accgccattc cggcctcggc caccctgttg cggttgctgg gcggctggag
     4501 cggagggtgc gccgttgcca tttccggccc cgtcgccctc gacgggctcg tcgtccacgt
     4561 cctcgggctc acccggctcg tcctcgtcct ccgactcgat caagtcggaa gcatggagga
     4621 aggcactttt ctcggcgccg atattcagga acgcagcctg aataccggga aggaccgctt
     4681 ctactttgcc gaggtagatg tccccgacgg aacgccggga atcgggacgg tcgaccagga
     4741 gttcaacaag gcggtcgtcc tcgagaatcg ccacgcgggt ttcccgctgg cttcctgaga
     4801 tgagaatttc tcgcttcaaa gcacgaatcc tttgcgcccc cgttgggcca agagcccgag
     4861 ggggtcccaa ggtg
//
LOCUS       DASUBP010000133         2490 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_725895, whole genome shotgun sequence.
ACCESSION   DASUBP010000133 DASUBP010000000
VERSION     DASUBP010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2490)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2490)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2490
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725895"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            41..946
                     /gene="argF"
                     /locus_tag="VFU75_04965"
     CDS             41..946
                     /gene="argF"
                     /locus_tag="VFU75_04965"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEU5466217.1"
                     /translation="MPKRDFLAITDFSKDEISKLFDLAARMKSGQYRETPLAGQTLAM
                     IFAKSSTRTRVSFEVGAYQLGGQALFLSSRDIQIGRGEPIPDTARVLSRYVNGIMIRT
                     FDHGEVEELAKFATIPVINGLTDLSHPCQVLADLFTVKEALGSWEGKRIAWIGDGNNM
                     ANSWLEAAQVLGFELRLACPEGFEPNHAKFEAAKAKTKVLVTESPEEAVAGAHVVNTD
                     VWASMGQEGEAESRKNAFRGYTVDKSLMKQADAKAIFLHCLPAHRGEEVTADVIEGPQ
                     SKVWDEAENRLHVQKALLATLMGSR"
     gene            963..2090
                     /gene="gcvT"
                     /locus_tag="VFU75_04970"
     CDS             963..2090
                     /gene="gcvT"
                     /locus_tag="VFU75_04970"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017950434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HEU5466218.1"
                     /translation="MTDTPPPAPASLKRTPLHDVHVALGAKMVPFGGYEMPVTYPAGI
                     GAEHKAVRTDVGIFDVSHMGEFEITGPDRNAFVQKVTCNDVGALEAGQAQYSAILTDK
                     GTFVDDCLVYRFDDKLMMVVNASNIEKDWNHILDQKGGANVRLRNISDDVGLLAIQGP
                     RAEALLTPLATPKVKDIPYYHFLPDAKVAGVECFISRTGYTGEDGFELYCRAQDTEKL
                     WHSLAGQGRAAPAGLGARDTLRLEAGLPLYGNDIDDTTTPLEAGLNFIVKLEKGAAFT
                     GQAALKKQKLEGVTRRLVGFKMGDPKAIGRHGYPVFLNGRQVDVVRSGTVTPTVNAPI
                     GTTYLPKESAKAGTKFEVEVRGKRAEAEVVKLPFVPHNTKK"
     gene            2098..>2490
                     /locus_tag="VFU75_04975"
     CDS             2098..>2490
                     /locus_tag="VFU75_04975"
                     /inference="COORDINATES: protein motif:HMM:TIGR00177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HEU5466219.1"
                     /translation="MSGVRVNLALRVGILTVSDLGAQGRRDDTSGDAIATWASQRGFE
                     VAVRSIVPDETHLIVSKLTRWSDSGEVDVVLTTGGTGLADRDVTPEATRAAVQRAVPG
                     IAEAIRARGAKGFPRAWLSRGIAGTRGKT"
BASE COUNT          463 a          802 c          831 g          394 t
ORIGIN      
        1 tcattttggc ctgtattgat tgtcattcac tagggacccg atgcccaagc gcgatttcct
       61 ggcaatcacg gacttctcga aggacgagat ctccaagctc ttcgatctcg ctgcccgaat
      121 gaaatcgggc cagtatcggg agacccctct ggcgggccag accctggcca tgatcttcgc
      181 caagtcctcc acccgcaccc gggtctcctt cgaagtcggc gcctatcagc tcggcgggca
      241 ggccctcttc ctctcgtccc gggacatcca gatcggtcgt ggtgaaccga tccccgacac
      301 cgcgcgcgtt ctctcccgtt atgtgaatgg gatcatgatc cggaccttcg accacggtga
      361 ggtcgaggag ctggcgaagt tcgcgaccat ccccgtgatc aacggcctca ccgacctctc
      421 gcacccgtgc caggtgctgg ccgacctgtt cacggtgaag gaggcgctgg ggagctggga
      481 aggaaagcgg atcgcgtgga tcggggacgg caacaacatg gccaacagct ggctcgaagc
      541 cgcgcaggtg ctcggcttcg agctccggct cgcatgcccc gaaggcttcg agcccaacca
      601 cgccaagttc gaagccgcca aggccaagac caaggtgctg gtcaccgagt ctcccgaaga
      661 agcggtggcc ggcgcgcacg tcgtgaacac cgacgtctgg gcatcgatgg ggcaggaagg
      721 ggaggccgag agccgcaaga acgccttccg cggctacacg gtggacaagt ccctgatgaa
      781 gcaggccgac gccaaggcga tcttcctgca ctgcctcccc gcgcaccgcg gcgaggaagt
      841 caccgccgat gtgatcgaag gaccccagtc gaaagtctgg gacgaggccg agaaccggct
      901 gcatgtacag aaggcgctgc tcgcgacact gatgggttca cgctgaattt ccgaggaata
      961 cgatgactga tacgcccccc cccgcccccg cctccctgaa gcgcacgccg ctccacgacg
     1021 ttcacgtcgc cctgggtgcc aagatggtgc ccttcggcgg ttacgagatg ccggtgacct
     1081 atcccgccgg tatcggcgcc gagcacaagg cggtgcggac ggacgtcggg atcttcgacg
     1141 tgtcccatat gggcgagttc gagatcacgg ggcccgaccg gaacgccttc gtgcagaagg
     1201 tcacctgcaa tgacgtcggc gcgctcgagg cgggccaggc ccagtactcc gcgatcctga
     1261 ccgacaaggg cacgttcgtc gacgactgcc tggtctaccg gttcgacgac aagctgatga
     1321 tggtcgtgaa cgcgtcgaac atcgagaagg actggaacca catcctcgat cagaagggcg
     1381 gggccaacgt ccggctccgg aacatctcgg atgacgtcgg tctgctcgcc atccagggcc
     1441 cgcgtgcgga ggcactcctc accccgctcg ctacgcccaa ggtgaaggac attccgtact
     1501 accacttcct ccccgacgcc aaggtcgcgg gcgtggagtg cttcatctcc cgcaccggat
     1561 acacggggga ggacggcttc gagctgtatt gccgcgcgca ggacaccgag aagctctggc
     1621 actcactggc gggtcagggg cgcgccgcgc ctgccggatt gggtgcgcgg gacaccctgc
     1681 gccttgaggc cggtctgcct ctctacggca acgacatcga tgacacgacc acgccgctcg
     1741 aggcgggact caatttcatc gtgaagctgg agaagggcgc ggccttcacc ggtcaggcgg
     1801 ccctcaagaa gcagaagctc gaaggggtga cccgccggct ggtcgggttc aagatgggcg
     1861 accccaaagc catcggccgg cacggatacc cggtgttcct gaacggccga caggtggatg
     1921 tcgtccgctc gggcaccgtg acgccgacgg tgaacgcgcc gatcggcacc acgtatctcc
     1981 cgaaagagtc ggccaaggcc gggacgaagt tcgaagtcga ggtccggggg aaacgagcgg
     2041 aagccgaagt tgtgaagctc cccttcgtgc cgcacaacac caagaaatag gggtcaggtg
     2101 tcaggggtca gggtgaatct cgcattgcga gtcggcattc tgacggtgtc ggatctcggt
     2161 gcccaggggc gccgcgacga cacgtcggga gatgcgatcg cgacgtgggc gtcgcagcgc
     2221 gggttcgagg tggcggtccg gagtatcgtg cccgacgaga cgcacctgat cgtgtccaag
     2281 ctcacgcgct ggtcggactc cggtgaggtg gatgtggtgc tgaccactgg tgggacgggg
     2341 ctcgccgacc gggatgtgac gccggaggcg acgcgggccg cggtccagcg ggccgtgccc
     2401 gggatcgccg aggcgatcag ggcccgcggt gcgaaggggt tcccccgcgc ctggctctcc
     2461 cgcggaatcg caggcacgcg cggcaagacg
//
LOCUS       DASUBP010000134         2758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_357761, whole genome shotgun sequence.
ACCESSION   DASUBP010000134 DASUBP010000000
VERSION     DASUBP010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_357761"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..628
                     /locus_tag="VFU75_04980"
     CDS             <1..628
                     /locus_tag="VFU75_04980"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5466220.1"
                     /translation="TWNYEIGARGASNDLVRWSAAVFQADVHDELIPFQEPSSPQRLF
                     YRNAGSARHRGIELGAAASPVRGLSVALTYTYSHFRYTDYRYAVGATSYVLDGHPLAG
                     VPEHWLRALVGVRPWGPKGGWMELETVHASSQLVNDTLSTRAAGWWRTDLRLGWGGLV
                     GSVRLDPFFAVQNLFDHHYVGSVTVNAAGGRYYEPAPGRNFYVGMEIH"
     gene            complement(736..975)
                     /gene="infA"
                     /locus_tag="VFU75_04985"
     CDS             complement(736..975)
                     /gene="infA"
                     /locus_tag="VFU75_04985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU5466221.1"
                     /translation="MAGSEATDVAGDAIQMMGTVTEVLPNTTFRVKLENGHEVLAYVS
                     GKMRKNYIRILQGDRVAVDLSPYDLTRGRITYRYK"
     gene            1354..1608
                     /locus_tag="VFU75_04990"
     CDS             1354..1608
                     /locus_tag="VFU75_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466222.1"
                     /translation="MDQEYTRLADIEVEAVRPDVQGITLTGQAPDHSEYRLDVHFDMP
                     IDARTRAVLGELLSHSGLTISRRGVAPAAPARRRDRAHRT"
     gene            1686..>2758
                     /locus_tag="VFU75_04995"
     CDS             1686..>2758
                     /locus_tag="VFU75_04995"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5466223.1"
                     /translation="MPPLTSRRRRRVLGLAALALAGGCAGAPVAPPPAPADIPALEAR
                     RAQSPDDPAANLRLGEAYYSAKRYDDARAALGRTLLHQPDNAEAQIYLGYTYEGLGQF
                     DSARVTYNKLSASNPPAAVKKQLNGRLALVAQHQLVYEARRALAQEATLSKTPPPPNT
                     VAVMPFRYTGTDSTYAPLSRGLASLVVTDLGRVHSLRLLERERMQALLDEMKLTESGR
                     VDPATGARSGHLIGASQVVQGQFDVQPNQVRVNATVVRTNDAGVAATGTGADRLQALF
                     DVEKQVVFQLLGKMGITLTPAEQTAISERPTRDLEAFLLYSRGLQAQDQGNFGAASQA
                     FSAAAARDPGFSAAGAAAQSSEQT"
BASE COUNT          438 a          926 c          903 g          491 t
ORIGIN      
        1 cacctggaac tacgaaatcg gtgctcgtgg ggcgtcgaac gatcttgtgc ggtggagcgc
       61 cgcggtgttt caggcggacg tacatgacga gctgattccg tttcaggagc cgtcatcccc
      121 gcagcggctg ttctaccgga acgccggcag cgcgcgtcat cgcgggatcg agctcggggc
      181 cgccgcgtcg cccgtgcgcg ggctctccgt cgccctcacc tacacgtatt cgcactttcg
      241 ctacacggac tatcggtacg ccgtgggggc gacgagctac gtccttgacg gccatccgct
      301 ggcgggcgtg ccggagcatt ggcttcgcgc gctcgtgggg gtgcgtccct ggggcccgaa
      361 gggcggctgg atggagctcg agaccgtgca tgcgtcgagt cagttggtga acgacacgct
      421 gagcacccgc gccgcgggct ggtggcggac tgacctgcgt ctgggctggg gaggcctggt
      481 ggggagcgtg cggctcgatc cgtttttcgc ggtgcaaaat ctcttcgatc atcactacgt
      541 cggatcggtc accgtgaacg cggcgggcgg gcggtattat gagccggcgc cgggaaggaa
      601 tttctatgtg gggatggaaa tccactaaga agacgtcgag gagcgacgtc aaggagaacc
      661 gtcaaggaaa atcgtcaggg ggtagttacg cattcccttg acgtcgttcc tcgacgcatt
      721 tcctcgacgt ttttgttact tgtaccggta ggtaatccgt cccctggtca ggtcgtatgg
      781 agataggtct acagcaactc gatctccctg gaggatgcgg atgtagttct tccgcatttt
      841 tccggagacg tacgccagga cttcgtggcc gttctcgagc ttcacgcgga acgtggtgtt
      901 gggcaacacc tccgttaccg tccccatcat ctggatcgcg tcacccgcca cgtcggtcgc
      961 ctcgctcccc gccatcgcct tcggccgggg aattctgggg atccatggtc gcgtcccgcg
     1021 gcggcagggt gccgccttcg atgtaggacg cgtgtcttcc gcatttgccg caggtcagac
     1081 tgatccgctc gccggccctg ggggtctccc ccgggcgcgc tggtcgcctg cgcggcaccc
     1141 gctgaatcgc ccgagcccca caggagggac aaaccagcgg ttggtgctcg cgatcggccg
     1201 ctatcaggcc cagggcttca gacgcccggt attcctttac tccacgtcgc cccaccgatc
     1261 gactaagacc tccaaggcga aatctaggcg ggccttggtc tccttgacag cccttcgggg
     1321 aggggtatct ctctgagagg ccggaggata gctatggacc aagaatacac gcgtctggcg
     1381 gacatcgagg tcgaggcggt gcgcccggac gtccagggaa tcaccctgac cggccaggct
     1441 cccgaccatt ccgagtaccg cctggacgtg catttcgaca tgccgatcga cgcgcggact
     1501 cgcgcggtgc tcggtgaatt gctgtcgcat tcaggactga ccatatcccg ccggggagtg
     1561 gcaccggcgg ctcccgcgcg gcgacgtgac cgcgctcacc gtacctgacg gcggggggtc
     1621 accgaccctc ccactggccg aatttacctt cagctaaacg agtcgacatg tcaccctcac
     1681 ccgcgatgcc ccccctgacc tcgcgccggc gccggcgtgt cctcggcttg gcggctctcg
     1741 cgctcgccgg tggctgtgcc ggtgcgcccg ttgcgccgcc tcccgctccc gccgacatcc
     1801 cggccctgga agcccgccgg gcacagtcgc cggacgatcc cgccgcgaat ctgcgcttgg
     1861 gtgaggccta ttactccgcg aagcggtatg acgatgcccg ggccgctctg ggccgcacgc
     1921 tgctgcatca gcctgacaat gccgaagcgc agatctacct cggctacacc tatgaaggcc
     1981 tgggccaatt cgactcggcc cgcgtcacct acaacaagct gtcggcatcc aatccgccgg
     2041 ccgcggtgaa gaagcagctg aacggccgcc tggcactcgt cgcgcagcac cagctggtct
     2101 acgaagcgcg tcgggcactg gcgcaggaag cgacgctttc gaagactcca ccgccgccca
     2161 atacggtggc cgtgatgccg ttccgctaca ccgggaccga ttccacgtat gctccgctct
     2221 cccgcgggct ggcctcgctc gtggtcaccg acctcggtcg ggtgcacagc ctgcggctcc
     2281 tcgagcgtga gcggatgcag gcgttgctcg atgaaatgaa attgacggaa tcgggccgcg
     2341 tggatccggc gacgggcgcg cgctccggcc atctcatcgg tgcttcgcag gtggtgcaag
     2401 gccaattcga cgtgcagccc aatcaggtgc gcgtgaacgc gaccgtcgtc cgcacgaacg
     2461 acgcgggcgt ggccgcgacc ggcaccggtg ccgaccgcct ccaggcgctc ttcgacgtcg
     2521 agaagcaggt cgtcttccag ctgctcggaa agatgggcat cacgctgacc ccggccgagc
     2581 agaccgcgat cagcgagcgt ccgacccgcg acctcgaagc gttcctgctc tacagccgcg
     2641 gactccaggc gcaggaccag ggcaacttcg gcgcggcttc gcaggccttc tcggcggcgg
     2701 cggcccgtga tcccggcttc agcgcggcag gcgcggcggc gcagagcagc gagcagac
//
LOCUS       DASUBP010000135         9195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_98936, whole genome shotgun sequence.
ACCESSION   DASUBP010000135 DASUBP010000000
VERSION     DASUBP010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9195
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98936"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..945
                     /locus_tag="VFU75_05000"
     CDS             <1..945
                     /locus_tag="VFU75_05000"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU5466224.1"
                     /translation="RWTGVSWRDFVFDGLDWAIYGLLTPAVFWLGRRYPLQRGTLRRA
                     LPIHVMGALALCVAWAVLGTLLRHLLHAGSENLLGWILTTLPFGVAVYFAMLGVQHAT
                     TYFTQSRALSVQLADARLAALRMQLHPHFLYNSLNAATVIVRDRDSATAARMLELLGE
                     MLHRVIRADRPQEVLLRDELEFVRQYLTIEQIRFPDRLHPEITVPDGLLDALVPDFIL
                     QPLVENAIRHGLAQRVGATMLRVEAHRDGEDLVLTVTDDGPGPGAATSTEGVGLANTR
                     ERLTTLYGGRGSLTLTRTPSGGAMATVRLPFRSGTDRG"
     gene            938..1735
                     /locus_tag="VFU75_05005"
     CDS             938..1735
                     /locus_tag="VFU75_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEU5466225.1"
                     /translation="MADIRALIVDDEPLARRGIRQLLAAHPDVVVIGECRDGREAVRA
                     LATTKPDLVFLDVQMPGLDGLGVVRVHGAERMPVTVFITAHDQFAVDAFEAQALDYLV
                     KPLSEDRFREALQRVRERLRMHDALALAAKLSALLRGEPVTTNGTGRAPRTPIAVPTD
                     NGELLLDQGEIDWIEAADDYAVIHAGGKSHRVRAALSALESRLDPARFTRIHRSSVVR
                     LDRVREWKTDTDDEMVLILRDGTELPVSRRRAAQVKALLRPPAPRTH"
     gene            1809..3017
                     /locus_tag="VFU75_05010"
     CDS             1809..3017
                     /locus_tag="VFU75_05010"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase
                     domain-containing protein"
                     /protein_id="HEU5466226.1"
                     /translation="MTSSPANTAPSRLSSIDVIRGAIMVLMAIDHVRVYSGLPAGGPT
                     AGIFFTRWVTHFCAPGFIFLAGTSAFLSSRRHPDISRHLLIRGAWLVFLELTFLRVAW
                     TFNFDFANYEMAGVIWCIGWCMIILGLLVRLPLRTVVGFGAFVVLGASLLDPVFGRVI
                     QAAGASPLSGLWKILYVGFFAGPIQFGSHGPTLIVLYSIVPWVGVMALGYGFGALITL
                     EPVRRNRLCWQIGLSATGLFLVFRGFNLWGDPSHWHFPQANANGRPQLPALFSFLSTN
                     KYPASLDFQLMTLGPTITAIPLLEGVRRMATGWLTVFGRVPFFYYMLHIPLIHALAVV
                     VSALREGHVNPWLFTNHPMGNPAPPDGYTWPLWLLYVVWAVAIFILYFASRWFGALKA
                     RRTDWWLRYL"
     gene            2999..4339
                     /locus_tag="VFU75_05015"
     CDS             2999..4339
                     /locus_tag="VFU75_05015"
                     /inference="COORDINATES: protein motif:HMM:NF024206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HEU5466227.1"
                     /translation="MAQVPVEEDDPRAAFIHAAIWHGNLDQAEQIRGRHPSLIGADLV
                     TAAVLGDDVRVRNFIAADPASVNRKGPPFGGDALNYLALSKYLRLEPARTPGFLRAAT
                     ALLDAGADPNTGFWTDPPHPEFETALYGAAGVAHHEGLTRLLLERGADPNDGEVAYHS
                     PETDHLGALGALIETGRLTQNSLSIMLIRSCDWHYLDAVKLLLDSGANPSHRAKWGLS
                     PLLHAIARDNGLEIIKALLDHGADANRRDPHSRTKEPMTAIQLAARKGRGDILEELDR
                     RRIPVRLEGLDRLLELAARGDADGARELAKDEGVSRRLTADGARALGDFTRTWNTDGV
                     RILLDLGVPVDVRSEGDSYFGTPPDSTALHVASWEVLPKLVQLLLDRGADPNARDSQG
                     MTPLMLTVRGCTASWWAHRRTAEPAQLLLAAGASRDGVTVPTGYDELDRVLEAH"
     gene            complement(4351..7185)
                     /locus_tag="VFU75_05020"
     CDS             complement(4351..7185)
                     /locus_tag="VFU75_05020"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU5466228.1"
                     /translation="MIALEPGTRIDVYRLIRPLGKGGFAEVWEAKNEADGRHVALKVL
                     TELRADSERALERFEQEGRLAASLSSPRCVYVFGAGAVEACPYIAMELMTGGTLAGRI
                     ESGSLDARSAVDCVLDVLEGLEAAQRQGILHRDVKPTNVFLDAEGRAKIGDFGISKSL
                     EGDAKLTQTGAFLGTPYYASPEQIAAEPLDLRSDLYSVGAMLYELLTGKLPYAGTNAS
                     QVLAQVLTRDPVPFAQTGVAVPLGLQQVVMRLLAKQREKRFQSYPDTRDALLPFSSRG
                     LVVAPLPRRFGAILIDLALFFPLSFMVGAFLLQRDFRAQLPVSLSVHVLELAYFTLLE
                     RYGGASLGKRILGLRVTTVDRGSPAMPRVLVRSLIFLLLLVGPSIGVQQLAAAGVPIG
                     SGPLLTWMPALFELLGVALLVSPMRKANGYAGTHELITHTRVMALAGRERASTVAPVP
                     AAAMSGAAGQEFGPYRGTGVLWSRDGESLMLARDAALGRDVWVHVAGEGGLPPLEVVR
                     ERGEGSLPWLQRGSSEGAVWDAYGAPAGTALGSEGRLDWGQMRGVLHSVAAELSDRFK
                     RQGTAGHLSPDYVWVDPGSRALLLDFPVTPGGAPAVEVTPANWWTFLGMVATRGLRWP
                     SVPLPEHGRALLAKLAIPTGPGPIDEFVAALDRVADRPTRVTRLRRLGPLAMFAILPV
                     LMVVVSLVVPVFMEGLPTWYQDLSLNAESLIDSLRSATARAGTDTVAARTARAIQVVL
                     ARDRHEAGLTPQLGKPALNALPLRARAEVDSAALRYPSPDARMVADARAWLATRVPAR
                     HLHSGPATSFRMFVTAFGITGYLGILGVLLAAALRGGLLFTLFGIALQQADGTPASRL
                     RCFARSLVAWAPLLLLAALSEVSNLSVTPPRPGVTVSATSVPSEPSAPRSDVIDDLLV
                     LVAIGGAGFAVWRPTQGIAERVAGVVLVPK"
     gene            7274..>9195
                     /locus_tag="VFU75_05025"
     CDS             7274..>9195
                     /locus_tag="VFU75_05025"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HEU5466229.1"
                     /translation="MKSVCLLVVALLPLSPARAQRRADITRWERESRNVTIIRDDWGI
                     AHVYGKTDADAVFGMEYAQAEDDFNRVETNYLNALGWLARAEGASAIFQDLRQQLFID
                     TDSLKSEYASSPVWLKKLMDAFADGLNFYLYKHPDVKPRAITHFEPWMALSFTEGSIG
                     GDIERVNIGQLAAFYGDSLQRPARLTGGDGIQDEPTGSNGIAVAPSRTADHHAILWIN
                     PHTSFYFRSELQMVSDEGLDAYGAATWGQFFIYQGFNTKVGWMHTSSGVDNIDEFLET
                     VTTAGKRHTYLHGGRQFPVTTRSITISYATPTGMSQRTFTAYFTRHGPVVRKQGVQWV
                     TISLMQSPVNALIQSYARTKAGNLAEFEKFFDLHTNSSNNTVFADAQGDIAYFHSNYI
                     PRRDTTFDWTKPVDGSNPATDYHGLLSVDETPKVVNPPSGWLYNSNNWPWSAAGADSP
                     RRSEFPRYVETGTEESFRGMHALRVLPALHDATPDSVIGAAFDSYLPGFAQMLPALLK
                     AYDDAPAGDSLKNKVAEQIGLLRGWDYRWGANSVPTSLAVFWGEAAIRRVFRDARAAN
                     LSPWDYVSQRATGPELLGALAAASDTLTADFGSWRTPWGEINRFQRIDDQIASHFDDS
                     KPSIPVPFTSSLWGSLA"
BASE COUNT         1564 a         3189 c         2922 g         1520 t
ORIGIN      
        1 cgttggaccg gcgtgtcgtg gcgggacttc gtattcgacg gattggattg ggcgatttac
       61 gggctcctca cccccgccgt cttctggctg ggccgccgct accctctcca gcgcggcacc
      121 cttcgtcgcg cgctccccat ccacgtcatg ggcgcgctcg ctttgtgcgt cgcctgggcg
      181 gtcctgggca ccctcctgcg acatctcctc cacgcgggca gcgagaacct gctcggctgg
      241 atcctgacca cactgccctt cggggtcgcc gtgtacttcg ccatgctggg ggtccaacat
      301 gcgacgacgt atttcaccca gtctcgggcg ctcagcgtcc agctcgccga tgcccgcctg
      361 gcggccctcc gcatgcagct gcacccccac ttcctctata acagtctgaa cgcggccacc
      421 gtcatcgtgc gggaccgcga ctccgccacc gccgcgcgga tgctcgagtt gctcggggag
      481 atgctgcatc gcgtgatccg ggccgaccgt ccccaggaag tgctcctgcg ggacgagctc
      541 gagttcgttc ggcagtacct cacgatcgag cagatccggt tccccgatcg cttgcacccc
      601 gaaatcactg tgcccgatgg cttgctggat gccctcgttc ccgacttcat cctccaaccc
      661 ctcgtggaaa acgccattcg gcatgggctc gcgcagcggg tgggagcgac catgttacgg
      721 gtcgaagccc accgggatgg agaggatctg gtgctgaccg tgaccgatga cgggccgggc
      781 ccgggagcgg ctacttccac ggaaggggtc gggttggcca atacccgcga gcgcctcacg
      841 accctgtacg gaggccgggg ttcgctcacc ttgacccgca cgccgtccgg tggagcgatg
      901 gccacggttc gcctgccctt ccggtccggg acggatcgtg gctgacattc gcgcgctgat
      961 cgtcgacgac gagcccctgg cgcgccgggg aatccgccag ctcctggcgg cgcacccgga
     1021 cgtcgtggtg atcggcgaat gccgggacgg ccgggaggcg gttcgagcgc tggcgaccac
     1081 caaaccggat ctcgtgttcc tcgacgtcca gatgccggga ctcgacggcc tgggcgtggt
     1141 ccgcgtgcat ggcgcagagc ggatgccggt gacggtgttc atcacggcgc acgatcaatt
     1201 cgccgtggac gccttcgaag cacaagcgct cgactacctg gtcaagcccc tttccgaaga
     1261 ccggtttcgt gaggcgctcc agcgcgtccg ggagcgcctg cggatgcacg atgccttggc
     1321 gctcgccgcc aagctctcgg cgctgctccg cggggaaccg gtgacgacga atggcacggg
     1381 tcgcgcgccc cgcacgccca tcgccgtccc gacggacaat ggtgagctgc tgctcgatca
     1441 gggagaaatt gattggatcg aagcggcgga cgactacgcg gtgatccacg cgggcgggaa
     1501 gtcccatcgc gtgcgcgccg cgctctccgc actcgagtcc cggctcgacc ccgcccggtt
     1561 cacccggatc caccggtcga gtgtcgtccg cctggaccgg gtccgcgaat ggaaaaccga
     1621 caccgatgac gagatggtcc tgatactgcg ggatggcacc gagctgccgg tgagtcgccg
     1681 ccgggccgcg caggtcaagg cgctcctgcg gccgcccgct ccccgcaccc actaggccgc
     1741 tcgccgcctc ccgggaaccg ccggcccgcc gacgttgacg ggcgctcacg cgacccaaag
     1801 cttgcggaat gacctcatcc cccgcgaaca ccgcgccgtc gcgcctctcc tccatcgacg
     1861 tgattcgggg cgccatcatg gtgctgatgg cgatcgatca cgttcgggtg tactccggcc
     1921 ttccggccgg agggcccacg gccgggatct tcttcacccg gtgggtgact cacttctgcg
     1981 cgccgggctt cattttcctg gccggcacca gcgcgtttct ctcctcccgc cggcacccgg
     2041 acatttcacg gcacttgctc atccgcggcg cctggctcgt cttcctggag ctgacgttcc
     2101 tgcgcgtcgc ctggacgttc aatttcgact tcgccaacta cgagatggcc ggcgtgatct
     2161 ggtgcatcgg ctggtgcatg atcatcctcg gccttctcgt ccggctcccc ctgcgcacgg
     2221 tggtcggatt cggcgccttc gtcgtcctcg gcgccagcct gctcgatccg gtattcggac
     2281 gggtgatcca ggcggcgggt gccagtccgt tgagcgggct ctggaagatt ctctatgtcg
     2341 gcttcttcgc cgggcccatt cagttcggct cgcatggccc aacgctgatc gtcttgtact
     2401 cgattgtgcc ctgggtggga gtgatggcgt tgggatacgg cttcggcgcc ttgatcacgc
     2461 tggaacccgt acgccggaac cgcctctgct ggcagatcgg tctctcggcc acggggctct
     2521 ttctggtctt ccgcggcttc aatctctggg gtgatccgag ccactggcat ttcccgcagg
     2581 caaacgcgaa tggccggccg cagctgccgg cgctcttctc gttcctgagt accaacaagt
     2641 acccggcctc cctcgatttc cagctgatga ccctggggcc caccatcacc gcgattccgc
     2701 tgttggaggg ggttcggcgg atggcgaccg gctggctgac cgtcttcggc cgcgtcccat
     2761 tcttctacta catgcttcac atccccctga ttcacgcgct cgccgtggtg gtctccgccc
     2821 tccgggaggg gcacgtgaat ccctggctct tcaccaatca ccccatgggc aatcccgcgc
     2881 ctccggacgg atacacctgg ccgctgtggc tgctgtacgt cgtctgggcg gtcgcgatct
     2941 tcattctgta ctttgcctca cgctggttcg gggctctcaa ggcgcggaga acggattggt
     3001 ggctcaggta cctgtagagg aagacgatcc gcgggcggcg ttcattcacg ccgccatctg
     3061 gcatgggaac ctcgaccagg ccgaacagat tcgcgggcgg catccgtcgc tgatcggcgc
     3121 cgacctggtg acggcggccg tgctggggga cgacgtccga gtccggaact tcatcgccgc
     3181 ggatccggcg agcgtcaacc gcaagggacc gccctttggg ggcgatgccc tcaactacct
     3241 cgcgctttcc aagtacctgc ggctcgagcc ggcacggacg ccgggattct tgcgggccgc
     3301 gaccgccctg ctcgacgccg gtgccgaccc caacaccggg ttctggaccg atcctccgca
     3361 tccggaattc gagaccgcac tctatggcgc cgccggcgtc gcgcaccatg agggtctcac
     3421 gcggctcctg cttgaacgcg gtgcggatcc caacgacggc gaagtcgcct accactctcc
     3481 ggagaccgac catcttggtg cccttggggc actcatcgaa acggggcggc tgacacagaa
     3541 cagtctctcc atcatgctga tccgcagctg cgattggcac tatctcgatg ccgtgaaact
     3601 gctcctcgac agtggcgcca atccttctca ccgggcgaag tggggactaa gccccttgct
     3661 gcatgccatc gcccgcgaca acgggttgga gatcatcaaa gcgcttctgg accatggcgc
     3721 ggacgccaac cgccgggatc cgcacagccg aaccaaggag cccatgaccg cgatccagct
     3781 cgccgcccgc aagggacggg gagacatcct cgaagagctc gaccgccgcc gcatccccgt
     3841 ccgactcgag ggactggatc gcctgctgga gctcgcggcg cggggggatg ccgacggggc
     3901 gagggagctc gcgaaagacg aaggcgtcag ccgtcggctg acagcggacg gcgcacgtgc
     3961 gctcggtgac tttacccgga cctggaacac ggacggcgtc cgcatcctgc tggatcttgg
     4021 cgtaccggtg gacgtccgct cggaggggga ttcctacttc ggaacgccac ccgacagcac
     4081 ggcgctgcac gtagcctcgt gggaggtgct cccgaagctg gtacagctgt tgctcgatcg
     4141 tggtgccgat cccaacgcgc gggattcgca gggcatgacc ccgctgatgc ttaccgtccg
     4201 gggttgcacc gcgtcgtggt gggcgcatcg cagaaccgcg gaaccggcac agctcctgct
     4261 cgcggccggt gcaagccgcg acggcgtgac ggtgccgacg gggtacgacg agctggaccg
     4321 ggtgcttgag gcccactgag cctccaggca ttacttcgga acgagcacca ctcccgccac
     4381 ccgctcagca atgccctgcg tcggacgcca gacggcgaat cccgcacccc cgatcgcaac
     4441 caggaccagg aggtcatcga tgacatccga tctgggagcg gatggctccg atggtacgga
     4501 ggtcgccgag accgtcacac cgggccgtgg aggggttacc gagaggttcg atacttcgct
     4561 caacgccgcg agcagaagca gcggcgccca agcgaccagt gaccgggcga agcagcgcag
     4621 acggctcgcg ggagtcccat cggcttgctg aagagcgatg ccgaacagcg tgaacagcag
     4681 accgccccgc agggcggcgg ccagcaaaac gcccaggata ccgagatacc cggtgatgcc
     4741 gaaggccgtg acgaacattc gaaaggaggt ggcggggccg gagtgaaggt gacgggccgg
     4801 cacgcgcgtg gcgagccagg cgcgtgcgtc cgcgaccatt ctggcatccg gtgacgggta
     4861 ccggagtgcc gcggaatcga cttccgcgcg ggcccgcagc ggcagggcat tcagcgcggg
     4921 tttgccgagc tgcggtgtca ggcccgcctc gtgccggtct cgcgccagga cgacctgtat
     4981 cgcccgggcg gtgcgcgccg ctacggtgtc ggtcccggcc cgggcggtgg cgctgcgcag
     5041 tgaatcgatg agtgactcgg cattcagcga gaggtcctga taccaggtgg gaagaccttc
     5101 catgaagacc ggcaccacga ggctcaccac caccatcagt accggcaaga tggcgaacat
     5161 ggcgagaggg ccgagccgcc gcaggcgggt gacgcgcgtg ggacggtctg cgacgcggtc
     5221 caacgccgcc acgaattcat caatggggcc aggtcccgtc gggatggcga gcttggcgag
     5281 caaagcccga ccatgctcag gcagtggcac cgagggccac cgaaggccgc gcgtcgctac
     5341 catccccagg aaggtccacc aattcgcggg cgtcacttcc accgccgggg ccccaccagg
     5401 cgtcaccgga aagtcgagga ggagagcgcg actcccggga tccacccaga cgtaatccgg
     5461 gctcaggtgg cccgctgttc cctgacgctt gaaacggtcg ctgagctctg ccgccaccga
     5521 atggagaaca ccgcgcatct gaccccagtc gagccgcccc tccgacccga gggccgttcc
     5581 cgccggcgcg ccgtaggcgt cccagaccgc gccttctgat gatccgcgct gcagccatgg
     5641 gaggcttccc tcaccacgct cccgcacgac ttcgagcggc gggagcccgc cttcccctgc
     5701 cacgtgcacc cagacgtcgc gcccgagggc ggcgtcccgc gccagcatga gtgactcccc
     5761 atcccggctc cagagaacgc cggtcccgcg atagggtccg aattcctgac ccgcggcccc
     5821 ggacatcgcg gctgccggca ccggcgccac cgtactggcc cgctcccggc cggccaacgc
     5881 catgacgcgc gtgtgtgtga tcagctcgtg cgtccccgcg tacccgttgg ctttccgcat
     5941 cggcgagacg agcagggcga cgcccagaag ctcgaacaac gccggcatcc acgtcagcag
     6001 agggccactc ccaatcggca cccccgcggc cgcgagctgc tgcacgccga tactcgggcc
     6061 gacgagcagc agcaggaaaa tcagggagcg caccaggaca cgcggcatcg cgggactccc
     6121 ccggtcaacc gtggtgaccc gcagaccgag gatgcgcttt ccgagactcg ccccgccgta
     6181 ccgctccaac aacgtgaagt acgcgagctc cagaacatgg accgagagcg agaccggcag
     6241 ctgcgcgcgg aagtcgcgtt gcaacagaaa ggcgcccacc atgaagctta gggggaagaa
     6301 cagtgccaga tcgatgagga tggcgccgaa gcgccgaggc agaggtgcaa cgacaagtcc
     6361 acgggatgag aatggcagca gcgcatcccg cgtatcgggg tagctctgaa agcgcttttc
     6421 ccgctgtttg gcgagaaggc gcatcacgac ctgctgcagt cccagcggga cggcgacgcc
     6481 ggtctgcgca aatgggaccg gatcgcgagt gaggacctgc gccagaacct gggaggcgtt
     6541 ggtaccggcg tatgggagct tccccgtgag cagctcatac agcatggcgc ccacgctgta
     6601 gagatcgctg cgaagatcca gcggctccgc cgcgatctgc tcgggtgacg cgtaatacgg
     6661 cgtccccaga aacgcaccgg tctgggtgag cttggcgtca ccttcaaggc tcttcgaaat
     6721 cccgaagtcg ccgatcttgg cccggccttc ggcgtccaga aagacgttgg tcggcttcac
     6781 atcccgatga agaatgccct gacgctgtgc cgcctccagt ccctcgagga cgtccagcac
     6841 acagtcgacg gccgagcgcg cgtccagcga acccgattcg atacgaccgg cgagcgtgcc
     6901 tcccgtcatc agctccatcg cgatgtaggg gcacgcctcc acggccccgg caccgaagac
     6961 gtagacgcag cgaggactcg acagcgacgc cgcgaggcgt ccttcctgtt cgaatcgctc
     7021 caaggcgcgt tccgaatcgg ctcggagctc ggtgagcacc ttcagcgcga catggcgccc
     7081 atcagcctca ttcttggctt cccacacctc ggcgaaaccg cctttgccca gcgggcggat
     7141 caagcggtag acgtcgatgc gcgtgccggg ttcgagggcg atcactggcg aagaaagtac
     7201 gtcgttgaca cgttcgcgcg agaggcctac cgtcagggac acctgacact gtcctcctct
     7261 ccaagagatt gccatgaagt ccgtgtgcct gttggtcgtc gccctcctcc ccctctcccc
     7321 ggcacgtgcc cagagacggg cggacatcac ccgctgggag cgggagtcca ggaacgtcac
     7381 gatcattcgc gacgactggg gcatcgccca cgtgtacggc aagaccgacg cggacgcggt
     7441 gttcggaatg gaatacgcac aggccgaaga cgatttcaac cgggtcgaga ccaactacct
     7501 caatgcgctg ggctggctgg cccgcgccga aggcgcctcg gccatcttcc aggatctgcg
     7561 gcagcagctg ttcatcgata ccgattccct caaatccgaa tacgcatcca gtccggtgtg
     7621 gctgaagaaa ctcatggacg ccttcgccga cggactgaat ttctatcttt acaagcaccc
     7681 cgacgtgaag ccgcgcgcca tcacgcattt cgaaccctgg atggcactga gcttcaccga
     7741 aggctcgatc ggcggagaca tcgagcgggt caacatcggc cagctggcgg cattctacgg
     7801 agactccctg cagcggcccg cgcgcctcac gggcggtgac gggatccagg acgagccgac
     7861 cggctcgaac gggatcgccg tcgcaccatc ccgcaccgcc gatcaccacg cgattctctg
     7921 gatcaaccct cacacgtcgt tctacttccg gtccgagctc cagatggtga gcgacgaggg
     7981 cctcgatgcc tatggggcgg cgacgtgggg gcagttcttc atctatcagg gcttcaacac
     8041 caaggtcggc tggatgcaca cgtcgtccgg ggtcgacaac atcgacgaat tcctcgagac
     8101 cgtgacgacc gccggaaaga ggcacaccta ccttcacggt gggcggcaat tccccgtcac
     8161 gacgcgctcc atcacgatat cgtacgcgac ccccaccggc atgtctcagc ggaccttcac
     8221 ggcctacttc acccggcatg gccccgtggt tcggaagcaa ggggtccaat gggtcaccat
     8281 cagcctgatg caaagtccgg tgaatgccct gatccagtcg tacgcgcgca ccaaggccgg
     8341 gaacctggcg gagttcgaga aattcttcga cctccacacc aactcctcca ataacacggt
     8401 cttcgccgac gcacagggcg acatcgcgta cttccactcg aactacatcc ccaggcggga
     8461 tacgactttc gattggacca aaccggtcga cgggagcaac cccgctaccg actatcatgg
     8521 gctcctgtcg gtcgatgaaa cgccgaaggt cgtcaatccg ccctccggct ggctgtacaa
     8581 ctcgaacaac tggccctggt cggcggcggg agccgattct cccaggcgga gcgagttccc
     8641 gcggtacgtg gaaaccggca ccgaggagag tttccggggc atgcacgcgc tccgcgtgct
     8701 ccctgccctc cacgatgcca cgcccgactc ggtgatcggc gcggcctttg acagctatct
     8761 gcccggtttt gcgcagatgc tgcccgcgct gctcaaggcc tacgacgacg ccccggccgg
     8821 cgactcgctg aagaacaagg tcgcggaaca gatcggcctc ctgcgcggct gggactaccg
     8881 ttggggcgcg aactctgtcc ccacgtccct cgccgtattc tggggcgaag ccgcgatacg
     8941 acgggtcttc cgcgatgccc gcgccgccaa cctgtctccg tgggactacg tctcccagcg
     9001 cgccaccgga ccggagctcc tcggcgccct agcggcggcg agcgacacac tgaccgccga
     9061 ttttggatcg tggcgtaccc cgtggggcga gatcaaccgc ttccagcgga tcgacgacca
     9121 gatcgcgtcc catttcgatg attcgaagcc gagcattccg gtgcccttca cgtcgtccct
     9181 gtggggatcg ctcgc
//
LOCUS       DASUBP010000136         4503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1297927, whole genome shotgun sequence.
ACCESSION   DASUBP010000136 DASUBP010000000
VERSION     DASUBP010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4503
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1297927"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..791)
                     /gene="murI"
                     /locus_tag="VFU75_05030"
     CDS             complement(<1..791)
                     /gene="murI"
                     /locus_tag="VFU75_05030"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEU5466230.1"
                     /translation="MNDAPLGVFDSGLGGLTVARALFERLPRESVIYFGDTARVPYGP
                     KSADTVRRYSSEILKFLLERGVKMVVVACNTSTAHALDHLRKISPVPVIGVIEPGARA
                     AVTASKGGTLGVIGTVGTISSGAYQRAIAALNPKAVVEGVACPLFVPLVEEGWLDHPA
                     TELIAREYLAPFIKQAPDAVVLGCTHYPLLRAVLGRVLGAGVTLIDSGGETAKAVQEA
                     LESKTLQAGNSRAPSHHFVVSDDAPRFRKIGATFLGDRLRDTEVEV"
     gene            complement(788..1126)
                     /locus_tag="VFU75_05035"
     CDS             complement(788..1126)
                     /locus_tag="VFU75_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466231.1"
                     /translation="MNALWRSMLLPGWGQAVTHRNGTGAAFAAWEGVTIMMTLKAHQE
                     ANYFSRTGSPNVHAKRQEVQDWTVLWIFNHLFAGAEAFVSAHLQDFPKDLHFQAFPRG
                     AGISIPIGRW"
     gene            complement(1237..2145)
                     /locus_tag="VFU75_05040"
     CDS             complement(1237..2145)
                     /locus_tag="VFU75_05040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEU5466232.1"
                     /translation="MGRGIPHLTAHLVVNPAAGRGTARRLKDRVARAFQREGWSVESV
                     DTRGPGHGSTLAAEFSRAGAERVIAVGGDGIVHEVANGLLSAGGRAALGVVPAGSGND
                     FAKLLGVHGHAPEPAVRRIASATVRRFDAGKVAGEYFVNTLGFGFGPEVVRIRDGMPG
                     LKGFLSYLVPVLKAFAGFEPPVVEVRAGESREQGPVMMVEVCNGTTAGGSYRFAPAAD
                     PADGVLDVCVIRRVGLVRFLTALPRVMKGTHGTMHEVKFLRAREITICGEEPLVMHLD
                     GELRMPGTRECTITVEPGALPVLVAS"
     gene            complement(2121..2483)
                     /locus_tag="VFU75_05045"
     CDS             complement(2121..2483)
                     /locus_tag="VFU75_05045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466233.1"
                     /translation="MSRGAIGVKSKWARLRDDVNVRLRRGMWYRVLDEGELECVIEVG
                     GKPKSILRAMIEIVSRPHPKWTVVRAPKTGARASLGDPYGVCPSCGERSKLGKAKRLK
                     CPSCKWEFEVAWDEGYLT"
     gene            complement(2480..4189)
                     /locus_tag="VFU75_05050"
     CDS             complement(2480..4189)
                     /locus_tag="VFU75_05050"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU5466234.1"
                     /translation="MRHHLLLAGLAVAALPLSLSAQRPEREVFTHADTIRGSNTPERA
                     WWDVTFYDLHVAVSPSDSSIKGWNGITYRITGPSKEMQIDLQEPLQVDSMLQDGKKVT
                     YRRDGNAFYVTLTAPQPSGSVKTVSVYYHGRVRVARRAPWDGGFVFAKDSLGRDWIAT
                     ACEGLGASVWWPNKDYLADEPDSQRIAITVPDPLQDVSNGRLRSTTHNKDHTTTYEWF
                     VTEPINNYDVAVNAAVYAHFEQDYAGESGKLTMNFWPLAYHLDTAKVQFQQSVTMMKC
                     FEHWFGPYPWYKDGYQLVETPHLGMEHQSAVAYGNHYKDGYYSAATGTGIDLSRSGQG
                     SNWDFIIVHESAHEWFGNSITAKDQADMWIHEGFANYSENLFVECLRGKEAGADYVIG
                     TRFQVRNDAPIIGPYGVNRDGSGDMYYKGGNLLHTIRQLVSDDDKWRDILRGLSHTFY
                     HQTVTTQDIENYISTRAGMNLSTVFDEYLRTTMIPVFEYKIVDSTLTYRWADVVPRFD
                     MPIRVTLSDGSYTLIHPAAGLQQAAVHLSSPDAFHVDPNFYVYTSRVVDFTGGKPVTG
                     GTR"
     gene            complement(4281..>4503)
                     /locus_tag="VFU75_05055"
     CDS             complement(4281..>4503)
                     /locus_tag="VFU75_05055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466235.1"
                     /translation="GAWQRARWVPKAAVFLAAWVGIPTFASAAGVVRVITQHASPAVG
                     LSFGMVLLGAICQLLALILALQGLEERKA"
BASE COUNT          792 a         1512 c         1440 g          759 t
ORIGIN      
        1 acctcgacct ccgtatcgcg cagccgatcc ccgagaaacg tcgctccgat tttccggaac
       61 cgcggtgcat catcggacac cacgaagtgg tgggatggcg cccgcgagtt ccccgcctga
      121 agggtcttgg actcgagtgc ctcctgcacc gccttcgccg tctccccccc gctgtcgatc
      181 agcgtcacgc ccgctccaag cacccgtccc aggacggctc tgagcaaggg gtagtgagtg
      241 catccgagca cgacggcatc cggggcttgc ttgatgaagg gcgcaagata ctcgcgcgcg
      301 atgagctccg tcgccggatg atcgagccac ccctcctcca ccaacggcac gaagagcgga
      361 caggcgacac cttctaccac ggccttcggg ttgagggccg cgatggcgcg ctggtaggcg
      421 cccgacgaaa tcgtgccgac ggtcccgatg acgccgagcg tgccgccttt ggaggcggtg
      481 acggcggcgc gggcgccggg ctcgatcacg ccgatcaccg gaacggggga aatcttccgg
      541 agatgatcga gcgcgtgtgc cgtgctggtg ttgcacgcga cgacgaccat cttgacgcca
      601 cgttcgagca ggaacttgag gatctcgctc gagtagcgcc ggacggtgtc cgcggacttc
      661 ggtccgtagg ggacgcgcgc ggtatcaccg aaatagatga cggattcccg cggcagccgc
      721 tcgaagaggg cgcgcgccac cgtcagtccg ccaaggccgg agtcgaagac gccgaggggg
      781 gcgtcgttca ccagcggccg atcgggatgg agatgccggc ccctcgcggg aaggcctgga
      841 agtggaggtc cttgggaaaa tcctgcagat gggccgacac gaaggcttcg gcgcccgcga
      901 acaggtggtt gaagatccag agcacggtcc agtcctgcac ttcctggcgc ttggcgtgca
      961 cgttgggcga accggtccgc gaaaagtagt tggcttcctg atgggccttc agggtcatca
     1021 tgatcgtgac cccttcccac gcggcgaatg ccgctcccgt gccgttccgg tgcgtcaccg
     1081 cctgacccca ccccggcaag agcatcgagc gccagagggc gttcacggcg cgcacctcat
     1141 gcaaggtgtc aggggacgtg gggacggcgc gggtcgagtc ttgcgcaaag aggccaagtg
     1201 gccaggtcgc taagaggcta agaaaaagca aagtcctcac gatgccacca acaccggcag
     1261 cgccccgggc tccaccgtga tggtgcactc ccgggtgccg ggcatgcgca gctcgccatc
     1321 caagtgcatc accagcggtt cctctccgca aatcgtgatc tcccgggcgc gcaggaactt
     1381 cacctcatgc atcgttccgt gcgtcccctt cattactctg ggcaaggccg tcagaaaccg
     1441 cacgaggccc acgcgccgaa tgacacagac gtcgagaacg ccatcggccg ggtcggcagc
     1501 aggcgcaaat cggtagctcc cgcccgccgt ggtcccgtta catacctcta ccatcataac
     1561 gggcccttgc tccctcgact cccccgcccg gacctccacc accggcggct cgaaccccgc
     1621 gaaggccttg agcacgggca ccaggtagga caggaacccc ttcaagcccg gcatgccatc
     1681 ccggattcgc accacttcag ggccgaatcc gaatccgagc gtgttcacga aatactcccc
     1741 agccaccttc cccgcgtcga accgccgcac cgtggccgac gcgatacgcc gaaccgccgg
     1801 ctcgggagca tggccatgca cccccaggag cttcgcgaaa tcattcccgg accccgcggg
     1861 cacgacgccc agcgccgctc gcccgcccgc cgaaagcagg ccgttcgcga cctcgtgcac
     1921 gatgccgtcc ccgccgaccg cgatcacgcg ctccgcgccc gcgcgcgaga actcagcggc
     1981 aagcgtggat ccgtgccccg gaccccgcgt atcgacggat tcgacggacc agccttcgcg
     2041 ctggaatgcc cgcgccaccc ggtccttgag ccgacgggcg gtgccccggc cggcggccgg
     2101 gttgacgacg agatgcgctg ttaggtgagg tatccctcgt cccacgcgac ctcgaactcc
     2161 cacttgcacg agggacactt gagtcgcttc gccttgccga gcttggaccg ttcgccgcag
     2221 gacgggcaga caccgtacgg atcgccgagg gacgcgcggg cgccggtctt gggcgcgcgc
     2281 accaccgtcc acttggggtg gggacggctc acgatctcga tcatggcccg gaggatggat
     2341 ttcggtttcc cgcccacctc gatcacgcac tcgagctcac cctcgtcgag gacccggtac
     2401 cacatgccgc gccgcaggcg gacgttcacg tcgtcccgca gccgggccca cttcgacttc
     2461 accccgatgg cgccacggct catcgcgtcc ctccagtgac gggtttccct ccggtgaagt
     2521 ccactacacg cgacgtgtac acgtagaagt tcgggtccac gtggaacgca tcgggtgacg
     2581 agagatgcac cgcggcctgt tgcagcccgg cagccggatg aatgagcgtg tagctgccgt
     2641 cggagagggt cacccggatc ggcatgtcga agcgcggaac gacgtccgcc caccggtacg
     2701 tgagcgtgga gtcgacgatt ttgtactcga agaccgggat catcgtcgtc cgcaagtact
     2761 cgtcgaacac tgtcgacaag ttcatcccgg cccgggtact gatgtagttc tcgatgtcct
     2821 gcgtcgtgac ggtctggtga taaaacgtgt gactcagccc gcgcaggatg tcccgccact
     2881 tgtcatcgtc gctcaccagc tgacggatgg tgtgcagcag gttgccgccc ttgtagtaca
     2941 tgtcgccgga tccgtcgcgg ttcacgccgt agggtccgat gatcggcgcg tcgttccgca
     3001 cctggaagcg cgtgccgatc acgtagtcgg cacccgcttc cttcccgcgc aagcactcca
     3061 cgaagaggtt ctccgagtag ttggcgaagc cctcgtggat ccacatgtcc gcctggtcct
     3121 tggccgtgat gctgttgccg aaccactcgt gcgcggattc gtggacgatg atgaagtccc
     3181 agttcgaccc ctggcctgaa cgtgacagat cgatgccggt tccggtcgcc gcggagtagt
     3241 agccgtcctt gtagtgattg ccgtacgcaa cggcgctctg gtgctccatg ccgaggtgcg
     3301 gcgtttccac cagctgatac ccgtccttgt accatgggta cgggccgaac cagtgctcga
     3361 aacacttcat catggtgacg gactgctgaa actggacctt cgcggtatcg aggtgatacg
     3421 cgagcggcca gaagttcatc gtgagcttgc cggactcacc cgcgtagtcc tgttcgaagt
     3481 gagcgtagac cgcggcgttc acggcgacgt cgtagttgtt gatcggttcc gtcacgaacc
     3541 actcgtacgt ggtcgtgtga tccttgttgt gcgtcgtgct ccggagacgg ccgttggaga
     3601 cgtcctgcag cggatcgggt accgtgatgg cgatccgctg gctgtcgggc tcgtcggcga
     3661 gatagtcctt gttcggccac cagacgctgg cgccgaggcc ctcgcaggcc gtcgcgatcc
     3721 agtcacggcc gagggagtct ttggcgaaga cgaacccgcc atcccagggc gcgcggcggg
     3781 cgacgcgcac ccggccgtgg taatagaccg ataccgtctt caccgatccg ctcggctgag
     3841 gtgccgtgag cgtcacgtag aacgcgttcc cgtcccggcg gtacgtcacc ttcttcccat
     3901 cctggagcat gctgtcgacc tggagcggtt cctggaggtc gatctgcatc tctttcgacg
     3961 ggcccgtgat ccggtacgtg atgccgttcc aacccttgat gctcgaatcg gatgggctca
     4021 ccgccacatg caggtcgtag aacgtgacgt cccaccaggc ccgttcgggc gtgttggagc
     4081 cgcggatggt atcggcatgg gtgaagactt cgcgctcagg ccgctgggca gaaaggctga
     4141 gaggcaacgc ggcgacggca aggccggcga ggagcaggtg gtggcgcaag agaggctccc
     4201 aaggggttca agtcaggggc gcgggggcac agaatggcat cccggggccc ctgaggcaag
     4261 agcatccaag tccttacatg ctaggccttt cgctcttcca gaccctgcaa cgccagaatc
     4321 aacgccagga gctggcagat ggcgccgagg aggaccatcc cgaacgacag gccgaccgca
     4381 ggactggcgt gctgggtgat cacacggacg acgcctgccg cactggcgaa cgtcggaatg
     4441 cccacccagg cagcgaggaa cacggcggct ttcggcaccc atcgtgcccg ctgccaagcc
     4501 cct
//
LOCUS       DASUBP010000137         5067 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_44583, whole genome shotgun sequence.
ACCESSION   DASUBP010000137 DASUBP010000000
VERSION     DASUBP010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5067)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5067)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5067
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44583"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="VFU75_05060"
     CDS             <1..784
                     /locus_tag="VFU75_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466236.1"
                     /translation="TDCVACHQPTFVATRNPDHVAGGFPTHCIECHNTTTWLASTFNH
                     QATRFPLTGAHLAVACNVCHADKVYMGKSTACASCHQADYNTTTDPSHTAAQLPSDCS
                     QCHTTASWSGGTFNHSTTKFALTGAHIPLDCNSCHGDGVYAGKGTTCVTCHLNDYNAT
                     NNPGHQGAGFPTGCATCHTTATWAGATFNHDGPYFPIYSGTHAGRWSTCTQCHTNPSN
                     YAVFDCLSCHQKTETDSHHTQVTNYQYLSTACYACHRNGQGG"
     gene            781..2328
                     /locus_tag="VFU75_05065"
     CDS             781..2328
                     /locus_tag="VFU75_05065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466237.1"
                     /translation="MKHILLGALLAPALAAGQARVVTARFAAVTYLTTTTAYLNAGRA
                     DGLRDSVRLEVFRNGQRVGAIRVAFLASHQASCDIIDTVRALAIGDSARYVPAAGPAV
                     TTAAAPVGWSAASRSAPPLLTGTVGLEYFSVSGDGVHLSQPGVDLRLLAGPPGSQWGG
                     VLDLRERRATTAAPGTSTSAVTESRVYQAFMRWRPGGASLRVEMGRQFVEGLSSVGLV
                     DGLQIVRETPTWAVGGFVGTQPGLADLEVSSDIRMVGAFVRRQGQPAGGGVWSLTAGA
                     SGSYQIWRTNREFMYFEARYFSPRFSGFLSQEVDYYRPWKRVGGESAVSPTATFGQLR
                     FEASPGLAIEGGVDERRNVRLFEDVVSPESTFDAAFRRGAWIGTSLRPGEHVWVELDA
                     HASDGGTIGRTDSYTASVSLVRLGALGANARTRATYYLSPGRVGRLAAMTLGVAPADR
                     WRVSVDLGQRDETVAILSSPQVSRWVGLSLDVSLARAWFMTFSATRQRGAQQDADQLF
                     AYLSYRL"
     gene            complement(2312..4252)
                     /locus_tag="VFU75_05070"
     CDS             complement(2312..4252)
                     /locus_tag="VFU75_05070"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU5466238.1"
                     /translation="MLLRDFRNFIAVAETRSLSRAAARAHLAQPALSRQIHELERELG
                     VPLLERHSKGVTPTAAGQAFARGAANLLVTLGFAIDRAEATSEGRRGRVSIGAMLTII
                     AAGIPAAVEERVHQEDPDITLAVEDLDPPDTVEQVRAGAIDIALAMAVGDDPLLAVEP
                     LWDEYLDHIILHHGHPLAGRRRLKLSDLADYPLIIPQRALAPGVLDEIMAVLREGGLR
                     SPLLVVDVGLRDGHLAVSAGRGWSLIGRARSVSPPQGTSVVALAGAAPKVRAAAIWRR
                     DDKRLVLRRVLEHILDAVRDHPHACLRREPHLPPSAPARKARRAPLGSVPTAMEIRHL
                     RALLEVAAAQTIGRAAERLGVTQPALSRQLKELEHATGLSLLERTARGVLLTPAGNSL
                     AGECPGILSGLEQIVLDTTRARRGMEGRCVIGAVATVTTGEMLARVLTEVAQRHPHVH
                     VVIEEVSTPLQAPAIVRGDIDLGLAHAHPGVAHPGLIVEHMFDDRLQMALLPAGHPLA
                     RRARLKPAELADLPFLFPDRDFQPAFYDRVFDALHDAGLIPRVDGTHAGLQAIWALVA
                     RGKGWGLGFKSHRRQPPGGTVAVPLVGFDLPWGLDLLRRRGEPSIALRCVLDAFRRAA
                     RMGARPSSGARRVRRRRVRADN"
     gene            4444..5016
                     /locus_tag="VFU75_05075"
     CDS             4444..5016
                     /locus_tag="VFU75_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466239.1"
                     /translation="MANTASRAASGALSRSLRRPVSVTVPEFAVSLLEQLPAVLGQGA
                     RPLVAVKAQLLGDISGTVAWVMTRTEAARLAGILLARESGSVALHDMATQAVLTRSAH
                     TLAGAYADVLAALTHGVVFLSVPEMVEGTIERLLSRRQVRPEPRQSNQLTVCVGSRIG
                     FDDQNLAVMGHLVFLPHHPTFGRILRALAD"
BASE COUNT          798 a         1777 c         1705 g          787 t
ORIGIN      
        1 cacggattgc gtcgcgtgcc atcagccgac cttcgtggcg accaggaacc ccgaccatgt
       61 cgcgggtgga ttccccacgc attgcatcga atgtcacaac acgaccacct ggctcgcgtc
      121 cacgttcaat caccaggcga cacggtttcc actgaccggc gctcacctgg cggtggcctg
      181 caacgtgtgt cacgccgaca aggtgtacat ggggaagagc acggcctgcg cgagctgcca
      241 tcaagcggat tacaacacta cgaccgatcc cagccatacc gcggcgcagc tgccgtcgga
      301 ctgcagccag tgccatacga ccgcgagctg gagcggcggc acgttcaatc actccacgac
      361 caagttcgcg ctgacgggcg cgcacatccc gctggactgc aattcgtgcc acggggacgg
      421 ggtgtacgcc gggaagggca ccacgtgcgt cacctgccac ctcaacgact acaatgcgac
      481 caacaacccg ggccaccagg gcgcggggtt cccgacgggc tgtgcgacgt gccacacgac
      541 ggcaacctgg gcgggggcga ccttcaatca tgacgggccc tacttcccca tctactccgg
      601 cacccacgcc ggccgctgga gcacttgcac ccagtgccac acgaatccgt cgaactacgc
      661 cgtcttcgac tgcctctctt gccaccagaa gacggaaacc gacagtcacc acacgcaggt
      721 tacgaactac cagtatctga gcacggcgtg ctacgcctgc caccggaacg gccagggcgg
      781 atgaagcaca tcctcctcgg ggcactgttg gcgcccgcgc tggcggccgg tcaggcccgg
      841 gtcgtcaccg cccgcttcgc ggccgtgacc tacctgacca ccaccacggc gtacctgaac
      901 gcgggccgag cggacggact gcgggattcg gtgcggctgg aggtgttccg caacggccag
      961 cgggtcggcg cgatccgggt ggcgttcctc gcctcgcacc aggcctcctg cgacatcatc
     1021 gataccgtgc gggccctcgc catcggggac tcggcccgat atgtcccggc ggcgggtccc
     1081 gccgtgacca ccgccgctgc accagttggg tggagcgcgg cctcccgctc cgccccgccc
     1141 ctgctcaccg gcaccgtggg cctcgaatac ttctcggtgt cgggggacgg ggttcatctg
     1201 tcacagcccg gcgtcgatct ccggctcctt gccggtccgc cggggtcgca gtggggcggc
     1261 gtgctggact tgcgggaacg ccgtgccacc acagccgccc ccggaacctc cacgtccgct
     1321 gtgaccgaat cgcgggtcta ccaggccttc atgcgctggc gcccgggtgg ggcgtcgctt
     1381 cgcgtcgaaa tgggccgtca gttcgtggag ggcttgagca gtgtagggct cgtggacggc
     1441 ctccagatcg tccgggaaac accgacctgg gcggtgggcg gcttcgtggg aactcaaccg
     1501 ggcttggccg atctcgaggt gtcgagtgac atccggatgg tcggcgcctt cgtgcgacgg
     1561 caaggacagc cggccggcgg cggggtctgg tccctcaccg cgggcgccag cggatcctat
     1621 cagatctggc ggaccaaccg cgagttcatg tacttcgaag cgcggtattt cagcccccga
     1681 ttctcagggt ttctctcgca ggaagtggac tactatcgcc cctggaagcg tgttggtgga
     1741 gaatccgccg tctctcccac cgccacgttc gggcaattgc gtttcgaggc ctcccccggc
     1801 ctggccatcg aagggggcgt ggacgagcgc cggaatgtcc ggttattcga agatgtggtg
     1861 agtccggaga gcacgttcga cgctgcgttc cggcggggag cctggatcgg cacgtccctg
     1921 cggcccggcg agcacgtgtg ggtggagctc gacgcgcacg ccagtgacgg tgggacgatc
     1981 gggcgcaccg actcgtacac cgcgtcggta tcgctcgtcc gactgggagc gctgggcgcg
     2041 aacgccagga ccagagccac ttactacctc agtccgggac gggtcggtcg actggcggcc
     2101 atgacccttg gggttgcccc cgctgaccgg tggcgcgtgt ccgtcgacct gggtcagcgc
     2161 gatgaaacgg tcgcgatcct ttcgtcgccg caggtcagcc gatgggtggg cctcagtctg
     2221 gacgtgagtc ttgcccgggc ctggttcatg acgttctcgg cgacgcggca gcgcggagcc
     2281 cagcaagacg ccgatcagtt gtttgcgtac ctcagttatc ggctctgacc cgccgccgcc
     2341 gaactcgacg agcgccggat gaggggcgcg cgcccatgcg tgccgcccgc cggaacgcat
     2401 ccaacacaca tcgcaacgcg atactcggct ccccgcgacg ccgaagcagg tcgagccccc
     2461 acggcagatc gaaccccacc agcggtaccg cgaccgtgcc acctggcggc tgccggcgat
     2521 gagacttgaa gcccagaccc caccctttcc cccgtgccac cagcgcccag atggcttgga
     2581 gtccggcgtg cgtcccgtcc acccgcggaa tcaaccccgc atcgtgcagt gcgtcgaaga
     2641 cccggtcata gaacgccggc tggaagtcgc ggtcgggaaa caggaacggc aggtcggcca
     2701 gctccgcagg cttcagacgt gcccgccggg ccagcgggtg accggcgggc aagagcgcca
     2761 tctgcaagcg gtcatcgaac atgtgctcga cgatcaggcc ggggtgcgcg actcccggat
     2821 gcgcgtgggc caggccgagg tcgatgtccc cgcgaacgat ggcgggagcc tgcaaggggg
     2881 tcgaaacctc ctcgatgacg acgtgcacgt gaggatgccg ttgcgcgacc tccgtgagca
     2941 cgcgcgcgag catctcgccc gtcgtgaccg tggccacggc accgatgacg caccgccctt
     3001 ccatgcccct ccgggcgcgg gtcgtgtcga ggacgatctg ctcgagcccc gacagaattc
     3061 cggggcattc ccccgccagc gaattccccg ccggagtcag cagcacgccc cgcgccgtcc
     3121 gctccagaag cgaaagaccg gtcgcgtgct cgagctcctt gagctggcgg gagagcgccg
     3181 gctgggtgac gccgaggcgt tcggcggccc ggccgatcgt ctgtgcggcc gcaacctcga
     3241 gcaatgcccg gagatggcgg atttccatcg ccgtcgggac tgagccgagg ggcgcccgtc
     3301 tggctttccg cgccggagcc gacggcggga gatggggctc acggcggagg cacgcgtggg
     3361 ggtggtcgcg gacggcgtcg aggatatgct cgagcacccg ccgcaggacg aggcgtttgt
     3421 cgtcacgtcg ccagatggcc gccgcccgga ccttgggcgc cgcgcccgcc agggcgacga
     3481 cggacgtccc ctgcggcggc gataccgacc gggcccggcc gataagcgac cacccacgtc
     3541 ccgccgacac cgccaggtga ccatcgcgca agccgacgtc gaccacgagc aagggtgacc
     3601 gcaacccgcc ttcccgcagc accgccatga tctcgtcgag caccccgggc gccagcgcgc
     3661 gctggggaat gatgagcgga tagtcggcga ggtccgagag cttgagccgc cgacggccgg
     3721 cgagcgggtg tccgtggtga agaatgatgt gatcgagata ctcgtcccac agcggctcaa
     3781 cggcaagtaa cgggtcatcc cccaccgcca tggccaacgc gatgtcgatg gcacccgccc
     3841 gaacctgttc cacggtgtcc ggggggtcga gatcctcgac ggcgagcgtg atgtccgggt
     3901 cctcctgatg gacccgttcc tccaccgccg ccggaatccc ggccgcgatg atggtgagca
     3961 tcgccccgat ggaaacgcgt ccccgccggc cttcgctggt cgcctccgcg cggtcgatgg
     4021 cgaagcccag cgtgacgagg aggttggcag cccctcgcgc aaaggcttgc ccggcggcgg
     4081 tgggcgtgac gcccttggaa tgccgttcca gcagggggac gccgagttcg cgctcgagct
     4141 catggatctg ccgggaaagg gccggctggg cgaggtgggc gcgtgccgcg gcacgggaga
     4201 ggctccgcgt ctcggcgacg gcgatgaagt tccggaaatc ccgcaggagc atcggacccc
     4261 ttggccatgc gccgacggca tcgaagccct agcggatcgg tatttcctgc cggcctgtct
     4321 actccgctag gatgaccgtc gacgatttcg cgcccctcca ggcgcaggag tgaccatacg
     4381 ttcgctgaaa cactgccgct agaggcctgc gagccggctc gccatctggg cgcgctgcgc
     4441 caagtggcca ataccgccag ccgcgcggcc tccggggcgc tctcccggtc tctgcgccgc
     4501 cccgtctcgg tcaccgttcc ggagttcgcg gtttccctgc tggaacagct tcccgcggtc
     4561 ctggggcagg gtgcgcgccc gctcgtcgcc gtgaaggccc agctcctggg agacatttcc
     4621 ggtaccgtcg cgtgggtcat gacacgcacg gaggcggccc ggttggcagg cattctcctg
     4681 gcccgcgagt ccggatccgt cgcgctccac gacatggcga cgcaggcggt gctgacgcgg
     4741 agcgcgcaca cgctcgccgg cgcctacgcg gacgtattgg cggcgttgac gcacggcgtg
     4801 gtctttctct ccgtacccga gatggtagaa ggcaccatcg agcgcctgct gtcccgacgg
     4861 caggtcaggc cggaaccgcg ccaatccaat caattgaccg tgtgcgtcgg gtctcgcatc
     4921 ggttttgacg accagaatct cgcggtgatg gggcacctcg tattcctgcc gcaccatccg
     4981 accttcggcc gcattcttcg cgcgctggct gactgatgac ccgacggttt cccctgcccc
     5041 cctcgccggc gggcattccc gcatgcg
//
LOCUS       DASUBP010000138         3606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_916858, whole genome shotgun sequence.
ACCESSION   DASUBP010000138 DASUBP010000000
VERSION     DASUBP010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_916858"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1543
                     /locus_tag="VFU75_05080"
     CDS             <1..1543
                     /locus_tag="VFU75_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5466240.1"
                     /translation="IASTIGSRIADNERRRVAESEARFRALFEGAGDPIAALEPDGRV
                     HDANPRLLALLGRPLEDVRGKLLAHLLAPGRGDGWLLAPLEAATQGAVTHVERIKRAN
                     GPELELEVTLARVDTPAGPLVQVILHDRTERRALERRQVQAERLDGLARFAGGIAHQF
                     NNLMGGVMTHASLLRTEANEAGIQAVDEILAAARRGRDLTKELLRFTKAGELLLRSTQ
                     VKEILDRVTLLARSALPESIQLRVEADPGLPNVHADPDQLSHAIYQLILNARDAMRDQ
                     PSGRVTITAGRETVERDPRWPEAAPGSYVRISVNDIGAGMDATTVERVLEPFFSTKPM
                     HQAAGLGLAAVYGVVRDHQGSVRISSALGRGTTVDLLIPRSTAPEPEPVRPTPAASVP
                     PAPSMVILVVDDEAIVRSSLKRALTKFGHQVLEAEDGPTALRAMQTANPPVQLVILDL
                     VLPGGGAGILELLKATQPTVKVLVSSGYSPDHEVVKGIENRADGFLQKPFEIADLRKA
                     IAKFQ"
     gene            complement(1598..2587)
                     /locus_tag="VFU75_05085"
     CDS             complement(1598..2587)
                     /locus_tag="VFU75_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEU5466241.1"
                     /translation="MKVIVPLAGKGTRLLPLTKIVPKPLVEVAGRPVMDYVMDLIKNA
                     GLDVDELIVITGHLKEAVEKYIVSHYGVAARFVEQKTLDGTAGAINLARPYVDGPVLI
                     IFVDTLFDADLSLIKRTDADGIIWAKEVEDYQRFGVVVHDAQGFMTKIVEKPDTPVSK
                     LANIGLYYIRDWKTLFDGIAQVLASPANKGEFYLTDAFQYMVDRGKKILTAEVQGWYD
                     CGKVDTLLETNAHVLKTGRARKPAIPAGVTIKEPVYIEDGVTLKDSTIGPNVSLGAGS
                     VVERSTLENSILGRRVRVSDCTVSGSVIGNDQVLYGSQLTNMVASGGEVAKAR"
     gene            complement(2587..2937)
                     /locus_tag="VFU75_05090"
     CDS             complement(2587..2937)
                     /locus_tag="VFU75_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5466242.1"
                     /translation="MNDVPYRVEKPWGHELIWARTDRYVGKVLHIEPGQVLSLQYHKM
                     KDETIHVLTGEIILRTKPGDAMLERRMKPGESFHIEPPLIHQFEAVVATDLLEVSTPE
                     IDDVVRLQDRYGRA"
     gene            complement(2934..3446)
                     /locus_tag="VFU75_05095"
     CDS             complement(2934..3446)
                     /locus_tag="VFU75_05095"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HEU5466243.1"
                     /translation="MTARTERRVAATRYFRPPGAGPELAFLAACTRCGACIDVCPVHA
                     IIKLPAGEGLAAGTPVLEPSRRACVACADMPCAAACPTTALQVPADGWASIHLGRLAL
                     DPERCITFHGSACGVCARACPVGERALAMDAAGHPVLKPEGCVGCGLCVTACVTYPSS
                     LTLRLPEELS"
BASE COUNT          626 a         1218 c         1164 g          598 t
ORIGIN      
        1 catcgccagc accatcgggt ctcgcatcgc ggacaacgag cggaggcgtg tcgcggagtc
       61 cgaggcccgg tttcgagcgc tgttcgaggg cgccggcgac ccgatcgccg ctctcgaacc
      121 cgatggccgg gtacatgatg caaacccgcg cctcctcgct ctgctgggaa gaccactcga
      181 ggacgtgcgg ggaaaactcc tcgcgcacct gctggcgccg ggacgagggg atgggtggct
      241 cctcgctccc ttggaagccg cgacgcaggg tgcggtgact cacgtcgagc ggatcaagcg
      301 ggccaacggc ccggaactcg agctggaagt gacgctcgcg cgcgtcgata ctccggcggg
      361 accgctggtc caggtgatcc tccacgaccg caccgaacgt cgcgccctcg aacgtcgcca
      421 ggtccaggcc gaacggctcg acgggctggc acgatttgcg ggcggcatcg cgcatcagtt
      481 caacaacctc atgggcggcg tgatgacgca cgcctcgctc ctgcgcacgg aagcgaacga
      541 ggcgggtatt caggcggtgg acgagatcct ggcggccgcg cgccgcggcc gggacctcac
      601 caaagagttg ctgcgcttca cgaaggccgg tgagctgttg ttgcgttcga cccaggtgaa
      661 ggagatcctc gaccgcgtca cgctcctcgc gcggtccgcg ctccccgaat cgattcagct
      721 ccgggtcgag gccgaccccg gactgcccaa cgtccacgcc gatcctgacc agctctcaca
      781 cgccatctat cagctcatcc tcaacgcgcg cgacgccatg cgggatcagc cgtcaggacg
      841 ggtcaccatc accgcgggcc gggaaaccgt cgagcgggat ccgcggtggc cggaagcagc
      901 gcccgggtcc tacgttcgga tttcggtgaa cgacatcggc gccgggatgg atgccacgac
      961 cgtcgagcgg gtgctcgagc ctttcttttc caccaagccc atgcatcagg cggccggact
     1021 cggcctcgcg gcggtctacg gcgtggtgcg ggaccaccag gggtcggtgc ggatctcgag
     1081 cgccctgggg cgcggcacga cggtggacct cctgattccc cggagcaccg ccccggaacc
     1141 ggagccggtc cggccgaccc ccgcagcgtc cgtacccccg gcccccagca tggtcattct
     1201 cgtggtggac gacgaagcga tcgtgcggtc atccctcaag cgtgcgctca ccaagttcgg
     1261 gcaccaggtg ctcgaagccg aggatggtcc gacggcactg agggccatgc agacggcgaa
     1321 cccgccggtg cagctcgtga ttctcgatct ggtcctgccg ggagggggcg cggggatcct
     1381 cgagctgctc aaggccacgc agcccaccgt caaagtcctc gtgtcctcag gctattcgcc
     1441 cgatcacgag gtggtcaaag ggatcgagaa ccgggccgat ggattcttgc agaaaccgtt
     1501 tgagatagcc gatctcagga aagcgatcgc caagtttcag tagaacgcta agtggcttag
     1561 cgacctagcc acttagcgac ttagcgaact ccacgtctca ccgagctttg gcaacctcac
     1621 cgccagaagc gaccatattc gtcaattggg agccatacag cacctggtcg tttccgatca
     1681 ccgagcctga aaccgtacag tcactcacgc gcacccggcg gccgaggatg ctgttctcga
     1741 gtgtcgagcg ctcgacgacg cttccggcgc ccagcgaaac gttggggccg atggtcgagt
     1801 ctttcagggt gacgccgtct tcgatgtaga ccggttcctt gatggtcacg cccgcgggaa
     1861 tcgccggctt ccgcgcccgg ccggtcttca ggacgtgcgc gttggtctcg agcagtgtat
     1921 cgaccttccc gcaatcgtac cacccttgca cttcggcggt caggatcttc ttcccccggt
     1981 ccaccatgta ctggaacgcg tcggtgaggt agaactcgcc cttgttggcg gggctcgcga
     2041 gcacctgcgc gatgccgtca aaaagggtct tccagtcgcg gatgtagtac agcccgatat
     2101 tcgcgagctt ggacaccgga gtatcgggct tctccacgat cttcgtcatg aacccctggg
     2161 cgtcgtggac caccacgccg aagcgctggt agtcctcgac ctccttggcc cagatgatcc
     2221 cgtcggcgtc ggtccgcttg atcagcgaga ggtcggcgtc gaagagcgtg tccacgaaga
     2281 tgatcaggac cggcccgtcc acgtagggac gggccagatt gatggcgccc gcggtgccgt
     2341 ccagcgtctt ctgctcgacg aacctcgcgg cgacgccgta gtgcgatacg atgtacttct
     2401 cgaccgcctc cttcaagtgt cccgtgatca cgatcagctc gtcgacgtcg agtccggcgt
     2461 tcttgatcag atccatcacg taatccatga cgggccggcc ggcgacctcg accagcggct
     2521 tcggaacgat cttggtgagc gggaggagcc gggttccctt tcccgcgagc gggacgatga
     2581 ctttcatcac gcccgcccgt agcggtcctg cagccggacg acgtcgtcga tttcgggggt
     2641 ggacacctcg aggagatccg tcgcgaccac cgcttcgaat tggtgaatca ggggcggctc
     2701 gatgtgaaag gactcaccgg gcttcatccg gcgttcgagc atcgcgtccc ccggcttggt
     2761 ccgcagaatg atctcgccgg tgagtacgtg aatggtctcg tccttcatct tgtgatactg
     2821 cagggagagc acctggcccg gctcgatgtg gaggaccttc ccgacgtaac ggtcggtccg
     2881 ggcccagatc agctcatggc cccacggctt ctccacccgg tacggcacgt cgttcacgaa
     2941 agctcctctg gcaaacgcaa agtcaatgaa gaaggatagg tgacacaggc cgtcacgcag
     3001 agcccgcacc ccacgcaccc ctccggcttg agcaccggat gccccgcggc atccatcgcg
     3061 agggcccgct ctcccaccgg gcacgcccgt gcgcacacac cgcacgcgct gccgtgaaac
     3121 gtgatgcacc gttccgggtc gagcgccagg cggcccaggt gaatgctcgc ccacccgtcg
     3181 gccggcacct gcagagccgt ggtgggacag gccgccgcgc agggcatgtc cgcgcaggcg
     3241 acgcaggcac gccgtgaagg ttcgaggact ggtgtgcccg ccgccagtcc ctcgccggcc
     3301 gggagcttga taatggcatg cacgggacag acgtcgatgc acgctccgca ccgcgtgcag
     3361 gccgcgagga aggccagctc gggccccgcg cccggaggac gaaagtaacg ggtcgccgcg
     3421 acccggcgtt cggttcgggc ggtcacctcg cgggccagct tcgcgaacgc gtcgcggaag
     3481 aaggcccgcc gttccggctg gtccagctta gaagctcggt gccaaggata ccgaccagcg
     3541 gaacgccgca ttcccgccgg accggacatc ttcggccctg tcgaaccggt tcacaaggaa
     3601 gggcac
//
LOCUS       DASUBP010000139        15450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_126597, whole genome shotgun sequence.
ACCESSION   DASUBP010000139 DASUBP010000000
VERSION     DASUBP010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 15450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15450
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126597"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..465
                     /locus_tag="VFU75_05100"
     CDS             <1..465
                     /locus_tag="VFU75_05100"
                     /inference="COORDINATES: protein motif:HMM:NF022072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HEU5466244.1"
                     /translation="MLTIDRIAVCAPFDRVFTVASDVQNWPEILAHYRWVRTLERRPN
                     GALVEMAAWRPFGPLKWPTWWVSEMTVNRPAREIRYRHVQGITSGMDVVWRMSSGSAG
                     VAIELEHRWTGPRWPVIGPVAATAVIGPVFVHGIASRTLAGLKAYLEKKGNA"
     gene            462..1703
                     /gene="fabF"
                     /locus_tag="VFU75_05105"
     CDS             462..1703
                     /gene="fabF"
                     /locus_tag="VFU75_05105"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU5466245.1"
                     /translation="MNEHRRVVITGVGAVTPIGTGAPGLWDGLQRRTSAVRTITRFDP
                     SPFRSHIAAEIPDFRPQDHLDAKRAKRLDRFSQLAVTSARMALEDARIDMAGEDPDRI
                     GTMMGSALGGVSYAESQVGNYLREGVKGMDVGLALSVFAGAASCNIAIEFGCTGPNAT
                     NAMSCASGTIAVGEAFHVVRDGRADIMLAGGAEAPLAPLTFGAFSNIRAMSTRNDDPA
                     HASRPFDATRDGFVMGEGAAVLILEERSRALARGAPIYAEVVGHAFTNDAYHMTAPRP
                     DGRQAARAMRLALADGGVTPGEIGYVNAHGSSTPLNDSTETSALKQVFGDHAYRLAVS
                     GTKGYYGHALGASGAIEAAICALAMSNGWLPPTINLERPDPACDLDCLPGAGRDGTPE
                     GVVSNSFGFGGINASLVLRRA"
     gene            1752..2432
                     /locus_tag="VFU75_05110"
     CDS             1752..2432
                     /locus_tag="VFU75_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEU5466246.1"
                     /translation="MNSQALRPLVAEFIGVALFVFIGAGSVVANGMTTGGLGAIGIAL
                     AHGLAMGLIVSATLSISGGHMNPAVTFALWVARKIDAKLAGLYVVAQLLGAIVGAALL
                     AFVFPKPSVDAMAYGTPLLSSLTTFDRGVAMEAILTFVLVSAVFGTIVSRQAPKIAGW
                     GVGVAILVDALVGGHTTGAIMNPARAFGPAIVSWTLTGHVVYWLGPLLGALVAALVWT
                     KILLPKDG"
     gene            2612..2974
                     /locus_tag="VFU75_05115"
     CDS             2612..2974
                     /locus_tag="VFU75_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466247.1"
                     /translation="MGAYRASRNAIVKFPHSADAWMLAGQGAELVFRQAEALTAYEQA
                     MVIEERADAALAAAALYVRAGRHADAAARFARGFAAGGGPEALRQNAESLARAGDTAA
                     AEEAMRLFRELSNRAGTR"
     gene            complement(3049..3708)
                     /gene="ccsA"
                     /locus_tag="VFU75_05120"
     CDS             complement(3049..3708)
                     /gene="ccsA"
                     /locus_tag="VFU75_05120"
                     /inference="COORDINATES: protein motif:HMM:NF013725.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HEU5466248.1"
                     /translation="MTLKTRGRAITLLGLVLLAGVYVRAIWFTPVERLQGGAQKIFYV
                     HVPAAWSTMLAFSLVGLASILYLLMKDERYDRFAAASAEVGTVFACVMLTTGPLWAKP
                     IWGTWWTWDARLTSTLFLFFLFIGYLLLRGAIPEAGTRARYSAVLGIFGAALVPFIHL
                     TVYLFRTLHPQPIVLKPSAPSLPGDMLATLLFSFLTFTILYVGFVTQRYALGRGDSHV
                     G"
     gene            complement(3705..4385)
                     /locus_tag="VFU75_05125"
     CDS             complement(3705..4385)
                     /locus_tag="VFU75_05125"
                     /inference="COORDINATES: protein motif:HMM:NF015344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HEU5466249.1"
                     /translation="MGEILRDAWVLARKDLALELRTKTGLASALVFTALVLTVFNFGR
                     DPTAVAALDLAPSILWVTFTFAAVLSLNRAFQLELENQALDGLLVSPVDRRALYLGKL
                     FANLCFVGFVEAVGLPLFALFFNISIVHVLLPLAALIAVATIGFVAVGTLLSALVIRV
                     RFAELMLPVLLLPFFIPPLVGAVQVTARLFSGRPLSEVSGWLRLLLAYDLVFVSLATL
                     LFPTTVDE"
     gene            complement(4385..5065)
                     /locus_tag="VFU75_05130"
     CDS             complement(4385..5065)
                     /locus_tag="VFU75_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5466250.1"
                     /translation="MLWADGVRHRFGGVPALDDVSFTLPAGHTLALFGPNGSGKTTLL
                     KVLAGLIKPNLGHAGVQGGRGAIGWIGHQSLLYGQLTVRENLRFWGSLYRVRTPVLDA
                     RIASLTARVDLREQLDRRVGTLSRGQTQRAAIARALIHDPSVLLLDEPFTGLDRVAAS
                     ELTALVKEFAGAGEGRATILVTHNVEEGTDLATDVAVLKTGRLVEFMPRNARTAEQIG
                     ARYRELVS"
     gene            complement(5052..5423)
                     /locus_tag="VFU75_05135"
     CDS             complement(5052..5423)
                     /locus_tag="VFU75_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5466251.1"
                     /translation="MPVEAPPRRRVLIVEDEAALRSSYARFFEARYEMAFARTGAEAL
                     RQLESFDPQVLILDLRLPDTDGIALLQKIRATHATLPVVVTTAYVSMEPLMKVLDLGH
                     SGYLVKPFDLNELAARIDAVG"
     gene            complement(5439..5759)
                     /locus_tag="VFU75_05140"
     CDS             complement(5439..5759)
                     /locus_tag="VFU75_05140"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU5466252.1"
                     /translation="MSSRSSEKTLAAPEALSLESRTAFRRTAVELLDQLPEGAGRLVI
                     DLSATRAVDSAGLGTLMLIQRRAAERRVAVALRGANEELRFLLVLTKLDDLFELEGGA
                     GSGG"
     gene            5948..7885
                     /gene="acs"
                     /locus_tag="VFU75_05145"
     CDS             5948..7885
                     /gene="acs"
                     /locus_tag="VFU75_05145"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HEU5466253.1"
                     /translation="MTDRIETLLDEQRTFPPPAGFTAQAHVRDASIYQKAKADREGYW
                     ADWARQLEWMKPWDRVLEWTPPHAKWFLGGKLNASVNCLDRHVKAGKRDKIGFIWEGE
                     PGEVRNITYGDLLEDVCRCANGLKSLGVRKGDRVAIYMPMVPEVAVAMLACARLGAVH
                     TVVFGGFSSESLRDRINDAQAKVLITADGGYRRGGQVPLKQNADDALTDTPSIQHVVV
                     LKRTGQAVSMKAGRDQWWDALVTPQSPECAPEPMDAEDPLYILYTSGTTGKPKGILHT
                     TGGYLTQVFATTKWVFDLKDSDVFWCTADVGWVTGHSYVVYGPLAVGVTQLMYEGAPD
                     HPSKDRFWALCAKHGVTVFYTAPTAIRSFMKWGTELPARHDLSKIRLLGSVGEPINPE
                     AWIWYHENIGGKRCPIVDTWWQTETGGIMITPLPGITTTKPGSGTVAFPGIDAVLLDG
                     DGKEVKVGGGYLAITSPWPGMLRTIWGDDDRYRNVYWSRWPKTYFPGDGAKRDDDGYF
                     WILGRVDDVLNVAGHRIGTMEVESALVDHPAVAEAAVVGRTHDLKGQALAAFVTIKEG
                     RQATDALREELKGHVAKKIGALARPDDIIYAADLPKTRSGKIMRRLLKDIAEGRALGD
                     TTTLADPAVVQRLKTLYEDKE"
     gene            complement(7919..9730)
                     /locus_tag="VFU75_05150"
     CDS             complement(7919..9730)
                     /locus_tag="VFU75_05150"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU5466254.1"
                     /translation="MTLWRSAKRVLVGRPLETLHLESERLSKATGLAVLSSDALSSVA
                     YAAEAMLIVLMTAGAAGVGRGLVVAGFILLLLTIVVVSYRQTVFAYPQGGGAYFVAKE
                     NLGTGFGLVAAAALLVDYTLTVTVSVAAGVAAITSAFPAAAPHAVALGIGAIALVAIG
                     NLRGLRESGMIFSVPTYFFIGMFALMLIVDVVHPFGTFTPATLPVSTGTAVTLWLLLK
                     AFASGCSALTGVEAISNGIQAFRPPESRNAATVMIWMGAILGVFFAGSTIIAHRFGVV
                     PVGDQTVISQLAHGAFGNGPFYYAVQFSTALILVLAANTAFADFPRLSSILATDGYLP
                     RQLASRGDRLVFSNGILMLALVAGVLLEVFGGNTNALIHLYAVGVFISFTLSQAGMVR
                     RSLKAGERRVGRLVASSLGTLATAVVVVIELVTKFTEGAWISALLIVALVFVFLAIKR
                     HYQFYARQLSSDAWQPEWPDKHLVVVPVAGVSKSTANALAYALLLSPHIRAVAVATDP
                     KQVQRMQDEWDLWDSGVPLEVVVSPYRAIVSPLLQFIDQVERTEKPDWLTVVLPEVVP
                     ARWWHAFLHNQTVYAIKTALLFRKNVVVTTVRHHLRK"
     gene            complement(9741..10163)
                     /locus_tag="VFU75_05155"
     CDS             complement(9741..10163)
                     /locus_tag="VFU75_05155"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HEU5466255.1"
                     /translation="MSLHRQAVAVLALVGIAIATYLWLHAIGVVGALKCGTGGCEIVQ
                     ASSYARLFGIPVAFYGVVGYVTLFGISIVGSQPAMVSRAWPTRLLLVLSGAGFLFTMY
                     LTSLEAFVIHAWCRWCLASAAVITAIFVTSLAGSLRKS"
     gene            complement(10160..10846)
                     /locus_tag="VFU75_05160"
     CDS             complement(10160..10846)
                     /locus_tag="VFU75_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HEU5466256.1"
                     /translation="MKRFYGLLAVVAVAAIAFLIYTANQPASAVSGSAGSRAPVTAQD
                     SAFPGYVLGSDSAPVKVVEFADYECPICAMFATVQFPTIRDQLIKTGKVQWTLRDYPL
                     PPTTHRWSHLAANAVACASEQGKAWDMIDAVFNHHDWALQSSNPTSVFRGLAQGVGVN
                     TTTWDACVSADRYAGRIQASLDRGDALGVSGTPTFFIDGKEVARGDRSSDEFQRLADS
                     IIAAHPKKKK"
     gene            complement(10843..11157)
                     /locus_tag="VFU75_05165"
     CDS             complement(10843..11157)
                     /locus_tag="VFU75_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466257.1"
                     /translation="MSDHDETYGPERAERLAALLRALAARIAELDRTGRLLESAPELQ
                     ELLGDARSELFHYEVRSTYDTPEVAESRRIVEEAERRFSELRFDATDDEDDQPWRRSG
                     GE"
     gene            11276..11548
                     /locus_tag="VFU75_05170"
     CDS             11276..11548
                     /locus_tag="VFU75_05170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466258.1"
                     /translation="MAKARTDTRKAPPTPTSFEQARDELFSHILRCGVLEATSEHQKE
                     WFDDTMLYLGDRYEDLSEEEMTQLRVLGERYCKPVMARAAPAVAST"
     gene            complement(11607..12071)
                     /locus_tag="VFU75_05175"
     CDS             complement(11607..12071)
                     /locus_tag="VFU75_05175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466259.1"
                     /translation="MWWSKLAALSVCGAAVATAQGPPASETSLGAAALWGRRPYYGLD
                     AGLGLRPAGQVRFTMTAGAGVRDGRPAGRVGGEAQFLVLPAAKSGVSPYVGMGVNCLM
                     VDSRHSFGALTLTVGVEAAEGNRFGWYVEGGASGGARLAVGVRWRRFPAWWR"
     gene            complement(12058..12471)
                     /locus_tag="VFU75_05180"
     CDS             complement(12058..12471)
                     /locus_tag="VFU75_05180"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEU5466260.1"
                     /translation="MAAPQATIGLRWLGEGLVFEGTGQSGKPTLVDGDTKVATSPVEA
                     LLVAAASCTASDVVIILKKMRVDLTKLEIRLTGTRRETEPRRVTAMVLHVRVKGAGAD
                     ESKVRTALDLSIEKYCSVITSLNPDIPVTYDIVVE"
     gene            complement(12472..13008)
                     /locus_tag="VFU75_05185"
     CDS             complement(12472..13008)
                     /locus_tag="VFU75_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466261.1"
                     /translation="MSRLKVIITAGVLSVLPGAGFAQQFTPPQGASTPSTGTRLGLFG
                     FGVRGGIDVSHGGGQMVLGTTLDVGDLFTPQVRIRPSAEIGVFNGGNTYVGSLEALWR
                     FTDDNQTAIPYVGAGVSLAGHQACGADPSCPAVWVNLVLGFELHYRSSFNWLLEYHGM
                     DALRRHRFYIGLTTRRGG"
     gene            complement(13005..13517)
                     /locus_tag="VFU75_05190"
     CDS             complement(13005..13517)
                     /locus_tag="VFU75_05190"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEU5466262.1"
                     /translation="MKPWFTPADALTSLRLPLAVAFPLVTGMPLRLAIIGVAAASDFF
                     DGRVARRYGGSRAGAILDPVADKVFMAAAFLTLWRSGPLVWWEIAGVLLRDIFAAGGG
                     LAVWLFHRPVPFPARAGGKAVTVGQILTLVAFVVESPLVRPLAWATAAISVYALWDYG
                     RALRPRTEDT"
     gene            13553..14011
                     /locus_tag="VFU75_05195"
     CDS             13553..14011
                     /locus_tag="VFU75_05195"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HEU5466263.1"
                     /translation="MDLNLLKQAAIFQDLDEGELARVSEVCREQKFTVGQYIFKEGEP
                     GNRLFIIAEGEVRISRTIPGSGEEALAVLKPGANFGEMAIFDRSERSTDAIANISCTL
                     ITISRSDFELLLDFNRDIAYKVLWSVVRLLCGRLRVTNDNLRSFLAMSMF"
     gene            14152..15123
                     /locus_tag="VFU75_05200"
     CDS             14152..15123
                     /locus_tag="VFU75_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466264.1"
                     /translation="MGDSGAALTATYYNGNGSVGTSGPKNWSSSDSTVVLVDASSGAL
                     KAVGSGTAVLTVTASGVTGQALVVVTDTLDLALLLPQIVLMPQDTFVVPVSIRNKSGG
                     AIAPPWFSASVNGYFTVDSATGRVVSTGTGAPAPYLAHLGALTANGTVQVVSMADTTG
                     GGAAYTVNGSVSAQRSASTVRAQNYTRAGGTPTFLLTLKVVVNGVATELVNVLSQTAV
                     NGGPDSLGLDSVSVTEAESNSFLCSPPRSAAAWTSTVGGNPILAVSRPGGYVKIRQET
                     TVTGGHAIAGSFYFNGQRADDYTDPSGVLAIRGDFVAPLITVTNTCK"
     gene            15131..>15450
                     /locus_tag="VFU75_05205"
     CDS             15131..>15450
                     /locus_tag="VFU75_05205"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU5466265.1"
                     /translation="MNRLTPYAAVLLRIAVGLIFLHHGIQKLHMGAAGVAGFFHGAGI
                     PFSTVFAYIVIALETVGAVLLILGVIPRIIAIGYVIEMLVAIFVVILPSHPQLPELEA
                     LLCAG"
BASE COUNT         2758 a         5234 c         5099 g         2359 t
ORIGIN      
        1 atgttgacca tcgaccgaat tgcggtctgc gcgccgttcg accgagtctt caccgttgcc
       61 tcggacgtgc agaactggcc cgagatcctc gcgcattacc ggtgggtccg gacgctcgag
      121 cgccggccga atggcgccct cgtggaaatg gcggcgtggc gtccgttcgg tccgctcaag
      181 tggcccacct ggtgggtctc cgagatgacg gtcaaccggc cggcccgtga aatccggtac
      241 cggcacgttc agggcatcac ctcgggaatg gacgtcgtat ggcggatgtc gtccgggtcg
      301 gccggcgtcg ccatcgagct ggaacaccgg tggacgggac cccgatggcc ggtcatcgga
      361 ccggtcgccg ccaccgcagt gatcgggccc gtgttcgttc acggcatcgc ttcgcgaacg
      421 ctcgcgggtc tcaaggccta tttggagaag aagggaaacg cgtgaacgaa catcgtcgtg
      481 tcgtcatcac cggcgtcggc gccgtaacgc cgatcggcac gggcgcgccc gggctctggg
      541 acggcctcca gcgccgcacg tccgcggtac gcaccatcac ccgcttcgac ccgtccccgt
      601 tccgcagcca catcgcggcc gagattcccg atttccggcc gcaggaccat ctcgacgcca
      661 agcgggcgaa gcggctcgac cggttctcgc agctcgccgt gaccagcgca cgcatggcgc
      721 tggaagacgc ccggatcgac atggcgggcg aagaccccga ccgcatcggc accatgatgg
      781 gctccgccct gggcggcgtg tcgtacgccg agtcgcaggt cgggaattac ttgcgcgagg
      841 gcgtgaaggg catggatgtc gggctggcgc tgtcggtctt cgccggcgcc gcgagctgca
      901 acatcgcgat cgagttcgga tgcacgggtc ccaacgccac caacgcgatg agctgcgcgt
      961 ccgggacgat cgcggtgggt gaggcgttcc acgtggtgcg cgacggccgg gccgacatca
     1021 tgctggccgg tggagccgag gcacccctgg ctcccctcac gttcggcgcg ttctcgaaca
     1081 tccgggcgat gagcacgcga aacgacgatc cggcccacgc ctcacgtccc ttcgacgcga
     1141 ctcgcgacgg attcgtgatg ggcgaagggg cggccgtgct gatcctggag gagcggagcc
     1201 gggcgctggc gcggggcgcg ccgatctacg ccgaggtggt gggacacgcc ttcacgaacg
     1261 acgcgtatca catgacggcg ccgcgtccgg acggccgcca ggcggcgcgg gccatgcggc
     1321 tcgcgctcgc ggacggtggt gtgacgcccg gcgagatcgg gtacgtcaac gcgcacggct
     1381 cttccactcc cttgaacgat tccaccgaaa cctccgccct caagcaggta ttcggcgatc
     1441 acgcctaccg gctggcggtg agcggcacga agggctatta cggccacgcg ttgggcgcga
     1501 gcggtgccat cgaagcggcc atctgcgcgc tggccatgtc gaacgggtgg ctccctccca
     1561 ccatcaacct cgagcgtccc gatccggcgt gtgacctcga ttgccttccc ggcgcgggcc
     1621 gtgacggaac gcctgagggc gtggtctcca actcgttcgg cttcggcggc atcaacgcgt
     1681 cactggtgtt gcggcgggcg tagtcccgtc ggtagattgg cgccgcttcc ctcctcccaa
     1741 aggacgccct tgtgaattcg caagccctgc ggcccctggt ggccgagttc atcggtgtgg
     1801 cgcttttcgt cttcatcggc gccggcagcg tggtcgcgaa cggcatgacc accggcggcc
     1861 tcggggccat cgggatcgcg ctggcgcacg ggctggcgat gggtctgatc gtcagcgcca
     1921 ccctgtcgat ctccggcggc cacatgaatc cggcggtgac gttcgcactg tgggtcgcga
     1981 ggaaaatcga tgccaagctc gccgggctgt atgtggtggc ccagctcctt ggcgcgattg
     2041 tgggggccgc actcctcgcc ttcgtgttcc cgaaaccgtc cgtggatgcg atggcgtacg
     2101 gcacgccgct cctttcgtcc ctcaccacat ttgatcgcgg cgtcgcgatg gaggcgatcc
     2161 tcaccttcgt cctggtgagc gcggtgttcg gcacgatcgt gtcgcggcag gcaccgaaga
     2221 tcgccggctg gggtgtcggt gtcgcaattc tggtcgacgc gctggtcggt ggtcacacga
     2281 cgggagcaat catgaatccc gcccgcgcct tcgggcccgc catcgtctcc tggacgctga
     2341 cgggtcatgt ggtctactgg ttggggcccc tgctcggcgc cctggtcgcg gccctggtgt
     2401 ggaccaagat cctgcttccc aaggacggct gaggccgtca gaatcctttc ggagtagagc
     2461 cgcgggggcg gccgcgcgac gcgcggcagc gatcgagtcg cggtcccccc gcgctgctct
     2521 ccggtggctt cgtctcgccg cgcggctggc ccccgaattc gggctccctc accgccgcct
     2581 cttcgagctc tcccgcagtg tcggcgaccg catgggcgcc taccgcgcct cccgtaacgc
     2641 catcgtcaag tttccgcact ccgccgacgc ctggatgctg gccggccagg gagcggagct
     2701 cgtcttccgg caggcggagg cattgacggc ctacgagcag gcgatggtga tcgaggagcg
     2761 ggccgatgca gccctcgccg cggcggcgct ctatgtacgg gccggcaggc acgccgacgc
     2821 agcggcgcgt tttgcccggg gctttgcggc gggaggagga ccggaggcac tgcggcagaa
     2881 cgcggaatca ctggctcggg cgggagacac cgccgccgcc gaggaagcga tgcggctgtt
     2941 cagggagctt tcgaaccgcg ccggaacaag atgaacgagt agcccaggag gatcgccgcg
     3001 gcgatgatgt acgcggcgat cgcgtagccg cccgtgagtg gtgtgccgtc agcccacatg
     3061 cgaatcccct ctccctagtg cgtaacgctg cgtcacgaat ccgacgtaga gaatcgtgaa
     3121 cgtgaggaag gaaaagagca gcgtcgctag catgtcgccc gggagggagg gcgcgctcgg
     3181 cttgaggacg atcggctgcg ggtgcaaggt ccggaacagg taaacggtga gatgaatgaa
     3241 cgggaccagg gccgctccga agatccccag caccgccgag taccgggccc gggtgcccgc
     3301 ttcggggatg gccccccgca ggagcagata cccgataaag aggaagaaga gaaacaacgt
     3361 actcgtgagc cgggcatccc aggtccacca ggtgccccag atcggcttcg cccagagcgg
     3421 tcccgtggtc agcatcacgc acgcgaacac cgtccccacc tcggccgacg cggccgcgaa
     3481 acggtcgtac cgttcgtctt tcatcaacaa gtagaggatg ctcgccaagc ctaccaggct
     3541 gaacgcgagc atcgtcgacc acgcggcggg aacgtggacg tagaagatct tctgtgctcc
     3601 cccctgcaaa cgctccaccg gcgtgaacca gatggcgcgc acgtaaaccc ccgccaggag
     3661 cacgagcccc agcagcgtga tcgcacggcc tctggtcttg agggtcattc gtccaccgtg
     3721 gtgggaaaaa gtagcgtcgc gagcgacacg aacacgagat cgtacgcgag aagcaaccta
     3781 agccagccgg agacttcgct caagggacgg cccgagaaaa gccgcgccgt cacctgcacc
     3841 gcgcctacca gcggcgggat gaagaatggc agcaacagga ccggcagcat cagctcggcg
     3901 aaccggacgc gaatcaccaa cgccgacagg agggtcccga cggccacgaa gccgatcgtc
     3961 gccacggcga tcagcgccgc gagcggcagg agcacgtgaa cgatggagat gttgaagaag
     4021 agcgcgaaga gcggcagccc caccgcctcc acgaacccca cgaaacacag attggcgaag
     4081 agcttcccca ggtagagggc gcgccggtcg accggcgaca ccagcaagcc gtcgagcgcc
     4141 tggttctcga gctcgagctg gaaggcgcgg ttgaggctga gcaccgccgc gaacgtgaag
     4201 gtcacccaca ggatgctcgg tgccagatcc agcgcggcca cggccgtcgg gtcgcgaccg
     4261 aagttgaaca ccgtcaggac caatgccgtg aacaccagcg cggacgcgag gcccgtcttg
     4321 gtcctgagct cgagggccag atccttccgg gccagtaccc aggcgtcgcg caggatctca
     4381 cccatcagga caccagctcg cggtagcgcg cgccgatctg ttcggcggtc cgggcgttcc
     4441 ggggcatgaa ttcgacgagc cggccggtct tcagcacggc gacgtccgtg gcgaggtcgg
     4501 tcccctcctc cacgttgtga gtcaccagaa tggttgcccg tccctccccg gcacccgcga
     4561 attccttcac cagagcggtc agctccgacg cggcaacacg gtccaggccg gtgaaaggct
     4621 cgtccaagag cagcaccgac ggatcatgga tcagggcgcg cgcgatggcg gcacgctggg
     4681 tctgcccccg ggacagggtc ccgacgcggc ggtcgagctg ctcccgcaga tcgacccgcg
     4741 ccgtcagact cgcgatccgg gcatccagca cgggcgtgcg aacccgatag agcgatcccc
     4801 agaaccggag gttctcccgc accgtgagct ggccgtagag gagcgactga tgcccgatcc
     4861 agccgatcgc gccgcggcca ccctgtaccc cggcgtgccc gaggttcggc ttgatcaacc
     4921 ccgcaagaac tttcagaaga gtcgtctttc cggatccgtt cggtccgaaa agcgcgaggg
     4981 tgtgtccggc tggcagggtg aaggagacgt cgtcgagcgc gggaaccccg ccaaaacgat
     5041 ggcggacgcc gtcagcccac agcatcgatg cgggcagcga gctcgttgag atcgaagggt
     5101 ttcaccaggt agcccgagtg ccccaggtcc aggaccttca tcaggggctc catgctcacg
     5161 tacgccgtgg tgaccacgac cggcagggtg gcgtgcgtcg cgcggatctt ttgcagcagg
     5221 gcgataccgt cggtgtccgg aaggcggagg tccaggatca gcacctgggg gtcgaacgac
     5281 tccagctgcc gcagcgcctc ggcgcccgtg cgcgcgaacg ccatttcgta ccgcgcctcg
     5341 aagaagcgtg cgtacgaaga acggagcgcc gcttcatcct cgacgatgag gactcggcgg
     5401 cggggaggag cctcgaccgg catgggcgac aatatacctc aaccgcccga gcctgctccg
     5461 ccttccaatt caaagagatc gtccagcttg gtcagcacca gcaggaaccg gagctcttcg
     5521 ttcgcgcccc gcagggctac ggccacccgt cgctccgccg ctcgccgctg gatcagcatg
     5581 agcgtcccga gtccggccga gtccaccgcg cgggtagccg agaggtcgat caccagccgc
     5641 ccggcaccct ccggaagctg atcgagcagc tccacggcgg tacgccggaa cgccgtgcgg
     5701 ctctcgagag agagcgcctc gggtgcggcc aatgtctttt cggacgagcg cgaggacatc
     5761 gtcataagct cctgtaaaaa acctgggctc cgcgtcatgc cccccaataa gcgatatcgg
     5821 tgccaaatgc tgcaaaatca actaatacaa catgttagct cgccatcgtg actccgctca
     5881 cgccggttta ccaccgcaac ccgagggcgg tggcgcaaag tgcaaggcgg gaggtacgtt
     5941 cgaccacatg accgaccgca ttgagacact cctcgacgaa cagcgcacgt tcccgccgcc
     6001 ggccggattc acggcccagg cccatgtccg ggacgcctcg atctatcaga aggccaaggc
     6061 cgatcgtgag ggttattggg ccgattgggc ccggcagctc gaatggatga agccctggga
     6121 tcgagtgctc gaatggaccc cccctcacgc caagtggttc ctgggcggga agctcaacgc
     6181 cagcgtcaac tgcctggacc ggcacgtcaa agccggcaag agggacaaga tcgggttcat
     6241 ctgggaaggt gaacccggcg aagtacgcaa catcacctat ggcgacctcc tcgaggacgt
     6301 ctgccgttgt gcaaacggcc tcaagtcctt gggggtgaga aaaggggacc gcgtcgccat
     6361 ctacatgccg atggtgcccg aagtcgcggt ggccatgctt gcctgcgcgc gcctgggcgc
     6421 cgttcacacc gtggtattcg gcggcttctc gtctgagtca ctccgcgacc gcatcaacga
     6481 cgctcaggcc aaggtcctga tcaccgccga tggcggatac cgccgaggcg gccaggtccc
     6541 cctcaagcag aacgcggatg acgcactcac ggacaccccg tccattcagc acgtcgtggt
     6601 tctgaagcgc acgggacagg ccgtgagcat gaaggcgggg cgtgatcaat ggtgggacgc
     6661 gctcgtcacg ccgcagtcac cagagtgcgc ccccgagccg atggacgccg aggatcccct
     6721 gtacatcctg tacacgtcgg ggaccaccgg gaaaccgaaa ggcatcctcc acaccaccgg
     6781 cggctatctg acccaggtct tcgccaccac caagtgggtc ttcgatctca aggactccga
     6841 cgtattctgg tgcacggcgg acgtggggtg ggtgacgggg cattcgtacg tggtctacgg
     6901 ccccctcgcc gtcggtgtga cacaattgat gtatgagggc gcacccgacc acccgtccaa
     6961 ggaccgattc tgggccctct gtgccaagca tggcgtcacc gtgttctaca cggcgccgac
     7021 cgccatccgc tcgttcatga agtggggcac cgagctgccc gccaggcacg acctctcgaa
     7081 aatccgcctc ctcggctccg tgggcgagcc gatcaacccc gaggcgtgga tctggtacca
     7141 cgaaaacatc ggcggcaagc gctgtccgat cgtcgacacg tggtggcaga cggagacggg
     7201 cgggatcatg atcactcccc tgccgggcat cacgacgacg aaacccggct ccggtaccgt
     7261 cgcctttccc ggcatcgacg cggtgttgct ggatggagac ggcaaggaag tgaaggtcgg
     7321 tggggggtac ctggcgatca ccagcccgtg gcccggcatg ctgcggacca tctgggggga
     7381 tgacgatcgc taccggaacg tctactggag ccggtggccg aagacctact tccccggaga
     7441 cggcgccaag cgcgatgacg acgggtactt ctggattctg ggccgcgtcg acgacgtgct
     7501 caacgtcgcg ggccatcgca tcggtacgat ggaggtcgag agtgccctgg tggaccaccc
     7561 cgccgtcgcc gaagcggccg tggtgggacg cacccacgat ctcaagggcc aggcgctggc
     7621 cgcgttcgtg accatcaagg aaggccggca ggccaccgat gcgctccggg aagagctgaa
     7681 agggcacgtc gccaagaaga tcggcgcgct cgcccgccct gacgacatca tctatgccgc
     7741 ggatctcccg aagacccgaa gcggaaaaat catgcggcgc ctgttgaagg acatcgccga
     7801 gggacgcgcg ttgggagaca cgacgacgct ggccgatccc gccgtggtgc agaggctgaa
     7861 gacgctctac gaagacaagg agtgacgggg aacgggaaac gggaagcggg aaacgtgact
     7921 acttccgaag atggtgccgc acggtggtca ccacaacgtt cttccgaaag agcagcgcag
     7981 tcttgatcgc gtagacggtc tggttgtgca ggaaggcgtg ccaccagcgc gccggtacca
     8041 cttccggcaa cacgaccgtc agccaatccg gcttctccgt ccgctccacc tgatcgatga
     8101 actggaggag cgggctgaca atggcccggt agggcgacac caccacttcg agcgggaccc
     8161 cggaatccca cagatcccac tcgtcctgca tccgctggac ctgcttcggg tcggtcgcca
     8221 cggcgacggc gcggatgtgc ggcgacagca gcagggcgta cgcgagggcg ttggcggtgc
     8281 tcttggacac ccccgccacc ggcacgacca ccagatgttt gtcgggccat tccggttgcc
     8341 aggcgtcgct ggagagctgg cgcgcgtaga actggtagtg gcgcttgatg gcgaggaaga
     8401 cgaagaccag cgcgacgatc aacagggcgg agatccaggc cccttcggtg aacttggtga
     8461 cgagctcgat cacgacgacg acggcggtgg ccagcgtgcc gagcgatgaa gcgaccagcc
     8521 ggcccacccg ccgttctccc gccttgaggg atcgccgcac catccccgcc tgcgacagcg
     8581 taaacgagat gaacaccccc acggcataga ggtggatcag ggcgttggtg ttgccgccga
     8641 acacctcgag caggacgccc gccacgaggg cgagcatcag gatcccgttc gagaacacga
     8701 gccggtcacc ccggctcgcc agctgacggg gcaggtagcc gtcggtcgcc aggatcgacg
     8761 agaggcgggg gaaatcggcg aacgcggtgt tggccgcgag caccaggatc agcgcggtgg
     8821 agaactgcac ggcgtaatag aacgggccgt ttccgaaagc cccgtgcgca agctgcgaga
     8881 tcacggtctg atcaccgacc gggaccacgc cgaagcggtg ggcgatgatg gtcgagcccg
     8941 cgaagaacac gccgaggatc gcacccatcc agatcatcac ggtggcggca ttcctggact
     9001 cgggcgggcg gaacgcctgg ataccgttcg aaatcgcctc cactcccgtc agcgccgagc
     9061 acccggacgc gaaggccttg agcaggagcc agagcgtcac cgccgtcccg gtgctcaccg
     9121 gcaaggtcgc cggggtgaac gttccgaacg ggtgaaccac gtccacgatg agcatcaacg
     9181 cgaacatgcc gatgaaaaag taggtcggaa cggagaagat catccccgat tcccgcaggc
     9241 cccggagatt gccgatcgcg acgagcgcga tcgcaccgat gccgagggcc accgcgtgcg
     9301 gcgcggccgc cggaaatgcc gacgtgatcg cggcgactcc ggccgcgacc gacaccgtca
     9361 ccgtcagggt gtagtcgacg aggagcgccg cggcggccac cagtccgaac ccggtgccga
     9421 ggttttcctt ggcgacgaaa taggcgccac cgccctgcgg gtacgcaaac accgtctggc
     9481 gatagctgac cacgacgatg gtgagcagca ggagaatgaa gcccgccacc acgagcccgc
     9541 gtccgacgcc ggccgcgccg gcggtcatga ggacgatgag catcgcttcg gccgcgtacg
     9601 ccaccgacga cagcgcgtcg gaggagagca cggccagtcc ggtcgccttc gacagccgct
     9661 ccgactcgag gtgcagagtt tcgagggggc gaccgaccag cacgcgcttg gcgctgcgcc
     9721 agagggtcat gggttgtcgc tcaggacttt cggagggagc ccgccaggga ggtcacgaag
     9781 atggcggtga tcaccgcggc ggatgccaga caccagcggc accaggcgtg aatgacgaac
     9841 gcttcgaggg acgtcagata catggtgaag agaaaaccgg cgcccgacag caccagcagc
     9901 aaccgggtgg gccaggcgcg ggagaccatc gccggctgcg agccgacgat gctgatgccg
     9961 aagagggtga cgtacccgac gacgccgtag aacgcgacgg gaatgccgaa gagacgggcg
    10021 taggaggacg cctggacgat ctcacagccc ccggtgccac atttcagcgc gccgacgacc
    10081 ccgatggcgt gcagccacag gtacgtcgcg atcgcgatcc cgaccaacgc caggaccgcg
    10141 accgcttggc ggtgcaggct catttctttt tcttggggtg tgcggcgatg atactgtccg
    10201 cgagcctctg gaattcgtcc gacgaccggt cgcccctcgc gacttccttc ccgtcgatga
    10261 agaaggtcgg cgtgccactg acgcccagcg cgtcgccccg gtccagcgac gcctggatcc
    10321 ggccggcata gcggtcggcg gaaacgcacg cgtcccaggt cgtcgtgttg accccgaccc
    10381 cttgagccag cccacggaat acggaggtcg ggttgctgct ctgcagggcc cagtcgtggt
    10441 ggttgaacac cgcatcgatc atgtcccacg ccttcccctg ctccgaggcg catgccaccg
    10501 cgttcgcggc caggtgggac cagcggtgcg tcgtgggggg cagcggatag tcccgcagcg
    10561 tccactgcac cttgccggtc ttgatgagct ggtcacggat ggtgggaaac tgcaccgtgg
    10621 cgaacatcgc gcagatggga cactcgtagt cggcgaactc cacgactttc acgggggccg
    10681 aatcggagcc gaggacatag ccggggaagg cgctgtcctg ggcggttacc ggcgcccggg
    10741 acccggccga tccggagacg gcggacgccg gctgattcgc cgtgtagatg aggaacgcga
    10801 tagccgctac cgccacgacc gcgaggagcc cgtagaaccg cttcattctc cgcccgacct
    10861 ccgccagggt tgatcgtcct cgtcgtccgt ggcatcgaac cggagctccg aaaaccgccg
    10921 ctccgcttcc tccacgatac gccggctctc ggccacctcc ggggtgtcgt aggtggaccg
    10981 gacttcgtaa tgaaacagct cactgcgggc atccccgagg agctcctgca gctccggggc
    11041 cgactccagg aggcgccccg tgcggtccag ctccgcgatc cgcgccgcca gggcccgcag
    11101 gagcgcggcc agccgctccg cccgctcagg gccgtaagtc tcgtcgtggt cagacatcca
    11161 agggattata cactcccgga agtcaggacg gggttcaggt ttgcttcgcc ctgccccctt
    11221 cattagattg ccgccgctgt accgacagcc ccgacccaga gaggagcggc cccgaatggc
    11281 caaggcacgt accgacaccc gaaaagcccc gccgaccccg acgagctttg agcaggctcg
    11341 tgatgaactg ttctcccaca tcctccgctg cggcgttctc gaagccactt ccgagcatca
    11401 aaaggagtgg ttcgacgaca ccatgctgta tctcggtgac cgctacgagg atctgagcga
    11461 ggaagagatg acacagctgc gcgtcctggg cgagcggtat tgcaagcccg tgatggcccg
    11521 cgcggcaccc gcggtcgcca gcacctgatt cacggttccg ggagtctcgg ggcgcccctt
    11581 cgagggcgcc cttgtcgttt cccgggctag cgccaccacg cgggaaagcg ccgccaccgc
    11641 acccccaccg ccagtcgcgc acccccggac gcaccacctt ccacgtacca gccgaagcga
    11701 ttcccttccg cggcttcgac cccaaccgtc agcgtcaggg cgccgaacga atggcgcgag
    11761 tcgaccatca gacaattcac gcccatcccg acgtatgggg acacaccgct cttcgcggcg
    11821 gggagcacga ggaattgcgc ctctcccccc acccgtcccg cgggccgccc gtcgcgaacg
    11881 ccggccccgg ccgtcatggt gaaccgcacc tgccccgccg gccggagccc gagccccgcg
    11941 tcgagcccgt agtacggacg gcggccccag agcgcggcgg cgccgaggct cgtttcgctg
    12001 gccggtggtc cctgcgctgt ggcgacggcg gccccgcaga cgctgagggc cgcgagctta
    12061 ctccaccaca atgtcgtagg tcaccgggat gtcgggattg agggacgtga tcacggagca
    12121 gtacttctcg atcgaaaggt cgagcgccgt gcgcaccttg ctttcgtccg cgccggcgcc
    12181 cttcacccgc acgtgcagca ccatcgcggt gacgcgtcgt ggctcggtct cgcgccgcgt
    12241 gccggtgagc cgaatctcca gcttggtcag gtcgacccgc atcttcttca ggatgatgac
    12301 gacgtcggac gcggtgcacg acgccgcggc gaccagcaac gcttccaccg gtgacgtcgc
    12361 gaccttggtg tccccatcga ccagcgtcgg cttccccgac tgtcccgtgc cttcgaagac
    12421 cagaccctcg ccgagccagc gaagaccgat ggtcgcttga ggcgcggcca ctcagccccc
    12481 ccggcgggtg gtgagtccga tgtagaaccg gtggcggcgc agcgcgtcca tcccgtggta
    12541 ctcgaggagc cagttgaacg acgagcggta atggagctcg aaaccgagca ccaggttcac
    12601 ccagacggcg gggcagctgg gatcggcgcc gcacgcctga tggccggcga gcgatacgcc
    12661 ggcgccgacg tacgggatcg ccgtctggtt gtcgtccgtg aaccgccaga gggcttccag
    12721 gcttccgaca tacgtgttgc ccccgttgaa cacaccgatc tccgcggagg gacggatacg
    12781 tacctgcgga gtgaagaggt cgccgacgtc gagggtggtc ccgaggacca tctgcccgcc
    12841 gccgtgactc acgtcgatgc cgccccggac gccgaacccg aagagcccga ggcgggtgcc
    12901 ggtggacggc gtgctcgcgc cctggggggg agtgaactgc tgcgcgaatc cggcgccggg
    12961 cagaacactc aggacgcctg ccgtgatgat gaccttcagt cgactcacgt atcctccgtg
    13021 cgcgggcgca gggcccgacc gtagtcccac aacgcgtaca ccgaaatggc cgcggtggcc
    13081 catgcgagag gacgcaccaa aggggactcc acgacgaacg ccaccaacgt gaggatctgt
    13141 cccaccgtca ccgctttccc gcccgcccgc gccgggaacg gcactggacg atggaagagc
    13201 cagaccgcca atccccctcc ggcggcgaag atgtcgcgga gcagcacacc ggcgatctcc
    13261 caccagacca gcggtccgct ccgccagagc gtcaggaacg cggccgccat gaagaccttg
    13321 tccgccaccg gatcgagaat cgcgccggcc cgggagccgc cgtaccgccg ggcgacccgt
    13381 ccgtcgaaga aatccgatgc cgccgccacg ccgatgatcg ccaaccgcag cggcatcccc
    13441 gtcaccagcg ggaaggccac cgccagcgga agacgcagcg acgtcagcgc atccgcaggg
    13501 gtgaaccatg gtttcacttg ccggtccata cgcccccggg taccttgacc ctgtggacct
    13561 caatctgctg aaacaggcgg ccatcttcca agacctcgac gagggtgagc tcgcgcgggt
    13621 gtcggaagtg tgccgggaac agaagttcac cgtgggccag tacatcttca aggaaggcga
    13681 gcccgggaac cggctgttca tcattgccga aggggaagtg cgcatttcgc ggaccatccc
    13741 gggctccggc gaggaagccc tggccgtttt gaaaccgggg gcgaacttcg gtgaaatggc
    13801 gattttcgac cggtccgaac ggtccaccga cgccatcgcc aacatctcgt gcactctgat
    13861 caccatttct cgctccgatt tcgagctcct gctcgacttc aaccgtgaca tcgcgtacaa
    13921 ggttctctgg tcggtggtgc gcctcctctg cggccgcctg cgggtgacga acgacaatct
    13981 ccgttcgttc ctcgcgatgt cgatgttcta gggggtctcc caattcgtcc cgtccgtcct
    14041 gcactcatcg ccgccgcggc catcgccacg tccgccctgg cctgctcgca ctccgtctcc
    14101 accgcgccgc tgccgttcat caccgtccag cccctggtcg actccctgta cgtgggtgac
    14161 tcgggtgccg cgctgaccgc gacctactac aacggcaacg ggagcgtcgg tacttcgggt
    14221 ccgaagaact ggtccagcag cgacagcacc gtcgtcctgg tagacgcttc cagcggcgcg
    14281 ctcaaggcgg tcggcagcgg cacggccgtc ctgaccgtga ctgcgagcgg cgtgacggga
    14341 caggcgctcg tggtcgtgac cgacacgctc gatctggcat tgctgctccc ccagatcgtc
    14401 ctgatgcctc aagacacctt cgttgtcccg gtctccatcc ggaacaagag tggcggagcc
    14461 attgcgcccc catggttctc cgcctcggtc aacgggtact tcacggtcga ctcggccacc
    14521 gggcgcgtgg tgtcgacggg aaccggtgcc cccgccccgt atctcgccca cctcggcgcg
    14581 ctcacggcca acgggacggt gcaggtcgtc tcgatggctg acacgacggg cggcggggcg
    14641 gcatacaccg tcaacggcag cgtgagcgca cagcgctcgg ccagcacggt ccgggcgcag
    14701 aactacaccc gcgccggcgg aacacccacg ttcctcctga ccctcaaggt cgtggtgaac
    14761 ggcgtggcga ctgagctcgt taacgtcttg agccagaccg cggtaaacgg gggtcccgac
    14821 agcctgggcc tcgactcggt ctccgtcacg gaggcggaga gcaactcgtt cctctgctcc
    14881 ccgccgcgct cagccgccgc gtggacgtcg accgtgggcg ggaatccgat cctggccgtt
    14941 tcccggccgg ggggctatgt gaagatccgg caggagacta ccgtgacggg cgggcacgcg
    15001 atcgccggat cgttctactt caacgggcag cgcgcggacg actacacgga cccatcgggc
    15061 gtactcgcga tccggggcga tttcgtcgcc cccctgatca cggtcacgaa tacctgcaaa
    15121 tgaggatgcc atgaaccggt tgacaccgta cgcggcggtg ctgctgcgga tcgcggtggg
    15181 gctgatcttc ttgcatcacg ggattcagaa gctgcacatg ggcgctgccg gcgtcgccgg
    15241 attcttccac ggcgcgggca ttccgttctc gaccgtgttc gcctacatcg tgatcgcgct
    15301 ggagaccgtc ggcgccgtcc tcctcatcct cggtgtcatt ccgcgcatca tcgccatcgg
    15361 ctacgtgatc gagatgctgg tcgcgatctt cgtcgtcatt ctgccgagcc acccccagct
    15421 ccccgagctc gaggccctgc tctgcgccgg
//
LOCUS       DASUBP010000140         9602 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_998513, whole genome shotgun sequence.
ACCESSION   DASUBP010000140 DASUBP010000000
VERSION     DASUBP010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9602)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9602)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9602
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_998513"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2028
                     /locus_tag="VFU75_05210"
     CDS             <1..2028
                     /locus_tag="VFU75_05210"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transferrin receptor-like dimerization
                     domain-containing protein"
                     /protein_id="HEU5466266.1"
                     /translation="VPNPDSVRVNIRLLSAFPHEAGTERSRRVAETILARFKSFGLDA
                     HIEQFEALMPRPVSRTLELVGPERYTAVLKEPALPEDSTSGQTDQLPTFNAYSADGDV
                     TADLVYVNYGVPDDYKVLDSLGISVKGKIVIARYGGSWRGIKVKVAAEHGAVGCLIYS
                     DPRDDGYFDQDVYPKGPMRPDMGVQRGSVMDMPLYPGDPLSPGWGSVPGDTHRLSIAQ
                     ATTIETIPVLPISYGDALPLLRNLTGPVAPENWRGALPITYHIGSGPARVHLAVSFEW
                     KTRPLYDVIATIRGATDPDQWVVYGNHHDAWVNGAEDPISGQVALEETARALGTLVKS
                     GWKPARTIIFAAWDGEEWGLLGSTEWAERHAAELEQKAVVYYNSDTNDKGWLGAEGSH
                     SLEAFMEQVARDVRDPATGKSVLTALLDHERTERHADSTAGNAFHLGALGSGSDYSVF
                     IDHLAVPSVSTGFGGATPSGIYHSIYDSYSFYDRFLDSGFAYGVTEAQTMATAVLRMA
                     DAPLLPYEFTRAASTYRKYADEIAKAAKKDSQTRSLDLSAVQAALVRLDSAAAEYGRV
                     SARLDSMPSTTLVKRRPALLAINQSLARAERALSDTTGLPRRPWYRHLLYAPGFYTGY
                     GVKTMPGIREGVDQGKAAEAQAQAARVAAALDRYTAVIHQAAQALQPVLAH"
     gene            2087..2716
                     /locus_tag="VFU75_05215"
     CDS             2087..2716
                     /locus_tag="VFU75_05215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466267.1"
                     /translation="MSNPMPPRPSVGRSSFAIAMKDYTFIRPGIAVTISAATLLIAFI
                     FGIDLIHSLNRASTTVRTGAQVSQTLHSYSAAYEVWHQMATSTDAAYKTPQAQSQRDQ
                     LRDHLRTNLADLGKTLADTSDQAQVRSILEGLASVDPAATMNARQSMTILLGRQDAAL
                     FDAAETSQQGVLLAALLLGLTVIAAATLVVPMAWLYIRYKRGATIEVKL"
     gene            2829..4508
                     /locus_tag="VFU75_05220"
     CDS             2829..4508
                     /locus_tag="VFU75_05220"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5466268.1"
                     /translation="MAFFSHTAPGSGRPRGVLVAVLLVATLGITALLAYQSVLEVTSH
                     RATAERVLRDYSAFAAHQLSTRTSNELGYYGFGRALGALDRLRTETHQNALPSPSELG
                     QRSDSATRAFLSKMSFAFDLTFPKHDLVIRGAPADSALRRWVTDTLDSYVRGVHRPEW
                     ADAVIVGAPSAHRLMLAYKLERDAKGAPREAVGFAAELSTLTPLFIDAAESGPLIPPT
                     LTGGIPYDSIGSVAIQDADGRDIYRSPGGFPALFTGHDTSTTMLGHLRFEVTLRPDMG
                     PKLVIGGLPRSRLPLITGLLFITAILVAGILYQLRREYALAALRSDFVASVSHELRTP
                     LAQIRMFSETLRLGRVRSQEERTRSLVIIDQEARRLTHLVENLLHFSRSERYAARIDA
                     APEHIAPLIRSAVEKFEPLAAARRVTVRTVLDERLVAVVDPGALHQMLLNLLDNAVKY
                     GPDGQTVTITLEGWGGYLRIKVDDLGPGVPVEARERVWDRFWRLRRDRGSSVAGTGIG
                     LAVVKELAVLHRGRVWCDEAEAGGARFVIELPDATPPQPLTQPQQAMEALT"
     gene            4505..5191
                     /locus_tag="VFU75_05225"
     CDS             4505..5191
                     /locus_tag="VFU75_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5466269.1"
                     /translation="MSKVLVIEDNQDLAYGLRNNLEIEGHQVDVAGDGAEGLARVRKT
                     EPDLVILDLMLPGLDGFRVLRTLRDEGRRLPVLILTARGDESDKVRGLRLGADDYVTK
                     PFGVLELLARVEALLRRATAAEPPKAPPVETFGDIEVHTASRTVRRHGEAVTLTPKEY
                     DLLLALIDRRGAVASRAELLTEVWGYSAAVMSRTVDTHIAELRRKLENDLAVPRHILT
                     VRKTGYRLER"
     gene            5289..6032
                     /locus_tag="VFU75_05230"
     CDS             5289..6032
                     /locus_tag="VFU75_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466270.1"
                     /translation="MRFGILVVPLMLLGAGNPPAPRNGQELIRAMHDRYAGQWYKTLS
                     FTQNNTATDTAGKETHSVWKEYANIPGQLRIDFLPADSGQGYLFRADSLYVFGHDSLR
                     RAAPFLHPLMILGFDVYATPAETTIAKLTKLGFDLSKIHEDTWDGHPAYVVGVDKGDT
                     RHKQFWIDKDRLLFLRIVEPGQRDTTVLQDTRFEDYRAIGKAWLSARVRFLINGKPSW
                     LEEYIDIRTGDPLAATLWDPKQFGSARPK"
     gene            complement(6088..6780)
                     /locus_tag="VFU75_05235"
     CDS             complement(6088..6780)
                     /locus_tag="VFU75_05235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HEU5466271.1"
                     /translation="MLDLKLSTHRRGFLGRVAAGAAALGLGSVVSPVAAAAENAGVTP
                     AADPAFEGWLNKINGKHKSMYDAPEVNSGFCFIWARVWLNTCNATYGTTDADNTAAIV
                     VRHSAFALAVQDSAWAKYKLGEFIKLNDAKTGAPSVRNPFYKMAAADQLLPGYGIDEL
                     LSKGVLVGVCNVALTVFSGMLASKLGGGLTGDGVKNDLVANLIPGVVVVPSGVLAVNR
                     TQEKGCTYTFAG"
     gene            complement(6822..7646)
                     /locus_tag="VFU75_05240"
     CDS             complement(6822..7646)
                     /locus_tag="VFU75_05240"
                     /inference="COORDINATES: protein motif:HMM:NF044772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HEU5466272.1"
                     /translation="MKFSWSILLLLAACRGTATPAVSSVRVPADSEIPSGPLGASIRR
                     GLALLTATHDSLPTYVGNGLRCVSCHLDEGRRSPQSWVGVTGRYPQYRSRSGTVETIE
                     YRINGCFLRSLNGKAIPVDGNDMRDIVSYLTFLSTGQRVGYKAPPPDTRWSGLRPDTA
                     AGNKFFATNCARCHGEDGGGTIAAPPVWGPRSYNIGAGMSRARTAAPFIVANMPFDKA
                     ITLTDQQALDVAAYINSRPRPDFAGKANDWPNGDAPPDVAYQTKAAHPAPPGSRAH"
     gene            7707..8462
                     /locus_tag="VFU75_05245"
     CDS             7707..8462
                     /locus_tag="VFU75_05245"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU5466273.1"
                     /translation="MTPWIKRLILANIVVYILETQSPALINYLQLSRISLVVAPWTLI
                     TYMFTHAGFQHILWNMLGLFFLGPRVEVRLGGPRFLGLYFIGGLAGGAMSLALSPAPI
                     VGASAAIFAVYLAYAMFWPRDRLLIWGLIPVPAIVLVGGLTAISILGGGGYIEPGVAH
                     WAHLGGFVGGYLYLTFLKHQTGSQKFRAKATPAAPLPKSPETVARWRSIDPAGLHPVN
                     REELGRIMTKLDQGGPASLSSDERSFLERFANN"
     gene            complement(8463..>9602)
                     /locus_tag="VFU75_05250"
     CDS             complement(8463..>9602)
                     /locus_tag="VFU75_05250"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEU5466274.1"
                     /translation="DPDGAGRSPLLIVGGSADQRGVVTSASYEARAYGVSSAMPMARA
                     ARLCPDAMIVPVPWEACVEKGEEIRAVLERFTPTVERASSDEFYLDMSGTERLYKNEH
                     LSETARRIRTAVKDTTKLSVSIGGGTSKLVAKLGAGRAKPRAGVEGKAVVIVEPGAEE
                     AFLREFSLADLPGVGPRFQEELARFGLREVTDALRHDLKTLQGWLGERAGAWLHRRIR
                     GQDESQVDSSGDAKSISRDETFAKDLNDAESLDRELVALAGRASADLRASGLAARTVT
                     VRIKDADFTLRQSSRTLPAPIISDRAVLATARELLATLRKARHVPARLLGVGLSQLLR
                     EGAPDQLTLFESAPQGVETERDRTLSRMVDQIRDRYGPDAAGWGR"
BASE COUNT         1583 a         3330 c         3082 g         1607 t
ORIGIN      
        1 gtgcccaacc cggacagcgt gcgcgtcaac atcaggctcc tgtcggcctt cccgcacgaa
       61 gcggggaccg agcgctcccg gcgcgtggcc gaaacgatcc tggcccgctt caagtcgttc
      121 gggctcgacg cccacatcga gcaattcgaa gcgctcatgc cccgtcccgt ctcccgtacc
      181 ctcgagctgg tggggcccga gcggtacacg gcggtgctga aggagcccgc actccccgag
      241 gattcgacct ccgggcagac ggatcagctg ccgacgttca acgcgtattc ggcggacggt
      301 gacgtcaccg ccgacctggt ctacgtgaat tacggcgtgc ccgacgacta caaggtcctc
      361 gacagcctcg gcatcagcgt gaagggcaag atcgtcatcg cgcggtacgg tggctcgtgg
      421 cgcgggatca aggtgaaagt cgcggcggaa catggcgccg tggggtgcct gatctattcg
      481 gacccgcggg acgacggcta tttcgaccag gacgtctatc ccaagggacc gatgcggccc
      541 gacatgggcg tgcaacgggg cagcgtgatg gacatgccgc tctacccggg cgatccgctt
      601 tcacccggtt ggggctcggt gccgggcgac acccatcgtc tttccatcgc gcaagccacg
      661 accatcgaaa cgatccccgt tctgccgatc agctatggcg acgccctgcc cctgctgcgg
      721 aacctgactg gccccgtcgc cccggagaat tggcgtgggg cgctgccgat cacctatcac
      781 atcggctcgg gcccggcgcg cgtgcacctg gcggtgtcgt tcgagtggaa gacccgaccg
      841 ctctacgacg tgatcgcgac catccgtggg gcgaccgacc ccgaccagtg ggtcgtgtac
      901 ggcaaccacc acgacgcgtg ggtgaacggc gccgaagacc ccatctcggg ccaggtggcg
      961 ctcgaagaga ccgcccgcgc actcgggacg ctggtgaagt cgggatggaa gcccgcgcga
     1021 accatcatct tcgccgcgtg ggatggtgaa gagtggggac tcctcggctc cacggagtgg
     1081 gcggaacggc acgccgccga gctggaacag aaggccgtcg tctactacaa ctcggatacg
     1141 aatgacaaag ggtggctcgg cgccgaaggt tcgcacagcc tcgaagcctt catggagcaa
     1201 gtcgcgcgcg acgtgcgtga ccccgcgacc ggcaagagcg tgctcactgc tctgctcgac
     1261 catgagcgca cggaacgaca cgccgattcg acggcgggca acgccttcca cctcggcgcg
     1321 ctcggctccg gctccgacta ttctgttttc atcgatcacc tggcggtccc atcggtctcc
     1381 accggcttcg gcggcgcaac gccgtccggc atctaccact cgatctacga ctcgtactcg
     1441 ttctatgatc gattcctgga ctcaggattc gcttacggcg tgactgaagc gcagaccatg
     1501 gcgacggcgg tgcttcgcat ggcggacgcc ccactcctgc cgtacgagtt cacgagggcc
     1561 gcgagcacct accgcaagta cgccgacgag atcgcgaagg cggccaagaa ggactcgcag
     1621 accaggtccc tcgatctctc cgctgtgcag gcggcgctgg tccgcctcga ttccgccgcg
     1681 gccgaatacg gccgggtgtc cgcgcggctc gattcgatgc cgtcgaccac gctcgtgaaa
     1741 cgccgtccgg cgctcctcgc catcaatcag tcgctggccc gagcggaacg ggcactctcg
     1801 gacaccaccg gactgccccg tcgtccctgg taccggcatc tcctctacgc accggggttc
     1861 tataccgggt acggtgtgaa gacgatgccc ggaatcagag agggcgtgga tcaggggaag
     1921 gccgccgagg cgcaggcgca ggcggcccgg gtggcggcgg ccctggaccg gtacacggcc
     1981 gtgattcacc aggcggcaca agccctccag ccggtgctcg cccattgacg cctcttccgg
     2041 tgacggatac cgtaggggcc tcttctcaac gagttctcgg agcccgatga gcaatcccat
     2101 gccgccgcgc ccgtccgtcg gacgttcgtc cttcgccatc gcgatgaagg actacacgtt
     2161 catccggccc ggcatcgccg tcaccatcag cgcggctacg ctgctgatcg ccttcatttt
     2221 tggcatcgac ttgatccact cgctcaaccg ggcgtcgacc acggtgcgga cgggtgccca
     2281 ggtttcgcaa accctgcaca gctactcggc ggcctacgaa gtgtggcatc agatggccac
     2341 gagcacggac gcggcgtaca agacgcctca agcccagtcg cagcgcgatc agctccgcga
     2401 ccatctccgg acgaatctcg cggacctcgg caagaccctg gcggacacgt ccgaccaggc
     2461 gcaggtgcgg tcgattctcg agggtctcgc gagcgtcgac ccggcggcca ccatgaatgc
     2521 ccggcagtcg atgactatcc tcctcgggcg gcaagacgcg gcgctcttcg acgcggccga
     2581 gacctcccag caaggcgtct tgctcgcggc cctgctcctc ggtctcacgg tcatcgcggc
     2641 ggcgacgctg gtcgttccga tggcctggct gtacatccgc tacaagcgcg gcgcgaccat
     2701 cgaagtgaag ctgtaactcc ctgccacggc ggccccggcc gggacggcca ggaatggctg
     2761 gggcgccgag gttaggatcc cgtgaggctc gtgcggaccg gggtaccccc gagtagtttc
     2821 agggtccgat ggctttcttc tcccatacag cgccaggcag tggccggccc cgcggggtcc
     2881 tggtcgccgt gttgctggtg gccactctgg gcatcaccgc gctcctcgcg tatcagtcgg
     2941 tcctcgaggt cacgtcgcac cgcgccacgg ccgaacgcgt acttcgcgat tactcggcgt
     3001 ttgccgcaca tcagctgtcc acccgcacct ccaacgagct cggctactac gggttcggcc
     3061 gcgccttggg tgcgctggat cgcctgagga cggagactca ccagaacgcc ctgccgtctc
     3121 cgagtgagct ggggcaacgg tccgattccg ccacccgcgc gttcctttcc aagatgtcgt
     3181 tcgccttcga cctgaccttt ccgaaacatg atctcgtgat tcgcggcgca ccggcggact
     3241 ccgcgctgcg gcgatgggtc acggacacgc tcgacagcta cgtccggggc gtgcaccgtc
     3301 cggagtgggc cgacgccgtg atcgtgggag cgccgtccgc ccaccggttg atgctggcgt
     3361 acaagctgga gcgcgacgcc aagggagcgc cccgtgaagc ggtcggcttc gcggccgagc
     3421 tttcgacgct gactccactg ttcatcgacg ccgcggagag tggtcccctc atcccgccga
     3481 cgctgaccgg cggcatcccg tacgactcca tcggatcggt cgcgatccag gatgcggacg
     3541 ggcgggatat ctaccgctcc cccggcgggt tccccgccct cttcacgggg cacgatacct
     3601 cgacgaccat gctcggccat ttgcggtttg aagtgaccct gcgtcccgac atgggcccca
     3661 agctcgtcat cggcggactg ccccgctcgc ggctcccact catcaccgga ctgctcttta
     3721 tcacggccat cctcgtggcc ggcatcttgt atcagctgcg ccgggagtac gcgctcgcag
     3781 ccctgcgttc cgatttcgtc gcgagcgttt cccacgagct gcgcacgccg ctggcccaga
     3841 tccggatgtt ctcggagacc ctccggctcg ggcgggtgcg gtcgcaagag gagcgtacgc
     3901 gctctctcgt catcatcgat caggaagccc ggcgactgac gcacctggtg gaaaacctgc
     3961 tccacttctc gcggtcggag cgctacgcgg cccgcatcga cgcggcaccc gagcacatcg
     4021 cgccgctgat caggtccgcc gtggagaagt tcgagccact cgcggccgcc cgccgagtca
     4081 ccgtacggac cgtattggac gagcgcctgg tcgcggtcgt ggacccggga gcgttgcatc
     4141 agatgctcct caacctcctc gacaacgccg tgaaatacgg gccggatggc cagaccgtca
     4201 ccatcactct cgagggctgg ggtggctacc tccggatcaa ggtcgacgac ctcggtcctg
     4261 gtgtaccagt ggaagcccgc gagcgggtgt gggatcgctt ttggcgcctg cgccgggatc
     4321 gggggtcgtc cgtggccgga acgggaatcg gactcgcggt ggtgaaggag ctcgcggtgc
     4381 tccaccgtgg gcgggtgtgg tgcgatgaag ccgaagcggg cggggcgcgc ttcgtgatcg
     4441 agctccctga tgccacgccc ccacaaccac tgacgcaacc gcagcaagcc atggaggccc
     4501 tgacgtgagc aaagtgctgg tcatcgagga caatcaggac ctcgcctatg gtctccggaa
     4561 caacctggag atcgaaggtc atcaggtgga cgtcgccggg gacggcgcgg agggattggc
     4621 ccgggtccgg aagaccgagc ctgatctcgt catcctcgat ctgatgctgc cgggactcga
     4681 cgggtttcgg gtgctgcgaa cactgcggga cgaaggtcgc cgcctgccgg tcctgatcct
     4741 caccgcccgg ggggacgagt ccgacaaggt tcgggggctc aggctcggcg ccgacgacta
     4801 cgtcaccaag cccttcggtg tactggagct cctggcccgc gtcgaagccc tcttgcgccg
     4861 cgccacagcc gccgagcctc ccaaggctcc gccggtcgag acgttcggtg acatcgaggt
     4921 ccacaccgcc tcccgcacgg tccggcggca tggcgaggcg gtgacgctca cgcccaagga
     4981 gtacgacctc ctcctcgcgc tcatcgaccg gcgcggcgcc gtcgcgtcac gggcggagct
     5041 tctcaccgaa gtgtgggggt acagcgccgc cgtcatgtcc cggaccgtcg atacccatat
     5101 cgccgagctc cggcggaaac tcgagaacga tctcgccgtt ccgcgccata ttctcactgt
     5161 ccgaaagacc ggataccggc tcgagcgctg aacagcaggc gtcaggtacc aggggtcagg
     5221 cgtcagatgc tcccaccgcg cccgtgaccc ccgaccccga cccctgaccc cttgaacgga
     5281 ggatgcccgt gcgcttcgga attcttgtcg ttcccctgat gctcctcggc gccgggaatc
     5341 caccggcccc caggaacggg caggagctga tccgggccat gcacgatcgc tacgcgggtc
     5401 agtggtacaa gacgttgagc tttacgcaga acaacacggc caccgatacc gccggcaagg
     5461 agacccactc ggtctggaag gagtacgcga acattccggg ccagctgcgc atcgacttcc
     5521 tgcccgcgga ttcgggacag gggtacctgt tccgcgccga ctcactctac gtcttcggcc
     5581 acgactccct gcgccgggcc gctcccttcc tgcaccccct gatgatcctc gggttcgacg
     5641 tctacgcgac gcccgccgag accaccatcg cgaagctgac gaagctcggg ttcgacctgt
     5701 cgaagatcca cgaagacacg tgggatgggc atccggctta tgtggtcggc gtcgacaagg
     5761 gagacacccg acacaagcag ttctggatcg acaaggaccg cttgctgttc ctgagaatcg
     5821 tggagccggg acagcgggac acgacggtgc tgcaggacac gcgcttcgag gactatcgcg
     5881 ccatcgggaa ggcctggctc tcggcgcggg tgcggttcct gatcaacggg aagcccagct
     5941 ggctggaaga gtacatcgac atccgtaccg gagacccgct ggccgccacg ctgtgggacc
     6001 cgaagcagtt cgggagcgcg cggccgaaat agcgggacga gggaggaggg aggagtcgac
     6061 ccctcccccc tccctcctcc ctttgtctca tccagcgaac gtgtaggtac agcccttctc
     6121 ctgcgtccga ttgaccgcca gcactcccga cggcaccacc acgacccccg ggatgaggtt
     6181 ggcgaccaga tcgtttttca cgccgtcccc cgtcaatccg ccaccgagtt tcgacgccag
     6241 catgccgctg aataccgtga gcgcgacgtt gcagacgccg acgagaactc ccttggagag
     6301 cagctcgtcg atgccgtatc cgggcaggag ctggtcggcg gccgccatct tgtagaacgg
     6361 attccggacc gacggggcgc cggtcttggc gtcattgagc ttgatgaatt ccccgagctt
     6421 gtacttggcc caagccgaat cctggacggc gagagcgaag gcagagtgcc ggaccacgat
     6481 ggccgccgta ttgtccgcgt ccgtggtccc gtaggtggca ttgcaggtgt tgagccacac
     6541 ccgcgcccag atgaaacaga acccgctgtt gacctcgggc gcgtcgtaca tcgacttgtg
     6601 cttcccgttg atcttgttca gccagccctc gaacgccggg tcggcggcgg gagtcacgcc
     6661 cgcgttttcc gcggccgcgg ccaccggtga gacgacggac ccgaggccga gcgcggcggc
     6721 gccggctgcg acgcgtccca gaaagccacg tcggtgggtg gagagcttca ggtcgagcat
     6781 gttcgagacc tccaagtggt acgggtgaac gcgcccgaca gtcagtgggc gcgggatccg
     6841 ggcggcgccg ggtgcgccgc tttcgtctga taggcgacat cgggaggcgc gtcgccgttc
     6901 ggccaatcgt tggccttccc tgcgaaatcc gggcgtggcc gcgagttgat gtaggcggcg
     6961 acgtcgaggg cctgctggtc cgtcagggtg atcgccttgt cgaacggcat gttcgccacg
     7021 atgaacggcg ccgccgtccg ggcccgcgac atgcccgccc cgatgttgta cgacctggga
     7081 ccccagaccg gcggagccgc gatcgtgccc ccaccgtcct ccccgtgaca acgggcgcaa
     7141 ttcgtcgcga agaacttgtt gcccgccgcc gtatcaggcc tgaggccgct ccagcgggta
     7201 tccggagggg gggctttgta gcctacgcgc tggccggtcg acaggaacgt caagtacgat
     7261 acgatgtccc gcatgtcgtt gccgtcgacg ggaatcgctt tgccgttcag cgagcgaagg
     7321 aagcagccgt tgatccggta ttcgatggtt tccaccgtgc cggagcgcga gcggtactgc
     7381 gggtaccggc ccgtgacgcc gacccacgac tgtggcgagc ggcgtccctc gtcgaggtga
     7441 cagctgacac accgcaggcc attgcccacg taggtgggaa ggctgtcatg cgtggcggta
     7501 aggagcgcga ggccgcggcg gatcgacgca cccagaggac ccgacggaat ctcggagtcg
     7561 gccggaacac gcacggacga gaccgctggc gtggcggtcc cgcggcaggc ggcgagcagg
     7621 agcaggatcg accaggagaa cttcatgggg cgcggaaaaa ctaaccctcg ttgcggcgtc
     7681 ggcaacctcc atctagcgtt ctcgacatga cgccttggat caagcggctg attctggcca
     7741 acatcgtggt ctacatcctg gagacccagt ctccggcgct gatcaattac ctgcagctca
     7801 gccggatcag tctcgtggtg gcgccgtgga ccttgatcac gtacatgttc acgcacgccg
     7861 gattccagca catcctctgg aacatgctcg gattgttctt cctcgggccg cgcgtcgaag
     7921 tccgtcttgg cggtccgcgt tttctcggcc tgtacttcat cggaggactt gccgggggag
     7981 ccatgtccct ggcactcagt ccggctccga tcgtgggcgc ctcggcggcc atcttcgccg
     8041 tgtacctcgc ctacgccatg ttctggcccc gtgaccgcct gctcatctgg ggcctgatcc
     8101 cggttcccgc catcgtcctg gtcggcggcc tgacggccat ttcgatcctc ggcggaggcg
     8161 gctacatcga accaggggta gcgcactggg cgcacctggg cggattcgtg ggcggttatc
     8221 tctacctgac cttcctgaag catcagaccg gcagtcagaa attccgggcc aaggccacgc
     8281 cggccgcccc cctgcccaag tcacccgaaa ccgtcgcccg gtggcgcagt atcgatcccg
     8341 ccggactgca ccccgtcaac cgggaagagc tggggcgcat catgacgaag ctcgaccagg
     8401 gcggcccggc gtccctgtct tcagacgagc gcagcttcct cgaacgcttc gcgaacaact
     8461 agttaacggc cccaccccgc cgcatcggga ccataccggt cccggatctg gtctaccatg
     8521 cgtgagagcg tccggtcccg ctccgtctcg actccctgtg gcgcgctctc gaacagcgtg
     8581 agctgatcgg gagccccctc ccgcaagagc tgcgagagcc cgacccccag cagccgcgcc
     8641 ggtacgtgac gggctttccg caacgtcgcg agcagctcgc gcgccgtcgc cagtacggcg
     8701 cggtccgaaa tgatcggcgc cggcaacgtc cggctcgatt gccgcaaggt gaagtcggcg
     8761 tccttgatcc gcaccgtcac cgtcctcgcc gcgagccccg acgcccgcag gtccgccgac
     8821 gcccggcccg ccaatgccac gagctcccgg tccagtgact ccgcgtcgtt caggtccttc
     8881 gcgaaggttt cgtccctcga gatggacttg gcgtcaccgg agctgtcgac ctgcgactcg
     8941 tcctgcccgc gaatccgccg gtgcagccac gcacccgcgc gctcgccgag ccatccctgg
     9001 agtgtcttga ggtcgtggcg gagggcgtcg gtcacctcgc ggagcccgaa acgcgccagc
     9061 tcttcctgaa aacgcggacc tacgccgggg agatcggcca gagagaattc gcgaagaaaa
     9121 gcctcttccg ccccgggttc cacgatcacg accgccttcc cttcgacccc cgctctgggc
     9181 ttcgctctcc ccgctcccag cttcgctacg agcttcgacg tcccgcctcc gatcgacacc
     9241 gacaacttgg tggtgtcctt cacggcggta cggatgcgtc gcgcggtctc cgaaaggtgc
     9301 tcgttcttgt agaggcgctc cgtgccggac atgtcgaggt agaactcgtc cgagctcgcg
     9361 cgctcgaccg tgggcgtgaa cctctcgagc accgcgcgga tctcctcgcc cttctccaca
     9421 caggcttccc acggcaccgg gacgatcatc gcgtcgggac acaggcgcgc cgcccgcgcc
     9481 atcggcatgg cggaggagac cccgtacgcg cgtgcttcgt acgacgcgga cgtcaccacg
     9541 ccgcgctggt ccgcagatcc accgacgatg aggaggggag atcgtcccgc gccatccgga
     9601 tc
//
LOCUS       DASUBP010000141         3955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_153790, whole genome shotgun sequence.
ACCESSION   DASUBP010000141 DASUBP010000000
VERSION     DASUBP010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3955
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153790"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..561)
                     /locus_tag="VFU75_05255"
     CDS             complement(<1..561)
                     /locus_tag="VFU75_05255"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HEU5466275.1"
                     /translation="MRTTTQRLMLRGTVIVGFALAVSAVGGWVRRATADNVTLPEATV
                     PAADVRGMEQTLDQVQGELTVAKLQLERATAIIGFSSKYGIPADLASNIYDMSSSEGV
                     DPVLAFRLVRVESGFDPRAKSRAGAIGLTQVLPSTARLYEPGLSVEQLYEPATNLRLG
                     FRYLHDLMARYGTLNHALVAYNSGPGK"
     gene            complement(622..1086)
                     /gene="moaC"
                     /locus_tag="VFU75_05260"
     CDS             complement(622..1086)
                     /gene="moaC"
                     /locus_tag="VFU75_05260"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEU5466276.1"
                     /translation="MKLSHLDQEGRARMVDVSGKPATARSAVAEGTIRMSEEAFRLVR
                     DQAVAKGDVLGVAQVAGTMAAKRTAELIPLCHPLALDHIAVEATLDEALPGVQVRASA
                     RMVGRTGVEMEVLTAVSVALLTVYDMVKAVDRHMEIGPVRLVEKHGGTFIEA"
     gene            complement(1083..2135)
                     /locus_tag="VFU75_05265"
     CDS             complement(1083..2135)
                     /locus_tag="VFU75_05265"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466277.1"
                     /translation="MPLPPIAGHEALRARFADAIRAGRLPQTLLFEGPRGVGKQRLAL
                     WLAQTLLCETASAPCGKCASCRQVENLSHPDLHWFIPVELTKKGADADKQIDLVEEAI
                     GEVLAERREEPFYAAPAGLAAHSMASARLMLRRLALRPVQGSRAVFILGDAERLAAQK
                     GQDGAANAVLKALEEPPPTAFIILTTSEPDALLPTILSRTVRVRVGRLPDSVVTSFVQ
                     QHMMSRLGDAASVARRVQGAEGAIGRLVATEGNEGSGAAAADRLLQAIRGGMAARYGA
                     ALAQPPFQARGAFTSMLDALLERLRDEAREGGDTSKVMAAMTRVLEARELAQGNVNPQ
                     LLTAVLTEDLAGGGRS"
     gene            complement(2333..2890)
                     /locus_tag="VFU75_05270"
     CDS             complement(2333..2890)
                     /locus_tag="VFU75_05270"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5466278.1"
                     /translation="MQILIVEDESVTREVLKRLLLAAGHAVTDAADGAEAWEVLQSIR
                     IPVVISDWEMPQMEGLDLCRKIRSRSGEYTYFIMLTAKSGRAEYLEAMDGGVDDFLAK
                     PLDAVDLQCRLRVAERILGLRREVQQLEGLLPICSYCKRIRDEMNRWTSIETYIEQRS
                     RAEFSHGICPDCYEKHIEPVLNPGQ"
     gene            complement(2909..>3955)
                     /locus_tag="VFU75_05275"
     CDS             complement(2909..>3955)
                     /locus_tag="VFU75_05275"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5466279.1"
                     /translation="MQRDLPAGSRSREDVDEIQRSAARAAGLTRQLLAYSRRQVLQPE
                     VLDLNVVVSEMDKMLQRLIGEDVELVTILGEDLGRVMADPGQIEQVLLNLAVNARDAM
                     PQGGRLTIRTANRRDTPVPGTSDQPVSRVMLAITDTGIGMDDRVRSHLFEPFFTTKEV
                     GKGTGLGLATVYGIVRQSGGSISVESAPGKGSTFSVFLPRVEDASFRRASLPAAVPAD
                     SGEKRAPAAQILLAEDEPRVRLLTQRVLEARGYRVLSATDGQVALELASDPALTLDLL
                     VTDVVMPGMSGPDLARRLRESRPGLPVVYMSGYADQGLAHEAAATGGAFLSKPFETET
                     LERLVQSALDRRPQ"
BASE COUNT          658 a         1377 c         1208 g          712 t
ORIGIN      
        1 cttgccgggg ccgctgttgt aggcgaccag ggcgtggttc aacgtaccgt accgggccat
       61 cagatcgtgc agataccgga agccgaggcg caggttggtc gccggctcgt acagctgctc
      121 gacggacaac ccgggctcgt agagacgcgc ggtgctgggg agcacctgcg tcaggccgat
      181 cgcgcccgcg cggctcttgg cccgcggatc gaaacccgat tccacccgga ccagccggaa
      241 ggccagcacg gggtctacac cttccgagct cgacatgtcg tagatattgc tggccaggtc
      301 cgcgggaatc ccgtacttgc tggaaaaccc gatgatggcg gtcgcccgct ccaactgcag
      361 cttggccacc gtcagctcac cctggacctg atccagagtc tgttccatgc ctcgcacgtc
      421 cgcggctgga accgtggctt cggggagggt cacgttgtcc gcagtggcgc gacggaccca
      481 tccgccgacg gcactcaccg ccaacgcgaa ccctacgatc accgtccccc gcagcatcag
      541 tcgctgggtc gtggtgcgca tatacctctc ttgtgaagaa ctgtctccgg gacaacctgc
      601 catccccggt tcaaagctcc atcaagcttc gatgaaggtc ccgccatgct tctcaaccag
      661 gcgcaccggc ccgatttcca tatgccggtc caccgccttc accatgtcat agacggtcag
      721 gagcgcgacg ctcacggccg tcaggacttc catttccacc ccggtccggc ccaccatccg
      781 ggccgacgcc cggacctgta ctcccggcag ggcctcgtcc agcgtcgcct cgacagcgat
      841 gtggtccagc gccagcgggt ggcagagcgg gatcaactcg gccgtccgct tggccgccat
      901 ggtccccgcc acctgcgcga cgccgaggac gtcgcccttc gccaccgcct ggtcccggac
      961 cagccggaac gcttcctccg acatccggat ggtgccttcg gccaccgcgc tgcgggccgt
     1021 cgcgggcttg ccgctgacgt ccaccatgcg ggcacggcct tcctgatcca ggtgcgagag
     1081 cttcacgagc gccccccacc ggccagatcc tcagtcaaga cggccgtcag gagctgagga
     1141 ttcacgttgc cctgggccag ctcccgcgct tcgagcaccc gtgtcatcgc cgccatcact
     1201 ttgctcgtgt cgccaccctc gcgggcttcg tcccggaggc gttcgaggag cgcgtcgagc
     1261 atcgatgtga acgctccgcg cgcctggaac gggggctgcg ccagcgccgc tccataccgc
     1321 gctgccattc caccccgtat ggcttgcagg agtcggtccg ctgcggcggc cccgctcccc
     1381 tcgtttccct cagtcgcaac tagcctgcca atcgctccct cggcaccctg tacccgccgc
     1441 gcgacgcttg cggcgtcccc taggcgtgac atcatgtgtt gctgtacgaa tgatgtcaca
     1501 acactgtctg gcaacctgcc tactctcacc cggaccgtcc gggagaggat cgtcggcaag
     1561 agcgcgtcgg gttcactcgt cgtcaggatg atgaaggctg tcggcggcgg ctcctcgagt
     1621 gccttgagca ccgcgtttgc cgctccgtcc tgacccttct gtgccgccaa acgctccgca
     1681 tcgcccaaga tgaagacggc ccgtgaaccc tggacgggcc tcagtgcgag acgcctgagc
     1741 atcaggcgcg ccgacgccat gctgtgcgcc gccagtcccg ccggggcagc gtagaaaggc
     1801 tcttcgcgac gctcggccag cacctcgccg atcgcctctt ccacgaggtc gatctgcttg
     1861 tctgcgtcag cgcctttctt cgtgagctca accgggatga accagtgcag atccgggtgg
     1921 ctcaggtttt cgacctgccg gcacgacgcg cattttccgc acggcgcaga cgccgtttcg
     1981 cagagaagcg tctgggccag ccacaacgcc aatcgctgct tcccgacccc cctggggccc
     2041 tcgaacaaca gagtctgtgg gagacggcca gcccggattg catcggcgaa gcgcgcccgc
     2101 agcgcttcgt ggccagcgat aggggggagt ggcattgcgg gaaatctagc ggcagggggc
     2161 ctgggcgacc cgatttcgtt tgtcaactca ttacgctaca atggtatatc actactggac
     2221 aggtattgcg ctactataat agtatgtgca ataatgagtt atgtgaaaaa gtgagcacac
     2281 aatcgccgat ttcgtctgat aattgctagt ttcttcggtg tacatacggg atttattgac
     2341 ctggattcag taccggctcg atgtgtttct cgtagcaatc cgggcaaatg ccgtggctga
     2401 attccgcacg cgaacgctgt tcgatgtagg tctcgatcga tgtccaacgg ttcatttcat
     2461 cccgaatccg tttgcagtag ctgcaaatcg gaagcagacc ctccagctgc tgcacttccc
     2521 tccgaagtcc gaggatacgc tccgcgaccc gaagccggca ctgcaggtcg acggcgtcga
     2581 gcggcttggc gaggaagtcg tcgacccccc catccatcgc ttcgaggtac tcggcccgcc
     2641 cgcttttcgc cgtcagcatg atgaaatacg tgtactctcc cgagcgactt cggatcttcc
     2701 ggcacagatc cagtccctcc atctgcggca tctcccagtc ggaaatgacc accgggatcc
     2761 ggatagactg caggacttcc caggcttcgg ccccatccgc agcgtcggta actgcgtggc
     2821 cggcggccag gagcagccgc ttgaggactt cccgggtgac agactcgtct tcgacgatga
     2881 ggatttgcac gtccctggtc gctccggtct attggggccg tcgatcgagt gcggattgca
     2941 ccagccgctc cagtgtttcc gtttcgaagg gcttggagag aaaggcgcca cccgtggcgg
     3001 cggcttcgtg ggccaaaccc tggtccgcgt accccgacat atacacgacg ggcagcccgg
     3061 ggcgcgactc ccgcagacgg cgcgccaggt cggggccgct catgccgggc atgaccacgt
     3121 cggtgacgag caggtcgagc gtcagggcag ggtccgaggc gagctccagg gcgacctgcc
     3181 cgtcggtcgc cgacagcacc cggtagccac gagcctccag tacccgctgc gtcagcagcc
     3241 gaacccgcgg ctcgtcctcc gccaggagga tctgcgcagc gggcgcgcgc ttctcaccgg
     3301 agtccgccgg aaccgccgcc ggcagggagg cccgccggaa actggcgtcc tcgacccgag
     3361 ggagaaacac ggagaacgtg ctgccctttc cgggcgctga ctcgaccgag atggacccgc
     3421 cactctgccg cacaatcccg tacaccgtgg ccagccccag ccccgttcct ttgccgacct
     3481 ctttggtggt gaagaacggt tcgaagaggt gggaccggac tcggtcatcc attccaatgc
     3541 ccgtgtcggt gatcgccagc atcacgcgcg acaccggttg atcggaggtc ccgggaacgg
     3601 gggtgtcccg ccggttggcc gtccggatgg tgaggcggcc tccctggggc atggcgtccc
     3661 gggcgttcac ggcgagattc agcaacacct gctcgatctg gcctgggtcg gccatgaccc
     3721 gcccgaggtc ctcccccaga atggtcacga gctccacgtc ttcgccgatc agccgctgca
     3781 gcatcttgtc catctcgctg acgacgacgt tcaggtccag cacctccggc tggagcacct
     3841 gccgccgcga atacgcgagg agctgccggg tcagtcccgc ggccctcgcg gccgagcgct
     3901 ggatttcgtc gacatcttca cgggaccgcg atccggcggg gaggtcccgc tgcat
//
LOCUS       DASUBP010000142         3888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_303478, whole genome shotgun sequence.
ACCESSION   DASUBP010000142 DASUBP010000000
VERSION     DASUBP010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_303478"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(29..349)
                     /locus_tag="VFU75_05280"
     CDS             complement(29..349)
                     /locus_tag="VFU75_05280"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEU5466280.1"
                     /translation="MEAYRDQYATLLKDGKKVVVIGISMDADTTLHAWMHDEDFPFVF
                     VSDTGGAVGQKYGAYSVENKIDVRYLFVIDGSGKITHVIAPFHVLDPADYNTMKAAVD
                     SVTK"
     gene            complement(359..598)
                     /locus_tag="VFU75_05285"
     CDS             complement(359..598)
                     /locus_tag="VFU75_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466281.1"
                     /translation="MQRVMMLGVAGLLLAGGLSAQTPAPKPAPPTPLAVGTQAPDFAL
                     PGATRYGLMARPVHLSDFKGKTVVLAFFYEARTKG"
     gene            complement(633..872)
                     /locus_tag="VFU75_05290"
     CDS             complement(633..872)
                     /locus_tag="VFU75_05290"
                     /inference="COORDINATES: protein motif:HMM:NF020967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HEU5466282.1"
                     /translation="MKPVFSARDPVEAHLLRAVLESEGIEAKVMGEFLYLARGGVPFD
                     NHTEPSVWVSDEDVDRARTVVEAFVRRRDLTIEPD"
     gene            complement(869..2710)
                     /locus_tag="VFU75_05295"
     CDS             complement(869..2710)
                     /locus_tag="VFU75_05295"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HEU5466283.1"
                     /translation="MASPPTLRALIDDGRVHVFDGAMGTQLYAQGFYLNVCYDELALK
                     QPALVRSVHEAYVAAGAELLETNTFGANPPKLAAYGLADQTERINRAAAELARAAARD
                     QASVVGAIGPLGLRLEPFGPTSSADARAHFARQVKGLLDGGVDGFIIETFSDLAELKA
                     ALDAVRESTDLPVVAQMTVGTDGLTYLGTAPEDFAKTLTEWGADVIGVNCSVGPAGVL
                     DAIERMAKVTDRPLSGQPNAGLPKAVEDRKIYLASPEYVASYAKRMVEAGARFVGGCC
                     GTTPEHIRRIRAYVASVSPRRHATVVSRTAVTPPAGVTEVPLAERSRWGAKLARGEFV
                     TTVEILPPKGVDPSPMLAQCRALKAAGVDAVNIPDGPRAMSRMGVIPSALLIERETGI
                     ETVMHYCCRDRNLLGMLSDLLGAAAAGLHNVLIITGDPPKMGPYPEATAVFDIDSIGL
                     TNLVYRLNQGLDPGHNPIGSPTRWAIGVGVNPGAPDLDRELSRFAWKVDAGADFAVTQ
                     PVFDPHQLETFLKRVESFRIPVVAGIWPLVSLRNAEFLANEVPGVSVPEEILRRMRDA
                     QEKGREAALSEGVKVARDMLAGVRGAVQGVQVSAPFGRVEVALDVIK"
     gene            2764..3597
                     /locus_tag="VFU75_05300"
     CDS             2764..3597
                     /locus_tag="VFU75_05300"
                     /inference="COORDINATES: protein motif:HMM:NF021505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2231 domain-containing protein"
                     /protein_id="HEU5466284.1"
                     /translation="MPNIGFFHPIIVHFVIALLYVGVAFRLVSLTGKLQFTNFGALTL
                     ILIGTVAAVLAVQSGQDAHGAVERIPGLRSAVGSHEDWAHDARNAFLVVSLIEILAFV
                     FAKRSFHRVLLFASGLAGAAACIVLFIAADKGGDIVYEYAGGPGLRSGDTADVSRAYL
                     AGLYNEAMLERKNGKNADAAALIDEMSKRFPNDTTVRLLTIESLIVDRKDGKGALALL
                     KGFSAPADGSRFLRFRTLLLKSDAYQAAGFADSARAVLEGAPAEFQNNPQVKNRLDKL
                     K"
     gene            complement(3681..>3888)
                     /locus_tag="VFU75_05305"
     CDS             complement(3681..>3888)
                     /locus_tag="VFU75_05305"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEU5466285.1"
                     /translation="IDVLILALVVRFFGSLLGAFQFNRWTRWAYVLTNWMIEPLAKVL
                     PQYANVDWSPLAAFVLLLIVKAIL"
BASE COUNT          661 a         1285 c         1278 g          664 t
ORIGIN      
        1 tcccacctcc ccacgccttc ttgtcgggct acttcgttac cgagtctacc gcagccttca
       61 tcgtgttgta gtcggccgga tcgagcacgt ggaacggtgc gatcacgtgg gtgatcttgc
      121 cgctcccgtc gatcacgaac aagtatcgga cgtcgatctt gttctcgacc gaataggcgc
      181 cgtacttctg gcccaccgca ccgccggtat ctgagacgaa cacgaacggg aagtcttcat
      241 cgtgcatcca ggcgtggagc gtcgtgtcgg catccatgct gatgccgatg accaccacct
      301 tcttcccatc cttcagcaac gtagcgtact gatcacggta cgcttccatt tggatggttc
      361 agcctttggt gcgcgcctcg tagaagaagg cgaggacgac ggtcttgccc ttgaagtccg
      421 acaggtgcac cggccgggcc atgaggccgt agcgggtggc gccgggaagg gcgaagtccg
      481 gagcctgggt tcccaccgcc agcggggtgg gaggggcggg cttgggcgcc ggcgtctgcg
      541 cgctcaagcc accggcgagc agcaggccgg cgacgcccag catcatgact cgttgcaact
      601 gaactccctg atggggacac cgggaatgta agtcagtccg gctcgatggt gaggtcacgg
      661 cgcctgacga aggcctccac cactgtccgg gcccggtcaa catcctcgtc ggacacccag
      721 acggacggct cggtatgatt gtcgaagggg acgccgcccc gggccagata caggaactct
      781 cccatcacct tcgcctcgat accttcactc tcgagcaccg cgcggagcag atgcgcctcc
      841 acgggatcgc gggccgagaa gaccggtttc acttgatgac gtccagcgcg acctccacgc
      901 ggccgaaggg ggcgctcacc tgcacgcctt gcacggcccc tcgcacgccc gccagcatgt
      961 cccgagcgac cttgacgccc tctgacagtg ccgcctcccg tcctttctcc tgcgcgtccc
     1021 gcatccgccg gagaatctcc tcgggtaccg aaactcccgg aacttcgttc gccaggaatt
     1081 cggcgttccg gaggctcacc agcggccaga tgccggccac caccggaatg cggaagctct
     1141 ccacccgctt caggaacgtt tcgagctggt ggggatcgaa caccggctgc gtgaccgcga
     1201 aatcggcgcc cgcgtccacc ttccacgcga atcgcgacag ctcccggtcc aagtcgggcg
     1261 cgcccggatt gacgcccacc ccgatcgccc acctcgtcgg cgacccgatc gggttgtgac
     1321 cgggatccag gccctggttg agacgatata ccaggttcgt gagtccgatc gagtcgatgt
     1381 cgaagaccgc cgtcgcctcc gggtagggcc ccatcttcgg cggatcaccg gtgatgatca
     1441 ggacattgtg gagccccgcg gcggccgcgc cgaggaggtc cgacagcatg cccaggaggt
     1501 tccggtcccg gcagcagtag tgcatcacgg tttcgatgcc ggtttcccgt tcgatgagga
     1561 gtgcggatgg aatcacaccc atccggctca tcgcgcgggg tccgtcgggg atgttcaccg
     1621 cgtcgacacc ggcggccttc aacgcccggc attgcgcgag catgggtgac gggtcgacac
     1681 ccttgggcgg cagaatctcc accgtcgtga cgaactcgcc gcgcgcgagc ttcgctcccc
     1741 agcgcgaccg ctcggccagc gggacttcgg tgacccccgc gggaggagtc actgcggtcc
     1801 gcgagacgac ggtcgcgtgg cggcgcgggg aaacgctggc gacatacgcc cggatgcgcc
     1861 ggatgtgctc cggcgtggtt ccgcagcagc cacccacgaa ccgggcaccc gcctcgacca
     1921 tccgcttggc atagctcgcg acatactcgg gagacgccag gtagatcttc cggtcttcga
     1981 cggccttcgg gagcccggcg ttgggttggc ccgacagggg acggtcggtg accttcgcca
     2041 tccgctcgat ggcatcgagc acgcccgcag gtcccaccga gcagttcact ccgatgacgt
     2101 cggcacccca ctccgtgagc gtcttggcga aatcctcggg ggcggtgccg aggtaggtca
     2161 atccatcggt gcccacggtc atctgggcga cgacggggag gtcggtcgac tcccgtaccg
     2221 cgtcgagcgc cgccttgagc tcggccagat cgctgaaggt ttcgatgatg aacccgtcga
     2281 cgccgccatc gaggaggcct ttcacctgcc gggcgaaatg cgcgcgcgca tccgcgctgg
     2341 aggtagggcc gaagggttcc agacggagac cgagtggccc gatagcgccc accacggagg
     2401 cctggtcgcg ggcggcggcg cgagcgagct cggcggcggc ccggttgatg cgctcggtct
     2461 gatcagccaa cccgtaggcg gcgagcttgg gcggattggc gccgaacgtg ttggtctcga
     2521 ggagctcggc ccccgccgcg acgtaggctt cgtgaaccga gcgcaccagg gcgggttgct
     2581 tgagggccag ctcgtcgtag cagacgttca agtagaaacc ctgggcatag agctgcgtgc
     2641 ccatggcgcc gtcgaagacg tggacgcgcc cgtcgtcgat cagggcgcgg agggtaggag
     2701 gggaggccac ggtgttgccc gcaatctaat agctttagtg accaccaact cgggcccaac
     2761 tccgtgccga acatcggctt ctttcacccc atcatcgtcc acttcgtcat cgcccttctt
     2821 tacgtcggcg tggcgttccg gctggtctcc ctcaccggca agcttcaatt caccaatttc
     2881 ggagcgctga cgctgatcct gatcggcacc gtcgcggccg tgctcgcggt gcaatccggg
     2941 caggacgctc acggcgccgt ggaacggatt cccggccttc ggtcggcggt cgggtcccat
     3001 gaggactggg cccacgatgc ccgcaacgcg ttcctggtcg tgtcactcat cgaaatcctg
     3061 gcgttcgtgt tcgccaagcg ctccttccat cgggtcctgc ttttcgcctc cgggctggcg
     3121 ggcgcggcgg cgtgcatcgt gctcttcatt gccgctgaca agggcgggga catcgtgtat
     3181 gagtacgccg gcggacccgg cctgcggagc ggggacacgg cggacgtcag ccgggcgtac
     3241 ctggcggggc tctacaacga agccatgctg gagaggaaga acgggaagaa cgcggacgcg
     3301 gcggcgctga tcgacgagat gtcgaagcgc ttcccgaatg acaccacggt tcgtctcctc
     3361 accatcgaat ccctgatcgt cgaccggaag gacggcaaag gcgcgctggc gctgctgaag
     3421 gggttctccg caccggcgga cgggtcgcgg ttcctgcgct tccgcacgct gctgctcaag
     3481 tccgacgcgt atcaggcggc gggattcgcg gattccgccc gcgccgtgct ggagggcgca
     3541 ccggctgaat tccagaacaa cccgcaggtg aagaaccggt tggacaaact gaaatagcgg
     3601 gcgtgccgcg agcgaggtga tcgggatcgg cacgggtggg gcggtgtcga tcccgtcgtt
     3661 tgcctatttg ctccgcgtgt tcaaaggatc gccttcacga tgagcaagag cacgaaggcc
     3721 gcgagcgggc tccaatctac gttggcatac tgcggcagga ctttcgcgag gggctcgatc
     3781 atccagttcg tcagcacgta cgcccaccgc gtccaccggt tgaactgaaa ggcgcccagc
     3841 agggaaccga agaaacgcac gaccagcgca agaataagaa cgtcgatg
//
LOCUS       DASUBP010000143         3386 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_439650, whole genome shotgun sequence.
ACCESSION   DASUBP010000143 DASUBP010000000
VERSION     DASUBP010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3386)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3386)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3386
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439650"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2012)
                     /locus_tag="VFU75_05310"
     CDS             complement(<1..2012)
                     /locus_tag="VFU75_05310"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU5466286.1"
                     /translation="MARLKHLLALTLPVSLFPLPAVAQKRAITIDDYLALKNVGDPQV
                     SPDGKWVVYTVGTISLQDNKSTSRLWLANAATGATRELSQGAGSDRSPRWSPDGQTIA
                     FLSAREHGPQVWVMPAAGGVARRVSDLPEGVGEMYWMPDGRGFLVVSDVKWPKAQEID
                     QRNGAYPTDARLWTNVLYRHWTDWRAGMRQHVFSVDLPSGAAKDLTPIDKDAPTIATS
                     GDGDVAMSPDGREIAVAIHADSVVADNTNVDIYLMGPDGSGMRDLTAANAGADNTPRF
                     SPDGKWLSYLSMARAGFEADRLRLMLVPRSAGRTDGAPVEATAGWNLSVTSYSWCADS
                     KCIYAVVEERGRDNLYRIALPGFQRTLLFGKNGVNTNLTVSNDGKTRAWLYQSNTQPA
                     EVFVNDKALTHTNDDLLSRLDLHPLESYGFVGAGGDSVFGWLLKPPGFDATKKYPVIY
                     VIHGGPQGAWDDNWHARWNYQMFASRGYVVAAVNFHGSTGYGQAFTDAISQHWGDWPY
                     EDVMKGLDVVARLPYVDSTRMGAAGASYGGYMVYWLAGHTNRFRVLVDHDGVFNPESM
                     FGTTEELWFPKWEFGGTPYQEAGGNRALYEQWSPLNFVQNWKTPMLIVHSQLDYRVDL
                     SEGYQAFTALKLHNVPGKFLYFPDEGHFVVRPRNRRLWWGVVLDWM"
     gene            complement(2071..2328)
                     /locus_tag="VFU75_05315"
     CDS             complement(2071..2328)
                     /locus_tag="VFU75_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466287.1"
                     /translation="MDLAITRAQKDAVLGAKHLPDVLKRVLDQAKAQGSDFVVHMTYE
                     EATALNELCSWNVHTDAAGNVTPDSKLFDDLVKAIITHPDY"
     gene            2400..>3386
                     /locus_tag="VFU75_05320"
     CDS             2400..>3386
                     /locus_tag="VFU75_05320"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEU5466288.1"
                     /translation="MPRTEFLATGFHVPEKVVTNDDLSRMMDTSDDWIVQRSGIKTRF
                     WVSPGETGVGLAAEASRRALAAAGMTPKDVDCIVYCTCTPDHFEPGNGVFLQRELGIA
                     DIPAVDVRNQCSGFIYGLSIADAWIRIGQYRRILLVGAEVHSRGLDKTTRGRDTAVLF
                     GDGAGAVILGPTEDPGRGVLSTHLYSDGRHAEKLWVDGPGLAHDPYVSVEMIEQGLHR
                     AVMEGREVFKFASLKMPESVSVALSTNGLSTKDITLLVPHQANLRIIEMVRQATGLTE
                     AQVYANIQKYGNTTAASIPIALHEALGEKRLKRGDLLVLTAFGSGFTWGSAAI"
BASE COUNT          574 a         1111 c         1094 g          607 t
ORIGIN      
        1 tccatccagt cgagcacgac tccccaccac agacgccgat tccggggccg caccacgaag
       61 tgcccctcat cggggaagta caggaatttg ccggggacgt tgtgaagctt cagggccgtg
      121 aacgcctggt agccctcgga caggtccacc cgatagtcga gctgggagtg gacgatcagc
      181 atgggggtct tccagttctg gacgaagttc agcggagacc attgctcgta cagagcccga
      241 ttcccccccg cttcctggta aggcgtcccc ccgaactccc acttggggaa ccacagctct
      301 tccgtggtgc cgaacatgga ctcggggttg aagacaccgt catggtccac cagcacccgg
      361 aaccggttgg tgtgaccggc cagccagtag accatgtacc cgccgtagga tgcccccgcc
      421 gctcccatcc gcgtggaatc cacgtacggc aggcgggcca cgacgtcgag ccccttcatc
      481 acgtcttcgt acggccagtc gccccagtgt tgagagatgg cgtcggtgaa cgcctgcccg
      541 tagccggtgg agccgtggaa gttgacggct gcgacgacgt acccgcgcga cgcgaacatc
      601 tggtaattcc accgggcgtg ccagttgtcg tcccacgcac cctgcgggcc gccatggatg
      661 acgtagatga cgggatactt cttcgtcgcg tcgaatccgg gcggcttcag aagccagccg
      721 aatacggaat ctccacccgc tccgacgaat ccgtaggact cgagtggatg caggtcgaga
      781 cgcgacagca gatcatcgtt ggtgtgcgtg agcgccttgt cgttgacgaa tacctcggcg
      841 ggctgggtgt tgctctggta gagccacgcc cgagtcttgc catcgttcga cacggtgaga
      901 ttggtgttga cgccgttctt cccgaagagc agcgtgcgct ggaatcccgg cagtgcgatg
      961 cgatagaggt tgtcccgtcc ccgctcctcc accaccgcgt aaatgcactt ggaatcggcg
     1021 caccaggaat acgaagtcac ggagaggttc cacccggccg tcgcctcgac gggcgcaccg
     1081 tcggtccgtc cggcactccg cggaaccagc atcaggcgca ggcggtccgc ttcgaagccg
     1141 gcgcgcgcca tggacaggta cgacagccac ttgccgtccg gcgagaagcg cggcgtgttg
     1201 tcggcgcccg cattcgcggc cgtgaggtcg cgcatcccgc tgccgtcggg gcccatcagg
     1261 tagatgtcga cgttcgtgtt gtcggcgacc acggagtcgg cgtgaatcgc caccgctatt
     1321 tcccggccat cgggagacat ggcgacgtcc ccgtcacccg aggtcgcgat cgtcggcgcg
     1381 tccttgtcga tgggcgtgag atctttcgcg gcgcccgacg gcagatccac gctgaacacg
     1441 tgctgccgca ttcccgcccg ccagtcggtc cagtgccggt agagcacgtt ggtccacagc
     1501 cgcgcgtcgg tgggataggc gccgttccgt tgatcgattt cctgcgcctt gggccatttg
     1561 acgtccgaga ccaccaggaa acccctcccg tcgggcatcc agtacatctc gccgacgccc
     1621 tcaggcagat cgctcacccg ccgtgccacg cccccggccg ccggcatcac ccagacctgc
     1681 ggcccatgct cgcgtgccga gagaaaggcg atcgtctgcc cgtccgggga ccagcgcgga
     1741 gagcggtccg agcccgcccc ctgcgacagc tctctcgtcg ctcccgtcgc ggcattggcg
     1801 agccagagtc tcgaggtgga cttgttgtcc tgaagtgaga tcgtcccgac cgtgtacacc
     1861 acccatttcc cgtccggaga cacctgtgga tcgccgacgt tcttcagcgc caggtagtcg
     1921 tcgatggtga tggcgcgctt ctgggcaact gcgggaagcg ggaaaaggga aacgggaagc
     1981 gtgagtgcca gcagatgctt caggcgtgcc atgggatctc cagtgtagac cttgccgact
     2041 tggcgactta gcgacttggc gtcttagcga ctaatagtcc gggtgagtga tgatcgcttt
     2101 cacgagatcg tcaaacagct tggaatccgg cgtcacattg ccggcagcgt cggtgtggac
     2161 gttccacgag caaagctcgt tcagcgccgt ggcttcttca taggtcatgt gaaccacgaa
     2221 gtccgagcct tgggccttcg cctggtccag gacccgtttc aggacgtcgg gaaggtgctt
     2281 ggcgcccagg accgcgtcct tctgcgcccg cgtgatggcg agatccatgt cccttgcttt
     2341 gcggatgagg acgcgaaatc ttctccgccc cgtcccatcc cgcaaccgga gaaggttcga
     2401 tgccgcggac tgagtttctg gctaccggtt tccacgtgcc ggagaaggtc gtcaccaacg
     2461 acgatctgtc ccggatgatg gacacctcgg acgactggat cgtccagcgc agcgggatca
     2521 agacgcgctt ctgggtgtcc cccggggaga ccggggtcgg tctcgcggcc gaggcttccc
     2581 ggcgcgcgct cgcggccgcg gggatgaccc cgaaggacgt ggactgcatc gtctactgca
     2641 cctgcacccc cgaccacttc gagcccggga acggcgtgtt tctccagcgc gagctcggga
     2701 tcgcggacat tcccgccgtc gacgtccgca atcagtgcag cgggttcatc tacgggttgt
     2761 ccatcgccga cgcctggatc cggatcggcc agtatcgccg cattctcctg gtcggtgcgg
     2821 aggtgcattc gcgcggcctg gacaagacca cgcggggacg ggataccgcg gtgctcttcg
     2881 gtgatggtgc cggcgccgtc atcctcggcc ccacggaaga ccccggccgc ggcgtgctct
     2941 ccacccacct ctattcggac ggccggcatg ccgagaagct ctgggtggac ggacccggcc
     3001 tggcccacga tccctacgtg tcggtggaga tgatcgagca ggggctgcac cgggccgtga
     3061 tggaggggcg ggaggtcttc aagttcgcgt cgctgaagat gcccgaatcc gtgtccgtgg
     3121 cgctgtcgac caacgggctc tcgacgaagg acatcaccct cctcgtcccc caccaggcga
     3181 acctccggat catcgagatg gtgcggcagg ccaccgggct caccgaggcg caggtctacg
     3241 ccaacatcca gaagtacggc aacacgacgg ccgcgtcgat tcccatcgcg ctccatgagg
     3301 cgctgggcga aaagcgactg aagcgagggg acctgctggt tctgacggcg ttcgggtcgg
     3361 ggtttacgtg ggggagtgcg gcgatc
//
LOCUS       DASUBP010000144         5240 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1025379, whole genome shotgun sequence.
ACCESSION   DASUBP010000144 DASUBP010000000
VERSION     DASUBP010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5240)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5240)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5240
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1025379"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFU75_05325"
     CDS             <1..351
                     /locus_tag="VFU75_05325"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase"
                     /protein_id="HEU5466289.1"
                     /translation="WAEVTGAWDIFLGVNALDYSGYPDCRPEYIAAFERMANLGTKAG
                     VEGDGFTVHTPLIQMTKKEIVAKGLELGVDFGMTVSCYDPTPGPVACGHCDACLLRLR
                     AFEELGKKDPARYR"
     gene            348..980
                     /gene="queE"
                     /locus_tag="VFU75_05330"
     CDS             348..980
                     /gene="queE"
                     /locus_tag="VFU75_05330"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase"
                     /protein_id="HEU5466290.1"
                     /translation="MSYAVKEIYYTLQGEGANTGRPAVFLRFAGCNLWTGREKDRQNA
                     ICRFCDTQFVGTDGLGGGSFDTPHALADAVTAKWEGEATLKLVVCTGGEPLLQLDEPL
                     IEALHRARFMVAVETNGTQVVPRGIDWVCVSPKAEADLIVKTGDELKLVYPQDGGDPE
                     RYGDLAFTHFYLQPMDGPDRERNTELAMAYCLAHPRWRLSLQTHKYLGIP"
     gene            982..1341
                     /gene="queD"
                     /locus_tag="VFU75_05335"
     CDS             982..1341
                     /gene="queD"
                     /locus_tag="VFU75_05335"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HEU5466291.1"
                     /translation="MEIFKEFTFEAAHHLPNVSPTHPCARVHGHSFTLRVSIRGPLDG
                     KAGWVVDFADIIEATAPMIAELDHHDLNEIPGLENPTTELLARWVWKRLKSQLPGLSR
                     IEIRETATTGCVYDGSD"
     gene            complement(1360..1863)
                     /locus_tag="VFU75_05340"
     CDS             complement(1360..1863)
                     /locus_tag="VFU75_05340"
                     /inference="COORDINATES: protein motif:HMM:NF019241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5466292.1"
                     /translation="MKVAWIAAAFARDLKSLKREIAAYPNERDLWKVVPGISNTGGNL
                     ALHLAGNVRYFIGAVLGANGYIRDRNAEFSRKDVPRAEVLTEIEAAFAAMQKGMGHVT
                     EADLGKPYGQPIGGQQLTIGDALLHLVTHLTYHLGQIDYHRRIVTGQNVAVGTISVGE
                     MATAKPA"
     gene            complement(1878..2630)
                     /locus_tag="VFU75_05345"
     CDS             complement(1878..2630)
                     /locus_tag="VFU75_05345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466293.1"
                     /translation="MKMKLGKLAPRHDPRTLLFATYFRDQALPPPPSSEDWGSRIKTW
                     PMMANDSIGDCTTAAAGHMIEQWTTYAGKTTTPSDTAVIAAYSAVSGYNPRTGANDNG
                     AVEIDVLNYWRHTGIAKHKIMAYVALEPKNHTQVMDAVLLFGNCYIGLALPQTAQTQA
                     VWTIPPGGPSGAGAPGSWGGHAVPIVAYDERGLTCVTWGMLKRMTWTFWDAYCDEAYA
                     VLSRDWVNQASGQAPNRFDLAALQKDLADLNA"
     gene            2808..3758
                     /locus_tag="VFU75_05350"
     CDS             2808..3758
                     /locus_tag="VFU75_05350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466294.1"
                     /translation="MRRVMAVLAVFAFPVLAGLGHAQIVRTALHVVTANPKGAVKVAT
                     LAFAVDGGGLRYDPATDDFRGTLELWLEDQDSAGLGYTLPQPVTINLFSEADSLSEGL
                     AHFTGTEQPQRISIVARSPGDSVKIKLWPLARIDSVTAMVRVLPALRLQVTPTQVAAW
                     GLETAVATVELLGDAHGEFGVTVRPTRADPMTLQFTHRDAKSAVLRSRGLGADTVRVS
                     GAGLAPSMTVVTYVFPFALLIALALGSTIGTTALQLSSKTRSTAPWWAFLRGLLLGLV
                     AVAAYAVGVKAIPVPIPAQVGEGGSFVVAALASYLGLRRR"
     gene            3809..4531
                     /locus_tag="VFU75_05355"
     CDS             3809..4531
                     /locus_tag="VFU75_05355"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5466295.1"
                     /translation="MNLRLAVLVAGGALAASPLSAQLSARDTSEFLTVTQALLDAITT
                     GDTSVWAPHLAPDWFATDEEGNRLSRADFLAQMHPLPAGQSGVLKVGAWHLIGHADWA
                     VFTYDIEETHHYYDQTLRTRFHSTDTYARTAGRWWQVATQETALPTPVAGVTVDPARL
                     APLAGTYALTPSISLSISVRDSSLWMASGSRPAQRLYALSDQLFVVHGRRGVWVFERG
                     AAGNVTNLVYWRDNNGVRWRRN"
     gene            4635..5099
                     /locus_tag="VFU75_05360"
     CDS             4635..5099
                     /locus_tag="VFU75_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466296.1"
                     /translation="MRKALSAGIGAIGFVAACGTKPLPTSGGGLGGINGRDQISALWD
                     TSDTALTSSAEVESGYQFTVSFKTVAGGCLSPDYVAVQGGGSSVTVTPFDLNSGGAGL
                     MCPMSLTSAERSTQVVLTAVGLDTVRLRGMVGFGESARLVTVQQIIKVLPAP"
BASE COUNT          864 a         1695 c         1720 g          961 t
ORIGIN      
        1 tgggctgagg tgaccggggc gtgggacatc tttctgggcg tgaacgcgct cgactactcc
       61 ggctacccgg actgccggcc cgagtacatc gcggctttcg aacggatggc caacctgggc
      121 accaaggcgg gcgtggaagg cgacggattc acggttcaca cgccgttgat ccagatgacg
      181 aagaaggaga ttgtggcgaa aggactcgag ttgggcgtgg atttcggaat gacggtgagc
      241 tgctacgatc ccacgccggg accggtggcc tgcggccact gtgacgcctg cctcctccgg
      301 ctccgcgcgt tcgaggagct cgggaagaag gacccggccc ggtaccgatg agctacgccg
      361 tgaaggagat ctactacacg ctccaggggg agggcgcgaa cacgggccgt cccgcggtgt
      421 ttctccggtt cgccgggtgc aacctctgga cgggtcgtga gaaggaccgg cagaacgcca
      481 tctgccggtt ttgcgacaca cagttcgtcg gcacggacgg gcttggtgga ggctcgttcg
      541 atacgcccca tgcgctcgcg gatgcggtga ccgcaaagtg ggaaggggaa gcgaccctga
      601 agctggtggt gtgtaccggc ggggagcccc ttcttcagtt ggacgagccg ctgatcgagg
      661 ctctgcatcg ggcccgattc atggtcgccg tggaaaccaa cggcacgcag gtcgtgccgc
      721 gcggcatcga ctgggtgtgt gtgagcccga aggccgaggc ggatctgatc gtgaagacgg
      781 gggacgagtt gaagctggtc tatccgcaag atggcgggga ccccgagcgt tatggcgacc
      841 tcgcgttcac gcacttttat ctgcagccca tggatggccc ggaccgcgag cggaatacgg
      901 agctcgccat ggcctactgc ctggcgcatc cgcggtggcg gttgagcttg cagactcaca
      961 agtatctggg gattccctga cgtggagatc ttcaaggagt tcacgttcga ggccgcgcat
     1021 cacctgccca acgtttctcc gacccatccg tgcgcgcggg tgcacgggca ctcgttcacg
     1081 ctccgggtct cgattcgcgg tccgctggac gggaaggccg gctgggtggt ggacttcgcc
     1141 gacatcatcg aggcgacggc gccgatgatc gccgagctcg accatcatga cctgaacgag
     1201 attcccggac tcgagaaccc cactacggag ctgctggcgc ggtgggtttg gaagcggctc
     1261 aagtcccaat tgccaggact gtcgcggatc gaaatccggg aaaccgcgac gaccggctgc
     1321 gtctacgacg gatccgacta gccgtctagc cgtctagtct tacgccggtt ttgccgtcgc
     1381 catctcgccg accgaaatcg tcccgaccgc gacgttctgc cccgtcacga tgcgccggtg
     1441 atagtctatt tggcccagat ggtaggtgag atgcgtgacg aggtggagca gggcgtcacc
     1501 gatggtgagc tgctgcccgc cgatcggttg tccgtacggc ttgccaagat ccgcttccgt
     1561 aacgtgtccc atgcccttct gcattgccgc aaacgccgct tcgatctccg tcagcacctc
     1621 ggcgcgcggc acatccttcc ggctgaactc cgcattgcgg tcccggatgt agccattcgc
     1681 gccgaggacg gcgccgatga agtagcggac gttcccggcc aggtgcaggg ccagattgcc
     1741 gcccgtgttg ctgatcccgg gcacgacctt ccagaggtca cgctcgttcg ggtaggcggc
     1801 gatctcacgc ttgagggatt tcaggtcacg ggcgaaggcg gcggcgatcc aggcgacttt
     1861 catcccccaa aaatgcccta ggcgttcagg tccgccagat ccttctgcaa cgccgccaaa
     1921 tcgaaacgat tcggcgcttg cccgctcgcc tgattgaccc agtccctcga caggaccgcg
     1981 taggcctcgt cgcaataggc atcccagaac gtccacgtca tccgcttgag catgccccag
     2041 gtcacgcagg tgagcccccg ctcatcgtac gcaacgatcg gtacggcatg tccgccccac
     2101 gatcccggag cgccggcccc actcgggcca ccggggggaa tggtccatac ggcctgcgtc
     2161 tgggcggtct gcggcagcgc caacccgatg tagcaattcc cgaaaagcag gacggcatcc
     2221 atgacctgtg tgtggttctt cggttcgagc gcgacatagg ccatgatctt gtgcttcgcg
     2281 atgcccgtgt ggcgccagta gttcagcacg tcgatctcca ccgcgccgtt gtcgtttgcc
     2341 ccggtccgcg gattgtagcc cgagaccgcc gagtaggcgg cgatgacggc ggtgtcggag
     2401 ggagtcgtcg tcttccccgc gtacgtcgtc cactgctcga tcatgtgccc cgcggcggcg
     2461 gtggtgcagt cgccgatcga gtcattggcc atcatcggcc aggtcttgat ccgcgacccc
     2521 cagtcttcgg atgacggtgg aggaggcagg gcctggtctc tgaaataggt ggcgaagagc
     2581 agggtacgcg gatcgtgacg cggggcgagt ttgccgagct tcatcttcat ggagccctct
     2641 cgttggggat ggatgcttta ccccgcagcg aattgccccc ggaccgtttt tgcgacatcg
     2701 agggtcgcga atgcgacacg cacgcatttc ggatgtcgca aaaacggcct atgcctgccg
     2761 caaaaacgcc cctgatccgt ttggaacggc cggggtattt ccgcaccatg cgtcgcgtca
     2821 tggccgtcct cgcagtcttc gcgttccccg tactggccgg actcggccac gcgcagatcg
     2881 tacgcaccgc gctgcacgtc gtgacggcca atcccaaggg cgccgtcaag gtcgcgacgc
     2941 tggcgttcgc ggtcgacggt ggcgggctcc gctacgatcc cgcgaccgac gacttccgcg
     3001 gaaccctcga actgtggctc gaggatcaag acagcgccgg cctcggctat acgcttccgc
     3061 aacccgtgac gatcaacctc ttctcggaag cggacagcct gtccgaagga ctcgcgcact
     3121 tcaccgggac ggaacagccc cagcgcatct cgatcgtcgc ccggtccccc ggagattccg
     3181 tgaagatcaa gctctggccc ctcgcgcgaa tcgattcggt caccgcgatg gtgcgcgtgc
     3241 tccccgcact gcgcctgcag gtgactccga cgcaggtcgc ggcgtgggga ctcgagaccg
     3301 cggtggcgac cgtcgagctt ctgggcgacg cccacggcga gttcggcgtg acggtgcgcc
     3361 ccacgcgcgc cgatccgatg actttgcagt tcacgcatcg agacgcgaag tccgcggtgc
     3421 tgcgctcccg agggctcggc gccgacaccg ttcgggtttc gggcgccggg ctcgcgcctt
     3481 ccatgacggt cgtgacctac gtcttcccgt tcgcgctcct catcgccttg gccctcggca
     3541 gcacgatcgg caccacggcc cttcaactct cctcgaaaac gagatccacg gcgccctggt
     3601 gggccttcct ccggggactg cttctgggat tggtggcggt cgcggcctac gccgtcggcg
     3661 tcaaggcgat tccggttccc atcccggcgc aggtggggga gggtggctcg ttcgtggtcg
     3721 ccgcgctggc atcgtatctg ggcctgcgga ggcggtagtt caccgggccg cacttccctc
     3781 ccggccgtct gcgaacaact atctcaccat gaacctccga ctcgcggtgc tcgtcgccgg
     3841 aggcgcgctc gccgcctctc cgctctccgc acagctcagt gcccgtgaca cgtccgagtt
     3901 tctcaccgtc acgcaagccc tcctcgacgc catcaccacg ggcgacacgt cggtgtgggc
     3961 accgcacctc gccccggact ggtttgccac ggacgaggaa ggcaatcgcc tgtcgcgagc
     4021 cgactttctc gcgcagatgc atccactccc cgcggggcag tcaggcgttc tgaaggtcgg
     4081 agcctggcac ctgatcggcc atgccgactg ggcggtgttc acgtacgaca tcgaagagac
     4141 gcatcactat tacgaccaga ccctccggac ccgctttcac tcgaccgata cctacgcccg
     4201 gacggccggc cggtggtggc aggtcgcgac gcaggagacc gcgctgccga cgccggttgc
     4261 cggtgtcacc gtcgacccag cccggctggc tcccttggcg gggacgtacg cgctcacgcc
     4321 atccatatct ctttccatct ctgtgcggga ttccagcctg tggatggcga gcggcagtcg
     4381 tcccgcccag cggttgtacg cgctcagcga tcagctcttt gtggtccatg ggcgccgggg
     4441 agtctgggta ttcgagcggg gagccgctgg gaacgtgacg aacctggtct actggcggga
     4501 caacaacggc gtccggtggc gccgcaactg accggtcagg ggctccactc gtcgcaggat
     4561 ccgtgcagat caccctgatc ggtgacgacg tactcacttg ataggtacac agctagagag
     4621 acctggaggg tccaatgcga aaggccttgt ctgcaggcat cggtgccatt gggttcgtcg
     4681 cggcgtgcgg gaccaagcca ctcccgacgt ccggtggcgg ccttgggggg atcaacggca
     4741 gggaccagat cagcgccctg tgggacacgt ccgacacagc cctcacttca tcggctgagg
     4801 tggagtccgg ataccagttc acggtgtcgt tcaagacggt ggccggtggt tgcctctcgc
     4861 cggattacgt ggcggtgcag ggtgggggat cgtcggtcac ggtgacgcca ttcgatctga
     4921 acagtggtgg cgccggtctg atgtgcccca tgtccctgac gtcggcggaa cggagcaccc
     4981 aggtggtact caccgcggtc gggctcgata cggtcaggct gcgggggatg gtgggtttcg
     5041 gcgagtcggc gaggctcgtg accgttcaac agatcatcaa ggtcttaccg gcgccatgaa
     5101 aacactgagc ggcccggcct cttcactagg atgaagaggg ggccgcccaa tcacaccgtg
     5161 gaccggagtt gaagcagcat gccgttcaga gactccgggc gtttaccccc atcgagtgcg
     5221 caatcgacgg ttgaccagtg
//
LOCUS       DASUBP010000145         9580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_249181, whole genome shotgun sequence.
ACCESSION   DASUBP010000145 DASUBP010000000
VERSION     DASUBP010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9580
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_249181"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1442
                     /gene="ggt"
                     /locus_tag="VFU75_05365"
     CDS             <1..1442
                     /gene="ggt"
                     /locus_tag="VFU75_05365"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU5466297.1"
                     /translation="GGFIVFRDSAGNARALDYRETAPAAATHNMYLDSAGNPTEASIT
                     GYQASGVPGSVAGMYEAWKKYGHLPWPKLIAPAIALAEGHVIDENRSRAIEYDRDRLG
                     MFAASAATFLVNGHAPAAGATVRYPELAKTLRLIADSGPSVFYRGQIADQIVAEMKRG
                     NGIITKADLAGYQAKWRTPIEVTYRGYRIYSMPPASSGGITMAEIFNILSGYDSLSAF
                     GSARYVHLEAEAMRRAFIDRNEWLGDPDFVKMPLDRLLSAAYASQLRSQILRDKATPT
                     PPFQTKPPESGQTTHYSVVDDHGNAVSVTTTLNNSFGSAVTVTGAGFLLNDEMDDFAS
                     APGKPNMFGLVQGEQNAIVPGKRMLSAMTPSVVLDPSGKLFMVVGTPGGPTIITSVYQ
                     VIANVIDQHMSLPAAVEAPRIHQQALPDVVFYERGGLSQAVVDSLTAMGYTMKERRGY
                     SGDIAAIEKTPTGWVGVADPRAGGGAAGY"
     gene            complement(1504..2229)
                     /locus_tag="VFU75_05370"
     CDS             complement(1504..2229)
                     /locus_tag="VFU75_05370"
                     /inference="COORDINATES: protein motif:HMM:NF024879.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HEU5466298.1"
                     /translation="MNVSIFRLAALLSSAAMIAACAKGDQANQAGTDSSATTAAAPAT
                     PMSGNDKPIPANAKPASKPAPARPAGPSSYTASAGSFLDVAVNDTISTKTAKVGDAFT
                     GTVVANVSDGRGATVIPQGAQVHGTVTDVQTSQLTLSVSSVTIRGKDYGLAAKIDSLE
                     TVKQSKGIGGNDVAKVGIGAAAGALLGRVIGGNTKGAVIGGVVGAAAGTGVAAKTHAS
                     YVALPKGAHINLTLTAALVVPAK"
     gene            complement(2256..2984)
                     /locus_tag="VFU75_05375"
     CDS             complement(2256..2984)
                     /locus_tag="VFU75_05375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEU5466299.1"
                     /translation="MEGSNGVSLLIAGLAGLLSFLSPCVLPLIPSYVGFITGMTAEET
                     TGRRRLVLFHALSFVLGFTFIFIALGASVSLVGGLLIHSQVWLGRIGGVLVLLLALYL
                     LGILRPAWLMRERRLELSTKPLGFVGSAVVGVTFGAAWTPCIGPILGAILTLAATAGS
                     TGKGTALLTVYAAGLAVPFLLTALLLERFLEWFQRFRRYLPWVERTSGVLLLLLGVLL
                     VTDRFTLLAGYLQGLTPEFLKSRL"
     gene            complement(2971..3873)
                     /gene="speB"
                     /locus_tag="VFU75_05380"
     CDS             complement(2971..3873)
                     /gene="speB"
                     /locus_tag="VFU75_05380"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HEU5466300.1"
                     /translation="MDKKLAELPWGAPQGFLGLPEPEVPLSKAGVVILPVPYEATVSY
                     MGGTRFGPRRIIEASRYIELYDHELDSEPYTVGVHTLPELMLSGAGPEEALEELREAF
                     DGLLAPNRFVIMLGGEHSLSAPPIQAHVAKVKGKKLSVLQLDAHGDLRAEYEGTPFSH
                     ACVMYRVHQDVNLVPVGIRAITTEERALMRKKKIPTVFGYELDPEERWIDRVLESLGP
                     DVYITIDVDYFDPAYVPATGTPEPGGGSWYPTLRLLDRVFKEKRVVGADVVELAPIPG
                     FVAPDFLVAKLVYKLIGFHQRGRK"
     gene            complement(3866..4468)
                     /locus_tag="VFU75_05385"
     CDS             complement(3866..4468)
                     /locus_tag="VFU75_05385"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF013979.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006596 - polyamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase, pyruvoyl-dependent"
                     /protein_id="HEU5466301.1"
                     /translation="MSAVEPIRSDEVTELIDSKMFFPTKAFLTKGVGRHRHKLTSFEM
                     ALRDAHLAAYNLVRVSSIFPPHCELMDREEGQSLLKPGQVVFAVIAESSTNEPSRLVA
                     ASIGLAMPADPSHHGYISEHHGYGQNEVAAGDYAEDLAASMLATVLDVPFDPEQAWDA
                     RREQWLMSGEIVRTLNVTSTAECGEDGRWTTVVSAVAFCG"
     gene            complement(4432..4896)
                     /locus_tag="VFU75_05390"
     CDS             complement(4432..4896)
                     /locus_tag="VFU75_05390"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEU5466302.1"
                     /translation="MKVDPAHLTDAFRRYLSDHTLPATNQRLAVARAVFEASDHLSVD
                     EVSRRVAAGGTAVGTATVYRTLDLLVQSGLARQHDFGEGFKRYEPTAPGERHEHCVCT
                     SCGAVTEFANDRLERMIGLLAEEVAFRPHHHRLEVYGLCRSCQQSNPFARTR"
     gene            complement(4886..5281)
                     /locus_tag="VFU75_05395"
     CDS             complement(4886..5281)
                     /locus_tag="VFU75_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466303.1"
                     /translation="MGTGPDAGSAGPLSPTPGQGPGWAKAAAAVAAQVPPAEIEAIHV
                     FRPIKREGREWGTAVITRRHPEGRLQVFTVKYMLIIRGKDRGQSKVVVEEVGLSPEEV
                     LRQVMQTTAERTGEAEPPVALGPKAWYEG"
     gene            complement(5242..6366)
                     /locus_tag="VFU75_05400"
     CDS             complement(5242..6366)
                     /locus_tag="VFU75_05400"
                     /inference="COORDINATES: protein motif:HMM:NF044507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466304.1"
                     /translation="MSRPRFQYRPPNFDAPPLSGAPEARFQPAPADGVLPDGFFSTSN
                     LPTYVKTGGAWRRPRLPRMDCVIVQHPTGDLVTTEPRKIKRGTLVAVGSAEDGSEGIF
                     VHTEGFLGGRHSANEFRFMQTEVSRERPVDYSVLAQLLGEEKARGGNIVWVIGPALVH
                     ARAREDMIWFIENGYAGALLGGNAVAVHDLEAAIFGTTLGMSSSGEGVEGGHALHMRA
                     INAIRSAGSIEAAVRSGQVKGGIMHALVTRKVPFVLAGSIRDDGPLPEVLSDTLAAQD
                     AMRDVTSKATFAIFVATALHAIAVGNMLPAFVDAATPDGVRPLTTICVDQTEFVVNKL
                     RDRGTHQAYGVVTNAQDFMHVLRFYVERWEQARTPAPLAR"
     gene            complement(6538..8760)
                     /gene="priA"
                     /locus_tag="VFU75_05405"
     CDS             complement(6538..8760)
                     /gene="priA"
                     /locus_tag="VFU75_05405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEU5466305.1"
                     /translation="MTSPRFAQVVLPLPVATPYTYAVPDALAPRVTPGVRVVVPVRNR
                     KAIGLVVAIDAAAPEAAAKPIADVADQDPVLSGPMLELAQWISRHYGAPLGLTVRAML
                     PGALWAVGRPAGPPDASVAVLRLERRLPSLLEREQAFARAPKRRVVYEAVEALGGSAP
                     VKHLVEQMGFSRSALDGLVKGGWARIDRLKETRDPFAGLTVAPPAELTADQRRVVEAV
                     VKGEGGKPSLIHGVTGSGKTLVYLEVLRHVVDQGRGAIVLVPEIALTPQTVARVRGVF
                     GDQVAVLHSGLSEGERADAWRALRRGERRVAVGPRSAVFAPVQRLGAIVIDEEHESSY
                     KQGSAPRYHARDVAAVRARLEGAQLILGSATPSLETLHLVATGRITSHSLPQRIGDRP
                     LPPVEVVDLRTAPRVRDSGGVPWSEVLDESVRRALARNEQVILLLNRRGFATYLQCPA
                     CGHVPECPNCSIALTVHKTPAGLKCHYCDHTESVPAACVRCGGEAVRNKGLGTQQLER
                     FLSSRYPAARIARMDLDTTSSKWAHHRILERVARKEVDILVGTQMIAKGLDFPDVTVV
                     GVVDADTALHLPDFRAAERTFQLIAQVAGRAGRGPAGGRVLVQTRAPEHHAIRAAARH
                     SVEAFAQIELPLRAAPNPVYPPHCGLARFVVGAGTETRAQESAEKLAGWLHRVNGEHL
                     EGALRVLGPAPCPIPRLQGRWRWHVLVKSDAPAPLGRLVRAWRGRTGVTVDRDPLSLL
                     "
     gene            complement(8757..>9580)
                     /locus_tag="VFU75_05410"
     CDS             complement(8757..>9580)
                     /locus_tag="VFU75_05410"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HEU5466306.1"
                     /translation="LEQHLPKDPFDLDAAEARFAQVVPKNGAARAALSGALHDLVGKR
                     LGVPLYKLWGLDPKKAPVSSFTIGIDTPEKTRAKVKEASGYSILKIKLGTDRDEEILK
                     TIRDATDKPLRVDANAGWTKERALKMLPMLKEYGVEFVEQPLPPDDLEGIAAVRKAGI
                     LPVVVDESCIVATDIPRVAQAADGINIKLAKCGSLREALRMIATARAHKMLVMVGCMI
                     ETSIGITAAAHFTPLVDAADLDGAALTANDPYRGAEISGAPIKIPDGPGLGVTRR"
BASE COUNT         1595 a         3296 c         3198 g         1491 t
ORIGIN      
        1 gcggcggatt catcgtcttc cgcgactcgg ccggcaatgc ccgcgcgctg gattaccggg
       61 agaccgcccc tgccgccgcc acgcacaaca tgtatctcga ttcggccggg aaccccaccg
      121 aagccagcat cacgggatac caggcgtccg gggtcccggg cagcgtggcg ggcatgtacg
      181 aggcttggaa gaagtacggc cacctgccct ggccgaagct gatcgctccc gcgatcgcac
      241 tggccgaagg tcacgtgatc gacgagaacc gttcccgcgc catcgagtac gatcgcgatc
      301 ggctcggcat gttcgccgcc agcgcggcga ccttcctggt gaacggccac gccccggcgg
      361 cgggcgccac cgtccggtac cccgagctgg cgaagaccct tcgcctgatc gccgactcgg
      421 gaccgagcgt gttctacagg ggccagatcg ccgaccagat cgtggcggag atgaagcgcg
      481 ggaatggcat catcaccaag gcggacctcg ccggctatca ggcgaaatgg cgcacgccca
      541 tcgaggtcac ctaccgcgga taccggatct attcgatgcc gcccgccagc tccggcggca
      601 tcacgatggc ggagatcttc aacatcctga gcggctacga ctcgctctcc gcgttcggga
      661 gtgcccgcta cgtgcacctc gaggcggagg ccatgcgccg ggctttcatc gaccggaatg
      721 aatggctggg cgatccggac ttcgtgaaga tgcccctcga ccgcctgctc tcggccgcgt
      781 acgccagcca gctgcggtcc cagatcctac gcgacaaggc cacaccgacg ccgcccttcc
      841 agacgaagcc ccccgagagc gggcagacca cccactactc ggtggtggac gatcacggca
      901 acgcggtctc ggtgacgacc acgctgaaca acagcttcgg cagcgcggtc acggtgacgg
      961 gcgccggttt cctgctcaac gacgaaatgg atgatttcgc gagcgctccg gggaagccga
     1021 acatgttcgg tctcgtgcag ggcgagcaga acgcgatcgt gcccggcaag cggatgctgt
     1081 ccgccatgac gccctcggtg gtactcgatc catccgggaa actcttcatg gtggtcggca
     1141 cgccgggcgg accgaccatc attaccagcg tataccaggt catcgcgaat gtgatcgacc
     1201 agcacatgtc cctgccggcg gcggtcgaag cgccccgcat tcaccagcag gcgctccccg
     1261 acgtcgtgtt ctacgagcgg ggcggattgt cccaggcggt cgtcgacagc ctcacggcga
     1321 tgggctacac catgaaggag cgccgtggct actccggtga cattgcggcg atcgagaaga
     1381 cgcccaccgg gtgggtcggg gtggcggatc cgcgggccgg tgggggggcg gcaggatact
     1441 gaggggctgg gggtcagggg ccaggggcca gggaaaacac cctgacccct gacccctgac
     1501 gccttacttc gccggcacca ccaacgccgc cgtaagcgtc aggttgatgt gcgcgccctt
     1561 ggggagcgcg acatacgacg cgtgagtctt ggccgcaacg ccggtacctg ccgccgcgcc
     1621 taccacccca ccgatcaccg cacccttggt gttaccaccg atgacgcgtc cgagcagcgc
     1681 accggcagcc gcgccgatgc cgaccttggc gacgtcattg ccaccgatcc ccttgctctg
     1741 cttcaccgtt tcgagcgagt cgatcttggc ggcgaggccg tagtccttgc cccgaatggt
     1801 cacgctgctc accgacaggg tgagctgact ggtctgcacg tcggtcacgg tgccgtgcac
     1861 ctgcgcgccc tgcgggatga cggtcgcgcc gcgcccatcc gacacgttgg cgacgacggt
     1921 accggtaaag gcatcgccga ccttggcggt cttcgtcgag atggtgtcat tcaccgcgac
     1981 gtcgaggaag gagcctgcgg aagcggtata gctcgacggg cctgcgggcc gcgcgggcgc
     2041 cggcttgctc gccggcttgg cattcgcagg gattggcttg tcgttgccgg acatcggcgt
     2101 ggctggtgcc gccgccgtgg tcgccgaaga atcggtgcca gcctggttcg cctgatcacc
     2161 cttggcacag gcggcgatca tcgcggcgct cgaaagcagc gcggcgaggc ggaagatcga
     2221 gacgttcata tgcacctcac gttgtgattg aagcatcaga gccggctttt caaaaactcc
     2281 ggcgtcagcc cttgcagata ccccgcaagg agggtgaatc ggtccgttac aagtagaacc
     2341 ccgagcaaaa gtagcagaac gcccgacgtt cgctcaaccc aggggaggta ccgccgaaag
     2401 cgctggaacc actcgaggaa ccgctcgagc aggagcgccg tcagcaggaa ggggacggcg
     2461 agaccggccg cgtacaccgt cagcagggcg gtgcccttcc ccgtgctccc ggccgtggct
     2521 gcgagcgtca ggatggcccc cagaatgggg ccgatgcagg gggtccaggc cgcgccgaac
     2581 gtcactccca ccaccgcgga tccgacgaag ccgaggggtt tggtactgag ctccagccgc
     2641 cgctcccgca tgagccacgc cgggcggagg atgcccaaca ggtagagcgc gaggaggagg
     2701 acgagcacgc cgccgatccg acccagccag acctgggagt ggatgaggag tccacccacg
     2761 agcgagaccg aggcgccgag ggcgatgaag atgaaggtga acccgaggac gaaggagagg
     2821 gcgtggaaca acaccagtcg cctgcgcccg gtggtctcct cggcggtcat tccggtgatg
     2881 aacccgacgt agctgggaat gagggggagg acgcaggggg acaggaaact cagcagcccc
     2941 gcgaggcccg cgatgaggag cgagactccg ttacttcctt ccacgctgat ggaatccgat
     3001 gagcttgtag acgagcttcg ccaccaggaa gtccggcgcg acgaacccgg ggatcggcgc
     3061 gagctccacg acgtcggcac caacgacccg cttttccttg aagacccgat ccagaagccg
     3121 cagcgtcgga taccaggacc cgccccccgg ctccggcgta ccggtggccg gcacgtacgc
     3181 ggggtcgaag tagtcgacgt cgatggtgat gtagacgtcc gggcccaggc tttccagcac
     3241 gcggtcgatc caccgctctt cggggtcgag ctcgtacccg aacacggtgg ggatcttctt
     3301 cttccgcatc agcgcgcgct cttcggtggt gatcgcgcgg atccccaccg gcacgagatt
     3361 cacgtcctgg tgcacccggt acatcacgca ggcatgcgag aacggcgttc cttcgtactc
     3421 ggctcgaaga tccccatgcg catcgagctg cagcacggag agcttcttcc ccttcacctt
     3481 cgccacgtgg gcctgaatgg gcggcgcgga cagcgaatgc tcgccgccaa gcatgatgac
     3541 gaaccggttg ggcgcgagga gcccgtcgaa ggcctcgcgg agctcttcca gggcctcctc
     3601 cggcccggca ccggacaaca tgagctccgg aagggtgtgc acgccgaccg tgtagggctc
     3661 ggagtccagc tcgtggtcgt acagctcgat gtagcgggag gcctcgatga tgcggcgggg
     3721 gccgaaccgg gtgccaccca tgtacgacac cgtggcctca tacggcaccg gcagaatgac
     3781 gacacccgcc ttgctcagcg ggacttcagg ctccggcaat cccaggaaac cctggggggc
     3841 accccagggc agctcggcga gcttcttatc cacagaaggc caccgcggac accacggtgg
     3901 tccaacgccc gtcctcaccg cactcggcgg tcgaagtgac gttcaacgtc cgcacgatct
     3961 cgcccgacat cagccactgc tcgcgccgcg catcccaggc ctgttcgggg tcgaacggca
     4021 cgtcgagcac ggtggcgagc atcgatgccg cgaggtcctc ggcgtagtcg ccggcggcga
     4081 cctcgttctg cccgtagccg tggtgctcgg agatgtaccc atggtgcgaa ggatcggcgg
     4141 gcatggcgag cccgatcgag gccgccacga gccggctcgg ctcgttggtg gacgactccg
     4201 cgatcaccgc gaagaccacc tgccccggct tgaggaggct ctgtccttct tcccgatcca
     4261 tcagctcaca gtgcggcgga aaaatcgagc tgacccgcac caggttgtag gcggccaggt
     4321 gcgcgtcgcg cagcgccatt tcaaaactgg tgagcttgtg ccgatggcgc cccacaccct
     4381 tggtgaggaa cgccttcgtg ggaaagaaca tcttcgagtc gatcaactcc gtcacctcgt
     4441 ccgagcgaat gggttcgact gctgacacga ccggcagagc ccgtacactt cgaggcgatg
     4501 gtggtggggc cggaacgcca cctcctccgc cagcaacccg atcatgcgct ccaggcggtc
     4561 gttcgcgaac tccgtaaccg cgccgcagga ggtgcagacg caatgctcgt ggcgctcgcc
     4621 cggtgccgtg ggctcgtagc gcttgaagcc ttctccgaaa tcgtgctgcc gggcgagtcc
     4681 actctgcacc agcaggtcca gcgtgcggta cacggtcgcc gtgcccaccg cggttccgcc
     4741 ggccgccacc cgccgggaca cttcgtccac cgacaggtga tcgctcgctt cgaagaccgc
     4801 gcgcgccacc gccagccgct ggttcgtcgc cggcaacgtg tggtcgctca ggtagcggcg
     4861 aaaggcgtcg gtcagatgcg ccgggtcaac cttcatacca cgctttcgga ccgagcgcca
     4921 ccggtggctc ggcctcgccg gtgcgctccg cggtggtctg catgacctgc cgcaagactt
     4981 cctcggggga gagaccgact tcctccacca ccaccttcga ctgaccccgg tccttcccgc
     5041 ggatgatgag catgtatttc accgtgaaaa cctgcaagcg cccttccgga tggcgccggg
     5101 tgatcacggc ggtgccccac tcccgtcctt cccgcttgat gggccggaag acgtgaatcg
     5161 cctcgatctc ggcgggcggc acctgcgccg ccaccgccgc cgccgccttg gcccagccgg
     5221 ggccctggcc cggcgtcggg ctcagcgggc cagcggagcc ggcgtccggg cctgttccca
     5281 tcgctccacg tagaaacgca ggacgtgcat gaagtcctgg gcgttggtga cgacgccgta
     5341 cgcttgatgc gtaccccggt cccgcagctt gttcacgacg aactcggtct ggtccacgca
     5401 gatcgtcgtg aggggcctga cgccgtcggg agtcgccgcg tccacgaacg cgggaagcat
     5461 gttgccgacg gcgatcgcgt gcagcgcggt cgcgacgaaa atcgcgaacg tcgccttgct
     5521 cgtgacgtcc cgcatcgcgt cctgcgcggc gagcgtgtcg ctcagcacct cggggagcgg
     5581 accgtcgtcc cggatcgagc cggccagcac gaagggcacc ttgcgggtga cgagcgcgtg
     5641 catgatgccg cccttcacct ggcccgagcg caccgcggct tcgatggacc cggccgagcg
     5701 gatggcgttg atcgcgcgca tgtggagcgc gtgtccgcct tcgactcctt cgccgctcga
     5761 cgacatcccc agcgtagtgc cgaagatcgc cgcctcgagg tcgtgtaccg ccacggcgtt
     5821 tccaccgagg agagcgcccg cgtacccatt ctcgatgaac cagatcatgt cttcacgcgc
     5881 gcgggcgtgg acgagggcgg ggccgatcac ccagacgatg ttgccgccgc gggccttctc
     5941 ttcgcccagc agctgcgcca gcaccgaata gtcgaccggt cgctcccggg aaacctcggt
     6001 ctgcatgaag cggaattcgt tcgcgctgtg ccggccgccg aggaacccct cggtgtggac
     6061 gaaaatcccc tcgctcccgt cctccgcgga cccgacggcg acgagtgtcc cgcgtttgat
     6121 cttgcgtggc tccgtggtca ccagatcgcc ggtcggatgc tgaacgatga cgcagtccat
     6181 gcggggcagg cggggacgac gccacgcgcc ccccgtcttg acgtaggtgg gcaggttcga
     6241 cgtcgaaaaa aagccgtcgg ggaggacccc gtcggccggt gcgggctgaa agcgcgcctc
     6301 aggtgccccc gagaggggcg gcgcgtcgaa gttgggcggt cggtactgga atcttgggcg
     6361 gctcacagga cggtcaaaat acccgacgcg aaacgcccaa cgcaacgccc gggccgtcga
     6421 tcccaggctg gaagcctggg cccgacacta cacagcgctt caagcgcgac gcccggtcgg
     6481 gcccaccctt gagggtgggg acgaccgggg gcggtaacag cgcctctccc ctagcgctca
     6541 cagcaaagaa agcgggtcgc ggtcgaccgt gactccagtt cgcccgcgcc aggccctcac
     6601 gagcctcccc aaaggcgccg gagcgtcgct cttcacgagc acatgccagc gccagcgccc
     6661 ctgcaggcgc ggaatgggac agggcgccgg gcccagcaca cgcaacgccc cttccaggtg
     6721 ctccccgttc acgcggtgca gccatccggc gagcttttcc gccgactcct gggccctggt
     6781 ttccgttcct gcacccacca cgaatcgcgc cagaccgcaa tggggcggat acaccgggtt
     6841 gggcgcggcc cggaggggga gctcgatctg cgcgaacgcc tcgaccgaat gcctggcggc
     6901 ggcgcgtatg gcgtggtgtt cgggcgcgcg ggtctgcacc agtacccgcc ccccggcggg
     6961 gccgcgcccc gccctcccgg ccacctgcgc gatgagctgg aaggtccgct ccgcggcgcg
     7021 gaaatccggg aggtgcaacg ccgtgtcggc atccaccacg cccaccacgg tgacgtccgg
     7081 aaagtcgagc cccttcgcga tcatctgcgt gccgaccaga atgtccactt ccttcctggc
     7141 cacgcgctcg agaatccggt gatgcgccca cttggagctg gtcgtgtcga ggtccatccg
     7201 cgcgatgcgc gcggcgggat accgcgaact gagaaagcgc tccagctgct gcgtcccaag
     7261 ccccttgttt ctgaccgcct ctcccccgca ccggacgcag gcggctggaa cggactccgt
     7321 gtggtcgcag tagtggcact tcaatccggc cggcgtcttg tggacggtca gcgcgatgga
     7381 gcagttggga cattccggca cgtggccgca ggccggacac tggaggtacg tcgcgaaccc
     7441 acggcgattg aggaggagaa tcacctgctc attccgcgcc agggcccggc ggacgctttc
     7501 gtccagcact tcgctccagg gaacgccgcc ggaatcgcgc acgcgcggag cggttcgcag
     7561 atccaccacc tcgacgggcg gcagggggcg gtcaccgatg cgctgcggca gtgagtgcga
     7621 cgtgattcgt ccggtcgcta cgaggtggag cgtttcgagt gacggcgtcg cgctgccgag
     7681 gatgagctgt gcgccttcga gacgggcgcg cacggcggcg acgtcgcgcg cgtggtaccg
     7741 cggcgccgac ccctgcttgt agctgctttc gtgctcctcg tcgatcacga tggcccccag
     7801 ccgctggacc ggcgcaaaca ccgccgaccg gggcccgacg gccacgcgcc gttcaccacg
     7861 gcgcagggcc ctccacgcgt cggcccgttc tccttccgag agcccagagt gcagcaccgc
     7921 cacctggtcc ccgaatacgc cgcgcacgcg cgcgaccgtt tgcggagtca gtgcgatttc
     7981 cgggacgagc acgatcgcac cccggccctg gtccacgacg tgacgcagga cctcgaggta
     8041 cacgagcgtc ttccccgatc cggtcactcc gtggatgagc gagggcttgc ctccttctcc
     8101 tttcacgacg gcctcaacca cgcggcgctg gtccgcggtc agctcggcgg gcggcgcgac
     8161 ggtgagtccg gcgaacggat cgcgggtctc tttcagccgg tcgatgcgcg cccatccgcc
     8221 cttcaccaat ccgtcgagcg ccgagcggga gaatcccatc tgttcgacga gatgctttac
     8281 cggtgccgag ccgccgaggg cttccaccgc ttcgtagacg acgcgacgct tgggagcgcg
     8341 agcgaacgcc tgctcacgct cgaggaggct gggcagacgg cgttcgaggc gcaggacggc
     8401 gaccgacgcg tccgggggac ccgcgggccg tcccaccgcc cacagtgccc cggggagcat
     8461 ggcccgcacc gtcagcccga ggggagcgcc gtagtgtcgg gagatccact gggccagctc
     8521 gagcatcgga cctgaaagaa ccggatcctg atcggcgaca tccgcgatcg gcttggcggc
     8581 cgcctccggc gcggctgcgt cgatggcgac gacgagcccg atcgccttgc ggttgcggac
     8641 cggcacgacg acccgaaccc cgggcgtgac ccgcggtgcc agggcgtcgg ggacggcgta
     8701 ggtgtaggga gttgcgaccg gaagcgggag gacgacctgc gcgaaccggg gggaggtcac
     8761 cgcctggtga cgcccagtcc ggggccgtcg gggatcttga tcggcgcgcc gctgatttcc
     8821 gctccgcggt agggatcgtt ggcggtgagc gcggcgccat ccaggtcggc ggcatccacg
     8881 agcggcgtaa agtgagcggc cgccgtgatg ccgatcgacg tttcgatcat gcaccccacc
     8941 atcaccagca tcttgtgggc gcgcgcggtc gcgatcatcc gcagggcctc tctcagcgac
     9001 ccgcacttcg cgagcttgat gttgatgccg tcggccgcct gcgctacgcg agggatgtcg
     9061 gtggcgacaa tgcagctctc atccaccacc accggaagaa tcccggcttt gcgcaccgcg
     9121 gcgatgccct cgaggtcgtc tggcggcaaa ggctgctcca cgaactccac gccgtattcc
     9181 ttgagcatcg ggagcatctt gagagcgcgt tccttggtcc agccggcgtt cgcatccacg
     9241 cggagcggct tgtccgtggc gtcacggatg gtcttgagaa tctcctcgtc ccgatcggtg
     9301 cccagcttga tcttcaagat cgagtacccg gaggcttcct ttaccttggc cctggtcttt
     9361 tcgggcgtgt cgatgccgat ggtaaaggaa gagacgggtg ccttcttcgg gtcgagtccc
     9421 cacagcttgt atagggggac gccgaggcgt ttccccacca ggtcatggag ggcaccggac
     9481 agagcggccc gcgcggcgcc gttcttcggc accacctggg cgaaccgtgc ctccgccgcg
     9541 tccagatcga acggatcctt cggaaggtgc tgctccagaa
//
LOCUS       DASUBP010000146         3347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_903281, whole genome shotgun sequence.
ACCESSION   DASUBP010000146 DASUBP010000000
VERSION     DASUBP010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3347
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903281"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            113..586
                     /gene="ruvC"
                     /locus_tag="VFU75_05415"
     CDS             113..586
                     /gene="ruvC"
                     /locus_tag="VFU75_05415"
                     /inference="COORDINATES: protein motif:HMM:NF000711.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HEU5466307.1"
                     /translation="MRVLGVDPGTAVTGYGIVERNGGPGFGHLIECGVIRLPRSGRLE
                     VRLAELYAGIAELIARHHPTHLALEDAFYHKNVRTTLVLGHARGVVLLAAAQRGVEIA
                     EYSPARIKKTVVGAGGAPKAQVAAMVAKLLRLQTAPKPSDAADGVAVALTHIIRS"
     gene            583..1197
                     /gene="ruvA"
                     /locus_tag="VFU75_05420"
     CDS             583..1197
                     /gene="ruvA"
                     /locus_tag="VFU75_05420"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEU5466308.1"
                     /translation="MISRLTGRIAGMHGDRVTVDTAAGVGYEVVVPASVLERLGPPGS
                     EVTLATELVVRDDAWTLYGFGAESERRFFQRLMTVSGVGPKLALALVSALGLDRGARA
                     IRGRDAATLASVSGVGKKTAERLVLELADKVGDFVEGGAPGAAPAPLAGGARAARQAL
                     ERLGYSPAESDRAVRRALTESGAPVDADGTEPLVRRALQLLTHG"
     gene            1194..1367
                     /locus_tag="VFU75_05425"
     CDS             1194..1367
                     /locus_tag="VFU75_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466309.1"
                     /translation="MTMKTAEWICTRCGSTNRRLVEDGERHATDACVTCHTRHDIEAE
                     ARPVRWQARPHGT"
     gene            1364..2395
                     /gene="ruvB"
                     /locus_tag="VFU75_05430"
     CDS             1364..2395
                     /gene="ruvB"
                     /locus_tag="VFU75_05430"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEU5466310.1"
                     /translation="MSRPEVTTPEALPDERLSDAALRPSRLDEFVGQPKVKESLQIAI
                     DAARRRHEPLDHTLFFGPPGLGKTTLALLMAKEMGVEIRITSGPVLERPGDLVGMLTN
                     LHAGDILFIDEIHRLRPALEEFLYPAMEEFRVDVRIADGPHAQSLPMALERFTLIGAT
                     TRFGLLTPPMRARFGLVERLSYYPPDDLTHILSRSAKILEVPADAAGVAEIARRSRGT
                     PRVANRLLKRVRDFAQVRADGKITAPVAKDALARLDVDEFGLDDMDARLLKTIIEQFS
                     GGPVGLTTIAAAVGEDPGTLEEVYEPFLMQQGFLARTPRGRCATAAAYRHFGFPPPAT
                     SEQPSIFDG"
     gene            2392..>3347
                     /gene="queA"
                     /locus_tag="VFU75_05435"
     CDS             2392..>3347
                     /gene="queA"
                     /locus_tag="VFU75_05435"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEU5466311.1"
                     /translation="MTRSLHTGDFDFDLPPDRIAQTPLADRSASRLLVLDRAQGSVAH
                     RHFRDLPELVGPADVVVLNASRVIPARLRGKRETLKDSLPVAGNAAEILLVRDLGDGT
                     WEAMGHPGGKLKPGRRVTFGDDAAVEILAVLGGGLRRVKFVGTLDARGTVAKYGEIPL
                     PPYIRRAPDAADRERYQTVYAAHDGSVAAPTAGLHFTPEVLVAIRDRGASIVTLDLHV
                     GPGTFKPVEVDDPAAHVMHLEAYTVSREAAEEINDRRAGGGRIWAVGTTVVRTLETVA
                     DDSGRVHAGAGETRAFITPPYRFKAVDRMVTNFHLPRSTLLML"
BASE COUNT          514 a         1180 c         1153 g          500 t
ORIGIN      
        1 gccaagcgcc tcctggcgct ggtggaggca ctcgaggagt cagacgacgt agccaaggtg
       61 tcgtccaact tcgatatcga cgccgaggcg ctcgcggcga tcgacggatg acgtgagggt
      121 cctgggtgtc gacccgggga cggcggtcac cgggtacggg atcgtcgagc ggaacggtgg
      181 cccgggattc ggacacctga tcgagtgcgg cgtgattcgc cttccccgct ccggcaggct
      241 cgaagtcagg ctcgcggagc tctacgccgg catcgcggag ctgatcgcca ggcaccaccc
      301 cacgcacctc gctctggaag acgctttcta tcataagaac gtccgcacca ccctggttct
      361 cggacatgcc cggggtgtgg tgctgctcgc ggccgcgcaa cggggggtcg aaatcgccga
      421 gtactctccc gcccggatca agaagaccgt cgtcggcgcc ggaggcgcac ccaaggcgca
      481 ggtggcggcg atggtcgcca agctgctgcg tctccagacc gcgccgaaac cctccgatgc
      541 ggccgacggc gtcgccgtcg ccctcaccca catcatccgc tcatgatctc ccggctcacc
      601 ggccggatcg ccggcatgca cggagaccgc gtcaccgtcg acacggcggc gggcgtgggc
      661 tacgaggtgg tggtgccggc gagcgtgctc gagcggctgg ggcctccggg gagcgaggtg
      721 acgttggcga ccgagctggt ggttcgtgac gacgcctgga cgctctatgg atttggcgcg
      781 gagtcggagc ggcggttctt ccagcgcctg atgacggtgt ccggggtcgg acccaagctc
      841 gccttggcgc tggtgtcggc cctggggctc gaccggggag cccgagccat ccgcggccgg
      901 gatgcggcga cgctcgcgtc ggtctcgggc gtcgggaaga agaccgcgga acggctggtg
      961 cttgaactgg ccgacaaggt gggcgacttt gtggagggtg gcgctccggg ggccgcgccc
     1021 gcgcctctgg ccggcggcgc gcgggccgcg cggcaggcgc tcgaacgact gggctactcg
     1081 cccgcggagt cggatcgcgc ggtgcgccgt gccctcacgg aatcgggggc accggtcgac
     1141 gccgacggga ccgagccgct cgtgcggcgg gcactgcaac tcctcaccca cggatgacga
     1201 tgaagaccgc cgagtggatc tgtacgcgct gtggcagcac caaccgccgc ctggtcgagg
     1261 acggtgaacg gcacgcgacc gacgcctgtg tgacgtgcca tacccgtcac gatatcgagg
     1321 ccgaagcgcg cccggtccgg tggcaggcac ggcctcacgg cacgtgagcc ggcccgaagt
     1381 caccacccct gaagcccttc cggacgagcg gctgtcggac gccgcactcc ggccctcccg
     1441 gctcgacgag ttcgtcggac aacccaaggt caaggagtcg ctgcagatcg cgatcgacgc
     1501 cgcgcgccgg cgccacgagc cgctcgacca caccctgttc ttcggcccac ccggactcgg
     1561 caagacgacg ctcgcactgt tgatggcgaa ggagatgggc gtcgagatcc gcattacctc
     1621 ggggcccgtg ctcgagcgcc cgggagatct ggtggggatg ctcaccaacc tgcacgccgg
     1681 cgacatcctc ttcatcgatg agatccaccg cctgcgcccc gccctggagg agtttctcta
     1741 tcccgccatg gaggaattcc gcgtggacgt ccgcatcgcg gacgggccgc acgcgcagag
     1801 cctgcccatg gccctcgaac gcttcacgct gatcggcgcg acgactcgct tcggcctgct
     1861 cacgccaccg atgcgggccc ggttcggcct ggtcgaacgg ctgtcctact atccgcccga
     1921 tgacctgacc cacatcctct cgcgctccgc gaagattctc gaggttccgg cggacgcggc
     1981 cggcgtcgcg gaaatcgcgc gacggagccg cggcacgccg cgggtcgcga atcgcctgct
     2041 gaagcgggtg cgggattttg cgcaggtcag ggccgacggc aagatcacgg caccggtggc
     2101 gaaggacgca ctggcgcggc tcgacgtcga tgagttcggc ctcgacgaca tggatgcgcg
     2161 gctcctgaag accatcatcg agcagttctc gggtggcccg gtgggactca ccaccatcgc
     2221 cgccgcggtc ggggaagatc ccggcacgct ggaagaggtc tacgagccgt tcctcatgca
     2281 gcagggattc ctggcccgca ccccccgcgg ccgctgcgcc accgcggccg cgtaccgtca
     2341 cttcggattc ccgcctcccg cgacgtccga gcagccgagc atcttcgacg ggtgacgaga
     2401 tcccttcaca ccggcgactt cgacttcgac ctgccgccgg accgcatcgc ccagaccccg
     2461 ctcgccgacc gctccgcctc ccgtcttctg gtgctcgatc gcgcgcaggg gtcggtcgcg
     2521 catcggcact ttcgcgactt gccggagctc gtcggaccgg cggacgtcgt agtcctcaat
     2581 gcctcccggg tcataccagc ccggttgcgc gggaaacgag aaaccctgaa ggactccctg
     2641 ccggtcgcgg gaaacgcggc cgagatcctc ctggtcaggg acctgggcga cggcacctgg
     2701 gaagcgatgg gacaccccgg gggcaaactg aagcctgggc gtcgcgtcac cttcggcgac
     2761 gacgccgcag tcgagatcct cgccgtgctg ggtggtggct tgcggcgggt gaagttcgtc
     2821 gggaccctcg acgcgcgcgg caccgtcgcg aagtacggcg aaatccccct gcccccatac
     2881 atcaggcggg cccctgacgc cgccgaccgg gagcgctacc agacggtcta tgccgctcac
     2941 gacggcagtg tcgccgcgcc aacggccggc ctgcacttca cgcccgaagt cctcgtcgcg
     3001 atccgcgacc ggggtgcatc gatcgtcacc ctcgacctcc acgtcggtcc gggaacgttc
     3061 aagcccgtcg aagtggacga tcccgccgcc cacgtgatgc acctcgaggc ttacacggtg
     3121 tcgcgggagg ccgcggagga gatcaacgac cggcgcgccg ggggagggcg aatctgggcc
     3181 gtgggcacga cggtcgttcg gacactcgaa acggtggccg atgactcggg tcgcgtccac
     3241 gccggggccg gtgagacgcg ggccttcatt acgcccccct accgcttcaa ggcggtggac
     3301 cggatggtca ccaacttcca tcttccccgc tccacgctgc tcatgct
//
LOCUS       DASUBP010000147         2377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1107744, whole genome shotgun sequence.
ACCESSION   DASUBP010000147 DASUBP010000000
VERSION     DASUBP010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1107744"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(238..1092)
                     /locus_tag="VFU75_05440"
     CDS             complement(238..1092)
                     /locus_tag="VFU75_05440"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU5466312.1"
                     /translation="MTEAPVDGSAIRDSRSALRVVAVFGGGGVKALAHIGAWKALQEK
                     GLAPTHIVGTSMGAVVGAALAAGMTGDEFLARLKGLTRPEVAPTDVLSFFKGIFAKSL
                     LKPAVWQETLSRLIPVSRFPDFRIPLTVCATDMDSGDLVLFGDRGKDVPVNLALAASC
                     ALPLYYQPVEIEGRRYADGGLRAVLPVHVARGIAADLIVAISVGPGFDEGPSKAKLPG
                     LLKAHGDAERIMMAAQTERDLEDWPADATKLMVVRAVHERDATFAMDQADRFVRQGYD
                     ATKQALGG"
     gene            complement(1129..1614)
                     /locus_tag="VFU75_05445"
     CDS             complement(1129..1614)
                     /locus_tag="VFU75_05445"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEU5466313.1"
                     /translation="MKPRHPRESETVMSQLMEPHHANIMGNVFGGVILSLADHVAAVA
                     AIRHSRHQCVTVSVDKVDFREPIRVGELVTAMSRVNFAGRTSMEIGVKIMAEVITTGE
                     RRHTNSCYLTYVALDDKGHPTEVPPIAPETPDEKRRYERAMQRRANRVTENRFNQGRG
                     E"
     gene            complement(1616..>2377)
                     /locus_tag="VFU75_05450"
     CDS             complement(1616..>2377)
                     /locus_tag="VFU75_05450"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HEU5466314.1"
                     /translation="LRDEVVVRRKFKKGIVALSGGVDSALTAFLAVQALGKENVIGVR
                     LPYRTSSPESLDHARLIIDQLGIPCETIDISLAVDGYLSQVKDAGPGRSGNVMARERM
                     IALFDLSERHKALPLGTGNKSERLLGYFTWHADDSPPVNPLGDLFKTQVWALARHVGV
                     PEVIVSKPATADLVAGQTDEGDFGISYPKADRILYELISGRKPQEVEALGFTAAEVER
                     VRSRLDATHWKRRLPTVAMLSQTAIGEFYLRPVDY"
BASE COUNT          399 a          781 c          757 g          440 t
ORIGIN      
        1 acgatgtcgc ggtcggatat cgtgccctga tagcggcggt ctttggggtt atcgacgacg
       61 ggcacaaccg aaatgttctt gatcctcatc aacgccgcga tctcggcgca cgttgcgccg
      121 gcggccgcga ccacgggatc cgcgatcatt acttcagatg ctttcatctg gttttaacta
      181 actcctatct cctgtaaacc gctaggcgcc tgagcgcctg ggcgcctgtc tggccgacta
      241 gccgcccagt gcctgcttcg tagcgtcgta gccctgtcgc acaaagcggt ctgcctggtc
      301 catggcgaag gtggcatccc gttcatgcac cgccctcacg accatgagct tcgttgcgtc
      361 tgccggccaa tcctcgagat cgcgctccgt ctgcgccgcc atcatgatgc gctcggcatc
      421 gccatgagcc ttgagtaggc ccggcagttt ggcctttgaa ggtccttcat cgaatcccgg
      481 tccgacactg atcgccacga tcaggtcggc ggcaatcccc cgggccacgt gcaccggcag
      541 cacggcccgc agaccgccat ccgcgtaccg ccggccctcg atctcgacgg gctggtagta
      601 caggggcagc gcgcaggaag cggcgagtgc gaggttgacc gggacgtcct ttccccggtc
      661 cccgaaaagg accaggtcac ccgagtccat gtcggtggcg cacaccgtca acgggatccg
      721 aaaatccggg aaacgggaaa cgggaatcag acgggaaagg gtttcctgcc atacggcggg
      781 cttcaacagg ctcttggcga agatcccctt gaaaaagctc aagacgtctg tgggtgcgac
      841 ctcggggcga gtgagtccct tcaaccgggc gaggaattca tcgcccgtca tgccggccgc
      901 gagcgccgcg ccgacgaccg cgcccatcga cgtgccgacg atgtgcgtgg gggcgaggcc
      961 cttctcctgg agtgctttcc aggcgccgat gtgcgcgagg gctttgacgc ccccgccccc
     1021 gaagacagcc accacccgta acgcggaacg cgaatcgcgg atcgcggaac cgtccaccgg
     1081 agcttccgtc accggttccg cattccccct tccgacttcc gcgtcgtctt actcaccgcg
     1141 cccctgattg aaccggttct ccgtgacgcg gttggcccgg cgctgcatgg cgcgctcgta
     1201 gcggcgtttc tcgtcgggag tttccggcgc gatgggcggc acttcggtgg gatggccttt
     1261 gtcgtcgaga gcgacgtagg tgagatagca cgaattcgta tgtcgccgct ccccggtggt
     1321 gatgacttcg gccatgatct tcacgccgat ttccatggag gtcctgcccg cgaagttgac
     1381 gcgcgacatc gcggtcacca gctcccccac cctgatgggt tcccggaagt cgaccttgtc
     1441 cacggatacc gtcacgcact gatggcgcga gtggcgaatc gccgcgacgg ccgcgacgtg
     1501 atcggcgagc gagagaatca cgccgccgaa cacgttcccc atgatgttcg catggtgcgg
     1561 ctccatcagc tgcgacatca cggtctcgga ttccctgggg tgacgtggct tcatgtcagt
     1621 agtccaccgg tcggaggtag aattcgccga tcgccgtctg ggacagcatc gcgaccgtgg
     1681 ggagccgccg cttccaatgt gtagcgtcga gccggctccg cacccgctcc acttctgcgg
     1741 ccgtgaaacc gagggcttcg acctcttgag gcttccggcc tgaaatcagc tcgtagagaa
     1801 tccggtcggc cttcggatac gagatcccaa aatccccctc atcggtctgt cccgccacca
     1861 ggtccgcggt cgcgggcttg gacacgatca cctccgggac ccccacgtgc cgggcgagcg
     1921 cccagacttg cgtcttgaag agatcaccca gggggttcac gggcggtgag tcgtcggcgt
     1981 gccaggtgaa gtagccgagc aggcgttcgg acttgttgcc ggtgccgagg gggagggctt
     2041 tgtggcgctc cgagagatcg aagagcgcga tcatgcgctc gcgggccatg acgtttcccg
     2101 agcgtccggg ccccgcgtcc ttcacctggg agagatagcc gtcgaccgcc agggagatgt
     2161 cgatcgtctc gcacggaatg ccgagttgat cgatgatgag acgcgcgtgg tcgaggctct
     2221 cgggactcga ggtgcgatag gggagccgca cgccgatcac gttctccttg cccagcgcct
     2281 gcacggcgag gaacgcggtg agggcggagt cgacgccacc ggagagcgcg acgatgccct
     2341 tcttgaattt ccgccgcacc acgacttcgt cccggag
//
LOCUS       DASUBP010000148        10645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1325825, whole genome shotgun sequence.
ACCESSION   DASUBP010000148 DASUBP010000000
VERSION     DASUBP010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 10645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10645
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1325825"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(365..826)
                     /locus_tag="VFU75_05455"
     CDS             complement(365..826)
                     /locus_tag="VFU75_05455"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU5466315.1"
                     /translation="MSNTGKNGKLSYAYELYIAAPAAKVWARLFERAVPLPFMFETKL
                     VGTLRKDTPYSYVGDGEFSVVDGRVLEVQPGLKMVMTWSAHWGEAENNDRPSRVTYEL
                     TTIGPKVTKLRVVHDDFDGETATYVASTASWPRMLSSLKSGVETGTAMAVA"
     gene            complement(859..1794)
                     /locus_tag="VFU75_05460"
     CDS             complement(859..1794)
                     /locus_tag="VFU75_05460"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU5466316.1"
                     /translation="MTARLSSARAIADLTSGMVFASVEIAVPPERVFRALTDPRELVK
                     WWGAPEVYRTEEWKVDLRVGGHWEVRGHEADGKAFVAQGQYVVIEPPHRLTLTWEPSW
                     LPGLKTTLSYRLEAVAGGTRLTLRHEGFLGQGDAAQSHTRGWEQVLGWLAKHLNAEAQ
                     PRPSVAARYFNPLRVATYLLVFYTFGHTVGALFNTPSFSPQADAVLASMRTVHFPVQG
                     VERTWFNFWFGFGLIDSVFFIASAAIAWFLGGRSPEDRRSLRFITWTLFLGYAASIVI
                     ILQDFFMGPLLFSTLITICFGIECWKVTRSSPARG"
     gene            complement(1791..2111)
                     /locus_tag="VFU75_05465"
     CDS             complement(1791..2111)
                     /locus_tag="VFU75_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5466317.1"
                     /translation="MPRALAHADVFYAVADPTRRAILDRLRAAPAPVNALADDFRVSR
                     PAVSKHLSVLRRARLVSETRMGRERIYKLEPDSLRQIAGWIEGYRVYWMTNLNKLKRH
                     LEKS"
     gene            complement(2185..3408)
                     /locus_tag="VFU75_05470"
     CDS             complement(2185..3408)
                     /locus_tag="VFU75_05470"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxide hydrolase"
                     /protein_id="HEU5466318.1"
                     /translation="MSGVRWKLTEMLAGAAGGHLETAEDREGVTRLTIRVPEAALRDL
                     RSRLARAMWPDPVRAEPWCDGTDRDFLQRLVDHWLHSFNWRTQERLINEWPHYRAVVG
                     GTGIHFVHVRGRHPNCIPLLLCHGWPSSFLEYRRLIPLLDDVFDLIIPSLPGFAFSDI
                     PSSSGMHVGRIATLYDELMTKVLGYQRYGAHGSDIGASVVSLIGLETPEHLLGVHLPY
                     VTGSAVKRELNHGAPPLTAAEREFLREAGEWNDVEGAYAHLHRTKPQTLAYALHDSPV
                     GLASWLVEKYRAWSDCHGDVTSRFSLDELLTTITLYWVTGTIGSSIRLYYEGRLHPRQ
                     LRPGERVEVPCGVSLFPGDIAHPPREWAERAYRVTRWTEMPCGGHFPAHEEPKLLAEE
                     LRAFFLPLDDREATP"
     gene            complement(3405..4193)
                     /locus_tag="VFU75_05475"
     CDS             complement(3405..4193)
                     /locus_tag="VFU75_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5466319.1"
                     /translation="MYRMVLAVATGLAGSIGLAAQQPAHLVSDSYRTEFELNATRIVA
                     AADDMPANRYAFRPTTAQMSFAEVVVHVLEGNDDIGAVVGDTTAPARPSLTANDGKAA
                     LVARLKDTFEFCRRALARINDSRLADPLPDYGTRAQALFNTINHWSDHYSQMAIYLRL
                     SGVLPPTARAARPAAPPPRADSTIHAGRTASPAPVTMHAHIWILGGEARDPDGSYHRS
                     VHDYRPQNGTFLALKESSADVVYGIVDRRRVVQRKHPLVHTDVQ"
     gene            4673..5011
                     /locus_tag="VFU75_05480"
     CDS             4673..5011
                     /locus_tag="VFU75_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466320.1"
                     /translation="MTTSMTDEMMRVQIANARAYTLVILHPTAKRQDPGADQVVWEHG
                     RRNLELRRDRRLSIVCPVRDGSNVCGIYVFPGTPEETGVIMDDDPGVKAGVFTYEVHA
                     CRGFPGDHLP"
     gene            5150..7339
                     /locus_tag="VFU75_05485"
     CDS             5150..7339
                     /locus_tag="VFU75_05485"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroloquinoline quinone-dependent
                     dehydrogenase"
                     /protein_id="HEU5466321.1"
                     /translation="MRLALLLLLAVSTPAVAQWPVYGGDAGGSHFSQLHQVDRSNVPT
                     LRMAWIARTGDYLTDRGRFEATPILVDGTLYVSTPLGSVLAFDPATGAELWKFDGPVR
                     FGHNDYGDFANRGVSAWAGGSRGSTCAHRLYVATVNARLLALDARTGHLCEGFGAHGT
                     VDLTQGLRHRPAYDWEYGVTSPPTVVGDLVIVGSAVADNHRTDAPEGVVRAFDVRTGA
                     LRWTFNPLPGLPNTGAANAWSIFSADTARDLVFVPIGSASPDFYGGARPGDDRYANSV
                     VALRASTGALVWAFQVVHHDLWDDDVPAQPTLFTLHRAGRSIPAVAVATKMGHLFILD
                     RRTGTPLFPVEEKPVPASDVPGETASPTQPFPPPAFRLAPESLSADQAFGANDSVRAV
                     CRARIAGLRFQGVFTPPSLQGTIIWPGNIGGMNWSGVSVDEARGILIAPTNRLAMVVR
                     LIPRDSLHAASMAQPDLEYGSQTGTPYASSRGLLGFCTPPPWGALTAVDLSAGAVKWQ
                     VPLGSMPQMAGVPGASGSIQLGGALITAGGLVFIAGTFDQHLRAFDIETGKELWSTGL
                     PAGAHALPMTYEAGGRQYVVIAAGGHDHLHTTMGDYVIAYALPGRGAPVPDTSAGNWT
                     GEWSGEMHIGDAKFGMQVRLADSGATAQLDSLQITAPVRMERSGRGVALSFPIYYAGK
                     AKCTGDFRVELELWNQGALLEGTGTFDGACASHGHETAAFVFHRRTQ"
     gene            7349..8419
                     /locus_tag="VFU75_05490"
     CDS             7349..8419
                     /locus_tag="VFU75_05490"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU5466322.1"
                     /translation="MTSRLRAGALWVGSFTALGLLMFAHFYLDVLARGHSEPVGIKLV
                     EELTASMGAGVVLLLPLAVLRRLRTAGWSGVRLVVVHAGLLPVFSVLHTSWNWATRSA
                     LFPLLGMGAYDYGRMPVRYPMEFPTDVIVYTFVITLMTVFIRYREARDREVRLAQVEF
                     ELSRVRLEALEGQLRPHFLFNALNTISSVMYEDVALADTLLTRLADLLRHTLQHAVGT
                     EVALRDELATLELYLAIMRARFAERLTVDVDIDPLALDARVPPLVLQPLVENAIKHGD
                     PGPGARANVFITGRRENGRLVLRVEDNGPGLPAAVTPHVGLGATARRLAALYGEGGGV
                     AVENRAGGGARASLWVPYRVET"
     gene            8416..9165
                     /locus_tag="VFU75_05495"
     CDS             8416..9165
                     /locus_tag="VFU75_05495"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEU5466323.1"
                     /translation="MNDGLRVLIVDDERPARQKLRRILGGEPGVEAVFEAPDGLRAVE
                     LIRSEDPDVVFLDIEMPGVDGFQVVEALGAESRPHIVFVTAYDRYAVRAFEAYAVDYI
                     LKPYDADRLRTALTRARERRNGPTRVDEMVVAVRRERPEPLERLLVEEGGRAILLPVG
                     SVDRFESDRNYVVVHAGTGRYRLRATLDGLEERLDLRKFARISRSAIVNLEGIVELRP
                     WGHGDYVVELKGGVELRLSRRYRDRLKHFVP"
     gene            9430..10617
                     /locus_tag="VFU75_05500"
     CDS             9430..10617
                     /locus_tag="VFU75_05500"
                     /inference="COORDINATES: protein motif:HMM:NF033577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HEU5466324.1"
                     /translation="MPWKECHVMDERLRFVARLLEGEKMAPLCAAFGISRKTGYKIFE
                     RYQTCGLGAFTDRSRRPYRQANRLPAPIEATIVRLKREYPGWGAPKIREKLRQQALGI
                     HLPAISTVHAVLDRHGLVRRRRRRRGAGAGTPLSQPTEPNALWCADYKGEFQLGNRQY
                     CYPLTITDFASRYLLTCEALSTTQEPFAFTVFERTFQEFGLPQAIRTDNGVPFASSHA
                     LYGLSKLAVWWLRLGIRLERIAPGQPQQNGRHERMHLTLKQEATKPAEANVLRQQARF
                     DAFVTRYNEERPHQALGMLVPAAVYVRSPRVYRGLEELTYPYHDATITVTHCGRLCFG
                     GRKINVSRVLAGQNVGVLQVGEHVWLVTFMQYDLGYFDDETCRLEPIAYPFGSKVLPM
                     CSV"
BASE COUNT         1787 a         3444 c         3557 g         1857 t
ORIGIN      
        1 ttcatgcact tctgggcgaa tggggatgct acgaaggtga ccgaagggct tcgcgcggcg
       61 cttgatcaaa tgaacgtcaa gaaggctggt tgagccgggg taagtcttcg cgatcttcat
      121 gccgaatcaa ctggtcgtga aactgcctca ggaccgggtg caagcgatgt tgtcgtaagc
      181 cggggttcgc acagttcgag cgacggaaga acgggcagtt ggtgaagatc cccacgatgg
      241 tgtctgtcgt gacctccctc acgaacgtgg tgtgcaaagg ttcacgcgaa gccgggtccg
      301 cgtacggcgc aagtgcaccc gttatctgcc tgctggtgat ccggacgaag ctgatggtga
      361 gcggtcacgc caccgccatc gccgtgccgg tttccacccc ggacttgagg gaagacagca
      421 tccgcggcca cgacgccgtc gaggctacgt acgtcgcggt ttctccatcg aagtcgtcgt
      481 gcacgacgcg gagcttggtg actttcgggc cgatcgtcgt cagctcatag gtcacgcggg
      541 aagggcggtc gttgttttcg gcctcgcccc aatgcgccga ccaggtcatt accatcttca
      601 gccccggctg gacctccagg actctcccat caaccacgct gaactctcca tcacctacat
      661 acgagtacgg agtgtccttg cgcagtgtgc cgaccagctt cgtctcgaac atgaacggaa
      721 gcgggacggc ccgttcgaac agcctggccc agaccttcgc cgccggggcg gcgatgtaga
      781 gctcatacgc gtacgacagt tttccgttct tgccggtgtt gctcatgctg aaagactcct
      841 gcgcagagag gatgcccgtc agccccgggc cggggaggat cgagtcacct tccaacactc
      901 gataccgaag cagatggtga tcagggtcga gaagagcagt ggtcccatga agaaatcctg
      961 caggatgatc acgatgctcg cggcgtaacc gaggaacagg gtccaggtaa tgaaccgaag
     1021 ggaccggcgg tcctcaggcg accgtccacc cagaaaccag gcgatggcgg ccgacgcgat
     1081 gaagaagaca gagtcgatga ggccaaatcc gaaccagaag ttgaaccagg tccgttcgac
     1141 gccctgcacg gggaagtgaa ccgtccgcat cgacgccagc accgcgtcgg cttggggact
     1201 gaacgacggc gtgttgaaga gcgcccccac ggtgtgcccg aaggtgtaga acaccagcag
     1261 ataggtcgcc acgcgcaggg gattgaaata ccgggcggcg acgctcgggc ggggctgtgc
     1321 ctcggcgttc aagtgcttcg cgagccagcc caggacctgc tcccagccgc gggtgtgact
     1381 ttgggccgcg tccccctgac cgaggaagcc ctcgtggcgc agggtcagcc gggtgccgcc
     1441 ggccacggcc tcgaggcgat aggacagcgt cgtcttcagg cccggcaacc agctcggttc
     1501 ccacgtcaag gtcaggcggt ggggcggctc gatgacgacg tactggcctt gcgccacgaa
     1561 ggccttgccg tccgcttcgt gcccgcggac ctcccagtgt ccgccgacgc ggaggtcgac
     1621 cttccactcc tcggtccggt agacctcggg agctccccac cacttcacca gctctcgggg
     1681 atccgtcagc gcccgaaaga cacgctcggg tggcacggca atctcgacgc tagcaaagac
     1741 cataccgctg gtgagatcgg cgatcgcgcg cgcactcgac aggcgtgccg tcatgacttc
     1801 tccaagtgac gcttcagttt gttgaggttc gtcatccagt acacgcgata gccctcgatc
     1861 catccggcga tctgccggag agaatccggc tctagcttgt agatccgctc ccgccccatg
     1921 cgggtttcgg aaaccagccg tgctcgccgc agcacactca aatgcttcga gacagccggg
     1981 cgactgaccc ggaagtcgtc ggccagcgca ttcacgggcg ccggggccgc gcgcaaccga
     2041 tcgagaatcg cccggcgggt ggggtccgcc accgcataga agacgtccgc atgagcgagg
     2101 gcacgtggca ttggcaactc cggtccgttc gtggtcgggc gttgacgcgc tcacgagaaa
     2161 acaagtaacc aattggttac cagtttatgg agtagcctcc cgatcgtcaa ggggaaggaa
     2221 gaatgcgcgg agctcttcag cgagtagctt gggctcttca tgggcgggga agtgcccccc
     2281 acagggcatt tctgtccatc gggtgacgcg gtatgcgcgt tcggcccact cgcgcggcgg
     2341 atgggcaatg tcaccgggga atagcgagac cccgcacggt acttccacgc gctctccggg
     2401 cctcaactgc cgtgggtgca gacgcccttc gtaatacaag cggatcgaag acccgatggt
     2461 ccctgtcacc caatacagtg tgatggtggt gagaagctcg tccaggctga acctgctcgt
     2521 gacgtcgcca tggcagtcag accaggcccg gtacttctcg accagccagg atgcgagccc
     2581 cacaggagag tcatgcaggg cgtacgccag cgtctgcggt ttcgtgcggt ggaggtgggc
     2641 gtacgcgccc tcgacatcgt tccattcccc cgcctcccgc aggaactcac gttcggcggc
     2701 ggtgaggggc ggggcgccgt ggttcagctc tcgttttacc gccgaacctg tcacgtaggg
     2761 gagatgtaca cccaggagat gctccggcgt ttcgagtccg atcagagaga cgacactcgc
     2821 gccgatgtca ctcccatgcg ctccgtatcg ctggtaaccg agcaccttgg tcatgagttc
     2881 gtcatacaac gtcgcaatcc gccccacatg cataccggaa gagctcggaa tatcggagaa
     2941 ggcaaacccg ggcaaggatg ggatgatgag atcgaaaacg tcgtccaaca gggggatcag
     3001 ccgacggtac tcgaggaacg aactgggcca cccatgacag aggaggaggg gaatgcagtt
     3061 ggggtggcgt ccccgcacgt gcacgaagtg aatcccggta ccgcctacca cggcgcgata
     3121 gtgcggccat tcattaatga ggcgctcctg ggtccgccaa ttgaaggagt gaagccagtg
     3181 gtcaaccagg cgttggagaa aatcccgatc ggtcccgtcg caccagggct cggcacggac
     3241 cgggtcgggc cacatagccc gcgctaagcg gctccgaaga tctcggagcg ctgcctccgg
     3301 gacgcggatc gtaaggcggg tgacgccctc ccggtcctcc gcggtctcca ggtggccgcc
     3361 cgcggcaccc gcgagcattt ccgtcaattt ccagcgaact ccgctcactg gacgtccgta
     3421 tgaacgagcg ggtgcttccg ctggacgacg cggcgacggt cgactatccc atacacgacg
     3481 tcagcgctcg attccttcag cgcgagaaac gtcccgttct gcggcctgta gtcgtggacg
     3541 ctccggtggt aggagccatc cgggtcccgt gcctcgccac cgaggatcca gatgtgcgcg
     3601 tgcattgtga caggtgcagg tgacgcagtc cggcccgcgt gaatcgtcga gtcggcgcgt
     3661 ggcgggggcg ctgctgggcg cgctgccctg gccgtcggcg gcagcacgcc gctcaggcgg
     3721 aggtagatcg ccatctggct gtagtgatcg gaccaatgat tgatggtgtt gaagagcgcc
     3781 tgggcccgcg tgccatagtc gggcaacggg tcagccagcc gcgagtcgtt gatgcgggcc
     3841 agcgcccggc gacagaattc gaacgtgtcc ttgaggcggg ccaccagcgc cgccttcccg
     3901 tcattcgcgg tcagcgaggg tcgcgccggc gcggtcgtgt cacccacgac ggccccgatg
     3961 tcgtcattcc cctccaggac gtgcaccacg acttccgcga agctcatttg ggccgtggtg
     4021 gggcggaacg cataccggtt cgccggcatg tcatcggcgg cggcgacgat cctggttgca
     4081 ttgagctcga attccgtacg gtaggaatcc gaaacgaggt gggcgggttg ttgggccgcg
     4141 agcccgatgc taccagccag acccgttgct acggcgagca ccatccgata catgcgactc
     4201 tccagagaat tggggactcg gcaccatcgc cggggcgggg ccaccgacgc ttcgtccgtc
     4261 atcgaagcat tcaggggcac cgtttctatt cttaaccggt tgggtctcta gcgcagggtc
     4321 ttgctctcgc tggtgcttgt gtctagggta accaatcggt tacgagtcat aagcggccgc
     4381 ctgcctggga gtcaaggggc aacgtcgaag acggtacagc accacgtaca cgccgcccgc
     4441 gcaggatttc aacccccgct cgctccgggg caccatgctg ttgcgatggg agtacgttcc
     4501 cggcgccacc ctttctctcg ttttggaccc agcgggggca ggacgtggcg gacaacgggg
     4561 cgctcgacct gggatcgtcg ctcgggcgct cactgccccg caacccgata acgtgctgct
     4621 cgcgaagttc acttactggc tctcctgggg agcccaaagg ccggaggcac cgatgaccac
     4681 ttcgatgacg gacgaaatga tgcgggtgca gatcgcgaac gcccgcgcgt acacgctggt
     4741 gatcctccac ccgacggcga agcggcagga cccgggagcg gaccaggtag tgtgggagca
     4801 tgggcggcgg aatctcgagc tgcggcggga ccggcgcttg tccatcgtgt gcccagtgcg
     4861 ggacgggtcg aacgtttgcg ggatctacgt atttccggga acgcctgagg agacaggcgt
     4921 gatcatggac gatgatcccg gggtgaaggc cggagtcttc acttacgagg ttcatgcgtg
     4981 tcgtggcttc ccgggcgacc acttgccgtg accccgatct ggggcggcac tgtgggtcgg
     5041 tgcctcgacg cacccggccg tccccgccgt gtcccgctcg tcccggattg gttggtgagt
     5101 acaaggcccg agcccgagat tcgttccagc cccaacttgg aacctgccca tgcgtctcgc
     5161 gctcctgctc ctccttgccg tctccactcc ggcggtggcc cagtggccgg tgtacggcgg
     5221 cgatgccggc ggaagccact tttcccagct gcaccaggtc gatcgctcca acgtcccgac
     5281 gttgcggatg gcctggatcg cccgcaccgg tgactacctc accgatcgcg gccgcttcga
     5341 ggcgactccc atcctggtcg acggcactct gtacgtgtcg accccgctgg gcagtgtgct
     5401 cgccttcgac ccggcgacgg gcgccgagtt gtggaagttc gacggaccgg tacggttcgg
     5461 gcacaacgac tacggggact ttgccaaccg cggcgtctcg gcgtgggcgg gtggcagccg
     5521 gggttcgacg tgcgctcatc ggctctacgt cgcgaccgtc aacgcgagac tgctcgcttt
     5581 ggatgcgcgg accggccacc tgtgcgaggg gttcggcgcg cacggcaccg tggacctgac
     5641 ccagggcctg cgacatcgcc cggcctacga ctgggaatac ggggtcacgt caccgccgac
     5701 ggtagtcggt gatctcgtca tcgtgggttc cgcggtggcc gacaaccacc ggaccgatgc
     5761 gcctgagggc gtcgtccggg ccttcgacgt ccggaccggc gcactgcgct ggacgttcaa
     5821 cccgctcccc ggcctgccca acaccggcgc ggcgaacgcc tggagcatct tctccgcgga
     5881 caccgcccgg gatctggtgt tcgtgcccat cggaagcgcg tcgcccgact tctacggcgg
     5941 tgcgcgccca ggggacgacc gttacgcgaa ctcagtggtg gcgctcaggg cgtcgaccgg
     6001 cgcgctggtc tgggccttcc aggtggtgca ccatgacctc tgggatgacg acgtgcccgc
     6061 tcaaccgacg ctcttcacgc tgcatcgtgc gggacggtcc ataccggcgg tggccgtggc
     6121 gacgaagatg gggcatctgt tcattctcga tcgacgcacg ggaacgcccc tcttcccggt
     6181 ggaagaaaag cccgtgccag ccagtgacgt ccccggggag accgcgtcac ccactcaacc
     6241 cttcccgcca ccggcgtttc gactggcccc cgagagcctc tcggccgatc aagcgttcgg
     6301 cgccaacgac tccgtgcggg cggtctgccg tgcccgcatc gcggggctcc gcttccaggg
     6361 ggttttcacg ccgccttcgc tgcagggaac catcatctgg cccggaaaca tcgggggcat
     6421 gaattggtcc ggagtgtcgg tggacgaggc gcggggcatc ctgatcgcac ccaccaatcg
     6481 gctcgcgatg gtggtgcgcc tcattccccg tgacagcctg cacgccgcgt ctatggccca
     6541 gcccgatctc gaatacggga gccagaccgg cacgccgtac gcgtcgagcc ggggcctcct
     6601 gggattctgc acgccgccac cctggggcgc attgacggcc gtcgacctga gcgcgggcgc
     6661 cgtgaagtgg caggtccccc tcggctccat gccgcagatg gcgggcgtcc ccggtgcctc
     6721 cggctccatc cagctgggcg gagcactgat caccgcgggt gggttggtct tcatcgcggg
     6781 aacgttcgac cagcacctca gggctttcga catcgaaacc ggcaaggagc tgtggagcac
     6841 cgggctgccg gccggcgcgc acgcgctccc gatgacctac gaagcggggg gccggcagta
     6901 cgtcgtgatc gccgccggtg gccacgacca cctgcacacc acgatgggcg attacgtcat
     6961 tgcttacgcg ctgccggggc ggggagcccc agtacccgat accagcgccg gcaattggac
     7021 cggggagtgg agcggtgaaa tgcacatcgg ggacgcgaag ttcggcatgc aggtgcggct
     7081 cgcggactcc ggagccacgg cgcagttgga ttcattacag atcacggcgc cggtgcggat
     7141 ggaacggtcg ggccgcggcg tggcgctgtc gtttccgatc tactacgcgg gcaaggcgaa
     7201 gtgcacgggg gacttccgcg tcgagctgga gctctggaat caaggcgcct tgctcgaggg
     7261 gacgggcacc ttcgacggcg cctgcgcttc ccatggacac gagaccgccg cgttcgtgtt
     7321 ccaccggcgc acgcagtagt ttcgcggcat gaccagccgg ctgcgggccg gagccctctg
     7381 ggtgggatcg tttaccgccc tggggctcct catgttcgcg cacttttacc tcgacgtcct
     7441 cgcacgaggg cactcggagc cggtcggcat caagctggtc gaggagctga ccgcgtcgat
     7501 gggcgccggc gtggtcttgc tgcttccgct cgcggtgctc aggcgactcc ggaccgcggg
     7561 ctggtccgga gtccgcctgg tggtggtgca cgcgggactc ctgcccgtct tctccgtgct
     7621 ccacacctcc tggaactggg ctacccgcag cgccctcttt cccctcctgg gcatgggcgc
     7681 gtacgactat ggccggatgc ccgtccggta ccccatggag ttccccaccg acgtcatcgt
     7741 ctatacgttc gtcatcaccc tcatgaccgt gttcatccgg taccgcgaag cccgggaccg
     7801 cgaggtgcgc ctggcgcagg tcgagttcga gctctctcgg gtgcggctcg aagcgctcga
     7861 gggtcagctc cggccgcact tcctgttcaa cgcgctcaac accatttcgt cggtgatgta
     7921 cgaggatgtc gcgctggccg acacgctcct gacccgtctc gccgacctgc tccgtcacac
     7981 cctgcagcat gcggtgggga cggaggtggc gttgcgcgac gagctcgcca ccctcgagct
     8041 ctaccttgca atcatgcgcg cgcgcttcgc cgagcgactg acggtggacg tggacatcga
     8101 tcccctggca ctggacgctc gggtgcctcc cctcgtgttg cagcccctcg tggaaaacgc
     8161 gatcaagcat ggcgacccgg ggccgggagc ccgggcgaac gtgttcatca ccggccgtcg
     8221 agagaacggg cgcctggtcc tgcgggtgga ggacaatggt cccggccttc ccgccgccgt
     8281 cacaccacac gtgggacttg gcgccaccgc ccggaggctc gcggccctgt acggggaggg
     8341 gggaggcgtc gcggtcgaga atcgcgccgg tgggggagcg cgcgcgtctc tgtgggtgcc
     8401 ctaccgggtg gaaacgtgaa cgacggcctg cgggtcctga tcgtggacga cgagcggccc
     8461 gcgcgtcaga agcttcggcg gatcctcggg ggcgagcccg gtgtggaagc cgtgttcgaa
     8521 gcacccgacg gactccgggc ggtcgagctg atcaggagtg aagatccgga cgtggtgttt
     8581 cttgatatcg agatgccggg cgtcgacggc ttccaggtag tcgaagccct gggtgccgag
     8641 agccgtccgc acatcgtatt cgtcacggcg tacgaccggt acgcggtacg cgcgttcgag
     8701 gcgtacgccg tggattacat cctcaagccc tacgatgcgg atcgtctccg caccgcgctc
     8761 acccgggccc gggagcgccg gaacggcccc acgcgcgtgg acgagatggt cgtcgccgtg
     8821 cggagggagc gccccgagcc gctcgagcgt ctcctggtgg aggagggggg ccgggcgatc
     8881 cttctgcccg tcggcagcgt cgaccgcttc gaatcggatc ggaactacgt ggtggtccac
     8941 gccggtaccg gacgataccg gctccgggcc acgctcgatg ggttggagga gcggctggat
     9001 ctgcgcaagt tcgcgcggat ttccagatcg gcgatcgtca atctggaggg catcgtcgag
     9061 ctccggccct gggggcatgg agactacgtg gtggagctca agggcggggt cgagctgagg
     9121 ctgtcgcgac gataccggga ccgtctgaag cacttcgtgc cgtagtggag ccagcagctg
     9181 ggagccgtca gccgacgcgc ccccgtacga gtggaccttc gccgctcaac gtgtgcgatc
     9241 ttagcacggc gtttccgcgt gttgagtgag cggctgctca cgcaagagct ccgctccgat
     9301 cggagcggag ggcttccagt aagtccaatc accactcgct tatcgctgct ctgtccttcc
     9361 tgaaagatag gtaacacggt attccgagca catgggtgac acttttggtc tcctccagag
     9421 gagggggcca tgccttggaa ggagtgtcac gtcatggacg agcggctgcg gtttgtcgcc
     9481 cgcttgctcg agggcgaaaa gatggcgccg ctgtgcgccg cctttgggat ctcccggaaa
     9541 acggggtaca agatctttga gcggtatcag acctgcggac tgggggcctt taccgatcgg
     9601 agtcggcggc cctatcgcca ggcgaatcgc ctgccggccc cgatcgaggc gacgatcgtc
     9661 cggttgaaac gggagtatcc gggctggggc gccccgaaga tccgggagaa gcttcggcag
     9721 caggcgctgg ggattcacct gcccgcgatc agcaccgtcc acgcggtcct ggaccgccac
     9781 ggcttggtgc gccggcgccg gcggcgccgg ggggcggggg ccggcacgcc cctctcgcag
     9841 ccgaccgagc ccaatgccct ctggtgcgcg gattacaagg gggaattcca gctcggcaat
     9901 cgccagtatt gctatccgct cacgatcacg gatttcgcga gtcgctacct gctgacctgc
     9961 gaagcgctct cgactacgca ggagccgttc gcgtttaccg tcttcgagcg gaccttccag
    10021 gaattcgggc tcccccaggc aatccgcacc gacaacgggg tgcctttcgc gtccagtcac
    10081 gccctgtatg ggctgagcaa actggcggtg tggtggctcc ggctcgggat tcggctggag
    10141 cgcatcgccc cggggcagcc ccagcagaat ggccggcatg agcgcatgca cctgacgctg
    10201 aaacaggagg cgaccaagcc cgccgaagcc aatgtgctgc gccagcaggc gcgcttcgac
    10261 gccttcgtca cgcgctacaa cgaggagcgc ccccatcagg cgctgggcat gctcgtcccg
    10321 gcggcggtgt acgtgcgctc cccgcgggtc tatcggggcc tggaggagct cacctacccc
    10381 tatcacgacg ccaccatcac ggtgacccat tgcggccgcc tctgcttcgg cggccgcaag
    10441 atcaacgtca gtcgggtctt ggcgggacaa aatgtcgggg tcctgcaggt gggcgagcac
    10501 gtctggctcg tcacgttcat gcagtatgat ctcggttact tcgacgacga gacctgtcgc
    10561 ctcgagccga tcgcctatcc cttcggctcg aaagtgttac ctatgtgctc ggtataaacc
    10621 gtcacccatg tgaccggaat acacc
//
LOCUS       DASUBP010000149         7284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_862746, whole genome shotgun sequence.
ACCESSION   DASUBP010000149 DASUBP010000000
VERSION     DASUBP010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7284
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862746"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..884)
                     /locus_tag="VFU75_05505"
     CDS             complement(<1..884)
                     /locus_tag="VFU75_05505"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HEU5466325.1"
                     /translation="MSPDRWERDVDDLRRRLVPDHRLGVFDVAVESGALRGATTHPDA
                     LAALRRLAAQAGLSSDLQLLPNVDPAHVAGVVTAAVAPLLAAPDQTAERASELLHGEQ
                     FDVLERRGRWIRVRAPDRYVAWMHDGYCRLGSRDWAEDWVGRATARVIAATILCGGDR
                     RRQLPTGARLAIRSRGGVEMADGEIGDPVGGEVRSDVEFRAQARLIAAPELALRWYGG
                     APYLWGGRSDWGIDCSGLVQTIWAARGASLLRDAAQQIGQGREVTLSDDGSTYQSGDL
                     VFFTVNRRVSHVALWAGAG"
     gene            complement(881..1840)
                     /locus_tag="VFU75_05510"
     CDS             complement(881..1840)
                     /locus_tag="VFU75_05510"
                     /inference="COORDINATES: protein motif:HMM:NF015535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HEU5466326.1"
                     /translation="MTAVSGIAVGHWTHPSGGTGCTVILSPKGGMRAAAAIRGRATGT
                     REFDALAPRHLVPHIDAVLLTGGSAYGLGAADGVMRWLREQGRGLPVSPTQVVPIVPA
                     AVIFDLPADGAARWPRADDAFAACEAAGTTVAEGSVGAGAGATVGKVTGLEHAMKGGL
                     GTWAVQVGDVVVSALVVVNALGDVRGPDGRILAGARGADGKPRDGQAYLAEGGALFGR
                     MAQAVSNTTLAVVATNVEIDRIGLEELGRVGCDALARRITPYGTLFDGDVCFGLSTAE
                     VQARSPLQVQSLVADVMATALERAVRMARGTADVPGLADHAQA"
     gene            1903..2508
                     /locus_tag="VFU75_05515"
     CDS             1903..2508
                     /locus_tag="VFU75_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5466327.1"
                     /translation="MTATATPTSVLVVDDEDSIRQALERFVSRLGYRAVMARDGITAL
                     ERVAQNHFDLMLCDVRMPGVSGVELVPRVLERDPDVAILMLTAVDEPRTAVECLKLGA
                     YDYLIKPVDLDELELAMRTALRRRQLEIERRELERWLASEVQVRTKELEDRTAALEEV
                     SFGALAAARDWPGAAAAIRTLAKELGMTEDEVRKEIRVRGL"
     gene            complement(2505..2687)
                     /locus_tag="VFU75_05520"
     CDS             complement(2505..2687)
                     /locus_tag="VFU75_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466328.1"
                     /translation="MAEKEKLKEHDGMRCRACGNEERASEGYPCQDCGTFICLICTFR
                     GVTRCKACQEKAGAAS"
     gene            complement(2695..4032)
                     /locus_tag="VFU75_05525"
     CDS             complement(2695..4032)
                     /locus_tag="VFU75_05525"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5466329.1"
                     /translation="MADSILLIDDDEGVLRSIGAYFERLGAEVYREGTVKEGLDTFDR
                     MRPDVVVLDLHLPDADGLTALEQLRAKNAAVVLLTGHGDIQTAVQAMQLGAENFLTKP
                     VDMPHLAAAVARISEKVRLRRQNERLAAGVAGDGIDGLGVSSTMQELARQVKLLAASE
                     RTTALLTGESGTGKGWAARLVHRLSPRSAGPFIEVNCAGLSATFLDSELFGHERGAFT
                     DAKEKKQGLFELADGGTLFLDEIGDLAAELQPKLLKVLETKSFRRLGGTRELTVDVRL
                     IAATNRDLVSDVNAGRFREDLYYRLSVMPLRLPAVRDRSREDRIALLTKIMGDLQRQL
                     PGSPPECSTEALDRLLSAPWPGNVREMRNVLERAMILARGQPQIALEHLPAELRRPMA
                     GSRHQPQSLADVEKAQIERALRHHGGNRTRAAKELGISRATLINKIKAYSLDL"
     gene            complement(4022..4978)
                     /locus_tag="VFU75_05530"
     CDS             complement(4022..4978)
                     /locus_tag="VFU75_05530"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEU5466330.1"
                     /translation="MPRVLLITNPAAARTDARAVLSVRDTLRSAGWRVEVLATAAPGD
                     ARRFATEAATERQGGFDALLSVGGDGTAMQVAAGLVGSGIPLGLVAGGTGNILARNLR
                     LPTDPVRAARTILKGAPYTMDLGRVARTDGEHFFAVCAGAGYDAQIMAATDTMFKRRW
                     KIGAYFYKALATLPAVKTVRHRVTVDGQVHIVDAAMVLVANCRELVPRLVKVSPAVWP
                     NDGVFDVIALRADGALDSVVSFLTFLRTRGATDASGRIWSARGRSIKVEVPEGAPRPV
                     QLDGELAGETPFDVEVVPGALTILVDPATVPGGMGKRSSNGR"
     gene            complement(4978..>7284)
                     /locus_tag="VFU75_05535"
     CDS             complement(4978..>7284)
                     /locus_tag="VFU75_05535"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HEU5466331.1"
                     /translation="ASLTLDGALDSLTVTVRGSFDRVRARGLYFSGALTHFQLLPGAV
                     PRFTADVAFDSARGLGERLVGSRAAARGTVDSLTWNLETGLGDLASVAAGGRLRRGQG
                     TTAVGIDSLGVTAPGGAWTLDAPVDVTVNDTAIRVGQISLSQKGSQGKVSGQADLRQN
                     GPGQANLQVVGVPIAGIYALLERDTAGVGGTMTATLGVAGTRSQPTYTGSFAMSGASF
                     GDFSTPYVDGTIGYQDQRLDGTMHVWRSGQRVLNLTAHLPLNLALERVEHRQLADTLA
                     VRAVADSVDLSVLEAVTPTLKRVSGYLSADAGIRGTWDSLRLDGTVAIDSAAATIPSI
                     GARYEQLRGRFRLSGDTIGVESLTARSDRGTATVTGVVRLDQLSKPVLALHISADRFK
                     ALDIRNSLTVTATGELDLTGPVFGATVRGRGTVTSGVLYFADLVNKRVINLDSPDPWI
                     ASLIDTSLAGLIKRERLGPAFQSVFLDSLRVDGLHLTMGNDVWLRSNEANIQLAGAVD
                     MNKRLKNYLISGTLQTPRGTYRLTLKPVTKDFTVTQGTVTFFGTPDLDAGLNIRATHV
                     VHTNTAAGLSAGPENITVIANIGGSLLVPKLTLSADQPNMSQTEIISYLLFGQPSAPI
                     GGDQGAGPSRSAMVTSAVASLVSGQLEQSVVSDLGIPIDYFELHPESGTGAGLSGAQL
                     AAGWQIGRKTFLVLNAGYCGSTNGNSSSFDLSSTLGASLQFRISPEWRTEASFEPVTN
                     CNQTFLNGLAGLGATRQIGLDLFWERRY"
BASE COUNT         1182 a         2635 c         2366 g         1101 t
ORIGIN      
        1 cccgcgccgg cccagagtgc gacgtgcgag actctccgat tgaccgtgaa gaacaccaga
       61 tctccggact ggtaggtcga gccgtcgtcg gagagcgtga cctcccgccc ctgcccgatc
      121 tgttgcgcgg cgtcccgcag caacgatgcg ccccgcgcgg cccagatggt ctgcacgagt
      181 ccggagcaat cgatgcccca gtcactccgg ccgccccaca ggtacggcgc accaccgtac
      241 cagcgcaacg caagctccgg cgcggcgatg agcctcgctt gcgcgcggaa ctcgacgtcg
      301 ctgcgcactt cgccgcccac cggatcaccg atttctccgt cggccatctc cacgccgccg
      361 cgcgagcgga tcgccagacg cgcgccggtc ggcaactgcc ggcggcggtc gcctccgcac
      421 aggatcgtcg ccgcaatcac ccgcgcggtc gcccgcccca cccagtcctc ggcccaatcc
      481 cgcgaaccca gccggcagta cccatcgtgc atccacgcga cataccggtc tggagcgcgg
      541 acgcggatcc acctcccacg gcgctccagc acgtcgaact gctcaccgtg caggagctcg
      601 ctcgcgcgct ccgccgtctg gtcgggagcg gccaggagcg gagccaccgc cgcggtcacg
      661 acacccgcca cgtgcgcggg atcgacgttg ggaagcaact gaaggtccga cgagaggcct
      721 gcctgggcgg cgaggcgccg cagggccgcg agcgcgtcgg gatgcgtggt cgcaccacgg
      781 agtgcccccg actccaccgc gacgtcgaac acgcccaagc gatggtccgg caccaggcgg
      841 cgccggagat catcgacgtc acgctcccag cggtcgggac tcatgcctgc gcgtgatccg
      901 cgagccccgg aacgtccgcg gtaccgcgcg ccattcggac cgcacgttcg agtgccgtcg
      961 ccatcacgtc cgccaccagg gattgcacct gaaggggaga tctggcctgg acttccgcgg
     1021 tactcagccc gaagcagacg tctccgtcga acagggtgcc atagggcgta atccggcgag
     1081 ccagggcgtc acaccccaca cgtcccagtt cctccagccc tatgcgatcg atctccacat
     1141 tggtcgcgac gaccgccagg gtcgtgttgg acaccgcctg cgccatccgt ccgaagaggg
     1201 cgccaccctc ggccaggtac gcctgaccgt cccgcggttt gccgtcggcc ccgcgcgcgc
     1261 cggcgaggat gcgcccgtca ggcccacgca cgtcgccgag ggcgttcacg acgaccaacg
     1321 cggacacgac cacgtccccc acctgcaccg cccaggtacc cagaccgccc ttcatcgcgt
     1381 gctcgaggcc tgtgaccttc ccgacggtgg ctcccgcgcc tgctccaacg gatccctcgg
     1441 cgaccgtcgt tcccgcggcc tcgcacgccg cgaaggcatc atcggccctg ggccaccggg
     1501 ccgcaccgtc ggccgggaga tcgaagatca cggccgccgg aacgatcggg accacctgcg
     1561 tcggggacac cggaagccct cgcccctgct cccgcaacca ccgcatcacg ccgtcggctg
     1621 cccccagacc gtaggccgag ccaccggtca ggagtacggc gtcgatgtgc gggaccaggt
     1681 gccggggcgc cagggcgtcg aactcgcggg tgcccgtcgc gcgtccccgg atggcggccg
     1741 cggcccgcat cccccccttg ggggagagga tgaccgtgca cccggttccc ccggaaggat
     1801 gcgtccaatg gcccaccgcg atcccggaaa ccgccgtcaa ggctggaaca aatggggatt
     1861 tcatttgcgt ccgaagatag cgctgaatac cttcgtggcc acatgaccgc caccgcgact
     1921 ccaaccagcg tcctcgtcgt cgacgacgag gactccatcc ggcaggccct ggagcgcttc
     1981 gtgagccggc tcgggtaccg cgccgtgatg gcccgggacg gcatcaccgc tctcgaacgg
     2041 gtggcgcaga accatttcga cctcatgctc tgcgacgtcc gcatgccggg cgtgagcggc
     2101 gtggagctcg tgccccgcgt gctcgagcgt gatcccgacg tcgcgatcct gatgctcacc
     2161 gcggtcgacg agccccgcac cgccgtcgaa tgcctgaaac tcggcgccta cgactacctt
     2221 atcaaaccgg tggatctgga cgagctggag ctggcgatgc ggacggccct tcgccggcgg
     2281 cagctggaga tagagcggcg ggagctggag cggtggctgg ccagcgaagt gcaagtccgt
     2341 accaaggagc tcgaagaccg gacggcggcg ctggaagagg tgtcctttgg cgctctggcc
     2401 gcggcccgcg actggccggg ggccgcggcc gcgatcagga ccctcgcgaa agagctgggg
     2461 atgacggaag acgaggtgcg gaaggaaatc cgggtgcgcg gactctagct cgccgcgccg
     2521 gccttttcct ggcaggcctt gcaccgcgtc acgccccgga aggtgcagat caggcagatg
     2581 aaggtcccgc agtcctgaca cggatacccc tcggacgccc gctcttcgtt cccgcacgcg
     2641 cggcagcgca tcccgtcgtg ctctttgagt ttctcttttt ccgccatcgg aacctcagag
     2701 atccagtgag tacgccttaa tcttgttgat caacgtcgcg cgcgaaatgc cgagctcttt
     2761 ggcggcccgc gtgcggttgc cgccgtgatg ccgcaacgcc cgttcgatct gcgctttttc
     2821 gacgtccgcc agactctgcg gctgatgccg cgatcccgcc atgggccgcc ggagctccgc
     2881 aggcagatgc tcgagcgcga tctgcggctg cccccgcgcc agaatcatgg cccgctccag
     2941 aacgttgcgc atctcccgga cgttcccggg ccagggcgcc gagaggagcc ggtcgagcgc
     3001 ttcggtcgag cattccggcg gagagccggg aagttgccgt tgcaggtccc ccatgatctt
     3061 ggtcaacagg gcaatccggt cttccctcga gcggtcccgg acggcgggca gccggagcgg
     3121 catcacggag agccggtagt agagatcctc acggaaacgc ccggcgttca catcgctgac
     3181 cagatcccgg ttggtcgccg cgatcagccg gacatccacc gtcagctccc gcgtgcctcc
     3241 gagccgccgg aacgacttgg tctcgagcac cttcaatagc ttcggctgca gctcggccgc
     3301 gagatcaccg atctcgtcca gaaacaacgt cccgccatcc gccagctcga agagtccctg
     3361 cttcttctcc ttcgcgtccg tgaacgcacc gcgctcatga ccgaagagct ccgagtccag
     3421 gaacgtggcc gacagcccgg cgcagttcac ctcgatgaac ggaccggccg atcgcgggct
     3481 caggcgatgt accaggcgcg cggcccagcc cttccccgtc ccgctctctc ccgtgaggag
     3541 cgccgtcgtg cgctcgctgg cggccaggag tttcacctgc cgggccagct cctgcatggt
     3601 cgacgaaacg ccgagcccgt cgatgccgtc cccggcgacg ccggcggcga gccgttcgtt
     3661 ctggcggcgc aggcggacct tctccgaaat tcgcgcaacc gcggcggcca ggtgcggcat
     3721 gtccaccggc ttggtcagga agttctccgc acccagctgc atcgcctgca cggccgtctg
     3781 gatatcgcca tgccccgtca ggagcacgac ggcggcgttc ttggcccgca gctgctcgag
     3841 cgccgtcagg ccgtcggcgt cgggcagatg caggtcgagt acgacgacat cgggccgcat
     3901 gcgatcgaag gtgtccagcc cctccttcac ggtcccctcg cgatagacct cggcgcccag
     3961 ccgctcgaag tacgcgccga tggagcgaag gaccccttcg tcatcgtcga tgagcagaat
     4021 gctatcggcc attcgagctc cttttcccca tgcctccggg gacggtggct ggatccacga
     4081 gaatggtcag cgcacccggc acgacttcaa cgtcgaaggg cgtctccccc gccagctcgc
     4141 cgtcgagctg aacaggacgc ggcgcaccct ccggaacctc gaccttgatg gagcggcccc
     4201 gggcactcca gatccgtccc gaggcgtcgg tcgcgccgcg ggtgcggagg aacgtcagga
     4261 aggacacgac gctgtcgagc gccccgtcag cgcggagggc gatcacgtcg aatacaccgt
     4321 cattgggcca gaccgcgggc gacaccttca cgagccgtgg gaccagctcc cggcagttcg
     4381 cgaccagcac catcgccgca tcgacaatgt gcacctgccc gtccaccgtc acccggtgcc
     4441 gcaccgtctt gaccgccggg agcgtggcga gggccttgta gaaataggct ccgattttcc
     4501 agcgccgttt gaacatggtg tccgtcgcgg ccatgatctg cgcgtcatag ccggcgccgg
     4561 cgcagaccgc gaagaaatgc tccccgtcgg tgcgagccac ccgccccaga tccatggtgt
     4621 acggagcgcc cttcagaatc gtccgggcgg cccgtaccgg atcggtcggc agccggaggt
     4681 tccgcgcgag gatgttgccg gtgccgccgg cgacgagccc cagcgggatt ccgctcccca
     4741 ccaggccggc ggcgacctgc atggccgtcc cgtcaccgcc caccgacagc aaggcgtcga
     4801 acccaccctg tctctcggtg gccgcttcgg tggcaaaccg ccgcgcatcg ccgggcgccg
     4861 ccgtcgccag tacttcgacg cgccaccccg cgctccggag tgtgtctctc accgagagca
     4921 cggcccgggc gtcggtccgc gccgcggccg gattggtgat gagcaggacg cggggcacta
     4981 gtagcgccgc tcccagaaca gatcgaggcc gatctgacgc gtggccccga ggccggccaa
     5041 cccgttcagg aacgtctgat tgcagttggt gacgggctcg aaactcgctt cggtccgcca
     5101 ctccggactg atccggaatt ggagactcgc gccgagcgtg ctgctcaagt cgaacgaact
     5161 gctgttgccg ttggtgctcc cgcagtaacc cgcgttcagc accaggaagg tcttccgccc
     5221 gatctgccat cccgccgcca actgcgcacc ggagagtccg gctccagtcc cactctcagg
     5281 atgaagctcg aagtagtcga tgggaatgcc gagatcggaa acgacgctct gctccagctg
     5341 acccgacacc aggctggcga ccgcggatgt caccatcgcg ctgcggctgg ggcccgcccc
     5401 ttgatcgcct ccgatcgggg ccgacggctg gccgaacagc agatacgaga tgatctcggt
     5461 ttgcgacatg ttgggctggt cggccgacag tgtcagcttc ggcacgagca acgaaccccc
     5521 gatgttggcg atcaccgtga tgttctcggg tcccgcgctc agtcccgccg cggtgttggt
     5581 atgcaccacg tgcgtcgcgc ggatgttcag tccggcgtcg agatcggggg tcccgaaaaa
     5641 cgtcaccgtc ccttgcgtca ccgtgaagtc cttggtgacg ggcttcaggg tcagccggta
     5701 ggtgccccga ggcgtctgca gggtgcctga gatcaggtag ttcttcagcc gtttgttcat
     5761 gtccacggcg ccggcgagct ggatgttcgc ctcgttcgac cggagccaca catcgttgcc
     5821 catcgtcagg tgcagtccat cgacccgcag gctgtccagg aatacgctct ggaaagcggg
     5881 acccagccgc tcccgcttga tcaggccggc cagggacgtg tcgatcaggg acgcgatcca
     5941 tggatcgggg gaatccaggt tgatcacgcg cttgttcacg agatcggcga agtagagcac
     6001 gccgctcgtc accgtgcccc gcccccgcac cgtcgcgccg aagacggggc ccgtcaggtc
     6061 cagctctccg gtcgccgtga ccgtcagaga gtttctgatg tccaacgcct tgaaccggtc
     6121 cgccgagatg tgcagggcga gcaccggctt cgacaactga tcgaggcgga ccacgccggt
     6181 cacggtggcg gtgccccggt cactgcgagc cgtgagggat tccaccccga tcgtgtcacc
     6241 cgaaagacgg aaccggcccc gcagctgctc gtagcgggcc ccgatggacg ggatggtcgc
     6301 cgccgccgag tcgatcgcaa cggtgccgtc gaggcggagc gagtcccacg tgcccctgat
     6361 gccggcgtcc gccgagaggt aaccggagac tcgcttcagg gtcggggtaa cggcttccag
     6421 gacggagagg tcgacagaat cggccaccgc ccgaacggcg agggtgtcgg caagctgacg
     6481 gtgctccacc cgctcgagtg ccaggttgag cgggaggtgg gccgtcaggt tgagcacccg
     6541 ctggcccgac cgccagacgt gcatggtgcc gtcgagtcgc tggtcctggt acccgatggt
     6601 cccatcgacg tagggcgtgc tgaagtcccc gaacgacgcg ccggacatgg cgaatgatcc
     6661 cgtatacgtc ggctgcgacc gggtgccggc gacgccgagc gtcgcggtca tggtcccacc
     6721 gacaccggcc gtgtcacgct ccagcaaggc gtagatcccg gcgatgggaa ccccgaccac
     6781 ctgcaggttg gcctggcccg gaccgttctg acgcaggtcg gcctgaccgg acaccttgcc
     6841 ctgactgccc ttctgtgaga gcgaaatctg accgacccga atcgccgtgt cgttgaccgt
     6901 cacgtccacc ggcgcgtcca gcgtccaggc acctccagga gcggtcaccc ccagggagtc
     6961 gatgcccacc gcggtcgtac cctggccccg gcggagccgt ccgcccgccg ccaccgaagc
     7021 gagatctcca agtccggtct cgaggttcca ggtcagcgag tcgacagttc cccgggcagc
     7081 ggcgcggctc cccacgagcc gttcgcccag tccgcgcgcc gaatcgaacg ccacgtcggc
     7141 cgtgaagcgc ggcacggcgc ccggcaggag ctggaagtgg gtcagcgctc cggagaagta
     7201 cagcccgcgg gcgcgcaccc ggtcgaatga gccccgcacc gtcacggtca gcgaatcgag
     7261 ggccccgtcc agcgtcaggc tcgc
//
LOCUS       DASUBP010000150         9554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_903608, whole genome shotgun sequence.
ACCESSION   DASUBP010000150 DASUBP010000000
VERSION     DASUBP010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9554
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903608"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..624)
                     /locus_tag="VFU75_05540"
     CDS             complement(52..624)
                     /locus_tag="VFU75_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466332.1"
                     /translation="MANTASRAASGALSRSLRRPVSVTVPEFAVSLLEQLPAVLGHGA
                     RPLVAVRAELLGDISGPLAWVMTRTEAARMAGILLARESGSVPLNDMATQAVLTRSAH
                     TLAGGYADVLAALTHGVVFLSVPEMAEGTIDRLLVRRSVGPEARQSDQLTVCVGSRIG
                     FDDQNLAVMGHLVFLPHHPTFGRILRALAD"
     gene            814..2787
                     /locus_tag="VFU75_05545"
     CDS             814..2787
                     /locus_tag="VFU75_05545"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HEU5466333.1"
                     /translation="MLLRDFRNFIAVAETRSLSRAAARAHLAQPALSRQIHELERELG
                     VPLLERHSKGVTPTAAGQAFARGAANLLVTLGFAIDRAEATSEGRRGRVSIGAMLTII
                     AAGIPAAVEERVHQEDPDITLAVEDLDPPDTVEQVQAGAIDIALAMAVGDDPELVVEP
                     LWDEYLDHIILHHGHPLAGRRRLKLSDLADYPLIIPQRALAPGILDEIMAVLREGGLR
                     SPLLVVDVGLRDGHLAVSAGRGWSLIGRARSVSPPQGTTVVALADAAPKVRAAAIWRR
                     DDKRPVLRRVLEHILDAVRDHPHACLRREPHLPPSAPARKARRAPLGSVPTAMEIRHL
                     RALLEVAAAQTIGRAAERLGVTQPALSRQLKELEHATGLALLERTARGVLLTPAGNSL
                     AGECPGILSGLEQIVLDTTRARRGMEGRCVIGAVATVTTGEMLTRVLTEVAQRHPHVH
                     VVIEEISTPLQAPAIVRGDIDLGLAHAHPGVVHPGLIVEHVFDDRLQMALLPAGHPLA
                     RRARVKPAELADMPFLFPDRDFQPAFYDRVFDTLHDAGLIPRVDGTHAGLQAIWALVA
                     RGKGWGLGFKSHRRRPPGGTVAVPLVGFDLPWGLDLLRRRGEPSIALRCALEAFRRAA
                     RSGGDPTTPASRPTATRRGDRGHRGRSSRVTER"
     gene            complement(2811..3824)
                     /locus_tag="VFU75_05550"
     CDS             complement(2811..3824)
                     /locus_tag="VFU75_05550"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5466334.1"
                     /translation="MTTGAEAVDLRELRTLAESGRYREVLARLERLPLPDVEKRTPLS
                     LLAAEAHGRMGELSAAARWAALALETSRQRGEPQAELRARNYQGLIALRQGDIDAAER
                     HLTAALEMARAQSDALFEARCLNNLGVIANLRGDRTQALADYQLALAAYQQAGLVRGM
                     AETHHNIGITLRDMGDHQRALAAAEHAVRLARMVKDDTLIGLTLFGRAAIHLQQTDSS
                     LAGVELERAAEAYRRVEFESGLAEVWRLQASVLRLKGNLAGALDLLRRAADLARERGS
                     ADTVADIDRDLGSALAVTGDTDGAREAWGRAAAGYRRLGAVKAAEDVDALVTKPSPPG
                     SPR"
     gene            complement(3852..4466)
                     /locus_tag="VFU75_05555"
     CDS             complement(3852..4466)
                     /locus_tag="VFU75_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466335.1"
                     /translation="MRRLQWLMLAAVVGCTDATTSPSAPPVTTTPNSQLHIVAQDTTA
                     PALLSDSVSFYAVAGQGRQVKMYYQGASPGDTGETLLEFEVPGDGLLKKPDGTSFQPG
                     DSILITISVATPGRFEFTFGPTGLLFNPASPARLKIEYGHCDHDFNDDGHLDPADSTT
                     QTLLDVWRRQTGDTLWTKLGGVNLDEDNEVSVNVLHFTQYALAW"
     gene            complement(4585..5997)
                     /locus_tag="VFU75_05560"
     CDS             complement(4585..5997)
                     /locus_tag="VFU75_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5466336.1"
                     /translation="MGTGVSLTTGSTPRAGSSAEPRPPTWFLVVDLSDSFSDHWRALA
                     AALGAELRRATPPESPSASVGAVAVIVAAGGVEPDIPQAIPALAVPPGTPVFAVGAAV
                     SHRVAARAVAAGAADYFALPGDRDALQSALEAAIDRRRSALDRAALAHLEAQSHAFKE
                     IVGESPALHKALDRAARVLPHADATVLITGETGTGKELLARAVHYGGPRATAPFVELN
                     CAALPPQLLESEIFGHERGAFTDAKGSKPGLLEVAHGGTLFLDEVDRLTPELQGKLLR
                     AIELKTARRLGATTSRTFDVRIVAATNADLSTAVHEGRFREDLYYRLKVVTLSLPPLR
                     ERGDDVIRLAEFFIEKLSGQYGLPRPAITKEVRAVILAQPWPGNVRELRNTIERVILL
                     SPPGTLDITELNAPGSAAPTRSGTLPFPARLAEIQRAAAHAMLKETDGNHSEAARRLG
                     ISRSRLARLLKGVDDTPDSD"
     gene            complement(6113..6532)
                     /locus_tag="VFU75_05565"
     CDS             complement(6113..6532)
                     /locus_tag="VFU75_05565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466337.1"
                     /translation="MYSALVAILMMFQPAAKTAGCVIVVNDNNQFYTASTGQVADLFL
                     AKRTTWQNGSAVTPVDQMPEAQVREEFSVAVLGRPLQAVQAYWRAKTYREDVTPPPEL
                     LNDQAVLDYVKTHPNAIGYVTPDADLPAGVHAVVVTR"
     gene            6689..6761
                     /locus_tag="VFU75_05570"
     tRNA            6689..6761
                     /locus_tag="VFU75_05570"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6722..6724,aa:Lys,seq:ttt)
     gene            complement(6775..7851)
                     /locus_tag="VFU75_05575"
     CDS             complement(6775..7851)
                     /locus_tag="VFU75_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466338.1"
                     /translation="MNRTLAVGLLFIVLGACRETAAPVPAPTTPPPDTSVRGTLRVLT
                     IPTADGTGRVVHPDYVRMPASWPGSPRYLAITTYPAADPTREWPSLFSQDSACTHWHL
                     PAGGPNPVLQPPTAMTYTDPDVVYVPDSNQIWMYVRLVTPMWNVLQLIRSGDGVNWTS
                     PVDIDSAVNHGMISPAVVRRGPGDWYLWSVHAGAGGCAVAGSAFLQRRTSTDGVHWSV
                     PDTARIAGHPWHVDVQWIPDRKEWWALYVESCLPTAVRLATSPDGLAWSVVPSPVITA
                     GVIPQFGDVVYRSTFAVEGDSVRFWFSGARWDSVSDSGWVWRAAASIEGTSALLERLS
                     QPAPVAPRRFVAPRGQLVPYPEAP"
     gene            7951..8814
                     /gene="ilvE"
                     /locus_tag="VFU75_05580"
     CDS             7951..8814
                     /gene="ilvE"
                     /locus_tag="VFU75_05580"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF006185.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HEU5466339.1"
                     /translation="MPTTIWINGEYKTRDEASVSVFDHGLLYGDGVFEGIRAYGGRIF
                     RLSEHIDRLYSSAKSIWLDPGMPQADMRKMVEDAQAKSGIKDSYIRLVITRGTGDLGM
                     DPRKCDKPTVFCIVDTIALWSKERYERGLTCITAATPISHKENLSPRVKSLNYLSHIL
                     AKIEGIAANVDEVIMLDSSGYVAEASGMNLFCARRGVVKTPPPYSGILRGVTRDAMIE
                     MARDANYEVVEEPLNRYDLYSADEVFLTGTAAEIVAVTKLDGRVIGNGQSGPVVADLG
                     RRFRALVTRGD"
     gene            9218..9466
                     /locus_tag="VFU75_05585"
     CDS             9218..9466
                     /locus_tag="VFU75_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466340.1"
                     /translation="MNAVDLHDLRRAAAAVARLRGETVKDVTARSDLRQLKVELESGL
                     ILVVSAERDEQNRPHLEVDVVEAPQAASSRHQIEVRFD"
BASE COUNT         1537 a         3112 c         3329 g         1576 t
ORIGIN      
        1 cgcatgcggg aatgcccgcc ggcgaggggg gcaggggaaa ccgtcgggtc atcagtcagc
       61 cagcgcgcga agaatgcggc cgaaggtcgg atggtgcggc aggaatacga ggtgccccat
      121 caccgcgaga ttctggtcgt cgaaaccgat gcgagacccg acgcacacgg tcagctgatc
      181 ggactggcgc gcttccggcc cgaccgaccg ccggaccagc aaacggtcga tggtgccttc
      241 ggccatctcg gggacggaga gaaagaccac gccgtgcgtc aacgctgcca acacgtccgc
      301 gtagccgccg gcgagtgtgt gcgcgctccg cgtcagcacc gcctgcgtcg ccatgtcgtt
      361 gagcgggacg gatcccgact cgcgggccag gagaatgccg gccattcggg ccgcctcggt
      421 acgagtcatg acccacgcga ggggaccgga aatgtctccc aggagttctg ccctcacggc
      481 gacgagcggg cgggcaccat gccccaggac cgcgggaagc tgttcgagca gggaaaccgc
      541 gaactccgga acggtgaccg acaccgggcg gcgcagagac cgggacagcg ccccggacgc
      601 cgcgcggctg gcggtattcg ccacttcgcg cagcgcaccc agatggcgcc ccggctcgca
      661 ggcctctagc gacagtgttt cagcgaacgt atggtcactc ctgcgcctgg tggcgcgaaa
      721 tcgtcgacgg tcatcctagc ggggtagaca ggccggcagg aaataccgat ccgctagggc
      781 ttcgatgccg tcggcgcatg gccaaggggt ccgatgctcc tgcgggattt ccggaatttc
      841 atcgccgtcg ccgagacacg gagtctctcc cgtgccgccg cacgcgctca cctcgcccag
      901 ccggcccttt cccgacagat ccatgagctc gagcgcgagc tcggcgtccc gctgctggaa
      961 cgtcattcca agggcgtcac gccgaccgcc gcggggcagg cttttgcgcg aggagccgcc
     1021 aacctcctcg tcacgctggg tttcgccatc gaccgggcgg aggcgaccag cgaaggccgg
     1081 cggggacgcg tttccatcgg ggcgatgctc acgatcatcg cggccgggat tccagcggcg
     1141 gtagaggagc gggtccatca ggaggatccg gacatcaccc tcgccgtcga ggacctcgac
     1201 ccgccggaca ccgtggaaca ggtccaggcc ggcgcgatcg acatcgcgtt ggccatggcg
     1261 gttggggatg atccggaact ggtcgttgag ccgctctggg acgagtatct cgaccacatc
     1321 attcttcatc acggacaccc gctcgcgggc cgccgtcggc tgaagctgtc ggatctcgcc
     1381 gactacccgc tcatcattcc ccagcgcgcg ctggcccccg ggattctcga cgagatcatg
     1441 gcggtgctgc gggagggcgg gctgcgctca cccctgctcg tggtcgacgt cggattgcga
     1501 gatggccacc tggcggtgtc ggccgggcgc gggtggtctc tcatcggcag ggcgcggtcg
     1561 gtatcgccgc cgcaggggac gaccgtggtc gccctggcgg acgcggcgcc caaggtccgg
     1621 gcggcggcca tctggcgtcg tgacgacaag cgccccgtcc tgcggcgggt gctcgagcat
     1681 atcctcgacg ccgtccgcga ccacccccac gcgtgcctcc gccgtgaacc ccatctcccg
     1741 ccatcggctc cggcgcggaa agccagacgg gcgcccttgg gctcggtccc gacggcgatg
     1801 gaaatccgcc acctccgggc attgctcgag gttgcggccg cacagacgat cggccgtgcc
     1861 gccgaacgcc tcggcgtcac ccagccggcg ctctcccgcc agctcaagga gctcgagcac
     1921 gcgaccggtc ttgcgcttct ggagcggacg gcgcggggcg tgttgctgac cccggcgggg
     1981 aattcgctgg cgggggaatg tcccggcatt ctgtcggggc tcgagcagat cgtcctcgac
     2041 acgacgcgcg cccggagggg catggaaggg cggtgcgtca tcggcgccgt ggccaccgtc
     2101 acgacgggcg agatgctcac gcgcgtgctg acggaggtcg cgcaacgaca tccccatgtg
     2161 cacgtggtca tcgaggagat ttcgaccccc ttgcaggctc ccgccatcgt tcgcggggac
     2221 atcgatctcg gcctggccca cgcgcaccct ggagtcgtgc atcccggact gatcgtggag
     2281 cacgtgttcg atgatcggtt gcagatggca ctgctgccgg ccggtcatcc gctggctcgt
     2341 cgggctcgtg tgaagccggc agaactggcc gacatgccgt tcctgtttcc cgaccgcgac
     2401 ttccagccgg cgttctatga ccgggtcttc gacacgctgc acgatgcggg gttgatcccc
     2461 cgggtggatg gaacccacgc tggactccaa gccatctggg cgctggtggc acgggggaaa
     2521 gggtggggac tgggcttcaa gtctcatcgc cggcgaccgc caggcggcac ggtcgcggta
     2581 ccgctggtgg ggttcgatct gccgtggggg ctcgacctgc ttcggcgccg cggggagccg
     2641 agtatcgcgt tgcgatgtgc tttggaagcg ttccggcggg cggcccggag tgggggggac
     2701 ccaaccacgc ccgcgtcccg accgacggcc acgcgacggg gggatcgagg ccatcgtgga
     2761 cggtcatcgc gagtgactga gcggtagggg gcggcccggc cgccggattc ctaccgcgga
     2821 gacccggggg gcgatggctt cgtgacgagc gcgtccacgt cttcggcggc cttcacggca
     2881 cccagccggc ggtagcccgc ggcggcccgc ccccatgctt ctcgggcgcc gtcggtatcc
     2941 ccggtgaccg ccagggcgct gcccaagtcg cggtcgatgt cggcgaccgt gtccgccgag
     3001 ccgcgctcgc gcgcgagatc agccgcccgc cgaagcagat cgagtgcccc ggcgaggttg
     3061 cccttcagcc gcaacactga ggcctgcaac cgccacacct ccgccaaccc cgattcgaat
     3121 tccacccggc ggtacgcctc ggcggcccgc tcgagctcca caccggccaa ggagctgtcg
     3181 gtctgctgaa ggtggatcgc cgcccgtccg aaaagcgtca acccgatgag cgtgtcgtcc
     3241 ttgaccatgc gcgcgagccg caccgcgtgc tctgcggccg cgagcgcccg ctgatgatcc
     3301 cccatgtcac ggagtgtgat gccgatgttg tgatgcgtct ccgccatccc gcgcaccagg
     3361 ccggcctgct gatacgcggc gagggccaac tgataatcgg cgagcgcctg cgtccggtct
     3421 ccccggaggt tcgcgatcac gccgaggttg ttgagacacc gcgcttcgaa cagcgcgtcc
     3481 gactgcgccc gggccatctc gagcgcggcg gtcagatgcc gttcggcggc gtcgatgtcc
     3541 ccttgccgga gggcgatcag tccctggtag ttccgggccc gcagctcggc ctggggttcc
     3601 ccgcgttggc gcgacgtttc caatgccagt gccgcccacc gggccgcggc ggaaagctcg
     3661 cccatgcggc cgtgggcttc ggcagccagg agcgaaagcg gggtccgctt ctcgacgtcc
     3721 gggaggggca gacgctcgag tcgggccagt acctcgcggt accgcccgga ctcggcaagc
     3781 gtcctgagtt cccggaggtc cacggcctcc gcgccggtcg tcatcccgga ctcccgatca
     3841 tcctgccgcg ctcaccaggc gagggcgtac tgggtgaaat ggaggacgtt gacgctgact
     3901 tcattgtcct cgtcgaggtt cacgccaccc agtttggtcc acagggtgtc cccggtctgc
     3961 cggcgccaga cgtccagcaa ggtctgcgtc gtggaatcgg ccggatcgag gtggccatca
     4021 tcattgaagt cgtgatcgca atgaccgtac tcgatcttga gacgggccgg tgatgcggga
     4081 ttgaacagca gaccggtggg cccgaacgtg aactcgaacc gccccggggt ggccaccgag
     4141 atcgtgatca ggatggagtc gcccggctgg aacgacgtgc catcaggctt cttcagcagg
     4201 ccgtctcccg ggacttcgaa ctcgagcagg gtttctccgg tgtccccggg tgaggcgccc
     4261 tggtaataca tcttcacctg gcgcccctgg ccggcgacgg cgtaaaagga cacgctgtcg
     4321 ctcagaagag cgggggcagt ggtatcctgc gcgacaatgt gcagctggga attcggcgtg
     4381 gtggtgaccg gtggcgctga cggggacgtc gtcgcatcgg tacaaccaac cacagcggcc
     4441 agcatgagcc actgtagcct tcgcatcatg ggtctccggg tgtcagggtg ttgcgctctt
     4501 gatttgcaag gcttacgcca agcgtaagcg tttgcgctgt cgtgggttgg gtctggagaa
     4561 atgactcggc gcgagccaac tggctcaatc cgagtcaggc gtgtcgtcca cacccttcag
     4621 caggcgggcc agccgcgatc gtgagatgcc aagacggcgc gccgcttcgg agtgattgcc
     4681 gtccgtctcc ttgagcatgg cgtgcgccgc ggcccgctgg atttcggcta gtcgggcagg
     4741 gaacggcagc gtccccgagc gcgtcggcgc ggcgctcccg ggggcattga gctcggtgat
     4801 gtccagcgtg ccgggcggcg agagcaggat cacccgctcg atggtgttgc ggagctcgcg
     4861 cacgttgccg ggccagggtt gggccaggat cacggcgcgg acctccttcg tgattgccgg
     4921 tcgggggagg ccgtactgcc cggagagctt ttcgatgaag aactcggcga gccggatcac
     4981 gtcgtccccc cgttcgcgaa gcgggggcag cgacagcgtg acgaccttca gccggtaata
     5041 caggtcttct ctaaaccgac cctcgtgcac cgcggtgctc aggtcggcat tcgtggccgc
     5101 gacgatccgg acgtcgaatg tgcgactggt cgtggcgccg agtcgccggg cggtcttgag
     5161 ctcgatggcg cgcaggagct tgccctgcag ctcgggggtc aggcggtcga cctcgtcgag
     5221 gaagagcgtc ccgccatgcg cgacttccag cagtccgggt ttggagccct tggcgtccgt
     5281 gaatgcgccg cgctcgtgcc cgaagatttc gctctccagg agctggggag ggagcgctgc
     5341 gcagttgagt tcgacgaagg gtgccgtggc tcgcggtcca ccgtaatgca cggcgcgggc
     5401 gagcagctct ttgccggtcc ccgtttctcc ggtaatgagg accgttgcat ctgcgtgggg
     5461 caggacgcgc gccgcgcgat cgagcgcctt gtgcagggcc gggctctcgc cgacgatttc
     5521 cttgaaggcg tgcgactggg cttcgaggtg ggccagagcg gcacggtcga gggcactccg
     5581 gcgccgatcg atggcggcct cgagtgcgga ctgcagggcg tcccggtcgc cggggagcgc
     5641 gaagtagtcg gccgctcccg cggccacggc ccgcgctgcc acccgatgcg agacggccgc
     5701 gccaaccgcg aacacgggcg tgccgggtgg aaccgcgaga gccgggatgg cctggggaat
     5761 gtcgggctcg accccacccg ccgcgacgat tacagcgacg gctccgaccg acgcggatgg
     5821 agattccggc ggcgtggccc tccggagctc ggcacccaaa gcggcagcga gcgctctcca
     5881 atggtccgag aaggagtcgc tgaggtcgac gacgaggaac caggtgggcg ggcgcggctc
     5941 ggcggacgag ccggcccggg gggtcgatcc ggtagtcaga gatactcctg ttcccatggg
     6001 gcgccgatgc tacgaaggaa agttctaccc cagggttaca gagaggtaca gggcaatcgt
     6061 gaaggtcacg tcacgattgc cctgccctac tatacgccta cgtactcagt acttagcggg
     6121 tgaccaccac cgcgtgcacg ccggccggaa ggtcggcgtc gggcgtgacg tagccgatcg
     6181 cgttcgggtg cgtcttcacg taatcgagca cggcctggtc gttcaggagc tcaggcggag
     6241 gcgtaacgtc ttcgcggtac gtcttggcgc gccagtacgc ctgaacggcc tggagcgggc
     6301 ggcccaggac ggcgaccgag aactcttccc ggacctgcgc ttcgggcatc tggtcgaccg
     6361 gggtcacggc ggagccgttc tgccaggtgg tccgcttggc gaggaacagg tcggcgacct
     6421 gccccgtgct ggcggtatag aactggttgt tgtcgttgac gacgatgaca caaccggcgg
     6481 tcttcgcggc cggctggaac atcatgagga ttgctacgag agccgaatac actgtcccct
     6541 ccctgaggtg tgcgctggcg cggccggatg gccgctccga tctgggcaga gtgtagcagg
     6601 gagggtgggc gggtctccaa taccgggtgc ggtggagtcc catgacggtt cggcatggct
     6661 ggcgggccag tgttattttg ggttgcgcgt gccggtagct cagttggtag agcaacggac
     6721 ttttaatccg taggtcctgg gttcgatccc cagccggcac attccttcct tatatcaggg
     6781 cgcctcgggg taggggacca gctgcccccg gggggcgaca aaccgtcgcg gcgccaccgg
     6841 cgccggctgc gagagtcgct ccagcaatgc gctggtcccc tcgatggatg ccgccgcccg
     6901 ccagacccac cccgaatccg acaccgagtc ccagcgggcc cctgagaacc agaaccgcac
     6961 gctgtccccc tccaccgcaa acgtcgagcg atacacgacg tccccgaact gcggaatgac
     7021 tccggcggtg atgaccggcg atgggaccac cgaccacgcc aggccgtccg ggctcgtcgc
     7081 caggcgcacc gcggtcggaa ggcagctctc gacgtacaac gcccaccact ccttgcggtc
     7141 gggaatccac tggacgtcga catgccatgg gtgacccgcg atcctggcag tgtcgggtac
     7201 ggaccaatgg acaccatccg tcgacgtccg gcgttgcagg aacgcggagc ccgccaccgc
     7261 gcagcctccc gcgccggcat ggacggacca gaggtaccag tccccgggtc cccgacggac
     7321 cacggcgggc gagatcatcc cgtgattcac ggctgaatcg atgtcgacgg gagaggtcca
     7381 attcaccccg tcccccgacc ggatcaattg caggacgttc cacatcggcg tcaccagccg
     7441 gacgtacatc cagatctgat tcgagtcagg tacgtagacg acgtccgggt cggtgtaggt
     7501 catggcggtc ggcggctgga gcaccgggtt gggcccgcct gccggcaggt gccagtgggt
     7561 acacgccgag tcctggctga acaaggacgg ccactcgcgg gtgggatcgg ctgccgggta
     7621 cgtcgtaatc gcgaggtatc ggggggatcc cggccatgac gcgggcatcc ggacgtagtc
     7681 cgggtgcact acgcgcccgg ttccgtctgc cgtggggatg gtgaggacgc gcaacgttcc
     7741 cctcacggac gtgtccggcg gaggggtcgt cggggcggga accggggcgg ccgtttcccg
     7801 gcacgccccg aggacgatga acagcaggcc gacggccagc gtgcggttca tggggtgcct
     7861 ggactcgaat cttcacgttt cgagggagtt gtcaaagaat ggaattgctg ccttgtgaaa
     7921 cctcccgccg ttgtcgaact ttgagcccgt atgccaacca ccatctggat caacggggaa
     7981 tacaagacca gggacgaagc gtccgtctcc gtcttcgacc acggcctcct gtatggggac
     8041 ggcgtttttg aggggatccg cgcctacggc gggcgtatct tccggctgag cgagcacatc
     8101 gaccggttgt attcctccgc caagtccatc tggctcgatc ccgggatgcc gcaggccgac
     8161 atgcggaaga tggtagaaga cgcgcaggcc aagagcggca tcaaggacag ctacatccgg
     8221 ttggtcatta cgcgcggtac cggggacctc ggcatggacc cccggaaatg cgacaaaccc
     8281 acggtcttct gcatcgtcga cacgatcgcg ctgtggtcca aggaacggta cgagcgcggg
     8341 ctgacctgca ttaccgcggc gactcccatc agccacaagg agaacctctc gccgcgcgtg
     8401 aagtccctca actacctgtc gcacatcctc gcgaaaatcg aaggcatcgc ggccaacgtc
     8461 gatgaagtca tcatgctcga cagctcgggg tacgtcgccg aggcgagcgg catgaacctg
     8521 ttttgcgccc gtcgcggcgt ggtgaagacc cctcccccgt actcagggat cctgagaggg
     8581 gtcacccgcg acgcgatgat cgagatggcg cgggacgcga attacgaagt agtggaagag
     8641 cccctcaacc ggtacgacct ctactcggcc gacgaggttt ttctgactgg cactgccgcc
     8701 gagatcgtcg ctgtcacgaa acttgacggt cgggtcatcg gaaatgggca gtcgggcccc
     8761 gtcgtcgctg atctgggccg gcgcttccgg gcgctggtga cccgaggtga ctgaccccgg
     8821 aacgcccttg ggactcccga aagggctcct cagggagccc tttttcgctc cccccatcag
     8881 gttatggctt ccataagcat ctcaactatt gacaggactt gacgttatgc gaatattgcg
     8941 cgccttgctt tctggtcacc cccgggaggt gagccttggc ctggcgcgta attgcccatg
     9001 gcgacgaggt ttggcatgtg gacgccttgg ctgagcgcag ggcccacacg gaggcgtggc
     9061 agctggtcct gacttttcgg gctgcctcgg aacggctccg ggcggcgtcg ttttgggcgc
     9121 cgtacccttt ggaagcgacg tccaagtcgt cgctgttcat ccaggcggag cggatacggg
     9181 accgggacct gtcgcgtctt ctggaggagc gtctctcatg aacgcggtcg acctgcacga
     9241 tcttcggcgg gcggcagcgg ccgtggcccg gcttcgggga gagacggtaa aggatgtgac
     9301 ggcacgttcg gacttacgcc agctcaaggt agagctggag tcgggattga tactggtggt
     9361 atcggcagag cgggacgaac agaaccggcc ccacctcgag gtggatgtgg tggaggcccc
     9421 gcaggcggcc agttcgcggc accagatcga agtgcggttc gactgatgtc cgccgatcag
     9481 ggtcccaacg ggggagctcc gcccgcgacg acggagcgcc tgcggttcca ggagttcacg
     9541 ttccagcggc tccc
//
LOCUS       DASUBP010000151         5525 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_154195, whole genome shotgun sequence.
ACCESSION   DASUBP010000151 DASUBP010000000
VERSION     DASUBP010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5525)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5525)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5525
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154195"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VFU75_05590"
     CDS             <1..574
                     /locus_tag="VFU75_05590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466341.1"
                     /translation="EKTKIAEAVPATRVAAAGKEKKSLMVPVAVGVVVVALGGGAWAV
                     FGPGHNAPPLATQHDTTTHQVAANPGRDTTQMSHADTTTKAPKNPGTHPANPANKSPG
                     PAALDSATLAGIAERLLDMSSRAIIDSGQLVFNGAHVSPLDRSTSACAVALAYQQGGR
                     RTEAVQWANKGLSIDPTLSSCKNVLNPGGQ"
     gene            575..1603
                     /locus_tag="VFU75_05595"
     CDS             575..1603
                     /locus_tag="VFU75_05595"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU5466342.1"
                     /translation="MFGRSPKPVKLHLFAKTDLGKTRDHNEDNYLVADLTRRVHELTP
                     SVKNHDVGERGSLLMIADGMGGAAAGEVASEMAADSVYEHLEHTWLADKEATAQRFAF
                     RLKEAIETANTKIHNYAKEHPEVRGMGTTTTAAGVLGDHLYLTQIGDSRAYLVRNGTA
                     IQITKDQSLMQRLVEAGELTEEEAAHSERRNIILQALGPDAKIKVDLTYQELRKGDTV
                     VLCSDGLSGQVKKEEIAEIAGKAVDLSAACDQLIALANERGGPDNITVIIARFDGDGL
                     RAASDGDAVGHQVYPLIDTENSTEPVPVYKGSRPPVPARQVRWRLVLGLVALAGLVLA
                     GIYIASRK"
     gene            1600..3006
                     /locus_tag="VFU75_05600"
     CDS             1600..3006
                     /locus_tag="VFU75_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEU5466343.1"
                     /translation="MKAQVKFLSGGRAGQVETISKTYIGIGRHPLSDIRFDAERDLDA
                     SSRHAAIMQRGSDYVLQDQQSKNGTFVNGKKIAGDVVLADGDVIKCGADGPAFEFHIL
                     ERDAQGTTPAEAAAARASAERVSIPAQRARSSTAVRVAMEVAKQTKSLRNTTKILFAL
                     LIVVAAGFGGLQWWSGHQRDLEIAALIARADSLQREELHFQTQVKGLQDAYNQAVGES
                     RRLQQDLADAKQNGDAATIARLRTQLDAAVDRQHNLSFAANVDYRAISNANQDAVALI
                     VVEYGDSSRFSGTAFAVDSQGDLITNRHVLLGDNDNQRPNRIAVKFSGSKQWFPAKYV
                     GSAPDADLGLIHVDIRGGTPRIAGISRDHDLQRGDPVAILGFPLGLDLPMEGTGLNAI
                     AAPTLSVGTASKVIPSLVQIDGYGAPGSSGSPIFNRDGKLVAVLYGGQAESGGRIIFS
                     VPAGTLLNYIASVNVKAP"
     gene            complement(3017..4147)
                     /locus_tag="VFU75_05605"
     CDS             complement(3017..4147)
                     /locus_tag="VFU75_05605"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF007148.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HEU5466344.1"
                     /translation="MTGTAGTLAVGLMSGTSLDGVSTALVRLNDAPASATLIAFRQEP
                     YNATERGQIIDAIARGSAKDLAILNVALGERFSGAVLHLLAEAKTSPKDIGFVASHGQ
                     TIWHEPGRATLQIGDPAIIAERVGVRVVSDFRARDVAAGGQGAPLVPLADVMLFGDDA
                     AGRLLLNIGGMANVTWVPRRGVTAGALAFDTGPGVAVVDAVTRTLDGEAAYDQDGERA
                     RRGRPASKVLEAFLADDYFRLRPPKSTGRERFGLAFAERLIAEVRGAGGSDNDAVATA
                     TALTAESIARAITEWTPAGPDADLVISGGGARNPTLVEWLAARVMPRRVVPFDSLFFD
                     GEAKEAVAFAYLGFLTLKGLPGNLPGATGARGSRVLGRITPP"
     gene            complement(4144..5073)
                     /gene="murQ"
                     /locus_tag="VFU75_05610"
     CDS             complement(4144..5073)
                     /gene="murQ"
                     /locus_tag="VFU75_05610"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016835 - carbon-oxygen lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEU5466345.1"
                     /translation="MTTPAFHDPRLTEQRNPRTQMIDVASSQEIVDLLNAEDATVAAA
                     VHSQRVSLARAIDLVLAAFQAGGRLLYVGAGTSGRLGVLDASECPPTFGSSPSQVVGI
                     IAGGLPALTKSAEGAEDDMNAGKAAVDGAGVDAKDVVIGIAASGTTPYVHAALERARE
                     RGARTGIVACSEPPKALRDACDVAIVPLVGPEALTGSTRMKAGTATKLVLNTISTGAM
                     IRLGKVYGNLMVDLMAWSEKLVDRGERIVMECGAPDRARARAAIEAAGGSVKVAVVMV
                     RAGVGADAARAKLMAAGGSVRKAAGDPPAIRKT"
     gene            5129..>5525
                     /locus_tag="VFU75_05615"
     CDS             5129..>5525
                     /locus_tag="VFU75_05615"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF013206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HEU5466346.1"
                     /translation="MRTRLLVALVLVAACGAFVKHADPTWGLTGKAVPVSGSHAMVVS
                     GHPLASAVGVQILKDGGNAIDAAVAVGFALEAVHPEAGNIGGGGFIVFRDSAGNARAL
                     DYRETAPAAATHNMYLDSAGNPTEASITGY"
BASE COUNT          944 a         1965 c         1807 g          809 t
ORIGIN      
        1 cgagaagacc aagatcgcgg aggcggtccc ggcgacgcgc gtggccgcgg cgggcaaaga
       61 gaagaagagc ctgatggtcc cggtcgcggt cggcgtggtg gtggtggcac tcggcggtgg
      121 cgcgtgggcc gttttcggcc caggccacaa cgcccccccg ctcgccaccc agcacgacac
      181 gacgactcat caagtagcgg cgaatccggg tcgcgacacg acccagatgt cgcatgcgga
      241 tacgacgacg aaggctccga agaatcccgg aacccacccg gcgaaccctg ccaacaagtc
      301 gcctggtccc gcggcgctcg attcggccac cttggccgga atcgcagagc ggttgctgga
      361 catgagctca cgagcgatta tcgactcagg tcagctcgtc ttcaatggtg cacacgtatc
      421 cccgctcgat cgatcgacgt cggcttgtgc ggtcgccctc gcttaccagc aaggcgggcg
      481 tcgcactgaa gccgtgcagt gggccaacaa gggcctttcc attgacccaa ccctgtcatc
      541 gtgcaagaac gtcctgaacc cgggtggaca atagatgttc ggacgctctc cgaagcccgt
      601 gaagctccac ctcttcgcca agaccgattt ggggaagacg cgtgaccaca acgaggacaa
      661 ctacctcgtc gcggatctga cgcgccgggt gcacgagctc acgcccagcg tcaagaacca
      721 cgacgtcggg gagcgcgggt cgctcctgat gatcgcggac ggcatgggcg gcgcagcagc
      781 gggcgaggtc gcgagcgaaa tggctgccga ctcggtctac gagcacctgg agcacacctg
      841 gctcgccgac aaggaagcca cggcccagcg cttcgcgttc cggctcaagg aagcgatcga
      901 gaccgcgaac acgaagattc acaactacgc caaggagcac cccgaggtcc gcggcatggg
      961 caccaccacc accgcggccg gcgtgctcgg tgaccacctc tacctcacgc agatcggcga
     1021 cagccgggcg tacctggtcc ggaacggaac cgcgatccag atcaccaagg atcagtccct
     1081 gatgcagcgc ctcgtcgagg ccggcgagct caccgaagag gaagcggccc actccgagcg
     1141 ccggaacatc atcctccagg cgctcggacc cgacgccaag atcaaggtgg atctgaccta
     1201 tcaggagctc cgcaagggcg acacggtggt gctctgttcg gatggactgt caggccaggt
     1261 gaagaaggaa gagatcgcgg agatcgccgg gaaggccgtc gatctctccg ccgcgtgcga
     1321 ccagctcatc gcgctggcca acgagcgcgg cggtccggac aatatcacgg tcatcatcgc
     1381 gcgtttcgac ggcgacggcc tccgggccgc ctccgacggc gacgccgtgg gtcaccaggt
     1441 atacccgctc atcgacacgg agaacagcac cgagccggtg cccgtgtaca agggaagccg
     1501 tccccccgtc cccgcccgtc aagtccgttg gcgcctcgtg ctcggcctgg tggcactggc
     1561 cggcctggtg ctcgcgggga tctacatcgc ctcccggaag tgaaggctca agtcaaattc
     1621 ctgtccggcg gccgcgccgg gcaggtcgaa acgatctcca agacctacat cgggatcggc
     1681 cgccaccccc tctccgacat ccgcttcgac gccgagcggg acctcgatgc ctcgtcccgc
     1741 catgcggcca tcatgcagcg cggctcggac tacgtcctcc aggaccagca gagcaagaac
     1801 ggtacgttcg tcaacgggaa gaagatcgcg ggtgacgtgg tcctcgccga cggcgacgtc
     1861 atcaagtgcg gcgccgacgg tccggccttc gagttccaca tcctggaacg cgacgcgcag
     1921 gggaccaccc cggctgaggc ggccgcggcg cgtgcctccg ccgagcgggt ttcgattccg
     1981 gcgcaacgcg cccgttcgag caccgcggtc cgggtggcga tggaggtggc gaagcagacc
     2041 aagtcgctgc gcaacacgac gaagatcctc tttgccctgc tcatcgtggt ggcggctggc
     2101 ttcgggggcc tgcagtggtg gagcgggcac cagcgcgacc tcgagatcgc ggcgctcatc
     2161 gcccgcgccg attccctgca acgggaagaa ctccacttcc agacgcaggt caaaggcctg
     2221 caggatgcct acaaccaggc cgtcggcgag tcgcgccgcc tccagcagga cctggcggat
     2281 gccaagcaga atggcgacgc cgcgacgatc gcgcgcctgc gcacccagct cgacgccgcc
     2341 gtggaccggc agcacaacct gtccttcgcc gcgaacgtcg actaccgggc catctcgaac
     2401 gcgaatcagg acgccgtggc gctcatcgtc gtcgaatacg gcgactcgag ccggttctca
     2461 ggcacggcct tcgccgtgga ttcgcagggc gacctgatca ccaaccggca cgtcctgctc
     2521 ggggacaacg acaaccagcg tcccaaccgg atcgcggtga agttctcggg ctccaagcag
     2581 tggtttcccg ccaagtacgt gggctcggca cctgacgcgg acctggggct gatccacgtg
     2641 gacatccgcg gcggcacgcc tcgcatcgcc ggcatctccc gcgaccacga cctccagcgc
     2701 ggcgacccgg tggcgatcct gggattcccg ctcggcctcg acctccccat ggaaggcacg
     2761 ggcttgaacg ccatcgccgc tcccacgctg tcggtgggaa cggcgagcaa ggtgatcccg
     2821 agtctcgtgc agatcgacgg ctatggagcg ccgggatcgt cgggcagccc gatcttcaat
     2881 cgggatggca agctggtagc ggtgctctac ggcggtcagg cggagtcggg cggcaggatc
     2941 atcttctcag tgccggcggg gacgctcctc aactacatcg cgtccgtgaa cgtgaaggcc
     3001 ccctagcggt cgatcatcac gggggagtga tgcggccgag caccctcgac ccgcgcgccc
     3061 cggtcgcacc cggcaagttc cccggcaacc ccttcagcgt caggaatccc aggtaggcga
     3121 atgccaccgc ctctttcgcc tcaccgtcga agaacagcga atcaaacggg acgactcggc
     3181 ggggcatcac gcgcgcggcc agccattcca ccagcgtggg atttcgagcc ccgcccccgg
     3241 agatcaccag gtccgcgtcg gggcccgcgg gagtccactc cgtgatggcc cgcgcgatcg
     3301 actccgcggt gagcgccgtc gccgtcgcta cggcgtcgtt gtccgagccc cccgccccgc
     3361 gaacctcggc gataaggcgt tccgcgaacg ccaatccgaa tcgttcgcgc cccgtgctct
     3421 tggggggacg cagccggaag tagtcatcgg ccaggaacgc ctcgagcacc ttcgacgccg
     3481 gacgcccgcg gcgcgcgcgc tcgccgtcct gatcgtaggc cgcctcccca tccagtgtcc
     3541 tggtcacggc gtcgaccacc gccacgccgg gaccggtgtc gaacgccagg gcgccggcgg
     3601 tgacgccgcg ccggggcacc caggtgacgt tcgccatgcc accgatgttg agcagtaacc
     3661 ggccagccgc gtcgtccccg aacagcatga cgtccgcgag gggtacgagg ggcgcgccct
     3721 gcccccccgc cgccacatcg cgggcgcgga agtcggagac cacccgcacg ccaacccgct
     3781 ccgcgatgat ggccggatcg ccgatctgca gggtggcgcg gccgggctcg tgccagatgg
     3841 tctgcccgtg gctcgcgacg aacccgatgt cctttggaga ggtcttggct tccgcgagca
     3901 ggtggagcac cgcgccggag aagcgctcgc ccagcgcgac gttgaggatg gcgaggtctt
     3961 tcgccgaacc gcgggcgatc gcgtcgatga tctgaccccg ctcggtcgcg ttgtacggtt
     4021 cctggcggaa ggcgatcagc gtggcggaag cgggcgcatc attgaggcga accagcgccg
     4081 tcgacacgcc gtcgagagac gtgcccgaca tcagaccgac ggcgagcgtc ccagccgtcc
     4141 cggtcacgtc ttgcggattg cgggaggatc tccggcggct ttgcgaaccg agcccccggc
     4201 agccatgagc ttggcgcggg ccgcgtcggc accgacgccc gccctgacca tcacgacggc
     4261 gaccttcacg cttccgcccg ccgcctcgat cgccgcgcgg gcccgggcgc ggtcgggggc
     4321 gccgcactcc atcacgatgc gctccccccg gtccaccaac ttctcgctcc aggccatcag
     4381 gtccaccatc aggtttccgt agaccttgcc gagacggatc atcgcgccgg tggagatcgt
     4441 attgagcaca agcttggtcg cggtccccgc tttcatccgg gtggagccgg tcagtgcctc
     4501 agggcccacc agggggacga tcgcgacatc gcaggcgtcg cgaagcgcct tcgggggctc
     4561 gctgcaggcc acgatacccg ttcgggcgcc ccgttcccgg gcgcgctcca gcgcggcatg
     4621 cacgtagggc gtcgtcccgc tcgcggcgat gccgatgacg acgtccttcg cgtcgacccc
     4681 ggcgccatcg acggccgcct tccccgcgtt catgtcatcc tcggctccct cggcactctt
     4741 cgtcagcgcg ggtaaccccc ccgcgatgat gccgaccacc tgtgacggag aggacccgaa
     4801 cgtcggcggg cattcggagg catcgagaac cccaaggcgc ccggaagtcc ccgcaccgac
     4861 gtacaggaga cgtccacccg cctggaacgc cgcgaggacc agatcgattg cgcgagccag
     4921 gctcacgcgc tggctgtgca cggctgccgc cacggtcgcg tcctcggcgt tcaggaggtc
     4981 cacgatctcc tgactggagg ccacatcgat catctgggtg cgaggattgc gctgctcggt
     5041 gaggcggggg tcgtggaagg cgggagtcgt cattgcggtc cccaagttag acgttgaccg
     5101 cgcgcacggt caacgtgcag tatagggaat gcgcacacgt cttctcgttg cactcgttct
     5161 ggtcgccgct tgcggcgcct tcgtcaaaca cgcggacccc acgtggggac tcaccggcaa
     5221 agccgttccc gtgtccggca gccacgcgat ggtcgtgtcc gggcaccccc tggcctccgc
     5281 cgtcggcgtg cagatcctga aggatggcgg caacgccatc gacgccgcgg tggcggtcgg
     5341 tttcgccctc gaggccgtcc atcccgaggc cgggaacatc ggcggcggcg gattcatcgt
     5401 cttccgcgac tcggccggca atgcccgcgc gctggattac cgggagaccg cccctgccgc
     5461 cgccacgcac aacatgtatc tcgattcggc cgggaacccc accgaagcca gcatcacggg
     5521 atacc
//
LOCUS       DASUBP010000152         6624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_944580, whole genome shotgun sequence.
ACCESSION   DASUBP010000152 DASUBP010000000
VERSION     DASUBP010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6624
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_944580"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2228)
                     /gene="rpoB"
                     /locus_tag="VFU75_05620"
     CDS             complement(<1..2228)
                     /gene="rpoB"
                     /locus_tag="VFU75_05620"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEU5466347.1"
                     /translation="MTNNKTLPVLSFAKLEEGMSMPHLLDIQTRAFQSLLQPDGKGDA
                     RADVGLERVFKDLFPIQDVNGNYSLEFVHYALGEPKYSVEECIERDMTYSVPLKATLQ
                     LVVMEEVGETTKTKRPKNIIEKEVYLGELPILTPLGTFVINGAERVIVSQLHRSPGVV
                     FEESTHPNGQRLFSARIIPFRGSWVEFTVDIHDVIYVHIDKKKKFPATALLRAFGYGS
                     NADILKLFYATKELDITGKLEGRAQKREVLGTLLSTDVPDPENKKGEPLARVGDELTL
                     DRFNAFRRAGITKVTVFAGYTSLDLRDDAQPTTTRDREGKYILAFDVADPETGEVVAE
                     GGREISDTMKKKLLKAGLTKVDVLLPPGRSESPLIKNTLLKDPTHSEAEALEQIYTLL
                     RPGEAPNLETARQALERLFFHPKRYDLGRVGRYKINQRLKVGAAKDQTVLTEEDFVAI
                     IRYLIELSEGRGYTDDIDHLGNRRVRSVGELIANQFSVGLSRMARLIKERMSINNDPE
                     RITLDDLVNARTVSAVIQAFFGSSQLSQFMDQTNPLAELTHKRRLSALGPGGLTRERA
                     GFEVRDVHYSQYGRMCPIETPEGPNIGLITSLSCYARVNDLGFIETPYRVVENGRVTG
                     QIRWLSASEEEEYAVAQANAKLTEDGRFADELVLCRKRDDYPLLAPGRIDFMDVAPEQ
                     LVSIAAALIPFLEHDDANRALMGSNMQRQSVPLLFPQAPVVGTGLEEKVARDSGAVVI
                     ARR"
     gene            complement(2408..2797)
                     /gene="rplL"
                     /locus_tag="VFU75_05625"
     CDS             complement(2408..2797)
                     /gene="rplL"
                     /locus_tag="VFU75_05625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEU5466348.1"
                     /translation="MTTQTQLSKDDIISAIGNMSVFELAELIEAFKEKFGVTIAAPVA
                     AAAPAGAAAAAPAAEEKTEFTVVLKSGGDKKIQVIKEVRTITSLGLKEAKDLVEAGGT
                     VKEGVSKQEADEIKKKLEAQGATVELK"
     gene            complement(2817..3353)
                     /gene="rplJ"
                     /locus_tag="VFU75_05630"
     CDS             complement(2817..3353)
                     /gene="rplJ"
                     /locus_tag="VFU75_05630"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEU5466349.1"
                     /translation="MSATKTEKQESVTTLTAKLKQSPSLYLTDFTGLNVARITDLRRR
                     LRKAGGEYVVVKNTLARRALGDAKVPGLDEHLAGPTALVLAGSDPVASAKVLTDFAKE
                     FEKPSVKIGLVDGKAVTPEQVKRLAALPSKTELLSQLGGALQAPMAGFVGALNGLLMN
                     MVGALEALKAKRSSGAAS"
     gene            complement(3350..4039)
                     /gene="rplA"
                     /locus_tag="VFU75_05635"
     CDS             complement(3350..4039)
                     /gene="rplA"
                     /locus_tag="VFU75_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEU5466350.1"
                     /translation="MRVHGKKFRAAVGQLEPRKLYPAKEAVEKVKSTAFAKFDESVDI
                     SVRLGVDPKHADQIVRGTVSLPHGTGKSVRVLVIAQGDKAKEAEAAGADAVGVEYLQK
                     IKDGWLDTDVIVATPDVMGQLGPLGKVLGPRGLMPNPKAGTVTMDVTKAVKELKAGKI
                     EFRVDKAGIVHAPIGKRSFQVNALEENLHAFMDAIVRAKPAAAKGHYVRSVTVSSTMG
                     PGIHLDPAVYA"
     gene            complement(4039..4464)
                     /gene="rplK"
                     /locus_tag="VFU75_05640"
     CDS             complement(4039..4464)
                     /gene="rplK"
                     /locus_tag="VFU75_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006676477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEU5466351.1"
                     /translation="MAKPITAVVKLQVPAGQANPAPPVGTALGPHGVNIMDFVKQFNA
                     KTQSAMGMITPVEVTIYKDRTFTFITKTPPAAVLLKKEAGIEKGSAQSNRNKVGTVTK
                     AQVKKIAELKMPDLNATSLESAMKMVAGTARSMGLEVKD"
     gene            complement(4527..5072)
                     /gene="nusG"
                     /locus_tag="VFU75_05645"
     CDS             complement(4527..5072)
                     /gene="nusG"
                     /locus_tag="VFU75_05645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEU5466352.1"
                     /translation="MADRRWYAVQTTAGHENKVRSLIERRIQDDPRSDEEKPIKLAMV
                     PTQEVVEIKNGKKETVKRKIYPGYVLVEMALNQETSHVINGIQGVIKFVGTGKEPMAL
                     RSEEVNRLLGIAEPVQEEEPVQEIPFLVGQVVEIIEGPFSDFSGTVEEVIPDKGKVRV
                     SVSLFGRPTSVELDYLQLKAH"
     gene            complement(5072..5311)
                     /gene="secE"
                     /locus_tag="VFU75_05650"
     CDS             complement(5072..5311)
                     /gene="secE"
                     /locus_tag="VFU75_05650"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEU5466353.1"
                     /translation="MATDAVTSPEGGRPGFLSRLVSFLREVQAETKRVTWPSWIELRK
                     ATVAIIAFTLVLGIVIGLMDTLLQFLLVTAVAKVF"
     gene            complement(5314..5466)
                     /gene="rpmG"
                     /locus_tag="VFU75_05655"
     CDS             complement(5314..5466)
                     /gene="rpmG"
                     /locus_tag="VFU75_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEU5466354.1"
                     /translation="MPRENIIMECTTCKNRNYFTTKNRRKHPERAEWKKYCPRCNKPQ
                     VHKESK"
     gene            complement(5566..>6624)
                     /gene="tuf"
                     /locus_tag="VFU75_05660"
     CDS             complement(5566..>6624)
                     /gene="tuf"
                     /locus_tag="VFU75_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEU5466355.1"
                     /translation="DTVAKASESQGRRDATKILTIATSHVEYSTDERHYAHVDCPGHA
                     DYVKNMITGAAQMDGAILVVSAVDGPMPQTREHILLARQVNVPAIVVFLNKCDLIDDP
                     ELLDLVELEVRELLSKYEFPGDEIPVIRGAALKAIEGDAKYVEKVWELLKALDKNIPI
                     PVREVDKPFLMPVEDVFSITGRGTVATGRIERGKIKVGEEVEMVGFGTDKKTVVTGVE
                     MFRKLLDEGQAGDNAGLLLRGIEKKDIERGMVLAKAGSITPHTAFDAEVYVLTKDEGG
                     RHTPFFKGYRPQFYFRTTDVTGTVELPAGVEMVMPGDNTKMAIELITPIAMEEGLRFA
                     IREGGRTVGAGVVTKIRK"
BASE COUNT          988 a         2268 c         2054 g         1314 t
ORIGIN      
        1 cggcgcgcga tgaccacggc gcccgaatcc cgggccacct tctcctcgag cccggtgccc
       61 acgacggggg cctgcgggaa gaggagcggc accgactgcc gctgcatgtt cgagcccatg
      121 agcgcgcggt tcgcgtcgtc gtgctcgagg aacgggatca gggccgcggc gatcgagacc
      181 agctgctcgg gcgccacgtc catgaagtcg atccgccccg gcgcgaggag cgggtagtcg
      241 tcccgcttcc ggcagagcac cagctcgtcc gcgaagcggc cgtcttcggt cagcttcgcg
      301 ttggcctgcg ccaccgcgta ttcctcttct tcactcgccg agagccaccg gatctgcccg
      361 gtgacgcgcc cattctccac cacgcggtac ggcgtttcga tgaagccgag atcgttcacg
      421 cgggcgtagc acgagaggct cgtgatgagc ccgatgttcg gaccttccgg cgtctcgatc
      481 ggacacatcc ggccgtactg gctgtagtgc acgtcgcgga cttcgaagcc cgcgcgctcg
      541 cgcgtcaggc cgccgggtcc cagggcgctc agcctccgct tgtgcgtcag ctcggcgagc
      601 gggttggtct ggtccatgaa ctgcgacagc tgcgacgatc cgaagaacgc ctggatgacg
      661 gcgctcacgg tccgggcgtt caccaggtcg tccagggtga tccgctcggg gtcattgttg
      721 atgctcatcc gttccttgat gagccgcgcc atgcgcgaga gacccaccga gaactggttc
      781 gcgatgagct cgcccaccga gcgcacccgg cggttgccca ggtggtcgat gtcgtccgtg
      841 tacccgcggc cctcggagag ctcgatcagg tagcggatga tggcgacgaa atcctcttcg
      901 gtgaggacgg tctgatcctt cgccgcgccc accttgagcc gctgattgat cttgtaccgg
      961 cccacccgtc ccaggtcgta gcgcttggga tggaagaaca gccgctcgag cgcctggcgg
     1021 gcggtctcga ggttcggcgc ttcgccgggc ctgagcagcg tgtagatctg ctccaacgcc
     1081 tcggcctcgg agtgcgtcgg gtccttgagc agggtgttct tgatcagggg cgactcactc
     1141 cggcccggag gcagcagcac gtcgaccttg gtcaacccgg ccttcaggag cttcttcttc
     1201 atcgtatcgg agatttcgcg gccgccttcc gccacgactt cgccggtctc cggatccgcc
     1261 acgtcgaacg cgaggatgta cttgccctcg cggtcacgcg tggtggtggg ctgggcgtca
     1321 tcccgcaggt cgagcgacgt gtacccggcg aacaccgtca ccttggtgat cccggcgcgg
     1381 cggaacgcgt tgaaccggtc gagggtgagc tcgtcgccca cacgggcgag cggctcgccc
     1441 ttcttgttct ccggatccgg cacgtcggtg gacaggaggg tgccgaggac ttcgcgcttc
     1501 tgggcgcgac cctcgagctt gcccgtgatg tccagctcct tggtcgcgta gaacagcttg
     1561 aggatgtccg cgttggagcc gtagccgaac gcgcgcaaca gcgcggtggc cgggaacttc
     1621 ttcttcttgt cgatgtggac gtagatcacg tcgtggatgt cgaccgtgaa ctccacccac
     1681 gagccgcgga acggaatgat ccgggccgag aacaggcgct ggccgttggg gtgcgtgctc
     1741 tcttcgaaca cgacgcccgg cgaccggtgc agctgcgaca cgatcacgcg ctcggcgccg
     1801 ttgatgacga aggtgccgag gggcgtgagg atgggcagct caccgagata gacttccttc
     1861 tcgatgatgt tcttcgggcg cttggtcttg gtggtctccc ccacctcttc catgacgacg
     1921 agctgcaggg tggccttgag gggcaccgag tacgtcatgt cgcgctcgat gcactcttcg
     1981 accgagtatt tcggctcgcc gagggcatag tgcacgaact cgagggagta gttcccgttc
     2041 acgtcctgga tcgggaacag gtccttgaac acgcgttcca agccgacgtc cgcgcgcgcg
     2101 tcgcctttcc cgtccggctg caggagggac tggaacgcgc gggtctgaat gtcgaggagg
     2161 tggggcatgg acatgccctc ctcgagcttg gcgaacgaca gcaccggcaa ggtcttgttg
     2221 ttggtcatgt cacgtcctta aagacaacgc aaaaggccca gcgccccccg gaccggtggc
     2281 tggtccaggg gtcgcgatgg gtgctcaaac cgagcaggag tgtagcgatt tcgtgcatgc
     2341 cgtccaaccg aaggagacca gggcgcgcca acggacccct gtggtccgcg gcgtccaacc
     2401 cgacggtcta cttcagttcg acggtggcgc cctgcgcttc gagcttcttc ttgatctcgt
     2461 cggcttcctg cttggacacg ccttccttga cggtcccgcc ggcctcgacc aggtccttgg
     2521 cttccttgag gcccagactg gtgatcgtcc ggacttcctt gatgacctgg atcttcttgt
     2581 cgccgccgct cttgaggacg accgtgaact cggtcttctc ctcagccgcg ggcgccgccg
     2641 ccgccgcgcc ggccggcgcc gccgccgcca cgggggcggc gatggtgacg ccgaacttct
     2701 ccttgaaggc ttcgatcagc tcggcgagct cgaacaccga catgttgccg atcgccgaga
     2761 tgatgtcgtc cttgctgagc tgtgtctgcg tggtcatttg agtgtctccc tgccggttaa
     2821 gaagcagcgc ccgaggagcg cttggccttg agcgcctcga gggcacccac catgttcatc
     2881 aacagtccgt tcaacgcacc gacgaatccc gccatcggcg cctgcagcgc gccgcccagc
     2941 tgactgagga gctcagtctt cgaaggcagc gccgccagcc gcttcacctg ctccggcgtc
     3001 acggccttgc cgtcgacgag cccgatcttg acggacggct tctcgaattc cttcgcgaag
     3061 tcggtcaaca ccttggccga cgcgaccgga tccgacccgg ccagcaccag cgccgtcgga
     3121 cccgcgagat gctcgtccag ccccggcacc ttggcatcgc cgagggcgcg ccgggccagg
     3181 gtgttcttca cgacgacgta ttccccgccc gccttgcgaa gccggcggcg gaggtccgtg
     3241 atccgggcga cgttcagccc ggtaaagtcc gtgaggtaca gggacggcga ctgcttcagc
     3301 ttggccgtca gcgtcgtcac ggattcctgt ttttcggtct tggtcgcgct catgcgtaca
     3361 ccgccgggtc cagatggatg ccggggccca tggtgctcga caccgtcacc gagcgcacat
     3421 agtggccctt ggccgccgcg ggcttggccc gcacgatcgc gtccatgaac gcgtgcagat
     3481 tttcctccag ggcgttcacc tggaaggacc gcttcccgat gggggcgtgc acgatccccg
     3541 ccttgtccac ccggaattcg atcttgcccg ccttcaattc cttcaccgcc ttggtcacgt
     3601 ccatggtgac cgtgcccgcc ttcgggttcg gcatcaggcc ccgggggccc agcaccttgc
     3661 cgaggggtcc cagctgtccc atcacgtccg gcgtggccac gatgacgtcg gtatcgagcc
     3721 agccgtcctt gatcttctgg aggtactcga cgccgaccgc gtccgcgccg gccgcttccg
     3781 cttccttcgc cttgtcgccc tgtgcgatca ccaacacccg gacggacttc ccggttccgt
     3841 gcgggagcga cacggtgccg cggacgatct gatcggcgtg cttgggatcg acgcccaggc
     3901 gcaccgagat gtcgacggac tcgtcgaact tcgcgaacgc ggtggacttc accttctcca
     3961 ccgcttcctt ggccggatac agcttgcgcg gctcgagctg gccgacggcg gcccggaact
     4021 tcttcccgtg gacgcgcatc agtccttcac ctcgagcccc atcgagcgcg cggtgcccgc
     4081 caccatcttc atggcggact cgaggctcgt ggcgttcagg tcgggcatct tgagctcggc
     4141 gatcttcttc acctgggcct tcgtcaccgt accgaccttg ttccggttgg actgggcgga
     4201 gcccttctcg atgccggcct ctttcttcag gaggacggcc gcggggggcg tcttggtgat
     4261 gaaggtgaag gtccggtctt tgtagatcgt gacttcgacc ggcgtgatca tgcccatcgc
     4321 gctctgggtc ttcgcgttga actgcttcac gaagtccatg atgttcacgc catggggtcc
     4381 caacgccgta ccgaccggcg gcgccgggtt cgcctgcccg gcagggacct gcagtttcac
     4441 cactgcggtg attggtttgg ccatcgtctt cctctctata gcgtcagccg actatgcggc
     4501 cgactcccac ttggcccgtg gtagggctaa tgcgccttca actgtagata atccagttcc
     4561 acggacgtcg gtctgccgaa caacgagacc gacacccgga ccttgccctt gtccgggatc
     4621 acctcttcca ccgttcccga gaaatccgag aacggaccct cgatgatctc gaccacctgc
     4681 ccgaccagga acgggatctc ctgcaccggc tcttcttcct gtaccggctc ggcgatgccc
     4741 agcaggcggt tcacttcctc gctgcgcagg gccatcggct ccttgccggt gccgacgaac
     4801 ttgatcacgc cctgaatccc gttgatgacg tggctggtct cctgattcag cgccatctcg
     4861 accagcacgt acccgggata gatcttccgc ttgaccgttt ccttcttgcc gttcttgatc
     4921 tccaccactt cctgggtggg gaccatcgcg agcttgatcg gcttctcctc gtcggaccgc
     4981 ggatcgtcct ggatccggcg ctcgatgagg gagcggacct tgttctcgtg cccggccgtg
     5041 gtctgcacgg cgtaccagcg gcggtccgcc atcaaaagac cttggccacg gcggtcacca
     5101 gcaggaactg cagcagcgtg tccatgagtc cgatcacgat gcccagcacc agcgtgaacg
     5161 cgatgatggc gaccgtcgcc ttccgcagct cgatccacga cggccaggtg acccgcttgg
     5221 tctcggcctg gacctcacgc agaaacgaca cgagccggga gagaaagccc ggcctcccgc
     5281 cttccggcga ggtgaccgcg tccgtcgcca cggctacttc gattccttgt ggacctgcgg
     5341 cttgttgcac cgcgggcagt acttcttcca ctccgcgcgc tcagggtgct tccgccggtt
     5401 cttcgtcgtg aagtagttcc ggtttttgca cgtcgtgcat tccataatga tgttttcgcg
     5461 gggcatttat ccctcagatt tcggatttcg gattgcggat tgcggattca tcggatgtcc
     5521 cggcttcagg cactcaatcc gcaatccgca ctccgcaatc ggcaatcact ttctgatttt
     5581 cgtgaccacg cccgcgccga ccgtccggcc accttcccgg atcgcgaacc gcaggccctc
     5641 ttccatcgcg atcggcgtga tcagctcgat cgccatcttc gtgttgtccc ccggcatcac
     5701 catttcgacg ccggccggca actccaccgt ccccgtcacg tccgtcgtcc ggaagtagaa
     5761 ctggggccgg tagcccttga agaacggcgt gtgccgcccg ccctcgtcct tcgtcaggac
     5821 gtagacctcg gcgtcaaagg ccgtgtgcgg cgtgatcgag cccgccttcg ccagcaccat
     5881 cccgcgctcg atgtccttct tctcgatgcc gcggagcagg aggccggcgt tgtcgcccgc
     5941 ctggccctcg tccaggagct tccggaacat ctcgaccccg gtcacgaccg tcttcttgtc
     6001 ggtcccgaag cccaccatct ccacttcctc gccgaccttg atcttgccgc gctcgatccg
     6061 gcccgtggcc accgtgcccc ggcccgtgat cgagaacacg tcctcgacgg gcatcaggaa
     6121 cggcttgtcg acctcgcgca ccgggatcgg gatgttcttg tcgagggcct tgaggagctc
     6181 ccagaccttc tcgacgtact tcgcatcgcc ctcgatcgcc ttcagggcgg cgccccggat
     6241 caccgggatc tcgtcgcccg ggaactcata cttggacagg agctcccgca cctcgagctc
     6301 caccagatcc aggagctcgg ggtcgtcgat cagatcgcac ttattgagga agacgacgat
     6361 cgccggcacg ttcacctgcc gggcgagcag gatgtgctcg cgcgtctgcg gcatcgggcc
     6421 gtcgacggcc gagaccacga ggatcgcgcc gtccatctgc gcggcccccg tgatcatgtt
     6481 cttcacgtag tcggcgtgcc ccgggcagtc gacgtgcgcg tagtggcgct cgtccgtcga
     6541 atactccacg tggctcgtcg caatcgtcag aatcttggtc gcgtcgcggc gcccctggga
     6601 ctccgacgcc ttggcgacgg tgtc
//
LOCUS       DASUBP010000153         6697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_385989, whole genome shotgun sequence.
ACCESSION   DASUBP010000153 DASUBP010000000
VERSION     DASUBP010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6697
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385989"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1394)
                     /locus_tag="VFU75_05665"
     CDS             complement(<1..1394)
                     /locus_tag="VFU75_05665"
                     /inference="COORDINATES: protein motif:HMM:NF012612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEU5466356.1"
                     /translation="MLPPAMNAFRILVFSAAMQTPQASPDTLRAPAVMKNLSHVPHVV
                     EVDLTAAPARLALYPGKVTDVWAYNGQVPGPTLDVNEGDSVIVHFTNDLPEATTIHWH
                     GMHLAAEMDGSPIYAVPPGGRTDYVFKVGLDRAGTYWYHPHPDKRSGHQVEMGLYGGL
                     IVHAAHDPLPPIPEKLLILSDNRFNPDGSVDFPDPNSLAGQIDVENGREGPDLFINGQ
                     LDPVIPIRSGEIQRWRIINASAARVYRLSIPGQSILWVGSDGGLFEHPEDVHDVLIAN
                     SERVEILVRGNGAPGSKASFVTLPYDRYDPHTRPANWDETHELARLAYSTLPPVTPAA
                     IPDVVRPVPAIDTAQVAATKVVVFTQGLVNGRTMDMSRVDFRSRVGTTEIWEIDNLVA
                     MDHPFHLHGFRFQILDRNGVPEPHRRWKDTVNVPKHQSVRIIVHFADFAGKWMFHCHI
                     LEHEDEGMMGILQT"
     gene            1734..1946
                     /locus_tag="VFU75_05670"
     CDS             1734..1946
                     /locus_tag="VFU75_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466357.1"
                     /translation="MTAIANDKLVLEDDLDVGHLVIITNRPSGARTLRRLQPSEADTY
                     LGDVLRIEAGEGPGERRLRVVRPAGR"
     gene            2059..2541
                     /locus_tag="VFU75_05675"
     CDS             2059..2541
                     /locus_tag="VFU75_05675"
                     /inference="COORDINATES: protein motif:HMM:NF042549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6632 domain-containing protein"
                     /protein_id="HEU5466358.1"
                     /translation="MKPAAEHYVHLGIRTMEDGMKRQRMAQVALILVGLLYLSWFIPL
                     LQALWQSSWIQGNQEAFPMFTTVNTVLGVFLLLAVRRPAQHRLLIAFGAWSSLAHAST
                     MAIMTAEARAHGIPRSPLDVFVIGAAGVVLLTVLAALREKHPSPSGPAPISAPRFAEG
                     "
     gene            complement(2559..3239)
                     /locus_tag="VFU75_05680"
     CDS             complement(2559..3239)
                     /locus_tag="VFU75_05680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466359.1"
                     /translation="MTFALFLILAQASRPLLPLRIPSAALPQTMEFAVSRVTDSGVGA
                     RVATWHLEYRAVEIRGAKAVEYVSRFSGLNGKTLTVDSVTLMVDGMVPVSERSHQSTK
                     IMNLRFDRDSTIADVTDSGGAHHVVTPTPPPYFNSTDTPLAILSLPAERGIRGVIREY
                     QYEAGGLVDDTLSVEDVAPGRMTARSATAEGIWTWEIDTRAHTILHASRRGRAGGAGI
                     DFSRTDHR"
     gene            3419..4519
                     /locus_tag="VFU75_05685"
     CDS             3419..4519
                     /locus_tag="VFU75_05685"
                     /inference="COORDINATES: protein motif:HMM:NF040149.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5700 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HEU5466360.1"
                     /translation="MRRPSVILSLSLLILATVRSAAQISDPLPATGISFAGVDEFWSI
                     GGILSHDSEPTAAQWQALFSTPGYRLALKNLGPGLKDDIDLALRPSRHAEFVRVSSGT
                     DDHSLMVEHLAQAWSRRAVLRAYRDSLARGTPVADARALASRFLPPGATRNGPPPLVT
                     FAFFKDDGYSFPEGIVVDLLYARSEAAGGTPLVRNLAHEFHHSYVNRMARPASPGATP
                     AEGALRDALYALRNEGLADLIDKPYPFRAPVPGLAAYAVRYDTEYARTPATLRTLDSL
                     LAAIAGDSGASADAAMQASMLFWSNGHPNGAYVAREILETFGVDSLYPAARSPAAFLR
                     TFRWAERKHGRPDPFSDAARRELDSLDVRYWR"
     gene            4716..5390
                     /locus_tag="VFU75_05690"
     CDS             4716..5390
                     /locus_tag="VFU75_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466361.1"
                     /translation="MPAAVGAQAAPASPTPSAPYPTTMAPVDQYRMDRSAEIALAKSA
                     APASISDSADVMVLGASGYETAVKGKNGFVCIVGRAWSRDFGLDDFWSPNTRAPVCFN
                     AAAVRSVLPWYLIRTKWALSGMTEAQMEARARKSPVAAGVRAPEPGAVGFMQSKEGYI
                     NEDVKGPWHPHVMFYAPLTKPLTDPTAWGANLHGSPLHVNDAETAYYTTFAVPVTQWS
                     DGTPDK"
     gene            complement(5460..6335)
                     /locus_tag="VFU75_05695"
     CDS             complement(5460..6335)
                     /locus_tag="VFU75_05695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEU5466362.1"
                     /translation="MDVPLLIRARLATLGLEQRDLARAAQVTESYISQLLKRKKAPPA
                     PGRTDIYGRMDKFLQLPRGELARLAAFQRREQLKRTLGDEAVPVPLFPDLRALILNKC
                     DPAKAAAVRAIFEKQPFGELERLVAQKLLDVTKRAAKEELENEKWTRGVARRAGRSYE
                     RTRVRTLEFLDTDIFHLSIRDGEAFLGPLIESWDIDLVTFGLKVILNRRLTTVPDQRF
                     EFTEQPLEGPAEEEPGFRAFLNDPTMSGTATEGELTWLKRLPTDGRHPTALFYYRALQ
                     ALRDPLNFTGRVRPG"
     gene            6497..>6697
                     /locus_tag="VFU75_05700"
     CDS             6497..>6697
                     /locus_tag="VFU75_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466363.1"
                     /translation="MFARNVTLHLKPNTAPEFTRTVETDVLPVMRKQSGFKDEITFIE
                     RQGTEAVAISLWENKENAEAYGR"
BASE COUNT         1159 a         2213 c         2134 g         1191 t
ORIGIN      
        1 taggtctgga gaatccccat catgccttcg tcctcgtgct cgaggatgtg acaatggaac
       61 atccacttcc ctgcgaagtc ggcgaagtgg acgatgatgc gtaccgactg gtgcttgggc
      121 acgttgaccg tgtccttcca gcgacggtgc ggctccggca ccccgttgcg atcgaggatc
      181 tggaaccgga atccgtgcag gtggaatggg tgatccatcg ccaccaggtt gtcgatctcc
      241 cagatctccg tcgtgccgac gcgtgaccgg aaatccacgc ggctcatgtc catcgtgcgg
      301 ccgtttacca ggccctgcgt gaacaccacc accttggtcg ccgccacttg ggcggtgtcg
      361 atggcgggga ccggccggac cacatccggg atcgcggccg gggtaaccgg aggcagcgtc
      421 gagtacgcga ggcgggcaag ctcgtgggtc tcgtcccagt tcgcgggccg ggtgtgcggg
      481 tcataccggt cgtatggcag ggtgacgaac gaggccttcg accccggtgc tccgttgccc
      541 cggaccagga tctcgacgcg ctcggagttg gcgatcagga cgtcgtgcac gtcttcgggg
      601 tgctcgaaca ggccgccgtc cgaccctacc cacagaatgc tttgacccgg tatcgagagc
      661 cggtaaacgc gcgcggccga agcgttgatg atccgccagc gctggatttc cccgcttcgg
      721 atgggaatca ccgggtcgag ctggccgttg atgaacaggt ccgggccttc ccggccgttc
      781 tccacgtcga tttgtcccgc cagtgaattg gggtcgggga agtcgacgga accgtcgggg
      841 ttgaaccggt tgtccgagag gatcaagagc ttttcgggaa tcggcggcag cgggtcatgg
      901 gcggcgtgga cgatcaggcc cccatacaga cccatctcca cctgatggcc cgaccgtttg
      961 tccgggtgcg ggtggtacca gtaggtgccc gcgcggtcca atccgacttt gaagacgtaa
     1021 tcggtccggc cccccggggg gaccgcgtag atcgggctcc cgtccatctc cgcggcgagg
     1081 tgcatcccgt gccaatggat ggtcgtcgct tcggggaggt cgttcgtgaa gtgcacgatc
     1141 acgctgtccc cctcgttgac gtccagcgtg gggcccggca cctggccgtt gtaggcccag
     1201 acatccgtga ccttcccggg ataaagcgcc agtcgcgccg gcgcggcggt caggtcgact
     1261 tcgaccacgt ggggaacgtg tgagagattc ttcatcaccg cgggggcgcg gagcgtgtcc
     1321 ggcgaggcct ggggtgtctg catggcggcg gaaaagacga ggatccggaa agcgttcatc
     1381 gcaggtggaa gcaccgttgg gacctcctat gatctcgaag atctcgagca acgcctcagc
     1441 cgccgatgga accgcgttaa cgcgggtgcc aaggtgcagc cccgcttcac gtctggctag
     1501 tgcgggcatt gggcttttca ggcactcccc cggttcaatc ccagggattc tgtgaagggc
     1561 ttctgaagac gtgattacaa agagcattcc acagtgcggt tcgcggcggg ccgtgcgcct
     1621 tgcggcagca ggagaaggcc actggccgcg tcacagcgcg tcgccgtgtg gcccgtcctt
     1681 tgcaccccca agaccgggga cggcgatacc agtcacgaga ccacggagga cgcatgaccg
     1741 ccattgccaa cgacaagctg gtgctggaag atgacctcga cgtcggccac ctggtcatca
     1801 tcacgaatcg ccccagtggt gccaggacct tgcgtcggct gcaaccctcg gaagccgata
     1861 cctacttggg cgacgtgttg cgcatcgagg ccggcgaggg ccccggtgag cgccggctgc
     1921 gggtggtgcg tcccgccgga cgctaggacc gccgcaaccc ttgcgcggcc gcggcctcgc
     1981 cccggcggtt tgaagacaaa ggcctgacat cgacttcggg tgtcaggctt tctctttcct
     2041 aagctctgac gccgacgtgt gaaacccgct gccgagcact acgtccatct aggtatccgt
     2101 accatggagg acggtatgaa gcgtcagcgg atggcccagg tcgctttgat cctggtgggc
     2161 ttgctctatc tctcctggtt catccccctg ctccaagcac tgtggcagtc cagctggatt
     2221 caagggaatc aggaagcttt tccgatgttc acgacggtca acacggtgct tggggtcttt
     2281 ctcctgctgg cggtcaggag accggcccag catcgcttgc tgatcgcctt tggcgcgtgg
     2341 tcgagcctgg cccatgcgtc cacgatggcg atcatgacgg cggaagcccg ggcgcacggg
     2401 attcccagga gtcctttgga tgtcttcgtc attggcgcgg cgggagtcgt acttctgacg
     2461 gtcctcgcag cgcttcggga aaaacaccca tcgccatccg gtcccgcccc gatcagtgcg
     2521 ccgcgctttg ccgaaggctg atatgcaccc tcgccgtcct accggtggtc ggtgcgcgag
     2581 aagtcgatcc cggccccccc tgcccggccg cggcggctgg cgtggagaat ggtgtgcgcg
     2641 cgggtatcga tctcccaggt ccagatcccc tcggccgtcg ccgatcgcgc cgtcatccgc
     2701 cccggcgcga cgtcctcgac gctcaacgtg tcgtcgacga gcccgccggc ctcatactgg
     2761 tactcacgta tgaccccccg tatgccgcgt tcggccggca gagaaagaat cgccagaggc
     2821 gtatcggtcg aattgaagta ggggggaggc gtcggcgtca ccacgtgatg ggccccaccg
     2881 gagtcggtga cgtcggcaat cgtgctgtcc cggtcaaacc gcaggttcat gatcttcgtg
     2941 gactgatgtg atcgctccga gaccggcacc attccgtcca ccatcagcgt cacgctgtct
     3001 acggtcaggg tcttgccatt caggccggaa aagcgcgaca catactccac cgccttcgcc
     3061 ccgcggatct ccaccgcacg gtactcgaga tgccaggtgg cgacgcgagc cccgacgccg
     3121 gagtccgtca cccgggagac ggcgaactcc atggtctggg gaagggccgc cgacgggatt
     3181 cgaagcggca gcaggggccg ggaggcctgc gcgagtatca agaagagagc aaacgtcaca
     3241 agagtcctcg gatgaaggga atctggccaa cgccctctcg tacggggaag gtgccggtgc
     3301 tgtcacacaa gaacccgtaa gaagaatgtc cggagcctcc ctcccgattc cggacccgcc
     3361 gggttgcaac cttccgcgca tcccccccgt ccacaagccg acaccccttg gagagcctgt
     3421 gcggagaccc tcagtcatcc tctcgctgtc gctgctgatc ctggccaccg ttcgctcggc
     3481 cgcccagatc agcgatccct tacccgccac cggaatcagc ttcgcgggag tggacgagtt
     3541 ctggagcatc ggtggaattc tcagtcacga ctccgagccc accgccgcgc aatggcaggc
     3601 gttgttctct actcccggat accggctggc gttgaagaac ctgggacccg gattgaagga
     3661 cgacatcgat ctcgccctgc ggccatcgcg gcacgccgag tttgtccggg tgagctccgg
     3721 cacggacgat cacagcctga tggtggagca cttggcccaa gcctggtccc gccgggccgt
     3781 gttgcgcgcg tatcgggatt ccctcgcgcg agggacgccc gtcgccgacg cccgcgccct
     3841 ggcctcccgc ttcctccctc cgggcgccac ccggaatggc ccgccgccgc tcgtgacctt
     3901 cgcattcttc aaggacgacg gatattcgtt cccggagggg atcgtcgtcg atctcctcta
     3961 cgcccggtcc gaagcggccg gcggaactcc cctcgtgcga aacctggccc acgaatttca
     4021 ccattcctac gtcaatcgca tggcgcgtcc cgcctccccc ggcgcgacgc cggccgaggg
     4081 tgcccttcgg gacgcgctct acgccttgcg caacgagggt ctcgccgatc tgatcgacaa
     4141 gccctacccg tttcgcgcgc cggtcccagg attggccgcc tatgccgtgc ggtacgacac
     4201 cgagtacgcg cggaccccgg cgaccctccg gacgctcgac tccctcctcg cggcaatcgc
     4261 cggcgattca ggcgccagcg ccgacgccgc gatgcaagcc tcgatgttgt tctggtcgaa
     4321 cgggcatccg aacggcgcgt acgtggcccg cgaaatcctc gaaaccttcg gcgtcgactc
     4381 gctctacccg gcggcccgga gcccggccgc gttccttcgg accttccggt gggcggagcg
     4441 gaagcatggt cgccccgacc cattctcgga cgccgcgcgg cgggaattgg attcgttgga
     4501 cgtccggtac tggcggtgac gcgcaaccag gcccaccacc cacccctccc agatcaccaa
     4561 cgggcgggcc ggccactcga attgccgcac ggctccttga gcaaggtgcc gcaacggcta
     4621 tgctgggtca gcgtttgggt tgccggtgtc cgtcacgctg aaacaggggg ccgaatatgg
     4681 atagaaggat tgtggtcgca gttattgcgt cgctcgtgcc agctgccgtt ggcgctcaag
     4741 cagcacccgc ctcacccacg ccatcggcgc cctaccccac caccatggct cccgtcgacc
     4801 agtaccggat ggatcggagc gcggagatcg ccctggcgaa gagcgcggcc ccggcatcca
     4861 tctcggatag cgccgacgtc atggtcctcg gtgcttcggg atacgagacg gccgtgaaag
     4921 ggaagaacgg gttcgtctgc atcgtgggac gagcgtggtc gagagacttc ggactggacg
     4981 atttctggag tccgaacacg cgggcccccg tctgcttcaa cgcggcagcc gtgcgctcgg
     5041 tcctgccctg gtatctcatc cggacgaagt gggccctctc cgggatgacc gaggcgcaga
     5101 tggaagcccg ggccaggaag tcgccggtgg ccgcgggcgt ccgcgcaccg gagcccggcg
     5161 cggtcggttt catgcagtcg aaggaaggct atatcaacga ggacgtcaaa gggccgtggc
     5221 acccgcacgt gatgttctac gcgcccctca ccaagcccct gaccgacccc accgcgtggg
     5281 gcgcgaacct gcacggctcg cctctccacg tcaacgatgc ggagaccgcg tattacacga
     5341 ccttcgccgt tccggtcacc cagtggtcgg atggtacacc ggataaatag aagcgaacgc
     5401 gaggagtcga ctgaaggaga aagccctgca tcgacgtcga tgcagggctt tttgtcttct
     5461 cagcccggcc ggacccgccc ggtgaaattc aatgggtccc ggagggcctg cagggcccga
     5521 tagtaaaaca gcgccgtcgg atgacggcca tcggtcggga gccgcttcag ccacgtcaat
     5581 tccccctcgg tcgccgttcc gctcatcgtc ggatcattca agaatgcccg gaacccaggc
     5641 tcctcctcag ccggcccctc gaggggttgt tcggtgaact cgaatcgctg atccggcacc
     5701 gtggtcaacc ggcggttcaa aatcaccttg agcccgaagg tcacgagatc gatgtcccac
     5761 gactcgatca gcggccccag aaacgcctcg ccatcccgga tggagaggtg gaagatgtcg
     5821 gtgtcgagga actcgagggt gcgcacccgc gtacgctcgt aactccgccc cgcacgacgg
     5881 gcaaccccgc gggtccattt ctcgttctcc agctcctctt tcgcggcgcg cttcgtgacg
     5941 tccagcagct tttgagccac caagcgctcg agctccccga atggctgctt ttcaaagatg
     6001 gcccggactg cagccgcctt ggcggggtcg cacttgttga gaatgagcgc gcgcaggtcc
     6061 ggaaagagcg ggaccgggac cgcttcgtcg ccgagggtcc gtttcaactg ttcccgccgc
     6121 tggaaggccg ccagacgcgc caactctccc cgggggagct gcaggaactt gtccatccgg
     6181 ccgtagatgt ccgtccggcc cggggccggc ggcgccttct tccgcttcaa gagctgggag
     6241 atgtaagact ccgtgacctg cgccgcccgg gcgagatccc gttgctccag gccgagggtg
     6301 gccaggcgcg cacgaatcaa caggggtacg tccacgcgat tcctcactgg tgacagagtc
     6361 cggaactgaa cttcagttgg ttaagtgata atctaatcga tattcattac ttgacaatag
     6421 tggttcatcc ggatagtatg atgttgattg gcagtgctgg cagcacgctg gagcgcaaaa
     6481 cctgaggagt tgccaaatgt tcgcacggaa cgtcacgctg cacctgaagc cgaacaccgc
     6541 ccccgaattc acccgtaccg tggaaaccga cgtcctgccg gtcatgcgca agcagagcgg
     6601 cttcaaggac gagatcactt tcatcgagcg tcagggcacg gaagccgtcg ccatcagcct
     6661 ctgggagaac aaggagaacg ccgaggccta cggccgc
//
LOCUS       DASUBP010000154         7372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_535846, whole genome shotgun sequence.
ACCESSION   DASUBP010000154 DASUBP010000000
VERSION     DASUBP010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7372
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_535846"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(308..1120)
                     /gene="cdaA"
                     /locus_tag="VFU75_05705"
     CDS             complement(308..1120)
                     /gene="cdaA"
                     /locus_tag="VFU75_05705"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HEU5466364.1"
                     /translation="MNLDAYKFLLPKLWPDAFEILVVAFVIYRFLLFLVGTRAMQIVV
                     GLVIVSLVYAGATVAQLTMISEILRVVFAVAPFAAVVVFQPELRNALAKLGQSRFMRA
                     FARGDRQSVAEEIAEAMGRLSRAGTGAIIAVEGDIGLEEFISSGVPMEAKVSADLLTT
                     IFTPYSPLHDGAVLIRGDQIIGAGCVLPLTQFKVADKSLGTRHRAALGLSEESDATVL
                     VVSEETSTISVASHGILHRPLTPDQVRDLLSGASTNLEGTERLSTRMTAIPE"
     gene            complement(1117..2283)
                     /gene="folP"
                     /locus_tag="VFU75_05710"
     CDS             complement(1117..2283)
                     /gene="folP"
                     /locus_tag="VFU75_05710"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEU5466365.1"
                     /translation="MKLTALAAHTPQAVRAALAARGWESQPAWFASVGIQPLTILIED
                     IGEPEREALVHWGARAGADVLTGEGWALVSTAASRLAVLARPDRAPTELAGLTPEVAG
                     FMVTHAEPRTSWAVAGGSISLVHPVVMGIVNVTPDSFSDGGHYAATDLAIARAEQLAG
                     DGATILDVGGESTRPGAGVVAAEDEISRVVPVIKAIVQRKLGTVSVDTRKAEVARAAL
                     DAGAAIVNDVSGLSFDRRLAAVTAEAGAGIVLMHMRGTPDTMDALANYGHVAAEVAGQ
                     LKEAVGRALDAGIDHARIAVDPGFGFAKDAEQNLRLLDELATVAGVGYPVVVGPSRKR
                     FLGAVTGRPVEDRDRATGVACALAWERGARIFRVHDAALTVEALKLAAAVRPNA"
     gene            complement(2280..4262)
                     /gene="ftsH"
                     /locus_tag="VFU75_05715"
     CDS             complement(2280..4262)
                     /gene="ftsH"
                     /locus_tag="VFU75_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEU5466366.1"
                     /translation="MATRLPPREREFSWSRTFRTFSFWALLLVGAVALVQFAANKKQD
                     TADISFSDFATQLDAQNIAAVEITDKQEVKGDFRHVISYKGHDVEHFTSLLPFDATDA
                     WITNLRTKNVDVTAKEPKQSFGVILLNLWPLLLFAAVMIYLIRQMQQGGNRAFAFGKS
                     KAKLLAGDTPKVTFADVAGCDEAKQELEEIIDFLKDPQRFQRLGGRLPKGALLVGPPG
                     TGKTLLAKAVAGEAGRPFFSMSGSDFVEMFVGVGASRVRDLFEQGKAHAPCIIFIDEI
                     DAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFESNDGVILLAATNRPDILDPALLRPG
                     RFDRQIVVDMPDVKGREQILRVHTRKIPLQPNVDLDRIARGTPGLSGAELSNVVNEAA
                     LLAARRNKQQVENVDLEDAKDKVMLGLERRSMVVTEEERKLTAYHEAGHALVSLHVPS
                     TDPLHKVTIIPRGRALGVTHWLPEEDRHNYTKEYIHSRLATAYGGRVAEELVFGAEKV
                     TTGAAQDIQQATDLARRMVTHFGMSDAVGPIAVGDRESEIFLGREVMQRHEVSNKTAE
                     LVDQELKRILSDAFEKARTILTEHRDQLDRLAMALVERETLDRNEVDLVVAGQPLPPI
                     IRPPTPGSPSPVTPSRPAAIPARGVLGSPPPEPAGA"
     gene            complement(4263..4817)
                     /gene="hpt"
                     /locus_tag="VFU75_05720"
     CDS             complement(4263..4817)
                     /gene="hpt"
                     /locus_tag="VFU75_05720"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEU5466367.1"
                     /translation="MLPSTTRPKQTGGRELARIVFDQETIAKRVAELGQEITAAYPDG
                     ELLVLGLLKGSFIFLGDLVRQIDRPLQVDFLVAASYGADTVSSGTVRLLYDPETRLDG
                     KHILVVEDIVDTGKTLNRLLSILAARSPKSLEICALLHKHVAEQLGREVKFVGFDCPK
                     EFIVGYGLDHAENFRHLPYIASLR"
     gene            complement(4792..6138)
                     /gene="tilS"
                     /locus_tag="VFU75_05725"
     CDS             complement(4792..6138)
                     /gene="tilS"
                     /locus_tag="VFU75_05725"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEU5466368.1"
                     /translation="MGSLSERFLAHVTSHRLFPKPGKALVAVSGGPDSLALLALLHDA
                     REQLGLDLVVAHVDHGIQEASGQVAKSLSRYVKDLGIPFEVAELHLGPDATETEAREA
                     RYAWLRDTRKRLNARYIVTAHHQDDQVETILLRVLRGSAPTGLAGIAAKSRGGLVRPL
                     LPFTKSELSSLTPDPWALTPYEDPANRDQKHLRSWVRTTLLPLITDRLGDRARCDLLS
                     LGRHASRDARAWSALPELLPELDVRFTPGSFSVARLPLAGYDAPVAAALLRAAARRAG
                     IPLGPRHALRLHELAKRQSGRRVELGGGWVGEAVFDRLTVGTVPDHPRETITMREPGS
                     AVFGDYRLVWRMEAAPARMERAGWTTWVSGLESGLVIRAPQAGDRLAPLGGVGRREVR
                     RLLMEARVPRAARAGYPLLAVGETIVWVPGICRGEARVPQPGTPAVRLDVTEYDEA"
     gene            6149..6742
                     /locus_tag="VFU75_05730"
     CDS             6149..6742
                     /locus_tag="VFU75_05730"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEU5466369.1"
                     /translation="MLPPDQVYQLLRNLTSPVVAITSARGSEKNGMIIDSAIRASIVP
                     TIPRIAIFIHKFNYSHQLIHESGKFGLHLLHTGQFDLIHHLGFQSRRAADKLATVPHR
                     TGRLGVPVLEECHAHFECRVVNAMDTGSSTCFLGNVEATGHGGTSMPRGDVMTAAYFR
                     ANLPKAWKGDYERLLKEAQGHAEEASRNIKLEAWIPK"
     gene            complement(6763..7149)
                     /locus_tag="VFU75_05735"
     CDS             complement(6763..7149)
                     /locus_tag="VFU75_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466370.1"
                     /translation="MKRYLALCLLFAMGCSATEPDQNLTGSWTGTFVTTSPAGPTTAW
                     SASLTQTGNAVSGSLDCASTETYAVTGTNVHNATRLTLVGALGDTASLSGTASNHQGV
                     QVSGTFFDNAGAACFSGVGVWQGSIQ"
BASE COUNT         1248 a         2533 c         2395 g         1196 t
ORIGIN      
        1 gctccatctt ctccagcgct tcctgtaccc gattctcacc tacgccggtc aatcccgcat
       61 caagcgccgc ctttacggcg tcgggcccga accccttcgt gacggcgagc accgtcaccg
      121 ggtgcgtcca gccccccgcc acttggcgtc gagcaatctc agcccgtatc tggctcaggc
      181 ggtctgagag cccggcgaaa ctcatagccg cgcaaaatac cgacttggcg ggggggaaac
      241 aacttagcgc gattggggcc gatgttgcta accccgggcg caagcccggg gtggtgcgga
      301 gctgcggcta ctcaggaatt gcggtcatcc gcgtggacag ccgctccgtg ccttccaggt
      361 tggtggaagc cccggagagg aggtcccgga cctggtccgg agtcaggggt cggtggagga
      421 tgccgtggct ggcgacggaa atggtggacg tctcctcgga caccaccagc accgtcgcgt
      481 cgctctcctc ggagaggccg agcgcggcgc ggtgacgggt gccgagggac ttgtcggcga
      541 ccttgaactg cgtaaggggc agaacacatc ccgcgccgat gatctgatcg ccgcgaatga
      601 gcaccgcgcc gtcgtgcagg ggcgaatacg gcgtgaagat cgtcgtgagc aggtccgccg
      661 agaccttcgc ctccatcggt acgcccgacg aaatgaattc ctcgagcccg atgtcccctt
      721 ccaccgcgat gatggcgccc gtcccggcgc gcgacagccg ccccatcgct tcggcgatct
      781 cttccgcgac cgactggcgg tccccccggg cgaaggcgcg catgaaccgc gattgcccga
      841 gtttcgccag ggcgttgcgg agctccggct ggaagacgac caccgcggcg aagggcgcca
      901 cggcgaacac gacccggagg atttcactga tcatcgtcag ctgcgcgacg gtcgcgcccg
      961 cgtagacgag cgagacgatc acgagtccca ccacgatctg catcgcacgg gtgcccacga
     1021 ggaacagcag gaagcgatag atgacgaacg cgacgacgag gatttcgaac gcgtcgggcc
     1081 acagcttggg cagcagaaac ttgtaggcgt cgagattcac gcgttgggcc tcacggcggc
     1141 ggcgagtttc agggcttcca cggtcagagc cgcatcgtgc acccgaaaaa tccgggctcc
     1201 acgttcccag gccagcgcac aggcgacccc cgtggcgcgg tcccggtcct ccaccggccg
     1261 cccggtcacg gctccgagaa accgcttgcg ggacgggccg acgactacag gatagccgac
     1321 gccggcgacc gtggccagtt catcgaggag ccggagattc tgctcggcgt ccttggcgaa
     1381 cccgaacccg gggtcgaccg cgatccgggc gtggtcgatt cccgcgtcca gggcccggcc
     1441 caccgcttcc ttgagttgcc cagccacctc cgcggccacg tgcccgtagt tcgcgagggc
     1501 atccatggta tcgggggttc cccgcatgtg catcagcacg atcccggccc ccgcttccgc
     1561 cgtcacggcc gccagacgcc ggtcgaacga cagacccgac acgtcgttca cgatcgccgc
     1621 ccccgcgtcc agcgccgccc gggccacttc ggccttccgg gtatccaccg agaccgtccc
     1681 cagcttccgc tggacgatcg ccttgatgac cggcacgacc cgggagatct catcctcggc
     1741 tgccacgaca cccgcccccg ggcgcgtcga ctccccgccc acgtcgagga tcgtggcccc
     1801 atcaccggcc aactgctcgg cccgcgcgat cgcgaggtcc gtcgccgcgt agtgaccgcc
     1861 gtcggagaag gaatcgggcg tgacgttgac gatgcccatg acgaccggat gaacgagcga
     1921 aatcgagccg ccggcgacag cccaggacgt cctcggctcg gcgtgcgtta ccatgaatcc
     1981 cgcgacctcg ggggtcaggc ccgccagctc ggtgggggcg cggtcgggac gcgcgaggac
     2041 ggccagacgg ctcgcggccg tcgagaccag agcccacccc tccccggtca ggacgtcggc
     2101 gccggcgcgc gcaccccagt gcaccagggc ttcacgctcc ggttcgccga tgtcttcgat
     2161 gaggatggtg aggggctgga tgccgacgga ggcgaaccag gcgggctggc tttcccagcc
     2221 acgtgccgcg agcgccgcgc gcacggcctg aggcgtgtgc gcggcgagcg cggtgagctt
     2281 cacgctcccg cgggctcggg aggcggagag ccgaggacgc cgcgcgccgg aatggccgcg
     2341 ggacgggaag gcgtgacggg ggacgggctc ccgggggtcg gcggccggat gatcggcggc
     2401 aacggctgcc cggcgaccac gaggtcgacc tcgttgcggt cgagcgtttc ccgctccacc
     2461 aacgccatgg ccaggcggtc cagctgatcc cgatgttccg tcagaatcgt ccgggccttt
     2521 tcgaacgcgt ccgacagaat ccgcttcagc tcctgatcga cgagctcggc cgtcttgttg
     2581 gagacctcgt ggcgctgcat gacttcgcgg cccaggaaga tttccgactc gcggtcaccg
     2641 acggcgatgg gcccgaccgc gtcactcatg ccgaaatgcg tcaccatccg gcgcgccaga
     2701 tcggtggcct gctgaatgtc ctgcgcggcg ccggtcgtca ccttctccgc cccgaacacc
     2761 agctcctccg ccacccgccc gccgtacgcg gtcgcgagac gggagtggat gtattccttg
     2821 gtgtagttgt gccggtcctc ttcgggcagc cagtgcgtca cgccgagcgc gcggccgcgc
     2881 ggaatgatgg tgaccttgtg gagcggatcg gtggacggca cgtggaggct gaccagcgcg
     2941 tggccggctt catggtacgc cgtaagcttc cgctcctctt cggtcaccac catggacctc
     3001 cgctcgaggc caagcatcac tttgtccttg gcatcctcga ggtccacgtt ctcgacctgc
     3061 tgcttgttcc ggcgcgcggc caggagcgcc gcctcgttca ccacgttgga cagctcggca
     3121 cccgagagtc cgggagtgcc gcgcgcgatc cggtccaggt ccacgttggg ctggagcggg
     3181 atcttccggg tgtgaacgcg gaggatctgt tcgcggccct tcacgtccgg catgtcgacc
     3241 acgatctggc ggtcgaagcg gccgggacgg agcagcgcgg gatcgaggat gtcgggccgg
     3301 ttggtggcgg cgaggaggat cacgccgtcg ttcgactcga agccgtccat ctcgaccagg
     3361 agctggttca gcgtctgctc ccgttcgtcg tgcccgccgc cgagtccggc accacgatgg
     3421 cggccgacgg cgtcgatctc atcgatgaag atgatgcacg gcgcgtgggc cttgccctgc
     3481 tcgaagaggt cgcggacgcg ggacgcaccc acgccgacga acatctcgac gaagtcggaa
     3541 ccggacatcg agaagaacgg acggcccgct tctccggcga cggccttggc gagcagggtt
     3601 ttccccgttc cgggcggacc gacgagcagc gcgcccttgg gcaggcgtcc gccgagccgc
     3661 tgaaagcgct gcggatcctt caggaagtcg atgatctcct cgagctcctg cttggcctcg
     3721 tcgcaccccg cgacgtcggc gaacgtcacc ttcggggtgt caccggccag cagtttggcc
     3781 ttggactttc cgaaggcgaa cgcgcggttc ccgccctgct gcatctgccg gatgagatag
     3841 atcatcaccg cggcgaacag gaggaggggc cacagattga ggaggatgac gccgaaggac
     3901 tgcttgggct ccttggcggt cacgtcgacg ttcttggtgc gcagattcgt gatccacgca
     3961 tcggtggcgt cgaagggaag cagcgacgtg aagtgctcca cgtcgtggcc cttgtagctg
     4021 atgacgtgcc ggaagtcgcc cttcacttcc tgcttgtcgg tgatctccac ggccgcgatg
     4081 ttctgcgcat cgagctgcgt cgcgaaatcc gagaagctga tgtccgccgt gtcctgcttc
     4141 ttgttggccg caaactggac caacgccacc gctccgacga gcagcagcgc ccagaacgaa
     4201 aaggtccgga aggtccggct ccagctgaat tcccgctcgc gcgggggcaa acgcgtggcc
     4261 atctaccgca agctcgctat ataaggaagg tgccggaaat tctccgcatg atccaagcca
     4321 taccccacaa tgaactcctt cgggcagtcg aaccccacga acttcacctc acgacccagt
     4381 tgctcggcca catgcttgtg caagagcgcg cagatctcca ggctcttcgg gctccgggcc
     4441 gccagaatcg acaggagccg gttcagggtc ttcccggtgt ccacgatgtc ttccactacc
     4501 aatatgtgct tcccatccag ccgggtttca gggtcgtaca ggagccgcac ggtgccgctc
     4561 gaaacggtgt cggccccgta gctggccgcg accaggaaat cgacctgcag cggccggtcg
     4621 atctgccgca ccagatcccc aagaaagatg aagctcccct tcaacaggcc cagcaccagc
     4681 agctccccgt ccgggtacgc cgccgtgatc tcctggccca attcggccac ccgcttcgcg
     4741 atcgtctcct gatcgaagac gatccgcgcg agctcccggc cgccggtctg cttaggcctc
     4801 gtcgtactcg gtaacatcga gccgcaccgc cggagttcca ggttgcggga cccgggcctc
     4861 accgcgacag atgcccggga cccagacgat ggtttcaccc accgccagga gcggataccc
     4921 tgcccgcgcg gcacgcggca cccgtgcttc catcaacagc cgtcgcactt cccgtcttcc
     4981 cacgccaccc aggggtgcca agcggtcccc cgcctgtgga gctcgaatca cgagaccgct
     5041 ctcgagccct gacacccagg tggtccaccc ggcgcgctcc atccgggccg gtgctgcttc
     5101 catccgccac acgagccggt agtcgccgaa gaccgccgac ccgggctccc gcatggtgat
     5161 ggtttcgcgc gggtgatcgg gcacggtgcc taccgtcagc cgatcgaaga ccgcttcccc
     5221 cacccagcca ccccccaatt ccacgcgccg tcccgactgt cgtttggcca actcatggag
     5281 gcggagggca tgccggggcc cgagcggaat tccggcccgg cgggctgcgg cgcggaggag
     5341 ggccgcggct actggtgcat catacccagc gagggggagc cgggcaacgc tgaaggagcc
     5401 cggcgtgaag cgaacatcaa gctccggaag gagttcgggg agggcgctcc acgccctcgc
     5461 atcccgcgac gcgtgccggc cgaggctcaa gaggtcgcac cgagcccgat cccccagccg
     5521 gtcagtaata agaggaagga gcgtcgttct gacccaggac ctgagatgct tttggtctcg
     5581 gttggcggga tcttcgtaag gggtcagggc ccaggggtca ggggtcaggg atgacaattc
     5641 ggacttggtg aatggcagca ggggccgcac cagtcccccg cgcgatttgg cggcaatccc
     5701 cgccaacccg gtcggcgcac tgccccgcag gacccgcaac agaatcgtct ccacctgatc
     5761 gtcctgatga tgcgccgtga cgatgtacct ggcgttcagc cgtttcctgg tgtcgcgcag
     5821 ccacgcgtag cgcgcttccc gggcctcagt ttccgtcgca tcggggccga ggtgaagctc
     5881 ggccacctcg aacggaatgc ccaggtcctt cacatagcga cttagcgact tagcaacttg
     5941 gccactcgcc tcttgaatcc cgtgatccac gtgggcaacc accaggtcga ggccgagttg
     6001 ctcccttgca tcatggagca aggcgagcag cgcgagcgaa tcgggaccgc cggacacggc
     6061 gacgagcgcc ttccccggct tcggaaaaag ccggtgcgac gtcacgtgtg ccaggaaccg
     6121 ttccgacagg ctgcccatat attcaagtat gctcccacca gaccaggttt accaactcct
     6181 gcggaacctg accagcccgg tcgtcgcgat cacgtcggca cggggatccg agaagaacgg
     6241 catgatcatc gattcggcca tccgcgcttc gatcgtgccc accatccccc gcatcgccat
     6301 cttcatccac aagttcaact acagccacca gctgatccat gaatcgggca agttcggcct
     6361 gcatctcctc cacaccggcc agttcgatct catacaccac ctgggatttc agagccggcg
     6421 cgccgccgac aagctcgcca ccgtgccgca ccgcaccggg cgcctgggcg ttccggtgct
     6481 cgaggagtgc cacgcccatt tcgagtgccg ggtggtgaac gccatggaca ccgggagctc
     6541 gacctgcttt ctcggcaacg tggaagcgac gggacatggg gggacgtcga tgccccgcgg
     6601 ggatgtgatg acggcggcct acttccgggc gaacctgccg aaggcgtgga agggtgacta
     6661 cgagcggctg ctgaaggagg cgcaggggca cgccgaggag gcgtcgcgga atatcaagct
     6721 ggaggcgtgg attccgaagt aactcttcag agtgtgaaat ccctactgaa tgctcccctg
     6781 ccacacgcct acccccgaaa aacatgccgc cccggcattg tcgaagaacg tcccgctcac
     6841 ctgcacgccc tggtggtttg acgcggtgcc ggagagcgag gccgtgtccc cgagcgctcc
     6901 aaccagggtg agcctcgtgg cattatggac gttggtgccc gtcacggcgt aggtctcggt
     6961 cgacgcgcag tcgagtgacc cggacacggc gttcccggtc tgggtcaggg acgcggacca
     7021 ggcggtggtg ggcccggcag ggctggtggt cacgaaggtc ccggtccagc tcccggtgag
     7081 gttctgatcg ggctcggtcg cgctgcatcc catcgcgaaa agcaggcaga gtgcgaggta
     7141 tcgcttcatc tggagaagct ggagtcggtg attcgcaggg tcaaggcgtt tcccgtacct
     7201 tcgaggtgat gcgcgtgctc ctcccggcgt tcctgatcgt tctggcgcaa acgggtgtca
     7261 cccagacggt gacggtgaag cagaacgtca acttgcggcc cgacccctcg agcgagtacg
     7321 cgcccatcag gctgctgaag cccggcgagc cccctctcac cttgctcgat cc
//
LOCUS       DASUBP010000155         2835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1135629, whole genome shotgun sequence.
ACCESSION   DASUBP010000155 DASUBP010000000
VERSION     DASUBP010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135629"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            378..701
                     /locus_tag="VFU75_05740"
     CDS             378..701
                     /locus_tag="VFU75_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5466371.1"
                     /translation="MATALLPPPSRAAQWFHALSDPTRIKIVEMLSHGERCVCELQTS
                     VDAAQSRLSFHLRVLKDAGLVSDRREGRWSYYTLNAHALDQIADFAQAVKPGEHAGSC
                     RAACC"
     gene            752..1564
                     /locus_tag="VFU75_05745"
     CDS             752..1564
                     /locus_tag="VFU75_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HEU5466372.1"
                     /translation="MTDAELITKTVREKYGAAALKIVQGESAASACCGGGCDPVSDNL
                     YAADQVAQLPESAVLASLGCGNPTALADLHPGEIVVDLGSGGGIDVLLSARRVAPGGK
                     AYGVDMTDEMLALARENQRKAGVENVEFLKGSIEALPLPGNHADVVISNCVINLSADK
                     AKVLAEAFRVLKPGGRFAVSDVVIRGEVPPAVRSSMELWVGCVAGALEESEFRDLLTR
                     TGFVDVDIEPTRVYAFEDARAILTGAGLDADQVAREVSGRIMGAFVRGRKPA"
     gene            1561..2706
                     /gene="arsB"
                     /locus_tag="VFU75_05750"
     CDS             1561..2706
                     /gene="arsB"
                     /locus_tag="VFU75_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HEU5466373.1"
                     /translation="MSGARPAVAAPSAPGAPVGSVVRRLSTLDRFLPVWIFLAMAAGI
                     LLGRAFPNLGALLDRAQVEGVSVPIAIGLLWMMYPVLAKVRFESIGRHVTNPRLMGTS
                     LFFNWVLGPIVMFALAWALLPDLPAYRTGLILIGLARCIAMVLIWNSLASGSNELAAV
                     LVALNSVFQILMYSVLGWLFLDVVPGWFGADTALVHVSMWAIAKSVLIFLGVPLIAGY
                     ITQRALIARRGETWYTSVFMPQFGPTALVGLLYTIVLMFAMQGSRIVQLPLDVLRIAA
                     PLLLYFFVMFSLAFTVSKRLGFSYEETASLSFTAAGNNFELAIAVAVATFGIGSGEAL
                     AGVVGPLIEVPALISLVYVSLWAGRRFFGVSSDSLRQGSAHDHPTAS"
     gene            2684..>2835
                     /locus_tag="VFU75_05755"
     CDS             2684..>2835
                     /locus_tag="VFU75_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466374.1"
                     /translation="MTTPPPREYRLLFLCTGNSARSQMAEAILNHRGIGRFRAESAGS
                     KPAARV"
BASE COUNT          421 a          954 c          924 g          536 t
ORIGIN      
        1 cctcgagcgg atcgccgtcc aggatggacg ccatccgccc ctctcacctc ccaagaagac
       61 cactcccgtc taagcccgca gggatacgcc cgccagctgg acccgatcga cgatctggta
      121 gcgtccgggg ccccaagatc aaggatgctc acagtcttcg caggaaccaa aggtccagcc
      181 tctcccttcc ttccacgttc ccggggatcc gccaagagcc ccatgctgcc tcaagcccaa
      241 gcgggtcctt agcgtcgcgc gcggtcgcgc gctcgctcag gatgacggcg cgcttggttt
      301 gtagagaaag gaaaggggag ttgggagggc gtgaagagcc ctgttgacgt atcaatattt
      361 gttgatatat tcctcccatg gccaccgcgc tcctcccgcc cccctcgcgc gccgcgcagt
      421 ggttccacgc gctgtcggac cccacgcgga tcaagatcgt cgagatgctt tcccacggag
      481 agcggtgcgt gtgcgagctg caaacgtccg tcgacgcggc acagtcgcgg ctctcgtttc
      541 atctacgcgt gctcaaggat gcggggctgg tctccgaccg gcgcgaagga cgctggagct
      601 actacaccct caatgcccac gccttggacc agatcgcgga tttcgcgcag gcggtgaagc
      661 ctggtgaaca cgccgggagc tgtcgtgccg cctgctgcta ggggttctct cgtgtctaat
      721 gcatcaacaa aacttgattg atggaggatc gatgaccgac gctgaactga tcaccaagac
      781 cgtccgcgag aagtacggcg ccgcagcgct caagatcgtc cagggcgagt cggcggcctc
      841 ggcctgttgc ggcgggggct gtgaccccgt cagcgacaac ctctacgccg ccgaccaggt
      901 cgcccagctc cccgaaagcg ccgtgctcgc gtccctgggc tgcggcaacc ccaccgccct
      961 ggccgacctc caccccggcg agatcgtggt cgacctgggc tcgggcggcg ggatcgacgt
     1021 cctcctctcg gcccgacggg tggccccggg cggcaaagcg tacggcgtcg acatgaccga
     1081 tgagatgctg gccctggcgc gggagaacca gcgaaaggcc ggggtcgaga acgtcgagtt
     1141 cctgaagggc tccatcgaag ccctgccctt gcccggcaac cacgccgacg tcgtgatctc
     1201 gaactgcgtg atcaacctct cggccgacaa ggccaaggtg ttggcggagg cgttccgggt
     1261 gctgaagccg ggtgggcggt tcgcggtgtc cgacgtcgtg attcgcggcg aggtgccccc
     1321 ggcggtccgg agcagcatgg agctctgggt gggctgtgtc gccggtgcgc tcgaggaatc
     1381 ggagttccgc gatctcctca cccggaccgg attcgtcgac gtcgacatcg agccgacgcg
     1441 ggtctacgcg ttcgaagatg cccgggcgat tctgaccggc gcggggctcg atgcggatca
     1501 ggtcgcccgc gaggtgagcg gccggatcat gggagcgttc gtccgtgggc ggaagccggc
     1561 gtgagcggag cgcgacccgc cgtcgccgcg ccttcagccc ccggcgcccc cgtggggagc
     1621 gtcgtgcgcc gtctctccac cctggatcgc tttctcccgg tctggatttt tctcgccatg
     1681 gcggcgggga tattgctcgg ccgcgccttc cccaacctgg gagcgctctt ggatcgcgcg
     1741 caggtggagg gcgtgtcggt tccgatcgcg atcggcctgc tgtggatgat gtatccggtg
     1801 ctcgccaaag tgcgcttcga gtccatcggc cggcacgtga ccaatccccg gttgatgggc
     1861 acgtcgctgt tcttcaactg ggtgctcggt ccaatcgtga tgttcgccct cgcctgggcg
     1921 ttgctccccg acctccccgc ctaccgcacc gggctcatcc tgatcgggct cgcccggtgc
     1981 atcgcgatgg tgctcatttg gaattcgctc gcctcgggct ccaatgagtt ggccgcggtg
     2041 ctggtcgcgc tcaactcggt gttccagatc ctgatgtaca gcgtgctcgg ctggctcttc
     2101 ctcgacgtcg tgcccgggtg gttcggtgct gacaccgcgc tcgtacacgt gtccatgtgg
     2161 gccatcgcca agagcgtgct gatctttctg ggtgttcccc tgatcgccgg ttacatcacc
     2221 cagcgcgcgc tcatcgcacg ccgcggcgag acctggtaca cgagcgtctt catgccgcag
     2281 ttcgggccca ccgcattggt ggggctgctg tacacgatcg tgctcatgtt cgccatgcag
     2341 ggcagccgga tcgttcagct tccgctggac gtgctgcgga tcgcggcgcc gctcctgctc
     2401 tacttcttcg tgatgttcag cctggcgttc accgtgtcga agcgattggg cttcagctat
     2461 gaggagacag cctcgctctc gttcaccgca gccggaaaca acttcgagct ggccatcgcg
     2521 gtggccgtcg cgacgttcgg catcgggtcc ggcgaagcgc tggctggcgt cgtggggccg
     2581 ctcatcgagg tgcccgccct gatcagtctc gtgtacgtct ctctgtgggc cggccgccgc
     2641 ttcttcggcg taagctccga ctcccttcga caagggtccg cgcatgacca ccccaccgcc
     2701 tcgtgagtac cggctcctct tcctttgcac cggcaattcg gcgcggagcc agatggccga
     2761 ggccattctc aaccaccgcg gaataggccg gttccgggcc gagagcgcgg gatcaaagcc
     2821 ggcggcgcgg gtgaa
//
LOCUS       DASUBP010000156         5319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_154454, whole genome shotgun sequence.
ACCESSION   DASUBP010000156 DASUBP010000000
VERSION     DASUBP010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5319
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154454"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="VFU75_05760"
     CDS             complement(<1..953)
                     /locus_tag="VFU75_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466375.1"
                     /translation="MTLRRLFPFAGIALAAALAACSHGPPPDFAPDPGLVARIRKISI
                     RAPQYACPGQSFAVYYDAYLDDGTRVQFATGYDKNNPPKLHVSFLDFDSFEARPRGDG
                     GWTASPDPLQTVRTGFRLRAILKAKPSIRDSVMLAPDYSCMPHDFHFFGVSGSRGAAG
                     ADGPAVSVRVGIVRSPFYPKLLVSMISVENDPPVFLFADASTIPPANWLTIDSKGGNG
                     SAGRPGAAGSDGVAGQDACPGGAGGNGGNGGAGGSGAPGGRGGQVTVMAPAENPYLAG
                     LVAARAPGGQGGPGGKGGPGGKGGAGGKGMTDARGNKCSDGP"
     gene            1144..2544
                     /locus_tag="VFU75_05765"
     CDS             1144..2544
                     /locus_tag="VFU75_05765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466376.1"
                     /translation="MRKTLAVLTLIALASIARTAAAQDPGSAVPSAMKRGGSPNIRML
                     GHVEVNTGAWKAADIEMEQDADRPYVYVSGFVNNNTKIYDVRDPAHPKLVYTWTIENP
                     ELHRGIGAMDGKYFKIGTHYYYAQSVQYMQGSPDADLGAVILDVTGLPDGSKVHEVAR
                     IHYPASPGGFHNTFAYKHSDGRVLYFATVNQAVALEYDLGKVVSGADSSTWLVGKVPN
                     PTPFRAFGNGGYHDFYVGYDPGTHQDKFYGAGLGGYSVFDVTHPETPKQLFTITGFGM
                     DIAHTFTPSPDGHYAVTETEYQYTPLRVWDLTEGQSGKKANLDLAIAGWTADWHDLSH
                     NHEVRWPFVFVSAYEDGLQVFDLKDPKHPKTDGHYFTCLCKHEDGFGGNPDNGWKTAP
                     DAPSVEQGAFGVDVRNRDGLIAISDMRTGLWLFKMTGFNGWNGHAVGMPNISSVQDYD
                     HGPEGAPAPASGSGGH"
     gene            2541..3014
                     /locus_tag="VFU75_05770"
     CDS             2541..3014
                     /locus_tag="VFU75_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466377.1"
                     /translation="MIKRIGAAFLAAGVLAGGPYDITLLSTPSAPAAHGTARLVSASS
                     PFGIAVSPDGHARYDVVVKIAGLPAPASLGAYHTYVAWAVTPDLNDWTRLGVVSNGSS
                     TVGVAEYNKFLLVITAESSAEATTHGGPTVLHGTSPSGWMQTFLAHPFFRGAPGK"
     gene            3016..4689
                     /locus_tag="VFU75_05775"
     CDS             3016..4689
                     /locus_tag="VFU75_05775"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase family protein"
                     /protein_id="HEU5466378.1"
                     /translation="MRPTLVVVGMLIAGRGVGAQTPDSGMANMPGMGPSMPIPMPRGM
                     VMLPGLVGLAPAVSPFLPGAGRSPSAFPEAGPMMPVELKAGDTLDLTARLVRRTIRGH
                     AYVAYAFNGQIPGPLLRIPQGATVTVRFHNAIDLPSSTHWHGIRLDNRFDGVPGITQD
                     PVLPGSSFVYQVHAPDAGVYWYHPHVREDIEQAMGLAGGILVESRDSGYYAPVNEERV
                     LVLSDLLINGDTLIPFGREEPDFALMGRVGNVLLVNGAPDYRLSVHQGDVVRFFLINA
                     SSSRTYNLSFGGAPMKVVAGDVSRFEREAWVESVVIAPAERYVVEVRFPHPGSYAIVN
                     AVQTINHFIGEFRAEVDTLGEVTADGVPAVPDLSAAFSTLRDNADVTREIDRYRKAMD
                     RPPDKRLTLTMRTHGLPLVTAQFMTIDTAYYAPVEWSDAMPDMNWLSTGDQVTWEIRD
                     DATGKTNMNIDWHVPVGSLVKVRFFNDDKSFHPMQHPIHLHGQRMLVVARDGVKTRDF
                     VWKDTVLIPVGSTVDVLIDAANPGAWMMHCHIAEHLGSGMMTVLHVDPR"
     gene            4853..>5319
                     /locus_tag="VFU75_05780"
     CDS             4853..>5319
                     /locus_tag="VFU75_05780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466379.1"
                     /translation="MVDRRLFLTTASRFGLGATLFPGALAALAQAQGAITKEMVLQAA
                     AIADVPIPPEDVDAMLQTLNNRAAGYETIYKLGMANGVAPALDFDPVPPGMAIDTAQR
                     PSRPTLRAAGSRPKNIQDLCYATIPELGALIRTRKVSSVDLTQMYIARLKRLDP"
BASE COUNT          898 a         1759 c         1758 g          904 t
ORIGIN      
        1 ggcccgtcgg agcacttgtt gcctcgcgcg tccgtcatcc ccttgccgcc cgctcccccc
       61 ttgccgcccg ggccgccctt gcccccgggg cctccctggc ctcccggcgc gcgcgccgcg
      121 acgagtccgg cgagataggg attctcggca ggggccatca cagtgacctg accgccgcga
      181 ccaccgggtg ctccactgcc acccgcaccc ccattccctc cgttgccgcc ggctccaccg
      241 gggcaggcat cctgacccgc cacgccatcg gaaccagccg ccccgggtcg gcccgcgctg
      301 ccgttgccgc ccttcgagtc gatggtgagc cagttggcgg gcgggatggt gctcgcgtcc
      361 gcgaagagaa agacgggggg gtcgttctcg accgagatca tcgagacgag caacttggga
      421 tagaagggcg agcggacgat tcccacgcgc accgacaccg cgggcccgtc cgcgccggcg
      481 gcgccacggc tccctgaaac tccgaagaag tggaagtcgt ggggcatgca gctgtagtca
      541 ggggccagca tgacggagtc cctgattgac ggcttggcct tgagaatcgc cctcagccgg
      601 aatcccgtcc tgacggtctg gagggggtcg gggctcgcgg tccagccgcc gtctccgcgc
      661 ggccgggcct cgaagctgtc gaagtccaaa aaggacacgt gcagcttcgg cgggttgttc
      721 ttgtcgtagc ccgtggcgaa ctgcacccgc gtgccgtcgt ccagatacgc gtcgtagtag
      781 accgcgaagc tctgtccggg acaggcgtat tggggggccc ggatcgagat cttcctgatg
      841 cgggcgacca gtccggggtc gggcgcgaag tccggcggag ggccatggga gcaggccgcg
      901 agggcggcgg ccagggcgat cccggcaaac gggaacaggc ggcgaagtgt catatgtcaa
      961 tctttgaatc gcccgcttct ggtgcaatag gcaacttgct cgccccggcc ccgcgcggca
     1021 tactggcggt gctaattggc gaagtcggaa cgtggaacgc ggagcgcgga accccgagag
     1081 gggcctcgcg ttggcgggtt ccgcattccc ccttccgcgt tccgcgttct aactggagga
     1141 ctgatgcgca agacgctggc cgtactgact ctgatcgcac tagcatccat cgcccgcact
     1201 gcggcggccc aggatcccgg ctccgcggtt cccagcgcca tgaagcgcgg agggtcgccc
     1261 aatatccgca tgctcggcca cgtcgaggtc aataccggag cctggaaagc ggcggacatc
     1321 gagatggagc aggatgcgga tcgtccctat gtgtatgtct cggggttcgt gaacaacaac
     1381 accaagatct acgatgtgcg ggatccggcg catcccaagc tcgtctacac gtggaccatc
     1441 gagaaccctg agctgcaccg cggcatcggc gcgatggacg ggaagtactt caagatcggt
     1501 actcactact actacgccca atcagtccaa tacatgcagg gcagtcccga cgccgatctg
     1561 ggcgccgtca tcctcgacgt cacggggctt cctgacgggt cgaaagttca cgaagtcgcc
     1621 cggattcact atccggcttc gcccgggggc ttccacaaca cctttgccta caagcattcc
     1681 gacggtcgcg tcctctactt cgctacggtc aatcaggccg tggcgctcga gtacgatctc
     1741 ggcaaggtcg tgagcggggc ggattccagc acgtggctcg tgggcaaggt cccgaacccc
     1801 acccccttcc gcgccttcgg gaatggcgga taccacgact tctacgtcgg gtacgatccg
     1861 ggtacccacc aggacaagtt ctatggcgcg gggttggggg gatactccgt cttcgacgtg
     1921 acgcaccccg agacgcccaa gcagctgttc accatcaccg gtttcgggat ggacatcgcg
     1981 cacaccttca cgccgtcccc cgacgggcat tacgcggtga ccgagaccga gtatcagtac
     2041 acacccttgc gggtctggga tctgacggaa gggcagagcg gcaagaaggc gaacctcgat
     2101 ctcgccatcg ccggctggac cgcggactgg cacgatctgt cacacaacca cgaagtgcgc
     2161 tggccgttcg tgttcgtttc ggcgtacgag gatggcctcc aggtgttcga cctgaaggac
     2221 cccaagcacc ccaagaccga cggccactat ttcacctgcc tgtgcaagca cgaagacgga
     2281 ttcggcggca atccggacaa cgggtggaag acggcccccg acgcgccgag tgtggagcag
     2341 ggggcgttcg gcgtggacgt tcgaaaccgc gatgggctca tcgccatttc ggacatgcgc
     2401 accgggctgt ggctcttcaa gatgaccggc ttcaacggat ggaacggaca cgcggtgggc
     2461 atgcccaaca tctcgagcgt gcaggactac gatcacggcc ctgagggggc accagcgccg
     2521 gcgtcgggct cgggcggcca ttgatcaaac gtatcggcgc cgccttcctg gcggcgggtg
     2581 tgttggcagg tgggccgtac gacatcacgc tgctttcgac gccatcagca ccagccgcgc
     2641 atggcaccgc gcggctggtc agcgcgtcct ccccgttcgg catcgcggtg tcccctgacg
     2701 ggcatgcccg gtacgacgtc gtcgtcaaga ttgcggggct cccggcaccc gcgtcgctgg
     2761 gagcgtatca cacctacgtc gcctgggcgg tcacaccgga tctgaacgac tggacccggc
     2821 tgggcgtcgt gtccaacgga tcgtcgaccg tcggcgtcgc cgaatacaac aagttcctgc
     2881 tggtgatcac cgccgagtcg agtgccgagg ccaccacgca tggcgggccc acggtgctcc
     2941 atgggacgtc gccctcgggc tggatgcaga ccttcctcgc gcaccccttt ttccgcggcg
     3001 caccggggaa atgatatgag acccactctc gtggttgtcg gcatgctgat agccggcaga
     3061 ggcgtgggtg cccagacacc cgattcgggg atggcgaaca tgcccggcat gggaccctcg
     3121 atgccgatcc cgatgcctcg aggaatggtc atgctgcccg ggctcgtcgg gctcgcgccc
     3181 gcggtgtctc ccttcctgcc cggagcgggg cgatcgccct ccgcgtttcc ggaggcgggg
     3241 cccatgatgc ccgtcgaact caaggcgggc gacactctcg acctgacggc caggctcgtc
     3301 cgtcgcacca tccgtgggca tgcgtacgtc gcctatgcgt tcaacgggca gattcccggt
     3361 cccctcctcc gaatcccgca gggagcgacc gtgacggtcc ggtttcataa cgccatcgat
     3421 cttcccagct cgacgcattg gcatgggatc cggctcgaca accggttcga cggggtcccc
     3481 gggatcacgc aggacccggt cctcccgggt tcgagtttcg tctatcaggt gcacgcgccc
     3541 gatgcggggg tgtactggta tcacccgcac gtccgggagg acatcgagca ggccatgggg
     3601 ctggcgggcg ggattctggt ggagtcacgg gactccgggt actacgcgcc ggtgaacgaa
     3661 gagcgggtgt tggtcttgag tgacctgttg atcaacgggg atacgttgat tccgttcggc
     3721 cgcgaggagc ccgatttcgc gctcatgggc cgcgtgggga acgtgctgct ggtcaacggg
     3781 gcgcccgact accggctgtc cgtgcatcag ggggatgtgg tccgcttctt ccttattaat
     3841 gcgtcgagct cgcggaccta caacctgtcc ttcggcggcg cgccgatgaa ggtcgtggcg
     3901 ggggacgtga gccggttcga gcgggaggca tgggtggaga gtgtggtcat cgccccggcc
     3961 gagcgctacg tcgtggaggt tcggtttccc cacccggggt cctacgccat cgtgaacgcg
     4021 gtccagacca tcaatcattt catcggcgag tttcgcgccg aggtcgacac gctgggggag
     4081 gtaacggccg acggcgtgcc ggccgtgccg gatctgtccg cggcgttcag caccctgcgt
     4141 gacaatgccg atgtgacgcg ggagatcgac cggtatcgga aggcgatgga tcgtccgccc
     4201 gacaagcgcc tgaccctgac catgcggacc cacggcctcc cgctcgtgac ggcgcaattc
     4261 atgaccatcg acacggcgta ctacgcgccg gtcgaatgga gtgatgcgat gcccgacatg
     4321 aactggctct ccacggggga ccaagtgacc tgggagatcc gggacgatgc gacggggaag
     4381 accaacatga acatcgactg gcacgtgccg gtcgggtcgc tcgtgaaggt gcgattcttc
     4441 aatgacgaca agtccttcca tcccatgcag caccccattc acctgcacgg gcagcggatg
     4501 ctcgtagtgg cacgggatgg ggtgaagacg cgggacttcg tctggaagga cacggtcctg
     4561 atcccggtgg gttcgaccgt ggatgtcctc atcgacgccg cgaacccggg ggcgtggatg
     4621 atgcactgcc atatcgccga gcatctgggg tcgggcatga tgacggtgct gcacgtggat
     4681 ccacgatagc cgcggcctcg aggttgccgg cgcggcatga agccgcggct cggcgccggc
     4741 cgcatgacag cggcgttcgc gccgccaggc ccgtttcacc gggcagacgc cgagccgcga
     4801 ctattacgtc gcgccggcaa ctccgatgcc cggtccagtg aggtaccaac caatggtcga
     4861 ccgccgcttg tttctcacca ccgcctcccg cttcggtctc ggcgcgacac tgtttcccgg
     4921 tgcgcttgcc gcgctggcgc aagcccaggg agcgatcacg aaagagatgg tcctccaggc
     4981 ggcggcgatc gccgacgtcc cgattccccc cgaggacgtc gacgccatgc tgcagacgct
     5041 gaacaaccgc gccgccgggt acgaaaccat ctacaagctc ggcatggcga acggtgtcgc
     5101 gcccgcgctg gacttcgatc cggtcccgcc ggggatggcg atcgataccg cgcaacgccc
     5161 gtcgcgtccc actctgcggg cggccgggag tcgtccgaag aatattcaag acctctgcta
     5221 cgcgacgatc ccggagctcg gcgcgctcat ccgtaccagg aaggtctcgt cggtcgacct
     5281 cacccagatg tacatcgccc ggctgaagcg tctcgaccc
//
LOCUS       DASUBP010000157         7915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_481411, whole genome shotgun sequence.
ACCESSION   DASUBP010000157 DASUBP010000000
VERSION     DASUBP010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7915
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_481411"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..1361)
                     /locus_tag="VFU75_05785"
     CDS             complement(147..1361)
                     /locus_tag="VFU75_05785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466380.1"
                     /translation="MSAVATINRAEIVQKAREAPAARFRMIGAVLAIAGAVALIGGLL
                     TGHAAQVWHAYHFNFLFWTATAQALVIFAASQKLARAHWAGLIIRFAEAAAAFLVLAL
                     VLYLGLILGREYLFKTLPVDRPGVGFWFGSAFLFTRNWLLLALMAWLSWRFVRRDMAP
                     DIAELATGAPATDDVTSKRLVSRDAAILTLCWAFAYSIIGFDFIMSLAPRWISNLFGA
                     FFFMGSFLTALMALALLLHWMRGAMKLEQLITPKQRHDLGKLAFGFTVFWGYLMWAQF
                     LVIWYGNLPEETYFIFYRLWGPWRAVGTAVFLMVFVIPFIGLLGVKPKKTPSTFVLFA
                     LISVAGVWLERYLEVVPSLTPGQGPSLGLPEFGALAFFAGLFLLAYGWFAGKYPMISP
                     RLAVDTLEREQH"
     gene            complement(1358..1894)
                     /locus_tag="VFU75_05790"
     CDS             complement(1358..1894)
                     /locus_tag="VFU75_05790"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HEU5466381.1"
                     /translation="MRKLLPLLAGALVVAACNPDDAVHRVGWFATMRHQRSFKPYAMP
                     RLPAPGTIPITGHDPDSLSVADAGKLTNPRTRTSESLNQGKWVYQTYCQVCHGEAGRG
                     DGPVSTAGGGPFVGVRSLVTDTLARKPDGYIYGVLVYALPMGRGLMPRYGDKVRGTDR
                     WDVVNYIRSLQEQARGAK"
     gene            complement(1887..2426)
                     /locus_tag="VFU75_05795"
     CDS             complement(1887..2426)
                     /locus_tag="VFU75_05795"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HEU5466382.1"
                     /translation="MMNPLKELFGGAMTPGVLGVFGHLDDTTRAVKDLKAEGREDFTV
                     FSPMPRHELEHAIDRPVSSVRMYTLVGGIAGCSIGAWITLYMSYDWPTLVGGKPIGSI
                     PPYVVIMFEMTVLFGALNTLVGVALNALRAARREGTIPYDPRFSNDKFGVFVPCGPDK
                     AGATEAWLKQHGAEEVRRA"
     gene            complement(2426..3784)
                     /gene="nrfD"
                     /locus_tag="VFU75_05800"
     CDS             complement(2426..3784)
                     /gene="nrfD"
                     /locus_tag="VFU75_05800"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HEU5466383.1"
                     /translation="MAQTTAPGTSGVRVTYDYVTRVINNTLGNPSKGYFALLGVAVGL
                     LTIGIICLLFILRYGLGLAGYSHPVYWAVFITCFVFWVGIAHSGTLISAILFLFRSAW
                     RTAVYRVAEAMTVFAVMTAGLFPLIHIGRQWYFYWLVPYPNERGLWPNFKSPLIWDEF
                     AIGTYFTVSTVFLIMGLIPDIAAVRDLATGWRKKLYALTSLGWRGTNEQWRHYSRGYL
                     YLAGLATPLVLSVHSVVSWDFAMAQVPAWHETIFAPYFVAGAIYSGVAMVISLLVPIR
                     KLFHLEEVVTISHFENLAKLCLLTGMIVGFAYATEYFIAWYSGPTPENKAFWYRAFGP
                     FWWAGWTMNVCNWFLPWILWFKKVRTNLTALFVISIFVNVGMWFERFVIIAIHLAGEP
                     FEPFVKAGYNPTWADWGIMAGSFGWFFMWFLLFVKNFPAVSISEVKEVLPPPVRSGKG
                     AH"
     gene            complement(3784..6747)
                     /locus_tag="VFU75_05805"
     CDS             complement(3784..6747)
                     /locus_tag="VFU75_05805"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HEU5466384.1"
                     /translation="MVDGMNRRRFLKVLGVTGGGAAVVAGCGIGPEPTQHLIPYLVQS
                     EDEVPGVPTFYASTCRECAAGCGILVKTREGRAIKIEGNPASPINAGRLCARGQAALQ
                     GLYNPDRLTDPFAKDATGKLAKITWEDAMTRLSGKVRDAKGKGIAFITGLEASSFGDL
                     VDDWMKQVGGRHVTYEPFAFEALREGNRLAFGTAAIPSYDFASAKYILSFGADFMETW
                     LSPVAFQGGFSSAHGFQAGRDAQMAKFVAVSPRMSLEGLNADEWIAVVPGSEGLLALA
                     MAYVIQKSGGAPGDAGRLSDLLNRHRPALVADATGVDAQTIERLAKEFGGAKGGLAVA
                     GGMAAQYPNGAEIVAAVNILNYVAGNVGKTVKFGAELAHGQAGSYRELTALMADMAAG
                     KVAVLITHGANPAYSVGGFTAAAGKVPFRVAFATMLDETASEADLVLPDLHPLEQWGD
                     SRPRAGIFALQQPAMQPVQSNARGAGDVVASLAGKTGTFKDYVQAKWKAPKGQSWADA
                     VQHGGAFGDAPSAGTRLGGDAPKIFAGAPAAALAPGKDEYALIVYPHYALYDGRGADK
                     PWLQELPHPVSKIMWQSWVEVHPDTAATWAVENGDYLLIKTASGGTARVTAWVTPSIR
                     PGVLAMPTGQGHATYGRYAQGRDVNAFDLLPAQANTYGGRTFTVLAKVDVTRDHRFLA
                     TLEGDPREGGESIIELLTLGKAASLHAGQHAFEESASPAYADSAEAGWAAAQKEKTDL
                     GNYANRTIRWAMAIDLSKCTGCSACVTACYAENNIATVGDELTRRRRFMPWMRIERYF
                     NKGENGAIRAVTTPMLCQQCADAPCEPVCPVFAAYHTPDGLNGQVYNRCVGTRYCANN
                     CPYKVRFFNWFNYAERGGEYEAWPTPLDWQLNPDVTVREKGVMEKCTFCVQRIRSAQH
                     SARIEDRTLKDGDIVPACAQGCPSDAIVFGDLNDPASRVHALSEDPRGYHVLQDLNTK
                     PGITYLARVIAGAQA"
     gene            complement(6767..7327)
                     /locus_tag="VFU75_05810"
     CDS             complement(6767..7327)
                     /locus_tag="VFU75_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEU5466385.1"
                     /translation="MTMHGGSRLALATVLLIGASSALAAQAAPRYQAPVVTDTARLTG
                     LRQPIFFRHDIHAGQSQIPCMYCHYTVKVSTEPGIPSLQTCMGCHQLVGGSTPSHQAE
                     IAKVRKAWSDRQPPEWIRVHTLPGFVHFPHMRHIKALGDTSCTLCHGDIAHTTQVAQV
                     QTLRMGWCIRCHVEKKVTRDCTACHY"
     gene            complement(7460..>7915)
                     /locus_tag="VFU75_05815"
     CDS             complement(7460..>7915)
                     /locus_tag="VFU75_05815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466386.1"
                     /translation="TPSAAYWATNLYPSPRQRPKTDSPIRDLPQRAAGRFKRAREGIR
                     ALKHVTEQVVFLGTTWKWVWMYEVGGRKLGYLHPMRSGVSGTFVLNPAEESELGLGDG
                     IPRAVRQAVKDGTMDSGVRWCWMEFDDLERVDAFVEVIRFKHHLLARPE"
BASE COUNT         1402 a         2672 c         2618 g         1223 t
ORIGIN      
        1 ccacgccggc tcccagcagc ccgaacaaga ttttgttcat gggtcccccc gcgaagagtc
       61 aaacatggaa tccctcttga tacgcctgaa actacagggg gctccggcct gagccagagc
      121 ccccatgtgc cggatcggaa cccccgctag tgctgctccc gctccagcgt gtccaccgcc
      181 agacgcggcg agatcatcgg gtacttgccc gcgaaccacc cgtacgccag caggaacagg
      241 ccggcgaaga acgccagcgc accgaactcc ggcaggccca ggctgggccc ttgcccgggc
      301 gtcagcgacg ggacgacctc gaggtaccgc tcgagccaca cgcccgccac cgagatcagc
      361 gcgaagagca cgaaggtcga aggtgtcttc ttgggcttca cgccgaggag cccgatgaac
      421 gggatcacga agaccatgag gaacaccgcg gtgcccacgg cgcgccatgg cccccagagc
      481 cggtagaaga tgaagtaggt ctcttcgggc aggttgccgt accagatcac gaggaactgg
      541 gcccacatga ggtagcccca gaagaccgtg aagccgaagg ccagcttgcc caggtcgtgg
      601 cgctgcttgg gcgtgatcag ctgctcgagc ttcatcgccc cccgcatcca gtgcaggagc
      661 agggcgagcg ccatcagcgc cgtcaggaac gaccccatga agaagaacgc gccgaagagg
      721 ttcgagatcc accggggcgc gagcgacatg atgaagtcga agccgatgat cgaatacgcg
      781 aacgcccagc acagggtcag gatcgccgca tcccggctca ccaggcgctt ggacgtcacg
      841 tcgtccgtgg cgggcgcgcc cgtcgccagc tccgcgatgt cgggcgccat gtcccggcgc
      901 acgaaccgcc acgagagcca cgccatgagc gcgagcagca gccagttccg ggtgaacagg
      961 aaggcgctgc cgaaccagaa gccgacaccc ggccggtcca cggggagcgt cttgaagagg
     1021 tactcccggc cgaggatcag gccgagatag agcaccaggg cgagcaccag gaaggcggcc
     1081 gccgcctcgg cgaaccggat gatgaggccg gcccagtgcg cgcgggcgag cttctggctc
     1141 gcggcgaaga tgaccagcgc ctgcgcggtc gcggtccaga acaggaaatt gaagtggtac
     1201 gcgtgccaga cctgggcggc gtgcccggtg agaagcccgc cgatcagggc caccgcgccc
     1261 gcgatcgcga gcaccgctcc gatcatccgg aagcgggcgg ccggtgcctc gcgggccttc
     1321 tgaacgatct ccgcgcgatt gatcgtggcc acggcgctca cttggccccc ctggcttgtt
     1381 cctggaggga gcggatgtaa ttcaccacgt cccagcggtc agtcccccgc accttgtctc
     1441 cataccgggg catcagtccc cgccccatcg gcagcgcgta gaccagcacg ccgtagatgt
     1501 agccgtcggg tttccgggcc agcgtgtccg tcacgaggga gcggacgccg acgaacggcc
     1561 cgccacccgc cgtcgagacg ggcccatccc cgcgcccggc ttcgccatgg caaacctggc
     1621 aatacgtctg gtagacccat ttgccctggt tgagcgattc cgaagtccgc gtccgcggat
     1681 tcgtcagctt gccggcatcc gccacggaca gcgagtcggg atcgtgcccc gttatcggga
     1741 tggtcccggg ggccggcagg cgcggcatgg cgtacggctt gaaactgcgc tgatggcgca
     1801 tcgtcgcgaa ccagccgacg cgatgcacgg cgtcgtcggg attgcaggcg gccaccacga
     1861 gcgcgccggc gaggaggggc agcagcttac gcacggcgca cctcctcggc cccgtgctgc
     1921 ttcagccagg cctcggtggc gcccgccttg tccggtccgc acggcacgaa cacaccgaac
     1981 ttgtcgttcg agaagcgcgg gtcgtagggg atcgtccctt cccggcgggc ggcacgcaac
     2041 gcgttcaacg cgactcccac gagcgtgttc agtgctccga agagcaccgt catctcgaac
     2101 atgatcacca cgtacggcgg gatcgatccg atcggcttgc cgccgacgag ggtcggccag
     2161 tcgtaggaca tgtagagggt gatccacgcg ccgatcgagc agccggcgat gccgcccacc
     2221 agcgtgtaca tgcgtaccga gctgacgggg cggtcgatcg cgtgctcgag ctcgtgccgg
     2281 ggcatggggg agaacaccgt gaagtcctcc cggccttcgg ccttgaggtc cttcaccgcg
     2341 cgggtggtgt cgtccaggtg accgaacacc cccagcacgc cgggcgtcat cgcgccgccg
     2401 aagagctcct tcagggggtt catcatcagt gcgccccctt cccgctccgc accggcggcg
     2461 gcagcacttc cttcacttcg gagatcgaaa cggccgggaa gttcttcacg aagagcagga
     2521 accacatgaa gaaccagccg aacgagccgg ccatgatgcc ccagtcggcc caggtcggat
     2581 tgtacccggc cttcacgaag ggctcgaagg gctcaccggc caggtggatc gcgatgatca
     2641 cgaagcgctc gaaccacatg ccgacgttca cgaagatcga gatcacgaag agcgccgtga
     2701 ggttggtccg gaccttcttg aaccacagga tccacggcag gaaccagttg cacacgttca
     2761 tggtccagcc ggcccaccag aagggcccga acgcccggta ccagaacgcc ttgttctccg
     2821 gcgtcggacc ggagtaccac gcgatgaaat actcggtggc gtaggcaaat ccgacgatca
     2881 tcccggtgag caggcagagc ttggccaggt tctcgaagtg gctgatggtc accacttcct
     2941 cgagatggaa cagcttccgg atgggcacca gcagggaaat caccatcgcg acgcccgagt
     3001 agatggcgcc ggccacgaag tacggggcga agatggtctc gtgccacgcc ggaacctgcg
     3061 ccatcgcgaa gtcccaggac acgacgctgt gcacggacag cacgaggggc gtcgccaacc
     3121 cggcgagata gagataaccg cgcgagtagt gccgccactg ttcgttggtg cccctccatc
     3181 ccagggaggt cagcgcatag agcttcttcc gccatccggt ggccaggtca cgcaccgccg
     3241 cgatgtcggg aatcaagccc atgatgagga acaccgtgga caccgtgaaa taggtgccga
     3301 tggcgaactc gtcccagatc aggggcgact tgaagttggg ccagagtccc cgctcgttcg
     3361 gatacggaac cagccagtag aagtaccact ggcggccgat gtgaatgagc gggaagaggc
     3421 cggccgtcat caccgcgaac accgtcatcg cttccgcgac gcggtacacc gccgtgcgcc
     3481 acgcggaccg gaacaggaac aagatggctg aaatcagcgt gccggagtgc gcgataccca
     3541 cccagaacac gaagcacgtg atgaagaccg cccaatacac cgggtgcgaa tagcccgcga
     3601 gtcccagtcc gtaccggagg atgaacagca ggcagatgat cccgatggtg agcagaccca
     3661 ccgcgacccc gagcaacgcg aagtatccct tgctcggatt gcccagcgtg ttgttgatga
     3721 cgcgggtgac gtaatcgtac gtcacgcgaa cgcccgacgt gccgggtgcc gtggtctgtg
     3781 ccatcaggcc tgcgctccgg cgatgacgcg cgccagatag gtgatgccgg gcttcgtatt
     3841 gaggtcctgc agcacgtggt acccccgcgg atcctcgctg agcgcgtgca cccgtgacgc
     3901 gggatcgttc agatcaccga acacgatggc gtccgacgga cagccctgcg cgcacgccgg
     3961 cacgatgtcg ccgtccttga gcgtgcggtc ttcgatgcgc gcggagtgct gcgcggaccg
     4021 gatgcgctgg acgcagaagg tgcacttctc catcacgccc ttctcgcgga ccgtcacgtc
     4081 ggggttcagc tgccagtcga gcggagtcgg ccaggcctcg tactcgcccc cacgctcggc
     4141 gtagttgaac cagttgaaga accggacctt gtaggggcag ttgttcgcgc aatagcgcgt
     4201 gcccacgcac cggttgtaga cctgaccatt caagccgtcc ggcgtgtggt aggccgcgaa
     4261 caccgggcag accggctcgc atggggcatc ggcgcactgc tgacagagca tcggcgtcgt
     4321 gaccgcccgg atggctccgt tctcaccctt gttgaagtac cgctcgatcc gcatccacgg
     4381 catgaagcgc cggcggcggg tcagctcatc gccgacggtg gcgatgttgt tctccgcgta
     4441 acacgcggtg acgcacgccg agcagcccgt gcacttcgac agatcgatcg ccatcgccca
     4501 ccgtatggtc cgattggcgt agttcccgag gtccgtcttc tccttctgcg cggcggccca
     4561 accggcctcc gcggagtcgg cgtaggccgg cgatgcggat tcctcgaacg catgctgccc
     4621 cgcgtgcagg ctcgccgcct tcccgagggt caacagctcg atgatgctct cgccaccctc
     4681 acgcggatcc ccttcgaggg tcgcgaggaa ccggtggtcg cgcgtgacgt ccaccttcgc
     4741 gagcaccgtg aaggtccggc cgccgtaggt gttggcctgc gcggggagca ggtcgaaggc
     4801 gttgacgtcg cggccctgtg cgtagcggcc gtaggtcgca tgtccctgcc ccgtcggcat
     4861 ggcgaggacc ccgggacgga tcgagggcgt gacccacgcg gtcacccgtg ccgtgccgcc
     4921 ggacgcggtc ttgatcagca ggtagtcgcc gttctcgacc gcccaggtcg ccgcggtgtc
     4981 ggggtgcacc tccacccacg actgccacat gatcttcgag accgggtgcg ggagctcctg
     5041 cagccagggc ttgtcggcgc cgcggccgtc gtacaaggcg taatgcgggt agacgatgag
     5101 ggcgtactcg tccttgcccg gcgccagcgc ggcggcgggc gcccccgcga aaatcttcgg
     5161 ggcgtcgccg ccgaggcggg tgccggccga cggcgcgtcg ccgaacgcgc caccatgctg
     5221 cacggcatcg gcccagctct gccccttcgg cgccttccac ttggcctgca cgtagtcctt
     5281 gaacgtgccc gtcttccccg cgagcgaagc gaccacgtca ccggcgcccc gggcgttcga
     5341 ctgaacaggc tgcatcgcgg gctgctggag ggcgaagatg ccggcgcgcg gccgggagtc
     5401 gccccactgc tcgagcggat gcagatcggg caggacgagg tcggcctcgc tggcggtctc
     5461 gtcgagcatg gtcgcgaacg ccacccggaa ggggactttg ccggcggcgg cggtgaaccc
     5521 gccgaccgag taagccgggt tggccccgtg ggtgatcaga accgcgacct tccccgcggc
     5581 catgtcggcc atcaaggccg tgagctcgcg gtacgagccc gcctggccgt gcgccagctc
     5641 ggcgccgaac ttcaccgtct tgccgacgtt gccggcgacg taattgagga tgttgaccgc
     5701 cgcgacgatc tcggcgccat tcggatactg cgccgccatg ccgccggcga ccgccaggcc
     5761 acccttcgcg ccgccgaact ccttggcgag ccgctcgatg gtttgcgcat cgacgccggt
     5821 cgcatccgcg accagcgcgg gccggtgacg attgagcagg tcggagagcc ggcccgcatc
     5881 gcccggagca ccaccggact tctggatcac gtaagccatc gcgagcgcca ggagtccctc
     5941 ggaccccggg acgacggcga tccactcgtc cgcgttcaac ccctcgaggg acatccgggg
     6001 gctcaccgcg acgaacttgg ccatctgcgc atcccggccc gcctggaatc catgagcgct
     6061 ggagaaaccg ccctggaacg ccacgggcga cagccaggtc tccatgaagt cggcgccgaa
     6121 cgagaggatg tacttggccg aggcgaaatc gtacgacgga atggcggcgg tcccgaacgc
     6181 gaggcgattg ccctcgcgga gcgcttcgaa ggcgaacggc tcgtacgtga cgtgccggcc
     6241 gccgacctgc ttcatccagt catccaccag gtcgccgaag ctcgaagctt ccaggccggt
     6301 gatgaacgcg atgcccttgc ccttcgcgtc cctcaccttg cccgagaggc gcgtcatggc
     6361 gtcttcccag gtgatcttcg cgagcttccc ggtcgcgtct ttcgcgaacg gatcggtcag
     6421 ccggtcagga ttgtagagtc cctggagcgc cgcctggccg cgggcgcaga ggcgccccgc
     6481 gttgatcggc gacgccgggt tcccctcgat cttgatggcc cgtccctcgc gcgtcttcac
     6541 caggatgccg cacccggccg cgcattcgcg gcaggtgctg gcgtagaacg tgggaacgcc
     6601 ggggacttcg tcttccgact ggaccaggta agggatcagg tgctgggtcg gctccgggcc
     6661 gatgccgcac ccggccacca ccgccgcgcc gcccccggtc acgccgagga ccttgaggaa
     6721 ccggcgccgg ttcatcccgt cgaccatgtc gtctccgctg atcatgctag tagtgacacg
     6781 ccgtgcagtc ccgcgtcacc ttcttctcca cgtgacagcg gatgcaccag cccatccgga
     6841 gcgtctgcac ctgcgcgacc tgagtcgtgt gcgcgatgtc accgtgacag agcgtgcagg
     6901 aggtgtcccc gagagccttg atgtggcgca tatgcgggaa gtgcacgaac ccgggcaggg
     6961 tgtgcacgcg gatccactcg ggcggctgcc tgtcgctcca ggcctttcgg accttcgcga
     7021 tttcggcctg atgggacggc gtcgaaccgc cgacgagctg atggcacccc atgcaggttt
     7081 gcagcgacgg aatccccggc tcggtggaga ctttcaccgt gtaatggcag tacatgcagg
     7141 ggatttggga ttgaccggcg tggatgtcgt gacggaaaaa aatcggttgg cggaggcccg
     7201 tgagccgggc cgtatccgtc acgacaggag cttggtatct gggcgccgct tgggcggcga
     7261 gcgcggaact ggcgcctatg agcaggacgg tcgcgagcgc gagcctgctc cctccgtgca
     7321 tcgtcatcaa tgcgtgggcc tcgatggcga aatcaagtcg agtcggaaag cctcggcgcc
     7381 tgggcctcgg cgcctgtgaa cggctagcgg gcgccgtgaa tgtagcgcct atcgggaacc
     7441 agacacaagc ggggaaccgt cattccggtc gggccaaaag gtgatgcttg aaacggatca
     7501 cctcgacgaa cgcatccacc cgttcgaggt cgtcgaactc catccaacac cagcgcacgc
     7561 cggagtccat ggtcccatcc ttcaccgcct gccggaccgc gcgtggaatg ccgtctccca
     7621 accccagctc agattcttca gccgggttga ggacgaaggt cccgctgacg ccagaccgca
     7681 tcgggtggag atatcccagc ttccggccgc cgacctcgta catccaaacc catttccagg
     7741 tggtgccgag gaacaccacc tgctcggtga catgcttgag cgcccggatg ccctcgcgtg
     7801 cgcgtttgaa ccggcccgcg gcgcgctggg gcaaatcccg aataggagag tcggttttcg
     7861 ggcgctggcg gggactggga tacaggttcg tcgcccaata ggctgcggag ggggt
//
LOCUS       DASUBP010000158         4566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_140715, whole genome shotgun sequence.
ACCESSION   DASUBP010000158 DASUBP010000000
VERSION     DASUBP010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4566
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140715"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(121..1185)
                     /locus_tag="VFU75_05820"
     CDS             complement(121..1185)
                     /locus_tag="VFU75_05820"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEU5466387.1"
                     /translation="MASHNGLQCLVVDDEPRLRRVLVRLLEGDGFVCREAGSGVEALE
                     ALDSQPVPLVLSDVRMPLMDGVALLREVIKRWPDTAVVMVTAVADVDSAVNCLQLGAL
                     DYVAKPFHLDEVRARVAQALDKRRLLLENKNYHRQLEERVEVQARRLEEMFIEGVEMM
                     ADAQEAKDPYLQGHSLRVAEYALHAGRTLGLDATQLGTIFLGGRLHDVGKIGVREEIL
                     HKPGKLSDAEYRHIMEHPVVGARMLARMLRDEPMALAIVRSHHERFDGKGLPDGLEGD
                     QIPVPVRLVSVADSFDAMTSARPYRPPLPVDHAVDELKRMSGKQFWPDAVRAFLDAFP
                     DASKLPIATPAPAGGNGSGH"
     gene            1242..2411
                     /locus_tag="VFU75_05825"
     CDS             1242..2411
                     /locus_tag="VFU75_05825"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEU5466388.1"
                     /translation="MSRAFDKDLQRRFGTRAVHAGQTLDPLAGAVMSPIYQTSTYIQE
                     ALGRHKGYEYARTQNPTREALERCVAALEGGRHGFAFGSGLAALDAILKLLSAGDHVV
                     CGENVYGGTHRAMTHIWSRMGLKFTFVDTGDADEIRKALTPATRMIYVETPTNPMMRL
                     TDLEAAGEIAKKAGALFAVDNTFASPIFQQPLAFGADVVLHSTTKYLNGHSDMIGGML
                     VTDRDDLGERIGFLQNSSGAVPGPMDCWLALRGIKTYPLRMRQHDANGRRIAEWLSHH
                     PKVKQVYYPGLPSHPQHDLACRQMKGFGAMLSLELGDVERARKFAQGTEIFSLAESLG
                     GVESLIGHPASMTHASVPVEMRKKMGLTDSLVRLSVGIEDPEDLIADLDHALAAS"
     gene            2478..3476
                     /locus_tag="VFU75_05830"
     CDS             2478..3476
                     /locus_tag="VFU75_05830"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HEU5466389.1"
                     /translation="MTDPTNLPAARQRKILTQIAPVTWEHAADRAALQTLRSVPGFDE
                     VVKKIYGFLGERGIRLIFQADAVRVGPTQFPRLNALYTDVLTSLDWPERPELFVSQTP
                     FVNAGAFGMDKPFIVLNSGSLKLLDDDELRVLLGHELGHVMSGHALYHTVLVLILSVS
                     LSALPFLAGMAILPIELALLEWFRKSELSSDRAGLLASQDPNASLRLHLKLAGGGDMK
                     EMDLNAFLVQAKEYEESGGALDRIFKILGVLRRSHPFTTIRAAELQRWIEGGNYDRII
                     RGEYTRRGPEADQRPVESDIDDAREHYMKEAKAVAKDVVDGARRAAQAFSDAFKKK"
     gene            3473..>4566
                     /gene="purD"
                     /locus_tag="VFU75_05835"
     CDS             3473..>4566
                     /gene="purD"
                     /locus_tag="VFU75_05835"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEU5466390.1"
                     /translation="MRVLVVGGGGREHALLWALKRDGTAKTLFAAPGNPGTALLGTNL
                     PIPATNVDGLVTATRDQKIDVVIVGPEAPLAAGLADRLRQDGRAVFGPGARGARIEAS
                     KAYAKDLMVKAGVPTAKSGTFTALKPALRYIEAGREPVVVKASGLAGGKGAIVCATKD
                     DATQAAEALLEGGALGEAGREIVIEEFLEGEELSVLALTDGERIVILPPAQDHKRVRE
                     GDEGPNTGGMGAYTPVGLTTPALLTRVRQEVLAPTLKALANDGAPYSGVLYAGLMVAP
                     DGTPNVIEFNCRFGDPETQALFPVLPKGSTSHLTAIASGKWQPDRDVIEPVAAAVTTV
                     LAAPGYPESPRLGETIRLPNEWPEDQILFHA"
BASE COUNT          787 a         1560 c         1506 g          713 t
ORIGIN      
        1 gtcccgaacg ccgcggcata ccgcgcgacg ttctccgggg tgagatcggt gcccacgatg
       61 ccccggattc ccgaaactcc caccatcagc gtgttcgaca tgggcaggaa gataaggggt
      121 ctagtgcccc gagccgttgc ccccggccgg cgccggcgtg gcgatcggca gtttggaggc
      181 atcggggaag gcgtccagga aagccctgac ggcgtcaggc cagaattgct tcccactcat
      241 gcgcttgagc tcgtccaccg catggtccac cggaagagga ggccggtagg gacgggcgct
      301 ggtcatggca tcgaacgaat ccgcgacgga gaccaggcgc accgggacgg ggatctgatc
      361 cccctcgagt ccgtccggga gacctttgcc gtcgaagcgc tcgtgatggg accgcacaat
      421 ggcgagcgcc atcggttcat cccggagcat ccgtgccagc atcctggctc caaccaccgg
      481 gtgctccatg atgtggcggt actccgcatc cgagagcttg ccgggcttgt gcagaatttc
      541 ttcgcggacg ccgatcttgc cgacgtcgtg caggcggccg ccgaggaaga tcgttcccag
      601 ctgagtcgcg tcgaggccaa gcgtccgtcc cgcgtggagc gcgtactctg cgactcggag
      661 cgagtgaccc tggaggtagg gatccttcgc ttcctgcgca tccgccatca tctcgacgcc
      721 ctcgatgaac atctcctcga gacgccgcgc ctggacttcc acgcgctctt cgagctggcg
      781 gtgatagttc ttgttctcga gcagcaggcg gcgcttgtcg agcgcctgcg cgacgcgggc
      841 gcgaacctcg tccagatgga acggcttggc gacgtagtcg agggcgccca gctggagaca
      901 attcacggca ctgtccacgt cggccacggc ggtcaccatc accacggccg tatccggcca
      961 gcgcttgatc acttcccgca gcagcgcgac gccgtccatg agcggcatgc gcacgtcgga
     1021 cagcacgagg ggcacgggct ggctgtcgag cgcttcgagc gcttcgacac ccgatcccgc
     1081 ttcccggcag acgaacccgt cgccctcgag gagccggacc aggacccgcc gcagacgcgg
     1141 ctcgtcgtcc acgacgagac attgcaaccc gttatgtgag gccatcatcg ctattgtacg
     1201 gctcgcttgc ccggtgggga aggcgccctt acgttccgcc tgtgagccga gccttcgaca
     1261 aggacctgca gcgccgcttc ggcacgcgcg cggtccacgc cggacagacc ttggacccgc
     1321 tcgcgggcgc ggtcatgtcc cccatctatc agacctcgac ctacatccag gaagcactgg
     1381 gccggcacaa gggatacgag tatgcccgga ctcagaatcc tacccgcgag gcgctcgagc
     1441 gctgcgtcgc ggcactcgag gggggacggc acgggttcgc cttcggctcc gggctggcgg
     1501 cgctcgacgc gatcctgaag ctgctgtcgg ccggggacca cgtggtctgt ggcgagaacg
     1561 tgtacggcgg gacccaccgc gcgatgacgc acatctggtc ccgaatgggt ctcaagttca
     1621 ccttcgtcga tacgggagac gccgacgaga tccggaaggc cctgacgccc gcgacgcgaa
     1681 tgatctacgt cgagacgccg acgaacccga tgatgcggct caccgatctc gaagccgccg
     1741 gagaaatcgc caaaaaggcc ggcgccctct tcgcggtcga caataccttc gcctccccca
     1801 tcttccagca gcccctcgcg ttcggcgccg acgtcgtcct ccactccacc acgaagtatc
     1861 tgaacgggca ctccgacatg atcgggggca tgctggtcac cgatcgcgac gacctggggg
     1921 agcgaatcgg attcctgcag aacagctccg gcgccgtccc cggaccgatg gactgctggc
     1981 tggccctgcg cgggatcaag acctatcccc tgcgcatgcg gcagcacgat gccaacggcc
     2041 gccgcatcgc cgagtggctc tcgcaccacc ccaaggtgaa gcaggtgtat taccccgggc
     2101 tcccgtcgca tccgcagcac gacctggcct gccggcagat gaagggcttt ggtgccatgc
     2161 tgtcgctcga gctgggagat gtggaacggg ccaggaagtt cgcccagggg accgagatct
     2221 tctctctggc cgagtcgctg ggcggggtgg aaagcctgat cgggcacccc gcatccatga
     2281 cccacgccag tgtcccggtg gagatgcgca agaagatggg actgaccgac tccctggtcc
     2341 gcctctccgt cgggatcgag gaccctgaag acctgattgc ggacctggac cacgccctgg
     2401 ccgcctcgta ggcgggccga aaccctgcac gggattcggc gtaggttcag gaaccccgaa
     2461 agcttccgga gcgccgtatg accgatccca cgaacctgcc ggccgcgcgt cagcggaaaa
     2521 tcctcaccca gatcgcgccg gtcacatggg agcacgccgc ggaccgcgcg gcgctccaga
     2581 ccctgcggtc ggtgccgggc ttcgatgaag tggtgaagaa gatctacggc tttctggggg
     2641 agcggggcat ccgcctcatc ttccaggccg acgccgtccg ggtggggccg acgcagttcc
     2701 cccggctcaa cgccctgtac accgacgtcc tgacctccct cgactggccc gagcggccgg
     2761 agctgttcgt ctcgcagacg cccttcgtca acgccggcgc cttcggcatg gacaagccct
     2821 tcatcgtcct gaattcggga tcgctcaagc tcctcgatga cgacgagctc cgcgtcctcc
     2881 tcggtcacga gctcggtcac gtgatgagcg ggcacgccct ctatcacacc gtcctcgtgc
     2941 tcattctctc cgtgagcctc tcggccctgc ccttcctggc cgggatggcc atcctgccga
     3001 tcgagctggc gttgctcgag tggttccgga agagcgagct gtcgagtgac cgtgccggcc
     3061 tcctggcctc tcaggatccg aacgcgtccc tccggctcca tttgaagctc gccggcggcg
     3121 gcgacatgaa ggaaatggac ctgaacgcgt ttctcgtgca ggccaaggaa tacgaagagt
     3181 cgggcggggc gctggaccgc atcttcaaga tcctgggcgt gctgcggcgc agccatccgt
     3241 tcacgaccat ccgcgcggcc gaattgcagc ggtggatcga gggcgggaac tacgaccgga
     3301 tcatccgtgg cgagtatacc cgccgcgggc ccgaagccga tcagcgtccc gtcgagagcg
     3361 acatcgacga cgcccgcgag cactacatga aggaagcgaa ggcggtggcc aaggacgtcg
     3421 tggacggcgc ccggcgggcc gctcaggcgt tctcggacgc gttcaagaag aagtgagagt
     3481 cctggtcgtc ggcggagggg gccgtgagca tgccctgctc tgggccctga agcgcgacgg
     3541 cacggcgaaa acgttgttcg cggcccccgg gaatcccggg accgccctcc tcggtaccaa
     3601 cctccccatc cccgccacga acgtcgacgg actggtcacc gccaccaggg accagaagat
     3661 cgacgtcgtg atcgtcggac ccgaagcgcc actcgccgcg gggttggctg accgactgcg
     3721 ccaagacggc cgcgccgtgt tcggtcccgg agcgcggggc gcgcgcatcg aggcctccaa
     3781 agcctacgcg aaggacctga tggtcaaagc cggcgtgccg accgcgaaga gcgggacctt
     3841 cacggcgctg aagccggccc tcaggtacat cgaggcgggg cgcgagccgg tggtggtgaa
     3901 ggcctcaggg ttggcggggg gcaagggcgc gatcgtctgt gccacgaagg acgacgcaac
     3961 ccaggccgcc gaggcgctcc tcgaaggggg agcactgggc gaggcgggca gggaaatcgt
     4021 gatcgaggag tttctcgagg gcgaggagct ctcggtcttg gcgctgaccg acggtgagcg
     4081 catcgtcatc cttccaccgg ctcaggacca caagcgggtg cgcgagggag atgaaggccc
     4141 caacaccggc gggatgggcg cctacacgcc ggtcgggctc acgacccccg ccctgctcac
     4201 gcgggtgcgc caggaggtcc tcgcgccgac gttgaaggcc ctcgccaacg acggggcacc
     4261 gtactcgggc gtgctgtatg ccggactgat ggtggctccc gacgggaccc cgaacgtgat
     4321 cgagttcaac tgccgcttcg gcgatcccga gacgcaggcc ctcttccccg tactgcccaa
     4381 gggctcgacg tcccacctca cggcgattgc gtccggcaaa tggcaaccag atcgcgacgt
     4441 gatcgaaccg gtggcggctg cggtcactac ggtgctcgcg gccccggggt accccgaatc
     4501 gcccaggctc ggcgaaacga tccggctgcc caacgagtgg cctgaagacc agatcctgtt
     4561 tcacgc
//
LOCUS       DASUBP010000159         6264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1162615, whole genome shotgun sequence.
ACCESSION   DASUBP010000159 DASUBP010000000
VERSION     DASUBP010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6264
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1162615"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1375
                     /locus_tag="VFU75_05840"
     CDS             <1..1375
                     /locus_tag="VFU75_05840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEU5466391.1"
                     /translation="ERDPGPVSEAVAPSGPAGKLTTGDLRSTILRLAAPTVAMMAFNT
                     GFNVIDSIWVGQLIGPAALAAVSTAGFTVWVMMSLAEMLDVGVVALAAHRHGARAPDQ
                     AARVAAAALVTGLVAGLLASFAGLAALPKLFDLMQVPPEVAVLGHTYLHTWLLGAPLV
                     FGFFALEATFRSSGDTTTPFMVLAASAVVSVILDPLLIAGIGPFPRLGVEGAALASVM
                     VRGVACLALVWLAFRRGHIHFRAPDWASAPHILRIGLPYSMSGILFSLVYMWLTRFTS
                     RFGTPALAALGVGHKVEGVGFVAITGFTLAAAALVGQSLGAKDEARARKAAWHTVGYA
                     LLVTTCTALACLLVPRFFVRLFTSDPQVIANGTGYLRIIAMAQIGQTFELILEGALAG
                     AGYTFWPMVWSSSLTFARIPLAAWWSGIWGVTGIWWAISLTATLRGVAMTVLWLSGAW
                     RPVPRTA"
     gene            1372..2115
                     /locus_tag="VFU75_05845"
     CDS             1372..2115
                     /locus_tag="VFU75_05845"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HEU5466392.1"
                     /translation="MNSIITLLTDYGAGDSYVAEVKGVILTAVPDATLIDVTHQVSPG
                     DVRAGQFLFNRAWRRFPAGTVHLVVVDPGVGTSRRALAAEAHGHFFVAPDNGLLTSLP
                     EDAEFVSLPVPPSASPTFHGRDVFAPAAARLAMGVSLDELGDPVDDVRRSPLPEPRAD
                     GVALLGEVIYVDRFGTLVSNIRAEDVDEGVRITVEGNDVGALRRTFGDVEPGQLVAYT
                     GSGGEVEIAVRGGSAARLLGVGVRAEVRA"
     gene            complement(2292..2537)
                     /locus_tag="VFU75_05850"
     CDS             complement(2292..2537)
                     /locus_tag="VFU75_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466393.1"
                     /translation="MNERLQTHLTPEEVELWATGLLSAARTFHLTECAECLAVAERER
                     KLLVELAQLQRFAPSADFAEKVLAQVRIPTPSGSHHG"
     gene            complement(2572..3132)
                     /locus_tag="VFU75_05855"
     CDS             complement(2572..3132)
                     /locus_tag="VFU75_05855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5466394.1"
                     /translation="MVRWARDGHEPAFRELVHRYERPVFSLIYRMVRDRALAEDLAQE
                     TFIKVLNALKSYKPEYKFSSWIFKIANNAAIDHLRRRQLDTLSLDGSPTARSPEEMHA
                     SAPQLGDVSESPLDEVESRELGGAIERAIGRLRPEYRSCILLRHVEGHSYEEIAQMLD
                     LPLGTVKTYIHRARNELRVYLEDARE"
     gene            3257..3916
                     /locus_tag="VFU75_05860"
     CDS             3257..3916
                     /locus_tag="VFU75_05860"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HEU5466395.1"
                     /translation="MFTAIAPRYDFLNHLLSLNVDRRWRRRAVARLGWERRPDGLYLD
                     LCAGTLDLAAALASAEGFRGRIIGADFVIPMLQRGRGKAAAVRPVGADALELPFPNEG
                     FDGALVGFGVRNLADLDLGLSEARRVLKPGARLVILEFTTPRFAPLRAAYLFYFQRLL
                     PLIGRTVSKHTDAYTYLPESVLNFPDPDALSRRLETAGFSDVRYELLTGGICAIHHGT
                     R"
     gene            3903..5384
                     /locus_tag="VFU75_05865"
     CDS             3903..5384
                     /locus_tag="VFU75_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis decarboxylase"
                     /protein_id="HEU5466396.1"
                     /translation="MALDNLRDFLAALDEAGELVRVHRPVLVDREITEIADRCMKSPG
                     GGPALLFERPTLTSGGASPHPVAVNLFGSQKRMCMALGVAKLDEIGDRVSEFVNLKVP
                     DGLVGKLAMLPKLAELTKFPPRSGGGSPACQDVVLKGADIDMDRLPVPICWPQDGGPY
                     ITLPQVVTRDPATGARNVGMYRVQVLGRDSLAMHWQRHKVGAAHWREMAARGETMPVA
                     IVLGGDPASIYAASAPLPPGIDEYLFAGFLRREPVRLSKCVTSDLEVPSEAELVIEGV
                     IDPREPLVTEGPFGDHTGFYSLADLYPRVQVTAITRREDPVWPHTIVGRPPMEDFWLG
                     HATERIFLPLLRLTLPEVVDMHMPAEGIFHNLVFVSIDKQFPGQAYKVMNGLWGQGLM
                     SLAKVIVVVDKDVNVRDPKEAWWVALNHIDPERDVRFSMGPIDVLDHASREFTYGSKM
                     GIDGTRKWKDEGFHREWPDKIVMDEETKAKVDAMWRELGISIP"
     gene            5365..6264
                     /locus_tag="VFU75_05870"
     CDS             5365..6264
                     /locus_tag="VFU75_05870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA-like polyprenyltransferase"
                     /protein_id="HEU5466397.1"
                     /translation="MASPSREGQTLGGDSLLVRYANFVRLPHTVFALPFALIGAVAAS
                     WTSPVTWRQAVLVVIAFTAARFVAMGFNRIADRDLDARNPRTASRDIPQGRISVREAW
                     VSVTLAALVFEGSAWALNSLCFALSPIALVAITGYSYTKRFTWSSHLWLGLADAISGP
                     AGYLAITGKWSEPWWVLPVLALVVTAWVGGFDMFYALQDEEFDRKEGLHSAVVRFGAG
                     PAIFLAKTLHGLAILALVAFGLGAGFHVPFYVGVGIGGGLIAWEHQLVKPGDLSKLNA
                     AFFTMNGIISITVFLGALVDRVI"
BASE COUNT          905 a         2059 c         2142 g         1158 t
ORIGIN      
        1 tgaaagagac cccggccccg tgagtgaggc cgtagcgcct tccgggcccg ccgggaagct
       61 caccaccgga gatcttcgct ccaccatcct gcgcctcgcg gcacccaccg tggcgatgat
      121 ggcgttcaat accggcttca acgtcatcga ctcgatctgg gtaggtcagc tgatcggacc
      181 cgcggcgctc gccgcggtgt cgactgccgg gttcacggtt tgggtgatga tgagcctggc
      241 cgaaatgctg gacgtgggtg tggtggcctt ggcggcacac cgccatgggg cccgcgcacc
      301 cgaccaggcg gcgcgcgtgg cggcagctgc gctcgtgacc ggcctggtcg cggggctcct
      361 tgcaagcttc gccggactgg ccgcgctccc caagctcttc gacttgatgc aggtcccgcc
      421 cgaagtcgcc gtcctggggc acacctatct gcacacttgg ctcctgggcg cgcccctcgt
      481 cttcgggttc ttcgcgctcg aggcgacgtt ccgctcgtcc ggggacacga cgaccccgtt
      541 catggtgctg gccgcatccg ccgtcgtgag cgtgattctc gaccccctcc tcatcgccgg
      601 gatcgggcca ttcccgaggc ttggcgtcga gggggccgcg ctggcatcgg tcatggtgcg
      661 cggcgtggcg tgtctcgcgc tggtgtggct cgcctttcgg cgcggtcaca tccacttccg
      721 ggcgcccgac tgggcatccg cgccccacat cctgcggatc ggccttccgt actcgatgag
      781 cgggattctc ttctcgctcg tctacatgtg gctgacgcgg ttcacctcac gcttcgggac
      841 gccggcgctc gcggcgctgg gggtgggaca taaagtggaa ggcgtagggt tcgtggccat
      901 caccgggttc acgctcgccg ccgcggcatt ggtggggcaa agcctcggcg ccaaggatga
      961 ggcacgagcc cggaaggcgg cctggcacac ggtgggctac gccctgctgg tgacgacctg
     1021 taccgcgctc gcctgtctcc tggtgccccg gttcttcgtc cggctcttca cgtccgatcc
     1081 gcaggtcatc gccaacggga cgggctacct tcggatcatt gcgatggcgc agataggcca
     1141 gaccttcgag ctgattctcg aaggcgcgct cgccggcgcg ggctatacat tctggcccat
     1201 ggtgtggagc tcgagtctga ccttcgcgcg aattcccctc gccgcctggt ggtcggggat
     1261 ctggggtgtc acggggatct ggtgggctat ttccctcacc gcgaccctgc gcggcgtcgc
     1321 gatgaccgtg ctgtggctgt cgggcgcctg gcgccccgta ccgaggaccg cgtgaactcg
     1381 atcattacgc tcctgacgga ctacggcgcg ggggacagct acgtcgccga ggtgaagggc
     1441 gtcatcctga ccgcggtgcc cgacgccaca ttgatcgacg tgacccacca ggtatctccg
     1501 ggggacgtcc gggccgggca gttcctgttc aacagagcct ggcggcgctt tccggccgga
     1561 accgttcacc tcgtggtggt ggaccccggt gtcgggacgt cacggcgggc gctcgccgcc
     1621 gaagcgcacg gccacttctt cgtcgccccc gacaacgggc tgctcacgtc actgcccgag
     1681 gacgcggaat tcgtctccct ccccgtgccg ccgtcggcat cgccgacctt tcatggccgc
     1741 gacgtcttcg ctcccgccgc ggcgcgtctc gcgatggggg tgtccctcga cgagcttggc
     1801 gatcccgtgg acgacgtccg ccggtcacca ctgccggagc ctcgcgcgga tggcgtcgcg
     1861 ctcctgggcg aagtgatcta cgtcgaccgc ttcggcacgc tggtctcgaa catcagagcc
     1921 gaggacgtcg atgagggagt gcggatcacg gtggagggga atgacgtcgg cgcgttgcgc
     1981 cggactttcg gggatgtgga gcccggccag ctggtcgcgt acacgggcag cggcggggag
     2041 gtggagatcg ccgtccgcgg cggtagtgcg gcccggctcc tgggagtggg cgtgcgggcg
     2101 gaggtgaggg cctgatcgta cccaccctga aagggcgggc ccggaaaagc gctgcgcttg
     2161 aagcgctgtg ctctgtcggg ccccggcttc agccggggtt cgacgagtga tgggctttcc
     2221 ctgaagacac gagggcggct ggggagtcac acccctgccg ccctcgtcag ttaccgggac
     2281 cctggaaccg gtcagccgtg atggctgccc gaaggggtcg gaatccggac ctgtgccagc
     2341 accttctcgg cgaagtccgc cgagggggcg aaccgctgga gctgcgccag ctcgacgagc
     2401 agcttgcgct cgcgctccgc cactgcgagg cactcggcac attccgtcag atggaacgtg
     2461 cgggccgcgc tcaggaggcc ggtggcccag agctcgactt cctccggggt cagatgggtc
     2521 tgaagtctct cgttcatacg gtctccatac gggggcccgt aatgggtggt ttcactcccg
     2581 ggcgtcctcc agatagaccc gcaactcatt acgcgcccga tggatatacg ttttcaccgt
     2641 gccgagcgga aggtcaagca tctgggcgat ttcttcgtag gaatggccct ccacgtggcg
     2701 gagcagaatg cacgaccggt actcgggccg gaggcggcca atggcccgtt cgatggcgcc
     2761 ccccaactcc cgggactcga cctcatcgag cgggctctcg gatacgtcgc cgagctgcgg
     2821 ggcactcgcg tgcatctctt cgggcgagcg ggcggtgggg gagccgtcga gggacagggt
     2881 gtcgagctgc cggcgccgca ggtgatcgat ggccgcgttg ttggcgatct tgaaaatcca
     2941 cgacgagaac ttgtactcgg gcttgtagct cttgagggcg ttgaggacct tgatgaaggt
     3001 ctcctgggcc agatcctcgg ccagcgcgcg gtcacgcacc atccggtaaa tgagcgaaaa
     3061 caccggccgt tcataccggt ggaccagctc gcgaaacgcg ggctcgtgtc cgtcgcgagc
     3121 ccagcgcacc acgtcctggt cgctggcgcc cgtcaggtcg ttcggcctat cttttccgcc
     3181 tatggcgccg tctcctcagt caaatgtgat gcgcgcgggg ggaggggcag aaggcaagcg
     3241 ctcgtacgtt cgcgatgtgt tcaccgcgat cgccccccgg tacgacttcc tcaatcacct
     3301 gctcagcctc aacgtcgacc gccgctggcg ccgccgcgcc gtggcgcggc tgggctggga
     3361 acggcgtccc gacggcctct atctggatct ctgcgccggt acgctcgacc tcgcggcggc
     3421 cctcgcctcc gccgagggct tccgcgggcg gatcatcggg gccgacttcg tgattcccat
     3481 gctgcagcgg ggtcgcggca aagccgcggc ggtccgcccg gtcggcgccg acgccctgga
     3541 gctgccgttc cccaatgagg gattcgacgg cgcgctggtg ggcttcgggg tgcgcaatct
     3601 cgcggacctg gacctcggac tctccgaagc ccggcgggtc ctcaaaccgg gagcccggct
     3661 ggtcatcctg gaattcacca ctccgcgttt cgcgcccctc cgcgccgcct acctcttcta
     3721 ctttcagcga ctgctgccgc tgatcggccg cacggtgtcg aaacatacgg acgcctatac
     3781 ctacctgcca gagtcggtgc tcaactttcc cgatcccgac gccctttccc gtcgtctgga
     3841 aaccgctggt ttctcggacg tgcgctacga actcctgacc ggcggtatct gcgcgatcca
     3901 ccatggcact cgataacctg cgggacttcc tggcggccct cgacgaggcg ggcgagctgg
     3961 tgcgcgtgca ccggccagtc ctcgtcgatc gcgaaatcac ggagatcgcc gaccggtgca
     4021 tgaagtcacc aggcggggga ccggccttgc tgttcgagcg cccgaccttg acctcgggtg
     4081 gtgcatcccc tcaccccgtc gccgtcaatc tcttcgggtc ccaaaagcgc atgtgcatgg
     4141 cgctcggcgt cgcgaagctg gatgagatcg gtgaccgggt ttcggagttc gtgaacctca
     4201 aggtcccgga cggcctcgtc ggcaagctcg cgatgctccc caaactggcc gaactgacca
     4261 agttcccccc gcgcagtggc gggggaagtc cggcgtgcca ggatgtggtg ctcaagggag
     4321 ccgacatcga catggaccgt ctgccggtcc cgatctgctg gccgcaggat ggcggaccgt
     4381 acatcacgct cccgcaggtc gtcacccgcg atccggcgac gggcgcccgt aacgtcggga
     4441 tgtaccgggt gcaggtgctc gggcgcgact cgctcgccat gcactggcag cgtcacaagg
     4501 tcggtgcggc acactggcgc gagatggcgg cccggggtga aaccatgccc gtcgcgatcg
     4561 tcctgggtgg cgacccggcg agcatctacg ccgcctcggc gcccctgcct ccggggatcg
     4621 atgagtatct cttcgccggc ttcctgcgcc gggagccggt gcggctctcc aagtgcgtca
     4681 cctcggatct cgaggtgccg tcggaagcgg agctcgtcat cgaaggggtg atcgatccgc
     4741 gggagccgct cgtcaccgag gggcccttcg gcgatcacac cgggttctat tccctcgccg
     4801 atctttatcc aagagttcag gtcacggcga ttacccggcg ggaagatccg gtgtggccgc
     4861 acacgatcgt cgggcgtccg cccatggaag acttctggct cggccatgcc acggagcgga
     4921 tcttcctgcc cctgctgcgg ctgacgctcc ccgaagtcgt cgacatgcac atgccggccg
     4981 aggggatctt tcacaacctc gtgttcgtca gcatcgacaa gcagttcccg gggcaagcgt
     5041 acaaggtgat gaacggtctc tgggggcagg ggctgatgtc gctcgccaag gtcatcgtgg
     5101 tggtggacaa ggacgtgaat gtccgcgacc ccaaggaagc gtggtgggtc gccctcaatc
     5161 acatcgatcc ggagcgggac gtccggttct ccatggggcc gatcgacgta ctcgatcacg
     5221 cgagccgcga attcacgtac ggctcgaaga tggggatcga cggtacgcgg aagtggaagg
     5281 acgaagggtt ccaccgcgag tggcccgaca agatcgtgat ggacgaagag accaaggcga
     5341 aggtggacgc gatgtggagg gagcttggca tctccatccc gtgaggggca gaccctcggc
     5401 ggtgactcac tgttggtccg ctacgccaat ttcgtcaggc tgccgcacac cgtcttcgcg
     5461 ctcccctttg cgctgatcgg cgccgtcgcg gcgtcatgga cgagcccggt gacctggcgt
     5521 caggccgtgc tggtcgtgat cgccttcacg gcggcgcgct tcgtggcgat ggggttcaac
     5581 cggatcgccg accgcgacct ggacgccagg aatccgcgca cggcttcccg ggacattccc
     5641 cagggccgga tcagcgtccg cgaggcgtgg gtgtccgtga ccctcgccgc cctcgtgttc
     5701 gagggctcgg cttgggctct caattccctc tgctttgcgc tgtcccccat cgcgctcgtc
     5761 gccatcaccg ggtacagcta taccaagcgc ttcacgtggt cctcgcatct ctggctcggg
     5821 ctggcggatg ccatctcggg gcccgcgggc tacctcgcga tcacggggaa gtggagcgag
     5881 ccctggtggg tgctgccggt gctggcgttg gtggtgaccg cgtgggtcgg tggcttcgac
     5941 atgttttatg cacttcagga tgaggagttc gaccggaaag agggactcca ctcggcggtg
     6001 gttcgattcg gtgcggggcc ggcgatcttc ctggcgaaga cgttgcacgg gctggcgatt
     6061 ctggccctgg tggccttcgg gctcggcgcc ggattccatg tgcccttcta cgtcggcgtg
     6121 gggatcggtg gggggctcat cgcgtgggag catcaactgg tgaagccggg cgatctctcg
     6181 aagctcaacg cggcgttctt tacgatgaac gggatcatca gcattacggt gtttcttggt
     6241 gcgctcgtgg atcgggtgat atga
//
LOCUS       DASUBP010000160        11629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_890226, whole genome shotgun sequence.
ACCESSION   DASUBP010000160 DASUBP010000000
VERSION     DASUBP010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 11629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11629
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_890226"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..458
                     /locus_tag="VFU75_05875"
     CDS             <1..458
                     /locus_tag="VFU75_05875"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit I"
                     /protein_id="HEU5466398.1"
                     /translation="LRATAQGMMLTASHIFRPKVTMQYPEEKGSHDWTMSSRWRGTHR
                     MLTDEQGRAKCVACGLCPQICPANCIKLVPGEDEQGNRYPLIYEIDEFRCVFCGYCQE
                     VCPEEAIHVGWHYENAEYSRDRFVYDLERLCAQTHPVSTLWDPADPKGE"
     gene            455..973
                     /locus_tag="VFU75_05880"
     CDS             455..973
                     /locus_tag="VFU75_05880"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HEU5466399.1"
                     /translation="MNLVTIVFWIFAGLALGSGVLCVTRRSPVASALWLVVTMFSLAV
                     LFVLLDAQFIAVLQVLVYAGAVMVLFLFVIMLLNLGRGAGQDRKGVVGLTVGAVLAGI
                     LFIQLRFLWNAAPPALIQVPNVMAQLQQQHGMVGSVAGPLYDAYLVPFEITSVLLLAA
                     VVGAVVLAKRKL"
     gene            974..1270
                     /gene="nuoK"
                     /locus_tag="VFU75_05885"
     CDS             974..1270
                     /gene="nuoK"
                     /locus_tag="VFU75_05885"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HEU5466400.1"
                     /translation="MLLGPSLAVAAVLFVIGVLGVLIRRNAIIIFMCVELMLNAVNLT
                     FIAFAQSLGVGGQIFVFFVMAVAAAEAAVGLAIVLAIYRHKETVDLRNINILKG"
     gene            1283..3247
                     /gene="nuoL"
                     /locus_tag="VFU75_05890"
     CDS             1283..3247
                     /gene="nuoL"
                     /locus_tag="VFU75_05890"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HEU5466401.1"
                     /translation="MQLVWLVPLLPLLGFVLNGTIALTRPQAKTAVSLIGPGVLLAAF
                     GVAVAVVKDLAAAHSGTPAVFTYWDWIPVGALHISAALQVDPLSAVMILIVTGVGSLI
                     HIFSVGYMRSDEGYARFFAYLNLFVFFMLVLVLGASFPVMFVGWEGVGLCSYLLIGFW
                     FDVTANADAGKKAFIMNRIGDFGFLAAMFLIFQATGSLDFTSVFSAAPSTFAPGSGTV
                     TAITLFLFLGCAGKSAQIPLYTWLPDAMAGPTPVSALIHAATMVTAGVYLVARANLLF
                     ALAPVSSAVVAGIGALTALFAATIALKQWDIKKVLAYSTVSQLGYMFVGVGTGAYAAG
                     VFHLATHAFFKALLFLGSGSVIHAMHGAYHHTHAHDDAQDMRNMGGLRKYLPWTWALM
                     WIATLAIAGIPPFAGFFSKDEILGAAFARGGAQPIWYVFWAFGLIAALLTAFYMTRMM
                     LYTFHGPNRTGEKEREHLHEAPWVMTGPLVVLGILSLAGGVLNLPALIGGNSWLEHWL
                     EPVTAGSASLLPAAEVSQGTEWALLAVAVAIALTGIIGAIQLLKPAALVPARQAPAEH
                     GLGKVLANKWYVDELYDAVVVKPLGWLSRVVLWRGVDQGLIDGAGVNGSAAVSRALGW
                     VGSKLQTGEVGFYVVLFVLGVALVLRAAIR"
     gene            3244..4752
                     /locus_tag="VFU75_05895"
     CDS             3244..4752
                     /locus_tag="VFU75_05895"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HEU5466402.1"
                     /translation="MNSYDVWVLPVLLAWPLVGAALLYLVPASRAKVLALVVSLIEFG
                     ISVPLWWRFVPAGGMQFGVNWNWIPAWGIQFHLALDGISLFMVLLTTFLVPLSVLGSW
                     TYITKRERGFYALLLVLTTGMLGVFVSLDLFLFYVMWEVMLIPMYFIIGIWGGENRIY
                     AAIKFFIYTFLGSLLMLVAILGLYLHAAKTTGQYSFDYDFLLQRSADLGPVAFWFFAA
                     FFLAFAIKVPMFPLHTWLPDAHVEAPTAGSVILAGVLLKMGTYGFLRFALPFFPQIAM
                     SKTVVAVVVTLSVVGIIYGGLVAMVQPDFKKLVAYSSVAHLGFVMLGIWALTLQSVQG
                     ALLVMINHGISTGALFFLAGMLYERRHTRLLDAFGGLARVVPLLAAALTIVSLSSVGL
                     PGTNGFVGEFLILIGAFKTYPVAAIISTTGVIVAAMYLLPALQKIIYNPLAKPENQKL
                     LDLSPRELIVLLPLLAAIIWIGVYPRPFLSRMEPAAQHLIQTVTPATTAAAR"
     gene            4749..6302
                     /locus_tag="VFU75_05900"
     CDS             4749..6302
                     /locus_tag="VFU75_05900"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HEU5466403.1"
                     /translation="MSLDLSNYADLMRALSPESVLTILAMLVLLIVAWRHRTVTDLRI
                     AGWVTLVSIGITAAVVWWLWWNSATVEGVAQMIAVDDFRFVTDWIFLGAAALTVLFSF
                     RYLEREQLLAPEYYPLLLFAVLGMMLMGGGEDLMVIFLGLELMSISVYVLAGFNRRSP
                     RAAEAALKYFLLGAFASAFLLYGMALIYGATGTTNLSFIGVQVQTLGVAHLPLLLVGI
                     GLLLVGFGFKVAAVPFHMWAPDVYDGAPTPITGFMATGVKAAAFAALFRIVIESLGAT
                     ASWHLIIAGLAVATMIGGNLIALAQRSVKRMLAYSSVAHAGYLLVAVVSGGSGAGAFI
                     FYLVAYGLTTIAAFALLAAKGKGGESDVRVDDLSGLSTSRPWLALALTVCMLSLLGFP
                     GTAGFMGKWYILVSAVGGGHTGLAIILVLTSVASAGYYLPVVMAMYMKPASHAEAHSS
                     MVFDGVGRATLAVAVAAILWFGIAPGQLFDLSKTSGASLHAAPAMAMDSPTPTPTTTA
                     SGTGATTAR"
     gene            6326..7705
                     /locus_tag="VFU75_05905"
     CDS             6326..7705
                     /locus_tag="VFU75_05905"
                     /inference="COORDINATES: protein motif:HMM:NF014877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEU5466404.1"
                     /translation="MRIPQTIFRQYDIRGIVGEQLTPGVAHAVGQAFATLARARLSRA
                     PVLAVGRDNRPSGPELARHVRAGIVAAGGRAVDVGECPTPALYFATHTLKVDGGLQVT
                     GSHNPPEFNGFKMVVVGEAVAGEQIQDLYRMIAGDSLDRAAGGSEATHQGLLSAYREA
                     IVSRNTPLARPLKAVVDCGNGVTSLIAIETLRQLGVTVVPLFAESDGRFPNHHPDPTV
                     VENLRDLQAAVVKEKAELGIAFDGDGDRIGAVDEAGTPIFGDQLLVLFGRDLAKRQPG
                     KHPVIFDVKCSEVLPQMLIKAGLEPVMWKTGHSLIKQKMKDLHAPLAGEMSGHMFFGG
                     DWFGFDDALFGAARLLHYVAREGGPLSRLLADLPRTVNTPELRVDCPDERKFDVVERA
                     AKYFGAKYPVSTLDGVRMTFPTGWGLIRASNTQPVLVMRFEATSATDLERYRAEVEGW
                     LAQNAGGPG"
     gene            7702..10128
                     /locus_tag="VFU75_05910"
     CDS             7702..10128
                     /locus_tag="VFU75_05910"
                     /inference="COORDINATES: protein motif:HMM:NF015644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0182 family protein"
                     /protein_id="HEU5466405.1"
                     /translation="MTRRRWALLGAAALLVVVLVGGRWFAMQTAEHAWAATIPNGAAY
                     LRTLALARTVQWIVALAAVLWGMGHCYGVYRAIGSVQMPRRLGDIEIVEAVPHRLLIV
                     VTLVGGVVYGGALAWAIGDVWRPALLASAPPHFGLTDSVLHQDLGYYVGELPWARTRQ
                     GMLLIASLTASFLIAVLYVAIGSIRMHGGRIAASPHARAHLGLTLAALAVAFVWGAHL
                     DPYETITGLVGPVTHDAIAIRGPGASAVTVVALITAFVSAVWGWWDRPGVVGATWLAL
                     LVTMLAAYVILPPATHTIGTPSPADRALQDDKGTLEGVAFGAAPESWNLPSLSPAALV
                     ASIPVWDPGRIRIRALRDSAVGQGLSVYNMTLEPSGRWLLGLAPDGTNLLRVSPPPTW
                     EQVHQSVWAHAPGPLEARETDSTLTFARAPGGPRATWFGGGFLDYAVVDSGSMTGIPL
                     TGTWRRLALAWTLQAPQLAQVASRGRTLVWRRDAVERFEALAPFARFEMPAPVLVGDS
                     LWWISWGSVEADAFPLVDTVMIDGRTIRYHRSGLVGALNAVSGSTRLWLAPQTDSLTA
                     AWARIVAPLVAPADSMPPALRRVLPYPADAFEIQARILARTIPLPDSTYRWLPLVAEH
                     PFTLAAPGPDSATDVWYAQAFVDSTRQSTFGGILAGTMTEKGPRLFFWTPKPSTIPTP
                     LSSDRERRSGVERVWPTDVGVMTVQARFLEPEGSPVPAKLDSVYVSLGPDVAADVSAP
                     AALKTLLRGPDLGEGTPTASLRRARALLARMDSALAAGHVARFGVLYDSLRALLRGTS
                     RGVAPSTGPR"
     gene            10140..11114
                     /locus_tag="VFU75_05915"
     CDS             10140..11114
                     /locus_tag="VFU75_05915"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEU5466406.1"
                     /translation="MTGTGVRVKDVIVVALGGNAVAPQGERPTIANQFRHTRQSLAPI
                     VELARAGWGIALVHGNGPQVGDELARNELAADQLEPLPLGVLVAATAGWMGYMIQQSL
                     QNALRRAGVERQVVTMVTQTICDASDPDLRRPTKPIGHALDAATRQRLEARGQAVRED
                     RPGRARRVVPSPKPIGVVERKMIADLVSRGHIVVACGGGGPPVYEDPKLGIEGLDAVV
                     DKDRVAAVLGAELKAKALLILTDVDAVYAHFGTAHQERLGKLSLRDAERLLAGDELGS
                     GSMRPKVEAAADFVRAGGKGARAVVAELSKGLEALAGQSGTTITRENG"
     gene            11111..>11629
                     /locus_tag="VFU75_05920"
     CDS             11111..>11629
                     /locus_tag="VFU75_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU5466407.1"
                     /translation="MNLHEYQARDILRKHGIPMPEGAVARTPAEVKAIAQKLGGTVVI
                     KAQVHAGGRGKAGGVKLAKTPAEAELLASKILGMQIKGLTVEKVLVVPAADIVSESYV
                     GFIVDRATQRPVLMVSAAGGVDIEEVAAKTPEKIHRLAVDPRYGLLPHQAQGLAFHLY
                     QDVAQVRQAADLM"
BASE COUNT         1614 a         3934 c         3935 g         2146 t
ORIGIN      
        1 acctccgggc gaccgcccaa ggcatgatgc tcaccgcgtc ccacatcttc cggcccaagg
       61 tcacgatgca gtatccggaa gaaaagggca gccacgactg gacgatgtcc agccggtggc
      121 gcgggacgca ccgcatgctc accgacgagc agggccgcgc gaagtgtgtg gcgtgcggcc
      181 tctgccccca gatctgcccg gcgaactgca tcaagctggt gcccggtgag gacgagcagg
      241 ggaaccggta tcctctgatc tacgaaatcg acgaattccg ctgcgtgttc tgcggctact
      301 gccaggaagt ctgccccgaa gaggcgatcc acgtgggctg gcattacgag aacgcggagt
      361 actcgcggga tcgcttcgtc tacgatctgg agcgcctgtg cgcccagacc cacccggtgt
      421 ccacgttgtg ggacccggcc gatccgaagg gcgaatgaac ctcgtcacga tcgtgttctg
      481 gatcttcgca gggttagccc tcggttcggg cgtcctgtgc gtcacgcggc ggagcccggt
      541 ggccagcgcg ctgtggctgg tagtcaccat gttctcgctc gccgtgctct tcgtgctgct
      601 cgacgcccag ttcatcgccg tcctccaggt cttggtctat gccggcgcgg tgatggtgct
      661 cttcctgttc gtcatcatgc ttctgaacct cgggcgtgga gcggggcagg accgcaaagg
      721 ggtggtcggg ctgacggtcg gcgcggtgct cgcgggcatt ctgttcatcc agctcaggtt
      781 cctgtggaat gccgccccac cggcgctcat ccaggtgccg aacgtcatgg cccagctgca
      841 gcagcagcat ggcatggtcg gatcggtggc cgggccgctc tatgacgcct atctcgtgcc
      901 cttcgaaatc accagcgtgc tcctcctcgc cgccgtggtg ggcgcggtcg tgctcgcgaa
      961 acggaagctc tagatgctcc tcggacccag cctcgcggtc gccgcggtgc tcttcgtgat
     1021 cggcgtcctc ggcgtcctca tccgccggaa cgcgatcatc atcttcatgt gcgtcgagct
     1081 gatgctcaac gccgtgaacc tgacgttcat cgccttcgcg cagtccctcg gcgtcggcgg
     1141 ccagattttc gtgttcttcg tgatggccgt cgctgccgcc gaagcggcgg tcgggctcgc
     1201 catcgtcctc gcgatctacc gccacaagga gacggtggat ctgcggaaca tcaacattct
     1261 gaagggctga ggccgcctcc cgatgcaact ggtctggctg gtcccactcc tgcccctcct
     1321 cggcttcgtg ctgaacggca cgatcgccct gacgcgcccc caggccaaga cggcggtgtc
     1381 gctgatcggc cccggggtgc tgctcgcggc cttcggggtg gccgtcgcgg tggtcaagga
     1441 tctggccgcc gcgcactccg gaacccccgc ggtctttacc tattgggact ggatcccggt
     1501 gggggcgctg cacatctccg ccgccctgca ggtggacccg ttgtccgcgg tgatgatcct
     1561 gatcgtgacc ggcgtgggct cgctgatcca catcttcagc gtcggctata tgcgctcgga
     1621 cgaggggtac gcgcggttct tcgcgtatct caacctgttc gtcttcttca tgctggtgct
     1681 ggtcctggga gcaagcttcc cggtgatgtt cgtgggctgg gagggggtcg gactctgctc
     1741 gtacctcctc atcggcttct ggttcgacgt caccgccaac gcggacgccg gcaagaaggc
     1801 gttcatcatg aaccggatcg gggacttcgg cttcctcgcc gcgatgttcc tgatcttcca
     1861 ggccacgggg tccctcgact tcacatccgt gttctccgcc gcgccgagca cattcgcgcc
     1921 gggaagcggg acggtcacgg cgatcacgct tttcctgttc ctcgggtgcg cggggaagtc
     1981 ggcccagatt ccgctctata cctggctgcc tgacgccatg gcgggtccga cgccggtctc
     2041 ggccctgatc cacgccgcca ccatggtgac ggcgggcgtt tacctggtcg cccgggcgaa
     2101 cctcctcttc gcgctggcgc ccgtctcgtc cgcggtcgtc gccgggatcg gcgccctgac
     2161 ggcgctcttc gcggcgacca tcgcgctcaa gcagtgggac atcaagaaag tgctcgcgta
     2221 ctccactgtc tcgcagctcg gctacatgtt cgtgggcgtg gggacggggg cctacgccgc
     2281 cggcgtgttc cacctggcca cgcacgcctt cttcaaggcg ctcctgttcc tgggttccgg
     2341 cagcgtgatt cacgccatgc atggcgccta tcatcacacc catgcccacg acgacgcgca
     2401 ggacatgcgc aacatgggtg gtctgcggaa atacctgccc tggacctggg cgctgatgtg
     2461 gatcgcgaca ctcgccatcg ccggcatccc gccctttgcc gggttctttt cgaaggatga
     2521 gatcctgggc gcggcgttcg ctcggggtgg cgcgcagccc atctggtacg tcttctgggc
     2581 gttcggcctg atcgcggccc tgctcacggc gttctacatg acccgaatga tgctgtacac
     2641 gttccacggg ccgaaccgga ccggggaaaa ggagcgggaa catctccacg aggctccttg
     2701 ggtgatgacc ggaccgctcg tggtgctcgg cattctgagc ctcgcgggcg gagtgctcaa
     2761 tctgccggcg ctgatcggcg gcaattcgtg gctcgagcat tggctcgagc cggtgaccgc
     2821 cggatcggcg tcattgctgc cggccgcgga ggtttctcag ggcaccgagt gggcgctcct
     2881 cgccgtggcg gtggcgattg cgctcaccgg tatcatcggg gcaattcagc tgctgaagcc
     2941 cgcggcgctg gtgccggccc gccaggcgcc cgccgagcat gggctgggga aggtgctcgc
     3001 gaacaagtgg tacgtggacg agctgtatga cgccgtggtg gtcaagccgc tcggctggct
     3061 ctcccgtgtg gtgctgtgga ggggagtgga ccagggactg atcgacggag ccggggtgaa
     3121 tggctccgcc gcggtctcgc gcgccctggg gtgggtgggg agcaagctcc agacgggcga
     3181 agtcggcttc tacgtcgtcc tcttcgtgct gggcgtcgcc ctcgtgctcc gggcggccat
     3241 ccgatgaact cctacgacgt ttgggtactg ccggtgctcc tggcgtggcc cctggtgggc
     3301 gccgcgctcc tgtacctcgt ccccgcgtcc cgcgcgaagg tgctggcgct cgtcgtcagc
     3361 ctcatcgagt tcggcatctc cgtaccgctc tggtggcggt tcgtgccggc gggcggcatg
     3421 cagttcggcg tgaactggaa ttggattccc gcctggggca tccaattcca cctggcgctc
     3481 gacggcatct ccctgttcat ggtcctgctg acgacgttcc tggtgccgct ctcggtgctc
     3541 gggagctgga cctacatcac caagcgggaa cgtgggttct acgcgctgct cctggtactc
     3601 accacgggca tgctcggcgt cttcgtctcg ctcgacttgt tcctcttcta cgtgatgtgg
     3661 gaagtgatgc tgattccgat gtatttcatc atcgggatct ggggcgggga aaaccgcatt
     3721 tacgcggcga tcaagttctt catctacacc ttcctcggct cgcttctgat gctggtcgcg
     3781 atcctcggcc tgtatctgca tgccgccaag accaccggcc agtattcgtt cgactacgac
     3841 ttcctgctcc agcggagcgc cgacctggga ccggtcgcct tctggttctt cgccgccttc
     3901 ttcctggcct tcgcgatcaa ggtaccgatg ttcccgctgc acacctggct gcccgacgcc
     3961 cacgtggagg caccgacggc gggctcggtg atcctggcgg gagtgctgtt gaagatgggg
     4021 acctacgggt tcctccggtt cgccctgccg ttcttcccgc agatcgccat gagcaagacg
     4081 gtcgtggcgg tggtcgtcac cctgtcggtc gtcggcatca tctacggcgg cctcgtcgcg
     4141 atggtgcagc ccgacttcaa gaagctcgtg gcctattcgt cggtcgcgca cctgggcttc
     4201 gtgatgctcg gtatctgggc gctgacgctc cagagcgtgc agggagccct gttggtgatg
     4261 atcaaccacg gcatctccac cggcgcgctg ttcttcctgg ccggcatgct ctacgagcgg
     4321 cgccataccc gcctgctcga cgcgttcgga gggctggctc gtgtggtgcc gttgctggcc
     4381 gccgccctga ccatcgtgtc gttgtcgtcg gtgggattgc cggggaccaa cggattcgtg
     4441 ggtgaattcc tgatcctgat cggcgccttc aagacctatc ccgtggcggc gatcatctcc
     4501 accacgggcg tcatcgtcgc ggcgatgtac ctgctcccgg cgctgcagaa gatcatctac
     4561 aacccgctcg ccaagccgga gaatcagaag ctgctcgatc tctcgccgcg ggagctgatc
     4621 gtgctgctcc ccctcctggc ggccatcatc tggatcggcg tgtatccccg cccgttcctg
     4681 agccggatgg aacccgccgc ccagcatctg atccagaccg tgactcccgc gaccaccgcg
     4741 gcggcccgat gagcctcgac ctctcgaact acgccgacct gatgcgggcg ctctccccgg
     4801 agagcgtgct caccattctg gccatgctgg tgctgttgat cgtggcttgg cggcatcgca
     4861 ccgtgaccga cctgcgcatc gccggatggg tgacgctcgt gtccatcggg atcacggccg
     4921 ccgtggtctg gtggctctgg tggaacagcg cgacggtcga gggcgttgcg cagatgatcg
     4981 ccgtcgacga cttccgcttc gtcaccgact ggatcttcct cggggccgcc gcccttacgg
     5041 tgctgttctc gttccggtat ctcgagcgcg aacagctcct cgcacccgag tactatccgc
     5101 tcctcctgtt cgccgtcctg ggcatgatgc tgatgggggg cggggaggat ctgatggtca
     5161 tcttcctcgg cctcgagctg atgtcgattt ccgtctacgt cctcgccggc ttcaaccgcc
     5221 ggagcccccg cgcggcggaa gcggccctga agtacttcct gctgggcgcg ttcgcctcgg
     5281 cgttcctgct ctacggcatg gccctcatct acggtgccac cggaacgacc aacctctcgt
     5341 tcatcggtgt ccaggtgcaa accctgggcg tggcgcacct gccgctgctc ctggtgggta
     5401 tcgggctctt gctggtcgga ttcggattca aggtcgccgc cgtaccgttc cacatgtggg
     5461 ccccggatgt ctacgacggc gcgcccacgc ccatcacggg cttcatggcg acgggggtga
     5521 aggcggccgc cttcgcggcg ctcttccgca tcgtgatcga atccctgggc gccaccgcga
     5581 gctggcactt gatcatcgcg ggcctggccg tggcgacgat gatcggcggc aacctgatcg
     5641 cgctcgccca gcggtccgtg aagcggatgc tggcgtactc gtccgtcgcg cacgccggct
     5701 acctcctggt cgccgtggta tccggcggct ccggtgcggg tgcgttcatc ttctatctcg
     5761 tggcctacgg cctcacgact atcgccgctt tcgcgctcct cgccgccaag gggaagggcg
     5821 gggagtccga cgtccgggtc gacgatctgt cggggctgtc gaccagccgt ccctggctcg
     5881 cgctcgcgct caccgtgtgc atgctctccc tgctcggatt cccggggacc gccggattca
     5941 tggggaagtg gtacatcctc gtatccgccg tcggcggggg acacacgggg ctcgcgatca
     6001 tcctcgtgct caccagcgtc gcatccgcgg gctattactt gccggtcgtg atggccatgt
     6061 acatgaaacc cgcgtcccat gccgaagccc actcgagcat ggtgttcgac ggcgtcggac
     6121 gggccacgct ggccgtcgcc gtggcggcga ttctctggtt cgggatcgcc ccgggacaac
     6181 tgttcgacct gtccaagacg tcgggggcga gccttcatgc cgcgcccgcc atggcaatgg
     6241 actcgccgac cccgacgccc acgacgacgg cttccggcac tggggccacg acggcccgct
     6301 gacacctccc cgggaccccc cggtcatgcg cattcctcag accattttcc ggcagtacga
     6361 catccgtggc atcgtcgggg agcagctcac gcccggcgtt gcccacgcgg ttggtcaagc
     6421 cttcgcgacc ctggcacggg cccggttgtc gcgagccccg gtgctcgccg tcgggcggga
     6481 taaccggccc tcggggcctg agctcgcccg tcacgtccgc gccggcatcg tcgccgccgg
     6541 cggccgtgcg gtggacgtcg gcgagtgtcc gactcccgct ctgtatttcg cgacgcacac
     6601 cctcaaggtg gatgggggcc tgcaggtcac cgggtcgcat aacccgccgg agttcaacgg
     6661 attcaagatg gtggtcgtgg gagaggcggt cgccggcgag cagattcagg acctctaccg
     6721 catgatcgcc ggagactccc tcgatcgggc cgctggtgga tccgaggcga cgcaccaggg
     6781 actgctctcc gcgtatcggg aggcgatcgt cagccgcaac acgccgctcg cccgtcccct
     6841 caaggccgtg gtggattgcg gcaacggcgt caccagtctg atcgccatcg agaccctgcg
     6901 gcagctcggg gtgaccgtcg tcccgctctt cgccgagtcc gacgggcggt tccccaatca
     6961 ccaccccgat ccgacggtgg tcgagaacct ccgcgacctc caggcagccg tcgtgaagga
     7021 gaaggcggag ctggggatcg cgttcgacgg ggacggggac cgcatcggtg cggtggacga
     7081 agccgggacg ccgattttcg gtgaccagct gctcgtgctc ttcggccggg atctggcgaa
     7141 acgccaaccg ggcaagcatc cggtgatttt cgacgtgaag tgctccgagg tcctgcccca
     7201 gatgctgatc aaggcgggac tcgaacccgt catgtggaag acgggacatt cgctcatcaa
     7261 acagaagatg aaagacctcc acgcgccgct tgcgggagag atgagcgggc acatgttctt
     7321 cgggggcgac tggttcggct tcgacgacgc cctcttcggt gccgcccgcc tgctgcacta
     7381 cgtcgcgcgc gaagggggac cgctgtcgcg gctgctcgcc gacctcccgc gcaccgtgaa
     7441 cacgcccgag cttcgcgtgg attgcccgga tgaacgcaag ttcgacgtgg tggagcgcgc
     7501 ggccaagtac ttcggcgcca agtatccggt gtcgacgctc gacggtgtgc ggatgacgtt
     7561 ccccaccggg tgggggctga tccgcgcctc caacacccag cccgttctgg tcatgcgttt
     7621 tgaggcgacg tcggccaccg atctcgagcg gtatcgcgcc gaagtcgagg ggtggctcgc
     7681 gcaaaacgcc ggaggcccgg ggtgacccgg cggcgctggg cgctgctggg cgccgcggcc
     7741 cttctggtcg tggtgctcgt cgggggccgt tggttcgcca tgcagaccgc cgagcacgcc
     7801 tgggccgcca cgattccgaa cggggccgcg tacctccgga ccctcgcgct ggcccgcacg
     7861 gtccaatgga tcgtcgccct cgccgcggtc ctgtggggca tggggcattg ctacggggtt
     7921 taccgtgcca tcgggtcggt gcagatgccc cggcggctcg gcgacatcga gatcgtcgag
     7981 gccgttccgc accgcttgct catcgtcgtg acgctggtcg gcggcgtggt ctatggcggt
     8041 gccttggcct gggccatcgg tgacgtctgg cgaccggcgc tcctcgcgag cgcgccgccg
     8101 cacttcggcc tcacggattc ggtcctccat caggacctcg gctactacgt gggggagctg
     8161 ccctgggcgc gcacccggca ggggatgctg ctcatcgcct cgttgaccgc gtcgttcctc
     8221 atcgccgttc tctatgtcgc catcggatcg attcgcatgc acggcgggcg catcgcggcc
     8281 agcccgcacg cgcgcgcgca cctgggactc acgctcgccg cgctggcggt ggcgttcgtg
     8341 tggggtgccc atctcgatcc ctatgaaacg atcaccggcc tcgtcggtcc cgtaacccac
     8401 gacgcgatcg cgatccgcgg ccctggtgcc agcgccgtca ccgtggtcgc gctgatcacc
     8461 gcgtttgtga gcgcggtgtg gggatggtgg gatcgcccgg gcgtggtcgg ggcgacctgg
     8521 ctcgcactgc tcgtcaccat gctggccgcc tacgtcatcc tgcctcccgc tactcacacc
     8581 atcgggaccc cgtcgccggc ggaccgcgcg ctgcaggacg acaaggggac actcgaagga
     8641 gtcgccttcg gggcggctcc ggaatcctgg aatctcccct cgctgtcgcc cgcggcactc
     8701 gtggcctcga tccccgtgtg ggatccgggg cggatcagga tccgcgctct gcgcgacagc
     8761 gccgtggggc agggactgtc ggtgtacaac atgacgctcg agccctcggg acgctggttg
     8821 ctcgggctcg cgcccgacgg caccaatctg ctccgcgtct cgccgccgcc gacgtgggag
     8881 caggtgcacc aaagcgtgtg ggcacacgcc ccggggcccc tcgaagcgcg agagaccgac
     8941 tcgaccctta cgttcgcccg tgcgccgggg gggccgcgtg cgacgtggtt cggtggcgga
     9001 ttcctcgact acgcggtggt ggactccggc agcatgaccg gcatcccgct gacgggtacg
     9061 tggcggcgcc tggctctggc ctggaccctg caagccccgc agctcgcaca ggtcgcctcg
     9121 cggggccgga cgttggtctg gcgccgggat gccgtggagc gcttcgaggc actggcgccg
     9181 ttcgcacgct tcgagatgcc cgcgcccgtg ctcgtgggcg attccctttg gtggatctcc
     9241 tggggatcgg tcgaagccga cgcgttcccc ctggtcgaca ccgtgatgat cgacgggcgc
     9301 accatccggt atcaccggtc cggtctcgtc ggcgcgctca acgccgtcag cggctcgacg
     9361 cggctctggc tcgcccccca aaccgactcg ctcacggccg cctgggcgcg catcgtggcc
     9421 ccactcgtcg caccggccga ctcgatgccc ccggcattgc ggcgagtcct gccgtacccg
     9481 gctgatgcct ttgaaatcca ggctaggatt ctcgcccgca ccattccgct gccggactcg
     9541 acctaccgct ggcttccgct cgtggccgaa caccccttca cccttgcggc gccaggtccc
     9601 gactcggcga ctgacgtgtg gtacgcgcag gccttcgtcg attctacgag gcagagcacg
     9661 ttcggcggca tcctggccgg cacgatgacg gagaaaggcc ctcgcttgtt cttctggacc
     9721 ccgaagccct cgaccattcc cacgccgctg tcgagtgatc gcgagcgacg ctcgggcgtc
     9781 gagcgggtct ggcctacgga cgttggcgtc atgacggtgc aggcgcgctt cctcgagccc
     9841 gagggcagtc ccgtgcccgc gaagctcgac agcgtctacg tgtcgctggg gccggatgtc
     9901 gcggcggatg tgtccgcccc ggcggccctc aagaccctgc tgcgcgggcc cgacctcgga
     9961 gaggggacac cgacggcgag cctgcgccgc gcccgcgccc tccttgcccg gatggattcc
    10021 gcgctggcag cagggcacgt ggcccgtttc ggggtgctgt acgacagcct gcgagccttg
    10081 ctgcgcggca cttcgcgagg cgttgccccc tccaccggcc cccgctaggt tgcgccgccg
    10141 tgaccggaac cggggtgcgc gtgaaggacg tgatcgtggt ggcgctgggt ggcaatgccg
    10201 tggcgccgca gggcgagcgg ccgaccatcg ccaaccagtt ccggcacacc cgccagagtc
    10261 ttgcgcccat cgtggagctg gcccgcgcgg gatggggcat cgcgctggtg cacggcaatg
    10321 gcccgcaggt cggtgacgag ctggcgcgca acgagttggc cgccgatcaa ctggagccgc
    10381 tgccgttggg cgtgctggtg gcggccaccg cggggtggat gggttacatg atccagcagt
    10441 ccctgcagaa cgctctgcgt cgcgccggtg tggaacggca ggtcgtgacc atggtgaccc
    10501 agaccatctg cgacgcctcg gaccccgacc tgcgccggcc gaccaagccg atcggccacg
    10561 ccctcgatgc ggctacgcgt cagcgtctcg aagcgcgagg tcaggcggta cgcgaggacc
    10621 gtcccgggcg cgcacggcgg gtcgtgccga gccccaaacc gataggggtg gtggagcgga
    10681 agatgatcgc ggacctcgtg agccgcggcc acatcgtggt cgcgtgcggc ggcgggggac
    10741 cgccggtgta cgaggatccg aagctcggga tcgagggact ggatgcggtc gtggacaagg
    10801 accgcgtggc ggcggtcctc ggagccgagc tgaaggccaa ggccctcctg attctgacgg
    10861 acgtcgacgc ggtgtacgca catttcggca cggcgcacca ggaacggctg ggcaagctct
    10921 ccctccgcga cgcggagcgc ttgctggccg gcgacgagct ggggtcgggg agcatgcgcc
    10981 ccaaggtgga agcggcggcg gacttcgtgc gggccggcgg caagggcgct cgggcggtcg
    11041 tcgccgagtt gtcgaaggga ttggaagcgt tggcgggaca atcaggcacg actatcacga
    11101 gggagaacgg gtgaatctgc acgaatatca ggcccgggac atcctgcgga agcacggtat
    11161 cccgatgccc gaaggagcgg tggcgaggac gccggcggaa gtaaaggcga tcgcgcaaaa
    11221 actcggtggc acggtggtca tcaaggccca ggtgcatgcc ggcggccgcg gcaaggcggg
    11281 cggcgtcaag ctcgcgaaga ctccggccga ggcggaacta ctggcctcga agatcctcgg
    11341 catgcagatc aaggggctca cggtcgagaa agtgctggtg gtgcccgcgg cggacatcgt
    11401 gtccgagagc tacgtcggct tcatcgtcga ccgcgcgacc cagcggcccg tgctgatggt
    11461 gagcgccgcc ggcggggtgg acattgagga ggtcgccgcc aagacaccgg agaaaatcca
    11521 ccggcttgcc gtcgatcccc ggtacgggct gctcccccat caggctcagg gactcgcgtt
    11581 ccatctctac caggacgtgg cgcaggtccg gcaggcggcg gatctgatg
//
LOCUS       DASUBP010000161        19587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1190045, whole genome shotgun sequence.
ACCESSION   DASUBP010000161 DASUBP010000000
VERSION     DASUBP010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 19587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19587
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190045"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(213..3491)
                     /locus_tag="VFU75_05925"
     CDS             complement(213..3491)
                     /locus_tag="VFU75_05925"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466408.1"
                     /translation="MKRIIALSFAVVSLTATRAAGPDSLSGGLVWRNIGPLRAGRVSA
                     VSGAVGEAGVFYAGFPTGGLWKTSNAGVTWDPIFDDITSTSSIGAVQVAPSDTNVIYV
                     GTGDMPTGGNINEGDGVYKSTDAGKTWQHIGLEDSRQIPSILVDPKDPNLVLLAAQGN
                     RYAKGGSRGVYRSTDGGKTWTKTLDTGDSVGVERLAWANDAPSTVLAATDLHYVDVNR
                     AFGGFRGGAPNGPTGTMLFKSTDQGLTWHELTGGGLPRLFGRMSIAVAQHTNAQRMYL
                     IGNFGLYRSDDGGANWRQMDAGDRRVGNGQGGYNCGVYVSSSNPDVVITINTSSYISR
                     DGGNTFTGFKGAPGGDDPQQLWIDPTDGSRMLLGMDQGATVTLDGGRTWSPWYNQSTE
                     QVYHISADNSFPYWVYASQQDAGAIRVRSRGNYGEIGPLDWSPTGGWEWGTDVVDPLN
                     NNILYASGAGIQKISFPTEQIRNVSPSVDPESHLRANSDNPLVFAPWNPRELLAGFQY
                     LMASKDGGMHWTKISPDLGYPKGVTPPPDTAQPKPGSPRGGSIQSLSPSTVARGVIWV
                     GTNNGLIKVTRNEGATWSDASIPNLPDSNRTAVQMVEASHFNAGEAYAALDAHNLGDY
                     QPQLYRTRDYGKTWTKIVTGLPTGQIAGSFTHLIREDTKKDGLLFAGTESGMYVSFDD
                     GDHWQSLQLNLPTTSYRDGFIKGNDLVVGTYGRGIWILDDISPLRQMTGSEPAEAVRL
                     FQPGDAIRIRRNVNQDTPMPPEVPHSDNAPDGVLITYYLASAPSGIITLDVLDASGAV
                     VRHMSSAPIPPLKEAATPPNPSFWLATPEPMPAKAGLNRINWDIRYDDPPAFNHSWEI
                     NANPGKTPASPQGPLALPGVYTLKLTVDGRSYTQKVTVRDDPRSPATALALREMHDLH
                     MQILAGLSTSWNGYQQANAVRTSLEADTGSSVAEVATAARTLLARIDTVAGSLGGPSG
                     FFFRRSTSPNFVGVNGALVRQLNQLELGDGAPTPGDLADYASSCSDLSKSVTAWRTIS
                     DKDLVAFNAVLTRNSLKPVAGGPAALTAPTCAAAKGGAAAKKARANAVEQDEDRDPDE
                     PPSE"
     gene            complement(3636..5594)
                     /locus_tag="VFU75_05930"
     CDS             complement(3636..5594)
                     /locus_tag="VFU75_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466409.1"
                     /translation="MLSGSFIHRVMLAAGFSFVAVAANAQAPSPDPRVGLKAGIYNAQ
                     EALWNMRLVSATKRPDGFVVDSAPGDFNYIDSDLAFDGKYVIQGNFHGVLIWDVSQPA
                     HPALVTAMACPGFQNDVSVYGHLLFTSVESTDSHKDCGGAHIADTVSADRVRGVRIFD
                     ISDIKHPRLLTNVQTCRGSHTHTLVTSPADKDNLYVYVSGSSFVRSPNELAGCSGMTP
                     EKDPNSELFRIEVIQVPLAHPEQAKVVSKPDILAGLTQAGTHPEAATDLARQQARVDS
                     VRAAGGFIAKVEGRDVIIGGRFLKMQLDSIVKARGGTGDPTAADTATFRTNMQSIVDK
                     IVGADNTQGPRPGPVQCHDITVYPAIGLAGGACGGYGLLLDIKDPAHPRRIAAASDSN
                     FAFWHSATFSNDGSMVLFTDEWGGGLAPRCRATDDLKWGADALFRIKDSTLAFQSYFK
                     MPAAQTEQENCVAHNGSLVPVPGRTIMVQGWYQGGISVFDWTDPAHPKEIAYFDRGPM
                     DSTKLVNAGSWSAYWYNGMIVSSEIGRGLDILELLPNDLLTQNEIDAAKLVRVAQENV
                     QDQQRIVWPNSPVVAQAYLDQLARSGMTSAQVTETRSALAKAEKATGAARKTALTKLA
                     SRLNSSAAASGDPAKVQALASVIGALAK"
     gene            complement(5621..6205)
                     /locus_tag="VFU75_05935"
     CDS             complement(5621..6205)
                     /locus_tag="VFU75_05935"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEU5466410.1"
                     /translation="MTTRRSFFSLALLAGCLGTAPLRGQGAPAPAGPSDADVAFMSGM
                     IGHHAQAVLMAGWAESHGASPTVQTYCRKVVVSQRDEIGLMKRWLQDHHEPLPDSSLM
                     YGRSMPGMGPMLMPGMLTAAQLAQLDSARGPDWDRLFLEDMIRHHQGAIEMVRQLFNT
                     PGGGKGIVFQFATDVSADQTAEITRMRQMLITLP"
     gene            6376..7047
                     /locus_tag="VFU75_05940"
     CDS             6376..7047
                     /locus_tag="VFU75_05940"
                     /inference="COORDINATES: protein motif:HMM:NF015658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEU5466411.1"
                     /translation="MNTSSYGRLAGALALAAVAACGANRASPNVDPPQGEAAAIARAR
                     ADSIRKPYVKADIDFMSGMIYHHAQAIVMASWADSNQAGPEVKVLCGRIINAQRDEIR
                     ILQGWLQDRNQPIPEPRATGMPMMANGRMQNMLMPGMLSPEQMAALHAAKGADFDRLF
                     LQDMIQHHKGAVQMVKDLFASEGAAQDDMVFKLASGINVDQTTEIARMEKMLVVDDIL
                     SGNSQ"
     gene            7101..9089
                     /locus_tag="VFU75_05945"
     CDS             7101..9089
                     /locus_tag="VFU75_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466412.1"
                     /translation="MRVTHHRISPRLTATAVVLGLVAACSHPVPYGVPVPATDMSRVH
                     PNPDPREGLKAGLWNAGQAAWNLRVVSHTHSSDSVFLNSTNSDLAFTGNYVIQGSYNG
                     YQVWDISNPAAPILHTKEACPASQSDVSVYKNLLFVSGEGNGGRLDCGSEGIKDTVSQ
                     ERLRGLRIFDISDITTPKYIANVQTCRGSHTHTVLADPKDPDNVYVYISGSAPARSPR
                     ELAGCSNAQPSEDSTTSLFRIEVIKVPLAHPEQAAIVSSPRIFHDLTAPPTHGMSAAD
                     KDALDKAKAQGKYVVTVFGNPFVLPDRMIKPMLDSIAKTHGGTAASTADSTALRGAIQ
                     GMVDKMVGRRQQKEGSGPPPGPTQCHDITVYAQIGYAGGACEGYGLLLNIQDPAHPYR
                     VAAVDDSNFSYWHSATFNNDGTKVLFSDEWGGGGGPKCRAKDPKEWGADAIFRISNNQ
                     LTFQSYYKMPAIQTENENCVAHNGSLIPIPGRDVMVQSWYQGGVSVFDWTDAAHPQEI
                     AFFDYGPIDSTKLVGGGTWSAYWYNGVIVSSEIARGLDIFALQPSGFISQNEIDAAKS
                     VHFDQWNTQGQRKFVWPASFALSGAYVDQLERSGGLSAARIAAVREGLKKAEGSSGAS
                     RQGMLSLIATQLEGDVAGSSDAAKVQTLIQSVKDLAGK"
     gene            9158..10411
                     /locus_tag="VFU75_05950"
     CDS             9158..10411
                     /locus_tag="VFU75_05950"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5466413.1"
                     /translation="MTDPYASLRIPDFRRFIGFLATQTLATMMQTVAVGWQIYIVTRD
                     PLALGLVGLAEALPFIAFALPAGHLADRVDRRRLSLAGLSVLVACSATLFLVTRMGLV
                     RAGTAWIIYAVIFVSGIARSYLQPTRTALSADLVPRELYANSITWRSSVWQSAAVIGP
                     ALGGIAVAIGGPSFAYGAAAILLITAWIALFRVGPHPHPHGTVPAANAREFLLGVRFL
                     LTQPVLLGAQLLDLFSVLFAGAEALLPIFATDILHVGATGLGILRAAPAAGSVLMSVY
                     QAHRPLVRRMGRVMLVSVTLYGLCISAFGASRSFALSVTLLLFSGVFDNISVVLRSTL
                     LQTLTPRHLLGRVAAVNAIFIGSSNEIGGFESGVAARLLGAVPAVVLGGFVPILVVAS
                     IAVGIPQLRRLDQIADHAPPVSSTS"
     gene            complement(10493..10867)
                     /locus_tag="VFU75_05955"
     CDS             complement(10493..10867)
                     /locus_tag="VFU75_05955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466414.1"
                     /translation="MSLVRQTSVPAPAAPTPSFVKRFDTPERSVGFGDGRLDIISIGR
                     RQVGKGSFAPGWKWTPTPGVKSGGFTGVVLSGRAKVQTKGGESADLTPGDFFMVTPED
                     ECWVVGVRPCEILYLDGIDALG"
     gene            complement(10890..12326)
                     /locus_tag="VFU75_05960"
     CDS             complement(10890..12326)
                     /locus_tag="VFU75_05960"
                     /inference="COORDINATES: protein motif:HMM:NF017809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HEU5466415.1"
                     /translation="MTPLSSSPSLDPAVCARAVGARDPRFDGLFFVGIITTGIYCRPV
                     CPSRAASQKNRRFFASAASAELAGYRPCLRCRPELAPGLAVIDSVPRLAQVAVRRIAA
                     GALNGRGVPELARDLGVSARHLRRALEREVGASPVELAQTHRLLLAKQLLADTALPVT
                     RVAYAAGFQSLRRFNTVFRERYRMSPTTIRRRIRTTQDAPEPVDYDGFGASAVRLVLA
                     YRAPLAWDVLLDQLRQDALPGVESVAGMRYTRAVRVGKHTGLVAVEPLPDHPQVRVTL
                     SATLLPVLMEVLARLRQVFDLDAEPDVVDDWLARTDLAPLVARHRGLRIPGALDGFDA
                     ALRLVLEGSGAGGRVAQDLGEPIASGDESLNRLAPSADRVADAGAPRLVALGLSPKRA
                     ETLAALARAVADGTLRLEPGREVAPVRDALLAIPGVQEPLAAGILLRALHWPDAFPIA
                     GSRELLARAEQWRPWRGYAALHLRLAAS"
     gene            complement(12491..14824)
                     /gene="pbpC"
                     /locus_tag="VFU75_05965"
     CDS             complement(12491..14824)
                     /gene="pbpC"
                     /locus_tag="VFU75_05965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HEU5466416.1"
                     /translation="MRRARWGVLAVIAALVLFWAMPRVVPHAPLRTRVPTSTAVYDAG
                     GRLLRLTLAPDGQYRAWVPLERISPLLIDATLLQEDRHFRWHPGVDPAALVRAVWHSY
                     VTRDRREGGSTLTMQLARILYGIQSSSLSGKVDQIAHALWLELCYTKREILEAYLNVA
                     PYGGNIQGASAASLIYFDKDPSRLTLAEALTLAVLPQNPVRRGPAAGPLAQATLRIAR
                     DDLYSRWRRHHAAAPGEAEALRLPARIGSPADLPFGAPHFVNTVLAQDAGSSEIRTTL
                     DRRLQNLLERQVRAYVAGQARLGVHNAAAMIVDSRSMSIRALIGSADFFSSAIEGQVN
                     GTQAKRSPGSTLKPFIYGLGIDQGVIHPMTVLKDAPSAFGAYSPENFDGRFVGPLTAQ
                     DALIRSRNIPAVYVASQLSKPDLYDFLSIAGVSGLQSKQHYGLALVLGGGELTMEELV
                     SLYGALSNEGVVRPVRYRADQPEGRGVPVLSPEAAWVTLDMLKANPRPDAPAAAIPAR
                     LPVYWKTGTSYGFRDAWTVGIFGPYVLAVWVGNFDGEGNPAFVGVQMAAPLFFRVVDA
                     VEALEPSLGEPPRTFPSNLTRVDVCAASGDLPNAECPRTVPTWFIPGKSPIRVSTLHQ
                     ALLIDDRTGLRDCPPYTAGAAHRVVYEMWSSDMARLFQEAGLPRRAPPPFDPRCETHV
                     AGNSAGIPPQITSPLRGVTYSLRARRLGTETITLRATASGGVHAVYWFVGSSFIGTSA
                     PGATVGWHPDVAGSFVVRAVDDQGQADSRMVEVAIVP"
     gene            complement(14821..19572)
                     /locus_tag="VFU75_05970"
     CDS             complement(14821..19572)
                     /locus_tag="VFU75_05970"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HEU5466417.1"
                     /translation="MGPDVLAASAPLTLTPIPAEREHTSTVAFKYEADPGRFVYVRVN
                     RGLRSFGGYVLGETWDHTALVPPFPQEVKILGSGSLLSLGGERRISIYSRDVPGLLVE
                     VGRVLPEQLQHLVTQTSLVFGNTEFTNPNFDVGNLTARQSDTIRLPSLGPGKAHYQPY
                     DLGRYLSNGGTPRGLFLVSVQGYDSVAKRTTGPTDRRLILLTDLGLLAKQNGDGSRDL
                     FVQSLRSGQPVAGATVNVVGENGLTVLSAETDGDGHAHFPTLKPFERERTPVLYSVQR
                     GEDLSFLPMDKGDRELDLSRFDIGGVANAIQADKLTAFLFSDRGVYRPGDAFHVGIIV
                     KPADWLTRLEGIPLEIVVNDARGLEVKRQRIRLSAQAFEEFTYTTAETSPTGSWTVSL
                     YIVKDGRAGALLGTVPVLVREFQPDRLAMTAHLSTESQNGWVTPDTLRARISLRNLFG
                     TPAVGRRVSTTIQLTPGFPSLRGFEDYHFLDPQKAKETYTEDLPDVTTDAKGEAEIPL
                     GLERFTRATYRLRVITEGYEPEGGRSVTAEVGAVVSNLPFIVGSKPDGDLQYLHKDSE
                     RKVSLVAVGPDGKALSAAGLHLARIRISYVSVLARQFDGTYKYQSVKKEIPLGDSVVT
                     IPVGTLTVPLVTTEPGTFALAVKDSAGTELSRVEYNVAGAANLARSLERNAELQVSLD
                     KPDYAPGETVELQIQAPYTGSGLITIERDHVYSWRWFHTTTTSSVQHIPIPAGLEGNG
                     YVSVAFTRDANSDEIFTSPLSYGVAPFSVSLERRKQPITLDAPSMVKPGDVVHVHYQT
                     DHPSRIAVFAVDEGILQVASYHTPDPLGFFFQKRALDVRTSQILDLILPEFQQLMDAS
                     APGGDQGAAIGRNLNPFKRKRDKPVAFWSGVRDADAGGGDFTFTAPDYYNGTLRVMAV
                     AVNEDAVGAAETKALVRGDFVLSPNVPLMVAPGDTFDVSVGVANNIARSGKGAPIHVA
                     VQVPTSLQMIGPPSTTIPVDAMGEGAVVFHLRATDKLGNADLTFTASLGDNSAHLTQS
                     LSLRPAVPYRTALIAGTVKQGRASVAVDRDLYPELRAVRGAISVVPLGLARGLAEYLD
                     KYPYGCTEQLVSEGVPAMVLARRPEFGITTAEAATSFGKVIDMLRSRQNEEGAYGLWA
                     GNAHVDQFVSVYAQHFLLDARERGFAVPPEVIANGWRYLIHLASTEGNSLYDERIRAY
                     AIYLLTRSGMVTTGFATALDRRVRENYPKQWTQDLTAAFLASTYQLLKQEGLAGNIIG
                     AMRFNGDRVPAYAEYDDNLTHDSMLLYLLSRHFPSRLPSLQAQAIQGIVGPIQAGAFN
                     SLSSATAILGLDAYVTAVGPQAAGKFTILEKMTDGSQLPATLSTGTFPEAPITVRTRR
                     VTFVSEAAEPAFYLLNQSGFDRTIPAKPLSQQLEVLREYTDKNGNAVSSVGLGDEIQV
                     HLKFRAVGKNSGASVALVDLLPGGFEVVEDGGRAQQPPQVVLNRPVDSTSAEAEGDGG
                     EEGDAGSPELAWTPPFGEAVGSWSPEYVEEREDRVVVYGFAEQNIQEFVYTIKATAAG
                     SFAVPPAFGEGMYDRAVKAWSQAGRMVVKPR"
BASE COUNT         3258 a         6603 c         6500 g         3226 t
ORIGIN      
        1 tgcggctcac gagcggcgac atgacgtcac agatcacgct gaactgtgag aacgtcctca
       61 atacggtgta ccgggacaac ctctcggtga tcaaggactt tgtgccccag ccaggacgtg
      121 gattccggct cgcgtacgac atcctgtact gaggaccgga aagacaagaa ccccgcctcg
      181 acgatgaggc ggggttcttg ttttcagttc ccttactcgc tcggcggttc gtccggatcg
      241 cggtcttcat cctgctcgac cgcgttcgcc cgggccttct tcgcggccgc gccacccttg
      301 gcggcggcac acgtcggagc cgtgagcgcc gccggaccgc ccgccaccgg cttgaggctg
      361 ttcctggtga gcacggcgtt gaaggcgacc aagtccttgt cgctgatcgt gcgccacgcg
      421 gttaccgact tgctgagatc cgagcaactc gacgcgtagt ccgcgaggtc gcccggcgtc
      481 ggcgcaccgt cacccagctc cagctgattg agctggcgga ccagcgcacc gttgacgcca
      541 acgaaattcg gcgacgtgga gcgccggaag aagaagcccg agggaccgcc cagagaaccg
      601 gccacggtat cgatccgcgc cagcaacgtc ctggccgccg tggcaacctc cgccacgctc
      661 gagccggtat cggcctccag gctggtgcgg acggcgttcg cctgctggta gccgttccac
      721 gacgtcgaca gccccgccag gatctgcatg tgcagatcat gcatctcacg cagcgcgagc
      781 gcggtggcgg gggaacgcgg atcatcccgc accgtgactt tctgcgtgta gctcctgccg
      841 tccaccgtga gcttcagcgt gtagacgccc ggcagcgcga gcggaccttg tggcgaggcc
      901 ggggtcttgc cgggattcgc gttgatctcc cagctgtgat tgaaggccgg cggatcgtcg
      961 taccggatat cccagttgat ccgattcaac ccggccttgg ccggcatcgg ctccggcgtc
     1021 gcgagccaga agctcgggtt gggaggggtc gccgcttcct tcaacggcgg aatcggcgcg
     1081 ctggacatgt gacgcaccac cgcgccgctc gcatccagga cgtcgagcgt gatgatgccc
     1141 gagggagcgg aggccaggta gtacgtgatc agcacgccgt cgggtgcgtt gtccgaatgc
     1201 ggaacttccg gcggcatcgg cgtgtcctgg ttcacgttcc gccggatgcg gatcgcgtcc
     1261 ccgggctgga agagcctcac cgcctccgcc ggttccgagc cggtcatctg ccggaggggg
     1321 gagatgtcgt ccaggatcca gatgccgcgg ccgtaggtcc cgaccaccag atcgttcccc
     1381 ttgatgaacc cgtcccggta cgaggtcgtg ggaaggttca gctgcaggga ctgccagtga
     1441 tcaccgtcgt cgaacgagac gtacatcccg ctctcggtgc cggcgaacag gagcccgtcc
     1501 ttcttggtgt cttcgcggat caggtgcgtg aatgacccgg cgatctgccc ggtgggcagg
     1561 ccggtcacga tcttggtcca cgtcttcccg taatcccggg tgcggtacag ctggggctga
     1621 tagtctccca ggttgtgcgc gtcgagcgcc gcgtacgctt ccccggcgtt gaaatgcgac
     1681 gcctcgacca tctgcacggc ggtccgattg gaatccggga gattcgggat cgaggcatca
     1741 ctccacgtgg cgccctcgtt ccgtgtgacc ttgatcagcc cgttgttcgt accgacccag
     1801 atcaccccgc gcgcgaccgt cgacggcgag agggattgga tgctcccgcc gcgcggggaa
     1861 cccggcttgg gctgagcggt gtcgggaggc ggggtgactc ccttggggta gcccagatcg
     1921 ggactgatct tcgtccagtg catcccgccg tctttactcg ccatgaggta ctggaacccg
     1981 gccaagagct cgcgcggatt ccagggcgcg aacaccagcg gattgtcgga gttggcccgc
     2041 aggtgcgatt ccggatcgac ggacgggctc acgttgcgga tctgctcggt cgggaatgaa
     2101 atcttctgga tgccggcccc ggacgcgtag aggatgttgt tgttgagcgg atcgacgacg
     2161 tcggtgcccc actcccagcc gcccgtcgga ctccaatcca ggggtccgat ctcgccgtag
     2221 ttgccccggc tccggacccg aatggccccg gcgtcctgct gcgaggcgta gacccagtac
     2281 gggaacgaat tgtccgccga gatgtggtag acctgctcgg tggactggtt gtaccaggga
     2341 ctccaggtcc gcccgccatc gagcgtcacc gtcgcgccct ggtccatgcc gaggagcatg
     2401 cgcgagccgt cggtggggtc gatccagagc tgctggggat cgtcaccgcc cggggcgccc
     2461 ttgaagccgg tgaacgtatt gccgccgtcg cgcgagatgt agctcgacgt attgatcgtg
     2521 atcaccacgt ccgggttcga ggagctgacg tagacgccgc agttgtagcc gccctgcccg
     2581 tttccgaccc ggcggtcccc cgcgtccatc tgccgccagt tggcgccgcc gtcgtcggag
     2641 cggtagagac cgaaattccc gatgaggtac atccgctgcg cattggtgtg ctgagccaca
     2701 gcgatggaca tccggccgaa gagccggggc agtccgcccc ccgtcaactc gtgccaggtg
     2761 aggccctggt cggtcgactt gaagagcatc gtgccggtgg ggccgttggg agcaccgccc
     2821 cggaatccgc cgaacgcccg gttcacatcg acgtaatgca ggtccgtggc cgccagcacg
     2881 gtgctcggcg catcattcgc ccaagccagc cgctcgacgc cgaccgaatc gccggtgtcc
     2941 agggtcttgg tccaggtctt cccgccgtcg gtgctccggt acacgccgcg cgagccgccc
     3001 ttggcgtagc ggtttccctg tgcggcgagg agaacgaggt tggggtcttt gggatcgacg
     3061 agaatcgagg ggatctggcg ggagtcttcg aggccgatgt gctgccaggt cttgccggca
     3121 tcggtcgact tgtagacgcc gtccccttca ttgatgttgc cgccggtcgg catgtcgccg
     3181 gtgccgacgt agatcacgtt ggtgtccgac ggcgcgacct gcacggcgcc gatggaggac
     3241 gtgctcgtga tgtcgtcgaa gatgggatcc cacgtgacac ccgcgttcga tgtcttccac
     3301 aggcctccgg tcgggaaccc ggcgtagaac acgcctgcct cgccgaccgc gccggagacc
     3361 gcggagacgc gtccggcacg gagtggaccg atgttgcgcc acaccaggcc gcccgacaac
     3421 gagtcggggc ccgcggcgcg tgtagcggtg agtgaaacga cggcgaagga gagggcgatg
     3481 atgcgcttca tgtcgcagag cctctggggt tcggggaaca acgggaatat gcgtcgtccg
     3541 tccttcatac ggagcctccc ctttccctgt tgcacgaaac ggaacggccc cacccggtgg
     3601 agtggggccg tcgggagacc gcctcctgat gggcgctact tggccagggc cccgatcacc
     3661 gaagccaggg cctgcacctt ggccgggtcg cccgaggctg ccgccgagct gttgagccgg
     3721 gacgccagct tggtcagcgc cgtcttccgg gccgccccgg tggccttttc cgccttggcc
     3781 agcgcggaac gggtctcagt cacctgcgcg gaagtcatcc cggaccgggc cagctggtcc
     3841 aggtaggcct gggccacgac cgggctgttc ggccacacga tccgctgctg atcctgcacg
     3901 ttctcctggg ccacacgcac gagcttcgcc gcgtcgatct cgttctgggt gagcaggtcg
     3961 ttcggcaaga gctccaggat gtcgagcccc cgcccgatct ccgagctgac gatcatgccg
     4021 ttataccaat aggcggacca ggagccggcg ttcaccagct tggtcgagtc catgggaccg
     4081 cggtcgaagt aggcgatctc cttggggtgg gcgggatcgg tccagtcgaa caccgaaatg
     4141 ccgccctggt accagccctg gaccatgatg gtgcggcccg gaacaggcac cagcgacccg
     4201 ttgtgggcga cacagttctc ctgctcggtc tgggcggcgg gcatcttgaa gtagctctgg
     4261 aaggccagcg tcgagtcctt gatccggaag agggcatccg cgccccactt caggtcatcg
     4321 gtggcgcggc agcgcggcgc caggcccccg ccccactcgt cggtgaacag caccatcgat
     4381 ccgtcgttcg agaaggtcgc cgaatgccag aaggcgaagt tcgagtcgga cgcggcggcg
     4441 atccgccgcg ggtgcgccgg atccttgatg tccagcagga ggccgtagcc accgcaggcc
     4501 ccgccggcca gaccgatcgc cggatacacc gtaatgtcgt ggcactgcac cgggcccgga
     4561 cgggggccct gggtattgtc cgcgccgacg atcttgtcga cgatggattg catgttggtg
     4621 cggaaggtcg ccgtatcggc agccgtcggg tcaccggtgc cgccgcgggc tttcacgatg
     4681 ctgtcgagct gcatcttgag gaaccgcccg ccgatgatca cgtcccggcc ttcgaccttg
     4741 gcaatgaacc cgccggccgc gcgcaccgaa tccacgcgcg cctgctgccg ggcgaggtcc
     4801 gtcgccgcct cggggtgggt gccggcctgc gtgagccccg cgagaatgtc gggcttgctg
     4861 acgaccttcg cctgctccgg atgcgccagg ggcacctgga tcacttcgat gcggaagagc
     4921 tcgctgttcg gatccttctc cggtgtcatc cccgagcaac ccgccagctc attgggtgag
     4981 cggacgaagg acgagcccga gacatagacg tagaggttgt ccttgtccgc ggggctggtg
     5041 accagcgtgt gggtgtggga accccggcac gtctgcacgt tggtgagcag cctgggatgc
     5101 ttgatgtccg agatgtcgaa gatgcggacg ccgcggaccc ggtcggcgct caccgtatcg
     5161 gcgatgtggg cgccgccgca gtccttgtgg ctgtccgtcg actcgaccga cgtgaagagc
     5221 agatgcccgt acaccgagac gtcgttctgg aacccggggc acgccatcgc cgtcacgagc
     5281 gccggatggg ccggctgact cacatcccag atcaggacgc cgtggaaatt cccctggatg
     5341 acgtatttgc cgtcgaaggc caggtcggaa tcgatgtagt tgaaatcacc gggcgcggaa
     5401 tccaccacga acccgtcggg gcgcttggtc gccgacacga ggcgcatgtt ccagagcgcc
     5461 tcctgcgcat tgtagatccc ggccttcagc ccgacccgcg gatccgggga cggcgcctgg
     5521 gcattcgcgg cgaccgccac gaacgaaaac ccggcggcca gcatcacgcg gtgaatgaaa
     5581 gaaccagaga gcacgggaaa ctccggagtg gaaggtgacg tcacggaagc gtgatcaaca
     5641 tctgccgcat ccgcgtgatt tccgcggtct gatcggccga aacatcggtg gcgaactgga
     5701 agacgatccc cttgccgccg cccggcgtat tgaagagttg ccggaccatt tcgatggcgc
     5761 cctgatgatg acggatcatg tcctcgagga agaggcggtc ccagtcgggc ccgcgggcac
     5821 tgtcgagctg cgcgagctgc gccgcggtga gcatgccggg catcagcatc ggccccatcc
     5881 ccggcatgga ccgcccgtac atcaggctcg agtccgggag gggttcgtgg tggtcctgga
     5941 gccagcgctt catcaggccg atctcatccc gctgcgaaac cacgaccttg cggcaatacg
     6001 tctgaaccgt gggcgaggcc ccatggctct cggcccatcc cgccatgagc accgcctggg
     6061 cgtgatgccc gatcatgccg gacatgaagg caacatcggc atctgacggc cccgcaggcg
     6121 ctggggctcc ctggccccgg agtggggccg tgcccaggca accggccagc agtgcgagag
     6181 agaagaatga gcggcgggtg gtcatggtag cccgatagga ttctacagcg tcgccctgga
     6241 aacggaaccg tccccatcca tcccgccggg gtgaccttcc gccccagcgt cccaggacgc
     6301 atcttgtcca ccttcaccgg accgggagcc gcccccggcc cgaccgggtt ccatgttgcg
     6361 aggagcagta ccgtgatgaa cacgtcgtca tatggccgcc tggccggcgc actcgctctg
     6421 gccgcggtgg cggcctgcgg tgccaatcgt gccagtccga acgtagaccc cccacaaggc
     6481 gaggccgccg ccatcgcccg cgcgcgcgcc gacagcatcc gcaaaccgta cgtcaaagcc
     6541 gacatcgact tcatgtccgg catgatctac caccacgcgc aggccatcgt gatggccagc
     6601 tgggcggatt cgaatcaggc gggacccgag gtcaaggtcc tctgcgggcg catcatcaac
     6661 gcgcaaaggg acgagatccg gatcctgcag ggatggttgc aggaccggaa tcagccgatc
     6721 ccggagccgc gcgcgaccgg catgccgatg atggcgaacg gccggatgca gaatatgctg
     6781 atgcccggaa tgctctcccc cgagcagatg gccgcgctcc acgccgcgaa gggcgcggac
     6841 ttcgaccgcc tgttcctgca ggacatgatc cagcatcaca agggcgcggt gcagatggtg
     6901 aaggatttgt tcgcgtccga aggggccgca caggacgata tggtcttcaa gctggcctcc
     6961 ggcatcaacg tggaccagac caccgagatc gcccggatgg agaagatgct ggtcgtcgac
     7021 gatatcttga gcggaaactc gcagtgaacc gaggtttgtt gccgtcaatt ggatccacac
     7081 cgttcccaag gatcaacacc atgcgcgtca cgcatcaccg catttcaccc cgtctgaccg
     7141 ctacggccgt cgtcctgggc ctcgtggcgg cctgctcgca cccggtgcca tacggcgtcc
     7201 cggtgccggc aaccgacatg agccgggtgc atccgaaccc ggacccgcgt gaggggctga
     7261 aggccggcct ctggaatgcg ggccaagcgg cctggaatct ccgggtggtc tcgcacacac
     7321 actcctccga ctcggtgttc ctgaacagca ccaactccga cctggccttc acaggcaact
     7381 acgtgatcca gggcagctac aacggttacc aggtctggga tatctcgaat ccggcggcgc
     7441 cgattctcca caccaaggaa gcgtgccccg catcccagag tgacgtctcc gtctacaaga
     7501 acctgctgtt cgtctcgggt gaggggaacg gcggccggct cgactgcggc tccgaaggca
     7561 tcaaggacac cgtgagccag gagcgcctgc ggggcctccg catcttcgac atctctgaca
     7621 tcacgacgcc caagtacatc gcgaacgtcc agacctgccg cggctcgcac acccacacgg
     7681 tgctcgccga tccgaaagat ccggacaacg tctacgtcta catctccggc tccgccccgg
     7741 cccgctcgcc ccgcgagctc gcgggatgca gcaacgctca gccgagcgaa gattcgacca
     7801 cttcgctctt ccggatcgaa gtgatcaagg tcccgctcgc ccatcccgag caggccgcga
     7861 tcgtcagctc cccccggatc ttccacgact tgaccgcgcc cccgacgcac ggcatgtccg
     7921 ccgccgacaa ggacgcgctg gacaaggcca aggcgcaagg caagtacgtc gtcacggtct
     7981 tcgggaaccc gttcgtgctt ccagaccgca tgatcaagcc gatgctggac agcatcgcca
     8041 agacccacgg cgggaccgcg gcgtcgacgg cggacagcac cgccctgcgg ggtgccatcc
     8101 aggggatggt cgacaagatg gtcgggcgcc ggcagcagaa ggagggctcg ggcccgccgc
     8161 cgggaccgac ccagtgccat gacatcacgg tctatgccca gatcgggtac gcgggcggcg
     8221 cctgcgaagg ctacggattg ctcctcaaca tccaggaccc cgcgcatccg taccgcgtcg
     8281 cggccgtgga cgactcgaac ttctcctact ggcactcggc cacgttcaac aacgacggga
     8341 ccaaggtgct cttctcggat gagtggggtg gcggcggcgg gccgaagtgc cgcgccaagg
     8401 atccgaagga gtggggcgcc gacgcgatct tcaggatcag caacaaccag ctcacgttcc
     8461 agagctacta caagatgccg gccatccaga ccgagaacga gaactgcgtg gcccacaacg
     8521 gctcgctgat cccgatcccc ggacgggacg tgatggtgca gtcgtggtat cagggtggcg
     8581 tgtcggtgtt cgactggacc gatgccgcgc atccgcagga gatcgcgttc ttcgactacg
     8641 gccccatcga ctccaccaag ctcgtgggtg gcggtacgtg gtcggcctac tggtacaacg
     8701 gcgtcatcgt cagctccgag attgcgcgcg gcttggacat cttcgcgctg cagccctccg
     8761 gcttcatctc gcagaacgag atcgacgccg cgaagagcgt ccacttcgac cagtggaata
     8821 ctcagggaca gcggaaattc gtctggcccg cgtcgttcgc gctctccggc gcgtacgtcg
     8881 atcagctgga gcgttcgggt gggctctccg ccgcccggat tgcggcggtg cgcgaaggcc
     8941 tcaagaaggc tgagggatcg tcgggcgcca gccggcaggg gatgctgagc ctgatcgcca
     9001 cccagctgga gggtgacgtg gccggctcga gcgacgccgc caaggtgcag acgctcattc
     9061 agtcggtgaa ggatctcgcc ggcaagtaag cgtcgagtcc cgtgatgcat gagggcccca
     9121 tcgccgaaag gcggtggggc ccttaacgtt cgcgcccatg acggatccgt acgcgtccct
     9181 gcgcatcccc gacttccggc gcttcatcgg cttcctggcc acccagaccc tggccacgat
     9241 gatgcagacc gtggccgtgg gctggcagat ctacatcgtc acgcgcgacc cgctcgccct
     9301 gggactggtc gggctggccg aggccctgcc cttcatcgcc ttcgccctgc ccgccggcca
     9361 tctcgcggac cgggtagacc gtcgccggct ctccctggcc gggctgtccg tcctggtggc
     9421 gtgcagtgcg acgctctttc tggtcacgcg gatgggattg gtgcgcgccg gcacggcctg
     9481 gatcatctat gcggtgatct tcgtgtccgg gatcgcccgc agttacctgc agcccacgcg
     9541 caccgccctg tccgcggacc tcgtcccccg ggagctctac gcgaactcca tcacctggcg
     9601 ctcgtccgtc tggcagtccg ccgccgtcat cggccccgcc ctcggcggca tcgccgtggc
     9661 gatcggcggt ccctcgttcg cctacggggc cgcggcgatc ctcttgatca cggcctggat
     9721 cgcgctcttc cgcgtcggcc cccaccccca tccgcacggc accgtgcccg cggcgaacgc
     9781 ccgcgagttc ctcctgggcg tgcgctttct gctcacccag ccggtgctcc tgggcgccca
     9841 gctcctcgac ttgttctcgg tcctgttcgc cggcgccgag gccctgctgc cgatcttcgc
     9901 gaccgacatt cttcatgtgg gtgcgaccgg actgggcatt ctccgcgccg cccccgccgc
     9961 gggatcggtc ctcatgtccg tctatcaggc gcaccggcct ctcgtgcgcc ggatgggacg
    10021 ggtcatgctc gtctccgtca ccctctacgg cctctgcatc tcggcgttcg gcgcgtcccg
    10081 gagtttcgcc ctctccgtga cactgctcct gttctcgggc gtcttcgaca acatcagcgt
    10141 ggtactccga tccacgttgc tgcagacgct cacgccccga cacctgctcg gccgggtggc
    10201 ggcggtgaac gcgatattca tcgggtcgtc gaatgagatc gggggattcg agtcaggcgt
    10261 ggcggcccgg ctcctgggcg ccgtgcccgc ggtcgtgctg gggggattcg tacctattct
    10321 ggtggtcgcc tcgatcgccg tcggcattcc ccagctgcga cggctcgatc agatcgcgga
    10381 tcacgctcct ccggtcagtt cgacgtcctg agtcaggggt caggggggag ggggtcgggg
    10441 cgtcaaccga cccctgaccc ccgccgcccg ccgcccgccg ccccatcgct gctcaaccga
    10501 gggcgtcgat gccatcgagg tagagaatct cgcagggacg cacgcccacc acccagcatt
    10561 cgtcctcagg ggtcaccatg aagaagtcgc caggggtgag gtcggcgctc tcgccgccct
    10621 tcgtctgcac cttggcccgg ccggagagca cgacaccggt gaagccgccc gacttcacgc
    10681 cgggtgtggg ggtccatttc caccccggag cgaacgaccc ttttcccacc tgccggcggc
    10741 cgatgctgat gatgtccagc cgtccatcac cgaatccgac cgaacgttca ggagtgtcga
    10801 agcgcttcac gaaggagggt gtgggagcgg cgggagcggg gacactggtc tgacgaacaa
    10861 gactcatggg gctctccttg agagggcgtt catgatgctg ccaggcggag gtgcaacgcc
    10921 gcgtaccccc gccagggccg ccattgttcc gcgcgtgcga ggagctcgcg cgatccggcg
    10981 atcggaaacg catcgggcca gtgcagcgcg cggagcagga tcccggcggc caggggctcc
    11041 tgcactccgg gaatggccag gagcgcgtcg cggaccggtg ccacttcgcg tcccggctcg
    11101 agacgcaggg tgccatccgc caccgcgcgg gccagcgccg ccagcgtttc cgcccgtttg
    11161 ggcgagagac cgagggccac gagccgggga gcaccggcgt ccgccacgcg gtcggcggag
    11221 ggtgccaacc ggttgagcga ttcgtctccg ctggcgatcg gctcacccag gtcctgcgcc
    11281 actctccccc cggccccact gccctcgagc acgaggcgca acgcggcgtc gaatccgtcc
    11341 agcgcgcccg ggatgcggag gccccgatgc cgtgccacga gcggtgccag gtccgttcgg
    11401 gcgagccaat catccacgac atcgggctcc gcatccaggt cgaacacctg ccgcaaccgg
    11461 gcgaggacct ccatcagcac cgggagcaac gtggctgaca gggtgacccg cacctgcggg
    11521 tggtcgggca gcggctccac cgccaccaga cccgtgtgct tgcccacgcg cacggcccgg
    11581 gtgtaccgca tgcccgcgac cgactccacc ccgggcaagg catcctggcg cagctgatcg
    11641 agcagaacat cccacgccag cggcgcgcga tacgccagga ccagacgcac ggctgacgca
    11701 ccgaacccgt cgtagtccac cggctccggc gcgtcctgcg tcgtacgaat ccgccggcga
    11761 atggtcgtcg gagacatccg gtaccgttcg cggaacaccg tgttgaaccg gcgcaagctc
    11821 tggaacccgg ccgcgtacgc cacgcgggtc accggcaacg ccgtgtccgc caggagctgt
    11881 ttggcgagga ggagacggtg cgtctgcgcg agctcgacgg gtgaggcgcc gacttcgcgc
    11941 tccaatgcac ggcggaggtg acgcgcgctg acgcccaggt cacgggcgag ctccggcaca
    12001 ccgcggccat tcagggcgcc cgcggcgatg cggcggaccg cgacctgcgc cagccgcggc
    12061 acggagtcga tcaccgccag ccccggtgcc agctcgggcc ggcaccggag acagggccgg
    12121 taccccgcga gctcggcgga cgcggctgac gcgaagaacc gccggttctt ctggctggcg
    12181 gcgcgggagg gacaaacggg ccggcagtag atgcccgtgg tgatgatgcc cacgaagaag
    12241 agcccatcga accgcgggtc ccgggcgccg acggcccggg cgcagacggc cggatcgagc
    12301 gagggggagc tggagagcgg agtcatgggt tcaatggtac ccggcagccc gcctcaggct
    12361 ggccggtttc ggacacggaa ttgtcattga accgtagcct agaggtccgc ctggcgaacc
    12421 tcaatccggt cttcggcgga ccgcccggaa cccgcgacgg ctcgctgtgc atgtgcgccg
    12481 actgggcggg tcagggaacg atcgcgacct ccaccatccg gctatccgcc tggccctggt
    12541 catcgacggc ccgcacgacg aatgaccccg cgacgtccgg atgccatccc acggtggctc
    12601 cgggcgcgga agtcccgatg aagctggagc ccacgaacca gtacaccgca tgcaccccac
    12661 cgctggccgt cgcacgcagg gtgatggtct cggtaccgag acggcgggcc cggagcgagt
    12721 acgtcacgcc acgaaggggc gacgtgattt gcggcgggat ccccgcggag ttgccggcga
    12781 cgtgcgtttc gcagcgcgga tcgaatggcg gcggagcgcg ccgcggcagt ccggcttcct
    12841 gaaagagccg cgccatgtcc gagctccaca tctcgtagac gacgcgatgc gcggcgcccg
    12901 cggtatacgg cgggcaatcc cgcaggcccg tgcgatcgtc gatcagcaag gcctggtgca
    12961 gtgtgctgac gcgaatggga gacttgcccg ggatgaacca ggtcggcacg gtccggggac
    13021 actcggcgtt cggcaggtcc ccggaagcgg cgcatacatc gacgcgcgtc agattggagg
    13081 gaaaggtgcg cggtggctcg ccgagtgacg gctcgagcgc ttcgacggca tcgaccaccc
    13141 ggaaaaagag cggagccgcc atctgtacgc ccacgaacgc cggattccct tcaccatcga
    13201 agttgccgac ccacaccgcc aggacgtagg gtccgaagat gcccaccgtc cacgcgtccc
    13261 ggaatccgta cgaggtcccg gtcttccagt acaccgggag gcgtgccgga atggcggccg
    13321 ccggcgcatc agggcgtggg ttggccttga gcatgtcgag cgtcacccac gcggcttcag
    13381 ggctgagcac cggtactccc ctgccctccg gttgatcggc ccggtaacga accggtcgga
    13441 ccactccctc gttgctcaag gctccgtaca gcgagaccag ctcttccatg gtgagttcgc
    13501 ccccacccag caccagcgcg agtccgtagt gctgcttcga ctgcaaaccc gacacgccgg
    13561 cgatcgacag gaagtcgtag agatcgggct tggagagctg ggaggcgacg tatacggcgg
    13621 ggatgtttcg gctacgaatc agcgcgtcct gagcggtcag cggccccacg aagcgtccat
    13681 cgaagttctc cggactgtac gcgccgaacg cggagggagc gtccttgagc accgtcatgg
    13741 gatggatcac tccctgatcg atccccaacc cgtagatgaa cggcttcagc gtagatcccg
    13801 gcgaccgctt ggcctgggtc ccgttcacct ggccttcgat tgccgagctg aagaagtcgg
    13861 cggaaccgat cagggcccgg atggacatgg aacgggagtc gacgatcatc gccgcggcgt
    13921 tgtgcacacc caggcgcgcc tgtccggcga cgtacgcgcg cacctgtcgc tccaggagat
    13981 tctgcaagcg ccggtccaac gtggtcctga tctcggagct cccggcatcc tgggccagga
    14041 cggtgttcac gaagtgcggc gcgccaaagg ggagatccgc gggcgatccg atccgggccg
    14101 gcagccgcag cgcttcggct tctcccggtg ccgctgcatg gtgccgtcgc caccggctgt
    14161 agaggtcgtc acgcgcgatg cggagggtcg cctgcgccag tggacctgcc gcgggtcccc
    14221 gtcgcaccgg gttctggggc aggaccgcca gggtcagcgc ctcagccaac gtgagccgcg
    14281 acgggtcttt gtcgaaataa atgagactcg ccgcggacgc gccttggatg ttccccccat
    14341 acggagcgac gttgagatac gcctccagaa tctcccgttt ggtgtagcac agctcgagcc
    14401 agagtgcgtg cgcgatctgg tcgaccttgc cactcagcga ggaggactgg atcccataaa
    14461 ggatccgggc gagctgcatg gtgagcgtgg atccgccttc gcgccggtcc cgggtgacgt
    14521 acgagtgcca caccgcgcga accagtgcgg ccgggtcgac gcccggatgc cagcggaagt
    14581 gccggtcctc ctgcagcaat gtggcgtcga tcaagagcgg cgagatccgc tcgagtggca
    14641 cccaggcccg gtactgacca tccggtgcca gggtcaagcg cagcagacgc ccaccggcgt
    14701 cgtacaccgc cgtcgatgtc ggcacgcgtg tccggagcgg agcgtggggg acgactcgcg
    14761 gcatggccca gaacaggacg agcgccgcga tgacggccag aactccccag cgcgctcgtc
    14821 tcaccgcggc ttcaccacca tccgtcccgc ctgcgaccag gcctttaccg cccggtcgta
    14881 catgccttcg ccgaatgccg ggggcaccgc aaatgacccc gcggccgtcg ccttgatcgt
    14941 gtagacgaac tcctggatgt tctgctccgc gaagccatac acgacgacgc ggtcctcacg
    15001 ctcctccacg tactccggcg accagctccc cacggcttct ccgaagggcg gggtccaggc
    15061 gagctccggg gacccggcgt cgccctcttc tcccccatca ccttcagcct cggccgacgt
    15121 cgaatccacc ggtcggttca agacgacctg tggaggttgc tgggctctcc cgccatcctc
    15181 gacgacctcg aatccgccgg gcaggaggtc gacgagggcc accgacgcac ccgagttctt
    15241 gcccaccgca cggaacttga ggtgcacctg aatctcgtcc ccgagaccaa cgctcgagac
    15301 cgcattgcca ttcttgtccg tgtactcgcg caggacttcg agctgctgcg acaggggctt
    15361 tgccggaatg gtccggtcga accccgactg gttcaggaga tagaaggcgg gttcagcagc
    15421 ttcactcaca aacgtgactc ttctggtacg caccgtaatc ggcgcctccg ggaacgtccc
    15481 ggtcgagagc gtcgcgggaa gctgcgaccc gtcggtcatc ttctccagaa tggtgaactt
    15541 gccggccgcc tgcgggccga cggcggtgac gtatgcatcg aggccgagaa tcgcggtcgc
    15601 cgaggacagc gagttgaacg cccctgcctg aatcggcccg acgatgccct gaatcgcctg
    15661 cgcctgaagg gacggaagcc gtgacgggaa atgccgcgac aggagataca gcaacatgga
    15721 atcgtgcgtc aggttgtcgt cgtactccgc gtatgccggc acccgatccc cgttgaaccg
    15781 catggcgccg atgatgtttc ccgccagccc ctcctgcttc aggagctgat aggtcgacgc
    15841 gagaaaggcc gcggtgaggt cttgagtcca ttgcttggga tagttctccc gcacccgacg
    15901 atccaaagcg gttgcgaacc cggtggtcac catccccgaa cgcgtcagca ggtagatcgc
    15961 gtacgcccgg atccgctcgt cgtacaggct gttcccttcc gtactcgcca ggtgaatgag
    16021 gtaccgccag ccattggcga tgacctcggg gggaacggca aagccgcgct cccgcgcatc
    16081 gagcaggaaa tgctgggcgt agacggacac gaattgatca acgtgcgcgt tgccggccca
    16141 cagtccgtag gccccctcct cattctgccg ggagcgcagc atgtcgatga ctttcccgaa
    16201 gctggtcgca gcctcggcgg tggtgattcc gaactccggc cggcgggcga gcaccatggc
    16261 gggcacccct tcgctcacca actgctcggt gcacccatag gggtacttgt cgagatactc
    16321 ggcgagcccg cgggcgaggc cgaggggcac gacggaaatg gcaccccgaa cggcccgcag
    16381 ctccggatac aagtcgcggt cgacggcaac agacgcgcgt ccctgtttca ccgttcccgc
    16441 gatcagcgcg gtgcggtagg gtaccgcggg cctgagactc agactctgcg tcaggtgcgc
    16501 ggaattgtcc ccgaggctcg cggtaaaggt caggtccgcg ttccccagct tgtcggtggc
    16561 ccgaaggtgg aagaccaccg cgccctcccc catggcgtcc accggaatgg tggtcgaggg
    16621 aggcccgatc atctgaagag acgtcggtac ctgtaccgcg acatggatcg gggcaccctt
    16681 cccggatctc gcgatgttgt tcgcaacccc gacgctcacg tcgaacgtgt ccccgggtgc
    16741 caccatgagc ggcacgttcg gagacagcac gaagtcaccc cggacgagcg ccttggtctc
    16801 cgcggccccg accgcgtctt cattcaccgc caccgccatc acgcgcaacg tcccgttgta
    16861 gtagtcgggc gcggtgaacg tgaagtcccc tccaccggcg tcggcgtccc ggacgcccga
    16921 ccagaaggcg acgggcttgt cccgcttccg cttgaaggga ttgaggttcc gcccgatcgc
    16981 cgccccctga tcaccccccg gcgccgacgc gtccatcagc tgctgaaatt cgggaaggat
    17041 caggtcgagg atctggctgg tgcgcacatc cagggcgcgc ttctggaaga aaaagccgag
    17101 ggggtcgggt gtgtgatagc tcgccacctg gaggatgccc tcatccaccg cgaataccgc
    17161 gatccgggac gggtggtcgg tctggtagtg cacgtgcacg acgtcccccg gctttaccat
    17221 ggacggggca tccagggtta tcggctgctt ccggcgttcc agactcactg agaagggcgc
    17281 gacgccgtaa ctcagcggac tggtgaagat ctcgtcggag ttggcatccc gcgtgaaggc
    17341 gacggacacg taaccgttgc cttcgagacc ggcgggtatg gggatgtgct gcaccgagct
    17401 ggtggtcgtg gtgtggaacc accgccagga gtacacgtga tcgcgctcga tcgtgatcaa
    17461 accggagccc gtgtagggag cctgaatctg cagctcgacc gtctccccgg gcgcgtagtc
    17521 aggcttgtcg agactcacct ggagctcggc attgcgctcg agggagcgag cgaggttggc
    17581 ggccccggcc acgttgtatt ccacccgcga cagctccgta cccgccgagt ccttcactgc
    17641 cagcgcgaat gttccgggct cggtggtcac cagtggaacg gtcagggtgc ccaccggaat
    17701 cgtcactacc gagtcgccca gcgggatctc cttcttcacc gactggtact tgtaggtccc
    17761 gtcgaattgg cgcgcgagga cggacacgta gctgatccga atcctggcga ggtgaagccc
    17821 agcggcgctg agggccttcc cgtcgggacc gacggcgacg agcgagactt ttcgctccga
    17881 gtccttgtgc aggtactgca ggtctccgtc gggcttggac ccgacgatga acggcaggtt
    17941 ggatacgacc gccccgacct ccgcggtcac gctccggccc ccctccggct catagccttc
    18001 cgtgatcacc cgcagacggt acgtcgcccg cgtgaagcgc tcgagtccga gcgggatctc
    18061 ggcctcaccc ttcgcgtcgg tggtgacgtc cggcaaatcc tcggtatagg tctccttggc
    18121 tttctgggga tccaggaagt ggtagtcctc gaagcctctc agcgacggga aaccgggcgt
    18181 gagttgaatg gtcgtcgaga cccgccgccc cacggccggc gtaccgaaca ggttccggag
    18241 ggagatccgc gcccgcagtg tgtccggtgt gacccagcca ttctgcgatt ccgttgagag
    18301 gtgcgccgtc atcgccaacc ggtccggctg gaattcgcgc accagcaccg gtaccgtgcc
    18361 caggagcgcc ccggcccgtc cgtccttcac gatgtagagg gacaccgtcc acgatccggt
    18421 gggcgacgtt tcggccgtcg tgtacgtgaa ctcctcgaac gcctgggccg agagcctgat
    18481 gcgctggcgc ttgacctcaa gaccgcgggc atcatttacg acgatctcga gggggatccc
    18541 ctcgagccgg gtcagccaat ccgcgggctt cacgatgatg ccgacgtgga aggcgtcacc
    18601 gggccggtac accccacggt cggagaagag gaacgcggtg agcttgtccg cctggatggc
    18661 gttggccacg ccgccgatgt cgaaccggga caggtcgagc tcgcggtccc ccttgtccat
    18721 cggcaggaac gagaggtcct cccctcgctg caccgagtac agaaccggtg tccgctcgcg
    18781 ctcgaaaggc ttcagcgtgg ggaagtgcgc gtgcccgtcc ccgtcagtct ccgcggagag
    18841 aacggtgagg ccgttttccc cgacgacgtt gaccgtcgcc ccggcgaccg gctgtccgga
    18901 gcggagtgat tggacgaaga gatcgcggct gccgtcgcca ttctgcttgg cgaggagtcc
    18961 caggtcggtg agcagaatga ggcgccggtc agtggggccg gtcgtccgtt tggccaccga
    19021 gtcgtaccct tgcacggaga ccaggaagag tcctcgcggc gtcccgccgt tggacaggta
    19081 acggccaagg tcgtagggct ggtagtgcgc ctttccggga ccgagcgacg ggaggcggat
    19141 cgtatcggac tggcgagccg tgaggttgcc cacatcgaag ttcgggttgg tgaactccgt
    19201 attcccaaac accaggctgg tctgcgtcac gaggtgctgc agctgctcgg gcaggactcg
    19261 cccgacctcg acgagcaggc cggggacgtc acggctatag atggatatcc gccgctcccc
    19321 acccaggctc aagagcgatc ccgagccgag gatcttcact tcctgcggga agggcggcac
    19381 cagggccgtg tgatcccagg tttccccgag gacgtagccg ccgaaggagc gcagcccgcg
    19441 gttgacacgg acatagacga agcggcccgg atcagcctcg tacttgaacg cgacggtgga
    19501 ggtgtgttcg cgttcggcgg gaatcggcgt cagggtcagg ggagccgatg ccgccaggac
    19561 atcggggccc acctcggcag gccccca
//
LOCUS       DASUBP010000162         5040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_604219, whole genome shotgun sequence.
ACCESSION   DASUBP010000162 DASUBP010000000
VERSION     DASUBP010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5040
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_604219"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..765)
                     /locus_tag="VFU75_05975"
     CDS             complement(<1..765)
                     /locus_tag="VFU75_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIM-barrel domain-containing protein"
                     /protein_id="HEU5466418.1"
                     /translation="MRRLAFALASMPATLTAQVRVPPISFQSFSRDGRVLPASAIRVR
                     AISRGITEISIVAGRQSDWEFRFQEHAPVFGFGERYNTLDQTGQVIVNRSSDTPGPKG
                     TATYVPIPFFMSLSGYGLWLDTYAEASFDLGKTTPGEFVVRLRDSRLRMLVFSGPGFP
                     KILERFTGVVGRTRLPPYWAFGPWKSRNWHPDMAAVYEDVDRYRSLGIPGDVLVLDSP
                     WATNYNTFVMNRLQFTDPEAMIKHVHDQGFKLCLWLT"
     gene            complement(767..3316)
                     /locus_tag="VFU75_05980"
     CDS             complement(767..3316)
                     /locus_tag="VFU75_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466419.1"
                     /translation="MILAACSDSTGPNNAGGGQSIKVGQTVSVNLTPADSFQDFLFRG
                     STDSVYVVEVGPTTGSAYVSVLDSIHLFLNSFTTAGTSTDSAHLWTQTFAGPASDPWV
                     IRLRSAAPGQTTALTIKVLQVATGPEHISSTITPDSVTNGESLDGYDDFDRFTFPGTA
                     GQLVELFVQGTATGAPTQAVCFVLDTFDTHFLALSVSGAQDSVLEAPINGRVSLPTTR
                     TYRVEASSSRNGCRDGFGPDFGYLGPYRMLVYRIDTLPEQVSDTVAVGDTVKGEAVDR
                     VGDIDVFALHGTAGQLVDLQFASPSSGSKRQLQVFVSGAATSPSTFSTGGDTSLDEHA
                     TGRFVLPGTGQVTLRVDGVRDGRGGDAGPYVLYAYPVNPNPEHLPIAITLGDTVTGEA
                     IDHVGDLDQFTFTATAGQLFNGFLQASGAAPSAQITLQVLGPDSTAASGVANSTGADV
                     TLLDQVTGRFQAPATGTYTVRVSGANQDAGPYRFAILPVSRAPETASSSLAFGDSVSN
                     EAIDFPGDLDEFHTVITDSSGANIVLTPDSGAIHLPLQAFLYDSVTGVNIAAVTAFDA
                     GFPAQTGTMVVPPCHCILRVVGGYDSTSTFRGGYTLKFYKFGFGSETVGDTVAIGDTI
                     VGEAINPPGDRDDFVFHATKGQHVNVALEGESVASPGQFQLIFQSVVYSPTAPDSLNA
                     HRTNRIDIPSTGWYAVSVSGSAFTNPLTAPGAYKLALEPVPVGPEHAAATLAVGDSVT
                     NESIDYPGDWDEFTLTGTPGTKVAVSFTIQGAGGNPHLEVFDSTADSVVALAVGQPGV
                     QVAGPATFPASGQLVIRVFEVRGPFGNCTDTYCNGIFGYTGGYKITTAVAP"
     gene            complement(3427..4155)
                     /locus_tag="VFU75_05985"
     CDS             complement(3427..4155)
                     /locus_tag="VFU75_05985"
                     /inference="COORDINATES: protein motif:HMM:NF025716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HEU5466420.1"
                     /translation="MRRASFLFWLDLVLLLTFVLLQEPETTGLWGHEWISIGLAALLL
                     LHLLVNWRWIVGALRRYATNSRRHRINTWLNGLLYVTMVFTIFSGVIVSRFVLPSLGM
                     VSSEVKLWKQLHSLVADGTLGILGLHVAINWDWIVNVFRRWRRGATHPETHGGRSLAL
                     RLGLEGVRRTTVRAGKLAGATAVICALSYLPVAALARERDPAGPETRWAHPDFGDIPG
                     EVTAQLLVVAGAAVVGRYVFKIRL"
     gene            complement(4180..4713)
                     /locus_tag="VFU75_05990"
     CDS             complement(4180..4713)
                     /locus_tag="VFU75_05990"
                     /inference="COORDINATES: protein motif:HMM:NF017496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HEU5466421.1"
                     /translation="MTADELLNIHVPNKRVELVRGVPMVREPAGYIHGRVTMNLTARL
                     VLFLERQPPGPAGQLIAAATGFTLAKAPDTVRSPDIAYFRPGKVPDPETPGFSDAAPD
                     LVVDVLSPEDRTREIQERAGQWLVAGTQVVWVVDPRRQTTHIYRSDGTEEFVGPEGSL
                     RGEDILPGFTCPLEAIM"
     gene            complement(4758..>5040)
                     /locus_tag="VFU75_05995"
     CDS             complement(4758..>5040)
                     /locus_tag="VFU75_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466422.1"
                     /translation="QAGVTPTAGKASLGLLPVDCTGAAVAGALVYTSPSAQAYTNGAG
                     DFFVFNAAPGSTNVGASFQSHIFHTHGVIAWADAVTVTVIAPGPLSPPE"
BASE COUNT          975 a         1584 c         1669 g          812 t
ORIGIN      
        1 cgtgagccag agacagagct tgaagccctg gtcgtggacg tgcttgatca tcgcctcagg
       61 atccgtgaac tggaggcgat tcatcacgaa ggtgttgtag ttggtggccc agggactgtc
      121 gagtaccagc acgtcgccgg gaatgccgag ggagcggtag cggtcgacgt cctcgtagac
      181 cgcggccatg tccggatgcc agttccggct cttccagggt ccgaaggccc aatagggcgg
      241 aagcctggtc cgcccgacca ctcccgtgaa acgctcgagg atcttcggga aaccgggacc
      301 gctgaacacc agcatgcgaa gtcgcgaatc ccgcaggcgc accacgaatt cacccggcgt
      361 tgtcttcccc agatcgaagg acgcttcggc gtacgtatcg agccagagac catacccgga
      421 gagcgacatg aagaacggaa tcggcacgta cgtcgcggtg cccttcggtc cgggcgtgtc
      481 actcgaccgg ttgacgatca cctgccccgt ctgatcgagc gtgttgtagc gctcgccgaa
      541 tccgaagacc ggggcatgtt cctggaaccg gaactcccaa tcggactgtc tgccggcgac
      601 gatcgagatc tcggtgatcc cgcgggagat agcgcgcacg cgaatggccg aagcggggag
      661 cacccggcca tcgcgtgaga acgattgaaa ggaaatgggc ggcacccgca cctgcgcggt
      721 gagtgtcgca ggcatggacg ccagggcgaa ggcaaggcgt ctcacctcag ggtgcgacag
      781 ctgtggtgat cttgtagcct cccgtgtatc cgaaaatgcc gttgcagtag gtgtcggtgc
      841 agtttccgaa aggaccccgg acctcgaaga cgcgaatcac cagctggccc gatgcgggga
      901 aggtcgccgg tccagcgacc tgcacacctg gctggcccac ggcgagggcg accacgctgt
      961 ctgcggtgct gtcgaagact tcgagatggg gattcccgcc cgctccctgg atcgtgaacg
     1021 acaccgcgac cttcgtcccc ggcgttcccg tcagcgtgaa ctcgtcccaa tccccgggat
     1081 agtcgatcga ttcgttcgtc acggagtcac cgaccgcaag cgtggccgcg gcatgctccg
     1141 gaccgacagg caccggctcg agggccagct tgtaggcgcc gggggccgtg agcggattcg
     1201 tgaacgccga accgctcacc gacaccgcgt accatcccgt cgacgggatg tcgatccggt
     1261 tggtccgatg ggcattgagc gaatcgggtg ccgttggact gtagacgacg ctctgaaaga
     1321 tgagctggaa ctgccccggg gaggccaccg attccccctc gagcgccacg ttcacatgct
     1381 ggcccttcgt ggcgtggaac acgaaatcgt cgcggtcgcc gggaggattg atcgcctccc
     1441 cgacgatcgt atccccgatc gccacagtgt cgccgacggt ctcggacccg aatccgaact
     1501 tgtagaactt cagcgtatac ccgccccgga aggtcgaagt cgagtcatag ccccccacga
     1561 cccgcaaaat gcagtggcag ggtggcacaa ccatggtccc cgtttgcgcc ggaaatcctg
     1621 cgtcgaaggc cgtcaccgcg gcgatgttga cccccgtgac cgaatcgtag aggaaggcct
     1681 ggagcggcag atgaatggcg cccgaatcgg gcgtgagaac gatgttcgcc ccgctcgagt
     1741 cagtgatgac ggtgtggaac tcgtccaggt cacccgggaa atcgatcgct tcgttcgaga
     1801 cactgtcgcc gaaggcgagc gacgacgacg cggtctcagg cgcccggctt accgggagga
     1861 tcgcgaagcg atacgggcca gcgtcctggt tcgcaccgga cactcgcacg gtatacgtcc
     1921 ccgttgcggg cgcctgaaag cgaccggtga cctgatcgag gagcgtaacg tcggcacccg
     1981 tgctgttcgc gacgcccgag gccgccgtcg aatcggggcc aagcacctgc aacgtgatct
     2041 gcgccgacgg cgccgcgccc gaagcctgca ggaacccatt gaagagctgg ccggccgtcg
     2101 cggtaaacgt gaactggtcg aggtcgccca cgtggtcgat ggcctcgccg gtcacggtat
     2161 ccccgagagt gatggctatc ggcagatgct cgggattcgg attcaccgga tacgcgtaga
     2221 ggacgtacgg tccggcgtcc cctcctcgtc cgtcgcgcac accatcgacc cgcaaggtga
     2281 cctgtcccgt acccggcaac acgaaacggc cggtggcgtg ttcatcgagg gaggtgtctc
     2341 ccccagtgct gaaggtcgac ggagaggtcg ccgcgccgct gacgaacacc tgcaactggc
     2401 gtttgctccc gctcgaagga gacgcaaact ggaggtccac caattgaccc gcggtgccgt
     2461 ggagcgcgaa gacgtcgata tcccccacgc gatcgacggc ctcccccttt accgtgtccc
     2521 ccacggcgac ggtatcggag acctgctccg ggagcgtatc gatgcgatag accagcatgc
     2581 gatacggacc gagatatccg aagtcaggcc caaacccgtc tcggcaacca ttgcgtgagc
     2641 tcgaggcctc gacccggtac gtccgggtcg ttgggagtga cacccgcccg ttgatgggag
     2701 cctcgagaac gctgtcctgc gcgccgctca cggagagcgc caggaagtgg gtgtcgaagg
     2761 tgtccaggac gaagcacacc gcctgagtcg gcgcgcccgt cgcggttcct tgcacgaaga
     2821 gctcgaccag ttgaccggcc gtccccggga acgtgaaacg gtcgaagtcg tcatagccgt
     2881 cgagggactc accgttggtc acggaatcgg gcgtgatggt cgagctgatg tgctcgggac
     2941 cggtggcgac ctgaaggacc ttgatggtca acgctgtcgt ttgtcccggc gcggccgatc
     3001 ggagccggat cacccaggga tcggatgccg ggcccgcgaa cgtctgcgtc cagaggtgag
     3061 cggagtccgt ggacgtgccc gcggtcgtga aactgttgag gaacaggtgg atcgagtcga
     3121 ggaccgagac gtaggcgctg ccggtcgtag gtcccacctc gacgacgtac accgaatccg
     3181 tgctgccccg gaagagaaag tcctggaaag agtcggcggg cgtcagattg accgagacgg
     3241 tttggccgac ctttatcgat tgaccaccac ccgcattgtt cggcccggta gaatcggaac
     3301 aggcggcgag gatcacgagc gcgcccaaca agcgcgtgga agacggacgt aggagtctca
     3361 gtgtacggcc ctgtcgacga gaggagaatg ggaagtttga gcgttttcgt gatttgtcaa
     3421 ctgccttcat aaccggatct tgaacacgta ccgtcccacc actgccgcac ccgccaccac
     3481 caagagctga gcggtcacct cacctggaat gtcgccgaaa tccggatgcg cccagcgggt
     3541 ctccggacca gccgggtcgc gctcgcgcgc gagcgccgcg accggcaggt acgaaagcgc
     3601 gcagatgacc gccgtggcgc ctgccagctt gccggcacgg acggtagtgc ggcggacccc
     3661 ttccaggccg agtcgcaggg cgagagaccg gccaccatgt gtttccgggt gcgtggcgcc
     3721 gcgccgccac cgcctgaata cgttcacgat ccaatcccaa ttgatcgcga catgtaggcc
     3781 gaggatgccg agggtgccgt ccgcgacgag gctgtgcagc tgcttccata gcttgacctc
     3841 gctcgacacc atgccgagcg atggcagaac gaagcgcgac acgatgacac ctgaaaagat
     3901 ggtgaagacc atcgtgacgt agagcaggcc gttgagccag gtattgatgc ggtgccggcg
     3961 gctgttggtc gcgtaccggc ggagggcgcc gacgatccag cgccaattca ccaagagatg
     4021 aaggagaaga agagcggcaa ggccgatgct gatccattcg tgcccccaga gcccggtagt
     4081 ttcgggctcc tggaggagca caaaggtcag caggagtacg aggtccaacc agaagagaaa
     4141 gctggctcgg cgcatgaccc aatgtacgca ccacccccgc tacatgatgg cctccagggg
     4201 gcacgtgaat ccgggcagga tgtcctcgcc tcggagtgat ccctccggcc ccacaaactc
     4261 ctcggttcca tcggaccggt agatgtgcgt cgtctggcgc cggggatcca cgacccacac
     4321 cacctgcgtg cccgccacca accattgccc agctcgctcc tgaatctccc gcgtccggtc
     4381 ctcggggctc aggacgtcga ccaccagatc cggggcggca tcagagaatc cgggggtctc
     4441 cgggtcgggc accttccccg gccggaaata ggcgatatcg ggcgaccgga cggtgtcggg
     4501 cgccttggcg agggtgaagc cggtggcggc cgcaatcagc tgaccggccg ggcccggcgg
     4561 ttggcgttcg agaaagagca ccaggcgcgc cgtgaggttc atggtcaccc ggccgtggat
     4621 gtacccggcg ggctcgcgaa ccatcggcac accgcgcacc agctcgacgc gtttgttggg
     4681 aacatggatg ttcaagagct cgtcagcggt catacggaag cttacagccc gtgtatcaac
     4741 aaacagtcaa atggcggcta ctcggggggc gagaggggtc ccggggcgat gaccgtaact
     4801 gtcacggcat ccgcccaagc gatgacgccg tgggtgtgga aaatgtgact ctggaagctc
     4861 gcgccaacat tcgtgctccc cggggcggcg ttgaacacga agaaatcccc ggcgccgtta
     4921 gtgtatgcct gtgccgaggg actggtgtac acaagcgctc cggcaacggc ggcaccggtg
     4981 caatcgaccg gcaggaggcc aagggaggct ttgccggccg tcggcgtcac gccggcttgg
//
LOCUS       DASUBP010000163         2551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_140876, whole genome shotgun sequence.
ACCESSION   DASUBP010000163 DASUBP010000000
VERSION     DASUBP010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140876"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1527
                     /locus_tag="VFU75_06000"
     CDS             <1..1527
                     /locus_tag="VFU75_06000"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF014904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU5466423.1"
                     /translation="LGDYERAVELAAGGKATLILSVESAAHFHPNTPEQVVNACYQAG
                     FRTVHRGVLGDELVAREYLRLFAEPGWGTLIRSTCPVVVEQIRTEHPELVPYLAPVDT
                     PIAAEAKYLKAMYGNDLNVVYAGVCLTEGGSAVDAAITFDDLARLLEARGIKVEDQLP
                     HFERLPEERRRHWSTAGGLPLELLAEQTQASRRFRKVRGLAQLDAMARAVTVDRIDLG
                     FVDILPCDGCLDHPLLGSKEQLFWRRAVVEATEPPRSKLPVLDDEVAQRVNVGATFAV
                     NGRMTAVAPDEVDDVLAEIGSAPNGRPWDCGACGYRSCREFAGAMVKGRTTYKSCPPY
                     LEKQAKAAQQQAAVDGLTGLATFRVLRDRLANEVARTKRSGESFAVLFVDLDHFKQVN
                     DQFGHEAGNEVLRGASRELLAAIRTADFAARYGGDEFVVILVRTGLSGAVGVAEKLRE
                     RVVALGGELGYPKGFIGASIGVAEYRLEGAEHEDVLVAADRALYRAKAGGRNQVATGA
                     "
     gene            1532..2281
                     /locus_tag="VFU75_06005"
     CDS             1532..2281
                     /locus_tag="VFU75_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466424.1"
                     /translation="MTPQKPVRASGAGDAPEQKSSTVSATPAHDPWGVKRAENLITSL
                     TGVLSARVVVTPQGEVSEIHVLTQNDMHPKQVVRNIESALMAQLGLKIDHRVISVAQT
                     ADVRPIEQMQDDAVKNRAKRRVVIFRALDVRPASRAQRVLIRVKLAFEDREADAEEQG
                     TDTIRNRVEAAARAASHCLDELMPDNSIALEGATLVEAFDRKFVLVAVHGLGGREAML
                     MTGTAEVRESPERSAVLAVLDATNRWADARR"
BASE COUNT          428 a          685 c         1002 g          436 t
ORIGIN      
        1 cttggcgact acgaaagggc ggtcgagctc gccgccgggg gcaaggcgac gctcatcctg
       61 agcgtcgaat cagcggccca cttccatccc aacactcctg agcaggtcgt caacgcctgc
      121 tatcaggcgg gtttccgcac cgtccaccgg ggcgttctgg gagacgagct ggtggccagg
      181 gaatacctga ggctgtttgc cgagcctggg tggggcactc tgatccgcag tacttgcccg
      241 gtggtggtcg agcagatcag gaccgagcac ccggagttgg tgccctatct ggcgcctgtg
      301 gatacgccga tcgccgccga agcgaaatac cttaaagcca tgtatggcaa cgacttgaat
      361 gtcgtctacg ccggcgtctg ccttacggaa ggggggtctg cggttgatgc ggccatcacc
      421 ttcgatgacc tggccaggct tctcgaggcc cggggcatta aggtagagga ccaattgccc
      481 cattttgagc gtctgccgga ggaacggcgg cggcattgga gcacggcggg ggggttgccc
      541 ctggagctgt tggcggagca gacccaagcg tcgcgccgat tcaggaaggt gcgcgggctc
      601 gctcagctcg acgcgatggc gcgggcggtg acggtggacc ggatcgacct gggcttcgtg
      661 gacattctgc cctgcgacgg ctgtctcgac cacccgctgc tggggtcgaa ggagcagctc
      721 ttctggcgcc gggcggtagt ggaggcgacg gagccgccgc ggagcaagtt gccggtcctg
      781 gatgacgagg tggcgcagcg ggtgaacgtg ggcgcgacct tcgcggtgaa tggccggatg
      841 acggcggtgg cgcccgacga ggtcgacgat gtcctggcgg agatcggctc ggcgccgaat
      901 ggccggccgt gggattgcgg ggcgtgcggg taccggagtt gccgggagtt cgccggcgcg
      961 atggtgaagg ggcggacgac gtacaagtcg tgcccaccgt atctggagaa gcaggcgaag
     1021 gccgcgcagc agcaggcggc ggtggatggg ttgacgggcc tggcaacgtt ccgggtcttg
     1081 cgagaccggt tggcgaacga agtggcgcgg accaagcggt ccggtgaatc gttcgcggtg
     1141 ctgttcgtgg atctggacca cttcaagcag gtgaacgacc agttcgggca cgaggccggg
     1201 aacgaggtac tgcgcggggc gtcgcgcgag ttgctggccg cgattcgtac ggcggacttc
     1261 gccgcccgtt acggcggcga cgaattcgtg gtgattctgg tgcgtacggg gctctcggga
     1321 gcggtggggg tcgcggagaa attgcgggag cgggtggtgg cgctgggggg agagctgggc
     1381 tatccgaagg gctttatcgg cgccagcatt ggggtggctg agtaccggtt ggagggagct
     1441 gagcacgaag atgtgctggt cgcagccgat cgcgcgctgt accgggcgaa ggcgggcggc
     1501 cggaaccagg tagcaacagg agcgtaatgc cgtgacaccg cagaagccgg tccgcgccag
     1561 tggcgcgggg gatgcgcccg aacagaagag ctcgacggtt tccgcgacgc cggcccacga
     1621 tccgtggggg gtgaagcggg ccgagaatct catcaccagt ctcacggggg tgttgtccgc
     1681 gcgggtggtg gtgacgccgc agggggaagt gagcgagatc cacgtgctca cgcagaacga
     1741 catgcacccc aagcaggtgg tgcggaacat cgagtcggcg ttgatggccc agctcggtct
     1801 caagatcgat caccgggtga tcagcgtggc gcagacggcg gacgtgcgtc cgatcgagca
     1861 gatgcaggac gacgcggtga agaaccgggc caagaggcgg gtggtgattt tccgcgcgct
     1921 ggacgtccgg ccggcgtcgc gggcccagcg ggtgctgatc cgggtgaagc tcgcgttcga
     1981 ggaccgggag gcggacgccg aggagcaggg gacggacacg atccggaacc gggtggaggc
     2041 ggcggcgcgc gccgcgtcgc actgtctgga cgagctgatg cccgacaatt cgatcgcact
     2101 cgagggggcg acccttgtgg aggcgttcga ccggaagttc gtgctggtgg cggtgcatgg
     2161 gctgggtggc cgcgaggcga tgctgatgac cgggacggca gaggtcaggg agagtcccga
     2221 gcgcagcgcg gtgttggccg tgttggatgc gacgaatcgg tgggctgacg cgcggcgatg
     2281 accaggggtc aggtgtcggg tgtcaggggt caggaaagca cctgtagcgt tgagtagacg
     2341 ttgtgttggc gatttttagc gtcgtaagtt gttgttgtac aatatcttat aggcatatcc
     2401 gtcccccatt gcagcgccct cctgggggga ctagcggact atcgattctg gtggtatttc
     2461 gggttccgtg agttctatca aagcgaagaa gtactggggc acgcagcgcg tcgcgcgcat
     2521 ctgtcaggtg tcgcctacga cggtggccaa c
//
LOCUS       DASUBP010000164        18229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_372511, whole genome shotgun sequence.
ACCESSION   DASUBP010000164 DASUBP010000000
VERSION     DASUBP010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 18229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18229
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_372511"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VFU75_06010"
     CDS             <1..657
                     /locus_tag="VFU75_06010"
                     /inference="COORDINATES: protein motif:HMM:NF013355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEU5466425.1"
                     /translation="RGGMLNAVGLANPGLEEVAAHDLPWLLDRVKRARVLVNVAGATV
                     EDYVKVLERLGGIDRVTAFEINASCPNTSAGGLEFGADEASLAHLVRRCRGATTKPLV
                     IKLSPVLPDIAHAGEVARDAGADGLSLVNTMPATLEGRLGNGFGGMSGPALLPVGVLA
                     IRKVRARVALPLIGVGGIRTGADAREYLHAGADLVAIGTAALADPRVPERIVRSLNGG
                     "
     gene            650..1348
                     /gene="pyrF"
                     /locus_tag="VFU75_06015"
     CDS             650..1348
                     /gene="pyrF"
                     /locus_tag="VFU75_06015"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF001273.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEU5466426.1"
                     /translation="MAELVVAFDLPTGKEALALASRLPGLGWAKIGPVLYVREGPALV
                     REFTARGIRVFLDLKWHDIPNTVAGAVEGARELGVSLATVHCLGGAAMLSAAGKAAGA
                     QGTTGAISLVGVTVLTSHDAGGFEGVVGRGVPDLGLEVVRLARLARDAGLRGVVASGH
                     EVGLLREALGAEPWLVVPGIRAPDAPADDQARTVTPGEAVRRGATHLVVGRPITGAKD
                     PLSAFRGVLEAMTT"
     gene            1425..1541
                     /gene="rpmJ"
                     /locus_tag="VFU75_06020"
     CDS             1425..1541
                     /gene="rpmJ"
                     /locus_tag="VFU75_06020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEU5466427.1"
                     /translation="MKVRTSVKPICEHCKVIKRRGVVRVICKRNPKHKQRQG"
     gene            1546..1929
                     /gene="rpsM"
                     /locus_tag="VFU75_06025"
     CDS             1546..1929
                     /gene="rpsM"
                     /locus_tag="VFU75_06025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEU5466428.1"
                     /translation="MARIAGVDLPRDKKIQFALPYIYGVGPSNAKRILAETGVSPDAR
                     VRDLRDAEVARLRQVIERDYKVEGALRTEVAMNIKRLMDIGTYRGGRHRKNLPVRGQR
                     THTNARTKKGPRRAIAGKKKPSLKK"
     gene            1930..2322
                     /gene="rpsK"
                     /locus_tag="VFU75_06030"
     CDS             1930..2322
                     /gene="rpsK"
                     /locus_tag="VFU75_06030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEU5466429.1"
                     /translation="MAQAKAAGAKRAKKVVEAEGVAHITATFNNTLITITDSHGNAIT
                     WGSAGKAGFKGSKKSTPFAATVAAEQAAREALSLGVKRVHVRVQGPGSGRESAIQALA
                     TAGLQIRSIRDVTPIPHNGCRPPKKRRV"
     gene            2326..2961
                     /gene="rpsD"
                     /locus_tag="VFU75_06035"
     CDS             2326..2961
                     /gene="rpsD"
                     /locus_tag="VFU75_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002614801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEU5466430.1"
                     /translation="MARYTGPACRLCRREGTKLFLKGARCLTEKCAIERRAYAPGQHG
                     QGTGRRRKASEYAKQMREKQKVKRIYGLSERQFRNVFERVLREPGVTGEALLIALESR
                     LDNIVFRMGFASSRRQARQLVRHRHIQVNARTVDIPSYRIRPGDEVKVADASRELALV
                     KVAAELAGRGQPVSWIQVDSDKALGKMTERPTRDAIPVAAQEQLIVELYSK"
     gene            2961..4025
                     /locus_tag="VFU75_06040"
     CDS             2961..4025
                     /locus_tag="VFU75_06040"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003513.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEU5466431.1"
                     /translation="MTTTLDLTGLVRPHVVEMTKREDTPNAAEFRLQPLERGYGYTLG
                     NSLRRMLLSSLRGAAVWAFRLDGVVHEHQTIPGVVEDVHQIIQRLKQLTLVLAPDADE
                     AVLHIKREKAGPVYARDIEAHGAVTVVNPDQLLFTVQDDKEMNGELYVNKGRGYVEAE
                     QHPVDRTLSVDVVRIDSIYNPVRRANFTVAETRVGQRTDYDRLTLSVETNGTISPEDA
                     VAYAAALAQEHFRYFVDFGKVPMVAAEAPSAGGGSAAPGRLKELLDRSIDDVGLTVRS
                     VNSLKNSNIRTLGDLVQHAEADLLKVKNVGDKALGEIADLLRREGLNFGMRYEETGTG
                     DVRVLEAGVPPMAQVASGDE"
     gene            4025..4381
                     /gene="rplQ"
                     /locus_tag="VFU75_06045"
     CDS             4025..4381
                     /gene="rplQ"
                     /locus_tag="VFU75_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEU5466432.1"
                     /translation="MRHRAKGRQLSRTSSHKKATLNNMATSLFRHDRIVTTEAKAKEL
                     RPFAEHLITLARRGDLHARRLVERRIADKEVSHRLFAELGKRFAARPGGYTRIVKLGH
                     RVGDGADVARIELLSE"
     gene            complement(4440..4631)
                     /gene="rpmB"
                     /locus_tag="VFU75_06050"
     CDS             complement(4440..4631)
                     /gene="rpmB"
                     /locus_tag="VFU75_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014398134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEU5466433.1"
                     /translation="MARVCDLCGKGPATGHNVSHANNKSNRRWFPNLQRVRAVVNGTT
                     RRIRVCTRCIKSHRVTKAA"
     gene            4646..5698
                     /gene="gmd"
                     /locus_tag="VFU75_06055"
     CDS             4646..5698
                     /gene="gmd"
                     /locus_tag="VFU75_06055"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016509474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEU5466434.1"
                     /translation="MSSTARTPRGSGPEQGRKPNGGSEAGQPRQAQPKTAVITGVTGQ
                     DGSYLAELLLEKGYEVVGVVRRTSHDSYERIGHLLDRVHIVPADLLDQHSLTAVIRDA
                     KPHEVYNLAAQSFVPTSWSQPVLTGEFTALGVTRLLEAVRLAHPEARFYQASSSEMFG
                     KAQETPQKETTSFYPRSPYGVAKVYGHWITVNYRESYGLYAVSGILFNHESPRRGLEF
                     VTRKVTHAVARIKRGRGTEVRLGNLDARRDWGYAGDYVDAMWRMLQQPMPADFVIGTG
                     ETHTVRELCEIAFGHAGLDWQRHVVIDPKFVRPAEVDLLLADPSRAKAELHWAPKTGF
                     ADLIKMMVDADLERLA"
     gene            5695..6660
                     /locus_tag="VFU75_06060"
     CDS             5695..6660
                     /locus_tag="VFU75_06060"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HEU5466435.1"
                     /translation="MKVLVTGADGFVGRWMIRRLLTDGHEVFGALRPAGATHEDGLSD
                     QERESVRWLPLELTDADSVRMVVDLPYDAVIHLAAVASGPEAARDPGYAWTVNAAGTA
                     RIAYVLAEARRKGRADPVFLLVSTGEVYGRGLDGKNGRPRLETDLLAPCSPYASSKVG
                     AETAALEAWRRTGLRVVVARAFAHTGPGQDTRFVVPAFAQRLRYARQIGAPAVKVGNL
                     EPVREFLDVRDVVDAYTRLLAKGVPGEAYNVASGQGIELRQLFDKVAARLGVKAIPET
                     DPELMRPADIPNLVGDGSKLRAATGWAPTFSLDDTLRDTVDAQAH"
     gene            6644..9937
                     /gene="carB"
                     /locus_tag="VFU75_06065"
     CDS             6644..9937
                     /gene="carB"
                     /locus_tag="VFU75_06065"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEU5466436.1"
                     /translation="MPKRTDLKRILLIGSGPIVIGQGAEFDYSGTQAVKALREEGYEV
                     VLVNSNPATIMTDPELADQTYVEPVTPAWVRKVIEKERPDALLPTMGGQTALNVAMAL
                     AQDGTLAEFGVQLIGANARAIKMAEDRAEFAIAMRRIGLETPLGRTVSSREEALAAVA
                     ETGYPAIIRPSFTLGGTGGGVAYNRAEFEELVDTALELSPVHSTLIERSVIGWKEFEL
                     EVMRDGRDNVVIVCSIENIDAMGVHTGDSITVAPAMTLTDREYQKMRDSAIAIIREIG
                     VEAGGCNIQFAVNPADGEMLVIEMNPRVSRSSALASKATGFPIARIGAKLAVGYTLDE
                     LPNDITRTTPASFEPVLDYVVVKVPRFAFEKFPRADFHLTTQMKSVGEAMAIGRTFKE
                     AFQKGLRALETGRTGWSLGATLADDRLTGDSPEDIRVALRVPTPERVFQIKRALLAGL
                     TVPDIAGASGIDPWFLHQFAELIAAERAYAADPKPGAAGIRRMKRLGFSDRQLAALKG
                     ETEAAVRERRWALDIHPVYKTVDTCAGEFPSSTPYLYSCYDHENESEPLGEQGIVILG
                     SGPNRIGQGVEFDYCCVRAGLAFRELGYKTIMINSNPETVSTDFDISDKLYFEPLTFE
                     DVLEIIHWERPKGVVVQLGGQTPLRLTKPLEAAGVHILGTTPDAIDIAEDRERFDALA
                     RRLGVTQPANGMARSVDEAVKVAERIGFPVLIRPSYVLGGRAMEIVYDVGSLRQFFEV
                     AARVAPEHPVLIDRFLEDAFEADVDAISDGKRTVVGGVMQHIEDAGVHSGDSACVLPP
                     YLIGDRQVAEMRKHTKAFAEALGVVGLINVQYAVKDGTVYVLEVNPRASRTIPFVSKA
                     TGVSLAKLAAAVMVGHGLDELGVPDELPLPGVAVKEAVFPFTKLPGVDSVLGPEMRST
                     GEAMGFADSFGMAFAKAQIAADSGLPLDGAIFVTVNDSDKPTVVPIVRRFHELGFRIL
                     ATAGTQAYLRARGVPAESVRKVHEGRPNAIDLLVSGDIQLLINTPLGKFTQQDDYAIR
                     RAALVHRIPYTTTMSAASAACDAIIALRSRTGSVRSLQAWHPKPLVGNQPRQVPKTPA
                     PSPGAAVRA"
     gene            9850..10866
                     /gene="murB"
                     /locus_tag="VFU75_06070"
     CDS             9850..10866
                     /gene="murB"
                     /locus_tag="VFU75_06070"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU5466437.1"
                     /translation="MAPKTPRRKSAASGTKDARPKSRSSGKGVAKAAAAKPVKAPPAP
                     PQPPKAKFRGRLLEQEPLARYTTYRIGGPAKYLLFPADVDDVQRGLEMAQNRNLPWVV
                     LGLGSNVLVKDSGFPGVVIRLGKGLDRFEMKGATAIVGAGMPIPILARRTAEAGFVGV
                     ERFIGIPGTVGGGIYMNAGCHGAEFSEVVTEITVMDAKGKVKQIARKQISFKYRASNL
                     GPVIVLEAKLVLGEESPAKLKELQSRLFRWRKAGTPYDQPCCGSVFKNPSLTRTAGML
                     IDEAGLKGHTVGGAQVSNLHANYFINLGNATAADVQKLMDHVKKTVQKKTGVELEPEV
                     RVIG"
     gene            10866..11453
                     /locus_tag="VFU75_06075"
     CDS             10866..11453
                     /locus_tag="VFU75_06075"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEU5466438.1"
                     /translation="MADYYVHPSSFLDEGAVVGAGTKIWHFCHVMKGAVIGERSSLGQ
                     NVVVMPGTSIGNNVKIQNNVSIYEGVHLEDDVFCGPSCVFTNVLNPRSHVSRKHEYRQ
                     TLVKRGASIGANATIVCGTTLGEFAFIGAGAVVTSDVPAYGLMVGVPARRVGWMCQCG
                     ERLSISAGAAQCAACGAEYAESGGTLRITKPPRPA"
     gene            11491..12606
                     /locus_tag="VFU75_06080"
     CDS             11491..12606
                     /locus_tag="VFU75_06080"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HEU5466439.1"
                     /translation="MNVPMLDLVAQYQTIKKETLDAIHQVIEAQQFIMGPAIPKLEAE
                     VAALSHAKYGIGCASGTDALLLPLKALNLKPGDEVITTPFTFFATAGTIHNAGGTPVF
                     VDIDPDTFNIDPAAVEAAITPRTRAIMPVHLYGQMAPMERLLAIAAKKGIPVIEDAAQ
                     AIGARRKIDGTWRMAGELGWATGYSFFPSKNLGAWGDGGMIVTSDEQGAERLKKLRLH
                     GGAKQYHHEEVGTNSRLDTLQAALLLAKLPHLAGWSAKRREHAASYTKAFAGLGAVRA
                     PVVDPANEHIFHQYTLRVERRDELQAHLKEKGIGHAVYYPIPLHLQPCFAHLKYAVGR
                     LPRAERASHEVISLPIYPELTDAQKNAVVDAVKSFYK"
     gene            12603..13607
                     /locus_tag="VFU75_06085"
     CDS             12603..13607
                     /locus_tag="VFU75_06085"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5466440.1"
                     /translation="MSRLKIGVIGAGAWGRNHVRTVAGLADGELAAVCDTSDATRQKL
                     SRQYPSAVITADVDALLKVCEAVVIAAPAKAHADLALRAIEAGKPCLVEKPFALSVAD
                     AQRVAEAAVARKVPVLAGHLLVYHAAVERLKSLVKSGDLGRIYYLYGLRVNLGQVRAD
                     ENALWSFGPHDVSVALEVLGEAPVRVAAQGHCYLQPRIEDVVFLTMEFASGVLAHVQL
                     SWLDPHKERKLTVVGASKMAVFDDMEAREKLRIYDKGVERPPEYASFGESLSIREGDI
                     FIPRLPNVEPLAAELTHFVRVARGTEAPRAGAKEGVQVVQVLEAASRSLAAGGTPVTL
                     "
     gene            13604..14908
                     /locus_tag="VFU75_06090"
     CDS             13604..14908
                     /locus_tag="VFU75_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HEU5466441.1"
                     /translation="MSPAATPVDALIAKARDRSAVFGIVGLGYVGLPLAMELVRAGYR
                     VIGFDVNQRTVDGLNAGKSHIQDVPTQAVADAVKAKKFSATTDLTRMGEPDAISICVP
                     TPLSKTKDPDVSYVLAATESVKKTLRKGQLIILESTTYPGTTRELMLPALESTGMKVG
                     TDFFLAFSPERVDPGNPKYNTRNTPKVVGGITEHCRAATIALYQPAIDTLVPVSNPEA
                     AELVKILENTFRSVNIGLANEMAIVCDKLGVDVWEVIEAASTKPFGFMKFTPGPGVGG
                     HCIPLDPHYLAWKMRTLNYKTRFIELAGELNAEMPEYWVTKVANGLNDQGKAVRGSKI
                     LVIGVAYKKDIDDIRESPALDVIKLLEQRGAHVSYHDPHIATLHEDKLDLKSVPLTAD
                     ALKSADCVIIVTDHTAVDYQLVAKSARLVVDTRHVLPRGSRS"
     gene            14905..16224
                     /locus_tag="VFU75_06095"
     CDS             14905..16224
                     /locus_tag="VFU75_06095"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HEU5466442.1"
                     /translation="MNVGVVGTGYVGLVVGACLAETGNDVVCADVDGRKIDRLKRNEL
                     PIYEPGLEPLVERNQKDGRLAFTTDVAGAVERSDIIFIAVGTPPDEDGSADLQHVLAV
                     ARTIGQHMNRSKIVITKSTVPVGTAEKVRAEIAKHTRTPFHVCSNPEFLKEGAAVDDF
                     MKPDRVVVGVDADETARALEELYAPFVRTGNPLIFMDIPSAEMTKYAANAMLATRISF
                     MNQVARLCEAVGADVSLVRKGIGSDKRIGPAFLFPGPGYGGSCFPKDVKALIKTGDDR
                     GVSLDVLKAVEAANDRQKMVLFDKLSKHFPQGLRGRTVAIWGLAFKAETDDVRESPAL
                     VLAEALLGAGVNVRVHDPEAMQTARMQLGDRVTYAPTAYAALEGAEALAIVTEWREYR
                     NPDFSKMKGVMRQALIVDGRNLYEPQRLAGLGFTYDSLGRQLACASS"
     gene            16209..17144
                     /locus_tag="VFU75_06100"
     CDS             16209..17144
                     /locus_tag="VFU75_06100"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HEU5466443.1"
                     /translation="MRLLVTGAAGFLGSHLSDALIARGADVVGMDNFITGNRANIAHL
                     TGNKAWTFIEHDVTKYIDVSGELHGILHFASPASPIDYLELPIQTLKVGSLGTHNALG
                     LAKAKGARFFLASTSEVYGDPLVHPQTEGYWGNVNPIGPRGVYDEAKRFAEAMTMAYH
                     RYHKVDTRIIRIFNTYGPRMRPHDGRVVSNFIVQALAGEPLTVYGHGDQTRSFCYVDD
                     LVEGIIRLFERGTEDPVNIGNPNEFTVRQLAERVIALTGSRSTIVERPLPTDDPKVRK
                     PDISLAKSRLDWEPKIALDEGLRRTIDYFQSLKKA"
     gene            17141..18115
                     /locus_tag="VFU75_06105"
     CDS             17141..18115
                     /locus_tag="VFU75_06105"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU5466444.1"
                     /translation="MTAKWVVTGAAGFIGSHVCDRLVGRGDEVIGIDNFDPFYARDIK
                     ERNLAGVQGSKTFRLLDLDCATTDLPWSGVQGVVHLAAKAGVRPSLDDPVAYVEANVR
                     ATARLLDGARRAGVKRMVLASSSSVYGNDTPAPFKEDATADAPISPYAASKRGAELMA
                     RAFAHLYGMRIACVRYFTVYGPRQRPDLAIHRFTRAIARDETIRMFGDGSAERDYTFI
                     SDGVDGTLAALDWTGRSTGADRGEARVFNIGGGERVRLDRLIDLIGRALKRSPRIERA
                     PMQAGDVELTSADLSRSAAELGYRPRVGIEEGIREFVTWYEGAYGRQS"
BASE COUNT         3128 a         5934 c         6310 g         2857 t
ORIGIN      
        1 cggggcggca tgctgaacgc ggtcgggctg gccaatccgg ggctggaaga agtggcggcg
       61 cacgacctgc cctggctgct cgatcgggtc aagcgcgcgc gggtgctcgt gaacgtggcg
      121 ggtgccaccg tggaggacta cgtgaaggtg ctcgagcgcc tcggtggtat cgaccgagtc
      181 accgcgttcg agatcaacgc gtcatgcccg aacacgtcgg ccggcggact cgaattcggt
      241 gccgacgagg cctcgctcgc ccacctggtg cgccggtgcc ggggcgcgac gacgaagccc
      301 ctcgtcatca agctctcgcc ggtcctcccg gatatcgcgc acgctggcga ggtcgcccgt
      361 gatgccggag cggatgggct gagtctcgtg aacacgatgc cggccacgct cgagggaagg
      421 ttggggaacg ggttcggtgg gatgagtggc ccggcgctgt tgccggtggg cgtgctggcg
      481 atccggaagg tccgtgcccg cgtcgccctt cctcttatcg gtgtcggggg cattcggacc
      541 ggtgcggacg cgcgggagta cctgcatgcc ggcgccgacc tcgtcgcgat cggcaccgcc
      601 gcgctggccg acccgagggt ccccgaacgc atcgtgagga gcctgaacgg tggctgagct
      661 cgtcgtcgct ttcgatctgc ccaccggcaa ggaggcgctc gccctcgcgt cgcgcctgcc
      721 cggactcggt tgggccaaga tcggtccggt gctctacgtc cgcgaagggc cggcgctggt
      781 gcgcgagttc actgcccgcg gcatccgggt ctttctcgat ctcaaatggc acgacattcc
      841 gaacacggtc gcgggggccg tcgagggggc ccgcgagctc ggcgtgtcgc tggccacggt
      901 gcattgcctg gggggggcgg cgatgctgtc ggcggccggg aaggcggccg gcgcgcaggg
      961 gaccaccggt gccatttccc tggtcggggt cacggtactg acgtcccacg atgccggggg
     1021 gttcgagggg gtcgtggggc ggggggtacc cgacctgggc ctcgaggtcg tgcggctggc
     1081 ccggctggca cgggatgcgg ggttacgggg ggtggtggcg tcgggccacg aagttgggct
     1141 gctccgggaa gccctggggg ctgaaccttg gctggtggtg cccggcatca gagcgcctga
     1201 tgcccccgcg gacgatcagg cgcggacggt cactcctggg gaggcggtgc ggcggggggc
     1261 cacgcatttg gtggtgggac ggccgattac gggggccaaa gatcccttat cagctttccg
     1321 gggagtgctg gaggctatga ccacgtaatc gcgccccttg cgtgaactta cgagcccggg
     1381 tattgttcaa gactcaacca gaatctttgc cgggagacgg tcttttgaag gtgagaacga
     1441 gcgtcaagcc gatctgcgag cactgcaagg tgatcaagcg ccggggtgtg gtccgggtca
     1501 tctgcaagcg gaatcccaaa cacaaacagc gtcagggcta agccgatggc acgaatcgcc
     1561 ggcgtggacc tgccacgcga caagaagatc cagttcgcgc tcccctacat ctacggagtg
     1621 gggccgtcga acgccaagcg cattctcgcc gagacggggg tgagcccgga cgcgcgcgtg
     1681 cgcgacctca gggacgcgga agtcgcgcgt ttgcggcagg tgatcgagcg ggactacaag
     1741 gtcgaaggtg cgctgcgcac ggaagtggcg atgaacatca agcgattgat ggacatcggc
     1801 acgtaccgcg gcgggcggca ccgcaagaat ctgccggtgc gcggccagcg cacgcacacc
     1861 aacgcgcgca cgaagaaggg tccccggcgc gccatcgcgg gcaagaagaa gccctctctg
     1921 aagaaataaa tggctcaggc gaaagctgcg ggcgccaagc gcgccaagaa agtcgtcgaa
     1981 gcggagggcg tggcgcacat cacggccacg ttcaacaaca cgctcatcac catcaccgac
     2041 tcccatggca acgccatcac gtggggctcg gcgggcaagg ccggcttcaa gggctcgaag
     2101 aagtccacgc ccttcgccgc caccgtggcc gcggagcagg cggcgcgcga agcgctgtcg
     2161 ctcggagtga agcgcgtgca tgtgcgggtg cagggtcccg gctccggccg cgagtccgcc
     2221 atccaggcgc tcgccaccgc cggcctgcag atccgctcca tccgcgacgt gacaccgatt
     2281 cctcacaatg ggtgccggcc gcccaagaag cggcgggtct gatccatggc acgctatacg
     2341 ggtccggcct gcaggctctg ccgtcgtgag ggcaccaagc tctttctgaa gggcgcgcgc
     2401 tgtctcaccg agaagtgcgc gattgaacgc cgcgcgtacg ccccgggcca gcatggccag
     2461 ggcacgggcc ggcgccggaa ggcatccgag tatgccaagc agatgcggga gaagcagaag
     2521 gtgaagcgca tctacggact ctcggagcgc cagttccgga acgtgttcga acgcgtgctg
     2581 cgtgagcccg gtgtcacggg cgaggcgctg ctcatcgcgc tcgagtcgcg gctcgacaac
     2641 atcgtgttcc ggatggggtt cgcctcgtcg cgccggcagg cgcggcagtt ggtccgccac
     2701 cggcacattc aggtgaatgc tcggaccgtc gacattccgt cctaccggat ccgtcccggc
     2761 gatgaagtga aggtcgccga cgcgagccgt gagctggccc tggtgaaggt ggccgccgag
     2821 ctcgccggcc ggggacagcc ggtcagctgg attcaagtgg acagcgacaa ggccctcggc
     2881 aagatgaccg agcggcccac ccgcgacgcc atcccggtcg cggcgcaaga acagctcatc
     2941 gtcgagctct actccaagtg atgacgacga ctctcgattt gaccggattg gtccgcccgc
     3001 acgtggtcga aatgaccaag cgggaggata cgcccaacgc cgccgagttc cggctgcagc
     3061 cgctcgagcg gggctacggg tacacgctgg ggaattcgct ccgccggatg ctgctctcct
     3121 cgctccgcgg cgccgccgtg tgggcgttcc gcctcgacgg cgtggtgcac gagcaccaga
     3181 ccatcccggg cgtggtggaa gacgtgcatc agatcattca gcgtctgaag cagctcaccc
     3241 tcgtgctcgc gcccgacgcg gacgaggcgg tgctgcacat caagcgcgag aaggccggcc
     3301 ccgtctacgc ccgcgacatc gaagcgcatg gcgcggtgac ggtggtcaat cccgatcagc
     3361 tcctgttcac cgtgcaggac gacaaggaaa tgaacgggga gctgtacgtg aacaagggcc
     3421 gcgggtacgt ggaagccgag cagcatccgg tcgaccgcac cctgtcggtg gacgtggtgc
     3481 ggatcgactc gatctacaat ccggtccgcc gcgccaattt caccgtggcc gagacccgcg
     3541 tgggtcagcg caccgactac gaccggctca cgctgtcggt cgagaccaac ggcaccatca
     3601 gtcccgagga cgcggtggcg tacgccgccg cgctcgcgca ggagcacttc cgctacttcg
     3661 tcgacttcgg caaggtcccg atggtggccg ccgaagcgcc gagcgcgggg ggcggctccg
     3721 cggcgccggg ccggctcaag gagctccttg accggagcat cgatgacgtc ggcttgacgg
     3781 tccgctcggt caactcgctc aagaattcca acatccgcac ccttggcgat ctggtgcagc
     3841 acgccgaagc cgacttgctc aaggtcaaga acgtcggcga caaggcgctc ggggagatcg
     3901 cggatctgct gcggcgcgag ggcctgaact tcggcatgcg gtacgaggaa accggcacgg
     3961 gtgacgtgcg ggtcctcgag gccggcgtgc cgccgatggc ccaggtcgcg agcggagacg
     4021 agtaatgcgg caccgcgcga agggacggca gctctcccgc acgtcgagcc acaagaaggc
     4081 gacgctgaac aacatggcga cctccctctt ccggcacgac cgcatcgtca ccacggaagc
     4141 caaggcgaag gagctccggc cgttcgcgga gcacctgatc accctggcgc gccggggtga
     4201 tctgcacgcc cgccggctgg tggagcggcg gatcgcggac aaggaagtga gtcaccgcct
     4261 gttcgccgag ttgggcaagc gattcgcggc ccggccgggt ggctataccc gcatcgtgaa
     4321 actgggacat cgcgtcggtg atggtgccga cgtggcccgg atcgaactgc tgagtgagtg
     4381 agcgagcggc ggggcgttcg gtgcaacaga aggggacccc gacgggtccc cttttctctt
     4441 caggcggctt tggttacgcg gtgcgacttg atgcagcggg tgcagacccg gatacggcgg
     4501 gtggtgccgt ttacgacggc gcgcacgcgc tgcaagttgg gaaaccagcg gcgattcgac
     4561 ttgttgttcg cgtggctgac gttgtgcccc gtcgcggggc ccttgccaca cagatcacac
     4621 acacgagcca tggtcggtcc agcgaatgag ttccactgca cgcacacccc ggggatcggg
     4681 gcctgagcag gggcggaaac ctaacggggg gtccgaggcc gggcaacccc gccaggccca
     4741 gccgaagaca gccgtcatta cgggcgtcac cggccaggac gggtcctacc tcgcggagct
     4801 gctcctggag aagggctacg aggtggtcgg cgtggtccgc cggacctccc acgactccta
     4861 cgaacggatc ggccacctcc tcgaccgggt ccacatcgtg cccgccgacc tgctcgacca
     4921 gcactcgctc accgcggtga tccgggacgc caagcctcac gaggtctaca acctcgcggc
     4981 gcagagcttc gtccccacgt cctggagcca gcccgtgctc accggcgagt tcaccgcgct
     5041 cggcgtcacc cggctgctgg aagcggtccg gctggcgcat ccggaggcgc gcttctacca
     5101 ggcctcgtcc tccgagatgt tcggcaaggc gcaggagacc ccgcagaagg agacgacgtc
     5161 cttctacccg cgctcgccct acggcgtggc gaaggtgtac gggcactgga tcaccgtgaa
     5221 ctaccgtgag tcctacggcc tctacgcggt ctccgggatc ctgttcaacc acgagtctcc
     5281 gcgccgcggt ctcgagttcg tgacgcggaa agtcacgcac gccgtcgccc ggatcaaacg
     5341 ggggcggggc accgaagtcc gcctcggcaa cctggatgcc cgccgcgact ggggctatgc
     5401 cggcgattat gtcgacgcga tgtggcgcat gctccagcag ccgatgcccg ccgatttcgt
     5461 gatcggcacc ggcgagacgc acacggtccg cgagctctgc gaaatcgcct tcggacacgc
     5521 cgggctcgac tggcagaggc acgtcgtgat cgatccgaaa ttcgtgcgcc ccgccgaagt
     5581 cgatctgctg ctcgccgacc cgagccgcgc gaaggcggag ctccactggg cgccgaagac
     5641 cggcttcgcg gacctcatca agatgatggt ggacgccgat ctggaacgcc tggcgtgaag
     5701 gttctggtca cgggtgcgga cgggttcgtg ggccggtgga tgatccgccg gctcctgacc
     5761 gacggccatg aagtgttcgg cgcgctgcgg cccgccgggg ccacccacga ggacgggctg
     5821 agcgaccagg agcgggagtc ggtccgctgg ctgcccctcg agctcacgga cgccgattcg
     5881 gtccggatgg tggtcgacct gccgtacgac gcggtgattc atctcgccgc cgtggcctcg
     5941 gggccggaag cggcgcgcga tccgggatac gcgtggaccg tgaacgccgc cgggacggcg
     6001 cgcatcgcgt acgtgctggc ggaagcgcga cggaagggac gggccgatcc ggtcttcctt
     6061 ctggtgtcga ccggcgaggt gtatggacga gggctcgacg gaaagaacgg ccgcccgcgc
     6121 ctcgaaaccg acctgctcgc gccctgctcg ccctatgcgt cgagcaaagt cggcgcggag
     6181 accgcggccc tcgaagcgtg gcggcgcacg ggcttgcgcg tggtggtggc gcgcgcgttc
     6241 gcgcacaccg gccccggaca ggacacacgg ttcgtggtgc cggccttcgc gcagcgcctc
     6301 cgctacgccc ggcagatcgg cgccccggcg gtaaaagtcg ggaacctgga gccggtcagg
     6361 gagtttctgg acgtgcggga tgtcgtggac gcatataccc ggctcctcgc caagggcgtg
     6421 ccgggggagg cgtacaacgt ggccagcggg caggggatcg agctgcgcca gctgttcgac
     6481 aaggtggcgg cgcggctcgg cgtgaaggcg attcccgaaa cggatcccga gctcatgcgg
     6541 ccagccgaca ttccgaatct ggtcggggac ggatccaagt tgcgggcggc gaccgggtgg
     6601 gcgccgacct tttctctcga cgacacgctg cgggatacgg tggatgccca agcgcactga
     6661 cctgaagcgc atactcctca tcggctccgg ccccatcgtc atcgggcagg gggccgagtt
     6721 cgactactcg gggacgcagg ccgtcaaggc gctgcgcgaa gagggctatg aagtcgtcct
     6781 ggtgaactcg aacccggcga caatcatgac cgatcccgag ctcgccgatc agacctacgt
     6841 ggagccggtc accccggcct gggtccgcaa ggtgatcgag aaggagcggc ccgatgcgct
     6901 cctgccgacc atggggggcc agacggccct gaacgtcgcg atggccctgg cccaggacgg
     6961 cacgctcgcc gagttcggcg tgcagctgat cggcgccaat gcccgggcca tcaagatggc
     7021 cgaagaccgc gccgagttcg cgatcgcgat gcggcgcatc ggcctcgaga cgccgctcgg
     7081 ccggaccgtg agctcccgcg aggaagccct cgccgccgtc gcggagaccg gctaccccgc
     7141 catcatccgt ccgtcgttca cgctcggcgg caccgggggc ggcgtcgcgt acaaccgtgc
     7201 cgagttcgag gagctggtgg acacggcgct cgagctctct cccgtgcatt cgacgctcat
     7261 cgagcgctcg gtgatcggtt ggaaggaatt cgagctcgag gtgatgcggg atgggcgcga
     7321 caacgtcgtg atcgtctgct cgatcgagaa catcgacgcg atgggggtcc acaccggcga
     7381 ttcgatcacg gtcgcccccg cgatgacgct gaccgaccgc gagtaccaga agatgcgtga
     7441 ctcggccatc gccatcatcc gggagatcgg cgtcgaggcc ggcggctgca acatccagtt
     7501 cgccgtcaat cctgccgacg gcgagatgct ggtgatcgag atgaaccccc gggtgtcgcg
     7561 gtcgtccgcg ctcgcgtcga aagcgacggg gttccccatc gcgcggatcg gggccaagct
     7621 cgcggtgggg tatacgctcg acgagctgcc caacgacatc acccggacca cgcccgcgtc
     7681 gttcgagccc gtgctcgatt acgtggtcgt gaaagtcccc cgcttcgcgt tcgagaaatt
     7741 cccgcgtgcc gatttccacc tcacgaccca gatgaagtcg gtcggtgagg cgatggcgat
     7801 cggacggacc ttcaaggaag cattccagaa ggggctgcgc gccctcgaga ccggccggac
     7861 cggttggagc ctcggcgcca ccctggccga tgaccggttg accggcgact caccggaaga
     7921 catccgggtc gcgctccgcg tgcccacgcc ggagcgggtc tttcagatca agcgggccct
     7981 gctcgccggt ctcacggtgc cggacatcgc gggagcgtcg ggaatcgatc cctggtttct
     8041 ccatcagttc gccgagttga tcgcggccga gcgggcgtac gcggccgatc cgaagcccgg
     8101 tgcggcgggc atccgccgca tgaagcgtct gggattctcc gaccggcagc tcgcggcgct
     8161 gaagggagag accgaggccg cggtgcgcga gcgccggtgg gcactcgaca tccacccggt
     8221 ctacaagacc gtcgatacct gcgcgggcga gttcccgtcg agcacgccgt atctctactc
     8281 ctgctacgac cacgagaacg agtcggaacc gctgggagag cagggcatcg tgattctcgg
     8341 atccggaccg aaccggatcg ggcagggggt cgagttcgat tactgctgcg tccgcgccgg
     8401 actcgcgttc cgcgagctcg gttacaagac catcatgatc aactcgaatc ccgaaacggt
     8461 ctccaccgac ttcgacatct ccgacaagct gtacttcgag cctctgacct tcgaggacgt
     8521 gctcgagatc atccactggg agcggcccaa gggcgtggtc gtgcagctcg gcgggcagac
     8581 gccgctccgg ctcaccaagc cgctcgaggc cgccggggtc cacattctcg gcaccacccc
     8641 ggacgccatc gacatcgccg aggatcgcga acgcttcgac gccctggccc ggcgcctggg
     8701 tgtgacccag cccgcgaacg ggatggcgcg ctcggtggac gaggccgtga aggtggccga
     8761 gcgcatcggg ttcccggtgc tgatccgccc atcgtacgtg ctcggtgggc gggccatgga
     8821 aatcgtgtac gacgtgggct ctctccggca gttcttcgag gtggcggccc gcgtggcgcc
     8881 ggaacatccg gtgctcatcg accggttcct ggaggacgcg ttcgaagccg acgtcgacgc
     8941 catttccgac gggaagcgga cggtggtggg cggcgtgatg cagcacatcg aggacgccgg
     9001 ggtccattcg ggggactcgg cctgtgtcct tcccccgtat ctcatcggcg accggcaggt
     9061 ggcggagatg cgcaagcaca ccaaggcgtt cgccgaggcg ctgggggtgg tcggcctgat
     9121 caacgtccag tatgcggtca aggacgggac cgtctacgtc ctggaagtga acccccgggc
     9181 ctcccggacc atcccctttg tcagcaaggc gaccggggtc tccctggcca agctggccgc
     9241 ggcggtcatg gtggggcatg gactggacga attgggggtc cccgatgagc ttcccctccc
     9301 cggagtggcc gtgaaggagg ctgttttccc cttcaccaag ctccccggcg tagatagtgt
     9361 gctgggcccg gaaatgcggt ccaccgggga agccatgggg ttcgccgaca gcttcggcat
     9421 ggcgttcgcc aaggcacaga tcgcggcgga ctcggggttg ccgctggacg gggcgatctt
     9481 cgtgaccgtc aacgattcgg acaagccgac cgtcgtgccg atcgtccggc ggtttcatga
     9541 gctggggttc cggattctgg cgacggcggg aacccaggcg tacctgcggg cccggggcgt
     9601 gcccgcggag tcggtgcgca aggttcacga gggacgaccc aatgcgatcg atctcctcgt
     9661 ctccggcgac atccagctcc tgatcaatac gccgctcggg aagttcacac agcaagacga
     9721 ctacgcgatc cgccgcgccg cattggtgca tcggatcccg tacaccacca cgatgtccgc
     9781 ggccagcgcc gcatgcgacg cgatcatcgc actgcgcagc cggaccggca gcgtgaggag
     9841 cttacaggca tggcacccaa aacccctcgt cggaaatcag ccgcgtcagg taccaaagac
     9901 gcccgcccca agtcccggag cagcggtaag ggcgtagcga aggccgccgc ggcgaaaccg
     9961 gtcaaggcac caccggcgcc tccgcagccc cccaaggcga agttccgggg gcggctgctg
    10021 gagcaggagc cgctcgcccg gtataccacg taccgcatcg gcgggccggc gaagtacctg
    10081 ctgttcccgg ccgatgtcga cgacgtgcag cggggcctcg agatggccca gaaccgcaat
    10141 ttgccctggg tggtcctcgg actcggttcg aacgtcctgg tgaaggactc cggattcccg
    10201 ggcgtcgtga tccgtctggg gaagggtctc gaccgcttcg agatgaaggg cgccacggcc
    10261 atcgtcggcg cggggatgcc catcccgatt ctggcccggc gtacggccga ggcgggcttc
    10321 gtcggcgtgg aacggttcat cggcatcccg gggacggtcg ggggcggcat ctacatgaac
    10381 gccggatgtc atggcgccga gttctccgag gtggtgaccg agatcaccgt gatggacgcc
    10441 aagggcaagg tcaagcagat cgcccggaag cagatctcgt tcaagtaccg ggcctccaac
    10501 ctggggccgg tgatcgtcct cgaagccaag ctggtcctcg gcgaggagtc gcccgccaag
    10561 ctcaaggagc tccagagccg cctcttccgc tggcggaagg cgggaacgcc ctatgatcag
    10621 ccctgctgcg ggtcggtgtt caagaatccc tcgctcacgc ggaccgcggg catgctcatc
    10681 gacgaagccg gcctgaaggg gcacacggtg ggcggcgcgc aggtgtcgaa tctccacgcc
    10741 aactacttca tcaatctggg gaatgccacg gcggccgacg tgcagaagct gatggaccac
    10801 gtgaaaaaga ccgtccagaa gaagacggga gtggagctcg agccggaagt gcgggtgatc
    10861 ggctgatggc cgactactac gttcatccct cgagttttct ggatgaaggc gccgtcgtag
    10921 gtgccggtac gaagatctgg cacttctgtc acgtgatgaa gggcgccgtg atcggtgagc
    10981 gctcgagtct cggtcagaac gtcgtcgtga tgccgggcac cagcatcggc aacaacgtga
    11041 agatccagaa caacgtgtcc atctacgagg gggtccacct cgaggacgac gtcttctgcg
    11101 gtccttcctg cgtgttcacg aacgtcctga acccgcgaag ccacgtctcc cggaagcacg
    11161 aataccgcca aaccctcgtg aagcggggcg cgtcgatcgg cgccaacgcc acgatcgtct
    11221 gcggcaccac gctcggcgag ttcgccttca tcggggcggg cgccgtggtg acgtccgatg
    11281 tccccgcgta tgggttgatg gtcggagtcc cggcccgccg ggtgggctgg atgtgtcagt
    11341 gtggcgaacg gctctcgatc tcggcgggcg cggcgcagtg cgccgcctgc ggcgccgagt
    11401 acgccgagtc gggtgggacc ctccgcatta ccaagccgcc gcgcccggcg tgacgggacg
    11461 gccgtttcaa ttgaacccct ggagaatttc gtgaacgtac caatgctgga cctggtggcc
    11521 cagtaccaga ccataaagaa ggaaacgctc gacgccattc atcaggtgat cgaggcccag
    11581 caattcatca tgggcccggc catccccaag ctcgaggccg aggtcgccgc cctctcgcat
    11641 gccaagtacg gcatcggctg cgccagcggg accgacgccc tcctgctgcc gctgaaggcc
    11701 ctgaacctga agccggggga cgaggtgatc accaccccgt tcaccttctt cgcgacggcg
    11761 gggaccatcc acaacgccgg cggtaccccg gtgttcgtgg acatcgatcc cgatacgttc
    11821 aacatcgatc cggccgccgt ggaagcggcc atcacgccgc ggacccgggc catcatgccc
    11881 gtgcatctct atggccagat ggcgccgatg gaacggttgc tggccatcgc cgccaagaag
    11941 ggtatcccgg tcatcgagga cgccgcgcag gccatcggcg cccgccggaa gatcgacggc
    12001 acgtggcgca tggcaggaga gctggggtgg gccaccggct attccttctt ccccagcaag
    12061 aacctcggag cctggggtga cggcgggatg atcgtcacct ccgacgagca gggggccgag
    12121 cgcctcaaga agctccggct gcatggcggc gccaagcagt accatcacga agaggtcggc
    12181 accaactcgc gcctcgacac gctccaggcg gccctgctcc tggccaagct cccgcatctc
    12241 gcgggctggt cggcgaagcg gcgggaacac gcggcttcct ataccaaggc cttcgccggc
    12301 ctgggtgcgg tccgcgcgcc cgtggtcgac cccgccaacg agcacatctt ccatcagtac
    12361 acgctccggg tggagcgccg ggacgagctg caggcgcatc tgaaggagaa gggcatcggg
    12421 cacgcggtct attatccaat tcccctgcat ctccagccct gcttcgccca cctcaagtac
    12481 gccgtgggcc ggttgccccg ggcggagcgc gcgtcgcacg aagtgatctc gctcccgatc
    12541 tatccggagc tgacggacgc gcagaagaac gcggtcgtcg atgcggtgaa gagcttctac
    12601 aagtgagccg gctgaaaatc ggcgtcatcg gcgccggcgc ctgggggcgc aatcatgtcc
    12661 gcaccgtggc gggactcgcg gacggcgagc tggcggcggt gtgcgacacc agtgacgcca
    12721 cccggcagaa gctgtcccgg caatatccgt ccgcggtgat cacggcggac gtcgatgcgc
    12781 tgctcaaggt gtgcgaggcc gtcgtcatcg ccgcgcccgc taaggcgcac gcggacctgg
    12841 ccctgcgggc catcgaggcc ggcaagccct gcctggtcga gaagcccttt gcgctgtccg
    12901 tggcggacgc gcaacgggtg gctgaggcgg ccgtagcacg gaaggtgccg gtcctcgccg
    12961 gtcacctgct ggtataccac gcggcggtcg aacggctcaa gagtctggtg aagagcgggg
    13021 acctcggcag gatctattat ctatacggcc tcagggtgaa cctggggcag gtccgggccg
    13081 acgaaaacgc cctctggagc ttcggtcccc acgacgtttc cgtggccctg gaagtgctag
    13141 gggaagcacc cgttcgggtg gccgctcagg gccactgcta cctccagcct cgtatcgagg
    13201 atgtggtctt cctgaccatg gagttcgcga gcggcgtcct ggcgcacgtc cagctgtcgt
    13261 ggctcgatcc gcacaaggag cggaagttga cggtggtcgg cgcgtcgaaa atggcggtct
    13321 tcgacgacat ggaagcccgc gaaaagctcc gcatctatga caagggcgtg gagcggccac
    13381 ccgagtatgc gtcattcggt gaaagtctgt ccattcggga gggggacatc ttcattcccc
    13441 gcctccccaa cgtcgagcct ctggccgctg agctgacgca ctttgtgcgc gtcgcccgcg
    13501 gcacggaggc cccccgcgca ggtgccaagg aaggtgtcca agtggtccaa gttctcgaag
    13561 cagcatcccg ctcgctcgcg gccggcggca cgccggtgac cctgtgagtc ccgccgccac
    13621 gcccgtcgac gcgctgatcg ccaaggcgcg cgaccggagc gcggtcttcg gcatcgtcgg
    13681 gctgggctac gtcggtctgc cgctcgccat ggagctggtg cgggcggggt accgcgtcat
    13741 cgggttcgac gtcaaccagc ggaccgtgga cggcctgaac gccgggaagt cacacatcca
    13801 ggacgtgccg acccaggccg tcgcggacgc ggtgaaggcc aagaagtttt ccgccacgac
    13861 cgatctgacc aggatgggcg agcctgacgc gatctcgatc tgcgtgccca cgcccctgtc
    13921 gaagaccaag gatccggacg tcagctacgt cctggcggcc accgaatcgg tcaagaagac
    13981 cctgcggaag ggccagctga tcattctcga gtccaccacg taccccggca cgacccgcga
    14041 gctgatgctt cccgcgctcg agtcgacggg catgaaggtc ggcaccgact ttttcctggc
    14101 gttctcgccc gagcgcgtgg atccgggcaa tccgaaatac aacacgcgca acactccgaa
    14161 ggtggtgggc ggcatcacgg agcattgccg ggccgctacc atcgccctgt accagcccgc
    14221 catcgacacg ctggtgccgg tgtcgaatcc ggaggcggcg gagctggtga agattctgga
    14281 gaacacgttc cggtccgtga acatcgggct cgcgaatgag atggccatcg tttgcgacaa
    14341 gctgggcgtg gacgtgtggg aagtgatcga ggcggcgtcc accaagccct tcggattcat
    14401 gaaattcacg ccggggccgg gcgtcggcgg tcactgcatt ccgctcgatc cgcactatct
    14461 cgcgtggaag atgcggaccc tgaactacaa gacccgcttc atcgagctgg ctggtgagct
    14521 caacgccgag atgcccgagt actgggtgac caaggtggcg aacgggctca acgatcaggg
    14581 caaggcggtg cgggggagca agatcctcgt catcggggtc gcctacaaga aggacatcga
    14641 cgatatccgc gagagtccgg ccctggacgt catcaagctc ctggagcagc ggggggcgca
    14701 cgtctcgtac cacgatccgc acattgccac gctgcacgag gacaagctcg acctcaagtc
    14761 ggtgccgctg accgcggacg cgctcaagag cgccgactgc gtgatcatcg tgaccgatca
    14821 caccgcggtg gattaccagc tcgtggccaa gtcggcgcgc ctcgtcgtgg atacccgtca
    14881 cgtcctgccg cgagggagcc ggtcgtgaac gtcggcgtcg tcggcacagg atacgtcggc
    14941 ctggtcgtcg gcgcgtgtct cgctgaaacg gggaacgacg tggtctgtgc cgacgtcgat
    15001 ggccggaaga tcgaccggct gaaacggaac gagctcccga tctacgagcc gggactcgag
    15061 cccctggtgg agcggaatca gaaggacggg cgtctcgcct tcaccaccga cgtcgccggg
    15121 gccgtcgaac gctccgacat catcttcatc gccgtcggaa cgccgcccga tgaggacggc
    15181 tccgccgacc tgcagcacgt gctcgccgtg gcccgcacca tcggccagca catgaatcgt
    15241 tccaagatcg tcatcaccaa gagcacggtt ccggtgggga cggccgagaa ggtccgcgcg
    15301 gagatcgcga agcacaccag gacgccgttc cacgtctgct ccaaccccga gtttctgaag
    15361 gaaggcgcgg cggtcgacga cttcatgaag cccgaccggg tggtcgtcgg ggtcgacgcc
    15421 gacgagacgg cccgggcgct cgaggagctc tacgcgcctt tcgtccgcac cggcaatccg
    15481 ctgatcttca tggacattcc gtccgcggag atgacgaagt acgcggccaa cgcgatgctc
    15541 gcgacgcgga tctcgttcat gaaccaggtc gcccggctct gcgaggcggt cggcgccgac
    15601 gtttcgctgg tccggaaggg catcggcagt gacaagcgga tcggacccgc cttcctgttc
    15661 ccgggcccgg gatacggcgg gtcctgcttc cccaaggacg tgaaggcgct catcaagacc
    15721 ggggacgatc gcggcgtgtc cctcgacgtg ctcaaggcgg tcgaggcggc caatgaccgg
    15781 cagaagatgg tgctgttcga caagctgtcg aagcacttcc cgcagggact gcgcggcaga
    15841 acggtggcca tctggggact cgccttcaag gcggagacgg acgacgtgcg tgaaagtccc
    15901 gcgctggtgc tcgccgaggc cctcctcgga gccggcgtga acgtccgggt gcacgatccg
    15961 gaagcgatgc agaccgccag gatgcagctg ggggaccggg tgacctacgc ccccaccgcc
    16021 tatgcggcgc tggaaggcgc ggaggcgctc gcgatcgtga ccgagtggcg cgagtaccgg
    16081 aaccccgact tctccaaaat gaagggtgtg atgcgtcagg cgctgatcgt cgacgggcgg
    16141 aatctgtacg aaccccagcg gctcgccggt ctcggcttca cgtacgacag cctcggccgt
    16201 cagctcgcat gcgcctcctc gtaaccggcg cggcagggtt cctcggttcc cacctctcgg
    16261 acgccctgat cgcgcgcggg gccgacgtgg tggggatgga caacttcatc accggcaatc
    16321 gcgccaacat cgcgcacctc acggggaaca aggcctggac cttcatcgaa cacgacgtca
    16381 ccaagtacat cgacgtctcc ggtgagctgc acggcattct gcatttcgcg agtcccgcga
    16441 gtcccatcga ttacctcgag ctcccgattc agaccctgaa ggtcgggtcg ctgggcaccc
    16501 acaacgcgct cggtctcgcc aaggcgaagg gggctcggtt ctttctggca tcgacctcgg
    16561 aggtgtacgg cgatccgctg gtgcacccgc agaccgaggg ctattggggc aacgtgaacc
    16621 ccatcgggcc ccgcggcgtg tacgacgagg ccaagcggtt cgccgaggcg atgacgatgg
    16681 cctaccatcg ctaccacaag gtggacacgc gcatcatccg gatcttcaat acgtatggtc
    16741 cgcgcatgcg cccgcatgac gggcgggtcg tgtccaactt catcgtgcag gcgctggcgg
    16801 gagagcccct caccgtctat ggccatggcg accagacccg gtcgttctgc tacgtcgacg
    16861 acctcgtcga aggcatcatc aggctcttcg agcgggggac ggaagatccc gtcaacatcg
    16921 gcaacccgaa cgaattcacc gtccggcagc tggccgagcg cgtgatcgcg ctgaccggga
    16981 gcaggagcac gatcgtggag cgcccgcttc ccaccgacga tcccaaagtg cggaagcccg
    17041 acatctcgct ggccaagtcc cgcctggatt gggagccgaa gatcgccctc gatgaggggc
    17101 tgcgccggac catcgactat ttccagagcc tgaaaaaggc gtgaccgcga agtgggtggt
    17161 caccggcgcc gcggggttca tcgggtccca cgtgtgcgac cggctggtgg gtcgtggtga
    17221 tgaggtgatc gggatcgaca acttcgatcc gttctatgcc cgggacatca aggaacggaa
    17281 tcttgccggc gtccagggct ccaagacatt ccggttgctc gacctcgatt gcgcgaccac
    17341 cgacttgccc tggagcgggg tgcagggggt ggtgcatctc gcagccaagg ccggcgtgcg
    17401 cccctcgctg gacgatcccg tggcgtacgt cgaggcgaac gtacgggcga ctgcccggtt
    17461 gctggatggg gcgcggcggg ccggggtgaa gcggatggtg ctcgcgtcgt cgtcgtcggt
    17521 gtacggaaat gacacgccgg cgcccttcaa ggaagatgcg acggccgacg cccctatctc
    17581 accgtacgcc gcgagcaagc ggggggcgga gctgatggcc cgggcctttg cgcacctgta
    17641 tgggatgcgg atcgcctgtg tgcggtattt cacggtgtac gggccccggc agcgccccga
    17701 tctggcgatt caccggttca ctcgggccat cgccagggat gagacgatca ggatgttcgg
    17761 ggacggatcg gccgaacggg actatacatt catatctgac ggggtcgacg gcactctggc
    17821 ggccctggat tggaccgggc ggtcgacggg cgccgatcgc ggggaggccc gggtgttcaa
    17881 catcgggggc ggagaacggg tacggctcga ccgcctgatc gatctgatcg gccgggcgct
    17941 gaagcggtcg ccgcgcatcg agcgggcacc catgcaagcc ggcgacgtcg agctgacctc
    18001 cgccgatctg tcacggtccg ccgcggagtt gggataccgg cctcgcgtgg gcatcgaaga
    18061 gggcatccgg gaattcgtga cctggtatga gggggcctat ggacgccagt cctgagcatc
    18121 tggtcaatca ggcgcgggag cgcttccagc tcggcgacta ctatgggacc attcacctcg
    18181 tcgaggatgt ggtcgccacg ggccgggcgt tcgccgacgc ccaccacct
//
LOCUS       DASUBP010000165        15487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1312422, whole genome shotgun sequence.
ACCESSION   DASUBP010000165 DASUBP010000000
VERSION     DASUBP010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 15487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15487
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1312422"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1332
                     /locus_tag="VFU75_06110"
     CDS             <1..1332
                     /locus_tag="VFU75_06110"
                     /inference="COORDINATES: protein motif:HMM:NF013314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5466445.1"
                     /translation="EMAQAIESYGLTSQRFKTGTPPRIDGRTVDWSKLQRQDGENAGY
                     RFSFFEKVDLPRQVPCWITWAGEPVKEIIKNHLQESALYGGAISGRGPRYCPSVEDKI
                     VKFPDAPRHQIFLEPEGLDTHELYVNGLSTSLPPNVQLEFLHAVPGLERAKMTRPGYA
                     IEYDYYPPTQLTPWLETKVVERLFFAGQINGTTGYEEAAGQGAVAGLNAVRRIRGQEP
                     VIIGRDDGYIGVLVDDLVTRGVDEPYRLFTSRSEFRLLLRQDNALRRLAPLAERLGLL
                     TDSERQVVEARFREEERIREVAGATSLSPEHAPAEVGELKNTEWVANLVKRPGTSLRA
                     LLLAAGVSEPWTDDAIAAAEIELKYEGYLAREKDAAARLAEMTDFALPMDLPYPELKS
                     LSIEARQKLAAVRPASLAQAGRIPGVSPSDLHNLVVETTRWRRRAPRRAAV"
     gene            1504..2319
                     /locus_tag="VFU75_06115"
     CDS             1504..2319
                     /locus_tag="VFU75_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5466446.1"
                     /translation="MTRVIAIANQKGGVGKTTTAVNLAASLATAERKTLLIDADPQGN
                     ATSGVGIERDRITHSLYDVLLDGHPLNDAKFSAVHFPYLDVIAAAQDLVGAEVELVGK
                     PGREQMLSKALAQVRDQYQYVIIDCPPSLGLLTLNVLTAADSVIIPIQCEYYALEGLS
                     QLLNTIKLVQQNFNSALAIDGVLLTMYDARLNLCKQVAEEAKEYFGARMFAVTVPRNV
                     RLAEAPSFGKPIVLYDVQSIGARSYLAVAQELIRRVEKSGAPATAQPLPEVPA"
     gene            2316..3209
                     /locus_tag="VFU75_06120"
     CDS             2316..3209
                     /locus_tag="VFU75_06120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEU5466447.1"
                     /translation="MTETTAKGRRLGRGLEALLGPTREEAEREGSLSELLVADIKPNP
                     FQPRKDVDPAALEELKSSIKQVGLMQPVVVRTAPGGAGGFELIAGERRLRACQALGWE
                     KIPAVTRDVDDRTVLTLALIENLQRDDLSPVDEARGYERLIAEFFLTQQDVAEAVGRD
                     RSTVANALRLLKLPDVVLAMLHEGHLTVGHARALLGLDDPRVVANLAREAVAQGLSVR
                     EVEDRVRGGRMPERRPRLKKGVGPAPEVRRVEDALRRHLGTDVRVSLRAKGKGQLHIA
                     FYSNEDLARLLELILGTPFDG"
     gene            3230..4030
                     /locus_tag="VFU75_06125"
     CDS             3230..4030
                     /locus_tag="VFU75_06125"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU5466448.1"
                     /translation="MTIVVQIDGDLNTRQYRLPFWAFTVGKWGAVLVVVLAALFFAFA
                     GPITRSAARVPGLEREIARLQAENSRVQELATALNHAEASYQGLRRMLGIRAGDAKPG
                     SPAAAAAAAASEDLMQAPDVVARVPGSSPVYEPGPSAPSHWPVDVPGYVTRGQVRPGD
                     QSETHAGIDIAVAVGTQIRASGGGTVVMAGADTSYGLFVLLRHPDGYESMYAHASRLL
                     VRENDEVSAGQVIALSGSTGRSTAPHLHFEIRRGGRSLDPLTLVKEGN"
     gene            4032..4424
                     /locus_tag="VFU75_06130"
     CDS             4032..4424
                     /locus_tag="VFU75_06130"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HEU5466449.1"
                     /translation="MAVFTEKTRGGSEGDSALSIIGTGMRVVGDITADGVVKVEGTIV
                     GTVHAGRQVLVAKGGMVEGDIFAREAIIGGEVRGSIESTERVELQATSVVHGDVATKR
                     LLVQEGGEVNGVIRMGEGAPTESAAATF"
     gene            4592..5344
                     /locus_tag="VFU75_06135"
     CDS             4592..5344
                     /locus_tag="VFU75_06135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEU5466450.1"
                     /translation="MRLPDLTQYLDQYLSTATIGDYPNALNGLQVANRSDIKKIAAAV
                     DVCAATVRMAAEAQAQLLLVHHGLFWTGPRPLVGPQYDRVAGLLSADIALYSSHLPLD
                     LHTEVGNNPVLARKLGIAIKDGFGELHGSTIGVWGEADGTRDEFVARVGKLLGVTPKS
                     LPFGPARVKRVGIVTGAGGSLIGQAAALGLDTFLTGEGVHHSFFDAEELKVNVIYAGH
                     YATETVGVRALAEHLASKFGITAVFLDHPTGM"
     gene            5341..6537
                     /locus_tag="VFU75_06140"
     CDS             5341..6537
                     /locus_tag="VFU75_06140"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5466451.1"
                     /translation="MTPRRVLAVLSLAELCAMAPWFSASAVSGTLTHIWSLSPTGSAW
                     LTVSVQLGFVTGAFISSALALSDLWSARRLFAWSTAAAALATALITVAPNAPTAFALR
                     FATGATLAGAYPPGMKLAAGWYKEGRGMAIGVMVGALTLGSAVPFLIRWVVPSDAWKL
                     VLMVASLSAIAGAVLILRVPNDGPYAAPSPRFSWTAVPRILERPSIRLATLGYLGHMW
                     EIYAMWASVAAFIAASEVARGTSSSTAPLITFLVVGIGAPGCWLGGVLADRWGRVKTT
                     SLAMLISGACSLVVGLVFGASLWLLVPVLLIWGVSVVADSAQFSTAISELAPPEYVGT
                     ALTLQTSLGFLLTCVTILSLPVLAGYIGWRWSMTALAVGPALGIWAMQALGRRPEARQ
                     LAGGLG"
     gene            6627..7514
                     /locus_tag="VFU75_06145"
     CDS             6627..7514
                     /locus_tag="VFU75_06145"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5466452.1"
                     /translation="MIDRPKQVPLAGPVPRKDPSFVVPRAAPETLSVTWIGHSSFLIQ
                     LGGLNLLTDPVWSDRASPVQWAGPKRVSPPGFPIDQLPRLDVVILSHNHYDHLDDRTV
                     RHLVRAHPDARWVTPIGVGAFIRERGARNVSEHDWWDEVDVLGLTLACTPAQHFSGRG
                     FTDRNQSLWCGWALKAGSTRVYFCGDSGFHPRFVEIGRRLGPFDVALMPIGAYEPRWF
                     MSPVHMDPKEAVIAFRDLHRAHDSEKRGVFVPMHWGTFRLTDEPVEEPPLLTAEHWRK
                     AGLPDVNLWRLALGETRTR"
     gene            complement(7511..7777)
                     /locus_tag="VFU75_06150"
     CDS             complement(7511..7777)
                     /locus_tag="VFU75_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466453.1"
                     /translation="MIVDTESLRRRLEDGLRLIGPHHRLRDRPVKFRQIDTRTIEIIY
                     RDVTGIAETEVLDVKRIIGRECFCVVEPQTAETVTVRFVVPVGD"
     gene            complement(7774..8718)
                     /gene="queG"
                     /locus_tag="VFU75_06155"
     CDS             complement(7774..8718)
                     /gene="queG"
                     /locus_tag="VFU75_06155"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEU5466454.1"
                     /translation="MTAKNVGQERRAKTSSEDVKARAIDLGFSAVGVCTLEPSAHADF
                     IDQWLAAGYGGTMTYLNRQAARRKAPQKILPNARCAVVTLTNYYHGPASSARPRVAQY
                     AWSTDYHAVLGQRLEILATEIARLVPGSTSRAYVDAGPVPERELAQRAGLGWIGKNSM
                     LINPELGSFTFIGVVLTDAALEPDLPFELDRCGTCTRCLDACPTGAFAAPRTLDARRC
                     ISYLTIERRGPFTDLEARMTGDWLFGCDVCQDVCPWNERFAPVTTDSALAPRPELVAP
                     APKTFADLDEDEFRRRYHDTPFSRPGLAGMRRNAKATS"
     gene            complement(8715..8981)
                     /locus_tag="VFU75_06160"
     CDS             complement(8715..8981)
                     /locus_tag="VFU75_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466455.1"
                     /translation="MQAPASWPAFYLRLSGRAVLSPRLALDLLRLAWSFRARDWFRRF
                     PFLPLPPREYMRWRMFTAYGEEGAIPPVEDVIRFARWRRETMGL"
     gene            9005..10186
                     /locus_tag="VFU75_06165"
     CDS             9005..10186
                     /locus_tag="VFU75_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5466456.1"
                     /translation="MTLASGRAARPDPSLIPSLARYRSEFPVFADTIYLNTCSLGALG
                     ARSRRKVTEFLDGWEKRGASAWYDVWWEALQELRTRYARLIGAQGSEIALAPNISTAV
                     GTVASALDYRARPKVVVTSLDFPTVAYQWLARRPEGVEVVVVESPDGVTVPVEAIARA
                     VDARTALVATSHVFFTTGAIQDIRAVARAAHAHGALCLIDAYQSVGQLPVDVHDADVD
                     FLVAGGLKWLLGGPGVVFLYVRDALARSLEPTLTGWFGHRDQFAFDPRGLTRHDDARR
                     FELGTPALAAVHAQLGGLEYIEEIGVPVIREASMGLAEDLIARARDRGWKPRVAASPT
                     DRSAIVMLPMPDPHAAVRHLAQAHIIADARPGHVRLSPFFYNVQEDHVAAIEQLAQID
                     H"
     gene            10257..10892
                     /locus_tag="VFU75_06170"
     CDS             10257..10892
                     /locus_tag="VFU75_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEU5466457.1"
                     /translation="MLRIMGEFVEGFDTLHDVRNAVTIFGSARTKTDDAVYEQAITTA
                     KLLAKEGFPVITGGGPGIMEAANRGAQEGNGLSIGCNIELPFEQHINPYVERSINFRY
                     FFVRKTMFVKYSTAFVVFPGGFGTLDEMFEALTLIQTGKVKHFPVVLMGTTYWGGLVE
                     WLRDRVAAEGKIATEDLNLIHPTDDPADAVRWITQARERRTLAHAASTHNP"
     gene            10904..12037
                     /gene="speY"
                     /locus_tag="VFU75_06175"
     CDS             10904..12037
                     /gene="speY"
                     /locus_tag="VFU75_06175"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF001980.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HEU5466458.1"
                     /translation="MTRSQYLRGQRIDPAPISGKETAAELIDNAFLAYNAGRMREGCR
                     LFVERMLAPDCTVGMSLTGAMTPAGLGMSTIIPLIEAGFIDWIVSTGANLYHDSHFGL
                     GLAMHQGTPFADDVVLREEGVVRIYDIFFDYEVLLSTDAYIREVSAGPEFQRSMSTAE
                     YHYLLGGYILEREKKLGIARKSVLGVAHECGVPIYTSSPGDSSIGMNVAEQALAGSKL
                     RYDVSADVNETAAIVYAAKHGDGTSGVLIAGGGSPKNFVLQTEPQIQEVLGISEKGHD
                     YFLQLTDARPDTGGLSGATPAEAVSWGKIDPDKLPGTVVCYTDNTVALPLLASYALAK
                     RQPRPLKRLYDRRAAMMADLMRAYEKARKSRDARAEAATGGRA"
     gene            12057..13529
                     /locus_tag="VFU75_06180"
     CDS             12057..13529
                     /locus_tag="VFU75_06180"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5466459.1"
                     /translation="MGSRTYAAYVLGVLTLINLLNYLDRNVVFAVFEPVKHELHVTDQ
                     ELGWLGSAYIILLSLCSIPLGVLGDLKSRRVVIAWSLGFWSLVTSAAGFVRGFGQLVF
                     CRSLAGVGEAGYSPASQAIIADYYKGRARAFAMGIYSVGTAVGGILGIWLGGVIAARY
                     GWRWAFIPMGIPGLLLAASAARIREVNERAATPVRALLVKWYRTGIRRVVRYVVPVAA
                     LSALGAITAAVLDLTFDLSFDRSMAVFGVFVAGGVAWTVARLVPVAVRRTAGAGDVAA
                     SAFEEFLEAAAVVLRTPTLIWVFVGGAMVTFAVNGLIAWAATFMARVHNMSVPDFSAR
                     FGVWALAGGISGVLAGGKIGDWLQIRWPGGRVVASGAGFILGGPLVAWLVFSGSLRGF
                     APLLFGIYFFYTWYNGPLAAVIFDVVPAAVRASVTGGFVLFSHLAGDALAPPLVGYLS
                     DKTHSIQTAMLILPVAGVVGGFVILVALKTVANDMRRVKR"
     gene            13526..14518
                     /locus_tag="VFU75_06185"
     CDS             13526..14518
                     /locus_tag="VFU75_06185"
                     /inference="COORDINATES: protein motif:HMM:NF027219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HEU5466460.1"
                     /translation="MSRRQTPRPELRCTRCRAELEHFWSFCPNCAKALIWGDTQRETG
                     AECAYCRWMVSDTFSFCPWCGRNIHDRQSSLEPLKAPKGFKYHARCDWGCGGGVMYPM
                     QWCPWCGREQIWRYTHFQNICPHCDKGVDDWMDTCPWCGEDATGRILIPRALTRARRL
                     LIVSRIMDWNYRILLRPGVSGVDPDAPKVIEIDRRYVTAKRRRDEISWNMLTGLILHE
                     LGHSFLYHHWSWTTTGRFRRAFGEVRKAYRVADEHWVDFERRKIATTLPDYVSVYAAT
                     HPQEDFAETFRFYVARRGKLRELFGEFGRKRKGVQLFEKFLVLHDYVRGLRGWR"
     gene            14611..15348
                     /locus_tag="VFU75_06190"
     CDS             14611..15348
                     /locus_tag="VFU75_06190"
                     /inference="COORDINATES: protein motif:HMM:NF016241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HEU5466461.1"
                     /translation="MRVLTPVLACLLAARPLSAQTPDSVKAKTDTTKLSFGLGLVTTG
                     GNTSVTTFNFGNNFSYISRPWVFSEAASMVYGSTHDTVNNDQYHLLLRVDHGLHDGVS
                     AYVFGTFDRDRFAGFASRYQEAAGFAWKAVEHPMDLLTLEAGAQETQQRDLGTGATAS
                     FTAARAAVAYKHGFNKTAYAQEAVEVLPDLQHSKNLRINSATDLVAPLSTVFSIKVGY
                     VIRFDNLPPPGFEKTDRILTTGVQITL"
BASE COUNT         2519 a         5158 c         5073 g         2737 t
ORIGIN      
        1 gaaatggcgc aagccattga atcatatgga cttacatcac agcgcttcaa gaccgggacc
       61 cctccccgga tcgacggacg gaccgtcgac tggtcgaagc ttcagcgaca ggacggcgag
      121 aatgccgggt accggttctc cttcttcgag aaggtagacc tcccgcggca ggtcccctgc
      181 tggattacct gggccggcga gccggtgaag gagatcatca agaaccatct tcaggagtcg
      241 gcgctttacg gcggcgccat cagtgggcgg ggtcctcggt actgcccctc ggtcgaggac
      301 aagatcgtga agttcccgga tgctccgcgg caccagatct tcctggagcc cgaagggctc
      361 gacacccacg aactgtacgt caacgggctc tcgacctccc tgcccccgaa cgtccagctc
      421 gagttcctgc atgcggtccc cggactcgag cgggcgaaga tgacgaggcc agggtacgcc
      481 atcgagtacg actactatcc cccgacgcag ctgactccct ggctcgagac gaaggtggtc
      541 gagcgcctgt tcttcgccgg gcagatcaac gggacgacgg gatacgagga ggcggcgggt
      601 cagggggcgg tcgccgggct gaacgcggtg cgccggatca ggggccagga gccggtcatc
      661 atcggccggg acgacgggta catcggcgtc ctcgtggacg acttggtcac ccggggagtg
      721 gacgagccct atcgcctatt cacttcccgg tccgagttcc ggctcctcct tcgccaggac
      781 aacgccctcc gccgtctggc tcccctcgcc gagaggctcg gattgctgac ggacagcgag
      841 cggcaggtgg tcgaggcgcg cttccgggag gaggagagga ttcgagaggt cgcgggggcg
      901 acgtccctgt ctcccgagca cgcgccggca gaggtggggg agctgaagaa caccgagtgg
      961 gtcgcgaact tggtgaagag gccggggact tcactccgtg cgctcctctt ggcggcgggc
     1021 gtgagtgagc cctggaccga cgatgcgatt gccgccgccg agatcgagct gaagtacgag
     1081 ggctacctcg cccgcgagaa ggacgcggca gcccgactcg cggagatgac cgacttcgcg
     1141 ttgccgatgg atctccccta ccccgagctg aagagtctct cgatcgaggc ccggcagaaa
     1201 ctcgctgcgg tccgtccggc gtcactcgcc caggccggca gaatccccgg tgtctcccct
     1261 tctgacctgc acaacctggt ggtcgagacc acgcgctggc gtcggcgagc gccgcgacga
     1321 gccgcagttt gaggcgagtc gtagcccaca accgctacgg gaaagtatcg gtgtgtttca
     1381 cgtgaaactt tgatccagtt tcacgtgaaa ctgctcttcc cacctgcaga ttacagcagt
     1441 atattcgacc agacggaaat tcaataatta tataactcgt tgttatatat agagttacgg
     1501 caaatgacaa gagtgatcgc aatcgcaaac cagaagggcg gcgtcggaaa aaccacgact
     1561 gccgtgaatc tcgcggcctc tctggcgacc gcggaaagaa aaacgctgct catcgacgcc
     1621 gatccccaag gcaacgcgac gagcggcgtc ggcatcgaac gcgatcgcat cacgcactct
     1681 ctctacgacg tcctgctcga cggtcacccg ctcaacgacg cgaagttctc cgccgtccat
     1741 ttcccctatc tcgacgtcat cgccgcagct caggatctcg tcggcgcgga agttgagctc
     1801 gtcgggaagc cgggccgcga gcagatgctc agcaaggcgc tggcgcaggt gcgggaccaa
     1861 tatcagtacg tcatcatcga ctgtccgccg tccctgggcc tcctcaccct gaacgtgctc
     1921 acggcggccg actcggtcat catcccgatc cagtgcgagt actacgccct cgagggcctg
     1981 tcccagctcc tcaacaccat caagctggtc cagcagaact tcaattccgc cctggccatc
     2041 gacggcgtcc tgctcaccat gtacgacgcc cgcctgaacc tctgcaaaca ggtcgccgaa
     2101 gaagcgaagg agtacttcgg cgcgcggatg ttcgcggtca ccgtgccccg caacgtccgt
     2161 cttgcagaag ccccctcgtt cggcaaaccc atcgtcctgt atgacgtgca gtcgatcgga
     2221 gcccggagct atctcgccgt cgcgcaggag ttgatccggc gcgtcgagaa gtccggcgcc
     2281 ccggcaaccg cccaaccgct cccggaggtg cccgcatgac ggaaaccact gcgaaaggcc
     2341 gtcgcttagg tcgcggactc gaggcgctcc tcggaccgac acgcgaggag gcggagcgtg
     2401 agggcagcct gagcgagctg ctggttgccg acatcaaacc caacccgttc cagccccgga
     2461 aggacgtcga ccccgcggcc ctcgaggagt tgaagtcctc gatcaagcag gtgggtttga
     2521 tgcagccggt ggtggtccgc acggcccccg gtggtgccgg cggcttcgag ctgatcgccg
     2581 gtgagcgccg cctccgggcc tgccaggcct tggggtggga gaagatcccg gccgtcacac
     2641 gcgacgtcga cgaccggacg gtcttgacgc ttgcgctgat cgagaacctc caacgcgacg
     2701 acctctcgcc cgtcgacgaa gcgcggggct acgagcggct gatcgccgaa ttcttcctga
     2761 cccagcagga tgtcgccgaa gcggtgggcc gcgaccggtc gacggtcgcg aacgcgctcc
     2821 ggctcttgaa gcttcccgac gtggtgctcg ccatgctgca cgaggggcac ctcaccgtcg
     2881 gtcacgcccg cgcgctgctc ggtctcgatg atccacgcgt cgtcgcgaac ctcgcccgcg
     2941 aagccgtcgc gcagggactc tcggtccggg aggtcgagga ccgcgtccgc ggcggtcgca
     3001 tgcccgagcg aagaccgcgt ctgaaaaaag gggtgggccc tgccccggaa gtgcgacggg
     3061 tggaggatgc gctccgccgt cacctgggca ccgacgtccg cgtgtcgctc cgcgcgaagg
     3121 ggaaggggca gctccacatc gcgttctact cgaacgaaga tctcgcccgc ctcctcgagc
     3181 tcatcctcgg cacgcccttc gacggatgac gcacgggaag cagcggggcg tgaccatcgt
     3241 cgtccagatc gacggcgatc tcaacacccg ccagtaccgg ctgcccttct gggccttcac
     3301 ggtggggaag tggggcgccg tgctcgtcgt ggttctcgcc gcactgttct tcgccttcgc
     3361 cggtccgatc accaggtccg cggcgcgggt gccgggcctc gagcgcgaaa tcgcgcggct
     3421 ccaggcggag aactcgaggg tgcaggagct cgccaccgcc ctgaatcacg cggaggcgag
     3481 ctatcagggc ctgcggcgca tgctgggtat cagagcgggg gatgcgaaac cgggaagccc
     3541 cgccgcagcc gcggccgcgg cggccagcga ggatctgatg caagcgcctg acgtggtggc
     3601 tcgtgttccc ggctcgagcc ccgtgtatga accggggccg tccgccccgt cccattggcc
     3661 ggtggatgtc ccggggtacg tgactcgcgg ccaggtgcgc ccgggggacc agtcggagac
     3721 acacgccggg atcgacatcg cggtagcggt cggcacgcag atccgggctt cgggaggtgg
     3781 gaccgtggtc atggccgggg ccgacacgtc ctacgggttg ttcgtgctct tgcggcatcc
     3841 ggatgggtat gagagcatgt atgctcatgc gtcgcggctg ttggtccgcg agaacgacga
     3901 agtcagcgcg gggcaggtga tcgccctctc gggtagcacc gggcggtcga ccgctccgca
     3961 tctccatttc gaaattcgac gcgggggccg gtcactcgac ccgctcacgc tggtgaagga
     4021 ggggaactga catggcagtc ttcaccgaaa aaacccgtgg gggcagtgaa ggcgattcgg
     4081 cgctgtccat catcggaacc ggcatgcgcg tcgtcggcga tatcacggcg gacggcgttg
     4141 tgaaggtcga gggcaccatc gtcggcactg ttcacgccgg ccggcaggtt ctcgtggcca
     4201 agggcggaat ggtcgagggg gacatcttcg cccgcgaagc catcatcggt ggcgaggttc
     4261 gaggcagcat cgaatccacc gagcgcgtgg agcttcaggc caccagcgtg gtccatggcg
     4321 acgtcgcgac caagaggctc ctggtccagg agggtggcga ggtgaacggg gtgatccgga
     4381 tgggggaggg tgcaccgacg gagtccgcag ccgccacttt ctaggtcacg aatatccaca
     4441 agcgcacctt gcgcaaattg tggattccct aagtcaattg tgcatatagt cttagcttga
     4501 ccggaatgtg ggaatctccg accccgagtc agggttcgct ggtgttaata gtgtcgtaat
     4561 gatgtcgtct tactgacaaa ccaggggacc gatgagactc ccggatctca cccaatacct
     4621 cgaccaatac ctctctactg ccacaatcgg ggactatccc aacgccctga acggcctcca
     4681 ggtcgcgaac cggagcgaca tcaagaaaat cgccgccgcc gtcgacgtct gtgccgcgac
     4741 cgtccgcatg gccgccgagg cccaagctca gctcctgctg gttcaccacg gactcttttg
     4801 gaccggcccc cggcccctgg tgggtccgca atacgatcgc gtggccggac tcctctccgc
     4861 tgacatcgct ctctacagct ctcacctccc tctcgatctc cacaccgaag tcggcaacaa
     4921 cccggtgctc gcccgaaagc tggggatcgc gatcaaggat ggcttcggcg agctgcacgg
     4981 ctcgacgatc ggggtgtggg gagaagcgga tggcactcgg gatgaattcg tcgcccgggt
     5041 cgggaagctc ctcggggtca ctcccaagtc gctgccattc ggccccgccc gcgtgaaacg
     5101 ggtcgggatc gtgaccggag ccgggggatc gctgatcggg caggccgccg cgcttggcct
     5161 cgacaccttc ctgacgggcg aaggtgtcca ccactccttt tttgacgcgg aagagctcaa
     5221 ggtgaacgtg atttacgcgg gccactacgc cacagaaacg gtcggcgttc gcgccctcgc
     5281 cgagcacctc gcttccaagt tcgggatcac cgccgtcttt ctcgatcacc cgacgggcat
     5341 gtgacacccc gccgggtcct cgccgtcctg agcctggccg agttgtgcgc gatggcgccc
     5401 tggttcagcg cgtcggcggt atcgggcacc ctgacgcaca tctggtccct gtcgcccacc
     5461 ggctccgcgt ggctcacggt gagcgtacaa ctgggattcg tcaccggtgc tttcatctcc
     5521 agcgccctcg ccctgtccga cctctggtcg gcccgccgcc tcttcgcgtg gagcacggcg
     5581 gcagcggcgc tcgccaccgc actcatcacc gtcgctccca atgccccaac ggcgttcgcg
     5641 ttgcgcttcg ctaccggagc gacactcgcc ggcgcctacc ccccggggat gaagcttgcc
     5701 gccggctggt acaaggaagg ccgtgggatg gcgatcggcg tgatggtcgg agcgctgact
     5761 ttgggatcag cggtcccgtt cctcatccgg tgggtggtcc cgtcggacgc ctggaagctc
     5821 gtgctgatgg tcgcgtctct cagcgccatc gccggtgcgg tcctgatcct ccgggtcccg
     5881 aacgacggcc cctatgccgc tccctcaccc cgcttctcgt ggaccgcggt cccgcggatt
     5941 ctcgaacgcc cgtcgatccg cctcgcgaca ctcggatatc tggggcacat gtgggagatt
     6001 tacgcgatgt gggcttcggt cgcggcgttc atcgcggcga gcgaggtcgc ccgagggact
     6061 tcctcttcaa cggccccgct catcaccttc ctcgtcgtcg gcattggagc ccctgggtgc
     6121 tggctcggcg gcgttctggc cgatcgctgg ggacgcgtga agaccacatc actggcgatg
     6181 ctcatctccg gggcctgctc gctcgtggtc ggactcgttt tcggcgcgtc actctggttg
     6241 ctcgtacccg tactcctcat ctggggcgtc tcggtcgtcg ccgactcggc gcagttctcg
     6301 accgccatca gtgagctcgc tcctcccgag tacgtcggca ccgccctcac gttgcagacg
     6361 tcgctcggat tccttcttac ttgcgtgacg attctctcgc ttcccgtcct tgcggggtac
     6421 atcggctggc gctggtccat gacggcgctg gcggtcggcc cggcgctcgg catctgggcc
     6481 atgcaggcgc tcgggcgccg gcccgaagcc cgccagctcg cggggggcct cggctgaccg
     6541 cttcacacca cgaccccgag ggcggcttcc gcaacccgga catcccgcct ctgaatttcc
     6601 gcctcggcgc tctgctcaag tggaaattga tcgaccggcc gaagcaggtt ccgctcgcgg
     6661 ggccggttcc ccgaaaggat ccgtcgttcg tcgttccgcg cgcggctccc gaaacgcttt
     6721 cggtcacgtg gatcggtcac tccagtttct tgatccagct cggcggcctc aacctcctca
     6781 cggaccccgt ctggagcgac cgggcctcac cggtgcagtg ggccggcccg aaacgcgtct
     6841 ccccacctgg ctttccgatc gaccagcttc cccggctcga cgtggtgatc ctgtcccaca
     6901 atcactacga ccatctcgac gaccggacgg tccgccacct cgttcgcgct caccccgacg
     6961 cccgctgggt cacccccatc ggagtcggcg ctttcatcag agagcgcggc gcccggaacg
     7021 tctcggagca cgactggtgg gatgaggtgg acgtcctggg gctcaccctc gcttgcacgc
     7081 cggctcagca cttctcgggc aggggattca cggaccggaa ccagtcgctc tggtgtggct
     7141 gggcgctcaa ggcggggtcg acacgcgtgt acttctgcgg cgactccggt ttccaccctc
     7201 gcttcgtgga aatcggccgt cgtcttggcc ccttcgatgt ggcgctgatg ccgatcgggg
     7261 cgtacgagcc gcgctggttc atgagcccgg tgcacatgga tccgaaggaa gccgtgatcg
     7321 cgttccggga cctgcatcgc gcgcacgact cggaaaagcg gggagtcttc gtccccatgc
     7381 actggggcac gttccggctt accgatgagc cggtagaaga gcctcccctc ctgaccgccg
     7441 agcactggcg gaaggcgggc ctgcctgacg tgaacctctg gcggctggcg ttgggagaaa
     7501 cgcggacccg ttagtcgccg accggtacga cgaaacggac ggtgacggtc tccgccgttt
     7561 gcggctccac tacacagaag cactcccgcc cgatgattcg cttgacgtcc agtacttcgg
     7621 tttcggcgat tcccgtcaca tcgcgataga tgatctcgat ggtccgggtg tcgatttgcc
     7681 ggaacttgac cggccggtca cgcagacgat gatgggggcc gatcaggcgc aagccgtcct
     7741 cgagtcgccg ccgcagggac tccgtatcga cgatcatgat gtggctttcg cgttgcggcg
     7801 cataccggca aggccgggtc gcgaaaacgg cgtgtcatgg taccggcgcc tgaactcgtc
     7861 ctcatccaga tcggcgaacg tcttcggcgc gggcgcgacg agctccggac gaggagccag
     7921 ggcgctgtcg gtggtcaccg gtgcaaagcg ctcgttccac ggacatacgt cctggcagac
     7981 gtcgcatccg aagagccagt cgcccgtcat ccgcgcttcc agatccgtga acggcccgcg
     8041 ccgctcgatg gtgaggtagg agatgcagcg gcgcgcgtcg agcgtgcgcg gcgcggcgaa
     8101 tgcgccggtg gggcaggcgt cgagacagcg ggtacaggtc ccgcagcggt cgagctcaaa
     8161 gggaaggtcg ggctccagcg ccgcatcggt gagcacgacg ccgatgaacg tgaaggaacc
     8221 gagctccgga ttgatcagca tggaattctt gccgatccac ccaagccccg cacgctgggc
     8281 cagctcccgc tccgggaccg gaccggcgtc cacgtacgct cgtgatgtgg aacctggcac
     8341 cagccgtgcg atctcggtgg cgaggatctc gagccgctgt cccaggaccg cgtgatagtc
     8401 cgtggaccag gcgtattggg cgacgcgagg ccgggcgctc gacgcgggtc cgtggtagta
     8461 gttggtcagg gtgacgacgg cgcagcgggc gttcggaagg atcttctgcg gagctttgcg
     8521 cctggcggcc tggcgattca ggtacgtcat cgtgccgcca taccccgccg cgagccattg
     8581 gtcgatgaag tccgcatgtg ccgacggctc gagggtgcag acaccgactg ccgagaaccc
     8641 gaggtcaatc gcccgtgcct ttacgtcttc gctcgacgtc tttgctcgac gctcttgccc
     8701 gacgtttttc gcggtcacag ccccatcgtc tcccgccgcc atcttgcaaa ccggatcacg
     8761 tcctcgacgg gcggaattgc gccttcttcg ccgtacgccg tgaacattct ccatctcatg
     8821 tattcgcggg gtggcaacgg caggaagggg aagcgtcgga accagtcgcg ggcgcggaaa
     8881 ctccacgcga gtcggaggag gtccagggcc agccgggggg agagcacggc acgcccgctc
     8941 aggcggagat agaacgcggg ccaactcgcg ggagcctgca tgcgcctcgt atatttgccg
     9001 gtctatgacg ctcgccagcg gtcgcgccgc gcggcccgac ccctcgctca tcccgtccct
     9061 ggcgcgctac cgcagcgaat ttccggtatt tgcggacacc atctatctga acacctgctc
     9121 gctcggcgcg ctgggagctc gttcccggcg gaaagttacc gaattcctgg acggctggga
     9181 aaaacgtgga gcatccgcct ggtatgacgt ctggtgggag gctctccagg agctgcggac
     9241 ccggtacgcc cgcctgatcg gtgcgcaggg gagtgaaatc gcgctcgctc ccaacatctc
     9301 gacggcggtg ggcaccgtcg cgtccgccct cgactaccgg gcccgcccca aggtggtcgt
     9361 cacctccctc gacttcccga cggtcgcgta ccagtggctg gcccggcgcc ctgagggagt
     9421 cgaggtcgtg gtggtggaga gcccggacgg tgtcaccgtc ccggtggaag cgatcgcccg
     9481 ggccgtcgac gcgcggacgg cgctcgtcgc gacttcccac gtgttcttca ccaccggcgc
     9541 catccaggac attcgcgcgg tcgcgcgcgc ggcgcacgcc catggggcac tctgcctgat
     9601 cgacgcgtat caatccgtcg ggcagctccc ggtcgatgtc catgacgccg acgtcgattt
     9661 tctcgtcgcc gggggactga agtggcttct cggcggacca ggcgtcgtct tcctctatgt
     9721 acgggacgcc ctggctcgat cgctcgagcc caccctcacc gggtggttcg gccaccggga
     9781 ccagttcgcc ttcgaccccc gtgggctcac tcgccatgac gatgcacggc gctttgagct
     9841 cggcaccccc gcgctcgccg ccgtgcatgc gcagctcggc ggactcgagt acatcgagga
     9901 gatcggcgtg cctgtgattc gagaggcgtc gatggggctg gccgaagacc tgatcgcgcg
     9961 cgctcgggac cgggggtgga aaccacgggt ggcggcgtca cccaccgacc ggtccgccat
    10021 cgtcatgctc cccatgcccg accctcacgc ggcggtccgt catctggcgc aggcgcatat
    10081 catcgccgac gccaggcccg gccacgtccg cctctcacct ttcttctaca acgtgcagga
    10141 agaccatgtc gcagcaatcg agcagctcgc ccaaatcgac cactgaagac gaacgcctcc
    10201 tcgcgtcgcc caccgccaat gagcggcggt ccttcaccca ttcggaccct tggcgggtgc
    10261 tccggatcat gggggagttc gtcgaagggt tcgacaccct gcacgatgtc cgcaacgccg
    10321 tcaccatctt cggttcggcc cgcacgaaga cggacgacgc ggtctacgag caggcgatca
    10381 ccaccgccaa gctgctcgcc aaggagggct tcccggtgat caccgggggt ggccccggaa
    10441 tcatggaggc cgcgaatcgc ggggcgcagg aggggaacgg tctctccatc ggctgcaaca
    10501 tcgagctgcc gttcgagcag cacatcaacc cgtacgtgga gcgctccatc aacttccggt
    10561 acttcttcgt ccggaagacg atgttcgtga aatactcgac cgccttcgta gttttcccgg
    10621 gcgggttcgg caccttggat gagatgttcg aagccctcac cctcatccag accggcaagg
    10681 tcaagcactt cccggtcgtg ctgatgggaa cgacgtactg gggtggcctg gtcgagtggc
    10741 tgcgcgaccg tgtggccgcc gagggcaaga tcgccaccga ggacctgaac ctgattcacc
    10801 ctaccgacga cccggcggac gccgtccgct ggatcaccca ggcccgcgag cgtcgcaccc
    10861 tggcgcacgc ggcttccacc cacaacccct agcgaaaaac ccgatgactc gatcccagta
    10921 tctccgtggc cagcgcatcg atcccgcacc gatctccggc aaggagacgg ccgccgagct
    10981 gatcgacaac gcgttcctcg cctacaacgc gggccggatg cgcgagggct gccgtctctt
    11041 cgtcgagcgg atgctcgccc ccgactgcac cgtcggcatg agcctcaccg gcgcgatgac
    11101 gcccgcgggg ctcggcatgt cgaccatcat tccgctgatc gaagccgggt tcatcgactg
    11161 gatcgtgtcc accggcgcga atctctacca cgactcgcac ttcggtctgg gcctcgcgat
    11221 gcaccagggc acgccgttcg cggacgacgt cgtgctccgc gaggagggcg tggtacgcat
    11281 ctacgacatc ttcttcgact acgaagtcct gctttccacc gacgcctaca tccgcgaagt
    11341 cagcgccggc ccggagttcc agcggtcgat gagcaccgcc gagtaccact atctcctggg
    11401 gggatacatc ctggagcggg agaagaagct cggcattgcg cggaagtcgg tcctcggcgt
    11461 cgcccacgag tgcggcgtgc cgatctacac gtcgagcccc ggcgactcct ccatcggcat
    11521 gaacgtcgcc gagcaggcgc tcgcggggtc gaagctccgg tacgacgtga gcgccgacgt
    11581 gaatgaaacg gcggccatcg tctacgccgc gaagcatgga gacggcacgt ccggcgtgct
    11641 gatcgccggc ggcgggagtc ccaagaattt cgtcctccag acggagccgc agattcagga
    11701 agtcctgggg atcagcgaga aggggcacga ctacttcctg cagctcaccg acgcgcgccc
    11761 cgacacgggc gggctctccg gcgcgacgcc cgccgaggcg gtcagctggg gcaagatcga
    11821 ccccgacaag ctccccggca cggtcgtgtg ctacacggac aacacggtgg ccctgccgtt
    11881 gctcgcctcc tacgccctcg ccaagcggca acctcgccct ttgaagcggc tctatgaccg
    11941 ccgcgccgcc atgatggcgg acctgatgag ggcctatgaa aaagcccgga agtcgcgcga
    12001 cgcccgggcg gaggccgcca ctggcggacg cgcgtaaaag cgggtcgtcg gcggtcgtgg
    12061 gctccaggac gtacgcggcg tacgtcctgg gtgtcctcac actgatcaat ctcctcaact
    12121 acctcgaccg caacgtcgtc ttcgccgtct tcgagccggt caaacacgag ctccacgtca
    12181 ccgatcagga gctcggttgg ctcggttcgg cctacatcat cctcctctcg ctctgctcca
    12241 ttcccctggg cgtgctgggt gacctcaagt cgcggcgcgt cgtcatcgcc tggagtctcg
    12301 ggttctggag cctggtcacg tccgccgccg ggttcgtacg cggattcggg caactcgtgt
    12361 tctgccggtc gctcgcgggt gtgggtgaag cggggtactc gccggcctct caggcgatca
    12421 tcgccgacta ctacaagggc cgggcccggg cgttcgccat ggggatctac tcggtaggca
    12481 ccgcggtcgg cggcatcctt ggcatctggc tcggcggcgt gatcgcggcg cgatacgggt
    12541 ggcggtgggc gttcatcccg atgggaattc ccggcttgtt gctcgccgcc tccgccgccc
    12601 ggatccggga agtgaacgag cgcgccgcga caccggtgcg cgcccttctc gtgaagtggt
    12661 accgcaccgg catccgtcga gtggtgcgct acgtggtccc ggtcgcggcg ctttccgcgc
    12721 tgggcgcgat cacggcagcc gtgctcgatc tcacgttcga cctttccttc gaccggtcga
    12781 tggcggtctt cggtgtgttc gtggcggggg gggtggcgtg gacggtcgcg cgcctcgtgc
    12841 ccgtggcggt ccggcgcacg gccggcgcgg gggacgtggc ggcgtccgcg ttcgaggagt
    12901 ttctcgaagc cgccgccgtc gtacttcgga ccccgacgct gatctgggtg ttcgtcggcg
    12961 gcgcgatggt gacgtttgcg gtgaacgggt tgatcgcctg ggcggcgacg ttcatggccc
    13021 gggtgcacaa catgtcggtg cccgacttca gtgcccggtt cggcgtctgg gcgctggcgg
    13081 gcggcatttc gggtgtactg gccggcggca agatcgggga ctggctgcag atccggtggc
    13141 ccgggggccg ggtggtggcc tcgggggccg gcttcatcct gggcggcccc ctcgtcgcat
    13201 ggcttgtgtt ttccggcagt ctccggggct tcgcgccgct cctgttcggc atttacttct
    13261 tctatacctg gtacaacggg cccctcgcgg ccgtgatctt cgacgtcgtg cccgccgccg
    13321 tgcgggcctc ggtcacgggc gggttcgtgc tcttttccca cctggccgga gacgccctcg
    13381 cgccgccgct cgtgggatac ctctcggaca agacccacag cattcaaacg gcgatgttga
    13441 tccttccggt ggccggtgtg gttgggggat tcgtgatcct ggtggcgctc aagaccgtag
    13501 cgaacgacat gcgacgggtg aaacggtgag ccggcggcaa acgccgcggc ccgagctgcg
    13561 ctgcacccgg tgccgcgcgg agctcgagca cttctggtcc ttctgcccca actgcgccaa
    13621 ggcactcatc tggggtgaca cgcagcggga gaccggcgcg gagtgcgcgt attgccgctg
    13681 gatggtgtcg gacacgttct cgttctgtcc ctggtgcggc cggaacatcc acgaccggca
    13741 atcgtcgctc gagccgctca aggctccgaa gggcttcaag tatcacgcgc gctgcgattg
    13801 gggatgcggc ggcggcgtga tgtaccccat gcagtggtgt ccgtggtgcg gacgcgagca
    13861 gatctggcgc tacacgcact ttcagaacat ctgcccgcac tgcgacaagg gtgtcgacga
    13921 ctggatggac acctgtccct ggtgcgggga ggacgcgacc ggccggattc tcattccgcg
    13981 tgccctcacg cgggcgcgcc ggctcctgat cgtcagccgg atcatggatt ggaactaccg
    14041 gatactgctc aggcccggcg tttcgggtgt cgatccggac gcgccgaagg tgatcgagat
    14101 cgaccgtcgc tacgtcacgg ccaagaggcg gcgggacgag atcagctgga acatgctcac
    14161 cgggctcatt ctccatgagc tgggacactc gttcctttat catcactggt cctggaccac
    14221 cacggggcgc ttccggcggg ccttcgggga agtgcggaag gcctaccgag tggcggatga
    14281 gcactgggtg gacttcgagc ggcggaagat cgcgactacc ctgccggact acgtcagtgt
    14341 ctacgcggcg acacacccgc aggaggattt cgccgagacc ttcaggttct acgtggcgcg
    14401 gcggggcaag ctccgggagc tcttcgggga gttcgggcgg aaacgcaagg gtgtgcaact
    14461 cttcgagaaa ttcctggtcc tgcacgatta cgtgaggggc ctccgcggct ggcgctgaac
    14521 ccccgacgcg taagcatcgg cgggttatcc ccggggccac gccctggggt ttatctttct
    14581 ccccgtctct tctacccgga attgcaaagg atgcgagttc tgactcccgt cctggcgtgc
    14641 ctcctggcgg ccaggcccct gagtgcacaa acgcccgaca gcgtcaaggc gaagaccgac
    14701 acgaccaagt tgtcgttcgg cctgggcctg gtgacgacgg gcgggaacac gagcgttacg
    14761 accttcaact tcggcaacaa cttctcgtac atctcccgcc cctgggtgtt ctcggaagcg
    14821 gcgtccatgg tgtatgggtc gacgcacgat accgtcaaca acgatcagta tcatttgctg
    14881 ctccgtgtgg accacggact gcatgacggg gtgtcggcgt acgtcttcgg aacgttcgac
    14941 cgggaccggt tcgccggctt tgcgtcccgt tatcaggagg cggccggttt cgcgtggaag
    15001 gccgtcgaac acccgatgga tctattgacg ctcgaggccg gcgcgcagga gacgcagcag
    15061 cgggacctgg ggaccggggc gacggcatcc ttcacggcgg cccgcgcggc tgtggcgtac
    15121 aagcatggtt tcaacaagac cgcgtacgca caggaagccg tggaagtgct tcccgatctg
    15181 cagcacagca agaacctccg gatcaattcg gccactgacc tggtcgcacc cctgtcgacg
    15241 gtcttctcga tcaaggtcgg gtacgtgatc cggttcgaca atttgccccc tccggggttc
    15301 gagaagacgg accggatcct caccactggg gtccagatta cgctataaag gaggcgcgta
    15361 tggccaatcc ggtattgaat tcgaaaacgt tttccggctc gagagtcatg tcgggcgagg
    15421 agaccatgag catcgagggc accgtcaaca agacgggact cctgctgctc atctgtctcg
    15481 ccacggc
//
LOCUS       DASUBP010000166        12060 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1026243, whole genome shotgun sequence.
ACCESSION   DASUBP010000166 DASUBP010000000
VERSION     DASUBP010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 12060)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12060)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12060
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026243"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            138..797
                     /locus_tag="VFU75_06195"
     CDS             138..797
                     /locus_tag="VFU75_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466462.1"
                     /translation="MRSQVLLTGAVILSAVGLAAQKPMSDPDHNVAGGGTFPAGWHVR
                     TERNAPTANVKFVAMGDGFHTTQGPAAIMWRDQDTASGNYHTVATFTQTKAPMHPEAY
                     GLIVGGKNLDGDAQSYTYLIVRGTGEFSIWRRAGYANKPTAVVPWTANAAVNKAGADG
                     KDVNELAVLFKDGKASFTVNGKEVYSTDASKVDASGVIGYRVNHNLDVHLSAIGIHPL
                     H"
     gene            complement(841..2103)
                     /locus_tag="VFU75_06200"
     CDS             complement(841..2103)
                     /locus_tag="VFU75_06200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEU5466463.1"
                     /translation="MTLPRDPWPRFPSERARRAAMIALYAVVLAVALAEALRALGRME
                     GVIFSSYLDVGDAVLSGTDPYSITANTWPPFFAIEAVPLALVGRISPHLALFLWQLLG
                     LVATWGVVKLSVEFFEDGPVTFWPVSPQALALTSSAVIVPLLMTARLFDEHAQHTQIN
                     VQVLWLVLWGFHHFRRARPGLGGLSLALAASTKVVPVLLVLYLLYKRCWRETMWTVIW
                     IVILNAGLLSIAFGPADALHLWREWHAVAGNELADPTPTFMNQSLLAGVKRALTSEGG
                     LRDPVHYALLSWSPAAARIAFVVLAAAFAGMLAWLFRKHPRDLNGPRAGGELALCLGA
                     MVVVDPLAWKAHYVSLVTAYAFSWWVLRKNGAPRWQWALWWGSLVCLTGSAAAIWGRH
                     LSHVFESYDVILAGAVMLLVLTASRLDG"
     gene            complement(2100..3320)
                     /locus_tag="VFU75_06205"
     CDS             complement(2100..3320)
                     /locus_tag="VFU75_06205"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU5466464.1"
                     /translation="MLLPSSSYRTSAFIAAARQVDMDLTVASDFPSTFETAHPDALVT
                     FDFAHPARATEQARAFHARYPVSAVVGMDDDTAVLAAVIAPALGLPANPVGATEAARD
                     KHRQRELFAAAGVPIPAFSLHQLADDPDTVAGVVPYPCVLKPLRLAASRGVIRADNPG
                     QFMTARARLAAILAQPDAAACGEPARQYLVEEFIPGVEVAVEGLVEHGHLHVLALFDK
                     PDPLDGPYFEESIYVTPTRLSADVRTAIEDCAARAVRALGIERGPVHAEVRWNQRGAW
                     LIEIAARPIGGRCSAVLRFGPELMSLEELLLRRALDFPVPSLEREQTASGVMMIPVPG
                     AGTLKGVKGREEAAQVPGVVEVVITAHPGERLVPWPEGARYPGFIFARGSTGAAVEHS
                     LREAHRRLTFELER"
     gene            complement(3361..3969)
                     /locus_tag="VFU75_06210"
     CDS             complement(3361..3969)
                     /locus_tag="VFU75_06210"
                     /inference="COORDINATES: protein motif:HMM:NF020295.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HEU5466465.1"
                     /translation="MADIKITAEPTDHTRCRFVLPVPLLEGGARRFTSAAEAQDSPLI
                     ASIFGIPGLAVSEVIVSGSTVTVVKTSPSSWQQVGKSVGDAIRAALTTGTTPVSSKRV
                     EHKDAPVDDDALYDRVADLFETQVNPMVAGHGGRIELIDVQDAIVLLRMSGGCQGCGM
                     ADVTLRQGIEAMLKQEVPEVQGITDITDHASGTNPYVSASKK"
     gene            4044..5135
                     /locus_tag="VFU75_06215"
     CDS             4044..5135
                     /locus_tag="VFU75_06215"
                     /inference="COORDINATES: protein motif:HMM:NF015863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HEU5466466.1"
                     /translation="MRLRPLLLNTHLVVGLVAALPLFCIGLTGAILVFENPIDDAINA
                     KIAVVSHPPGATPLTLQALEDTLDRTYPGYRIVQASFGSDDRHSWGISATSSDGKGEA
                     DLIMDPYTGKTLGRPEQASLAVAYVHQFHTRFLAGHLGNTITGWSGVLLALLAISGLI
                     LWWPAKIVRVRAGVTGWRLAFDLHQTLGGFAWVLLLFLAGSGIVIHWNEQALNLMSKL
                     TGASAAAAAPRPAAECQGKHILDADTLLDAAQAAVPGAHATVLQISPDVTRPARVILK
                     YSEDRTPAGRTLVYVAACSAQPLQVISSRTQSAAYRWTRMWNREIHTGEVWGWPTRIL
                     AALASLTLPLMALTGPLMWWGRRGKKLVA"
     gene            complement(5137..7410)
                     /gene="metE"
                     /locus_tag="VFU75_06220"
     CDS             complement(5137..7410)
                     /gene="metE"
                     /locus_tag="VFU75_06220"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF003556.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEU5466467.1"
                     /translation="MARSHNLGFPSLGAARELKRATEGYWAGKLPAADLLETAKSLRV
                     RHWTMQRDAGIDLIPVNDFSLYDRMLDTCALVGAVPPRYALRAGSLVDLDTYFAMARG
                     SQGKGRDVVAMEMTKWFDTNYHYLVPELTTGQRFSLSSTKPVDELREARALGVNAKPV
                     LIGPVTFLLLSKTSGTGDRLGHLEALLPVYAEVLRQLKSAGAEWVQMDEPALALDRTD
                     AELKAFGRAYGYLAKEAPGINVLLATYFAGMGDNAATLHALPVAGAHLDLVRGAGDVN
                     RVLEKWPTGRILSAGIVDGRNIWRSDLEVQLSTLERIRDRVGTDALWIAPSCSLLHVP
                     QDLDLEKRLDGEIKQWLAFAKQKLAEVAVLTEAVQGGRSAVAKPLAESRVAAESRRTS
                     PRIHRESVRSRVTAVKPADERRPSSFAARRAKQVKLPAFPTTTIGSFPQTAEVRNARK
                     KLHDGTWSVSEYDKFVEGEINRTLKIQEDLGLDVLVHGEFERNDMVEYFGEQLDGFAF
                     TEHGWVQSYGSRYVKPPIIYGDVERPQAMTVRWSAYAQSRTKKPVKGMLTGPVTILQW
                     SFVRNDQPRADTAVQIALVIRDEVRDLEKAGIRVIQIDEPALREGMPLKRSEQGVYLD
                     WAVRAFRLATSGVTDATQIHTHMCYSEFNEVVRVIADMDADVISIENARSGSELLRAF
                     REFKYPNEIGPGVYDIHSPRVPSVDEIVKLLHAAREVLDERQVWVNPDCGLKTRAWPE
                     TMASLTNLVAAAKQLRG"
     gene            7445..9961
                     /locus_tag="VFU75_06225"
     CDS             7445..9961
                     /locus_tag="VFU75_06225"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5466468.1"
                     /translation="MLLPALLLLQSLCPHDSSARAALDEGWRAYRAERAQVAVEAFAS
                     ADSLCPGIRGARTGMGLALLRLGRLHEADASFRAATSDSADAEAQYGLGIVSRRLGHR
                     DAAVAAFRRALQIAPHYPDAESELLALGVTPPLPSPRRPDSPQVPARTAGDHFEIRTP
                     SGWQNFYLRGINLGAALPGKFPTEFPQAESTYTRWLSLMAAAHANVVRVYTVHPPVFY
                     RALKDWNDAHPTQALWLIHGVWAEPPPGDDYDNPAWRTDFLAEMRRVVDIIHGHAVIG
                     ARPGHSYGRYDADVSGHTLAYLIGREWEPSTIVAFDSLHPADTTFTGRFLAMRHGTPA
                     DRWMAAQCDSMLGLEWDLYHASRPIAYTNWPTLDPLHHPTETTAAEEIALRRAHGIPA
                     NPSLKEYDNDAQSLDAALVHSTPANLGGWYVSYHAYPYYPDFMDLDPEYGKARSSYGP
                     SHYFGYLLDLKQHYPGMPIIISEYGVPSSRGDAHRQPEGIDHGGHDEQAQAKYDVRLT
                     TEIREAGLAGGVLFAWIDEWFKHNWAVIDLERPAERTRLWHNAMDAEQNYGLLGEYSG
                     DTATLPVLGGDPAKWRALTALERDASVTLRVGSDAAYLYLAIEGAAPAEGGSYVVGID
                     TYGRSAGQFVLPGLKSADSTGYEFALTLRDTTGRMWVTPWYNPYLVPRPGMGPTALDA
                     FYQYGARVRRTSRTGVWDSMWVTTNRWRIARDGRTFPAGGTDRGRLRFGTQGESTLSD
                     WYVDTPANLVEVRIPWGLLNVTDPSSHEVLTGISARQGFETDTTDGFRFGVAVLGRAG
                     AEQDRLAPGPTYRWPGWEMPVSHERLKAAYFAIRDLWSKW"
     gene            9958..11421
                     /locus_tag="VFU75_06230"
     CDS             9958..11421
                     /locus_tag="VFU75_06230"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5466469.1"
                     /translation="MKRILVPVLAALIAATPVAGQILRADSAFVAGDFARARTLYEEV
                     LSADSLNERALWRLALLDSWDNQMDRSLRRFARARQLDPANADLMVAQARVLSWAGHT
                     ADAVALYDSALVTDSGRVDALAGRAQSVAWAGDLDRAEGLWRSALVDHPDDPTLLVGL
                     AQTLYWKGEPGLARAYVDRARAVAPGDASARDLERILRAAVRADFGTSFTGAGDSDDE
                     SFYYQDLWLSGPVTGDARGTLTAGWRRSADGFHHNSSFGLAGLAAFPLARGFGARAGL
                     GARRLVSDLGDHTPLTAQLGLSWQPAPFAALGLGYSRSAFDETATLIDSGFTINNLDF
                     SFDLDPKPAISISGGSSATWFSDGNRRLSWIAGVLGGLGHGVSVGAFGRMMGYQTANA
                     GHGYFSPDRFTVVEARGIYNLRRGAWGLRMDGGMGGQQVFSTFPWQGEWHLTATLSHN
                     WNGGEITLQGLYTNSAGSNTVGAFRYHAITLGFHQGL"
     gene            complement(11393..11863)
                     /locus_tag="VFU75_06235"
     CDS             complement(11393..11863)
                     /locus_tag="VFU75_06235"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEU5466470.1"
                     /translation="MSQNGASGRDPALELWIVLARAFDAVGRHARAQVTHHGLADTEF
                     GILEVLYHKGPLPVCEAQRRILLQSGSTAYVVDKLERKQLVRRTPNPRDKRGTLLVLT
                     SKGRALMSKVFPEHAATLQRAMRGLSKAEMKRAAALVRKLGLTAAATTLDEIPG"
BASE COUNT         1965 a         4104 c         4014 g         1977 t
ORIGIN      
        1 catcatgcca cctgaggcac cgagcggtgt atcaggtcgc gttcacagac ttgaccgagg
       61 cggcggggct gagtcacttg gacttccaag ctaccggcgt tctccgtccc tctccagccc
      121 acactccgag gagtccgatg cgttcccaag ttctcttgac cggcgccgtc attctgtccg
      181 cggtcgggct cgccgcccaa aagccgatga gcgaccccga ccacaacgtc gcgggcggcg
      241 gcaccttccc cgccggctgg cacgtgcgca ccgagcgcaa cgcgccgacc gccaacgtga
      301 aattcgtggc gatgggggac ggcttccata cgacccaggg tcccgcggcc atcatgtggc
      361 gcgaccagga taccgcctcc ggcaattacc acaccgtcgc gacgttcacc cagaccaaag
      421 ccccgatgca ccccgaagcc tatggactga tcgtgggtgg gaagaacctc gacggggacg
      481 ctcagagcta cacgtacctc atcgtccgcg gcaccggcga attcagcatc tggcgccgcg
      541 ccggctacgc caacaagccc accgccgtcg tgccctggac cgccaacgcg gccgtcaaca
      601 aggccggcgc cgacggcaag gacgtcaacg agctggcggt gttgttcaag gacggcaagg
      661 cgagcttcac ggtgaacggc aaagaggtct acagcaccga tgcgtcgaag gtcgacgcct
      721 caggcgtgat cgggtaccgc gtgaatcaca acctcgacgt gcatctctcg gcgatcggga
      781 ttcatcccct gcactaacta gacggttaga cggctggacg gctagcgcac tactagccgt
      841 ctagccgtct aaccgactgg cagtgagaac cagcagcatg accgcgcccg cgagtatgac
      901 gtcataactt tcgaagacgt ggctcaggtg cctcccccag atcgccgccg cacttcccgt
      961 gagacagacc aggcttcccc accacagcgc ccattgccag cgcggcgcgc cgtttttgcg
     1021 gagcacccac cagctgaacg cgtaggcggt gaccagggag acgtagtgtg ccttccacgc
     1081 gagcgggtcg acgaccacca tggcaccgag gcaaagggca agctcgccgc cggcccgggg
     1141 gccgttgaga tcgcgggggt gcttccggaa cagccaggcc agcattccgg cgaatgccgc
     1201 cgcgagcacc acgaacgcga tccgcgcggc cgccggtgac cagctcaaca gggcatagtg
     1261 caccgggtcc cggagcccgc cttccgaagt gagggctcgc ttcacgccgg cgaggagtga
     1321 ctgattcatg aatgtggggg tggggtcggc gagctcgttg ccggcgacgg catgccactc
     1381 cctccacaga tgcagggcgt ccgcggggcc aaaggcgatg gaaaggagcc cggcattcag
     1441 aatcacgatc cagatcaccg tccacatcgt ctcccgccag cacctcttgt agaggagata
     1501 cagcacgagc aggaccggaa cgaccttggt gctggcggcc agcgcgaggg acaggccacc
     1561 cagtccgggg cgggcccggc ggaaatgatg gaagccccag agcaccagcc aaagcacctg
     1621 cacgttgatc tgcgtgtgct gcgcgtgctc gtcgaacagg cgcgcggtca tgagcagggg
     1681 cacgatcacg gccgacgacg tgagcgccaa cgcctgcggg ctcaccggcc agaaggtgac
     1741 cggtccatcc tcgaagaact ccaccgacag cttcaccaca ccccaggtgg cgaccaggcc
     1801 gaggagctgc cacaggaaca gcgcgaggtg tggcgagatc cggcccacca gcgccagcgg
     1861 gaccgcctcg atcgcgaaga agggaggcca cgtattggcc gtgatggagt atggatcggt
     1921 gccggacagc accgcatcac cgacatccag gtaggatgaa aaaatcacgc cctccattcg
     1981 cccgagggcg cggagggctt cggccagcgc gacagccagc accacggcgt acagcgcgat
     2041 catcgcggcc cgccgtgccc gttccgacgg gaagcgaggc cacggatccc ggggcagcgt
     2101 caccgctcga gctcgaacgt caggcgccga tgtgcttcac ggagagagtg ctcgaccgcc
     2161 gcgccggtgg agccgcgggc gaagatgaag cccgggtagc gcgccccctc cggccacgga
     2221 accagccgct cgcccgggtg ggccgtgatc acgacctcta ccacgccggg gacctgggcc
     2281 gcctcttccc gccccttcac gcccttcagc gtgcccgcac ccggtaccgg gatcatcatc
     2341 actccggacg cggtctgctc ccgctccagc gaagggaccg ggaagtcgag ggcacgtcgc
     2401 agcagcagct cctcgagcga catcaactcc ggcccgaacc ggagcaccgc gctgcagcgc
     2461 cctccgatgg gccgcgccgc gatttcgatc agccacgccc cccgctggtt ccaccgcacc
     2521 tcggcgtgaa cggggccgcg ctcgatgccg agggcccgca ccgcacgcgc ggcgcagtct
     2581 tcgatggcgg tccgcacgtc cgcggaaagc ctggtgggcg tcacataaat cgactcttcg
     2641 aaatacggcc catccagcgg gtcgggtttg tcgaacaggg cgagcacgtg aaggtgcccg
     2701 tgctcgacca gtccctccac cgcgacttcg acgccgggga tgaactcttc caccaggtat
     2761 tggcgagccg gctccccgca ggctgcggca tccggctggg ccaggatcgc cgcgagcctc
     2821 gcccgcgccg tcatgaattg cccgggattg tcggcccgga tcaccccgcg agatgcggcg
     2881 agccggaggg gtttgaggac gcacggatac ggcacaacgc ctgcgacggt gtcgggatca
     2941 tcggcgagct ggtggagact gaaagccggg atcggaacgc ccgcggccgc gaacagctcc
     3001 cgctggcggt gcttgtcacg cgcggcctcg gttgctccca ccggattcgc cggcagtccc
     3061 agcgcgggcg cgatcacggc ggcgagcaca gccgtgtcat catccatgcc gaccaccgcg
     3121 ctcaccgggt aacgcgcgtg aaacgcgcgg gcctgctcgg tggcgcgcgc gggatgggcg
     3181 aagtcgaagg tgaccagcgc gtcggggtgc gcggtctcga acgtactggg aaaatctgat
     3241 gcgaccgtca gatccatgtc gacctggcgg gcggccgcga tgaaagcgct ggtgcggtac
     3301 gagctcgacg gcagcaacaa caggagtcgc ggtcggccgt cgcggccgtc ctgcggggag
     3361 ctacttcttg gaggcagaga cgtaggggtt ggtgcccgac gcgtggtcgg tgatgtccgt
     3421 aattccctgc acctcgggaa cctcctgctt gagcatcgcc tcgatgccct gacggagcgt
     3481 cacgtcggcc atcccgcacc cctggcaccc accggacatc cggaggagca cgatggcatc
     3541 ctgcacgtcg atcagctcga tgcggccgcc atgccccgcg accatcggat tgacctgcgt
     3601 ctcgaagaga tcggcaaccc ggtcgtagag cgcatcgtca tcgaccggag cgtccttgtg
     3661 ctccacccgt ttggacgaca ccggcgtcgt gccggtcgtc agcgccgcgc ggatggcgtc
     3721 gccgacggac ttgcccacct gttgccagct ggacgggctg gtcttcacca ccgtgacggt
     3781 actgcccgaa acgatcactt cgctcaccgc gagaccggga atcccgaaga tgctggcaat
     3841 caggggggaa tcctgggcct cggcggcgga cgtaaagcgt cgcgccccgc cctcgagcag
     3901 ggggacaggc agcacgaacc ggcaacgcgt atggtccgtg ggctcggcgg tgatcttgat
     3961 atcggccatc tggggcgaat tatcgccgag ggctccggac gggtcaaccg cgacaattcc
     4021 ccgcccaggc gcatctttat gacatgcgcc tccggccatt gctgctcaac acccatctcg
     4081 tcgtcgggct cgtagccgcc ctccccctgt tctgcatcgg cctgacgggc gccattctgg
     4141 tcttcgagaa cccgatcgac gatgcgatca acgcgaagat cgccgtcgtg tcacacccgc
     4201 ccggtgccac tcccctgact ctgcaggcgc tggaagacac gctcgaccgc acgtatcccg
     4261 gatatcggat cgtccaagcg tcgttcggat ccgacgaccg gcactcgtgg ggcatcagcg
     4321 cgacgtcctc ggacggcaag ggcgaggcag atctgatcat ggatccgtac acggggaaga
     4381 cgctcggccg ccccgagcag gcaagcctgg cggtggccta cgtgcaccag ttccataccc
     4441 ggttccttgc ggggcacctg gggaacacga tcacggggtg gagcggggtc ttgctcgcgc
     4501 tgctggccat ttccggattg attctctggt ggcccgcgaa aatcgttcga gtccgggccg
     4561 gcgtcacggg ttggcggctg gctttcgatc tccatcagac gctgggcggg ttcgcgtggg
     4621 tgttgctgct cttcctggca ggatcaggga tcgtcatcca ctggaacgag caagccttga
     4681 atctcatgag caagctcacc ggagcttccg ccgccgccgc cgccccgcgc ccggccgcgg
     4741 agtgtcaggg caagcacatt ctcgacgctg acaccctgct cgacgccgcc caggctgccg
     4801 tgcctggcgc ccacgcgacc gtgctgcaga tctcgcccga cgtcacccgg ccggcgcggg
     4861 tcattctcaa gtattcggaa gatcggacgc ccgcggggcg cacgttggtg tacgtcgcgg
     4921 cgtgctcggc tcagccatta caggtgattt cgtcccgaac ccagtccgcg gcgtaccggt
     4981 ggacccggat gtggaatcgg gagattcaca ccggggaagt gtggggctgg ccgacgcgca
     5041 tcctcgccgc tctcgcgagc ctcacgctcc ccttgatggc gctcaccggt cccctgatgt
     5101 ggtggggccg gcgggggaag aagctggtcg cgtaactcaa ccccggagct gtttcgccgc
     5161 cgcgacgagg ttggtgagcg aagccatcgt ctcgggccag gcccgggtct tcaggccgca
     5221 gtccggattc acccagacct gccgttcgtc cagcacctcg cgcgccgcgt ggagcagctt
     5281 gacgatctcg tcgaccgacg ggacgcgggg cgaatggatg tcgtagacgc cgggaccgat
     5341 ctcgttcgga tacttgaact cgcggaaggc gcgcaggagc tcggatccgg atcgggcgtt
     5401 ctcgatcgaa atgacgtccg cgtccatgtc ggcgatcacg cgtaccactt cgttgaactc
     5461 gctgtagcac atgtgcgtgt gaatctgggt ggcgtcggtc acgccgctgg tggcgagccg
     5521 gaacgcccgc accgcccagt cgagatacac gccctgctcc gaccgcttga ggggcatccc
     5581 ctcgcgaagt gccggctcgt cgatctggat gacccgaatt cccgccttct ccaagtcccg
     5641 cacttcgtcc cggatcacca gcgcgatctg taccgccgtg tccgcgcggg gctgatcgtt
     5701 ccggacgaag gaccactgca gaatggtcac cggcccggtc aacatgccct tcacgggctt
     5761 cttcgtccgg gactgggcgt acgcgctcca ccgcaccgtc atggcctggg gacgttcgac
     5821 gtctccataa atgatcggcg gcttcacgta gcgcgacccg taggactgca cccagccgtg
     5881 ctcggtgaac gcgaacccgt cgagctgctc gccgaagtac tccaccatgt cgttccgctc
     5941 gaactcaccg tgcaccagca cgtcgaggcc cagatcctcc tggatcttga gcgtccggtt
     6001 gatctcgcct tcgacgaact tgtcgtactc ggacacgctc cacgtcccgt catgcagctt
     6061 cttccgcgcg ttccgcacct cggcggtctg cgggaacgac ccgatggtcg tggtggggaa
     6121 cgctgggagc ttgacctgct tcgcccgccg cgccgcgaac gaggagggcc ggcgctcgtc
     6181 ggcgggcttc accgccgtta cgcgggaccg caccgactcg cgatgaatcc ggggcgacgt
     6241 ccggcgggac tccgccgcca cccggctttc cgccaacggc ttcgcgacgg cgctccgccc
     6301 gccctgcacg gcctcggtga gcacggccac ctccgcgagc ttctgcttcg cgaacgcgag
     6361 ccactgcttg atctccccat ccagccgctt ctcgagatcc aggtcctgcg ggacgtgcag
     6421 cagggagcac gagggcgcga tccacagtgc gtcggtcccc acgcggtccc ggatgcgctc
     6481 gagggtcgag agctgcacct cgaggtccga gcgccagatg ttccttccgt cgacgatgcc
     6541 cgccgacaga atccggcccg ttggccattt ctccagcacc cggttcacgt caccggctcc
     6601 gcgcaccaga tcgaggtgcg cccccgccac cggcaaagcg tgcagggtcg cggcgttgtc
     6661 acccattccg gcgaagtagg tcgccaggag gacgttgatg ccgggcgcct ccttcgcgag
     6721 gtagccgtac gcccgcccga aggccttgag ctccgcgtcg gtgcgatcca gcgcgagcgc
     6781 cggctcgtcc atctggaccc actcggcgcc cgcgctcttc agctggcgca ggacctcggc
     6841 gtagacgggc aggagcgcct cgagatggcc gaggcgatcc ccggtcccgg acgttttcga
     6901 cagcagcagg aaggtcaccg gtccaatgag caccggcttg gcgttgacgc cgagggcgcg
     6961 ggcttctcgc agctcgtcga ccggcttggt ggaggacagg gagaaccgct gacccgtcgt
     7021 cagctcgggg acgaggtagt gatagttcgt gtcgaaccac ttcgtcattt ccatcgccac
     7081 gacgtcgcgg cctttgccct ggctgccgcg cgccatcgcg aagtacgtat cgagatccac
     7141 cagcgacccg gcgcggagcg cataccgggg cggcaccgcg cccaccaggg cgcaggtgtc
     7201 gagcatgcgg tcgtacaggg agaagtcgtt gacggggatc aggtcgatgc cggcgtcgcg
     7261 ctgcatcgtc cagtgccgga cgcggaggga cttcgccgtc tcgagcaggt ccgcggcggg
     7321 aagttttccc gcccaatacc cctcggtggc gcgcttcagt tcgcgcgccg cgccgaggga
     7381 aggaaagccg aggttgtggg agcgtgccat agggtgcgga atctaatcgt atattccgtt
     7441 ggatatgctg ctgccggctc ttctccttct tcaatcgctt tgcccgcacg actcctcggc
     7501 gcgagccgcg ctcgacgagg gatggcgagc ataccgggcc gagcgggccc aggtagccgt
     7561 ggaggccttt gcgtcggcgg actctctgtg ccccgggatc cgtggggccc gcaccgggat
     7621 ggggctggct ctgctgcggc tggggcgtct gcacgaggcg gacgccagtt tccgcgccgc
     7681 gacctccgac tcggcggacg ccgaagcgca gtatggcctg ggaatcgtct cccggcgcct
     7741 ggggcatcgt gacgcagccg ttgcggcgtt ccggcgcgcg ctccagatcg cgccacacta
     7801 cccggacgcg gagtccgaac tgctggcgct gggtgtcacc ccgccactcc cctcccccag
     7861 gcgccctgat tcccctcaag tgccggcgcg aaccgccggt gatcacttcg agatccgcac
     7921 gccttcgggg tggcagaact tctacttgcg ggggatcaac cttggcgccg ccttgcccgg
     7981 gaaattcccg accgagtttc cgcaggcgga gtccacgtac acgcggtggc tctcgctgat
     8041 ggcggccgcg cacgcgaatg tcgtgcgcgt gtacacggtg catccgccgg tgttctatcg
     8101 cgcgctgaag gattggaacg acgcgcaccc gacccaggcc ttgtggctca ttcacggcgt
     8161 atgggcggag ccgccccccg gggacgacta cgacaaccca gcgtggcgca cggattttct
     8221 cgcggagatg cgccgggtag tggacatcat ccacggccac gcggtcatcg gggcccgccc
     8281 gggacactcg tatggccgat acgacgccga cgtgtccggc cacaccctcg catacctgat
     8341 cggccgggag tgggaaccgt ccacgatcgt ggcgttcgac tcgctgcatc cggccgacac
     8401 gaccttcacg ggccgctttc tggcgatgcg gcacggcact cccgcggacc gttggatggc
     8461 ggcgcagtgt gactcgatgc tgggactcga gtgggatttg tatcatgcgt cgcgcccgat
     8521 cgcctatacc aactggccga cgctcgatcc cctgcaccac cctacggaga cgaccgccgc
     8581 cgaggaaatc gcgctgcgcc gggcacacgg cataccggcg aacccgtctc tgaaagagta
     8641 cgacaacgac gcccagtccc tcgacgccgc gctggtccat tccacgccgg caaatctcgg
     8701 ggggtggtac gtgtcgtacc acgcatatcc gtactatccc gacttcatgg acctggaccc
     8761 ggagtacggg aaggccaggt cgtcatatgg cccgtcccac tacttcggct atctgctcga
     8821 cttgaagcag cactacccgg ggatgcccat catcatctcg gagtacggcg tcccgtcttc
     8881 ccggggagat gcgcaccggc agccagaagg aatcgatcat ggcggtcatg acgagcaggc
     8941 ccaggcgaag tacgacgtcc ggcttaccac cgagatccgc gaggcggggc ttgccggcgg
     9001 cgtgctcttc gcgtggatcg acgagtggtt caagcacaac tgggcggtga tcgacctgga
     9061 aaggcccgcc gagcggacca ggctctggca caacgcgatg gacgctgagc agaactacgg
     9121 gctgctcggg gagtactccg gggacacggc gacactcccc gtcctcggcg gcgacccggc
     9181 gaagtggcgc gcgctcacgg cgctcgaacg cgatgcgtcg gtgactctgc gcgtgggatc
     9241 ggacgccgcg tacctctatc tggccatcga gggcgccgcg ccggcggagg gtgggagtta
     9301 cgtggtgggt atcgacacgt acggccggag cgcggggcaa ttcgtgctgc cggggctcaa
     9361 gtccgccgat tcgacgggat acgaattcgc gctgacgttg agggacacca ccggacggat
     9421 gtgggtgacc ccttggtaca acccctacct cgtacccagg ccgggaatgg ggcccacggc
     9481 cctggatgcc ttctatcagt atggcgcccg ggtgaggcgc acgagccgga cgggcgtttg
     9541 ggattcgatg tgggtgacga ccaaccgctg gcgcatcgcc cgggacggac ggaccttccc
     9601 cgcgggcggc acggatcggg ggcggctgcg gttcggcacc caaggggaat caaccctgtc
     9661 cgattggtac gtggacaccc ccgcgaacct ggtcgaggtc cggatcccct gggggctgtt
     9721 gaacgtgacc gacccttcat cccacgaagt gttgaccggc atttcggcgc ggcagggttt
     9781 cgaaaccgat acgacggacg gcttccggtt cggcgtcgcg gtgttgggca gggcgggagc
     9841 ggagcaggac cgcttggcgc cgggaccgac ctaccggtgg cccggatggg agatgccggt
     9901 atctcacgag cggctcaagg ccgcatactt cgccatccgc gatctgtgga gcaaatggtg
     9961 aaacggattc tcgtgccggt cctcgccgct ctcatcgccg ccacgcccgt ggcggggcag
    10021 atcctgcgcg cggattccgc attcgtcgcc ggggatttcg cgcgggccag aaccctgtac
    10081 gaggaagtac tctcggcgga ctcgctcaac gagcgggcgc tgtggcgcct ggctttgctc
    10141 gacagctggg acaaccagat ggaccggtcc ctgcggcgtt tcgcccgggc ccgccagctc
    10201 gacccggcca acgccgactt gatggtggcg caggcgcgcg tgttgtcctg ggccgggcac
    10261 accgcggacg cggtcgccct gtatgactcc gcgctcgtca ctgactcggg ccgggtggac
    10321 gcgctggcgg gtcgggcgca gagcgtggca tgggccggtg acctggatcg cgccgaagga
    10381 ctctggcgct cggcgctggt tgaccatccg gacgacccga ccctgctcgt aggtctcgcg
    10441 cagaccctct attggaaggg agagccggga ctggcccgtg cgtatgtgga tcgggcccgg
    10501 gcggtggcac ctggtgacgc gtcagcccgc gatctcgaga ggattctgcg tgcggcggtg
    10561 cgcgccgatt tcggaacgtc gtttacgggt gccggcgact cggacgacga gtccttctac
    10621 tatcaggacc tctggctctc aggcccggtg acgggtgacg cccgcggcac cctcaccgcg
    10681 ggctggcgcc gctccgcgga cggcttccac cataattcga gcttcggctt ggccggcctg
    10741 gcggcgttcc ctctcgcccg tggcttcggc gcgcgcgccg gactgggcgc ccggcggctg
    10801 gtgtcggatt tgggagatca cactcccctg accgctcaac tcggtctctc ctggcagccc
    10861 gctccgttcg ccgccctcgg cctggggtat tcccggtcgg cgttcgacga gaccgcgacg
    10921 ctgatcgaca gcgggttcac catcaacaat ctcgacttct cgttcgatct ggaccccaag
    10981 ccggcgattt ccatctcggg cggatcgagt gccacgtggt tttcagacgg caaccggcgg
    11041 ctgtcgtgga tcgcgggtgt gttgggggga ttggggcatg gggtgtcggt cggcgcgttc
    11101 ggacgcatga tgggatacca gacggcgaac gccgggcatg gctacttctc cccggaccgg
    11161 tttacggtgg tcgaggcgcg cggcatttac aatttgcgtc ggggggcttg gggcctgcgg
    11221 atggacggag gcatgggcgg tcagcaggtc ttcagcacgt tcccgtggca gggcgagtgg
    11281 cacctcaccg ccacgctgtc acacaactgg aatggcggcg agatcacgct gcagggactc
    11341 tacacgaact cggcggggtc gaacacggtg ggcgccttcc gttatcacgc catcaccctg
    11401 ggatttcatc aagggttgta gccgccgcgg tcaggccgag tttccggacc agggccgccg
    11461 cgcgtttcat ctccgctttc gacaatccgc gcatcgcccg ctgaagcgtc gccgcgtgtt
    11521 ccgggaagac cttcgacatg agcgcgcgcc cttttgacgt cagcaccagg agagtgcccc
    11581 gcttgtcccg cggattcggg gtccgccgga ccagctgttt cctctccagc ttgtcgacta
    11641 cgtacgccgt actcccgctc tggagcagga tccgccgctg ggcctcgcag acgggcaggg
    11701 gtcccttgtg atacagcact tccaggatgc cgaactccgt gtccgccagc ccgtggtggg
    11761 tgacctgggc acgggcgtgc cgcccgacgg cgtcgaacgc gcgggccagc acgatccaca
    11821 gttccagggc cggatcccgg cccgatgctc cattctgcga catatccccc ctgctctggg
    11881 aacctggaag cgggcccggc ggttcccagt gaggcaactc gaaactagct cgaattcgag
    11941 caaactcaac tggaggccgt cggtatgtgg accaatctcg cgctgctctt cctcaggctc
    12001 gtcgtcggag ccctgttcgt cggtcacggg acccagaagg cgttcggctg gttcggcgga
//
LOCUS       DASUBP010000167         3399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_277270, whole genome shotgun sequence.
ACCESSION   DASUBP010000167 DASUBP010000000
VERSION     DASUBP010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3399
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277270"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..205)
                     /locus_tag="VFU75_06240"
     CDS             complement(<1..205)
                     /locus_tag="VFU75_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466471.1"
                     /translation="MAAALTVGEVGEGEYGRWEKFIAAAPGGSVYSTPGYLDALCTAA
                     GGRFRLLAARQGEEIAGGVALYER"
     gene            complement(206..1393)
                     /locus_tag="VFU75_06245"
     CDS             complement(206..1393)
                     /locus_tag="VFU75_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466472.1"
                     /translation="MTHKVPVLVFGTGLTVLGAIRLLHRSGTPFWVVGDGGELAGHSR
                     WFRTAPPSTSGADPAKDLARYLSGLPFERAVLLPCSDAWVQRVAAVAARVAPRFVASV
                     SAPETLERLVDKWHLAEILQELGLPHPRTVAIAATQDPASVPEEVLKGAFMKPRDSLN
                     FFARFGVKAFRISSRAELRECLGRLAGTGLEVQLQEYIPGPPGNHYFVDGFRDRNGRV
                     TGTQARRRMRMYPPDFGNSTSFESIPPSDVQPAVDAMTRLLAHLKFRGVFSAEFKRDD
                     RDGVFRLIEVNVRPWWYVEFAGRSGMDVTAMVVRDALGEPVEPARPSRAGRRCVYPYY
                     DYFACREGLRDGTLTLGGWLRSWAGAEQPVFRWSDPWPALSASAHILWGKVRRLAGGG
                     AHT"
     gene            complement(1390..2418)
                     /locus_tag="VFU75_06250"
     CDS             complement(1390..2418)
                     /locus_tag="VFU75_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemAB family XrtA/PEP-CTERM system-associated
                     protein"
                     /protein_id="HEU5466473.1"
                     /translation="MTGSAGIAVAPFTGASQDWDALVERSPGGTHFHRWGWKQVMERA
                     FGHECTFLGATEQGTLRGVLPLTSVRSVLFGRFLVSMPFVNYGGPLGDQPAVQALARE
                     AVARADRTGVKLLELRSRVELPLDLPVSHRKITVVLDLPTRDPKALWESLPAKVRSQI
                     RRPQKEGVTVAFGPDQLDGFYRVFARHMRDLGTPVQSRRLFEEIRAVFPNDVWFGCAW
                     LKGEPIAGGCGFRWGNEFEITWASALRAHQTIAPNMALYWGFLERCVEQGVTLFNFGR
                     CTPDSGTHRFKRQWGSRDEPLWWYAHGAATTPSPDDGAYSWGPRLWKFLPVPVANVIG
                     PRIVKFIP"
     gene            complement(2415..3263)
                     /locus_tag="VFU75_06255"
     CDS             complement(2415..3263)
                     /locus_tag="VFU75_06255"
                     /inference="COORDINATES: protein motif:HMM:TIGR03006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XrtA system polysaccharide deacetylase"
                     /protein_id="HEU5466474.1"
                     /translation="MTTHYFSVDVEEYFQVSAFEGSVPRASWEHLPSRVEASVYGILD
                     LLARHGARGTFFTLGWVAERHPQMIQRIAAAGHEVASHGWDHVRVTHQTPDEFRASVK
                     RSKACLEQLTGTQVLGFRAPSFSIVPGREWALDILVEEGYRYDSSLFPIRRPGGYGYA
                     SAGRDPFVLERAAGKLDELPPATLRRLGVNLPAGGGAYFRILPYALVQNAFRYFEARG
                     VPGTFYMHPWEIDPDQPRQQAKLSSRLRHYTGLSRTLPRLERLLSEFRFTAIADRFTR
                     AALAAS"
BASE COUNT          597 a         1178 c         1152 g          472 t
ORIGIN      
        1 cacgctcgta gagtgccaca cccccggcga tctcctcacc ttgccgggcc gcgagcagtc
       61 ggaagcgccc gccggccgcc gtacagagcg cgtcgagata tccgggcgtg ctgtagacgc
      121 tgccgcccgg agcggccgcg atgaacttct cccaacgccc gtactcgcct tcgccgacct
      181 ctccgacggt gagtgcggca gccacctagg tgtgggcccc gccacccgcc aggcgacgca
      241 cctttcccca caggatatgc gccgaggccg acagtgccgg ccagggatct gaccagcgga
      301 acacgggctg ttcggcgcct gcccacgacc ggagccaccc acccagcgtg agcgtgccat
      361 cccgcaggcc ctcacggcag gcgaagtagt cgtagtaggg atacacacac cggcgtcccg
      421 cccgcgacgg ccgcgcgggt tccacgggct cccccagcgc atcccgcacg accatggcgg
      481 tgacgtccat gccgctgcgc cccgcgaatt ccacatacca ccagggtcgc acgttcacct
      541 cgatgagccg gaagacgccg tcgcgatcgt cgcgcttgaa ttcggcactg aacactcccc
      601 ggaacttgag atgagcgagc aagcgggtca tcgcgtccac cgcgggctgg acgtccgagg
      661 gcgggatcga ctcgaagctc gtgctgttgc cgaaatccgg gggatacatc cgcatgcggc
      721 gacgagcctg cgtgcccgtc acccggccgt tacgatccct gaatccgtcg acgaagtagt
      781 ggttgcccgg cgggccgggg atgtattcct gaagttgcac ttcgagacct gttcccgcga
      841 gcctgccgag acactcgcgg agctcggcgc gcgaggagat gcggaacgcc ttgactccga
      901 accgggcgaa gaagttcaag gagtcccggg gcttcatgaa cgcgcccttc aggacttcct
      961 ccggcaccga ggccggatcc tgtgtggccg cgatcgcgac ggtgcgcggg tgcgggagcc
     1021 cgagctcctg aaggatttcc gcgagatgcc atttgtcgac gagccgctcg agggtctccg
     1081 gagcggagac gctggcgacg aaccgcggcg cgactcgagc cgcgacggcg gcgacccgct
     1141 gcacccaggc atccgaacac ggcagcagaa cggcccgctc gaaggggagc cccgacaggt
     1201 atctcgcgag atccttcgcc ggatcggcgc ccgacgtgga cgggggcgcc gtgcgaaacc
     1261 agcgtgaatg tcccgccagc tcgccgccgt cccccaccac ccagaacggc gtgcccgaac
     1321 ggtgcagcaa cctgatggcg ccgagcaccg tcagaccggt accgaacacg agcacaggga
     1381 ccttgtgcgt cacgggatga acttgacgat gcggggaccg atgacgttgg cgacggggac
     1441 gggcaggaac ttccagagcc gtggtcccca gctgtacgcg ccatcgtcgg gcgatggagt
     1501 cgtcgcggcg ccatgcgcgt accaccagag cggttcgtcg cgtgaccccc attgccgctt
     1561 gaagcggtga gtgcccgagt ccggggtaca acgcccgaag ttgaagagcg tcaccccctg
     1621 ctccacacag cgctcgagga agccccagta aagcgccatg ttcggcgcga tggtctggtg
     1681 cgcgcgcaat gccgacgccc aggtgatctc gaactcattc ccccaccgga agccgcaccc
     1741 gcccgcgatc ggctctccct tgagccaggc gcacccgaac cagacgtcgt tcggaaacac
     1801 cgcccggatc tcctcgaaca gccgccgcga ctgcaccggt gtgccgaggt cacgcatgtg
     1861 acgcgcgaac acgcggtaga acccgtcgag ctggtcggga ccgaaagcga ccgtcacgcc
     1921 ttccttctgc gggcggcgga tctgggaacg taccttggcg ggaaggcttt cccagagggc
     1981 ttttgggtca cgggtcggga gatcgaggac gacggtgatc ttccggtgcg acaccggcag
     2041 gtcgagggga agctcgaccc ggctgcgaag ctcgagcagc ttcacgcccg tgcgatcggc
     2101 gcgcgcgacc gcctcgcgag ccagggcctg caccgccggc tggtccccca gggggccgcc
     2161 gtagttgacg aagggcatgg acacgagaaa ccggccgaag agaacgctcc ggacgctcgt
     2221 gaggggcagg acgccgcgca gggtgccctg ctccgtggcg ccgaggaacg tgcactcgtg
     2281 gccgaaggcc cgctccatca cctgcttcca gccccagcgg tggaagtgcg tacctcccgg
     2341 gctgcgttcc accagggcat cccaatcctg gctggctccg gtgaacgggg cgacggcgat
     2401 accggcggat ccggtcacga cgcggcgagc gcggcgcggg taaagcggtc ggcgatggct
     2461 gtgaagcgga actcgctcaa gaggcgctcg agacgcggca gggtccggga cagacccgtg
     2521 tagtgccgga gccgggacga caacttggcc tgctggcgag gctggtcggg atcgatctcc
     2581 cagggatgca tgtagaacgt ccccggcaca cctcgcgcct cgaagtaccg gaaggcgttc
     2641 tgcaccagcg catatgggag gatccggaaa tacgcaccac cgcccgccgg caggttcacg
     2701 cccaaccgcc gcaacgtcgc gggcggcagc tcgtcgagct tcccggcggc gcgctcgagc
     2761 acgaacgggt cgcgccccgc cgacgcgtag ccgtaacccc cgggacgccg gatcggaaag
     2821 aggctcgaat cgtagcggta cccctcctcc accaggatgt cgagcgccca ctcgcgcccc
     2881 ggcacgatgg agaaggacgg tgcgcggaat ccaaggacct gcgtgccggt gagctgctcg
     2941 aggcaggcct tggaccgctt cacggacgca cggaattcgt cgggggtttg atgcgtgacg
     3001 cggacgtggt cccatccgtg gctggccacc tcgtgaccgg ccgccgcgat ccgctggatc
     3061 atctgcggat ggcgctccgc cacccacccg agggtgaaga acgtgccccg ggccccgtgg
     3121 cgggccaaga ggtcgaggat tccgtagacg gacgcctcga cgcgtgacgg gaggtgctcc
     3181 cagctggcgc gagggacgct tccctcgaag gccgagacct ggaagtactc ctcgacgtcg
     3241 acgctgaaat agtgggtggt catcagcccc aaaggtagca aaaaaagagc ccggctctct
     3301 aggccgggct ctccaggcgt ggaatccgcg gggtcaggcc gcggtcttct cagccgcctg
     3361 tttgcggcgc cgacgcagcg cgccaccacc catgccgac
//
LOCUS       DASUBP010000168         5247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_290470, whole genome shotgun sequence.
ACCESSION   DASUBP010000168 DASUBP010000000
VERSION     DASUBP010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_290470"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /gene="guaA"
                     /locus_tag="VFU75_06260"
     CDS             complement(<1..1065)
                     /gene="guaA"
                     /locus_tag="VFU75_06260"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEU5466475.1"
                     /translation="MNRILILDYGSQFTQLIARRIREAHVYCEIHPGDKGRDLAFVKA
                     FAPQGIIFSGGPASVFAKGAPSADPAILDLGIPILAICYGLQLVAYIGGGKVVASPEQ
                     EYGRADVTVKVAEGIFQGFEAGRAITVWASHSDRIEVPPPGFKVIASSANAPIAGLAH
                     ETKPVMGVLFHPEVAHTVRGGEIIHNFLFGICKCTPDWTAGHYIEQEIAKIRNTVGPA
                     RRAICGLSGGVDSAVAAALVHRAIGDRLTCIFVDHGLLRENERQQVEQTFRKHLGIDL
                     RVVDASARFLKELAGIEDPEEKRRRIGHTFIDVFEDAAKHVSGDVGFLVQGTLYPDVI
                     ESSSPKGGPSVTIKTHHNVGG"
     gene            complement(1062..2321)
                     /gene="lysA"
                     /locus_tag="VFU75_06265"
     CDS             complement(1062..2321)
                     /gene="lysA"
                     /locus_tag="VFU75_06265"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR01048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEU5466476.1"
                     /translation="MGAGILGLDPALLTAVAKRVGTPTYVYGANLIRAQYHALEDALR
                     AGGTPHRICYAIKANGSLAVLRVLKGLGAGADIVSLGELARALRAGFPPESIVFSGVG
                     KTADELDAAIKSCVGQINIESPSELDAVIAATARVGKHAGVGIRVNPDVQTNTHPYTK
                     TGDKTAKFGVPFDEVVTVARRIKVESRLTLKGLAMHIGSQITEVEPYRRGAVKLLELV
                     AAVRATGITTIDNVDVGGGLGVRYHEEKVPALKDWVDAVAPPIRAAGLTLHCEPGRFL
                     VANAGLLLTRVLYQKHAGGKDYCIVDAGMTDFVRPSHYNAYHEIIPLSDAGRGERSVN
                     VVGPICETGDFLALDRKLPPVAPGDVLAVLGTGAYGFVMSSTYNARPRPPEVLVEGDK
                     YYVARERETIEDLFRGEVLEPQTWFTS"
     gene            complement(2303..3805)
                     /gene="proS"
                     /locus_tag="VFU75_06270"
     CDS             complement(2303..3805)
                     /gene="proS"
                     /locus_tag="VFU75_06270"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEU5466477.1"
                     /translation="MAQAEKITPRAKDFSEWYNDVIMQAQLADYSPVRGCMVIRPLGY
                     RIWELMQRALDDMFKATGHQNAYFPLFIPQSFLSKEAEHVEGFAKEVAVVTHTRLRAT
                     GKTGADAVEPDPASELEEPLVVRPTSETIIYAMFAKWIQSYRDLPLLMNQWCNIVRWE
                     LRTRLFLRTTEFLWQEGHTAHATEADAEAEARQMLGVYRKFQEEWVAMPVLTGKKTES
                     EKFAGALRTYAVEALMQDNKALQAGTSHNLGQNFARAFNVQYQTAAGGLDFVWNTSWG
                     VSTRMIGGLIMTHSDDNGLVAPPRLAPIQVVIVPIWKSDEEKGQVSGVGGQVKDALAK
                     AGIRVELDLRDMKPGAKYFDWEAKGVPLRLEIGPRDIAAGQVMAARRVKSATGGKAPL
                     GLNDLVTAVPKLLEEIQHELFEAAKARREANSIRGVNKTQFLEFMNGTGGFAYGGFCG
                     SGDCEAQIKTETAATIRVLPDPEFQSPEKPRTCMWCGMPSVAEAVWAQAY"
     gene            complement(3805..5091)
                     /gene="hisS"
                     /locus_tag="VFU75_06275"
     CDS             complement(3805..5091)
                     /gene="hisS"
                     /locus_tag="VFU75_06275"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: protein motif:HMM:TIGR00442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEU5466478.1"
                     /translation="MQGKALPGFRDFYPDELALRNHIFATWRGVAARYGFEEYDGPPL
                     EPLELYTQKSGEEIVGQLYNFRDKGDRDVALRPEMTPTLARMVGARAQALKKPIRWFS
                     IPQLFRYERQQRGRLREHFQLNMDLIGEAGPLADAELIAAAIDIMRGFGLSAKDVQAR
                     ISDRRVLKALLLGNGLTEAQLPTAFAAIDKAERDPMDVLERKLGEAGIGKRETGNVFA
                     IAALRDWSAVKEALGKVRGGAEAGEPLEQSLSALKAMGLGDYATVDMTIVRGLAYYTG
                     TVFELFDTGRTLRAICGGGRYDGLLKALGGVDLPALGFGMGDVVLGELLKERDKGAGK
                     PGAVHAFLVGISGDDVPFILELAHHLRDQGIGVEYALKQGNIRKQLELAAARGAARAV
                     ILGPDERAAGVAVVRDLNAGTEVKVPLTSIRNGSFQ"
BASE COUNT          875 a         1812 c         1619 g          941 t
ORIGIN      
        1 gccgccgacg ttgtggtgcg tcttgatcgt gaccgacgga ccacccttcg gagaagacga
       61 ctcgatgacg tccggataga gggtcccttg caccaggaat ccgacgtctc ccgacacgtg
      121 cttcgccgcg tcctcgaata catcgatgaa cgtatgcccg atccgccgac gcttctcctc
      181 gggatcctcg atgccggcca gctccttgag aaaccgggcg ctggcgtcca ccacccgaag
      241 gtcgatcccg agatgcttcc ggaacgtctg ctcgacctgc tgccgttcgt tctcgcgaag
      301 caacccatgg tccacgaaga tgcaggtgag acggtcgccg atcgcgcgat ggaccagcgc
      361 cgcggccacc gccgagtcga cgccaccaga caacccgcag atcgcgcgcc tggcggggcc
      421 cacggtattc cggatcttcg cgatctcctg ctcgatgtag tgccccgccg tccagtcagg
      481 cgtgcacttg cagatgccga acaggaagtt gtggatgatt tctccgcccc ggaccgtgtg
      541 tgccacttcc ggatggaaga gcacgcccat caccggcttg gtctcgtgcg cgagccccgc
      601 gatgggcgcg ttggccgagg aggcaatgac cttgaagccg ggcggcggca cctcgatgcg
      661 gtccgaatga ctggcccaga cggtgatggc ccggcccgct tcgaatccct ggaagatccc
      721 ctcggccacc ttcaccgtga cgtcggctcg cccgtattcc tgttcgggag aggccaccac
      781 cttgccgcca ccgatgtagg ccacgagctg gagcccgtag cagatggcga gaatcggaat
      841 ccccagatcg aggatggccg gatcggcact gggcgcgccc ttggcgaaga ccgacgccgg
      901 cccacccgaa aagatgatgc cttgcggagc gaacgccttt acgaaggcga ggtcgcggcc
      961 tttgtctccc ggatggattt cacagtagac gtgggcctcg cggatccggc gggcgatgag
     1021 ttgggtgaac tgcgagccgt agtcgaggat gaggattctg ttcatgaggt aaaccatgtt
     1081 tgtggttcca gaacttcacc ccgaaacaga tcttcgatcg tttcccgctc gcgggccacg
     1141 taatacttgt ccccttccac caggacctcg ggggggcgcg gccgcgcgtt gtacgtagag
     1201 ctcatgacga agccgtacgc gccggtgccc aacaccgcca ggacgtcgcc aggcgccacc
     1261 ggcggcagct tgcggtcgag tgccaggaaa tcgccggtct cgcaaatcgg cccgacgacg
     1321 ttcaccgagc gctcccccct ccccgcatcg ctcaggggaa tgatttcgtg gtaggcgttg
     1381 tagtgactgg gacggacgaa atcggtcatg ccggcgtcca cgatacaata gtccttgcca
     1441 cccgcatgct tctgatagag cacacgggtg agcaacaatc cggcattggc gacgaggaac
     1501 cgtcccggct cgcaatggag cgtgaggccg gcggcccgga tcggcggcgc caccgcgtcc
     1561 acccaatcct tgagcgccgg caccttctcc tcgtgatatc gcacccccaa cccgccaccg
     1621 acatcgacgt tgtcgatggt cgtgatcccc gtcgcgcgca ccgccgccac cagctccagg
     1681 agcttgaccg cgccgcggcg atacggctcc acctcggtga tctgggaccc gatgtgcatc
     1741 gccagacctt tgagcgtgag cctcgactcc accttgatcc tgcgtgccac ggtcaccact
     1801 tcatcgaacg gcaccccgaa cttggcggtc ttgtcccccg tcttggtgta cggatgcgtg
     1861 ttcgtctgga cgtcggggtt tacgcggatc ccgacgccgg catgcttccc cacgcgagcc
     1921 gtcgccgcga ttacggcgtc gagctcgctg ggcgactcga tgttgatctg ccccacgcat
     1981 gacttgatcg cggcatccag ctcatccgcg gtcttcccga cacctgaaaa gacgatcgac
     2041 tcggggggga agcccgcacg gagcgcgcgc gccagctccc cgagggaaac aatgtccgct
     2101 cccgcgccga ggcccttgag gacccggagg acggcgaggg aaccgttggc cttgatggcg
     2161 tagcagatcc ggtgcggcgt tcccccggcc cggagtgcgt cctccaaggc gtggtactgc
     2221 gcccggatca ggttggcacc gtaaacgtag gtgggagtcc ccacccgttt ggcgacggcg
     2281 gtgagaagcg cgggatcgag gcctagtatg cctgcgccca caccgcctcc gccacgctcg
     2341 gcatcccgca ccacatgcag gtgcgcggct tttccggtga ctggaactcg gggtccggaa
     2401 ggacgcggat ggtggcggcc gtctcggtct tgatctgcgc ttcgcagtcg cccgagccgc
     2461 agaacccgcc gtaggcgaac ccgccggtac cgttcatgaa ctccaggaat tgcgtcttgt
     2521 tgacgccccg gatggagttc gcctcgcggc gggccttcgc ggcctcgaag agctcgtgct
     2581 ggatctcctc gagcaacttc gggaccgcgg tgaccagatc attcaagccc agaggcgcct
     2641 ttccaccggt cgcactcttc acgcgacggg cggccatcac ctgtccggcg gcgatatcgc
     2701 ggggcccgat ctcgagacgg agcggcaccc ccttcgcctc ccagtcgaaa tacttggcgc
     2761 ccggcttcat gtcccggagg tccagctcga cccgaatccc ggccttggcc agagcgtcct
     2821 ttacctgacc cccgacccct gacacctgcc ccttctcttc atcggatttc cagataggga
     2881 caatcacgac ctggatcggc gccagccgcg ggggcgccac gagcccgttg tcgtcggagt
     2941 gcgtcatgat cagcccgccg atcatgcggg tggacacgcc ccaggacgtg ttccacacga
     3001 agtcgagccc accggccgcg gtctggtact ggacattgaa ggcgcgcgcg aagttctggc
     3061 ccaggttgtg agaggttccc gcctggagcg ccttgttgtc ctgcatcagt gcttcgaccg
     3121 cgtaggtgcg cagggcgccg gcgaacttct cgctctcggt cttcttgccc gtcagcaccg
     3181 gcatggcaac ccactcttcc tggaacttcc ggtagacacc cagcatctgc cgcgcttcgg
     3241 cctccgcatc cgcttccgta gcgtgcgcgg tgtgcccttc ctgccagagg aactccgtgg
     3301 tccgcaggaa caggcgggtg cggagctccc agcggacgat gttgcaccac tgattcatga
     3361 gcaggggcag gtcgcgatag ctctggatcc acttcgcgaa catcgcgtag atgatcgttt
     3421 ccgacgtcgg acgcaccacc agcggctctt ccagctcgga tgccggatca ggctcgaccg
     3481 cgtccgctcc ggtcttcccc gtcgctctga gccgcgtgtg cgtgaccacc gcgacttcct
     3541 tcgcgaagcc ctcgacgtgc tcggcctcct tggacaggaa gctctgcgga atgaagaggg
     3601 ggaagtaggc gttctgatgc cccgtcgcct tgaacatgtc gtccagcgcg cgctgcatca
     3661 gctcccagat ccggtagccg agggggcgga tgaccatgca gccgcgcacg ggggaataat
     3721 ccgcgagctg agcctgcatg atcacgtcgt tgtaccactc gctgaaatcc ttggcgcgtg
     3781 gggtgatctt ttccgcttga gccattactg gaatgatccg tttcgaatcg aggtcagggg
     3841 cacctttact tcagtgcccg cgttgaggtc ccgcaccacg gctacaccgg cggcgcgttc
     3901 atcagggccg aggatcaccg ctcgcgcggc gccccgtgcg gccgccagct cgagttgttt
     3961 ccggatattg ccctgcttga gcgcgtactc gacaccaatc ccctggtcgc gaaggtggtg
     4021 agccagctcg aggatgaacg gtacgtcatc accggagatc cccacgagaa aggcgtgcac
     4081 ggctcccggc ttcccggcgc ctttgtctcg ttccttgagc agctcaccga gcaccacatc
     4141 gcccatcccg aatccgagtg ccgggaggtc gacacccccg agcgccttca ggagcccgtc
     4201 gtagcggcca cccccgcaga tggcccgcag ggtacgtccg gtgtcgaaca gctcgaacac
     4261 cgtcccggtg tagtaggcga gcccgcggac gatggtcatg tccacggtcg cgtagtcgcc
     4321 cagccccatc gccttgaggg cgctcaggga ttgctcgagc ggctcccccg cctcggcccc
     4381 tccccgcacc ttccccaacg cttccttgac cgcactccaa tcccgcaagg cggcgatcgc
     4441 aaacacgttt cccgtttccc gcttcccgat tcccgcctcc ccgagcttcc gctccaacac
     4501 gtccatcggg tcccgctcag ccttgtcgat ggcggcgaac gcggtcggga gctgggcctc
     4561 cgtcagtccg ttccccagaa gcagtgcctt cagcacccgc cggtccgaaa tcctggcctg
     4621 cacgtccttg gcggagagcc cgaacccccg catgatgtcg atggcggcgg cgatcagctc
     4681 ggcgtccgcc aacggccccg cctcgccgat caggtccatg ttcagctgga agtgctctcg
     4741 aagccggccc cgttgctgcc gttcgtaccg gaagagctgg gggatggaga accagcgaat
     4801 gggcttcttg agggcctgcg cgcgagcccc gaccatccgg gcgagcgtag gggtcatctc
     4861 gggccgcagc gcgacgtccc ggtccccctt gtcacggaag ttgtagagct gcccgacgat
     4921 ctcttccccg gacttctggg tgtacagctc gaggggttcc agcgggggac cgtcgtactc
     4981 ctcgaagccg taacgtgccg ctacccctcg ccaggtagcg aagatgtgat tgcggagtgc
     5041 cagttcgtca gggtagaaat cacggaagcc gggaagcgct ttcccttgca tcacaacaat
     5101 ttagccgact ggcgcaaacc cgggcaaggc ggcgagcgcg tcgccgacgg tgtgaaatga
     5161 gcccgtcacc aacgtcgtgg cggcgccttc ctgcaccatc tccagcgcgc gcgtgaagtc
     5221 cggctcgaac accaccggct gaccgat
//
LOCUS       DASUBP010000169        27822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1353643, whole genome shotgun sequence.
ACCESSION   DASUBP010000169 DASUBP010000000
VERSION     DASUBP010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 27822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27822
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1353643"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(89..1510)
                     /gene="bshC"
                     /locus_tag="VFU75_06280"
     CDS             complement(89..1510)
                     /gene="bshC"
                     /locus_tag="VFU75_06280"
                     /inference="COORDINATES: protein motif:HMM:NF021586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis BshC"
                     /protein_id="HEU5466479.1"
                     /translation="MPRVLATPITADVRRPRAGVPHRVSPDVLEAVLPGPGRDRLGKS
                     EVLVVTTGQQPGLFTGPLYTIHKALSAVALAARLERERQVPVVPVFWVAGDDADFAEA
                     NHCAFLDAAGDAHALVLRERPADAPQLSLFRERCGDDAARALEAFRIRTPDSEFKAAV
                     LGWLGSAYHPDANLADAFADAINGLLASHGLAVFRAHAPSAKRAAAPSLLKALDVVLP
                     DGLSPVLVEGKLGRDRLRADGKMFVTRRSEERFSRADLEGVARTAPERLSPNVLMRPA
                     VEAALFPTVAYCAGPGELQYLPDAAPCHAALGLTPQTPVPRWSGVLLESKVEKVLERH
                     RITVDDLKPPAGALEGRIAKGALPPETLELLERLRTSLTTDYDALAKSAATIDPTLER
                     TVQSTRNQALGGTQEIEKKLVASVKRTHETLTGQIVKARAALYPLGAPQERSLTIASF
                     LLRYGPSLVDELAREVARWTDAL"
     gene            complement(1510..2028)
                     /gene="amrA"
                     /locus_tag="VFU75_06285"
     CDS             complement(1510..2028)
                     /gene="amrA"
                     /locus_tag="VFU75_06285"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HEU5466480.1"
                     /translation="MLAAARMALEHALRRGLPVSINLPAVRRGAFVTLRIGGELRGCL
                     GHISADRPLGDLIPGLAVSAARDDPRFPPLTPDELGRVRIEISVLTTPAPVDPLDPES
                     ILIGRDGLIVERGSARGLLLPQVAPELRWVPAQFLDATCEKAGLPRGCWREPGTRVLT
                     FQADVFDEESKE"
     gene            complement(2054..2479)
                     /locus_tag="VFU75_06290"
     CDS             complement(2054..2479)
                     /locus_tag="VFU75_06290"
                     /inference="COORDINATES: protein motif:HMM:NF014629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEU5466481.1"
                     /translation="MKYRVVAVGRMKDAALRAACDGYLERLSHYAKVDESEVKDEARL
                     LGVIPAGTRLVALSRTGDPWSSRDLANQTERWEREARDVVFAIGGATALPDDVMKRAE
                     DVWSLSALTLPHELARVVVLEQLYRAYTMRRGEPYHRGP"
     gene            complement(2479..3765)
                     /locus_tag="VFU75_06295"
     CDS             complement(2479..3765)
                     /locus_tag="VFU75_06295"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HEU5466482.1"
                     /translation="MSDAIIRPEKDTFLAEENPFEAMMERFDHAAKLLNLDPGLYKIL
                     RHPEKQLIVSVPVLRDNGDVEVYTGYRVLYNTSRGPAKGGIRFDLNVTLDEVKALAAW
                     MTWKCAVVNIPFGGAKGGVICDPTTMSMAELERVTRRYTSGIIDVLGPDSDVPAPDVN
                     TNERVMAWIMDTYSMHKRHTVTAVVTGKPVEMGGSLGRREATGRGCMFVTREALKRIG
                     IPMNQARVAVMGFGNVGSISADLLAKEGATIVAVSDKSGGLINPKGLDVPDLIQWVRE
                     HRQLAGYPKAEAITNEQLITCDCDVLVPAALENVITSKNAPKIKAKIICEGANGPTTP
                     GADKILQDKGIFVIPDILANAGGVTVSYFEWVQDRGGYFWDEATVNNRLEQIMVKSFN
                     DVVALADKHKVNTRIAAYMLSIDRVAAMHRLRGMYA"
     gene            complement(3762..4799)
                     /locus_tag="VFU75_06300"
     CDS             complement(3762..4799)
                     /locus_tag="VFU75_06300"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HEU5466483.1"
                     /translation="MIRLVRWLWEGEGITGWIVRVAFLPLAVVYRAVMWGREIAYRHG
                     LVMTSSLPLRSISVGNLTVGGTGKTPVSAWLADWFLRHGARPAILLRGYGDDEPMVHA
                     RLVPKAVVQANPDRRAGAMAAARAGADLLVLDDAFQTLGIRRDLNLVLVAVEHLAYSP
                     FPLPAGPWREGWGGLGRADGIIVTRKRESAESAAVLADRLAKRWPKTPVGIAHLAISG
                     FEGLRSGQPLAGETLAGRRLLAAAGIADPDSFAAQLRERAGKSGAVQLVTYQDHHAYS
                     DRDVAELLSAATTADYVVVTEKDAVKLRSRWPAEAREPLVATLGVQWERGQEAFEALL
                     STLINSSTPAI"
     gene            complement(4796..5422)
                     /locus_tag="VFU75_06305"
     CDS             complement(4796..5422)
                     /locus_tag="VFU75_06305"
                     /inference="COORDINATES: protein motif:HMM:NF015961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HEU5466484.1"
                     /translation="MKLTFAEPVLSKVGQWLIRLLARTWRFRVSGEGPVTELIAQRRP
                     YILALWHSRILPLLFSHRDRGIVLLISKHRDGGHLADLASGLGYSAVRGSTNRGGEIG
                     LLGLIRALEVGNIVAVTPDGPRGPAEKVQAGIVSAARHASAAIVPITANASAEWRLPT
                     WDRMRIPKPFARVNVVFGAPIVLGPAKDDARRALGDLERVLTALGASS"
     gene            complement(5419..6534)
                     /gene="lpxB"
                     /locus_tag="VFU75_06310"
     CDS             complement(5419..6534)
                     /gene="lpxB"
                     /locus_tag="VFU75_06310"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HEU5466485.1"
                     /translation="MTASPRFYVSAGEPSGDAHAAPVVAALRRRLPGLEVEAFGGPAL
                     EGAGAKVLDRMEAMSVVGFVEALGTIPAHRRLAARTRESLAAGRYRAVMLVDYPGYHL
                     RFAPQVTALKVPVLYYIAPQLWAWAPGRIKRMRGNVRQLAVILPFEERFFRERGVPAT
                     FVGHPLKDRPPLPSRDEARRILKLDARRPVLGIFPGSRRQEVAHMWTRFRDGALEVRA
                     AMPDVQLVVAGTTRAEYPDPREIPIAYGDPGLVFAAADAGLCKSGTTTLEAALADLPM
                     AIAYRMNGLSYFIARRLLRVPNVGLVNLVAERELAPELLQGQANPRALARSVMPLLDR
                     DGHPARAQREGLALVRERLGPPGAAERVADLAAELLG"
     gene            complement(6531..7508)
                     /locus_tag="VFU75_06315"
     CDS             complement(6531..7508)
                     /locus_tag="VFU75_06315"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5466486.1"
                     /translation="MTAPRVRIGVIGTGALGYHHARLLRHIEGAELAGIYDRNPERLA
                     HVAKELGAVPHASLDALLDKVDAVSVVVSTPAHAEVGLAALSRGISCLIEKPLADSVS
                     GAEQLVAAAKKHGAKLQVGHVERFNRAVRAAMPYLEDPRFFQCDRLAPFTPRGSDVAV
                     VLDLMIHDLDLILELVGKDVTDVRATGAPLLTPHVDIANARVEFSNGAVAIVTASRIS
                     RERTRKLRIFQPTGYFSLDLAQGTGHFFRLKTGWKGLGATRIEDIVEHAKLDAPEAEP
                     LKLELENFVRAVRGEGEVAVSGEAGAQALVLATRVVDAIQASPLATLRT"
     gene            complement(7505..8272)
                     /gene="lpxA"
                     /locus_tag="VFU75_06320"
     CDS             complement(7505..8272)
                     /gene="lpxA"
                     /locus_tag="VFU75_06320"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HEU5466487.1"
                     /translation="MIHPTALVHAKAELGREVEVGPYAVIGPQVTVGDGSKIAAHAVV
                     ECNARIGAGVQIGVGTVVGGPPQDLKYKNEETWVEIGDQTVLREYSTVNRGSTATGKT
                     TIGARCFLMTYVHIAHDCHLGNDIIIANGVQMAGHVTIGDRANISGLTPIHQFCRIGT
                     FAFVGGGSRVNQDVPPYVKAVGNPLQLYGLNSVGLQRAGFSAEVKLALKRAYRLLFNS
                     DFNITQGIARARAELPALPEVETFLTFIETSQRGVLV"
     gene            complement(8269..9588)
                     /locus_tag="VFU75_06325"
     CDS             complement(8269..9588)
                     /locus_tag="VFU75_06325"
                     /inference="COORDINATES: protein motif:HMM:NF009667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HEU5466488.1"
                     /translation="MSSRRSRARKGIPIARRTLKQPVTLSGVGLHTGAHTEAHLLPAT
                     SGQGIAFRRTDLADKPLVPARLTEVEALERRTAIGHGDATIHTVEHLLSALGALEVDD
                     CIIELSGPEPPALDGSVGPYFKALMEAGTADHDGEPVLLTVQAPFTVTEGDASYVVAP
                     AKSYRLTATVEFAHPMIGRQAGTYEITPEAFAKELANARTFGFASEVDALRAKGLIKG
                     VNKDNAIVLDEKTVVQGGPLRWPDEFVRHKAADIVGDLALTGARIRAHIVATRPSHVG
                     NVALARALSRTAKRTGDARYDIGRILDILPHRYPFLLVDRIIEVEGDRRIVGIKNVTI
                     NEPFFQGHFPGHPIMPGVLIIEAMAQVGGMLLMGHFEGQNVEDKVVYFMSLDNVKFRR
                     PVLPGDQIRFELEMVQFRGRTCKMKGVGYVDGQVVAEAEMMAIVTDK"
     gene            complement(9542..10594)
                     /gene="lpxD"
                     /locus_tag="VFU75_06330"
     CDS             complement(9542..10594)
                     /gene="lpxD"
                     /locus_tag="VFU75_06330"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:TIGR01853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HEU5466489.1"
                     /translation="MPAEGHRPLSAQDVAALTGGRLVGPGHVTVARVAPLERAGAGDL
                     AFLASRSYLPYFERAQAAVVLCRPEFADLPQGPPTRIVVADPHAALLDVLPILYPEPA
                     WVPGVDATAVIGRGSTWTDPVAIGPYVVLGENVRLGQNVRIDAGCVLGDGVIVGNDVH
                     LYPQVTCYTGTVIGDRVMIHSGTRLGADGFGYLPGKGGALPRKIPQVGRCVIGDDVEI
                     GANTTVDRGSVDDTIVGRGTKIDNLVMVAHNVRIGERCLIAAMVGIAGSTRVGDDVFL
                     AGQVGLADHINVGSRVRITVQSGVIGDIEDGATVTGYPARNHRDYLRAQAALYRLAPL
                     VNELEALARPEGDSDR"
     gene            complement(10603..11166)
                     /locus_tag="VFU75_06335"
     CDS             complement(10603..11166)
                     /locus_tag="VFU75_06335"
                     /inference="COORDINATES: protein motif:HMM:NF015871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HEU5466490.1"
                     /translation="MKRVLVVCLALAATRTLSAQAAHAQTPAPKTVTGLKIAYVIPQQ
                     ILDSTPGYVTAESTLAREVQGYRDEVQKMQQQMDSAVQALDQQSIALSPAAKQAKQKD
                     LQNMQQRFDARTQELNQKAQQRQQELMAPIQQKIRTIIEAVRLEGGYSMVFNGDPSAS
                     GLLAADPALDITRTVVARIKQLPAQSN"
     gene            complement(11202..13607)
                     /gene="bamA"
                     /locus_tag="VFU75_06340"
     CDS             complement(11202..13607)
                     /gene="bamA"
                     /locus_tag="VFU75_06340"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HEU5466491.1"
                     /translation="MIRRVSLGAVCGLLCLLPQIARGQQAAQIIPDSIAVLGIKRVTR
                     QTVLSTSGFAVGRVTGYRDVQHALEALYASGQFQDVRVAADTSAGRSILLVTVKERPI
                     LIKWTVKGTEKISERTVRERVTLFEGRPLDPAAVARSAARIDSLYRGDGYYLASVDPK
                     WIYEADSSRVRVVFEIDEGRRVAIARVNFEGNHAFTAQTLVDQMSSKPEGFFFWRNGE
                     YDEDKLQKDLTQKLPDFYGARGYVDFQVLGDTLIVDDTTGKATLVVRLSEGQPYKIGT
                     FEIAGNRHFTTEEIQGLYPFSSSRRTGLLGLGGTEHGAAYFNQAKWDDATNKVYTAYR
                     DDGYLYVQIQNDVVRRVERDGTPVVDLRWFVQEHQPAIVNKIDIVGNDVTHEAVIRDA
                     IEMIPGDVFRQNALLASYQNISNLGFFQQPLPIPDTRPANDQGDVDVIFRVEEKRTGN
                     VNFGASVGQGTGLGGFIGLDEPNLFGRGKRGHFQWQFGQNITDFDISYTDPAIMLSRI
                     SGTLDLHDSRYRYTIADLGQLRRRGGSLQFGFPLFDARKHVRLFLSYSLDDQSFIGSA
                     LNPAFGTSFGCSNCLRSTLGTSILRDTRVELPFPTGGTMTSIGVSQSGGVLGGTGNFQ
                     RVDIESHWYAPLGTLGGKGAGANPPKFVLGLSTRSGFVFGKSPFFDQLFTMGGTQFGI
                     PLRGYDEFSVTPKGYDPSAAGGQASANAFGNAFFLMNAEIGLRLSQAFYVSTFFDAGN
                     VWARPAQYDPTRLFRGAGVGVAVVSPLGPIGLDLGYGFDKVNLAGQPAPGWKLHFKLG
                     NFF"
     gene            complement(13604..16084)
                     /locus_tag="VFU75_06345"
     CDS             complement(13604..16084)
                     /locus_tag="VFU75_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEU5466492.1"
                     /translation="MNGYNFTDRVRKVLQMAREEAARLHHEYVGTEHILLGLIREGEG
                     VAAAVLTNLNVDLDEIQQKIEETVKKGKAAAAAGPDLPYTSRAKKVLELAMAEARDLN
                     HSYVGTEHLLLGLLREEKGIAAQVLTDAGVNLEQAKAETLRLLGSEMPAAPAGGPPAG
                     QPASPKSEKKSKTPALDHFCRDLTQLANEGALDPTIGRQKEIERVMEILSRRKKNNPV
                     LIGEPGVGKTAIVEGLAQLIAGGQCPDALKDHRVLSLDMAAVIAGTKYRGQFEERLKA
                     VINEIAQNKNIILFIDELHTLVGAGAAEGAVDASNMLKPALARGELQCVGASTLNEYR
                     KYIEKDGALERRFQTVIVDPPTVDETIEILKGLRKRYEDHHRVIIPDDTLASAARLSE
                     RYITDRFLPDKAIDVIDEAGARARLATQVPPPEAAALKERLEKINTEKEGAVRDQNFE
                     RAASLRDQERELQMEIRRKQEEWEKERQTRRPTIDEEGIAFIVSRWTGIPVTRLQEAE
                     TARLLRMEDEVHASVVGQNEAIEVIARAIRRSRAGLKDPKRPIGSFIFSGPTGVGKTE
                     LARALAKFLFADASALIRVDMSEYMEKFSVSRLIGAPPGYVGYEDSGTLTKAVRRKPY
                     AVVLLDEIEKAHPDVFNILLQVLDEGHLTDNYGRVIDFKNTVVIMTSNVGARDMVKGK
                     TMGFAQPDGEASFARMAEKVKEEINKVFNPEFLNRLDDVIVFHPLNRQHIAQIVVILL
                     KDVQKRLGDEELTLRLTEAASNFLVDHGYDEHYGARPLKRAIQRFVEDPLSEKILVGE
                     FARGDEIEVDVAPDKERLAFRALSGSKA"
     gene            complement(16098..17171)
                     /locus_tag="VFU75_06350"
     CDS             complement(16098..17171)
                     /locus_tag="VFU75_06350"
                     /inference="COORDINATES: protein motif:HMM:NF012443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HEU5466493.1"
                     /translation="MMDLSLLPDGGLGWLDASGPKSQIVLSTRIRLARNLAGHTFSQR
                     STDDERLAVLDRVLEAAAASERLKRNVSFRLDALERGDRQLLHERHLVSKELAGLDQD
                     TRPRPGAALVAQSPVGVMVNEEDHLRLQGMWSGFALDEAYAQLESIDADLGRLLPFAF
                     HPEFGYLTSCPTNAGTGLRASVLIHLPGLVLTKEIGKVLQGLAQVGLTFRGLYGEGSE
                     VVGNFFQLSNQTTLGKSEGELLDHLGKIVRQVIEYEDQARQMLLRTAPDEVADKTWRA
                     YGLLRHARRLTFEEAMNLLSGVRLGVGLDLIPGLSVYTLNKLLIYTQPAHLAALEGRT
                     GGDPELPTVRATYVRRLLESESP"
     gene            complement(17168..17662)
                     /locus_tag="VFU75_06355"
     CDS             complement(17168..17662)
                     /locus_tag="VFU75_06355"
                     /inference="COORDINATES: protein motif:HMM:NF014232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HEU5466494.1"
                     /translation="MLCDHCKERDAVINLTQVEHDSKVTLHLCEQCAAEKGVETGAAA
                     LKSPLGGFIMAMGKTAGSLLPATVDGTPCPNCGGTLKDFRETGKLGCAQCYAAFEVHL
                     RDLLRRLHGSSQHVGERYQQPGGEDADPAHRLLELRHQLRQAVDAENFELAAELRDQI
                     RVLE"
     gene            complement(17662..18363)
                     /locus_tag="VFU75_06360"
     CDS             complement(17662..18363)
                     /locus_tag="VFU75_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5466495.1"
                     /translation="MNGLLESRGLRKVFAGGDGQPLEVLRGVDIDVRRGELIAIVGTS
                     GAGKSTLLHLMGALDRPTAGDIWLEGSRFADLDANALAELRNRRIGFVFQFHHLLREF
                     SALENVMMPMLIGGASERQARSRAEERLSAVGLAGRMTHRPAELSGGEQQRCAVARAL
                     VHDPALVLADEPSGNLDHANAEVLHETFFRLARDFETAVVVVTHNRQLAARADRILWL
                     EDGRLGAVPSVEAIP"
     gene            complement(18360..19616)
                     /locus_tag="VFU75_06365"
     CDS             complement(18360..19616)
                     /locus_tag="VFU75_06365"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5466496.1"
                     /translation="MIGALELRIALRYLTSRRSSGLLSFITVIAVGGVFVGVAALMLV
                     LGVMNGLQSDLRDKILVGSPHLRVLTYGEGLRLDDWRRVLDSVRATKGVTAAAPFVMT
                     QAGVTTGHDYAEGVVVYGIEPDTGTRAVTTFAQHFIKGDLSFRTTRPDVEGGVALGTR
                     LAGKISAYVGSVVTLVSFTGRSSFSASAGTFVPQYHRYEVTGIFDTGMYIYDDSYVVL
                     SRSAAQRLAGLDTAVTGIEVQLADPGHARTFGAALETHLGYPYRTMDWQTQNGALFSA
                     LQLEKLTMGLVVFLICVVAAFNVVGMLTMVVRDKTREIGILLTMGLRRASIRRVFLAQ
                     GIIIGLTGTALGAVTGIIVGVMVNQGRWIPIDPSIYFVDHLPVHFQALDILAVIAASL
                     LVATIAPLFPAFRAGDLEPVTAIRYE"
     gene            complement(19613..21082)
                     /gene="lysS"
                     /locus_tag="VFU75_06370"
     CDS             complement(19613..21082)
                     /gene="lysS"
                     /locus_tag="VFU75_06370"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000369668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEU5466497.1"
                     /translation="MTTSVEAARREKLEELARKGVPAFAYRFDRTGLAQPALQGFTDG
                     DETRHRLAGRLVLMRGMGKTTFAHLEDASGKIQLYFKSDLLGERYEIIKLLDLGDVVG
                     VAGPLFKTKTGEVTLRVEELTLLAKSLRPLPLGKEDAQGVRHGELTDPELRARQRYAD
                     LAAHPEARAVFHARARVVSYVRRFLDGRGYLEVETPVLQPLYGGAAATPFTTFYEALG
                     ATFYLRIADELYLKRCIVGGLEKVYEIGHDFRNEGVDRWHNPEFTMAEWYEAYADYED
                     MLNLTEAMVAGAAVEVTGEETLERGGATLSFKRPFARRDFYDLVKETAGVDLGAADEA
                     TLRGALKRKGVTDADQYSGAKLMDEVFKTFAEPTLVQPTFVMNHPVALSPLAKRKRDR
                     EDRVERWELFIQGREIANAFSELNDPDEQRRRFEEQVRFRERGDSEAHQIDEDYLRAL
                     EYGMPPTGGVGLGIDRLVMLLTDRTNIRDVILFPMLRPE"
     gene            complement(21079..22180)
                     /gene="prfB"
                     /locus_tag="VFU75_06375"
     CDS             complement(join(21079..22122,22124..22180))
                     /gene="prfB"
                     /locus_tag="VFU75_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_319006967.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEU5466498.1"
                     /translation="MANRFSEFQERLAELRSFLDVDGKTKRLAELEHAMAEGAIWSDQ
                     ERAKKVVEEVKTLKRQLEPFHALKKRVDDGFELSQLLEAESNVDLEMQRSLEAEADAA
                     ARALNAIELQHMLRGPDDPRDALLTIHPGAGGTESQDWAEMLMRMYTRWAERRGFDIE
                     VLDLLEGEEAGIKSAEIEIRGQYAFGLLRAEKGVHRLVRISPFDAQARRHTSFASVFV
                     YPVVDDDIEIEIKEDDLRIDVFRASGKGGQHVNKTSSAVRITHLPSGIVVACQNERSQ
                     FKNKATALKMLKSRLYEKALEEQEAKKAEVDKQKGDIAFGSQIRSYVFQPYTMVNDHR
                     TELKVGDVQKVMDGDLDQFIEAFLKKQAGKAA"
     gene            complement(22290..22634)
                     /locus_tag="VFU75_06380"
     CDS             complement(22290..22634)
                     /locus_tag="VFU75_06380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466499.1"
                     /translation="MPAATAPPVATAAPAGGAELRKTGPLRPVNPFMVQDPKQKARRL
                     ARALISDMLVYHPDKRMQGTRDGTLPQLFKDEIEKSWLEYVEQVGQEMASSTPFWTDA
                     LNELLAGGQKVF"
     gene            22633..23175
                     /locus_tag="VFU75_06385"
     CDS             22633..23175
                     /locus_tag="VFU75_06385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466500.1"
                     /translation="MAGAGGAGGDAGIAGAAGGGAAGRAGAAGIAGAAGMRGAVAGGA
                     TIGDLAGGAIIGGATGGRTGAAGGPTDGRGGIANAGGCATGGAGTTGGGGGAGVNTGA
                     AGGGSGGAGAAIGAGAGVAGGAGGGEGGGGGAAAVTAAGAGAGTATGWVPLAETGKTL
                     EQTEHRARMPPAGTLAGSTR"
     gene            complement(23229..24191)
                     /locus_tag="VFU75_06390"
     CDS             complement(23229..24191)
                     /locus_tag="VFU75_06390"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU5466501.1"
                     /translation="MRTRQTILFFSPTGRPVPDTLLPWITGKQTGLVQVSDPAVIQQM
                     VLRALPLLVLVDADSDGAASLELCSRLKGDPYTAIVPLAAVAGRHVTEKVREWFANGA
                     DEVLTPLLEPAEQEVRLDGLLNRTARDVAVNPSTRLPGTTEIEREIRRRMEAGEWFAV
                     CYADLDHFKEYNDRYSYYDGDRVIYLLSRILHDVVKGMTGPRGFIGHIGGDDFIFMIP
                     VDAITEVCGEILTVFDTLIPYQYNEQDRRAGYYFGKDRRGQLHRVPLMTLSIGIVTNQ
                     HRRFAHPAQVSELATEMKSYAKTQAGSVFVVDRRRDHESGETPE"
     gene            complement(24264..25043)
                     /locus_tag="VFU75_06395"
     CDS             complement(24264..25043)
                     /locus_tag="VFU75_06395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466502.1"
                     /translation="MHRRWLALTLFIGSALAPPLRAQVDSVALREKSWVRPLASLVLP
                     GTGQALSGQDRAAVYGAVEIYVLSRYIQLQHDANLNAERYHQLAFAVAQRGFASVRRD
                     TIFEYYETMEKFTASGRFNTGAGPGLNPETDPTTYNGSVWLLARRTYWADPNVPPPPN
                     SPQYQAALSFYVQHAVGPAYQWTWQSASLQQQTFRDLIRTSDNGYRNAQGALGLLLAN
                     HVVSALDALISNRLTTATHRTTSLHTVIGPDFSLLAVHVAF"
     gene            complement(25043..25981)
                     /locus_tag="VFU75_06400"
     CDS             complement(25043..25981)
                     /locus_tag="VFU75_06400"
                     /inference="COORDINATES: protein motif:HMM:NF033709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorV/PorQ family protein"
                     /protein_id="HEU5466503.1"
                     /translation="MLLPIGAQSVGMGQAGVTLQGRGESVFWNPAGLASLPAAEFAVS
                     SANFVAGPGTAITMFVPRRGIGTFGVGVFLLDYGEQDVGSDSLSAIAKIFPRNVEYLA
                     SFATSLAGAVSVGVTYKLVQFTIDCQGNCAGLPNGSDALTHAFDVGTQVAVGSGGALR
                     FGAAVKDIGFKLQVNNAAQSDPLPARLSLGALYRIDLHPADQGGAPTLAAPDSGRLDL
                     SSALNQVDIRLAADLDRPWDNSAPPEMRFGIDVGYHETIRVRSGYAFAQQGLSGPSIG
                     MGVHTGSIAIDFARTFVQNTDLLGANPTFISFSLTF"
     gene            complement(26088..26258)
                     /locus_tag="VFU75_06405"
     CDS             complement(26088..26258)
                     /locus_tag="VFU75_06405"
                     /inference="COORDINATES: protein motif:HMM:NF015899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HEU5466504.1"
                     /translation="MSLSPDLLAILVCPKCKGPLEHRTAPAETLTCPACRLSYPVEDD
                     IPVMLVDEAKPI"
     gene            complement(26255..27103)
                     /gene="era"
                     /locus_tag="VFU75_06410"
     CDS             complement(26255..27103)
                     /gene="era"
                     /locus_tag="VFU75_06410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEU5466505.1"
                     /translation="MTRCGVVALVGRPNVGKSTLLNAFVGQHLAIVSPKPQSTRLPVV
                     GLVTTAETQFIFTDTPGLLDPEYRLQEVMRAAALATLEDADVIAYLHPLGDGDAPPLV
                     EVAGLPKQPRAPVVTVYTKCDLRPTVATPLGGLAISATSGAGVAALRDALGAHLPESP
                     FLYPDDDLATQPLRFFAAEYVREAAFGELREELPYSLACDVDEFRESETPVYIRATVY
                     VERDSQKGMVIGAGGKVIKAIGQKARARIEALTGQPVFLDLHVKVMPRWRADASSLKR
                     LGYSIG"
     gene            complement(27162..27278)
                     /gene="rrf"
                     /locus_tag="VFU75_06415"
     rRNA            complement(27162..27278)
                     /gene="rrf"
                     /locus_tag="VFU75_06415"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(27347..>27822)
                     /locus_tag="VFU75_06420"
     rRNA            complement(27347..>27822)
                     /locus_tag="VFU75_06420"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         4544 a         9193 c         9422 g         4663 t
ORIGIN      
        1 tggtgaatcc accaggatgg ccgtgatcgg gatgaggagg gcgatgaaca gcaccgcgcc
       61 gaggacgatt tcccacatac gaaggattct agagagcgtc ggtccagcgc gccacctcgc
      121 gcgccagctc gtccacgagg ctcgggccgt agcgcagcag gaaggaggcg attgtcagcg
      181 aacgctcctg cggcgcgccg agggggtaca gcgcggcgcg tgccttcacg atctgcccgg
      241 tgagggtctc gtgcgtccgc ttgacgctgg cgaccagctt cttttcgatc tcctgggtgc
      301 cgccgagggc ctggttgcgg gtcgactgca cggttcgttc gagcgtcgga tcgatcgtgg
      361 ccgcggactt ggccagggcg tcgtagtcgg tggtcaagga ggtccgcaga cgctcgagca
      421 gctcgagtgt ctccgggggc agcgccccct tggcgatgcg gccctcgagc gctccggcgg
      481 gtggcttcag gtcatccacg gtgattcgat gccgttcgag caccttctca accttcgact
      541 caagcaggac cccggaccag cgtgggacgg gcgtctgcgg ggtcaggcca agggcggcgt
      601 gacaaggcgc tgcatcggga aggtactgga gctccccggg gccggcgcag tacgcgaccg
      661 tgggaaagag ggccgcttcg acggccggac gcatcaggac gttcggtgaa agccgctcgg
      721 gcgcggtcct ggcgactcct tcgagatcgg cgcggctgaa acgctcctcg ctgcgtctgg
      781 tgacgaacat cttcccgtct gcccgcaggc ggtcccgccc cagcttgcct tccaccaaca
      841 ccggcgacag cccgtcaggc agtacgacgt cgagcgcttt caggagcgac ggggcggcgg
      901 cccgtttggc ggaaggggcg tgtgcccgaa acaccgcgag accgtgtgat gcgaggagtc
      961 cgttgatcgc gtccgcgaag gcgtccgcca gattcgcgtc ggggtgatag gcgctgccga
     1021 gccaaccgag gaccgcggcc ttgaactcgc tgtcgggcgt cctgatgcgg aaggcttcga
     1081 gagccctggc ggcgtcgtct ccgcagcgtt cccggaaaag ggagagctgg ggagcgtcgg
     1141 caggacgttc acgcaggacg agagcatgcg catctcccgc cgcatcgaga aaagcgcagt
     1201 ggttcgcttc cgcgaagtcg gcgtcgtcgc ccgctaccca gaacacgggc accaccggca
     1261 cctgacgctc ccgctccaat cgggcggcca gggccaccgc ggagagcgcc ttgtgaatgg
     1321 tatacaaggg cccggtgaac agcccgggct gttgcccggt ggtcactacc aggacctcgc
     1381 tcttcccaag ccggtcccgc cccggaccgg ggagcacggc ctcgagcacg tcaggtgaca
     1441 cgcgatgcgg cacgccggcc ctgggcctgc gcacgtcggc ggtaatcgga gtcgcgagca
     1501 cccgcggcat cattccttgc tctcctcgtc gaagacgtcc gcttgaaacg tcagaacccg
     1561 ggtccctggt tcacgccagc aaccccttgg gagccccgcc ttctcgcagg tcgcgtccag
     1621 gaactgggcc ggtacccacc ggagctccgg ggctacctgg ggcagcaaga gacctcgggc
     1681 gctcccccgt tcgacgatca acccgtcccg cccgatcagg atcgactcgg ggtccaaggg
     1741 gtcgaccggc gcgggggtcg taagcaccga aatctcgatc ctcacgcggc ccagctcgtc
     1801 tggggtgagc ggcggaaacc ggggatcgtc tcgcgccgcc gaaaccgcca atccaggaat
     1861 caagtcgccg aggggccgat cggcgctgat gtgcccgagg catcctcgaa gctcaccccc
     1921 aatccggagg gtcacgaaag ctccccggcg aacggcggga agattgatcg agaccggcaa
     1981 cccgcggcgt agtgcgtgct ccagggccat gcgggctgcc gcgagcaacc cgtgccgtgc
     2041 ttcgggcgtg agatcagggt ccgcggtgat aaggctcccc ccgcctcatc gtgtaggcgc
     2101 gatagagctg ctccaacacc accactcggg ccagctcatg gggcaacgtc agagcggaga
     2161 gggaccagac gtcctcggcc ctcttcatca cgtcatccgg caacgccgtg gcgccaccga
     2221 tggcgaagac cacgtcccgg gcctcccgct cccagcgctc ggtctggttc gccagatcac
     2281 gcgacgacca cgggtccccg gtccgcgaca acgcgacgag gcgcgtcccc gccgggatga
     2341 cccccagcag acgcgcctcg tccttcactt ccgattcgtc gaccttggcg tagtgggaga
     2401 gccgctccag gtacccgtca cacgccgcgc ggagtgccgc atccttcatg cggcccaccg
     2461 cgacgacgcg atacttcatc aggcatacat cccacgcaac cggtgcatgg cggccacccg
     2521 gtcgatggac agcatgtatg ccgcgatccg ggtgttgacc ttgtgcttgt ccgccagagc
     2581 caccacgtca ttgaacgact tcaccatgat ctgctcgagg cggttgttca cggtcgcctc
     2641 atcccagaag tagccgcccc ggtcctgcac ccactcgaag tagctgacgg tcacgccgcc
     2701 ggcattcgcg aggatgtccg gaatgacgaa gatccccttg tcctggagga tcttgtcggc
     2761 gcccggtgtg gtgggcccgt tcgcgccctc gcagatgatc ttcgccttga tcttgggcgc
     2821 gttcttggac gtgatgacgt tctcgagggc ggcgggcacc agcacgtcgc agtcgcaggt
     2881 gatcagctgt tcgttggtga tcgcctccgc cttcggatac ccggccagct ggcggtgttc
     2941 gcgtacccat tgaatcaggt cggggacatc gaggcccttg gggttgatca gcccgcccga
     3001 tttgtccgac acggccacga tggtcgcgcc ttccttggcg agcaggtcgg cggagatcga
     3061 gccgacgttc ccgaagccca tgacggcgac gcgcgcctga ttcatcggga tgccgatgcg
     3121 cttcagcgcc tcgcgggtga cgaacatgca cccgcgcccg gtcgcctcgc ggcggcccag
     3181 cgacccaccc atctcgaccg gcttcccggt gaccaccgcc gtcactgtgt gccgcttgtg
     3241 catcgagtag gtgtccatga tccacgccat cacccgctcg ttggtattca cgtcgggagc
     3301 cggcacgtcg gagtcgggtc ccaatacgtc gatgatcccc gaggtatagc ggcgggtcac
     3361 ccgctccagc tcggccatgg acatggtcgt gggatcgcag atgacgccac ccttggcccc
     3421 accgaacggg atgttcacca cggcgcactt ccacgtcatc cacgccgcga gggccttcac
     3481 ctcgtcgagc gtgacgttca agtcgaaccg gatgccgccc ttcgccggac cgcgcgacgt
     3541 gttgtagagg acgcgatagc cggtgtagac ctcgacgtcg ccgttgtccc ggagcacggg
     3601 caccgagacg atcagttgct tctcggggtg ccggaggatc ttgtagaggc cgggatccag
     3661 gttcaggagt ttggccgcgt gatcgaagcg ctccatcatg gcttcgaacg gattctcctc
     3721 ggccaggaac gtgtctttct cgggacggat aatcgcgtcg ctcatatcgc cggagtggag
     3781 gaattgataa gggtggacaa cagcgcctcg aaggcctctt gtccccgctc ccattgaacg
     3841 ccgagtgtcg ccaccagcgg ctcgcgcgcc tctgcgggcc aacgggaacg gagtttcacg
     3901 gcgtcctttt cagtgactac aacataatcg gctgtggtag cggcgcttag cagttccgcc
     3961 acgtcacggt cgctgtaggc gtgatggtct tggtacgtca caagttgcac tgccccggac
     4021 ttccccgcgc gctcccggag ctgtgctgca aaggagtcgg ggtcggcgat accggccgcg
     4081 gccagcaggc gccgtccggc gagagtctcc cccgccaaag gttgacccga ccgcaagccc
     4141 tcgaatccgg agatggcgag gtgcgcgatg ccgacgggag tcttgggcca cctcttggcg
     4201 aggcggtcgg cgaggacggc cgcactctct gcggactccc gttttcgggt gacgatgatg
     4261 ccgtcggcac ggcccaatcc gccccagccc tcccgccagg gtccggcggg aagtggaaac
     4321 ggggaatagg ccaagtgttc gaccgcaacc aacacgaggt tgaggtcgcg ccggatcccc
     4381 agggtctgaa aggcgtcatc gagcacgagg aggtcggcgc cggcgcgcgc ggccgccatg
     4441 gcgccggccc gccgatcggg attggcttgg accacggcct tcggcacgag ccgggcatgc
     4501 accatcggct catcgtcccc gtagccgcgg agcaggatcg ccggtctggc accatgtcgc
     4561 aggaaccagt ccgcaagcca cgcggatacc ggtgtcttcc ccgttccgcc gacggtgagg
     4621 ttcccgacgg agattgaccg gagtgggagg gacgacgtca tcaccaaccc gtgccggtag
     4681 gcgatctcgc ggccccacat caccgctcta tagacgacag cgagcggcag gaaagccact
     4741 ctcacgatcc acccggtgat gccttcaccc tcccagagcc accggacgag gcggatcacg
     4801 aagatgcccc aagagccgta agcacccgct cgagatctcc caatgcacgg cgcgcatcgt
     4861 ccttggcggg cccgagcacg atcggtgcgc cgaagacgac gttcacgcgt gcgaagggct
     4921 tcgggatccg catccgatcc cacgtcggca gtcgccactc ggcgctcgcg ttggcggtga
     4981 tcggcacgat ggccgccgac gcatgccgcg ccgccgatac gatccccgcc tgcaccttct
     5041 cggcgggtcc gcgggggccg tcaggggtca ccgccacgat gttccccact tcgagtgcgc
     5101 ggatgaggcc gagcaggccg atctccccgc cccggttggt cgacccccgc acggccgagt
     5161 aaccgagccc cgaggcaagg tcggcgaggt ggccgccatc ccggtgcttg ctgatcagca
     5221 ggacgatgcc gcgatcgcga tgcgagaaga gcaacggcag gatgcggctg tgccagagcg
     5281 cgaggatgta agggcgccgc tgggcgatca gttccgtcac aggcccttcg cccgagaccc
     5341 ggaaacgcca ggtacgcgcg aggagcctga tgagccattg tcccaccttc gagaggacgg
     5401 gctcggcgaa cgtgagcttc accccaggag ctccgccgcg aggtcggcca cacgctcggc
     5461 ggcgcccggg gggccgagcc gctcacgcac cagggccagg ccttcgcgct gtgcacgggc
     5521 ggggtgtccg tcgcgatcga ggagcggcat gacggatcgt gcgagggcac gcggattcgc
     5581 ctggccttgg aggagctcgg gcgccagttc ccgctcggcc accagattga cgagtcccac
     5641 gttcggcact cggagcaggc gccgggcgat gaagtacgac agtccgttca tccggtaggc
     5701 gatggccatc gggagatcgg cgagtgccgc ctcgagcgtc gtcgttcccg acttgcaaag
     5761 tccggcgtcg gcggcggcga aaaccaggcc ggggtcaccg tacgcgatcg ggatttcgcg
     5821 ggggtccggg tactcggcgc gagtggtgcc cgcgactacc aactgaacgt cgggcatcgc
     5881 cgccctgacc tcaagcgccc cgtcccggaa gcgggtccac atgtgcgcga cttcctgccg
     5941 tctgctcccc ggaaagatgc cgaggaccgg ccgccgggca tcgagcttga ggatgcggcg
     6001 ggcttcgtcg cgggacggaa gcgggggccg gtccttgagc gggtgaccga cgaaggtcgc
     6061 cggaacgccg cgctcccgga agaagcgctc ctcgaacggg aggatgaccg cgagttggcg
     6121 cacgtttccc cgcatccgct tgatgcggcc gggagcccag gcccagagct gcggcgcgat
     6181 atagtagagg acgggcacct tgagggcggt gacctggggg gcgaagcgca aatggtatcc
     6241 ggggtaatcc accagcatga ccgcccggta gcgccccgcg gccaggctct cccgggtccg
     6301 ggcggccagg cggcggtggg ccgggatggt gccgagggcc tccacgaacc ccaccaccga
     6361 catcgcttcc atccggtcca gcaccttggc gcccgccccc tcgagggcgg gtccaccgaa
     6421 cgcttccacc tcgaggcccg ggagacgccg gcggagggca gctacgacgg gggcggcgtg
     6481 cgcatccccc gacggttcgc ccgccgagac ataaaagcgg ggagacgcgg tcacgtccga
     6541 agggtggcga gcggggacgc ctgaatggcg tcgacgacgc gggtagcgag gaccagggcc
     6601 tgggcgccgg cctcgccgga caccgcgacc tccccttccc ctcgaaccgc gcgcacgaag
     6661 ttctcgagct cgagcttcag gggctcagct tcgggcgcat ccagcttcgc atgctcgacg
     6721 atgtcctcga tgcgggtcgc ccccaggccc ttccaccccg tcttgaggcg gaagaaatgc
     6781 ccggtgcctt gcgccagatc gagcgaaaag tatccggtcg gctggaagat ccggagcttc
     6841 cgggtgcgtt cccgcgagat gcgggatgcg gtgacgatcg cgacggctcc gttcgagaac
     6901 tcgacgcggg cgttggcgat gtcgacgtgc ggcgtcagca agggcgcacc cgtggcccgg
     6961 acgtccgtca cgtctttgcc gaccagctcg agaatcaggt ccaggtcgtg gatcatcagg
     7021 tcgagcacca cggccacgtc ggatccgcgg ggcgtgaagg gcgcgagccg gtcgcattgg
     7081 aagaacctgg ggtcctcgag gtacggcatg gcggcccgca cggcccggtt gaagcgctcg
     7141 acgtgtccca cctggagctt cgccccgtgc ttcttcgccg ccgccaccag ttgctcggcg
     7201 cccgagaccg aatccgcgag cggcttctcg atcagacagg aaatgccgcg ggacaacgcg
     7261 gcaaggccga cctccgcgtg tgccggagtc gacaccacca cgctcaccgc gtccaccttg
     7321 tcgaggaggg cgtcgagact ggcgtgcggc accgccccga gctccttggc cacgtgggcc
     7381 aggcgctcgg gattccggtc gtagatcccg gcgagctcgg cgccctcgat gtggcggagg
     7441 aggcgggcgt ggtggtaacc aagggcgccg gtgccgatca cgccgatccg cacccgcggc
     7501 gcggtcacac caacacccct cgctgcgagg tctcgatgaa ggtgaggaag gtctcgactt
     7561 cgggaagcgc ggggagctcc gcgcgagcgc gcgcgatgcc ctgcgtgatg ttgaagtccg
     7621 aattgaacag gagccggtac gcgcgcttga gggcgagctt cacctcggcc gagaacccgg
     7681 cgcgttgcag gccgacggag ttgaggccgt agagctggag cgggttcccc acggctttca
     7741 cgtacggcgg cacatcctgg ttcacgcgcg atcccccgcc gacgaaggcg aaggtgccga
     7801 tccggcaaaa ctgatggatg ggggtgagcc ccgagatgtt ggcgcggtcg ccgatggtga
     7861 catgtccggc catctgcacg ccgttcgcga tgatgatgtc gttgccgagg tggcagtcgt
     7921 gggcgatgtg cacgtaggtc atgaggaagc agcgcgcccc gatcgtggtc tttccggtgg
     7981 cggtgctgcc gcgattcacc gtggagtact cacgcaggac ggtctgatcc ccgatctcga
     8041 cccaggtctc ttcgttcttg tacttgaggt cctgcggcgg tccgccgacc acggtgccga
     8101 ccccgatctg tacgcccgcg ccgattctcg cattgcactc gaccacggcg tgcgcggcga
     8161 tcttcgaccc gtccccgacg gtcacctggg gaccgatgac ggcgtacgga cccacctcga
     8221 cctcgcggcc gagctcggcc ttcgcgtgga ccagcgcggt gggatgaatc atttgtcggt
     8281 tacgatggcc atcatttcgg cctccgccac gacctgcccg tcgacgtacc cgacgccctt
     8341 cattttgcag gtgcggcccc ggaactggac catttccagc tcgaagcgga tctgatcgcc
     8401 gggcaggacg gggcgccgga acttcacgtt atcgagcgac atgaagtaga ccaccttgtc
     8461 ctcgacgttc tgcccctcga aatgccccat caggagcatc ccgcccacct gcgccatcgc
     8521 ttcgatgatg aggacgcccg gcatgatcgg gtggccgggg aagtgtccct ggaagaacgg
     8581 ctcattgatc gtgacgttct tgatgcccac gatccgccgg tctccctcca cctcgatgat
     8641 gcggtcgacg aggaggaatg gatagcggtg cggcagaatg tcgaggatcc gcccgatgtc
     8701 gtagcgggca tccccggtgc gtttggcggt ccgggaaagg gcccgcgcca gcgcgacgtt
     8761 gcctacgtgc gaaggccggg tcgcgacgat gtgcgcgcgg attcgggcgc ccgtcagcgc
     8821 gagatcgccc acgatgtccg ccgccttgtg ccggacgaat tcgtcgggcc agcgcagggg
     8881 ccccccctgc accaccgtct tctcgtccag cacgatggcg ttgtccttgt tcacgccctt
     8941 gatcagcccc ttggcccgga gagcgtccac ttcactggcg aagccgaagg tgcgcgcgtt
     9001 ggcgagctcc ttcgcgaagg cttccggcgt gatctcgtag gtgccggcct gccgcccgat
     9061 catcggatgc gcgaactcca ccgtggcggt gaggcggtag ctcttcgccg gcgccaccac
     9121 gtagctcgcg tccccctcgg tgaccgtgaa gggcgcctga acggtgagga ggaccggctc
     9181 gccgtcgtgg tcggccgttc ccgcttccat caatgccttg aagtagggcc cgacactgcc
     9241 gtcgagcgcc gggggctccg gtcccgacag ctcgatgatg cagtcgtcca cttcgagcgc
     9301 gccgagagcg gagaggaggt gttcgacggt atggatggtc gcgtcaccgt ggccgatcgc
     9361 ggtccgccgc tccagggcct ccacttcggt gaggcgggcg ggcacgagcg gcttgtcggc
     9421 gaggtcggtg cggcgaaagg cgatgccctg tcccgacgtg gccgggagca ggtgcgcctc
     9481 cgtgtgggca ccggtgtgca acccgacgcc cgagagcgtg acaggctgct tcagcgtacg
     9541 tctagcgatc ggaatcccct tccgggcgcg cgagcgcctc gagctcattg accagcggtg
     9601 ccaggcggta cagcgccgcc tgggcccgca gatagtcgcg atggttgcgg gccggatagc
     9661 ccgtgacggt cgcgccatct tcgatgtcgc cgatcacgcc gctttgcacc gtgatgcgga
     9721 cccgggaacc cacgttgatg tgatccgcga gccccacctg gccggcgagg aacacgtcgt
     9781 cccccacgcg cgtggagccg gcgattccga ccatcgccgc gatgaggcag cgctccccga
     9841 tccgcacgtt gtgggcgacc atgacgaggt tgtcgatctt ggtgccccgt cccacgatgg
     9901 tgtcgtccac gctgccgcgg tccacggtgg tgttggcacc gatctccacg tcgtctccga
     9961 tgacacagcg gcccacttgc ggaatcttcc gcggcagcgc gccgcccttg ccgggcagat
    10021 atccgaaccc gtcggcgccg aggcgggtgc cggagtggat catgacgcgg tccccgatga
    10081 cggtccccgt gtaacacgtg acttgaggat agaggtgcac gtcgttcccc acgatcacgc
    10141 cgtcccccag cacgcacccc gcgtcaatcc ggacgttctg tcccagccgc acgttctcac
    10201 cgaggaccac gtagggcccg atggcgaccg ggtcggtcca ggtggagccg cggccgatca
    10261 cggcggtggc gtccacgccc ggcacccacg ccggttcggg atagaggatc gggagcacgt
    10321 cgaggagagc ggcgtgcggg tccgcgacca cgatcctggt gggcggcccc tgcggaagat
    10381 cggcgaattc ggggcgacac aacaccaccg cagcctgcgc gcgctcaaag taggggaggt
    10441 aggaacggct cgcaagaaat gccaagtcgc ccgcgccggc ccgttcgagc ggggcgacgc
    10501 gggcgaccgt gacgtgtccg ggacccacga gccgcccccc cgtgagagcg gcgacgtcct
    10561 gcgcggagag cggccggtgg ccttccgctg gcaccgggcg cgctagttcg actgggccgg
    10621 cagctgtttg atgcgcgcga cgaccgtgcg ggtgatgtcc aacgcggggt ccgctgccag
    10681 gagccccgac gcactggggt caccattgaa caccatcgag tagccacctt cgagccgcac
    10741 cgcctcgatg atggtccgga tcttctgctg aatgggcgcc atcagctcct gctgccgctg
    10801 ctgggccttc tggttcagct cctgggtccg ggcatcgaag cgttgttgca tgttctgcag
    10861 atccttctgc ttcgcctgct tcgcggccgg cgagagcgcg atggactgct ggtccagggc
    10921 ctggaccgcg gagtccatct gctgctgcat cttctgcacc tcgtcccggt agccctgcac
    10981 ctcgcgggcc agcgtcgatt cggccgtcac gtacccgggg gtcgaatcga gaatctgctg
    11041 gggaatgacg taggcgatct tgagccccgt gaccgtcttg ggcgcggggg tctgcgcgtg
    11101 ggccgcttgc gcagaaaggg ttcgggtggc ggccaacgcc agacagacca ccaggacacg
    11161 cttcatgact gactccggag atggggacat aagagaatgg tctagaagaa gttgccgagt
    11221 ttgaagtgaa gtttccagcc gggtgcgggc tgccccgcca gattgacctt gtcgaagccg
    11281 tagcccaggt cgaggccgat cggccccagc ggcgacacca ccgccacccc cacgccggcg
    11341 ccccggaaca gccgggtcgg atcatactgc gcgggccgcg cccatacgtt gccggcgtcg
    11401 aagaacgtgc tgacgtagaa cgcctgcgaa agccggagcc cgatttccgc gttcatcagg
    11461 aagaacgcgt tgccgaacgc gttggcactc gcctgcccgc ccgccgcgct ggggtcgtat
    11521 cccttcggcg taacggagaa ctcgtcgtaa ccccgcagcg ggatcccgaa ctgcgtgccg
    11581 cccatcgtga acagctggtc gaagaacggc gacttcccga agacgaaccc cgagcgggtc
    11641 gacagcccca gcacgaactt cggcgggttg gccccggcgc ccttgccgcc aagggtcccc
    11701 aggggcgcat accagtgcga ctcgatgtcc acgcgctgga aattgccggt gccgcccagg
    11761 acgccgccgc tctgcgacac tccgatggac gtcatggtgc cgcccgtcgg gaacgggagc
    11821 tcaacgcggg tatcgcgcaa gatggacgtc ccgagggtcg accggaggca gttgctgcag
    11881 ccgaagctcg ttccgaacgc cgggttcaag gccgagccga tgaagctctg gtcgtcgagg
    11941 ctgtaggaca ggaagagccg gacgtgcttg cgggcgtcga agagcgggaa gccgaactgc
    12001 agcgatccgc cgcggcgccg gagctggccc aggtccgcga tggtgtagcg gtaccgggag
    12061 tcgtgcaggt cgagggttcc cgagatccgc gacagcatga tcgcggggtc ggtatacgag
    12121 atgtcgaagt cggtgatgtt ctggccgaac tgccactgga agtgcccgcg cttcccccgc
    12181 ccgaagagat tcggctcgtc caggccgatg aagccgccga ggccggtacc ctgacccacc
    12241 gacgcgccga aattcacgtt cccggtgcgc ttctcttcga cccggaagat gacgtccacg
    12301 tcaccctgat cgttggcggg ccgcgtgtcc ggaatgggaa gcggctgctg gaagaagcca
    12361 aggttggaaa tgttctgata ggaggcgagg agcgcgttct gccggaagac gtcgccggga
    12421 atcatctcga ttgcgtcgcg gatcaccgcc tcgtgagtga cgtcattgcc cacgatgtcg
    12481 atcttgttca cgatggccgg ctggtgctcc tggacgaacc agcgcaggtc caccaccggg
    12541 gtcccgtccc gttccacccg gcgcacgacg tcattctgga tctgtacgta gagatagccg
    12601 tcgtcgcggt aggccgtgta gaccttgttg gtcgcgtcgt cccactttgc ctgattgaag
    12661 taggcggcgc cgtgctccgt cccgcccagt cccaggagcc cggtccggcg gctgctcgag
    12721 aacggataca ggccctggat ttcctcggtc gtgaagtgcc ggtttccggc gatttcgaag
    12781 gtgccgatct tgtacggctg accctcgctg aggcggacca ccaaggtcgc cttgcctgtg
    12841 gtgtcgtcga cgatgagggt gtcaccgagg acctggaagt ccacgtaccc gcgggctccg
    12901 tagaaatccg ggagcttctg ggtcaggtcc ttctgcagct tgtcttcgtc gtactcgccg
    12961 ttgcgccaga agaagaagcc ctcgggcttc gaactcatct gatcgacgag tgtctgcgcg
    13021 gtgaacgcat gatttccctc gaagttcacc cgggcgatgg cgacgcggcg cccttcgtcg
    13081 atctcgaaca ccacgcggac ccgcgaggag tccgcctcgt agatccactt cgggtcgacg
    13141 gacgcgaggt aatagccgtc accccgatag agcgaatcga tccgggcggc ggaccgggcg
    13201 acggcggcgg ggtcgagggg ccgtccctca aacagagtca cccgctcgcg gaccgtgcgc
    13261 tcggaaatct tctcggtccc cttgaccgtc cacttgatga gaatgggccg ctctttgacg
    13321 gtgaccagca gaatggagcg gccggcggag gtgtcggcgg cgacgcggac gtcctggaac
    13381 tggccggacg catagagcgc ctcgagggcg tgctggacgt cgcggtagcc cgtgacccgc
    13441 ccgacggcga agccggacgt ggagaggacg gtttggcggg tgacccgctt gatcccgagg
    13501 accgcgatgg agtcgggaat gatctgcgcc gcctgctgcc cccgcgcgat ctgggggagc
    13561 aggcagagga gaccgcaaac ggcgccgagg gagacccggc ggatcacgcc ttcgagccgg
    13621 agagggcgcg gaatgcgagg cgctccttgt cgggcgccac gtcgacttcg atttcgtctc
    13681 cccgcgcgaa ctcgccgacc agaattttct ccgagagcgg atcttcgacg aagcgctgaa
    13741 tggcccgctt caggggccgg gcgccgtagt gctcatcata cccgtgatcg accaggaagt
    13801 tggaggcggc ctcggtgagg cggagcgtga gctcctcgtc gccaagccgc ttctgcacgt
    13861 ccttcagcag gatcaccacg atctgggcga tgtgctggcg gttgagcggg tggaacacaa
    13921 tgacgtcgtc gagccggttg aggaattccg gattgaagac cttgttgatc tcctccttca
    13981 ccttctcagc catccgggcg aacgaggcct cgccatcggg ctgcgcgaac cccatcgtct
    14041 tgcccttgac catgtcccgg gcgccgacgt tcgaggtcat gatcacgacg gtgttcttga
    14101 agtcgatcac ccggccgtag ttgtcggtca ggtggccctc atccaggacc tggaggagga
    14161 tgttgaagac gtcggggtgc gccttttcga tctcgtcgag gagtaccacc gcgtacggct
    14221 tgcggcggac ggccttggtg agcgtgcccg agtcttcgta acccacgtat cccggcgggg
    14281 cgccgatgag gcggctcacc gagaatttct ccatgtactc ggacatgtcg acgcggatca
    14341 gggcgctggc gtcggcgaac aggaacttgg ccagggcccg ggcgagctcc gtcttcccca
    14401 ccccggtggg gccggagaag atgaaggagc cgatgggccg cttcggatcc ttgagcccgg
    14461 cgcgcgaacg gcggatggcg cgggcgatga cctcgatggc ctcgttctgt cccaccacgc
    14521 tggcgtggac ttcgtcttcc atccggagga gccgggccgt ctccgcttcc tggaggcggg
    14581 tgaccggaat gcccgtccac cgggagacga tgaaggcgat gccctcttcg tcgatcgtgg
    14641 gacggcgggt ctgccgctcc ttctcccact cctcctgctt gcggcggatc tccatctgga
    14701 gctcgcgctc ctgatcgcgg agactcgcgg cccgctcgaa gttctggtcg cggacggcgc
    14761 cctctttttc ggtgttgatc ttctcgagac gctccttgag cgccgccgct tccgggggcg
    14821 gcacctgcgt cgcgagccgg gcgcgtgcgc cggcttcgtc gatcacgtcg atcgccttgt
    14881 ccggcaggaa ccggtcggtg atgtagcgct ccgagagccg cgccgcggag gcgagggtgt
    14941 cgtcgggaat gatgacccgg tgatggtcct cgtaccgttt ccgcaggccc ttgagaatct
    15001 cgatcgtctc gtccacggtg ggggggtcga cgatcaccgt ctggaagcgg cgctccagcg
    15061 caccgtcctt ctcgatgtac ttgcggtact cgttcagggt cgacgcgccg acgcactgca
    15121 gctcgccccg ggccagcgcg ggcttcagca tgttcgaggc gtccaccgct ccttcggccg
    15181 cgccagcgcc cacgagcgtg tgcagctcgt cgatgaacag aatgatgttc ttgttctgcg
    15241 cgatctcgtt gatgaccgcc ttgaggcgct cttcgaactg gccgcggtac ttcgtcccgg
    15301 cgatgacggc ggccatgtcc agcgagagga cgcggtggtc cttcagcgcg tccgggcact
    15361 ggccaccggc gatgagctgg gccagccctt cgacgatggc ggtcttgccc acgcccggct
    15421 ccccgatgag cacggggttg ttcttcttgc ggcgggacag aatctccatc acccgctcga
    15481 tttccttctg gcggccgatg gtcgggtcga gcgcgccctc gttggcgagc tgggtgaggt
    15541 cccggcagaa atgatcgagg gccggcgtct tcgatttctt ctcgctcttg gggctggcgg
    15601 gctgcccggc gggcgggccc ccggccggcg ccgcgggcat ttcactgccc aggagccgga
    15661 gggtttcggc tttggcctgc tcgaggttca cgcccgcgtc ggtgaggacc tgcgccgcga
    15721 tgcccttctc ttcgcgcaga aggcccagca ggaggtgctc ggtgccgacg taggagtgat
    15781 tgaggtcgcg ggcttccgcc atcgcgagct cgagcacctt cttggcgcgc gacgtgtacg
    15841 gcaggtcggg gccggccgcc gcggcggctt tccccttctt gaccgtctct tcgattttct
    15901 gctggatctc gtccagatcc acgttcaggt tcgtcagcac cgccgccgcg accccctcgc
    15961 cctcgcgaat gaggcccagc aggatgtgct cggtcccgac gtactcgtgg tgcagacgcg
    16021 cagcttcctc acgcgccatc tgcaggacct tccgcacccg atccgtgaag ttgtaaccgt
    16081 tcatcgcctc atcaccctca gggggattcg gattccagca agcgccggac gtatgtcgca
    16141 cgcaccgtcg gcagctcagg atcgccaccg gtccgaccct cgagcgccgc caggtgcgcg
    16201 ggctgcgtat aaatcaggag cttgttgagc gtgtatacac tcaagcccgg tatcaggtcg
    16261 agcccgacac ccagccgtac cccgctcagc aaattcatcg cttcctcgaa cgtcagccgc
    16321 cgcgcgtgcc ggagcagtcc atacgcccgc cacgtcttgt cggccacttc atccggcgcg
    16381 gtccggagca gcatctgccg ggcctggtct tcgtactcga tcacctgccg gacgatcttg
    16441 ccgaggtggt cgagcaactc gccttccgac ttccccagcg tggtctgatt cgacaactgg
    16501 aagaagttgc cgacgacctc actgccttcc ccgtagaggc cccgaaaggt caatcccacc
    16561 tgggccaacc cctgcagcac ctttccgatt tccttggtca aaacaaggcc cggcaagtgg
    16621 ataagcacac tggcgcggag cccggtgccg gcattggtgg ggcaagaggt aagatagcca
    16681 aactccgggt gaaacgcgaa gggaagcagc cgcccgaggt ccgcatctat cgattccagt
    16741 tgagcataag cctcatccag cgcgaaccct gaccacatcc cctggagccg gaggtggtcc
    16801 tcttcattga ccatgacgcc gaccggcgac tgggcgacca gggcagcccc cggccggggc
    16861 cgggtatcct ggtcgagacc cgccaattcc ttgctcacca ggtgccgctc gtgcaggagc
    16921 tgccggtccc cccgctccaa agcgtcgagc cggaacgaaa cgttccgttt caggcgctcg
    16981 ctggccgccg ccgcctcgag cacccggtcc aggaccgcca ggcgctcgtc atcggtggac
    17041 cgctgtgaga acgtgtgccc cgccaggttt ctggccaggc ggatgcgcgt ggagaggacg
    17101 atctggctct tggggcccga cgcatcgagc cagcccaggc cgccatcggg gaggagtgac
    17161 aggtccatca ttcgagtacc cggatctggt cacgaagctc tgcggccagc tcaaagttct
    17221 cggcatccac ggcctggcgg agctggtgcc ggagctccag cagccggtgc gcgggatcgg
    17281 catcctcgcc gccaggctgc tggtaccgct cgcccacgtg ctgactggag ccgtggagcc
    17341 ggcgcaggag gtctcgcaag tgaacctcga acgccgcata gcactgtgcg cagccaagtt
    17401 tgccggtttc ccggaagtcc ttgagcgtcc cgccgcagtt ggggcacggc gtgccatcga
    17461 cggtcgcggg caggagcgac cccgcggtct tccccatcgc catgatgaac ccgcccaagg
    17521 gcgacttcaa cgccgcggcc cccgtttcca cccccttctc cgctgcgcat tgctcgcaga
    17581 gatggagggt gactttcgag tcgtgctcca cctgggtcag gttgatgacg gcgtcccgtt
    17641 ccttgcagtg gtcgcagagc atcaggggat cgcctccacc gagggcacgg cgcccaggcg
    17701 gccatcttcc agccacagga tgcggtcggc acgcgcggca agctgccggt tgtgcgtcac
    17761 gaccacgacg gccgtttcga aatcccgcgc caggcggaag aacgtctcgt gcaggacctc
    17821 ggcgttggca tggtccaggt tcccggacgg ctcgtcggcg aggacgagcg cggggtcatg
    17881 gaccagggcc cgggccaccg cgcagcgttg ctgctcccct cccgacagct cggcggggcg
    17941 atgcgtcatc cggcccgcca gcccgaccgc cgagagccgc tcttcggcgc gggaacgcgc
    18001 ctgccgctcg gaggcgccgc cgatgagcat cggcatcatc acgttctcga gggcggaaaa
    18061 ctcccggagg agatgatgga actggaagac gaacccgatg cgccggttgc ggagctcggc
    18121 cagtgcgttg gcgtccaggt cggcgaagcg ggagccctcg agccagatgt caccggcggt
    18181 gggccggtcg agggcgccca tcaggtgcag gagggtgctc ttccccgcac cggaggtgcc
    18241 cacgatcgcg atcagctccc cccggcgcac gtcgatatcg acgccgcgca ggacctcgag
    18301 cggctgtcca tcgccaccgg cgaagacctt gcgcagtccc cgtgactcga ggaggccgtt
    18361 cattcgtacc ggatcgcggt gacgggttcg aggtcaccgg cgcggaaggc cggaaagagc
    18421 ggagcaatcg tcgccacgag gagactcgcg gcgatgacgg ccagaatgtc gagggcctgg
    18481 aaatggaccg gcaagtggtc cacgaagtag atcgacgggt cgatggggat ccagcgtccc
    18541 tggttgacca tcacgccgac gatgataccc gtcaccgctc ccagcgcggt gccggtcagc
    18601 ccgatgatga tgccctgcgc cagaaatacg cgccggatgc tggcgcgccg cagccccatc
    18661 gtcaggagga tcccgatttc ccgcgtcttg tctcgtacca ccatcgtcag catcccgacc
    18721 acgttgaagg cagcgaccac acagataagg aacaccacca gccccatggt gagtttctcg
    18781 agctgtaaag cggagaacag ggcgccgttc tgggtctgcc aatccatggt gcggtacggg
    18841 taccccagat gggtttcgag cgccgcgccg aaggtgcggg cgtgcccggg atcggcgagc
    18901 tgcacctcga taccggtcac cgcggtgtcg agcccggcca ggcgctgcgc cgcggagcgg
    18961 ctcagcacga cgtaactgtc gtcgtagatg tacatcccgg tgtcgaagat gccggtgacc
    19021 tcgtaccggt gatactgcgg cacgaaggta ccggcggagg cactgaagct cgaccgcccg
    19081 gtgaacgaca ccagggttac gacgctgccc acgtacgccg agatcttccc ggcgaggcga
    19141 gttcccagcg cgacccctcc ctcgacgtcg gggcgcgtgg tgcggaagct cagatcgccc
    19201 ttgatgaagt gttgcgcgaa cgtggtgacg gcccgcgtgc cagtgtcggg ctcgatgccg
    19261 tacaccacca cgccctcggc gtagtcgtgg ccggtggtga cgcccgcctg ggtcatgacg
    19321 aagggtgccg cggcggtcac ccccttggtg gcgcggaccg aatcgaggac gcggcgccag
    19381 tcgtccagcc ggagtccctc gccgtacgtg agcacccgca ggtgcggact tccgaccagg
    19441 atcttgtcgc gcaggtccga ctggagaccg ttcatcacgc cgaggacgag catgagcgcg
    19501 gcgacgccga cgaagacgcc cccgacggcg atcacggtaa tgaacgacag gagccccgac
    19561 gaacgccggc tcgtcaggta ccgcagggcg atgcggagct ccaaagcgcc gatcattccg
    19621 gcctgagcat cggaaagagg atcacgtccc ggatgttggt ccggtccgtg aggagcatga
    19681 cgagccggtc gatgccgagg ccgacgccgc cggtgggggg catgccgtac tcgagggcgc
    19741 ggaggtagtc ctcgtcgatc tgatgggctt cgctatcgcc ccgctcccga aaccggactt
    19801 gttcctcgaa gcggcgcctt tgctcgtcgg gatcgttgag ctcggagaag gcgttcgcga
    19861 tttcgcgccc ctggatgaac agctcccacc gctccacgcg atcctcacgg tcccgcttgc
    19921 gcttcgcgag cggcgacagg gcgacggggt gattcatgac gaacgtcggc tggacgagcg
    19981 tcggctcggc gaaggtcttg aagacctcgt ccatgagctt ggcgcccgag tactgatcgg
    20041 cgtcggtgac ccctttgcgc ttcagcgcgc cgcggagggt ggcctcgtcc gccgcgccca
    20101 agtctacccc ggccgtttcc ttgaccaggt cgtagaagtc ccgccgggcg aacgggcgtt
    20161 tgaaggacag cgtcgccccg ccccgctcga gcgtctcctc gccggtcacc tcgacggccg
    20221 cgccggcgac catcgcctcc gtcagattca gcatgtcctc gtagtcggcg tacgcctcgt
    20281 accactcggc catggtgaac tccggattgt gccaccggtc cacgccctcg ttccggaaat
    20341 cgtgcccgat ttcatacacc ttctcgaggc cgccgacgat gcagcgcttc agatagagct
    20401 cgtcggcgat gcgcagatag aacgtcgcgc ccaaggcctc ataaaacgtg gtgaagggcg
    20461 tcgcggccgc gcccccgtag agcggctgca ataccggtgt ttccacctcg agatagccgc
    20521 gaccgtccag gaaccggcgg acgtagctca ccactcgcgc gcgcgcatga aacacggccc
    20581 gggcctcggg gtgcgccgcg agatccgcgt accgctgacg ggcgcggagc tcgggatccg
    20641 tgagctcgcc atgccggacg ccctgcgcgt cctccttgcc caagggcagc ggacggagcg
    20701 atttcgcgag gagggtgagc tcctcgacgc ggagcgtgac ttcgccggtc ttcgtcttga
    20761 agagcgggcc ggcgacgccg accacgtccc ccaagtcgag cagcttgatg atctcgtaac
    20821 gttcgcccag gaggtccgac ttgaaataga gctggatctt gccggacgcg tcttcgagat
    20881 gcgcgaacgt cgtcttgccc atgccacgca tgaggacgag ccggccggcg agccggtgcc
    20941 gggtctcgtc cccgtccgtg aatccctgca gcgctggctg ggccagcccg gtccggtcga
    21001 atcggtaggc gaacgccggc acgcctttcc gggcgagctc ttcgagcttc tcgcggcgcg
    21061 cggcctcgac cgaggtcgtc atgcggcttt accggcctgc ttcttcagga acgcctcgat
    21121 gaactggtcc aggtctccgt ccatcacctt ctgcacgtcg ccgaccttca gctcggtgcg
    21181 gtgatcgttc accatggtat agggctggaa gacatacgag cgaatctggc ttccaaacgc
    21241 gatgtccccc ttctgcttgt ctacttccgc cttcttggct tcctgctcct ccagcgcttt
    21301 ttcatagagc cgggacttca gcatcttgag cgcggtcgcc ttgttcttga actggctgcg
    21361 ctcgttctgg cacgcgacga cgatccccga cggaaggtga gtgatccgca ccgcggaact
    21421 agtcttgttg acgtgctggc cccccttccc cgacgctcgg aacacgtcga tgcgaagatc
    21481 gtcttccttg atctcgattt cgatgtcgtc atccacgacg ggatagacga aaaccgaagc
    21541 aaaggaggtg tgacggcggg cctgcgcatc gaagggggat atccgcacca gccgatgcac
    21601 acccttctcg gcccgcagga ggccgaaggc gtactgcccg cggatctcga tttccgcgga
    21661 cttgatcccg gcttcttctc cctcgagcag gtccaggacc tcgatgtcga acccgcgccg
    21721 ttcggcccag cgcgtgtaca tgcgcatcag catctcggcc caatcctggg actcggtgcc
    21781 gccggcgccg ggatggatgg tgagcagggc gtctcgagga tcgtcggggc cccgcagcat
    21841 gtgctggagc tcgatcgcgt tcagagcccg ggctgcggcg tcggcttccg cctcgagcga
    21901 gcgctgcatc tccagatcga cgttcgactc ggcctcgaga agctgtgaca gctcgaagcc
    21961 gtcatccacc cgtttcttca gggcgtggaa tggctcgagc tgccgcttca gcgtcttgac
    22021 ctcttctacg accttcttgg cgcgctcctg gtcggaccag atggcgcctt cggccatcgc
    22081 gtgctccagc tcggccaggc gcttcgtttt gccgtcgacg tcaaagaaag ctccggagct
    22141 ccgccagacg ctcctggaat tcggaaaatc ggttcgccaa gggcacgggt gtctcgctcg
    22201 atctcaatag acgtaagccg ctccccggat gtggggaacg gctctaactg acaggaatat
    22261 gaggagttag accgcgaaat tccagcggtt tagaagacct tctgtccccc ggcgagcagc
    22321 tcgttcaggg cgtcggtcca gaacggcgtc gatgacgcca tttcctgccc cacctgctcc
    22381 acgtactcga gccagctctt ctcgatctcg tctttgaaga gttgaggcaa cgtgccgtcg
    22441 cgagttccct gcatgcgctt gtccgggtgg tacaccagca tgtccgagat caacgcgcgc
    22501 gcgagacgac gggctttctg ttttgggtcc tgcaccatga acggattcac cggacgcaga
    22561 ggtccggtct tccggagctc ggcgcctccg gctggtgcgg cagtcgcgac gggtggagcc
    22621 gtggcggccg gcatggcggg cgctggcggg gccggcggag atgccggcat tgcgggtgcc
    22681 gcggggggcg gcgcggcggg acgcgccggt gcggccggca tcgcgggagc cgcgggcatg
    22741 cgcggcgcgg tcgcgggagg agcgaccatc ggtgacctcg ccgggggcgc catcatcgga
    22801 ggcgcaactg gcgggcgaac gggggccgcc ggcggaccga ccgacggacg tggaggcatc
    22861 gcgaacgccg gtggctgcgc caccggaggt gcgggcacga ccggtggcgg cgggggcgcg
    22921 ggcgtgaaca ccggagcggc cggcggcggg agcggtggtg ccggggccgc catcggagcg
    22981 ggtgccggag ttgccggagg tgccggagga ggcgagggtg gcggtggagg cgccgcggcc
    23041 gtgacggcag ccggcgcagg ggccgggacc gccaccggct gggttccgtt ggccgaaacc
    23101 gggaagacgt tggagcagac cgagcaccgc gcgcgaatgc ctccggccgg gaccttggcc
    23161 gggtctaccc ggtagatggt ttcgcacgac ggacaggtga cgttcacggg gctcccgggc
    23221 tcacttccct attcaggcgt ttcaccggac tcgtgatcgc gacggcggtc caccacgaac
    23281 actgacccgg cctgcgtctt cgcgtaactc ttcatctccg tcgccagctc cgacacctgc
    23341 gcgggatggg cgaaccggcg atgctggttg gtcacgatcc cgatggacag cgtcatcagc
    23401 ggcacccggt gcaactgccc ccggcgatcc ttcccgaaat agtaccccgc gcggcggtcc
    23461 tgctcgttgt actgatacgg gatgagcgta tcgaacaccg tgaggatctc gccgcacacc
    23521 tcggtaatcg cgtcgacggg gatcatgaag atgaagtcgt cgccgccgat gtgcccgatg
    23581 aatcctcgtg ggcccgtcat gcccttcacg acgtcatgca agatccgcga cagcaagtag
    23641 atgacgcggt cgccgtcgta atagctgtac cggtcgttgt attccttgaa gtgatccagg
    23701 tcagcgtagc agaccgcaaa ccactccccc gcctccatcc gccggcggat ctcccgttcg
    23761 atctccgtcg tgcccggcaa gcgcgtcgac ggattcacgg cgacatcgcg cgcggtgcga
    23821 ttcaggagcc cgtcgagccg gacctcctgt tccgccggtt ccagcagcgg cgtgaggacc
    23881 tcgtcggctc cgttcgcgaa ccactcgcgc accttctccg tcacgtgccg cccggcgacc
    23941 gccgccagcg ggacgatggc tgtgtatgga tctcccttga gcctggaaca caactcgagg
    24001 gaggcggcac cgtccgagtc cgcgtccacc agcacgagga ggggcaacgc ccgcaggacc
    24061 atctgctgga tcaccgcagg gtccgagacc tgcaccagac ccgtctgttt cccggtgatc
    24121 cacgggagaa gggtatcggg tacggggcgg ccagtcgggg aaaagaagag aatcgtctgg
    24181 cgcgtccgca ccgggctcct cgcgtaaaag tcgagcgcgt cgaaacttag gcccgctccg
    24241 gggcggtgtc aaaccacgac cgtctagaac gccacgtgga cggcaaggag ggagaaatca
    24301 ggtcctatga cggtgtgcag gctcgtggtc cggtgggttg cggtggtcag ccggttggag
    24361 atgagcgcgt cgagggcgct cacgacgtgg ttcgccagga ggagcccgag ggcaccttgg
    24421 gcgttccgat acccgttgtc cgaggttcgg atcaggtccc ggaaggtctg ttgctggaga
    24481 gaggcgctct gccacgtcca ctggtacgcg ggtccgacgg cgtgctgcac gtagaacgac
    24541 agcgcggcct ggtattgggg actgttggga ggcggcggaa cgttggggtc ggcccagtag
    24601 gtacggcggg ccaggagcca aacggatccg ttgtacgtcg tgggatcggt ctcgggattc
    24661 aggccggggc cggcgccggt gttgaaccgg cccgacgccg tgaatttttc catcgtttcg
    24721 tagtattcga agatcgtatc gcgccgcacg gaggcgaatc ctcgctgggc caccgcgaac
    24781 gccagctggt gatagcgctc ggcattcagg ttcgcgtcgt gctgcagctg gatgtagcgc
    24841 gacaggacgt agatctcgac cgcaccgtac accgccgcgc ggtcctgtcc tgaaagcgcc
    24901 tgccccgtcc ccggcagaac gagggacgcg agcggccgca cccaggactt ctcccggagg
    24961 gcgaccgaat cgacctgcgc ccggaggggc ggggccaggg ccgatccgat gaaaagggtc
    25021 agcgcgagcc accgccggtg catcagaagg tcaggctgaa ggaaatgaag gtcggattcg
    25081 cgcccagcag gtcggtgttc tgtacgaacg tgcgcgcgaa gtcgatcgcg atggatccgg
    25141 tgtggacccc catcccgatg ctgggtccgg acaatccctg ttgcgcgaag gcgtagcccg
    25201 accggactcg gatggtttcg tgatacccta cgtcgatacc gaaccgcatt tccggtggcg
    25261 cggaattgtc ccaggggcga tcgaggtcag cggcaagccg gatatccacc tggttgagtg
    25321 ccgacgacag gtcgagcctt cccgaatcgg gagcggcgag cgtcggcgcc cctccctggt
    25381 cggcgggatg caaatcgatc cggtagagcg cgccgagcga gaggcgcgcc gggaggggat
    25441 cggactgggc cgcgttgttc acctggagct tgaagccgat gtccttcacg gccgcgccga
    25501 atcgcagggc tcctcccgat ccgaccgcga cctgggtgcc gacgtcgaag gcatgcgtca
    25561 gcgcgtcgct gccgttcggg aggcccgcgc aatttccctg gcagtcgatg gtgaactgaa
    25621 cgagcttgta cgtcactccc accgagacgg cgcccgcgag agaggtcgcg aacgacgcga
    25681 ggtactccac gttcctgggg aagatcttcg cgatcgcgct caggctgtcg ctcccgacat
    25741 cctgttcgcc gtagtcgagg agaaatacac ccacgccgaa ggtgccgatg ccccggcgcg
    25801 gtacgaacat cgtgatggcg gtgccgggcc cggccacgaa gttcgcgctg gagaccgcga
    25861 actcggccgc cggcaagctt gcgaggccgg cggggttcca gaagacggac tcgccgcggc
    25921 cctggagggt tacccctgcc tgccccatgc ccacgctctg tgcaccgatg ggcagcagca
    25981 tgaagagcgc cccggcattc gtgagttggg gcgaagacgt gtccgcctga gccgcgagag
    26041 ccgaccaacc gccagtggcc aggagcgcgg cgacagcgag ggcccctcta gatcggcttc
    26101 gcttcatcca ccagcatgac cgggatgtcg tcttcgacgg ggtacgacaa ccggcacgcg
    26161 ggacacgtca gcgtttccgc gggcgcggtc cggtgctcga ggggaccctt gcacttcgga
    26221 cagaccagga tggcgagcag gtcgggagag aggctcatcc tatggagtac cccaggcgct
    26281 tgagggaaga tgcatccgcg cgccaccgcg gcatcacctt cacgtgaaga tcgaggaaga
    26341 ccggttgacc ggtgagggcc tcgatgcggg cccgggcctt ctggccgatg gctttgatca
    26401 ccttcccgcc ggccccgatc accatgccct tctgactgtc ccgttccacg tagaccgttg
    26461 cccgaatata taccggcgtt tccgactccc ggaattcgtc tacgtcacag gccaggctgt
    26521 agggcagctc ctcccgcaac tcgccgaaag cggcctcgcg cacgtattcc gcggcgaaga
    26581 accggagggg ctgggtagcg aggtcatcgt caggataaag gaaggggctt tcgggcaggt
    26641 gggcgccgag ggcgtcgcga agggcggcca cgccggcgcc gctggtggcg gagatcgcca
    26701 gtccgcccag gggcgtcgcg accgtcgggc gcaggtcgca cttcgtgtac accgtgacga
    26761 cgggcgcgcg cggctgcttg gggaggccgg ccacctcgac gagtggcggc gcgtccccgt
    26821 ccccgagcgg atggagatag gcgatgacgt ccgcgtcctc gagggtggcg agcgccgcag
    26881 cgcgcatcac ttcctgcagc cggtattcgg gatcgaggag gccgggcgtg tcggtgaaga
    26941 tgaactgcgt ctcggcggtc gtgacgaggc cgacgacggg gagacgggtc gactggggtt
    27001 tgggtgagac gatggccagg tgctggccga cgaaggcatt caggagggtc gacttgccca
    27061 cgttgggccg gccgaccagg gccacgactc cgcatcgcgt catggagaga aggtagcgga
    27121 aaaacgaagc gccgagcttg cgctcggcgc ttcgaaggat tggctggcgg cggcctactc
    27181 tcccacggtc tcgcaaccgc agtaccatcg gcgctgacag gcttaacgac cgtgttcggg
    27241 atgggaacgg gtgtggccct gtcgcgctag tcaccagcca tagactgcat gacatccgac
    27301 agccgcgtgt gtgttgttca aagaaactca ccgagaaact tgggaagaga tcaagcctca
    27361 cgggcaatta gtacggctcg gctgaagcgc tcgcgcgcct tgcacctgcc gcctatcaac
    27421 gtggtagtct cccacggccc tttaggggtc ttgcgacccg ggagtgttca tcttggggag
    27481 ggtttcccac ttagatgcat tcagcggtta tccccgcgcg acatcgctaa ccggcgttgc
    27541 tcctggcgga acagccggga cacgagaggt cgcttcgtcc cggtcctctc gtactaggga
    27601 cgacatccct caacactcca acgcccgcac cggatacaga ccgaactgtc tcgcgacgtt
    27661 ctgaacccag ctcatgtgcc actttaaccg gcgaacagcc ggacccttgg gaccttctcc
    27721 agccccagga tgtgacaagc cgacatcgag gtgccaaacc gcgccgtcga tgtgaactct
    27781 cgggcgcgat aagcctgtta tccccagcgt accttttatc cg
//
LOCUS       DASUBP010000170         5784 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1054340, whole genome shotgun sequence.
ACCESSION   DASUBP010000170 DASUBP010000000
VERSION     DASUBP010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5784)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1054340"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..1446
                     /locus_tag="VFU75_06425"
     CDS             241..1446
                     /locus_tag="VFU75_06425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466506.1"
                     /translation="MADPKNRPPLRLLALLLGLAACEHAQPFEAPPVPTVTGPLSPGN
                     PTRLTYNPGEDGLPVWAPDGSYLLYQAENLNRSDKDRCLFLIPPTGGTQTLEACDDAL
                     HSEDSTNVFGPAAVSSTGRVILYEGAEPIGLGAQTSAGMYLAPRLPLMSATELLPLPL
                     VIPGGALADKLMSLQWVGDSGFLSLGVQTQVYIPCFLCTPDTLETGVGVVWTRLGNGS
                     PAVSAVPGTARASSYAFVSPDTVYFTLNGDPRILRTRVGGGPVDSVFAFPAESTDAPV
                     GFRTASPAIARGVQVLGGVLYAITGGRIEYHPDSGSGEFQRDWGGKLHKVALATGADS
                     LVSVSSGNPQKSVSSVWLHNPTLSSTGSLTAEGRPVTIVMLYSPTNQFIGTDTIIPRS
                     TNLWQYGHP"
     gene            1433..2458
                     /locus_tag="VFU75_06430"
     CDS             1433..2458
                     /locus_tag="VFU75_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466507.1"
                     /translation="MAIHSVVRRVAILGALIGALSCEHTQPFSNPGYESTTPLQPGNP
                     AQLTYNPGADVRPSWLPDGSAFLYTQEQTDITERDRCLVVMPKTGGGITRTICAADDF
                     AHDTLNDLESAAVNTAARMAYVRTSMLAGIGRAGPDHAAMVLGSYGTPVPGTVLHVLP
                     YPSPSGQGVDMASDLSWVGPSALVYLGEQVTYPAVCAGCVTVDTVRSGVEIDRIDLNG
                     TISVVVDTGFPTSASVVGGDTLYYTLTNSGTIHRRILSTAADTVVFDYGLPATDVSAV
                     RGRLAVVVLGQLHVLSLSPFADQTLTTPHPGQIIAHPALDPAGHLIVAEVSDSTALSN
                     LWLWTVP"
     gene            2455..5229
                     /locus_tag="VFU75_06435"
     CDS             2455..5229
                     /locus_tag="VFU75_06435"
                     /inference="COORDINATES: protein motif:HMM:NF026255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5466508.1"
                     /translation="MNRTWLVLSLAIAAPLAAQSTGAIAGRVTDDSTKHAIGGALIWV
                     DGGRQGATTDTGGAYRVRDVRAGWHRVRAARIGYRSVILDSVFVRSGETATLDFALAP
                     QALTIDSIVVRGEVDQVLNPLATADVQAITGNEIRRLPVTTVAEAVALSAGTVGESYR
                     GGRIGESAFVLDGLGVKNQLDASTNTLGIQVPTEILTEASLVTNAFSARYGQALSGII
                     NVVTRDAGDHWSGRAAYEGDRPMPQADDHGLDRAVFTADGPLGRGIGFVGVLNAQGQI
                     DGDPVSAPAPTDPHDPRTAEPWILPHNGAERLDAAGKLIIPVTQQNTIRLFGLRSAAQ
                     QYLFDPAFKYDETFAPATRTVGNLYSAHWQHTAAPQAVHPFGADVRVGYFNQDFIRGQ
                     TTNTPGDLVGAFSGQSLHFLDEGLARAKDTVAAMNALPGYTPPALSANSPWGVPAFFL
                     SNGSRGELAWNRYRELRFQGDFTLAAGPTTDFNFGGEFEQQHVETFQRVDAYVPVAAV
                     KDSVPPPTASDFRPQSAALYGETQMQIEDIALTIGLRYDQFDPRNTTVGQRFGARRTI
                     SPRFAMSTVLHGATFVASWGHFAQAPDFQYLVNSAFDDTTRTGRFRQGNPDLGFETAT
                     QYEFSVRGRPSEQTSLRVNAFVKRLDGLVASVPLSVNPDSAIFGNTDFGTVKGVELIL
                     ERELRDWWGARISYTLQDAQATSTNPFLLLNNFTIDQQGDTIRSARVQFPLDYDRRHA
                     LTVVLQARVPDDLGPRIFGSRVFGGVEAAVIGRYDSGLPYSKTNLAGDSILGLPNSQR
                     LPSQTQIDMLLRRSISLLGWRGGVYLDVRNLLNAANIEAVRRDVGSPFPSSGELQTMA
                     TNAYNANPQPIPYESPRYRAWADLNHDGYISGQAELMPLYLAAAKDFTQPIFQFGPPR
                     LFRLGVELEF"
     gene            complement(5226..>5784)
                     /locus_tag="VFU75_06440"
     CDS             complement(5226..>5784)
                     /locus_tag="VFU75_06440"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU5466509.1"
                     /translation="GEALTPLRMFLAVLVMIGVGLTVSGGDVGAVLSGGPGFKVGVLG
                     GLIAMFSYAGTTLLARWSVPRYGSIRSLFLELAGGVVMMAIFLTLFHRTPFTYSARPE
                     GLAAWAFCLAQAVGPVVGANFLFFAGVRRIDAAPTAVAATVEPVVGTLLALILFSQHL
                     SALGWLGLLLVVGGVATSYRSEARD"
BASE COUNT          963 a         1972 c         1878 g          971 t
ORIGIN      
        1 gtccgagggc taccaggcgt tcacggccct gaagcttcac aacgtccccg gcaaattcct
       61 gtacttcccc gatgaggggc acttcgtggt gcggccccgg aatcggcgtc tgtggtgggg
      121 agtcgtgctc gactggatgg atcaatacct gaagaactga ggacattgcg tgcgcgcgac
      181 gtgaacccgc acaaagaggg cgggtatgca atccgacccc acgactggag tggttcgagc
      241 ttggctgacc cgaagaaccg ccctccgctc cggctcctgg ccttgctctt gggccttgcc
      301 gcgtgcgagc acgcgcaacc gtttgaagca ccgccggttc cgaccgtcac gggaccgctc
      361 agcccgggca atcccactcg gctgacctac aaccccggcg aagatggcct tccggtctgg
      421 gcgccggacg ggagttacct cctctatcag gctgagaatc tgaaccggtc cgacaaggac
      481 cgctgtctct tccttattcc cccgaccggt ggcacccaaa cactggaggc gtgcgacgac
      541 gcgctgcatt cggaagattc gaccaacgtg ttcggacccg ctgccgtctc gtcgaccgga
      601 cgggtcatcc tgtacgaggg cgcggaaccg attggcttgg gtgcccagac cagcgccggg
      661 atgtacctcg cgccgcgtct tccactgatg agtgccaccg agctgctgcc cctgcccctg
      721 gtgataccgg ggggcgctct ggccgacaag ctgatgtcgc tacaatgggt cggagattcc
      781 ggcttcttgt cgctcggcgt acaaacgcag gtctacatcc cgtgcttcct gtgcaccccg
      841 gatacgctcg agaccggcgt tggggtcgtc tggacgaggt tgggcaatgg cagccccgcg
      901 gtctccgccg tcccggggac cgcccgggca tcgtcgtacg cgttcgtctc gcctgacacg
      961 gtctacttca cattgaacgg cgaccctcgg atcctccgga cgcgcgtcgg cggcggcccg
     1021 gtggactcgg tgttcgcctt tccggcggag tcgacagatg cgccggtcgg attccgtacc
     1081 gcctcacccg cgatcgcgcg cggcgttcag gtgctgggcg gtgtgctcta tgcgatcacg
     1141 ggtggacgga tcgagtatca ccccgactcc ggcagcgggg agttccagcg ggattggggg
     1201 gggaagctgc acaaggtcgc tctcgcgacg ggtgctgatt cgctggtgtc cgtgtcgagt
     1261 ggaaacccgc agaaatcggt gagctccgtg tggctgcaca acccgacgct gtcatccacc
     1321 ggctccctga cggccgaagg gcggccggtg acgatcgtga tgctgtatag cccgaccaac
     1381 cagttcatcg gaacggacac gatcatccca cgatcgacca acctctggca gtatggccat
     1441 ccatagcgtg gtacggcggg tcgcgatcct gggcgctctc atcggtgctc tgtcctgcga
     1501 gcacacgcag cccttctcaa acccgggtta cgagtccacg acacccctgc aaccggggaa
     1561 tccagctcag ttgacctaca accccggcgc ggatgtccgg ccgtcgtggc ttccggatgg
     1621 gtcggcgttc ctctacacgc aggaacagac ggatatcaca gagcgcgatc gctgcctcgt
     1681 ggtgatgccc aagacgggcg gcgggatcac ccggacgatt tgtgccgccg atgacttcgc
     1741 gcatgacacg ctgaacgacc tcgagtccgc tgccgtgaat accgcggcca ggatggccta
     1801 tgtccgcacg agcatgctgg cgggcatagg gcgtgcgggc cccgaccacg cagccatggt
     1861 cctgggctcc tacggcactc cggtgcctgg gaccgtcctg cacgtcctgc cgtaccccag
     1921 cccctcaggg caaggagtcg acatggcgtc cgacctgagc tgggtcgggc cgtcggcgct
     1981 ggtctacctt ggagagcaag tgacttaccc cgcggtatgc gcgggatgcg tgacggtcga
     2041 cacggtccgg tcgggagtcg agatcgatcg aatcgacttg aatgggacga tcagcgtcgt
     2101 ggtcgacacg gggttcccca cctcggcgtc cgtcgtcggc ggagacacgc tgtactacac
     2161 attgacgaac agcggaacga ttcaccgccg gatcctcagc acggcggccg atacggtcgt
     2221 gttcgactac ggcctcccgg cgactgacgt ctcggcggtg cggggacggc tcgccgtggt
     2281 tgtgctgggg caattgcacg tgctgtccct ttccccgttc gccgaccaga cgctgacgac
     2341 cccccatccg ggacagatca tcgcccatcc ggctctcgat cctgccgggc acttgatcgt
     2401 cgccgaggtc tctgactcca cggcactctc gaatctctgg ctctggacgg ttccatgaat
     2461 cgaacctggt tggtgttgtc cctcgcgatc gccgctcccc tcgcggccca gtccaccggc
     2521 gccatcgcgg gccgggtgac cgacgattcc accaaacacg cgatcggcgg cgcgttgatc
     2581 tgggtggatg gcgggcgcca aggcgcgacc acggacacgg gtggtgccta tcgggtgcgc
     2641 gacgtccggg ccggctggca ccgggtccgg gcggcgcgga tcgggtaccg ctccgtgatt
     2701 ctggacagcg tgttcgtgcg gagcggcgaa acggcgacgc tcgatttcgc gctcgcgcct
     2761 caggcactca ccatcgattc catcgtagtc cggggtgaag tggatcaggt cctcaaccct
     2821 ctggcgacgg ccgatgtgca ggccatcacc ggcaacgaaa tcaggcggct gccggtcacc
     2881 accgtggccg aggccgtggc tctgagtgcc gggacggtgg gtgagagcta tcgcggaggc
     2941 cgcatcggcg aatcggcctt cgtgctggac gggctcggcg tgaagaatca gctggacgcg
     3001 tccaccaaca ccctcggcat ccaggtgccg acggagatcc tgaccgaggc ctcactcgtc
     3061 accaacgcgt tttccgcccg gtacgggcag gcgttgtcgg gcatcatcaa cgtcgtgacc
     3121 cgggacgcgg gcgaccactg gtcagggcgg gcggcgtacg aaggggaccg gcccatgccc
     3181 caagccgacg atcatgggct cgaccgcgcc gtcttcacgg cggacggccc gctgggccga
     3241 ggcatcgggt tcgtgggggt cctcaatgcg caagggcaga tcgacgggga tccggtcagc
     3301 gcccccgcgc cgaccgaccc gcacgatcct cggactgccg agccctggat ccttccccat
     3361 aacggcgcgg agcgtctgga cgccgcgggg aagctcatca tcccggtcac ccagcagaac
     3421 acgatccggc tcttcggcct gcggtcggcg gcgcagcagt atctgttcga tcccgccttc
     3481 aagtacgacg agactttcgc cccggcgacc cggacggtcg ggaacctgta ctcggcgcac
     3541 tggcagcaca ccgcggcacc gcaggcggtc cacccgttcg gcgccgatgt ccgggtcggg
     3601 tacttcaatc aggatttcat ccgcggacag accaccaaca cgcccggcga tctggtgggg
     3661 gccttcagcg ggcagtccct gcattttctc gatgaaggct tagcccgggc caaggacacc
     3721 gtcgccgcca tgaatgcgct gcccggttac acacccccgg cattgagcgc caattctccc
     3781 tggggcgtgc cggccttctt cctgagcaac ggctcgcggg gtgaactggc ctggaaccgg
     3841 tatcgcgagc tgcggttcca gggggacttc acgctcgccg ccggtccgac cacggacttc
     3901 aatttcggcg gcgaattcga acagcagcac gtcgaaacgt tccagcgggt cgacgcctac
     3961 gtgccggtgg ccgcggtcaa ggattcggtc cctccgccaa ccgcttcgga tttccggcct
     4021 cagagcgcgg cgctgtacgg tgagacgcag atgcagatcg aggacatcgc gctgacgatc
     4081 ggactgcgct acgaccagtt cgacccccgg aacaccactg tgggccagcg cttcggggcg
     4141 cgccgcacca tatcgccgcg gttcgcgatg tcgacggtgc tgcacggggc gaccttcgtc
     4201 gcgagctggg gtcacttcgc gcaggccccc gacttccagt acctggtcaa ttcggcgttc
     4261 gacgacacca cccgcaccgg ccgcttccgg caggggaacc ccgatctcgg gttcgaaacg
     4321 gcgacgcagt acgagttcag cgtgcgggga cggccgagcg agcaaacgtc cctgcgcgtc
     4381 aacgccttcg tgaagcgcct cgacggcctc gtcgcgtcgg tgccgctttc ggtgaatccc
     4441 gattccgcca tcttcggcaa caccgacttc ggcaccgtga agggtgtcga gctgattctc
     4501 gagcgggagc ttcgcgattg gtggggcgcc cggatttcgt acacgttgca ggacgcccag
     4561 gcgacgtcga cgaacccctt cctgctgctc aataacttca cgatcgacca gcagggcgac
     4621 accatccgtt cggcccgcgt ccagttcccg ctcgactacg accggcgaca cgcgctcacg
     4681 gtggtgttgc aggcccgggt gccggacgac ctcggtccgc ggatcttcgg gtcgagggtc
     4741 ttcggggggg tcgaggctgc cgtgatcggc cgctacgact cgggtttgcc gtactccaag
     4801 accaacttgg cgggggactc gatcctcggc ctcccgaaca gtcagcgcct gccgtcccag
     4861 acgcagatcg acatgctgct gcgccgctcc atcagcctgc tcggctggcg gggaggcgtc
     4921 tacctcgatg tgcggaacct gctgaacgcc gcaaacatcg aagccgtccg gcgggatgtg
     4981 ggtagtccgt ttccgtcgag cggcgagctc cagaccatgg cgacgaacgc gtacaacgcg
     5041 aatccgcagc cgatcccata cgaatcgccc cggtaccggg cgtgggcgga cctgaatcac
     5101 gacgggtaca tctcgggaca ggcggagctg atgccgctct acctcgccgc ggcgaaggat
     5161 ttcacccagc ccatcttcca gttcggaccg ccccgcctct tccgactcgg cgtcgagctg
     5221 gagttctagt cccgggcttc gctccggtag ctggtggcca cgccaccgac cacgagcaac
     5281 agaccgagcc agccgagggc agagaggtgc tggctgaaca gtatcagcgc cagaagcgtg
     5341 ccgaccaccg gttcgacggt agcggccacg gcggtggggg cggcgtcgat ccgccgaacg
     5401 ccggcgaaga agaggaagtt ggcgcccacc actggcccca cggcctgcgc gaggcagaac
     5461 gcccacgccg ccaggccttc cggtcgcgcg ctgtaggtga agggtgtacg gtggaagagc
     5521 gtgaggaaga tcgccatcat caccaccccg cctgccagct ccagaaacag cgaccggatg
     5581 ctgccgtagc gcgggaccga ccagcgggcg agcagggtcg tcccggcgta tgagaacatc
     5641 gcgatcaatc cacccaggac ccccaccttg aatccgggcc cccccgacag caccgctccg
     5701 acgtctccgc cgctcaccgt caggcccacc ccgatcatga ccagcaccgc gagaaacatc
     5761 cgcaacgggg tcagcgcctc accg
//
LOCUS       DASUBP010000171         2331 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_58904, whole genome shotgun sequence.
ACCESSION   DASUBP010000171 DASUBP010000000
VERSION     DASUBP010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2331)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2331)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2331
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58904"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..796)
                     /gene="surE"
                     /locus_tag="VFU75_06445"
     CDS             complement(47..796)
                     /gene="surE"
                     /locus_tag="VFU75_06445"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HEU5466510.1"
                     /translation="MDILISNDDGVLAQGLGVLAEACSTLGKVTVVAPDREQSGTSHS
                     LTLHRPLRATRRADGAYQVDGTPTDCVLLALGALMPDKPDFVISGVNHGPNMGEDVLY
                     SGTVAAAFEGLAAGIPSVAVSYGSFDLEHLDSYRSRLQELLADVVAVSDFPKETLLNV
                     NLPAIAAAKVKGVKVTHLGSRVFHEEIARMKDPWGRDIFWIGGGHVTWSGGADSDFQA
                     VRDGYISVTPLHVDMTNYRLLDDVRSWKLGE"
     gene            complement(784..1509)
                     /locus_tag="VFU75_06450"
     CDS             complement(784..1509)
                     /locus_tag="VFU75_06450"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5466511.1"
                     /translation="MPLLADPDGGTSGTALAVVIPAYQAAATIRQVWSGVLDAVPHAA
                     VWVVDDGSTDGTAGQVPGDQVLRHPVNRGKGAALRTGITKALERRPAWIVTLDADGQH
                     PPEEIPRLLEPLERGLADLTLGARARSGAMPLPRRFTNWLSSTLATRIGGVGVPDAQT
                     GFRAFSAHLARSVNPSEAGYDFEAAFLLASLAGGWRVLSVPVPTIYGGSRSHFKAGVD
                     TWRMARVFWKYAGRIVSGARWTS"
     gene            complement(1493..1870)
                     /locus_tag="VFU75_06455"
     CDS             complement(1493..1870)
                     /locus_tag="VFU75_06455"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEU5466512.1"
                     /translation="MSIPHPVQEFFSIGEVCQLTDLKPHVLRYWESQFRFLNPAKNRS
                     GNRVYQRREIELIMLVKHLLYTEKYTIEGARQKIEAYRRSGDLKPEARKALGAETVRD
                     LQGELKAVLAILDGNASPHAPPR"
     gene            complement(1867..>2331)
                     /locus_tag="VFU75_06460"
     CDS             complement(1867..>2331)
                     /locus_tag="VFU75_06460"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEU5466513.1"
                     /translation="ITGVELGGALKNVIAVAAGMVDGLGFGYNTRAALITRGLAEITR
                     LGLALGAEPMTFAGLAGLGDLILTATGALSRNHSLGVELGKGKTLEQALAGKQSVAEG
                     VGTARTAVALGERHHVDLPIARQVAAILFEGKTARQAIADLMEREPKPEQWR"
BASE COUNT          370 a          822 c          751 g          388 t
ORIGIN      
        1 atcgcgtcga cgaggcggac gcggtacccg ccatacgtgt cgccgatcac tcgccgagct
       61 tccaggaccg cacgtcgtcc agaagccggt agttggtcat gtccacgtgg agcggcgtga
      121 cggaaatgta gccgtccctg acggcctgga agtccgagtc agcgcctccg gaccacgtca
      181 cgtgtccgcc cccgatccag aaaatgtcgc ggccccaggg gtccttcatc cgggcgattt
      241 cctcgtggaa gacccggctc cccagatggg tcaccttcac gcccttgacc ttcgcggccg
      301 cgatggccgg caggttcaca ttcagcagcg tctccttcgg gaagtcggat acggcgacca
      361 cgtccgccag aagctcctgc agtctcgagc ggtaggagtc caggtgttcg aggtcgaacg
      421 acccatacga caccgccacc gatggaatgc cggccgccag cccctcgaat gcggcggcca
      481 cggtgcccga gtacaagacg tcctctccca tgttcggacc gtgattcacg ccggagatca
      541 cgaagtccgg cttgtccggc atcagagcgc ccagggcgag gaggacgcag tcggtgggcg
      601 tgccatccac ctgataggcg ccgtcggcgc gccgcgtcgc ccgcagcggc cgatgcagcg
      661 tgagtgagtg actggtgccg ctctgctccc ggtccggcgc cacgaccgtc accttcccga
      721 gcgtgctgca cgcctcggca aggaccccca acccctgggc cagaacgccg tcgtcgttgg
      781 aaatcaggat gtccatcggg ctccgctcac gattctcccc gcgtacttcc agaacacccg
      841 tgccatccgc caggtgtcaa cgcctgcctt gaaatgactt cgggatccgc cgtagatcgt
      901 cggcacgggc accgagagca cccgccagcc gccggccagg ctcgccagca gaaatgccgc
      961 ttcgaagtcg tacccggcct cggaaggatt gaccgaacgc gccaggtggg ccgagaaggc
     1021 cctgaagccg gtctgcgcgt cgggcacccc taccccgccg atccgcgtgg cgagcgtgga
     1081 tgatagccag ttcgtgaacc gtctggggag cggcatggcc ccggaccgcg cgcgggcacc
     1141 cagggtcagg tcggcgaggc cgcgttcgag gggctcgagc aacctgggga tctcttcagg
     1201 cgggtgctgc ccgtccgcat ccagggtcac gatccacgcc ggccggcgct cgagggcttt
     1261 ggtgatcccc gtccggagcg cggcgccctt cccccggttc acgggatggc gcagaacctg
     1321 atcacctggg acctgtccgg ccgtgccgtc ggtggagccg tcgtcgacca cccagacggc
     1381 cgcgtgcggg actgcgtcca ggaccccgct ccagacctga cggatcgtcg ccgccgcctg
     1441 atacgccgga atgaccacgg cgagggccgt cccggacgtg cctccgtccg ggtcagcgag
     1501 gaggggcatg gggcgatgcg ttgccgtcca gaatggcgag caccgctttc agctctcctt
     1561 gaagatccct gaccgtctcc gcgcccaggg ccttgcgggc ctccggcttg agatcccccg
     1621 accggcggta cgcctcgatc ttctggcggg cgccctcgat cgtgtacttc tccgtgtaca
     1681 gcagatgctt cacgagcatg atgagctcga tctcgcgccg ctggtagacc cggttgccgg
     1741 agcggttctt cgcgggattg agaaaccgga actggctctc ccaataccgg agcacgtgag
     1801 gcttgagatc ggtgagctga cacacctcac cgatcgagaa gaactcctgt actgggtgcg
     1861 gtatgctcat cgccattgct ccggcttggg ctcacgttcc atcaagtccg cgatcgcctg
     1921 gcgcgcggtc ttgccttcga agagaatcgc ggctacctgg cgtgcgatgg gcaggtccac
     1981 gtgatgccgt tcgccgagcg cgacggcggt ccgcgcggtt cctacgcctt cggccaccga
     2041 ctgctttccg gcgagtgcct gttccagcgt cttgcccttc cccagctcga cgccgagtga
     2101 gtgattccgc gacaacgccc cggtggcggt gaggatgaga tcaccgagtc cggccagccc
     2161 ggcgaacgtc atcggctcag cgcccagcgc cagtccgagc cgcgtgatct ccgcgagccc
     2221 gcgggtgatg agtgccgcgc gcgtgttgta cccgaatccc agaccatcca ccatgcccgc
     2281 cgcgacggcg atgacgttct tcagggcgcc gcccagctcc acgcccgtga t
//
LOCUS       DASUBP010000172        11524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1190393, whole genome shotgun sequence.
ACCESSION   DASUBP010000172 DASUBP010000000
VERSION     DASUBP010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 11524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11524
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1190393"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2010
                     /locus_tag="VFU75_06465"
     CDS             <1..2010
                     /locus_tag="VFU75_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5466514.1"
                     /translation="HDEDGIFDAVVNGLHDEALGYNFLALFMLDQATQERVMHSSVGW
                     SGDPRLRVAPGAGLSARAITDGKLHYTPDVTRERNYVASLSSGSEVDIPLKVDGVTVG
                     VLVVESTEPNAFDAEDFDILTAAMDQAGIALARVRLLAEERRRADEHAALFDSMAALA
                     GDLELSRVLQVVLDRAVALLNSTGGEVAIFDAQTQELVVSASANIGKDSTGTRLKIGE
                     GAMGHVARTLEPIIIPSYHEWLGQSVQYADWVVHSVMAAPLLIGKRLVGAIAQIHSDP
                     KRVFDKEDLRLLNLFAPQIAVAIENARLYTESQRQKQYFSELVSNSPVAIVTLDTNKT
                     VRSCNPAFEHLFGYTQQEAVGQVLDDLITTEATRREAEAYTREALDRPIAGIGQRRRK
                     DGTLVDVEVLGVPVIVDGAHVGPMALYHDITELLRARQDAEAANRAKSQFLANMSHEL
                     RTPLNAILGYSELVREEAEDRGLHEFTPDLTKIASAGRHLLTLINDVLDLSKIEAGKI
                     EFVATSFPVRRVIDEVSATVEPLVARKNNKFTVRCGDEIGSMHSDETRIRQVLFNLLS
                     NASKFTERGTIGLEVTRTADEMVFAVSDTGIGMTPEQQAKLFEAFVQADASITSKYGG
                     TGLGLAISRRLCRLMGGDVVVDSALGRGTTFTVRLPITAPAAGAD"
     gene            2015..2389
                     /locus_tag="VFU75_06470"
     CDS             2015..2389
                     /locus_tag="VFU75_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5466515.1"
                     /translation="MPRILLVEDNEMNRDMLSRRLIRKGFEVDMAFDGKAGVEKAKAG
                     GYDLILMDMSLPEIDGWEATRQLRASPETKAVPVIALTAHAMAGDREKALEAGCNDYD
                     TKPIELDRLLGKMNALLPAKPA"
     gene            2386..4287
                     /locus_tag="VFU75_06475"
     CDS             2386..4287
                     /locus_tag="VFU75_06475"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU5466516.1"
                     /translation="MSHTAPGSNPVAALRHDLRTPVNHIVGYAEMLIEDLEGPANAGR
                     RAELESTIEAARDALKMISLVLAPTRDTIEPTEVTGLYHQLEDPQHRITEAVHRLLAA
                     AAEAPTATFVEDLKKIEAAAARLVPTTPATGAGAQGSANAPASGKARVLVVDDEAGNR
                     DLLGRRLQREGYAVVSAGGGREALDVVTREAVDVVLLDVMMPDLDGLAVLERLKADPA
                     TRDLPVIMISALDDLGAIARCIEAGALDYLPKPFEPVILRARLAASLAEKRFRDQERD
                     LVRALGVVTAAASAVEAGAYQAGQLGTLAQRPDAVGRLARVMDRMATGVGERESSLRG
                     RLERLRAEIGHARKTQEVVSHDHEDRLAAGDTFAGRYRIEAVLGRGGMGTVYRGRDTE
                     LDETVAIKTLRPELVADDESRERFKDEIRLTRRITHRNVVRTHDFGESGGLWYITMEF
                     VEGLTVRQLLDARGTLGVESTLAIGSQLAESLGVAHATGVIHRDIKPQNLLLDPEGVL
                     KVMDFGVARLARGATARHTQAGLIVGTPAYMSPEQLTGEEVDARSDLYSVGVVLFELL
                     TGRLPLDGSTVMALFAKVLAEDPPRPGTLVAGVPPALDELVAHLLAKRAEDRIESAAV
                     LLARLQALA"
     gene            4347..6605
                     /locus_tag="VFU75_06480"
     CDS             4347..6605
                     /locus_tag="VFU75_06480"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HEU5466517.1"
                     /translation="MRLSLRFIIPLIVVLAALAYAVVPLVDNLTLQWFVRDLDSRAAA
                     VATTMQEPLNGMIDAGNQRAIADLFARVTQDERIYAIGFCPSPAAEPIGTNSLPHRIT
                     CQSLDTLTAKAPAGRLVASSNGPVLVTVRPMPTAKDPAGRLVLVHDMSFIVRRSAETR
                     KYLFLFFVALAAVVALITVVVAQLSWRGWVQGLNALLRGEGLLRPGKPYAPELRPIAR
                     DLRTLIQDLVQQRGTQDDEQIAWTPDALRGILRGELEGEEVIVVSNREPYIHQYVDGQ
                     IAVWRPASGLVTALEPVMRACSGVWVAHGGGSADRAVVDRHDRVAVPPEHPAYRLRRV
                     WLTEEEEAGYYYGLANEGLWPLCHIAHVRPTFRTSDWEKYREVNQRFAGAVAEESTSD
                     NPIVLVQDYHFALLPRALREKLPEATVVSFWHIPWPNPEAFGICPWRDELLTGLLGSD
                     ILGFHTRYHCINFLDTVDRFLEARVDRETFTVTYQGKVTAVKRYPISIDFPPAPELVA
                     RPVDECRLAVRARHHLPPDHAIGIGVDRLDYTKGIEERFRAVERLLELEPHRVGRFSF
                     IQIAAPTRSRIAEYQSYEGRVRAMADQINARFPSAAHPPIILNVAHHEAGEVFEYYRA
                     AEVCVVTSLHDGMNLVAKEFVASRDDERGVLVLSQFAGASRELPEAIIVNPYDIDQCA
                     TALQMALAMPVGEQRVRMRLMRALVRDFNVYRWAGRMLIDASGMRRRGRLIEWSPEPE
                     WRVRDPAGERVR"
     gene            6602..7342
                     /gene="otsB"
                     /locus_tag="VFU75_06485"
     CDS             6602..7342
                     /gene="otsB"
                     /locus_tag="VFU75_06485"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HEU5466518.1"
                     /translation="MTGAGEVPTGGPGWAYFFDLDGTLVDLVDVPAEVQVDGGLRRQL
                     TALHQATGGAMALISGRTLRDLDALFPGVQLPAAGQHGLERRGADGRTVRHQPPPGLE
                     EARRILATGDPRLELEDKGLSLALHYRRAPGLGRYADQLVRTAAASAGPGYAVLQGKD
                     IVELKPAGQDKGVAVREFLDEPPFQGRRPIFLGDDVTDEDGFATVNARGGASIKVGAG
                     DTVARWRLRDVRSVRAWLDRALKAVDAA"
     gene            7339..9126
                     /locus_tag="VFU75_06490"
     CDS             7339..9126
                     /locus_tag="VFU75_06490"
                     /inference="COORDINATES: protein motif:HMM:NF012926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HEU5466519.1"
                     /translation="MIDLALVGNGTVGALVNSGGDVVWACFPRFDGDPMFCSLLRAPG
                     PDPSPGIFAVDLADKVREEQEYAVNTPVLVTRLYDRSGGAIEITDFAPKFHQFGRMFC
                     PMMLIRSVRRIAGSPRVRLRIAPAVEYGARLAAVTWGSNHVRYSGPELAIRVTTDAPI
                     SAVLEQNTFFLEDSITLLVGPDETVQGTPAHVGHRMLEETTAYWREWVRSLTIPFEWQ
                     DAVIRAAITLELNVFEDSGAIIAAMTTSIPEAAGTERNWDYRYCWIRDGAFVVNALNR
                     LGNTRTMERYLAYILNVVAAGTGARLQPVYGISGHPVPDERVVPSLSGFMGYGPVRVG
                     NEAHRQVQHDVYGSAILAATHVFFDHRLVRRGDEALFQRLEALGQQAATLADQPDAGI
                     WELRGTARVHTFSSLMCWAACDRLARIARRLGLADRATYWRSTADRVQTMISERAWNA
                     KLGSFVGTADGTGTALDASLLQLVDVGFLKPDDPRFVGTVAAIERTLKRGEFIFRYNE
                     ADDFGTPENAFLLCTFWYINALAAMGRRDEARALFERVLACRNRHGLLAEHVDPKTGE
                     LWGNFVQTYSMVGIIDSAVRLSTRWDQAL"
     gene            9188..>11524
                     /locus_tag="VFU75_06495"
     CDS             9188..>11524
                     /locus_tag="VFU75_06495"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5466520.1"
                     /translation="MTLRHTLRSLRNRPGFSALVILALGLGIGTTTAIFTVVNAVLLK
                     PLPYRDPSRLALVWSKWSNFDKTWVSTAEYLDYQRQTQLFQDVALWNDNGEVTLTGPE
                     GHAESVTSAQATANLLEVLGVTPRAGQMYTAREDIPNGPAVVMVGYDLWQKRWSGDPT
                     LVGKTIQVDGQPYRVTGILPPDFRLPLEFQSRSSVQLIGPMQIDRSAPSRGGHCCYAI
                     GRLQPGATPALVTTEIRTLAAQWVQQGLYPVDMHFQPFSISLDDEVTGPVRPALMLLT
                     AAVVLLLVLTCANVANLMLTRAADRGREMAVRAALGAGLRDMLRLALGESLVLGISGG
                     VVGLGLAWAGVRLLAARAPTTVPRVAELGIDGRVALFALLLAVGTGMLFALVPFARVR
                     RLDLADSLRAGRGQSAGIDRRRVRTALVVCETAFAVLLLVGAGLTIRTLINLLRINPG
                     FDARNVLTMQVSLPAAKYGTVESANTFFHTLGDEVRKLPGVQAAGFARLLPLATEMGD
                     AGLVIQTKPLPPNVPSRQADWQAVSPGYFEAMKIPLLSGRYFDAHDDYTGEQVIILNR
                     ELVKEYFPGEDPLGQMIRVGFGPTAPLRRVVGVVGDVHHNGLLGNFKRGFYLPQDQWA
                     NAYGNPRRSASLVVKTTGDPRDITGQVVGLLRGLDPDLPATDIRLLSDVLASATQEQR
                     FTMGLMAVFALLALTLAAVGIYGVISYSVSQRTREIGIRLALGSDVRGVRDLVLRQGM
                     RPAYLGVALGLGSAVVLTRFLRAILYEVAPLDWVTFGLV"
BASE COUNT         1838 a         3900 c         3906 g         1880 t
ORIGIN      
        1 cacgacgaag acgggatctt cgacgccgtc gtcaacggcc ttcacgacga agccctcggc
       61 tacaacttcc tcgcgctgtt catgctcgac caagcgacgc aagagcgggt catgcactcc
      121 tcggtcggct ggtcgggtga cccccgcctc cgcgtcgccc cgggcgcggg tctctcggca
      181 cgcgccatta ccgacggcaa gctgcactac acgcccgacg tcacccgcga gcgcaactac
      241 gtcgcgtcgc tctcctccgg ctccgaagtc gacatccccc tgaaggtcga cggcgtgacg
      301 gtgggtgttc tggtcgtgga gagtactgag ccgaacgcgt tcgacgccga ggacttcgac
      361 atcctgacgg cggcgatgga tcaggccggg atcgcgctcg cccgggtacg cctgctggcc
      421 gaggagcgcc ggcgcgccga cgagcacgcg gcgctcttcg attcgatggc cgcgctcgcc
      481 ggcgacctcg agctctcccg cgtcctccag gtggtgctcg accgcgccgt cgcattgctg
      541 aactcgaccg ggggagaggt cgcgatcttc gacgcgcaga cgcaggagct ggtcgtctcc
      601 gccagcgcga acatcggcaa ggactcgacc ggcacgcggc tcaagatcgg cgagggagcg
      661 atggggcacg tggcccgcac gctcgagccg atcatcatcc cgtcctatca cgagtggttg
      721 ggtcaatcgg ttcagtacgc ggactgggtc gtccactcgg tgatggcggc tccgctcctg
      781 atcggcaaac gcctggtcgg cgcgatcgcg caaattcatt cggatcccaa gcgcgtcttc
      841 gacaaggaag atctccgtct cctcaatctg ttcgctccgc agatcgccgt cgccatcgaa
      901 aacgcccggc tgtacaccga atcccagcgc cagaagcagt atttttcgga gctcgtgagc
      961 aacagccccg tcgccatcgt cacgctcgat accaacaaga cggtccggag ctgtaatccg
     1021 gccttcgagc acctgttcgg ctacacgcaa caggaggccg tggggcaggt cctcgacgac
     1081 ctgatcacca cggaagcgac ccggcgggag gccgaggcgt acacgcggga ggcgctggac
     1141 cggccgatcg cggggatcgg acagcgccgc cggaaggatg gaacgctggt cgacgtcgaa
     1201 gtgctgggcg tccccgtgat cgtggatggc gcacatgtcg gtcccatggc gctgtatcac
     1261 gacatcaccg agctcctgcg ggcccggcag gacgccgagg ccgccaatcg ggcgaagagc
     1321 cagttcctgg ccaacatgag ccacgagctt cggaccccgc tcaacgcgat cctggggtat
     1381 tcggagctgg tgcgggaaga ggccgaggac cgcggactgc acgagttcac tcccgatctc
     1441 acgaagatcg cctcggcggg ccgccatctc ctgacgctca tcaatgacgt cctggacctc
     1501 tcgaagatcg aggccggcaa gatcgagttc gtcgcgacct ccttcccggt ccgccgcgtc
     1561 atcgacgagg tctccgcgac cgtcgagccg ctggtcgccc ggaagaacaa caagttcacg
     1621 gtccgctgcg gcgacgagat cggctccatg cattccgatg agaccagaat ccggcaggtg
     1681 ctgttcaatc tcctgtcgaa cgcctcgaag ttcaccgagc ggggcaccat cggtctcgag
     1741 gtcacgcgca ccgcggacga aatggtgttc gccgtgtccg acacggggat cggcatgacg
     1801 cctgaacagc aggccaagct cttcgaagcg ttcgtgcagg ccgatgcgtc gattaccagc
     1861 aagtacgggg gcaccggcct cggactcgcg atttcgcgcc gcctctgccg tctgatgggt
     1921 ggcgacgtcg tcgtcgacag cgcgctgggc cggggtacca cgttcacggt ccgccttccc
     1981 atcaccgctc ccgccgcggg agccgactga ccccatgccc agaatcctcc tggtcgaaga
     2041 caacgagatg aaccgcgaca tgctgtcccg ccgactgatc cggaagggct tcgaagtcga
     2101 catggcgttc gacggaaagg cgggggtcga gaaggccaaa gcggggggct acgacctcat
     2161 cctcatggac atgagtctcc cggagatcga tggatgggag gccacccggc agctccgcgc
     2221 gtcgccggaa acgaaggccg ttccggtcat cgccctgacg gcgcacgcca tggcggggga
     2281 ccgcgaaaaa gccctcgagg caggttgtaa cgactacgac accaaaccca tcgagctcga
     2341 ccgtctcctc ggcaagatga acgccctgct gccggcgaaa ccggcgtgag ccacaccgcc
     2401 cccggctcca accccgtcgc cgcgcttcgc cacgatctcc gcacgcccgt caaccacatc
     2461 gtgggctacg cggaaatgct catcgaggat ctcgaagggc ccgcgaatgc cggccggcgg
     2521 gcggagctcg aatcgacgat cgaagccgcg cgggacgccc tcaagatgat ttcgctggtt
     2581 ctggcgccga cccgcgatac gatcgaaccc accgaagtca ccggtctcta ccaccagctc
     2641 gaggatcccc agcatcgcat caccgaggcg gtgcaccggc tcctggccgc ggccgccgaa
     2701 gccccgaccg cgaccttcgt cgaggacctc aagaagatcg aagccgccgc ggcgcgcctc
     2761 gtgccgacga cgccggcgac cggggcgggc gcccagggtt cggccaacgc cccggccagt
     2821 gggaaagccc gcgtactggt ggtggatgac gaggcaggga accgggatct cctgggccga
     2881 cggctccagc gcgaagggta cgccgtggtg tcagcgggcg gggggcgcga agcgctcgac
     2941 gtggtcaccc gggaagcggt ggacgtcgtg ctcctcgacg tgatgatgcc ggacctcgac
     3001 ggcctggcgg tgctcgagcg cctcaaggcc gaccccgcca cccgtgattt gccggtcatc
     3061 atgatttcgg cgctcgacga cctgggcgcc atcgcccgct gcatcgaagc gggcgctctc
     3121 gactacctcc ccaagccctt cgagcccgtg atcttgcgcg cccggctcgc cgcgtcgctg
     3181 gccgagaagc gcttccggga tcaggagcgg gacctggtgc gtgccctcgg cgtcgtgacc
     3241 gccgcggcga gcgcggtaga ggcgggcgcc taccaggccg gccagctggg gaccctcgcc
     3301 cagcgccccg atgccgtcgg gcgcctcgcg cgcgtgatgg accgcatggc gacgggggtc
     3361 ggcgaacggg agtcgagcct tcgcggacgg ctggagcgac tccgcgccga gatcgggcac
     3421 gcccggaaaa cccaggaagt ggtgagccac gaccacgagg atcgtctggc cgccggggat
     3481 accttcgcgg ggcgctaccg gatcgaggcg gtactcggcc gcggcgggat ggggacggtc
     3541 taccgcgggc gcgacaccga gctggatgaa acggtcgcga tcaagacgct tcgtcctgag
     3601 ctggtggccg acgacgagtc gcgcgagcgc ttcaaggacg aaatccgtct cacccgccgc
     3661 atcacgcacc gaaacgtcgt tcgcacccac gacttcggcg aatcgggcgg gctctggtac
     3721 atcaccatgg agttcgtcga gggcctgacc gttcgtcagc tcctcgatgc ccgcggcacg
     3781 ctcggcgtcg aatccaccct cgccatcgga tcacagctcg ccgagtcgct cggtgtcgcg
     3841 cacgcgaccg gcgtgatcca ccgggacatc aagccgcaga acctcctgct cgatcccgag
     3901 ggcgtgctca aggtgatgga cttcggcgtc gcgcgcctcg cgcgaggcgc cacggcgcgg
     3961 cacacgcagg ccggattgat cgtcggcacg ccggcctaca tgagtcctga acagctcacc
     4021 ggtgaggaag tcgacgcgcg cagcgacctg tattccgttg gcgtcgtgct cttcgagctc
     4081 ctgaccggcc gattgccgct tgacgggagc accgtaatgg ccctttttgc gaaggtgctg
     4141 gcggaagatc ccccccgacc gggcaccctc gtggctggcg tgccaccggc gttggacgag
     4201 ttggtcgcac acttgctcgc caaacgcgcc gaggaccgga tagagtcggc cgcggtactg
     4261 ctcgcccggc tgcaagcgtt ggcatagcag ggccctccga cagtcgaggc gggtaggggg
     4321 actcacgata accccggacc gcggacatgc gtctttccct ccggttcatc ataccgctga
     4381 tcgtcgtcct ggcggcgctg gcctatgcgg tggtaccgct ggtcgacaac ctcacgctgc
     4441 aatggttcgt ccgtgacctg gacagccggg ccgcggcagt ggcgacgacc atgcaggagc
     4501 cgctcaatgg catgatcgac gcgggcaatc aacgcgcgat cgccgacctg ttcgcccggg
     4561 taacgcagga cgagcgcatt tacgcgatcg ggttctgtcc ttccccggcc gcggagccga
     4621 tcgggaccaa ctccctgccg caccgcatca cctgccagtc cctggatacg ctgaccgcca
     4681 aggccccggc ggggcggctg gtggcaagct cgaacggacc ggtgctcgtg acggtgcggc
     4741 cgatgccgac ggccaaggat ccggccgggc gcctcgtgct ggtgcacgac atgagcttca
     4801 tcgtccgccg cagtgccgag acccggaagt acctctttct cttcttcgtg gcgctcgccg
     4861 ctgtcgtcgc gctgatcacc gtggtggtgg cgcaactgtc gtggcgcggc tgggtgcagg
     4921 gcctgaacgc gttgctccgg ggagagggcc tcttgcggcc cgggaaaccc tacgctcccg
     4981 agcttcgccc catcgcgcgc gatctccgaa ccctgattca agacctggtg cagcagcggg
     5041 ggacgcagga tgacgagcag atcgcctgga ccccggacgc cttgcggggc atcctgcgcg
     5101 gcgagctgga gggggaagag gtgatcgtgg tgtccaaccg cgagccctac atccatcaat
     5161 atgtcgacgg ccagatcgcg gtctggcggc ccgccagcgg gctggtcacc gccctcgagc
     5221 cggtgatgcg tgcctgctcc ggagtctggg tggcccacgg gggcgggtcg gccgaccggg
     5281 cggtcgtgga ccgccacgat cgcgtcgcgg tccctcccga acatccggca taccgcttgc
     5341 gacgcgtgtg gctcacggag gaagaggaag ccggctacta ctacgggctg gcgaacgagg
     5401 ggctctggcc gctctgccac atcgcgcacg tgcgtccgac gttccgaacc tcggattggg
     5461 agaaataccg cgaagtgaac cagcgctttg cgggagcggt ggcggaggaa tcgacctctg
     5521 acaaccccat cgtgctggtg caggattatc acttcgccct cttgccccgg gccctccgcg
     5581 agaagctgcc cgaggccacg gtcgtctcgt tctggcatat cccctggccc aatcccgagg
     5641 cgttcgggat ctgcccctgg cgggacgagt tgctcacggg cctcctcgga tccgacatcc
     5701 tcgggttcca cacccggtac cactgcatca acttcctcga caccgtggac cgcttcctcg
     5761 aggcccgggt ggaccgggag acgttcaccg tcacctatca gggcaaggtg accgcggtga
     5821 agcggtatcc catctccatc gacttcccgc cggcgcctga gctggtggcc cgtcccgtgg
     5881 acgagtgccg gctcgccgtg cgtgcgcggc accacctgcc gccggatcat gccatcggca
     5941 tcggggtcga ccggctggat tacaccaagg gcatcgagga gcggttccgc gctgtggagc
     6001 gtctgctcga gctcgagccc catcgcgtgg gtcgattctc gttcattcag atcgcggcac
     6061 cgacgcgcag ccggatcgcg gagtaccagt cgtacgaagg acgcgtccgg gccatggcgg
     6121 atcagatcaa cgctcgcttc ccatccgcgg cgcacccgcc gatcatcctg aacgtggcgc
     6181 accacgaagc cggggaagtc ttcgagtact accgcgccgc cgaggtctgc gtcgtgacga
     6241 gtctgcacga cggcatgaac ctggtcgcga aggaattcgt cgcgtcccgc gacgatgagc
     6301 gcggcgtctt ggtcctctcc cagtttgcgg gcgcctcccg ggagcttccc gaggccatca
     6361 tcgtgaaccc ctacgacatc gaccagtgcg cgaccgcgct gcagatggca ctggcgatgc
     6421 cggtgggtga gcagcgcgtc cggatgcgcc tcatgcgggc tctggtcagg gacttcaatg
     6481 tctaccgctg ggccggacgc atgctgatcg acgcttccgg catgcggcgg cggggccggc
     6541 tcatcgagtg gtcgcccgag cctgagtggc gggtgcgcga cccggcggga gagcgggtgc
     6601 gatgacgggt gccggcgagg ttcccaccgg cggacccggg tgggcttact tcttcgatct
     6661 ggacggtacg ctcgtcgacc tcgtcgacgt gccggcagag gtccaggttg acggagggct
     6721 ccgccggcag ctcacggcgt tgcatcaggc gacggggggc gcgatggcgc tcatcagcgg
     6781 ccggacgtta cgcgacctcg acgcgctctt tccgggcgtg cagctgcccg cggccggtca
     6841 gcatggcctc gagcggcgag gcgccgacgg acgcaccgtc cgccatcagc cgccgccggg
     6901 gctcgaggaa gcacggcgca tccttgccac cggcgatccg cgcctcgagc tcgaagacaa
     6961 gggactctcg cttgcgctgc actaccgccg cgctcccggt ctggggcgct atgcggatca
     7021 actcgtgcgc acggcggccg cgagcgccgg tccggggtat gccgtgctcc aggggaagga
     7081 catcgtagag ctgaagccgg ccggccagga caagggcgtg gcggtccggg agttcctgga
     7141 cgaaccgccc tttcagggac ggcggcccat tttcctgggg gatgacgtga ccgatgaaga
     7201 cgggttcgcg acggtgaacg cgaggggagg ggcgtcgatc aaggtcgggg cgggagacac
     7261 cgtcgcccgc tggcgcctcc gtgacgtgcg ctccgtgcgg gcctggctgg accgcgcgct
     7321 gaaggcggtg gacgcggcat gatcgacctt gcgctggtcg gcaacgggac cgtgggggcc
     7381 ctggtgaact cgggcggaga cgtggtgtgg gcctgtttcc cgcgcttcga cggcgacccc
     7441 atgttctgct ctctgctccg cgctccgggc cccgatccgt cccccgggat cttcgccgtg
     7501 gacctggcgg acaaggtccg ggaggagcag gaatacgcgg tcaacacgcc ggtgctcgtc
     7561 acccgtctgt acgaccgcag cggcggcgcc atcgagatca cggactttgc ccccaagttt
     7621 caccagttcg gccggatgtt ctgcccgatg atgttgatcc ggagcgtcag gcgcatcgcc
     7681 ggaagccccc gcgtcaggct ccggatcgcc cccgccgtgg agtacggcgc ccggctcgcc
     7741 gcggtcacct ggggatcgaa tcacgtccgg tattccggtc cggagctcgc catccgggtc
     7801 accaccgacg ctcccatcag cgcggtactc gagcagaaca cgttcttcct cgaggacagc
     7861 attaccctcc tcgtcggccc cgacgaaacg gtgcagggga cgccggcgca cgtggggcac
     7921 cgcatgctgg aagagaccac ggcctactgg cgggagtggg tgcggtccct caccatcccc
     7981 ttcgagtggc aggatgcggt catccgcgcc gccattacgc tcgagctgaa cgtgttcgaa
     8041 gactcgggcg cgatcatcgc ggcgatgacg acgtccatcc cggaggcggc ggggacggag
     8101 cgcaattggg actaccggta ctgctggatc cgggacgggg cgttcgtcgt gaatgccctg
     8161 aatcggctcg gaaatacccg gaccatggag cggtatctcg cctatatcct gaacgtggtc
     8221 gccgcgggga ccggcgcccg gctccaaccc gtctacggca tttcggggca cccggtgccc
     8281 gacgaacgcg tggtgccctc gctttcgggc ttcatggggt acggtccggt ccgcgtcggc
     8341 aacgaagcgc accggcaggt ccagcacgac gtctacggat cggccatcct cgcggccacg
     8401 cacgtgttct tcgaccaccg gttggtccgc cgcggagacg aggcgctgtt ccagcgtctg
     8461 gaggccctgg gccagcaggc cgccacgctc gcggatcagc ccgacgccgg catctgggag
     8521 ctccggggca cggcacgggt gcacacgttc tccagtctca tgtgctgggc ggcctgcgat
     8581 cgcctggccc gcatcgcccg tcgcctgggg ctggcggacc gggcgaccta ctggcgcagc
     8641 accgccgacc gggtgcagac catgatttcg gagcgggcct ggaacgcgaa gctcgggtcg
     8701 ttcgtgggca ccgcggacgg cacggggacg gcgctcgacg cgagccttct ccaactggtg
     8761 gacgtcgggt ttctcaagcc cgacgacccg cgcttcgtcg ggaccgtcgc cgccatcgag
     8821 cgcacgctga agcggggaga gttcatcttc cggtacaacg aagccgacga cttcggaact
     8881 cccgaaaacg ccttcctgct gtgcacgttc tggtacatca atgccctggc ggcgatgggc
     8941 aggcgagacg aagcgagagc gctgttcgaa cgcgtgctgg cgtgccgcaa ccgtcacgga
     9001 ctcctggccg agcacgtgga tcccaagacg ggagagctgt gggggaactt cgtgcagacc
     9061 tacagcatgg tcgggatcat cgattcggcg gtgcgcctct cgacccggtg ggaccaggcc
     9121 ttataagcag actgcaaccc ctcgggcggt gcgtccgtcc aaccatggac tccttcccag
     9181 gtctcccgtg accctccgcc acacgctccg gtctctccgg aatcgccccg gcttttccgc
     9241 gctcgtgatc ctggccttgg gccttggcat cgggaccacg accgcgattt tcacggtcgt
     9301 caatgcggtg ctgctcaagc ccctgccata tcgcgatccc tcccggctgg ccctggtctg
     9361 gagcaagtgg agcaatttcg acaagacctg ggtgtccacc gccgagtatc tcgactacca
     9421 gcggcaaacc cagctcttcc aggatgtggc cctgtggaac gacaacggtg aggtcacact
     9481 caccgggccc gagggacatg ccgagagcgt gacgagcgcg caggcgacgg cgaacctgct
     9541 cgaggtgctc ggcgtgactc cccgggccgg gcagatgtac accgcccgag aagacatccc
     9601 gaatggtccc gccgtggtga tggtcggcta cgatctctgg caaaaacgat ggagcgggga
     9661 ccccaccttg gtggggaaaa ccattcaggt ggacggccag ccctatcgcg tgacgggaat
     9721 tctcccgccc gatttccgcc ttccgctcga gttccagtca cgttcctccg ttcaactcat
     9781 cggtccgatg caaatcgacc gctcggcgcc atcgcggggc gggcactgct gctacgccat
     9841 cggcagactc cagcccgggg cgacgcccgc cctcgtgacc actgagatca ggaccctggc
     9901 cgcgcagtgg gtccagcagg ggctctatcc cgtagacatg cacttccagc ccttctcgat
     9961 ttctctcgac gacgaggtca ccggcccggt gcgcccggcc ctgatgctcc tcactgcggc
    10021 ggtggtcctg ctgctggtcc tgacctgtgc gaacgtcgcc aacctgatgc tgacccgcgc
    10081 cgcggaccgg gggcgcgaaa tggcggttcg cgcggcgctc ggcgccgggc ttcgggacat
    10141 gctgcgtctc gcgctcggtg agagtctggt gctcgggatc agcggaggcg tggtgggatt
    10201 gggactggcg tgggcggggg ttcgcctgct cgccgcgcgc gcgcccacca cggtgccgcg
    10261 cgtggcggaa ctggggatcg atgggcgggt ggcgctgttc gcactgctgc tcgcggtcgg
    10321 cacgggaatg ctcttcgcgc tcgttccctt tgcgcgggtc aggcggctgg acctcgccga
    10381 ttccttgcgg gcgggccggg ggcagagcgc cgggatcgac cggcggcggg tgcggaccgc
    10441 gctggtggtc tgcgaaacgg cgttcgcggt attgctgctg gtcggtgcgg ggctcaccat
    10501 ccgcacgctc atcaatcttc tgcgcatcaa tcccggattc gacgcgcgga atgtgctcac
    10561 gatgcaggtc tccctgccgg ctgcgaagta cggcaccgtg gagagcgcga atacgttctt
    10621 ccacacgctg ggcgatgaag tccgcaaact ccccggcgtc caggctgccg ggttcgcgag
    10681 actgctcccg ctcgccaccg agatggggga cgcgggactg gttattcaga ccaaaccgtt
    10741 gccgccgaac gtgcccagcc gtcaggctga ctggcaggcg gtcagtcccg gatatttcga
    10801 ggcgatgaag atccccctgc tgagtggccg gtacttcgac gcacacgacg actacaccgg
    10861 cgagcaagtg atcatcctca accgcgaact cgtgaaggag tattttccgg gagaggaccc
    10921 gctgggccag atgatcaggg tcggttttgg gccgactgct cccttgcgtc gggtggtggg
    10981 ggtcgtaggg gatgtccatc acaacggact gcttgggaac ttcaagcggg gcttctactt
    11041 gccacaagac caatgggcaa acgcgtacgg caacccccgc cggtcggctt ccctggtggt
    11101 gaagaccacc ggggacccca gggacatcac cggtcaggtc gtgggtctcc tgcggggtct
    11161 cgacccggac ctgcccgcca ccgatatccg cctgctctcg gacgtgctgg cttcggcgac
    11221 gcaggagcag cgcttcacca tgggactgat ggcggtcttc gccctcctcg cgctcacgtt
    11281 ggcggcggtg gggatttacg gggtgatctc ctacagcgtc agccagcgga cccgcgaaat
    11341 cggcattcgg ctggccctgg gctcggacgt gcggggggtg cgggatctgg tgctgcggca
    11401 ggggatgcgc ccagcctatc tcggggtcgc gctgggactt ggaagcgcgg tggtcctcac
    11461 gcggttcctg cgggccatcc tctatgaggt agcgcccctc gactgggtga ccttcggact
    11521 cgtt
//
LOCUS       DASUBP010000173         5045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1217375, whole genome shotgun sequence.
ACCESSION   DASUBP010000173 DASUBP010000000
VERSION     DASUBP010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5045
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217375"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="VFU75_06500"
     CDS             <1..1227
                     /locus_tag="VFU75_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466521.1"
                     /translation="PGILPGGDWAAGELSSGQLALLSLKDGSELAVTRRGVLPFDSVS
                     QADLLFGESPQYLRSGHLLYGTGDGVLAAMPFDGSARRVLGESAPIVNGMRMEGGYGC
                     AEFSVADDGTLIYVPGRNQLYVNIAFVGANGRFDTLQIPRGPYTQTRISPDGTRLAVQ
                     MRNPVGGWQVMVFDMSSGVRQIVPVAGNYRAFPSSWLSNRDLMIGIWDPVQFLNYGLR
                     LQSLETGKWSDIRLHGASYLTPAPDGKSFVFSDWRSGDLFLRALSEQDTVRTPVPAKG
                     FAASFSPDGRWLVWGAIDGSVAASPVPPTGAVYPVAEHGQFPLWTPKGDAVVYRDGNR
                     YYRSPITTAGGFRAGRAEVLAEGPFMGSFAWTNAISPTGRLLVLLSSPERESGFLGAI
                     TSFPDIVSRMTTTKTR"
     gene            1321..3207
                     /locus_tag="VFU75_06505"
     CDS             1321..3207
                     /locus_tag="VFU75_06505"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU5466522.1"
                     /translation="MRVAALSLLAFGVPVLLPAQTLYTPRAVTHAYKVGARSLDGRPG
                     PNYWQNHARYNIAISLAPPARQVTGTEQITYFNESPDSLRNLVFRLILNIHKPGAPRA
                     FPANEDYLTSGVHVDSFVINGAPRPWGNDAQVFTLRGVRLPSPLAPHDSVRILLNWHY
                     DLSKQSNREGMLDSTTAFLAYFYPRVDVYDDYDGWDGTTFTDMQEFYSDFNDYDVSVK
                     VPANFIVWGTGTLTNPDDVLAPEFAQRLKTSMTSDETVNIATQDDLKAHRVTQADGDH
                     TWRFTATHIPDMTFAVSDHYDWDGGSVVVDDATGRRASVQAAYNDTSVDYKHMVQFGR
                     HALGWLSHNWPGVPYPYEKSTVVEGRADMEYPMMVNDNSFADTVFARFVVEHEMAHTY
                     FPFYMGINESRYAMMDEGWATTFEYLIGQADVGPARATDFYKRFRVNGWAADPSPLED
                     LPIITPNDAENGQAYGNNAYGKPSIGYLALKDMLGDAAFGKALHEFMDRWHGKHPIPW
                     DFFYTFNNVTGKNLNWFWNSWYFSNTYIDLAPAGVTGSTVKVKNIGGMPAPFDLLVTY
                     SDGSQDSLHETSAVWEKTPTQASIGLKSRKTVRSVTLKHGVWVDADTTNDTWARASAP
                     TPRK"
     gene            complement(3176..4102)
                     /locus_tag="VFU75_06510"
     CDS             complement(3176..4102)
                     /locus_tag="VFU75_06510"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional helix-turn-helix transcriptional
                     regulator/GNAT family N-acetyltransferase"
                     /protein_id="HEU5466523.1"
                     /translation="MAVRNEQIAAVRRFNRFYTRQIGLLDEHLNGSPFSYPEARIMYE
                     LAHNKDASASDLVVGLGMDAGFVSRILGRLERRGLITRRRSEADGRVSQLNLTARGQV
                     QFAELDAASQRDMSRMLGRVKGDGPDRLIRAMRTIQDLLGFRGTVMRPPIRLRRPRPG
                     DLGWVVERNAAVYAKEYGWGLGAEALFARIVADYQQHHDPERERCWIAERWGDRVGCV
                     FLVKRTGTVAQLRMLLVEPGARGSGLGRRLVDECTQFAREAGYRKIVLWTNRVLSAAR
                     HIYESAGYRFVKEERHDELDTDPIFEVWELKL"
     gene            4297..4734
                     /locus_tag="VFU75_06515"
     CDS             4297..4734
                     /locus_tag="VFU75_06515"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HEU5466524.1"
                     /translation="MAKLVLMAEATGKQPIIVTHLGGMQFCAEVRGHRIVVDQPTKVG
                     GTDSAPTPIELLTSSLGCCVAFYVQQFCRSRSLPYQGMSVEVTPQTTAGRIDRFTVRV
                     VMPEPLPTRETELLERVARSCPAHGTLAHGADIAIGIEVPVTT"
     gene            complement(4764..>5045)
                     /locus_tag="VFU75_06520"
     CDS             complement(4764..>5045)
                     /locus_tag="VFU75_06520"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HEU5466525.1"
                     /translation="VTKRRVSAFAGSDLDVVLRAGNTDTLILCGIATSGVVLSTVRQA
                     ADLDYRLIVLSDGCLDMDPEVHRVLTEKVFVRQADVKTIAEWMQEVGAG"
BASE COUNT          889 a         1660 c         1578 g          918 t
ORIGIN      
        1 cctggcatcc tcccgggcgg cgactgggcc gcgggcgaac tgagttcggg ccagctggcc
       61 ctgctctcct tgaaggacgg ttcggagctc gccgtcaccc gccgcggcgt gttgccgttc
      121 gattccgtga gtcaggcgga cctcctgttc ggggagagtc cgcagtacct ccggtccggt
      181 cacctgttgt acggaacggg cgacggcgtg ctcgcggcga tgcctttcga cggctcggcg
      241 cgacgcgtcc tgggggaatc ggcgcccatt gtgaacggga tgcggatgga gggcgggtac
      301 gggtgcgccg aattctcggt cgccgatgac ggcaccctga tctacgtccc gggccggaac
      361 cagctctacg tcaacatcgc attcgtcggt gccaacgggc gattcgacac actccagata
      421 ccccgcgggc cgtacaccca aacccgcatc tctcccgacg gcacgcgtct ggccgtgcag
      481 atgcggaacc cggtcggtgg ctggcaggtc atggtcttcg acatgtccag cggcgtgcgg
      541 cagatcgtgc ccgtcgcggg caactatcgc gcgtttccgt catcctggct ttccaaccgg
      601 gatctcatga tcggcatctg ggatcccgtg cagttcctga actacggcct tcgtctccag
      661 tccctcgaga cggggaaatg gtctgacatt cgcctgcacg gcgcgtcgta tctgacgccg
      721 gcacccgacg gaaagtcgtt cgtgttctcc gactggcgct cgggcgacct gttcctgcgg
      781 gcattgagcg agcaggatac cgtgcgcacg ccggtccccg ccaaaggctt tgcggcgtcg
      841 ttctctcccg atggccgctg gctggtctgg ggcgcgatcg acggatcggt ggcggcttcg
      901 ccggtgcccc ccacgggcgc cgtctaccct gtggctgagc acgggcagtt tccgctgtgg
      961 accccgaagg gggacgcggt cgtgtaccgg gatggcaacc ggtactaccg gtcccccatc
     1021 acgacggcgg gcgggttccg ggccgggcgg gccgaggtgc ttgccgaggg cccgtttatg
     1081 ggatcgttcg cctggaccaa tgcgatcagc cccacgggcc gattgctcgt gctgctgtcg
     1141 agtcctgagc gggagagtgg atttctggga gcgatcacat cgtttcctga catcgtgagc
     1201 cgcatgacca ccacgaagac ccgctgaggc gcgcccagtc gacgcaaccg tccggccaat
     1261 cggtgaatct cagaggttgg atccgcgtag ttacctgacc atacccagga gttgttctcg
     1321 atgcgcgtag ctgctttgtc cctcctcgcc ttcggtgtgc ctgttctctt gcccgcccaa
     1381 acgctctaca cgccccgcgc ggtcactcac gcgtacaagg tcggcgcgcg gtcgctcgac
     1441 ggccggcccg ggccgaacta ctggcagaat cacgcgcggt acaacatcgc gatcagcctg
     1501 gcgcccccgg cgcggcaggt gaccggcacc gagcagatca cgtatttcaa cgaaagtccg
     1561 gattcgctgc ggaacttggt gttccggctc atcctcaaca tccacaagcc cggggcgccg
     1621 cgcgcctttc ccgccaatga ggactacctg acgtcggggg tccacgtcga ttcgttcgtg
     1681 atcaatggcg cgccgcggcc ctgggggaat gacgcacagg tcttcaccct gcgtggcgtt
     1741 cgcctgccgt ctcccctcgc ccctcacgat tccgtccgaa tcctgctgaa ttggcactat
     1801 gatctgtcga agcagagcaa tcgcgagggg atgctcgatt cgacgaccgc cttcctcgcc
     1861 tacttctacc ctcgggtcga cgtctacgac gactacgacg gctgggacgg caccacgttc
     1921 accgacatgc aggagttcta ttcggacttc aacgattacg acgtgtctgt gaaagtcccg
     1981 gcgaacttca tcgtctgggg cacggggacc ctgaccaatc cggatgacgt gctggccccc
     2041 gagttcgcgc agcggttgaa gacgtcaatg acgtcggatg agacggtgaa catcgcgacc
     2101 caggatgact tgaaggccca ccgtgtcacg caggccgacg gcgatcacac ctggcgcttc
     2161 acggccaccc acatccccga catgaccttc gccgtgagcg accactacga ctgggatggc
     2221 gggtcggtgg tagtcgacga cgccacgggc cggcgggcca gcgttcaggc cgcgtacaac
     2281 gacacctcgg tcgactacaa acacatggtc cagttcggcc gccatgcctt ggggtggctg
     2341 tcccacaact ggcccggcgt gccctatccc tacgaaaaga gcacggtagt cgagggacgg
     2401 gcggacatgg agtacccgat gatggtgaac gacaattcgt tcgccgacac cgtcttcgcg
     2461 cgcttcgtcg tcgagcacga aatggctcac acgtacttcc cgttttatat ggggatcaac
     2521 gagagccgct acgccatgat ggacgagggc tgggcgacga cgttcgagta tctgattggg
     2581 caggcggatg tgggccctgc ccgcgccacg gacttctaca agcgattccg ggtcaatggc
     2641 tgggccgccg atccgtcgcc actggaagac ctcccgatca tcacgccgaa tgacgccgag
     2701 aatgggcagg catacgggaa taacgcgtat gggaagccgt caatcggcta tctggccctg
     2761 aaggacatgc ttggggacgc ggcgttcggg aaggccctgc acgagttcat ggaccgctgg
     2821 cacgggaagc acccgatccc gtgggacttc ttctacactt tcaacaacgt caccgggaag
     2881 aacctcaact ggttctggaa ctcgtggtac ttctctaaca cctatatcga cctggcaccg
     2941 gctggcgtaa cgggctcgac ggtgaaggtg aagaacattg gcggcatgcc ggcgcccttc
     3001 gatcttctgg tgacatacag cgacgggtca caggactcgc tgcacgagac gtccgccgtg
     3061 tgggagaaga cgcccacgca ggcgtcgatc gggctcaagt ccaggaagac ggtccggtcg
     3121 gtgacgctga agcacggggt ctgggtggac gccgatacga ccaacgatac gtgggctaga
     3181 gcttcagctc ccacacctcg aaaatagggt cggtatcgag ctcgtcgtgg cgttcctctt
     3241 tgacgaaacg gtatccggcc gattcataga tgtggcgcgc ggctgaaagg acgcggttgg
     3301 tccacaatac gattttgcgg tacccagcct cgcgtgcgaa ttgcgtgcat tcgtcgacca
     3361 gccggcgtcc gagccccgaa cctcgggcgc cgggttcgac gagcagcatc cggagctggg
     3421 cgacggtgcc tgttctcttt acgagaaaca cacagcccac gcggtctccc cagcgctcgg
     3481 cgatccagca tcgctcacgc tccggatcgt ggtgctgctg atagtccgcg acgatccggg
     3541 cgaagagagc ctcggcgccc agtccccacc cgtattcttt tgcatagaca gcggcattgc
     3601 gctccaccac ccaacccagg tctccaggcc gggggcgccg aagtctgatc ggcgggcgca
     3661 tgaccgtgcc gcggaagccc agcagatcct gtatcgtccg catcgcccgg atgaggcgat
     3721 ctgggccgtc tcccttcacc cgcccgagca tccggctcat atcgcgttgg gatgccgcat
     3781 ccagctccgc gaactggacc tgaccacggg cggtgaggtt caactgggag actcggccgt
     3841 ccgcctccga tcgccgccgg gtgatcaacc cccgccgctc gaggcggccg aggatccgac
     3901 tgacgaaccc cgcgtccatc cccaggccca ccaccaaatc ggacgcggag gcgtccttgt
     3961 tgtgcgcgag ctcgtacatg atccgggctt cgggatacga gaacggggag ccgttcagat
     4021 gctcgtcgag gaggccgatc tggcgcgtgt aaaaccgatt gaaccggcgg acggcggcga
     4081 tttgctcgtt ccgaacagcc atgggggcat cccgtggggt ccctccgcga tttacggcaa
     4141 ttagttgact gagtcaagta tctggccaac caggaaaaga cggtatcccg accgccgccg
     4201 gacgagtcta cctcggggtg aagggtgaag tcgacgtatt cggcctccga ccctgaagga
     4261 acgttcacga ctctttcacg ttgcgtcctg ggaggcgtgg ccaaacttgt cctcatggcc
     4321 gaagcaaccg ggaagcagcc gatcatcgtg acccatctgg gaggcatgca gttctgtgcc
     4381 gaggtcaggg gtcaccggat cgtcgtggac cagccgacaa aagttggggg aactgattcc
     4441 gcccccacgc cgatcgagtt gctgacgtca tcgctcgggt gttgtgtggc gttctacgtc
     4501 cagcagttct gccgctcgcg gtccctcccg tatcagggca tgtcggtgga agtcacgccc
     4561 cagaccacgg ccgggcgcat cgaccgcttc accgtccggg tagtcatgcc cgagccactc
     4621 ccgacgcgag agacggaact cctcgaacgg gtcgcccgga gttgtcccgc acacgggacg
     4681 ctggcccacg gcgcggacat cgccatcggc atcgaggtgc ccgtgacgac ttgagcttcc
     4741 ggccctgatg ctcgcccagc gggtcaacct gccccaacct cctgcatcca ctccgcaatc
     4801 gtctttacgt ccgcctgcct gacgaacacc ttctcggtca ggacccggtg cacctccgga
     4861 tccatgtcga gacaaccgtc cgaaagcacg atcagccggt agtccagatc agcggcctgc
     4921 cgcaccgtcg aaagcacgac gccgctcgtc gcgatgccgc acagaatcag cgtgtcggtg
     4981 ttccccgccc gtagcaccac gtccagatcg cttcccgcaa acgcactgac ccggcgcttg
     5041 gtgac
//
LOCUS       DASUBP010000174         5290 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_318454, whole genome shotgun sequence.
ACCESSION   DASUBP010000174 DASUBP010000000
VERSION     DASUBP010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5290)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_318454"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VFU75_06525"
     CDS             <1..454
                     /locus_tag="VFU75_06525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5466526.1"
                     /translation="FKGNDLQIRVNPGAGDTASDEVGRLTFMDNAVDTSTGTVLLKGE
                     FANEDRALWPGQFVNVRLELYVEKSATVVPAAAVTTGQTGTFVFVVNPDQTVTLRPVT
                     VERTAGGLAVISQGIKAGETVVTDGQVRLASGSKVSVKTGGSAQMGDQ"
     gene            451..3540
                     /locus_tag="VFU75_06530"
     CDS             451..3540
                     /locus_tag="VFU75_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5466527.1"
                     /translation="MNIAGAFIKRPVMTTLVMFGILLFGIMGYRLLPVNDLPNVDYPT
                     VSVSASLPGASPETMASAVATPLEKQFTTIAGVDQMTSTSTLGSTNITLQFSLDRNID
                     AAAQDVQAMISKTLRQLPQGIIPPSYQKVNPADSPILYMALSTKTLPLSTLDEYAETF
                     LSQHISTVEGVAQVQVFGSSKYAVRIQLDPRQLASRSIGIDQVASAIGAGNVNMPTGT
                     LWGDDKAYTVQANGQISSAPAFRQLIVTYRNGNPVRLGDLGLVLDDMQDNKSFSWFDG
                     TRAIVLAIQRQPGTNTVEVSEAVQKMVAQLSDQIPASVSVNVLYDRAISIRDSVNDVK
                     FTLLLTLLLVVMVIFLFLRNLSATVIPSLALPFSIIGTFAVMYLLGYSLDNLSLMALT
                     LSVGFVVDDAIVMLENIVRHIEMGTPVMQAALEGSREIGFTILSMTLSLTAVFIPVLF
                     MGGIIGRLFHEFAVTIGVAILVSGFVSLTLTPMLCSRFLKAHGGEQHGKFYNASERVY
                     QRVLEAYRRSLEWVMTRRRLALVVSGLILFATVGLFKLVPKGFIPDEDSGSIRGTTET
                     AEGTSYQSMAAHQEQIAAIIAQDPNVAHFMSRVNGGSNQGGVFLHLKPRNERALDAAG
                     VIRELGPKLATIPGITVYLTNPPPINVGGRQSKSLYQFTLQSSDVTELYQGATELTQR
                     LQSQPLLQDVTSDLQIKNPQVSVQIDRDRAATLGVTAQQIETALYDAYGSGQVSTIYT
                     PNNEYWVIMELLPQYQKDLSALNMLYVRSASGSLVPLGAVAKLTASLGPLSVNHAGQV
                     PAVTISFNLRPNVSIGNAVAAVQKEANQVLPSTISTNFAGTAQAFQASQQGLLALLVV
                     AILVIYMVLGILYESFIHPLTILSGLPFAGFGALLTLLIFGTDLSVYSFVGIIMLIGL
                     VKKNAIMMIDFALEAERKEGKGAEAAIVEACLVRFRPIMMTTMAALMGTLPIAIGFGA
                     GSESRRPLGLAVVGGLAFSQFITLYITPVIYTYLDEFQHWLGKARAPLAAPVPHPGD"
     gene            complement(3537..4574)
                     /locus_tag="VFU75_06535"
     CDS             complement(3537..4574)
                     /locus_tag="VFU75_06535"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU5466528.1"
                     /translation="MRFSYGRVPQRVYWEVTRACDLACRHCRADATPSPHPLELDTGE
                     ALRLLEQLAAADPSPHVVLTGGDPLKRADLFTLIAAGRDLGLHISVSPSATPLMTPAV
                     IGQLKEAGVEAISLSLDGSTAEKHDAFRGVPGTFERTLEAGRVAAAVDLPVQVNTLVA
                     EETVDDLAALYRLSAELGAARWSLFFLVGVGRGTVLSPVSPDACEGIMRWAAGLPAGR
                     PVVTTTEAPHYRRVVAERGRRAGAGPGHAAGIRDGNGIMFITHTGDVTPAGFLPIAVD
                     NVRRRHPLEIYRETALFRLLRDPDGFGGRCGRCDYRNECGGSRARAYAATGDPLAEDP
                     LCRYQPAESFV"
     gene            4648..5133
                     /locus_tag="VFU75_06540"
     CDS             4648..5133
                     /locus_tag="VFU75_06540"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5466529.1"
                     /translation="MARTDRRIALLLEVLDQAFGRAAWHGPNLAGSLRGLTWREALKR
                     PRAGRHCIWELVLHTAYWKCIARKRLTRDPELEFPRAPANFPVVPRKPTAALWRQDVV
                     LLRKEHRLLRGVIARFPAARLGRRAWHSHWTNVETIYGIASHDLYHAGQIQLIKALIH
                     P"
     gene            complement(5111..>5290)
                     /locus_tag="VFU75_06545"
     CDS             complement(5111..>5290)
                     /locus_tag="VFU75_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466530.1"
                     /translation="RLGAAAAAALFEEDAAGKKAREHLAEQLRIAPSLSYEGKGRPTK
                     RDRRELDRLKGESEP"
BASE COUNT          883 a         1825 c         1667 g          915 t
ORIGIN      
        1 cttcaagggc aatgacctgc agatccgcgt gaatcccggt gccggcgaca cggcctccga
       61 tgaggtcggg cgcctgacgt tcatggacaa cgccgtcgac accagtacgg gtaccgtgct
      121 gctgaagggt gaattcgcca acgaggatcg cgcgctctgg cccggccagt tcgtcaacgt
      181 ccgcctcgag ctttacgtcg agaagagcgc gaccgtcgtc ccggccgccg cggtcaccac
      241 cgggcagacc gggaccttcg tcttcgtcgt caatccggac cagacggtga cgctgcgtcc
      301 ggtgacggtg gagcggacgg cggggggcct cgcggtcatc tcccagggta tcaaggccgg
      361 cgagacggtg gtgacggacg gacaggtacg gctcgcttcg gggtccaagg tctccgtgaa
      421 gaccggcggg tcggcgcaga tgggggatca gtgaacatcg cgggggcgtt catcaagcgg
      481 ccggtcatga ccacgctggt catgttcggc attctgctct tcggcatcat gggctaccgc
      541 ctcctgccgg tgaacgacct gccgaacgtc gactacccga cggtgtccgt cagcgcgtcg
      601 ctcccgggag cgagtccgga gaccatggcg tcggccgtcg ccacccccct cgagaagcag
      661 ttcaccacga tcgccggcgt ggaccagatg acgtcgacga gcacgctggg gtcgaccaac
      721 atcaccctgc agttctcgct cgaccgcaac atcgacgcgg ccgcacaaga cgtgcaggcg
      781 atgatttcca agaccctgcg gcagctccct caggggatca tcccgccgtc gtaccagaag
      841 gtgaatcccg ccgactcgcc cattctctac atggcgctgt cgaccaagac actgcccctc
      901 tcgaccctcg acgaatacgc cgagacgttt ctgtcgcagc acatctcgac ggtcgagggt
      961 gtggcgcagg tgcaggtctt cggctcctcg aagtatgcgg tgcggattca gctcgacccg
     1021 cgccaactcg cctcccgcag catcggcatc gatcaggtgg cgagtgccat cggtgccggc
     1081 aacgtcaaca tgccgaccgg tacgttgtgg ggggacgaca aggcctacac ggtgcaagcc
     1141 aacggccaga tctcgagtgc ccccgcgttc cgccagttga tcgtgaccta ccggaacggc
     1201 aaccccgtcc ggctgggcga cctggggttg gtcctcgacg atatgcagga caacaagtcc
     1261 ttcagctggt tcgatggcac tcgtgccatc gtgctcgcca ttcagcgcca gcccggcacc
     1321 aataccgtcg aagtgtccga ggcggtgcag aaaatggtgg cgcagttgtc ggatcagatc
     1381 ccggcctcgg tcagcgtcaa cgtcctctac gaccgcgcca tctcgattcg cgactccgtc
     1441 aacgacgtca agttcaccct gctcctgacg ctgctcctgg tcgtgatggt gattttcctg
     1501 ttcctgcgga acctgtcggc gacggtgatc cccagcctgg ccctgccctt ctcgatcatc
     1561 ggcaccttcg cggtgatgta cctcctgggc tacagcctcg acaacctctc tctgatggcc
     1621 ctgacgctgt cagtgggatt cgtggtggac gatgccatcg tgatgctgga gaacatcgtc
     1681 cggcacatcg agatgggaac gccggtgatg caggccgcgc tcgagggctc cagggaaatt
     1741 ggcttcacca tcctgtcgat gaccctgtcg ttgaccgcgg tgttcatccc ggtgctgttc
     1801 atgggcggga tcatcgggcg gttgttccat gagttcgccg tgactatcgg cgtcgccatc
     1861 ctggtctcgg gattcgtgtc gctcaccctg acgcccatgc tctgcagccg gttcctgaag
     1921 gcccatgggg gggagcagca cgggaagttc tacaacgcgt cggagcgggt gtaccagcgg
     1981 gtgctcgagg cctaccgccg gtcgctcgag tgggtcatga cccggcgccg gctggctctg
     2041 gtggtctccg ggctgattct cttcgccacc gtcggcctct tcaagctcgt gccgaagggc
     2101 ttcattcccg acgaagacag cgggtcgatc cggggcacta cggagaccgc cgaaggcacg
     2161 tcctatcagt cgatggccgc gcaccaagag cagatcgcgg ccatcatcgc ccaggatccc
     2221 aacgtcgcgc acttcatgtc gcgggtgaac gggggctcga accagggggg cgtcttcctc
     2281 cacctgaagc cccggaacga gcgtgccctc gatgccgccg gcgtcatccg ggagctcggg
     2341 ccgaagctcg ccactatccc cggcattacc gtctacctca cgaacccccc gcccatcaat
     2401 gtcggcggcc ggcagtcgaa gagcctgtat caattcaccc tgcagagctc ggatgtcacc
     2461 gaactctacc agggtgcgac ggaactgacc cagcgcctcc agtcacagcc cttgctgcag
     2521 gacgtcacct cagatttgca gatcaagaat ccccaggtct cggtgcagat cgatcgtgac
     2581 cgcgccgcga ccttgggtgt caccgcgcag cagatcgaga ccgcgctgta cgacgcgtac
     2641 ggatcgggac aggtctcgac catctacacg ccgaacaacg agtactgggt gatcatggag
     2701 ctgctgcccc agtaccagaa ggatctctcg gccctgaaca tgctgtacgt ccgttcggca
     2761 tcgggatccc tcgtgccctt gggcgcggtc gcgaagctca ccgccagcct cggtcccctg
     2821 tcggtgaacc atgcgggcca ggtgcccgcc gtgacgatct ccttcaacct ccgccccaac
     2881 gtctccatcg gaaacgcggt ggcggccgtg cagaaggaag ccaaccaggt cctgccatcc
     2941 acgatttcca cgaacttcgc cggcaccgcg caggcgttcc aggcgagtca gcagggattg
     3001 ctggcgctcc tggtggtcgc catcctcgtc atctacatgg tgctggggat tctgtacgag
     3061 agcttcattc acccgctgac catcttgtcg ggtctgccct tcgcaggctt cggtgcgctc
     3121 ctcaccctgc tgattttcgg gaccgacctc tcggtctact cgttcgtcgg gatcatcatg
     3181 ctgatcggcc tcgtaaaaaa gaacgcgatc atgatgatcg acttcgcgct cgaagccgag
     3241 cggaaggaag ggaagggggc ggaagcggcg atcgtcgagg cgtgtctggt gcggttccgg
     3301 cccatcatga tgaccaccat ggcggcgctg atgggcacgc ttccgatcgc gatcggcttc
     3361 ggcgccggct cggaatcccg tcgccccctc ggcctggcgg tggtgggcgg cctcgcgttc
     3421 tcccagttca tcacgctcta catcacgccg gtgatctaca cgtacctcga cgaattccag
     3481 cactggctgg ggaaggcgcg cgccccgctc gccgctccgg tcccccaccc cggggactag
     3541 acgaagctct cggcgggctg ataccggcag agcggatctt cggcgagcgg atctcccgtc
     3601 gcggcatagg cccgcgcgcg tgaccctccg cactcattcc ggtagtcgca ccggccgcac
     3661 cgccctccga atccgtcggg gtcccgtaag aggcgaaaga gggccgtctc ccggtagatc
     3721 tcgagcggat gccgccgccg gacgttgtcg accgcgatgg gcaggaagcc cgccggcgtg
     3781 acgtcgcccg tgtgggtgat gaacatgatc ccgttgccgt cccggatgcc cgccgcatgc
     3841 cccggacccg ctcccgcgcg acgcccccgc tcggccacca cccggcggta gtgcggcgct
     3901 tcggtggtgg tgaccacggg gcgccccgcc ggcagccccg ccgcccaccg catgatgccc
     3961 tcgcaggcat cgggagatac cgggctgagt accgtgcccc gaccgacccc gaccagaaag
     4021 aacagactcc atcgcgccgc gcccaactcg gcgctcaacc ggtacagcgc tgcgaggtcg
     4081 tcgaccgtct cctccgccac cagggtgttc acctgcaccg gaagatccac cgccgcagcc
     4141 acccggccgg cctccagtgt ccgctcgaac gtgccgggga caccccggaa cgcgtcgtgt
     4201 ttctcggccg tcgaaccatc cagcgacagc gaaatagcct cgacccccgc ctccttcaat
     4261 tggccgatca ctgccggggt catgagcggc gtggcactcg gggagacgga gatgtgcaac
     4321 cccaagtcgc gccccgccgc gatcagcgtg aaaagatccg cgcgcttgag cggatccccg
     4381 cccgtcagca cgacgtgcgg tgagggatcg gccgcggcca gctgctcgag gagcctgagc
     4441 gcctcaccgg tgtcgagctc gagggggtgc ggggagggcg tcgcatccgc ccggcaatga
     4501 cggcaggcca gatcgcaggc ccgggtgacc tcccagtaga ctcgctgggg tactcgaccg
     4561 taggagaatc gcatgggtgg gacgctcgcc ggaccatcgt gaagaacagc ccaagacagt
     4621 gtgaagggac cagagtatcc ttgagctatg gcacgtactg accgccggat cgcgctcctg
     4681 ctcgaggtgc tggaccaggc cttcggccgc gccgcgtggc acggcccgaa cctggccggc
     4741 agcctgcgag gcctgacctg gcgggaggct ctcaagcgcc cgcgcgcggg gcggcactgc
     4801 atctgggaac tggtcctgca cacggcttac tggaaatgca tcgcgaggaa gcgtctgacg
     4861 cgcgaccccg agctcgagtt cccccgcgcg ccggccaact tcccggtggt gcccaggaaa
     4921 ccgaccgccg cactctggcg gcaggatgtg gtcttgctcc ggaaggaaca tcggttactg
     4981 cggggggtca tcgcccgttt tcctgcggcc cgcctgggac ggcgggcctg gcattcccac
     5041 tggaccaacg tcgaaacgat ttacgggatc gcgtcccacg acctctacca cgcggggcag
     5101 attcagctca tcaaggctct gattcaccct tgagccggtc gagctcccgc cggtcccgct
     5161 tggtcggcct tcccttgcct tcgtagctca acgacggcgc gatccgcagc tgctcggcga
     5221 ggtgttcgcg cgccttcttc cccgccgcgt cctcttcaaa aagcgccgcg gccgccgcgg
     5281 cgcccagacg
//
LOCUS       DASUBP010000175         3071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_18164, whole genome shotgun sequence.
ACCESSION   DASUBP010000175 DASUBP010000000
VERSION     DASUBP010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3071
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18164"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..201)
                     /locus_tag="VFU75_06550"
     CDS             complement(<1..201)
                     /locus_tag="VFU75_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl diphosphate synthase family
                     protein"
                     /protein_id="HEU5466531.1"
                     /translation="MVTADLLAQVRLNGGVPRHVAIIMDGNGRWARGRLMPRPLGHRA
                     GMKAVREVVEGAIESGVEVLTLF"
     gene            complement(201..755)
                     /gene="frr"
                     /locus_tag="VFU75_06555"
     CDS             complement(201..755)
                     /gene="frr"
                     /locus_tag="VFU75_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEU5466532.1"
                     /translation="MSTIPELLKTAKAAMEKSVDSVKREFSSVRTGRATTTLLDVVRV
                     DAYGNMMPLNQVASVAAPEPKLLTVTPWDKSLAKAIEKAIVVSDLGLTPSSDGTVIRI
                     PIPALTEERRKELVKVVHKFAEEGRVGVRHARTDAIAKAKKLEHVSEDEKKHCEKDVQ
                     KLHDEKMKAIDDAVKAKEAEIMEV"
     gene            complement(752..1471)
                     /gene="pyrH"
                     /locus_tag="VFU75_06560"
     CDS             complement(752..1471)
                     /gene="pyrH"
                     /locus_tag="VFU75_06560"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEU5466533.1"
                     /translation="MPALTYKRALLKLSGEHFAGGKGTGAGIDFATLEGITDELKAVA
                     ATGVQLGLVVGGGNILRGTTASQQGMDRVSADYMGMLATVINALALQELLEKKGVDTR
                     VMTAIRMEELAEPYIRRRAIRHLDKGRVVIFAGGTGNPYFSTDTAAVLRALEIEAQVL
                     MKATNVDGVYTADPKKDKTAMFIPELSFQDALVKGYAVMDANAFGLCKENRLPIVVFN
                     INQSGATARILAGERVGTIVR"
     gene            complement(1475..2074)
                     /gene="tsf"
                     /locus_tag="VFU75_06565"
     CDS             complement(1475..2074)
                     /gene="tsf"
                     /locus_tag="VFU75_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HEU5466534.1"
                     /translation="MPEISAKMVAELRNRTGAGMMDCKKALEETGGDLEKATEILRVK
                     YAAKAEKRAEKTAAEGLIDAYIHFTGKVGVMVELNCETDFVARTDEFKQLARDIALQI
                     ASTRPLAVRVEDIPADVVERERRIYEAQVIEQKKPEQIRAKIVEGMLKKFFQESVLME
                     QPFVKDDKVTIGDLVKAYSGKTGEKVHIHRFARFELGGN"
     gene            complement(2159..>3071)
                     /gene="rpsB"
                     /locus_tag="VFU75_06570"
     CDS             complement(2159..>3071)
                     /gene="rpsB"
                     /locus_tag="VFU75_06570"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEU5466535.1"
                     /translation="RRSKTQGKDCMAQPQLQDLLEAGVHFGHQTRRWNPKMRRFIFAE
                     RSGIYIIDLQKTLHQLLQAQELLRSVVLKGEGVLFVCTKKQLKAILQAEAEKCGSYFV
                     SERWLGGTLTNFQTIKKQIKRLKDLEQGASDGEFENYTKKEQLLFAREREKLDKNLSG
                     IKNMGRLPGALFIVDAKKERIAVAEANKLGIPVVAIVDTNADPDLITVPIPGNDDAIR
                     SVALITASLSDVIGEARRQMPMRESSEEAEGTTYSTETGVEAEAEGDRKKKPARRKRR
                     PKPEAIAARMKTDEAPAGGTSAAGGGA"
BASE COUNT          451 a         1055 c          930 g          635 t
ORIGIN      
        1 gaagagcgtg agcacctcga cgccgctctc gatggcacct tccaccactt cgcggaccgc
       61 tttcatgccg gcgcggtgtc ccaggggccg gggcatcaga cgcccgcggg cccagcgccc
      121 gttgccatcc atgatgatgg cgacgtggcg gggcacccca ccgttcaggc gaacctgcgc
      181 caggagatcc gcggtgacca ctagacctcc atgatctccg cttcttttgc cttcaccgcg
      241 tcatcgatgg ctttcatctt ctcatcgtgc agcttctgga cgtccttctc gcagtgcttc
      301 ttctcgtctt ccgacacgtg ctcgagcttc ttggccttgg cgatcgcgtc ggtccgcgcg
      361 tggcggacgc ccacgcgccc ctcctcggcg aacttgtgca cgaccttcac cagctccttg
      421 cgccgctcct ccgtgagggc cggaatcggg atgcggatga ccgtgccgtc actggacggc
      481 gtgaggccga gatcggagac cacgatcgcc ttttcgatgg ccttggcgag cgacttgtcc
      541 catggcgtga cggtgaggag cttgggttcg ggggcggcga cgctcgcgac ctgattgagc
      601 ggcatcatgt tgccgtaggc gtcgacgcgc acgacgtcga ggagcgtagt cgtggctcgg
      661 ccggtgcgca cgctcgagaa ctcgcgcttc accgagtcga ccgacttctc catggccgcc
      721 ttggccgtct tcaacagctc ggggatcgtg ctcatcggac gatggtgccg acgcgctccc
      781 ccgccagaat gcgcgcggtc gcgccggact ggttgatgtt gaataccacg atgggaagcc
      841 ggttctcctt gcacaacccg aaggcgttgg cgtccatcac cgcgtaccct ttcaccagag
      901 catcctggaa ggacagctcg ggaatgaaca tcgccgtctt gtccttcttc ggatcggcgg
      961 tgtagacgcc gtccacgttg gtggccttca tcagcacctg ggcttcgatt tccagggccc
     1021 ggagcaccgc ggcggtatcc gtcgaaaagt aggggtttcc cgtcccgcct gcgaagatca
     1081 ccacccggcc cttgtccagg tgccggatgg cccgccgtcg gatgtacggc tcggccagct
     1141 cttccatccg gatggccgtc atcacccgcg tgtccacacc cttcttctcc agcaattcct
     1201 ggagtgccag ggcgttgatc acggtggcca gcatacccat gtaatcggct gaaacgcggt
     1261 ccatgccctg ctggctggcg gtcgtgccgc ggaggatgtt cccacccccc accaccaggc
     1321 ccagctggac gccagtcgcc gcgaccgcct tcagctcgtc ggtgatgcct tccagggtag
     1381 caaaatcgat gcccgccccg gtgcccttcc cgcccgcgaa atgctcaccc gacagcttga
     1441 gcagcgcgcg cttgtaggtc agggctggca tcggtcagtt ccccccgagc tcgaaccggg
     1501 cgaagcggtg aatgtgcacc ttctcgccgg tcttgccgga gtaggctttt acgagatctc
     1561 cgatggtcac tttgtcgtcc ttcacgaagg gctgctccat gagcacgctc tcctggaaga
     1621 acttcttgag catgccctcg acgatctttg cgcggatctg ctcgggcttc ttttgctcga
     1681 tgacctgggc ctcgtagatg cggcgctcgc gctccacgac gtccgcggga atgtcctcca
     1741 cccggaccgc cagcgggcgc gtcgaagcga tctgcaaggc gatgtcacgc gcgagctgct
     1801 tgaactcgtc cgtgcgcgcc acgaaatcgg tttcgcagtt cagctccacc atcacaccga
     1861 ccttgccggt gaaatggatg tacgcgtcga tcaggccctc ggccgcggtc ttctcggcac
     1921 gcttctccgc cttggcggcg tacttgaccc gcaggatctc ggtcgccttc tcgaggtcgc
     1981 ccccggtctc ctccagggcc ttcttgcaat ccatcattcc ggcaccggtc cggttccgga
     2041 gctccgccac cattttcgct gaaatctcag gcattcttgg tccccgttaa aaaatgccgc
     2101 cctcggccgt caggcacgga gggcggcggc tcttggttca accccgtcgg gcgggcgctt
     2161 acgcgccccc tcccgccgcc gacgttccgc ccgccggcgc ttcgtccgtc ttcatccggg
     2221 ccgcaatggc ctcgggtttc ggccgccgct tccggcgggc cggcttcttc ttgcgatccc
     2281 cttcggcctc ggcctcgacg ccggtctcgg tcgagtacgt cgtgccctcg gcctcctcgg
     2341 acgattcgcg catcggcatc tgccggcgcg cttctccgat gacgtcggac aaggaggcgg
     2401 tgatcagcgc gaccgagcgg atggcgtcgt cgttccccgg gatcggcacc gtgatcagat
     2461 cggggtcggc gttggtgtcg acgatcgcga ccaccgggat cccgagcttg ttggcctcgg
     2521 cgacggcgat gcgctccttc ttggcgtcga cgatgaacag cgccccgggc aggcggccca
     2581 tgttcttgat gcccgacagg ttcttgtcga gcttctcacg ctcgcgggcg aagagcagct
     2641 gctccttctt cgtgtagttc tcgaactcac cgtcggacgc gccctgctcc agatccttca
     2701 agcgcttgat ctgcttcttg atggtctgga agttcgtcag cgtgccgccc agccagcgct
     2761 cggacacgaa gtacgagccg cacttctcgg cctcggcctg cagaatggcc ttgagctgct
     2821 tcttggtgca gacgaagaga acgccctcac ccttcaggac cacgctccgg aggagctcct
     2881 gggcctgcag caactgatgg agagtcttct ggaggtcgat gatgtagatg ccggagcgct
     2941 cggcgaagat gaaccgccgc atcttcgggt tccagcgccg agtctgatgc ccaaaatgga
     3001 caccggcttc cagcaggtcc tgcagttgag gctgcgccat gcaatccttt ccttgggttt
     3061 tagaccgccg t
//
LOCUS       DASUBP010000176        11367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_345515, whole genome shotgun sequence.
ACCESSION   DASUBP010000176 DASUBP010000000
VERSION     DASUBP010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 11367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11367
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345515"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..709)
                     /gene="ricT"
                     /locus_tag="VFU75_06575"
     CDS             complement(<1..709)
                     /gene="ricT"
                     /locus_tag="VFU75_06575"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HEU5466536.1"
                     /translation="MSGQVYEVRFKGRRNEYFSWSAPDPLSLNQAVIVDAERGQDLGR
                     ISALGEIAEKKCTRCGNCGGGPTDPGTSRRRIVRAATTEDVAASEVLRHDEETVRSAA
                     REKVREHNLPMKVTDAEWQWDRKKLTLYFTAEQRVDFRALVRDLASRFHTRIELRQIG
                     ARDEARRLSGVGRCGREYCSASWLPELKPVNLSVAKAQHLSLNPAQISGPCGRLLCCL
                     HYEHDFYVESRKRFPKEG"
     gene            complement(706..1665)
                     /locus_tag="VFU75_06580"
     CDS             complement(706..1665)
                     /locus_tag="VFU75_06580"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HEU5466537.1"
                     /translation="MAVYVLDFERPLLELERQIEELRRAADEAGGDRAKEVESLETKL
                     AELRADIYRQLTPMQRVQVARHPKRPYTLDYLRTVFTDFVELHGDRLFRDDPAIVGGW
                     ARLDGETVMVVGTQKGRDTKENLKRDFGMAHPEGYRKALRLMKLAEKFHAPVITLIDI
                     AGAHPGLGAEERGQASAIATNLIEMAALRTPIIAAVIGEGGSGGALAIGLADRVVMFE
                     NSVYSVISPEGCAAILWKDAAQKERAAEALKLTAQDQLELGVIDEIIPEPTGGAHTDP
                     DAAALSLGDALKRHLRQLRKLKTDKLLARREEKYLSMGVLLEQ"
     gene            complement(1667..5146)
                     /gene="dnaE"
                     /locus_tag="VFU75_06585"
     CDS             complement(1667..5146)
                     /gene="dnaE"
                     /locus_tag="VFU75_06585"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEU5466538.1"
                     /translation="MSFVHLHTHSEYSLLDGANRIPELLDRVQALGMDSLAITDHGNL
                     HGAWAFYNEAKARKVRAILGFEAYLAFGSRKLREKVPDAPASYSHLVLLAKNRTGYKN
                     LIKLSSIGFLEGFYRRPRIDREVLEAHKEGIICLAACLSGEVSLYLRQQNYAQARASA
                     EYFARLFGPDGFFLEVQDHGLPEEKTVAAGMFQLAQDLGLPVVVTNDAHYLMKEDAEA
                     HDVLLAIGTGKDLDDPKRFRFTGSESYVKSEKEMKVLFPNRPELLTETQRIANLCEFD
                     FEKKYFLPQYPRPAEFASDNDLLVHLAQDGAGKRYGSPLPDHVKQRLEYELDVIKRTG
                     YAGYFLIVADLMRAARERGIPVGPGRGSAAGSLVAYSLAITNVDPLKFDLLFERFLNP
                     ERVSMPDIDLDFCFERRGEMIEYVRDRYGRDSVGQIITFGTMKARAAFRDVARTLRMD
                     IGEVDRTTKMIPAGPAFSLTLAEAAEKVPELKALAQSDERVRKVLDLGERIEGLSRHS
                     SVHAAGVVIAPGPLTDYVPVCTAPQDDQAIITQFDMVALEKVGMLKIDFLGLKTLTVI
                     HDAATMVRERKGITINPDDLPLDDPAVYATLRAGRTAGVFQFESPLATDCLRNMKCDR
                     FDDLVAANALLRPGPLDTGMHLVFINRKLGREAVKYPHPALEEVLKPTYGVITYQEQV
                     MRIANVLAGFSLAEADVLRKAVGKKDQALIDAELKRFVERAIANGHAKPVVEELARQI
                     ETFGRYGFNKSHAVAYSVLSYQTAWFKTHHPAEFMAALLSSEIGNTDKVVQYIAEARE
                     LDIDVLPPDVNESGFKFTVVGDKRIRFGLGAVKNVGAGAIASILAGRKDGGPYHSLVD
                     LCDRIDLRLCNKRVIEALIDSGACDSLGGHRAQLIAALERALAEAQVRQGERSAGQGA
                     LFAEAAPSARPGLVLAELPPWTEHDRLAREKAVLGFFISGHPLAKYRTEVELFGTRTT
                     ATLGQWSDQRIRIAAVVTVVKRQVSKKSGNEYARLTLEDFHGTAEALVFPEAWAKLNS
                     TIQADSALLFTGGHSARDRGEEQAPFIVEQAEPLDELRARGAVGLALRWDAGHPPAPE
                     AARAAAALCASHPGSTPLLVEWADGNGGSSEGLGLQVLLRSRSMKVDAAEDLVAALRD
                     LLGSDAVVFRKAS"
     gene            5212..6534
                     /locus_tag="VFU75_06590"
     CDS             5212..6534
                     /locus_tag="VFU75_06590"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEU5466539.1"
                     /translation="MRAVVSLIVVAVMMGLFHHLTAGGLLEARATLALGFLLIAAPLG
                     ADILSRWQLPRLTGYIVIGFIFGPAWLGFVRADEVHTLEFISDAAVALIAFSAGSELI
                     VGELRAQARRLARLAVGGIGLPFVVVTGVILALSPWFPLARHQPIGNVVAIAVVLGTC
                     AAAFSPAATIAIMDETGARGSFARGILNLTVIGDVILMLLLTIVLELARPVAQAGTVD
                     VGAVLTALIGIGASIGAGLVLGWVVSRYLHAVRRDTALFLVVLAVVTAEAARLLSLEA
                     LLIALAAGFYLENFSPVEGERLRAELRRGSRPVYTVFFALAGASVRLDALGEMFPWVV
                     LLVGLRMLSLRMGFRWAGRSPSVEVPFARWGWMGVVSQAGIALGLAMVARRSFPEWGV
                     SLSALIITMIGIHEVVGPILFKRALGAVGELAPTEGPAAAPVASVSTT"
     gene            6531..7382
                     /locus_tag="VFU75_06595"
     CDS             6531..7382
                     /locus_tag="VFU75_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466540.1"
                     /translation="MNRGATALTFLWLFASSLALGQSPTLEGLPASVRSVGLGGVGAA
                     LVGDASVIFTNPAGLATIRHMAFEGSYASIPGGSALSLGAVAIRLGRLDWGAGAQVLS
                     TPGALNHDDAVVASSLVYRRGLMAFGVTGKYAHGPGTPAAGMWAGDVGAALPVFDLFA
                     LGVAVQNIGGDSAGGILPRRVRAGFTMNYVDPEGAYRLLTTIEAQWLAGRHAELVQGL
                     EAGFVLGGVVLVGRVAYGTAALAPGVSKLSAGGGIELGRFHIDYAYRPIDSRGDARHR
                     IGVRWTP"
     gene            7406..8494
                     /locus_tag="VFU75_06600"
     CDS             7406..8494
                     /locus_tag="VFU75_06600"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF021535.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEU5466541.1"
                     /translation="MSRWVLIRRLLLRLALGAAVLIVLALILSPDVRYIARGGWEEAR
                     ILLKRRSLAALVADPKTPPALVQRLKLVLAARAYAADSLRLTAGDTYTTYANVGRDTL
                     LLVLSASRKDKLREVTWSYPIVGTVPYKGFFNFDAARKAAAEQEALGNDVYLRVSDAF
                     STLGYFSDPLLSTAVDRDTMELVATVIHELAHNTLYVPSNTPFDESFANFVGYRGAQA
                     FFRSRGDTIDANRAGARWRDERTLDVFYAELARRLDSAYASPLSGAGLQNTRDDLFGW
                     ARAQLTGPVGQGLETYDWRWFAKAPLNNAVVVAQRLYRLNLNLFDEFYLANNVNLPET
                     IRLIKVRVLTKPGADPYQALYSQSGAGP"
     gene            complement(8495..9766)
                     /locus_tag="VFU75_06605"
     CDS             complement(8495..9766)
                     /locus_tag="VFU75_06605"
                     /inference="COORDINATES: protein motif:HMM:NF012434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase dimerization
                     domain-containing protein"
                     /protein_id="HEU5466542.1"
                     /translation="MSPGTAPVTPPAMSGKTIVQSQFDRAANLVNMEAYMRRILLSPF
                     REMTVDLPVRMDDGRIEVFTGYRIQHNGARGPCKGGIRYHPHADHDEVLGLATIMTWK
                     TALMDIPFGGAKGGITVDPRKLSALELERLTRRFTQRIAIIIGPYRDIPAPDVNTNAQ
                     VMAWILDEYSNRHGYSPAVVTGKPISLGGSLGREEATGRGVMYVMQEYARDFGIPLKG
                     ARVVIQGFGNVGAHLARLLDAETGAKVICVSDVDGGIVNENGLDIKGLLAHVAQRKPV
                     PEWKGGKRITNDEIWKIPCDWLVPAALGSVITKEHNANDVDCKVIVEAANEPTTPTAD
                     LILAERGIPVIPDFLANAGGVTVSYYEWAQNLQQYRWTHEQVNNELKATITKAYAAVR
                     DLAKDKDATFRTAAYAIAIQRVAEAERLRGN"
     gene            complement(9766..10887)
                     /locus_tag="VFU75_06610"
     CDS             complement(9766..10887)
                     /locus_tag="VFU75_06610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5466543.1"
                     /translation="MRTTASFELFPDAPSERAMVRARWLAREGRLSEAESAYREALTT
                     YPDLKPAWAEYFELLRSQKRAADALRLAFGARTQFGEAAFALALEGAALIELDRFREA
                     LEVLEHAVDADPDLAVVWHELGYAAYRIGDRNRALLALDRAFALEPHGATLRLRGKVL
                     RDAGRYQAAEVAFEAAAQSAEHDEQWDEASDEVAITRRYALYAPRKPADLGPPERWFA
                     DTGAVLLASAHSTTAPGDEALVEALIELASDSRWRFGQIVSFGGARDLWIPLSTRLNA
                     PLVSAGGLDAAAVPLVVAPQPLTGDPAWDVAVRAIESRNAGCVFVLEHAGSGPARADV
                     VGVLTNDGVRRPSTPAPGHALTEAQHPAARLASRRLALS"
     gene            10955..>11367
                     /locus_tag="VFU75_06615"
     CDS             10955..>11367
                     /locus_tag="VFU75_06615"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5466544.1"
                     /translation="MDNNRLLAELSEFLRIPSISTLPKHDQDCRRAAEWVAADLRRLG
                     CSDVQLLASETHPVVWGVGPEVPGAPTLVIYGHYDVQPPDPLNEWVTPPFEPTIRDGK
                     LYARGSADDKGQVFCLLRAIEAAGQPRVNLRFLIEG"
BASE COUNT         1686 a         3672 c         3939 g         2070 t
ORIGIN      
        1 tcccttcctt gggaaaacgc ttgcggctct cgacgtagaa atcgtgctca taatggagac
       61 agcagagcag gcggccgcac gggccggaaa tctgtgccgg attgagcgag agatgctgtg
      121 ctttcgcgac cgacaggttc acgggcttga gctcgggaag ccagctggca ctgcagtatt
      181 cgcggccgca gcgccccacg ccggagagcc tccgcgcttc gtcacgagcg ccgatctggc
      241 ggagctcgat gcgggtgtgg aagcgggacg ccaggtcccg caccaacgcc cggaaatcca
      301 ccctttgctc ggccgtgaaa tagagcgtca gcttcttccg gtcccattgc cactcggcat
      361 cggtcacctt catcggcagg ttgtgctcgc gcactttctc gcgggcggcg ctgcggaccg
      421 tttcttcgtc atgccggagg acctcggagg ccgcgacatc ctcggtggtc gcggcgcgga
      481 cgatgcggcg gcgggacgtg cccgggtcgg tgggtccgcc cccgcaattt ccgcaacgcg
      541 tgcacttctt ctccgcgatc tcgccgaggg ccgagatgcg cccgaggtcc tggccgcgct
      601 cggcgtcgac gatgacggct tgattgaggg aaaggggatc aggagcggac catgagaagt
      661 actcgttccg ccgacccttg aacctgactt cgtatacctg gccgctcact gctcgagcag
      721 cacacccatc gagaggtatt tctcttcccg gcgcgccagc agcttgtcgg tcttgagctt
      781 tcggagctgc cggaggtggc gcttcagggc gtcaccgagg gatagcgcgg ccgcgtccgg
      841 gtcggtgtgc gcaccgccgg tcggctcggg gatgatctcg tcgatcacgc ccagctccag
      901 ctgatcctgc gccgtgagct tgagggcttc cgcggcgcgc tctttctgcg ccgcgtcctt
      961 ccacaggatg gcggcgcacc cctcgggcga aatcacggag tagacggagt tctcgaacat
     1021 caccacgcgg tcggcgagcc cgatggcgag cgctcccccc gatccccctt caccgatgac
     1081 ggcggcgatg atgggcgtcc tgagggcggc catctcgatc aggttggtcg cgatggcgga
     1141 tgcctgcccg cgctcctcgg caccgagtcc cggatgagcg ccggcgatat cgatgagggt
     1201 gatgacgggg gcgtggaatt tctccgcgag cttcatcagc cgcagagcct tccggtagcc
     1261 ctcgggatgg gccatgccga agtctctctt gagattctct ttggtgtccc ggcccttctg
     1321 cgtgcccacg accatcaccg tttcgccgtc caggcgcgcc cacccgccga cgatggcggg
     1381 atcgtcgcgg aacagccggt caccgtgcag ctccacgaag tccgtgaaga ccgtgcgcag
     1441 gtagtcgagc gtgtaggggc gcttgggatg ccgggcgacc tgcacgcgct gcatgggggt
     1501 gagctgccgg tagatgtcgg cccggagctc ggcgagcttg gtctcgaggg attcgacttc
     1561 cttggcccgg tcaccgccgg cttcatcggc ggcgcgcctg agctcctcga tctggcgttc
     1621 cagctcgagg aggggacgtt cgaagtcgag tacgtagacg gccatgtcag ctcgcttttc
     1681 taaagaccac cgcgtccgac cccaggaggt cgcgcagggc ggccaccaga tcctcggcgg
     1741 cgtccacctt catgctccgg ctccgcaaca gcacctgcag gccgaggccc tcgctcgagc
     1801 ccccattgcc gtcggcccat tcgaccagga gaggcgtcga gccggggtgc gaggcacaga
     1861 gcgccgcggc ggcgcgggcc gcttccggcg cgggtgggtg acccgcgtcc caccggaggg
     1921 ccaaccccac ggccccacgg gcccggagct cgtcgagcgg ctcggcctgc tccacgatga
     1981 agggcgcctg ctcctcgccg cggtcccgcg cgctgtggcc ccccgtgaag agcagggcgc
     2041 tgtccgcctg gatggtgctg ttcagcttcg cccaggcctc cgggaacacc agcgcttctg
     2101 cggtgccgtg gaagtcctcg agcgtgagcc gggcgtattc attcccgctc ttcttcgaga
     2161 cctgccgttt gacgacggtg accaccgcgg cgatccggat ccgctggtca ctccactgtc
     2221 ccaacgtggc cgtggtccgg gtcccgaaaa gctctacctc ggtgcggtat ttggcgaggg
     2281 gatgcccgga aatgaagaag ccaaggaccg ccttttcgcg tgccaggcgg tcatgctcgg
     2341 tccagggggg gagctccgcg agcaccagcc ccgggcgtgc cgagggggcc gcctcggcga
     2401 acagggcgcc ctggccggct gagcgttcac cctgacgcac ctgtgcttcg gccagtgcgc
     2461 gttccagggc cgcgatcagc tgggcgcggt gaccgccgag tgagtcacag gccccggaat
     2521 cgatcagggc ctcgatcacc cgcttgttgc agagcctgag gtcgatccgg tcgcacaggt
     2581 cgacgaggga gtggtacggg cccccgtcct tcctgccggc gaggatggac gcgatcgcgc
     2641 ccgcgcccac gttcttgacg gcacccaggc cgaagcgaat gcgcttgtcc ccgacgacgg
     2701 tgaacttgaa gcccgattcg ttgacatcgg gcggcagcac gtcgatgtcg agctcgcggg
     2761 cttccgcgat gtactgcacg accttgtccg tgtttccgat ttccgacgac agcagggccg
     2821 ccatgaactc cgccgggtgg tgggtcttga accacgccgt ctggtacgac aacaccgagt
     2881 aggcgaccgc atgggacttg ttgaatccgt accggccgaa ggtctcgatc tggcgggcga
     2941 gctcctcgac gaccggcttc gcgtgtccat tcgcgatggc gcgttcgacg aagcgcttga
     3001 gctccgcatc gatgagggcc tgatccttct tcccgaccgc tttgcggagc acgtcggctt
     3061 cggcgagcga gaagccggcc agcacgttgg cgatccgcat cacctgttcc tggtaggtga
     3121 tgacgccgta ggtgggcttc aggacctctt cgagtgccgg gtgcgggtac ttcaccgcct
     3181 cgcggccgag cttccggttg atgaacacca gatgcattcc ggtgtcgagc ggaccggggc
     3241 ggagcagggc gttcgccgcg accaggtcgt cgaaccggtc gcacttcatg ttccggaggc
     3301 agtccgtggc cagcggcgat tcgaactgga agacgccggc ggtgcgcccg gcccggagcg
     3361 tcgcgtagac ggcggggtcg tccagcggga ggtcgtccgg gttgatcgtg atgcctttgc
     3421 gctcccggac catcgtggcc gcatcgtgaa tcaccgtcag cgtcttgagc cccaggaagt
     3481 cgatcttcag catcccgact ttttcgagcg ccaccatgtc gaactgcgtg atgatggcct
     3541 ggtcgtcctg cggagccgta cagacgggga cgtagtcggt cagcggtccg ggcgcgatga
     3601 ccacgccggc ggcgtgcacg gacgagtgcc gggacagtcc ttcgatccgc tcgcccagat
     3661 ccagcacctt cctgacgcgc tcgtccgact gggccagggc cttgagctcg ggcacctttt
     3721 ccgccgcctc ggcgagcgtc agcgaaaacg cgggcccggc ggggatcatt ttcgtggtgc
     3781 gatccacctc gccgatgtcc atgcgcaggg tgcgggcgac gtcgcggaag gcggcgcgcg
     3841 ccttcatggt tccgaaggtg atgatctgcc cgaccgagtc gcggccgtac cggtcgcgta
     3901 cgtactcgat catctccccc cggcgttcaa agcagaaatc gaggtcgata tcgggcatcg
     3961 aaacgcgttc cggattcagg aagcgctcga acagcagatc gaacttgagg ggatcgacgt
     4021 tggtgatggc gagggagtac gccaccaggg aaccggcggc ggagccacgg cccggtccaa
     4081 ccggaatccc gcgctcccgg gcggcccgca tgaggtccgc gacgatcagg aagtaccccg
     4141 cgtatcccgt ccgcttgatc acgtccagct catactcgag ccgctgcttg acgtgatcgg
     4201 gcagggggga gccgtaccgc tttccggcgc catcctgggc caggtggacc aggaggtcgt
     4261 tgtccgacgc gaattccgcg ggacggggat actggggcag gaagtacttc ttctcgaaat
     4321 cgaactcgca gaggttcgcg atccgctggg tctcggtcag gagctcgggg cggttgggga
     4381 aaaggacctt catttccttc tccgacttca cgtagctctc cgagcctgtg aagcggaacc
     4441 gcttggggtc gtcgagatcc ttgccggtgc cgatcgccag gagcacgtcg tgcgcttcgg
     4501 cgtcctcctt catcaagtaa tgggcgtcgt tggtcaccac caccgggagg cccaagtcct
     4561 gcgccagctg gaacatcccg gccgcgacgg tcttctcctc cgggaggcca tgatcctgca
     4621 cttccaggaa gaacccatcg ggaccgaaca ggcgggcgaa atactcggcg gaggcccggg
     4681 cctgggcgta gttctgctgc ctcaggtaga gggaaacctc gccggacagg caggccgcga
     4741 ggcagatgat gccctctttg tgggcctcca gcacctcgcg gtcgatccgc gggcgccggt
     4801 agaagccttc caggaacccg atggacgaga gcttgatgag gttcttgtag cctgtgcggt
     4861 tcttggcgag gaggaccaga tgcgaatacg aagcgggggc gtcggggact ttctcacgga
     4921 gtttccggga tccgaacgcg agatacgcct cgaatcccag gatggcccgc accttccggg
     4981 ccttggcctc gttgtagaag gcccacgcgc catggaggtt gccgtgatcg gtaatggcga
     5041 gcgagtccat ccccagcgcc tgaacgcggt ccaggagttc cggaatccgg ttggcgccgt
     5101 cgaggaggga gtattcgctg tgggtgtgga gatgaacgaa cgacatcgaa ggaaaattat
     5161 cggcgtggca ccgcattgtc aaaactctcg ccccacggga gattaccgcc gatgcgagcc
     5221 gtcgtcagcc tgatcgtcgt cgcggtcatg atggggctgt ttcatcatct gaccgcggga
     5281 ggtctgctcg aagcgcgcgc cacgctggcg ctgggcttcc tgctcatcgc cgcaccattg
     5341 ggcgccgata tcctctcgcg gtggcagctg ccgcgcctca ccggatacat cgtcatcgga
     5401 ttcatattcg gccccgcctg gctgggattc gtccgggccg acgaagtgca taccctcgag
     5461 ttcatttcag acgccgccgt cgcgctgatc gcgttctcgg ctgggagcga gctgatcgtc
     5521 ggtgagctgc gcgcgcaggc gcgccgactc gcccgcttgg cggtaggcgg gatcggcctc
     5581 cccttcgtgg tcgtgaccgg ggtgattctg gcactgtctc cctggttccc cctcgcccgt
     5641 catcaaccca tcggcaacgt ggtcgcgatc gccgtggtgc tcggcacctg cgcggcggcg
     5701 ttctctcccg ccgcgaccat cgcgatcatg gacgagacgg gagcccgggg atccttcgcg
     5761 cgcggaatcc tgaatctgac cgtgatcggt gacgtcattc tcatgctctt gctcacgatc
     5821 gtgctggagc tggcgcgccc ggtggcgcag gcggggacgg tggacgtcgg cgccgtgctg
     5881 accgcgttga tcgggatcgg ggcttcgatc ggcgccggct tggtcctggg gtgggtggtg
     5941 tcccgctacc tccacgccgt ccggcgtgac accgccctct ttctcgtcgt gctggcggtg
     6001 gtcacggccg aggcggcccg gctgctgagc ctcgaagcgt tgctcatcgc cctcgctgcc
     6061 gggttctatc tcgagaactt ctcgccggtg gaaggggagc gcctgcgggc cgagttgcgc
     6121 cggggctcgc ggccggtgta cacggtcttc ttcgcgctcg ctggtgcgtc ggtgcgcctg
     6181 gatgcgctcg gcgaaatgtt cccctgggtg gtgctcctgg tggggcttcg gatgctcagc
     6241 ctccgcatgg ggttccgctg ggcgggacgc tccccctcgg tggaggtgcc attcgcgcgt
     6301 tggggatgga tgggggtcgt ctcccaggcg ggcatcgcgc tgggattggc gatggtggcc
     6361 cgccggtctt tcccggaatg gggtgtttcg ctttctgccc tcatcatcac gatgatcgga
     6421 atccacgagg tagtgggacc catcctcttc aagcgggcgt tgggggccgt cggggagctc
     6481 gcgccgactg aggggcccgc ggcggcgccc gtggcgtccg tttccacgac atgaatcgcg
     6541 gcgccacggc gctgacattc ctctggctgt tcgcctcctc cctcgcgttg ggtcagtcgc
     6601 ccactctcga gggtcttccg gcctctgtgc gctcggtggg gttggggggt gtgggcgccg
     6661 ccttggtggg cgacgcctcc gtgatcttca ccaatccggc cgggctcgcg acgatccgcc
     6721 acatggcctt cgagggctcc tacgcgtcca tccccggggg atcggccctt tccctcggcg
     6781 ccgtggcgat ccggctcggg cggctcgact ggggcgccgg tgcgcaggtc ctctccactc
     6841 ccggcgcgct caatcacgac gatgccgttg tagcatcctc tctggtgtac cggcgcggcc
     6901 tcatggcctt cggggtgacc ggaaaatacg cccacgggcc cggaacgccg gcggccggga
     6961 tgtgggcggg ggatgtcggc gcggcgctgc cggtgttcga tctcttcgcg ctgggcgtcg
     7021 cggttcagaa catcggcggg gattcggcgg gcggcatcct gccgcgccgg gtacgggccg
     7081 ggtttaccat gaactacgtg gatccggagg gggcgtaccg cctgctcacc accatcgaag
     7141 cccagtggct ggcggggagg catgcggagc tggtgcaggg gttggaggcg gggttcgtgc
     7201 tcgggggggt cgtcctggtg gggcgggtgg cgtacgggac ggcggcgttg gccccggggg
     7261 tctcgaaatt gagcgccggt gggggcatcg agctcggccg tttccatatt gattatgcgt
     7321 accggccaat cgacagccgg ggggatgcac gacacaggat cggggtgcga tggacgccgt
     7381 agtggtcgac cgacgccggg gagggatgag ccggtgggta ctgatcagga ggctgctcct
     7441 caggctggcc ctgggtgccg ccgtgctgat cgtcctggcg ctgattcttt cccctgatgt
     7501 ccggtacatc gcgcggggcg gatgggagga ggcgaggatc ctgctgaagc gccggtccct
     7561 cgccgcgctc gtcgccgatc cgaagacgcc gccggcgctg gtacaacggc tcaagctcgt
     7621 cctggccgcc cgcgcctacg ccgccgattc gctccgtctc accgccggtg acacctacac
     7681 gacctacgcg aatgtcggcc gggacaccct gcttctggtg ctctccgcgt ctcgcaagga
     7741 caagttgcgg gaggtgacgt ggagctatcc gatcgtcggc accgtgccct acaagggctt
     7801 tttcaacttc gacgcggcgc ggaaggcggc ggcggagcag gaggctctcg ggaacgacgt
     7861 gtatctgcgg gtgtcggatg cgttctccac gctggggtat ttctcggatc ccttgttgtc
     7921 gacggcggtc gaccgggaca ccatggagct cgtggccacc gtcattcacg agctggctca
     7981 caatacgctc tatgtcccga gcaacacgcc gttcgacgaa agcttcgcga atttcgtggg
     8041 gtatcgcggg gcccaggcgt ttttccggtc gcggggggac actatcgatg ccaaccgtgc
     8101 gggggcgcgt tggcgggacg agcggaccct cgacgtcttc tacgccgagc tggcccggcg
     8161 gctcgattcg gcgtatgcct ctccgctttc gggagccggg ttgcagaaca cccgggacga
     8221 tctctttggg tgggcgcggg cgcagctgac gggaccggtg gggcaggggc tggagacgta
     8281 cgactggcgg tggtttgcga aggcaccgct gaacaacgcc gtggtggtgg ctcagcgact
     8341 ctaccggctg aatttgaacc tgttcgatga gttctatctg gcgaacaacg tcaaccttcc
     8401 agaaaccatc cggttgatca aggtgcgggt cctcaccaag ccgggagcgg atccctatca
     8461 ggcgctctat tcgcagagtg gtgccgggcc gtagtcagtt tccccgcagc cgctccgctt
     8521 ccgccacccg ctgaatcgcg atcgcgtacg ccgcggtcct gaaggtcgca tccttgtcct
     8581 tggcgagatc gcgcacggcc gcgtacgcct tggtgatcgt cgctttgagc tcgttgttca
     8641 cctgctcatg ggtccagcga tactgctgaa ggttctgcgc ccactcgtag tagctcaccg
     8701 tcaccccacc cgcgttcgcc aggaagtccg gaatcaccgg aatcccccgc tcggccagga
     8761 tcaaatcggc cgtcggggtg gtcggctcgt tggcggcctc gacgatgacc ttgcagtcca
     8821 cgtcattcgc gttgtgctcc ttggtgatca cgctgccgag cgcggcgggc acgagccagt
     8881 cgcaggggat cttccagatc tcgtcattcg tgatccgttt gccgcccttc cactccggca
     8941 cgggtttccg ctgcgccacg tgtgccagca ggcctttgat gtccagccca ttctcgttca
     9001 cgatgccgcc atccacgtcg ctcacgcaga tcaccttcgc gccagtctcc gcatcgagga
     9061 ggcgggccag gtgggcgccg acgttgccga agccctggat gacgacgcgc gcgcccttga
     9121 gggggatccc gaaatcgcgg gcgtattcct gcatcacgta catcacgccg cgcccggtcg
     9181 cctcttcgcg gccgagcgat ccgccgaggg aaatcggctt gcccgtgacc accgcgggcg
     9241 agtacccatg gcggttcgag tactcatcca ggatccaggc catcacctgc gcgttcgtgt
     9301 tgacgtcggg cgccgggatg tcgcggtagg gaccgatgat gatggcgatg cgttgcgtga
     9361 agcggcgggt gaggcgttcc agctcgaggg ctgaaagttt gcggggatcg acggtgatcc
     9421 cgcccttggc acccccgaag gggatgtcca tcagcgccgt cttccaggtc atgatggtgg
     9481 cgaggcccag gacctcgtca tgatccgcgt gggggtggta gcggatcccg cctttgcagg
     9541 gtcctcgcgc gccattgtgc tggatccggt atccggtgaa cacctcgata cggccgtcat
     9601 ccatgcgcac cggcaggtcc accgtcatct cccggaaggg agagagcagg atccgccgca
     9661 tgtacgcttc catgttgacc aggttggcgg cgcggtcgaa ctgactctgg acgatggtct
     9721 tccccgacat tgccgggggt gtgaccggcg cggtccccgg actcatcagg agagggccag
     9781 ccggcgcgag gccaggcggg ccgcggggtg ctgcgcttcg gtaagcgcgt gaccgggggc
     9841 gggagtactg gggcgacgga cgccatcatt cgtcagcact ccgaccacgt cggcgcgcgc
     9901 ggggcccgag ccggcgtgtt cgagcacgaa cacgcacccg gcgttccggg actcgatggc
     9961 ccgcacggct acgtcccatg cggggtcacc cgtcaggggc tgaggcgcga cgacgagggg
    10021 gacggcggcg gcatcgaggc cgccggcact cacgagcggg gcgttgaggc gggtggaaag
    10081 gggaatccag agatctctgg cgccgccgaa gctcacgatc tgtccgaagc gccaccggga
    10141 atccgaagcc agctcgatca gcgcttcgac cagtgcctca tctccaggag cggtagtcga
    10201 atgggcggag gccagcagca cggcgcccgt gtccgcgaac caccgttccg ggggacccag
    10261 gtcggcgggt ttccgtggag cgtacagcgc gtagcggcgg gtgatggcga cctcatcgct
    10321 cgcctcgtcc cactgctcgt cgtgctcggc cgattgcgcc gccgcttcga acgcgacctc
    10381 ggcggcctga tagcggcccg cgtcgcggag caccttcccg cgaagacgca gcgtggcgcc
    10441 atgcggctcg agtgcgaacg cccgatcgag tgcgaggagg gcccggttgc ggtcaccgat
    10501 gcggtaggcg gcgtagccga gctcgtgcca gacgaccgcg agatcggggt cggcgtcgac
    10561 ggcgtgctcc aggacctcga gggcctcccg gaaccggtcg agctcgatga gcgcggcacc
    10621 ttcgagcgcc agggcgaacg cggcctcgcc gaattgggtc cgggctccga aggcgagccg
    10681 gagcgcgtcg gccgcgcgtt tctgggaccg taagagctca aagtattcgg cccaggcggg
    10741 cttcaggtcc ggatacgtcg tgagggcttc gcggtacgcg gattcggctt ccgaaagccg
    10801 gccttcgcgg gccaaccatc gcgcgcggac catggcacgc tccgaagggg cgtcagggaa
    10861 caactcgaat gacgcggtgg tccgcattcg cgagaatcta cccccagcag ggccgctggc
    10921 tagtcgactc ggcgccttct agttttccgg caccatggac aacaaccgcc tgctcgccga
    10981 gctctcggaa ttccttcgga ttccgtcgat ttccacgttg ccgaagcatg accaggattg
    11041 ccgccgtgcc gcggaatggg tcgccgcgga cctgcgacgt ctcgggtgca gtgatgtgca
    11101 actgctggcg agcgaaacgc atccggtggt ttggggtgtc ggccccgagg ttcctggggc
    11161 gccgacactg gtgatttacg gtcactacga cgtgcaaccg cccgatccgc tgaacgagtg
    11221 ggtcacgccc ccgttcgaac cgacgattcg agatggaaag ctctacgcgc gcggcagcgc
    11281 cgacgacaag ggacaggtgt tctgtctcct gcgcgccatc gaagccgccg gtcagccacg
    11341 tgtcaatctc cggttcctga tcgaggg
//
LOCUS       DASUBP010000177         6381 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_972228, whole genome shotgun sequence.
ACCESSION   DASUBP010000177 DASUBP010000000
VERSION     DASUBP010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6381)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6381)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6381
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_972228"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..204)
                     /gene="pyrG"
                     /locus_tag="VFU75_06620"
     CDS             complement(<1..204)
                     /gene="pyrG"
                     /locus_tag="VFU75_06620"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683113.1"
                     /note="CTP synthase; cytidine triphosphate synthetase;
                     catalyzes the ATP-dependent amination of UTP to CTP with
                     either L-glutamine or ammonia as the source of nitrogen;
                     in Escherichia coli this enzyme forms a homotetramer;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthetase"
                     /protein_id="HEU5466545.1"
                     /translation="MSSTKYVFVTGGVVSSLGKGIAAASLGRLLVERGLKVTIQKFDP
                     YINVDPGTMSPFQHGEVYVTDDGA"
     gene            complement(201..950)
                     /gene="kdsB"
                     /locus_tag="VFU75_06625"
     CDS             complement(201..950)
                     /gene="kdsB"
                     /locus_tag="VFU75_06625"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: protein motif:HMM:NF003952.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HEU5466546.1"
                     /translation="MPLLGVIPARLGSSRLPKKPLLPLAGDPLILWVVRRVAELELCD
                     RLVVATDAREISSVVEGAGFEAVMTSPKHESGTERIAEVIANKEFKSFDLILNVQGDE
                     PLVSPQALTGTVSRLEQGDPIGTAAGSLDPALAADPSRVKVVVGPDGRAAYFSRAPIP
                     FDRDGTGTVVYHQHVGVYGYTREALERWVKLPPVPEERWERLEQLRPLLHGVPIGVSL
                     FDGPAAPGVDTADDLAFAEREITRHLKEVNS"
     gene            complement(982..1572)
                     /locus_tag="VFU75_06630"
     CDS             complement(982..1572)
                     /locus_tag="VFU75_06630"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEU5466547.1"
                     /translation="MLLVLDNYDSFTYNLVQYFGELGAEPVVYRNDALSVEDVVRLKP
                     SALVISPGPGTPAQAGIAVPLIRALAGQTPILGVCLGHQAIGEAFGGKVVRADRLMHG
                     KTCEVVHDEDELFEGTPSPMTAMRYHSLVVAPEGLPRDLVVTAWSADRPRAEEIMAMK
                     HRQFPVYGVQFHPESVGTEFGKLLLGNFLRVAQAMV"
     gene            complement(1578..1847)
                     /locus_tag="VFU75_06635"
     CDS             complement(1578..1847)
                     /locus_tag="VFU75_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466548.1"
                     /translation="MSAMSRRLTVRLLTVALPLALVATAAFKVGEYVQGQPHMEAAMT
                     ALKSAKMHLGEAADDKGGHKVAALKAVNEAIEHVQAGIDYAKANH"
     gene            complement(1938..2420)
                     /locus_tag="VFU75_06640"
     CDS             complement(1938..2420)
                     /locus_tag="VFU75_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466549.1"
                     /translation="MSDDRARRTLLRHALATLAYRGAKAFRDAPEGFVDFRAAPGSRS
                     AGEILAHIGDLLDWGLGLAKGQHKWKQSPVLPWTEGVARFHTSLERFDTALGSDDGLG
                     FSAEQLFQGPIADSFTHVGQIAMLRRLASAPIKGEDYSRADIAAGRVSQAQHVPKVEF
                     "
     gene            complement(2417..2788)
                     /locus_tag="VFU75_06645"
     CDS             complement(2417..2788)
                     /locus_tag="VFU75_06645"
                     /inference="COORDINATES: protein motif:HMM:NF021612.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase-like protein"
                     /protein_id="HEU5466550.1"
                     /translation="MTTLIAVLSTFVALAALFLWWKGRYTRAVRQDAIRRSLAVVTGK
                     VSEQLVPHFPQFPFAPRDARFLGSPVDFVVFDGLSGGEGAVRRVVFVEVKTGEARLSP
                     RERGVREAIERGAVEWMELRV"
     gene            2839..5184
                     /locus_tag="VFU75_06650"
     CDS             2839..5184
                     /locus_tag="VFU75_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HEU5466551.1"
                     /translation="MRPSVFALLFAPVLLAAQQPYAPTTLSAEDYARAEKFMGYNVNP
                     LVFNGAVRGTWLPADRFWFRNQSAQGFEYLVADPARKSLKPAFDQAAIAKALSAAADT
                     TYEAMHLPFPDFSYGAEAKSISFDAGRSHWTCTLEPAKCTGERRQRELGPRTEVLSPD
                     GKRAAFIKSDNLWVRDVATGKETQLTTDGAPDFGYATDNAGWTKSDRPILAWSPDSRK
                     IATFQQDQRRVGDMYLVQTRVGHPVLEAWKYPLPGDSVVSMIQRVIIDVDQAKVIRLQ
                     MVPDQHRSTLCDDIACGQSWTDVSWSPDATKLFFVSTSRDHKQEWVRVADAATGAVRE
                     VFNESVPTQYESGFRGSNIRALWGSNEFIWFSERDNWGHLYLYDLGTGKLKNRITQGE
                     WPVLDIVRLDEATRTVWFESAGHETQNPYFRQLWSVRLDGSRLTNLTPEVGDHDVALS
                     PDGKYFVDVYSQPDVPPVSVLRDNTGKVVMPLEKADISKLVASGWKPPMVISVKARDG
                     KTDLYGLLFRPTNMDTTRKYPIVNNIYPGPQIGSVGGWGFAAAHGDAQGMAELGFVVV
                     QINSTGTPMRSKSFHDAYYGNMGDNGLPDQITGEQQLAKRYSWIDLDRAGIWGHSGGG
                     FASTDGILRYPDFYKVAVSEAGNHDNREYEDDWGERYQGLETWSGGKSNYDDQANQNM
                     AKNLKGKLLIAYGTTDNNVPPYNSLNLINALIDANRDFDELSLPNRRHGFGNEPYMVR
                     RRWDYFVKNLLGATPPTEYQMHPPATPGFRRAVAGAADDEQ"
     gene            complement(5233..5769)
                     /locus_tag="VFU75_06655"
     CDS             complement(5233..5769)
                     /locus_tag="VFU75_06655"
                     /inference="COORDINATES: protein motif:HMM:NF019614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HEU5466552.1"
                     /translation="MNHIALIRGVNVGGRSMIAMADLRTACENLRFKNPRTLLNSGNL
                     VVESPDSPAQVESRLEKGMEKLLGRELEFFVRSPKEWDAIIKANPYAKEATTDPGRLV
                     LLCFKDKVTPAAVKDLQKKIPGRETLEARGREGYAYYPDGQGQSKFTGALIERTLGTK
                     CTARNWNTVLKLAAMAKE"
     gene            complement(5766..6146)
                     /locus_tag="VFU75_06660"
     CDS             complement(5766..6146)
                     /locus_tag="VFU75_06660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466553.1"
                     /translation="MIARRLTGAFCAVSVPLLQACGAGWHRTDIVTPESPRQQVEVWQ
                     GQASQQWHAVQVTDSTVSGISFVKPVECDTCRVSVPRATVDSIRLGNPTLGFWKSFGL
                     VLLAPVAFLFAVCGPSMCADLGRD"
     gene            complement(6143..>6381)
                     /locus_tag="VFU75_06665"
     CDS             complement(6143..>6381)
                     /locus_tag="VFU75_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466554.1"
                     /translation="EVRSRDGLQRGLALIHYADGSVHILGAGQNVGDGKDEIRDWSVF
                     YLRHHKAAIWVSRAFAPGGSLTWDGKVYRWASGQ"
BASE COUNT         1190 a         2144 c         1996 g         1051 t
ORIGIN      
        1 ggcgccgtcg tccgtgacgt agacctcgcc gtgctggaag ggcgacatgg tcccggggtc
       61 gacgttgatg tacggatcga acttctggat cgtcaccttg agtccgcgct cgaccaggag
      121 tcgccccagg gaggccgccg caatgccctt ccccagagag ctcaccacac ctccggtgac
      181 gaagacgtac ttggtcgagc tcacgaattc acctctttca gatgccgggt tatttcgcgc
      241 tccgcgaacg cgagatcgtc cgccgtgtcg actcccggcg cggccgggcc gtcgaagagc
      301 gagacgccga tggggacgcc gtgcaacagg ggccgcagct gctccagacg ctcccagcgt
      361 tcctcaggaa cgggagggag cttgacccac cgctcgagcg cttcgcgagt atatccgtag
      421 actcccacgt gctggtggta gacgacggta ccggtcccgt cacgatcgaa ggggatgggg
      481 gcccgcgaga agtaggcggc ccggccatcg gggcccacca ccaccttcac cctgctgggg
      541 tcggcagcga gcgcggggtc gagggagccc gcggcggtgc cgatggggtc cccctgctcc
      601 agtcgggaga ccgtcccggt gagtgcctgg ggggaaacga ggggctcgtc cccctgtaca
      661 ttcaggatga ggtcaaacga cttaaactct ttattagcaa taacttcagc gattctctcg
      721 gtgccactct cgtgcttcgg cgaggtcatc accgcttcga atccagcccc ctccacgacg
      781 gacgaaatct cccgcgcgtc ggtcgccacg accaggcggt cgcagagttc gagctcggcg
      841 acacgtcgga ctacccagag gatgaggggg tcgcctgcca aaggcaggag gggcttctta
      901 ggcagtcggc tggagcccag ccgagcggga atcacaccga ggaggggcac agggcaagat
      961 acaagagtcg tgccgtggag tctagaccat ggcctgtgcc accctcaaga agtttcccaa
     1021 caagagttta ccgaattcgg tgcccaccga ctccggatgg aattgcacgc cgtagacggg
     1081 gaactgccgg tgcttcatgg ccatgatctc ttcggcccgg ggccgatcgg cactccaggc
     1141 ggtcaccacc agatctcgag gcagaccctc gggggccacc accagcgagt ggtagcgcat
     1201 cgccgtcatc ggtgacggcg tcccctcgaa caactcgtct tcgtcgtgca ccacctcaca
     1261 ggtcttcccg tgcatcaggc gatcggcgcg cacgactttg ccaccgaacg cttctccgat
     1321 cgcctgatgt ccgagacaca cacccaggat gggcgtctgc cccgcgagtg cacggatcag
     1381 cgggaccgcg atcccggcct gcgcgggcgt gccgggcccc ggcgatatca ccagggccga
     1441 cggcttcagc cgcaccacgt cctcgaccga caacgcgtcg ttgcggtaga ccacgggctc
     1501 cgcccccagc tccccgaaat actggacgag gttgtaggtg aaagagtcgt agttgtcgag
     1561 aacgaggagc atactggtta gtgattcgcc ttggcgtaat cgatgccggc ctgcacgtgc
     1621 tcgatcgctt cgttgacggc cttcaaggcg gcgaccttgt ggccaccctt gtcgtcggcc
     1681 gcttcgccga gatgcatctt ggcgctcttg agcgccgtca tcgcggcttc catatgcggc
     1741 tgaccctgga cgtactcgcc cactttgaag gcggcggtcg cgaccagcgc cagcggcagc
     1801 gccacggtaa gcaggcgaac ggtgagacga cgggacatgg cggacatggg acctccggaa
     1861 atggtgtgta gcagcatgga acagggcggc ttccacttca acatgccccc gggccgatgg
     1921 gcccgccagc gccctgacta gaactccacc ttcgggacat gctgggcctg gctcacccgg
     1981 ccggcggcga tgtcggcgcg ggaatagtcc tcgcctttga tcggtgcact cgccaggcgg
     2041 cgcagcatcg cgatctgccc gacgtgggtg aaggaatcgg cgatcggccc ctggaagagc
     2101 tgttccgccg aaaaccccag accatcatca ctccccagcg cggtatcgaa acgctcgagg
     2161 gaggtgtgga agcgtgccac cccctccgtc cagggcagca ccggcgactg cttccacttg
     2221 tgctgcccct tggcgagacc cagtccccag tccaggagat ccccgatgtg cgcgaggatt
     2281 tccccggcgg accgggaccc cggcgcggcc cggaaatcga cgaatccctc cggagcatcc
     2341 cggaacgcct tcgctccccg gtacgcgagg gtcgcgaggg cgtgacgcag gagggtgcgg
     2401 cgggctcgat cgtcgctcat acgcggagct ccatccattc cacggcgccc cgctcgatcg
     2461 cttcgcggac gccgcgctca cggggtgaca ggcgcgcctc tcccgtcttc acttccacga
     2521 ataccacccg ccggaccgcg ccttctcccc ccgacaggcc gtcgaacacg acgaaatcta
     2581 ccgggcttcc cagaaagcgc gcgtcccgcg gcgcgaaggg aaattgcgga aaatggggaa
     2641 ccagctgctc ggagaccttg ccggtgacca ccgccaggga ccggcggatc gcatcctgcc
     2701 ggaccgctcg cgtataccgg cctttccacc acaggaacag cgccgcgagg gccacgaacg
     2761 tagagagaac ggcaatgagc gtggtcatgg accagaatat atattggccg tccttcaccc
     2821 tttccgagat cccgatctat gcgaccgtcg gttttcgccc tcctgttcgc tcccgtactc
     2881 ctcgccgcgc agcaacccta cgcccccact accctctccg ccgaggacta cgcacgggcg
     2941 gagaaattca tgggctacaa cgtcaatccg ctggtcttca acggggccgt ccgcggcacc
     3001 tggctcccgg ccgaccggtt ctggttccgg aatcagtccg cgcaagggtt cgagtacctc
     3061 gtcgctgatc cggcccggaa gtccctgaaa ccggccttcg accaggcagc catcgccaag
     3121 gccctgtccg ccgccgcgga tacgacgtac gaagcgatgc atctgccctt ccccgacttc
     3181 agctacggcg cggaagcgaa gtccatcagc ttcgacgcgg gccggagcca ttggacctgc
     3241 accctggagc ccgcgaagtg caccggcgag cgccggcagc gcgaattggg gccgcgaacc
     3301 gaggtgctct ctcccgacgg caagcgtgcc gccttcatca agagtgacaa tctctgggtg
     3361 cgggacgtcg cgacggggaa ggaaacgcag ctcaccaccg acggcgcgcc cgacttcggg
     3421 tacgccacgg acaatgccgg ctggaccaag agtgaccgcc cgatcctggc ctggtctccc
     3481 gattcacgga agatcgcgac cttccagcag gatcagcgcc gggttggtga catgtacctc
     3541 gtccagaccc gcgtcggaca cccggtgctc gaagcctgga agtatcccct gcccggcgac
     3601 tccgtggtca gcatgatcca gcgcgtcatc atcgacgtgg atcaggcgaa ggtgatccgg
     3661 ctccagatgg ttcccgatca acaccggtcg acgctgtgcg acgacatcgc ctgcggccaa
     3721 tcctggaccg acgtgagctg gagtcccgat gccacgaagc tgttcttcgt ctcgacgtcg
     3781 cgggaccaca agcaggagtg ggtgcgcgtc gccgatgcgg cgaccggcgc cgtgcgcgaa
     3841 gtgttcaacg agtcggtccc gacccagtac gagtccggat tccggggatc gaacatccgc
     3901 gccctctggg gctccaatga gttcatctgg ttctcggagc gggacaactg gggacacctg
     3961 tacctctatg acctgggcac cggcaaactg aagaaccgga tcacccaggg cgagtggccg
     4021 gtcctcgaca tcgtccggct cgacgaggcg acccgcaccg tatggttcga atccgccgga
     4081 catgagacgc agaacccgta tttccggcag ctgtggagcg ttcgactgga cggctcccgg
     4141 ctcacgaacc tgacgcccga agtgggcgac cacgatgtgg cactctcgcc cgacgggaag
     4201 tatttcgtgg acgtttactc ccagcccgac gtgcctcccg tgtccgtcct ccgcgacaac
     4261 accggcaagg tggtgatgcc gctcgagaag gccgacatct ccaagcttgt ggcctccggg
     4321 tggaagccgc cgatggtgat ttcggtgaag gcgcgcgacg gcaagaccga tctgtacgga
     4381 ctgcttttcc gtccgaccaa catggacacg accaggaagt atccgatcgt gaacaacatc
     4441 taccccgggc cgcagatcgg cagcgtgggg ggctggggat tcgccgcggc ccatggcgac
     4501 gcgcagggca tggcggagct gggcttcgtg gtggtgcaga tcaactcgac gggaacgccg
     4561 atgcgctcga agtctttcca tgacgcgtac tacggcaaca tgggagacaa cggactcccc
     4621 gatcagatca cgggcgagca gcagctggcc aagcggtact cgtggatcga tctagaccgc
     4681 gccggcatct gggggcattc cgggggcgga ttcgcctcga ccgacggcat cctgcgctac
     4741 cccgatttct acaaggtggc ggtgtccgaa gccggcaacc acgacaaccg cgagtacgaa
     4801 gatgattggg gcgagcgcta ccaaggactc gagacgtgga gtggtggcaa atccaactat
     4861 gacgatcaag ccaatcagaa catggccaag aacctgaaag gcaagctcct gatcgcctac
     4921 gggaccacgg acaacaacgt gcccccgtac aactcactga acctgatcaa tgcgctcatc
     4981 gacgccaacc gcgacttcga cgagctgtca ctgcccaacc gccggcacgg gttcgggaac
     5041 gagccctaca tggtccgccg ccgctgggac tatttcgtca agaacctcct cggcgccacg
     5101 cccccgaccg agtatcagat gcatccgccc gcgaccccag gcttccgccg ggccgtggcg
     5161 ggggccgcag acgacgagca gtagggggag tcgatgcccc cgggcgcaag cccgggggtg
     5221 agggagtccg gactactctt tcgccattgc cgccagcttg agcacggtat tccaattgcg
     5281 cgccgtgcac ttggtgccca gcgtccgttc gatgagggct ccggtgaact tcgactgtcc
     5341 ctggccgtcg ggatagtagg catagccttc ccggccccgg gcctcgagcg tttccctccc
     5401 cggaatcttc ttctgaagat ccttcaccgc cgccggcgtg accttgtcct tgaagcacaa
     5461 caggaccagt cgacccggat ccgttgttgc ttccttggca tagggattcg ccttgatgat
     5521 ggcgtcccac tctttgggag agcggacgaa gaattcgagc tcccgaccca ggagcttttc
     5581 catccccttt tccagccgcg actcgacctg agcgggggag tccgggctct cgacgaccag
     5641 attcccgctg ttcaacagcg tgcgggggtt cttgaaacga aggttctcgc acgcggtgcg
     5701 cagatcggcc atcgcgatca tgctcctgcc gcccacattg acgccccgga tcagcgcgat
     5761 gtgattcaat cgcgcccgag gtccgcacac atggaaggtc cgcaaacggc gaagaggaag
     5821 gcgaccgggg ctaggaggac aaggccgaaa gacttccaga agccgagtgt cgggttgccc
     5881 agacggatcg agtctactgt ggcccgcggg accgagacgc ggcaggtatc acactcgacg
     5941 ggcttcacga acgagatccc ggacacggtg gaatccgtca cctgaacggc gtgccactgc
     6001 tgcgaggcct gcccttgcca cacttcgacc tgctgacggg gggactcggg tgtgacgatg
     6061 tccgtgcggt gccacccagc cccgcaagcc tgcaggaggg gcaccgagac ggcacaaaac
     6121 gcgccggtca gccggcgggc gatcactgac ccgaggccca gcggtagacc ttgccgtccc
     6181 atgtgagcga ccctcccggg gcgaacgccc ggctcaccca gatcgccgcc ttgtggtggc
     6241 gcaggtagaa cacgctccag tcccggatct cgtccttccc gtccccgaca ttctgaccag
     6301 cgccaagaat atgaacggag ccgtcggcgt aatgaatcaa cgcgagtccc cgttgcaatc
     6361 catctcggct cctgacctcg a
//
LOCUS       DASUBP010000178         4590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1081673, whole genome shotgun sequence.
ACCESSION   DASUBP010000178 DASUBP010000000
VERSION     DASUBP010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1081673"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..1005)
                     /gene="ispE"
                     /locus_tag="VFU75_06670"
     CDS             complement(118..1005)
                     /gene="ispE"
                     /locus_tag="VFU75_06670"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:TIGR00154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEU5466555.1"
                     /translation="MNPDQLRVAAHAKVNLFLRILAREHAGYHQIETAFLLLELADQI
                     EVRRTDRGVKLEVDGPDLGPADENLAVRAAKAVLEATGAKFGVEIALTKKIPTRAGLG
                     GGSSDAAATLHAVNALAGNAVPRHELNHLAAKLGSDVAFFAAGVPAALGWGRGERLLA
                     LTPPPAAPALVALPRGVEVATPDAYRWWDQSGMHDGSRGPVVLEPDSLSSWGSVGRLG
                     ANDFELPVFGKHPEIRTVFERMAETRPYWVRMSGSGSAVAAVYRNERDRDDAVARLGN
                     RDQTTIATSTRARPPAPLA"
     gene            complement(1002..2063)
                     /locus_tag="VFU75_06675"
     CDS             complement(1002..2063)
                     /locus_tag="VFU75_06675"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEU5466556.1"
                     /translation="MKSAILSTSPSTPPKRPASWAWLLGLAITIACLWWAVRGVNWAD
                     FGAALRTVNVPLLLLVVVIATCTFPLRMIRWRVMLRGDDDAPIAWSALWHAVAIGFMA
                     NNLLPARAGEFARAYVVKRQAPVRFITALATIGVERLFDGLFMLALMAVAIAAPSFPA
                     NTLVEGVSLAHVARGAAIVFSGFLVVAFVVALHPAPWLRLADRLTHAALPERVAHRVN
                     DLLHGLIEGLAVLRKPGRFIAVLWWSLVQWLVNAASFALCFKAFGLSVPAVGSFLLQG
                     ILGFGVAIPSSPGYAGVFETATKVTLELYHVDPSRALAYALTYHLTTFIPVTALGLYS
                     LSRARLHLGDLRGARADTA"
     gene            complement(2020..3771)
                     /locus_tag="VFU75_06680"
     CDS             complement(2020..3771)
                     /locus_tag="VFU75_06680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466557.1"
                     /translation="MSSSTPPTGPHRMTDFFTLEAGEYLERLDGALAGGTTPPPVDEM
                     LKLSRALRGSALMANKGLIARAAAGLEALVRAVREGRRNWDAETQQITIRAVDDLKIL
                     IRSVLNWTDADSVKAEALSGELERLAGTPRASGAVPKMDAPSLDAGARAFVAREGAAI
                     ASALDRASQALRANPGGRDPLHLVTKALQPLRGLAALNDLPPLPDVLEGIERAIAELD
                     RNEGAPSPGVDELFQTAASAIAGAAREVAEQGRPDPEGATFKAFAAKLVAFLGSKEAE
                     PGVVPIASLYYADDGPHVVKRGTFSARPATLGKLELVSHGEHLRQAADSLERAPSATQ
                     RELRAHTLAGTFRALMNEGGGPLAAGVSEFAEAARDAVRDGAAVREAARFSAELRRAG
                     DILTRSSTEEEGSLATELADVTAKIKGLGASPASPVPVVPIESLAPEPSAPAPAPTPT
                     PAPAPPVEDSIEETPDLVGTWAMYERVTAGELGEASLDVLIAGGTAVAPPPVAVRAAP
                     APPPPPASKPAPAPAPAPAPVEQQVVDIRTLLYRGDAARRRAAELRAHARNVSGEELR
                     ALVDEVCDLVDLALDPS"
     gene            complement(3825..4577)
                     /locus_tag="VFU75_06685"
     CDS             complement(3825..4577)
                     /locus_tag="VFU75_06685"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HEU5466558.1"
                     /translation="MRLYVNIDHVATIREARKTDEPDPVRAAADALAGGADGITVHLR
                     EDRRHIQDADVKALAARPGVVLNLELAASPEIVRIAARLRPHQATLVPERRQEITTEG
                     GLALKPRDRRLAGAISRLQDADIRVSLFIDPDPRTIDRAAGLGVPAVELHTGRYAHTW
                     RKNARALVQLQKASRHARETGLAVHAGHGLTYLNVRPVAAIPEIEELNIGHSIVSNAV
                     FWGLEGAVRRMRELVDGAISHQPSAVSKRADG"
BASE COUNT          708 a         1553 c         1650 g          679 t
ORIGIN      
        1 ggtcatgctg gtagccccgg gtccggtccc atcgctgcag gccgccacga tgatgactgc
       61 caggaggagc tttctcatac accgacatac tgcgcatacc gcacaaccca gtcaaggtca
      121 ggccagggga gccggcggcc gcgctctggt gctggtcgcg atggttgtct ggtctctgtt
      181 gcccagcctc gcgacggcgt cgtcccggtc gcgctcgttc cggtacaccg cggccaccgc
      241 gcttcctgac cccgacatcc gcacccagta cggcctcgtc tcggccatcc gctcgaacac
      301 ggtcctgatc tcaggatgct tgccgaatac cgggagctcg aagtcattcg cgccgagccg
      361 tcccacgctc ccccagctcg acagcgaatc gggctcgagc acgacgggcc cccggctccc
      421 gtcgtgcatt ccactctgat cccaccaccg gtacgcgtca ggtgtcgcca cctccacgcc
      481 tcgaggcagg gcgaccaggg cgggagccgc aggcggtgga gtcagcgcca gcaggcgctc
      541 gccacgcccc cagcccagag ccgcgggcac gccggccgcg aagaacgcga cgtccgaccc
      601 gagcttggcg gcgagatgat tcagctcgtg ccgcgggacc gcattgccgg cgagggcatt
      661 gacggcatgg agcgtggcgg cggcgtcact cgatccgcct ccgagcccgg cccgggtggg
      721 gatcttcttg gtcagagcaa tctcgacgcc gaacttggcg ccagtcgctt cgagcacggc
      781 cttcgccgcg cgcaccgcga ggttctcgtc ggcgggaccg agatcggggc cgtccacctc
      841 cagcttcacg ccgcggtcgg tccgccgcac ttcgatctgg tccgccagct cgagcagcag
      901 aaacgcggtc tcgatctggt ggtagcccgc atgctcccgc gccaggatcc gcaggaagag
      961 gttgaccttg gcgtgggccg caacgcgcag ctggtccggg ttcacgcggt gtccgccctg
     1021 gctccgcgca gatctcccag gtgcagccgg gcgcgagaga gggaatagag cccgagcgcc
     1081 gtgaccggaa tgaaggtggt gaggtgatag gtgagggcgt acgcgagcgc ccggctcggg
     1141 tccacgtgat agagctccag cgtcaccttg gtcgcggttt caaacacccc cgcatatccg
     1201 ggagacgatg ggatcgccac gccgaagccg aggattccct gcaagaggaa cgagccgacc
     1261 gccggcaccg aaaggccgaa cgccttgaag cagagcgcga acgaggcggc gttgacgagc
     1321 cactgcacca gtgaccacca gagcacggcg atgaaccgtc cgggctttcg gagtacggcc
     1381 aggccttcga tgagcccatg caacaggtcg ttcacccggt gggcgacccg ttcggggagc
     1441 gccgcgtggg tcaggcggtc ggctagcctg agccagggag cgggatgcag ggcgacgacg
     1501 aacgcgacga ccaggaaccc cgagaacacg atggctgcgc cccgggcgac gtgggccagc
     1561 gagacgccct cgaccagggt gttcgcgggg aatgacggag cggcgatggc gaccgccatc
     1621 aacgcgagca tgaagagacc gtcgaagagc cgctcgacgc cgatggtggc gagcgcggtg
     1681 atgaatcgca cgggggcctg gcgcttcacc acgtacgcgc gcgcgaattc gccggcccgc
     1741 gcgggcagga ggttgttcgc catgaatccg atcgccaccg catgccagag cgcggaccag
     1801 gcgatgggcg cgtcgtcatc cccccgcagc atcacccgcc agcggatcat ccggagcggg
     1861 aaggtgcatg tggcgatgac cacgactaat agaagaaggg ggacgttgac ggtgcggagt
     1921 gcggcaccga agtcggccca gttcactccc ctgacggccc accacaggca cgcgatggtg
     1981 atcgcgagcc ccaggagcca ggcccaggag gctggtcgct taggaggggt cgagggcgag
     2041 gtcgacaaga tcgcagactt catcgacgag cgcgcgcaac tcctcgcctg agacattgcg
     2101 cgcgtgggcc ctcagctccg ccgcccgacg gcgcgccgcg tccccgcgat acaacagggt
     2161 gcggatgtcc acgacctgct gctcgacggg tgccggtgcg ggtgccggcg ccggagccgg
     2221 cttcgacgcg gggggcggtg gaggagcggg ggctgcccgg accgcaacgg gcggcggagc
     2281 gacggcggtc ccgcccgcga tcagcacatc gagtgacgcc tctccgagct cgccagcggt
     2341 cacgcgctcg tacatcgccc acgtacccac caggtcgggc gtctcttcga tcgagtcttc
     2401 caccggcggc gcgggggccg gcgtcggtgt cggagctggg gcaggggccg acggttccgg
     2461 ggccagagat tcgatcggaa ccacgggcac gggggacgcc ggactcgcgc ccaggccctt
     2521 gatcttcgcg gtcacgtcgg cgagctcggt cgcgagcgac ccctcctctt cagtcgacga
     2581 ccgggtgagg atgtctccgg cgcgccggag ttccgcggaa aacctggcgg cctcccgcac
     2641 cgcggcacca tccctgacgg catcgcgtgc cgcctcggcg aactcgctga ccccggcggc
     2701 gagcggacca ccaccttcat tcatcagggc gcggaaggtc ccggcaaggg tgtgagcgcg
     2761 cagctcgcgc tgcgtcgcgg acggcgcccg ttcgagagag tccgcggctt gccggaggtg
     2821 ctcgccatgg ctcaccagct cgagcttgcc gagcgtggct gggcgggccg agaaggtgcc
     2881 gcgcttgacg acgtgcgggc cgtcgtcggc gtagtagagg gacgcgatcg gtacgacgcc
     2941 gggctccgct tccttgctcc cgagaaacgc gacgagcttg gccgcgaagg ccttgaaggt
     3001 ggcgccttcg ggatcgggcc ggccctgctc ggccacttcc cgcgccgctc cggcgatggc
     3061 actcgcggcg gtctggaaca gctcgtccac cccgggcgat ggtgcgcctt cgtttcgatc
     3121 cagctccgcg atcgcgcgct cgatgccttc gagcacgtcg ggcagcgggg ggaggtcgtt
     3181 cagtgcggcg aggccgcgca ggggctgcag ggccttggtg accaggtgga gggggtcccg
     3241 tccgccagga tttgcgcgca gcgcctgcga cgcgcgatcg agcgcgctcg cgatggccgc
     3301 gccctcgcgg gccacgaacg cccgcgcacc ggcatcgagg gagggcgcgt ccatcttggg
     3361 tacggcccct gacgcacggg gcgttccggc cagacgctcc agctcgccgg agagggcttc
     3421 ggctttcacc gagtccgcat cggtccagtt caagacggac cggatgagaa tcttcagatc
     3481 gtcgacggcg cggatggtga tctgctgggt ctcggcgtcc caattgcggc gtccctcacg
     3541 gaccgcgcgc accagggcct cgagtcccgc ggcggcccgg gcgatcaggc ccttgttggc
     3601 catgagtgcg ctgccgcgga gggcccggga gagcttcagc atctcgtcca cgggaggtgg
     3661 agtggtaccg ccggccagcg ccccatccag gcgttccaga tactcccccg cctcgagggt
     3721 gaagaaatcc gtcatccggt gggggccggt cgggggcgtc gatgagctca tcggggattc
     3781 aagctagggg cgcgcgaatg gccggtcaaa caagccgtcg gttgtcagcc atcagcacgc
     3841 ttgctgacgg ctgatggctg atggctgatc gctccatcca ccaactctct catcctcctc
     3901 acggccccct ccagccccca gaacaccgcg ttgctcacga tcgagtgtcc gatattgagc
     3961 tcctcgatct ccggaatggc ggcgacgggg cggacgttga ggtaggtgag tccgtggccg
     4021 gcgtgaacgg cgagtccggt ctcgcgggca tgccgcgatg ccttctgaag ctggacgagc
     4081 gcccgcgcgt tcttccgcca ggtgtgggca taccgcccgg tatgaagctc gacggccggc
     4141 acgcccaggc ccgccgcgcg gtcgatggtg cggggatcgg gatcaatgaa gagggagacg
     4201 cggatgtcgg cgtcctgcag ccggctgatg gcacccgcca gacggcggtc gcgcggcttg
     4261 agcgcgagtc ccccctcggt ggtgatttcc tggcggcgct ccggcaccag cgtggcctga
     4321 tgggggcgga gccgggcggc gatcctgacg atctcgggac tcgccgcgag ctcgagattg
     4381 agaaccacgc cggggcgggc ggcgagggcc ttcacgtccg cgtcctgaat gtggcggcgg
     4441 tcttcgcgca ggtggacggt gatgccgtcg gcgccgccgg cgagcgcgtc ggccgcggcg
     4501 cggacgggat cgggctcgtc cgttttccgc gcttcgcgga tggtcgcgac gtgatcaatg
     4561 ttgacgtaga ggcgcatgag ttcttacctt
//
LOCUS       DASUBP010000179         7178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1040715, whole genome shotgun sequence.
ACCESSION   DASUBP010000179 DASUBP010000000
VERSION     DASUBP010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7178
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1040715"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="VFU75_06690"
     CDS             <1..931
                     /locus_tag="VFU75_06690"
                     /inference="COORDINATES: protein motif:HMM:NF013662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HEU5466559.1"
                     /translation="IVEAITIAGYAVGAEQGYVYIRGEYPLAIRRIQGAIDQARQAGL
                     LGPSVMGKGFAFDVEIRKGAGAYICGEETAIFNSIEGYRGEPRNKPPFPVAVGLFRKP
                     TLVNNVETLANVPAIVLGGGAAFAKIGTADSTGPKLFCLSGAVRNPGVYEVPFGTTLH
                     QLIALAGGPPEGRTLQAILLGGAAGMFVPGDTPDIPLTFEGARAAGVTLGSGVVMAFD
                     DQVDMKDTLLRIAAFFRDESCGQCVPCRVGTVRQEEALHRLVAKTPLGSAAQELVLLE
                     EIGAGMKDASICGLGQTAYSAVESAVKRLKVFQ"
     gene            928..1881
                     /locus_tag="VFU75_06695"
     CDS             928..1881
                     /locus_tag="VFU75_06695"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU5466560.1"
                     /translation="MTATHTGPLVPLAGPRRDVELTIDGKAVTVPEGTTILDACARLD
                     QTLPTLCYLETLHPVNACRVCVVEVEGSRVLVPACSRAVEAGMQVRTNTARVKHSRKM
                     VLEFLASSVDLSTTPHVSDWLAEYGARPDRYGPPDHPGTEGDRDSRSPGHHEPPRNGV
                     AATVAQPMKVDNDLYVRDYAKCILCYKCVEACGVDYQNTFAIAVAGRGFDARISTEFA
                     VELPESACVYCGNCIAVCPTGALMFKTERDHRDAGTWDESRQHVTETICPYCGVGCTL
                     ELRVQDNEIVRVTSPLDHSITRGNLCIKGRFGWRYVQNRRD"
     gene            1874..2734
                     /gene="fdhD"
                     /locus_tag="VFU75_06700"
     CDS             1874..2734
                     /gene="fdhD"
                     /locus_tag="VFU75_06700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HEU5466561.1"
                     /translation="MTDRVPPALSPEHPLFGLGYTEPVREREYTRLEGGHRSEKAPVA
                     EEVPIAFIYNGRPHVVMMGTPADLEDFAVGFSLTEEIVQRASDLSRIEVVRHAQGIEL
                     QLTIPEDAGKRLESRGRQLVGQTGCGLCGVETVAEALRPLRQLPVGRPPVPRHALWRA
                     EEVLPELQEWNRETGALHAAAWVSDAGAPEVVREDVGRHNALDKVIGALARSGRRADE
                     GFLLVTSRASYEMVQKATVAGARMLAAVSRPTGLAVRLAEASGLTLVALLRGRTANVY
                     AHPEGLTGPD"
     gene            complement(2718..3260)
                     /locus_tag="VFU75_06705"
     CDS             complement(2718..3260)
                     /locus_tag="VFU75_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466562.1"
                     /translation="MRRIIAFMLLLSAPAVAQSPYGGGGMGNGTGRSGMGDTPRPTTI
                     MDPLPSPEDLAGPAIPEFVIDRFELDTAEGKAYRVAYDSFMVATKALRDTASAVRQHI
                     DQLWQSGDRESARAYFPMLRRLGDRLAKEDDHFDDRVKKIFTKPHFKDYKDWRSDQRK
                     QAEEDRKERMKQLTGTNPGR"
     gene            3342..3926
                     /locus_tag="VFU75_06710"
     CDS             3342..3926
                     /locus_tag="VFU75_06710"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5466563.1"
                     /translation="MTARARGVMPDPSPEDERAIVARARTGDREAFGSLVDIYQNAVR
                     RVTRAVLHDAEEADDAAQDAFLSALTKLAQYDPGRPFRPWLMRIAANMAIDRKRRRQV
                     RRGQPLAPEAHSHEPSPQLHAERAALREKLEEAMTHLPERYRVALVLYDVEGYSHPEI
                     AEILGIPVGTVRSAVFHARRTLRPLLEGWREGGS"
     gene            3923..4285
                     /locus_tag="VFU75_06715"
     CDS             3923..4285
                     /locus_tag="VFU75_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466564.1"
                     /translation="MILDFDSHDAPTDPKVKAALQWLLPVTGREEFRARVISRAEAMQ
                     GRPAGTLDRWARPALAAAATIALAAWVAGGLLEPASTDALSLDGAMVSSATGSPPSAF
                     IASPRPPDADAIAGLVTP"
     gene            4282..4710
                     /locus_tag="VFU75_06720"
     CDS             4282..4710
                     /locus_tag="VFU75_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466565.1"
                     /translation="MTGLPVDSGRGSRGWVLAAFLVTFLAGAAVDHGVIIWRHRHDPI
                     MFRRPIGRPDMVSGLTRELDLSPVQQDSARAIFARHRCALRDIWQAAHPRFDSLRNRV
                     DSELSAVLNPDQRAKFLRLAGRHDHRPPPPMATDSGCAGS"
     gene            4783..5688
                     /locus_tag="VFU75_06725"
     CDS             4783..5688
                     /locus_tag="VFU75_06725"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU5466566.1"
                     /translation="MDVFVTGAGGYVGGAAARALRRAGHRVFGLTRTDVKARALESQE
                     IIPVLGDLADPTTYTSAAARCAVVIHAAFDDKSRGIPKDRAAVEGLLEATRRGGGAQP
                     RTFIYTSGVWVHGDTRGALVDETCPLDPLSLVSWRPPHEALVLEAPGVRGIVLRPGCV
                     YGGMGGMPGQWFASAAEGLPPEIVGDGANRWALVHIEDLGEAYRLAAESGLAREVFDL
                     VERSAETVREMAVAAARAAGLSGEIRSRPLIDARKSLGPYADALAADQRIDGGKAERL
                     LGWRPRHHGFVAEAEAFYGAWKAAR"
     gene            5685..6653
                     /locus_tag="VFU75_06730"
     CDS             5685..6653
                     /locus_tag="VFU75_06730"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HEU5466567.1"
                     /translation="MKAVRIETHGGPEVLRVVELPTPKPGPGQVLVGIEAVGLNFIDT
                     YQRSGLYQLPLPAVLGQEGAGRVVSLGADVKTLKVGDRVAFAHVMGAYADSVVIAADR
                     LVPVPDGVTSQVAAAVLLQGMTVQYLVTDTFGLKPGHTCLIHAAAGGVGLLLIQVAKL
                     KGATVIGTVSTPEKAQLARGAGADHVINYVEEDFEPAVRQLTGGRGVQVVYDSVGKTT
                     FAKSLNVLAPRGMLVTFGQSSGAVPPFDPLLLSQRGSLFLTRPVLGHYIATRDELLAR
                     ADETLGWVAGGRLKVRIDSEYPLAAVADAHRRLESRKSAGKILLIP"
     gene            6700..6966
                     /locus_tag="VFU75_06735"
     CDS             6700..6966
                     /locus_tag="VFU75_06735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466568.1"
                     /translation="MDTNRERRRSQNDRRVFERRRKFDRRSGGHSRPSIGTTVESARE
                     HIRNAIQIFKRIEVGEAPAEWSADADAAIHRLTRALADLDAGRI"
     gene            complement(6975..>7178)
                     /locus_tag="VFU75_06740"
     CDS             complement(6975..>7178)
                     /locus_tag="VFU75_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004398583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HEU5466569.1"
                     /translation="AAGGKVEISSSANQDSPLMDGKTPILGLDVWEHAYYLKYQNRRP
                     DYIGAWWNVVNWPEVARRFAAAK"
BASE COUNT         1100 a         2371 c         2512 g         1195 t
ORIGIN      
        1 gatcgtcgag gccatcacga tcgccgggta tgcggtgggc gcggagcagg gctacgtcta
       61 catccggggc gagtacccac tcgccatccg ccgcatacaa ggggcgatcg atcaggcccg
      121 tcaggcgggg ctgctcggtc cgtcggtcat ggggaagggc ttcgcgttcg acgtcgagat
      181 caggaagggc gcgggcgctt atatctgtgg ggaagagacc gcgatcttca actccatcga
      241 ggggtaccgc ggcgagccgc gcaacaagcc gcccttcccg gtggccgtcg ggctgtttcg
      301 caaacccaca ctcgtcaaca acgtcgagac cctggccaat gtccccgcga tcgtcctcgg
      361 tggcggggcg gcgttcgcaa agatcgggac agcggactcg accggcccca aactgttctg
      421 cttatcgggg gcagtccgga accccggtgt ctacgaggtg cccttcggca ccacgttgca
      481 ccagctcatc gccctcgcgg gtggaccgcc ggaaggccgg actctgcagg cgattctcct
      541 cggcggcgcc gccgggatgt tcgttccggg cgacactccc gacatccctc tgaccttcga
      601 aggagcgcgc gcggccggcg tcaccctcgg ctcaggggtc gtgatggcgt tcgatgatca
      661 ggtcgacatg aaggacacgc tcctgcgcat cgccgcgttc ttccgtgacg agtcatgcgg
      721 gcagtgcgtc ccctgccgcg tggggaccgt gcgccaggaa gaggcgctgc accgcctcgt
      781 ggcgaagacg ccgctcggct ccgcggcgca ggagctcgtc ttgctggagg agatcggggc
      841 ggggatgaag gacgcatcga tctgtgggct gggacagacc gcctacagcg ccgttgaatc
      901 ggctgtgaaa cgtctgaagg tcttccaatg accgccacgc acacaggccc cctcgttccc
      961 ctggccggac cacgccggga cgtcgagctg accatcgacg ggaaagcggt gacggtgccg
     1021 gaaggcacca ccatcctcga tgcctgcgcc cggctcgacc agaccctgcc gacgctctgc
     1081 tacctcgaga ccctgcatcc ggtgaacgcg tgccgcgtct gcgtggtgga ggtcgagggc
     1141 tcgcgcgttc tggtcccggc gtgctcgcgt gcggtcgaag ccggcatgca ggtgcggacc
     1201 aacacggcgc gggtgaagca cagccgcaag atggtcctgg agttcctcgc ctcgtccgtg
     1261 gatctgtcga ccactccgca cgtgtcggac tggctcgccg aatacggtgc ccggcccgac
     1321 cggtatggtc ctcccgacca ccccggcaca gagggcgacc gtgactcccg ttctcccggg
     1381 catcacgagc ccccccggaa cggcgtcgcc gccaccgtcg cacagccgat gaaggtcgac
     1441 aacgacctgt acgtccggga ctacgcgaag tgcatcctct gctacaagtg cgtggaagcg
     1501 tgcggcgtgg actaccagaa cacgttcgcg atcgcggtcg cgggtcgggg cttcgacgcg
     1561 cgcatctcga cggagttcgc cgtggagctg ccggaatccg cgtgcgtcta ctgcggcaac
     1621 tgcatcgccg tgtgtcccac gggtgccctg atgttcaaga ccgagcgcga tcaccgggac
     1681 gcgggcacct gggatgaaag ccggcagcac gtgaccgaga cgatctgccc ctactgcggc
     1741 gtcggctgca ctctggagct ccgcgtccag gacaacgaga tcgtccgggt cacgtctccc
     1801 ctcgaccatt cgatcactcg gggcaacctc tgcatcaagg gccgcttcgg ctggcggtac
     1861 gtacagaacc gccgtgactg accgggtccc gcccgccctt tccccggagc acccgctttt
     1921 cggtctcggc tacactgagc cggtgcgaga gcgggagtac accaggctcg agggcgggca
     1981 ccggagtgag aaagcaccgg tggcggaaga agtccccatc gctttcatct ataacggccg
     2041 ccctcatgtg gtcatgatgg ggactcccgc ggacctcgaa gacttcgccg tcgggttctc
     2101 tctgaccgag gagatcgtcc agcgcgcgtc cgacctctca cggatcgagg tcgtccgtca
     2161 cgcgcagggg atcgagttgc agctcacgat ccccgaggat gcggggaagc gcctcgagtc
     2221 ccgcggacgg cagctggtgg gccagacggg gtgtgggctg tgcggcgtcg agacggtcgc
     2281 tgaagccctg cggccactgc gccagctgcc ggtgggccgg ccgcccgtcc cgcggcacgc
     2341 gctgtggcgc gccgaggagg tcctgcctga gctgcaggag tggaaccggg agaccggtgc
     2401 gctgcacgcg gcggcgtggg tgagcgacgc gggagccccc gaagtggtgc gagaggatgt
     2461 cgggcggcac aacgcgctcg acaaggtgat tggtgcgctg gcgcgcagtg ggcgccgtgc
     2521 cgacgaggga tttctgctgg tgacgagccg cgcaagctac gagatggtcc agaaggcgac
     2581 cgtggcgggg gctcgaatgt tggcggcggt gtcccggccg acgggactgg cggttcggct
     2641 ggcggaggcg agtggcctga cgctggtggc actgctccgc ggccggacgg ccaacgtgta
     2701 cgcccacccc gagggactta ccggcccgga ttagttcccg tcagctgctt catgcgttcc
     2761 ttccggtcct cctccgcctg cttccgctga tcactgcgcc aatccttgta atccttgaag
     2821 tgcggcttcg tgaagatctt cttgacccgg tcgtcgaagt ggtcgtcttc cttggccagc
     2881 cggtcgccga gacgccgtaa catcgggaag taggcgcgcg cggactcgcg gtctcccgat
     2941 tgccagagtt ggtcgatgtg ctgacgcacg gcgctcgccg tgtcgcgcaa agccttggtc
     3001 gccaccatga acgaatcgta ggccacccgg taggccttcc cctcggcggt gtccagctcg
     3061 aaccggtcga tcacgaattc cggaatggcg ggtcccgcca gatcctcagg cgagggaagg
     3121 ggatccatga tggtcgtcgg acggggcgtg tctcccattc ccgaccgccc ggtcccattc
     3181 cccatgcccc ccccgccgta tggagactgg gcgactgccg gggccgatag caacagcatg
     3241 aacgcgatga tccgccgcat ggaatcctcc tcccgctaat ctacaccgct cgccccgggg
     3301 agggtggtca accccgggac gcgggtctcc gtaggtgtgg tgtgaccgcc agggcccgag
     3361 gggtgatgcc ggatccgagt ccggaagatg aaagggcgat cgttgcgcgg gctcggactg
     3421 gagaccgcga ggcgttcggt tcgctcgtgg acatctatca gaacgccgtg cgccgcgtga
     3481 ccagggcggt cctgcacgac gccgaggaag cggacgatgc cgcgcaggac gcgttcttgt
     3541 cggccctgac caagctcgcg caatacgacc caggacggcc ctttcgaccc tggctcatgc
     3601 gcatcgcggc gaacatggcg atcgaccgga aacgccgccg gcaagtgcgc cggggccagc
     3661 ccctggcgcc ggaagcgcac tcgcacgaac cgtctccgca gctgcacgcg gaacgggccg
     3721 cactccggga gaagctcgag gaagcgatga cgcatttgcc ggagcggtac cgggtggcgc
     3781 tggtactgta tgacgtcgaa ggctattcgc atccggagat cgctgagatt ctcggcatcc
     3841 cggtcggcac ggtgcggtcc gcggtatttc acgcccggcg gacgctacgt cccctgttgg
     3901 aggggtggag ggaggggggt tcatgattct cgacttcgat tcacacgacg caccgacgga
     3961 tcccaaggtc aaagccgcgc tccagtggct gctgccggtg accgggcggg aggagttccg
     4021 ggcccgggtg atatcccggg ccgaggccat gcaggggcgt cccgccggca ccctggatcg
     4081 ctgggcgcgt ccagcgctcg cggccgccgc cacgattgcg ctggccgcgt gggtggcggg
     4141 tggtctgctc gaacccgcat ccacggacgc cctatcgctc gacggtgcca tggtgagctc
     4201 ggccaccggg agtccgccct cggcgttcat cgcgtccccc cggccacctg acgccgatgc
     4261 catcgccggg ctggtgacgc catgaccgga ctcccggtgg atagcggccg cggttcacgc
     4321 ggctgggtgc tggcggcctt cctcgtcacc ttcctggcgg gcgcggcagt cgatcacggc
     4381 gtcatcatct ggaggcaccg ccacgatccg atcatgttcc ggcgccccat cggccgtccg
     4441 gacatggtgt ccggactcac cagggagctc gatctctcgc cggtccaaca ggactcggcc
     4501 cgggcgattt tcgcgcgcca tcggtgcgcc ctgcgcgata tctggcaggc ggcgcacccg
     4561 cggtttgatt cgttgcggaa tcgcgtcgac tccgagctct cggcggtcct caatcccgat
     4621 cagcgcgcca agtttctgcg gctcgctgga cgtcacgatc accggccgcc cccgcccatg
     4681 gccaccgact cggggtgtgc cggctcctga cgggtacacc ctcccgaacc cggggtcctt
     4741 cggcgacgtt gtagtcggga atggaccact tcgggggctg acatggatgt gttcgtcacc
     4801 ggtgccggtg gatacgtagg gggcgcggcc gcccgcgccc tccggcgcgc cggccatcga
     4861 gtcttcgggc tgacgcggac tgacgtcaag gcgcgcgccc tcgagtcaca ggaaatcatt
     4921 ccggtcctcg gcgatctcgc cgatcccacc acgtacacga gcgccgcggc gcgttgcgcc
     4981 gtggtcatcc acgccgcctt cgacgacaag tcccgcggca tcccgaaaga ccgcgcggcc
     5041 gtggaaggcc tgctcgaggc cactcgtcgg ggagggggcg cgcagccccg gacgttcatc
     5101 tatacgagtg gtgtgtgggt ccacggggac acgcgtggcg cattggtgga cgagacctgt
     5161 ccactcgatc ccctctcgct ggtgtcctgg cgtccacccc atgaggcact cgtcctcgaa
     5221 gccccgggag tgcgtggcat cgtcctgcgg ccggggtgtg tctacggcgg catgggaggg
     5281 atgcccgggc aatggttcgc ctcggcggcg gagggtttgc cgcccgagat cgtgggcgac
     5341 ggggcgaacc ggtgggccct cgtgcacatc gaggaccttg gggaagccta tcgcctggcc
     5401 gcggaaagtg gactggcgcg ggaggtgttc gacctggttg agcgctcggc cgaaacggtc
     5461 cgcgagatgg cggtggccgc tgcccgcgcg gcggggttga gcggcgagat ccgctcgcgg
     5521 ccgctcatcg atgcgcggaa gtcgttggga ccctacgcgg acgcgctcgc ggcggatcag
     5581 cgcatcgacg gcggcaaggc tgagcggctc ctggggtggc gcccccgcca tcacggtttc
     5641 gtggccgaag cggaggcttt ttatggagcc tggaaggccg cccgatgaaa gcggtcagaa
     5701 tcgagacgca tggcggacct gaggtgctgc gggtggtcga gctgccgacg ccaaaaccgg
     5761 gtccagggca ggtactggtg ggcatcgaag cggtgggcct gaacttcatc gatacctatc
     5821 agcggagtgg gttgtatcag ctcccgctcc ctgccgtgct ggggcaggag ggggccgggc
     5881 gcgtggtcag cctcggcgcc gatgtgaaaa ccctgaaggt gggggaccgg gtggcattcg
     5941 ctcacgtgat gggggcgtat gccgacagtg tcgtcattgc ggcggaccgc ctggtgccgg
     6001 tgccagacgg ggtgaccagc caggtcgccg cggccgtgtt gctccagggg atgaccgtgc
     6061 aatacctggt caccgacacc ttcggtctga agccgggcca cacctgcctg atccatgccg
     6121 cggccggagg cgtggggctc ctcctgatcc aggtcgccaa gctcaagggc gcgaccgtga
     6181 tcggcacggt atcgacgcct gagaaggctc agctggcccg gggcgccggc gccgaccacg
     6241 tgatcaatta cgtcgaggag gatttcgagc cggcggtgag gcagctgacg ggcgggcgcg
     6301 gggtgcaggt cgtctacgat tcagttggga agacgacgtt cgccaagagc ctgaacgttt
     6361 tggcgcctcg ggggatgctg gtgaccttcg gtcagtcgag tggcgccgtc cccccatttg
     6421 atcccttgct gctcagccag cgcggttcgc tttttctgac ccgcccagtt ctcggtcatt
     6481 acatcgcgac ccgggacgag ctgctggcga gggcggacga aacgctcggc tgggtcgccg
     6541 gcggacgcct gaaggtgcgg attgattccg agtatccact cgcggccgtc gcggatgcgc
     6601 atcggaggct cgagtcgcgg aagtcggcgg ggaaaatact cctgatcccc tgagccgatc
     6661 ccctggcgcg aatggcgttc gttcctacat tggggggccg tggatacgaa tcgggaacgc
     6721 cgccgcagcc agaatgaccg ccgcgtcttc gaacgccggc ggaagttcga ccgccgctcg
     6781 ggtggtcatt cgcgtccttc catcgggacg acggtagagt ccgcccgcga gcacatccgc
     6841 aacgcgattc agattttcaa gcggattgaa gtcggggagg cgccggcgga gtggtccgcc
     6901 gatgccgacg ccgccatcca ccgcctcacc cgcgccctcg ccgacctgga cgccggccgg
     6961 atctagccgc cggattactt cgcagcggcg aaccgccgcg cgacctcagg ccaattcacg
     7021 acgttccacc acgccccgat gtaatcgggc cggcggttct ggtatttcag gtagtaggcg
     7081 tgctcccaca cgtcgaggcc caggatgggc gtcttgccgt ccatcagcgg gctgtcctga
     7141 ttggcgctgc tcgagatctc gaccttcccg ccggccgc
//
LOCUS       DASUBP010000180         2293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_986218, whole genome shotgun sequence.
ACCESSION   DASUBP010000180 DASUBP010000000
VERSION     DASUBP010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2293
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986218"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..418
                     /locus_tag="VFU75_06745"
     CDS             101..418
                     /locus_tag="VFU75_06745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466570.1"
                     /translation="MRTTFPRLRMDDERDRLAPGPDLDHEIDRRVFRHEDPVEPEPYS
                     SGYEASLMLEYALSRSGWMMDVANKGPYWVKLERGGECVMASGETVELALCRAALKAV
                     EAA"
     gene            566..1495
                     /locus_tag="VFU75_06750"
     CDS             566..1495
                     /locus_tag="VFU75_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5466571.1"
                     /translation="MTESQSLPATVLVAATLERVPVTAALEEAGFTVAEAESGVGALG
                     RARVVRPDAIILSHALPDMSGIEACRLLHADPLVGSNIPVVILTSENPSPAERVAGLS
                     AGVWDFFRAPRDTRELALKLQTYVEARRNTAAALTDSGIDPANGLHARPALTRRIREL
                     SGLMTRMHGALSCIVFDTDVPDPGAAAMVSQVVRVSDVVGILSPTQFAVLAPATSASG
                     AGSLARRVATILRNGPLSDGRGVQALLIGYDAVDNLTYAPINPPDLITRAIAAVRTGK
                     PEPANHWLRRYEPLVEGPRNTPVNAMFAFDRKG"
     gene            1495..2031
                     /locus_tag="VFU75_06755"
     CDS             1495..2031
                     /locus_tag="VFU75_06755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466572.1"
                     /translation="MSPKRRFLSMVGLLAGSAILGSCDRTPVSPTVPAAQPVPDVRSD
                     WFGWGSNLLLKCSPQPFDSASQTIGPDGGTLTVGSNTLSIPPGALSVPTLITGVVNPD
                     TVNAVRFAPQGLVFQQPAQLVMSYANCGVFGSLIPKRIAYTTDDLLILQIIPSLDDVL
                     NQKVTGQVSHFSQYAIAW"
     gene            2036..>2293
                     /locus_tag="VFU75_06760"
     CDS             2036..>2293
                     /locus_tag="VFU75_06760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466573.1"
                     /translation="MRTIGPSHLLSEARREEESGAITEAMNRYGAAIAAASDDQELAV
                     LAEALRRLAVVLHQRDETGRAWELCRQSYQVACELGSDVLAA"
BASE COUNT          360 a          776 c          754 g          403 t
ORIGIN      
        1 tggcgctcta tgaagcgaag cggagaggtg ccaatcaggt gtccgcggtg gcgagccccg
       61 gcgatccccc tctggggctc gccgcactcg gagccgcctg atgcgcacca cctttccgcg
      121 gcttcgcatg gacgacgaac gtgaccgcct ggcccccggt cctgatctcg atcacgaaat
      181 cgaccggcgg gtcttccggc acgaagaccc tgtcgagccg gagccataca gctcgggcta
      241 cgaagcgagt ctcatgctcg aatacgccct gtcccggtca ggctggatga tggacgtggc
      301 gaacaaggga ccgtactggg tcaaactgga gcgcggcggc gagtgcgtga tggcatccgg
      361 cgagactgtc gagctggcgc tctgtcgcgc ggccctgaag gccgtcgaag ctgcataggg
      421 cagcaggtaa cgaggggagt gaagcaccat tatgaagcct tccggcccgg cgcattcggt
      481 ggggcaggtc gaacggtccc gtgattccgg gagcttcgac ctggaccgac cgggtccgtt
      541 tgttgcattg aggggacgta catccatgac cgagtctcag tcccttcccg ccacggtgct
      601 ggtcgccgcc acgctcgagc gtgtgccggt gaccgcagcg ctggaggagg cgggctttac
      661 ggtggcggag gccgagtccg gcgtgggtgc gctgggccgc gccagggtcg tccggcccga
      721 cgcgatcatc ctctcgcacg ccctcccgga catgtccggc atcgaagcct gccgcctcct
      781 gcatgcagac ccgctggtgg gtagcaacat tcccgtagtc atcttgacga gtgagaaccc
      841 gagtcccgcc gagcgggtgg ccggtctctc ggccggcgtg tgggacttct ttcgcgctcc
      901 acgcgacacg cgcgagctgg cgctcaagct ccagacttat gtcgaggcgc gccggaatac
      961 ggctgccgcc ctgaccgaca gcgggatcga tcccgccaac ggactccacg cgcgccccgc
     1021 gctcacccgg cgcatccggg agttgagcgg actcatgacc cggatgcatg gcgcgctctc
     1081 gtgcatcgta ttcgacaccg acgtccccga ccccggcgcc gcggccatgg tttcgcaggt
     1141 cgtccgcgtt tcggacgtcg tgggcattct gagccccacg cagttcgcgg tcctggcccc
     1201 cgccaccagc gcgagcggtg ccgggtctct ggcccgccgg gtcgccacga tcctgcgcaa
     1261 tggcccgttg agtgacggcc gcggcgttca ggcgttgctg atcggatatg acgccgtgga
     1321 caatctgacg tacgcgccca tcaatccgcc cgacttgatc acgcgtgcca tcgccgcggt
     1381 tcggaccgga aaaccggagc ccgccaatca ctggctccgt cgctacgaac cgctggtcga
     1441 aggtccgcgg aatacacccg tcaatgcgat gttcgcgttc gaccggaagg gatgatgagc
     1501 ccgaaacgtc gtttcctgtc gatggtgggt ctcctggcag gttcggccat cctcggctcc
     1561 tgcgaccgga cgcccgtctc gccgactgtg cccgcggcgc aacccgtgcc cgatgtgcgg
     1621 tccgactggt tcggctgggg ctccaacctg ttgctcaagt gctcgcccca gcccttcgat
     1681 tcggcttcgc aaacgatcgg cccggatggc ggtactctga ccgtcggctc gaacacgctg
     1741 agcataccgc ccggcgcact cagcgtgccg acgctgatca cgggcgtcgt gaatccggac
     1801 accgtgaatg ccgtccggtt tgccccgcag gggctcgtct tccagcagcc cgcccagctc
     1861 gtgatgagct acgccaattg cggggtcttc ggatccttga tcccgaagcg gatcgcgtac
     1921 accaccgatg atctgctcat tctccagatc atcccgtctc ttgatgacgt cctcaaccag
     1981 aaggtgacgg ggcaggtttc ccatttttcg cagtacgcta ttgcctggta ggccgatgag
     2041 aaccattggc ccatcgcacc tcctgtcgga ggcgcgccgc gaagaggagt ccggagccat
     2101 cacggaggcg atgaaccgct acggcgcggc gatcgcggcc gcatcggatg atcaggagct
     2161 ggcggttctc gccgaggcgc tgcgccggct cgccgtggtg ctccatcagc gggatgagac
     2221 cggccgggcc tgggaattgt gccggcagag ctaccaggtc gcctgcgagc tgggaagcga
     2281 cgtcctcgcc gcc
//
LOCUS       DASUBP010000181         2876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_427506, whole genome shotgun sequence.
ACCESSION   DASUBP010000181 DASUBP010000000
VERSION     DASUBP010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427506"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VFU75_06765"
     CDS             complement(<1..750)
                     /locus_tag="VFU75_06765"
                     /inference="COORDINATES: protein motif:HMM:NF017745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HEU5466574.1"
                     /translation="MAVGSLDGLTKSYFDLRWHLDPVGASQSGIAAHDGRLGRFGKGT
                     LTPHLAAMKSLTLALEEVATDGVDEEIDRTALLNDLRVTQNRFEKERPQTKNPEFWLS
                     HLLNGLYLLLARRDRPDAHRARALTERLEEVPAFLEDARATLADPVRVYCETGRQMTA
                     GGLALVREAGVTISRLGAQDGLSSERVMAALDGARAALESFDKDLDRWTDSGSDEFAL
                     GEDAFNFRLHYEHVLRDTAPELWRYGHRLKEE"
     gene            complement(741..1973)
                     /locus_tag="VFU75_06770"
     CDS             complement(741..1973)
                     /locus_tag="VFU75_06770"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LeuA family protein"
                     /protein_id="HEU5466575.1"
                     /translation="MDERDLIYDWNAQGKPFDWAKAKFDLNDETLRDGLQSPSVRDPD
                     LETKRRLLHLMADLGIAAADIGLPGAGPRVVEQVRTLAREIRDHKLPIFPNCAARTVI
                     ADIEPIARIVQEIGIPIEAATFIGSSPIRQYAEDWTVDRILKSTEEAVTFATKQGLPV
                     MYVTEDTTRARPETLKQLYGAAINCGATRICLADTVGHATPAGVKALIKFVKEEIVKE
                     KRVAVDWHGHRDRGMGLANCLAAIEAGADRIHATALGVGERVGNAEMDLLIVNLKLLG
                     AYGGDMSKLAEYCRTVADAVGIPIPINYPVMGTDAFRTGTGVHAAAIIKAKKKGHDWL
                     ADRVYSSVPAAELGLEQRIDISPVSGLSNVKYWLSTHGFDPDNEAACKILFDAAKRTD
                     RVLSEDECRRLLAQVVWQ"
     gene            2026..>2876
                     /locus_tag="VFU75_06775"
     CDS             2026..>2876
                     /locus_tag="VFU75_06775"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5466576.1"
                     /translation="MPPDTIAQLLEHPRIADARAAIESSDSETLTLQSAVSGIASPTG
                     LEGKRAAWLVDRFRDAGLSNIRVDDAGNVHGWLGERTRGPAVVLAAHLDTVFGADVDV
                     TVRKGQDGRRLEGPGISDNARGLAALVVLARVLSRGSLPFARPVLFAGTVGEEGSGDL
                     RGVRHLFGSKEFAAHAMIAVDGAGVERIIHRGVGSVRMRARFRGPGGHSWAAFGVANA
                     ASAVGRAASALAEIPIPSDPPSSLSVVRLGGGTGLNSIPHEAWLDLDIRSESAESLQK
                     LEGLALAA"
BASE COUNT          433 a          955 c          977 g          511 t
ORIGIN      
        1 ttcctctttg agacggtgcc cgtaccgcca caactcgggc gcggtatccc gcaggacgtg
       61 ctcatagtgc aggcggaagt tgaaggcgtc ctcgccgagc gcgaactcgt cggacccgga
      121 gtccgtccag cgatcgaggt ccttgtcgaa agactcgagc gccgctcgag ccccgtccag
      181 cgccgccatc acccgctcgc ttgagagacc gtcctgggcg ccgagacggc tgatggtcac
      241 acccgcctca cgcaccagcg ccagcccgcc ggcggtcatc tggcgccccg tctcgcagta
      301 gacgcgcacc ggatcggcga gcgtggcccg ggcgtcctcc agaaaggcgg gcacctcctc
      361 gagccgctca gtgagggccc gcgcccggtg ggcgtcgggg cggtcgcggc gagcgagcag
      421 gaggtagagc ccgttgagaa ggtgagagag ccagaactcg ggattcttgg tctgcgggcg
      481 ctccttctcg aaccggttct gggtgacccg gaggtcattc aggagggcgg tacggtcgat
      541 ctcttcgtcc actccatcgg tcgccacttc ttccagcgcc agggtgaggg acttcatcgc
      601 ggcaagatgg ggcgtcagcg tccccttgcc gaatcgtccc agccgtccgt catgcgcggc
      661 gatacccgac tggcttgcgc ccaccgggtc gaggtgccag cggagatcga agtaggactt
      721 ggtaagcccg tcgagcgagc ctactgccat acgacctgcg cgagcaggcg ccggcactcg
      781 tcttcagaca ggacccgatc ggtgcgtttc gcggcgtcga acagaatctt gcaggcggct
      841 tcgttgtcgg ggtcgaaccc gtgggtggaa agccagtact tcacgttgga gagcccggac
      901 accggcgaaa tatcgatgcg ctgctcgagt ccgagctcgg cggcgggcac ggagctgtac
      961 acccggtccg caagccagtc gtgtcctttc ttcttcgcct tgatgatcgc ggcggcgtgc
     1021 acgcccgtgc cggtgcggaa ggcgtcggtg cccatcaccg gataattgat cggaatggga
     1081 atgcccacgg cgtcggcgac ggtgcggcaa tactcggcga gcttgctcat gtcccctcca
     1141 tacgcaccga gcagcttcaa gttgacgatg agaaggtcca tctcggcatt gcccacccgc
     1201 tcgcccactc ccaacgcggt ggcatggatc cggtcggcac ccgcttcgat ggcggccaga
     1261 cagttggcga gacccatccc gcggtcgcgg tggccgtgcc agtcgaccgc tacacgcttc
     1321 tccttgacga tttcttcctt gacgaatttg atcagcgctt tcacgccggc cggcgtggca
     1381 tgtccgaccg tgtccgccag acagatccgc gtggccccgc agttgatggc ggcgccatag
     1441 agctgcttga gggtctccgg ccgtgcccgg gtggtgtcct ccgttacgta catgacgggg
     1501 agaccttgtt tcgtggcgaa cgtgaccgct tcttcggtcg acttgaggat ccggtccacc
     1561 gtccagtctt cggcgtactg ccggatgggg gacgatccga taaacgtggc ggcttcgatc
     1621 gggattccaa tctcctgaac gatccgggcg atcggctcga tgtcggctat cacggtgcgg
     1681 gcggcgcagt tgggaaagat cgggagcttg tggtcccgga tctccctggc cagggtccgg
     1741 acctgctcga ccacccgggg tcctgccccg ggcaggccga tgtcggccgc cgcgatgccc
     1801 aggtcggcca tgagatgaag cagacggcgt ttggtctcga ggtcggggtc ccggaccgac
     1861 ggggactgga gcccgtcgcg aagggtttcg tcgttcaggt cgaacttggc cttcgcccag
     1921 tcgaacggct tcccctgtgc attccagtcg tagatgaggt cgcgctcgtc catagactag
     1981 catcacccac tccctcgcca ccgtcaaggc agttagagtg gtgtcgtgcc gcccgacacg
     2041 atcgctcagc tgctcgagca tccccgcatc gcggacgccc gcgccgcgat tgaatcatcc
     2101 gattcagaga ccctgaccct ccagtcggcc gtctcaggga tcgcgtcccc gacgggactg
     2161 gaaggcaagc gcgcggcgtg gctcgtcgac cgcttccgcg acgcgggtct ctccaacatc
     2221 agggtcgatg acgccggcaa cgtccacggt tggctgggcg agcgcacccg cgggcccgcc
     2281 gtggtcctgg ccgcacacct cgacaccgta ttcggtgccg acgtggacgt cacggtccgg
     2341 aagggacagg acgggcgccg gctcgaggga cccgggattt ccgacaacgc ccggggtctc
     2401 gccgcgctgg tggtgctggc gcgggtcctc tcccggggtt ccctgccatt cgcccgacct
     2461 gttctgttcg ccggcacggt cggtgaggag gggtcggggg atctccgtgg cgtccgccat
     2521 ctattcggct ccaaggagtt cgcggcacat gcgatgatcg ccgtcgacgg cgcgggggtg
     2581 gagcgcatca tccaccgggg cgtcggatcc gtgcggatgc gggcacgatt tcggggaccc
     2641 ggggggcatt cctgggcggc gttcggcgtg gccaacgcgg ccagcgccgt cggacgggcc
     2701 gcgtccgccc tcgcggagat cccgattccc tcggaccctc cttcatcgct ctcggtggtg
     2761 cggctggggg gcggcacggg cttgaactcc atcccgcacg aagcctggct cgacctcgat
     2821 atccggagcg agagcgccga gtcgcttcag aagctcgagg gactcgcgct ggcggc
//
LOCUS       DASUBP010000182        16507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_236510, whole genome shotgun sequence.
ACCESSION   DASUBP010000182 DASUBP010000000
VERSION     DASUBP010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 16507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16507
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236510"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VFU75_06780"
     CDS             complement(<1..359)
                     /locus_tag="VFU75_06780"
                     /inference="COORDINATES: protein motif:HMM:NF025679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4382 domain-containing protein"
                     /protein_id="HEU5466577.1"
                     /translation="MRRIAPLAALTLVLALGCYTDDVIGPPGGKPSPVVRVLLTDDPF
                     PFDTVQSVSLYVVDIAAATQVDSLPDPQAFVTLASPHKTFNLLDLQQGTTAFLGQGTL
                     SAGQYRIVRMRIRTDSS"
     gene            456..1013
                     /locus_tag="VFU75_06785"
     CDS             456..1013
                     /locus_tag="VFU75_06785"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU5466578.1"
                     /translation="MGIQQVETTDGDLVRRVLAGDAHAYAGLVARHRDRLMRYARHVL
                     GNREDAEETVQDAFVRAYRSLARCDGPENYERWLFGILVNRCRTRYGQTARRSAVVVH
                     DDVAIAEAEAPGDADEATWGDAVRWALDQLEPLYREAFLLKHVEDLSYEEMAHLTRTG
                     VSALKMRVSRACERLRVMLEEVERV"
     gene            1006..1452
                     /locus_tag="VFU75_06790"
     CDS             1006..1452
                     /locus_tag="VFU75_06790"
                     /inference="COORDINATES: protein motif:HMM:NF027875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoamylase early set domain-containing protein"
                     /protein_id="HEU5466579.1"
                     /translation="MSESIERVVRELKRPVAVSPDLDAKVMAHVLADVRTPVPVRRLW
                     LWPALAVAAGLALLLFLPRGKDVPFTLVAPQASSVSVVGDFNDWKPTPLVKNRAGAWE
                     ARVRLAPGQYNYAFVVDGSRWVADPAAPRNAVDDFGSPNSVVTVAL"
     gene            1449..2006
                     /locus_tag="VFU75_06795"
     CDS             1449..2006
                     /locus_tag="VFU75_06795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466580.1"
                     /translation="MIKALLIALLVPAIAAGQAPAVQAIIDSAARAGLPTRPLVEKAL
                     EGSAKHAAPDAIAAAVRALAGALADARSALGTATTDELNAGAVALRLGARPEDLSRLR
                     AARSGGNLVVPLGVLSDLVARGVPVDTATRVVVALAANGDDEYLAFRQRVDRDIDLGA
                     APIGSVIGALNSSGRSALQSPGKKP"
     gene            2003..3091
                     /locus_tag="VFU75_06800"
     CDS             2003..3091
                     /locus_tag="VFU75_06800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466581.1"
                     /translation="MSCGASCLGGLAIFCLVGSSAGAQVSGSLDLGASDIRYDLFQPS
                     TALAISPTVAFDQGWTSVSARGTLLRFQSGHRDLHGSLVGTTFTPALGHFRLEVGADV
                     GASKYLSFPTFAHWFGAADLHYLRPTFGAWVGGTGGTTSFAGNAGTVEVGLWGTVPWA
                     TLTVTTSRASIGDTSYADIDASARVNQGRVEFIGRLGDRLGGKGGGHGVYGEASLTFA
                     LNATANLVVGGGRYATDPTRGTIAGRYATLGFRLATPAPRRGDPYREVLERYLPVPSA
                     DSPVTASAEVDAARVLRVRAEGAMKIEVMGDFTDWEPVLVTDAGILLTPGPHLLEVRI
                     DGGGWLVPLGATTVKDEFGGEAGLIVVP"
     gene            3186..4196
                     /locus_tag="VFU75_06805"
     CDS             3186..4196
                     /locus_tag="VFU75_06805"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HEU5466582.1"
                     /translation="MATPESWSSRLARYKGPNHLRSGLQLFTGVVLFAGTWALMYFSL
                     SVSYWLTLLLAAPAAFFLMRLFIIQHDCGHASFFRSAWLSDTVGSILGVLTLTPYHYW
                     KKTHAIHHATSGNLEHRGFGDIDTLTVEEYLARPRWERFKYRVYRHPAVLFGVGAVLH
                     FFVRHRLPTIVPKTWRRERRSIFWTDVGLAVFILVMGTLVGYREFALVQVPVMVLSCV
                     MGVWLFYVQHQFDPTYWEREDRWSYDSAALHGSSYYRLPKILQWATGNIGIHHVHHLH
                     PRIPNYRLDLVLRNHPELAAVPPLTLWGSLRCVRLTLWDEQARRLVSFKMVRERLSSP
                     RA"
     gene            complement(4504..6993)
                     /locus_tag="VFU75_06810"
     CDS             complement(4504..6993)
                     /locus_tag="VFU75_06810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HEU5466583.1"
                     /translation="MNPPAAQFVQMMDTVLLDLRYAIRSLRRSPGFTALAVLTLGLGI
                     GAATAGFAILHQVLLDPLPGVRDPGRLGMVVPAVRNGASFQPEALDADQRAEVLQSAP
                     AVAAMAGIQIADVDVAVPEEPPQRLMVNFVSGEYFALLGASPAQGRPLGPDDDLPDGG
                     TRVAVISDRLWQEGFGGRPDVVGQRLIVNGLSITVVGVAQPGFKGTERFEVPALWLPG
                     NAYFDLNHYRAGFHRPATYPYTESVMRLRAGASWDQATNQLESAVRTLAETDTADFDP
                     KVSANVFPGLGLSALGRDAIRRQVLLIIGIAGLVLLVTCANVANLLLFRRLQRQGDLV
                     VRLVLGAGRARLVRFFLVESGLLGLASGAVGAGVALLLRALFGELRLLNFLKMGPVTL
                     DIPVLAFAIVAGVIASLIAGLVPGVFGVRTDLNAHLKASGPTQAGGGRGLRVGLAVLQ
                     VAISLALVSGAYLVARTLRSYAEVPLGFDPAGVTMFEVEPKMLGYGEAQSHAYLRSLA
                     QRVGGLPGVTRVALTSSAPMEFSMMDGVLPEASPGTPIEVWDIPVSGAYFATVGMPLL
                     KGEVFGTADDWRDSSMKMGTAIVSLSLARQLFGDRDPVGELLKLRGGHGRSLRIIGVV
                     GDVRSGRLSDAPRPAVYEPMGQGFDSPWMMLAVQGGAASATIIKEVQGAGRALDPSLP
                     VESSGTLSDAVAAAVSSQTLLFKLVGSLSLFTLMLTAVGVYAIVAYGVTTRTREFGLR
                     MALGADTPKLVRTALNPALVIVSGGVVAGVLGALYLTKFIAASLYGVSRFDPLAFITA
                     ALILALAVLLASWLPARRAAKIDPMVALRYE"
     gene            complement(7160..9631)
                     /locus_tag="VFU75_06815"
     CDS             complement(7160..9631)
                     /locus_tag="VFU75_06815"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HEU5466584.1"
                     /translation="MIDTLLLDLRYAIRSLRRSPGFTALAVFTLGIGIGATTAGFSLL
                     NRLFLEPLPGVRDPHRLAFVTFMQPAGGAFGARGYSSPASPMPEEVPQVLKASPAVVG
                     LAGWQGTISMNVGVAGQLAVRVPGSFVTGNYLQLLGVDAQRGRILLPDDDPQPMGTRV
                     AVISDRLWSELYDRRSDIIGSTIIINNLPFTLVGVTARGFRGPDRISPCDLWVPGNTY
                     WDVRHFSTHGHPPIEIAYYRNVTRLRPGASFDQAQAQLQGAMQAVAVTDTAHFGGKVT
                     ATLVPGVGMERGGDAVRHELLLIMAVAGLVLLVACANIGNLLLFRRTQRRTDIVVRLS
                     LGAGRGRLVRLFLVESGLVGLAGGMTGILIAVGLKAAFGAMKILGFLDVGPVPLDIRV
                     IAFAVALGLTAALLSGAVPAVLGTSVDFNADLKASGPNQSGGAPRLRLALAAAQIAIS
                     LTLVAGMYLLAATLRNYAQIPLGFQPDGVTTFLTDPKEAGYDASRMRSYYDLMTQRIE
                     SLPGVTQVALVDLPPFTHVSFGLSLRAGAESLSANTIHVSSSYFATLGIPILRGTSFA
                     QADLWPDSLDPMKRVILSLSVTRSLFGDRDPIGQVIDVPDGRQPFRATVVGVAGDVRN
                     DFIGPVDRLVYQPVGDWPRLYSPSIMIKSRESSATLTRSVREVARGVDGSVTVESRGA
                     LPEAVAASINTEHLFFRVLGALSLLTMILTAVGVYGIVAYGVTTRTREFGIRSALGAM
                     PVNLVQVALRPALIINIVGTLGGVLGALYLTKFIAASLYGVSRFDPWAFITAALILAL
                     AVLLASWLPARRAAKIDPMVALRYE"
     gene            complement(9737..11092)
                     /locus_tag="VFU75_06820"
     CDS             complement(9737..11092)
                     /locus_tag="VFU75_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU5466585.1"
                     /translation="MTERSPSLARVLGFRDLVLIVVGTVIGSGIFLVPGNVIRASGGS
                     VGAALIVWLVGGVLSLLGALTYGELGARKPGAGGLYTYLRDAFGPVVAFLYGWTAFLV
                     IASGSVATLALAGSSYFSQLVPLSPGGAKGFALLVIALVTVINVRGTRGSANVQNWST
                     GIKVAALLVISAGLLVSGHGFSSGQAIWPAHWNGALISGMGIGMIGVLWAYEGWQYGT
                     FSAGETVDAQRVFPRAIALGTAALIGIYLFANVGYVAALGPAGVAGSDRVAAEAVGQV
                     ARPWAAKLVAAAILVSVFSAMNGLTITTPRMYYQMAQDGLFFKKLAEVHPRFGTPAVA
                     IVIGSVWAMVLTVWGTFESLLTYVVFVGWIFYALGALCVMVERRREEGGAGSFRVPGY
                     PWTPLAFVIAAAAIVVNTILTQPGRSALGIAGVLVGVPVYLLWKRALPKSERGTRSAE
                     P"
     gene            complement(11124..11888)
                     /locus_tag="VFU75_06825"
     CDS             complement(11124..11888)
                     /locus_tag="VFU75_06825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466586.1"
                     /translation="MSSRRLISALATALLVACNGGDQEAKPGPSTMVIGIDVSGSFRD
                     SKLYDDAVDFASQYIVAHLQGAGGLKVPTALFVGSIGGSRAGEPKAFHPINDFQGLTA
                     DQVAADIRNWFPPTDEWTDFNTFFGRVAGIVKERGLILAPLNVVVLSDGVPDLPPGTH
                     LAAGETPYQRIDLQPLEYLSRSVTVRLLYASPTVGDEWKRQVKRKRVRMWAEEGQVMT
                     GWHAQFKAGLPIDQQTDFLKWVKDNVDFRVRGGNIF"
     gene            complement(11885..13123)
                     /locus_tag="VFU75_06830"
     CDS             complement(11885..13123)
                     /locus_tag="VFU75_06830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466587.1"
                     /translation="MFALQQSLPEQWRETLQFVASGFTWILDWQRVVLGFSVTGDNLG
                     LTVLKAIVLLLPSALLVAAVWCTMASLYTLPFRSGRGGFLTALLLSWWDAGRMIWFYW
                     AGLLRFIVVLLGWLWGLVKLAGSLLAGFLKFIFTRPLALLDWATRQYFQPGVPWIAFL
                     FILFWSALEATIFTFTLKPMLIEVLADLTGTELSSLVVALILWPFLFILISGSFAAIQ
                     ALTEAIRAKKTSQIISMALVEAFVMFFEVMFLYRELIDAITPWIAQQTGFQLGFASTL
                     ALASFGWIGVRAMTWFLFGRFGTPALLGILGRQSIAGTQGATTQQPALQPDFWKAPIA
                     ALKAEAEWFRKEAREVFELMTLPVLQVLAAGLNFAVVVILGKPVFVLPFRNLEAVLAS
                     TPQLGSSGGGAAKHPTTGVV"
     gene            13391..14743
                     /locus_tag="VFU75_06835"
     CDS             13391..14743
                     /locus_tag="VFU75_06835"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5466588.1"
                     /translation="MSPSRHTIVTLIALFAPSFAATQQAPPAHDSRVAQIAEAGNAKN
                     IEHTIRTLVGFHTRNTISDTLSPTTGIGAARRWIHAQFDSISKACGGCLEVSYVSDIV
                     QPRPRVPRPTNVVDVVAVLKGQSDSGRYVVMTGHYDSRASDDQDDTVSAPGADDDGSG
                     TAAVIEAARILSKYKFNATIVFSALAGEEQGLLGGGILAQAAKARGWNVEGDLNNDIV
                     GGGRGLNGADDPTVVRIFSESPATNTSVQELARMRSVGGEEDSPSRQLARYIDRVDAG
                     YFPKFHVMLVYRQDRYGRGGDHIAFNREGFAGVRFTEPNENYNRQHQTPRVENGVFYG
                     DVPDSVDFGFTAHVAALNAASLASLAWAPAPPDSATVARFEGVSTHVHWKPVPGAAGY
                     RVYWRATTSPTWTNSRWVGDSTETVFSKMTIDDSYFGVASVSKTGQESLVAFPMPGRN
                     "
     gene            complement(14776..15999)
                     /locus_tag="VFU75_06840"
     CDS             complement(14776..15999)
                     /locus_tag="VFU75_06840"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HEU5466589.1"
                     /translation="MNGLTNQSAGKLGARLALPEAPLLLGTILLVHLPSLHGLLVSFG
                     KFYPVAAFALGLFLSLRYQRARLLFCLVVVALAAWGPLAAKLDGRQMIFDAAALLVPL
                     NLLVLAILPDRGFFTPAGWRKWAVILGELLAVAIIAQTGPAAGDAFFRASLLAPGIVR
                     FIGLPAPAILAFVLAFAMLAVLTIREPDGPGRGALWGLVACGIALTMEKPGVGATVAF
                     STAAVALSVAIIETSYHQVYHDALTGLPGRRALDEALLRLGDQYAVAMVDVDHFKKFN
                     DTFGHDVGDEVLRMVAARLSTVEGGGKAYRYGGEEFSILFPGITAAQATPALEAVREA
                     VADSEFTVRKRLRPRRKPTPLPVRERARHSAITVSIGVAEPGGRNKTPDQVLQAADRA
                     LYQAKEQGRNRVVGP"
     gene            16121..16300
                     /locus_tag="VFU75_06845"
     CDS             16121..16300
                     /locus_tag="VFU75_06845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466590.1"
                     /translation="MKSPGLAVFVMFFGLSLLDALWNPNWIAAGFWLACGLGFWALDR
                     GVWSRPMTRAGDRRS"
     gene            complement(16328..>16507)
                     /locus_tag="VFU75_06850"
     CDS             complement(16328..>16507)
                     /locus_tag="VFU75_06850"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5466591.1"
                     /translation="EMPLFKSTLMITPRKVVTHTVLHEIRHWAQIATMLRFEGVTDGF
                     HDFLFSPVMGGSGQS"
BASE COUNT         3030 a         5517 c         5409 g         2551 t
ORIGIN      
        1 ctcgacgagt cggtccggat ccgcatccgc acgatgcggt attgcccggc gctcagggtc
       61 ccttggccga ggaaggccgt ggtgccctgc tggagatcga gcaggttgaa ggtcttgtgg
      121 ggacttgcca aagtgacgaa agcctgcggg tccggcagcg aatccacctg ggtggcggcc
      181 gcgatgtcga ccacgtacag gctcacggac tgcacggtat cgaagggaaa ggggtcgtcg
      241 gtcaggagga cccggactac cgggctgggc ttgccgcctg gaggcccgat cacgtcatcg
      301 gtgtaacaac ccagagccag gaccaacgtc agggccgcga ggggagcaat tcgccgcatc
      361 acatgagtct ccgcgtgaag agaacacgtt tccagtcacc accggatacc cgacccgcag
      421 accggacgtc acaaagtgac gcgagtgggg gacccgtggg tatccagcag gtggagacta
      481 ccgatgggga cctggtacgc cgggtgctcg ccggcgacgc gcacgcgtat gccggcctgg
      541 tggctcgcca ccgtgaccgg ctgatgcgct atgcgcggca cgtgttgggg aaccgggaag
      601 atgccgagga aacggttcag gacgccttcg tccgggccta tcggtccctg gcgcgatgtg
      661 acggtccgga gaactacgaa cgctggctct ttggcatcct ggtgaaccgg tgtcgtacgc
      721 gatacggaca gacggcgcgg cgctccgccg tggtggtgca cgatgatgtg gcgatcgccg
      781 aggcggaagc gccgggcgac gcggatgagg cgacgtgggg agatgccgtg cggtgggcgc
      841 tggatcagct ggagccgctc tatcgcgaag cgttcctgct gaagcacgtc gaagacttga
      901 gctacgaaga gatggcgcat ttgacgagaa cgggagtatc ggccctgaag atgcgggtga
      961 gccgggcatg cgaacgactg cgcgtgatgc tcgaggaggt ggaacgtgtc tgagtcgatc
     1021 gaacgggtgg tgcgtgagct gaagaggccc gtggccgtct cgccggacct ggatgcgaag
     1081 gtcatggcgc acgtgctggc ggacgtccgg acgcccgtcc ccgtccggcg gctgtggctc
     1141 tggcccgcgc tggcggtcgc ggccgggctg gccctgctcc tgttcctgcc gcggggcaag
     1201 gacgtgccat tcaccctggt cgcgccgcag gcctcgagcg tctcggtagt cggtgacttc
     1261 aacgactgga aaccgacgcc cctggtcaag aacagggccg gcgcatggga ggcccgcgta
     1321 cgcctcgcgc ccggccagta caactatgcg tttgtcgtcg acggctcccg gtgggtcgcg
     1381 gatcccgcgg cgccgagaaa cgcggtcgac gatttcggat ctcccaactc ggtggtaacg
     1441 gtggccctat gatcaaagcc ttgctaatcg ccctgctggt gccggcgatc gccgccggtc
     1501 aggcacccgc cgttcaagcc atcattgatt ccgcggcgcg cgcgggcctt ccgacccgcc
     1561 ctctggtcga gaaggcgctc gagggctctg ccaagcacgc cgctcccgac gccattgccg
     1621 ccgcggttcg ggccctggcg ggggcgctgg ccgacgcccg gagcgcgctc ggcacggcga
     1681 cgactgacga gctcaacgcg ggcgcggtgg cactccggct cggcgcccgt ccggaagatc
     1741 tctcccggct ccgcgcggcc cgttccggcg gaaacctggt cgtgcccctg ggcgtcctct
     1801 ccgatctggt cgcacgaggc gttcccgtgg acacagccac cagagtggtc gtggcgctcg
     1861 ccgcgaatgg tgacgacgag tacctcgcat tccgccaacg cgtcgaccgc gacatcgatc
     1921 tgggtgcggc cccgatcggc tcggtcatcg gcgcactcaa ttcctcgggg cgctcggcgc
     1981 tccaatcccc cggcaagaag ccgtgagttg cggcgcctcc tgcctgggag gcctcgcgat
     2041 attctgcctc gtcggatcgt cggcaggcgc ccaggtctcg gggtctctcg acctgggcgc
     2101 ttcggacatc cggtacgacc tcttccaacc atcgaccgcg ctcgcgattt cgccgaccgt
     2161 cgccttcgac caggggtgga cgtcggtctc cgcgcgcggc accctcctcc gcttccaaag
     2221 cggccaccgc gacctgcacg gatccctggt gggaacgaca ttcactccgg cactcggcca
     2281 tttccggctc gaagtcggtg cggatgtcgg cgcgagcaag tatctctcgt tccccacctt
     2341 cgcccactgg ttcggcgccg ccgatctcca ctacctgcgg ccgaccttcg gagcctgggt
     2401 cggtggcacc ggcgggacca cctcgttcgc cggaaacgcc gggacggtgg aggtcgggct
     2461 gtgggggacg gtgccctggg ccactctcac cgtgacgacg tcacgcgcct cgatcggcga
     2521 cacgagctac gcggacatcg atgcgtcggc gcgcgtgaat caggggcggg tggagttcat
     2581 cggccggttg ggggaccggc tcggtggcaa gggagggggg cacggcgtgt acggtgaggc
     2641 gtcgctgacg tttgccctga atgcgacggc gaatctcgtg gtgggcggcg gccgttacgc
     2701 caccgatccg acgcgcggaa cgatcgccgg ccgttacgcg accctggggt tccgcctcgc
     2761 cactcccgcg ccgcgtcgtg gcgacccgta tcgcgaggtc ctcgagcgtt acctgcccgt
     2821 gccctccgcg gactctccgg taaccgcctc cgccgaggtg gacgcggcca gggtgctgcg
     2881 ggtacgcgcc gaaggggcga tgaagatcga ggtcatgggg gatttcacgg attgggagcc
     2941 ggtcctggtg accgatgcgg ggatcctcct gacaccggga ccgcacctgc tggaggtccg
     3001 gatcgacggg ggcggctggc tggtgcccct cggggcgacg accgtgaagg atgagtttgg
     3061 aggcgaggcg ggactgatcg tagtgccctg aaaccgaagt cggaatgcgg agtgcggaat
     3121 gcggaactgc aggccaattc gtgcttgccc tttgagatag tcgtacccca gaccatattg
     3181 gggaagtggc aactcccgaa tcctggtcct cccgcctcgc ccgctacaag gggccgaacc
     3241 acctccgcag cggcctgcag ctcttcacag gcgtcgtgct gttcgcgggg acttgggccc
     3301 tgatgtactt cagtctctcg gtgagctact ggctcaccct cttgctcgcc gctccagccg
     3361 cattcttcct gatgcggctt ttcatcattc agcatgactg cgggcacgcc tcgttcttcc
     3421 gctcggcctg gctgtcggac accgtcggct cgatcctggg cgtgctcact ctgacgccgt
     3481 accactactg gaagaaaacc cacgcgattc accacgcgac gtccggtaac ctcgaacacc
     3541 ggggcttcgg tgatatcgac acgctgacgg tggaggagta cctggcccgc ccccgctggg
     3601 agcggttcaa gtaccgcgtc taccggcatc cagcggtgtt attcggggtc ggggccgtgc
     3661 tgcacttctt cgtccgccat cggcttccca cgatcgtgcc gaagacgtgg cgtcgggaac
     3721 gccggagcat cttctggacg gacgtgggcc tcgcggtgtt catcctggtg atgggcacgc
     3781 tggtgggata ccgcgaattc gcgctggtac aagtccccgt gatggtactg tcgtgcgtga
     3841 tgggcgtctg gctgttctac gtgcagcacc agttcgaccc gacctactgg gagcgggagg
     3901 accgctggtc gtacgactcc gcggccctgc acggcagctc gtattaccgg ctgccgaaga
     3961 tcctgcagtg ggccaccggc aacatcggga ttcatcacgt ccatcacctg catccgcgca
     4021 ttcccaacta ccgtctggac ctagtgctgc gcaaccatcc ggagctggcg gcggtgcccc
     4081 ccctcacgct gtgggggagc ctgcggtgcg tccggctgac gctgtgggac gaacaagcac
     4141 gccggctggt gtccttcaag atggtgcgcg agcgccttag ctccccgcgc gcctgagggc
     4201 cgctttgacc cgggctacca gccgcggtcc atcgatcggt ttccgcacgt agtcgtctgc
     4261 cccgcgatcc atgatttgca cctcggtatc gctcgactcg gaacccgtca gcacgaggat
     4321 taccggtcag ccgtcccggt cgttcccgcg ccctgatcgg gcgctctcgg ccgggcgcgg
     4381 tctgaagacc tccactgccc gggtgactcg gccgtgcaag cccgtcaggg cgcgctcgcg
     4441 ccgagagcgg cctgtgcttt agcggctctc agcccgcggg aatgacgtgg ggaggggtat
     4501 cccctactcg tacctcagcg ccaccatcgg gtcgatcttc gcggcgcgcc gggcggggag
     4561 ccagctggcg agaaggaccg ccagggcaag gatcagcgcg gcggtgataa aggccaatgg
     4621 gtcgaaccgg ctgacgccgt agagcgacgc cgcgatgaac ttggtgagat agagcgcacc
     4681 gaggacgcct gccacgacac cccccgacac gatcacgagc gccggattga gggcggttcg
     4741 aaccagcttg ggggtatccg cgccgagtgc catgcgcaga ccaaactcgc gagtgcgggt
     4801 ggtgacaccg taggcgacga ttgcgtacac cccgaccgcg gtaagcatga gcgtgaacag
     4861 agagagcgaa ccgacaagct tgaacaacag cgtctgactg gagaccgccg cggccactgc
     4921 gtcactgagc gtgccactgg actcgaccgg cagggaggga tcgagcgcac gtcccgcccc
     4981 ctgcacctcc ttgatgatcg tcgcactcgc cgcaccgccc tgcacggcga gcatcatcca
     5041 gggcgaatca aacccctgtc ccatcggctc atacaccgcg ggccggggag cgtccgagag
     5101 ccttccggaa cgcacgtcgc ccaccacacc gatgatccgc agagatcgac cgtggccgcc
     5161 ccgcaatttc agcagctcac cgaccggatc gcgatcgccg aacagttgcc gggcgagtga
     5221 gaggctgacg atcgccgtcc ccatcttcat agatgagtcg cgccaatcgt ccgcagtccc
     5281 gaacacctcg cccttgagca acggcatgcc gacggtcgcg aagtaggctc ccgataccgg
     5341 aatgtcccag acctcaatgg gcgttccggg tgaggcctcc ggcagtacgc cgtccatcat
     5401 gctgaactcc atcggtgccg agctggttag ggccacccgc gtgacgccag gaagtccgcc
     5461 gacccgctgg gccagggatc gaaggtaggc gtgactttgt gcctcaccgt accccagcat
     5521 cttgggctcg acttcaaaca tggtcacccc ggcgggatcg aatccaagcg ggacctccgc
     5581 gtaactccgc agggtccggg caacgaggta cgcgccagaa acgagggcga gtgagatggc
     5641 gacctgtagc acggcgaggc cgacgcggag tccgcggcca ccccccgctt gtgtgggtcc
     5701 ggatgccttc aggtgggcgt tgagatcggt ccggaccccg aacacgccgg gcaccaagcc
     5761 cgcgatcaag gaggcgatca cgcctgcgac gatcgcgaat gccaggaccg ggatgtcgag
     5821 ggtgaccggc cccatcttca ggaagttcag gaggcgcaat tctccgaaga gcgcgcgcag
     5881 gagcagcgcc acacccgcac ccaccgcccc actcgccaat ccgagaagcc cgctctccac
     5941 cagaaagaag cgcaccagac gggcccgacc cgcaccgagt accaggcgga ccaccagatc
     6001 gccctgccgt tggaggcgtc gaaagaggag cagattggcg acgttcgcgc aggtgaccag
     6061 caacaccaga ccggcgatcc cgatgatgag gagaacctgg cgtcggatgg cgtcccggcc
     6121 cagcgccgac agcccgaggc ccgggaacac atttgcgctc accttcggat cgaaatcagc
     6181 agtatccgtc tccgccaggg tccgcaccgc gctctccagc tggttggtcg cctggtccca
     6241 cgaagccccc gcccggagac gcatcacact ctcggtgtac ggatacgtcg cggggcggtg
     6301 gaatcccgcc cggtagtggt tgagatcgaa gtacgcgttc ccaggcagcc agagcgccgg
     6361 cacctcgaac cgctccgtgc ccttgaagcc cggctgcgcc actccgacca ccgtgatcga
     6421 aaggccgttg acgatcaatc gctgacccac cacgtcgggg cggccgccga atccctcctg
     6481 ccacaaccgg tcgctgatca cggcgacacg ggtgcctccg tcggggaggt cgtcgtcggg
     6541 cccaaggggc ctgccctgcg caggtgacgc tcccaacagg gcgaaatact cgccgctcac
     6601 gaagttcacc atgaggcgtt gcggcggctc ctcgggaacg gcgacatcca cgtcggcgat
     6661 ctgtattccc gccatggcag ccaccgctgg tgcggactgc agaacctcgg cccgctggtc
     6721 ggcgtcgagc gcctccggtt ggaagctcgc gccatttcgc accgcgggca ccaccatgcc
     6781 tagccgcccg ggatctctga ccccagggag gggatcgagt aagacttggt ggaggatggc
     6841 gaaccctgcg gtcgcggcgc cgataccaag cccaagcgtg aggacggcga gggccgtgaa
     6901 cccgggactg cggcggagag agcgaatggc gtaacgaagg tcgagcagga cggtgtccat
     6961 catctgaaca aactgcgcgg cgggagggtt catgtccagc gctccatttt cgttcccgcg
     7021 ggctgatagc ccgctctcgg cgcgagcgcg ccctgaaggg cttgcacagc caactcgacc
     7081 gggcagtgga ggtctttaga ccgcgcccgg ccgagagcgc cctattaggg cgcgggaatg
     7141 acgtggggag gggtatcccc tactcgtacc tgagcgccac catcggatcg atcttcgcgg
     7201 cccgccttgc ggggagccag ctggcgagga ggaccgccag ggcaaggatc agcgcggcgg
     7261 tgataaaggc ccatgggtcg aaccggctga cgccgtagag cgacgccgcg atgaacttgg
     7321 tgagatagag ggcaccgaga acgccgccga gagttccgac gatgttgatg ataagcgccg
     7381 gtcgaagggc cacctggacc aggttcacgg gcatcgcgcc gagggcggat cggatgccga
     7441 attctcgagt gcgagtcgtg acgccgtacg ccacaatgcc gtagacgccg accgcagtga
     7501 ggatcatggt caacagagag agagccccca gcacccggaa gaacaggtgc tcggtgttga
     7561 tcgacgccgc aaccgcctcc gggagcgcgc ctcggctctc aaccgtaacc gacccatcaa
     7621 cccctctcgc aacctcccgg accgacctcg tcaatgtggc gctggactcc cgagacttga
     7681 tcatgatgga agggctgtag aggcggggcc agtctcccac cggttggtac accaaccgat
     7741 ccacgggtcc gataaagtca ttgcgcacgt ctcctgccac acccactaca gtggcccgga
     7801 aaggctgacg gccgtccggc acatcgatca cctggccgat cgggtcacga tcgccaaaga
     7861 gggaacgcgt cacactcagg ctgaggatca ccctcttcat gggatcgaga gaatcgggcc
     7921 agaggtccgc ctgggcgaag gaagtacctc ggagaatcgg aatccccaag gttgcgaaat
     7981 aactcgaaga cacatggatg gtgttcgccg aaagcgattc ggcaccggcg cgcagactga
     8041 gaccgaagct gacgtgcgtg aacgggggca agtccacgag cgcgacctga gtcacgcccg
     8101 gcaaggactc gatgcgctgg gtcatgaggt catagtagct ccgcatgcgg gaggcgtcgt
     8161 accccgcttc tttcgggtcg gtcaggaacg tggtcacacc atccggttgg aagcccagcg
     8221 ggatctgggc gtaattccgg agagtggcgg ccaacaggta catccccgcc acgagcgtca
     8281 gtgagatcgc gatttgggcg gcagccaggg cgaggcgaag gcggggagcg cctccggact
     8341 ggttcggtcc ggacgccttg agatcggcat tgaagtcgac ggaggtgccc agcactgcgg
     8401 gcactgcacc agacaggagg gccgcagtca agccgagtgc taccgcaaac gcgattacgc
     8461 ggatgtccag cggtacggga ccgacatcca gaaaccccag gatcttcatg gcgccgaaag
     8521 ctgccttcag gccgacagcg atcaggattc cggtcatacc tccagcgaga ccgacgagcc
     8581 cactctccac cagaaacagc ctgaccagcc ggccccggcc cgcgccgagg gacagccgga
     8641 ccacaatgtc ggtgcggcgc tgcgttcttc ggaacaacag gaggttgccg atgttggcgc
     8701 aggcgaccag cagcacgagt ccggcgacgg ccatgatcag cagtagctcg tgacgcaccg
     8761 cgtccccgcc gcgttccatc cccacacccg ggaccagtgt ggcggtcacc ttcccaccaa
     8821 agtgcgccgt atcggtaacc gccacggcct gcatggcccc ctggagctgc gcctgcgcct
     8881 ggtcgaatga cgctcccggc cggagtcggg tcacattgcg gtagtaggcg atctcaatcg
     8941 ggggatgtcc gtgagtcgaa aaatgccgga cgtcccagta cgtattgccg ggcacccaca
     9001 ggtcgcacgg ggagatccgg tccggccccc gaaagccccg ggcggtgacg ccaacgagcg
     9061 tgaatgggag gttgttgatg atgatcgtgg atccgatgat atccgaccgc cggtcgtaca
     9121 actcgctcca gaggcgatcg ctgatcacgg ccacgcgcgt gcccatcggc tgcgggtcgt
     9181 cgtccggaag gagaatcctg cctcgctgcg cgtcgacccc aagcaattgc aggtagttgc
     9241 ccgtcacaaa cgagcccggg acgcgaaccg ccagttgacc ggccacaccg acgttcatcg
     9301 atatcgtgcc ctgccagccg gccaggccaa ccactgcggg cgaggccttc agcacctgtg
     9361 gcacctcctc gggcattgga ctcgccggcg aggagtaacc ccgtgccccg aacgctccgc
     9421 ctgccggttg catgaacgtc acgaacgcga gtcgatgcgg gtcgcggacg ccgggaagag
     9481 gctcgagaaa cagacggttc agcaggctga agccagccgt tgtggcaccg atgccgattc
     9541 caagcgtgaa gacggcgagg gccgtgaacc cgggactgcg gcggagcgag cgaatggcgt
     9601 aacgaaggtc gagcaggagg gtgtcgatca tctgaacaaa ctgcgcattg gaagcttgcc
     9661 tgtccagtcg atgttgctgg gcgcaaacgc ggaacgcgga agtcggaatg cggaacccga
     9721 agaccccaag cccgacctac ggttccgcgc tccgcgttcc tcgttccgac ttcggcaatg
     9781 ctcgcttcca caagagatac accggcaccc ccaccagcac gccggctatt ccgagggccg
     9841 accttcccgg ctgcgtaagg atcgtgttca cgacgatcgc cgccgcggcg atcacgaacg
     9901 ccaggggcgt ccacggatat cccgggacgc gaaaggagcc ggcccctccc tcctccctcc
     9961 gcctttcgac catcacgcag agggccccga gggcataaaa gatccacccc acgaacacga
    10021 cgtacgtgag cagcgactcg aacgtccccc acaccgtcaa caccatggcc caaacacttc
    10081 cgatcacgat cgcgacggcc ggcgtgccga atctgggatg cacctccgcg agcttcttga
    10141 agaacaagcc atcttgcgcc atctggtagt acatccgtgg cgtcgtgatc gtgagtccgt
    10201 tcatggccga gaacaccgaa acaagaatcg cggccgcgac gagcttcgcc gcccacggcc
    10261 tcgccacctg ccccaccgcc tccgcggcga cccggtccga tccagccact cccgccggtc
    10321 ccagtgccgc gacgtagccg acgttggcga agagatagat cccgatcagt gccgccgtgc
    10381 cgagcgcgat ggcgcgcggg aacacccgtt gggcgtcgac ggtctccccc gccgagaacg
    10441 tgccgtactg ccagccttcg tatgcccaca gcacgccgat catcccgatc cccatcccgg
    10501 agatcagcgc gccgttccaa tgcgccggcc aaatggcctg gccactcgag aaaccgtgcc
    10561 ccgagaccag caaccccgcg ctgatcacaa gcagagcagc gaccttgatg cccgtgctcc
    10621 agttctggac gttcgccgac cctctcgtcc cacggacatt gatcaccgtg acgagggcga
    10681 tgaccagcag ggcgaagccc ttggcgcccc ctggggagag aggtacgagc tgggagaagt
    10741 acgagctgcc ggcgagggca agcgtcgcga cggaaccgga ggctatgacg agaaacgcgg
    10801 tccacccata cagaaacgcg acgaccggtc cgaaggcgtc ccggagatag gtgtagagtc
    10861 ctcccgctcc gggcttcctg gcaccgagct ctccgtacgt cagcgctccc aggagcgaaa
    10921 gcacgccccc gaccagccac acgatcagcg ccgcaccgac cgaaccaccg gacgcgcgaa
    10981 tcacgttccc cggcaccagg aaaatcccgg agccgattac ggtgccgacg acgatgagaa
    11041 cgaggtctct gaaaccgagg acgcgcgcga gggaggggga gcgttcggtc atgggccgaa
    11101 gctacccctc ctccacgcga ccgctagaag atgttgccgc cgcggacccg gaagtccacg
    11161 ttgtccttca cccacttgag aaagtcggtc tgttgatcga tcgggagacc cgctttgaac
    11221 tgcgcgtgcc acccggtcat gacttgccct tcctcggccc acatgcggac ccgtttccgc
    11281 ttcacctgcc gcttccactc atctcccacc gtcgggctcg catagagcaa ccgcacggtc
    11341 acgctccggg agagatactc gagcggctgc aggtcgatcc gctgatacgg ggtctctccc
    11401 gccgcgagat gcgtccccgg aggcaaatcg ggtacgccgt ccgagagcac caccacattc
    11461 agcggcgcca ggatcagccc ccgctccttc acgatccccg ccaccctgcc aaagaacgtg
    11521 ttgaaatcgg tccactcgtc cgtgggtggg aaccagttcc tgatgtccgc cgctacctga
    11581 tcggcggtga ggccctggaa atcattgatc ggatgaaagg ccttcggctc ccccgcccgc
    11641 gagcccccga tcgacccgac gaagagagcc gtcgggacct tgagcccccc tgccccctgg
    11701 aggtgcgcca cgatgtactg cgacgcgaaa tcgacggcgt cgtcatacag cttggagtcc
    11761 cggaacgagc cggagacgtc gatgccgatc accatcgtcg acggacccgg cttggcctcc
    11821 tggtcccctc cgttgcaggc gacgagcagc gccgtagcga gcgcggagat gagccggcgg
    11881 gagctcatac cacccccgtc gtcgggtgct tggccgcacc tcccccgctg gagccgagct
    11941 gaggggtcga cgcgagaacc gcctcgagat tccggaacgg cagcacgaac actggcttcc
    12001 cgagaatcac gacgacggca aagttgaggc ccgccgccag gacctgcagc accggcagcg
    12061 tcatcagctc gaacacttcc cgcgcttcct tccggaacca ctccgcctcg gccttcagcg
    12121 ccgcgatcgg ggccttccag aagtccggct ggagcgccgg ttgctgcgtc gtcgccccct
    12181 gggtgcccgc gatcgactgc cggccgagaa tgccgagcag cgccggcgtc ccgaagcggc
    12241 cgaagaggaa ccaggtcatg gcccgcacgc cgatccagcc gaacgaggcc agcgccagtg
    12301 tggaagcgaa ccccagctgg aaccccgtct gctgcgcgat ccagggggtg atcgcgtcga
    12361 tcagctcgcg atagaggaac atcacctcga aaaacatcac gaaggcctcg acgagcgcca
    12421 tcgaaatgat ctgcgacgtc ttcttcgccc ggatcgcctc ggttagggcc tgaatggccg
    12481 cgaacgaacc cgagatcagg atgaagagga agggccagag aatgagggcc acgacgaggc
    12541 tcgagagctc ggtgcccgta agatccgcca acacctcgat gagcatcggt ttgagggtga
    12601 acgtgaagat ggtcgcttcg agggcggacc agaacagaat gaacaagaac gcaatccagg
    12661 ggaccccggg ctggaaatac tgccgcgtgg cccagtcgag cagcgcgagc gggcgggtga
    12721 agatgaactt caggaagcct gccaggagcg agcccgcgag cttcacgaga ccccagagcc
    12781 agccgagcag gaccacgatg aagcgcagga gaccggccca gtagaaccag atcatccggc
    12841 ccgcatccca ccacgacagg agcagggcgg tgagaaatcc gccgcgcccc gaacggaagg
    12901 ggagcgtgta gagggacgcc atcgtgcacc acaccgcggc cacgaggagc gcggaaggca
    12961 gcaacagaac gatcgccttc agaacggtga ggccgaggtt atccccggtg acggagaatc
    13021 ccagcaccac gcgctgccaa tccaggatcc aggtgaaccc cgacgcgaca aactgcaacg
    13081 tctctcgcca ctgttccggg agagactgct ggagtgcgaa catacccacc tcgattcgaa
    13141 gtgagcacag ggtacccccg cgtcccagtg agttcaagcg atttacgtca caaacgcccg
    13201 accttccggt tcgtgagacg ggtcactggc gttccgccca cgcaacattt ctcggacatg
    13261 agcacgaact ttccactctt gcgggttcag gaatcggact gcagcatagg atcgccgtca
    13321 cacttcgccg tacgggaggg gcgctagagc ccctcccttt tttccatccg ccttccccgg
    13381 agccattgtc gtgagcccgt cacgtcacac gatcgtcacc ctcatcgcgc tgttcgcccc
    13441 gtccttcgcc gccactcagc aggcgccgcc cgcacacgac tcgcgcgttg cgcagatcgc
    13501 cgaggccggg aacgcgaaga acatcgagca caccatccgg accctggtcg gcttccacac
    13561 ccgcaatacg atctcggaca cgctgtcgcc gacgacgggg atcggcgccg cgcgacgctg
    13621 gattcacgcg cagttcgact cgatttccaa ggcctgcggc gggtgcctcg aggtttcgta
    13681 cgtgtccgac atcgtacagc cccgccctcg cgtgccgagg cccacgaacg tggtggacgt
    13741 ggtggcggtc ctgaagggac agagtgattc gggccgctac gtcgtgatga cggggcacta
    13801 cgactcgcgt gcgtcggacg accaggatga caccgtgagc gcccccggtg ccgacgacga
    13861 cggctcagga acggcggccg tgatcgaggc cgcgcggatc ctctccaagt acaagttcaa
    13921 cgcgacgatc gtgttctcgg cgctcgccgg tgaggaacag ggcctgctcg gcggcgggat
    13981 cctcgcccag gcggccaagg cgcgtgggtg gaacgtcgag ggggatctga acaacgatat
    14041 cgtcggtgga ggccggggct tgaacggcgc cgacgacccc accgtggttc ggatcttttc
    14101 ggagagcccg gcgaccaaca ccagcgtgca ggagctcgca cgcatgcgct ccgtgggcgg
    14161 cgaagaggac agcccgtccc ggcagctcgc ccgttacatc gaccgggtgg acgccgggta
    14221 cttcccgaaa ttccacgtga tgctggtgta ccggcaggac cggtacggac ggggtggcga
    14281 ccacatcgcc ttcaatcggg aaggcttcgc cggcgtgcgg ttcaccgagc cgaacgagaa
    14341 ctacaaccgc cagcaccaga cgccgcgcgt cgagaatggc gtgttctacg gcgacgtccc
    14401 ggactcggtc gatttcggct tcaccgccca tgtggcggcg ctgaacgcgg catccctcgc
    14461 ctcgctcgcg tgggccccgg cgccccccga ctcggcaacg gtggcacgct tcgagggggt
    14521 ctccacgcac gtccactgga aaccggtgcc cggcgccgcg ggctaccggg tgtactggcg
    14581 ggcgacgacg tcgccgacct ggaccaactc ccgctgggtg ggggatagca ccgaaaccgt
    14641 gttctcgaag atgacgatcg acgattcgta cttcggcgtc gcgtcggttt cgaagaccgg
    14701 gcaggaaagt ctcgtcgcct tcccgatgcc ggggagaaac tagacggcta gacggttaga
    14761 cggttagatg tcgacctagg gtccgacgac gcggtttctc ccctgctctt tggcctgata
    14821 gagcgcccgg tcagctgcct gcagcacctg atcgggcgtc ttgtttcggc cccccggctc
    14881 ggccacgccg atgctgacgg tgatcgccga gtggcgggcc cgctcgcgga ccggaagcgg
    14941 ggtcggcttc cttcgaggcc ggagccgctt ccggacggtg aattcagagt cggccacggc
    15001 ctcccggacg gcttcgagcg ccggggttgc ctgggccgcc gtgatccccg ggaagagaat
    15061 cgaaaactcc tcgccgccgt agcgatacgc cttgccgcca ccctcgacgg ttgaaagccg
    15121 cgccgccacc atccgcagca cttcatctcc cacgtcgtgc ccaaaggtgt cgttgaattt
    15181 cttgaagtgg tccacgtcca ccatggccac ggcgtactga tcgccgagcc ggaggagcgc
    15241 ctcatcgaga gcgcgacgtc cggggaggcc ggtcaacgcg tcgtgataga cctggtgata
    15301 ggaggtctcg atgatcgcca cggacagcgc gacggcggcc gtcgagaatg cgacggtggc
    15361 cccgaccccg ggcttctcca tcgtgagcgc gatcccgcac gccaccagtc cccagagtgc
    15421 gccccgcccc gggccgtcgg gctcacggat ggtgaggacg gcgagcatcg cgaacgccag
    15481 tacgaaggcg aggatggcgg gcgcgggcag accgatgaac cgcacgatgc cgggagccag
    15541 gaggctcgcc ctgaagaacg cgtcccccgc cgccggaccg gtctgcgcga tgatcgcgac
    15601 ggcgagcagt tctccgagaa tcaccgccca cttgcgccag ccggcgggcg tgaagaaccc
    15661 gcgatcgggg aggatggcca gcaccagcag gttgaggggg accagcaggg cggccgcgtc
    15721 gaagatcatc tgccggccgt cgagcttcgc cgccaacggt ccccacgccg caagggccac
    15781 gaccacgaga cagaacagca gccgggcgcg ctggtaacgg agcgagagga agaggccgag
    15841 ggcgaacgcg gcgacgggat agaacttgcc gaaggagacc agaagcccgt gcagcgacgg
    15901 caggtgcacc aggaggatgg taccgagaag gagcggcgct tcgggcagcg cgagcctcgc
    15961 tccgagtttc ccggcggatt ggttcgtgag cccgttcact gggctagaag cgtagcgacc
    16021 cactgccgtg ggtcaactgg caagcgtccg tcaccttctt ttcacggatc cttcacgcac
    16081 cgggcgggag cttcaaatcg gacactcgcc aggagtaccg atgaagtctc cagggctcgc
    16141 cgtgttcgtg atgttcttcg gtctcagtct gctggacgcg ctgtggaatc cgaactggat
    16201 cgcggctgga ttctggctcg cgtgcggcct tggcttctgg gcgctcgatc ggggagtgtg
    16261 gagccgtccg atgacccggg caggcgatcg ccgcagctag accgctcgag gcagcccccg
    16321 ctgacgctca cgactgcccg gaccccccca tcaccgggct gaacaagaag tcgtggaagc
    16381 catcggtgac cccctcgaac ctcagcatgg tggcgatctg agcccagtgc cggatctcgt
    16441 gcaacaccgt gtgcgtcaca accttccgtg gtgtgatcat caacgtcgac ttgaaaagcg
    16501 gcatctc
//
LOCUS       DASUBP010000183         3348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_277787, whole genome shotgun sequence.
ACCESSION   DASUBP010000183 DASUBP010000000
VERSION     DASUBP010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3348
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_277787"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VFU75_06855"
     CDS             complement(<1..680)
                     /locus_tag="VFU75_06855"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEU5466592.1"
                     /translation="MSALLADVPLSRSVEDYLKVIYHLSSQGGFATTSDIAAMLLVAP
                     PSVSGMVRRLSDSGLIEHVPYRGVQLTNQGRRAALRMIRRHRVLELYLTERLQYDWDG
                     VHDEAERLEHAVSDELIDRMAMALGNPQYDPHGAPIPTAAGEIEETVLVPLAEVEVGA
                     PYELRQVDTEDPARLRYLAEHGLVPGKGVTVTERQPFNGPVTLTLVPGGERVIGQELA
                     QLLLCKRA"
     gene            complement(811..1386)
                     /locus_tag="VFU75_06860"
     CDS             complement(811..1386)
                     /locus_tag="VFU75_06860"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HEU5466593.1"
                     /translation="MTSLDHVRRVVGSRPGVVDHDPSARPAAVAVILHEAADGISALF
                     IKRAEQVGDPWSGQIAFPGGRFEEGDSDLLSTAIREAREETGIDLGAAEQLGVLDDVN
                     PRSPHLPPIFVRPFVFALGHRPPHTNSPAEVQRSFWIPFNTLLDPATSDQITLTFRGV
                     PRTFTAYKVEGEVIWGMTERIVRLFLERITA"
     gene            complement(1383..1733)
                     /locus_tag="VFU75_06865"
     CDS             complement(1383..1733)
                     /locus_tag="VFU75_06865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466594.1"
                     /translation="MATPRKTEFPISNASAPWTLRSVGGSRPTLIIWGTTARESVLER
                     LARSGLAVLTPPEQATPADLMAALENVPAPVRVALGGTPSAEVSKILNAKEIPWRRVT
                     SDWDDVPSWFSETQ"
     gene            1778..2923
                     /locus_tag="VFU75_06870"
     CDS             1778..2923
                     /locus_tag="VFU75_06870"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5466595.1"
                     /translation="MIWLIPAGYLLALLLIVRFYWLRTPDLQDYQPDSDGPAVTVIIP
                     ARNEARNIEACVTSILATGYRRLEVIVVDDQSTDDTAARVSRLIALPGNGDRLRLVSG
                     ATKPEGWFGKQWALVQGFRAVKPGKGDLLVFADADTRHGAELIPRAVAVLAKEKVDLV
                     TVLPRQAMESFWERVIQPHVFFALGLGVGDFRRVNRTRKYWKGIANGQFILTPRKTYE
                     AVGTHEAVKDTVADDLALAQAYIKSDRDIFLVHAFPYMTTRMYTNLREIFEGWSKNLA
                     SGAPQMMPPIKLVRAAFPWVMWVPSLVWLVPPVILAITGALPWLVMILLSLAIWGFIS
                     ANEKVPVWYALLYPLGAGVMIAIMLRSAVRGRHIEWKGRVYGSRVVK"
BASE COUNT          578 a         1043 c         1106 g          621 t
ORIGIN      
        1 tcagcacgct tgcacaggag cagctgggcg agttcttgtc caatcacgcg ctcgccgccg
       61 ggcacgaggg tcagtgtcac cggtccattg aagggctggc gctcggtgac cgtcacgccc
      121 tttccgggaa cgagcccgtg ctcggcgaga tagcgcaggc gcgccgggtc ttccgtgtcg
      181 acctgccgca gctcgtacgg agcccccacc tccacctcgg cgagggggac cagcacggtc
      241 tcctcgatct cgccggcggc agtcgggatc ggtgcgccat gcgggtcgta ctgcgggttg
      301 cccagagcca tcgccatccg gtcgatcagc tcgtccgaca cggcgtgctc gagccgctcg
      361 gcctcgtcat gcacgccgtc ccagtcgtac tgcagccgtt cggtgaggta gagctcgaga
      421 acgcggtggc ggcggatcat tcggagcgcc gcacggcgcc cctggttggt gagctgcacg
      481 ccgcggtaag gaacgtgctc gatcaggccg gaatccgaga ggcggcggac cattccgctg
      541 accgacggag gtgccacgag gagcatcgcg gcgatgtccg acgtggtcgc gaacccgccc
      601 tggctcgaca ggtggtaaat gaccttgagg tagtcctcta ccgagcgcga cagaggaacg
      661 tccgccaaca aggcgctcat gtctgcctcc cgtatcgtgc tggccgcttg cgagttatcc
      721 agatcgggag acaatttagc ttcaactaat tcttaataca atccacagaa atattcggat
      781 tatatgaggc tatctcttgt tagtggataa ctacgccgtg atccgctcca ggaagagcct
      841 cacgatgcgc tcggtcatgc cccaaatgac ttcgccttcc actttgtagg cggtgaaggt
      901 tcggggcacc ccgcggaacg tcagggtgat ctggtcgctg gtggcagggt caaggagggt
      961 gttgaagggg atccagaacg agcgttgtac ctcggcgggg ctgttggtgt gggggggccg
     1021 gtgccccagg gcgaatacaa agggcctgac gaaaatgggg gggagatgcg gcgagcgcgg
     1081 attcacgtcg tcgaggaccc cgagctgttc cgccgcgccc agatcgatcc cggtctcctc
     1141 acgggcctcg cggatggcgg tggagagcag atcgctgtcc ccttcttcga accgcccgcc
     1201 ggggaatgcg atctggcccg accacgggtc ccccacctgc tccgccctct tgataaagag
     1261 cgcggagatc ccgtcggcgg cctcgtggag aatgacggcg accgcggcag ggcgtgccga
     1321 gggatcgtga tcgacgacgc cggggcggga gccgacgacg cggcggacgt ggtcgagtga
     1381 ggtcactggg tctccgagaa ccagctcggg acgtcgtccc aatcggaagt cactcgccgc
     1441 cacggaatct ctttcgcgtt caggattttc gagacctcgg cggacggagt tccgccgagc
     1501 gccaccctga ctggtgcggg tacgttctcc agcgcggcca tcaggtcggc aggggtcgcc
     1561 tgttcgggag gggtcagaac cgccaacccc gatcgagcca ggcgctcgag caccgactcc
     1621 cgtgccgtcg taccccagat gatcagcgtc gggcgagagc cgccgacgct cctgagcgtc
     1681 cagggagccg aggcattcga gatggggaat tcggtctttc gtggagtggc cacggtcggc
     1741 accttgtgag ctcgaaactt actaaggacc gtctcagttg atctggctca tcccggccgg
     1801 ctacctgctg gccctccttc taatcgtgcg cttctactgg ctccgcacgc cggacctcca
     1861 ggactaccag ccggattcgg acgggcccgc ggtcaccgtc atcattccgg cgcgcaacga
     1921 ggcccggaat atcgaggcgt gtgtcacgtc gattctggcg actgggtatc ggcgcctcga
     1981 agtgatcgtc gtcgacgacc agtcaaccga cgatacagca gcgcgggtct ctcggctcat
     2041 cgcgctcccc gggaacggtg accggttgcg gctcgtcagc ggcgccacga agcccgaggg
     2101 ctggttcggc aagcagtggg cgctggtcca ggggttccgt gccgtgaagc ctgggaaggg
     2161 agatctcttg gtcttcgccg atgcggacac ccggcacggc gccgagctca tcccccgtgc
     2221 cgtggcggtc ctcgcgaagg agaaggtgga tctcgtaacc gtgctgcctc gccaggcgat
     2281 ggagagtttt tgggagcggg tcattcagcc ccacgtgttc ttcgcgttgg ggctgggggt
     2341 cggcgacttc aggcgggtga accgcacccg aaagtactgg aaggggatag cgaacgggca
     2401 attcatcctg acgccgagga agacgtacga agcggtgggg acgcacgaag cggtgaagga
     2461 cacggtcgcc gatgacctgg ccctggccca ggcgtacatc aagtcggatc gcgacatctt
     2521 cctggtccac gccttccctt acatgacgac gcggatgtac acgaatctcc gcgagatttt
     2581 cgaagggtgg tcgaagaatc tcgcctccgg tgcgccgcag atgatgccgc cgatcaagct
     2641 ggttcgcgcc gcgttcccgt gggtcatgtg ggtgccgagc ctcgtgtggc tcgtgccgcc
     2701 cgtgattctt gcgatcacgg gagcgctgcc atggctcgtc atgattctgc tttcgctggc
     2761 catctggggc ttcatttccg ccaacgaaaa ggtccccgtg tggtacgcgc tcctctaccc
     2821 cctcggcgcc ggagtcatga tcgcgatcat gctccggtca gccgtgaggg gaaggcacat
     2881 tgagtggaag gggcgggtgt atggatcacg ggtggtgaaa tgaatttgcc cgcgtcggtt
     2941 cccgcgggct gagagcccgc tctccctctt ttttccttat tacgggcgag cgacgcccgt
     3001 catcctgagc gccgacggcg cgaaggacct gcttttcacc cgcagcacaa gcgggtcctt
     3061 cgctcgcgct tcgcgctcgc tcaggatgac gcgggcctcc ttttcttgag aaatgaaatg
     3121 gaagctcctg gggcgccgaa gaactgcgtc aggtaaccat tcgatgctct acacttgtag
     3181 ttcgaatcgc tggtccgaac ctgaccacgg tcacatcgtt gcaaccgtcg caccgcccgg
     3241 actatccaag tccgtgtgcc gaatacgact ccctggatcg aaagcctacg agagccgaca
     3301 ctggtcaccg ccgacgccga aatagctgac gcaactctgg ccgcgagc
//
LOCUS       DASUBP010000184         2341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_440820, whole genome shotgun sequence.
ACCESSION   DASUBP010000184 DASUBP010000000
VERSION     DASUBP010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440820"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..131)
                     /locus_tag="VFU75_06875"
     CDS             complement(<1..131)
                     /locus_tag="VFU75_06875"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HEU5466596.1"
                     /translation="MSDCLFCKIAAGEIPAAVVRKNDDFLAFRDINPQAPTHLLAIPV
                     "
     gene            complement(128..1279)
                     /gene="dnaJ"
                     /locus_tag="VFU75_06880"
     CDS             complement(128..1279)
                     /gene="dnaJ"
                     /locus_tag="VFU75_06880"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEU5466597.1"
                     /translation="MSDFYKILGVSMDATADDVKKAYRKLAMEHHPDRNNGDKKAEEK
                     FKEITEAYEVLRDPQQRAAYDRYGEAGVRGAGRGGGGRGPQMHHVDLSEALNIFMRDF
                     GGLGGFDAFFGGGQRSQRDRHRGQDIKVTLRLTLAEVATGTSKTVKIKGLDPCPECAG
                     TGAKKGTKVQTCRTCGGSGEVRRTAQSLFGPLVSIGACPSCEGEGTIVAEPCPECRGD
                     GRVRSEKTVQVDVPAGVADHHYLTLRGHGVPGPRNGPPGDLIAVLEIEDDPRFERHEE
                     DLVFDQALSFSQAALGADIEVPTPFGNTVLKIPHGTQTGTVFRLKGKGLPRVGNAGKG
                     DLHVRVHVWTPAKLSPEQERLFKEMAPHEGEPPSDVRKFWNQIKAAFGA"
     gene            complement(1276..2304)
                     /gene="hrcA"
                     /locus_tag="VFU75_06885"
     CDS             complement(1276..2304)
                     /gene="hrcA"
                     /locus_tag="VFU75_06885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEU5466598.1"
                     /translation="MPLTERERLVLEAVIETFVQTAEPAGSRTVSKVLGAFSPATIRN
                     TMSDLEEKGYLYHPHTSAGRIPTDLAYRAYVDLLMRQPPPAVTATEADAIRAELIGRS
                     SAVDVVLEKAAQVLAVLTKELGVAVGPTLEQAILERLDLTQVSSERLLLVLTLKSGVV
                     RTIFVEVPSHMASEVVGQVAVVLNERLAGLELREIRKTLSERLRDAAPGPGAGELLNI
                     FVQEADGLFDVNAAPASAVVLSSAQMLAGQPEFATTSRLQGLMEVTERRDVLREALTH
                     HREGLTITIGGEHQDPKLLPFTLVTARYEIGPLAGVIGVLGPTRMPYDKVVALVNHTS
                     RLVGELLE"
BASE COUNT          393 a          870 c          682 g          396 t
ORIGIN      
        1 accggaatcg cgaggaggtg cgtaggggct tggggattga tgtcccggaa ggccaggaaa
       61 tcgtcgttct tgcgcaccac cgcggcgggg atctctcccg ccgcgatctt gcagaacagg
      121 caatcactca tgcgccgaac gccgccttga tctgattcca gaacttccgc acatccgatg
      181 gcggctcccc ttcgtggggc gccatctcct tgaagagccg ttcctgttca ggcgacagct
      241 tggcgggagt ccagacgtgc acgcgcacgt gcaggtcacc tttccccgcg ttgcccaccc
      301 gcggcaatcc cttccccttc agccggaaca ccgtgcccgt ctgcgtccca tgcgggatct
      361 tgagcaccgt attcccgaag ggcgtcggca cctcgatgtc ggcgcccagc gccgcctggc
      421 tgaacgagag cgcctggtcg aagacgagat cctcttcgtg ccgctcgaac cgcggatcgt
      481 cttcgatctc gaggaccgcg atcagatcgc ccggcgggcc attccgcgga cccggcacgc
      541 cgtggccgcg cagcgtgagg taatgatggt ccgccacgcc cgcgggcaca tccacctgca
      601 ccgtcttttc ggagcgcacg cgcccatcac cccggcactc gggacagggc tccgcgacga
      661 tcgttccctc gccctcgcag ctcggacagg cgccgatcga caccagcggc ccgaacagcg
      721 actgcgcggt gcggcgcact tcccccgacc ccccgcacgt ccggcacgtc tgcaccttcg
      781 tgcccttctt cgcccccgtg cccgcgcact ccggacacgg atcgagtccc ttgatcttca
      841 ccgtcttcga cgtgcccgtg gccacctcgg cgagcgtcag ccgcaaggtg accttgatgt
      901 cctgaccgcg atggcgatcc cgctgcgagc gctggccgcc gccgaagaac gcgtcgaagc
      961 cgccgagccc gccgaaatcg cgcatgaaga tgttgagcgc ttcggagaga tcgacgtgat
     1021 gcatctgcgg accccgcccg cccccgcccc gccccgcgcc acgcacgccg gcttcaccgt
     1081 agcggtcata ggcggcacgc tgctggggat cgcggagcac ctcgtaggcc tccgtgatct
     1141 ccttgaactt ctcctccgcc ttcttgtcgc cgttgttgcg gtcgggatgg tgctccatcg
     1201 ccagcttgcg gtacgccttc ttcacgtcgt ccgccgtcgc atccatcgag acgccgagga
     1261 tcttgtagaa gtcgctcatt ccaggagctc tcccaccagg cgactcgtgt gattcaccaa
     1321 tgccaccacc ttgtcatagg gcatgcgcgt cgggcccagc acgccgatca ccccggccag
     1381 cgggccgatc tcgtaccgcg ccgtcaccag cgtaaagggc aggagcttgg gatcctggtg
     1441 ctcgccaccg atcgtgatcg tcaaaccctc gcgatggtgc gtcagcgcct cccgcaacac
     1501 gtcgcgacgc tcggtaacct ccatcagccc ctgcagccgg ctcgtcgtcg cgaactccgg
     1561 ctgccccgcc agcatctgcg cgctcgacaa caccaccgcg ctcgccggcg ccgcgttgac
     1621 gtcgaaaagc ccgtccgcct cctgcacgaa aatattgagc agctcacccg cgccgggacc
     1681 gggcgccgca tcccgcaggc gctccgacaa cgtcttccgg atctcccgaa gctcgagccc
     1741 cgccagacgc tcgttgagaa ccaccgccac ctgccccacc acttcggacg ccatgtgcga
     1801 cggcacttcg acgaagatcg tccgcaccac accgctcttc agcgtgagca ccaggagcag
     1861 acgctccgac gacacctgcg tcagatccag acgctccagg atcgcctgct cgagcgtcgg
     1921 ccccaccgcc acacccagct ccttggtcaa caccgccagc acctgggccg ccttctccaa
     1981 caccacgtca actgccgatg aacgcccaat caactcggcg cgaatcgcgt cggcttccgt
     2041 cgcggtgacc gcgggtggag gctgacgcat caggagatcg acgtaggcgc gataggccag
     2101 atcggtggga atgcggcccg cggaggtgtg ggggtggtag aggtacccct tctcctccag
     2161 gtcactcatc gtgttacgga tggtcgccgg ggaaaatgcg cccagaacct tggacaccgt
     2221 ccggctcccg gccggctccg cggtctggac aaacgtctcg atgacggctt cgagaacgag
     2281 ccgttcgcgc tcagtgaggg gcattatcgt acaagttctt tcagtggaac aatttacaaa
     2341 a
//
LOCUS       DASUBP010000185         2811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_154793, whole genome shotgun sequence.
ACCESSION   DASUBP010000185 DASUBP010000000
VERSION     DASUBP010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2811
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154793"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VFU75_06890"
     CDS             <1..462
                     /locus_tag="VFU75_06890"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5466599.1"
                     /translation="ITDRAPGFCRHQIVEMGYLKSAHQMTAESAAFNVQPRATGQVLI
                     GSSRELVGWDASVNHGLVAAMLSRAQRFMPALSKLQALRIWTGFRPATPDKLPLIGRW
                     ESTPGLWIAAGHEGLGITTSLGTAGLLADLIAGRTPAIAAIPFAPGRRMEP"
     gene            462..710
                     /locus_tag="VFU75_06895"
     CDS             462..710
                     /locus_tag="VFU75_06895"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU5466600.1"
                     /translation="MTRTLSIIIDGRLLEVPEGITVAAAMLNAGEDAFRDSVSGMPRG
                     AVCGMGICFECRVTIDGARGRRACLERVSPGMRVTTRE"
     gene            712..1974
                     /locus_tag="VFU75_06900"
     CDS             712..1974
                     /locus_tag="VFU75_06900"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HEU5466601.1"
                     /translation="MRDVSADLVVIGGGPAGIAAAARAGEAGKRVMVLDEAPHLGGQI
                     WRHLQRATLPAKARSWLDRFDRSGAEGLTETSVIDLREGEVLGVRWNEEVRVRAPQVV
                     IATGARELFLPFPGWTLPNVMGVGGVQALVKSGASVAGKRVALAGAGPLLLPVAAAVA
                     KAGARVIVVADRTPASRVLEFSASLARRPAELWQGIRYRASFAGTRYRWGTRVVSAMG
                     EDRVREVTFAAGERTWTETLDLLGAAAGLIPNTELARLAGCEVKEGVVVVDAHQRTSR
                     EGVWCAGEPTGVGGVDLSLAEGEVAGLDAAGQKVPAGLLRARDAGRAYARRLDEAFHL
                     GAVERPPPDTIVCRCEDVPCSAFDRSWTMRQAKLYTRAGMGPCQGRVCGAALHAMFGW
                     APDSVRSPLVPTPVASLVSTLESSDATA"
     gene            1961..>2811
                     /locus_tag="VFU75_06905"
     CDS             1961..>2811
                     /locus_tag="VFU75_06905"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU5466602.1"
                     /translation="MQQRDWAGVLPAITTPFKPDLSVDEAALAAHVRWMMDSGCRGIV
                     ALGSLGESATLAFAEKVRILEVCREALGSRGHLVAGIAGLATAECVALAKAAEKAKCD
                     GLMALPAYVYVGDGRETVSHYGAVIGATGLSCMLYNNPVAYGVDVSAEQLAGLCEKHP
                     NLHATKDSSGNVRRVAAIRGALGDRIRILVGMDDVVLESVAMGAVGWVAGLVNALPVE
                     SVRLFDLAISGKSQEAWKLYDWFLPLLRLDTVPKFVQLIKLVQEEVGKGSARVRPPRL
                     QLEGEELA"
BASE COUNT          388 a          895 c         1044 g          484 t
ORIGIN      
        1 attacggatc gcgcgcccgg cttctgccgt caccagatcg tcgagatggg ctacctcaag
       61 agcgcgcatc agatgacggc ggaatcagcg gcgttcaacg tgcagccgcg ggcgaccggc
      121 caggtgctca tcggctcgtc acgtgagctc gtcggctggg atgcgtcggt caatcatgga
      181 ctcgtggccg cgatgctgtc gcgggcgcag cgcttcatgc cggcgctctc gaagctgcag
      241 gcgttgcgga tctggaccgg attccggccc gcgacgcccg acaagcttcc cctcatcggg
      301 cgctgggaga gcacacccgg tctctggatt gcggcggggc acgagggatt gggcatcacc
      361 acatcattgg gcactgcggg gcttctcgcg gatctgatcg ctggccgcac gccggccatc
      421 gctgccatcc cattcgcccc cgggcggcgg atggagccct gatgacccgc acgctctcga
      481 tcatcatcga cggtcgattg ctggaagtgc ccgagggcat taccgtcgcg gccgcgatgc
      541 tgaacgccgg cgaagatgcc ttccgcgatt cggtgtccgg catgccgcgc ggggccgtct
      601 gcggcatggg gatctgcttc gagtgccggg tcaccatcga tggggcgcgg ggtcggcgcg
      661 cctgcctcga acgggtatct cccgggatgc gggtgacgac ccgtgagtag gatgcgggac
      721 gtttcggcgg acctggtcgt catcggcggg ggaccggcgg ggatcgcggc cgccgcaagg
      781 gcgggggaag cggggaagcg cgtgatggtc ctcgacgaag ccccgcacct gggcggccag
      841 atctggcgtc acctgcagcg tgcaacgctt cccgcgaagg ctcgaagctg gctcgatcgt
      901 ttcgaccggt cgggtgccga aggcctcacg gagacatcgg tgatcgatct gcgggagggc
      961 gaggtactcg gcgtccgctg gaacgaagaa gtccgggtcc gcgcgccgca ggtggtcatc
     1021 gcaaccgggg cccgcgagct ctttctcccc ttcccgggct ggacccttcc caacgtcatg
     1081 ggtgtcggtg gggtccaggc actcgtcaag tcgggcgcgt cggtcgccgg gaaacgggtc
     1141 gccctggctg gtgctggtcc gctgttgctg ccggtggctg cggcggtcgc gaaggccggt
     1201 gcgcgcgtga tcgtcgtcgc cgaccgcacg ccggcctcac gggtgctgga attctcggcc
     1261 tctctggccc gcaggccggc agagctctgg caggggatcc gctaccgcgc gtcgttcgcc
     1321 gggacccgat accgctgggg gacccgcgtg gtctcggcga tgggggagga tcgcgtacgt
     1381 gaagtcacct tcgcggcggg cgaacgcacc tggacggaga cgctagacct cctcggtgca
     1441 gctgccgggc tcatccctaa caccgagctg gcgcggctgg cgggatgtga agtgaaggaa
     1501 ggcgtcgtgg tggtggacgc gcatcagcgg acttcccgcg aaggggtctg gtgcgccggc
     1561 gaacccacgg gtgtgggtgg cgtcgacctc tcgctggctg aaggggaggt ggcggggctg
     1621 gatgcggcgg ggcagaaggt gcctgccggg cttctgcgcg cccgggacgc cggccgggca
     1681 tatgcccggc gtctcgacga agccttccac ctcggcgccg tcgagcgtcc tccgccggac
     1741 accatcgtgt gccgctgtga agatgtcccc tgctcggcgt tcgaccggtc gtggaccatg
     1801 cggcaggcga agctgtatac gcgcgccggg atggggccct gtcagggccg ggtgtgcggc
     1861 gccgcattgc acgcaatgtt cggctgggcc ccagattccg ttcgttctcc gctcgtgccg
     1921 acgcccgtgg cgtcgctggt ttccactttg gagtcatccg atgcaacagc gtgactgggc
     1981 gggcgtcctg cccgcgatca ccactccctt caagcccgat ctgtccgtcg acgaggccgc
     2041 gcttgccgcc cacgtccgct ggatgatgga ctccggatgc cgcggcatcg tggcgctcgg
     2101 ttcactcggc gaatcggcga cgctcgcgtt cgcggaaaag gtccggattc tcgaggtctg
     2161 ccgggaggcg ctcggatcgc gcggccacct cgtggccggg atcgccgggt tggcgaccgc
     2221 cgaatgcgtc gccctcgcga aggccgccga aaaggcgaag tgtgacggcc tgatggcgct
     2281 gcctgcctac gtctacgtcg gtgacggccg cgagaccgtc tcgcattacg gcgccgtgat
     2341 cggcgcgacg gggctctcct gcatgctcta caacaacccg gtcgcctacg gcgtcgacgt
     2401 ctccgccgag cagcttgcgg ggctctgcga gaagcatccg aacctgcacg cgaccaagga
     2461 ttcgagcggg aatgtccgtc gcgtcgcggc gattcgaggg gccctcggtg accggattcg
     2521 cattctggtc ggcatggacg acgtggtcct ggagagcgtg gcgatggggg cggtggggtg
     2581 ggtggcgggg ctggtgaacg cgcttccggt ggagtcggtc cggctcttcg atctcgcaat
     2641 ttccggaaag tcgcaggagg cctggaagct ctacgactgg ttcctgccgc tgctccggct
     2701 cgacacggtc ccgaaattcg ttcagctcat caagctggtg caggaggagg tcgggaaggg
     2761 gagtgctcgg gtgcgcccgc cgcggctcca gctggaaggt gaagagctgg c
//
LOCUS       DASUBP010000186        51303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_427569, whole genome shotgun sequence.
ACCESSION   DASUBP010000186 DASUBP010000000
VERSION     DASUBP010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 51303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51303
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_427569"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..266)
                     /locus_tag="VFU75_06910"
     CDS             complement(<1..266)
                     /locus_tag="VFU75_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466603.1"
                     /translation="MVLPLIACGALRDAFSAHAKDAASAAGQSLSAEQLAGWASQVKG
                     MPLQADNLQRLARIWVDYTLFGNSLAGTRSIDDSSLIIEAMWPLV"
     gene            complement(299..3583)
                     /gene="mfd"
                     /locus_tag="VFU75_06915"
     CDS             complement(299..3583)
                     /gene="mfd"
                     /locus_tag="VFU75_06915"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEU5466604.1"
                     /translation="MDRRRDDALMPLDFLLDRMSELPAFDALAATLPRAGQRMGIGGL
                     PGSSAAVLVATLARRFPQRVLTIVAPTPADAERWLSDLEALAPGAVALYPQRESLGGE
                     EQHVEIAGERIETVVALLEGRIRILITTARATVERTGVPGALADARLTLSEAGGPTLG
                     DVAERLVGMGYTKVPTVTEVGQFSVRGGILDVYGFGMAAPGRIEWWGDEVATLRGFDL
                     DTQRSGDPVERVTILPLKEQGRSGAQDSVRSSLLELLPSDALLVVDRESPLRQEVDRT
                     WTEAAHHLEVSRRLGEEPESREALFLSPNRWESLLGGFARLAPEAPDATVRFPISLPE
                     LIDRDLKYLRKLIAGPTRTVILCDNEGQLERLEELLDSGRATLAVGALDGGFVFPGLR
                     VLTDHEIFRRARRLRRARRYRAAVNALGPGAFTAGEYVVHLDHGIGIFRGLETIQVGE
                     ATLDVATVEYEGGDRLNVPLYRIDLLEPYRTSGDPDAPPPRLHRLGATTWQRQRDKTR
                     AAIMAMASELLDLYARRQLAHGYAFPPDTPWQRELESAFLYEDTPDQRKATEEVKRDL
                     ERARPMDRLLVGDVGYGKTEVALRAAFKVVQAGKQVAVLVPTTILAEQHGRTFRERLA
                     DYPVRIEVLSRFRSAPETAAALVKAAAGEVDVVIGTHRLLSKDVLFHDLGLLVVDEEH
                     RFGVRHKERLKALKLAVDVLTLTATPIPRTLHLSLAGLRDLTLLETPPKDRSPVLTFL
                     EPWDDALLEEAIARELDRGGQVYVVHNRVETIATIGARVARLAPRARLAVAHGQMPER
                     ELEAAMAKFATGEVDVLVSTMIVESGLDVPNANTMIVDRADQFGLAQLYQLRGRVGRS
                     HRRAYCYLLVPDLMDARAEERLRVLEHHTDLGSGYRIALRDLEIRGAGNLLGKDQSGH
                     AHAVGFDTYLRWLSEAVLDLKGGGSGGAAPFAPPDVTLDRAAHLSDAYVADDAVKLDV
                     YRRLARAQQPCEIAAVRDELRDRFGPPPEEADRLLLVAELRALGARLGLETVLISGDE
                     ARLTFRRDAHPRLAGLNTALDAVQFEATVRRAQPLSLRLRRLGGEAVGPGLVRALTAV
                     LQDPHP"
     gene            complement(3556..5238)
                     /locus_tag="VFU75_06920"
     CDS             complement(3556..5238)
                     /locus_tag="VFU75_06920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HEU5466605.1"
                     /translation="MIFLHPLALFGLAAAAIPALLHLRQRRTPPVLDFPAVRYLADAE
                     RRTARRLRLRHLLLLLLRTLLIASIVMAAARPQVRGWGRATGHEPTAAVIVLDNSLSS
                     GVVVDGRRVLDGLAAAAHAVTARAGSGDRLWLLLADGVVRRGTPAELDAAIDDARPEA
                     GRMDLSEAVRRAVAVAAGATLSDRDVYVISDGQASALSGPPVPAGSVHVIALEPRSGV
                     APNRGVGDLRLVDTRLLIPIVGTPGGKSGTLTVTYRDRVISRGLAKPGDTLAIALPAA
                     PVGWWTGSVDLEADELRGDDRRLFVQRVHPPARVSAAPTAGPFVAAALDVLLNADRIA
                     AGKDVVIATLPTGRFAVVPATGDPASVGAVNRALGLRGGHWRFGVPATPGRVSVRGSG
                     VSGLGGVPVGRRLELTGGDPATVLATVNGSPWLVREGSTLLIGSALDTAWTTLPATPS
                     FVPFMDALVNVLAQGEAPVSDTTGAPGMQFRTNGRDTVGALVLGPDSRESDLTPATPS
                     MFSRALDAESFSSSAFRDAAFTGSARGDAGGLLLMLALLLVAGEWIVAVTTR"
     gene            complement(5235..6119)
                     /locus_tag="VFU75_06925"
     CDS             complement(5235..6119)
                     /locus_tag="VFU75_06925"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEU5466606.1"
                     /translation="MATGSRLDLLDPREVAQLGGLEITAAGVVEGFLAGLHRSPFRGF
                     SVEFTEHRAYQPGDELRYLDWRILGRTDRPYVKQFEEETNLRAMILVDVSRSMAWTGS
                     PDRLTKRAYADRLAAALSLLLLRQRDATGLITFDDRIRQALPPRVKSGQWHRIVRGLI
                     ETPDGEGTAAEEALRQVTARLARRGMVVLISDLLFDRDLALTALRYLRHRGHQVTVMH
                     LMDPAEVDLAGPPEVRYQDLESRGSVVVRPRELARSYRETVRREIAAWRSACRRHEIA
                     YHHVTTDQPFGVALRRLA"
     gene            6288..7403
                     /locus_tag="VFU75_06930"
     CDS             6288..7403
                     /locus_tag="VFU75_06930"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HEU5466607.1"
                     /translation="MLIHHAIITALALAPSPAHPAAPRGPGHDGKGRVQVWTENGNDP
                     YRTGDQARVFIRGEEDAYITVFRVDTDGRIQVLFPRAPWMDNYVRGDHEYEIEQSRSS
                     NAFAVDDDPGVGYVFAVSSPDPFTFDPVTDGDHWDYRAMDGGRIQGDPYVALTDLAGR
                     ILPSNDEDWDYDISPYYVERHYDYPRFLCYDCHAFASFGSWDPYAATCPRFRIVAYDD
                     PVYYPYHYYQGRAVVVARPLRPEPRFVFKDRDGAPDEQFVTVTRDRSREEGRNRGASG
                     RDFGGPGRLPAPSRTGREYEPQQPDAQHPDHRQPDQRRRPPEQRPAQPPPPRQQPKPN
                     AEPRRGPPPRPAPAAKSPPPKEQPPKNQPELRRRKPN"
     gene            complement(7400..8467)
                     /gene="rho"
                     /locus_tag="VFU75_06935"
     CDS             complement(7400..8467)
                     /gene="rho"
                     /locus_tag="VFU75_06935"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEU5466608.1"
                     /translation="MPPITGWYDPTPKGGALRLAANGYLPANGDPPVRLPGLRRGDLV
                     VLEGGRVTSVNGAPPAGLADRPEFAKLGAVHPERALRLETDKASSPRTAAIQRVVDLV
                     CPLGFGQRALIVSPPKAGKTVTLQAVAEAVAINYPGATLMILLVDERPEEVSEMVDWG
                     RGEVVASSFDLPHERHVAVADMVFEHARRQVELGRDVVIVLDSLTRLARSHNTAGGRG
                     SGRTMSGGLDANALAKPKALFGAARAVPQGGSLTVIATALVETDSRMDDVIFEEFKGT
                     GNAELRLSRELADRRIYPAVDVAASGTRREELLGDAATVEAKQKLRRGLVDLPPEQGI
                     QQLLSLMKRAPTNAELLAGVR"
     gene            8571..10370
                     /gene="lepA"
                     /locus_tag="VFU75_06940"
     CDS             8571..10370
                     /gene="lepA"
                     /locus_tag="VFU75_06940"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HEU5466609.1"
                     /translation="MRQSLIRNFCIVAHIDHGKSTLADRLIEHTGTLDKRLMRAQVLD
                     TMDLERERGITIKLNAVRMNYHAQDGEIYELNLIDTPGHVDFTYEVSRSLAACEGAIL
                     VVDASQGVQAQTLSNLFLALDAGLEIIPVLNKIDLPGAEPDRRAAELHDLIGSRPEEI
                     LRISAKEGTGVPELLEAVVKRIPAPKGDPAAPLRALIFDSYYDRFRGAIPSIRVVDGV
                     LKAGMQIVFGAHKADHYQVDEVGNLQLGHKPVEQLAAGEVGYVVANVRGVQETRPGDT
                     ILDVEHSDVPLLPGYRDIQSMVFAGLYPTNAEQYEDLRDALEKLQLNDASLHYEPESS
                     VALGFGFRCGFLGLLHMEIVRERLEREFNLDLISTVPNVEYHVYKTDGVKELVENPSL
                     MPHGSTVDRIEEPYVKARVLAPSEYIGAMMKLGQERRGVYLGMHYVDPTRVEFSWEFP
                     LAEIILDFYDKLKTISRGYASLDYEFLAYRRSELVKLDMLLNGDAVDAFSVIIHQDKA
                     YEWGRKIAEKLKELIPRQLFEVVIQAAIGTKVIARESIRPLRKNVTAKCYGGDVTRKR
                     KLLEKQKEGKKRMKQVGTVEIPQEAFLAVLQVD"
     gene            10363..11373
                     /gene="waaF"
                     /locus_tag="VFU75_06945"
     CDS             10363..11373
                     /gene="waaF"
                     /locus_tag="VFU75_06945"
                     /inference="COORDINATES: protein motif:HMM:TIGR02195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008920 - lipopolysaccharide
                     heptosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase II"
                     /protein_id="HEU5466610.1"
                     /translation="MTSAVVVQTAFLGDVVLTTPLLRTLAVRHGPVDVVTTPAAAPLL
                     DTHPAVRQVIRYDKRGADRGWGGLTALARRLREAHYEVAYVPHRSLRSALLALWARVP
                     RRVGFRDGWRSLYTESRARPAGGHEVDRLLALAGDPTAIRTPSIGLTPADHGAAAAFV
                     EAEALGSDVVALAPGSIWGTKRWPHFTALAGRLAGSHAIAVVGGPGDGELAAAIVDHV
                     RSAGGRAASGCGKLTLRESAALLARCRLLVTNDSAPLHFAQAVGTPTVAVFGPTLPAF
                     GFGPLHPGDRVAEVTDLTCRPCSKHGPAACPLGHHRCLRDLPVERVIAAIEETGALRR
                     RD"
     gene            11351..12319
                     /locus_tag="VFU75_06950"
     CDS             11351..12319
                     /locus_tag="VFU75_06950"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEU5466611.1"
                     /translation="MRYVVGIDIGGTNLVVGTIAEDGSELLGEVTEPTHSEEGADAVI
                     ARIGRLVKQSTAAAKGKEIVGVGIGSPGPLDTHRGIVLFTPNLGWTNFPLRDRVTKVT
                     GLPAELDNDANCAIYGEWWRGAARGAKAVVGLTIGTGIGGGIVLDGKIYHGVSDVAGE
                     IGHTTIDANGRRCKCGNYGCLEAYASGPAIAARAAEGIESGIDTKLGKYVNGDLSRLT
                     AQTVYQAAHDGDQYALDVVNDTAKLLGAGVANMINIFNPDVVVICGGVTLAGDRLFDP
                     LRREVHRRAFKDAVKACRIVPGELPGTAGVFGAAATFIEKHRGGRA"
     gene            12316..13236
                     /locus_tag="VFU75_06955"
     CDS             12316..13236
                     /locus_tag="VFU75_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEU5466612.1"
                     /translation="MKRIGVVGALVWDTIYGRDPAQPAVEEWGGIAYALAGLDAALDD
                     GWEIVPLIKVGRDLAEPAGRFLRSLRHVAPGARFVLVPEANNRVTLRYETKERRCELM
                     SGGVPGWTWGELGPMVGDLDALYVNFISGFEMSLETARMLRRGFSKFIYADLHSLFLA
                     KDANGLRTLQPLVDGPGWFGCFDAVQLNEEEMGQLGPDPLAVAAGALAAGTGVLCVTL
                     GRRGAAYFTGSPVRTARVSSGETGAAPFEDGDPTGCGDVFGATAAAALVQGADVEAAL
                     QLAARRAARNVSFRGATGLRDHLLGKLAGV"
     gene            13233..14138
                     /locus_tag="VFU75_06960"
     CDS             13233..14138
                     /locus_tag="VFU75_06960"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5466613.1"
                     /translation="MTRLVDVPQQFDDRSFEQFASSFAQGAAGGERLLFDAHATEWAS
                     PYGFVGLLVAGQAVQSAGRERPLLTIPANPRVLSYWTRAGFFQQAATLFEIHGKVPKL
                     KTSEESDLLLPVTAVRAAEDVHQVVSHIQQRATAILTSQLGIDPKATVGFAMALSEAC
                     QNIVEHAGTGGWVAVQRYEWKRRLSKKVVVIAVGDAGVGFRHTLEPTQAKRFGDRWGD
                     GAALEAALIQGTSRFRDPGRGQGLKGIRSYLSRWDGKIAIRSGTARIAIAPSWDDDAP
                     LTEGLSAFPGSQVLLIIPEQGTGTS"
     gene            14135..14560
                     /locus_tag="VFU75_06965"
     CDS             14135..14560
                     /locus_tag="VFU75_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466614.1"
                     /translation="MIAPIDVHAVLQESVPGPYADLVTRHTGRAVRERIERAIADATV
                     ASMDFTGVRCIDYSCADEIVAKLLRTVQILLLQGITEAHRLAIEPVLQGHGLAVVIQT
                     GTLLEVLGPPDAAEVICERLLESRLAERTAGGTLALTAA"
     gene            14557..15714
                     /locus_tag="VFU75_06970"
     CDS             14557..15714
                     /locus_tag="VFU75_06970"
                     /inference="COORDINATES: protein motif:HMM:NF013237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aspartate aminotransferase family
                     protein"
                     /protein_id="HEU5466615.1"
                     /translation="MSRRRLGLSTSAIHGAPEPRPDWSPVTTPIYQSSTFTNPIGSTQ
                     EVLYTRYGNNPNQIAIAKRLALLEGAEAAIFVGSGMGAAALAHLAILRPGDHLLSSEW
                     IYGGVYRLFTQEFGRLGIEVSFVNPVESRSWKRALKKNTRAVFVETPTNPVMRVLDID
                     SIGTLCKAEGLALLVDSTFASPYNFRPLEHGADVVIHSATKYLNGHSDVIAGAIAGTE
                     AVVEEVRKLMWVYGQALDPMAAWLIERGLKTLSVRMERHNRNGQQVAEWCAKHKAIAA
                     VHYPGLPTHPDHQSAQATMQGFGGMLGIELKGGVRAAERFLRGLTIAAHAPSLGGVET
                     LVSEPRLTSHASLTADERAKAGIPDGFLRFSLGLEDADDLIGDFAQSLAQL"
     gene            15714..16415
                     /gene="ftsE"
                     /locus_tag="VFU75_06975"
     CDS             15714..16415
                     /gene="ftsE"
                     /locus_tag="VFU75_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEU5466616.1"
                     /translation="MIKFTNVFKAYPKGGLALKDATFHVAKGEFAFLTGHSGAGKSTI
                     LKLIYAEERATEGEVRVSGFNLADLPPRQVPYLRRRLGIVFQDFRLLEDRTAEENVGF
                     ALQVTGARRAGIEARVMRVLTQVGLAAKATAYPRELSGGEQQRVAIARALVNEPTVLL
                     ADEPTGNLDERATRGVFQLLRDINASGTAVVMATHDLDLVRQAPYRVIELQDGALVYD
                     SAVDEPRGEQSGGAP"
     gene            16412..17272
                     /locus_tag="VFU75_06980"
     CDS             16412..17272
                     /locus_tag="VFU75_06980"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HEU5466617.1"
                     /translation="MNFVMREAMLTFRRAPLLSALSVTTIAFSLFVVGLFALVAVNVQ
                     RALSQVAERAEVVAYLLPGTPIETITHALKDIEAFPEVTNASYVSEDSALARARRELV
                     ELQGPLRELERNPLPASIEVTLKPGDRDADHVGAVADRIRDFAFVDDVRYGRDWIEKL
                     DKLRSVLAAVGLAVGIAFAAVAVIIIGATIRMTVLQRGREISIMRLVGATDWFVRFPF
                     LLQGLIKGAAGGLLAVGLSYAAYLLSNRWLMMRTEFFNPEQAAAIVAGGMVIGLFGSW
                     ISVGRQLRKV"
     gene            17362..18546
                     /locus_tag="VFU75_06985"
     CDS             17362..18546
                     /locus_tag="VFU75_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEU5466618.1"
                     /translation="MTLLLDAVLVLALLSPLSAQKPGSFDQQMRDNQRRLEDIRRQRS
                     DAENELESLRTQVHSIEDEIANLERQKETTNRIVNEIDRQIGELGSQVSQTTVDLILA
                     QDALAEKHAVLERRIVDIYKRGPLYDWQVLLTSESFGDLLSRYKYLYLVSRQDRLLAN
                     DMQKLEDRVARDRHNLLDARDALGRRRNERADELTRYSDLEARRTQDLRETRRGIRAA
                     QLKVTNSEKEENRLTDVIARLERARRDALARGAATAKATITTASLGTLPWPVSGNLLY
                     RFGPAPGPDNTQITWHGVGIASPAGTPVRAVASGTVVDVGVRGTYLTTVILQHGGGYY
                     TIYATLSDATVHLGDPIVAGEVIGHVGGASTDQGAHLHFEIRGPDQIALDPLNWLKRQ
                     QP"
     gene            18602..19216
                     /locus_tag="VFU75_06990"
     CDS             18602..19216
                     /locus_tag="VFU75_06990"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5466619.1"
                     /translation="MNHTTSTPDRLLNAGWKLFAQQGYDRTSVRAITRAARANLGAVT
                     YHFGSKEHLYHAVLERLFREMGDRLSRAAAGNGDPGARIRAIVRTMFEFFGEYPDAPR
                     IVLQQVTRGGAVPDAVATRLRANLAIIAGVVAEAQARGLFRPAEPLFGAFSIISQTVW
                     FAVVRRTIPQVSHALGNPDLADRFEHHIAEIVTRGLARNPESMP"
     gene            19213..20085
                     /locus_tag="VFU75_06995"
     CDS             19213..20085
                     /locus_tag="VFU75_06995"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5466620.1"
                     /translation="MNRFLRLCLAGLAPAVLVACHGDAAQSRIAGTVEIRQVRVSPLT
                     PGRLVRLFKDEGDSVHAGDTIALLEQPGLHALIDERRARAASAASRTGEVGAAIAEST
                     RAANDLIRAEPLRAQGIVSAQQYEALTAAAAVAAAHLQSVRAAPTDEAAAQAALQGTV
                     AIRNELVLTAPVDGVILTRYAEPGEAVSTGAPVVSIGEVTRPWVRAYLPEPLLARIRL
                     GTRVRVHADGYADSAFTGTVAEIAPQAEFTPRAALTERERADLVFGIKVEVGSAGGRL
                     KAGLPVTLDIPLLP"
     gene            20082..20816
                     /locus_tag="VFU75_07000"
     CDS             20082..20816
                     /locus_tag="VFU75_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5466621.1"
                     /translation="MTAAVITRGLRRTYGALVAVDHLDLEIEPGEVFGLLGPNGSGKT
                     TTIRMLCGLVAPTSGTAEVAGIDVTRAPEEVRNRIGYMSQRFGLYEDLTVAENLDFYG
                     AVYGLQGAAQAVRAGEVVQFLGLDQRLGQLTGQLSGGWKQRVALGCALMHRPTVLFLD
                     EPTAGVDPAARRSFWAAIKNIAGHGTTVIVTTHYMDEAESCGRLGMMSGGRLIALGTP
                     AEVARQVSGGGTLEDAFIRLQEQVER"
     gene            20816..21925
                     /locus_tag="VFU75_07005"
     CDS             20816..21925
                     /locus_tag="VFU75_07005"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5466622.1"
                     /translation="MGHLRPMIRKEFIQMRRDRLTLGIMVAIPVVQLLLFGYAIQTDV
                     RHIPTAVFDDSRTPESRDIVAAFENTGNFTIIARVASRAALDDAIARGSAQAGIVVPH
                     DYPEALAGGRSARIQVIVDAEDPLSSQSALAAASGVGEVRNLAILSAAAGRTILPIEV
                     RVRPRYNPGLRSAVFIVPGLVGVILTLTLVLVTAMAIVRERERGTLEQLIVTPVTRTE
                     LMIGKIAPYVAVGLVQMTTVLFLGRWVFDVPLTGSLLLLYGVTLVFIVASLAFGLLIS
                     TLVRTQAQAIQASFFFVLPNILLSGFMFPRAAMPAVAQWIGLALPLTYFLKILRGILL
                     KGVGIESLWPEVAALAAFGAVVITLAVRRFHKTLD"
     gene            complement(22041..23450)
                     /locus_tag="VFU75_07010"
     CDS             complement(22041..23450)
                     /locus_tag="VFU75_07010"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU5466623.1"
                     /translation="MAESKPSRLLLLGAPEARPEGLERMLVRAGFAVLDAETAADGPP
                     HVILLGATPNDPSLIERVRDMVKNPRHDGASIVVLLTGGTALHTAALLSAGATDVIRA
                     PIDLTEVEARLASDRRRRATLSSARRALRTREALFDIFQEVSGALRPEQVFETLVRRV
                     GEAFGLTHCSFVLTGPGGAQGRVVAVYEDPKVRDLKVHLDRYPEIQEALKTRAPVVVS
                     DVHQHPLFAAIRRHWEEQRIDVKVRSSAALPVFVNGRAAGVFFLRTERGEPELDPTDV
                     DFANTIAQAAARVLENEERRSAMFRRQVSAGTTDALTGCGSLDALDQRLREEFERARR
                     YTLAFSLVLLDIDALRTINETSGQTVGDEVLFDLGAMLQREIRAPDFVARYGGDEFAL
                     ILPGTALAGARGFVERIRRVVDAHTFPHLGPVRSPSLTAGLVTFPHPDAFEPKDLIRL
                     LEESLAVGKKAPDRIGSTS"
     gene            23526..24173
                     /locus_tag="VFU75_07015"
     CDS             23526..24173
                     /locus_tag="VFU75_07015"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:TIGR00080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HEU5466624.1"
                     /translation="MSRPAPGTAGPRRRMVREQIARRGIQDARVLLAMDRTPREWFVP
                     DESSADAYADEPLPLIAGQTISQPYVVALMTALLAPTRHDRVLEIGTGSGYQTAILAR
                     LAAEVYTVERIPELLVTAEARLLRMGLTNIQTLIADGARGWSAAAPFDGILVAAAASQ
                     APPPLLEQLAPGARLIMPIGDQRRQELTVFTRLDSGVRAHGFGGVRFVPLVSPLS"
     gene            24170..25024
                     /locus_tag="VFU75_07020"
     CDS             24170..25024
                     /locus_tag="VFU75_07020"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016888 - endodeoxyribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEU5466625.1"
                     /translation="MNDTLALLGAHVSTDGGVSTGPARGAEVGARAIQLFTKTPNQWR
                     EPPIIPDEVERFKAEMARTGIEVAVAHDSYLINLASPDDKLRAKSLKCFTGELERCKS
                     YGIPYVVSHPGNFMDDHDAGLARNAAAYAAALAEVPGDVGVLIEGTAGAGTALGRSFE
                     ELRDLRNGMPEAVRHRVGFCLDTAHMHAAGYDVVGNITGVLDEFDRVVGLEHLKCFHL
                     NDSKAAPGSRLDRHDWIGEGTIGAEPFRRILQDARFAKVIKIIETPKGDDPVHHDRRM
                     LERLRSYA"
     gene            25059..25409
                     /locus_tag="VFU75_07025"
     CDS             25059..25409
                     /locus_tag="VFU75_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466626.1"
                     /translation="MTRSRTRRSWLKPALAALLLFLQVTGAPVVGIAHAREPGPAPVS
                     LESGHSDRCPVLHNELTCILCQYAGSLVVTRPAQPEPDLGRIAFAPAAVVSRPRAVER
                     PAPCTSPRAPPALS"
     gene            25491..26780
                     /locus_tag="VFU75_07030"
     CDS             25491..26780
                     /locus_tag="VFU75_07030"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU5466627.1"
                     /translation="MVTRSSFWLIAVGTALAIPAHAQGPATPPALTLTRRQAVDTALA
                     HNPQLTVAREQLAQARAQATETAAFPDPTLNGDLNGQRSVTGFKTNTGSDIGVGLTLP
                     FPTKFVLLNKVGNANVDAVAFNYDQLRQQTASQTAQAYDALIVALRHRADLQLGDSLA
                     RDFLKKTQVRFDAGTAARLDVLKAQVDLGQAENDLLANQRDLANARAALNRLLGRPLG
                     FGVEAAESLSVPPALPAVDSLISLAQLRRPELQGLASQQRGASAATALAQQYFLPDLN
                     LSVAKNMTQGTPSTYTTGIGISVPLFFWNHQRGQVAESRHHERELAAAYSDLEAQVEQ
                     DVRVTYATAATALAQAVYLRDQLLPEAHRAYDIALTSYGLGGSSALDVLDARRTLLDA
                     ESQYADALGAANDARADLERAVAGPFDAASAGVPNVH"
     gene            26770..27906
                     /locus_tag="VFU75_07035"
     CDS             26770..27906
                     /locus_tag="VFU75_07035"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5466628.1"
                     /translation="MFTNRLTRLLTAAGMAALLATGCGRGAAKDPDHAGTFGLTENQR
                     SKIHLAPVADSSFHPSVSVSGTVAFDGDHSTQVLAPISGPVSRILVEPGAQVTAGQPL
                     AYVSSPDFAAALAAYRKANATATNLIRIATRDSVLFANDALARQDLEQAQTDASSAIA
                     DRDAALDQLRAIGVDSAGLAAIRDNRNATIPQGVIRAPLPGTVVERLITPGQLLQAGQ
                     TPCFTVAGLSTMWVMGNVFQNDLPDVAVGDPADIAPGAGARVYHGRVDYVGALVDPDT
                     RATSVRILTGNPDQGLKKDMYVSVTIHSHRARKGLLVPVSAVLHDDQNLPFVFVAKAD
                     SSFERRQVGLGNRIGDRYEITSGLSAGDEVIAEGGLFLQFAESQ"
     gene            27903..31028
                     /locus_tag="VFU75_07040"
     CDS             27903..31028
                     /locus_tag="VFU75_07040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CusA/CzcA family heavy metal efflux RND
                     transporter"
                     /protein_id="HEU5466629.1"
                     /translation="MSEQLPPPAHEARTRSAINRIVFSSLRQPFLVGLLTVTFAGIGI
                     WSFSRLPVDAYPDLSPTMVEVITQWPGHASEEVERLITVPVEVEMNGLPRMQVIRSIS
                     LYGLSDVTMTFADGTDDYFARQQVFERIPDISLPDAVTPSVSPLFAPSGLVYRYVVQS
                     PDRSAMDLKIIDDWVITRQLKAVPGVADVSPLGGETFQYQILLDPTRLAGAHLSISDV
                     ESALAANNGNAGGGFYSEGGQFYYIRGVGRMATLDDIGNTVLANHNGTPILVKDVGQV
                     VVGHAPRLGQFGYNGQNDAVEGVVLMRRGEQAQTVLQGVEAKVRELNQSVLPKDVKVV
                     PFYDRSDLVALTTHTVEGNLARGTLLVVVVLVFFLYDVRSGLIAGVTIPLALLFAFLC
                     LDLRHIPANLLSIGAIDFGILVESAVVMVENIHRQLALRHGTSYRIHEVIADAAAEVD
                     RPIFYSMAVIIAGFLPIYVLHGPSGRLFTPMASTTIFALIGSLAVALAVLPVLCAWFL
                     RKGVRERRNAAFEWLRERYTRGLDWCLARPRATVAGAGVLFAAAALLIPGIGAEFMPH
                     LDEGAIWVRATMPYTISFEESAKIVPQVRAIIRSFPMVTVVTSEHGRPDDGTDPTGFF
                     NAEFYVGLKPYDEWKGEIRTKAQLIAAIDSRLRAFPGIIFNYTQPAEDAVDEAETGLK
                     SALDVKIFGPDLDTLETLGRSLKKALEDVPGITDLTLVQELGQPSLTITPDRNRIARY
                     GLNIADINALIEAAVGGQAATQVVQGERSFDLVVRLEPQFRETSEAIGEIPVPTPGGQ
                     NIPLKDLATIQVTNGASFIYRQDNARFIGVQYSVEGRSLATAVRDAQARASARVTLPP
                     GYRVVWGGEFQEYTASRRQMMVIVPVTLGLIFLILFALYSNLKFPLITVAAVLLSAPV
                     GGLLALWLTGTAFSVSSGIGFLALFGVSVQTAVVYISYANELRGDGMPIAQATREAAL
                     LRLRPIMMTALVAALGLLPAAFATGVGTDSQRPFAMVIVGGMLSQLVVSVFLVPALYL
                     LVAREGDRLEV"
     gene            31214..31693
                     /locus_tag="VFU75_07045"
     CDS             31214..31693
                     /locus_tag="VFU75_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466630.1"
                     /translation="MNAFDVRPALAPAFSVTAALPLDLPWRASAGLDVSTSDVRREDL
                     GGATQSITHLTTAALTVGLSRSLAPWVSGSIRVGALKYLPSQDLGLFQDGGPFFPFAQ
                     IAFDLSPSFASRYGVGLEMNGDVHKFITDALRVEGFSEARPVLRLALGVTWTPGSHP"
     gene            31690..32328
                     /locus_tag="VFU75_07050"
     CDS             31690..32328
                     /locus_tag="VFU75_07050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466631.1"
                     /translation="MKRWLGVVAVALVACTVPDVPEHSGGYGFDSVGDGSGLVFHWTG
                     DRLPVRFWVDPRGNMSALVQNAIDVWQNQFMYGEFTGVIVSDSAHADVQIVWADSAAP
                     PATPDPGPPVKACGGETQNDSISGGRIYGALHSSISLLTLSGATAGQVEACVRRTVIH
                     EMGHSLGILNDNAPDSTDIMYFTPLVPVPSAADRTTMQVLYHTAPTLLPPVR"
     gene            32409..34679
                     /locus_tag="VFU75_07055"
     CDS             32409..34679
                     /locus_tag="VFU75_07055"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF007233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HEU5466632.1"
                     /translation="MATRRQEALEYHAQGRPGKIQVSPTKPFKNQRDLSLAYTPGVAE
                     PCREIAAKPEEAYKYTIKGNLVAVVTNGTAVLGLGNIGALAGKPVMEGKCILFKAFAD
                     LDVFDLEVGSENPDDVIKFCQLLEPTVGGINLEDIRSPDCFYIEERLRETLSIPVFHD
                     DQHGTAIISGAALLNALELVGKAIGDISAVFCGAGAAAIATAEHYVRLGVKRERITLC
                     DKDGVIHAGRTDLDPYKKKFAQPIDARDLRDALKGADVLVGLSVAGAVTATMLAGMAP
                     KPIIFALANPTPEIMPDEARRARPDAIIATGRSDFPNQVNNVLGFPFIFRGALDVRAT
                     TINEDMKMAATRALAALAREEVPDSVMRAYGIERLRFGPDYLIPKPFDPRVLLWVAPA
                     VAWAAVGSGVAGRVIDVDEYRQELEGRLGRAREVMRGFAAHLQEDPQRILFPEGDHPK
                     IIRAARVLADEGSAVPVLLGDPAEIRRAAEDAGVTLEDIEIVNPAEAADSEGLARAYW
                     ERRQRRGVTLREARARVRDPLYHGLLLLKTDRADALVVGADMYYPDAVRPALEVIGAA
                     PGRKHVSGIYMMVLPQDVFFFADCTMNIDPDAETLAAIASTTAEFVWRLGIEPRVAML
                     SFSNFGSVPHDGVTKVRNAVALLHEREPSLQVDGEMQADTAVVNSILTKTYPFTKLRG
                     AANVLIFPGLDAANIAYKLLNRLGGAQAIGPILVGMSRPVHVLQRGADVDDIVNLAVL
                     AAVDAREHRRPVRPHT"
     gene            34702..36306
                     /locus_tag="VFU75_07060"
     CDS             34702..36306
                     /locus_tag="VFU75_07060"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HEU5466633.1"
                     /translation="MTTPRTGRRSGASIEFSGLAPEHHVHWNYGPAELVEHAIRRKEG
                     MLADGGPFCAVTSPHTGRSPNDKFVVKEASSEAKVWWGKVNQPIAPEHFDRLHADVLR
                     HLNSQEIFVRDLFAGADPDYRIPVRFVTPNAWQALFVYNMFLRPSQPELAAFTPEWQV
                     LHAPELHADPKVHGTKSSTFIVVNLGKRIILIGGTRYAGEMKKSIFTILNYLLPNRGV
                     LSMHCSANVGEAGDVALFFGLSGTGKTTLSADPERGLIGDDEHGWSDRGVFNFEGGCY
                     AKVIRLSREGEPEIFQTTRMFGTVLENVVVDPETRRIDLDSAAITENTRSSYPIHYIP
                     NHVAGGQGGHPTHIVFLTCDAFGVMPPIARLTPAQAMYHFLSGYTAKVAGTERGVTEP
                     KETFSACFGAPFLPLHPNTYAELLGAKIAKHGVQCWLVNTGWTGGGFGVGQRMRLSHT
                     RCMVRAALAGKLDKVANVTEPAFGLSVPQHVPGVPDEVLLPRKTWSDAAAYDAMAAKL
                     AGLFKNNFVQFQDQVPAAVREAGPRI"
     gene            36303..37580
                     /locus_tag="VFU75_07065"
     CDS             36303..37580
                     /locus_tag="VFU75_07065"
                     /inference="COORDINATES: protein motif:HMM:NF039904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU5466634.1"
                     /translation="MTASSIGAPLEVVRDPLWNNIRLEPDALTVLDTPAVQRLRYVRQ
                     LGHAFLVYPGATHSRFEHCLGAYHLARRALEHLSHGDVAVHPRDATAVRLAALLHDIG
                     HYPFSHALEEAGLPKHEALARRHLTGGQLGEVLGKIGIDPEILLSLIQGRGTVPLAGM
                     VAGSLDVDKLDYLSRDAWMCGVPYGVIDVDRLLSSLTIVEKSGRPTLALHEKGLAALE
                     SLLFAKYQMYRNVYWHHAVRSATVMFKRAVTDAIAAGRLSTDAVADATDDGLIHELSA
                     NDPAGLARSLRDRRLAKRALDLPATDLPSPADWPAADPALVAQVEDRLAAELKLAPGD
                     LFIDFPAKPEMLDLRLPLVTRDGEVVELEGQEAAERLGLPRVSAELVRSARRLRVFVR
                     EPRSVPERAVVNLVTLPGAEVASRLAGDRSLLS"
     gene            37630..38427
                     /locus_tag="VFU75_07070"
     CDS             37630..38427
                     /locus_tag="VFU75_07070"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU5466635.1"
                     /translation="MNAKLRIAAGATLLGLLALGVADLSLGASVPRYLDLERTNAELL
                     TQIRETQGTLQILSDSVESVSRRAQVLRLLAGVSPLDSGVDLPGTDPSWAWAQRDSPA
                     AWSRDVTRTSAAPTTPEELALAGTLPVAITLTAIPPGRVSSPFSAERLSPVVDTVRPH
                     QGVDFAAPPGTPIRAPAAGVVVEVQWEEGYGEYLTIDHGHGVVTRYAHCSDILVAPGS
                     RVRRGQKIALVGSTGEATGPHLHFEVWVDGRAVDPQRFVAPGDVVTD"
     gene            complement(38468..39247)
                     /locus_tag="VFU75_07075"
     CDS             complement(38468..39247)
                     /locus_tag="VFU75_07075"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEU5466636.1"
                     /translation="MHITCAGRTDVGIIRSGNEDNFLMVPDKGVFIVADGMGGHAAGE
                     VASEMAVRVIAREVGSLQGLNDEQVAERMRLSIRAANGAIFERTLTEHDKRGMGTTVT
                     ALTLFANRFLIGQVGDSRAYLLRDGKLAQVTKDHSYVQEQVDAGYLTPEQARTHPYSN
                     VITRCVGANADVMPDVYVGSVRERDLFLLASDGLTGMLEDPQLAEIMKNGLQPQEQVD
                     SLIEEANRHGGLDNITAIMVRIDQVDTPAGGRATEENTLPG"
     gene            complement(39256..40653)
                     /locus_tag="VFU75_07080"
     CDS             complement(39256..40653)
                     /locus_tag="VFU75_07080"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HEU5466637.1"
                     /translation="MALLEMGGRKFTIPAGEVVLGSAADCAIQLTGPGITPKTAILKG
                     LPDGQVSIRKVDPQAIIAINGVNLGAEPTPLLHGDKIETGGHELTFVDERRSGSTQYV
                     AAMDVSKAAAAAKASAGAGKPTANTGGRIVSLTDGREYAVTGASLVFGREAGCDVVVT
                     GKDVSRRHAEILTTPKGYMVVDSSTNGTFVNDEKIEGQRLLMRADVIRIGDEQFRFYA
                     DAAPAVQAPPSPPAGPAASPPQAAPQTPPPPSGAGHRLNNTSFGMAAAPRPSATAPST
                     PAGSPAPAAMGNFLFRTGPLKGQRYQIKTPVLNIGRADYNDLVLADPSVSTSHAKLQR
                     REGVWMLVDLDSTNGTFVDGEKVQGESPLSPGVMVRFGDVQAVFEPTDDAMGTAKGGG
                     TKVIEAFKPGSAAPAPAPKAAAPTPPPPKAPAPVPAVKRQPVPPPVPAKKKGCGASAA
                     MFVLGGASLLYWILR"
     gene            complement(40727..41821)
                     /locus_tag="VFU75_07085"
     CDS             complement(40727..41821)
                     /locus_tag="VFU75_07085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HEU5466638.1"
                     /translation="MALALTPRLLRRGLALFAIISLAGVVALLFYGDNLRAFLDALAS
                     IHWRWVGIGLALASMDWVGGGTRLWVVARHIQPGVRLRDMIVAGGMSAWGGYLTPFQT
                     GAGPMMMWTMKRAGVRLPAAFTATFMTFVATVAFFAIAGPLAIFFGAGRSLAKHHIVL
                     GIGLYDLFRVSLGVFVFLGLLMLFLMVFPRLAKDLVHWLAGRVGKRSRRVAAGMEQLR
                     EGIEYGHRCFVAFGSPKGWLATFWAIVLSAPSHANKLLAGYVAMRALGIPADFVEILL
                     VQTLITFVLYFAPTPGGSGMAEGISTVVMSIYVPRPLIPSYTLVWRFINSYATVIVGA
                     LIFWGWLRKGLIGMEEAVSTDQRPEITDGA"
     gene            41855..44662
                     /gene="uvrA"
                     /locus_tag="VFU75_07090"
     CDS             41855..44662
                     /gene="uvrA"
                     /locus_tag="VFU75_07090"
                     /inference="COORDINATES: protein motif:HMM:TIGR00630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU5466639.1"
                     /translation="MPAPPDQVVLRNVRQHNLKGITVSLPRRAFTVITGPSGSGKSSL
                     AFDTLYAEGQRRYIESLSSYAKQFLERMPKPLLDSLEGLSPAVAIEQKNPTTSSRSTV
                     GTATEIYDFLRLMWARTGTTYCRNCGNVVKADTVQDVVDGVVGSRESGVVDSVTVAFP
                     LPGAGDRADIEIAAQLRAAGFVRVQLDGEVTRLDTDDAESRIRAAKEVLILVDRVSAS
                     PANRGRLTDAVSTAFAEGDGVALVLTDGGHRRRFSEQPACSNCGRPAPVPTPNLFSFN
                     NPRGACPGCNGFGAVLKYDESLIIPDQRLSLAEGALDPWTKPRYEGRRRILRDAARNR
                     GVSVDTPWQEIPAGDREFFLHGTKGRFVGMFPFLERLEEKRYKQYIRVFLRQYQLAKT
                     CPQCGGARLQPDALAVKVAGKTVAEVSALTATALTTWLKELSLPDFQQAIAEHILGEL
                     VARVQFVVDVGLGYLTLDRQTRTLSGGEAQRIALSNALGAHLVDTTYVLDEPSIGLHP
                     GDTDRLLGLLRRLADAGNTVVVVEHDPAAMAAADYMVELGPGSGENGGQVVYQGPAAR
                     VREAGTLTGQYLSGEKRIGVPSARRPAGPRWLTVRGARLHNLEGVDVRIPLGTLTVVT
                     GVSGSGKSTLVHDVIYRQLESRIKGGHSAKEHLGEVVGAVSGLAGWEGIADVVLVDQS
                     PIGRTPRSNPVTYVKAFDELRALYASTPLARERGYTPSTFSFNVAGGRCEACEGAGHV
                     QIEMVFLADVFVPCEICGGRRYKREILDVKVDGRSIHETLQLTVDQALSIFHRQGGLA
                     RTLWHLQQVGLGYLRLGQPATTLSGGEAQRLKIARELSSAGGRSRRGRKFYILDEPTT
                     GLHLDDVRTLCRVLDRLVDAGHTVLVIEHHLDVIKRADWVIDLGPGAGADGGRVVAEG
                     DPEAIAQARGSVTGRYLRGGS"
     gene            44659..45531
                     /locus_tag="VFU75_07095"
     CDS             44659..45531
                     /locus_tag="VFU75_07095"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEU5466640.1"
                     /translation="MTRRQADLLLVFTCAIWGLSFPTVKVALADASPLAFVAIRFTLG
                     ALILLPGTRLRPLPSRGEWFAGLLLGALLGVGFATQAVGLAYTTASRSAFIVALSSVL
                     APLVAFVAVRERLSWWTVAALGLATAGIYFLTDPGAGGLNRGDWWTLITALVFGAQIV
                     AVHELGLRFDIRRLVFIEIVVVAVGVGVAAPLLEPVRFAVATQSLLALAYCGVAATAV
                     ALLLQMRAQKDLSSGRAALLFCTEPVFASLASWLWLGERLSLTQWVGGGMIVVGMLVA
                     EPKEAVSHQPSAVS"
     gene            45603..45675
                     /locus_tag="VFU75_07100"
     tRNA            45603..45675
                     /locus_tag="VFU75_07100"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45636..45638,aa:Asn,seq:gtt)
     gene            45772..48672
                     /locus_tag="VFU75_07105"
     CDS             45772..48672
                     /locus_tag="VFU75_07105"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HEU5466641.1"
                     /translation="MPRKPKARPPRPPHPPHPPAPPEGDLREAVSILTATLESTADGI
                     LVVNRTGAITTYNQRFLEMWRIPPAVAQSRNDDQLIGFVLDQLQDPNVFLAKVMELYG
                     RPEAESHDAILFKDGRVFDRFSVPQRVGTKVIGRVWSFRDVSEVRRATEELRRNEERF
                     RALVENSSDVILLLRPDGTIHFSTAAAFPILGYEAGENIGKSIFDLIHPEDLPRARGV
                     FGELMRRPGSKVYLECRARRKDGDWGNLEAVGVNRLDDPSVEAIIVTTRDISDRKRAE
                     RVQQATYRIADAASIERSLEDLLRAIHSIVGELMPADNFYVALLEPDGESVTFPYFVD
                     RFDAQFEPLPLGRNLTSYVIRSGLPLLATSDVFEEMVARGDVDEVGAPSIDWLGVPLT
                     LENRTIGVVTVQTYSEGVRLTEADKDILTFVSAQIARAIERRRAQDALRQAEERYRAF
                     VTQSTEAIWRVELDHPIPVDLPEEEQVNRLYRDAYLAECNEAMVRMYGFESVDQAIGA
                     RLGNFIIQEHAWNRETWGTFVRSGYRLSDIESFERDRSGAHKIFLNNRIGIVAGGHLR
                     RIWGTQRDVTAQHHLEDQLRQAQKMEAVGRLAGGIAHDFNNILTAILGTCSLMQRDLP
                     AGSRSREDVDEIQRSAARAAGLTRQLLAYSRRQVLQPEVLDLNVVVSEMDKMLQRLIG
                     EDVELVTILGEDLGRVMADPGQIEQVLLNLAVNARDAMPQGGRLTIRTANRRDTPVPG
                     ASGQPVSRVMLAITDTGIGMDDRVRSHLFEPFFTTKEVGKGTGLGLATVYGIVRQSGG
                     SISVESAPGKGSTFSVFLPRVEDASFRRASLPAAIPADSGEKHAPSAHILLAEDEPRV
                     RLLTQRVLEARGYRVLAATDGQVALELASDPALKLDLLVTDVVMPGMSGPDLARRLRE
                     SRPGLPVVYMSGYADQGLAHEAAATGGAFLSKPFETETLERLVQSALERRAQ"
     gene            48691..49248
                     /locus_tag="VFU75_07110"
     CDS             48691..49248
                     /locus_tag="VFU75_07110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5466642.1"
                     /translation="MQILIVEDESVTREVLKRLLLAAGHEVTDAADGAEAWELLQSIR
                     IPVVISDWEMPQMEGLDLCRKIRNRSGEYTYFIMLTAKSGRAEYLEAMDGGVDDFLAK
                     PLDAVDLQCRLRVAERILGLRREVQQLEGLLPICSYCKRIRDEMNRWTSIETYIEQRS
                     RAEFSHGICPDCYKKHVEPVLNPGQ"
     gene            49446..50498
                     /locus_tag="VFU75_07115"
     CDS             49446..50498
                     /locus_tag="VFU75_07115"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466643.1"
                     /translation="MPLPPIAGHEALRARFADAIRAGRLPQTLLFEGPRGVGKQRLAL
                     WLAQTLLCETASAPCGKCASCRQVENLSHPDLHWFVPVELTKKGADADKQIDLVEEAI
                     GEVLAERREEPFYAPPAGLAAHSMASARLMLRRLALKPAQGSRAVFILGDAERLAAQK
                     GQDGAANAVLKALEEPPPTAFIILTTSEPDALLPTILSRTVRVRVGRLPDSVVTSFVQ
                     QHMVSRLGDAASVARRVQGAEGAIGRLVATEGSEGSGAAAADRLLQAIRGGTAARYGA
                     ALTQPPFQARGAFTSMLDALLERLRDEAREGGDTSKVMAAMTRVLEARELAQGNVNPQ
                     LLTAVLTEDLAGGGRS"
     gene            50495..50959
                     /gene="moaC"
                     /locus_tag="VFU75_07120"
     CDS             50495..50959
                     /gene="moaC"
                     /locus_tag="VFU75_07120"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HEU5466644.1"
                     /translation="MKLSHLDQEGRARMVDVSGKPATARSAVAEGRIRMSEEAFGLVR
                     DQAVAKGDVLGVAQVAGTMAAKRTAELIPLCHPLALDHIAVEATLDEALPGVQVRASA
                     RMVGRTGVEMEVLTAVSVALLTVYDMVKAVDRHMAIGPVHLVEKHGGTSAEA"
     gene            51020..>51303
                     /locus_tag="VFU75_07125"
     CDS             51020..>51303
                     /locus_tag="VFU75_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466645.1"
                     /translation="MRTTTQRLMLRGTVIVGFALAVSAVGGWVRRATADDVTLPEATV
                     PAADVRGMEQTLDQVQGELTVAKLQLERATAIIGFSSKYGIPADLASNIY"
BASE COUNT         8405 a        17469 c        17178 g         8251 t
ORIGIN      
        1 accagcggcc acatcgcctc gatgatcagg gacgagtcgt cgatcgagcg ggtgcccgcc
       61 agggaattcc cgaagagcgt gtagtcgacc cagatgcgcg cgagccgctg caagttgtcc
      121 gcctggagcg gcatgccctt cacctgactg gcccaaccgg ccagctgttc ggccgagagg
      181 gactggccgg ccgcggacgc ggcatccttg gcatgcgcgg agaacgcgtc acgcagcgcg
      241 ccacaggcga tgagggggag aaccaagagt accgcaccaa aacgacgcat tccgtctcct
      301 agggatgtgg gtcctgcaga accgcggtga gggcccggac cagcccgggg ccgacggctt
      361 cgcctccgag gcgtcgcaac cgcaacgaca ggggctgcgc ccggcgcacg gtggcttcga
      421 actgcaccgc gtcgagcgcc gtgttgaggc ccgccaggcg cggatgggca tctcgccgaa
      481 acgtaagccg cgcctcgtca cccgaaatca ggactgtttc cagccccagc cgggcaccga
      541 gcgcgcggag ctccgcgacc agcagcaacc ggtcggcctc ctcgggcggc gggccgaacc
      601 ggtcgcgcaa ctcgtcgcgg acggctgcaa tctcgcacgg ttgctgggcc cgcgccagcc
      661 gccggtacac gtcgagcttc acggcgtcgt ccgccacgta cgcgtccgac agatgcgccg
      721 cgcggtcgag ggtgacgtcc ggcggcgcga acggcgccgc gccgccgctc ccgccgcctt
      781 tcaagtcgag caccgcctcg gacaaccagc ggaggtaggt gtcgaatccc accgcgtgcg
      841 cgtgccccga ctgatccttg ccgaggagat tccccgcgcc ccggatttcg agatcccgga
      901 gcgcgatgcg gtaccccgag cccagatcgg tgtggtgctc gagcacgcgc aaccgctctt
      961 cggcacgcgc gtccatcagg tcgggcacga gcaggtagca gtacgcgcgc cgatggctcc
     1021 ggcctacgcg cccgcggagc tggtacagct gcgcgagtcc gaactggtcg gcccggtcca
     1081 cgatcatcgt gttggcgttc ggtacatcca atcccgactc cacgatcatg gtcgacacca
     1141 gcacgtcgac ttccccggtc gcgaacttcg ccatcgccgc ttcgagctcg cgctcgggca
     1201 tctgcccgtg cgccaccgcc agccgggccc gcggcgcgag ccgcgcgacg cgcgcgccga
     1261 tggtggcgat ggtctccacc cggttgtgca ccacgtacac ctggcccccg cggtccagct
     1321 cccgcgcgat cgcttcctcg agcagggcgt catcccaggg ctcgaggaac gtgagcaccg
     1381 gggaccggtc tttggggggc gtctcgagca gcgtgaggtc ccgcagaccc gccagcgaga
     1441 gatgcagcgt tcgggggatc ggtgtagcgg tgagcgtcag aacgtccacc gccagcttca
     1501 acgccttcag ccgttccttg tgtcgtaccc caaagcggtg ctcttcgtcc acgaccagga
     1561 gccccaggtc gtggaagagc acgtccttgg acaggagccg gtgagtgccg atgacgacgt
     1621 cgacttcgcc cgcggcggct ttcacgagcg cggcggccgt ttccggcgca ctgcggaacc
     1681 gggaaagcac ttcgatccgg acgggatagt cggcgagccg ttcgcggaag gtgcgtccgt
     1741 gctgctccgc gaggatcgtg gtcgggacca gcacggcgac ctgcttcccc gcctgcacca
     1801 ccttgaaggc ggcgcggagc gccacctcgg tcttcccgta gcccacgtcc cccacgagga
     1861 ggcgatccat gggccgggcc cgctcgaggt cccgcttgac ctcctcggtc gccttccgct
     1921 ggtcaggcgt atcctcatag aggaaggcgg actcgagctc gcgctgccag ggcgtgtcgg
     1981 gcgggaaggc atacccgtgg gcgagctgcc gccgggcgta cagatccagg agctccgagg
     2041 ccatggccat gatggccgcg cgggtcttgt cccgctgccg ctgccaggtg gtggcaccca
     2101 gacggtgcaa gcgcggcggc ggggcgtccg gatccccgct ggtgcggtag ggctcgagca
     2161 ggtcgatccg gtacagcggg acgttgagac ggtccccgcc ctcgtattcc accgtggcga
     2221 catccagcgt cgcctcgccg acctggatgg tctcgagacc gcggaagatg ccgatgccgt
     2281 gatccaggtg cacgacgtat tcaccggcgg tgaacgcgcc gggtcccagc gcgttcaccg
     2341 ccgcccgata gcgccgcgcc cgccgcaagc ggcgtgcgcg gcggaagatc tcgtgatcgg
     2401 tgaggacgcg aagcccggga aagacgaagc ccccgtcgag cgcacccacg gccagggtgg
     2461 cacgcccgga atcgagaagc tcctccagcc gctccagctg gccctcgttg tcacacagga
     2521 tgacggtgcg cgtcgggccg gcgatgagct tgcggagata cttgagatcg cggtcgatca
     2581 gctcgggtaa ggagatcgga aagcgaaccg tcgcatcggg cgcctccgga gcgagccggg
     2641 cgaatccgcc gagcagcgac tcccagcggt tgggcgagag gaagagcgcc tcacgcgatt
     2701 cgggctcttc ccccaggcgg cgcgacacct cgaggtggtg cgccgcctcg gtccacgtcc
     2761 ggtcgacttc ctggcgcagg ggggactcgc ggtcgaccac caggagcgcg tcggagggga
     2821 gcaactcgag caacgaggag cgcacggaat cctgggcacc ggaccgcccc tgctccttga
     2881 gcggcaggat ggtgacccgt tcgacggggt ctccggagcg ctgagtgtcg agatcgaagc
     2941 cccgcaacgt ggcgacttcg tcgccccacc actcgatgcg gcccggcgcc gccatgccga
     3001 agccgtacac gtcgagaatc ccgccccgca cgctgaactg ccccacctcc gttacggtcg
     3061 gcactttcgt gtaccccatc cccaccagtc gctcggccac gtccccgagc gtcgggccac
     3121 ccgcctcgga caaggtcagg cgcgcatccg cgagcgctcc gggcacgccc gtccgctcca
     3181 cggtggcgcg cgcagtggtg atgaggatcc tgatgcggcc ctcgagcagg gcgacgaccg
     3241 tctcgatgcg ctcaccggcg atttcgacgt gctgctcttc gccgccgagg gattcgcgct
     3301 gtggatagag cgcgacggcc ccgggggcca gcgcctcgag atccgaaagc cagcgctccg
     3361 cgtcggcggg tgtaggcgcc acgatggtga gcacgcgctg ggggaagcgg cgcgcgaggg
     3421 tcgccaccag cacggccgca gaggatccgg gcagaccgcc gatacccatt cgctgacccg
     3481 cgcgtgggag cgtcgccgcc agcgcgtcga acgcggggag ctcgctcatg cggtccaaca
     3541 ggaagtcgag gggcatcagc gcgtcgtcac ggcgacgatc cactctccgg ccacgagcag
     3601 caatgccagc atcagcagca agccacctgc atctcccctc gcgctcccgg taaaggctgc
     3661 atcccggaat gccgacgacg aaaacgactc ggcgtccagg gccctcgaga acatggatgg
     3721 cgtcgcgggc gtcaggtccg actcccgcga gtctggcccg agcaccagcg ctccgacggt
     3781 gtccctgcca ttggtccgga actgcatgcc cggcgccccg gtggtatcgc tgaccggggc
     3841 ctcgccctgt gccaggacgt tgacgagcgc gtccatgaag ggcacgaagg agggcgtggc
     3901 gggcaacgtc gtccaggcgg tgtccagtgc gcttccgatg agcagggtcg atccttcccg
     3961 caccagccac ggtgagccat tcaccgtcgc cagcacggtg gcggggtccc cgccggtcag
     4021 ttcgagccgc cggccgaccg gcacccctcc gagcccgctt acgccagagc cgcgaaccga
     4081 gacgcggccg ggcgtcgcgg gcactccgaa gcgccagtgt cctcctcgaa gtccgagcgc
     4141 ccggttgacg gcaccgacgg atgcgggatc cccggtcgcc ggaacgaccg cgaatcgccc
     4201 cgtcgggagc gtcgcgatca cgacatcctt gcccgcagcg atgcggtctg cgttgagcaa
     4261 cacgtcgagc gcggcagcga cgaagggacc cgcggtcggc gccgcgctca cccgcgcggg
     4321 cgggtgtacg cgttggacga acaggcggcg gtcgtccccc cgcagctcgt cagcctccag
     4381 gtccacggat ccggtccacc acccgacggg cgcggcgggc aacgcgatcg cgagtgtgtc
     4441 gcccggcttg gcgagcccgc gcgaaatcac ccggtcgcga taggtcacgg tgagcgttcc
     4501 cgactttcca cccggcgtcc ccacgatggg aatgagcagc ctcgtatcca ccaggcgcag
     4561 gtcgccgacg ccgcggttcg gcgcgacgcc gctcctgggt tcgagggcaa tgacgtgcac
     4621 ggatccggcc gggaccgggg ggccgctcaa cgcgctcgcc tgtccatccg agatcacata
     4681 cacatcgcgg tcggagagcg tcgcgccggc ggccactgcc accgcccgac gcaccgcttc
     4741 cgagagatcc atccggcccg cttcgggccg ggcgtcatca atcgccgcgt ccaactccgc
     4801 gggagtgccg cgccggacca cgccgtcggc cagcagcagc cacaaccggt cgccggagcc
     4861 ggcgcgtgcg gtgaccgcgt gcgccgccgc cgcgagtccg tcgagcaccc ggcggccgtc
     4921 caccaccacg ccggaggaca gcgagttgtc gaggacgatg acggccgccg tcggttcatg
     4981 gcccgtcgcc cgtccccatc cgcgcacctg cggtcgcgcc gcggccatga cgatcgaagc
     5041 gatgagcaaa gtccgcagga ggagcaggag caggtgtcgc agccgcagcc gcctggcggt
     5101 acgccgctcg gcatccgcga gatagcgaac ggccgggaaa tcgaggacgg ggggcgtgcg
     5161 gcgctgccgg agatgcagga gcgccgggat cgccgccgcg gcgagcccga agagggccag
     5221 aggatgaagg aagatcacgc gagccggcgg agtgcgacgc cgaaaggctg atcggtcgtg
     5281 acgtggtggt aggcgatctc gtgccggcgg caggcggacc gccaggccgc gatttcccgg
     5341 cgcaccgttt cgcggtagct gcgagccagc tcccgggggc ggacgaccac cgaacccctg
     5401 gactcgagat cctgataccg gacttccggc ggacccgcga gatcgacctc cgcgggatcc
     5461 atcaggtgca tcaccgtgac ctggtgacca cgatgcctga ggtaccgcag ggcggtcaac
     5521 gcgagatcgc gatcgaagag gaggtcggaa atcaggacca ccatcccccg gcgtgcgagc
     5581 cgcgcggtca cctgccgcag ggcctcttcg gcggcggtcc cctcaccgtc gggagtctcg
     5641 atgagcccgc gcacgatgcg gtgccattgc ccgcttttca ctcgcggtgg aagagcctgc
     5701 cgaatcctgt cgtcgaacgt gatcaagccg gtcgcatcgc gctgccggag caacaggagt
     5761 gagagcgcgg cggcaagccg gtccgcgtac gcacgcttgg tcagccggtc gggtgatccc
     5821 gtccacgcca tggaccggct gacgtccacg agaatcatcg cgcggagatt ggtctcctcc
     5881 tcgaactgct tgacgtaggg ccggtcggtc cggcccagaa tgcgccaatc caggtatcgg
     5941 agttcgtccc ccggctggta cgcccgatgc tccgtgaatt cgacggagaa cccgcgaaag
     6001 ggggagcgat ggaggcccgc aagaaatccc tcgaccacac cggcagccgt gatctcgagg
     6061 cctcccagct gggcgacctc acgggggtcg agcaaatcga ggcgggatcc ggttgccatt
     6121 cggggcggaa gctaacggac gcccgagcgt ccgggccagg agacacccgg gtgcctgaca
     6181 gctttcctgt taggtctcaa gtcgttattg aacattgtgt tacaagtacc tgacgcgaac
     6241 ctgtcggctg gcatgttctc tgcacccaag gggctcccgg gagactcatg ctcattcacc
     6301 acgccatcat caccgctctc gcgctcgctc cgagcccggc gcacccggca gcccccagag
     6361 gacccgggca cgacgggaaa ggccgcgtcc aggtgtggac ggagaacggg aacgacccgt
     6421 accggacggg tgaccaggcg cgcgtgttca tccgcggcga agaggacgcc tacatcacgg
     6481 tgttccgggt ggacacggac ggccggattc aggtgctctt cccgcgcgcg ccgtggatgg
     6541 acaactacgt ccggggcgat cacgagtacg agatcgagca gagccggagc tcgaatgcgt
     6601 tcgccgtcga cgacgacccc ggggtcggct acgtgttcgc ggtctcctcc cccgatccct
     6661 tcacgttcga tccggtcacc gacggcgacc actgggatta ccgcgccatg gacggcggcc
     6721 ggatccaggg tgacccgtac gtcgcgctga ccgatctggc cggacgcatt ctcccgtcca
     6781 acgacgaaga ctgggactac gacatctccc catactacgt cgagcgtcac tacgactatc
     6841 cccgcttcct ttgttatgac tgtcacgcgt tcgcgagctt tggctcctgg gatccgtacg
     6901 ccgccacgtg tccgcgtttc cggatcgtgg cctacgacga ccccgtgtac tacccgtatc
     6961 actactatca gggccgcgcg gtggtagtgg cgcggccatt gcgccccgag ccgcggttcg
     7021 tcttcaagga ccgggacggc gcccccgatg agcagttcgt cacggtaacg cgggaccgat
     7081 cgagagagga gggcagaaat cggggagcat cgggacggga cttcggcggg cctgggcgcc
     7141 tgccggcacc cagccggaca ggtcgggagt acgagcctca acagccggac gcccaacatc
     7201 ccgatcaccg gcaacccgat cagcgcaggc ggccgccgga gcaacggccg gcgcaacccc
     7261 ctccgccgcg tcagcagccc aagccgaacg ccgaaccgcg ccggggaccg cctccacgcc
     7321 cggcaccggc ggcgaagtca ccaccgccga aggagcagcc accgaagaat cagccggagc
     7381 tgaggcgccg gaagccgaac taacgcacgc ccgccagcag ctccgcgttg gtgggcgcgc
     7441 gcttcatcaa ggagagcagt tgctgtatcc cctgctccgg cggcaggtcc acgagacccc
     7501 gccggagctt ctgtttcgcc tcgacggtgg cggcatcgcc aagcagctcc tcgcggcggg
     7561 tacctgacgc cgcgacatcg acggccgggt agatccggcg gtcggcgagc tcccgcgaga
     7621 gtctgagctc cgcgttgccg gtccccttga actcctcgaa gatcacatca tccatcctgc
     7681 tgtccgtctc gaccaatgcg gtggcaatca ccgtaagtga cccaccctgg ggaacggccc
     7741 gtgcggcgcc gaagagcgcc ttcggcttgg ccagagcgtt ggcgtcgagt ccgcccgaca
     7801 tcgtacgccc gctcccccgt cccccggccg tgttgtgcga gcgggccagc cgcgtgagcg
     7861 aatccagcac gatgaccacg tcccggccga gctccacctg gcgccgggca tgctcgaaca
     7921 ccatgtccgc gacggcgacg tgacgttcgt gaggaagatc gaagctcgag gcgaccactt
     7981 cgccccgccc ccagtcgacc atctcgctca cctcttcagg ccgctcgtcg accaggagga
     8041 tcatcagcgt cgcgccggga tagttgatgg ccactgcttc ggcgaccgcc tggagggtga
     8101 cggtctttcc ggccttcggc ggagagacga tcaacgcgcg ctggccgaat cccagcgggc
     8161 agacgagatc aacgacccgc tgaatcgcgg ccgttctggg gctcgaggcc ttgtcggtct
     8221 cgaggcgcag ggcccgctcg ggatgcacgg cgccgagctt cgcgaattcg ggccggtcgg
     8281 cgagccccgc gggaggggcg ccattgacgc tggtgacgcg accgccttcg agcaccacga
     8341 gatcaccccg gcgcagtccc ggcaggcgca cgggcggatc gccgttcgcg ggcaggtacc
     8401 cgttggccgc gagccgcagg gcaccgccct tgggggtggg gtcgtaccag ccggtgatgg
     8461 ggggcattcg aagatcgaaa gctaaggggt caggggccgg ggatcagggg tcagtcgatc
     8521 atgtctggac cccagacccc caacccccgg cccctctata ttccccgcca atgcgtcagt
     8581 ccctgatccg caacttctgc atcgtcgccc acatcgacca cggcaagtcg accctggccg
     8641 accggctcat cgagcacacg ggcaccctcg acaaacggct catgcgggcg caggtgctgg
     8701 acacgatgga cctggagcgc gagcgcggca tcaccatcaa gctcaacgcc gtccgcatga
     8761 attaccacgc ccaggacggc gagatctacg agctcaacct catcgacacc cccggacacg
     8821 tcgacttcac ctacgaggtg tcccgttccc tggccgcctg cgagggcgcg atcctcgtgg
     8881 tcgacgcgtc ccagggcgtg caggcccaga cactctcgaa cctgttcctc gcgctcgacg
     8941 cggggctcga gatcatcccc gtcctgaaca agatcgacct ccccggtgcc gaaccagacc
     9001 gccgggccgc ggagctgcac gacctgatcg gatcccgccc cgaggagatc ctcaggatct
     9061 cggcgaagga gggtacgggg gttccggagc tgctcgaagc cgtcgtcaag cgcatcccgg
     9121 ctcccaaggg cgatccggcc gctcccctgc gcgcgctgat tttcgattcg tactacgacc
     9181 gtttccgcgg cgcgattccc agcattcgcg tcgtcgacgg cgtgctgaag gcggggatgc
     9241 agatcgtctt cggcgcgcac aaggccgatc actatcaggt ggacgaggtc ggcaatctcc
     9301 agctggggca caagccggtg gaacaactgg cggccggcga agtcggctac gtggtcgcga
     9361 acgtgcgtgg cgtgcaggaa acgcggccgg gggacaccat cctcgacgtc gagcattccg
     9421 acgttcccct gctccccggg taccgggata tccagtcgat ggtgttcgcg gggctctatc
     9481 cgacgaacgc cgagcagtac gaggatcttc gggacgccct ggagaagctc cagctcaacg
     9541 acgcttccct ccattatgag cccgagtctt ccgtcgccct cggattcgga ttccgctgcg
     9601 gattcctggg cctcctgcat atggagatcg tccgcgagcg gctggaacgg gaattcaacc
     9661 tcgatctcat ctcgacggtc ccgaacgtgg agtaccacgt ctacaagacc gacggtgtga
     9721 aggagctggt cgagaacccg tcgctcatgc cgcacggatc gacggtggac cgcatcgagg
     9781 agccgtacgt caaagcccgc gtcctcgccc cctccgaata catcggcgcc atgatgaagc
     9841 tcggccagga gcgccgcggc gtgtacctcg ggatgcacta cgtggatccg acgcgggtgg
     9901 agttcagctg ggagtttccg ctcgccgaaa tcatcctcga cttctatgac aagctgaaga
     9961 cgatctcgcg cgggtacgcg tccctcgact atgaattcct ggcctaccgc cgctcggagc
    10021 tcgtgaagct cgacatgctc ctgaacggag acgcggtcga cgcgttctcc gtgatcatcc
    10081 atcaggacaa ggcgtatgag tggggccgga agatcgcgga gaaactgaag gagctgatcc
    10141 cccggcagct gttcgaggtg gtgattcagg ccgcgatcgg cacgaaggtg atcgcccggg
    10201 agtcgatccg tcccctgcgg aagaacgtga ccgccaagtg ctacggcggc gacgtcacgc
    10261 ggaagcggaa gctcctcgag aaacagaagg aaggaaagaa gcggatgaag caggtcggca
    10321 cggtcgagat tccacaggaa gccttcctgg cggtgctgca ggttgactag cgccgtcgtg
    10381 gtgcagacgg cgtttctggg agacgtggtg ctgacgacgc ccctgctccg gacgctcgcc
    10441 gtccggcatg gcccggtgga cgtcgtcacc acgcccgcgg ccgctcccct cctcgacacg
    10501 catcccgcgg tccggcaggt gatccggtac gacaaacgcg gtgccgaccg cgggtggggc
    10561 ggtctcaccg ccctcgcgcg ccgcctcagg gaagcgcact atgaagtggc ctatgtgccc
    10621 caccggtccc ttcgcagcgc gctgctggcg ctgtgggccc gggtgccgcg gcgggtgggc
    10681 ttccgggacg gctggcggtc gctgtatacg gagagcaggg cgcgtccggc gggcggacac
    10741 gaagtggacc gtctgctggc gctggcgggg gaccccacgg cgatccgcac gccgagcatc
    10801 gggctgacac cggcggacca cggggcggcg gcggccttcg tggaggccga ggcgttgggg
    10861 agcgacgtgg tggcgctcgc cccgggatcc atttggggca cgaagcgctg gccgcacttc
    10921 actgcgctcg cgggtcgcct ggccgggagc cacgccatcg ccgtcgtggg aggtcccgga
    10981 gacggcgagc tcgcggccgc gatcgtggat cacgtacgat cggccggggg acgggcggcg
    11041 agcgggtgcg gcaagctgac gttgcgggaa tcggcggcgc tcttggcccg gtgccgattg
    11101 ctcgtgacca acgacagtgc acccttgcat ttcgcgcagg ccgttgggac gccgaccgtc
    11161 gccgtcttcg gtcccacgct gccggcgttc ggcttcggtc cgttgcatcc gggggaccgc
    11221 gtcgccgaag tgacggacct gacgtgccgc ccctgctcca agcacggacc ggccgcgtgt
    11281 ccgctgggcc atcatcgttg cctgcgggat cttcccgtcg aacgggtgat cgccgcaatc
    11341 gaggagactg gtgcgctacg tcgtcgggat tgatatcggg gggaccaacc tggtcgtcgg
    11401 cacgattgcc gaggacgggt cggagctgct cggtgaggtc acggagccga cgcactccga
    11461 ggagggcgcc gacgccgtca tcgctcgcat cggccgtctg gtcaagcagt cgacggccgc
    11521 cgccaagggc aaagagatcg tgggcgtcgg catcggttcc cccggtcctc tcgacactca
    11581 ccggggcatc gtcctgttca ctcccaacct cgggtggacg aacttcccgc tccgggatcg
    11641 agtcaccaag gtcaccggcc tgcccgcgga gctggacaac gacgccaatt gcgccatcta
    11701 cggcgagtgg tggcgcggag ccgcgcgggg cgcgaaggcg gtggtggggc tcacgatcgg
    11761 aaccggcatc ggcggcggga tcgtgctcga cggcaagatc taccacggcg tgtccgatgt
    11821 ggcgggagag atcggccaca cgaccatcga cgcgaacggc cgccgctgca agtgcgggaa
    11881 ctacggctgc ctcgaggcct acgcgtcggg tcccgcgatc gccgcccgcg ccgccgaagg
    11941 catcgagtca ggaatcgaca cgaagctcgg caagtacgtg aacggcgatc tgtcccggct
    12001 gacggcgcag accgtatacc aggcggcgca cgacggcgac cagtacgcgc tcgacgtcgt
    12061 gaacgacacc gccaagctcc tgggcgccgg cgtcgccaac atgatcaaca tcttcaaccc
    12121 ggacgtggtc gtgatctgcg gcggggtgac cctcgccggg gaccggctct tcgaccccct
    12181 ccggcgcgag gtgcaccggc gcgcgttcaa ggatgccgtg aaggcctgcc ggatcgttcc
    12241 cggcgagctc ccgggcaccg ccggcgtgtt cggcgccgcg gccaccttca tcgagaagca
    12301 ccggggcggt cgcgcatgaa gcgcatcggc gtcgtcggcg cgttggtctg ggacacgatc
    12361 tacggccgcg accccgccca accagcggtc gaggaatggg ggggcatcgc gtacgcgctg
    12421 gcgggtctgg acgcggcact ggacgacggc tgggagatcg tgcccctgat caaggtggga
    12481 cgggacctcg ccgagccggc cgggcggttc ttgcgaagcc tgcgtcacgt cgcccccggc
    12541 gcccgcttcg tgctggtgcc ggaggccaac aaccgggtga ccctccggta cgagacgaaa
    12601 gagcgccggt gcgagctgat gtcgggcggg gtgccgggat ggacgtgggg tgagctgggg
    12661 ccgatggtcg gtgatctgga cgccctctac gtcaacttca tctccgggtt cgaaatgagc
    12721 ctcgaaaccg cccggatgct gcgccgggga ttctcgaagt tcatctacgc cgacctccac
    12781 agcctgttcc tggccaagga cgcgaacggc ctcagaacct tgcaacccct cgtggatgga
    12841 cccggctggt tcggatgctt cgacgcggtt cagctcaatg aggaagagat gggccagctc
    12901 ggtcccgatc ccctggcggt cgcggccggc gcactcgccg ccggcaccgg tgtgctctgc
    12961 gtgacgctcg gccgccgggg cgccgcgtat ttcaccggct caccggtgcg gacggcgcgg
    13021 gtctcgagcg gagagacggg cgcggcgcca ttcgaagacg gcgatcccac cgggtgcggc
    13081 gacgttttcg gtgcgaccgc ggccgcggcg ctcgtccagg gtgccgacgt cgaagcggcc
    13141 ttgcagctcg ccgcgcgccg ggccgcccgc aacgtctcgt tccgcggggc caccggactg
    13201 cgcgatcacc tgctgggaaa gctggcgggc gtatgacgcg tctggtggac gttccccagc
    13261 agttcgacga ccgctccttc gaacagttcg cctcgtcgtt tgcgcagggt gcggcgggcg
    13321 gtgagcggct gttgttcgac gcgcacgcca ccgagtgggc ttcaccctac ggcttcgtgg
    13381 gactgctcgt ggccggtcag gccgtgcaga gcgcggggcg cgagcgtccg ctcctgacca
    13441 tacccgccaa tccccgggtc ttgagctatt ggacccgcgc cgggttcttt cagcaagccg
    13501 cgacgctgtt cgagattcac ggcaaggtgc cgaagctgaa gacgtcggag gagagtgacc
    13561 tgctccttcc ggtcaccgcc gtgcgtgcgg ccgaggacgt gcaccaggtc gtcagccaca
    13621 ttcagcaacg ggccaccgcg atcctcacgt cgcaattggg catcgacccc aaggcgaccg
    13681 tgggatttgc tatggcgctt tcggaggcgt gccagaatat tgtagaacac gcgggcaccg
    13741 gcggctgggt cgcggtgcag cgctatgaat ggaagcggcg gctgtccaag aaggtcgtgg
    13801 tcatcgccgt gggagacgcc ggcgtcggat tccggcacac gctggagccg acgcaagcca
    13861 agcgattcgg agaccggtgg ggtgatgggg cggcgttgga agcggcgctg atccagggca
    13921 ccagccggtt ccgcgacccc ggccgcggtc agggcctgaa ggggatccgg agctacctga
    13981 gtcgttggga cggcaaaatc gccatccgga gcggcaccgc ccgtatcgcc atcgcgccct
    14041 cgtgggacga cgacgcgccg ctcaccgaag gcctgagcgc gtttccaggc tcgcaagtgc
    14101 tgttgatcat tcctgagcag gggaccggca catcgtgatt gctccgatcg acgtacacgc
    14161 cgtgttgcag gaatccgtgc ccggccccta cgccgacctg gtcacccgcc acaccgggcg
    14221 ggccgtgcgc gagcggatcg agcgcgccat cgccgacgcc accgtggcgt ccatggactt
    14281 caccggcgtc cgctgcatcg actactcctg cgccgacgaa atcgtcgcca agctcctccg
    14341 caccgtgcag atcctcctgc tccagggcat caccgaagcg caccggctcg ccatcgagcc
    14401 cgtgctgcag gggcacggcc tggcggtggt gatccagacc gggacgctgc ttgaagtgct
    14461 gggccccccc gacgccgccg aggtgatatg cgagcggctc ctcgagagcc ggctggcgga
    14521 gcgcaccgct ggaggcaccc tcgcgttgac ggccgcgtga gccgccgccg gctgggtctt
    14581 tcgacctccg cgatccacgg cgctcccgag ccgcgtcccg actggtcgcc ggtgaccacc
    14641 ccgatctatc agagcagcac cttcaccaat ccgatcggct cgacccagga agtcctgtac
    14701 acgcggtacg gcaacaaccc caaccagatc gccatcgcca aacggctcgc gctgctcgaa
    14761 ggcgccgagg cggcgatatt cgtcggctcc ggcatgggag ccgccgccct cgcccacctg
    14821 gcgattctcc gccccggtga tcacctgctc tcctccgagt ggatctacgg cggcgtgtac
    14881 cggctcttca cccaggagtt cggacggctc gggatcgaag tctccttcgt caacccggtc
    14941 gagtcccgct cctggaagcg tgcgctgaag aagaacaccc gcgccgtctt cgtggagaca
    15001 ccgaccaatc cggtcatgcg cgtcctggac atcgattcga tcggcacact ctgcaaagcc
    15061 gaggggctcg ccctgctggt cgactcgacc ttcgcctctc cctacaactt ccgtccgctc
    15121 gagcatggcg ccgacgtggt gattcacagc gccacgaagt acctgaacgg gcactcggat
    15181 gtgatcgccg gcgccatcgc cggtaccgag gcggtggtcg aggaggtgcg gaagctgatg
    15241 tgggtctacg gacaggcgct cgaccccatg gcggcctggc tcatcgagcg cggtctcaag
    15301 acactctcgg tccggatgga gcggcacaac aggaacggac agcaggtcgc cgagtggtgc
    15361 gcgaagcaca aggcgatcgc ggcggtccac tatcccggcc tgcccacaca ccccgaccat
    15421 cagtcggccc aagccacgat gcaggggttc ggcgggatgc tggggatcga gctcaagggc
    15481 ggcgtgcgcg ccgccgagcg cttcctgcgc ggcctcacca tcgcggcgca cgcgccgtcc
    15541 ctgggtggcg tcgagaccct ggtctcggag ccgcggctca cgtcacacgc gtccctcacc
    15601 gcggacgaac gcgccaaggc cggcattccg gacggcttcc tgcgcttctc cctcggcctg
    15661 gaggacgcgg acgatctgat cggcgacttc gcgcagtcac tggcgcaact gtagtgatca
    15721 agttcacgaa cgtcttcaag gcctacccca agggcgggct cgcgctcaag gacgcgacgt
    15781 tccacgtcgc caagggtgag ttcgcgttcc tcacgggtca ctccggcgcc ggcaagtcca
    15841 ccatcctcaa gctcatctac gcggaggagc gcgccaccga gggcgaagtc cgcgtgtcgg
    15901 gcttcaacct cgccgatctg cctccccgcc aggtccccta cctgcggcgc cgcctgggaa
    15961 tcgtgttcca ggatttccgg ctcctcgagg accgcaccgc ggaagagaac gtcggattcg
    16021 ccctgcaagt caccggcgcg agacgggcgg ggatcgaggc ccgggtgatg cgcgtgctca
    16081 cgcaggtggg cctcgcggcc aaggccacgg cgtatccgag ggagctctcg ggcggcgagc
    16141 agcagcgcgt cgcgatcgcc cgggccctcg tgaacgaacc cacggtcctc ctcgccgacg
    16201 agccgaccgg aaacctcgac gagcgcgcca cccgcggcgt gtttcagctg ctgcgcgaca
    16261 tcaatgcgtc ggggacggcc gtcgtgatgg cgacgcacga cctggatctg gtgcggcagg
    16321 cgccgtatcg ggtcatcgag ctccaggacg gcgccctggt atacgactcg gccgtggatg
    16381 aaccccgcgg tgagcagtcg ggaggggcgc catgaacttc gtgatgcggg aagcgatgct
    16441 cacgttccgc cgggcaccgt tgctctcggc cctgtcggtt acgaccatcg ccttttcgct
    16501 cttcgtcgtc ggcctcttcg ctctcgtcgc ggtgaacgtg caacgggcgc tgagccaggt
    16561 ggctgaacgc gccgaggtgg tggcgtatct gctgccggga acgccgatcg agaccatcac
    16621 ccacgcgctc aaggacatcg aggccttccc cgaggtgacg aacgcctcgt acgtctccga
    16681 ggattccgcg ctcgcccggg cccgccgcga gctggtcgag ctccagggtc ccctgcggga
    16741 gctcgaacgg aacccgctcc ccgcgtccat cgaagtgacc ttgaagcccg gggaccgcga
    16801 tgccgaccat gtcggtgccg ttgcggaccg gatccgggac tttgccttcg tcgatgacgt
    16861 ccggtacggg cgggattgga tcgagaagct cgacaagctg cggtcggtgc tggccgcggt
    16921 aggcctcgcc gtggggatcg cgttcgcggc ggtggccgtc atcatcatcg gcgcgacgat
    16981 ccggatgacc gtcctccaac ggggccggga gatctccatc atgcgcctcg tgggtgcgac
    17041 ggactggttc gtccgcttcc ccttccttct tcaggggctc atcaaggggg cggccggcgg
    17101 cctcctggcc gtgggcctgt cctacgcggc ttacctcctc agcaaccggt ggctgatgat
    17161 gcggacggag ttcttcaatc cggagcaggc cgcggcgatc gtggcgggag ggatggtcat
    17221 cgggctgttc ggcagctgga tatcggtagg aaggcagcta cggaaagtct gagaagcgaa
    17281 agacgtcaag caaagacgtc gagcgaaggc gtcaagcaag gacgtgcgcc tctcagttac
    17341 gcctctgctc gacgcacttg cttgacgctt ctgctcgacg ccgttctggt cctggccctc
    17401 ctcagccccc tgtccgcaca gaagccggga agcttcgatc agcaaatgcg cgacaaccag
    17461 cggcgcctgg aggacatccg gcgccagcgg agtgatgccg agaacgagct cgagtccctg
    17521 cggacgcagg tgcactcgat cgaggacgag atcgcgaacc tcgaacggca gaaggaaacc
    17581 accaaccgga tcgtcaacga gatcgaccgg caaatcggcg agctcggcag ccaggtctcg
    17641 cagaccaccg tcgacctgat cctggcccag gacgcgctgg cggaaaagca cgcggtcctc
    17701 gagcgccgga tcgtggacat ctataagcgg gggccgctgt acgactggca ggtcctgctc
    17761 acctccgaga gcttcggcga cctgctctcc cgctacaaat acctctatct cgtcagccgg
    17821 caagatcgcc tcctggccaa cgacatgcag aagctggagg accgcgtcgc ccgcgaccgg
    17881 cacaacctcc tcgacgcccg cgacgcgctc ggccgccgcc gcaacgagcg ggccgacgag
    17941 ctgacgcgct actcggatct cgaagcccgg cggacgcagg acctgcggga gacgcgccgc
    18001 ggcatccggg ccgcccagct caaggtgacc aacagtgaga aggaagagaa ccggctgacc
    18061 gacgtgatcg cgcggctcga gcgggcccgc cgcgacgcgc tggctcgcgg agcggccacc
    18121 gcgaaggcca ccatcaccac cgcgagcctg ggaacactcc cctggcccgt cagcgggaat
    18181 ctgctgtacc gcttcggtcc cgcccccggt cccgacaaca cgcagatcac ctggcacggg
    18241 gtcggcatcg cgtccccggc ggggacgccg gtgcgcgccg tcgccagcgg caccgtggtg
    18301 gacgtgggcg tgcgtggcac ctacctgacc accgtcatcc tgcagcatgg cggcggctac
    18361 tacaccatct acgccaccct ctcggacgcc acggtccacc tcggcgatcc gatcgttgcc
    18421 ggcgaagtga tcggccacgt cggtggcgcg tcgacggacc agggcgccca tctgcacttc
    18481 gagatccggg ggccggatca gatcgcactg gatccgctca actggctgaa aaggcagcaa
    18541 ccataggcat ttcctgaagg gcgttgacgc tggtcttgca cccctgtatt catacaaacg
    18601 tatgaatcat actacgtcta cccccgaccg cctgctcaac gccggctgga agctgttcgc
    18661 gcaacagggc tacgaccgga catcggtccg cgccatcacc cgcgccgcgc gcgccaacct
    18721 cggcgccgtg acctaccact tcggctccaa ggagcacctg taccacgccg tgctggagcg
    18781 gctattccgg gaaatggggg accggctctc gcgcgccgct gccggcaacg gagatcccgg
    18841 cgcgcgcatc cgcgcgatcg tgaggacgat gttcgagttc ttcggagagt acccggatgc
    18901 cccgcggatc gtcctgcagc aggtcacgcg cggcggtgcc gtgccggacg cggtcgcgac
    18961 tcgcctccgc gccaatctcg cgatcattgc gggcgtggtg gccgaggcgc aggcgcgcgg
    19021 tctgtttcgc cccgccgagc cgttgttcgg cgcgttttcg atcatctcac agacggtctg
    19081 gttcgccgtc gtccgccgca ccatccccca ggtcagtcat gccctcggca accccgatct
    19141 cgccgaccgc ttcgagcacc acatcgccga gatcgtcacg cgcggactgg cccggaaccc
    19201 cgagagcatg ccatgaaccg tttccttcgg ctctgtctcg ccggcctggc gccggcggtt
    19261 ctggtcgcct gtcacggcga tgcagcgcag tcccgcatcg cagggaccgt cgagattcgc
    19321 caggtgcggg tgtccccact gacccccggc cggctcgttc ggctgttcaa ggacgaaggg
    19381 gactcggtgc acgccgggga caccatcgcc ctcctggagc aaccggggct tcacgctctc
    19441 atagacgaac gtcgtgcgag ggccgccagc gccgccagcc ggaccggcga agtcggcgcg
    19501 gccatcgccg agagcacgcg cgcggcgaac gacttgatcc gggctgagcc actccgtgcc
    19561 caggggatcg tctcagcgca gcaatacgag gccctgaccg cggccgccgc cgttgcggct
    19621 gcccacctgc agtccgttcg cgccgccccg accgacgagg ccgcggccca ggccgcactg
    19681 cagggcaccg tggcgatccg taacgagctg gtgctgaccg ccccggtgga cggcgtcatc
    19741 ctcacccgct acgccgagcc gggagaggcg gtgtccacgg gagcgccggt cgtgtccatc
    19801 ggcgaggtga cgcgaccctg ggtccgggcc tacttgcccg agcccctcct cgctcggatt
    19861 cggctcggca cccgggtccg ggtccacgcc gacgggtacg cggacagcgc gttcacgggc
    19921 acggtggcgg agatcgcgcc ccaggccgag ttcacgccac gggcggcact gaccgagcgc
    19981 gagcgggccg acctggtatt cggcatcaag gttgaggtcg gaagcgccgg cgggcggctc
    20041 aaggccgggc tgcctgtcac gctggacatt cctctcctgc catgaccgcg gccgtcatca
    20101 cgcggggatt gcggcggacc tacggcgccc tggtggccgt cgatcatctg gacttggaga
    20161 tcgagcccgg ggaggtcttc ggtttgctcg ggcccaacgg ctcgggcaag acgacgacga
    20221 tccgcatgct ttgcggactc gtcgcgccca ccagcggcac ggcggaggtg gccgggatcg
    20281 acgtgacccg ggcccccgag gaggtgcgaa accggatcgg atacatgtcg cagcgattcg
    20341 gcctgtatga ggacctcacg gtggccgaga atctcgactt ctacggcgcg gtgtacggtc
    20401 tgcagggggc ggcgcaggcg gtgcgcgccg gcgaggtggt gcaattcctc ggcctcgacc
    20461 agcgcctggg gcagctcacc ggtcaattgt ccggtgggtg gaagcagcgg gtggcgctgg
    20521 ggtgcgcgct catgcaccgg ccgaccgtgc tgtttctcga cgagccgacg gccggggtgg
    20581 accctgccgc gcggcggtcc ttctgggccg ccatcaagaa catcgcgggg cacgggacga
    20641 cggtcatcgt cacgacccac tacatggacg aggccgagag ctgcggacgg ctcgggatga
    20701 tgagcggcgg ccgcctcatc gcgttgggca cgcccgccga ggtggcccgt caggtgagtg
    20761 gcggcggcac gctggaggac gccttcatcc gtctgcagga gcaggtcgag cgatgatggg
    20821 ccacctccgc cccatgatcc gcaaggaatt catccagatg cggcgggacc ggctcaccct
    20881 cggcatcatg gtggcgattc cggtggtgca gctcctgctt ttcggctacg cgattcagac
    20941 cgacgtccgg cacatcccca cggcggtgtt cgacgactcc cgcacgcccg agagccggga
    21001 catcgtcgcg gcattcgaga acaccgggaa cttcaccatc atcgccaggg tggcgagccg
    21061 tgcggcgctc gatgacgcca tcgcccgggg cagcgcccag gccggcatcg tcgtcccgca
    21121 cgactatccc gaggctctcg ccggaggccg ctccgcgcgg attcaggtca ttgtggacgc
    21181 cgaggatccg ctgtcgtcgc agtccgcact ggcggcggcg tccggcgtgg gtgaagtccg
    21241 gaacctggcc atcctctcgg cggccgcggg gcgcaccatc ctgcccatcg aagtccgggt
    21301 ccgcccccgc tacaacccgg gactgcggag cgcggtcttc atcgtgccgg gtctcgtagg
    21361 ggtcatcctg acgctgacgc tggttctggt gacggcgatg gccatcgtac gcgaacggga
    21421 gcgggggacc ctcgagcagc tcatcgtgac gccggtcacg cgcacggagc tgatgatcgg
    21481 aaagatcgcg ccctacgtcg cggtaggact ggtccagatg acgacggtgt tgtttctcgg
    21541 gcgctgggta ttcgacgtgc cgctcaccgg gagcctgctc ctgctctatg gtgtcacgct
    21601 ggtgtttatc gtcgcatccc tggcgttcgg gcttctgatc tctacgctgg tcaggacgca
    21661 ggcgcaggcg attcaggcga gtttcttctt cgtcctgccc aacatcctgc tgtcgggatt
    21721 catgtttccc cgggccgcca tgccggcggt ggcgcagtgg attggactgg ccctgcccct
    21781 cacgtatttc ctgaagattc tgcgggggat tctgctcaag ggagtgggga tcgagagtct
    21841 ctggcccgag gtggcagccc tcgcggcgtt cggggcggtc gtgatcaccc tggcggtgcg
    21901 gcggttccac aagacgctgg actaggcagc aaactgcgtc gaggaaatgc gtcgagaaga
    21961 aacgtcaagg agaatcgtcg atgcctctcg ttgacgacct tccttgacga tcttcctcga
    22021 cgatcttcct cgacgtcttc ttagcttgta ctgccaatcc ggtcgggcgc ctttttcccg
    22081 accgcgagtg attcctccaa cagccggatc aagtctttcg gctcgaaggc atccggatgc
    22141 gggaacgtca ccagcccggc cgtgaggctc ggcgaccgga cggggccgag atgcggaaag
    22201 gtgtgggcgt cgaccacccg gcggatccgc tccacaaacc cccgggcgcc ggcgagcgcg
    22261 gttcccggaa gaatgagagc gaactcgtca cctccgtagc gcgcgacgaa gtccggcgcc
    22321 cggatctccc gttggagcat ggcgccgaga tcgaagagca cctcatcccc caccgtctgc
    22381 cccgacgtct cgttgatggt ccgcagggcg tcgatatcca gcagtaccag gctgaacgcc
    22441 agggtgtagc ggcgcgcgcg ctcgaactcc tcgcgcagcc gctggtccag ggcatccagg
    22501 gaaccgcacc cggtcagggc gtccgtagtg ccggccgaca cctggcggcg gaacatggcg
    22561 gacctgcgct cttcgttctc gagcacgcgg gccgcggctt gagcgatcgt gttcgcgaag
    22621 tcgacgtcgg tgggatcgag ctccggctct ccccgctcgg tgcgcaagaa gaagactccg
    22681 gcggcgcgtc cattcacgaa cacgggcagc gccgccgagg atcgcacctt cacgtcgatg
    22741 cgctgctctt cccaatgccg ccgaatcgcc gcaaacaacg ggtgctggtg cacgtcgctg
    22801 accaccacgg gggcgcgggt cttcagcgct tcctggatct cggggtagcg gtccagatgc
    22861 actttcaggt cgcgcacctt gggatcctcg tataccgcga ccactcgccc ctgggccccg
    22921 ccgggaccgg tgagcacgaa agagcaatgg gtgagtccaa aagcctctcc cacccgccgc
    22981 accagggtct cgaacacctg ctcgggccgc agggcgcccg agacttcctg gaagatgtcg
    23041 aagagagcct cgcgggtccg caacgcccgc cgcgccgacg acaaggtcgc ccgccggcgc
    23101 cgatcagacg ccagacgcgc ctcgacctcc gtcaaatcga tgggagcccg gatcacgtcg
    23161 gtggcgccgg cgctgagcag ggccgccgta tggagcgcgg tgccgccggt caagaggacg
    23221 acgatcgagg cgccgtcatg gcgaggattc ttgaccatgt cgcgaacccg ctcgatgagc
    23281 gacggatcgt tgggggtggc accgaggaga atgacgtgcg gcgggccgtc ggcagccgtt
    23341 tcggcgtcga ggaccgcgaa cccggcccgg accagcatcc gttcgaggcc ctccggacgt
    23401 gcctcgggag cgccaagcaa caggagtcga gacggtttcg attcagccaa tggactcctg
    23461 gatcgccggg ggacctcgtg ccaaggtaag tttcgcccga tgccgaaaca agctggagcc
    23521 gacggatgag ccggccggca ccgggaaccg ccgggccaag acggcgaatg gtgcgggagc
    23581 agattgcgcg acggggcatc caggacgctc gcgtcctcct ggccatggac cggacgcccc
    23641 gcgagtggtt tgtgcctgac gaatcgtcgg cggacgccta tgccgacgag ccgctcccgc
    23701 tgatcgcggg acagacgatt tcccagccgt acgtcgtcgc gctcatgacg gcgctgctcg
    23761 cccccacccg tcacgaccgg gtgctcgaga tcggcaccgg ttccggctac cagaccgcca
    23821 ttctcgcccg cctcgcggcg gaggtctaca cggtggaacg catccccgag ctgctcgtca
    23881 ccgcggaggc gcgcctgctg cggatgggac tcacgaacat tcagaccctc atcgccgacg
    23941 gcgcgcgggg ctggagcgcc gccgctccct tcgacgggat cctggtcgcc gccgcggctt
    24001 cccaggcgcc gcccccgctc ctcgagcagc tggcgccggg cgcgcgcctc atcatgccca
    24061 tcggcgacca gcgtcggcag gagctcacgg tgttcacgcg actggattca ggggtccggg
    24121 cccacggctt cggcggcgtc cggttcgtcc cgctcgtctc ccccctctca tgaacgacac
    24181 cctcgccctc ctcggcgccc atgtgtccac ggacggcggc gtgtccaccg gaccggcgcg
    24241 cggcgcggaa gtcggcgccc gcgcgattca gcttttcacg aagacgccga atcaatggcg
    24301 cgagccgccc atcattcccg atgaggtcga gcgcttcaag gcggagatgg cgcggaccgg
    24361 catcgaggtg gcggtcgcac acgacagcta cctgatcaat ctcgcgtcac ccgatgacaa
    24421 gctgcgcgcc aagagtctga agtgcttcac aggcgagctc gagcgctgca aatcctacgg
    24481 gattccgtac gtcgtgtctc atccggggaa cttcatggat gaccatgacg ccggactcgc
    24541 gcgcaatgcc gcggcgtacg cggcggcgct cgcggaggtg ccgggtgacg tcggcgtgct
    24601 gatcgagggc acggcaggcg cgggtaccgc gctgggacgc agtttcgaag agctgcggga
    24661 tctccgaaac gggatgccgg aagcggtgcg gcaccgcgtc gggttctgtc tggacacggc
    24721 gcacatgcac gccgcggggt acgacgtcgt cgggaacatc accggcgtcc tggacgagtt
    24781 cgaccgggtg gtgggcctgg agcatctgaa atgcttccac ctcaacgatt ccaaggccgc
    24841 cccggggtcc agattggacc gccacgactg gattggtgag ggaaccatcg gggcggagcc
    24901 gttccgccgc attttgcaag acgcacgttt tgcgaaagtc attaaaatta tcgagacgcc
    24961 caaaggcgac gacccggtgc accatgaccg ccggatgctc gagcgactcc ggtcgtacgc
    25021 ctgaacgaac cccgggcgca gaacgtagtg ggggaccgat gacccgttcg cggacgcgcc
    25081 gcagctggtt gaagccggca ctggccgccc tgctcctgtt ccttcaggtc acaggggcgc
    25141 cggtggtcgg catcgcgcac gcccgcgaac ctggccccgc gccggtctcg ctcgagagcg
    25201 gccactccga tcgctgtcct gtgctccaca acgagctcac ctgcattctc tgtcagtacg
    25261 ccgggtccct cgtcgttacc cggcccgcac agcccgagcc ggacctcggc cgcatagcgt
    25321 ttgcgccagc cgccgtcgtc tcgcggcctc gcgccgtcga acgtccggcg ccctgcacct
    25381 ccccccgagc acctcccgca ctctcctagt ccggacacag gcgcgtggag cgcctgaccc
    25441 agacgcctct gcgtctgggt gctcaggatc gtcctacttg ggagagtggc atggttacgc
    25501 gttcgagttt ctggttgatc gccgtcggca cggcgttggc gattcctgcg catgcgcaag
    25561 gtcccgcgac gccccctgcg ctgacgctca cgcgccggca ggcggtggac accgcgctgg
    25621 cgcacaaccc gcagctcacc gtcgcccgcg agcagctcgc gcaggcccgc gcccaggcga
    25681 ccgagacggc cgcgtttccc gatcccacgc tcaatggcga cctgaacgga caaaggagcg
    25741 tcaccgggtt caagacgaac accggcagtg acatcggtgt gggcctgacg cttcccttcc
    25801 cgaccaagtt cgtgctcctg aacaaggtgg gtaacgccaa tgtcgacgcc gtggcgttca
    25861 actatgatca gctgcggcag cagacggcca gccagaccgc gcaggcgtac gacgcgctca
    25921 tcgtcgccct gcgacaccgg gccgacctgc agctgggtga ctcactcgcc agagacttcc
    25981 tcaagaagac gcaggtccgg ttcgatgccg gcaccgcggc gcgactcgac gttctgaagg
    26041 cgcaggtcga cctcggtcaa gccgagaacg atctgctggc gaaccagcgg gacctggcca
    26101 acgcgcgcgc cgcgctgaat cggctgctcg gccgtccact cggattcggg gtcgaggccg
    26161 cggagtccct gtcggtgccg cccgccctcc cggccgtcga ctctctcatt tcgctggccc
    26221 agctccgccg tccggagctg cagggactgg ccagccagca acgcggcgcc agcgcggcga
    26281 cggccctggc ccagcagtat ttcctgcccg acttgaatct ctcggtcgcc aagaacatga
    26341 cgcagggtac accctcgacg tacacgaccg gcatcggcat ctcggtgccg ctcttcttct
    26401 ggaatcacca gcgaggtcag gtcgcggaat cccggcatca cgaacgggag ctcgcggccg
    26461 cctactccga tctcgaagcg caggtggagc aggacgtgcg cgtgacctac gcgaccgccg
    26521 ccacggcgct ggcccaggcc gtgtacctcc gggatcagct gctgcccgag gcacaccgcg
    26581 cctacgacat cgcgctgacg agctatggcc tcggcggatc gtccgccctc gacgtcctcg
    26641 atgcccgccg gaccctgctc gacgccgaga gccaatacgc cgacgcgctg ggcgccgcca
    26701 atgacgcccg cgccgacctc gaacgggccg tcgcgggtcc ctttgacgct gcttccgctg
    26761 gagttcccaa tgttcactaa ccgcctgacc cgcctgctca cggccgctgg aatggccgcc
    26821 ctcctcgcca ccggctgcgg gcgcggcgcc gcgaaagatc cggaccacgc cggaacgttc
    26881 gggctcacgg agaaccagcg gtcgaagatc catctcgccc cggtggcgga ctcgagcttc
    26941 cacccgtccg tctcggtgtc cggcaccgtc gccttcgacg gggaccacag tacccaggtt
    27001 ctggcgccga tttcgggacc cgtcagccgg attctggtgg agccgggagc ccaggtgacg
    27061 gccggacagc ccctggccta cgtgtcgtcc cccgacttcg ccgccgctct cgcggcatac
    27121 cggaaggcga atgccacggc caccaacctc atccggatcg cgacccggga ttcggtgctc
    27181 ttcgcgaacg acgcgttggc acggcaggat ctcgaacagg cgcagaccga tgcgtcttcg
    27241 gccatcgcgg accgcgatgc cgccctcgac cagcttcgcg ccatcggcgt ggactcggcc
    27301 gggctcgcgg cgatccggga caaccgcaac gcgaccattc cgcagggcgt gattcgcgct
    27361 cccctccccg ggaccgtcgt ggagcggttg atcacgcccg gacagctcct gcaggcgggc
    27421 cagacaccct gtttcacggt ggctggtctc tcgacgatgt gggtgatggg caacgtcttc
    27481 cagaacgatc tgcctgacgt cgcggtcggc gatccggcgg acatcgcgcc gggggccgga
    27541 gcgcgcgtct atcacgggcg ggtggattac gtcggtgccc tcgtggatcc ggatacgcgc
    27601 gccacgtccg tacgcatcct cacgggcaat ccggaccagg gcctcaagaa ggacatgtat
    27661 gtctcggtga ccattcattc ccaccgggcc cgcaagggac tcctggtccc cgtttccgcc
    27721 gtgctccacg acgatcagaa cctgccgttc gtgttcgtgg ccaaggccga cagctcgttc
    27781 gagcgccggc aggtgggact gggcaaccgg atcggcgacc ggtatgaaat cacgtcgggc
    27841 ctttccgcgg gagatgaggt catcgccgag ggcgggctgt tcctgcagtt cgcggaaagc
    27901 caatgagcga gcagctaccg ccacccgcgc acgaggcgcg gacccggtcc gccatcaacc
    27961 ggatcgtttt ctcatccctg cgtcagccgt tcctggtggg gctgttgacg gtgacgttcg
    28021 ccgggatcgg catctggtcc ttttcccgct tgccggtgga cgcctatccc gatctctcgc
    28081 cgacgatggt cgaggtgatc acgcaatggc cggggcacgc gtctgaagag gtcgagcggc
    28141 tgattacggt gccggtcgaa gtcgagatga acggactgcc ccgcatgcag gtcatccgtt
    28201 cgatttccct ctacggcttg tcggacgtca cgatgacgtt tgccgacggg accgacgact
    28261 acttcgcgcg ccagcaggtg ttcgaacgga ttcccgatat cagcctgccc gacgccgtga
    28321 cgccatcggt gtcgcccctc tttgcgccgt cgggcctcgt gtaccgctac gtggtccaaa
    28381 gccccgaccg gtcggccatg gacctgaaga tcatcgacga ctgggtgatc acccggcaac
    28441 tcaaggccgt tcccggagtc gcggacgtct cgccgttggg aggggagacc ttccagtacc
    28501 aaatccttct cgacccgact cgactcgccg gcgcccacct ctccatcagc gacgtggagt
    28561 cggccctcgc ggcgaacaac gggaacgccg gcggcgggtt ctactcggag ggagggcagt
    28621 tctactacat ccgcggagtc ggacggatgg cgactctcga cgacattggg aacaccgtcc
    28681 tcgccaacca caacgggacg ccgatcctgg tgaaagatgt cggccaggtg gtggtgggtc
    28741 atgcgccgcg gctggggcaa ttcggctaca acggccagaa cgacgcagtc gaaggcgtgg
    28801 tcctgatgcg gcgcggcgag caggcgcaga ccgtgctgca aggagtcgag gccaaggtcc
    28861 gcgagctcaa ccagtcggtc ctgcccaagg acgtgaaggt cgtgcccttc tacgaccgtt
    28921 ccgatctggt cgcgttgacc acccacacgg tcgaaggaaa tctggctcgg ggaacgctcc
    28981 tggtcgtcgt ggtcctcgtc ttcttcctct atgacgtgcg ctccggcctc attgccggcg
    29041 tgaccattcc gctggccctg ctgttcgcgt tcctgtgcct cgacctccgg cacatccccg
    29101 ccaaccttct ttccatcggc gcgatcgact tcggcatcct ggtggagagc gccgtggtga
    29161 tggtggagaa catccaccgg cagctcgccc tccgtcacgg aacgtcctac cggatccacg
    29221 aggtgatcgc ggacgcggcg gccgaggtgg accggccgat cttctattcc atggcggtca
    29281 tcatcgcggg gttcctgccc atctacgtcc tgcacggccc gtccggacgc ctcttcacac
    29341 ccatggcgtc cacgacgatt ttcgcattga tcgggtcgtt agcggttgcg ctggccgtct
    29401 tgccggtgct ctgcgcctgg tttctgcgca aaggcgtccg cgaacggcgt aacgcggcct
    29461 tcgagtggct gcgcgagcgc tatacccggg gcctggactg gtgcctggcc cggccccgcg
    29521 ccacggtggc cggcgcggga gtgctgttcg cggcggccgc gctcctgatt ccgggtatcg
    29581 gcgccgagtt catgcctcat ctcgatgaag gggcgatctg ggtgcgggca acgatgccat
    29641 acacgatttc cttcgaggag tcggcgaaga tcgtccccca ggttcgcgcg atcattcgtt
    29701 cctttccgat ggtgacggtg gtgacttcgg agcacggccg gcccgacgac ggaaccgacc
    29761 ccaccgggtt cttcaatgcg gagttctacg tcggcctgaa gccgtatgat gagtggaagg
    29821 gggagatccg gaccaaggca cagctcatcg ctgccatcga ctcccgcctc cgggcgttcc
    29881 ccggcatcat tttcaactac acccagccgg cggaagacgc cgtggacgag gccgagaccg
    29941 gactcaagag cgccttggac gtgaagatct tcggtcccga cctggacacg ctcgagaccc
    30001 tgggccggtc tctcaagaag gcgttggagg acgttccggg gatcacggac ctcacactgg
    30061 tccaggagct ggggcaaccc agcctgacga tcacgcccga tcgcaatcgg atcgcccgct
    30121 acggcctgaa catcgccgac atcaacgcgc tgatcgaggc ggccgttgga ggccaggcgg
    30181 ctacccaggt ggtgcagggc gagcgctcct tcgacctcgt ggtccgcctg gagccccaat
    30241 tccgggagac ctcggaagca attggggaga tccccgtccc cactcccggc gggcagaata
    30301 tcccgctcaa agacctggcc acgattcagg tgaccaatgg cgcgtctttc atttatcggc
    30361 aggacaacgc ccggttcatc ggggtccagt attccgtcga gggccgcagc ctggcgacgg
    30421 cagtgcggga tgcccaggcg cgggcgtccg cccgcgtcac gctccctccc ggctaccggg
    30481 tggtgtgggg cggagagttt caggagtaca ccgcctcccg gcgtcagatg atggtgatcg
    30541 tgcccgtcac cctggggctc atctttctca tcctcttcgc gctgtacagc aacctcaagt
    30601 tcccgttgat cacggtcgcc gctgtgctcc tgtcggcccc agtcggaggg ctcctggcac
    30661 tctggctcac cggcacggcg ttttccgtct cctcgggcat cgggttcctt gccctgttcg
    30721 gcgtgtccgt gcagacggcg gtggtgtaca tctcctatgc caacgagctc cgcggggatg
    30781 gcatgcccat cgcccaggcc acacgcgaag ccgcgctcct gcgtcttcgg cccatcatga
    30841 tgaccgcgct cgtagcggcg ctcggcctcc tccccgccgc gttcgcgacc ggcgtcggca
    30901 cggattcaca gcggccgttc gcgatggtga tcgtgggtgg catgctgtca cagcttgtgg
    30961 taagcgtttt tcttgtgccc gcgctgtact tgctcgtggc cagggaagga gataggctcg
    31021 aggtatgacc atcctggacg tcgagacgtc tagacgtcta gtcttgacgc acagacgcac
    31081 agtggtgagc aggagtcagg tgttcaggac cttgctcctg tgttcctgct tcgcgtcgcc
    31141 agccgcgggg caagtctcct tttccgtatt cgctggcgtc cgacgcagct ccgtactcgc
    31201 ccacgacgtc atcgtgaacg ccttcgacgt ccggccggcg ctggcgcccg cgttttcggt
    31261 caccgcggcg ctgcccctcg acctcccctg gcgggcgtcc gcgggactcg acgtctccac
    31321 cagcgacgtg cgccgggaag acctcggcgg ggccacgcag tcgatcacgc acctgacgac
    31381 ggcagccttg accgtcgggc tctcgcgatc cctcgccccc tgggtgtcgg gatcgatccg
    31441 ggtcggcgcg ctcaagtatc tgccttcgca ggatctcggc ctgtttcagg atggcggacc
    31501 gttcttcccc ttcgcgcaga tcgccttcga cctgtcaccg tccttcgcgt cccgttatgg
    31561 agtcggcctc gagatgaatg gcgacgtcca caagttcatc acggacgccc tgcgggtaga
    31621 gggattctcg gaagcccggc ccgttctcag attggcgctc ggcgtgacct ggacacccgg
    31681 gagtcaccca tgaaacggtg gctcggagtg gtggcggtcg ccctggtcgc gtgcacggtg
    31741 ccggatgtcc ctgagcactc cgggggctac ggattcgact cggtgggaga cgggagcgga
    31801 ctcgttttcc actggacggg ggatcggctg ccggtgcgtt tctgggtgga tccgcgcgga
    31861 aacatgagcg ccctggttca gaacgcgatc gacgtgtggc agaaccagtt catgtacggg
    31921 gagttcaccg gcgtcatcgt gtccgactcc gcacatgcgg acgtgcaaat cgtctgggcg
    31981 gattccgccg cgccaccggc cacgccagat cccggaccac cagtgaaggc ttgtggcgga
    32041 gaaacgcaga acgattcgat ctcgggcggc cgaatctacg gagcgctgca ctccagcatc
    32101 tcgctcctca cactgtcggg agcgacggca gggcaggtcg aagcctgtgt gcgccggacg
    32161 gtgatccatg agatgggcca ctcgttgggc atcctcaatg acaacgcgcc tgactcgacc
    32221 gacatcatgt atttcacgcc cctcgtgccc gtcccgagcg cagctgaccg gacgacgatg
    32281 caggtgctgt accacacggc cccgaccctg cttccgcctg tccgctaggc cacccagccg
    32341 ggtttccttg acaatcaccg gccggtccga tagcataact ccttacccaa aaaggacttg
    32401 aatcctcgat ggccacccgc cgtcaggagg ctctggagta ccacgcacag ggccgccccg
    32461 ggaagattca ggtctctccc accaaaccgt tcaagaacca gcgcgacctc tcccttgcct
    32521 acacgcccgg ggtagcggag ccgtgccggg aaatcgcggc gaagccggag gaagcctaca
    32581 agtacacgat caaaggcaac ctcgtcgccg tcgtgaccaa tggcaccgcc gtcctgggcc
    32641 tcggcaacat cggggctctc gccggcaagc cggtgatgga aggcaagtgc atcctcttca
    32701 aggcgttcgc cgacctcgac gtgttcgacc tcgaggtggg gtccgagaac cccgacgacg
    32761 tgatcaagtt ctgccagctc ctcgagccca ccgtcggcgg catcaacctc gaggacatcc
    32821 gttccccgga ctgcttctat atcgaagaac ggctccgcga gaccctgtcg atccccgtct
    32881 tccatgacga ccagcacggc accgcgatca tctccggagc ggcgctcctc aacgccctcg
    32941 agctcgtggg caaggcgatc ggtgacatca gcgccgtgtt ctgcggcgcc ggcgccgccg
    33001 ccatcgcgac ggccgagcac tacgtccggc tgggcgtaaa gcgggagcgg atcacgctgt
    33061 gcgacaagga cggcgtcatt cacgcggggc gcaccgacct cgacccgtac aagaagaagt
    33121 tcgcgcagcc gatcgacgca cgggatctca gggacgcatt gaagggtgcc gacgtgctgg
    33181 tgggcctgtc cgtcgccggt gccgtgacgg cgacgatgct cgccgggatg gcgccgaagc
    33241 caatcatctt cgcgctggcg aatccgacac ccgagatcat gcccgacgag gcgcgccggg
    33301 cgcgccctga cgccatcatc gccaccggcc ggtcggactt tccgaatcag gtgaacaacg
    33361 tcctgggctt tccgttcatt ttccggggtg cgctggacgt ccgcgccacg acgatcaacg
    33421 aagacatgaa gatggccgcg acccgggcgc tcgccgcgct ggcccgcgaa gaggtccccg
    33481 attcggtgat gcgggcctat gggatcgagc gcctccggtt cggccccgac tatctgattc
    33541 ccaagccctt cgacccccga gtgctgctct gggtggcgcc cgcggtcgcc tgggcggccg
    33601 tgggatcggg cgtggcggga cgggtcatcg acgtcgacga gtaccggcag gagctcgaag
    33661 gccggctcgg ccgggcccgc gaagtcatgc gcggttttgc ggcccatctc caggaagacc
    33721 ctcagcgcat tctcttcccg gagggcgatc atccgaaaat catccgcgcc gcgcgcgtcc
    33781 tcgccgacga gggcagtgcc gtccccgtgc tcttggggga cccggcggag atccggcggg
    33841 ctgccgagga cgccggcgtc acgctcgagg acatcgagat cgtcaatccc gccgaggccg
    33901 ccgattcgga ggggctggcc cgcgcatatt gggaacgccg gcaacgccgc ggcgtaaccc
    33961 tgcgggaggc acgcgcgcgc gtccgtgacc cgctctacca cggactcctg ctcctcaaga
    34021 ccgaccgggc cgacgcgctg gtggtcgggg ccgacatgta ctatcccgac gcggtgcggc
    34081 ccgccctcga agtgattggc gcggcccccg gtcggaagca cgtctcgggc atctacatga
    34141 tggtgctccc ccaggacgtc ttcttcttcg ccgactgcac catgaacatc gatcccgacg
    34201 ccgagacgct cgctgcgatc gcgtccacga cggcggagtt tgtgtggcgc ctcggcatcg
    34261 agccacgtgt ggcgatgctc tcgttttcga acttcggctc ggtcccccat gacggcgtaa
    34321 ccaaggtccg aaacgcggtg gccctgctcc acgaacggga gccgagcctg caggtagatg
    34381 gggagatgca ggctgatacg gctgtcgtaa actcaattct tacaaagact tatccattta
    34441 cgaagttgcg cggcgcggcc aacgtgctga tttttccggg tctggacgca gccaacatcg
    34501 cgtacaaact gctcaatcgc ctggggggcg ctcaggccat cggtcccatt ctggtcggca
    34561 tgtcccgacc ggtacatgtc ttgcaacgcg gggccgacgt cgacgatatc gtgaacctgg
    34621 cggtcctggc cgccgtggac gctcgagaac atcgccgccc cgtgcggcct cacacctaaa
    34681 aggacaccga tggccacgac cgtgaccacg ccccgcaccg gacgccgttc gggcgcttcc
    34741 atcgagtttt ccgggttggc ccccgagcat cacgtgcact ggaactacgg cccggccgag
    34801 ctcgtcgagc acgccatccg ccggaaggaa ggcatgctgg cggacggcgg gcccttctgc
    34861 gccgtcacca gcccccatac cggacgctcc cccaacgaca agttcgtcgt gaaggaagcc
    34921 agctccgagg cgaaggtgtg gtggggaaag gtgaaccagc ccatcgcgcc cgagcacttc
    34981 gaccggttgc acgctgacgt cctccggcac ctgaacagtc aggagatctt cgtccgcgat
    35041 cttttcgcgg gagcggatcc cgattaccgc attccggtcc ggttcgtcac gccgaacgcc
    35101 tggcaggcgc tcttcgtcta caacatgttc ctgcgcccct cccaaccgga gctcgcggcc
    35161 ttcacgcccg agtggcaggt gctgcacgcc ccagaattgc acgcggaccc gaaggtccac
    35221 gggaccaagt cgtcgacgtt catcgtcgtg aacctgggca agcggatcat cctgatcggc
    35281 ggcacgcggt acgcggggga gatgaagaaa tccatcttca ccatcctcaa ttacctgctc
    35341 cccaaccgcg gcgtgctgtc gatgcactgc tcggcgaacg tgggcgaagc gggagatgtc
    35401 gcgctgtttt tcgggctgtc gggcaccggc aagaccacgc tctcggccga tcccgagcgc
    35461 gggctgatcg gcgacgacga gcatggctgg agcgatcggg gcgtcttcaa cttcgaaggt
    35521 ggctgctacg ccaaagtcat ccgcctctcc cgcgagggtg agcccgagat cttccagacc
    35581 acccggatgt tcgggaccgt gctcgagaac gtggtggtgg acccggaaac ccgcaggatc
    35641 gacctcgatt ccgccgccat cacggagaat acccggtcct cctacccgat ccactacatc
    35701 ccgaaccacg tggcgggggg ccagggcggc caccctacgc acatcgtctt cctcacctgc
    35761 gacgcctttg gtgtgatgcc tccgatcgcg cggctcacgc cggcccaggc gatgtaccat
    35821 ttcctgtccg gatacacggc caaggttgcc ggcaccgagc ggggcgtcac cgaaccgaag
    35881 gagaccttct cggcctgctt cggcgcgccg ttcctgcccc tgcatccgaa cacctacgcc
    35941 gagctgttgg gggcgaagat cgccaagcac ggcgtgcagt gttggctggt caacacgggc
    36001 tggacgggtg gcggcttcgg cgtgggccag cggatgcggc tctcgcatac tcgctgcatg
    36061 gtccgcgcgg cgctcgccgg caagctggac aaggtcgcca acgtcaccga gccggctttc
    36121 ggactctcgg tgccgcaaca cgtgcccggc gtccccgacg aggtcctgct cccgcgcaag
    36181 acctggagtg acgcggcggc gtacgatgcg atggccgcca agctggcggg gttgttcaag
    36241 aacaacttcg tccagttcca ggatcaggtc cccgcggcgg ttcgggaggc cggcccgaga
    36301 atttgaccgc gtcttcgatc ggggccccgc tcgaggtcgt ccgcgacccg ctctggaaca
    36361 acatccgcct cgagccggac gccctgacgg tgctggacac gccggcggtg cagcgcctcc
    36421 ggtacgtccg ccagctgggc cacgcgttcc tcgtctatcc cggcgctacg cacagccgct
    36481 tcgagcactg cctgggcgcg tatcatctcg cgcgccgggc gctcgagcac ctgtctcatg
    36541 gcgacgtcgc cgtccacccc agggatgcca cggccgtccg cctggccgcg ctcctccacg
    36601 acatcggtca ctacccgttt tcccatgctc tggaagaggc gggtctcccg aaacacgagg
    36661 ccctcgcgcg ccggcatctc accggtggtc agctggggga agtgctgggc aagatcggca
    36721 tcgatccgga aatcctgctc tcactcatcc agggccgggg gaccgtgccg ttggccggga
    36781 tggtcgcggg ctccctcgac gtcgacaagc tcgattacct gtcgcgcgac gcgtggatgt
    36841 gcggggtgcc gtacggcgtc atcgacgtgg accggctgct gtcctccctg acgatcgtcg
    36901 aaaagtcagg acgtcccacg ctggccctgc acgagaaggg tctggccgcg ctggagtccc
    36961 tgctcttcgc caagtatcag atgtaccgca acgtctactg gcaccacgcc gtgcgctccg
    37021 ccaccgtgat gttcaaacgt gccgtgacgg acgccatcgc ggcggggcga ctgtcgaccg
    37081 acgccgtcgc ggacgccacc gacgacgggc tgattcacga gctctcggcc aacgacccag
    37141 ccgggctggc ccgctcgctc cgcgaccggc gcctggcgaa gcgggccctc gacctgcccg
    37201 cgaccgacct tccgtccccc gccgactggc ccgcggccga cccggcgctg gtcgcgcagg
    37261 tggaggaccg cctcgcggcc gagctgaagc tcgcgcccgg cgatctgttc atcgatttcc
    37321 cggcgaagcc ggagatgctc gacctgcgcc tgccgctggt gacccgtgac ggcgaagtgg
    37381 tcgagctgga ggggcaagag gccgcggaac gcctgggatt gccccgcgtg tccgcggagc
    37441 tggtacgctc ggcacgccgc ctgcgcgtgt tcgtacggga gccccgcagc gtgccggaac
    37501 gggcggtggt gaacctcgtc acgttgcccg gcgccgaggt cgcgtcacga ctggccgggg
    37561 atcgttcact gctcagctga cgcaacaccg ctgcacggaa ctccagcgtc cctttcgcct
    37621 ataccagtca tgaacgcaaa gcttcgaatc gccgctggtg cgacccttct cggcctgctg
    37681 gccctggggg tcgcggatct gtctctgggc gccagcgtcc cccgttacct cgatctggaa
    37741 cggaccaacg cggagctcct gacgcaaatt cgggagacgc agggcacgtt gcagatcctc
    37801 agcgattcgg tggagtccgt cagccgccgg gcgcaggtcc tccgtctcct cgcgggcgtg
    37861 agcccgctgg attccggggt tgatctgccc ggtaccgatc cgtcatgggc ctgggcccag
    37921 cgcgacagtc ccgccgcgtg gtcccgcgac gtcacccgga cctccgccgc ccccacgacg
    37981 ccggaggagc tcgccctagc cggaacgctc ccggtcgcca tcacgcttac cgcgataccc
    38041 cctggccggg tgtcgagccc attttcggcg gaacgtctga gccccgtggt ggacacggtg
    38101 cggccgcatc aaggcgtgga cttcgcggct ccgccgggga cgcccatccg ggcgcccgcg
    38161 gccggcgtgg tcgtggaagt gcagtgggaa gagggctatg gggagtacct caccatcgat
    38221 cacgggcacg gggtcgtgac ccgatacgcg cattgctccg atatcctggt cgctccaggg
    38281 tctagagtcc ggcgcggcca gaagatcgcg ctggtcggct cgaccggaga agccacaggt
    38341 ccccacctgc acttcgaggt atgggtggac ggccgggcag tggaccccca gcgcttcgtc
    38401 gcgcccgggg acgtggtcac cgactgagtc ctacggagtc gtgtacgtga gtttccgggg
    38461 gggcacttca gccggggagc gtgttctctt ccgtggccct tccacctgcc ggcgtgtcaa
    38521 cctgatcgat ccgcaccatg atggcggtga tgttgtcgag cccaccgtgc cgattcgctt
    38581 cttcgatcag cgaatccacc tgctcctgcg gctgcagccc gttcttcatg atctccgcca
    38641 gttgcggatc ttcgagcatc cccgtcagtc cgtcagacgc cagcaggaac aagtcgcgct
    38701 cccggacgct cccgacatag acgtccggca tcacgtccgc attcgcgccg acgcagcggg
    38761 tgatcacgtt gctgtaggga tgcgtacggg cttgctcggg cgtgagatac ccggcatcga
    38821 cctgctcctg cacatacgag tgatccttcg tcacctgcgc gagcttgccg tcccgcagca
    38881 gataagcccg gctgtccccc acctggccga tcaggaagcg gttcgcgaag agcgtcagcg
    38941 ccgtgacggt ggtgcccatc ccgcgcttgt cgtgctcggt gagcgtgcgc tcgaagatgg
    39001 cgccattggc ggcgcggatg gagagccgca tccgctcggc gacctgttcg tcgttcaggc
    39061 cctggaggga gccgacttca cgggcaatga cgcgcaccgc catctcgctc gccacttccc
    39121 cggccgcgtg tccacccatg ccgtcggcca cgatgaaaac acccttgtcg gggaccatga
    39181 ggaagttgtc ctcattcccc gaccggatga tgccgacgtc ggtgcgaccc gcgcaggtga
    39241 tgtgcactcg gtgccttagc ggaggatcca gtacaacagg gaggcgccac caagaacgaa
    39301 catggccgcg ctggccccac atcccttttt cttcgccgga accggcggcg gcaccggttg
    39361 ccgcttcacc gcggggacgg gcgcaggagc cttcggggga ggcggcgtcg gagccgccgc
    39421 cttcggagcc ggagccgggg ccgcggaacc gggcttgaag gcctcgatca ctttggtccc
    39481 gccacccttg gccgtcccca tcgcgtcgtc ggtcggttcg aacacggcct gcacatcccc
    39541 gaaccgtacc atgacgcccg gcgacaacgg cgactccccc tggacctttt cgccgtcgac
    39601 gaaggtcccg ttcgtggagt cgaggtccac cagcatccat acgccctccc ggcgctgcag
    39661 cttcgcgtgc gacgtggaga ccgaggggtc agccaggacg aggtcgttat agtccgcgcg
    39721 accgatgttc aggaccggcg tcttgatctg atagcgctgg cccttcagcg gtccggtgcg
    39781 gaagaggaag ttgcccatcg cggcgggcgc gggcgaaccg gcgggggtgc tgggcgccgt
    39841 cgcggagggc cgtggtgccg ccgccatccc gaagctggtg ttgttcaacc ggtgtcccgc
    39901 gccgctcggg ggtggaggtg tctggggcgc ggcctgcggc ggactagcgg ccggccccgc
    39961 gggaggggag ggcggtgcct ggacggccgg ggcggcgtcg gcatagaagc ggaactgctc
    40021 gtccccgatc ctgatgacat ccgcacgcat cagcagacgc tgaccctcga tcttctcgtc
    40081 gttgacgaag gtgccattgg tgctcgagtc gaccaccatg tagcccttgg gggtggtcag
    40141 gatctccgcg tgacgccggg agacgtcctt ccccgtcacg accacatcgc agccggcctc
    40201 gcgtccgaag accagagagg cacctgtcac ggcgtactcg cgcccgtcgg tgagcgaaac
    40261 gatgcggccg ccggtgttgg cggtcggctt gcccgctccc gctgatgcct tggcagcggc
    40321 cgcggccttc gagacgtcca tcgcggccac gtattgtgtg cttccggaac gacgttcatc
    40381 cacgaacgtc agctcatggc caccggtttc gatcttgtcg ccgtgcagaa gaggcgtcgg
    40441 ttcggcaccg agattgacgc cattgatggc gatgatcgct tgcgggtcga ccttccggat
    40501 cgaaacttgg ccgtccggca accccttgag gattgccgtc ttgggggtga tcccggggcc
    40561 cgtcaattgg atcgcacaat cggcagcact gccgagcacc acttcgccgg caggaatcgt
    40621 gaactttctg ccgcccatct cgagaagggc catcgggggt tcctttctac gggaaggccg
    40681 gcacggaagt tacgcccggg tccgtaactg gacaaggcga cacgggttac gccccgtcgg
    40741 tgatctctgg gcgctggtcc gtcgacaccg cttcttccat tccgatgagc cccttccgaa
    40801 gccatcccca gaaaatgagg gcgccaacga tcacggtcgc gtaactgttg atgaatcgcc
    40861 agaccagggt atagctgggg atgagagggc gagggacgta aatggacatt acgaccgtcg
    40921 aaatcccctc ggccattcca gaaccacccg gcgtgggggc aaagtagagg acgaaggtga
    40981 tgagggtctg caccagcagg atctcgacga agtcagcggg gatgcccagg gcccgcatgg
    41041 cgacgtagcc ggcgagcagt ttattcgcat gtgacggggc cgacaggacg atggcccaga
    41101 aggtggcgag ccaccccttc gggctgccga aggccacgaa gcaccggtgg ccatattcga
    41161 tcccctcccg cagctgctcc atgcccgccg ccacccggcg gctccgcttc cccactctgc
    41221 ccgccagcca atgcaccagg tccttggcca gccgggggaa gaccatcaag aacagcatca
    41281 agaggccgag gaaaacgaaa acgcccaggg agacgcggaa gaggtcgtag agaccgatgc
    41341 ccaagacgat atggtgcttc gccaaggacc ggccggcacc gaaaaagatc gcgagcggcc
    41401 cggcaatcgc gaaaaacgcg accgtcgcaa cgaaggtcat gaaggtggcc gtgaaggcgg
    41461 cgggaagacg gactccggcg cgcttcatgg tccacatcat catcgggccg gcgcccgtct
    41521 ggaacggggt caggtagccg ccccaggcgc tcatgcctcc cgccacgatc atgtccctga
    41581 gtcggacgcc gggctggatg tgccgcgcca ccacccagag ccgcgtgccg ccgcctaccc
    41641 agtccatgga cgcgagcgcg aggccgattc cgacccaccg ccagtgaatg gaagcgaggg
    41701 cgtcgaggaa cgcccggagg ttgtcgccat agaaaagcag ggcgacgaca ccggccaggg
    41761 aaatgattgc gaatagggcg agtccccggc ggagcagccg aggggtcagg gcgagcgcca
    41821 tgtgaggccg aaggtaaggt tatgtttagg ggttatgcca gcccctcccg accaagtcgt
    41881 cctccgcaac gtgcgccagc acaatctgaa ggggatcacc gtctccctgc cccggcgggc
    41941 ctttacggtc attaccgggc cctcggggtc cgggaagagc tcgctggcgt tcgacaccct
    42001 ctacgccgag ggacagcggc gctacatcga atcgctgtcg tcctatgcca agcagtttct
    42061 ggagcggatg ccgaagcccc tgctggactc gctcgagggg ctttcaccgg ctgtcgccat
    42121 cgagcagaag aacccgacca cctcgagccg ctcgaccgtc gggaccgcca cggagatcta
    42181 cgacttcctc cgcctcatgt gggcgcggac cggaacgacc tactgccgca actgcgggaa
    42241 tgtggtgaaa gcagacaccg ttcaagacgt ggtcgacggt gtggtcggga gccgggagtc
    42301 gggagtcgtc gactccgtga cggtggcatt tccgctgccc ggagccggag atcgcgcaga
    42361 catcgagata gccgcccagc tccgggccgc gggattcgtg cgcgtccagc tcgacggcga
    42421 agtcacccgg ctcgacacgg acgatgccga atcccggatc cgggcggcga aggaagtgct
    42481 gatcctcgtg gaccgggtca gcgccagccc cgcgaaccgg ggccggctga ccgacgccgt
    42541 gtcgaccgcg ttcgcggagg gcgatggagt cgccctggtg cttaccgacg gcggccaccg
    42601 ccgccgattc tcggagcagc cggcctgctc caattgcggg cggccggctc cggttccgac
    42661 gcccaacctg ttctcgttca ataatccccg gggtgcctgt cccgggtgca acgggttcgg
    42721 cgcggtcctc aagtacgacg aatcattgat catccccgac cagcggctgt ccctggctga
    42781 aggcgccctc gacccgtgga ccaagccgcg gtacgaaggg cggcgccgga ttctgaggga
    42841 tgccgcgagg aaccggggcg tctccgtgga caccccctgg caagagattc cggcggggga
    42901 tcgggagttc ttcctgcacg gcaccaaggg ccgctttgtg gggatgtttc cgttcctcga
    42961 gcgcctcgaa gaaaagcggt acaagcagta catccgcgtc tttctgcgtc agtaccagct
    43021 tgccaagact tgcccccaat gcggcggcgc gcgcctccaa ccggacgcgt tggccgtgaa
    43081 agtggccggg aagacggtcg ctgaagtctc cgcccttacg gcgacggcac tgaccacctg
    43141 gctcaaggag ctctcgctcc ccgatttcca gcaagccatc gcggagcaca ttctcgggga
    43201 gttggtcgcc cgggtgcagt tcgtggtcga cgtgggattg ggatacctga cgctcgaccg
    43261 ccagactcga acgctgtcgg gtggcgaagc ccagcggatc gccctgtcca acgccctcgg
    43321 tgcccacctg gtggacacca cctacgtgct cgacgaaccc tccatcgggc tgcaccccgg
    43381 cgataccgac cgcctcctcg gcctcctccg ccgcctggcg gatgccggca acacggtcgt
    43441 cgtggtggaa catgacccgg ccgccatggc cgccgccgac tacatggtcg agctcggacc
    43501 ggggagcggc gagaatggcg ggcaggtggt ctaccagggc ccggcggctc gcgtgcgcga
    43561 agcgggaacg ctcaccggcc agtacttgtc cggcgaaaag cggatcggcg ttccctcggc
    43621 tcgccgtccc gccggtccgc gatggctgac ggtccgcggc gcgcggctgc acaacctcga
    43681 gggcgtcgac gtgcgcatcc ccctcggcac gttgacggtc gttacgggtg tctccggatc
    43741 cgggaagtcc acgctcgtcc atgacgtcat ctaccggcag ctcgaatccc gcatcaaggg
    43801 tgggcacagc gccaaggagc atctgggtga ggtcgtgggc gccgtctcgg gattggcggg
    43861 atgggagggg atcgccgacg tcgtgctggt cgaccagagt ccgatcggcc ggaccccccg
    43921 gtccaatccg gtcacgtacg tgaaagcctt cgatgagctg cgggcgctgt atgcgtccac
    43981 gccgttggcc cgggaacggg gctacacgcc gagtacgttc tccttcaacg tggcgggcgg
    44041 ccgttgcgaa gcgtgcgaag gagccgggca cgtgcaaatc gaaatggtgt ttctcgccga
    44101 cgtgttcgtc ccctgcgaga tttgcggcgg ccgccggtac aagcgcgaaa tcctcgacgt
    44161 caaagtggac gggcggtcga ttcacgaaac gctccagctc acggtcgacc aggctctgtc
    44221 catcttccac cgccaggggg gcctggcccg gacgctctgg cacctgcagc aagtgggact
    44281 cgggtatctg cgcctcgggc agccggcgac gacgttgtcg gggggcgagg cccagcgact
    44341 gaagatcgcg cgggagctct cgtccgcagg cggacggtcc cgaagaggcc ggaagttcta
    44401 cattttggac gagccgacca ccggcctgca tctcgacgac gtccgcacac tctgccgggt
    44461 gctcgaccgt ctggtggacg ccgggcatac cgtgctggtc attgagcatc acctggatgt
    44521 catcaagcgc gccgactggg taatcgacct gggacccgga gccggagcgg acggaggacg
    44581 tgtggtagct gaaggggacc cggaagcgat cgcccaagcg cgcggctccg tgaccgggcg
    44641 ctatctgcgg ggcggatcgt gaccagacgc caggccgatc tgctgctggt cttcacctgc
    44701 gccatctggg ggctttcgtt cccgacggtg aaggtcgcgc tcgcagacgc gtcgccactc
    44761 gcgttcgtgg ccatccgttt cacgctcggc gcgctgattc tcctcccggg cacccgcctt
    44821 cggcccctgc cttcacgcgg cgagtggttc gccggtctcc tgctgggtgc actcctcggc
    44881 gtggggttcg cgacccaggc ggtggggctc gcgtacacga ccgcctcacg ctcggcattc
    44941 atcgtggcgc tgtcgtcggt gctggcgccc ctggtcgcgt tcgtcgcggt ccgggagcgg
    45001 ctctcgtggt ggacagtggc cgcgctgggg ctggccaccg ccgggatcta cttcctcacc
    45061 gacccgggcg ccggcggatt gaatcgaggg gactggtgga cgctgatcac cgccctcgtc
    45121 ttcggggcgc aaatcgtggc cgtgcacgaa ctgggtcttc gcttcgacat ccggcgattg
    45181 gtattcatcg agatcgtcgt cgtggccgtc ggtgtcggcg tggccgcgcc cctgctggaa
    45241 ccggtgcggt tcgccgtcgc cacgcagtcc ctactcgcgt tggcgtactg cggggtggcg
    45301 gctaccgcgg tggcgttgct cctgcagatg cgggcgcaga aggacctgtc ctcggggcgg
    45361 gccgccctgc tcttctgtac ggaacccgtg ttcgcgagcc tggcgagctg gctctggctg
    45421 ggggagcggc tttcactgac gcagtgggtc ggggggggca tgattgtagt cgggatgttg
    45481 gtcgcggagc cgaaagaagc cgtcagccat cagccgtcag ccgtcagttg aactcgcgca
    45541 gccacccggt gatatttcac gccgtctgct gatagctgat ggctgtctgc tgacggcttt
    45601 attcccgagt agctcagtcg gtagagcagc cggctgttaa ccggcgggtc gggggttcga
    45661 gtccctcctc gggagctgtg cgacaccaga acggcctctc tgtcctgatt ttcaagggcg
    45721 ggaggccgtt cccgctggac ggctcccgaa cgggggccta gaatcgacga tatgccgcgc
    45781 aagccgaagg cgcgcccccc tcgcccccct catcctcccc acccgcccgc cccaccggaa
    45841 ggggacctcc gggaagcggt atccatcctg accgcgaccc tcgaatccac cgccgatggg
    45901 atcctcgtcg tgaaccggac gggggcgatc accacataca accagcgctt cctcgagatg
    45961 tggcgcattc cgccggcggt cgcgcagtcg cggaatgacg atcagctcat cgggttcgtg
    46021 ctcgatcaac tgcaagaccc gaacgtcttc ctcgcaaaag tgatggagct ctacggccgc
    46081 ccggaggcgg agagtcacga cgccatcctc ttcaaggatg gccgggtgtt cgaccgattc
    46141 tcggtgcctc aacgggtcgg cacgaaggtg atcggccggg tctggagctt ccgggacgtg
    46201 tcggaggtcc ggcgggccac cgaggagctg cgccggaacg aagaacggtt ccgggccctg
    46261 gtggagaaca gctccgacgt catcctgctg ctgcggcccg acggaaccat ccacttctcc
    46321 accgcggcgg cgttcccgat tctcggctac gaggccgggg agaacatcgg aaagtcgatc
    46381 ttcgacctga tccatcccga ggacctcccc cgggcgcgcg gggtgttcgg ggagctcatg
    46441 cgccgccccg gctccaaggt gtacctcgag tgccgggccc gccggaagga cggggactgg
    46501 ggcaatctcg aagcggtggg cgtgaatcgg ctcgacgatc catcggtcga ggcgatcatc
    46561 gtcaccacgc gcgatatctc ggatcgcaaa cgcgcggaac gggtccagca agcgacctac
    46621 cgcatcgcgg acgcggcgtc gatcgaacgc tccctcgaag acctcctccg cgccattcac
    46681 agtatcgtcg gcgagctgat gccggcggac aacttctacg tggcactgct cgagcctgac
    46741 ggtgaaagcg tcacgttccc ctatttcgtc gaccgcttcg acgcccagtt cgaacccttg
    46801 cccctcggga ggaacctgac gtcgtacgtc atccgctccg gtctcccgct cctggcgacg
    46861 tccgacgtat tcgaggaaat ggtcgcgcga ggcgatgtgg acgaagtggg agcgccctcc
    46921 atcgactggc tcggcgtccc gctcaccctc gagaaccgga ccatcggcgt cgtaacggtg
    46981 cagacgtact cggagggcgt ccggctcacg gaggcggaca aggacatcct gaccttcgtg
    47041 tcggcgcaga tcgcccgggc catcgaacgc cggcgggcgc aggacgcgct gcggcaggcc
    47101 gaggagcggt atcgcgcgtt cgtgacccag tcgaccgaag cgatctggcg ggtggagctc
    47161 gaccacccca ttccggtcga cctcccggag gaagagcagg tcaaccggct ctatcgggac
    47221 gcctacctgg ccgagtgcaa cgaggcgatg gtgcgcatgt atgggttcga gagcgtggac
    47281 caggccatcg gcgcgcgcct cggcaacttc atcatccagg agcatgcgtg gaatcgggag
    47341 acctggggca ccttcgtccg ttccggctac cggctgtcgg acatcgagtc cttcgagcgg
    47401 gaccgctcag gcgcccacaa gatattcctg aataaccgca tcggcatcgt ggctggcggc
    47461 catctgcgcc ggatctgggg cactcagcgg gatgtgaccg cccagcacca cctggaagac
    47521 cagctccgcc aggcccagaa aatggaagcg gtggggcggc tggccggggg catcgcccat
    47581 gacttcaaca atatcctgac cgccatcctg ggcacctgct ccctgatgca gcgggacctg
    47641 cccgccggat cgcggtcccg tgaggacgtc gacgaaatcc agcgctcggc cgcgagggcc
    47701 gcgggactga cccggcagct cctcgcgtat tcgcggcggc aggtactcca gccggaggtg
    47761 ctggacctga acgtcgtcgt cagcgagatg gacaaaatgc tgcagcggct gatcggcgaa
    47821 gacgtggagc tcgtgaccat tctgggggag gacctcgggc gggtcatggc cgacccaggc
    47881 cagatcgagc aggtgctgct gaacctcgcc gtgaacgctc gggacgccat gccccaggga
    47941 ggccggctca cgatccgcac ggccaaccgg agggacaccc ccgttcccgg ggcctccggc
    48001 caaccggtgt cgcgcgtgat gctggcgatc accgacaccg gcattggaat ggatgaccga
    48061 gtccgttcac acctgttcga accgttcttc accaccaaag aggtcggcaa gggaacgggg
    48121 ctggggttgg ccacggtata cggaatcgtg cgacagagtg gcgggtccat ctcggtcgag
    48181 tcggcgcccg gaaagggcag caccttctcc gtctttctcc cacgggtcga ggacgccagt
    48241 ttccggcggg cctccttgcc ggcggcgatt ccggcggact ccggtgagaa gcacgcgccc
    48301 tctgcacaca tcctcctggc ggaggacgag ccacgggtcc ggctgctgac ccagcgggta
    48361 ctggaggctc gtggctaccg ggtgctggct gcgaccgacg ggcaggtcgc cctggagctc
    48421 gcctcggacc ctgccctgaa gctcgacctg ctcgtcaccg acgtggtcat gcccggcatg
    48481 agcggccccg acctggcccg ccgtctgcgg gagtcgcgcc ccggactgcc cgtcgtgtat
    48541 atgtcggggt acgcggacca gggcctggcc cacgaagccg ccgccacggg tggcgccttt
    48601 ctctccaagc ccttcgaaac ggagacgctg gagcggctgg tgcaatccgc attggaacga
    48661 cgggcccaat agaccggagc gaccagggac gtgcaaatcc tcatcgtcga agacgagtct
    48721 gtcacccggg aagtcctcaa gcggctgctc ctggccgcgg gccacgaagt taccgacgcg
    48781 gcggacgggg ccgaagcctg ggaactcctg cagtctatcc ggatcccggt ggtcatttcc
    48841 gactgggaga tgccgcagat ggagggcctg gatctgtgcc ggaagatccg aaaccggtcg
    48901 ggagagtaca cctatttcat catgctgacg gcgaaaagcg ggcgggcgga gtacctcgaa
    48961 gcgatggatg ggggcgtaga cgacttcctc gccaagccgc tcgacgccgt cgacctgcag
    49021 tgccggcttc gggtcgctga gcgtatcctc ggccttcgga gggaagtgca gcaactggag
    49081 ggtctgcttc cgatttgcag ttactgcaaa cggattcggg atgagatgaa ccgctggaca
    49141 tcgatcgaga cctacatcga acagcgttcc cgggcggaat tcagccacgg catttgcccg
    49201 gattgctaca agaaacacgt cgagccggta ctgaatccag gtcaataaat gccgtagata
    49261 caccgaaaaa actagcaatt atctgacgaa atcggcgatt gcgtgctcac tttttcatat
    49321 aactcattat tgaacatatt attatggtag cgcaatacct gtccagtagt gatataccgt
    49381 tgtaacgtaa cgagttgaca aatgaactcg gggcgccaag gccccctgcc gctagatttc
    49441 cgacaatgcc actcccccct atcgctggcc acgaagcgct gcgggcgcgc ttcgccgatg
    49501 caatccgggc cggccgtctc ccacagactc tgttgttcga gggccccagg ggggtcggga
    49561 agcagcgctt ggcgttgtgg ctggcccaga cgcttctctg cgaaacggcg tctgcgccat
    49621 gcggaaaatg cgcgtcgtgc cggcaagtcg aaaacctgag ccacccggat ctgcactggt
    49681 tcgtcccggt tgagctcacg aaaaaaggcg ccgacgcgga caagcagatc gacctcgtcg
    49741 aagaggcgat cggcgaggtg ttggccgagc gtcgtgaaga gccgttctac gcgcccccgg
    49801 cgggactggc ggcgcacagc atggcgtcgg cgcgcctgat gctcaggcgt ctcgcactga
    49861 agccggccca gggttcacgg gctgtcttca tcctgggcga tgcggagcgc ctggcggcgc
    49921 agaagggtca ggacggagcg gcaaacgcgg tgctcaaggc actcgaggag ccgccgccga
    49981 cagccttcat catcctgacg acgagtgaac ccgacgcgct cttgccgacg attctctccc
    50041 ggacggttcg ggtaagggta ggcaggttgc cagacagtgt tgtgacatca ttcgtacagc
    50101 aacacatggt gtcacgccta ggggacgccg caagcgtcgc gcggcgggta cagggtgccg
    50161 agggagcgat tggcaggcta gttgcgaccg agggaagcga ggggagcggg gccgccgcag
    50221 cggaccgact cctgcaagcc atacggggtg gaacggccgc gcggtatgga gcggcgctga
    50281 cgcagccccc gttccaggcg cgcggagcat tcacctcgat gctcgatgcg ctcctcgaac
    50341 gcctccggga cgaagcccgt gagggtggcg acacgagcaa agtgatggcg gcgatgacac
    50401 gggtgctcga agcgcgggag ctggcccagg gcaatgtgaa tcctcagctc ctgacggccg
    50461 tcttgactga ggacctggcc ggtggagggc gatcgtgaag ctctcgcacc tggatcagga
    50521 aggccgcgcc cgcatggtgg acgtcagcgg gaagcccgcg acggcccgca gtgcggtggc
    50581 ggaaggccgc atccggatgt cggaggaggc gttcgggctg gtccgggacc aggcggtggc
    50641 gaagggcgac gtcctcggcg tcgcgcaggt ggcggggacg atggcggcca agcggacggc
    50701 cgagttgatc ccgctctgcc acccgctggc actcgaccac atcgccgtcg aggcgacgct
    50761 ggacgaggcg ctaccgggag tacaggtccg ggcgtcggcc cggatggtgg gccggaccgg
    50821 ggtggaaatg gaagtcctga cggccgtgag cgtcgcgctc ctgaccgtct atgacatggt
    50881 gaaggcggtg gaccggcata tggcaatcgg gccggtgcac ttggttgaga agcatggcgg
    50941 gacctccgcc gaagcttgac ggagttttga accggggatg gcaggttgtc cccgagacag
    51001 ttcttcacaa gagaggtata tgcgcaccac gacccagcga ctgatgctgc gggggacggt
    51061 gatcgtaggg ttcgcgttgg cggtgagcgc cgtcggcgga tgggtccgtc gcgccactgc
    51121 ggacgacgtg accctccccg aagccacggt tccggccgcg gacgtgcgag gcatggaaca
    51181 gactctggat caggtccagg gtgagctgac ggtggccaag ctgcagttgg agcgggcgac
    51241 cgccatcatc gggttttcca gcaagtacgg aattcccgcg gacctggcca gcaatattta
    51301 cga
//
LOCUS       DASUBP010000187         5536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_795830, whole genome shotgun sequence.
ACCESSION   DASUBP010000187 DASUBP010000000
VERSION     DASUBP010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5536
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_795830"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..338)
                     /locus_tag="VFU75_07130"
     CDS             complement(<1..338)
                     /locus_tag="VFU75_07130"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEU5466646.1"
                     /translation="MSGKRPIDAVDRQLLRLLADNGRASFQALADEVGLSRPAVMERV
                     RRLESAGIITGYSAQLDRAKAGVPVTAFVAVRYGSSGYVGDEPRMRSLLKHPGVLEAH
                     HVAGDDCYVL"
     gene            496..1296
                     /gene="larE"
                     /locus_tag="VFU75_07135"
     CDS             496..1296
                     /gene="larE"
                     /locus_tag="VFU75_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HEU5466647.1"
                     /translation="MSLSRLREVVRAYPSALLGYSGGVDSALLAVVLRQELGRDRMLA
                     AIGRSDSYPEVQWRVAHEIAGRFDVPVLEVLTRELDDPRYLANPTNRCFYCKTELWGR
                     LSRVAAERGLAVICDGSNADDLGEHRPGRAAGTAAGVRSPLAEAGLTKAEIRAASADL
                     GLPNWDAPAAPCLSSRIQYGLAITPTRLKQVEAGEAYLRAEGVTGDLRVRHTGTAARI
                     EVDPAAFALVEARIETIRRRFQELGFTTVEVDPRGYRRGSLLLDRAAP"
     gene            1311..1859
                     /gene="thpR"
                     /locus_tag="VFU75_07140"
     CDS             1311..1859
                     /gene="thpR"
                     /locus_tag="VFU75_07140"
                     /inference="COORDINATES: protein motif:HMM:NF024952.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HEU5466648.1"
                     /translation="MNFPATLRAGLWEATEPLRAMHLPVRWVAPDGMHLTLKFLGETD
                     EQRLGELGDALDRAVAGVRSMPVTVQGFGAFPNAAHPSVIWAGVVTDPALELLQHGVE
                     QAFGPLGFPPEGRPFRPHVTLGRLGRSRRDSPQGAFRVLEETLDALVWSETAEVTSVE
                     LMKSTTMKGGAVYEEVRHGRLS"
     gene            1843..2502
                     /locus_tag="VFU75_07145"
     CDS             1843..2502
                     /locus_tag="VFU75_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466649.1"
                     /translation="MDGCLKAIGRLGCLLAIVIAAGVAYWFRAPIMHTASRYLGPHST
                     RLPPVSDTSVGAPTPGAVRAVAAKLASLGAPGGPDSVVLTPNEMASLIGGGIDWTVRK
                     GFDSLRVELMDGSIAVDARLDTRVLPRDALGPAEHVLATYEPLRMAGPVTIGTPGVAH
                     WKIQELSVHGFPFPSPAVNRLAKLIAGADDGSVPVLVPTTIRRIAINPTGVVCYRRSK
                     A"
     gene            2499..3887
                     /locus_tag="VFU75_07150"
     CDS             2499..3887
                     /locus_tag="VFU75_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5466650.1"
                     /translation="MTQRVLVADDEKGIREALTQLLEFENLEVMSCSSGGDLLLKYPE
                     FRPHLVFLDVKMQGMDGLETLRKLRELDPHAQVVMISGHGTVQTAVEATQLGAYDFLE
                     KPLDTDRVLLTLRNALQHGALADENVRLKAEVQSRHQIVGSSKAIKQVLDTIEKVAPT
                     PARVLITGENGTGKELVARAIHNLSPRADGPFVEVNCAAIPTELIESELFGHVKGSFT
                     GAVGDRAGKFELADGGTLFLDEIGDMSLSAQAKVLRALQEGVISRVGSGKTLAVDVRV
                     IAATNKKLDSEIAAGRFREDLLYRLNVVPIVVPPLRERRGDIPQLVRHFVEELGRATG
                     LPSRGFEEGALEHLAQHDWPGNIRELKNAVERLLILAPGRAVTAADVERIATGPSGGT
                     GAGVGIAGGNGGALFQSATFEEFKLAAEKAFLVAKLQEHDWNVSETARTLEMPRSNLY
                     KKIERYGLVREG"
     gene            3888..4355
                     /locus_tag="VFU75_07155"
     CDS             3888..4355
                     /locus_tag="VFU75_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466651.1"
                     /translation="MPGKDERDRDWDKELAEVDRLLNNMPGHPPAPAPIKPAGGYPPP
                     GQAHAGHGPRVLSTREWAGTWIRVGLGLLIGIGMTQWPYTHGCGFKLGFYLLGVAAVL
                     TAGVWSSISSWRRHLPWAHILSQGLVIWGLTLAAREILPRVHYAREIATWMCP"
     gene            4394..>5536
                     /locus_tag="VFU75_07160"
     CDS             4394..>5536
                     /locus_tag="VFU75_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5466652.1"
                     /translation="MTDFKNFIAGAWVAPVTGDYFDNTNPADTSDLIGRFPQSGAEDV
                     GRAAASAKKGFAAWSRTPAPIRGQVLQRVGDLLVKKKEEFARAMTREMGKVLLETRGD
                     VQEGIDTAYYAHTEGRRLYGKTVPSELRNKWAMSFRRPIGTVGVITPFNFPLAVPTWK
                     IFPALLCGNAVIWKPSEDVPHTAHLLVELLLEAGLPPEVIQLVHGGGAAGKALVEHPD
                     VGVISFTGSTETGALIGATCGRMHKRLSLEMGGKNAMIVMDDADLELALDGVLWGAFG
                     TTGQRCTATSRLILQSGIHDRFVKMVADRAQKLRLGPGLDAQTEVGPLIHEASRNKVE
                     EYVGIAKEQGAAVLIGGAKATGKGLERGWFYQPTLFVGVKPGMRVEQ"
BASE COUNT          882 a         1842 c         1902 g          910 t
ORIGIN      
        1 ttgaggacat agcagtcgtc gccggccacg tggtgagctt cgagtactcc gggatgcttg
       61 aggagtgacc gcatgcgcgg ctcgtcgccg acgtagcccg agctgccgta gcggaccgcg
      121 acgaacgcgg tgacgggaac cccggccttg gcgcggtcga gctgggccga atacccggtg
      181 atgatgcccg cactttcgag tcgccggacc cgctccatca ccgcgggccg ggagagtccg
      241 acttcatccg cgagggcctg gaagctggcc cggccgttgt cggcgagcag gcggagcagc
      301 tggcgatcca ccgcatcgat cggtcgtttg cccgacattt cgctagctcc tcggaatcat
      361 gactgacgat tagtaggtca cggagatagt tcaataacgt ttcatcatag taattggttt
      421 ccgcgggcgg gactatccgg ttccggtccc ggaacccggg gtacgtcggc acctccgcct
      481 cactactatt cggccatgag tctcagtcgc ctgcgcgagg tggtgcgcgc ctatccttcc
      541 gctctcctcg ggtactccgg tggtgtcgat tccgccctgc tcgccgtggt gctccgccag
      601 gagctcggac gggaccggat gctcgccgca attggacgca gcgattcgta tcccgaagta
      661 cagtggcgcg tggcgcacga gatcgccggc cggttcgacg tgccggtgct cgaagtcctc
      721 actcgtgagc tcgacgaccc ccgctacctc gccaacccga ccaaccgctg cttctactgc
      781 aagaccgagc tgtgggggcg cctgtcgcgg gtggcggcgg agcggggact cgcggtgatc
      841 tgcgacgggt cgaacgccga cgacctcggg gagcatcgcc ccggccgggc cgcgggtacc
      901 gccgccggcg tccgctcacc gctcgccgag gcgggtctga cgaaggcgga gattcgcgcc
      961 gcgtctgccg atcttggcct tcccaactgg gacgccccgg cggctccctg cctgtcgtcg
     1021 cggatccagt acggcctggc gatcacgccc acccgcttga aacaagtcga agctggcgag
     1081 gcgtatctcc gggccgaggg cgtcacgggg gatctgcggg tgcggcacac cggcacggcg
     1141 gcgcgcatcg aagtcgaccc ggcggccttc gccttggtgg aggcccggat cgagacgatt
     1201 cgccggcgct tccaggagct ggggttcacc accgttgagg tcgatccccg gggctatcgc
     1261 cggggcagtc tcctgctcga tcgcgccgcc ccgtgagatt gttcgtggcc gtgaacttcc
     1321 cggccacgtt gcgcgcaggt ttgtgggaag ccacggagcc gctgcgagcg atgcacctcc
     1381 ccgtccgctg ggtcgccccg gacggcatgc acctgaccct caagtttctc ggcgagacgg
     1441 acgagcagcg cctcggagaa ctgggagacg cgctcgacag ggcggtggca ggcgtgcgct
     1501 cgatgccggt caccgtacag ggattcggag cctttcccaa cgcggcccat ccgtccgtga
     1561 tctgggccgg cgtcgtcacc gatcccgccc tcgagctgtt gcagcacggg gtggagcagg
     1621 cgttcggccc cttggggttc cctccggaag ggcgcccgtt caggccccat gtcaccctgg
     1681 gccgcctggg ccgttcccgg cgagacagcc cccagggggc ctttcgggta ctggaagaga
     1741 ccctggacgc cctcgtctgg agtgaaaccg cggaagtgac ctccgtcgaa ttgatgaaga
     1801 gcaccaccat gaagggcggg gcggtgtacg aggaggtacg gcatggacgg ctgtcttaaa
     1861 gccatcgggc gcctcggctg cctgctcgcg atcgtcatcg cggcgggggt cgcgtactgg
     1921 ttccgggcgc cgatcatgca caccgcctcc agatacctgg ggccccattc gacccggttg
     1981 ccacccgttt ccgacacgtc cgtcggcgct cccaccccgg gagccgtgcg cgccgtggcc
     2041 gcgaaactgg catccctcgg tgcccccggg ggccccgatt cggtggtcct gacgccgaac
     2101 gagatggcgt cgctgatcgg cgggggaatc gactggaccg tgcggaaggg gttcgactcc
     2161 cttcgggtcg agctgatgga cggcagtatc gccgtggacg cgcgtctcga tacgcgcgtc
     2221 ttgccccgcg atgcgttggg gccagcggag cacgtgctcg cgacgtatga gcctttacgg
     2281 atggcggggc cggtgaccat cggcacaccc ggcgtcgcgc actggaagat ccaggagctg
     2341 tcggtccacg gattcccgtt cccgtcgccg gccgtgaacc ggctggcgaa gctgatcgcc
     2401 ggcgccgacg acgggagcgt gccggtgctc gtcccgacga ccatccgccg gatcgcgatc
     2461 aaccccacgg gcgtggtgtg ctaccggagg tccaaggcgt gactcaacgc gtgctggtgg
     2521 ccgatgacga gaaagggatt cgcgaggcgc tgacccagct cctcgagttc gagaatctcg
     2581 aggttatgag ctgctcgtcc ggtggcgacc tcctcctcaa gtatccggag ttccgccccc
     2641 acctcgtttt cctcgacgtg aagatgcagg ggatggatgg gctcgagacc ctccgcaagt
     2701 tgcgggagct cgatcctcac gcgcaggtgg tcatgatctc cgggcacggc accgtgcaga
     2761 cggccgtcga agccacgcag ctcggcgcgt acgacttcct ggaaaagccg ctcgacaccg
     2821 accgcgtgct cctcaccttg cgcaacgcgc tgcagcacgg cgccctggcc gacgagaacg
     2881 tccgtctgaa ggcggaagtg cagtcgcggc accagatcgt cgggtcgtcc aaggccatca
     2941 agcaggtgct ggacaccatc gagaaagtcg cgcccacgcc cgcccgggtc ctgatcacgg
     3001 gcgagaatgg gacgggcaag gagctcgtcg cccgggcgat tcacaacctg tctccgcgcg
     3061 ccgacggccc cttcgtggaa gtgaattgcg cggccattcc caccgagctg atcgaaagcg
     3121 agctcttcgg gcacgtgaag gggtcgttca cgggcgcggt gggggaccgc gcgggcaagt
     3181 tcgagctggc cgatggcggc acgctgttcc tcgacgaaat cggcgacatg agcctctcgg
     3241 cgcaggccaa ggtgctgcga gcgctgcagg agggcgtgat ctcccgggtc ggatcgggca
     3301 agacgctggc ggtggacgtc cgggtcatcg ccgcgaccaa caagaagctc gactcggaaa
     3361 tcgcagcggg acgtttccgc gaagatctgc tctatcgcct caacgtcgtg cccatcgtcg
     3421 tcccccccct gcgtgagcgg cggggcgaca tcccgcagct cgtccggcat ttcgtcgaag
     3481 agctggggcg ggccaccggt ctcccgtcgc gcggcttcga ggaaggggcg ctcgagcatc
     3541 tggcgcagca tgactggccc ggcaacattc gcgagctgaa gaacgcggtc gaacggctgc
     3601 tcattctcgc gccggggcgg gcggtgacgg ccgccgacgt ggaacggatc gccaccggcc
     3661 ccagtggggg aacgggggca ggcgtgggca tcgcgggagg gaatgggggc gccctgttcc
     3721 agtccgccac tttcgaggag ttcaagctgg ccgcggagaa ggcgttcctg gtcgccaagc
     3781 tgcaggagca cgactggaat gtctccgaaa ccgcgcgcac attggagatg ccgcgcagca
     3841 acctctacaa gaaaatcgag cgctacggtc tggtacggga gggctgagtg cctgggaagg
     3901 atgagcgcga ccgcgactgg gacaaggagc tggccgaggt cgatcggctg ctcaacaaca
     3961 tgccggggca ccctccggct cctgccccca tcaagccggc cggtggttac cctcctcccg
     4021 gtcaagcgca cgccgggcac ggtccccgcg tgctctccac gcgggaatgg gccggcacct
     4081 ggatccgggt gggactcggc ctcctcatcg gcatcgggat gacgcaatgg ccgtacacgc
     4141 acggctgcgg attcaagctc gggttctatc tcctgggcgt cgccgcggtg ctcaccgcgg
     4201 gagtctggag ctcgatctcc tcctggcgcc gtcacctccc gtgggcccac atcctctccc
     4261 agggactcgt gatctggggc ctgaccttgg ccgctcgtga gatcctgccc cgagtacatt
     4321 acgcccgcga gatcgcgacg tggatgtgtc cctgacccct gacccctgac tcctgacccc
     4381 tgacccctct ttcatgaccg actttaagaa cttcatcgcc ggcgcctggg ttgcccccgt
     4441 caccggcgac tatttcgaca acaccaatcc cgccgatacc tccgacctga tcggtcggtt
     4501 tccccagtcc ggtgccgagg acgtcggccg cgcggcggca tcggccaaga agggattcgc
     4561 cgcgtggtcc agaaccccgg cgcccatccg ggggcaggtg ctccagcggg tcggtgatct
     4621 cctggtcaag aagaaggaag agttcgcccg ggcgatgacc cgggaaatgg ggaaagtgct
     4681 gctcgagacc cgcggcgacg tgcaggaagg catcgacacc gcctactacg cgcataccga
     4741 gggccgccgg ctgtacggga agaccgtccc ctcggagctc cggaacaagt gggcgatgag
     4801 tttccgccgc cccatcggga ccgttggggt catcacccca ttcaactttc cgctcgccgt
     4861 gccgacgtgg aagatcttcc cggccctgct gtgcggaaat gcggtgatct ggaagccctc
     4921 cgaggacgtc ccgcacaccg ctcatctgct ggtggagctg ttgctcgagg cgggcctccc
     4981 gcccgaggtg atccagctgg tgcatggcgg cggtgcggcg gggaaagcgc tcgtcgagca
     5041 ccccgacgtc ggcgtgatct cgttcaccgg atcgacggag accggcgcgc tgatcggtgc
     5101 gacctgcggc cggatgcaca agcgcctgtc gctcgagatg ggtggcaaga acgcgatgat
     5161 cgtcatggac gacgccgacc tcgagctcgc cctcgacggg gtgctctggg gcgcgttcgg
     5221 cacgaccggg cagcgttgca cggccaccag ccggctcatt ctccagtccg gcatccacga
     5281 ccgcttcgtc aagatggtgg cggaccgagc gcaaaaactg cggcttggtc ccgggctcga
     5341 cgctcagacc gaagtgggac cgctcatcca cgaggcttcc cgcaacaagg tcgaggagta
     5401 cgtcggcatc gcgaaggagc agggcgcggc ggtcctgatc ggcggtgcca aggcgacggg
     5461 gaaggggctc gaacgcgggt ggttctatca gcccacgctg ttcgtagggg tgaaacccgg
     5521 catgcgggtg gagcag
//
LOCUS       DASUBP010000188         4472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_986409, whole genome shotgun sequence.
ACCESSION   DASUBP010000188 DASUBP010000000
VERSION     DASUBP010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4472
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986409"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2006)
                     /gene="ppdK"
                     /locus_tag="VFU75_07165"
     CDS             complement(<1..2006)
                     /gene="ppdK"
                     /locus_tag="VFU75_07165"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HEU5466653.1"
                     /translation="MPSVERFVYSFGNGRADGSAAMRDILGGKGAGLAEMANLGLPVP
                     PGFTISAKICIEYLEAGAFSPELREEVERHLKQLEQMAGKRLGDVENPLLVSVRSGAA
                     VSMPGMMETILNLGLNDQTVQGLISGSKNPRFAYDSYRRFVQMYGDVVYELGKEPFEE
                     VLDEYKKKRKVERDIDLPAEDLAALVREFKDIIQAKRGTPFPDDPQEQLWGAIEAVFK
                     SWHTRRAIDYRKVHNIPDDLGTAVNVVSMVYGNMGDDCGTGVAFTRNCSTGENVLNGD
                     YLTNAQGEDVVSGARTPESITRMKRGKLAKVYKELERIAEKLEHHFKDVQDIEFTFEH
                     GKLYMLQTRRAQRTGMAAVRVAVEMVEEKRISEDEAVQRVPPQDLDQLFHPMVDPKAA
                     VTVIAKGLPASPGAAIGEVVFDADEAEALAKKGHKVILVRPETSPEDFHGIVAAQAVL
                     TARGGMTSHAAVVARGMGKTCVVGAKELDVQPAAGRFSANGRTVKKGQIVTVDGTSGR
                     VILGAAKVVAPKAGKHYDILMKWADARRRLRVRTNADTPADAKRAREFGAEGIGLCRT
                     EHMFFEGHRITAMREMIVATDETGRRKALAKLLPMQRSDFEGIFEAMAGLPVTIRLLD
                     PPLHEFLPHTKAEIAELAKAMKMPAAQLARIVANLVEANPMLGHR"
     gene            complement(2061..3455)
                     /gene="purF"
                     /locus_tag="VFU75_07170"
     CDS             complement(2061..3455)
                     /gene="purF"
                     /locus_tag="VFU75_07170"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEU5466654.1"
                     /translation="MCGIIGVSGVPDAARVAYLGLYSLQHRGQESSGIVAVDGEGVAR
                     SHRGMGLVSENFNDQVLASLTGDVAIGHTRYSTAGSSILANAQPFLARYREGPLALGH
                     NGNLTNAVPLRADLVAKGSIFQTTSDSEVLVHLIARSEAREPEDQILDALERVDGAYS
                     LVLTIGRTLYALVDPRGFRPLILGKLGPGYVVASETCALDLIGAEVVRELEPGEMLRI
                     HEGEVVRLPNLPKKASQRCIFELVYFSRPDSTIFHRSVDGVRRALGRELAKEHPAPGA
                     DCVFSVPDSSNAMALGFSEQSGIKLEHALIRNHYVGRTFIQPSQAGRNAKVKIKFNPV
                     REILEGKNVVIVDDSLVRGTTSRGLVQMIRQAGAKEVHFRIASPPITGPCWYGIDTPS
                     KSELIAANNTVEQIRKHLGVESLGYLSLDGMLRAAGGDPKEFCHACFSGAYPTPIPDE
                     SARGRYELPVAVPV"
     gene            complement(3462..>4472)
                     /locus_tag="VFU75_07175"
     CDS             complement(3462..>4472)
                     /locus_tag="VFU75_07175"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HEU5466655.1"
                     /translation="PSIASKRWVYEQYDSSVQARAVIHPGGDAGVLRVQGTDFGIAVT
                     TDCNSRYLALDPYEGGKGAVAEAALNIACTGARPLGITDCLNFGSPERPEVFFQFREA
                     CRGITDACRAFDTPVTGGNVSFYNESPTGAVDPTPVIGMVGLLDHVDRAVHSHARGPA
                     DHVLIAGTTSEELGGSAYWQTVYEFRGGTPPHVDLAQSRRLVDFLVRAAADSLLSSAH
                     DVSGGGLAVALAEIAMGGPYQPAGFGLNVNLSRYAPGLDTGALLFSESHARAVLTVPP
                     SQIHLVTALASEIGVPLFNAGTTGGAEGLFQVTLRDGKISYPVGKLREVYFGSIPRRM
                     GD"
BASE COUNT          727 a         1473 c         1506 g          766 t
ORIGIN      
        1 cggtgaccga gcatgggatt cgcctcgacg agattggcga cgatccgcgc cagctgcgct
       61 gccggcatct tcatcgcctt ggccagctcc gcgatttccg ccttggtatg gggcagaaac
      121 tcgtggagcg ggggatcgag cagccggatc gtgaccggca gtcccgccat tgcctcgaag
      181 atcccctcga aatccgaacg ctgcatcggc aggagcttgg cgagcgcttt gcgacggccg
      241 gtctcgtcgg tggcgacgat catctcccgc atggcggtga tccggtgccc ctcgaagaac
      301 atgtgctccg tccggcacaa cccgatgccc tcggcgccga actcccgggc gcgcttcgcg
      361 tcggccggcg tatcggcatt ggtccggacc ctcaggcgac gccgggcatc ggcccacttc
      421 atcaggatgt catagtgctt cccggccttg ggagcgacca ccttcgccgc gccaagaatg
      481 acccggcccg acgtcccgtc gacggtcacg atctgcccct tcttgaccgt cctgccgttg
      541 gcgctgaagc gccccgcggc aggctggacg tcgagctcct tggctccgac gacgcacgtc
      601 ttacccatgc cgcgggcgac gacggctgcg tgactggtca tgccgccgcg agcagtgagc
      661 accgcctgcg cggccacgat gccgtggaag tcctcgggcg aggtctccgg tctgaccagg
      721 atgaccttgt gccctttctt cgcgagcgcc tcggcctcgt cggcgtcgaa gacgacttcg
      781 ccgatggccg cccccggtga ggcagggagt cccttggcga tgaccgtgac ggccgccttc
      841 ggatccacca tgggatggaa gagctggtcc aggtcctggg ggggcacccg ctggaccgcc
      901 tcgtcctcgc tgatccgctt ctcttccacc atctcgactg cgactcgcac ggccgccatg
      961 ccggtccgct gggcacggcg agtctggagc atgtacagct tgccatgctc gaaggtgaat
     1021 tcgatgtcct gcacatcctt gaagtggtgc tcgagctttt cggcgatccg ctccagctcc
     1081 ttgtagacct tcgcgagctt gccccgcttc atcctggtga tggactccgg cgtccgggcc
     1141 ccggagacga cgtcttcccc ctgcgcgttg gtgaggtagt cgccgttcag gacgttctcg
     1201 ccggtgctgc aattcctcgt gaatgccacg ccggtgccgc agtcgtctcc catgttgcca
     1261 tagaccatcg acaccacgtt gacggcggtg ccgaggtcgt cggggatgtt gtggaccttg
     1321 cggtagtcga tggcgcggcg agtgtgccag ctcttgaaga ccgcctcgat ggcgccccag
     1381 agctgttcct ggggatcgtc ggggaaggga gtgccgcgct tggcctggat gatgtccttg
     1441 aactcgcgca ccagcgccgc caggtcctcg gcgggcagat cgatgtcgcg ctcgactttc
     1501 cgcttcttct tgtactcgtc caggacctcc tcgaacggct ccttgcccag ctcgtagacc
     1561 acgtcgccgt acatctggac gaaccggcgg taggagtcgt acgcgaagcg cgggttcttc
     1621 gaccccgaga tcagcccctg cacggtctgg tcgttcagtc cgaggttgag gatggtctcc
     1681 atcatcccgg gcatggacac ggcggcgccg gagcggaccg acaccagcag gggattctcg
     1741 acgtccccca gtcgtttgcc cgccatctgc tccagctgct tcagatgccg ctcgacctct
     1801 tcccggagct cgggggagaa cgctccggcc tcgaggtact cgatgcagat cttggcggag
     1861 atggtgaaac cgggtgggac gggaaggccc aggttggcca tttcggccag cccggcgccc
     1921 ttgccgccca ggatgtcgcg catggcggcg gagccgtcgg cccggccgtt cccgaaggaa
     1981 tagacgaagc gttccaccga gggcatcggt gtctccaaag actagacggt tagacggcta
     2041 gacggctaga caaccacgag ctagacggga acggcgaccg gcagctcgta gcgcccacgg
     2101 gccgactcat ccggaatcgg cgtgggatag gctcctgaga aacacgcgtg acagaactct
     2161 ttggggtcgc cgccggcggc gcgcaacatc ccatcgagtg agagatagcc gagagactca
     2221 acgccgaggt gcttgcgaat ctgctcgacg gtgttgttcg cggcgatcaa ctcggatttc
     2281 gagggtgtat cgatgccata ccagcagggg ccggtgatcg ggggggaggc gatccggaaa
     2341 tgcacttcct tggcgccggc ctgccggatc atctgcacca ggccgcgcga cgtcgtgccg
     2401 cggacgagcg agtcgtccac gatcacgacg ttcttgcctt cgaggatttc gcgaaccggg
     2461 ttgaacttga tcttgacctt ggcgttccgg cccgcttgcg acggctgaat gaacgtccgg
     2521 cccacgtagt ggttgcggat cagcgcgtgt tccagcttga tgcccgactg ctcggagaag
     2581 ccgagcgcca tggcgttgga agagtcggga accgagaaga cgcagtcggc gcccggagcg
     2641 ggatgttcct tcgccagctc acggccgagg gcgcggcgga ccccgtcgac ggaccggtgg
     2701 aagatcgtgg agtcggggcg cgagaaatac accagctcga agatgcaccg ctgggacgcc
     2761 ttcttcggca ggttcggaag ccgcacgact tcgccttcgt gaatcctcag catctccccc
     2821 ggttcgagct cccggacaac ctcggccccg atcagatcga gcgcacaggt ctccgacgcc
     2881 acgacgtacc cgggacccag cttgccgagg atcagggggc ggaagccgcg ggggtcgacg
     2941 agcgcgtaga gcgtccggcc gatggtgagc accagcgagt acgcgccatc gacgcgttcc
     3001 agcgcatcga ggatctggtc ttccggctcg cgggcttcgg aacgcgcgat caggtggacc
     3061 aggacttccg agtccgacgt ggtctggaag atggagccct tggccacgag gtcggcccgg
     3121 agcggcacgg cgttggtgag gttgccgttg tgccccaggg ccagcgggcc ctcgcgatag
     3181 cgggcgagga agggctgggc gttggcgaga atcgaggagc cggcggtgga gtaccgcgtg
     3241 tggccgatgg cgacatcccc cgtgagagac gccagcacct ggtcattgaa gttctcggaa
     3301 acgagtccca ttccgcggtg ggatcgcgca acaccttcac catcgaccgc gacgatcccc
     3361 gacgactcct ggccgcggtg ctggaggctg tagagcccca ggtacgcgac gcgggctgcg
     3421 tcaggaacgc cggaaacgcc gatgatgccg cacatctcgt ctcagtctcc catgcgccgg
     3481 gggatggacc cgaaatatac ctcgcggagc ttgccgaccg gatatgagat cttgccgtcc
     3541 cggagggtga cctggaacaa cccctccgca ccgcccgtgg ttcccgcgtt gaacaggggc
     3601 acgccgattt cggaggccag cgccgtcacc aggtggatct ggctgggcgg cacggtgagg
     3661 accgcccggg cgtgggattc gctgaagagc agggcaccgg tgtccaggcc cggtgcgtac
     3721 cgggagaggt tcacgttgag gccgaacccc gcgggctgat acggtccgcc catggcgatc
     3781 tccgcgagcg ccacggcgag ccctcccccc gagacgtcgt gcgccgagct gagcagactg
     3841 tccgccgccg cgcggaccag gaaatccacc agccggcgcg actgggcgag gtcgacgtgc
     3901 ggcggcgtgc cccctcggaa ctcgtacacg gtctgccagt aggccgaccc acccagctcc
     3961 tcgctggtgg tgcccgcgat cagcacgtga tccgccggac cgcgggcgtg gctgtggacg
     4021 gcccggtcga cgtggtccag caggcccacc atcccgatca ctggcgtcgg atccacggcg
     4081 ccagtggggc tctcgttata gaaggagaca ttgccacccg tcaccggcgt gtcgaacgcc
     4141 cggcaggcat ccgtgattcc ccggcacgct tcccggaact gaaagaacac ttccggccgc
     4201 tcgggggacc cgaagttcag gcagtccgtg atccccaggg gccgggcgcc ggtgcaggcg
     4261 atgttcagag ccgcctcggc gaccgcgccc ttgccgcctt cgtacggatc gagggccagg
     4321 taccgggaat tgcagtcggt ggtcacggcg atgccgaaat cggtcccctg aaccctgagg
     4381 acgccggcgt caccaccagg atggatgacg gcacgggcct gaacgctcga gtcatattgc
     4441 tcgtagaccc agcgcttgct cgcgatggag gg
//
LOCUS       DASUBP010000189         4571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_128010, whole genome shotgun sequence.
ACCESSION   DASUBP010000189 DASUBP010000000
VERSION     DASUBP010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4571
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128010"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            158..2647
                     /locus_tag="VFU75_07180"
     CDS             158..2647
                     /locus_tag="VFU75_07180"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5466656.1"
                     /translation="MRSIRLAFRTLFRTPFVTTIAVLSLALGIGANAAIFSLFDQILL
                     RPLPVREPDKLVNLGNPGPKPGSQSCGQAGDCDVVFSYRMFRDLEQRQTVFTGIAAHD
                     RFGVSVVYNNQPLTGDGMYVSGSYFPTLGVRPALGRLFSESDDQSIGTNFVAVLSYAY
                     WQSKLGGDRDVLGKSIIVNGQQLTILGVAAEGFDGTTLGVRPMVFVPISMRGLLTTGF
                     RGFENRRSYWVYLFARLKPGVTIQQASVGINGLYHSIVNDVEAPLQTGMSDATLARFR
                     DKKLELEPGWRGQSSMHKEAKTPLMMLFSVTGIVLLIACANIANLLLARGAGRSMEMG
                     MRLAIGASRWQLISQLLVESVVLASLGGVASLIVAKWTLSGISSLLPSDGNAILQFTL
                     QPAVLLFSAGLSVATGIVFGLFPAWHSTRGELVTTIRANAGHISGARAAARFRATLVT
                     LQISLATALLIAAGLFMKSLVNVTKVDLGVHVDSVITFGLSPEQAGYDSTRALQYYAR
                     VEEALGAIPSVTSVTSSLVPLIAGDNWGNDVKVQGFQSGPDVDANSRFNAIGANYFST
                     FGVQLIGGREFTEADRRGAPQVAIVNQAFAKKFNLGKDAVGKFMSQGGDTLNIQIVGL
                     VPDVKYSDVKKPVPPVFYLPWRQDSHMPFMNFYVRTPQPETVLRTVTGIIRKIDPGVP
                     VEDLKTMPQQIRDNIFLDRMISILSTAFAVLATLLAAIGLYGVLAYSVAQRTREIGVR
                     MALGADAGRVRVMVLRQVGGMMVVGGIIGLVGALGLGKAASSLLYQLKGSDPAVFALS
                     LVVLMLVAFGAGYVPARRASEVDPIKALRYE"
     gene            complement(2730..3995)
                     /locus_tag="VFU75_07185"
     CDS             complement(2730..3995)
                     /locus_tag="VFU75_07185"
                     /inference="COORDINATES: protein motif:HMM:NF024592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEU5466657.1"
                     /translation="MTLTVDLESARNVGVAVLAGLAVGIEREWSGHATGPSARFAGAR
                     TFLLLGLIGGVGGWLATRGFPAFGMVLLAGGSALAVAAYVMAARRSPQDVEGTTEVAA
                     LTVLALGAVAGLGAPLLTSAATSLLLLALAEKTRIHAVIERIGDRELGAALRFAVLAL
                     VVLPLLPAGPFGPYGSIRPRTLWSVVLFLSGITFAGYISRRALGAAAGYGLTGLLGGM
                     VSSTAVILSFANQSRRDPSSSSALARGALGAAAVTIPRVIVILIALDLTLAQAALNSY
                     APMLLLSLGVIAIVLRRPGVPTSTEPPDSRSPLRLGSAIRMALVLQGVLIAVPLAQQT
                     WGATGVLTSAAILGMTSTYALVVAMTTLSQDPGGTTLAMQALAIGITAGMILKLAVGL
                     VAGSGAFRLRLAPGLLILAAAAVLGYYLA"
     gene            4111..>4571
                     /locus_tag="VFU75_07190"
     CDS             4111..>4571
                     /locus_tag="VFU75_07190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466658.1"
                     /translation="MTAMRQRTPEEDTGRAGVDSGPFRWEWRWIGYQSPVQLARLGSS
                     WSRPPTRTAEVYLVSANSPHNVKVREGSIEVKRLRRVSGDGLEQWSPSLRTGFPLLPG
                     DWREVLDALGVPASTPEMPVSSIDALCASLPPGVPVRRIDVIKRRSRLVVGG"
BASE COUNT          745 a         1500 c         1547 g          779 t
ORIGIN      
        1 gtagtccgga acttggggcg tcgtttcaat acggtcgcga gtcggtgcgt gttgcgcaac
       61 cctttcaggg gtccggccat ctcagggaga ggacgcgaaa ctcaggcgta ccgacccccg
      121 tcttcctcta taacctgagc accccgaggc gccgacgatg cgaagtatcc ggttggcatt
      181 ccggaccctc tttcggactc cgttcgtcac caccatcgcc gtcctctcgt tggccctcgg
      241 catcggggcc aacgcggcca tcttttcgct cttcgaccag atcctgctcc ggcccctgcc
      301 cgtgcgggag cccgacaagc tggtcaacct gggcaacccc ggccccaagc cgggctcgca
      361 gtcgtgcggc caggccgggg actgtgatgt ggtcttcagc taccggatgt tccgggacct
      421 cgaacaacgg cagacggtgt tcacggggat cgccgcccac gaccgcttcg gggtgagcgt
      481 cgtctacaac aaccagcccc ttaccggaga cggcatgtac gtctccggtt cgtatttccc
      541 gaccctcggt gtgcggccgg ccctgggccg cctcttctcg gaatccgacg accagagcat
      601 cggcaccaac ttcgtcgcgg tgctctcgta cgcgtactgg cagtccaagc tgggcggcga
      661 tcgggatgtg ctcggcaagt ccatcatcgt gaacggtcag cagctcacca tcctcggcgt
      721 cgcggccgag gggttcgacg ggacgacgct cggcgtgcgc ccgatggtct tcgttcccat
      781 cagcatgcgt gggctcctga ccacgggatt ccgggggttc gagaaccgcc ggagctactg
      841 ggtctatctc ttcgcccgcc tgaaaccggg tgtgacgatc cagcaggcga gtgtcggcat
      901 caacggactc taccactcga tcgtcaacga cgtcgaggcg cctctgcaga cggggatgag
      961 cgacgccacc ttggcgcggt tccgggacaa gaagctggag ctcgaaccgg ggtggcgggg
     1021 ccagagctcc atgcacaagg aagccaagac gcccctcatg atgctgttca gcgtcaccgg
     1081 gatcgttctc ctgatcgcct gcgccaacat cgccaacttg ctcctggcac gcggggcggg
     1141 gcggtcgatg gaaatgggga tgcggctggc gatcggcgcg agccggtggc agctgatttc
     1201 gcagctgctg gtcgaatcgg tcgtcctggc ctccctcggc ggggtggcga gtctgatcgt
     1261 cgccaaatgg acgctgtccg gcatttcgtc gctccttccg tccgatggga acgccattct
     1321 gcagttcacc ctccagcccg ccgtgctgtt gttctccgcc gggctgtcgg tcgcgaccgg
     1381 catcgtgttt gggctcttcc cggcctggca cagcacccga ggcgagttgg tgaccaccat
     1441 ccgggccaac gccgggcaca tttcgggggc gcgggcggct gcgcgcttcc gcgcgacgct
     1501 ggtgacgctg cagatctcgc tcgcgacggc gctgctgatc gcggccggtc tgttcatgaa
     1561 gagtctggtg aacgtgacca aagtggatct gggcgtgcac gtcgacagcg tcatcacctt
     1621 cgggttgtcg ccggagcagg ccgggtacga cagcacgcgg gcgctgcagt actatgcgcg
     1681 cgtcgaggag gccctcggcg cgattcccag cgtgacctcc gtgacctcgt ctctcgtgcc
     1741 cctgattgcc ggcgacaact gggggaatga cgtcaaagtg cagggcttcc agtcgggacc
     1801 ggacgtcgac gcgaactcac gcttcaacgc gatcggtgcc aattacttct cgaccttcgg
     1861 cgtgcagctc atcggggggc gggagttcac cgaggcggac cgccgcgggg ccccgcaggt
     1921 cgcgatcgtg aatcaggcat ttgcgaagaa gttcaacctg gggaaggacg ccgtcggaaa
     1981 gttcatgtcc cagggcgggg acacgctgaa tatccagatc gtcggcctcg taccggatgt
     2041 gaagtactcc gacgtgaaga aaccggtacc cccggtcttc tatctcccgt ggcgacagga
     2101 cagccacatg cccttcatga acttctacgt gcgcaccccg caaccggaga cggtgctgcg
     2161 cacggtgacg gggatcatcc gcaagattga cccgggcgta cccgtggagg atctgaagac
     2221 gatgccccaa cagatccggg acaacatctt cctggatcgc atgatctcga ttctctcgac
     2281 ggctttcgcg gtgctggcga ccctcctggc ggccattggt ctctatgggg tgctggccta
     2341 ctcggtcgcc cagcgcaccc gggaaatcgg cgtccgcatg gcgctcggcg ccgacgcggg
     2401 gcgggtccgg gtcatggtgc ttcggcaggt cgggggcatg atggtggtcg gggggatcat
     2461 cgggctggtc ggggcactgg ggctcgggaa ggcggcgagt tcgctgctgt accagctcaa
     2521 gggatcggac ccggcggtat tcgctctgtc gctggtggtc ttgatgctgg tcgccttcgg
     2581 ggcgggatac gtcccggcgc gccgtgcttc tgaagtagat ccgatcaagg ctctgcggta
     2641 cgagtagcga cccccgcccg gcattcggac gcggggtctg gggggttggg ggcttcgggg
     2701 ctctcacccc tttccttata gatagcgtct caggccaggt agtaaccgag gaccgcggcg
     2761 gccgccagga tgagcagccc tggtgcgagg cggagacgga atgcccccga gccggcgacg
     2821 agtcccaccg cgagtttgag gatcatccct gccgtgatgc cgatcgccag tgcctgcatg
     2881 gccagggtgg tccccccagg atcctgactc agggtcgtca tcgcgaccac cagtgcgtac
     2941 gtgctggtca tgccgagtat ggcggctgat gtgagtacgc cggtggcgcc ccaggtttgc
     3001 tgtgccaagg ggacggcgat caatactccc tgcaggacca gcgccatccg gatggcggaa
     3061 cccagccgca ggggactccg actgtccggc ggctctgtag acgttggtac tcccggacgt
     3121 cgcaggacga tggcgatcac gcccagactg aggagcagca tcggggcata ggagttcaac
     3181 gcggcctgcg ccagggtgag atccagggcg atcaagatga cgatgacccg ggggatcgtc
     3241 accgccgccg cgcccaacgc tccccgcgcc agcgcgctgc tcgaggaggg gtcccggcgg
     3301 ctctggttcg cgaaactcag gatcaccgcg gtcgacgaga ccatgccccc cagcaatccg
     3361 gtcagcccgt aaccggcagc ggcacccaac gcccggcggc tgatgtaccc ggcgaatgta
     3421 atccctgaga gaaagaggac gaccgaccag agggtgcggg ggcggatgct gccatagggg
     3481 ccgaagggac cggcagggag aagcgggagg accaccaggg ccagcaccgc gaaccgaagg
     3541 gccgcgccca gctcccggtc gccgatgcgc tcgatcacgg cgtggattct cgtcttctcc
     3601 gccagcgcga gcagcagcag tgaagtggct gcactggtca acagcggcgc gcccaacccc
     3661 gcgaccgcac cgagcgcgag gacggtgagc gcggccactt cggtggtgcc ctctacgtct
     3721 tgggggctgc gacgggcagc catcacgtag gccgccacgg ccagggcgct gcctcctgcc
     3781 agcagaacca tcccgaatgc cgggaacccc cgggtcgcca gccatccgcc gacgccgccg
     3841 atgaggccca gcaagaggaa cgtgcgcgcg ccggcgaatc gcgcggaagg gccggtggcg
     3901 tgacccgacc actcccgctc gatgcccacc gccagcccgg ccaggaccgc gactcccacg
     3961 ttcctcgccg attcgagatc gaccgtcagc gtcatgctga ttgctagccg gcgctggctg
     4021 aacggaatgt gaagatgtcg tgattcttca gtttcacttc accattgctt cggacggctg
     4081 gggcgagctt cctgcccggg aggaaggctc atgacagcca tgcggcagcg gacacccgaa
     4141 gaggacacgg gaagggccgg cgtcgactcg ggaccatttc gatgggagtg gcgctggatc
     4201 ggctaccaat caccggtcca gctcgcccgg ttgggatcct cgtggagcag gccgccgacg
     4261 cggacggcgg aggtttacct cgtctcggcg aactcccccc acaacgtcaa ggtgcgcgag
     4321 ggctcgatcg aggtgaagcg gctgcgtcgc gtgtccgggg acgggctcga acagtggtcg
     4381 ccctcactgc gcaccggctt ccctctgctc ccaggggatt ggcgcgaggt cctcgacgcg
     4441 ctcggtgtcc ccgcctccac ccccgagatg cccgtgtcga gcatcgacgc gctgtgcgcg
     4501 agtctcccgc ccggcgtacc cgtccggcgc atcgacgtca tcaagcggcg atcccgcctg
     4561 gtggtggggg g
//
LOCUS       DASUBP010000190         3374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_73435, whole genome shotgun sequence.
ACCESSION   DASUBP010000190 DASUBP010000000
VERSION     DASUBP010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3374
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73435"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..646)
                     /gene="mobB"
                     /locus_tag="VFU75_07195"
     CDS             complement(107..646)
                     /gene="mobB"
                     /locus_tag="VFU75_07195"
                     /inference="COORDINATES: protein motif:HMM:NF015181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HEU5466659.1"
                     /translation="MAATRIVSIIGRQNAGKTTLLVALANELRRRKFRVMTIKHATHQ
                     IQLDTEGKDTWRHFQEGHAERVLLEAPGERVLFERTTETGDPVALAEQYLTGADIVLC
                     EGFTSARLPKIEVSRRVAGPPLWKPETAGTGSSGDWIAMVTDDANFRADFPVFRFSDT
                     SWLVTLGNLAWDRARILAP"
     gene            complement(628..1230)
                     /locus_tag="VFU75_07200"
     CDS             complement(628..1230)
                     /locus_tag="VFU75_07200"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HEU5466660.1"
                     /translation="MQGAILAGGQASRLGGRPKGLIQIGGRRILDRVSQLLTEVTGTL
                     PLLIANAPDAGTWLRDVPVVRDRIAGAGALGGIHAALTADGSSPVLCVAWDMPFLTAP
                     FLAALIEGSPPFEAYLPESEGPQGLEPTCAVYRRSCASAIERRIARGDVRAIAFHDDV
                     RVGTLPLARVRTFGDPARLFFNVNSPEDVEQAERAWPPPG"
     gene            complement(1233..2123)
                     /locus_tag="VFU75_07205"
     CDS             complement(1233..2123)
                     /locus_tag="VFU75_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466661.1"
                     /translation="MTRLASVLCAFALVSAPVALHAQDSESGQTNLVLSVFGGINAGH
                     SLWNIARQPLCVLQGSGGNYNGCQQYKTATDSGNLSDTLSFSRDVSSSILLGAAVSIF
                     PNSHVGFHGEIYYFGLTYDDRCANVGAPYQSDPENKNQQLCNDFASSTASASAVSFVG
                     GVTLRASPHHAISPYVRAGVGVTTYSGGTLSVSGNFATNATDPATGIPIVVSRAVVVD
                     TAPKTTSWTFQMGVGFTARVSPGYQFRMELQDALVPLERLIGPANDFGQAPHDTKVYH
                     HIGLIIGLDVVLEHRRGHRY"
     gene            complement(2120..2530)
                     /locus_tag="VFU75_07210"
     CDS             complement(2120..2530)
                     /locus_tag="VFU75_07210"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEU5466662.1"
                     /translation="MTEAELARLVREAKTIAVVGLSPKTARPSHEVATYMQEAGYRIV
                     PVNPGHDRILGERSYRTLADAARDQRIDIVDVFRRSALAGGVVDEALAIRPGLIWLQV
                     GVVDEEAAARAAAGGIPMVMDRCLAVYHQQLEAR"
     gene            complement(2527..2985)
                     /locus_tag="VFU75_07215"
     CDS             complement(2527..2985)
                     /locus_tag="VFU75_07215"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HEU5466663.1"
                     /translation="MELLSPDRGGTPTVRLTRIVEFHAGHEIRRADWTPEQNAEHFGR
                     ASQLHSHTYQCHVTVSGALHPTAGGVVRLAELDALLKREVTDRFEGKTVHEGAPEFGP
                     GGWLSTGEALSVYLWGRISGALPPGVRLHAIRVQEGPQLYSEYFGESAGG"
     gene            complement(2973..>3374)
                     /locus_tag="VFU75_07220"
     CDS             complement(2973..>3374)
                     /locus_tag="VFU75_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466664.1"
                     /translation="AVKNNTSGLIDADVVLVDATLQMALTGPKTWRGLAPYIGASLGV
                     AAGRDTPADSSGDKFGTKFTFAPVIGAKYYVGRRVALSADFRAVFWRLGYPSQFKVAD
                     KVDNIPILSIDAADNNWTVHPWISLGLGWSF"
BASE COUNT          540 a         1104 c         1189 g          541 t
ORIGIN      
        1 tcggcgagga cgagtccccg gcagtcggcg agaggccggc gcacggggtt gagcggacgg
       61 acttgctcga ggatgaggcg ggccgcggct tccgccgcca gcgcggtcac ggcgcgagaa
      121 tccgcgcccg gtcccaggca aggttgccga gggtcacgag ccaggaggtg tcactgaacc
      181 ggaagaccgg gaaatccgcg cggaaattcg cgtcgtccgt caccatcgcg atccagtcgc
      241 cggagctccc cgtcccggcc gtctcgggct tccacagggg cgggccggcg acgcgccgcg
      301 agacttcgat cttgggcagg cgggcgctcg tgaatccctc acagagcacg atgtcggcac
      361 ccgtcagata ctgctcggcg agcgcgaccg gatccccggt ctcggtcgtg cgctcgaaga
      421 gtacccgttc cccgggggcc tcgagcagga cgcgctcggc gtggccctcc tgaaagtgcc
      481 gccaggtgtc cttgccttcg gtgtcgagct gaatctgatg ggtcgcgtgc ttgatggtca
      541 tcacgcggaa cttccggcgc cggagctcgt tcgcgagcgc caccaggagc gtggtcttcc
      601 cggcgttctg gcgtccgatg atggaaacta tccgggtggc ggccacgcgc gttccgcttg
      661 ctccacgtct tcgggggaat tgacgttgaa aaagaggcgc gccgggtcgc cgaaggtccg
      721 cacccgcgcg agcggtagtg taccgacccg cacgtcatcg tggaaggcga tggcgcggac
      781 gtcgcctcgg gcgatgcggc gttcgatggc ggaagcgcag gagcgccggt agacggcgca
      841 cgtcggttcc agaccctgcg gtccttcact ctccgggagg taggcctcga aggggggtga
      901 cccttcgatg agagcggcaa gaaatggtgc ggtgagaaaa ggcatgtccc acgcgacgca
      961 gaggacgggg gaggatccgt cggcggtcag ggcggcgtgg atcccgccca gggcgccggc
     1021 cccggcaatt cggtccctga cgaccggcac atcccgcagc cacgtgccgg catcgggggc
     1081 gttggcgatc agcaggggaa gggtgccggt gacttcagtg aggagctggg agacgcggtc
     1141 gaggatccgc cgtccgccga tttggatcag gcccttgggg cgacctccga gtcgcgaggc
     1201 ctgccccccg gccaggatgg cgccctgcac gatcagtagc ggtgccctcg gcggtgctcg
     1261 agcacgacgt ccaaaccgat gatcaggcca atgtggtggt agaccttggt gtcgtggggt
     1321 gcctggccga agtcgttggc gggtccgatg agacgctcga gcggcaccag ggcgtcctgg
     1381 agctccatgc ggaactggta tccggggctg acccgtgcgg tgaagcccac ccccatctgg
     1441 aacgtccacg acgtggtctt gggggcggtg tcgacgacca ccgcccggct caccacaatc
     1501 ggtattccgg tcgccggatc ggtggcgttg gtggcgaagt tgcccgacac cgacagcgtc
     1561 cctccgctgt aggtcgtcac gcccacgccc gcgcggacat acgggctgat cgcgtgatgc
     1621 ggcgacgctc ggagtgtgac gcccccgacg aacgacaccg cggaggcgga cgcggtggag
     1681 ctggcgaagt cgttgcagag ctgctggttc ttgttctcgg ggtcactctg gtacggcgcg
     1741 ccgacgttgg cacagcggtc gtcgtacgtg agaccgaagt agtagatctc tccgtggaac
     1801 ccgacgtggg agttcggaaa gatgctcacc gcggcgccca gcaggatgct cgacgacaca
     1861 tcgcgtgaga aggacagggt gtccgacagg ttccccgagt cggtggcggt cttgtattgc
     1921 tggcagccgt tgtagttccc gccgctgccc tggagcacgc agagcggttg ccgtgcgatg
     1981 ttccacaggg agtgcccggc gttgatgccg ccgaacacgg aaagcaccag gttggtctgc
     2041 ccgctttcgc tgtcttgcgc gtggagcgcg acgggcgcgg acacgagggc gaaggcgcac
     2101 aggacggacg ctagacgggt catcgagcct cgagctgttg gtggtacacg gcgagacagc
     2161 ggtccatcac catcggaatc cctccggcgg cggcgcgagc cgcggcctcc tcgtccacca
     2221 cacctacctg caaccagatc agtcccgggc gaatcgcgag ggcttcatcc accacgccgc
     2281 ccgccagcgc gctccgccga aatacgtcca cgatgtcgat gcgctgatcc cgggccgcgt
     2341 cggcgagcgt ccggtagctg cgctcgccca gaatccggtc gtggccgggg ttcaccggaa
     2401 cgatccggta tcctgcctcc tgcatgtacg tcgcgacttc atggctcggc cgcgcggtct
     2461 tcggagacag tccgaccacc gcgatggtct tcgcttcccg gacgagacgc gccagctccg
     2521 cctccgtcaa ccgcccgccg attccccgaa gtactcggaa tacagctgag gaccctcctg
     2581 cacccggatc gcatgcagcc ggaccccggg cggcaacgcc cccgaaatcc gcccccacag
     2641 atacaccgac aaggcctcgc cggtcgacag ccacccgccg ggcccgaatt ccggggctcc
     2701 ctcgtggacg gtcttcccct cgaatcggtc ggtgacctcc cgcttgagaa gggcgtccag
     2761 ctccgccagg cgcacgaccc ctcccgcggt ggggtgcaga gcgccggaaa cggtcacgtg
     2821 gcattgatag gtatgggaat gcagctggga ggcgcggccg aagtgctcgg cattctgctc
     2881 gggtgtccag tcggcccggc gaatctcgtg gccggcgtgg aattcgacga tccgggtcag
     2941 gcggacggtc ggggttcccc cacgatccgg actcagaagc tccaccccag gccgaggctg
     3001 atccacgggt gcacggtcca gttgttgtcg gctgcgtcga tcgagaggat cggaatgttg
     3061 tcgaccttgt ccgccacctt gaattgggac ggatacccca ggcgccagaa gaccgcgcgg
     3121 aagtccgcgg acagcgcgac cctccgcccg acgtagtact tggcaccgat taccggagcg
     3181 aaggtgaact tggtgccgaa cttgtcgcct gagctgtcgg cgggcgtgtc ccggcccgcc
     3241 gcgacgccga gggaagcgcc gatgtagggg gcgaggcctc gccacgtctt gggtccggtg
     3301 agcgccattt gcagcgtggc gtccacgagc accacgtcgg cgtcgatgag ccccgacgtg
     3361 ttgttcttga cggc
//
LOCUS       DASUBP010000191         2160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_169212, whole genome shotgun sequence.
ACCESSION   DASUBP010000191 DASUBP010000000
VERSION     DASUBP010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2160
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169212"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="VFU75_07225"
     CDS             <1..732
                     /locus_tag="VFU75_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466665.1"
                     /translation="ALAVIDYPHIEPRGVVHPSVVEIKVAPIALPALSRIGYIRGASD
                     RVPEALTAVGLPVEVLSADTLAKGDLSRYDAIVIGSRAYETDPALVANNGRLLDYARN
                     GGLIVVQYQQYPFIQGGFAPYPMTIARPHDRVTDENAAVTVVDSLSRVLKSPNPIGPS
                     DWQGWVQERGLYYAHDWDSTYHPVFTMHDPGDPPLAGGLLIAPLGRGTYVYTGLSFFR
                     ELPAGVVGAYRLFANLLALKPNSTP"
     gene            729..1838
                     /locus_tag="VFU75_07230"
     CDS             729..1838
                     /locus_tag="VFU75_07230"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEU5466666.1"
                     /translation="MSREPGAGHSTQGRGPAASRSVRLPAYGSRLFVGAIMLALWGCS
                     GGGRTPLVIYSPHGRDLLTLLQKRFEQLHPDIAVHWLDMGSQEVYDRVRSERANPQAD
                     VWFGGPATIFARGAQDSLLQPFRPTWAGTVAPHERGPGDLYFASYETPAVIVYADQAM
                     KPAEAPQTWDALLEPKWKGKIIIRDPLASGTMRAVWGLLIERGFKKSADTAVAFGYLK
                     ALDAQTKEYVLNGTLLDQKIVRQEALITIWDLPDILLNKREGMQLGYVFPRDGTPVIE
                     DAIGIVKGAKHAAAAKLWEEWIGSTEAQLLATRDGYRLPARQDLPADSLPAWAQEVRR
                     QMVTATDMDWDQLATHGADWMRYWDERVRARGNRP"
     gene            1835..>2160
                     /locus_tag="VFU75_07235"
     CDS             1835..>2160
                     /locus_tag="VFU75_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016449231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5466667.1"
                     /translation="MSFLSVDNLQVRFSDTVTVGPVSLDIERGEILALLGPSGSGKTT
                     LLRMLAGFETPDSGRVLVENEDVTGMAPAARRFGMVFQHYALFPHLNVGENVAFGLAR
                     GSDRAA"
BASE COUNT          347 a          705 c          746 g          362 t
ORIGIN      
        1 gctctggccg tcatcgacta cccgcatatc gagccgaggg gcgtggtgca tccgtcggtg
       61 gtggagatca aggtggcgcc gatcgcgctg ccggcacttt cccgcatcgg ctacatccgc
      121 ggcgcatccg accgggtgcc cgaggcgctc accgccgtcg gtctgccggt cgaggtactc
      181 agcgccgaca ccctggcgaa gggcgatctc tcgcgctatg acgccatcgt gatcggcagc
      241 cgggcgtatg agaccgaccc ggcgctcgtg gcgaacaatg ggcggctgct ggactacgcg
      301 cgcaatggcg ggctgatcgt cgtgcaatac cagcagtacc cgttcatcca gggcggcttc
      361 gccccgtatc cgatgacgat cgccaggccc catgatcgcg tcacggacga gaatgcggcg
      421 gtgaccgtgg tggattcgct gtcacgcgtg ctcaagagcc cgaatccgat cgggccgtcg
      481 gactggcagg ggtgggtgca ggagcgcggg ctgtactacg cgcacgactg ggattccacg
      541 tatcacccgg tgttcaccat gcacgacccg ggggacccgc cgctggccgg tggcctcctc
      601 atcgctcccc tcggccgcgg gacgtacgtc tacacgggac tgtccttctt ccgtgagctt
      661 cccgccgggg tcgtcggggc gtatcggttg ttcgcgaatc tcctcgcatt gaagcccaat
      721 tcgaccccgt gagccgggag ccgggagccg gacactccac ccaggggcgg ggtcccgcgg
      781 cgtcacggag cgtacggctc ccggcgtacg gctcccgtct gttcgtgggc gccatcatgc
      841 tggccctgtg ggggtgttcc ggggggggaa gaactcccct ggtgatctac tcgccccacg
      901 gccgcgacct cctcaccctg ctgcaaaaac gcttcgagca actgcacccc gacatcgccg
      961 tccactggct cgacatgggc tcgcaggagg tctacgaccg cgtgcggtcc gaacgcgcga
     1021 atccccaggc cgacgtctgg ttcggggggc cggcgacgat cttcgctcgc ggcgcacagg
     1081 actcgctcct ccagcccttt cgtcccacct gggccggcac ggtcgcgccg cacgagcgcg
     1141 gtccgggtga tctctacttc gcgtcgtacg agacgccggc ggtgatcgtc tacgccgatc
     1201 aggcgatgaa gcccgccgag gcgcctcaga cgtgggacgc gctcctcgag ccgaagtgga
     1261 aagggaagat catcatccgt gatccactgg cgtccggtac gatgcgggcg gtgtggggcc
     1321 tcctgatcga gcggggattc aagaagagcg ccgacacggc ggtggccttc ggctatctca
     1381 aggccctcga tgcgcagacc aaggagtacg tcttgaacgg aaccctcctc gaccagaaaa
     1441 tcgtgcggca ggaggcactg atcaccattt gggacctgcc ggatattctg ctcaacaagc
     1501 gggaaggcat gcagctgggc tacgtctttc ccagggacgg gactccggtg atcgaagacg
     1561 cgatcgggat cgtcaaaggc gccaagcacg cagcggccgc gaagctgtgg gaggaatgga
     1621 tcgggtcgac cgaggcgcaa ctcctcgcga cgcgcgatgg ctaccggctc cccgctcgcc
     1681 aggacttgcc ggccgactcg ctgccggcgt gggcgcagga ggtccggcgt cagatggtga
     1741 cggccaccga catggattgg gaccagctcg ccacgcatgg cgcggactgg atgcgttact
     1801 gggacgaaag agtccgcgcc cgcggcaacc gtccgtgagt tttctctccg tcgacaactt
     1861 gcaggtccgg ttcagcgaca cggtgacggt cggtcccgtg tccctggaca tcgagcgcgg
     1921 ggaaattctc gcgctgctgg ggccgtcggg cagcggcaag accaccctgc tgcggatgct
     1981 ggcgggcttt gaaacgcccg actcgggccg ggtgctggtg gagaatgaag acgtgacggg
     2041 gatggcgccg gcggcgcgac ggttcggcat ggtgtttcag cactacgcgc tcttccctca
     2101 tctgaatgtc ggggagaacg tcgcgttcgg tctggccagg ggcagtgacc gcgcggcgca
//
LOCUS       DASUBP010000192         4515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_100677, whole genome shotgun sequence.
ACCESSION   DASUBP010000192 DASUBP010000000
VERSION     DASUBP010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4515
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100677"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1320)
                     /locus_tag="VFU75_07240"
     CDS             complement(145..1320)
                     /locus_tag="VFU75_07240"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5466668.1"
                     /translation="MRIAILTHNYPRFAGDPAGAYVARLATSAAGAGHDVHVLAPHVT
                     GTKEEERVSGGPEVRRFRYAPEALERVGYRGDVRSRTLFSPLTLLLLPFFLAAFRRAA
                     RRLVQEVRPEVVHAHWWFPGGWIASGLPVPYVVTSHGSDARLLDRGPWFRGVALPVFR
                     RAARVTAASRFLATDLERRVPGPSVVVTPMPVDVERFGAAFKTPRVVPPRILYAGNLV
                     VGKGVDVLLRAVAELKQRSGPFRVKILGEGPELPPLKALAGQLGIAADVDWSPFVTQD
                     RMPAEYGAATVTVLPSRGQAEGLGLTLVEALLAGSAVVGTPAGGIPEVIEDEKTGLLA
                     RDGDANHLAGQIERLLRDGELRRRLIAEGASRMRETYAPQAAAERFLRIYAEIARPA"
     gene            complement(1396..2613)
                     /locus_tag="VFU75_07245"
     CDS             complement(1396..2613)
                     /locus_tag="VFU75_07245"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU5466669.1"
                     /translation="MALGDRFAALVFCAALAACSNPVQPERGGAKTGAAGIRVSVVLA
                     RVGVGQSFQLFVTARDSNGAVIPNPTVTYASSNTGLATVSSGGLIHGVSLGPTDVRVT
                     AGGATAVVPVVVATSPAGTLTGTLSGADSVASRPFTVTISSKNDVYVGRQDLPYVQRT
                     QLPDTTFGDSVLVGADPTDITFDFAGTTAYITDQFSSSLGVAPVTAMSLEDSIPLPGQ
                     PYRVVLDLEGNTAFVTTNNQLVVAVNLLTRDTSRTWSLDNPSNGLAWSPDGVHIYAST
                     YGGYLHSLNTSTGTDSTILLSGKLQDVVVANDGRTVYVANETGAMWVLDSTGAHQAPG
                     VQNTFSLVLSPDGNSLYASQPALGTVLIVDRASLTVSKTLDVGGMPRRIAFDPLGTVA
                     LIGNEGGYVSIVR"
     gene            2920..4335
                     /locus_tag="VFU75_07250"
     CDS             2920..4335
                     /locus_tag="VFU75_07250"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HEU5466670.1"
                     /translation="MPALSLDVILMALLPWLSAQGRAALRATVAAGGELDADALARNM
                     GLTNRFHLNRLLKHEGLPAFGVLSDWISILALTLESEATGASLLHLAATRQLEPATCY
                     RRFRRTLGLSWRESRERGIGWMLLKFRDRCARPSITPVVGAHRGPQSAFERRAPALIG
                     LGRQGPRLVRESPAAARLARRGHPAGRISATVPLAHGGFDVAVEPAGFALITRAATAT
                     VERLHLRSLRFEEPFHVGYNPTRVAICDSGLAYVSNQFSGSLSIVDGPGRRVIGEIPV
                     TGDPAPVLPASDGRTVFVATNADRLYAIQADSGAIQASLPLPSTSHQLARHPSRPLLY
                     VATRDAGTVLEVNQTDLTQVREFVVGGRTQGLVVAPDGTELYVANEQGGLDVIDLATG
                     SLTASLELGGPAFSVVATPDWSQLYVGLVRAERVQVLDRGTLRVVHTIPTGGVPRGIA
                     FDPRTQTGLIANEAGWITVVA"
BASE COUNT          766 a         1575 c         1472 g          702 t
ORIGIN      
        1 ctccctcact caccgtcagc cagtgaacgc ggtagtgaag gcagcgcgca atcccggtaa
       61 tccaacccgg gtggtgatag aagttgccga gctccccggc gagcgtgtcc caccccgccg
      121 ggatgtccgc atgacgaatg acgttcacgc gggcctcgcg atctcggcgt agatccggag
      181 aaaacgctcc gcggccgcct gcggcgcgta ggtctcacgc atgcgtgacg caccttcagc
      241 gatcagccgg cggcgcagct ccccgtcgcg caggagacgc tcgatttgtc ccgcgaggtg
      301 attcgcatcg ccatccctgg cgagcaaacc cgtcttttcg tcctcgatca cctccgggat
      361 cccacccgcc ggggttccga ccacggcaga gcccgcaagt aacgcctcga ccagcgtgag
      421 acccagccct tctgcctgtc cgcgactcgg caggacggtg accgtcgccg cgccatactc
      481 ggcgggcatc cggtcctgcg tcacgaacgg agaccaatca acgtcggcgg cgatgccgag
      541 ctgtccggcg agcgccttga gcggcggcaa ttcgggtccc tcgccgagaa tcttcacgcg
      601 gaaggggccc gagcgctgct tcagctcggc gacggcccgc aggaggacgt cgaccccctt
      661 ccccaccacc aggttcccgg cgtagaggat gcggggcgga actacgcgcg gcgtcttgaa
      721 cgccgcgccg aagcgctcca catccaccgg catcggggtc acaaccaccg agggtccggg
      781 cacccgccgc tccagatcgg tggccaggaa ccgggacgcc gccgtcaccc gcgcggctct
      841 ccgaaacacc ggaagcgcca caccgcgaaa ccagggcccg cgatcgagca gtcgggcgtc
      901 cgagccatgg gaagtcacga cgtaggggac cgggagaccg gaggcgatcc agccgccggg
      961 aaaccaccag tgcgcatgca ccacctcggg ccggacctcc tgcaccagtc gccgggcggc
     1021 ccgccgaaac gcggccagaa aaaatggcaa cagcaggagg gtgagggggg aaaacagggt
     1081 ccgggagcgc acatccccgc ggtagccgac ccgctccagc gcctctggcg cgtaccggaa
     1141 ccgccggacc tcgggtcccc cactgacccg ttcttcctct ttcgtgccgg tgacgtgcgg
     1201 agccagcacg tgcacgtcgt gccccgcccc ggcggccgag gtggccagtc gggcgacgta
     1261 ggcgcctgcc ggatctccgg caaagcgggg gtagttgtgg gtcaggatgg cgatgcgcac
     1321 cggtgctcaa agctacgaaa cgcccccggc taccgaaatg ggccgggggc gtccaacctg
     1381 cggcaggaaa gcgatttacc gcacgatact cacgtacccg ccctcattcc cgatcagggc
     1441 caccgtcccg agcggatcga aggcgatccg gcggggcatg ccgccgacgt ccagtgtctt
     1501 gctcaccgtc agggacgccc gatcgacgat gagcaccgtc cccagggccg gctgcgaggc
     1561 gtagagagag ttcccatcgg gtgacagcac gagactgaag gtgttctgca ctcccggggc
     1621 ttggtgcgcc cccgtcgaat cgagcaccca catggcaccg gtctcgttcg cgacgtacac
     1681 ggtgcgccca tcgttcgcaa cgacgacgtc ctggagcttg ccgctgagca ggatggtgga
     1741 gtcggtccct gtgcttgtgt tcaaggagtg cagatagccg ccgtacgtcg acgcgtagat
     1801 gtgcacgccg tccggggacc aggcaagtcc attggaggga ttgtcgaggg accacgtccg
     1861 ggacgtatcc cgcgtcagca gattcaccgc gaccaccagc tggttattcg tcgtgacgaa
     1921 tgcggtattc ccctccagat cgagaacgac tcggtagggc tggcccggca agggaatact
     1981 gtcttccaac gacatcgccg tgaccggcgc gacgcccaga gagctggaga actggtccgt
     2041 gatataagcg gtggtgccgg cgaagtcgaa cgtgatatcg gtcggatcgg cgccgacgag
     2101 caccgaatca ccgaacgtcg tatcgggcag ctgcgtccgc tggacatacg gcaggtcctg
     2161 acggccgacg tagacgtcgt tcttcgagga gatcgtcacc gtgaatgggc gtgacgcgac
     2221 cgagtccgcg ccgctcagcg ttcccgtcag ggtccccgcg ggactggtcg ccaccaccac
     2281 tggcaccact gccgtcgccc ctccggccgt cacgcggacg tcggtcgggc ccagagagac
     2341 gccgtgaata agcccgccac tggagacggt cgcgagaccc gtgttggacg aagcgtaggt
     2401 caccgtggga ttgggaatga cagcgccgtt cgagtcacgg gcagttacaa aaagctggaa
     2461 agattgtcct actcccaccc gggcgagcac cacgctgacc ctgatgccgg cggcacccgt
     2521 cttggcgccc ccacgttctg gctgcactgg attcgagcag gccgctaacg cggcgcagaa
     2581 cacgagtgct gcgaatctgt ctccgagggc catgacggtc tccctgtctg cggtttcctg
     2641 cggtcatccc aagagacagc atcgaatgtc gccggtcgcg cgacgcccgt ctcattcgga
     2701 ataataggtg cacattgcgc acaccgcgat gtcgtttggt gcggagtcgc ccgagcgtgg
     2761 cgtggcgttt cgtatcgtcg acgcatcggc aatcgccacc gcacgatgtt tcattatgca
     2821 ccaccatccg cgcgtattct ggggtaggaa cacgccggtc ccaactgttg cggctatggc
     2881 gttcggtgcg gccccgtcac ccatcgagtc ggagtccaga tgcccgcgct atcgctcgac
     2941 gtcattctca tggcgctcct gccttggttg tccgcccagg ggcgcgctgc gttgcgggcc
     3001 acggtggccg cgggcggcga gctcgatgcc gacgccctcg cccgcaacat gggtctcacc
     3061 aaccgctttc acctgaatcg cctcctcaag cacgaagggt tgccggcctt cggcgtactg
     3121 agcgactgga tttcgattct cgccctgacg ctcgagtccg aggcaacggg cgcctccctg
     3181 ctccatttgg cggcgacgcg gcagctcgaa cccgccactt gttaccgccg cttccgccgc
     3241 accctcggtc tctcctggcg ggaatcgcgt gagcgcggga tcggctggat gctgctcaag
     3301 ttccgcgacc ggtgcgcccg cccctcgatt accccggtcg tcggcgccca ccgcggccca
     3361 caaagcgcct tcgagcgccg cgccccggcc ctgatcggac tgggccgtca gggaccccgc
     3421 ctggtgcggg agtctccggc agccgcccgg ctcgcgcggc gcggccaccc ggcagggcgg
     3481 atttccgcga ctgtccccct cgcacatggc ggattcgacg tcgccgtgga acccgccgga
     3541 ttcgccctga tcacccgggc ggcgaccgcc acggtcgagc ggctccacct ccgctcgctg
     3601 agattcgagg aacccttcca cgtcggatac aacccgacgc gcgtcgcgat atgcgactcc
     3661 ggactggcgt acgtgtccaa tcagttcagc ggctcgctct ccatcgtcga tgggccgggg
     3721 cgacgcgtga tcggcgagat tcccgtcacc ggcgatccgg cgccggtgct cccggcgtcg
     3781 gacgggcgca cggtgttcgt cgccaccaat gcggaccgct tgtacgccat ccaggccgac
     3841 agcggggcca tccaggcgtc gctgcccctg ccctcgacct cgcatcaatt ggcgcgccac
     3901 ccgagccgcc cgcttctgta cgtcgcgacc cgggatgcgg gaaccgtcct cgaagtgaat
     3961 cagacggatc tcacgcaagt gcgggagttc gtggtcggcg ggcggacaca gggactggtc
     4021 gtagctccgg acggaaccga gctctacgtg gcgaacgaac agggtgggct ggatgtcatc
     4081 gatctggcga ccggctcgct gaccgcgtca ctcgagctgg ggggcccggc attctcggtg
     4141 gtggcgaccc cggactggag ccagctctac gtcggactgg tgcgcgcgga gcgggtgcag
     4201 gtcctggacc gtgggaccct gcgggtggta cacaccattc cgacgggtgg cgtgccgcgc
     4261 gggatcgcgt tcgacccccg cacgcagacg ggcttgatcg cgaacgaagc aggatggatt
     4321 acggtcgtcg cctgaccgga gacctcaccg cccggcgttc gaccggtcgc cgagggcccg
     4381 gagctcctcc cgcatccccg ccaccagctc tcccacgaac ccgaagccga agagcacgag
     4441 gccggcgagc accaggagca tcacgagata caaccacggg cggaaaccga tcccgaatac
     4501 gtaacgctcg atgat
//
LOCUS       DASUBP010000193         7409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_128191, whole genome shotgun sequence.
ACCESSION   DASUBP010000193 DASUBP010000000
VERSION     DASUBP010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7409
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128191"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..301
                     /locus_tag="VFU75_07255"
     CDS             <1..301
                     /locus_tag="VFU75_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEU5466671.1"
                     /translation="NPDICNIFTLHQGFSSAPVQQDVAQKCRSAGWGCLDCKRVLADN
                     MIAALAPIRERAAELRAHPDRVREMLSAGAHKAQKIAAKTLADVRSAMGFMPPGA"
     gene            298..1323
                     /locus_tag="VFU75_07260"
     CDS             298..1323
                     /locus_tag="VFU75_07260"
                     /inference="COORDINATES: protein motif:HMM:NF014841.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HEU5466672.1"
                     /translation="MSRRLVVDLSAVRAVWRIPPHCVEQIRAALPGWDVVLVDAPANS
                     DGDGGGGSPAAIAAARGAEVYLGFGLPGGVVETSRGTLKWVHTATAGVSPALAALKGS
                     GVTVTNSRGIHAEPMADWVLGAVLHFARGFDYAVRAQAQSRWTKDAFTDGSTAFPEVR
                     DLRLGIFGLGGIGEAVAKRGLALGMKVMGIRRRPGQGGPTGVKVVGGFEALGTLALET
                     DILAITVPRTPETAGTVDAGLLGKLPRGAVVINVSRGGILNEDALLAALDSGHLRGAA
                     LDVFATEPLPPEHPLWRHPRVLMTPHVSAVTARFWDREMALILDNVRRYLGGHRLVNV
                     VDLEAGY"
     gene            1323..2519
                     /locus_tag="VFU75_07265"
     CDS             1323..2519
                     /locus_tag="VFU75_07265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HEU5466673.1"
                     /translation="MAEASKEKAPQPQPQGSGFERIIKAAVDRGASDLHIKAGDVFRA
                     RIDGKLVPLTKQRLTPDQTRAIALQLIPSEEDRARVDKVRDYDCSWGMPGIGRFRVNI
                     LRQRSSFMIVMRVIPFDVPSFESLKLPPALNQVASAERGMILVTGVTGSGKSSTMAAI
                     INHINQNQHKHILTLENPIEFLHRDVNCSVTQREIGTDTDDFRMGLRAALRQDPDVVL
                     IGEMRDAETIDTAMKAAETGHLLISTLHTPDAVTTISRIVAMFPPEEQDICRIRLADN
                     IQAVVSQRLLPRADGKGRAAALEILIATGTVRDMIRDPNRTPELRDYMRESREQYGMQ
                     TFDQHLMDLVAEGTVTYETAIATSSNPADFELQMRTLRRRSRTVQAGDVVPEGFTDGI
                     SGLSPQ"
     gene            2516..3391
                     /locus_tag="VFU75_07270"
     CDS             2516..3391
                     /locus_tag="VFU75_07270"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU5466674.1"
                     /translation="MTLTRVTGILAPVTTPFGMSGEIDTAALQQNIAKGLADGLSGVV
                     IAGSTGEAPLLEREEQRRLVFAARETVPADRVLLVGTGAESTRAAVALCKDAGAEHAD
                     AVLVRPPGYYAPMLDARAVLDHLRAIADASPVPVLLYNIPKYAPVAITLEMLAKMQEH
                     PNVIGIKDSSGDPANLAAYRTAVPKWSVLVGSASHLKRAIELKCEGGVLGAACFATAL
                     CVALKKKAELGDPEAEPLQVRVATVDKEIVGRLGPAGIKVAMDRVGLIGGNPRPPLRP
                     LTEGERDRVAALVAA"
     gene            3388..4431
                     /locus_tag="VFU75_07275"
     CDS             3388..4431
                     /locus_tag="VFU75_07275"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU5466675.1"
                     /translation="MTPGLGGPELLSRIAIPRATGTEGNRAVRELLTEALQARGFEVE
                     TQEFAAGPDALVAVQLAGALLGGGAFAALLLSQIPGWHGLLPVFALALIAGIGIYLQR
                     ASNKPPARRATGVNLIARKGTGLPTAWLVAHYDSKSQPLSMAGRIIAGGLSVAGAAGV
                     LVFAAAPSPMAAILLLPAMLGGAMLARCRVENRSPGALDNGSALVAIFEILDRAPNAR
                     IGVLLPDAEEWGLLGARAAARERPDLFGGRPVINLDGLDDRGAPILFVHRPGALVERL
                     RSQLRARVARRLPVFVDGIALAPVAGECLTIMKGNWASARIVHTPRDVPGSLKLTGVK
                     DVAAGVAAVLGGR"
     gene            4510..5790
                     /locus_tag="VFU75_07280"
     CDS             4510..5790
                     /locus_tag="VFU75_07280"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEU5466676.1"
                     /translation="MFGPKSHCVVVVGAQWGDEGKGKIVDVLAERADLVVRYQGGANA
                     GHTVVTGTEEFILQQVPSGVLQGAVCIVGNGVALDPDTLFMELDALAKRGVQWDHKVL
                     VSDRAHLTLPYHKLLDRAREKQEKIGTTGRGIGPTYEDKYGRRGIRVGDLRNLERAKK
                     LVEERVAGANQVLGMLGAADRADPKEHCALVDRLAPRLLPITVDAGRIVWDALGRGES
                     VLLEGAQGALLDVDHGTYPYVTSSNTTAGGAAVGAGIGPTAIDAVLGVVKAYTTRVGN
                     GPLPTEATDALSERIRELGGEYGAVTGRPRRCGWFDAVVVRYAVRVNGLTGLAVTKLD
                     VLDTFAEIPVCVGYKLDGEVFDSMPADLERIERVEPIYETLPGWQSPLGEARRLADVP
                     PAARAYLDRLQDLAHAPIRYVSVGTRRDQIIEVG"
     gene            5808..5984
                     /locus_tag="VFU75_07285"
     CDS             5808..5984
                     /locus_tag="VFU75_07285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466677.1"
                     /translation="MGKSDEQQMAKLVKKLSQQAQKPKKNLRRQGDPTLPPETTDADM
                     ERQDFFKEMKKREF"
     gene            6246..7325
                     /locus_tag="VFU75_07290"
     CDS             6246..7325
                     /locus_tag="VFU75_07290"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR00045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEU5466678.1"
                     /translation="MPAILVAPAAFKGTYGPRQVADAIVAGVRRAIPNAKVLACPVAD
                     GGDGLLEAVLPPGSLLERVTVTGPLGAPVAAELGWLDAETAIFASSSACGLALIPPGQ
                     RAPLIATTRGVGELIWEAADRGAKTVVVGLGGSATVDGGTGAARGLGWSFVDATGAEL
                     ADGGGALGDLAVISGGWRLGAQVVALTDVTTPLAGPAGAAVIFGPQKGASDSEVERLS
                     AGLSRLAECLAQAGHPGLDVLPGGGAAGGLGAGLAAFARAALTPGAAWVLDRIGFDAA
                     LATVDLVITGEGEFDATSFAGKVTGEIVRRAQAARRRVAVVAGKASGQVGITVVSGNG
                     STLSPAEIEGLGERAAREALGLPAS"
BASE COUNT         1197 a         2385 c         2617 g         1210 t
ORIGIN      
        1 gaacccggat atctgcaaca ttttcaccct gcatcagggt ttttcgtccg ctccagtcca
       61 gcaggacgtc gcccagaaat gccggagcgc gggctggggc tgcctcgact gcaagcgggt
      121 gctggcggac aacatgatag ccgcgctggc tcccatccgc gaacgtgccg ccgagctgcg
      181 cgcacatcct gaccgggtgc gcgagatgct ctccgcgggc gcgcacaagg cgcagaagat
      241 cgccgcgaag acgctcgccg acgtgcgatc ggcgatgggc ttcatgcccc cgggagcgtg
      301 agccgccgcc tggtggtgga cctctccgcc gtgcgagcgg tgtggcgcat tccccctcat
      361 tgtgtcgagc agatccgcgc ggcgctcccg ggttgggacg tggtgctggt ggacgctccc
      421 gccaactccg acggcgatgg cggaggtggg tcgccggccg ccatcgccgc agccaggggc
      481 gccgaggttt atctggggtt tgggctcccg ggcggcgtcg tcgagacgtc tcgcggcacc
      541 ctcaaatggg tccacaccgc caccgccggc gtatcgcccg ccttggcagc actcaaaggc
      601 tccggcgtca ccgtgaccaa ttcccgaggg atccatgccg agccgatggc ggactgggta
      661 ctcggcgccg ttctgcattt cgcccgcgga ttcgattacg ccgtacgcgc ccaggcgcaa
      721 tcgcggtgga ccaaggacgc tttcacggac gggtccacgg cgttccctga ggtcagagat
      781 ctccgactcg ggatcttcgg ccttggcggg atcggggaag ccgtagcgaa gagagggctc
      841 gcgctgggaa tgaaggtgat gggtatcagg cggcgcccgg gccaaggcgg tcccacagga
      901 gtgaaggtcg ttggtggatt tgaggcgctg gggaccctcg cgctggaaac cgacatcctg
      961 gcgattacag tcccccgaac gcctgaaacc gccgggacgg tggacgccgg ccttctcgga
     1021 aagcttcctc gtggtgccgt cgtcatcaac gtgtcacgcg ggggcatcct gaacgaggac
     1081 gccctgctcg ccgcgttgga cagtgggcac ctgagaggcg ccgccctgga cgtcttcgcg
     1141 accgagccgc tcccacccga gcacccgctg tggcgccacc cccgggtcct gatgacgccg
     1201 cacgtttcgg ccgtcacggc tcggttctgg gaccgggaaa tggccctcat cctcgacaac
     1261 gtgcggcgct atcttggggg acaccgcctg gtcaacgtcg tcgatctgga ggccggttac
     1321 tgatggctga agcttccaaa gagaaggctc cacagcccca gccgcagggg agtgggttcg
     1381 agcggatcat caaggccgcc gtggatcgtg gcgcatccga cctgcacatc aaggccgggg
     1441 acgtcttccg ggccaggatc gacgggaaac tggtgccgct caccaagcag cggctcacgc
     1501 cggaccagac ccgggccatc gcgctgcagc tcatcccgag cgaggaagac cgcgcccggg
     1561 tggacaaggt ccgcgactac gactgctcct ggggcatgcc cgggatcggc cggttccgcg
     1621 tgaacatcct ccgccagcgc tcgtcgttca tgatcgtgat gcgggtgatc ccgttcgacg
     1681 tgccctcttt cgagtcgttg aagctccctc cggcgctcaa ccaggttgcg tcggcggagc
     1741 gggggatgat tctggtgacc ggcgtgaccg ggtcggggaa gagctcgacc atggccgcga
     1801 tcatcaatca catcaatcag aatcagcaca agcacatcct gacactcgag aacccgatcg
     1861 agtttctcca ccgggacgtg aactgctcgg tgacccagcg ggagatcggt accgataccg
     1921 acgacttccg catgggcctg cgcgcggcgc tgcggcagga cccggacgtc gtgctgatcg
     1981 gggaaatgcg cgatgcggag acgatcgata cggccatgaa ggccgccgag acggggcacc
     2041 tgctcatctc cacgctgcat acgccggacg ccgtgacgac catcagccgt atcgtggcga
     2101 tgttcccgcc tgaagagcag gatatctgcc ggatccggct cgccgataac atccaggcgg
     2161 tcgtctcgca gcggctgttg ccgcgtgccg acggcaaggg ccgggcggcc gcactcgaga
     2221 tcctgatcgc cacgggcacc gtccgcgaca tgattcggga cccgaaccgc acgcccgagc
     2281 tgcgcgacta catgcgtgag tcccgcgagc agtacggcat gcagacgttc gaccagcatc
     2341 tcatggatct cgtggccgag gggacggtga cctacgagac cgcgatcgcg acgtcctcca
     2401 atcccgccga cttcgagctg cagatgcgca cccttcggcg ccgctcgcgc acggtgcagg
     2461 cgggggacgt ggtccccgag ggcttcacgg acggcatctc cggcctttcg ccgcagtgac
     2521 cctcacgcgc gtcacgggaa ttctcgcgcc ggtcacgacg ccgttcggca tgtcgggcga
     2581 gatcgacacc gccgcgctcc agcagaacat cgcgaaggga ctcgccgacg gattgtcggg
     2641 cgtcgtcatc gccgggtcga cgggcgaggc gcccttgctc gagcgtgagg aacagcgccg
     2701 gctggtgttc gcggcgcggg agaccgttcc cgcggaccgg gtcctgctgg taggcaccgg
     2761 tgccgagtcg acccgggccg cggtcgcgct ctgcaaggat gccggagccg agcacgccga
     2821 tgcggtgctg gtacgtcctc ccgggtatta cgctccgatg ctggatgcgc gcgccgtcct
     2881 ggatcacctg cgcgccatcg ccgacgccag tccggtaccg gtactgctct acaacattcc
     2941 caagtacgct ccggtcgcga tcacgctcga gatgctcgcg aagatgcagg agcatccgaa
     3001 cgtgatcggc atcaaggact cgagcggcga ccccgcgaat ctcgccgcct atcgcaccgc
     3061 ggtccccaag tggtcggtgc tcgtgggatc ggcgtcgcac ttgaagcgag ccatcgagct
     3121 caagtgcgag ggtggcgtgc tgggtgccgc gtgcttcgcg accgcgctct gcgtcgcact
     3181 aaagaagaag gcggagctgg gggaccccga ggctgagccc ctgcaagtgc gggtggccac
     3241 cgtcgacaag gagatcgtgg gcaggctggg acccgcgggg atcaaggtgg cgatggaccg
     3301 cgtggggctc atcggcggca acccccggcc tccattgcgg ccactgactg agggcgagcg
     3361 ggaccgggtg gcggcgctgg tcgcagcgtg actcccggtt tgggcggccc cgagctgttg
     3421 tctcgaatcg ccattccccg cgccaccgga accgagggca atcgagcggt ccgggagctg
     3481 ctcaccgaag cgctgcaggc gcgtggcttc gaggtcgaaa cccaggagtt cgcggccggt
     3541 cccgatgcgc tggttgcggt acagctcgcc ggagccctgt tgggtggggg cgcattcgcg
     3601 gccctgctct tgtcccagat ccccggctgg cacggactcc tccccgtctt cgccctggcc
     3661 ctgatcgccg ggatcggcat ctacctccag cgagcctcca acaagccacc cgcccgacgt
     3721 gcgaccgggg tcaacctgat cgcgcggaag ggaactgggc tgccgacggc ctggctcgtc
     3781 gcacattacg actcgaagag ccagcccctg tccatggcgg ggcgaatcat cgcgggtggc
     3841 ttgtcggtgg ccggggccgc cggcgttctg gtattcgctg ccgccccgtc ccccatggcc
     3901 gccatcctgc tgctcccggc catgctgggt ggggcgatgc tcgcgaggtg ccgggtcgaa
     3961 aaccggtccc ccggggcgct ggacaacggc tcggcgctgg tggccatctt cgaaatcctg
     4021 gaccgcgccc cgaacgccag gatcggtgtg ctgctcccgg acgccgagga gtggggactg
     4081 ctgggcgcga gggcggcggc gcgggagcgt cctgatctct ttggtggcag gccggtgatc
     4141 aatctggatg gactggatga ccggggggcg ccgattctct tcgtccaccg cccgggagct
     4201 ctggtggaac gcctccggag tcagctgcgg gcccgggtgg cccggaggct ccccgtgttc
     4261 gtcgacggga ttgccctggc tccggtggcc ggggagtgcc tcacgatcat gaaggggaat
     4321 tgggccagcg cccggatcgt ccatacgccg cgggacgttc cgggctccct gaaactgacc
     4381 ggcgtcaaag acgtggcggc gggcgttgcc gccgtgctcg gcggccgata gatttccgca
     4441 gaggatgctc caatatgccc cgcgccgtcg gggctttttt gttgccgggg tccagagagg
     4501 gacaacagcg tgttcggtcc gaagagtcat tgcgtcgtgg tcgtcggcgc ccaatggggc
     4561 gacgagggca agggcaagat cgtggacgtg ctcgcggagc gcgccgacct ggtcgttcgc
     4621 taccagggcg gggcgaatgc gggccacacg gtcgtgaccg ggaccgaaga attcattctc
     4681 cagcaagtgc cgtcgggagt gctgcaggga gccgtgtgca tcgtgggcaa tggggtcgcg
     4741 ctcgatcccg atacgctgtt catggagctc gatgcactgg ccaagcgtgg cgtgcagtgg
     4801 gaccacaagg tgctcgtttc ggaccgcgcg catctgacgc ttccgtacca caagctgctc
     4861 gaccgggccc gtgagaagca ggagaagatc ggcacgacgg ggcggggcat cggccccacg
     4921 tacgaggaca agtacgggcg ccgcggcatt cgtgtgggcg acctccggaa tctggagcgc
     4981 gccaagaagc tcgtcgaaga gcgggtcgcc ggcgcgaacc aggtgctcgg catgctggga
     5041 gccgccgatc gggccgatcc gaaggagcac tgcgcgctcg ttgatcgcct cgcgccgcgg
     5101 cttctcccca tcaccgttga tgcgggccgc atcgtctggg acgcgctggg gcgcggagaa
     5161 tcggtcctgc tcgaaggggc ccagggagcc ttgctggacg tggatcatgg gacctatccc
     5221 tatgtgacct cctcgaacac gacggcgggt ggcgccgcgg tcggggccgg gatcgggccg
     5281 accgccatcg atgcggtgct gggtgtcgtg aaggcgtata cgacccgcgt cggaaatggt
     5341 cccctcccga ccgaagcgac ggacgcgctg tcagaacgga ttcgcgagct cggcggggag
     5401 tacggcgcgg taacgggacg gccccggcgc tgcggatggt tcgatgcggt ggtggtgcgg
     5461 tacgccgtgc gggtgaacgg actcaccggg ctggccgtaa ccaagctgga tgtgctcgac
     5521 accttcgccg aaatcccggt gtgcgtgggc tacaaactgg atggcgaagt gttcgactcc
     5581 atgccggcgg acctcgagcg gatcgagcgg gtagagccca tttacgagac cttgcccggg
     5641 tggcagtcgc cgttggggga agcacggcgc ctggccgatg tgcctccggc ggcgcgagcg
     5701 tatctggacc ggctgcagga cctggcccac gcgccaattc ggtacgtcag cgtggggacc
     5761 cggcgggatc agatcatcga agtcggctga cccttcagga ggagcgaatg gggaagagtg
     5821 acgaacagca gatggcgaag ctcgtgaaga agctctcgca gcaggcgcag aaaccgaaga
     5881 agaacctgcg ccgccaaggc gacccgactc tgcctccgga gaccacggac gcggatatgg
     5941 agcgccagga cttcttcaaa gagatgaaga agcgcgagtt ctaggtccct gaaaagcgaa
     6001 accccctggg tttccccagg gggcgctcgc ccatccgaca ccgtgcgccc tagaacccga
     6061 tgcgaacggg acgtgcgccg tacttaaaag caaaaagaat gccctgtcgc aagtcacgcc
     6121 actgcaacgc catcaaattc tccgatcgcc attttactgc cgcaatatgc ggcaaatcaa
     6181 tgcatattgc gaataaatag tcatattgct ggcgcaacgt tccacccagt agcaacttag
     6241 aaccaatgcc agccattctg gttgcgcctg ccgcgttcaa ggggacctac ggtccgcggc
     6301 aagtcgccga tgcaatcgtg gctggcgtcc gcagggccat ccccaatgcc aaggtgctcg
     6361 cctgtcccgt cgccgacggc ggggacgggc tcctcgaggc cgtgcttccg ccaggctctc
     6421 ttctcgagcg cgtcaccgtc actggtccct tgggggcgcc agtcgccgcc gagctgggtt
     6481 ggctggatgc cgaaacggcc atcttcgcgt ccagctccgc gtgcgggctc gccctcatcc
     6541 cgcccggcca gcgcgctcca ctcattgcca cgacccgtgg ggtgggcgag ctgatatggg
     6601 aggccgccga tcgcggagcg aagacggtgg tggtggggct gggaggatcg gcgacggttg
     6661 acggagggac tggagctgcc cgcggcctcg gctggagctt cgtcgatgcc accggggcgg
     6721 agcttgctga cggagggggc gcgctgggag atcttgcggt catttcgggt gggtggcgcc
     6781 tcggtgcgca ggtggtggcg cttaccgatg tcaccactcc cctggctgga ccagctggag
     6841 cggccgtcat tttcgggcct caaaagggcg cctcggatag cgaagtggaa cggctgtccg
     6901 cgggattgag ccggttggcc gagtgtctcg cccaggccgg ccacccgggc ttggacgtgc
     6961 tccccggagg cggtgccgcc gggggattag gggcagggct cgcagccttc gctcgagccg
     7021 cgctcactcc cggtgcggcc tgggtgctcg accggattgg ctttgacgct gctctggcga
     7081 ccgtcgacct ggtgatcacc ggcgaagggg aattcgacgc tacctcattc gcgggcaaag
     7141 tgaccggcga gattgtgcgc cgcgcccagg cggcgcgccg gcgcgtcgcg gtggtagcgg
     7201 ggaaggcctc aggacaggtg ggaatcaccg ttgtaagcgg aaacggcagt accctctcgc
     7261 cggccgagat cgaggggctg ggggagcggg cggcgcggga ggcgctcggg ttgcccgcgt
     7321 cctgaggcgt tcctatcttc cggcccctat gtcccctgtt gacaagacga tggaaaagct
     7381 cgtgtcgctg gccaagcggc gcggcttcg
//
LOCUS       DASUBP010000194         4274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1013780, whole genome shotgun sequence.
ACCESSION   DASUBP010000194 DASUBP010000000
VERSION     DASUBP010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013780"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(709..975)
                     /locus_tag="VFU75_07295"
     CDS             complement(709..975)
                     /locus_tag="VFU75_07295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466679.1"
                     /translation="MTNPFPGRDHARHMGELRRGDRRWDVYLEAEPTEDRASVRGRIH
                     FVAPGDQRTTGWIFLESGESEVRDRFGEFSAVELWHFLEALEPR"
     gene            1030..1734
                     /locus_tag="VFU75_07300"
     CDS             1030..1734
                     /locus_tag="VFU75_07300"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEU5466680.1"
                     /translation="MTCHACGTTLSPGVRYCHKCGAAVNSSSGGWRAGLPWGITGAAV
                     GAVITILVMRATGGVSTAAPPPEGPALTAPAGAPPDISRMSPEERARRLFDRVVELAQ
                     RGVQDSVQFFMPMALGAYAQLPSLDLDAHYDLGVLHLAGNDPNGALAEADTILASVPT
                     HLYGLMLRAQGLTEKGDAAGARKEYRAFLANEQAERARQRPEYAEHGAAIDAFHTEAT
                     NATATPAATPRSRPAR"
     gene            1760..2605
                     /locus_tag="VFU75_07305"
     CDS             1760..2605
                     /locus_tag="VFU75_07305"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MqnA/MqnD/SBP family protein"
                     /protein_id="HEU5466681.1"
                     /translation="MPTIHLAHSPDSDDAFMFWALAAGKIDTGDRKYIHELSDIESLN
                     RRALAGELEVTACSIHAYASLADRYALLAHGASIGDKYGPRVVAKTPAPADPRKALAQ
                     RVVAIPGTMTTAWLALQLYQPSVQPVVVPFDEIEDYVVAGKAEAGLLIHEGQLTFADR
                     GLHLWADMGEWWHAETGLPLPLGGNVVRRDLGESLMKAIARDLKASIQYGLKHRSPAL
                     AHAKGFGRGLDDEKVDQFVAMYVNHYTVDYGELGRKAVAALLERAQRAGLIKGPVDLT
                     FVAAE"
     gene            2635..3711
                     /locus_tag="VFU75_07310"
     CDS             2635..3711
                     /locus_tag="VFU75_07310"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEU5466682.1"
                     /translation="MAELPGTATVLVVDDEEPIRTALKKFLQQQQFDVLTAASGEEAL
                     TALRRHKLTLMLCDIRMPGTSGVDLVPQALEIEPDLAILMLTAVNDATSAALCMQRGA
                     MDYLTKPIELADLGRAIQRALKRRETLLEHRHLNQWLKEEVTTRTAELQRERHKLERI
                     SVATLEALVNALEAKDPFLRGHSARVADLAATIANEMGLTDEEVENVRVAGRLHDIGK
                     IGTRESVMNKQGPLTPEEYEHVKQHVIIGSQILAPLTHLGGVIDMVRSHHERWDGTGY
                     PDGLRGDEIPLGGRILGTAEVFDALSTARPYQDKLTPEQTIERLREMAGSVVDPKVFT
                     TLAAVVGRRKSLVFLDDGGMDHGL"
     gene            complement(3915..>4274)
                     /locus_tag="VFU75_07315"
     CDS             complement(3915..>4274)
                     /locus_tag="VFU75_07315"
                     /inference="COORDINATES: protein motif:HMM:NF023444.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family protein"
                     /protein_id="HEU5466683.1"
                     /translation="VTPLATDVLDWIAADGAAHDVTMALAVARQEAVADGRAARVRIS
                     PESLVVEVRDTGGWVPFRRFAGPATRGVSLEVSNPEVVFSPIGAGWGASNTTVTLGRG
                     KRQERITTSRVGRVKRW"
BASE COUNT          765 a         1466 c         1337 g          706 t
ORIGIN      
        1 atccgtgtcc acgaccagct tgccggcgct cgtccacagg gcttggtgct gctctccctg
       61 cgtgtctccc gggcgaggga ggagttcgca ggccgccaac agcaataacg gcgtcaagca
      121 aaggcgtcga gcaaatacgt cgagcagagg cgtaaatctg gtacgggctc ctaggggggt
      181 gcgcgccatg ttgtcggatg agcctacgcg ccgatcccag cagccttcga gtccaccagt
      241 tggcctccga cctagccgtg gatgtggata ccgtggcccg gcgcttcagg ggccctgggg
      301 ggttcgccag aggagatcag ttggcccggg cagttctctc aatcggttcc aacatagccg
      361 aggcgtgtgg tcgaggcaca gtgcgggaat ttcggcagtt tctatcctac gcacgggcct
      421 ctgcccaaga ggcgcttaca caagtccgca tctctcgacg catcgacaag cacttggatg
      481 ggaagcttct ctctctccaa ggtcgattga ttctggcaat caagatgatc gacaggctca
      541 gcgtgagccc tcccccaccc cgttaataac gtcagggcgt gaggtttttg ttcgacgcct
      601 ttgctcgacg cctttgctgg acgtctttgc tggacgtctt tgcttgacgt ctttgctcga
      661 cggttttctt ggggtgttgg gttctgcgac tgcagccggc gctcgtcctt accgcggttc
      721 cagtgcctcg aggaagtgcc aaagctcgac ggccgaaaat tcaccgaagc ggtccctgac
      781 ctcgctctcg ccggactcga ggaagatcca gcccgtcgtc cgttgatcgc cgggggccac
      841 gaagtggatc cgtccccgca cggacgcacg gtcctccgtc ggctcggcct caaggtagac
      901 atcccaacgg cggtcgcccc ggcgcagctc gcccatgtgc cgcgcgtggt cccggcccgg
      961 gaacgggttg gtcatagtgt tacagactac gctccccctg gaaatcctgc cagttagatt
     1021 cttggcgcaa tgacctgtca tgcctgcggt accacgctct cccccggcgt ccgctattgc
     1081 cacaagtgcg gagccgcggt gaacagctcg tccggtggct ggcgcgccgg tctcccctgg
     1141 ggcatcaccg gagccgccgt cggcgccgtg atcacgatcc tcgtgatgcg cgccacgggt
     1201 ggggtatcca cggccgcacc gccacccgag gggccggccc tgaccgctcc cgccggcgcg
     1261 cctcccgaca tcagccgcat gtcacccgag gaacgtgcgc ggcggttgtt cgaccgcgtg
     1321 gtcgagctgg ctcagcgcgg cgtgcaggac tccgtacagt tcttcatgcc catggccctg
     1381 ggggcctatg cgcagttgcc gtcgctcgat cttgacgcac actacgacct gggcgtactg
     1441 cacctcgccg gaaacgatcc gaacggcgcg ctcgcggaag ccgacaccat tctcgcgtcg
     1501 gtccccacgc acctgtatgg actgatgctc cgcgcgcagg gtctcacgga aaagggagac
     1561 gccgcggggg cgcgaaagga gtaccgggcg ttcctcgcga acgagcaggc ggagcgcgcg
     1621 cgtcagcgac ccgagtacgc ggagcatggc gccgccatcg acgccttcca caccgaagcc
     1681 acgaacgcca cagccacccc ggctgccacg ccgcgctctc gtccggcgcg gtagcggtct
     1741 ccttctaatc caggcgccaa tgccgacaat tcacctggcc cacagtcccg actccgacga
     1801 cgctttcatg ttctgggccc tggcagccgg gaagatcgac accggtgacc ggaaatacat
     1861 ccacgaactc tccgacatcg agtcgctcaa tcgccgggcc ctggcgggcg agctcgaggt
     1921 caccgcctgc tcgattcacg catacgcctc cctcgcggac cgctacgccc tgctcgcgca
     1981 cggcgccagc atcggcgaca agtacggccc gcgcgtcgtc gccaagacgc ccgcccccgc
     2041 cgacccccgc aaggcgttgg cacagcgggt ggtcgccata cccggtacga tgaccacggc
     2101 ctggctcgcg ctgcagctct atcagcccag cgtccagccg gtggtggtgc cgttcgacga
     2161 aatcgaggac tacgtggtgg cgggaaaggc ggaagccggg ctcctcattc acgagggcca
     2221 gctcaccttc gccgatcgcg gtctccacct gtgggccgac atgggcgaat ggtggcacgc
     2281 ggagaccgga ctgccattgc ccctgggcgg gaacgtggtg cggcgcgacc tgggcgagag
     2341 cctgatgaaa gccatcgccc gggacctgaa ggccagcatc cagtacggac tgaagcaccg
     2401 ctcaccggcc ctggctcatg cgaaggggtt cggcagggga ttggatgacg aaaaggtcga
     2461 ccaattcgtc gcgatgtacg tgaatcacta caccgtcgac tatggtgaat tgggccggaa
     2521 agcggtcgcc gcactattgg aacgggccca gcgggccgga ctgatcaagg gccccgtcga
     2581 cctaacgttc gtggcagcgg agtaataagt ttggtcagcc gctagggagg acacgtggct
     2641 gaactgcccg gtactgcaac ggtgctggtg gtcgacgacg aagagcccat ccgcaccgct
     2701 ctgaagaagt tcctgcagca gcagcaattc gacgtcctga cggccgcctc cggcgaggag
     2761 gccctgacgg cgctgcggcg gcacaagctg accctcatgc tgtgcgacat ccgcatgccc
     2821 ggcacgtcgg gcgtggactt ggtcccccag gccctcgaga tcgagccgga tctcgccatc
     2881 ctcatgctca ccgccgtcaa cgacgccacc tcggccgcgc tctgtatgca gcgcggcgcg
     2941 atggactatc tcaccaagcc catcgagctc gcggacctgg gccgcgccat ccagcgcgcc
     3001 ctcaagcgcc gcgagacact gctcgagcac cgccacctga accagtggct gaaggaagaa
     3061 gtcaccaccc gcaccgccga gctgcagcgg gaacggcata agctggaacg gatctcggtg
     3121 gcgaccctcg aggccctggt caacgcgctc gaagccaaag acccgttcct tcgtggacac
     3181 tcggcccggg tcgcggactt ggccgccacc attgcgaacg agatgggact caccgatgag
     3241 gaagtcgaga acgtgcgcgt ggcgggacgg ctgcacgaca tcggaaagat cgggacccgc
     3301 gaaagcgtga tgaacaaaca gggccccctc acgcccgagg agtacgagca cgtgaagcag
     3361 cacgtgatca tcggctccca gatcctggca cctctgactc acttgggggg cgtgatcgac
     3421 atggttcgca gccaccacga acggtgggac ggcacggggt atcccgacgg gctgcggggc
     3481 gatgaaatcc ccctcggcgg ccgcattctg ggtaccgccg aagtgttcga cgccctctcc
     3541 acggcccgcc cctaccagga caagctcacc cccgagcaga ccatcgaacg cctgcgcgag
     3601 atggccggca gcgtggtcga cccgaaggta ttcacgacgc tcgcggcagt ggtggggaga
     3661 cggaagagcc tggtattcct ggacgacggc ggcatggacc acgggctata aaaaaccaaa
     3721 cccggggccg attgctcccc gggttggcga tcgggcgaag tggccgtcac acttcggccg
     3781 cgatcaagtc ttctatgttc tgctgctccg tagtacggga gcctttgcgc agggtttcgc
     3841 gagccctcac tgcatgagat gctcgacaac catatacggt tgcaccccgc gaactgtttc
     3901 actcaacccc tgcctcacca ccgcttcacc cgtcccaccc tcgacgtcgt gatccgttcc
     3961 tgacgcttgc cgcggcccaa ggtcacggtg gtgttggaag caccccaccc agcgccgatc
     4021 ggcgaaaaaa cgacctctgg attggacact tccaggctca caccgcgggt cgccggcccg
     4081 gcgaatcgcc ggaatgggac ccacccaccg gtgtcccgca cctccaccac cagcgactcc
     4141 ggactgatcc ggactcgagc agctcgccca tccgccaccg cctcctggcg tgcgacagcc
     4201 agggccatgg tgacgtcgtg ggccgcccca tcggcggcga tccagtccag cacatcggtc
     4261 gccagtgggg ttac
//
LOCUS       DASUBP010000195         2123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_809441, whole genome shotgun sequence.
ACCESSION   DASUBP010000195 DASUBP010000000
VERSION     DASUBP010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2123
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_809441"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..262
                     /locus_tag="VFU75_07320"
     CDS             <1..262
                     /locus_tag="VFU75_07320"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5466684.1"
                     /translation="DDIETGYAFYPPWWGKGLATEIARKCIDLGFEALKLQSVVGLTT
                     PANTASQHVLRKVGMVLDREYDHAGIPHVLFRVTRQTWASTR"
     gene            273..1346
                     /locus_tag="VFU75_07325"
     CDS             273..1346
                     /locus_tag="VFU75_07325"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HEU5466685.1"
                     /translation="MLSRLRSIFNDDATKVPGRVVTLYVLVAAANAGTWAWALLAFRH
                     YPLLLGTALLAYGLGLRHAIDADHIAAIDNVTRKLMQEGKRPVGVGFFFSLGHSTIVV
                     LASVAIAATAIALHTRLAAWHSVGGVIGTIASATFLLLIGAINLVVLVSVYRTFHRAR
                     KHGQYLEEELTALLAGGGLLARLFRPMFAMIRRSWHMYPLGLLFGLGFDTATEIALLG
                     ISAAEAARGMPIWTILVFPALFTAGMSLVDTTDSILMLGAYGWAFTTPIRKLYYNLTI
                     TFVSVVVALLVGGIEALGLVGAKFALTGRFWRVIESLNSNFGSIGYLIIGVFVVSWIL
                     SVGIYRLKGYDKLEPVAVRRDPP"
     gene            1343..1891
                     /locus_tag="VFU75_07330"
     CDS             1343..1891
                     /locus_tag="VFU75_07330"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEU5466686.1"
                     /translation="MNTIRAMMLLWLIWLVSWLAAAYWSDRAVKSPGLRRQLTHNLLT
                     ISGCLLLFAFDSTDTVMRLPYAGRWATLGLAAVGFGFSWWARITLGRFWARNVSRKED
                     HRVIDTGPYALVRHPIYTGIILATIATALLKATLIAWAGVLVMTLGWYVKARLEERFL
                     HAELGEAYDAYKARVPMLVPFI"
BASE COUNT          313 a          728 c          667 g          415 t
ORIGIN      
        1 ggacgacatc gaaaccgggt acgcgttcta tcccccctgg tggggcaagg ggctcgcgac
       61 cgaaatcgcc cggaagtgca ttgatctggg cttcgaggca ctcaagctcc aatcggtggt
      121 cggactcacc acacccgcca acacggcctc acagcacgtg ctgcggaagg tcggcatggt
      181 cctggaccgt gagtatgacc atgccgggat cccgcacgtc ctgttccggg tgacccgaca
      241 gacctgggcg tcgacacgat agattccccg tcgtgctgag ccgactccgc tccatcttca
      301 acgacgatgc cacgaaggtg ccgggccgcg tggtcacact gtacgtgctg gtggccgccg
      361 cgaacgcggg cacctgggcc tgggccctcc tcgccttccg tcactatccg ctgctgctcg
      421 gcaccgccct cctggcctac ggtctcggcc tgaggcacgc gatcgacgcc gatcacatcg
      481 cggcgatcga caacgtcacc cgaaagctca tgcaggaggg gaagcgcccc gtgggggtgg
      541 ggttcttctt ttcgctcggc cattccacga tcgtcgtgct ggcgtcggtg gcgatcgccg
      601 ccaccgccat cgcattgcac acccgcctcg cggcctggca ttccgtcggc ggagtcatcg
      661 gcaccatcgc gtcggccacc tttctcctgc tcatcggcgc gatcaacctc gtggtcctgg
      721 tctctgttta ccgcaccttc catcgggcac ggaagcacgg gcagtacctg gaagaggagc
      781 tcaccgcgct gctggccggc ggcggcctcc tcgcccggct cttccggccg atgttcgcga
      841 tgattcgccg gtcctggcac atgtacccgc tgggactcct cttcggactc ggcttcgaca
      901 ccgcgacgga gatcgccctg ctcggcatct ccgcggccga ggccgcgcgg gggatgccga
      961 tctggacgat cctcgtgttc cccgccctgt tcaccgccgg catgtccctc gtggacacga
     1021 ccgactcgat cctgatgctt ggtgcctatg gctgggcctt cacgaccccc atccggaagc
     1081 tctactacaa cctgaccatc acgttcgtct cggtcgtcgt ggctctgctc gtcgggggaa
     1141 tcgaagcgct ggggttggtc ggcgcgaagt tcgccctgac ggggcgattc tggcgggtca
     1201 tcgaatctct caacagcaac ttcgggtcga tcgggtacct catcatcggc gtcttcgtcg
     1261 tcagctggat cctgtcggtg gggatctacc ggctgaaggg ctacgacaaa ctcgaaccgg
     1321 tcgcggtccg cagggatccg ccatgaacac catcagggcg atgatgctgc tctggctcat
     1381 ctggctggtc agctggttgg cggcggcgta ctggagtgac cgagccgtca agagtccggg
     1441 gctgagacgg cagctgaccc ataacctgct gaccatctcc ggctgcctgc tcctcttcgc
     1501 cttcgactcg accgataccg tgatgcgcct cccgtacgca ggccggtggg ccacgctcgg
     1561 gctggcggcg gtcgggttcg ggttttcctg gtgggcacgg atcacgctcg gccgtttctg
     1621 ggcgcggaat gtctcccgga aggaagatca tcgcgtgatc gacacgggcc cctacgcgct
     1681 cgtgcgccat cccatctata cgggaatcat tctcgccacc atcgcgacgg cattgctcaa
     1741 ggcgacgctg atcgcgtggg cgggggtcct ggtgatgacg ctcgggtggt acgtcaaagc
     1801 ccggctcgag gagcgattcc tgcatgcgga gctcggcgag gcgtacgacg cctacaaagc
     1861 ccgggtcccg atgctggtgc cgttcatctg agactcccgc gcgccgggcc gcctgcgcgc
     1921 cgcgctgagt ggtggtagcg cgcctctcgg atctcctcga ctttggtggc catgcgtctc
     1981 acccccgtca aagccattct gctcggcgga ctgaccgtgg ctgtcctcga catcgccgac
     2041 gccttcatct tctacgcgtt ctatggcgcc acgcccgtca ggattctcca atcgatcgcg
     2101 gtcggcgtgc tgggacgcga cgc
//
LOCUS       DASUBP010000196        12981 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1013900, whole genome shotgun sequence.
ACCESSION   DASUBP010000196 DASUBP010000000
VERSION     DASUBP010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 12981)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12981)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12981
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1013900"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..899)
                     /locus_tag="VFU75_07335"
     CDS             complement(<1..899)
                     /locus_tag="VFU75_07335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466687.1"
                     /translation="MAKTPSKGTVAIQRLLEERKQLESWIARIDAAADATPAAVRGRV
                     RSDYEARLVTVIKELKNHVEAAREVIAQRRAVLGDLQAKEAAAAEALSEAELRHAVGE
                     YDESQWTQVHKEALAELVSIREELQVVQVEVEKLQEIDALATEAAPPKPAPKPAPPPP
                     PAPPVAEASLTVVSDRGKGNAGTPSRTARPEEKKHEPVDELAFLKSVTEDARGGGISQ
                     RRASGAQYQPVEPGVTPAAPPPPAPPSPPPMSTRAPRITQEADPVIAESGEEPVKTLK
                     CKDCGTMNLPTEWYCENCGAELAA"
     gene            complement(950..2236)
                     /locus_tag="VFU75_07340"
     CDS             complement(950..2236)
                     /locus_tag="VFU75_07340"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEU5466688.1"
                     /translation="MNRPLLLKGGRVIDPSRDVDQVADLLVQDGKVAGIGQGLGAPEG
                     AETRDVKGMIVAPGLVDIHVHFREPGNEDEETVATGARAAVTGGYTAVCAMPNTDPVT
                     DNQAAVGFIVRQSVRAGLARVYPIGAVSVGQKGEKLAEFGEMVGAGAVAVSDDGKPVA
                     SSHLMRTALEYARTFEIPVADHCEEPTLFAGGVMHEGLVSSRLGLKGIPAAAEEIMVA
                     RDLLLAELTGGHVHLCHMSTRGSVELIRRAKERRVRVTAEVTPHHLTLTDHACEGYDT
                     HAKMNPPLREAADVAALRQGLVDGVIDCIATDHAPHAYDEKDAAFDDAPFGIIGLETA
                     FGVAYTELVEGGVLTLPELINRMSTAPARAFHLTGGGLRIGAVADICVLDVSKPWTVD
                     PAQFHSKSRNSPFGGRRVTGRVRMTLVAGRIVHDTP"
     gene            complement(2233..3195)
                     /locus_tag="VFU75_07345"
     CDS             complement(2233..3195)
                     /locus_tag="VFU75_07345"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEU5466689.1"
                     /translation="MMTLGKDLVGLEHLSPEQIRLILDTAEPFKEVSERPIKKVPALR
                     GKTIVNLFFEASTRTRISFEFAEKRLSADTVNVAASGSSVSKGETLVDTARNLEAMRI
                     DMVVIRHGSSGAAQFLGSRIRSNVINAGDGKHEHPTQALLDLLTIRDRHGRIEGLKVC
                     LVGDILHSRVARSNIWGLTKLGAEVGVCGPRTLMPRGIEELGVRMFDRIEAAIEWADV
                     LNVLRLQLERMEKGFIPSLREYYRVFGVTEERLAQSKRDITIMHPGPMNRGVEIDSRV
                     ADGPHSVILDQVTNGVAIRMAVLYLLAGGKPELADAAKRSGGAE"
     gene            complement(3192..3746)
                     /gene="pyrR"
                     /locus_tag="VFU75_07350"
     CDS             complement(3192..3746)
                     /gene="pyrR"
                     /locus_tag="VFU75_07350"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEU5466690.1"
                     /translation="MTPAQDRSILLDARAFARALQRMAVEILELAPAADQLVLIGIQR
                     RGVELAERLAQMIEKDEGVSIPRGALDITLYRDDLETVGPKPVIGETRIPGDLSGRHV
                     VIVDDVLYTGRTVRAALDELADFGRPRRISLCVLVDRGGRELPIAADVVGKSVKTGPK
                     DRVDVSVQELDGKDEVTVLRGTGS"
     gene            complement(3765..4691)
                     /locus_tag="VFU75_07355"
     CDS             complement(3765..4691)
                     /locus_tag="VFU75_07355"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HEU5466691.1"
                     /translation="MFVAAELRELLDPSRLEGLDVTWVRSDEPTPEGEFLGAVPLLTR
                     WMGGTEFKRLPKLRIVANCAVGHNNVDLVAAEMRRIVVTNTPDVLTAATADLTWALIL
                     ACARRLLAGVDMVKTGNWTGWHPQMLLGLELEGATLGIVGAGRIGQAVGKRALGFGMR
                     ITYASRTPKPEFERATGATRLELRPLLRESDVVTLHVPKTPETQGMINADALAAMKPG
                     AILINTSRGDLVREDAVAAALEQGRLGAAGLDVFAEEPDIHPRLRAAPRTVLLPHIGS
                     ATLPTRRKMAETAVANVQAVLAGAPPLTPVVP"
     gene            complement(4703..6229)
                     /locus_tag="VFU75_07360"
     CDS             complement(4703..6229)
                     /locus_tag="VFU75_07360"
                     /inference="COORDINATES: protein motif:HMM:TIGR02262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016405 - CoA-ligase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate-CoA ligase family protein"
                     /protein_id="HEU5466692.1"
                     /translation="MVFEPPERFNIADYFLGARIAEGLGARPALLTDQGTYTYAEVDR
                     LASRFAHLLREAQVSPEQRVIIALPDGPAFVGALFGILKIGAVVVMVNPQLTPEQIAY
                     FYDYTRATAVLVAGPSAPAFVAAAAGAPTLKRVLKVDAPDVTLRLEALPDSIETFPTH
                     RDDPAIWLFSGGTTGQPKAVVQTHGSYANTTECYAKNTVGYRADDITVSVPKLFFGYA
                     MGANLFFPFSVGARVILFEEPSTPEALFTRIAKYRPTVLINVPTMVNRMVSHPDAPRQ
                     DLSSLRFATSAGEALPVELYHRWKETFGVELLDGLGTAEMWHIFLTNRVGAVKPGTIG
                     QVVAGFEVKAADDSGVEVPAGEVGLLWVRGHSQGLGYWQLQEKTRRTFRGDWVVCGDL
                     VSRDADGYFTHHGRADEMLKVSGKWLSPQEVESCLLQHPAVREAAVVGITGDGGLVKP
                     HAYVVTLDRRADLAEELKAFVRERLEPFKAPREISFVDALPRTHLGKVDRGRLRRGAA
                     "
     gene            6247..6987
                     /locus_tag="VFU75_07365"
     CDS             6247..6987
                     /locus_tag="VFU75_07365"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEU5466693.1"
                     /translation="MPVRELGELSWTDIQALDRSRVVAILPLGAVEAHGPHLPLGTDV
                     IIAEAMARAGAELLLPQGIQPLLLPALPYAPAPYAADFPGTISIDPHAVTSTIQAIAS
                     SLSRHGIRFLALANAHFDPVQVRAVRDAVHGTEAMVIFPDLTRRALAQRLTAEFQSGA
                     CHAGRYETSIVMAARPELVHETLRTALPANNRSLVEAHAAGQTTFRAAGGADAYFGAP
                     AEASATEGRESIAKLGEILADAVREVMR"
     gene            6984..7757
                     /locus_tag="VFU75_07370"
     CDS             6984..7757
                     /locus_tag="VFU75_07370"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5466694.1"
                     /translation="MSAAVLAGKRAVITGGGRGIGAAVARALADAGVGVLVAARSRAE
                     VEAVANALRAQGHRASAATADVTRPESVNALARTATEVLGDIDILINNAGSSSSAPLK
                     RTTLEEWNRLLAVNATSTFLCTQAFLPGMLDRKWGRVVNLASVAGLFGAKYIAAYSAA
                     KHAVVGFTKSMAAEVAGSGVTVNAVCPGYVDTPMTDQSLERMMQTTGRSRAEALEAVL
                     AHSGQSRLITPDEVARAVMAVCNGSETGATVLLDGKGPA"
     gene            7754..8137
                     /locus_tag="VFU75_07375"
     CDS             7754..8137
                     /locus_tag="VFU75_07375"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU5466695.1"
                     /translation="MSFDVINPDPPGRPKGWNNGMLAPAGGRVLFVAGQTAPTAGGLV
                     AQWTMALSQVLSIVRASGGGPTDIGRMTVYVTSKADYLANLQALGEVHQKAMGKHYPA
                     MALVEVSALVDPAAVVEIEATAVIG"
     gene            8140..8940
                     /locus_tag="VFU75_07380"
     CDS             8140..8940
                     /locus_tag="VFU75_07380"
                     /inference="COORDINATES: protein motif:HMM:NF006107.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HEU5466696.1"
                     /translation="MAILPKSFLYEVDSGTGVATITLNRPDRLNALTFQVYEELRDVF
                     RALDSEAGVRAIVITGSGRAFCSGGDVEDIIGQLFSRDAKGLLDFTWLTCDLILAMRL
                     CRRPIVAALNGTVAGAGAVIAAACDIRIAAESARIAFLFTRVGLSGADMGAAWLLPRL
                     VGLGRATELLMTGDFIDASRALEIGLYNRVVPVDRVVAEAREFAARLALGPSKALAVT
                     KEALNREGAMDLEQALELEAKLQAELMQHPNFRESYEAFKAKRQPKFQ"
     gene            8940..10097
                     /locus_tag="VFU75_07385"
     CDS             8940..10097
                     /locus_tag="VFU75_07385"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5466697.1"
                     /translation="MLPDPRPVRAFLDPKHIDFAARVAEFAAREIATLPAPADDPAAR
                     VQAKEILTRLGSGGWLAPIGDQDWRSCCLVREALGAASPLADAVFALQALGTLPIMMG
                     STDDLAARWLTAALQGRAMGGFAMTEPEAGSDVAAMATTARRDGAFYVLDGVKTLISN
                     AGIADYYAVYAVTDRSKGTKGITCFVVPADTPGCRFVRPLILSSPHPLGEMAFESCRI
                     PAGQRLGAEGEGYRLGLATLDRLRPTVGAAACGMASRALSEALDHATARKQFGRPLAD
                     FQLVQEKLSRMATDLTAARLLVYRAAWEKDHGAERITLEAGMAKAFATEAAQRIIDDA
                     VQILGGRGVLADHPVDRLYRSVRALRIYEGTTEIQHLVISGQLLKQHRGES"
     gene            10094..12346
                     /locus_tag="VFU75_07390"
     CDS             10094..12346
                     /locus_tag="VFU75_07390"
                     /inference="COORDINATES: protein motif:HMM:NF006101.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003959 - NADPH dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HEU5466698.1"
                     /translation="MKIVCVGGGPAGLYFSILMKKADPSHDILVVERNRFEDTFGFGV
                     VFSEATQENLQAADPETVDAMAKASAHWDDIDIHYKGGVIRSTGHGFSGLSRRAMLEI
                     LAQRARGLGVQVEIGRDVTDLKDYLKADLLLAADGANSMVRERYAKHFQPSVDLRPNR
                     FVWLGTTRPFPAFTFYFKHDRHGLWRVHTYQYEKGHSTFIVEAREETWRAACLDRASE
                     EQTIAFCEELFAEELAGHRLQKNRSIWRQFGTIRNAHWHHENVVLIGDSAHTAHFSVG
                     SGTKLAMEDAIVLARSLAGHGNRDTALNAYEAERRPLVESLQRAAQVSLAWFEDTERY
                     MDMDPVQFGFNLLTRSLRITHDNLKVRDPKFVAGVDAWFAKGAARQSGVAIPAEPVPP
                     PLFTPFRLRELVLENRSVVSPMCQYSAEDGTPNDWHLVHLGTRAIGGAALVMTEMTDV
                     SREGRISTGCTGMYKPEHVVAWRRIVDFVHGASRAKIGMQLSHAGRKGSTRKLWEGDS
                     LPLESGGWPIMAASAIPYYPNSPVPKEMDRRDMDRVRDEFVQSARMTEEAGFDLLELH
                     FAHGYLLASFISPLTNVRKDEYGGTLEARMRYPLEVFDAVRAVWPRQKPMSVRISAVD
                     WAPGGMESEDAVQVARLLKAHGCDILDVSAGQTVPFQSPVYGRLYQTPFADRIRHEAE
                     IPVMTVGNISSYTDVNTILAAGRADLCVLARAHLWDPYWTRHAARELGWEIEWPDQYE
                     SLDRYKPRFT"
     gene            complement(12354..12716)
                     /locus_tag="VFU75_07395"
     CDS             complement(12354..12716)
                     /locus_tag="VFU75_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466699.1"
                     /translation="MIQTPPPGIPDIPFDWNLFINNTLAPIVVTVIVGVGFLIALRVI
                     MKSPVGEAWAERIRRKTNIKFGEPSGLSGEQTAIQLDGFQDQLGRIESHLAEMNERLD
                     FTERVLTKKDPNAIGPGR"
     gene            complement(12751..>12981)
                     /locus_tag="VFU75_07400"
     CDS             complement(12751..>12981)
                     /locus_tag="VFU75_07400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466700.1"
                     /translation="VGFFAAYVAGRALPAGSPYLSVFRFVGVTAFLAYTLALWQMTIW
                     YNRALMTSIKVTVDGLIYGLLTAGMFGWLWPK"
BASE COUNT         2094 a         4499 c         4254 g         2134 t
ORIGIN      
        1 gccgccagct ccgccccgca attctcgcaa taccactccg taggcaggtt catcgtcccg
       61 caatccttgc acttcagcgt cttcaccggc tcctcacccg actcggcgat gaccggatcc
      121 gcctcctgcg tgatccgcgg cgcccgcgta gacatgggcg gcggcgacgg aggcgcggga
      181 ggaggaggcg ccgcaggagt caccccgggc tcaacgggct gatactgcgc gccgctcgcc
      241 cgtcgctggg atatgccacc tccacgggcg tcttcggtga cggacttgag gaaggccagc
      301 tcgtccaccg gctcgtgctt cttctcctcc ggcctggcgg tacgggacgg cgtgcccgca
      361 ttccccttcc cgcgatcgga cacgacggtg agggacgctt cagccaccgg cggcgccggg
      421 ggtggcggag gagcgggctt cggcgcgggc ttcgggggcg ccgcctcggt agccaaggcg
      481 tcgatctcct ggagcttctc cacttccacc tgcaccacct ggagctcttc gcggatcgac
      541 accaactccg ccaacgcttc cttgtgtacc tgcgtccact ggctctcgtc gtattcccca
      601 accgcgtggc gcagctccgc ctcggacagc gcctcggcgg ccgcggcttc cttcgcttga
      661 aggtcgccga gcacggcccg gcgctgcgcg atgacctccc gcgcggcctc cacgtgattc
      721 ttgagttcct tgatgacggt gacgaggcgg gcttcgtagt cggagcggac acgcccccgc
      781 acggcggcgg gcgtagcatc cgccgccgca tcgatgcggg cgatccagga ttcgagttgt
      841 ttccgttcct ccagcaggcg ctgaatcgcg accgtgcctt tggaaggcgt cttagccatc
      901 agggcgctcc aactgcgtgt aatgagcggt cagggcacaa catacaaatc tatggcgtat
      961 cgtgcacgat acgtccggcc accagggtca tccggaccct gccggtcacg cgacggcccc
     1021 caaaggggga attccggctc ttggagtgga attgggccgg gtccaccgtc cagggcttgg
     1081 acacgtccag tacgcaaata tcggccacgg caccgatccg gagcccccct ccggtcaggt
     1141 ggaacgcccg cgccggggcc gtgctcatcc ggttgatcag ttccgggagg gtcaggaccc
     1201 cgccctccac cagctccgtg tacgccacgc cgaaggccgt ctcgagtccg atgatgccga
     1261 agggggcgtc gtcgaaggcg gcgtccttct cgtcgtacgc atggggagcg tggtcggtcg
     1321 cgatgcaatc gatcacgccg tccaccaggc cttgccggag ggccgcgacg tcggcggcct
     1381 cccgcagggg cggattcatc ttggcgtgcg tgtcgtagcc ttcgcaggca tgatcggtca
     1441 aggtgaggtg atgcggcgtc acctccgcgg tgacacgcac ccgccgctcc ttcgcccggc
     1501 gaatgagctc caccgacccc cgcgtcgaca tgtgacagag gtgcacgtgt ccgccggtca
     1561 gctcggcgag gagcaggtca cgcgccacca tgatctcttc ggcggccgcc ggaatgccct
     1621 tgagtccgag tcgcgaggaa acgagtcctt cgtgcatgac gccgccggcg aagagcgtcg
     1681 gctcctcgca gtgatcggcg acggggatct cgaaggttcg cgcgtactcg agcgcggtcc
     1741 gcatcaggtg actcgaagcg accggcttcc cgtcgtcgga caccgcgacc gctccggccc
     1801 ccaccatctc cccgaactcg gcgagtttct cgcccttctg ccccaccgac accgcgccga
     1861 tcggatagac gcgcgcgagc cccgcccgca ccgactgccg cacgatgaac ccgaccgccg
     1921 cctgattgtc ggtcaccgga tcggtgttcg gcatcgcgca caccgccgtg tacccgcccg
     1981 tcaccgcggc gcgggcgccg gtggcgaccg tctcctcgtc ttcgttgccc ggctcgcgga
     2041 agtgcacgtg gatgtccacg agccccggag cgacgatcat gcctttcacg tcgcgcgttt
     2101 cggcgccctc cggcgcgccc agcccctgcc cgattccggc gaccttgccg tcctgcacca
     2161 gcaggtccgc gacctgatcg acgtcgcggc tcggatcgat gacgcgtcca cccttgagca
     2221 ggagcggccg gttcactcgg cgcctccgga gcgcttggcc gcgtcggcca gctcgggctt
     2281 gccgcccgcg agcagataga ggaccgccat ccggatcgcc acgccgttgg tcacctgatc
     2341 gaggatcaca ctgtgcgggc cgtccgccac ccggctgtcg atctcgacgc cgcggttcat
     2401 cgggcccggg tgcatgatgg tgatgtcccg cttcgactgc gcgagacgct cttcggtcac
     2461 gccgaagacg cgatagtact cgcgcaggga cgggatgaat cccttctcca tccgttcgag
     2521 ctgcagccgg agcacgttga gcacgtcggc ccattcgatc gccgcttcga tccggtcgaa
     2581 catcctgacc ccgagctctt cgatgccgcg cggcatcagc gtgcgcggcc cgcagacgcc
     2641 gacctcggcc ccgagcttgg tgagacccca gatgttggag cgggccacgc ggctgtgaag
     2701 gatgtccccg accagacaga ctttcaggcc ttcgatccgt ccatgacggt cccggatggt
     2761 gagcaggtcg aggagcgctt gcgtcgggtg ctcgtgcttg ccgtcgccgg cattgatgac
     2821 gttcgagcgg atccggctgc ccaggaactg cgccgcgccc gaggatccgt gccggatcac
     2881 caccatgtcg atgcgcatgg cctcgagatt ccgcgcggtg tcgaccaggg tctccccctt
     2941 cgagacggac gaacccgacg cggcgacgtt caccgtgtcc gccgacagac gcttctcggc
     3001 gaactcgaac gagatgcggg tccgcgtcga cgcctcgaag aagagattga cgatggtttt
     3061 gccgcggagc gccggcacct tcttgatcgg ccgttccgac acttccttga acggctcggc
     3121 ggtgtcgaga atgaggcgaa tttgctccgg cgaaaggtgc tcgaggccga cgagatcctt
     3181 gcccagagtc atcatgaacc cgtcccgcgc aacaccgtca cctcgtcctt gccgtccagc
     3241 tcctgcacgg agacatccac ccggtctttc ggacccgtct tcacgctctt ccccacgacg
     3301 tcggcggcga tcggaagctc gcggccaccc cggtccacca gtacgcagag cgagatgcgg
     3361 cgcggacgcc cgaaatccgc cagctcatcg agcgcggccc ggacggtccg gccggtatag
     3421 agcacgtcat ccacgatcac cacgtgccgg cccgagagat cacccggaat ccgggtctcc
     3481 ccgatcaccg gcttgggccc cacggtctcg agatcgtcgc ggtacagggt gatatcgagc
     3541 gcgccgcgcg ggatggacac gccttcatcc ttctcgatca tctgagccag ccgctcggcc
     3601 agctccacac cgcggcgctg aatgccgatg aggaccagct gatcggcggc gggagcgagc
     3661 tccagaatct cgaccgccat acgctgaagc gcccgcgcaa aggcacgggc gtcgaggaga
     3721 atgctgcgat cttgtgcggg ggtcattccg agcgtgaaag gtaactaggg caccaccggg
     3781 gtcaacggcg gagcccccgc gagcacggcc tggacgttgg ccaccgccgt ctcggccatc
     3841 ttgcgccggg tgggcagggt tgccgagccg atgtgcggta ggagcaccgt ccggggcgcg
     3901 gcccggagac gaggatggat atcgggctcc tcggcgaaca catcgagacc ggccgcgccc
     3961 agccggccct gctccagcgc cgcggcgacc gcgtcttccc gtaccaggtc gccacgcgac
     4021 gtgttgatca ggatcgcgcc cggcttcatc gcggccaatg cgtcggcgtt gatcattccc
     4081 tgcgtttccg gtgtcttcgg aacgtgcagc gtcaccacgt cgctctcacg cagaaggggt
     4141 ctcagctcca gacgggtcgc gccggtcgcc cgctcgaact ccggcttcgg tgtccgggac
     4201 gcgtacgtga tccgcatgcc gaaccccaac gcgcgcttcc ccaccgcctg tccgatccgt
     4261 cccgcgccga cgatccccag cgtggcgcct tcgagctcga ggcccagcag catctggggg
     4321 tgccatccag tccaattccc ggtcttcacc atgtccacgc ccgcgagcag ccggcgcgca
     4381 caggcgagaa tgagcgccca ggtgaggtcg gcggtcgccg cggtcagcac atccggcgtg
     4441 ttggtgacga cgatccggcg catctcggcg gcgaccagat cgacgttgtt gtggccgacc
     4501 gcgcaattcg cgacgatgcg gagcttcggg agtctcttga attccgtgcc ccccatccag
     4561 cgggtgagca gcggcacggc cccgaggaac tccccctccg gcgtcggctc gtcggagcgc
     4621 acccaggtca cgtcgagtcc ctcgaggcgt gacgggtcca gcaattcgcg cagctcggcg
     4681 gcgacgaaca ccgggaaccc gctcaggcgg ccccccgccg caaccggccg cgatcgacct
     4741 tgcccaggtg cgtccgcgga agcgcgtcga cgaatgagat ctcgcgcggg gccttgaagg
     4801 gctcgaggcg ttcgcgcacg aacgccttca gctcctcggc gagatcggcg cggcggtcga
     4861 gcgtgacgac ataggcgtgg ggtttgacga ggccgccgtc accggtgatg ccgacgaccg
     4921 ccgcctcacg gacggccgga tgctggagga gacagctttc gacctcctgc ggcgagagcc
     4981 acttgcccga gaccttgagc atttcatcgg cccgcccgtg gtgggtgaag tacccgtcgg
     5041 cgtcccgcga gacgagatcc ccacacacca cccagtcgcc gcggaacgtg cgccgggtct
     5101 tctcctgcag ctgccaatac cccaggccct gtgaatgccc gcgaacccag agcaggccca
     5161 cctcgccggc cgggacctcg accccgctgt catcggccgc cttcacttcg aatcccgcca
     5221 ccacctgccc gatcgtcccg ggcttcactg ccccgacgcg attggtcagg aagatgtgcc
     5281 acatctccgc ggtgccgagt ccgtcgagga gctccacgcc gaaggtctct ttccatcggt
     5341 ggtagagctc caccggcagc gcctctcccg cggacgtcgc gaatcgcaga ctcgagaggt
     5401 cctgcctggg ggcgtccggg tgactgacca tccggttcac catcgtcgga acgttgatca
     5461 gcacggtggg ccggtatttc gcgatgcggg tgaagagggc ctcaggcgta gacggttctt
     5521 cgaacagaat gacccgcgcg cccaccgaga atggaaagaa caggttggcg cccatcgcgt
     5581 acccgaagaa gagcttcggg acggacaccg tgatgtcgtc ggcacggtat cctaccgtgt
     5641 tcttggcgta gcactcggtc gtgttggcgt acgacccgtg ggtctgcacc accgctttgg
     5701 gctggccggt cgtcccaccc gagaagagcc agatggccgg gtcatcccgg tgcgtcggga
     5761 aggtttcgat cgaatcgggc agcgcttcca gacggagcgt cacgtcgggc gcatcgactt
     5821 tcagcacgcg cttcagggtg ggtgcgcccg cggccgccgc cacgaaggcc ggcgcagagg
     5881 gtcccgcgac caggaccgcg gtggcgcgcg tgtagtcgta gaagtacgcg atctgctccg
     5941 gcgtcagctg cgggttgacc atcactacca cggcgccaat cttgaggatg ccgaacagcg
     6001 cgcccacaaa tgccggaccg tctggcagcg cgatgatcac ccgttgctcg ggggagacct
     6061 gagcctcgcg caagagatgc gcgaagcggc tcgccagacg gtcgacttcc gcatacgtgt
     6121 acgttccctg atccgtcagc agcgccggcc gcgccccgag tccctcggca atccgggctc
     6181 ctagaaagta gtcggcgata ttgaaccgct cgggaggctc gaagaccata tgatggaagg
     6241 aatctggtgc cggttcgcga gctaggcgag ttgtcctgga ccgacatcca ggcactcgat
     6301 cggtcgcggg tcgtggccat ccttcctctg ggtgccgtcg aagcgcacgg accgcacctg
     6361 cccttgggca ccgacgtcat catcgccgaa gcgatggcgc gggccggcgc ggagctcctt
     6421 ctcccgcagg gtatccagcc actcctgctg ccggcgctgc cgtatgcgcc cgcgccctac
     6481 gccgcggact tccccggcac catctcgatc gacccgcacg cggtcacgtc gaccattcaa
     6541 gcgatcgcct cgagtctctc gcggcatgga atcaggttcc tggcactcgc caacgcgcat
     6601 ttcgatcccg tccaggtccg ggcggtccgg gacgccgtgc acggcaccga agccatggtg
     6661 atcttcccgg acctcacgcg ccgcgccctg gcccaacgcc tgacggcgga gttccagagc
     6721 ggcgcctgtc atgccggccg ctacgagacg tcgatcgtga tggcggcccg ccccgagctg
     6781 gttcatgaga ccctgcgcac cgccctgccg gcgaataacc ggtcactggt cgaagcgcac
     6841 gccgcggggc agacgacctt cagggccgcg ggcggcgccg atgcctattt cggtgctccg
     6901 gccgaggcgt ccgcgaccga aggccgggaa tcgattgcga aactggggga aatcctggcg
     6961 gacgccgtcc gtgaggtgat gcgatgagcg cggccgtcct ggccggcaaa cgcgcggtga
     7021 ttaccggcgg tggccgcgga atcggcgccg ccgtcgcgcg ggcgctcgcg gatgccgggg
     7081 tcggcgtgct cgtcgcggcg cggtcccgcg ccgaagtcga ggcggtggcg aacgcgctgc
     7141 gcgcgcaagg acaccgcgcc agcgccgcca ccgcggacgt caccaggccc gagagcgtca
     7201 acgccctcgc ccgcaccgcc accgaggtgc tcggcgacat cgacatcctg atcaacaacg
     7261 cgggatcgtc ctcgtcggca cccctgaagc ggacgacgct cgaggaatgg aaccgcctgc
     7321 tcgcggtcaa tgcgacgagt acgtttctct gcacccaggc ctttctcccc ggcatgctgg
     7381 accggaaatg gggccgggtg gtgaacctcg cctccgtcgc cggactcttc ggtgcgaagt
     7441 acatcgcggc ctatagcgcg gcgaaacacg cggtggtcgg gttcaccaaa tcgatggccg
     7501 ccgaggtcgc cggctcgggc gtgacggtca acgcggtgtg ccccggatat gtcgacacac
     7561 cgatgaccga ccaatcgctc gagcggatga tgcagaccac cgggaggtcg cgcgccgagg
     7621 ctctcgaggc ggtgctcgcg cattcgggcc aaagccggct gatcacgccc gacgaggtgg
     7681 cacgggccgt gatggcggta tgcaacggat cggagacggg tgcgacggtg cttctcgatg
     7741 gaaagggacc agcgtgagct tcgacgtcat caaccccgat ccccccggac ggcccaaggg
     7801 ctggaacaac ggcatgctgg ctcctgccgg gggccgcgtg ctcttcgtcg ccggtcagac
     7861 cgctccaacg gcgggtggac tcgtggctca gtggaccatg gcattgagtc aggttctgtc
     7921 gatcgtacgc gcctcgggcg gcggacccac cgacatcggc cggatgaccg tctacgtcac
     7981 gagcaaggcc gattacctgg ccaacctcca ggccctaggt gaagtccatc agaaggccat
     8041 gggcaagcat taccccgcga tggccttggt cgaggtctcc gccctcgtgg accctgcggc
     8101 cgtggtcgag atcgaagcca ccgcggtgat cggctgacca tggccatctt gcccaagtcc
     8161 ttcctctatg aagtcgattc cgggaccggt gtggcgacga tcacgctcaa ccggcccgac
     8221 cgcctgaatg ccctcacctt ccaggtctac gaggagctgc gggacgtctt ccgggctttg
     8281 gactcggagg cgggcgtcag ggccatcgtg atcaccggct cgggacgcgc gttctgctcc
     8341 ggcggtgacg tcgaggacat catcggccag ctcttctccc gggacgcgaa gggactgctg
     8401 gacttcacct ggctcacctg tgacctgatc ctcgccatgc ggctctgccg gcgccccatt
     8461 gtcgccgctc tgaatggcac cgtggccggc gcgggcgcgg tgatcgcggc cgcatgtgac
     8521 attcgcatcg cggcggagtc cgccaggatc gccttcctct tcacgcgggt cggcctttcg
     8581 ggcgcggaca tgggagccgc ctggttgctt ccccggctgg tgggcctcgg gcgggcgacc
     8641 gagctcctga tgacgggcga cttcatcgat gcgtcccgcg cgctggaaat cgggctctac
     8701 aaccgcgtgg tgcccgtcga tcgcgtggtg gcggaagcgc gggagttcgc ggcgcggctc
     8761 gcccttgggc cctcaaaggc actcgcggtc accaaggagg cactgaaccg cgaaggcgcg
     8821 atggatctgg aacaggcgct cgagctcgag gccaagttgc aagccgagct gatgcagcat
     8881 cccaacttcc gggaatcgta tgaggcgttc aaagccaagc ggcagccgaa atttcagtga
     8941 tgctccctga tccccggccc gtccgggcat tcctcgaccc gaagcacatc gacttcgcgg
     9001 cccgagtcgc cgagttcgcc gcgcgggaga tcgcgacgtt gccggcaccc gccgacgacc
     9061 ccgcggcgcg ggtgcaagcg aaggaaatcc tcactcgcct gggatccggg ggctggctcg
     9121 cgcccatcgg cgaccaggat tggcggtcgt gctgcctggt gcgggaagcc ctcggagccg
     9181 cgtcccccct cgcggatgcc gttttcgcgc tgcaggcgtt ggggaccctt ccgatcatga
     9241 tgggttccac cgacgatctg gcggctcgct ggctcacggc cgcgctccag ggacgcgcca
     9301 tgggaggctt cgccatgacg gagcccgagg ccggctcgga cgtcgcggcc atggccacca
     9361 cggcgcgtcg cgatggggca ttttacgtcc tcgacggggt caagaccctg atttccaatg
     9421 ccgggatcgc ggactactac gccgtgtatg ccgtaacgga tcggtcgaag gggaccaagg
     9481 ggatcacgtg cttcgtggtg cccgccgaca cgcccggttg ccgctttgtg cgcccattga
     9541 tcctctcctc gccacatccg ctgggagaaa tggccttcga gtcctgccgc atcccggccg
     9601 ggcagcgtct gggtgccgag ggcgagggat accgtctcgg actggcgacc ctcgaccgtt
     9661 tgcgcccgac ggttggcgcc gcggcatgcg gcatggcgtc gcgggccctg agcgaagcac
     9721 tcgaccatgc gacggcccga aaacagttcg ggagacccct cgccgacttt cagctcgtgc
     9781 aggaaaagct ttcgcgcatg gcgaccgacc tgaccgccgc gcgcctgctc gtatatcgcg
     9841 cggcgtggga gaaggatcat ggcgccgagc gcatcacgct cgaggcgggg atggcgaagg
     9901 ccttcgcgac cgaagctgcc cagcgcatca tcgacgacgc cgtgcagatc ctcggcggcc
     9961 gcggcgtgtt ggccgaccac cccgtggacc ggctctatcg gtccgtgcgc gctctccgga
    10021 tttacgaagg aacgacggag atccagcacc tggtgatctc cgggcagctc ctcaagcagc
    10081 atcgaggcga gtcgtgaaga tcgtctgcgt agggggcggc cccgccggcc tctacttctc
    10141 catcctgatg aagaaggccg acccttccca cgacattctc gtggtcgagc ggaaccggtt
    10201 cgaagacacc ttcggcttcg gcgtcgtgtt ctccgaggcc acccaggaga acctccaggc
    10261 cgccgatccc gagaccgtcg acgccatggc caaggcctcg gcgcactggg atgacatcga
    10321 catccactac aagggcggcg tcatccgctc gacgggccac ggcttctcgg ggctctcacg
    10381 ccgagcgatg ctcgagattc tggcccagcg ggcgcgcggg ttgggtgtgc aggtggagat
    10441 cggccgggac gtcaccgacc tcaaggacta cttgaaggcc gacctgctgc tcgccgccga
    10501 cggcgcgaac agcatggttc gggagcgtta cgccaagcac tttcagccgt ccgtcgacct
    10561 ccggcccaac cggttcgtct ggctcggcac cacccgtccc ttccccgcct tcacgttcta
    10621 cttcaagcac gaccggcacg ggctctggcg ggtgcatacc taccaatacg agaagggcca
    10681 ctcgacgttc atcgtcgagg cgcgcgagga gacctggcgc gccgcgtgcc tggaccgggc
    10741 cagcgaagag cagaccatcg cgttttgtga ggaattgttt gccgaggagc tcgccggcca
    10801 ccggcttcag aaaaaccggt ccatctggcg gcagttcggc accatccgga acgcgcactg
    10861 gcaccacgag aacgtcgtcc tgatcggcga ctcggcgcac accgcccact tctccgtcgg
    10921 ctccgggacc aagctcgcga tggaggacgc catcgttctc gcgcgctcgc tggccggcca
    10981 cgggaatcgg gatacagccc tgaacgcgta tgaagccgag cggcgtccgc tggtcgagag
    11041 tcttcagcgg gccgcccagg taagcctcgc ctggttcgaa gacaccgagc gctacatgga
    11101 catggacccg gtgcaattcg ggttcaatct cctcacccgg tcactccgca ttacccacga
    11161 caacctgaag gtgcgggatc cgaagttcgt ggcgggagtg gacgcgtggt tcgcgaaggg
    11221 cgcggccagg caatccggcg tcgcgattcc cgccgagccc gtgccgcccc cgctcttcac
    11281 gcccttccgg ctgcgtgagc tggttctcga aaaccgctcg gtcgtgtcac ccatgtgcca
    11341 gtactcggcg gaagacggca cgccgaatga ctggcacctc gtccatctgg gcacccgcgc
    11401 gattggtggc gcggcgctcg tgatgacgga gatgacggac gtcagccgcg agggccgcat
    11461 ctcgaccgga tgcaccggga tgtacaaacc cgaacacgtc gtggcgtggc gcaggatcgt
    11521 cgatttcgtc catggggcga gccgggcgaa gatcggcatg cagctgtcgc acgccggcag
    11581 gaaggggtcg acccggaaac tctgggaagg cgattcgctc ccgctcgagt cgggtgggtg
    11641 gcccatcatg gccgcctcag cgattcccta ctacccgaac agcccggtcc cgaaagagat
    11701 ggaccggcgg gacatggatc gggtacggga cgagttcgtg cagtccgcac ggatgaccga
    11761 ggaagcgggc ttcgatctac tcgagctgca cttcgcacac ggatacctgc tcgcgagctt
    11821 catctctccg ctcaccaacg tgcggaagga cgagtacggc ggcaccctcg aggcgcggat
    11881 gcgctatcca ctcgaggttt tcgacgccgt ccgcgcggtc tggcccaggc agaaacccat
    11941 gtcggtgcgc atctccgccg tggactgggc accgggcggc atggaatccg aagacgccgt
    12001 ccaggtcgcc cggctcctca aggcccacgg ctgtgacatc ctcgacgtct cggcgggtca
    12061 gacggtcccg ttccagtccc ccgtgtatgg ccgcctctac cagacgccct tcgcggatcg
    12121 gattcgacac gaggccgaaa tacccgtcat gacggtgggc aacatcagct cctacaccga
    12181 cgtgaacacc attctggccg ccggccgggc cgatctgtgc gtcctggcac gggctcatct
    12241 gtgggatcca tactggaccc gccacgcggc gcgcgagctg gggtgggaga tcgagtggcc
    12301 cgaccagtac gagagcttgg atcgctacaa gccccgcttt acgtaagggg cggctaccgg
    12361 cccgggccga tggcgttggg atccttcttg gtgaggacgc gctcggtgaa atcgaggcgc
    12421 tcgttcatct cggcgagatg cgattcgatc cggcccagct gatcctggaa tccgtccagc
    12481 tgaatcgccg tctgctcccc gctcagcccg ctcggctctc cgaacttgat gttggtcttc
    12541 cggcgaatgc gctcggccca cgcctccccc acgggggatt tcatgatcac gcggagtgcg
    12601 atgaggaacc ccacgcccac gatcaccgtc accacgatcg gtgccagcgt gttgttgatg
    12661 aagagattcc agtcgaacgg gatgtcggga atccctggtg gcggagtctg gatcatgttc
    12721 gtccctctac gcggcgccgc caggttcgtt tcacttcggc cagagccagc cgaacatccc
    12781 ggcggtcagg agcccgtaga tgaggccgtc caccgtgacc ttgatcgacg tcatcagggc
    12841 ccggttgtac cagatggtca tctgccacag tgccagcgtg tacgcgagga acgcggtcac
    12901 accgacgaaa cggaagaccg agagatacgg tgacccggcc ggcagcgccc gcccggcgac
    12961 gtaggccgcg aaaaacccga c
//
LOCUS       DASUBP010000197         9531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_986765, whole genome shotgun sequence.
ACCESSION   DASUBP010000197 DASUBP010000000
VERSION     DASUBP010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9531
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_986765"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="VFU75_07405"
     CDS             complement(<1..257)
                     /locus_tag="VFU75_07405"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HEU5466701.1"
                     /translation="MATTVVMEALSPTMEEGRLVKWSKKEGDQVKAGDTLAEVETDKA
                     VMELVARADGSLIKVVVQEGQTVPVGNVVAYIGAVGERVDGA"
     gene            complement(261..1241)
                     /locus_tag="VFU75_07410"
     CDS             complement(261..1241)
                     /locus_tag="VFU75_07410"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HEU5466702.1"
                     /translation="MPTLTYRDALNQALREEMQRDPNVFLMGEEVGLYQGAYKVSRGL
                     LEEFGPQRVVDTPIAELGFAGIGVGAAMTGLRPVVEFMTWNFAVLALDQIVNSAAKMN
                     YMSGGQFPMPIVFRGPNGAALQLGAQHSQAWESWLGHIPGLKVVAPATPADAKGLLKA
                     AIRDNNPVIVLEGEMLYNTKGDVPDGELVIPIGQADIKRPGKDVTLICHSKTVTVALK
                     AADQLQESGISAEVVDLRSIRPLDEAAIIASVTKTNRCVVVEEGWHHMGVGAQVVDII
                     QRDAFDHLDAPVLRVTQADVPMPYNKVLERAAKSSPEKVVAAARRVLYLD"
     gene            complement(1241..2248)
                     /gene="pdhA"
                     /locus_tag="VFU75_07415"
     CDS             complement(1241..2248)
                     /gene="pdhA"
                     /locus_tag="VFU75_07415"
                     /inference="COORDINATES: protein motif:HMM:TIGR03182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEU5466703.1"
                     /translation="MTPATAAQSKSKEKDALSPDRQRALLRQMLLIRRFEEKAAEAYA
                     LGKIGGFCHLYIGQEAVAVGSIAALKDDDYVICSYREHGQALVKGIAANAVMAELCGK
                     ATGCSHGKGGSMHLFDAKRHFMGGHGIVGGHIPLAAGIAFGIKYKGGNQVVLCYFGEA
                     AVNIGAFHEALNMAAVWKLPVVFICENNRYGMGTAFERVAALTDVAEHAKSYDMASAE
                     VDGMDVFAVYDATMKAVAAAREGKPTLLEVRTYRFMGHSMSDPLHGVYRTKEEVEEQK
                     KRDPIAALTHHLRDEGTMDQAAIDALDAEARAEVDAAAKFADQSPDPEPDALFHDVLA
                     D"
     gene            complement(2262..3185)
                     /gene="lipA"
                     /locus_tag="VFU75_07420"
     CDS             complement(2262..3185)
                     /gene="lipA"
                     /locus_tag="VFU75_07420"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HEU5466704.1"
                     /translation="MAAPLVQISPRQPAAPARKPSWLKVKAPGGQTYLGVQQMMRELK
                     LHTVCEEAHCPNIGECWEHKAATFMILGDVCTRNCAYCAVAHGTPAPFDPDEPRRLAD
                     AVAQMGLKHVVITSVDRDDLPNGGAESFASCITEIRQRLPESSVEVLIPDFKGNAEAL
                     RIVLHAKPDILNHNLETIDRLYRIARPGGRYTRALELLARAKEIAPDQLTKSGIMVGL
                     GEDWDELLVSMRDLRAQRVDILTLGQYLRPSADHLPIARWYTPEEFAELKRLGMEMGF
                     RHVESGPLVRSSYHAWEQAERAAATTAVAPR"
     gene            complement(3234..4235)
                     /locus_tag="VFU75_07425"
     CDS             complement(3234..4235)
                     /locus_tag="VFU75_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466705.1"
                     /translation="MYQSCAFCNGKFTGDGGPSGLGVGRRFAYDEWRGRLWVICTSCS
                     RWNLAPIDNRLDRIEAVARAAAGGMVAASTEQVSLIRSGTYDYVRVGKPPRIEYATWR
                     YGERIKARRREQLKYVVPLSIGAVGAAIAINVAAGGSVGIFVWQIPQIAKSLYTRAVG
                     SRPVPLSETPVCARCGNVMKVRARDLGRARLVANSHAQLVLLLTCGKCESEGAQLEGV
                     DAQMALRRGLTYQNLARGGRQRAADAARIVDRAGGPDQLVNGMARESLLLRTLRPDRG
                     LALEMAVDEQAEVEELERQWRIAEETAAIADGTLSTDAEIEEELKRLKGRPPNQPNS"
     gene            complement(4242..5363)
                     /gene="thiO"
                     /locus_tag="VFU75_07430"
     CDS             complement(4242..5363)
                     /gene="thiO"
                     /locus_tag="VFU75_07430"
                     /EC_number="1.4.3.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016647 - oxidoreductase activity, acting
                     on the CH-NH group of donors, oxygen as acceptor [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine oxidase ThiO"
                     /protein_id="HEU5466706.1"
                     /translation="MSAPVSPDVAVIGGGVVGAACARAAALRGLRTVLYEPGPDPAAA
                     SPASAGMLAAQIEPDDEALLALAIRGRETYEPLARSLKDTTGLDVGFWREGIASVAFD
                     EAGMKRLRDMAAWQRQMGLSCRWLESREALELWPALSPDALGALLAAEDGAIDPPLLT
                     NALRTDAERLGVSPSRESVTGIVVEDGRATGVRTVAGTAPAGMVVIAAGAWSGRLAGL
                     PRPLPVEPVRGQLALAPWPADTPPAILYYDHAYVLARGGKAIIGSTMERVGFDARVTR
                     EGEEQLRAGAARLFPSFATLPFEKTWAGLRPLTPDGRPILGRDPLVSGLWYAVGHGRN
                     GILLAALTGDVIGDLMIGKQLDFDLEPFLIDRFSPTPDP"
     gene            5409..5624
                     /locus_tag="VFU75_07435"
     CDS             5409..5624
                     /locus_tag="VFU75_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466707.1"
                     /translation="MFRSILGFAVLAVVAWIALKIFFGLLSGIMGLAITVLWLAAIGF
                     VCYLVLRVIAPRTADRVREAIKGRPSA"
     gene            complement(5695..6642)
                     /locus_tag="VFU75_07440"
     CDS             complement(5695..6642)
                     /locus_tag="VFU75_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466708.1"
                     /translation="MTEYREADLSRLTTVPIAGRRNKVDSTLLAHPPGADRSFDAFWK
                     SLPDILAARDLRFVADRVVQAAGKKAVVVMMGGHVIKVGLGPLLITLMKRGVITHLAM
                     NGSAAIHDFELAAFGGTSEDVAAGLKDGTFGMAEETGKEMNAAISAGAKKDWGAGEAL
                     GHALKDRKGSPGREVSVLLAAHELGIPCTVHAALGAEITHQHPTADGAAIGATSHRDF
                     KRLAGALPALDQGGVVLNLGSAVLMPEVFLKALTIARNLNGGKPTGFTAVDCDMQRHY
                     RPRVNVVERPTLEGGRGVQLTGHHEVMLPMLVWRVLESL"
     gene            complement(6639..8126)
                     /locus_tag="VFU75_07445"
     CDS             complement(6639..8126)
                     /locus_tag="VFU75_07445"
                     /inference="COORDINATES: protein motif:HMM:NF022750.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HEU5466709.1"
                     /translation="MIYVCVPVHNEARTAGLVLWKVRQVFTGFSREYQILACDDASTD
                     DTAEVLARYARVLPMTVITNTQRQGYAKSLDALLKAALQRTDRPKRDCAITLHADFVH
                     SPDAMEEMVKRLESGADLVVGEQKQLAGPVTRGFRWCRALAPRLLRVPGVRDTVSGFL
                     ALRLVVLRQALKPGAESLLSTDGWSANAELVARLARNSRKLERVDYAARYDLRQRRSR
                     TTGWDELMRAWRARPVIRAARMAMLAAFALCAAPRAGAQNTPATDSDAAPATRPVPFP
                     VGEHLVFGAKYGFISVGTATMDVVGIDTVRGTPTVHLLFHIIGSALFGAYKLDQRLES
                     WVGKDDFVSRRYRIDTDEKGKKWGHTYDIFPDSGYYRTDGTDTTHASVPNPLDDAAFL
                     YWIRTVPLEDGKKYTYRRYFRPENDPIIVEVVGHDRVSVAGKKWDAIVVRPAIPNGRG
                     IFAEKADARIWLSDDSTRTLLALQSTFSFGQVTLKLKEFSVPERK"
     gene            complement(8138..9223)
                     /locus_tag="VFU75_07450"
     CDS             complement(8138..9223)
                     /locus_tag="VFU75_07450"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5466710.1"
                     /translation="MRVLQVDSGREWRGGQNQVRLLCRELTHQRDVENRLFTSRGSEL
                     ALRVKGSGGSVVQARWNSGLSLTAFLALGQAVGALRPDIVHVHDSHALTLALWLRKTG
                     PNWIARLISPGMVKWQPYTIVAHRRVDFHVRPGSGWHQADRVIAVSEAVKRVLVADGI
                     DSRDVVVVPDGIDPYEIGAAALGAPDIRKRLGLPADTPLAVNVAALVDHKDQRTLIRA
                     AASARAGAPRLHWVIAGEGPLRADLQREIANLGVGDIVHLVGYVEPVDALIKESSVFV
                     MSSKEEGMGSVVLHALALEKPVVATAAGGLPEVVAPEWLAPVGDADALAAKTLLALER
                     APRTSLPERFTAKAMAEAILAQYRVLA"
     gene            complement(9256..>9531)
                     /locus_tag="VFU75_07455"
     CDS             complement(9256..>9531)
                     /locus_tag="VFU75_07455"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5466711.1"
                     /translation="SDGSDAVDRAVAQPFDVIVCDLRMPRLGGREMYEKLAEQHPEVA
                     DRVIFATGDTVRGDTLSFLESLGRPFLHKPFTLAELRTVLGGVRRAG"
BASE COUNT         1532 a         3165 c         3197 g         1637 t
ORIGIN      
        1 gccccatcga ccctctcccc cacggccccg atataggcca ccacattgcc gacggggacc
       61 gtctggcctt cctgcaccac gaccttgatg agggagccgt cggcgcgcgc caccagctcc
      121 atcaccgcct tgtcggtctc gacctcggcg agggtgtcac ctgctttgac ctggtcgccc
      181 tccttcttcg accatttgac gaggcgcccc tcttccatcg tgggcgagag cgcttccatc
      241 acgactgtgg tcgccatggg tcagtccagg tacaggacgc gccgcgccgc ggccacgacc
      301 ttttcgggag aagacttcgc ggcgcgctcg agcaccttgt tgtacggcat cggcacgtcc
      361 gcctgggtca cgcgcagcac cggggcgtcg aggtgatcga aggcgtcgcg ctggatgatg
      421 tcgaccacct gcgccccgac acccatgtgg tgccagccct cttcgaccac gacgcagcgg
      481 ttggtcttcg tcaccgacgc gatgatcgcg gcctcgtcca gggggcggat ggaccggaga
      541 tccaccacct cggcggagat acccgattcc tgaagctggt cggcggcctt gagcgcgacg
      601 gtgacggtct tcgaatggca gatcagggtg acgtccttgc cggggcgctt gatgtcggcc
      661 tggccgatcg gaatcacgag ctccccgtcg ggcacgtcgc ccttcgtgtt gtagagcatc
      721 tcgccctcca gcacgatgac ggggttgttg tcacggatcg ccgccttgag caggcctttg
      781 gcgtccgccg gcgtggcggg tgcgacgacc ttgaggccgg ggatgtgccc gagccacgat
      841 tcccacgcct gcgagtgctg ggcgcccagc tgcagcgcgg cgccgttggg accgcggaag
      901 acgatcggca tcgggaactg gccgcccgac atgtagttca tcttggcggc ggagttcacg
      961 atctggtcga gcgcgaggac ggcgaagttc caggtcatga actccaccac cggccggagc
     1021 cccgtcatcg ccgcaccgac gccgatgccg gcaaatccca gctcggcgat gggggtatcc
     1081 accacccgct ggggaccgaa ctcttccagc aggccgcggc tcaccttgta cgcgccctgg
     1141 tagagtccca cctcttcgcc catcaggaac acgttgggat cccgctgcat ctcttcgcgg
     1201 agcgcctggt tcagggcgtc gcggtacgtg agtgtgggca tcagtcggcc agcacgtcgt
     1261 ggaagagggc gtcgggctcg ggatcgggac tctggtccgc gaatttcgcg gccgcgtcta
     1321 cttccgcgcg tgcctcggcg tcgagggcgt cgattgccgc ctgatccatg gttccctcat
     1381 cgcggagatg atgggtcagg gccgcgatcg ggtcgcgttt cttctgctct tccacttctt
     1441 ccttggtccg gtagaccccg tgcagggggt cggacatcga atggcccatg aagcggtagg
     1501 tgcggacttc gagcagcgtg ggcttgccct cgcgcgccgc ggccaccgcc ttcatggtcg
     1561 cgtcgtatac cgcgaagacg tccatgccgt ccacctcggc cgacgccatg tcgtagctct
     1621 tcgcgtgttc ggcgacgtcg gtgagggcgg cgacccgctc gaacgcggtg cccatgccgt
     1681 aacggttgtt ctcgcagatg aagacgacgg ggagtttcca gacggcggcc atgttcaggg
     1741 cctcgtggaa ggcgccgatg ttgacggccg cctcgccgaa atagcagagc acgacctgat
     1801 tgccgccctt gtacttgatg ccgaacgcga tgcccgccgc gagagggatg tgcccgccga
     1861 cgatgccgtg gccacccatg aagtggcgct tggcgtcgaa caggtgcatc gagccaccct
     1921 tgccgtgcga gcaccccgtc gccttgccgc acagctcggc catcacggcg ttggccgcga
     1981 tgccctttac cagcgcctgg ccatgttcgc ggtaggagca gatgacgtag tcatcgtcct
     2041 tgagggcggc gatggaaccc accgccaccg cctcctggcc gatgtagagg tgacagaacc
     2101 cgccgatctt gccgagcgcg tacgcttcgg cggccttctc ctcgaaccgg cggatcagga
     2161 gcatctggcg gagcagggcg cgctgccggt cgggcgacag cgcgtccttc tctttggatt
     2221 tggattgtgc ggcagtggcg ggggtcatgt ctctttcgga gttagcgggg ggcgacggcc
     2281 gtcgtcgcag ccgcgcgttc ggcctgttcc caggcgtgat aactcgagcg caccaggggc
     2341 ccggactcca cgtgccggaa tcccatctcc atgccgaggc gcttcagctc ggcgaactcc
     2401 tcgggggtgt accagcgggc gatcgggagg tgatcggccg agggccggag gtactggccg
     2461 agggtcagga tgtcgacgcg ctgagcgcga aggtcgcgca tcgacaccag cagctcgtcc
     2521 cagtcttcgc ccagtcccac catgatgccc gacttggtca gctggtcggg agcgatctcc
     2581 ttggcgcggg cgagcagttc cagggcgcgg gtgtagcggc cgccgggccg cgcgatgcgg
     2641 tagaggcggt cgatcgtttc caggttgtga ttgaggatgt cgggcttggc gtggaggacg
     2701 atccgcagcg cttccgcgtt gcccttgaaa tcgggaatca ggacttcgac ggaggattcg
     2761 gggagccgct gccggatctc cgtgatgcac gaggcgaagc tctcggcacc cccgttcggc
     2821 aagtcatcgc gatcgacgct cgtgatgacc acgtgcttca ggcccatctg tgccacagcg
     2881 tccgccagcc ggcgcggctc gtccggatcg aaaggcgccg gcgtcccgtg ggccacggcg
     2941 cagtaagcac agttccgggt gcacacgtcg ccgaggatca tgaaggtggc ggccttgtgc
     3001 tcccagcatt cgccgatgtt ggggcagtgg gcttcttcgc agacggtgtg gagcttgagc
     3061 tcccgcatca tctgctggac gcccaaatag gtctgtcccc cgggagcttt gactttcagc
     3121 caggaaggct tgcgcgccgg ggctgcgggc tgcctggggc tgatttgtac gagcggagcg
     3181 gccatatggt ggcgggaaga tagtgccgtc aggtccttgg agatagtgca aaatcaacta
     3241 tttggttgat ttggcggtcg ccctttgaga cgcttcaact cttcctcgat ttcggcgtca
     3301 gtgctgaggg tgccgtcggc gatggccgcc gtttcctcgg cgatccgcca ctgccgctcg
     3361 agctcttcca cctcggcctg ttcgtcgacg gccatctcca gcgccagccc ccggtcggga
     3421 cgcagcgtcc tcagcaacaa ggactcccgg gccatgccgt tgaccagttg atcgggccca
     3481 cccgcccggt ccacgatgcg ggcggcatcg gcggcacgct gcctcccgcc ccgtgcgagg
     3541 ttctggtacg tgagtccccg cctcagcgcc atttgggcgt cgacaccctc gagctgcgcc
     3601 ccctcactct cgcatttgcc gcaggtcagc agcagcacca attgggcgtg ggaattcgcg
     3661 acgaggcggg cccgccccag atccctcgcg cggaccttca tcacgttgcc gcaccgggcg
     3721 cataccggcg tttcggatag aggcaccggc ctggatccca ccgccctggt gtacagggac
     3781 ttggcgatct gcggaatttg ccacacgaag atcccgaccg agccacccgc cgcgacattg
     3841 atggcgatcg cggcgccgac cgcgccgatg ctgaggggca caacgtactt cagttgctca
     3901 cgacggcggg ccttgatgcg ttccccgtac cgccaggtcg cgtactcgat ccgcggcggc
     3961 ttgccgaccc gcacgtagtc atacgttccg gacctgatga gcgagacctg ctcggtagag
     4021 gcggccacca ttccaccagc cgccgcgcgg gcgacggcct cgatgcggtc caggcggttg
     4081 tcgatcggcg cgaggttcca gcggctgcac gacgtacaga tgacccagag ccgcccgcgc
     4141 cattcgtcgt acgcgaagcg gcggcccacg ccgagtccgg aggggccgcc atcgccggtg
     4201 aacttcccgt tgcagaaggc gcaagactga tacatcaggg atcaggggtc gggggtcggg
     4261 gaaaagcgat cgatcaagaa cggctcaaga tcaaaatcca actgcttccc gatcatgaga
     4321 tccccgatca catcgccggt cagagccgcg aggaggatgc cgttccggcc gtggcccacg
     4381 gcgtaccaaa gacccgacac gagcggatct cggcccagaa tcgggcgtcc gtccggcgtg
     4441 agtggccgga ggccggccca ggtcttctcg aacggcaacg tcgcgaagga cgggaacagg
     4501 cgcgccgcgc ctgcccgcag ctgctcctcg ccctccctgg tcactcgagc gtcgaatccg
     4561 acgcgctcca tcgtggaccc gatgatcgcc ttgcccccgc gggccaggac gtaggcgtgg
     4621 tcgtagtaca ggatggcggg aggtgtgtcg gcgggccacg gagcgagggc gagctggccc
     4681 cgaaccggct cgacgggcaa ggggcgaggc aaaccggcga gtcgtccgga ccacgcaccc
     4741 gcggcaatca ccaccatgcc cgccggcgcc gtgcccgcca ccgttcgaac cccggtggcg
     4801 cgtccatctt ccacgacgat gccggtcacc gactcccgtg agggactcac gccgagccgt
     4861 tcggcatccg tgcgaagcgc gttcgtgagg agaggcgggt cgatcgcgcc atcctcggcg
     4921 gcgagcagcg caccgagggc gtcgggggag agggcgggcc agagctccag ggcttcccgc
     4981 gattcgagcc accggcacga cagccccatt tgtcgctgcc acgccgccat gtcgcgaagt
     5041 ctcttcatcc cggcttcatc gaaagcaacg gatgcaatac cctcccgcca gaacccgacg
     5101 tccaacccgg tggtatcttt gagagagcgg gcgagtggtt cgtaggtttc tcgcccccgg
     5161 atggcgaggg cgaggagtgc ttcatcatcc ggctcgatct gcgcggcgag catgccggcg
     5221 gaggccggag aggccgccgc gggatcgggg cccggttcat agagcaccgt ccggagaccg
     5281 cggagcgcgg cggcccgggc gcaggccgcg cccactacgc cgccaccgat cacggccaca
     5341 tcgggtgaaa ccggagcgct catcgcgccg tatacttgga agcggatcgg aacttcacgg
     5401 aggacaccat gttccggagc atattggggt ttgcggtact cgccgtcgtc gcctggatcg
     5461 cgctcaagat cttcttcggg ctcctgtccg gaatcatggg tctggccatc accgtgctct
     5521 ggctggccgc catcggattc gtctgttacc tcgtgctgcg ggtcattgcg ccgcgcacgg
     5581 cggaccgggt gcgcgaagcc atcaaaggcc gcccgtcagc ctagactgca gccgtcagcc
     5641 gtcagccatc agcgatacag tttgctgatg gctgattgct gatagctgat ggcttcacag
     5701 gctctccagg accctccaga ccaacatcgg cagcatcacc tcatggtgtc cagtcaattg
     5761 gaccccacgt ccgccctcca gcgtcggtct ttccaccaca ttgacgcgcg gccggtagtg
     5821 ccgctgcatg tcgcagtcga cggcggtaaa gccggtcggt tttccgccgt tcaagttgcg
     5881 ggcgatggtc aatgccttca ggaacacttc gggcatcaac accgcgcttc ccagattcag
     5941 tacgacgccg ccctgatcga gcgcagggag cgcccccgcc agtcgcttga agtcgcggtg
     6001 gctggtggcc ccgatggctg ccccatccgc cgtggggtgc tgatgcgtga tctccgcgcc
     6061 gagtgcggcg tggacggtgc aggggatgcc gagctcgtgg gcggcgagca gcacggagac
     6121 ctcccgtccg ggtgatccct tccggtcctt cagcgcgtgc cccagggctt ctccggcgcc
     6181 ccagtccttc ttcgcgcccg ccgagatcgc ggcattcatc tccttgccgg tctcttccgc
     6241 catcccgaag gtcccgtcct tcagtcccgc cgcgacatct tcgctggtgc cgccgaacgc
     6301 ggccagctcg aagtcgtgga tggcggcgct gccgttcatg gcgaggtgcg tgatcacgcc
     6361 ccgcttcatg agggtgatca gcaggggccc gagtccgacc ttgatcacgt ggccgcccat
     6421 catcaccacg accgccttct tgcccgcggc ctgcacgacg cggtcggcga cgaaccgcag
     6481 atccctggcg gcgaggatgt cgggcaggga cttccagaac gcgtcgaacg agcggtcggc
     6541 gccgggggga tgagcgagaa gagtggaatc gactttgttg cggcggccgg cgatgggaac
     6601 cgtggtgagg cgggagaggt ccgcctcgcg gtactcggtc atttgcgctc cgggacggag
     6661 aactccttga gcttcagggt gacctggccg aacgagaacg tcgattgcaa ggcaagcaaa
     6721 gtgcgggtgg agtcgtcgga cagccagatg cgggcatccg ccttctccgc aaagatcccc
     6781 cggccgttcg ggatggcggg gcgcaccacg atggcgtccc acttcttccc cgcgacgctc
     6841 acgcggtcgt gcccgaccac ttcgacgatg atggggtcgt tctccggacg gaagtaccgg
     6901 cggtacgtgt acttcttccc gtcctcgagg ggaacggtgc ggatccagta caggaaggcc
     6961 gcatcatcga gcggattcgg gaccgacgcg tgcgtcgtat cggtgccgtc cgtccggtag
     7021 taaccggagt cggggaaaat gtcgtacgtg tgtccccact tcttgccctt ctcgtcggtg
     7081 tcgatccggt accggcggga cacgaagtca tccttcccca cccacgactc gagcctctgg
     7141 tccagcttgt aggctccgaa gagcgcactg ccgatgatgt gaaagagcag gtgtacggtc
     7201 ggggtgcccc ggactgtgtc gatgccgacg acgtccatgg tggcggtgcc gacgctgatg
     7261 aagccgtatt tggcaccgaa gaccaggtgc tcaccgacgg ggaagggcac ggggcgcgtg
     7321 gcgggggcgg cgtccgaatc cgtggccgga gtattctggg cgccggcccg aggcgcggcg
     7381 cagagcgcga aggcggccag catggccatt cgcgcggcgc gaatcaccgg gcgagcgcgc
     7441 caggctcgca tcagctcgtc ccagcccgtg gtccgggacc ggcgctgccg cagatcgtag
     7501 cgggcggcgt agtccacccg ctcgagcttc cgggaattcc ttgccagccg cgccaccagc
     7561 tcggcgttgg ctgaccagcc gtcggtagag agcagggact ccgcgccggg cttgagagct
     7621 tggcgcagca cgacgagacg cagggccaga aatcccgaca ccgtatcccg cacgccgggg
     7681 actcgcagga gcctcggcgc caacgcccga caccaccgga agccacgagt caccgggccg
     7741 gccaattgct tttgctcgcc gaccaccaga tccgcgccgg actcgaggcg cttcaccatc
     7801 tcttccatgg cgtcggggct gtggacgaag tcggcgtgga gcgtgatggc gcagtcccgt
     7861 ttgggacgat cggtgcgttg cagtgcggcc ttgagcagcg catcgagact cttcgcgtaa
     7921 ccctggcgct gggtgttggt gatgacggtc atcggcaaca cgcgcgcgta ccgggcgagc
     7981 acctccgcgg tatcgtccgt cgacgcgtcg tcgcaggcga ggatctggta ttcccgggaa
     8041 aatccggtga acacctgtcg gaccttccac aggaccagcc cggcagtgcg ggcctcattg
     8101 tgcacgggga cgcagacgta aatcatggtg agggaatcta ggctaggacc cggtactgag
     8161 ctaagatggc ctctgccatc gccttagctg tgaaccgttc gggcaaggag gtgcgcggcg
     8221 cccgttccag cgcgagcagc gtcttcgccg cgagcgcgtc ggcgtcgcca accggggcga
     8281 gccactcggg ggccacgact tcgggcagac cgccggccgc cgtcgccact accggctttt
     8341 ccagggcgag cgcgtgcagc acgacgcttc ccatcccttc ttccttggat gacatcacga
     8401 acacactcga ctccttgatc agggcgtcca ccggttcgac ataccccacc aggtgaacga
     8461 tgtcccccac gcccaggttc gcgatctcgc gttgcagatc cgcccgcagt ggcccctctc
     8521 ccgcgatcac ccaatggagt cgcggcgcac cggcacgcgc agaggcagcg gcgcggatga
     8581 gcgtgcgttg gtccttgtgg tcgacgaggg cggcgacgtt gacggcgagc ggtgtgtcgg
     8641 cgggaaggcc caaacgcttt cgtatgtcgg gcgcaccgag ggcagccgcg ccgatctcgt
     8701 aggggtcgat gccgtcggga acgacgacga catcccgcga atcgatcccg tcagccacca
     8761 ggacgcgctt cactgcttcg gacaccgcga taacccgatc cgcctggtgc catccgctcc
     8821 cgggccgcac gtgaaaatcc acgcgacgat gcgcgacgat ggtgtacggc tgccacttga
     8881 ccatgccggg tgaaatgagg cgcgcaatcc aattgggtcc ggtcttgcgc agccacagcg
     8941 cgagggtgag cgcatgactg tcatgaacgt gcacgatgtc cggccggagg gcgccgacgg
     9001 cttgacccaa ggcaaggaat gcggtcagcg ataggccgga attccatcga gcctgcacga
     9061 cggagccgcc ggagccctta acccgaagcg cgagctcgga tcccctcgag gtgaacagtc
     9121 tgttctcgac gtcgcgctgg tgggtcagtt cccggcagag caggcggacc tgattctgtc
     9181 ccccgcgcca ctcccgcccg gagtcaactt ggagtactcg cactgtctaa ccgtctagcc
     9241 gtctagtcgt aagcatcagc cggcgcggcg cacgcccccg agtacggtgc gcagctcggc
     9301 gagcgtgaag ggcttgtgga gaaaaggacg gccgagggat tcgaggaagg acaaggtgtc
     9361 gccgcgaacg gtgtcgcccg tcgcgaaaat cacccggtcg gccacttcgg gatgttgttc
     9421 cgcgagcttc tcgtacattt cgcggccacc caggcggggc atgcggagat cgcacacgat
     9481 gacgtcgaac ggctgggcga cggcccggtc cacggcgtca ctgccatcgg a
//
LOCUS       DASUBP010000198         8864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_495892, whole genome shotgun sequence.
ACCESSION   DASUBP010000198 DASUBP010000000
VERSION     DASUBP010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8864
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495892"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1280
                     /locus_tag="VFU75_07460"
     CDS             <1..1280
                     /locus_tag="VFU75_07460"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEU5466712.1"
                     /translation="IPFADFQTLVSRELGRPLDDLFLRVDPEPLGSASIAQIHRATTR
                     AGDPVVVKAVKPGIVETLRRDALLLRILGAVLEWPFGRYQPRRIIREFVAYTLREVDL
                     KQEAENARAFAANFADLAGVAFPVVYPEYSGARVLCMEFVDGLRPDTAAARALPARAR
                     RQLVELGAESIIRMIYQDGFFHADLHPGNLLILPGARIGFIDLGMVGRLDAEVRRLML
                     YYYYSLVTGDAENAARYLTAVAQPGRRGDPQGFRREVTELSHRWSRTATFEGFSLAQL
                     ILESVSRGGKYRMYFPVELVLMVKALVTFEGVGHMLLPGFDVAEVSRRYIREIFLAQF
                     SPLRLVQEELRGAPDLMDAAIKIPLLVTEGVRVLERNARRPAENPLAGVRGTLIAGFC
                     LVAGAILMAFGGPWPLWVALFGAALALAVRRGP"
     gene            1282..2490
                     /locus_tag="VFU75_07465"
     CDS             1282..2490
                     /locus_tag="VFU75_07465"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEU5466713.1"
                     /translation="MAAPPVGAPATDRHSRRLRLWYDPFDPEYCRDLLDRTLGPVARQ
                     YFRPRLIGAERLPARGPAILAANHSGNAFPYDAIMLDATLWRADEFAVARKVRSLFEP
                     ALTESWWMRPFGIDNFWRRGGGVDMTFDNFERLMQRGDRVLYFPEGVPGIGKGFNRRY
                     QLQPFKTSFVVMAARHGAPVYPISIVNGEWIVPFGYTFRFLDGIMQRVFRVPFLPIPI
                     GLLGVVFPFMWYFAFPAHLVFVVGRPIDVRAMMWREGITDFESPPRAAARRVAERIRV
                     LMQQELDAEVALHGRRPFEVASLWKAVVGGTLSFGRVLPPAWTLTMVRQDRDRHRPAP
                     RNRLLGLLRDWDLLFFYVPFGWPLLSLARRLRRPPYGYRGLHPEERRDVQGQFLWRLV
                     DRPLPPHRQP"
     gene            complement(2481..3707)
                     /locus_tag="VFU75_07470"
     CDS             complement(2481..3707)
                     /locus_tag="VFU75_07470"
                     /inference="COORDINATES: protein motif:HMM:NF013862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEU5466714.1"
                     /translation="MAGARRTPRRHIGLALAGGGPAGAVYEIGALYALEECLEGLDFA
                     DLSIYVGVSAGAFLTAALANGVKPSELARTVVRSEGAESPFSPGTFFKPALRGWARQG
                     ARLPKLFATALSRVAVHPGDPSVRESLSRLAGGLPVAIFENEPIRRYLARMLSQPGRT
                     DDFRQLKRKLVIVATDLGSGRAIRFGEPGFDHVPISRAVQASSAFPGLYPPVTIDGRP
                     CVDGVLLKTLHASVALEDGAGLVLCVNPLVPADITTGIATGALRPDALTAGGLVTVLS
                     QTVRTMIHSRLEVGMSAYGTRYPDSDVVLFEPHPDDYGMFFTNIFSFSARGAVCELAY
                     QSTRRTLRQRRAEIETALARHGITLRGDVLDDTTRTMSTEGAPLATGGGAGGAAGATR
                     TLRNTLARLADSLRQG"
     gene            complement(3717..4130)
                     /locus_tag="VFU75_07475"
     CDS             complement(3717..4130)
                     /locus_tag="VFU75_07475"
                     /inference="COORDINATES: protein motif:HMM:NF017415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HEU5466715.1"
                     /translation="MAKVIQHKRMPLVRQSAQQVWLAGLGALALAEEEGGKLFGSLVK
                     KGHTVDKANRQKVGKALARVNKIRENARRSVGRFRLPVDTGMAGALHRFGIPSRQEIV
                     TLTKRVEELTRSVERAKARPRKRAPAKHVVAAAAV"
     gene            4273..4830
                     /gene="efp"
                     /locus_tag="VFU75_07480"
     CDS             4273..4830
                     /gene="efp"
                     /locus_tag="VFU75_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEU5466716.1"
                     /translation="MKATDIRRGHVIMMDGNPCRVEEFTHRTPGNLRAFVQLRLRNLN
                     SGNTFDTRVSATDFLDEVRLDQKDFQVLYRDESGVHVMDAENYEQYTLDKGAVGDQGD
                     WLQPEMRFQAEWLDGKPIGIKLPAVMELDVVETSPVMKSATKTASSKPAKLSNGVTIQ
                     VPEFIATGDRLRVDPREGKYLERAK"
     gene            4936..8262
                     /locus_tag="VFU75_07485"
     CDS             4936..8262
                     /locus_tag="VFU75_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466717.1"
                     /translation="MHRPGVLPFLTLAGLVSLATPLAAQAKPNRKPAAKAPADATATA
                     SAAPAASPEDSLNTIRFRNLGPSVAGGRVSTVVGIPGQPGSYYVGAGAGGVWKTTDNG
                     NTWEAIFKDQPAASIGAVALAPSNPNLVWVGTGEAAIRNDVVNGHGVWFSPDAGHSWR
                     FMGLGDVGQISKIVVDPANPDVVLVAALGHAWSPNGDRGIFRTTDGGKTWQKTLFVND
                     TTGASDLIMDPGNPRVYFAGMWQFVRHPWELTSGGAGSGIYRSTDGGVTWERLSQGLP
                     RGIYGRIALGIGASNPSHIYALVEAKTGMLWESHDLGDHWSSVSDNHALDVRPFYFSQ
                     VTVSPIDDRKLYFSSYELLESADGGKTAKAIDQGVHVDHHALWIDPTNAQRIIQGNDG
                     GAYATTDGGKNWTWFNNLPIEQFYMVAATSGDPYMICGGLQDNNGWCGPVGASDGAQW
                     FTVTGGDGEYVVPAPSDSTILYVDSQNGSISRVNLRTGESHGIRPYEPSVEGTKPSEL
                     TYRFNWTSPIEVSATNADEVFIGGNVVFKSTDGGQHWVPISPDLTRNDKTKQVNSGGP
                     IEYDISGAETYNTILTVNVAPTDTNVIWVGTDDGLVQVTKDGGKTWTNVGGHFPGLAA
                     DEEGRVYQVGISPFDAGTAYVTYDRHEFDDNRAYAYKTADYGKSWTRIDAGLPEYAAH
                     VVREDPNARNFLVLGTDQGLWFSRNGGASWKAMKADFPTTPVYDVKFVKASHDLLVAT
                     HGRGLFALDNINSLEGLTAEVAAQDFHLFPVVPAVLRGRARGRSGVAPTNFTVRGAPQ
                     GAVIDYYLKAGVDTTSPAGSPEAGGPGGGRGGRGGPGGFGGGRRSGARLVVTSAAGDT
                     VFVDSSGPTKMGVNRMTWGLRWNGPTPLTIEPPQGGEGGGGFRRGGFGPAVVPGTYTV
                     TVSVAGKSGSQTVEVKPDPVQGGDPAKFQAQLAAGLQFRSELTALHEMLNHLVAIETQ
                     IRNAQTAVRETGDTAAMGALNRASRDLSRKLRQLKDTLYNSDQQRGAPEDDIHYLTRF
                     ADKYQSLGFGVFGGYAQPPSPQLMDEWKVERAQLDGFIATFNGILNTDVAAYNQVANA
                     NHTPVLLGGGPIAIKAASAR"
     gene            complement(8251..8637)
                     /locus_tag="VFU75_07490"
     CDS             complement(8251..8637)
                     /locus_tag="VFU75_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5466718.1"
                     /translation="MAKTKKARVLIIDDDRPLCELFAAHLRGAGYVPMIAEDAMQGFM
                     FAQRELPDVILLDINMPAGGGMTVFDRLNKSAKTREIPVVVVTAVHDAKVDADSTARG
                     ARAVLHKPIDKDTLLKQVATVLESAG"
     gene            complement(8648..>8864)
                     /locus_tag="VFU75_07495"
     CDS             complement(8648..>8864)
                     /locus_tag="VFU75_07495"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEU5466719.1"
                     /translation="LLWDCVGGAPIKNARRAQRGLPAETRESRRMSDILSERGFKFVG
                     PTICYAFMQAVGMVNDHLVTCFRHGEV"
BASE COUNT         1430 a         2901 c         2990 g         1543 t
ORIGIN      
        1 ccatcccgtt cgcggacttc cagacgctgg tctcccgcga gctcggccgc ccgctcgacg
       61 atctgttcct ccgcgtggat ccggaacccc tcgggtcggc gtccatcgcc cagattcatc
      121 gggcgacgac gcgcgcgggg gacccggtcg tggtcaaggc ggtgaagccg ggcatcgtcg
      181 agacgttgcg acgggatgcc ctgctgctcc gcatcctcgg cgccgtgctg gagtggccgt
      241 tcggccgcta tcaaccacgc cgcatcatcc gggagttcgt cgcatacacc ctgcgagaag
      301 tggacctgaa gcaggaggcg gagaacgcgc gcgccttcgc cgcgaacttc gcggacctcg
      361 cgggcgtcgc tttcccggtt gtgtatccgg agtactcggg cgcccgggta ctgtgcatgg
      421 aattcgtcga tgggttgcgt cccgacaccg ccgctgctcg cgccttgccg gcccgggcgc
      481 ggcggcaatt ggtggagctc ggggccgagt cgatcatccg gatgatctac caggacgggt
      541 tctttcacgc cgacctccac cccgggaacc tcttgattct ccccggtgcc cgcatcggct
      601 tcatcgacct gggcatggtg ggacggctgg acgccgaggt gcggcggctc atgctgtact
      661 actactacag cctggtgacg ggagacgcgg aaaacgccgc gcgctacctc accgcagtgg
      721 cccagcctgg acgccgcggg gatccccagg gcttccgccg ggaggtgacc gagctgtcgc
      781 atcggtggag ccggaccgcc acgttcgagg gattctcgct cgcgcagctc attctcgaat
      841 cggtgtcgcg gggcggaaag taccggatgt atttccccgt ggagctggtg ctcatggtca
      901 aggccctcgt gaccttcgaa ggcgtgggcc acatgctgct tcccggcttc gatgttgcgg
      961 aggtctcccg ccgctacatc cgggagatct tcctggccca gttcagcccg ctccggctgg
     1021 ttcaggagga gctccgcggg gcccccgatc tgatggacgc ggcgatcaag atccccctcc
     1081 tcgtcaccga gggggtccga gtcctggagc gcaacgcccg gcggccggcc gagaatccgc
     1141 tggctggcgt gcggggcacg ttgatcgccg ggttctgtct cgtggccgga gcgatcctga
     1201 tggcgttcgg cggcccctgg ccgctgtggg tcgccctgtt cggagccgcc ttggcgctgg
     1261 cggtcaggcg gggtccctga catggcggcg cctccggtcg gcgcgccggc gacggaccgc
     1321 cactcccgtc ggctccgcct ctggtacgac cccttcgacc cggagtactg ccgcgacctc
     1381 ctcgaccgaa cccttggtcc cgtcgcccgt cagtatttcc gcccccggtt gatcggcgcg
     1441 gagcgcctgc cggcccgggg cccggcgatc ctcgcggcca atcacagcgg caacgcattc
     1501 ccgtacgatg ccatcatgct ggacgcgacc ctctggcgcg cggacgaatt cgcggtggcc
     1561 cggaaggtcc gctcgctttt cgagccggcg cttaccgagt cgtggtggat gcggccgttc
     1621 ggcatcgaca acttttggcg gcgtggtggc ggcgtggaca tgacgttcga caacttcgag
     1681 cggctgatgc agcgcggaga ccgggtgctc tacttccccg agggtgtgcc cggcatcggg
     1741 aaagggttca accggcgcta tcagctccag cctttcaaga ccagcttcgt cgtcatggcg
     1801 gcgcggcatg gggccccggt gtaccccatc tcgatcgtga atggggagtg gatcgttccc
     1861 ttcgggtaca cgttcaggtt cctggacggg atcatgcaga gggtgttccg cgtgccgttc
     1921 ctgccgatcc ccatcggtct gctgggtgtc gtgttccctt tcatgtggta cttcgcattt
     1981 cccgcacacc tggtcttcgt cgtgggccgg cccatcgacg tccgtgcgat gatgtggaga
     2041 gaggggataa ccgacttcga gtccccaccc cgggccgcgg cccgacgcgt ggccgagcgc
     2101 atccgcgtcc tgatgcagca ggagctcgat gccgaagtgg cgcttcatgg ccggcggccg
     2161 ttcgaggtag cgtcactctg gaaggctgtc gtcggcggga ccctttcctt cggccgggtc
     2221 ctgccgccgg cttggacgct caccatggtc cgccaggacc gggaccggca ccggccggca
     2281 ccacggaacc ggctcctggg cctgctgcgt gattgggacc tgctgttctt ctacgtcccg
     2341 ttcggctggc ccctattgtc gctggcccgc cggctgcggc ggccgccgta cgggtaccgg
     2401 ggtctgcatc ccgaagagcg gcgtgatgtg cagggccagt tcctctggcg gctcgtggat
     2461 cgacccctgc cgccccaccg tcaaccctga cggagtgagt cggccaaccg ggccagggtg
     2521 ttccgaagcg ttcgcgtcgc gccggctgct ccgccggcgc cgccgccggt ggccagaggc
     2581 gcgccctcgg tactcatggt ccgcgtcgta tcgtccagca cgtccccccg gagggtgatg
     2641 ccatgccgcg ccagcgcggt ttcgatctct gcgcgccgct gccgcagggt ccggcgggtg
     2701 ctctggtatg ccagctcgca caccgcgccc cgcgcggaga agctgaagat gttggtgaag
     2761 aacatcccgt aatcatcggg gtggggctcg aagagcacga cgtcagagtc cgggtaccgc
     2821 gtcccataag cggacatgcc gacttccagt cgcgagtgga tcatggtacg caccgtctga
     2881 ctcaataccg tgaccagtcc tccggcggtg agggcgtccg gccgaagcgc gccggtggcg
     2941 atgccggtgg tgatgtccgc cgggaccaga ggattcacgc agagcacgag cccggccccg
     3001 tcctcgagcg ccaccgatgc atggagcgtc ttgagcagca caccgtccac acagggccgg
     3061 ccgtcgatgg tgaccggcgg atagagaccc ggaaacgccg aactcgcctg aacggcccgc
     3121 gagatcggga cgtgatcgaa acccggctcg ccgaaccgga tggcccgtcc cgatcccagg
     3181 tccgtggcca caatcaccag tttcctcttc aactggcgaa agtcgtcggt gcggcccggt
     3241 tgggagagca tccgggcgag gtaccgtcgg atgggctcgt tttcgaagat ggccaccggg
     3301 agtccacctg cgagccggct cagggactcc cgcacggaag ggtctcccgg gtgcacggcg
     3361 acccggctca aggcggtcgc gaacagcttc gggagccggg cgccctgccg cgcccagccc
     3421 cggagggcgg gtttgaagaa cgtcccggga gagaacggcg attccgcccc ctccgagcgg
     3481 acgacggttc gggcgagctc cgacggcttg acaccgttgg cgagggcggc ggtgaggaac
     3541 gcacccgcgg agacgcccac ataaatggag agatcggcga agtccaagcc ctcgaggcat
     3601 tcctcgagcg catagagcgc gccgatctca tagacggcac ccgctggtcc gccgccagcg
     3661 agtgccagcc cgatgtgccg gcgaggcgta cgccgtgcgc cggccatggg atcaactcac
     3721 actgccgcgg ccgctacgac gtgcttcgcg ggcgcccgct tgcggggtct tgccttggcc
     3781 cgctccacgc tccgagtcaa ctcttccacc cgcttggtca gggtcacgat ctcctgacgg
     3841 gacgggatcc cgaagcggtg cagggcacca gccatcccgg tgtcgacggg aagacggaag
     3901 cgcccaacgc tccggcgggc attctcgcgg atcttgttca cgcgggcgag ggccttcccg
     3961 accttctggc ggttggcctt gtccaccgta tggcccttct tgaccaggga gccgaacagt
     4021 ttcccgccct cctcttcggc caacgccaaa gcgcccaggc cggccagcca gacctgctgc
     4081 gccgactgcc tgacgagggg catcctcttg tgttgaatca ccttcgccat atcccacctc
     4141 cgttcgagtt cgcgcctgaa atcaccattg aatcgtaggt gggcggcgtg aagctacttt
     4201 gaatccgggg tgaagccccc ttatcttccc ccctcttttc ctggcaccac cccgaatggg
     4261 agtcccgatc aaatgaaggc cacagacatc cgccgcgggc acgtgatcat gatggatggc
     4321 aatccgtgcc gcgtggaaga attcacacac cgcacccccg gaaatctccg cgcgttcgtg
     4381 cagctccgtc tgcgcaacct gaactccggt aacacgttcg acacacgcgt ctccgccacc
     4441 gacttcctgg atgaagtccg tctggatcag aaggacttcc aggtgctcta tcgcgatgag
     4501 agcggcgtgc acgtcatgga cgccgagaac tacgagcagt acacgctgga caagggcgca
     4561 gtgggtgatc agggcgattg gttgcagcct gaaatgcgct tccaggccga gtggctggac
     4621 ggcaagccca tcggcatcaa gctgcccgcg gtgatggagt tggacgtggt ggagacctct
     4681 ccggtgatga agtccgccac caagacggcc tcgagcaagc cggccaagct gtccaacggg
     4741 gtcaccatcc aggtgcctga gttcatcgct accggcgacc gcctccgggt ggacccccgg
     4801 gaggggaagt acctcgaaag ggccaagtag ctctgacgta gtccgaatcg acaacgcgcg
     4861 gcgcccccct tccgtaacga ggggggcgcc gcagtttttg ggggcctttc cacccttcga
     4921 gatcaggaga cgtcaatgca ccgcccgggt gtccttccct ttctaacgct cgccggactc
     4981 gtcagcctgg cgactcccct tgccgcgcag gccaagccca accgcaagcc ggcggcgaaa
     5041 gctccggcgg acgccaccgc gacggcatcc gctgctccgg cggcctcacc ggaagactcg
     5101 ctcaatacga ttcgtttccg gaaccttggt ccttcggtgg ccggggggcg cgtgtcgacg
     5161 gtggtgggca tccccggaca gccggggtct tactatgtgg gcgccggcgc cggtggcgtg
     5221 tggaagacca cggataacgg caacacctgg gaagcgatct tcaaggatca gccggcggcg
     5281 tcgatcggtg cggtggcgct cgcgccgtcg aacccgaatc tggtctgggt ggggacgggc
     5341 gaggcggcca tccgcaacga cgtggtgaac ggacatggcg tctggttctc gcccgatgcc
     5401 ggtcacagct ggcgcttcat gggactcggc gacgtgggtc agatttcgaa gatcgtggtc
     5461 gacccagcca acccggacgt cgtattggtg gcggcgctgg gtcatgcctg gagccccaat
     5521 ggcgatcgcg gaatcttccg caccacggac ggcgggaaga cctggcagaa gactctcttc
     5581 gtcaacgaca ccaccggtgc ctcggacttg atcatggatc cgggcaaccc gcgcgtctat
     5641 ttcgcgggga tgtggcaatt cgtccgtcac ccctgggagc tgacctcggg gggcgcgggg
     5701 tcgggcatct accgctcgac cgatggtggc gtgacgtggg agcgcttgtc gcaaggcttg
     5761 ccccggggta tctacgggcg catcgcgctc ggcatcggcg cgtccaaccc gagccacatc
     5821 tacgcgctgg tcgaagcgaa gaccggcatg ctctgggagt cgcatgacct cggcgatcac
     5881 tggtcgagcg tgtcggacaa ccatgccctg gatgtgcgtc ctttctattt ctcccaggtc
     5941 acggtctctc ccatcgacga tcggaagttg tacttctcct cctacgagct gctcgagtcc
     6001 gctgacggcg ggaagacggc caaggccatc gatcagggcg tccatgtcga tcaccacgcg
     6061 ctctggatcg atccgacgaa tgcgcagcgc atcatccagg gcaatgacgg tggcgcgtac
     6121 gcgaccacgg acggcgggaa gaactggacc tggttcaaca acctgcccat cgagcagttc
     6181 tacatggtgg ccgcgaccag tggcgacccc tacatgatct gcggtggact gcaggacaac
     6241 aacggctggt gcgggcccgt gggggcgagt gacggcgcgc aatggttcac ggtcaccgga
     6301 ggcgacgggg agtacgtcgt tccggcaccc tccgattcga ccatcctgta cgtggattcc
     6361 cagaatgggt ccatcagccg cgtgaacctc cggaccgggg agtcccatgg gatccggccg
     6421 tacgagccga gcgtcgaagg gacgaagcct tcggagctga cttaccgctt caactggacc
     6481 tcccccattg aagtctcggc cacgaacgcc gacgaggtgt tcatcggcgg caacgtggta
     6541 ttcaagagca ccgatggtgg ccagcactgg gtgccgatca gtcccgatct tacgcggaat
     6601 gacaagacga agcaggtgaa ttccggcggt cccatcgagt acgacatctc aggcgccgag
     6661 acgtacaaca cgattctgac cgtgaatgtg gcgccgaccg atacgaacgt gatctgggtg
     6721 gggaccgacg acggcctggt gcaggtgacg aaggatggcg ggaagacctg gaccaacgtc
     6781 ggcggccatt tccccggtct cgcggcggat gaggaaggac gggtctacca ggtcgggatt
     6841 tcgcccttcg acgccggcac ggcctacgtc acctacgacc ggcacgagtt cgacgacaat
     6901 cgggcctatg cctacaagac cgccgactac ggcaagtcgt ggacgcgcat cgatgccggc
     6961 cttccggagt acgcggcgca tgtggtccgc gaagatccga acgcccggaa cttcctggtg
     7021 ctgggcaccg accagggact ctggttctcc cggaacggtg gcgcctcgtg gaaggccatg
     7081 aaggcggatt tcccgacgac gccggtgtac gacgtgaagt tcgtcaaagc ctcgcacgac
     7141 ctgctggtcg cgacccatgg ccgcgggctt tttgcgctcg acaacatcaa ttccctcgaa
     7201 ggcctgaccg ccgaggtggc ggcgcaggac ttccatctct tcccggtggt ccccgcggtt
     7261 ctgcggggac gcgcgcgggg ccgttccggt gtggcgccga cgaatttcac ggtccgcggc
     7321 gcgccgcagg gcgcggtgat cgattactac ctgaaggcag gggtggacac gacgtccccc
     7381 gcgggcagtc ctgaagcggg tggccctggt ggcggtcgcg gtgggcgcgg cggccccggt
     7441 ggattcggtg ggggccggcg gtcgggcgcg cggctggtgg tcacgagcgc ggccggtgat
     7501 acggtcttcg tcgattcgtc gggaccgacc aagatgggcg tgaaccggat gacgtggggc
     7561 ctgcggtgga atggtccgac ccccctcacc atcgagcccc cgcagggtgg tgaagggggc
     7621 ggcgggttcc gtcgcggtgg cttcggcccg gcggtcgtgc ccgggacgta cacggtgacg
     7681 gtgtcggtgg cgggcaagag cggctcgcag acggtggaag tgaagcccga tccggttcaa
     7741 ggaggcgacc cggccaagtt ccaggcgcag ctggcggccg gtctgcaatt ccggtcggag
     7801 ctgacggcac tgcacgagat gctgaaccac ctggtggcca tcgagacgca gatccggaac
     7861 gcgcagaccg ccgtgcgtga gacgggtgac acggcggcga tgggtgcatt gaaccgggcg
     7921 tcgcgcgatc tgtcgcggaa gctccgtcag ctgaaggaca cgctctacaa ctcggatcag
     7981 cagcggggcg cgccggagga cgacattcac tatctcacgc gtttcgccga caagtaccag
     8041 agtcttggat tcggggtctt cggcggatac gcgcaaccgc cttcaccgca gttgatggat
     8101 gagtggaagg tagaacgggc gcaactggat gggttcatcg cgacgttcaa cgggattctg
     8161 aacacggatg tcgcggcgta caatcaggta gccaacgcga atcacacgcc ggtgctgctc
     8221 ggcgggggtc cgatcgcgat caaggcggcg tcagcccgct gattcgagaa ccgtcgcgac
     8281 ctgtttgagc agggtgtcct tgtcgatcgg cttgtgcagc accgcccgcg ctccgcgggc
     8341 ggtgctgtcc gcgtcgacct tcgcgtcatg gacggcggtc acgaccacca cggggatctc
     8401 tcgggtcttt gcggacttgt tgagccggtc gaagacggtc atgcccccgc ccgcgggcat
     8461 gttgatgtcc agcagaatca cgtcggggag ctcgcgctgg gcgaacatga atccctgcat
     8521 ggcgtcttcg gcgatcatcg gcacgtatcc cgctccccgc aagtgagccg cgaacagctc
     8581 gcacagcggg cggtcgtcgt cgatgatcag cactctcgct tttttcgtct tggccatggt
     8641 ggtcccgcta gacctcgccg tgtcggaaac aggtcaccag gtggtcgttg accattccca
     8701 ccgcctgcat gaacgcgtaa cagatggttg ggcccacgaa cttgaagccc cgctcagaca
     8761 aaatgtcact catccggcgg gattcgcgag tctcggcggg gaggcctcgt tgcgccctgc
     8821 gggcgttctt gatgggggcc ccccccacgc agtcccaaag cagc
//
LOCUS       DASUBP010000199        17055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_264564, whole genome shotgun sequence.
ACCESSION   DASUBP010000199 DASUBP010000000
VERSION     DASUBP010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 17055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17055
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_264564"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1519
                     /locus_tag="VFU75_07500"
     CDS             <1..1519
                     /locus_tag="VFU75_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HEU5466720.1"
                     /translation="REVLRVLRELTDLLRPHRDLLGRSLEMCVAFDDLCARARYAVVV
                     NGHVPVIGDAALAIHGGRHPLLLGGDIAVVPFDLVLRSDEWTVLVSGPNTGGKTVLIK
                     AVGLIALLAQSGIIPPVGPRSTLPVFTGVFADIGDKQSIAESLSTFSAHTRTLREILD
                     GAGAGSLVLLDEIGSGTDPAEGGALASATLRALTRRHAVTLATTHLGALKQLAAETVG
                     IVNASLQFDAETLTPSYRLLKGVPGRSYGLAIARRLGVADDVLADAERSVPDTERQLD
                     RLLAEVESRRQELERHARELEERATALGEEREGVERRGEDVAERERAIRDRERSLDTA
                     ARDQARAYLLDARKTVEAALAQARAAVDEATAKEARRLVEEAIEKSESDRETGNERRE
                     TSRGWTSLDDLKRARGADTFPVSRVSFPETSTGATEISLIGMRAGEAEGVLLQAIDAA
                     VSEDLPYLRIIHGKGTGALRKMVHDVLGNDRRVRRFAFAPANQGGAGATVAEFKA"
     gene            1516..3282
                     /gene="dnaG"
                     /locus_tag="VFU75_07505"
     CDS             1516..3282
                     /gene="dnaG"
                     /locus_tag="VFU75_07505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEU5466721.1"
                     /translation="MSLIPDELIEQVRDAADLVGIIGEHVELKRTGTDYRGPCPFHGG
                     THRNFAVIPKKGMYYCFVCHEAGDVFTFFMKKLGMDYPTAVREVARRVGITIPERGPT
                     GPDPREPLFQAVSAAHDWFQRQLRESPEGKIARDYLASRHIDLETAGVLGLGFGPRGK
                     AFAEAMKQLGVKEELLLETGMLVKRDDGSIIPRFRERVLFPILDLRGRVVAFGGRVLG
                     DGEPKYLNSPETPIFHKGQMLYNLHTARHAMRKAERAVLVEGYFDTIRLSLAGIEEVV
                     APLGTGLTSDQAELLKRHTEHVILLYDSDDAGLRATFRAGDELLHHGLRVSVATLPEG
                     EDPDTLVQAQGGPALERVLKDAVDVFDRKLQILERRGMLTTIPGRRRALDRLLPTIRA
                     TRDPITRELYLARAAEAAHVRKDVLEREVAIAGVGKREAGSGAPATLPSSRVPLPVSP
                     SAERALLLALLAGEPWRSRVLESVDTEDFDVPANREVFEAERAGSAAELGGDAARIYE
                     QLRAEKVEEQGVGDMLEGAMRHVNARPLSAKIESIDRAMAVASPEEQRRLMVKKQQLT
                     AERNAVKPTYKIVSRRKGAPGS"
     gene            3266..3970
                     /locus_tag="VFU75_07510"
     CDS             3266..3970
                     /locus_tag="VFU75_07510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466722.1"
                     /translation="MHPDLEALLMLQQKDEAVRDTDARLAALEPELARLDQVLEGVEQ
                     KLELARRGIEDAVGRRGELEGKIASYRGMQEARKQRLEWVKGAKEAQTLMAELDLART
                     VIAKEEIEFLRSGDAVTEAEMKAAEAEKGVEEVREAQRADREIIDGKRQAIIADREKA
                     IVERERAAKFVQASLLSRYEKIRKGKRPVAVYPLHGGSCGHCFTAVPTQRRVLIQRGV
                     TIEGCEACGVLLYVAG"
     gene            3967..5673
                     /gene="recJ"
                     /locus_tag="VFU75_07515"
     CDS             3967..5673
                     /gene="recJ"
                     /locus_tag="VFU75_07515"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEU5466723.1"
                     /translation="MIPARWALTAAPSAAAIHDLSAALTIPEPLAALLIQRGLGQPDL
                     AKQFLRPELERLSNPLEWTGMKRAVEIVSQAVRDHVPILVHGDYDVDGQCASSMLTRA
                     LRAAGGNVHAFVPHRIRDGYDFGPAGLAKAASIGARLIITCDCGITAVETVARARAAG
                     HQVIVTDHHLPGDALPEADAVLDPQQPGCPSVDKTLCGAGVAFKLVQALVPALGLSPN
                     LPLHFLDFVALATVADVVPLTGENRILVRHGLKMLSDSRWTGLRALVEAAGLAGQPIR
                     AGQVGFILAPRLNAAGRIGDANDGLKLLLTDDPAEAAALAREMETLNARRQEMDHRIQ
                     DEAIEMAEKSLTPDQCGLVLASDGWHPGVIGIVASRLVERYGRPTFLIGWDGDLGKGS
                     GRSVLGFDLHGALHEVGACLEKYGGHTMAAGLTIQRPRFEEFREGFLAAAARILTPDD
                     LAPAQRVDLELPLGSVSDDLERLMRHLEPCGAGNPAPVFGVRGARAVDARRVGANHLK
                     FKLDDGSGVLDAIGFRWADQVPDAWLASPLDVAFRLERDEWQGRFRLQAKVASIAPSA
                     AA"
     gene            5964..6479
                     /gene="rsmD"
                     /locus_tag="VFU75_07520"
     CDS             5964..6479
                     /gene="rsmD"
                     /locus_tag="VFU75_07520"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: protein motif:HMM:TIGR00095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEU5466724.1"
                     /translation="MIEIVAGEFRRRRLKTPAGDKTRPTSGRVREAWFSILQTSIPGA
                     RVLDLFAGSGALGLEALSRGATSADFVERARPALDALRANIETLKVQDRVTVHRADAL
                     KFVERLQPGQYDVAFADPPYADPAAERLSALFRTAPFARIFAVEHSADTPVQGDDSRR
                     YGDTAITFFYA"
     gene            6482..6979
                     /gene="coaD"
                     /locus_tag="VFU75_07525"
     CDS             6482..6979
                     /gene="coaD"
                     /locus_tag="VFU75_07525"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEU5466725.1"
                     /translation="MTRIAVYPGSFDPVTRGHADLIRRSLAFSDQVVVAVAVNVSKQP
                     LFTLEERVALIRETVKVPNVEVRSFDGLLVDFAKQVGASILIRGLRAVSDFEYEFQMA
                     LMNRTLAPALETVFLVPAFDLTYLSSSLVREVARFGGDVSALVYPAVQQALKAKFKSL
                     SGSKA"
     gene            6976..7311
                     /locus_tag="VFU75_07530"
     CDS             6976..7311
                     /locus_tag="VFU75_07530"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HEU5466726.1"
                     /translation="MTFSIKKDAKGVVVVGVDGQLIVGNRQELKQKVLESLEGGARKF
                     VVDFSTTGYIDSSGLGVLVSLSKKIREQGGDLRLAGLNEDLQTLFELTKLDTLFTIAK
                     TPAEALAAF"
     gene            7313..7753
                     /locus_tag="VFU75_07535"
     CDS             7313..7753
                     /locus_tag="VFU75_07535"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5466727.1"
                     /translation="MKIAVRRAGYSGDVELLLRVPSDLSFVAEAVDLVASHTPSGILS
                     PRRVQFNLRTVLAEALANAITYGNREDPTKKVEVRVEARSEFIRVTVTDSGEGFDPSV
                     VPDPTAPEGVEREFGRGLFVIRHLVDAVDFNAKGNAVCLTLRAG"
     gene            7810..9252
                     /locus_tag="VFU75_07540"
     CDS             7810..9252
                     /locus_tag="VFU75_07540"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing SpoIIE family protein
                     phosphatase"
                     /protein_id="HEU5466728.1"
                     /translation="MRTGKAAAGRGWKGTAVPGFTDTWLEVKRAGRGRVNARALAKTV
                     AALLEAEEETKAVAAELSARYEEIDLIYTISEVLGHTIRLSDAASRILREVSDVVGAR
                     RATLLAVDEGGKVLRIVAAQGMNPSEAEPIEVDDPSSIAARVFREGRILSYNPPVGAA
                     STAANRESRSYRGYAYLSVPVLYATPGGPSRPIGVINFTDRIGGDAFTTGDRKLVAAI
                     ANQIGAALANARLVSRDLEQQRLRRELELARDLQLKLLPSPLVIAAKADVAARCRPAE
                     VVGGDFYNLMRLPGDRIGVMIGDVSSHGFGAALIMALAMSASGIHAESATSPAAVMTL
                     LEQSLADELQKTEMFLTLFYGVIDPAAGKLFYANAGHPQAFVVRSDGTSCRLEATRPP
                     LGLGVGGTADAEQPWAKRADTLCLFTDGISDAMSLRGVRFGEERVLGHVSRMRGRPTL
                     DILNAVTTELDAFTEGADPTDDRTIVLLRV"
     gene            9249..10088
                     /gene="rsmA"
                     /locus_tag="VFU75_07545"
     CDS             9249..10088
                     /gene="rsmA"
                     /locus_tag="VFU75_07545"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:NF012616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEU5466729.1"
                     /translation="MKPKKSLGQHFLSDVGILGRIADALDLSPGETVLEIGPGRGDLT
                     RQLLARGARVVAVEKDERLAAALTGNVTRETGNVRVVEGDALEIDWHPLVRDTFPVSR
                     FPFPDSRFPFPGFSVIGNIPYYITSPLLEKALTPPLPSRIVFLVQKEVADRIVAPPGS
                     RTYGALSVGIQAVCTVEKLFVVKAGAFRPPPKVDSAVIRLTPLEQPLVPVEQGTAFRR
                     FVVAAFGQRRKQLRNVLARVTGRPAAAVADEIRALGFDPQSRPETLSPADFARLLIWS
                     RQL"
     gene            10100..10720
                     /locus_tag="VFU75_07550"
     CDS             10100..10720
                     /locus_tag="VFU75_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA];
                     GO_process: GO:0051775 - response to redox state [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEU5466730.1"
                     /translation="MRKISESTVRRLSLYLQYLELTQDQGRTTVSSEELARRSGATSA
                     QVRKDLSFFGSFGKRGMGYEVPALITRLREILGLERAYRVVLVGAGKIGGALIHYEGF
                     RRRGFEVSAIYDRDPKKIGSRSNGLVVRDVKQLEADLKKEPTDIGIVVTPGDAAQEVA
                     DRLVRAGVKALLNFAPVQLKVPRDVEVKTVNMAQELEALSYSLANR"
     gene            10767..11999
                     /locus_tag="VFU75_07555"
     CDS             10767..11999
                     /locus_tag="VFU75_07555"
                     /inference="COORDINATES: protein motif:HMM:NF014344.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HEU5466731.1"
                     /translation="MPSVRVTSEIGPLKAVLVHTPGPELVAVTPGNREDYLYDDIIDL
                     DLAQREHRRFVAVLERFAEVYEVRDLVTEVVAQKDARDFLVTRTLDVVPSDALAQRMA
                     ELPAEALVGLMVEGALEEAGPIARALNEGGYALPPLPNLFFTRDTGMVIGNHCVIGSM
                     RYGVRWTEELLIKSLFKYHPKLENAGILYDGSEEKRSNYTLEGGDVHPIRPDLMVLGF
                     SERSTPAALDQLCDLAFEQCGVTDVVIVVLPAERTAIHLDMIFTQIDREACCIYPPHF
                     IGPERLAVLHRRKKSKGVKEMPNFFAAMQAVNQPLEPVFCGGPSRSLQEREQWSSACN
                     FFTVRPGVAVTYARNEATLREFEHAGFRIISAVNLLTGDEYLEDGERGIITIEGSELV
                     RGGGGPRCMTLPLRRDDL"
     gene            11996..12862
                     /locus_tag="VFU75_07560"
     CDS             11996..12862
                     /locus_tag="VFU75_07560"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5466732.1"
                     /translation="MTLHTTFQIGHLKAHAIESGYLRLDGGSMFGVIPKTLWEKHIPA
                     DQRNRIRLAMRCLLIEHPDGLVLIDTGLGNKEDAKFLDIYGVDNGGVDRRTRLEDGIR
                     EAGHAPSDVRWVINTHLHFDHAGGNTWKDHMGEINKTFSKATYVVQRREYEFATHTNE
                     RTRGSYFPLNWEGIDFTLLNGEQEVLPGIKGIPTPGHVPGHQSILVESGGEKLLFIAD
                     LVPTASHLPLVWIMGYDVEPMVTLETRRHLYPRAAAEGWRLFFEHDATTIEGRLEKDG
                     KGFALVAPVMLG"
     gene            complement(13083..13214)
                     /locus_tag="VFU75_07565"
     CDS             complement(13083..13214)
                     /locus_tag="VFU75_07565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466733.1"
                     /translation="MSAITRTGLIRIWRFTRVRGVRLLLVLLGLPVLLVLLLLFPGS"
     gene            13195..13656
                     /gene="infC"
                     /locus_tag="VFU75_07570"
     CDS             13195..13656
                     /gene="infC"
                     /locus_tag="VFU75_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEU5466734.1"
                     /translation="MRVIADNGDQLGVLSIDEALNAAAERGLDLVEVAPMARPPVVKI
                     MDYGKYKFEQAKAARAAKKKQHVIHLKEVKFRPGIDDHDFQFKTRHAREFLEEGNKVK
                     VTMMYRGRQMAHIDLGRQVLDRVAVELKDISKIEQEPKLEGRNMSMVLGPK"
     gene            13744..13938
                     /gene="rpmI"
                     /locus_tag="VFU75_07575"
     CDS             13744..13938
                     /gene="rpmI"
                     /locus_tag="VFU75_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEU5466735.1"
                     /translation="MPKMKSKRALRKRVKITGSGKIRRHRAFKSHILTKKHPKRKRRL
                     RQGAMVSHADQKRLMRLLQA"
     gene            13948..14301
                     /gene="rplT"
                     /locus_tag="VFU75_07580"
     CDS             13948..14301
                     /gene="rplT"
                     /locus_tag="VFU75_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEU5466736.1"
                     /translation="MPRVKSNVARLARKNKIMKLAKGYWGGRSKLWKAAKESVERAEK
                     YAYRDRRRKKGDFRRLWITRINAAARLHELSYNRLMNGLRKAGVEINRKVLADIAVRD
                     PVAFEQLAEVAKKAL"
     gene            14303..15334
                     /gene="pheS"
                     /locus_tag="VFU75_07585"
     CDS             14303..15334
                     /gene="pheS"
                     /locus_tag="VFU75_07585"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002639667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEU5466737.1"
                     /translation="MRDLLDSLARLRERLAEIREVDASRLQELKTELLGRKAGALTEI
                     LKQLPTLDPESRKAVGGQANALKAQLEDAIATRERALATAARKPTGVDLTMPGRPAWR
                     GGLHLTTQVVNEICGIFAELGFTRAVGPEAETERNNFFALNFPPDHPALDSQDSFYLG
                     KDTLLRTHTSPVQVRTLEAYPPPIRVVIPGRVYRRDPFDASHSPVFEQIEGLVVDEGV
                     TFVDLKAALTAFARRFFSPSTKVRFRPSFFPFTEPSAEMDVQHPLCGGAGCPTCKGTG
                     WMEILGSGMVHPAVLEKCGVDAERYTGYAFGMGPERIAMSRHGIPDIRLLFGDDVRFL
                     SQFSEGGKR"
     gene            15331..>17055
                     /gene="pheT"
                     /locus_tag="VFU75_07590"
     CDS             15331..>17055
                     /gene="pheT"
                     /locus_tag="VFU75_07590"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEU5466738.1"
                     /translation="MNVSRRWLEALLDQPLDAKETADRLSNLCAPVDGIVPLHHDLGD
                     VLIAHVLEVQKHPNADRLSLCKVDAGGPEGPVEVVCGAPNVTAGKTYPYAPVGATLPG
                     GLTLERRKIRGVTSNGMLCSAKELGLGQDHDGILELATTAKPGSRFLDALPIADEQII
                     IDVGANRPDLLCHKGVARELAVAFGGRIKLPAIPGSTGAGAAMPAPRRATTEGKVGGV
                     TITLQDTGGCRRYLAAVIRGVKVAKSPAWLSDRLEAVGQRSVNNVVDATNYVLFELNQ
                     PMHAFDLAKLRGPAVVVRRANAGERIVTLDGVERTMTAEMTAICDAERPTIVAGVMGS
                     AESEVTDQTTDLVLECAYFDPTRTRRTRRALGLSSESSYRYERGIDLWGMPDALRRAI
                     DVITAVAGGTLADAPLDLWPEPEQPRAVFLRPERVNHLLGIEVPRTEIEKLLTSVGFV
                     ALPKDDRMAVQVPGWRPDVTREVDLIEEVARLRGYDSFPDELRAFRPGTVPDSPVEQA
                     KDRVREVLVGAGFYEARTLSLGPANGPGAVAVSNPLSAEDAHLRMALLPALRRRVEGN
                     WAEKNRDVR"
BASE COUNT         2861 a         5625 c         5876 g         2693 t
ORIGIN      
        1 gcgcgaggtg ttgcgcgtgc tccgggagct gaccgatctc ctccggccgc accgggatct
       61 gctcgggcgg tcgctcgaga tgtgcgtggc gttcgatgac ctgtgtgccc gggcgcggta
      121 cgcggtggtc gtgaatggcc atgtcccggt gatcggtgac gccgcgcttg ccatccacgg
      181 cgggcgccat cccttgctcc tgggcggcga catcgccgtc gtgccgttcg atctcgtact
      241 ccggagcgac gagtggacgg tgctggtgtc gggccccaat acgggcggca agacggtact
      301 gatcaaggcc gtgggcttga tcgctctgct cgcgcagagc ggcatcatcc cgccggtcgg
      361 cccgcgctcg acgctcccgg tgttcaccgg cgtcttcgcc gatatcggcg acaagcagtc
      421 catcgccgag agcctttcga cgttttccgc gcacacccgc accctccggg aaatcctgga
      481 tggtgccggc gcgggttcgc tcgtactgct cgacgaaatc gggagcggta ccgaccccgc
      541 cgagggcggg gccctggcct ccgccacgct ccgcgcgctc actcggcgcc atgcggtgac
      601 gctcgcgacc acccacctgg gtgcgctcaa gcagctcgcg gcggagacgg tggggatcgt
      661 caacgcgtcc ttgcagttcg acgccgagac gctcacgcca tcctatcggc tgctgaaagg
      721 cgtgccggga cggtcgtacg ggctggcgat cgcccggcgg cttggcgtcg cggacgacgt
      781 gctcgccgat gcggagcgat cggttcctga caccgaacgc cagctggatc ggctcctcgc
      841 ggaggtcgaa agccggcgtc aggaactcga gcggcacgcg cgggagctgg aggagcgggc
      901 gacagctctc ggagaagagc gcgagggcgt ggagcgccgg ggagaggatg tcgccgaacg
      961 ggagcgcgcg atccgggacc gggaacgctc cctcgatacg gccgcccgcg accaggcccg
     1021 cgcgtacctc ctcgatgccc ggaaaaccgt cgaagcggcg ctggcgcagg cgagggccgc
     1081 ggtggacgaa gcgacggcga aagaagcgcg gagactcgtc gaggaagcga tcgagaagag
     1141 tgagagcgat cgggaaacgg gaaacgagag acgggaaacg tcccggggat ggacgtcact
     1201 cgacgatctc aagcgagctc gcggtgccga cacgtttccc gtttcccgtg tctcgtttcc
     1261 cgaaacgtcg actggcgcca ctgagatctc gttgattgga atgcgcgctg gggaagcaga
     1321 aggtgtgctg cttcaggcca tcgatgccgc cgtatcggag gacctcccat acctccggat
     1381 catccacggc aaaggcacgg gcgcgctgag gaagatggtg cacgacgtcc tgggcaacga
     1441 ccggcgcgtg cggcggttcg cgttcgcgcc cgccaatcag ggtggcgcgg gagccacggt
     1501 ggcggagttc aaggcgtgag cctcatcccc gacgagctga tcgagcaagt ccgcgacgcg
     1561 gcggatctgg tcggcatcat cggcgagcac gtcgagctca agcgcaccgg caccgactac
     1621 cggggaccct gtcccttcca cggcggcacg catcgcaact tcgcggtcat ccccaagaag
     1681 gggatgtact actgcttcgt ctgtcatgag gcgggggatg tcttcacctt cttcatgaag
     1741 aagctgggca tggactatcc caccgcggtt cgcgaggtcg cccgccgcgt gggcatcacc
     1801 atccccgaac gcggacccac cggcccggac ccgagggagc ccctcttcca ggccgtctcc
     1861 gcggcgcacg actggttcca gcggcagctg cgggagtctc cggaagggaa aatcgcgcgc
     1921 gactacctcg cctcccgcca tatcgacctc gaaaccgccg gcgtcctcgg cctggggttc
     1981 ggcccgcggg ggaaggcgtt cgctgaggcc atgaagcagc tcggcgtgaa ggaagagctg
     2041 ctgctcgaga cgggaatgct ggtgaagcgc gatgacggct cgatcatccc gcgtttccgc
     2101 gaacgcgtgc tctttcccat cctcgatctc cgcggccggg tcgtcgcctt cggcggccgc
     2161 gtgctcggtg acggcgagcc gaagtacttg aactcgcccg agacgccgat ttttcacaag
     2221 gggcagatgc tctacaacct gcacacggcc cggcatgcga tgcgcaaagc cgaacgggcc
     2281 gtgctggtcg aagggtattt cgacaccatc cgcctttccc tcgcagggat cgaagaagtc
     2341 gtcgcgcccc tcggcaccgg gctcacgagc gatcaggccg agctgctcaa gcgccacacg
     2401 gagcacgtca ttctcctgta tgactcggac gacgcggggc tccgcgcgac gttccgggcg
     2461 ggggatgagc tgctgcatca cggattgcgc gtgagtgtgg cgaccctccc cgaaggggaa
     2521 gatcccgaca cgctcgtgca ggcgcagggc ggccccgccc tcgagcgcgt gctcaaggac
     2581 gccgtggacg tcttcgaccg gaagctccag atcctcgagc gccgcgggat gctcacgacg
     2641 attccgggcc gccgtcgcgc cctggatcgc ctgctgccga ccatccgcgc cacccgcgat
     2701 cccatcaccc gggagctgta cctggctcgc gcggcggaag ccgctcacgt gcggaaggac
     2761 gtcttggagc gggaagtggc gattgcggga gtggggaaga gggaagcggg aagtggtgcg
     2821 cctgccacgc ttccctcttc ccgcgtccct cttcccgttt ccccttccgc cgagcgtgcc
     2881 ttacttctcg cgctcctcgc cggcgagcct tggcgcagcc gggtgctgga atcggtcgac
     2941 accgaggact tcgacgtccc ggccaaccgg gaagtgttcg aagccgagcg cgcggggagc
     3001 gccgcggaac tggggggcga cgccgcacgg atctacgaac agttgagggc ggagaaggtc
     3061 gaagagcagg gcgtcggtga catgctggaa ggggccatgc ggcacgtgaa tgcccgcccc
     3121 ctttccgcca agatcgaatc gatcgaccgc gccatggcgg tcgcctcacc ggaagagcag
     3181 cgccggttga tggtgaagaa gcagcagctc accgccgaac ggaacgccgt caaaccaacc
     3241 tacaaaattg tgtcgaggag gaagggtgca cccggatctt gaagcgctgt tgatgctcca
     3301 gcagaaggat gaagcggttc gcgacacgga tgcccggctg gcggccctgg agccggagct
     3361 ggccaggctc gaccaggtgc tggaaggggt cgagcaaaaa ctggagctgg ctcggcgggg
     3421 aatcgaggac gcggtgggac gacgcgggga gctggaaggc aagatcgcga gctaccgcgg
     3481 catgcaggaa gcccggaagc agcgcctcga atgggtcaaa ggcgccaaag aagcccagac
     3541 cctgatggcc gagctcgatc tggcgcgcac cgtgatcgcc aaggaagaaa tcgaattcct
     3601 ccggtcgggt gacgcggtga cggaggcgga aatgaaggcc gccgaagcgg agaagggcgt
     3661 cgaagaggtc cgggaggccc agcgggccga tcgcgagatc atcgacggca agcggcaggc
     3721 catcatcgcg gaccgggaga aggcgatcgt cgagcgggaa cgagcggcca agttcgtgca
     3781 ggcctcactc ctttcacgat acgagaagat ccggaagggg aaacggccgg tggcggtgta
     3841 tccgctccac ggtggctcct gcggccactg cttcaccgcg gtaccgactc agcgaagagt
     3901 gctgatccag cgcggggtga ccatcgaagg ttgcgaggcg tgcggcgtgc tgctttacgt
     3961 agcgggatga ttccagccag gtgggccctc accgccgcgc cgagtgccgc cgcgatccat
     4021 gatctgtcgg cggcgctgac cattcccgag cccctcgcgg cgctcctgat ccagcgcggc
     4081 ctcggccagc ccgatctcgc gaagcaattc ctgcggcccg agctcgagcg tctgtccaac
     4141 ccgctcgagt ggacgggcat gaagcgggcg gtcgagatcg tgtcgcaagc cgtccgtgat
     4201 cacgtgccga tcctggtcca tggcgactac gacgtagacg gacagtgtgc gtcgtccatg
     4261 ttgacgagag ccttgcgggc ggccggtggc aacgtgcacg cgttcgtccc gcaccggatc
     4321 cgcgacgggt acgacttcgg gcccgcgggg ctcgccaagg cggcgagcat cggtgcccgg
     4381 ctgatcatca cctgcgactg cggcattacg gccgtcgaga ccgtcgccag ggcgcgggct
     4441 gccgggcatc aggtgatcgt gaccgaccac cacctccccg gcgacgcact ccccgaggcc
     4501 gacgcggtgc tcgatccgca gcagcccggc tgcccctcgg tcgacaagac cctgtgcggc
     4561 gccggcgtcg ccttcaagct cgtccaggcc ctggtccccg cgctcggtct ctcgccgaat
     4621 ctgccgctcc attttctgga cttcgtcgcc ctggcgacgg tggccgacgt cgtgcccctc
     4681 accggcgaaa accggatcct ggtgcgccac ggactcaaga tgctctcgga ttcgcgctgg
     4741 accgggttgc gggcactcgt cgaagcggcg ggtctcgcgg gtcagccgat ccgcgcggga
     4801 caggtcgggt tcatcctcgc gccgcggctc aatgcggcgg gccgcatcgg cgacgcgaac
     4861 gacggcctca agctgctcct caccgacgac ccggccgaag cggcggcgct cgcgcgggag
     4921 atggagacgc tgaacgcccg gcggcaggaa atggatcacc ggatccagga cgaagcgatc
     4981 gagatggcgg agaagtcgct gacgcccgat cagtgcgggc tggtgctggc gagcgacggc
     5041 tggcacccgg gcgtgatcgg catcgtcgcg tcccggctcg tcgaacgcta cggacgtccc
     5101 accttcctca tcgggtggga tggcgatctg gggaagggat cggggcgcag cgtgctcggg
     5161 ttcgatctcc acggggcgct gcacgaggtg ggcgcctgtc tcgagaagta cggcgggcat
     5221 accatggccg cggggctcac catccagcgc ccccgattcg aggaattccg cgaaggcttc
     5281 ctggcggccg cggcccgcat ccttacaccg gacgatctgg ctcccgcgca gcgggtggac
     5341 ctggagctgc cgctgggctc ggtgagtgac gacctcgaac ggctgatgcg ccacctcgag
     5401 ccgtgcggcg cggggaatcc cgctccggta ttcggcgtgc ggggcgcgcg ggccgtcgac
     5461 gcgcggcgcg tgggcgcgaa tcacctcaag ttcaagctcg acgacggctc gggcgtgctc
     5521 gacgccatcg ggttccggtg ggccgatcag gtgcccgacg cctggctcgc cagtcccctc
     5581 gacgtggcgt tccggctcga gcgcgacgag tggcaggggc gcttccggct ccaggccaaa
     5641 gtcgcgtcga ttgcgccgag tgccgcggcg tgattcggag gtgtgcgaca ccgccctccg
     5701 tcgttcccac cctcaagggt gggcccgaac ggcatcacgc tttaagcgtt gtgcctcatc
     5761 gggcccaggc ttcagcctgg gaacgggcgt gatctggagg cgacgtgcga caccgccctc
     5821 cgtcgttccc accctcaagg gtgggcccga acggcaacac gcttcaagcg ttgtgcagtg
     5881 tcgggcccag gcttcagcct gggaacgaca ggcttcagcc tgggaacggc tgtgatcgcc
     5941 caggcttcag cctgggaacg ggcgtgatcg aaatcgtggc gggggagttc cggcgccgcc
     6001 ggttgaaaac gcccgccggc gacaagactc gtcccacatc gggacgggtg cgggaggcct
     6061 ggttcagcat cctccagact tcgattcccg gtgcccgtgt cctcgatctg ttcgcggggt
     6121 cgggggccct cgggctcgag gcgctgtccc gcggtgccac gagcgcggac ttcgtcgagc
     6181 gggcccgtcc cgccctcgac gccctccggg cgaacatcga gaccctgaag gtccaggatc
     6241 gggtgacggt gcaccgggcg gacgccctca agttcgtcga gcgtctccag ccggggcagt
     6301 acgacgtggc gttcgcggac ccgccctacg ccgaccccgc cgcggagcga ctctcggcgc
     6361 tgtttcgcac cgcgcctttc gcacgcatct ttgcggtgga gcactcggcc gacaccccgg
     6421 tgcagggtga cgacagccgc cgctacggcg acacggccat caccttcttc tacgcgtgac
     6481 gatgacccgg atcgcggtgt accccggctc cttcgacccc gtcacccggg ggcatgcgga
     6541 tctgatccgc cgctccctgg ccttttcgga ccaggtggtg gtggcggtgg cggtcaacgt
     6601 gtccaagcag ccgctcttta cattggagga gcgggtggcg ttgattcggg agacggtcaa
     6661 ggtgccgaac gtcgaggtcc ggtcgttcga cggactgctc gtcgacttcg cgaagcaggt
     6721 cggcgcatcg atcctgatcc gggggctccg ggccgtctcc gatttcgaat atgaatttca
     6781 gatggccctg atgaaccgca cgctggcgcc ggcactcgaa acggtgttcc tggttccggc
     6841 gttcgatctg acgtacctga gctcgagcct ggtgcgcgag gtcgcgcgct tcggcggaga
     6901 cgtttcggcc ctggtgtacc cggccgtgca gcaggccctg aaggcgaagt tcaaatcact
     6961 ctcggggagc aaggcatgac cttcagtatc aagaaggacg ccaaaggcgt cgtggtggtc
     7021 ggcgtggacg gccagctcat cgtcggcaac cgccaggagc tgaagcagaa ggtcctcgaa
     7081 tccctggagg gtggcgcccg caagttcgtg gtggatttct cgaccacggg ctacatcgat
     7141 tcgtcggggc tgggcgtcct ggtgtccctg tcgaagaaga tccgggagca gggcggcgat
     7201 ttgcggctcg ccgggctcaa cgaggatctg cagaccctct tcgagctcac caagctcgat
     7261 accctgttca ccatcgcgaa aacgcccgcc gaagccctcg ccgcgttctg aagtgaagat
     7321 cgcagtgcgg cgcgcggggt attcggggga cgtggagctc ctgctccgcg tcccgtcgga
     7381 cctgtcgttc gtcgcggaag cggtggacct cgtcgcctcg cacacgcctt ccgggattct
     7441 ctctccccgt cgcgtacagt tcaatctccg cacggtcctg gccgaggcac tcgcgaacgc
     7501 gatcacctac ggcaaccgtg aagaccccac caagaaggtc gaagtgcggg tcgaagcccg
     7561 gagtgagttc atccgcgtga ccgtcacgga ctccggggaa gggttcgatc cgtccgtcgt
     7621 gcccgacccc accgcacccg agggggtgga gcgggagttc gggcgcggac tcttcgtcat
     7681 ccgtcacctc gtcgacgccg tcgatttcaa cgccaaggga aacgccgtct gcctgaccct
     7741 gcgggcgggc tgactcgcct cgagcgtacc ctcgctgccc tgggcgccct cgccggggcg
     7801 aagctgcgcg tgcgcacggg taaagcggcc gccggccgcg ggtggaaggg gacggcggtg
     7861 ccggggttca cggacacctg gctcgaggtc aagagggcgg ggcgggggcg ggtgaacgcc
     7921 cgggcgctgg cgaagacggt cgccgccctg ctcgaggccg aagaagagac caaggcggtg
     7981 gcggccgagc tgtcggcgcg ctacgaagag atcgacctga tctacacgat cagcgaagtc
     8041 ctcgggcaca ccatccggct ttcggacgcc gcgtcccgga ttctccgcga ggtgtcggac
     8101 gtggtggggg cgcggcgcgc gaccctcctg gccgtggacg agggcggcaa ggtgctccgc
     8161 atcgtcgcgg cgcagggcat gaatccgtcg gaggcggagc cgatcgaggt ggacgatccg
     8221 tcgtcgatcg ccgcccgggt gttccgggag ggccggatcc tctcgtacaa cccgccggtg
     8281 ggggcggcga gcaccgccgc gaaccgggag tcccggtcgt accgcggcta cgcctacctg
     8341 tcggtgccgg ttctctacgc cacgcccggc ggcccgtccc gtcccatcgg cgtcatcaac
     8401 ttcacggacc gcatcggcgg agacgccttc acgacgggtg accggaagct ggtcgcggcc
     8461 atcgccaacc agatcggggc ggcgctggcg aacgcccggc tcgtgtcgcg ggatctcgag
     8521 caacagcggc tgcgccggga gctcgagctg gcgcgtgacc tacagctgaa gctcctgccg
     8581 tctccgctgg tgattgcggc caaggccgat gtcgcggccc ggtgccgccc cgccgaagtg
     8641 gtggggggcg atttctacaa tctgatgcgc ctgcccgggg accggatcgg cgtgatgatc
     8701 ggggacgtgt cgagccacgg gttcggcgcg gcgctgatca tggccctcgc catgtcggcg
     8761 tccggcattc acgcggagtc ggcgacgagc cccgcggcgg tgatgaccct gctcgaacag
     8821 tcgctggccg acgagctgca gaagaccgaa atgtttctca cgctcttcta cggggtgatc
     8881 gatccggcgg cggggaaact cttttacgcg aatgcaggcc acccccaggc gttcgtcgtc
     8941 cggtccgacg gcacgtcctg ccgcctcgaa gccacccgcc cgccactcgg cctcggggtc
     9001 gggggcaccg cggatgccga gcagccctgg gccaagcgcg ccgacaccct ctgtctgttc
     9061 accgacggaa tctcggacgc gatgtccctc cgcggcgtgc gcttcggcga agagcgcgtg
     9121 ctcggccacg tgagccggat gcgtgggcgt cccacgctgg acatcctcaa tgccgtgacg
     9181 acggagctcg acgcgttcac cgagggcgcc gaccccaccg acgaccgcac catcgtgctc
     9241 ctccgggtgt gaagcccaag aagagcctcg gccagcattt tctctcggac gtcgggatac
     9301 tcggcaggat cgcggacgcc ctggatctct cgcccggcga gaccgtgctg gagatcggcc
     9361 ccggccgggg ggatctcacg cggcaactcc tggctcgtgg cgcgcgcgtg gtggcggtcg
     9421 agaaggacga gaggcttgct gccgccctga cgggaaacgt gacacgggaa acgggaaacg
     9481 taagggtggt cgagggtgat gcgttggaga ttgactggca cccgctggtt cgggacacgt
     9541 ttcccgtttc tcgattcccg tttcccgact cccgattccc ttttcccggt ttttccgtca
     9601 tcggcaacat cccctactac atcacctctc ccttgctcga gaaggcgctc acaccgccgc
     9661 tcccgtcgcg catcgtgttc ctggtgcaga aggaagtcgc agatcgcatc gtggcgccgc
     9721 cggggtcccg gacctatggc gcgctgtcgg tggggatcca ggccgtctgc acggtcgaga
     9781 agctcttcgt ggtcaaggcg ggagcgtttc ggcccccacc gaaggtggac agcgcggtca
     9841 tccggctcac gccgctcgag cagccactcg tcccggtgga gcaggggacc gcgttccggc
     9901 ggttcgtcgt ggccgcgttc ggacagcggc gcaagcagct gcgaaacgtc ctcgccaggg
     9961 tgaccggcag gccggcggcg gcggtggccg atgaaatccg cgcgctgggg ttcgatcccc
    10021 agtctcgtcc ggagacactg tcgccggcgg atttcgcccg cctcttgatt tggagcaggc
    10081 aattgtgaaa taattcacaa tgcggaagat ttccgaatcc accgtccggc ggctgtccct
    10141 ctacctccag tatctggagc tgactcagga tcagggacgg accacggtgt cttccgaaga
    10201 gctcgcccgc cgttccggcg ccacctcggc ccaggttcgc aaagaccttt ccttcttcgg
    10261 ttccttcggg aagcgcggca tgggatacga agtccccgcg ctcatcacgc gcctccgcga
    10321 aatcctggga ctcgaacggg cgtaccgcgt ggtgctggtg ggagcgggga agatcggcgg
    10381 cgccctgatc cactacgagg gcttccggcg ccgcggcttc gaagtctccg ccatctacga
    10441 ccgcgacccc aagaagatcg ggtcccgttc gaatgggctg gtggtccggg acgtgaaaca
    10501 gctcgaagcg gatctcaaga aggagccgac ggacatcggc atcgtcgtga cgcccgggga
    10561 cgcggcgcag gaggtcgcgg accggctggt gcgggcaggg gtcaaagccc tgctcaactt
    10621 cgcgcccgtc cagctcaagg tgccgcggga cgtcgaagtg aagacggtca acatggcgca
    10681 ggagctcgaa gctctaagtt actccttggc aaatcgttag gctcgctcgt ttggggggtt
    10741 cacgcccccg gtagattcct cgcaccatgc catcggttcg cgttacctca gaaatcggtc
    10801 cgctgaaggc cgtcctcgtc catacccccg gtcccgagct ggtcgcggtg accccgggga
    10861 accgcgagga ctacctgtat gacgacatca tcgatctgga tctcgcccag cgtgagcacc
    10921 gccgcttcgt ggccgtgctg gagcgcttcg ccgaggtgta cgaggtccgg gatctcgtca
    10981 cggaggtggt cgcgcagaag gacgcgcggg acttcctggt cacccgcacg ctcgatgtcg
    11041 tcccttcaga cgcgctcgcc cagcggatgg cggagcttcc cgcggaggcc ctggtcggcc
    11101 tgatggtgga gggagcgctc gaagaagcgg gcccgatcgc ccgcgcgctg aatgaaggcg
    11161 gttacgcgct gcctcccctc ccgaatctct tcttcacgcg cgacaccggg atggtgatcg
    11221 gaaaccactg cgtgatcggc tcgatgcgct acggcgtccg gtggacggag gagctgctga
    11281 tcaagtcgct cttcaagtac caccccaagc tcgagaacgc cggcattctc tatgacgggt
    11341 ccgaggagaa gcggtcgaat tacacgctcg agggggggga cgtgcacccc atccggccgg
    11401 acctgatggt gctcgggttc tccgagcgct cgacgccggc cgcgctcgac cagctctgcg
    11461 acctcgcgtt cgagcagtgc ggcgtgacgg acgtcgtgat cgtggtgctc cccgcggagc
    11521 ggaccgcgat tcacctggac atgatcttca cccagatcga ccgcgaagcg tgctgcatct
    11581 atccaccgca cttcatcggc cccgagcgcc tggcggtgct gcatcgccgc aagaagtcga
    11641 agggcgtgaa ggaaatgccg aacttcttcg cggccatgca ggcggtgaat cagccgctcg
    11701 agccggtctt ctgcggcgga ccgtcccggt ccctgcagga gcgggagcag tggtcgtccg
    11761 cgtgcaactt cttcacggtg cgccccggcg tcgcggtgac gtacgcgcgc aacgaggcca
    11821 ccctgcgcga gttcgagcac gccggattcc ggatcatcag cgcggtgaac ttgctcaccg
    11881 gcgacgagta cctggaggac ggcgagcgcg ggatcatcac catcgaaggg tcggagctgg
    11941 tgcgtggagg cggcggtccc cggtgcatga cgctgcccct gcgcagagat gacttgtgac
    12001 cctccacacc accttccaga tcggccacct gaaggctcac gccatcgagt ccggctattt
    12061 gcggctcgac ggcgggtcga tgttcggcgt gatcccgaaa acactctggg agaagcacat
    12121 tccggcggat caacggaacc gcatccgcct ggcgatgcgg tgcctcctga tcgaacaccc
    12181 cgacggcctg gtgctgatcg ataccggatt gggcaacaag gaagacgcca agtttctgga
    12241 catttacggc gtggacaatg ggggcgtgga ccgtcgcacc cggctcgagg acggcatcag
    12301 ggaagcgggc catgccccgt ccgacgtccg gtgggtgatc aacacccacc tgcatttcga
    12361 tcacgccggc ggcaacacgt ggaaggatca catgggggag atcaacaaga ccttctccaa
    12421 ggcgacgtac gtcgtgcagc gccgggaata cgaattcgcg acccatacca acgagcggac
    12481 ccggggatcc tattttccgc tcaactggga gggcatcgac ttcacgctgc tgaatggcga
    12541 gcaggaagta ctcccgggca tcaagggcat cccgacgccc gggcacgttc ccggtcacca
    12601 gtcgatcctg gtggagagcg ggggagaaaa gctgctcttc atcgccgatc ttgttccgac
    12661 ggcgtcccac ctgccgttgg tctggatcat gggctacgac gtggaaccga tggtcaccct
    12721 cgaaacgcgg cggcacctct atccccgggc cgcggcggag gggtggcgcc tcttctttga
    12781 gcacgatgcg acgacgatcg aaggccgtct cgagaaggac gggaaggggt tcgcacttgt
    12841 ggccccggtg atgctcggct agcttttatg ccacaatgag ttaagcgggc gcgtcatcgc
    12901 gccccacccg cagccggcga gggaacgccg agcgccaggg ttgttggagc catcagccat
    12961 cagctatcag ccatcagcag actctgctga acgctgacgg ctgacggctg acagcttcca
    13021 gcgggccctg acgaaacaca gacggggccc gttttctttt cacgcggagt cgtcccactg
    13081 gcctacgaac ccggaaacag cagcagcaac accagcagca cgggaagtcc gagcagtacc
    13141 agcagcagcc gcacgccccg cacgcgggtg aaccgccaga tccggatcag tccggtgcgc
    13201 gtgatcgcgg acaatgggga tcagttggga gtgctgtcga tcgacgaagc gctcaacgcc
    13261 gccgcggagc ggggcctcga cctggtcgag gtggccccga tggcccggcc ccccgtggtc
    13321 aagatcatgg actacggcaa gtacaagttc gagcaggcca aggcggcgcg cgcggcgaag
    13381 aagaaacagc acgtgattca cttgaaggaa gtgaagttca ggcccggcat cgacgaccat
    13441 gactttcagt tcaagacccg ccacgcgcgg gagttcctgg aggaagggaa caaggtcaag
    13501 gtcacgatga tgtaccgcgg ccggcagatg gcccacatcg atctggggcg gcaggtgctg
    13561 gaccgggtgg ccgtggaatt gaaggacatc agcaagatcg agcaggaacc gaagctcgag
    13621 ggccggaaca tgtcgatggt gctaggaccg aaatgagagg gatcagggat caggggtcgg
    13681 gggtcagcaa tgcctcgaca actgttgatc cctggcccct gacccctgac tcctgaggtt
    13741 gaaatgccga agatgaagag caagcgcgcg ctgagaaagc gcgtgaaaat caccggctca
    13801 gggaagatcc ggcgtcaccg ggctttcaag agccacatcc tgaccaagaa acatcccaag
    13861 cggaagcgcc ggctccggca gggcgcgatg gtttcgcacg cggatcagaa gcgcctgatg
    13921 cgtctgctgc aggcgtaggg agagaccatg cctcgcgtca agagcaacgt cgcgcgcctc
    13981 gcgcgcaaga acaagatcat gaagctcgcc aaaggctact ggggcgggcg ttcgaaactg
    14041 tggaaggccg ccaaggagtc ggtggagcgc gcggagaagt acgcgtaccg cgaccgccgg
    14101 cgaaagaagg gcgacttccg gcgcctctgg atcacgcgga tcaacgcggc ggcccggctc
    14161 cacgagctct cgtacaaccg cctgatgaac ggcttgcgga aggcgggcgt cgagatcaac
    14221 cggaaagtcc tcgccgacat cgccgtccgc gatccggttg cgtttgaaca gctggcggag
    14281 gtcgcgaaga aggctctctg atatgcgcga cctcctcgac agcctcgccc ggctgcggga
    14341 gcgtctagcc gaaatccgcg aggtcgatgc gtcccggctc caggagctga aaaccgagct
    14401 cctgggccgc aaagccgggg cgctcaccga aatcctcaaa cagctcccca ccctcgaccc
    14461 cgagagccgc aaggccgtcg gggggcaggc gaacgccctc aaagctcagc tggaggacgc
    14521 catcgcaacg cgcgaacgcg cgctcgcgac tgccgcccgg aagcccaccg gcgtcgacct
    14581 cacgatgccg ggacgcccgg cgtggcgggg gggcctgcat ctcacgaccc aggtggtcaa
    14641 cgagatctgc ggcatcttcg ccgagctggg gttcacccgc gccgtcggac ccgaagccga
    14701 gaccgagcgc aacaacttct tcgcgctcaa cttcccgccc gaccacccgg ccctcgattc
    14761 ccaggattcc ttctatctgg gaaaggacac gctgctgcgc acccacactt cgccggtgca
    14821 ggtgcggacc ctcgaggcgt atccgccccc gattcgcgtg gtcattccgg gccgggtcta
    14881 tcgccgcgat cccttcgacg cgtcgcactc gcccgtcttc gagcagatcg aaggcctggt
    14941 ggtggacgag ggcgtgacct tcgtggatct gaaggccgcg ctgacggcgt tcgcccggcg
    15001 gttcttctcc ccgtccacga aagtgcgctt ccggccgtcg ttcttcccct tcaccgaacc
    15061 gtcggcggag atggacgtcc agcacccgct ctgcggcggc gcgggctgcc cgacctgcaa
    15121 gggcacgggc tggatggaga tcctcggctc cgggatggtg cacccggccg tgctcgagaa
    15181 gtgcggcgtg gacgcggagc gctacaccgg atacgccttc ggcatgggtc ccgagcggat
    15241 cgcgatgagc cgacacggca tcccggacat ccgtctcctc ttcggggacg acgtccgctt
    15301 cctctcccag ttctccgagg gagggaagcg atgaacgtgt cccgccgctg gctggaagcg
    15361 ctgctcgatc agcccctgga cgcgaaggag accgccgacc ggctctcgaa cctctgcgcg
    15421 ccggtggacg gcatcgtccc cctccatcac gatctcgggg acgtgctcat cgcgcacgtg
    15481 ctggaagtgc agaagcatcc gaatgccgat cgcttgagcc tgtgcaaggt cgacgcggga
    15541 ggcccggaag gccccgtcga agtggtctgt ggcgcgccga acgtgacggc ggggaagacg
    15601 tacccgtacg cgcccgtggg cgcgacgctc ccgggcggcc tgacgctcga gcgccggaag
    15661 atccgtggcg tcacctccaa cgggatgctc tgctccgcca aggagctggg gctgggccag
    15721 gatcatgacg gcatcctcga gctcgcgacg acggcgaagc ccggatcccg gttcctggac
    15781 gcgcttccca tcgcggacga acagatcatc atcgacgtcg gcgcgaaccg ccccgatctg
    15841 ctgtgccaca agggcgtcgc ccgcgagctc gccgtggcgt tcgggggccg catcaagttg
    15901 cccgcgattc ccggaagcac cggcgcggga gccgcgatgc ccgcaccgcg ccgcgccaca
    15961 accgaaggca aggtcggtgg cgtcaccatc acgctccagg ataccggcgg atgccgccgc
    16021 tatctcgccg cggtgattcg tggcgtgaaa gtggcgaaga gtcccgcctg gctctccgat
    16081 cgcctggagg ccgtcggcca gcggtccgtg aacaacgtcg tggacgccac caactacgtg
    16141 ctcttcgagc tgaaccagcc gatgcacgcg ttcgacctcg ccaagctccg gggccccgcg
    16201 gtcgtcgtgc ggcgggcgaa tgcgggcgag aggatcgtga cgctcgatgg ggtcgagcgg
    16261 accatgaccg ccgagatgac cgcgatctgc gacgccgagc ggccgaccat cgtcgcgggc
    16321 gtgatgggga gcgcggaatc agaagtcacg gaccagacga ccgacctggt gctcgagtgc
    16381 gcgtacttcg atcccacccg cacgcgccgc acgcgacggg ccctcggcct gtcgagcgag
    16441 tcgagctacc ggtacgagcg gggcatcgac ctgtggggca tgccggatgc gctccggcgc
    16501 gcgatcgacg tgatcaccgc ggtcgccggg gggacgctcg ccgacgctcc cctcgacctg
    16561 tggccggagc ccgagcagcc gcgcgcggtc ttcctgcgcc cggagcgggt gaatcacctg
    16621 ctgggcatcg aggtcccgcg aaccgaaatc gagaagctcc tcacgtcggt cggattcgtc
    16681 gcgttgccga aggatgaccg gatggcggtg caggtcccgg gctggcgtcc ggacgtgacc
    16741 cgcgaggtgg atctgatcga ggaggtcgcc cggctccggg gctacgactc cttcccggac
    16801 gagctccggg ccttccggcc cggcaccgtt cccgactccc cggtcgagca ggccaaggat
    16861 cgggtgcgcg aggtactggt gggggctggg ttctacgaag cccgcaccct ctcgctggga
    16921 cccgccaacg gtcccggggc ggtcgcggtc tccaaccccc tgtcggccga agacgcccat
    16981 ctccgcatgg ccctcctgcc ggccttgcgg cgccgggtgg aggggaactg ggccgagaag
    17041 aaccgggatg tgcgg
//
LOCUS       DASUBP010000200         4372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_373526, whole genome shotgun sequence.
ACCESSION   DASUBP010000200 DASUBP010000000
VERSION     DASUBP010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373526"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..774
                     /locus_tag="VFU75_07595"
     CDS             <1..774
                     /locus_tag="VFU75_07595"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5466739.1"
                     /translation="GLVVLRGRGLADTDSWAAPRVAVISATLAARDYKDGVAVGRKIL
                     VGRGAESGWYEVIGVVPDRSAEGIGGALAPAKAVYLSALQAPPARAELLADARPDRTI
                     VAAAGRVTGETSLTELRAREASVMAWFGRLTSAAGWAVMIIALAGMVAVMERWVTAVA
                     PELAIRRAVGARRWDVVRFILARAWLVCAIGAAGGFWLAIFVSGSLADAFPGLPAWSW
                     AAALAIGGALLAGTVVGAMVPGWRVVAVPPAAKLGALEG"
     gene            824..1087
                     /locus_tag="VFU75_07600"
     CDS             824..1087
                     /locus_tag="VFU75_07600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HEU5466740.1"
                     /translation="MPTYEYQCPSGHVFEKFSPTMTSAKTATCPKCGKRAKRLISGGA
                     GLVFKGSGFYITDYKRAGEKKPAESKPEPKPESKPSKTPSNDK"
     gene            1084..2715
                     /gene="argS"
                     /locus_tag="VFU75_07605"
     CDS             1084..2715
                     /gene="argS"
                     /locus_tag="VFU75_07605"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEU5466741.1"
                     /translation="MNAPDLIRAALMEAARRLGAPVDDVVLERPKDTSHGDLATNLAL
                     MLAKALKSKPRDVAQKLVDTVAFPAGVVRKIEIAGPGFINFFLAEQALASVIPVVIDA
                     GVRYGHSAGAGHPVNVEFVSANPTGPLHVGHGRQAALGDGIASLLEWTGWKVSREFYY
                     NDAGGQIDRLTQSVLARLQGQEPPTDGYHGDYIREVAEAYGSRRPSGSPLGLPAPDAV
                     RRFAVDYLRKEQDQDLQAFGVKFDTYFLESSLYTTRKVDDTLKTLERAGHTYRQDGAL
                     WLKTTDFGDDKDRVLVRSAEKGGEPTYFVPDVAYHVDKWERGFHRAINVQGADHHSTV
                     TRVRIGLQALDMGIPRGYPEYVLHQMVTVMKGGEELKVSKRAGSYVTLRDLLDEVGRD
                     AMRYFFLMRKSDSHFVFDVDLAKRQTEENPVYYVQMAHARMSGIFRVAGREPETARPD
                     GVDLAVLTQPEEVEILKDLAEFPAVVQGAADALEPHRIPGYLESLARVTHAWYHKYRV
                     LGEPEEAARLVLARAVRQVLANGLSLVGITAPDRM"
     gene            2717..3631
                     /locus_tag="VFU75_07610"
     CDS             2717..3631
                     /locus_tag="VFU75_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HEU5466742.1"
                     /translation="MSLLVVGSIALDSVQTPFGETADAPGGSAVFFAAAAAVLHPVQV
                     VGVIGSDYPLGVLKELTARGADLAGVEQVTGESFRWKGRYSYDLSSRETLETRLGVFA
                     DFRPNIPEKFRAAQYVFLGNIDPGLQLNVLDQVREPKLVACDTMNYWIQSRREVLLQV
                     LRHVDLVMVNDSEARELSGDWNVYRAAQWILEHGPRYCVIKQGEHGALLVGQEGTFKV
                     PAYPLQQVFDPTGAGDSFAGGFMAHLAATGDLSNENLRRAMVYGGAMGSFAVEAFGVQ
                     GFDKVTPADVRARARGFHELVRFDLGGA"
     gene            3632..>4372
                     /gene="purM"
                     /locus_tag="VFU75_07615"
     CDS             3632..>4372
                     /gene="purM"
                     /locus_tag="VFU75_07615"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEU5466743.1"
                     /translation="MTDRYGAAGVRLDVAEDAKRRIGALVATTRTPLALGKIGAFGGM
                     VRVPAGYQKPVLVLSTDGVGTKVLVAAQAGIHHTVGEDLVNHCVNDILVHGATPVAFL
                     DYIAAGVLDAAVAAALVEGIARGCRSHHMTLAGGETAQLPDLYAAGHYDLAGTIVGVV
                     DEDHALHGDHVAPGDVVVGYASNGLHTNGYTLARRIVFGEMGLGIDDPFPETSESVAQ
                     VLLAVHRSYAAAVMPVIARIHALAHITGG"
BASE COUNT          686 a         1382 c         1592 g          712 t
ORIGIN      
        1 ggattggtgg tccttcgggg gcgggggctc gcggacaccg acagctgggc cgcgccccgg
       61 gtggcggtga tcagcgcgac cttggcggcc cgcgactaca aggacggcgt cgccgtcggc
      121 cggaagatcc tggtcggccg gggagccgag agcgggtggt acgaggtgat cggggtagtg
      181 ccggaccgca gcgccgaagg gatcggcggt gcgctggcgc cggccaaggc agtctacttg
      241 agcgcgcttc aagcgccacc ggcgcgtgcc gagctccttg cggacgcacg tcccgaccgg
      301 accatcgttg cggcggcggg ccgcgtcacc ggtgagacgt cactcaccga actgcgagcg
      361 cgggaagcgt cggtgatggc gtggttcggg cgactgacca gcgcagcggg gtgggcggtc
      421 atgatcatcg cgctggcggg gatggtcgcg gtgatggagc gctgggtgac cgccgtggct
      481 cccgagctgg ccattcgtcg cgcggtcggt gcccgccggt gggacgtggt gcgattcatt
      541 ctggcccggg cctggctggt ctgcgccatc ggcgccgccg ggggcttctg gctcgccatc
      601 ttcgtctccg gctcactggc cgacgcgttc ccagggctgc cggcctggtc ctgggcggcg
      661 gcgctcgcga tcggtggtgc gttgctggcc ggcacggtcg tcggggcgat ggttccgggt
      721 tggcgggtgg tggcggtgcc gccggccgcg aaactgggag cgcttgaggg ctgaagtcgc
      781 atcgccttga ttcgagccgc cgccgcggct aagatgttgt gagatgccca cctacgaata
      841 ccagtgccca tccgggcacg tgttcgaaaa gttttcgccc acgatgacga gcgccaagac
      901 cgcgacctgc ccgaagtgcg gcaagcgcgc caagcggctc atctcggggg gcgccggcct
      961 cgtcttcaag gggtcggggt tctacatcac cgactacaag cgggcagggg aaaaaaagcc
     1021 ggccgagtcg aagccggagc ccaagcccga atcgaagcct tccaagaccc cttcgaacga
     1081 caagtgaacg ccccggacct catccgtgcg gcattgatgg aagccgcgcg ccggctgggc
     1141 gcgcccgtgg acgacgtcgt cctcgagcgc cccaaggaca cgagccatgg ggacctcgcc
     1201 accaacctgg cgctgatgct cgccaaggcg ctcaagtcga agccccggga cgtcgcgcag
     1261 aagctggtcg acacggtcgc gttcccggcc ggtgtggtcc ggaaaatcga aatcgccgga
     1321 cccgggttca tcaatttctt cctcgccgag caggcgctgg cgtcggtgat tccggtcgtg
     1381 atcgacgccg gggtgcggta cgggcacagc gcgggtgccg gccatccggt gaatgtcgag
     1441 ttcgtctccg cgaatccgac ggggccgctc catgtcggcc atggccggca ggcggcactg
     1501 ggggacggga tcgcgtcgtt gctcgagtgg accggatgga aggtgagccg ggagttctac
     1561 tacaacgacg ccggcggcca gattgaccgg ctgacgcaga gtgtcctggc ccgcctccag
     1621 ggccaggaac ccccgaccga tggttaccac ggcgactata tccgcgaagt agcggaggcc
     1681 tacggctccc ggcgaccgtc agggagtcct ttagggctcc cggctcccga tgcagtccgc
     1741 cgcttcgccg tcgattatct ccggaaagag caggatcagg atttgcaggc gttcggcgtg
     1801 aagttcgaca catattttct ggagtcgtcg ctgtacacga cccggaaggt cgacgacacg
     1861 ctgaagaccc tcgagcgcgc cggccacacc taccggcaag atggcgcgct ctggctcaag
     1921 accaccgact tcggggacga caaggaccgc gtactggtcc gcagcgccga gaagggcggg
     1981 gagccgacgt acttcgtgcc ggacgtcgcg taccacgtcg acaagtggga gcgcggcttc
     2041 caccgggcca tcaacgtaca gggggcggat caccacagca cggtgacccg cgtgcgcatc
     2101 ggactgcagg cgctcgacat ggggattccg cggggatatc cggaatacgt cctgcaccag
     2161 atggtgacgg tgatgaaggg cggcgaagag ctgaaggtct cgaagcgcgc cggctcctac
     2221 gtgacgctgc gggacctgct ggacgaagtc ggccgcgacg ccatgcggta cttcttcctg
     2281 atgcgcaaga gcgattccca cttcgtgttc gacgtggatc tcgccaagcg gcaaaccgag
     2341 gagaatccgg tctactacgt gcagatggcg cacgcgcgga tgagcggcat tttcagggtg
     2401 gcgggccgtg agcccgagac tgcccgtccc gacggcgtgg atctcgccgt gctcacgcag
     2461 ccggaagagg tcgagatcct gaaggatctg gctgagtttc cggccgtggt gcagggagcg
     2521 gcggacgccc tggagcccca ccgcattccc gggtatctcg agtcgctcgc ccgggtcacc
     2581 cacgcctggt atcacaagta ccgcgtgctc ggcgaaccgg aggaggccgc gcggctggtg
     2641 ctcgcccgcg cggtgcggca ggtgctggcg aacggactct ccctggtggg catcaccgcc
     2701 ccggatcgga tgtgagatga gtcttctcgt cgtgggctcc atcgcgctcg actcggtgca
     2761 gactcctttc ggcgagaccg ccgacgcgcc gggcggctcc gccgtgttct tcgccgcggc
     2821 cgcggccgtc ctgcatccgg tccaggtcgt gggcgtgatc ggttcggact atccgctcgg
     2881 cgtcctgaaa gagctgacgg cgcgcggcgc ggatctggcg ggcgtggagc aggtaacggg
     2941 cgagtccttc cggtggaagg gcaggtattc gtacgacctc tcgagccggg aaacgctcga
     3001 gacgcgcctc ggagtgttcg ccgacttccg tcccaacatc ccggagaaat tccgggcagc
     3061 gcagtacgtg ttcctcggca acatcgaccc ggggcttcag ctgaacgtcc tcgaccaggt
     3121 ccgtgaaccg aagctcgtcg cctgcgacac catgaactac tggatccagt cgcgccgcga
     3181 ggtgctgctg caggtcctgc gtcacgtgga cctcgtgatg gtgaatgact ccgaggcgcg
     3241 tgagctgtcg ggggactgga acgtgtatcg cgcggcgcag tggattctcg aacatgggcc
     3301 ccggtactgc gtcatcaagc agggcgagca tggggcgttg ttggtggggc aagagggaac
     3361 cttcaaggtc cccgcgtatc cgctacaaca agtcttcgac ccgaccgggg cgggagactc
     3421 gttcgccggc ggcttcatgg cgcacctcgc cgccaccggt gacctgtcga acgagaatct
     3481 ccggcgggcc atggtgtacg gcggcgcgat gggatcgttt gccgtcgaag cgttcggcgt
     3541 acagggattc gacaaggtga ccccggcgga tgtacgcgcc cgggcgcgag ggttccacga
     3601 gctggtgcgg ttcgatctcg ggggggcttg agtgaccgat cggtatgggg cggcgggggt
     3661 ccggctcgac gtcgccgaag acgccaagcg gcgcatcggc gcgctggtgg ccacgacgcg
     3721 cacgccgctc gcgctcggga agatcggcgc gttcggcggc atggtgcgcg tgccggcggg
     3781 gtaccagaag cccgtgctgg tcctgtcgac ggatggggtc gggaccaagg tgctggtcgc
     3841 ggcccaggcg ggcatccacc acacggtggg cgaggacctc gtcaatcact gcgtcaacga
     3901 catcctggtg catggggcga cgccggtcgc gtttctcgac tatatcgcgg ccggtgtgct
     3961 cgacgccgcc gtggccgccg ctctggtgga ggggatcgcg cgggggtgcc ggtctcatca
     4021 catgaccctg gcgggcggtg agacggcgca gctgcccgac ctgtacgccg caggacacta
     4081 cgacctggcg ggcaccatcg tcggcgtggt ggatgaggac cacgccctgc atggcgacca
     4141 cgtcgcgccc ggcgacgtgg tggtaggcta cgcgtcgaac ggactccata ccaacggtta
     4201 caccctggcg cgccgcatcg tgttcggtga aatgggactg gggatcgacg atccgttccc
     4261 ggaaacctcc gagtccgtgg cgcaggtctt gcttgcggta caccggagct acgcggcggc
     4321 cgtgatgcct gtcatcgccc ggatccacgc gctggcgcac attacgggcg gc
//
LOCUS       DASUBP010000201         9523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_414040, whole genome shotgun sequence.
ACCESSION   DASUBP010000201 DASUBP010000000
VERSION     DASUBP010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9523
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_414040"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1434)
                     /locus_tag="VFU75_07620"
     CDS             complement(<1..1434)
                     /locus_tag="VFU75_07620"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5466744.1"
                     /translation="MTKMFLSLATLCACAAFSLPAQQGDTLHVHGDSLNHHPITLEAI
                     TVTAAPEKRDEPASTVKIRPAAILQTPATDAYDLLRQLGGIDVHEQGQGPGFASDASV
                     RGFSSDHSTDIALWVDGVPNNEPINGHAEGYNDWNLLFPEAISGIDILKGPTNATYGN
                     FAFSGVVNVQTLERTNTTSLWLSPGSYGRFDGGFLTGYDHGTNGAVFGVRGVREDGWR
                     PNSGYDVGQVHARLVQGLSESTTLDAGFEYYTTHWRSPGFLSDSAFEAGLFGSAANPT
                     DAGNKDRAQERVSLRVLGSSSLLWRTTLYSTQGHFHWFLTTPPEGSVGEGTGSQTEEA
                     DLRYGFGLVSALTWYLPRAEITVGTEEGYAHSRYSNWFTTNRVEDSAQVLATAHQSSA
                     ALFLQSTEDLGYHFRLNLGGRFDLQNNESTPDGGPSVSHGKSIFSPKVGLLYHLPVAA
                     DLYANVSRGFRQTDGVIEDPTLPFITVW"
     gene            complement(1508..1873)
                     /locus_tag="VFU75_07625"
     CDS             complement(1508..1873)
                     /locus_tag="VFU75_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466745.1"
                     /translation="MTHRRAPLLALVLSLALVAYPATMPIAMAGEMPAPMPTPMHMAG
                     EGASTHTHHPAHHHHALSACCAGVCGSCPVVSVAAGVPPRIRASNRVVLFQNDLPTSP
                     QQSAARKLPYSIGPPASLA"
     gene            1952..3199
                     /gene="icd"
                     /locus_tag="VFU75_07630"
     CDS             1952..3199
                     /gene="icd"
                     /locus_tag="VFU75_07630"
                     /EC_number="1.1.1.42"
                     /inference="COORDINATES: protein motif:HMM:TIGR00183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate dehydrogenase (NADP(+))"
                     /protein_id="HEU5466746.1"
                     /translation="MALPLREEVAVAVAGGKIDYQGGRWIVPDHPIIPFIEGDGTGAD
                     IWRASQRVFDAAVAKAFGGKRSIVWKEVLAGEKAFKATGNWLPDETLAVIREHRVAIK
                     GPLTTPVGGGMRSLNVALRQILDLYACIRPVRWFAGVPAPVREPQKLDVVIFRENIED
                     VYAGIEFEAGSEKADKLRDFLIDQLGVKTLRQGSAIGIKPMSKFGSQRLVAKAIQYAI
                     DHQRPSVTLVHKGNIQKFTEGAFRDWGYQLAKESFGGQTIPEADAGSGTREAGRVLIK
                     DRIADAMFQQVLLRPDEYSVIATSNLNGDYLSDACAAQVGGLGMAPGANVGDGYAVFE
                     ATHGTAPKYAGQDKVNPGSVILSGVMMFEEIGWSAAGKAIIAGIEGALAAKKVTYDLA
                     RQMPGATEVPTSTFADAIIAHLK"
     gene            3196..4689
                     /locus_tag="VFU75_07635"
     CDS             3196..4689
                     /locus_tag="VFU75_07635"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HEU5466747.1"
                     /translation="MNSTVVTTPLASLETPLLAVIVPSGNALSPALADLDRATGGTLG
                     RAISTGDYKGKKDETALFYGTGPAQRVLLVGVGKAGDVSRSSLRRAAAVAGKRARGVG
                     VPRFAIAVAPEVRSNVPARDVGQVLAEGTSHGSWAFTEMKKPPEEPRVEVDSVTIVAN
                     AAEQKDVEAGRVVGAAIAEGSRFTRWLQMLPGNRCTPSFLAEQARELAKKHGQVVTTL
                     DLAAIKREGMGALLAVAQGSAEEPRFIILEYQGGAKGAAPVVLVGKGVTFDSGGISIK
                     PAANMEEMKYDMSGAAAVLGTFEALGRLKPAINVVGVIPATENLPSGTAVKPGDVVTS
                     YSGKTIEIINTDAEGRLILCDALWFVKRLKPAAVIDAATLTGAVTVALGNQATGLMGN
                     DDALMTEVQAAGERAGERCWPFPLWEEYHDLLKSEIADVKNSGGRGAGTIAGGWFLKE
                     FVDGYPWVHLDIAGTAYTDGEGPHQTKGPTGMGVRLFSEFVLRRAGA"
     gene            4878..6518
                     /locus_tag="VFU75_07640"
     CDS             4878..6518
                     /locus_tag="VFU75_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466748.1"
                     /translation="MMPTTVPTGALPRGDRYVFPHDSIVFSNIVTLSDLLNHIPGVFV
                     ARPGFYGATEPVLYAGRGPAALEVYLDGVPYVPLGRDSVWLDPARIPLGPMERVEVQV
                     LPGSLRVYLVTSRQRSTFATSEVDVKTGQFSTSGYRGGFAKRWLSGLGLSLGADYNNI
                     NGDPGTTTTPFNSVDVWLKGEYIPSPHYGATYQLIASSWERSRQTQGVTDTALAIQHW
                     KSKRSDVMFRAFYAQREDGLGWRVQTALAATTTSRDTALVDRSMWQDQFDVSETMSRA
                     ALGVTATLSDRTWPLRFDGRASWMPLPWITLAADARHSAYRGGRHGTRAHGSAGVTLP
                     LGFSLRGDYAWAQDLQAPALKQDSIRKTIDFSGAVRWDSRFASVDVGAAQLDPFQPIG
                     FALGIPAARSLAPTPRTTVATFQASVRPYPGFTLSGWYYQPRVGGGAFMPPTHGRYSL
                     TFDSKFWRVFRSGIFRLRAEYAAESWSRSPLGAINTSGQQPLHGATFTETNLEMQIAG
                     VVVFWQIRNNNYMRSQYVVGTDYPKNVQVYGVRWTFNN"
     gene            6553..7431
                     /locus_tag="VFU75_07645"
     CDS             6553..7431
                     /locus_tag="VFU75_07645"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HEU5466749.1"
                     /translation="MPRSMTGFGSADGAVAGGRLRIDIRTVNHRYYNPQLKLPGELSG
                     AEGELRERLRQLLDRGHVAVSARWVEAPAGVDGVTVDLDRARAVVNAMRDLKKKLRLK
                     GEPDVGLVARHPEVLRSGGNGGTPDIGWADIQPIVEAAAKDVLAMREREGKALARDLL
                     GRLDALEAGAAKIEARAPQRLTAEMARLKASVAELAAGVRVDEQRLALEIALMADRVD
                     IMEELVRFRTHVAACRDALKGSAAVGKQLGFLAQELLREVNTMGSKANDAEITQTVIA
                     MKGELERFREQLENLE"
     gene            7428..8081
                     /gene="gmk"
                     /locus_tag="VFU75_07650"
     CDS             7428..8081
                     /gene="gmk"
                     /locus_tag="VFU75_07650"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEU5466750.1"
                     /translation="MNPKVVVLSSPSGGGKTTIAKRLLSDWPKEFGYSVSATTRAPRA
                     GEVDGEAYHFLSRAEFDARVAAGAFLEWAEYAGERYGTLKAEVDRVLGTGRHVVLDIE
                     IQGARAVRKAYPRPASVAIFILPPSAAALVSRLTFRRTDRIGKIRERLEIARGEVLES
                     KTYDYVVLNDQLDLAVRAVASIARDGKGVPERPSQYQALVNEFLTGLSEALEQSGAE"
     gene            8091..8303
                     /locus_tag="VFU75_07655"
     CDS             8091..8303
                     /locus_tag="VFU75_07655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466751.1"
                     /translation="MRIITPIEVANQTQNKYLGVLVAAKFARFVNEFPRDRSVEFEEK
                     LTTRALQELVQGHLRFKMVRRRRSES"
     gene            8303..9493
                     /gene="coaBC"
                     /locus_tag="VFU75_07660"
     CDS             8303..9493
                     /gene="coaBC"
                     /locus_tag="VFU75_07660"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HEU5466752.1"
                     /translation="MRLAGRHVVLGVSGGIACYKSCILARRLVEDGATVDVVMTQAAT
                     EFVRPLTFEALTGRKVITSLWEPGRALAHVDLAKHPDLVIVAPATAQLLARAAQGLAD
                     DFLTSLLIARSAPILAAPAMNDGMYAHPATQANLQTLRDRGWHFVGPDVGALAEGPSE
                     RPGRMSEPEAIVSEVRRILAPAGPWRGRHVVVTAGPTREHLDPVRVITNPSSGRMGYA
                     LAEAALARGAVVTLVAGPTDLPPPSGATVVRIESTEDLRHEIQRVLGGASALFMAAAP
                     ADFKPAKADKTKRARSKGKLTLTLAPTPDVLQSLKRPKDCLMVGFALETGDGMKHAER
                     KLKEKKLDAIVLNDATEPGAGFEVSTNKVTIITRDGDVQALPLLSKREVADKILDAVE
                     SKLS"
BASE COUNT         1627 a         3033 c         3336 g         1527 t
ORIGIN      
        1 ccacaccgtg atgaagggga gcgtggggtc ttcgatcacg ccgtccgtct gccggaatcc
       61 ccgggacacg ttcgcgtaga gatccgccgc cacgggaagg tggtagagca gcccgacctt
      121 gggggaaaag atggacttgc cgtggctcac cgacgggccg ccgtcgggag tgctttcgtt
      181 gttctgcaaa tcgaaacgcc cgccgaggtt cagccggaag tgatagccga ggtcttcggt
      241 ggattggaga aacagggcgg cggaggactg atgggcggtc gccagcacct gcgccgaatc
      301 ctccacccgg ttggtcgtga accagttcga atacctcgag tgcgcgtacc cctcctcggt
      361 gcccacagtg atttccgcgc ggggcaggta ccaggtcaac gcactgacca gtccgaatcc
      421 atagcgaagg tccgcctctt ccgtctggct cccggtgccc tcgccgacac tgccctcggg
      481 cggcgtggtg aggaaccagt ggaagtggcc ctgggtggag tagagggtcg tgcgccacag
      541 gagcgacgag ctgccaagga cgcggaggct gacgcgttcc tgcgcgcgat ccttgttgcc
      601 ggcgtcggtc gggttggcgg cgctcccgaa gagcccggct tcgaaggcgc tgtcggaaag
      661 gaagcccggg gaccgccagt gcgtggtgta gtactcgaat cccgcgtcga gagtcgtgct
      721 ctccgatagt ccctgcacga ggcgagcgtg aacctggccc acgtcgtagc cgctgttcgg
      781 ccgccacccg tcttcgcgta ctccacgcac gccgaacacg gccccgttcg tgccatgatc
      841 gtatcccgtc aggaagccgc catcgaaccg cccataggaa cccggggaga gccacagtga
      901 cgtcgtgttg gttcgctcca gggtctggac gttcacgacg cccgaaaacg cgaagttgcc
      961 gtaggtggcg ttcgtcggcc ccttgaggat gtcgatcccc gaaatcgcct caggaaagag
     1021 caggttccag tcgttgtaac cctcggcgtg cccgttgatg ggctcgttgt tcggaacgcc
     1081 gtccacccag agcgcgatat ccgtcgagtg atcggatgag aagccgcgga ccgaggcgtc
     1141 ggacgcgaag cccgggccct gtccctgctc gtgcacgtcg atgcctccga gctggcggag
     1201 cagatcatac gcgtcggtgg ccggcgtctg cagaatggcg gcgggccgga tcttcaccgt
     1261 gctggccggc tcgtcccgtt tttccggcgc ggccgtcacg gtgatggctt caagggtgat
     1321 ggggtggtga ttgagactgt cgccgtgcac gtgcagcgta tcgccctgct gtgccggcaa
     1381 ggaaaacgcg gcgcacgcgc agagcgtggc caggctcaag aacatcttcg tcatgtatgt
     1441 cctcacgcga gttcccggaa cggacgaacg tccgtgccgg aggtcagacg gcccagttgt
     1501 ggatcagcta ggcgaggctg gcgggaggac cgatggaata agggagcttg cgggctgcgg
     1561 actgctgagg cgatgtgggc aggtcattct ggaacaggac gacccggttc gacgcgcgga
     1621 tccgcggcgg tacgcccgcg gccaccgaaa cgacagggca cgatccacat acgccggcgc
     1681 agcaggcgct gagggcatgg tggtggtgcg ccgggtgatg ggtgtgggtg gacgcgccct
     1741 cgccagccat gtgcatcggc gtcggcatgg gggccggcat ctcgcccgcc atggcgatcg
     1801 gcatcgtggc gggatacgcc acgagcgcca gggagaggac gagggcaagg aggggcgcgc
     1861 gtcggtgcgt cattggtgga gggaatgatt cctcgtaccg ggccgctggc gcaacccctc
     1921 taaccgtata tagactgcaa gcttcttgtg aatggccctc ccccttcgag aagaggtcgc
     1981 ggtggctgtc gccggcggca aaatcgacta ccagggtggc cgttggatcg ttccggacca
     2041 tcccatcatc ccattcatcg aaggcgatgg taccggggcc gacatctggc gggcctctca
     2101 gcgcgtcttc gacgccgccg tggccaaggc gttcggtggc aagcggtcga tcgtctggaa
     2161 ggaagtcctc gccggcgaga aagcgttcaa ggcgaccggg aactggctgc ccgacgaaac
     2221 gctcgcggtg atccgggagc accgggtcgc catcaagggg cccctgacca cccccgtggg
     2281 cggtgggatg cggtcactga acgtcgccct ccgccagatc ctcgacctgt acgcctgcat
     2341 ccgcccggtg cgctggtttg ccggcgttcc ggcgcccgtc cgggagccgc agaaactgga
     2401 cgtcgtgatc ttccgggaga acatcgaaga cgtctacgcg gggatcgagt tcgaggccgg
     2461 gagtgaaaag gccgacaagc tccgtgattt cctgatcgac caactcggcg tcaagacgct
     2521 ccggcagggg agcgccatcg gtatcaagcc gatgtccaag ttcggctccc agcggctggt
     2581 cgcgaaggcc attcagtacg ccatcgatca ccagcggccg tcggtcacgc tggtacacaa
     2641 gggcaacatc cagaagttca ccgagggcgc cttccgggat tggggctatc agctcgcgaa
     2701 ggagagcttc gggggtcaga ctatcccgga ggcggacgcg ggaagcggga cgcgggaagc
     2761 cggaagagtg ctgatcaagg accgcatcgc cgatgcgatg tttcagcagg tgctgctccg
     2821 gcccgacgag tactcggtca tcgcgacgtc gaatctgaac ggcgactacc tctccgacgc
     2881 ctgcgccgcg caggtcggcg gccttggcat ggcgccgggg gcgaacgtgg gcgacggcta
     2941 cgccgtgttc gaagcgaccc atggcacggc gcccaagtat gcgggtcagg acaaggtcaa
     3001 ccccggcagc gtcattttgt ccggcgtcat gatgttcgag gagatcggct ggagcgccgc
     3061 cgggaaggca atcatcgccg gtatcgaggg cgcgctcgcc gcgaagaaag tcacgtacga
     3121 tctcgcccgg caaatgcccg gcgcgactga agttccgacc tccacctttg ccgacgcgat
     3181 catcgcgcac ctgaaatgaa cagcaccgtc gtcaccaccc cactcgcgtc cctcgagaca
     3241 cccctgctgg ccgtgatcgt cccctcgggg aacgcgctct cgccggctct ggctgacctg
     3301 gaccgcgcaa ccgggggcac gctggggcgg gcgatctcga ctggggatta caaaggaaag
     3361 aaggatgaga cggcgttgtt ctacggcacc ggccccgcgc agcgggtcct gctggtcggt
     3421 gtcgggaagg cgggcgacgt gtcccgctcg tcgttgcgcc gcgcagccgc cgtcgcggga
     3481 aagcgcgccc ggggagtggg ggtgcccagg ttcgcgatcg ccgtggcccc cgaagtccgg
     3541 agcaacgtgc cggcgcgcga cgtcggtcag gtgctggcgg agggcacgtc tcacggatcg
     3601 tgggccttca cggagatgaa gaagccgccc gaggagccgc gcgttgaggt cgactcggtc
     3661 accatcgtgg cgaacgccgc ggagcagaag gacgtggaag cgggacgggt cgtcggtgcc
     3721 gcgatcgccg agggatcccg gttcacccgg tggctccaga tgctccccgg caacaggtgc
     3781 acgccgtcgt tcctcgccga acaggcgcgc gagctggcca agaagcacgg acaggtcgtc
     3841 acgacactgg acctcgcggc gatcaagcga gaaggcatgg gcgcgctcct ggccgtggct
     3901 cagggctccg ccgaggagcc gcggttcatc atcctcgagt atcagggtgg ggccaagggc
     3961 gccgcgccgg tggtgctggt ggggaagggc gtgacgttcg actccggcgg tatttcgatc
     4021 aagccggcgg cgaacatgga ggaaatgaag tacgacatgt cgggcgccgc cgccgtcctc
     4081 ggcacgttcg aggcgctggg acgcctcaag cccgccatca acgtggtcgg cgtgattccc
     4141 gcgaccgaga acctcccctc cggcacggcc gtgaagcccg gcgacgtcgt caccagctac
     4201 tccggcaaga cgatcgagat catcaatacg gacgccgagg gacgcctcat cctgtgtgac
     4261 gcgttgtggt tcgtgaagcg gctcaagccg gcggcggtga tcgacgcggc gacgctcacc
     4321 ggcgcggtga ccgtggcgct cgggaatcag gccacgggac tgatggggaa cgacgacgcc
     4381 ctgatgacgg aagtgcaggc ggcgggggaa cgggccgggg agcggtgctg gccctttccg
     4441 ctctgggagg agtatcacga tctcctcaag tcggagatcg cggacgtgaa gaactcgggt
     4501 ggacgcgggg ccgggacgat cgcgggtggg tggtttctca aggagttcgt ggacggctat
     4561 ccgtgggtcc atctggacat cgcggggacg gcctacactg acggtgaggg accccatcag
     4621 accaagggac ccaccggcat gggggtgcgc ctcttctccg agttcgtcct acgtcgagcc
     4681 ggcgcgtaaa gctcgcgctg gctctcgccg cgggcgtcgc gctcgcggcg cctcttcgcg
     4741 cacagagcga caccaccgga agaccggtcc gcccgccggc cgacacctcg cacgccccgg
     4801 gcgacacgac cccgccggat accctgggtg ccaggaagga caccacgccg aaggacacgg
     4861 tcgcgcacta tctgccggtg atgccgacca cggtgccgac tggagcgttg ccgcgcggtg
     4921 accggtatgt cttcccgcac gactcgatcg tcttctccaa catcgtcacg ctttcggatc
     4981 tgctgaatca cattcccggc gtgttcgtcg ctcgaccggg gttctacggc gccaccgagc
     5041 ccgtgctcta tgcgggccgg ggacctgccg cactcgaggt gtatctcgac ggtgtcccgt
     5101 acgtgccgct gggacgcgac tcggtgtggc tggatcccgc ccggattccg ctgggtccca
     5161 tggagcgggt ggaagttcag gtactccccg gatcgctccg cgtctacctt gtcacgtcac
     5221 gccagcggtc gaccttcgcg acgagcgagg tggacgtaaa aacgggacag ttctccacca
     5281 gcggctatcg cggcggcttc gcgaaacgct ggctctcggg gctcggtctg tccctcggtg
     5341 ccgattacaa caacatcaac ggcgatccgg gaaccacgac gacgccattc aattccgtgg
     5401 acgtgtggct caagggcgag tacatcccga gcccgcacta cggggccacg tatcaactca
     5461 ttgcgtcgag ctgggagcgg agtcggcaga cgcagggtgt gacggacacc gcgcttgcca
     5521 tccagcattg gaagtcgaag cgctcggacg tgatgttccg cgccttctac gcccagcgcg
     5581 aagacggact cggctggcgg gtgcaaaccg cgctcgccgc cacgacgacc agccgtgaca
     5641 cggcgctcgt ggatcggtcc atgtggcagg accagttcga cgtcagcgag acgatgagcc
     5701 gggctgccct cggggtgacc gccacgctct cggaccggac ctggcctctg cggttcgatg
     5761 ggcgcgcctc ctggatgccc cttccttgga tcactctcgc ggctgacgcg cgtcactcgg
     5821 cataccgcgg cgggcggcat ggtacccgcg cccatggttc ggccggggtc acactgcctc
     5881 tgggattctc gttacgaggg gattacgcct gggcgcagga tctccaggcg cccgccctga
     5941 agcaggattc gatccggaag acgatcgact tctcgggcgc ggtgcgatgg gacagccgtt
     6001 tcgcatcggt cgacgtcggg gccgcccagc tcgatccgtt ccagccgatc ggcttcgcct
     6061 tggggatccc cgcggcccgg tctctcgcgc cgacaccgcg aaccaccgtg gccacgttcc
     6121 aggccagcgt caggccctat cccgggttca cgctgtccgg ctggtactac cagccccgcg
     6181 tcgggggtgg cgctttcatg cccccgaccc atggccggta ctccctgacg ttcgattcca
     6241 aattctggcg ggtgttccgg agcgggattt tccgcctgcg cgcggagtat gccgcggagt
     6301 cgtggagccg ctcgccgctg ggggccatca acaccagcgg gcaacaaccc ctgcatgggg
     6361 ccacgttcac ggagaccaac ctggagatgc aaatcgccgg ggtggtggtt ttctggcaga
     6421 tccgcaacaa caactacatg cggtcccaat acgtcgtggg caccgactat cccaagaatg
     6481 tgcaggtcta cggagtacgc tggaccttca ataattgacc aataatcggc tccccgggta
     6541 gctttctgga ctgtgccaag aagcatgacg ggattcggtt ccgctgatgg agcggtggcg
     6601 ggaggacggc tgcggatcga catccgcacc gtcaatcacc gctactacaa tccccagctc
     6661 aagctgccgg gcgagttgtc cggcgccgag ggcgagctgc gcgagcggct ccgccagctg
     6721 ctcgaccgcg gccatgtcgc ggtgtccgcg cggtgggtgg aagcgccggc gggggtcgac
     6781 ggcgttacgg tcgacctgga ccgggcccgc gccgtggtca acgcgatgcg ggatctgaaa
     6841 aagaagctcc ggttgaaagg cgagccggac gtcggactgg tggcgcggca tccggaggtc
     6901 ctgcggtcgg gcggcaacgg cggcacgccg gacatcggat gggcggatat tcagcccatc
     6961 gtcgaagccg cggcgaagga cgtcctcgcg atgcgggagc gggaagggaa ggccctcgcc
     7021 cgcgacctcc tgggccggct cgacgccctg gaggccgggg ccgcgaagat cgaagcccgc
     7081 gcaccgcaac ggctcacggc cgagatggcc cggctgaaag cgtccgtggc ggagctcgcg
     7141 gcgggagttc gcgtggacga acaacgcctg gccctcgaaa tcgccttgat ggccgatcgc
     7201 gtcgacatca tggaagagct ggtacggttc cggacccacg tcgccgcgtg ccgcgacgcg
     7261 ctgaagggtt cggccgcggt cgggaagcag ctgggattcc tcgcgcagga attgctccgc
     7321 gaggtgaaca cgatgggctc caaggcgaac gatgccgaga tcacccagac cgtgatcgcc
     7381 atgaagggcg agctggagcg tttccgggag cagctcgaga acctcgagtg aaccccaagg
     7441 tcgtggtgct ttcgtctccg tcggggggcg gcaagacgac cattgcgaag cgactcctgt
     7501 ctgattggcc caaggagttc ggctattcgg tgtcggccac gacccgggcg ccgcgtgccg
     7561 gcgaggtgga cggggaggcc taccacttcc tgagccgggc cgagttcgat gcccgggtgg
     7621 cggctggtgc gtttctggag tgggcggagt atgccgggga gcgatacggg accctgaagg
     7681 cggaagtcga ccgggtattg ggcaccgggc ggcacgtggt actggacatt gaaatccagg
     7741 gtgcccgggc cgttcggaag gcataccccc ggcccgcctc tgtcgccatc ttcatcctcc
     7801 cgccaagtgc cgcggcactg gtgagccggc tgaccttccg gcgcaccgac cggatcggga
     7861 agatccggga gcggctggag atcgcgcggg gggaagtgct cgagtcgaag acatacgatt
     7921 acgtggttct gaacgatcag ctggacctgg cggtgcgcgc ggtggcgagc atcgcgcgtg
     7981 acgggaaggg cgtgccggag cggccgtcac aatatcaagc gctcgtaaac gagttcttga
     8041 ccggactctc agaagccctg gagcagagcg gggccgaata aggagcagca atgcggatca
     8101 ttaccccgat tgaagtcgcg aaccagaccc agaacaagta tctgggcgtg ctggtggcgg
     8161 cgaagttcgc gcgattcgtg aacgaattcc cccgggaccg ctccgtggag ttcgaagaga
     8221 aactcaccac ccgggccttg caggagctgg tacaggggca cctgcggttc aagatggtgc
     8281 ggcggcggcg gtcggaaagc tgatgcgcct cgccggccgc cacgtcgtac tcggcgtgtc
     8341 gggcggtatc gcctgctaca agagctgcat cctcgcccgg cgtctggtcg aggacggcgc
     8401 gaccgtggac gtggtgatga cgcaggcggc gacggaattc gtgcgaccgc tgacgttcga
     8461 ggccctcacc ggccgcaagg tgatcaccag cctgtgggag ccgggccgcg ccctggcaca
     8521 cgtggatctc gccaagcatc ccgatctcgt catcgtggca cccgccacgg cgcagctgct
     8581 cgctcgggcg gcgcagggac tcgcggatga tttcctcacc tcactgctca tcgcccggag
     8641 cgcgcccatc ctcgccgccc cggcaatgaa cgacgggatg tacgcgcacc ccgcgaccca
     8701 ggccaatctc cagaccctcc gtgaccgcgg ctggcacttc gtcggccccg atgtcggcgc
     8761 gctggccgag ggtccgagtg agcggcccgg ccggatgagc gaaccggaag cgattgtgag
     8821 tgaggtgcgc cggatcctcg cgcccgcggg gccctggcgg ggccggcacg tcgtcgtgac
     8881 ggcgggcccg acccgcgagc acctcgatcc cgttcgcgtc atcaccaatc cgtcttcggg
     8941 acggatgggg tatgccttgg ccgaggccgc gttggcgcgc ggtgccgtcg tcaccctcgt
     9001 cgcgggaccg accgacctcc ctccacccag tggcgccacg gtggtgcgca tcgagtcgac
     9061 cgaagacctc cgccatgaga ttcagcgcgt cctcggcggc gcctcggcgc tgttcatggc
     9121 agcagcgccc gccgacttca agcccgcgaa ggcggacaag accaagcggg cgcgctccaa
     9181 gggcaagctg acgctcaccc tcgctccgac gcctgatgtg cttcagtctc tgaaaaggcc
     9241 caaggattgc ctgatggtgg gattcgcgct cgaaaccggt gacggcatga agcacgcgga
     9301 gcggaagctc aaggaaaaga aactcgacgc gatcgtcctg aacgacgcga cggagcccgg
     9361 ggcgggcttc gaagtgagca ccaacaaggt gaccatcatt acgcgggatg gcgacgtgca
     9421 ggcgcttccg ctgctctcca agcgcgaagt ggcggataaa atcctcgacg cggtggagtc
     9481 gaagctctcg tgaccgatgg tcaggacggc cgggacggcc ggg
//
LOCUS       DASUBP010000202         2872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_619020, whole genome shotgun sequence.
ACCESSION   DASUBP010000202 DASUBP010000000
VERSION     DASUBP010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619020"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..599
                     /locus_tag="VFU75_07665"
     CDS             <1..599
                     /locus_tag="VFU75_07665"
                     /inference="COORDINATES: protein motif:HMM:NF014799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5466753.1"
                     /translation="RAMPNLLVLRPADATETVEAWRVALRHPGGPVGLVLTRQKLPVL
                     DRSVLAPAAGTARGAYVLCDANPDAPPELILIATGSEVSLALAAHRQLSREGVRSRVV
                     SMPSWELFESQPAAYRDAVLPPGVRARVSIEAGSPFGWERYVGPTGAIIAVDHFGASA
                     PGPVVMAQYGFTVERVIAVARETLAVCSSGGRWGPTCT"
     gene            696..789
                     /locus_tag="VFU75_07670"
     tRNA            696..789
                     /locus_tag="VFU75_07670"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:730..732,aa:Sec,seq:tca)
     gene            937..1719
                     /gene="fabG"
                     /locus_tag="VFU75_07675"
     CDS             937..1719
                     /gene="fabG"
                     /locus_tag="VFU75_07675"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HEU5466754.1"
                     /translation="MEIALAGRRALVTGGNSGIGEAIAVALGASGARVAVNYVVHPEA
                     AEAVVARIRKAGGEALAVAADVADAGEVARMFGQVDQAWGGVDILVNNAGIDGGRALA
                     WEADPAQWAKVLAVNLGGAFHCAREALRRMVPAKAGVIVNLTSVHERIAWTGYSAYTA
                     SKAGLSMLTENLAQEAAPAGVRVVAVAPGAIKTPINRSVWSDPNTLADLLDKIPLNRM
                     GTPEEIARMVVVLASDVASYVTGTTVFVDGGMTDYPSFREGG"
     gene            1717..1808
                     /locus_tag="VFU75_07680"
     tRNA            1717..1808
                     /locus_tag="VFU75_07680"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1751..1753,aa:Sec,seq:tca)
     gene            1896..2471
                     /locus_tag="VFU75_07685"
     CDS             1896..2471
                     /locus_tag="VFU75_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466755.1"
                     /translation="MTIGIVMATTPGWLGAQAASPVGGAKAPPPTSSMDSLISAFSGR
                     WSLKLAFEPSKDMPQGLQGTGEETWHAGPQGVTLTDEEVFTLGPQTVIVVGLLWRDNK
                     THEFHALDCSNQNASTCDLKGAVADVVVHWTGSELTIDEKELSQGTVMTSRVTWSDIT
                     ANTFTETGYLAPPGGPFQKIMTIHATRATAN"
BASE COUNT          465 a          875 c         1026 g          506 t
ORIGIN      
        1 tgcgggcgat gccgaatctc ctcgtcttgc ggcccgccga cgccaccgaa accgtcgaag
       61 cgtggcgcgt cgcgctgcgt catcccgggg ggccggtggg cctggtgctc acccggcaga
      121 agctgccggt gctcgatcgc agcgtcctgg cgccggctgc cggcaccgcc cgtggcgcat
      181 acgtgctctg cgatgccaat ccggacgcgc cgccggagct gatcctgatc gcgaccggct
      241 cagaggtatc gcttgcactg gctgcgcatc ggcagctcag tagggaaggg gttcggagtc
      301 gggtagtgtc gatgccgtcg tgggagctgt tcgagtcgca gccggcggcc taccgcgatg
      361 ctgtgcttcc cccgggggtg cgagcccgcg tcagcatcga ggccgggtcg ccctttgggt
      421 gggagcgcta cgtcgggccc actggcgcca tcatcgcggt ggaccacttc ggcgcgtcag
      481 cgcccgggcc ggtcgtgatg gcgcagtatg ggttcaccgt cgagcgggtg atcgcggtgg
      541 cccgcgagac gcttgccgtc tgctcgtccg gtggtaggtg gggacctacg tgcacgtgat
      601 gtatcccgga tggtagcgag tgatgcgccg ttgagtcccg agtagtctgc tgccggactg
      661 gtcatgtggg tgtagtttgc agggcgacgt tgcctggggg cggatcgggt ctggtggtcc
      721 gactggtctt caaaaccagc tggagtccgc aaggactttg gtaggttcga ttcctacacg
      781 ctcccgccac gactcgcctt cgctgactct ctccgccccc acgtcaaacg gatccgtcac
      841 gcacggtata ggtgggatgg tgttgcagct cgcgtatcaa agaccaactc ccgttcccgg
      901 tgtagcaggt ggcgggacaa cttcgggagc gcgatgatgg agattgctct tgcgggacgc
      961 cgggcactcg tgaccggcgg gaattcagga atcggagagg cgatcgcggt ggccctcggc
     1021 gcgtcgggcg cgcgggtggc ggtgaattac gtggtgcatc cggaggccgc ggaggccgtg
     1081 gtcgcccgga ttcgcaaggc cgggggggag gcgctggcgg tggcggcgga tgtcgccgat
     1141 gccggcgagg tggcgcggat gttcgggcag gtggatcagg cctggggcgg cgtggacatc
     1201 ctggtcaaca acgccggcat cgacggtggg cgggccctcg cgtgggaggc ggacccggcg
     1261 cagtgggcga aggtcctggc ggtgaatctc ggcggcgcct ttcactgtgc gcgggaggca
     1321 ttgcggcgca tggtcccggc caaggcgggg gtcatcgtga atctgacgtc ggtgcacgag
     1381 cgcatcgcct ggaccggata cagcgcctac accgcgagca aggcggggct cagcatgctc
     1441 acggagaacc tggcgcagga ggccgcgccc gctggggtgc gggtggtggc ggtggcgccg
     1501 ggggccatca agacgccgat caatcggagt gtctggagcg acccgaacac gctggccgat
     1561 ctcctcgaca agattccctt gaaccggatg gggaccccgg aggagatcgc ccgcatggtg
     1621 gtggtactcg cctccgacgt ggcgagttat gtgactggga cgacggtgtt cgtggacggc
     1681 ggaatgacgg actatcccag ctttcgcgag ggtggatgag gcggatcggg tctggtgggc
     1741 cgactggtct tcaaaaccag ctggagtccg ctaggacttt ggggttcgat tccggtacac
     1801 tccagccaca acggctggca aggcactcaa ccccgcaatc tcacactcgg ccctcgcgcc
     1861 gatccaggag gaccagtatg cgctggatat ctgcagtgac gatcggcatc gtgatggcca
     1921 caaccccagg gtggttgggg gctcaggctg ccagtcccgt cggcggcgcg aaggcgccgc
     1981 caccgacgag ttcgatggac agtctgatca gcgcgttctc ggggcggtgg tcgttgaagc
     2041 tcgccttcga accgtcgaag gacatgcctc aagggctcca gggtaccggc gaggaaacct
     2101 ggcacgcggg cccgcagggc gtgaccctca ccgatgaaga ggttttcacc ctgggtccgc
     2161 agaccgtcat cgtcgtcggt cttctctggc gggacaataa gacccacgag tttcacgcgt
     2221 tggactgcag caaccagaat gccagtacgt gtgacctcaa gggcgcggtc gccgatgtgg
     2281 tggtgcattg gaccggcagc gagttgacga tcgacgaaaa ggagctctca cagggtacgg
     2341 tgatgacgtc gcgcgtcacc tggtccgaca tcaccgcgaa cacatttacg gagaccggat
     2401 acctcgctcc tccggggggc ccattccaga agatcatgac cattcacgcg acccgcgcaa
     2461 cggccaacta gtcgatgcgt cgccggatgt ctctagcgca ctgatgtcag ggggagttat
     2521 gcgtcctgta catggtctcg ccatcctcgt tgccgccgcc gcatgcaccc agccagaatc
     2581 gccaagccgg ggcctgactg gcacatggac actggagcgg gggcaacgcg cgctggagcc
     2641 gcaggcgctg acgctgtatc aggttggcgg tgcaatatcg gggaccgcca ccatcccagg
     2701 cctggacccc gtgagtccag ggcaacccgt tgtctcggtc agcgggtcct acgcgtcacc
     2761 aaccgccacg atggacatcc ggatcgggac ttccatcgcc ggtcactacg ccgcaacgct
     2821 ggattctgcc aatcatctga cgggagtctt caccttcagc actgcgctcg gc
//
LOCUS       DASUBP010000203         4403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_237185, whole genome shotgun sequence.
ACCESSION   DASUBP010000203 DASUBP010000000
VERSION     DASUBP010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4403
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_237185"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VFU75_07690"
     CDS             complement(<1..678)
                     /locus_tag="VFU75_07690"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466756.1"
                     /translation="MSTPFHPFALDRRYRLVRLVVLLVFAVLIVAFFRTQILGYSRYR
                     LQSETNRLKEIVLPAPRGLILDRNGKVLADNIPGYTISLLPDDTAKLHSRLRQIAPFA
                     DLDSEAIARVLDRFLRAPYQPALVMANAPADLVAQLEERRINIPGLYIQAEPRRYYPD
                     SGAAAHLLGTVGEVTEDERNSPRYAGLRLGALVGKTGLEREYDDSLRGQDGQRFVEVS
                     AVGRVVRE"
     gene            complement(675..1172)
                     /gene="mreD"
                     /locus_tag="VFU75_07695"
     CDS             complement(675..1172)
                     /gene="mreD"
                     /locus_tag="VFU75_07695"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEU5466757.1"
                     /translation="MRRTDKYRFWFVLAGLVLLHFSVRTRLGDDRIAPDFLLLALLMY
                     TIRAAPGPSAGAGFIVGIIRDALTPASFGASALAHTLVGYISSWSKAVFFAENLFVNG
                     CIFFGGTWIRNLVVSLASGELKGQTLVWELLVWSPLQSLMTALVGVLVLIVLGRWLSV
                     RFTDA"
     gene            complement(1169..2023)
                     /gene="mreC"
                     /locus_tag="VFU75_07700"
     CDS             complement(1169..2023)
                     /gene="mreC"
                     /locus_tag="VFU75_07700"
                     /inference="COORDINATES: protein motif:HMM:NF016011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEU5466758.1"
                     /translation="MTIEAERYGSRADTFVFLGCLVLSIIAMTVPDSIRRPFSQALRQ
                     TILAPLLALQEETATLKTSRAHVDEITAQRDSAMLAATFLPELRSENARLRSLLGLGQ
                     RLATGYVPAEVLHESGPTDPLTFLVSAGRKDGVKTLDPVVSPGGLVGVVSAVDEQTSV
                     VATWAHPEFRAAAMALDGSIYGIVAPHGTEGPGIWLLELQGVPYRSVVPESTLIVSSG
                     LGGVIPRGIPIGTVIGPGADNEHWERTYLLRPAVHPAAVTHVMILTGTTKGSGNLHDL
                     FQDEGKSP"
     gene            complement(2031..3074)
                     /locus_tag="VFU75_07705"
     CDS             complement(2031..3074)
                     /locus_tag="VFU75_07705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HEU5466759.1"
                     /translation="MPFSWKNLKFGAWSPANEIAVDLGTANTLVYVKGEGIVLNEPSV
                     VAIEKATGKVKGIGLEAKRMLGRTPDGILAVRPLKDGVIADFDVTEKMLRFFLKTIID
                     RHLFRVKPKVIVCVPSGITEVEKRAVRDSAQSAGAKEVYMVAEPMAAAIGVGLPVETP
                     TGNMVIDIGGGTTEIAVIALSGIVSDTSIRTGGDELDQAIVQFMRKNYNLLIGEPTAE
                     QIKIQIGSAAPVGEEREMEVKGRDLVSGIPKIVRVHSSEVREAVQEPIQQIVDAVRRA
                     LEITPPELASDIVDRGIVMTGGGALIRGLDVLLQQETGLPIHLDEDPMTCVVRGVGRI
                     LDDPEKYRNVLTT"
     gene            complement(3175..>4403)
                     /locus_tag="VFU75_07710"
     CDS             complement(3175..>4403)
                     /locus_tag="VFU75_07710"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU5466760.1"
                     /translation="SATVAGRVVVFSVPDSIKGFRSMFGAFQPILGHSGIAQSAGIVM
                     AGYDRLPPDLIPQILAGRVTTDTAPVQTKVAPRFLITPRSLEALFGSRPKVGAVGAPV
                     SGSLRWLRQPLDHPARNIIAKLPGSDPALRGEYVSMSAHHDHVGFTGLPVDHDSVYAY
                     NRVVRPMGADSPQRPATPAEETRVKALRDSLQKAAGDRPDSIFNGADDDGSGTVGLMA
                     VARSLAAAPHPRRSILIVSHAAEERGLLGSAWFTDHPTVPRDSIVGEIDMDMVGRGNA
                     SDLPKGGPGYLEVVGAKRLSTQFGELFEKVNSGEAQPFTFNYEFDVPGHPLQYYCRAD
                     HYSYARYGIPATAISRGEHADYHQVTDEAEYIDYTVLARVATLARDFAVAVANLDHRP
                     VVDGPKQSDPHGPCRQ"
BASE COUNT          783 a         1498 c         1413 g          709 t
ORIGIN      
        1 ctcccgcacg acccttccca ccgcactcac ctcgacgaac cgctgcccgt cctgtccccg
       61 caaggagtcg tcgtactcgc gctcgagacc cgtcttcccc accagggcgc ccagcctgag
      121 gcccgcatac cgcggcgagt tgcgttcgtc ctccgtgacc tcgcccaccg ttcccaggag
      181 atgcgcggcc gcccccgagt ccgggtaata ccgccgcggc tcggcctgaa tgtacagccc
      241 gggaatgttg atccggcgct cctcgagctg ggccacgaga tccgccggcg cgttggccat
      301 caccagcgcg ggctgatacg gcgctcgcag gaaccggtcg agcacgcgtg cgatcgcctc
      361 cgagtcgaga tccgcgaacg gcgcgatctg ccgcagccgg ctgtgcagct tcgcggtgtc
      421 gtccggcaac agcgagatcg tgtatccggg gatgttgtcg gccagcacct tgccgttccg
      481 gtcgaggatc aacccgcgcg gtgccggaag aacgatttcc ttgaggcggt tggtttcgga
      541 ctgaaggcgg taccggctgt atccgaggat ttgtgtgcgg aagaaggcga cgatcaggac
      601 cgcgaagacg aggagcacca ccaaccgcac cagccggtag cggcggtcga gcgcgaacgg
      661 atgaaacggc gtgctcatgc gtcagtgaac cggaccgaca accaccggcc cagtacgatc
      721 agcaccagca cgcctaccag ggcggtcatc aggctttgca gcggagacca caccaggagc
      781 tcccacacca gcgtttgacc cttcagctcg ccggaggcaa gggacaccac gaggttccgg
      841 atccaggtgc cgccgaagaa gatgcagcca ttcacgaaca gattctcggc gaagaacacc
      901 gccttggacc aggacgagat gtagcccacc agcgtgtgcg ccagcgcgct cgcgccgaaa
      961 gacgcgggcg tgagcgcatc acggatgatc ccgacgatga acccggcccc cgccgacggt
     1021 cccggagccg cgcggatcgt gtacatcaag agcgcgagca ggagaaaatc gggtgcgatg
     1081 cggtcgtcac ccagccgggt ccgcaccgag aagtgcagca gcacgagccc ggcgagcacg
     1141 aaccagaacc ggtacttgtc ggttcgcctc atggactctt gccttcgtcc tgaaagaggt
     1201 cgtggagatt gccgctcccc ttggtcgttc ccgtcagaat catcacgtgc gtcacggccg
     1261 cggggtgcac cgccgggcgc aggaggtagg tccgctccca gtgctcgtta tccgcgcccg
     1321 gcccgatcac cgtgccgatc gggatgccgc gcggaatcac ccccccgagg cccgacgaga
     1381 cgatgagcgt actctccggc accaccgaac ggtacggaac cccttgcagc tcgagcagcc
     1441 agatccccgg cccctccgtg ccgtgcggcg cgacgatgcc gtagatgctg ccatccagcg
     1501 ccatcgccgc cgcgcgaaac tccgggtgcg cccaggtggc cacgacgctc gtctgctcat
     1561 ccaccgccga caccacgccg accagcccgc cggggctgac gaccggatcc agcgtcttca
     1621 ccccatcctt ccgcccggcg gacacgagaa acgtcagggg atccgtggga cccgattcgt
     1681 ggaggacttc cgccgggacg tacccggtgg cgaggcgctg gccgagcccc agcaggctgc
     1741 ggagacgggc attctcggag cggagctcgg gcaggaacgt cgccgcgagc atggcggaat
     1801 cgcgttgcgc cgtgatctca tcgacgtggg cccgggaggt cttcagtgtt gcggtttctt
     1861 cctgcagggc gagcagtgga gcgagaatcg tctgacgtag ggcctggctg aacggacgcc
     1921 ggatggaatc cggcacggtc atcgcgatga tggagaggac gaggcacccg aggaagacga
     1981 acgtgtccgc ccgcgatcca tagcgttcgg cctcgatggt catccccgcc tcaagtggtc
     2041 aggacgttgc ggtatttctc cggatcgtcc aggatccggc cgaccccgcg taccacacag
     2101 gtcatcggat cttcgtccag atgaatgggc agtccggttt cctgctgcag gaggacgtcg
     2161 agaccgcgga tgagcgcccc gccgcccgtc atgacgatgc cgcggtccac gatgtcggag
     2221 gcgagctcgg ggggcgtgat ttccagcgcg cgccgtaccg catccacgat ctgctggatc
     2281 ggctcctgta ccgcctcgcg cacttcgctc gaatgcacgc gcacgatctt cggaatgccc
     2341 gagaccaggt cgcgtccctt cacttccatt tcccgctctt cacccaccgg ggccgccgag
     2401 ccgatctgga tcttgatctg ctccgcggtg ggctcaccga tcagcaggtt gtagttcttg
     2461 cgcatgaatt ggacgatggc ctgatccagc tcatcgccgc ccgtccggat ggacgtatcc
     2521 gacacgatgc ccgagagcgc gatgaccgcg atttccgtcg tcccgccgcc gatgtcgatc
     2581 accatgttcc cggtaggcgt ctcgacgggg aggccgaccc cgatggcggc cgccatcggc
     2641 tccgccacca tgtagacctc tttcgcaccc gccgattgcg cggagtcgcg caccgcgcgc
     2701 ttctccactt cggtgatccc ggaggggacg cacacgatca ccttcggctt cacgcggaag
     2761 agatgacggt cgatgatggt cttgaggaaa aaacggagca tcttctcggt gacgtcgaag
     2821 tcggcgatga cgccgtcctt cagcggacgc accgcgagaa tgccgtcggg cgtgcgcccg
     2881 agcatgcgct tcgcttcgag tccgatcccc ttgaccttgc cggtcgcttt ttcgatcgcg
     2941 accaccgagg gctcgttcaa gacgatgcct tcgcccttca cgtatacaag cgtgttcgcc
     3001 gtgccgagat cgacggcgat ctcgttcgcg gggctccagg caccaaattt cagattcttc
     3061 cagctaaatg gcacaggcag tcaggggcaa aggaaaaggg gggcgctagc tcaagcgccc
     3121 ccaaggtaac aaatgcccta actgcaagca atactgatgt ttgacgaagt ccccttattg
     3181 acggcaggga ccgtgcggat cgctctgctt gggtccgtcc accaccggcc gatggtcgag
     3241 gttggcgacc gccaccgcga agtcccgggc gagggtcgct acccgtgcca gcacggtgta
     3301 gtcgatgtac tccgcctcgt ccgtcacctg atggtagtcg gcgtgctccc cccgcgagat
     3361 cgcggtggcc gggatcccgt accgtgcata gctgtagtgg tcggcccggc agtagtactg
     3421 gagcggatgc cccgggacgt cgaactcata gttgaacgtg aagggctgag cctcccccga
     3481 gtttaccttc tcgaacagct ccccaaactg cgtcgacaac cgcttggcgc cgacgacctc
     3541 caggtaaccg gggccaccct tcggcaaatc gctcgcattg ccgcgcccca ccatgtccat
     3601 gtcgatctca ccgacgatcg agtccctggg caccgtcggg tgatcggtga accacgccga
     3661 ccccaagaga ccgcgctctt ccgcggcatg ggagacgatg aggatggacc ggcgcggatg
     3721 cggagccgcc gccagcgagc gggcgaccgc catgagtccc acggtgcccg agccatcatc
     3781 gtccgcgcca ttgaagatcg aatccgggcg atcccccgcc gccttctgga gcgagtcccg
     3841 gagggccttc acgcgggtct cctcagccgg ggtcgccgga cgctggggcg aatcggcccc
     3901 catggggcgc accacccggt tgtaggcgta caccgaatca tggtccaccg gcaggccggt
     3961 gaacccgacg tggtcgtggt gtgcgctcat ggacacgtac tcgcccctca gggcgggatc
     4021 cgatcccggg agcttggcga tgatgttgcg ggccggatgg tcgaggggct gccggagcca
     4081 gcggagcgaa ccggacaccg gggcgcccac ggcgccgacc ttcggcctgg aaccaaagag
     4141 cgcctcgaga gaccgcggcg tgatcaggaa ccggggtgcg accttggtct ggaccggagc
     4201 cgtgtccgtc gtgacccggc ccgccagaat ctggggaatg aggtcgggtg gcaggcgatc
     4261 gtaacccgcc atcacgatgc cagccgactg ggcgatcccc gagtgtccca agatcggttg
     4321 gaatgccccg aacatcgagc ggaagccctt gatcgaatcg gggaccgaga acaccacgac
     4381 ccgcccggcc accgtcgcgg aat
//
LOCUS       DASUBP010000204         3392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_482765, whole genome shotgun sequence.
ACCESSION   DASUBP010000204 DASUBP010000000
VERSION     DASUBP010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482765"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..253
                     /locus_tag="VFU75_07715"
     CDS             <1..253
                     /locus_tag="VFU75_07715"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5466761.1"
                     /translation="DHRPSPEEILDDEARRRRVREALDQLTPRDREVLLLWDAGLSYD
                     EIAAQTGLARTAVGTTLARARRRLVEAYGSGSEDVRVAR"
     gene            240..1226
                     /locus_tag="VFU75_07720"
     CDS             240..1226
                     /locus_tag="VFU75_07720"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HEU5466762.1"
                     /translation="MSHVDEGTLHAYLDGELPDAERARFEAHLTECAACRGRLAEERG
                     LIERADRLLTLAELPTGHTHRPPPASRVQSPRRQPRFVVAAAWAASIALAFVTGWLLR
                     PTPQRAGNAADETKQVAAVVPADSAASAAHQDAPAPATLYRARASHLQATKTDRESLA
                     VAAETSVVPMVKPGVVGNAGRPALRGAAPAPIPPATVAITPSTAAPRTAATADMAVGT
                     AERRAIGTTWTAIAAEPARRALGTDVARIPGIPVRDILQNPKVPNEVMVEQEVADGTI
                     IQLLQSPLDSAVEAMGYGYSRNPLQKAVGRLQVRISGPLAPDSLLKLLDRVR"
     gene            complement(1201..2175)
                     /locus_tag="VFU75_07725"
     CDS             complement(1201..2175)
                     /locus_tag="VFU75_07725"
                     /inference="COORDINATES: protein motif:HMM:NF024869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HEU5466763.1"
                     /translation="MNLWAKACEMARAGAGGALATIADTSGSTPVPAGVKMLIGEADR
                     LAGSIGGGCVEGDVIQVAIDAQRSGHPALVTHHLNADLAGDVGLSCGGTVRIFVEPLS
                     TDPRYVEALAAAATATLGQVTTAVDWSNGPHKTFGPLPGGTSPVADAHISDDERFVVE
                     RLVQAPRLLVFGAGHVGAAIARAAGAAGFRVTITDDRPEFADAARFPGLDVHALPPDR
                     VLSEFAPGPADAVVVATRGHRNDAEILDQLARMTVGYVGLLGSARKRAVILKALGAAG
                     APADALAAVRVPVGLEIGAVTPEEIAVSVTAELIAWRRLPSTGPGRAA"
     gene            complement(2172..2516)
                     /locus_tag="VFU75_07730"
     CDS             complement(2172..2516)
                     /locus_tag="VFU75_07730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU5466764.1"
                     /translation="MTDSLESFRHFREKMNAAILAAGNTTINRFFALDTRTYEAGALP
                     VKTKELLGLVASMVLRCDDCVTYHVIRCREEGVSDAEFQDAFAVALVVGGSIVIPHLR
                     RAVARLEELPPA"
     gene            complement(2555..2905)
                     /locus_tag="VFU75_07735"
     CDS             complement(2555..2905)
                     /locus_tag="VFU75_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466765.1"
                     /translation="MTIRLGAALALAGALVSCGKSATAGGAAHSLTVSVRDNMFSPKV
                     DSISVGDTVTFTWQGSQQHDLVFQDTTVGNVSSQSSGTAKRGFPLPGIYLYRCTLHSS
                     DFNTGSMIGTIAVY"
     gene            complement(2902..3291)
                     /locus_tag="VFU75_07740"
     CDS             complement(2902..3291)
                     /locus_tag="VFU75_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466766.1"
                     /translation="MNKILFGLLGAGVVVGVACSNPTGNGSGCGGNGGNVVTITAHDN
                     QTFTPQTVQVSQTQQVCFQNLGTVLHTVTPDSEMPGDTLWIRGHPTEALPPNSPYQFT
                     LGVGDYYYHCAYHGGNQTGMWGVIHVR"
BASE COUNT          567 a         1187 c         1125 g          513 t
ORIGIN      
        1 ggaccatcga ccgtcacctg aagagatcct cgatgacgag gcacgccggc gccgggtgcg
       61 tgaagccctg gatcagctga ccccgcggga ccgcgaggtg ctgcttctct gggatgccgg
      121 actctcgtac gacgagatcg cggcgcagac cgggctcgcg cgcaccgctg tcggcaccac
      181 gctggcgcgc gcgcgccgcc gtctggtcga agcctatgga tcgggaagtg aggacgtccg
      241 tgtcgcacgt tgacgaagga acccttcacg cctatttgga cggcgagctg cccgacgcgg
      301 agcgcgcccg gttcgaggcg catctgaccg agtgtgctgc ctgccggggt cgcctggcgg
      361 aggagcgcgg actcatcgag cgcgccgacc ggctcctcac gctcgctgag ctccccaccg
      421 gccacacgca ccgccccccc ccggcgtccc gcgtgcaatc gccgcgccgg cagccccgat
      481 ttgtggtagc ggccgcgtgg gccgcatcca tcgcgctggc gttcgtcacc ggatggctgc
      541 tgcggccgac gccccagagg gccggaaatg cggcagatga gacgaaacag gtcgcggccg
      601 tggttcccgc cgattcggcg gcctccgcgg cacaccagga cgctcccgct ccggcgactc
      661 tgtatcgcgc gcgcgcctcc catcttcagg cgacgaagac cgatcgggag tcgcttgccg
      721 tcgcggcaga aacgagcgtc gtccccatgg tgaagcctgg ggtggtcggg aacgccggac
      781 gccccgccct ccggggcgcg gcccccgcac ccatcccccc tgccaccgtc gccatcacgc
      841 cgtccacggc agcgccacgg actgcggcca ccgcggacat ggcggtgggg acggctgaac
      901 gccgcgccat cggaacgacg tggaccgcga ttgccgccga gccagcgcgg agggctctgg
      961 gcaccgacgt cgcccgtatc ccggggattc cagtgcgcga cattctccag aaccccaagg
     1021 tgccgaacga agtgatggtg gagcaggaag tagcggatgg aaccatcatc cagctgttgc
     1081 agtcgcccct ggactcggcc gtggaggcca tgggctacgg ttactcgcga aatccactcc
     1141 agaaagcggt ggggcgtctg caggttcgga tctccggacc tctcgcgccc gactccctgc
     1201 tcaagctgct cgaccgggtc cggtagaggg caatcgccgc caggcgatga gctccgcggt
     1261 gacgctgacc gcgatctcct cgggcgtcac cgctccgatc tccagtccca ccggcacccg
     1321 gaccgccgcc agggcatcag ccggcgcgcc ggcggcgccg agggctttca ggatcaccgc
     1381 gcgcttccgt gcgctgccca gcaaccccac atacccgacc gtcatccgcg ccagctggtc
     1441 caggatctcc gcgtcgttcc ggtggccgcg cgtcgcgacc acgacggcgt cggcgggtcc
     1501 cggcgcgaac tcgctcaata cgcggtccgg aggcagggcg tggacgtcca ggcccggaaa
     1561 acgggcggca tcggcgaatt cgggacgatc gtcggtgatc gtcacccgga acccggcggc
     1621 acccgccgcg cgggcaatgg cggcaccgac gtgcccggct ccgaacacta ggagtcgagg
     1681 cgcctgaacc aggcgctcga ccacaaagcg ttcgtcatcc gagatgtggg cgtccgcgac
     1741 gggagatgtc ccgcccggca ggggtccgaa tgtcttgtgc ggcccgttcg accagtcgac
     1801 ggcggtggtc acctggccca gcgtggcggt ggccgcggcc gcgagcgcct ccacgtacct
     1861 cgggtccgtc gagagcggtt cgacgaagat ccgcacggta ccgccacagg agagccccac
     1921 gtccccggca aggtcggcgt tcaggtggtg cgtgaccagc gccggatgcc cggatcgttg
     1981 cgcgtcgatc gccacctgga tgacatcccc ctccacacag ccaccgccga tcgagcctgc
     2041 caggcgatcc gcctccccga tcagcatctt cacgccggcc ggcacgggcg tggaccccga
     2101 ggtgtccgcg atcgtggcca acgccccccc cgcgccggcc cgggccatct cgcacgcctt
     2161 ggcccagaga ttcacgcggg cgggagctct tccagccggg cgacggcgcg gcggagatgc
     2221 gggatcacga tggaccctcc caccaccagc gcgacggcga acgcatcctg gaactcggcg
     2281 tcactgaccc cttcttcccg gcaccggatg acgtggtagg tcacgcagtc gtcgcagcgc
     2341 aggaccatcg acgccaccag ccccaggagc tccttggtct tgaccggaag cgccccggct
     2401 tcataggtcc gggtgtcgag ggcgaagaag cggttgatgg tggtgttgcc ggcggccagg
     2461 atggccgcgt tcatcttttc gcggaaatgc cggaacgatt cgaggctgtc ggtcatacga
     2521 caaacctaga cggctagacg ggtagacggc taggtcaata aaccgcgatg gtgccgatca
     2581 tggatcccgt gttgaagtcg gacgaatgga gggtgcagcg gtagaggtag atccccggca
     2641 gcgggaaccc acgtttggcg gtcccgctag actgcgatga tacgttgccg acggtggtgt
     2701 cctggaagac cagatcatgc tgctgactgc cctgccaggt gaaggtcacg gtgtctccca
     2761 cgctgatact gtccaccttg ggggagaaca tgttgtcgcg cacactcacc gtgagcgagt
     2821 gggcggcacc accggcggtc gcgctcttgc cgcacgacac gagggctccc gccagcgcca
     2881 gcgccgcacc cagacggatg gtcatcggac gtgaatcact ccccacatgc cggtctggtt
     2941 cccgccatga tacgcgcagt gatagtaata gtcaccgact ccgagcgtga attgatacgg
     3001 agagttgggc ggcaaggcct ccgtgggatg gccgcgaatc cacagcgtgt cgcccggcat
     3061 ctccgagtct ggagtcaccg tatggagcac agtgcccaga ttctggaagc agacttgctg
     3121 cgtctgactg acctgcaccg tctgaggagt gaaggtctgg ttgtcgtggg cagtgatcgt
     3181 cacgacgttc ccgccattcc caccgcagcc gctcccattg ccggtcggat tcgagcaggc
     3241 cacgccaacc accacgccgg ctcccagcag cccgaacaag attttgttca tgggtccccc
     3301 cgcgaagagt caaacatgga atccctcttg atacgcctga aactacaggg ggctccggcc
     3361 tgagccagag cccccatgtg ccggatcgga ac
//
LOCUS       DASUBP010000205        34854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_32599, whole genome shotgun sequence.
ACCESSION   DASUBP010000205 DASUBP010000000
VERSION     DASUBP010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 34854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34854
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32599"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="VFU75_07745"
     CDS             complement(<1..224)
                     /locus_tag="VFU75_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466767.1"
                     /translation="MTISSEISDLLEFTEAQAFADMLAAAPREWGAWSERRAGGWILQ
                     VPTIDILLFNRVIGAGLPAATREEIDEVIQP"
     gene            complement(221..604)
                     /locus_tag="VFU75_07750"
     CDS             complement(221..604)
                     /locus_tag="VFU75_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEU5466768.1"
                     /translation="MTLKVVATDQAPGAIGPYSQAIVANGFVYTAGQVGFDPKKMELV
                     GTTVGEQTEQVMKNLAAVLKAAGSGLDRVVKTTVFLADMADFQAMNEVYAKHFGNHKP
                     ARSTVAAAGLPRNARVEIEVVATTG"
     gene            complement(606..2279)
                     /locus_tag="VFU75_07755"
     CDS             complement(606..2279)
                     /locus_tag="VFU75_07755"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEU5466769.1"
                     /translation="MSTVPSDIAIAQAAKMRPVVDIARELGLADDEVELYGKYKAKIQ
                     LSAISKRKPKGKLILVTGINPTPAGEGKSTVTVGLTQALRKIGKDAILCIREPSLGPV
                     FGVKGGAAGGGYSQVVPMEDINLHFTGDFHAISSAHNLLSAMLDSHLHHGNASGLDTR
                     RITWPRTIDMNDRALRSIIVGLGGINGGPAREERFVIIPGSEVMAIMALATSIEDLEA
                     RLARIIVGLTANKTAVRASDLKAQGAMTLLLKDALKPNLVQTLEGGPALVHCGPFGNI
                     AHGCNSVMATQLGLRLADLVLTEAGFGADLGAEKFLDIKCRMAGLKPDAAVVVATVRA
                     LKMHGGVKKDALGPENVAAVEKGVVNLQRHVQNVKKYGLPVVVALNRFITDTDAEIAA
                     VKKACAAEGVEVVRCEVWEKGGAGGEEVARALLKLLDTEKSNYHPLYDEKKPIREKIE
                     TIAKEIYGAGAVAFAPAAERAMELLPKLGLGETPVCMAKTQYSFSDDATKLGAPSGFT
                     LQVRDILPSAGAGFVVALAGDIMTMPGLSKEPAAQKIRMHPDGTIEGLF"
     gene            complement(2294..3073)
                     /locus_tag="VFU75_07760"
     CDS             complement(2294..3073)
                     /locus_tag="VFU75_07760"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEU5466770.1"
                     /translation="MLVDAHCHLGDGAFDGDRDDVRARARAAGVGHVVVIGDRVGTSR
                     DALALARSASGLSATAGIHPHQATEWSAATRAELLGLLAEPEVVAVGETGLDYHYDHS
                     PRPQQIESFEGQLALAESVRKPVVVHARDADADVARLLRSWGARVPAIILHSFSSGPD
                     LFAAGMEIGAYFSFSGMITFKSWGHPEYLTGCPADRLLVETDAPYLAPVPHRGSRNEP
                     AFVRETALALARARGVTLEALVPMLTANATRVFGARVTPSP"
     gene            complement(3075..4115)
                     /gene="secF"
                     /locus_tag="VFU75_07765"
     CDS             complement(3075..4115)
                     /gene="secF"
                     /locus_tag="VFU75_07765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HEU5466771.1"
                     /translation="MFRLFANARYDFIKWRKWAYGLSALLIVPGFVWLLISPPNYSIE
                     FTGGTQVQIATLDHAIGAAQIRAALDAGGLHGAEISSYGADTAFMIRARLGETNSGTQ
                     QASDSTMRAVRSSFDRGLGPTKYKVMKYEGIDGKVGKELQGKAFLAIFFSFFVTLAYL
                     AIRFEWRFGTAAVLATAHDILTTIAFIKYMNLEISLVVVGAVLTMVGYSLNDTIIIFD
                     RVRENLRKFRRQNLYDVVNLSINETLPRSILTHGTTFATTTALALLGGEVIRPFALVM
                     AFAIFTGTFSSIYIAAPILMYIEKRWPGEDARGARILTSPMAMPAPGAAAAPATQPKA
                     AKAAPPTPSPAR"
     gene            complement(4115..5746)
                     /gene="secD"
                     /locus_tag="VFU75_07770"
     CDS             complement(4115..5746)
                     /gene="secD"
                     /locus_tag="VFU75_07770"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HEU5466772.1"
                     /translation="MKTIRNRLILIGALFLVAVYAIVPRSTQVKQLDPQSGQMVVVTE
                     RLVPITLGLDLQGGVHLDLEVDQSQGPVPDCKDAIERSEKVVRTRLNEFGTLEPVVQT
                     TGNCRLNVELPGESDPERAKRIVQQQAHLEFRITDMKNLFRDALPGIDKALHDAGVRG
                     TGNAGPSNTVSQLFGADSSKKKGAKAAPDTTNNSAVFSSVLFQGQLPGEYLVPVEQTH
                     LVDSLRKMTAFQTAMPRGIDLFWSTDSVVRGAREYKYLYAVESKAMLTGDELEKATAR
                     RDPVNNHSVVQFDLSRRGGRLFADATGRHVRDYMAIILDTLVQGQPPIINDQIGAHGQ
                     IDLQSKPLADAQDLALVLRAGALPVRLAIVDEQSIGPTLGADAIHDGIRAGAVGVALV
                     ILIMGIYYRLSGLLAVAALALYVLFTLAGLAIFGFTLTLPGLAGLALSVGIAVDANVL
                     IFERIREELQHGKSVRLAIDEGFKHAMSAIVDSNVSTALTAAILYLVGTGPVQGFAIT
                     LIVGIAASMITAIFVVRTFYMIWLQRRPDMTTVSI"
     gene            complement(5743..6651)
                     /locus_tag="VFU75_07775"
     CDS             complement(5743..6651)
                     /locus_tag="VFU75_07775"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004160.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEU5466773.1"
                     /translation="MSGTAGTVVTVGSFDGVHRGHQAVLAEIARRAKPSGRKSLLVTF
                     DPHPLEVVNPEAAPKLLTTPDEKREVLAASPVDEVVVLPFTRELSQLSATEFVGTVLR
                     ERYGMVDLVLGHDHGFGRGRSGDVETVRRMGLEQGFTVDVVDAVRDDGQPISSTVIRT
                     AVAHGELDRAARWLGRPHAFSGVVERGAGRGRTIGIPTINLTPPARKLLPPDGVYAVH
                     VWVGGRRYGGMMNQGARPTFGVTARGLETHVFDFAGDLYGERVRVEWVRRLRDVRTFP
                     SRDALIAQLEHDRAAARESLNLSGTR"
     gene            complement(6648..7490)
                     /gene="truB"
                     /locus_tag="VFU75_07780"
     CDS             complement(6648..7490)
                     /gene="truB"
                     /locus_tag="VFU75_07780"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEU5466774.1"
                     /translation="MCLVAKPAGPTSHDIVDIARRALHQKRVGHLGTLDPFAEGLLVI
                     VVGRATRLAPYAAGWKKSYEGVIRLGSVTTTDDRTGEVTTASDAWRGLDLEQIRAASA
                     GLTGEIEQQPPAFSAVKVGGVRAYKKARRGEAVVVEARRVTVFEFTLSGWKAPDVMFR
                     ATVSGGTYLRSLARDLGERLGCGGHLAELRRTVVGPFRLEDAVPAQALTPADIKDPVL
                     LVRDLPSRTLDAAEREAIVHGRPVPARVADVGTVTLTADGCLLAVGEVVDGSIKPRLV
                     LADA"
     gene            complement(7499..7870)
                     /gene="rbfA"
                     /locus_tag="VFU75_07785"
     CDS             complement(7499..7870)
                     /gene="rbfA"
                     /locus_tag="VFU75_07785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEU5466775.1"
                     /translation="MMKRRSHRPERVAELVRQTVGAFLTGDVRDPRIGFVTVTGVEVT
                     ADLSHANVQVSVMGTEEEKTRSLEGLASAARFLRAQLSKELTLRTSPELHFRLDRGLE
                     HAQRINQVLKDLRSGSEEGES"
     gene            complement(7870..8151)
                     /locus_tag="VFU75_07790"
     CDS             complement(7870..8151)
                     /locus_tag="VFU75_07790"
                     /inference="COORDINATES: protein motif:HMM:NF016351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HEU5466776.1"
                     /translation="MIVGIMTWELHLDACHSLKDKRQVLESLKDRLHRKYNISVAETA
                     HQDLWQRAELSACVMSTDRVHAEKVLREADRLVDETAGARIVDTSTSYL"
     gene            complement(8152..10836)
                     /gene="infB"
                     /locus_tag="VFU75_07795"
     CDS             complement(8152..10836)
                     /gene="infB"
                     /locus_tag="VFU75_07795"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEU5466777.1"
                     /translation="MGTTRIHDLAAEFGISAEQLMGMLKEMDVFVRSHLSPLKDEQVA
                     LVRARWERDKRKSKEPAAPTKRRRAVKVAPEPVPEPVEARPKRRRRTAAEVAAAEAEA
                     SAAAEVEALAEQRARDALEAAAKAQIEEKPVVSLEERAAQLFKDLPRAADPLQPAAAS
                     AVVEPPLAPPAPSAPSAPPPPRPFIPTPVRPKPVASHTPGTPMPPRPVASGMPNFGGP
                     GAGGPGGGGGPGLDDRRRDKGGKKRKKGKKSVDQEAVAQNISRTLSALKGPVGRKGPH
                     RREEGPSYRDIQEQQRQEQKEREKTLVRVTEFITVSELASTLKVPPTQIVTFAFKELG
                     QMVTINQRLDFDMIELIASAFGFQAVQEEEYVSSGAPEEAADAAETLKPRPPIVTVMG
                     HVDHGKTSLLDYIRQANVVAGEAGGITQHIGAYHVTLKDGRTITFLDTPGHEAFTAMR
                     ARGAQVTDIVVLVVAADDAIMPQTIEAISHAKNAGVPLVVAINKIDLPSANVQKVKQD
                     LLAHGVVLEEFGGTTLMTPISAKKGTGVDKLLDQILLQAELLDLKANPDRRAAGAVLE
                     ATLDPGKGPVATILVQNGTLRVGDDFICGQFSGRVRAVTDERGQTVKDAGPSIPVQIL
                     GFEGVPEAGDTFTVMEDAGAAREIAQKRQRLEREAQHRRTGRVKTLEDFMAERVDGGA
                     ASLKIIIKADQGGPAEALADALAQLSTSEVKVEVVHRGVGAITDSDVLLAKASNAIII
                     GFHVRPDSNARASAERERVEIRTYRIIYEAVDEVKSAMEGLLAPEKKEVVLGEAEVRQ
                     VFKIAKVGTIAGSFVRSGVIPRTAKVRVIRNGVEVYDGTLASLKRFKDDAREVREGFE
                     CGIGVENFNDVKVGDLIEAYRIDEVARTLSGGTAAKAT"
     gene            complement(10838..11146)
                     /locus_tag="VFU75_07800"
     CDS             complement(10838..11146)
                     /locus_tag="VFU75_07800"
                     /inference="COORDINATES: protein motif:HMM:NF013417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HEU5466778.1"
                     /translation="MSDPARLVRLLGLGVRARTVVVGVSGTRAELHRGGVRCVVLASD
                     AGERTRDKVERLARATNVPVIVGPDAKALGAGLGRPPVQAIGVNDPSLARGVLGLSEE
                     "
     gene            complement(11143..12492)
                     /gene="nusA"
                     /locus_tag="VFU75_07805"
     CDS             complement(11143..12492)
                     /gene="nusA"
                     /locus_tag="VFU75_07805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HEU5466779.1"
                     /translation="MANTVDVAAALRELTSSKSLDRSGLIDLLKDGMHAALVKRYGPN
                     VRSEISIDEMKGTVRVVVLKTVVEAVEDPGSQVSLEEARFEDPDFAPGDVLEEEVPVE
                     QFGRTAVQAAKQRIIQRVREGERTRIREEFADKVGDLLSGEVQQIERGKLVVMLAKFR
                     EAEAIIPYREQNHREHFHQGDTIRAVLKRIEETPKGPRLILSRADPAFVKALFKLEVP
                     EIQQLIVEIRATARESGSRTKIAVASRDDAIDPVGACVGLKGSRVQAVVNELGGERID
                     IVPWSPDPERFAKLALAPARVARVFSDPDAKTIQAIVDEDQLSLAIGRNGQNVRLASE
                     LTGWKIDLYSSREWLERGGEGPLFAPLPPEEEQAVKVALREMKGLSAELVAVLEQNGK
                     KTLQDILDLERDDVTGMAGMTPVLADKLMGFLNELTEESGPEGGDDTAAPAPETPPA"
     gene            complement(12573..13040)
                     /gene="rimP"
                     /locus_tag="VFU75_07810"
     CDS             complement(12573..13040)
                     /gene="rimP"
                     /locus_tag="VFU75_07810"
                     /inference="COORDINATES: protein motif:HMM:NF014617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000028 - ribosomal small subunit assembly
                     [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEU5466780.1"
                     /translation="MLTDSLVEAFRVRLEALGYDLADLRIGGTAGRPLVQVRIDWPSP
                     KPNDPPQRVSVEDCGKASRVLEAWLDAEGPLGARYVLEVSSPGMDRPLRWPRHWVQFV
                     GRDVNAKIQGLGLVRARIVSVPDEKTVVLKFPGAEPRTVALAAIRDAHLAVDW"
     gene            complement(13874..15157)
                     /gene="murA"
                     /locus_tag="VFU75_07815"
     CDS             complement(13874..15157)
                     /gene="murA"
                     /locus_tag="VFU75_07815"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEU5466781.1"
                     /translation="MPSPAFVVSGGRPLTGTVRPAGNKNAVLPILAATILADGPCAID
                     NVPRIRDVETMLALLERLGADVQWTGINTVTVDPRKVEPKELDPALSARIRASILLAG
                     PMLARFGRVTLPPPGGDVIGRRRIDTHFLALERLGAEVTVGERYALEAKSLKGADIFL
                     DEPSVTGTENALMAAVGAKGTTIFRNAASEPHVQDLARFLVAMGAHIEGIGTNTITVR
                     GGIALRAAPHTVGPDHIEIGSFIGLAAVTNGAITIEGVRADDLRATLMAFERLGVRPR
                     ITGTALVVDADQERRVRSDLGGHIPELYDGPWPAFPADLMSIAIVTATQCAGMLLVFE
                     KMFESRLFFVDKLIGMGARIVLCDPHRAVIAGPSALRGQVVESPDIRAGMAILLAALA
                     AQGESVIHNIGQIERGYERIDERLRGLGAEIRRVE"
     gene            15220..16452
                     /locus_tag="VFU75_07820"
     CDS             15220..16452
                     /locus_tag="VFU75_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466782.1"
                     /translation="MNPVLALAVVLAAGLVVTRLPRIPHPTGLRLDLVASAGLPLVLL
                     GILLGPVLHLLDGDVLRALAPATALGIAWIGGVFGASLGWRYVRKVPPALWLLAGVEA
                     LAVLAVTALLGWELTRVIPALGPALSPTLPTVFALASIAVVTGPSAVAMTAHAVGIRR
                     SVRQAFEIAAWLDTAAGALAFILVLTIYHPRQPAGGVTLGWISWLGLGIASGLLFGFL
                     YLGLTHFLEDPEELHLGLLGVMLFGGGVGYAADLSPFVICAVAAVVIVNRSPHRHAAI
                     ALLRSWEHPVSAILLILAGALLTVPTLWILVMAVLLVWARAVARWLAVRVARGPLGLS
                     RVLPPDLGLAGVAQGAVAVALAMNFFILYGATVGGPVVTIALVGLGLNLLMAPAAMTR
                     ALKPAPLTAAPTAPEVSS"
     gene            16449..16868
                     /locus_tag="VFU75_07825"
     CDS             16449..16868
                     /locus_tag="VFU75_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466783.1"
                     /translation="MIPTWAAVVTALSLLVIALAAIVVAFAAVISALGVRATLGFFKQ
                     LAAPAVDDVRGLLGTIRTEADALVGTSKDIRMKIVQATDAAELRLSNLAALVDVLQEE
                     ADDVALDAAAAVRSVRRGVAAFSLGKRLFGKRKRKSR"
     gene            16880..17782
                     /locus_tag="VFU75_07830"
     CDS             16880..17782
                     /locus_tag="VFU75_07830"
                     /inference="COORDINATES: protein motif:HMM:NF013078.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc dependent phospholipase C family protein"
                     /protein_id="HEU5466784.1"
                     /translation="MVAVTVLAVLILFPAPLHAWTPGTHIYLGESVLANLHQLPTAVA
                     DLLRSFPFDFLYGNIAADTSMAKKYAPVGRHCHAWHVGQEIYDLAATDALRAFGFGYL
                     SHLAADTVAHNYFVPRQLVITSSTAALGHSYWESRFETHLGDAYARTAKEVIMRDHDA
                     SDAHLDGILSPTLFSIRTNRRLFRGMVHVTESQSWQWAFQMIAEASRWDLPTPDVDRH
                     MAVAFQYVMECLGDKDPATRRLDPSGEKALGMAKQMRREALAAGGREDPRQVAETAEE
                     HFGLPEVSLGYWRESKAQRPWTPA"
     gene            complement(17779..18102)
                     /locus_tag="VFU75_07835"
     CDS             complement(17779..18102)
                     /locus_tag="VFU75_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466785.1"
                     /translation="MKTRALTGLVLLAACGGSPKVFVANHAMSDRPTFEIEEGKATHI
                     DIVTCTDSTRTLWQADAPAGGYLPATVVDSTKALDEGCYMVRILPRHARHFEVMPDHV
                     LLQEL"
     gene            complement(18099..19535)
                     /gene="gatB"
                     /locus_tag="VFU75_07840"
     CDS             complement(18099..19535)
                     /gene="gatB"
                     /locus_tag="VFU75_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEU5466786.1"
                     /translation="MSASTAWETVIGLETHVQLGTVSKMFCGCSTTFGAPPNTNVCPV
                     CLGLPGALPVPNERAIQLAVRAAVALNCSVHPRSVFARKNYFYPDLPKGYQISQYDQP
                     LATAGHLSFLSPDRGVVRAGIIRVHVEEDAGKSLHDRFAKATAVDLNRAGVPLIEIVT
                     EPDFRSPQEARAYLLTLKQVLQYVGVSECDMEKGSLRVDANVSVRKAGSTALGVKCEV
                     KNINAFSYVEKALTVERDRQVAEIEKGGTITQSTLLYNSKTNTVRLQRSKEESKDYRY
                     FPEPDLPPLVLTPEWIAEQQGVLPELPATKRERFVSQYGLSQQDAGVLTAERSVADYY
                     ESVVHAGAEPKAAANWVMGEVLGDAKDHQEQLRVPASSLAGIIALVKGGTVSNQGAKK
                     VFAEVAAKGGDPRTVAEALGVIQVADTGVLAQWVEEVVAAHPAEAARFKGGETKLMAF
                     FVGQVMKTSKGKADPKAAPKLLEERLAK"
     gene            complement(19532..20917)
                     /gene="gatA"
                     /locus_tag="VFU75_07845"
     CDS             complement(19532..20917)
                     /gene="gatA"
                     /locus_tag="VFU75_07845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEU5466787.1"
                     /translation="MTEVEALASAKATATRLGKAKRLNALLTPTTVVRKSLLAQAKNA
                     HPSGVLYGMPVALKDNICTLEFPTTCGSKLLEGYQSPYEATVVTKLKDAGALIAGKAN
                     LDEFAMGSSTEHSAFGRVLNPVDRTRVPGGSSGGSAALVGAGAVPAALGTETGGSVRQ
                     PAAFCGVVGIKPTYGRVSRYGLVAFGSSLDQIGVLGRSVHDAARVLSVISGRDPRDNT
                     CENRDPLRLPTVPERLEGFVIGIPKEYFPVDLNPGVRRACDRAIRALKDLGAAVRDVS
                     LPHTAYAIPTYYVIAPAEASSNLARFDGVRYGNRAKDVTTLADLYRRTRGAGFGPEVR
                     RRILVGTYVLSSGYYDAYYKKAQQMRALLAQDFRNVFDRGVDLIFTPTTPTTAFKAGE
                     RTKDPVSMYLADCFTAPANLTGVPAMSLPIGRVKGLPVGGQFIGQPFLEDDMIEAAYA
                     LERAISGTEEV"
     gene            complement(20914..21228)
                     /locus_tag="VFU75_07850"
     CDS             complement(20914..21228)
                     /locus_tag="VFU75_07850"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF014716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEU5466788.1"
                     /translation="MSVTPEEVRQIARLAELSVDEEHLDDLAAQMSRIVDYIAQLSQV
                     SGGEHAKPFIPGPDLIRLRADVVAPQPLEGKLSDFAPAFREGFFLVPKLGVFEGAEEE
                     GE"
     gene            complement(21225..23492)
                     /locus_tag="VFU75_07855"
     CDS             complement(21225..23492)
                     /locus_tag="VFU75_07855"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEU5466789.1"
                     /translation="MTPPLNPTQRAAVEHGDGPLLVLAGAGSGKTRVLTARIAHLIKD
                     RGIRADRIFAVTFTNKAAGEMRRRVAELLGADPRGLWIGTFHSLSARLLRREAPLLGF
                     GPNFTIYDEEDSQALIKRMLEQRGHSPKAYPPRQLAGIISGAKNALLTPAEFSARAET
                     PLELVAADIYGGLGPALKQANAMDFDDLLLLPLELFRTHPDRLGYWQRRFVHVLVDEF
                     QDTNAAQYQLVRLLATLHGNLTVVGDDDQAIYGWRGADVRHMLAFQTDFPGTTMVKLE
                     QNYRSTQVILDAANGVIAENEGRLGKTLFTEKKGGSKVVLLTAADERDEAEWLAGEYA
                     KLASDDDQSYESMAVLYRTNAQSRPLEEAFRFRGIPYRLVGAVSFYERREVKDLLAYL
                     RLIANPADNEAFLRVINVPRRGIGDASVLALAQAGSKWGKPLLEVARVADRVEGLRPN
                     VRDGFARFAATLGNLRTAVGNEDPAFAFDRIIDAVHYDAYLADEGAEGVERMENVREL
                     IAGAAAWAEVSGIDGADEEEDGELALSGGTPIERYLTQSALMAQTDSVKGESGVTLLT
                     VHMAKGLEWPVVALAGLEDGLFPLARSAAQPGGVEEERRLCYVGLTRARERLYLSWAR
                     TRFRQGRMEMSEPSRFLETVPPAVIEERATTPLWDRGGYRQRFDRHSPPARRRFEERD
                     EAFFADEEDVSQDAPKYARGERVRHRKFGGGVVQAVSGGGRSLKITVLFDDPEIGAKQ
                     LLVAYAGLEREWETG"
     gene            complement(23499..24656)
                     /locus_tag="VFU75_07860"
     CDS             complement(23499..24656)
                     /locus_tag="VFU75_07860"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5466790.1"
                     /translation="MDRQDRWRRAGPAALIGAALLFGALSVADAWVVASHFQDDARNT
                     SRLFGRVFVGLSDPQPDAATGALLDLAGEVRAMHLPMAVTDATGHITQVANAPEGVEQ
                     DSVALAAWVRGLDEVNDPIVQPGIGTVHYGALPVARRLKVLALLQAAVLLCLIGLIWL
                     ANATKVGAARDRLWVAMARESAHQLGTPLMSLTGWIDYLRENPGTSGGELVEHLAADT
                     ERLQRVAKRFERIGRPARREPVGLGAAAERVVGYFRPRLPTLASPVQLVLHAIGPGPV
                     IDGDPVLVEWAIEALVKNAIDALSGRGGRIEVRVEAVGKTGRVVVEDDGPGVAAHIRS
                     HMFEPGRSTKPGGWGLGLALARRIVEQQHAGRVSYHPATPGSRFILDFPVP"
     gene            complement(24649..25935)
                     /gene="serS"
                     /locus_tag="VFU75_07865"
     CDS             complement(24649..25935)
                     /gene="serS"
                     /locus_tag="VFU75_07865"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003218092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEU5466791.1"
                     /translation="MIDLKRLRSSPDEVRAALARRGDASVMALLDELRGLDERRRTLS
                     GQLDALKSDRNKFALEDAAAVKQHGKLPKEIIDQRKEAGLKITALEHELRSIETVLSA
                     KALFVPNLPLGAVPDGGADANKVVRAWGTPRSESGLKPHWEIGEQLGLLDLPRGAKIS
                     GSGFPLLTGMGARLSRGLISFMLDLHTREHGYRELAPPFVVKRESMQGTGQLPKFEEE
                     AYKTAPDDLFLIPTAEVPVTNIHRDEILDGGKLPLAYTAYTPCFRREAGAAGKDTRGL
                     LRVHQFDKVELVRLVAPETSAAALEEITRHAATVLERLELPYRVVLLAAGDTGFGSAM
                     TYDLEVFAPGVGAWLEVSSASVFTDFQARRANIRFRPKPGEKPDFVHTLNASGVALPR
                     TIAALLEHGQEADGSVRLPKALVPYVGVDRLGPAGG"
     gene            complement(25939..26505)
                     /locus_tag="VFU75_07870"
     CDS             complement(25939..26505)
                     /locus_tag="VFU75_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466792.1"
                     /translation="MRPISSLCAGAALAAGVLSGACSSTLAPPAPSFVNVVDTATLYA
                     ATGTPVGTPSAYNIEDTVTGARVRTDTTPIFDFLFDIVGSQAKLYPPGALTGLARTSA
                     LQFASSFDGTTTAPGPGWNDSTALNVDSGTVVLVRSRQVSCAIGTVFQYAKLQILSID
                     TVARTTTFQILSDRNCGYRDLTPGYPKH"
     gene            26599..26952
                     /locus_tag="VFU75_07875"
     CDS             26599..26952
                     /locus_tag="VFU75_07875"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEU5466793.1"
                     /translation="MATIRDVVEALGKRPGVDAVLVVGRDGLAIDTHVVNGVDPESVS
                     ALLPGVIGAITQLGSAGGRGDFGTGVLEFGGGLAVVSVLTNDALLVVLVRPATNVGPL
                     LVDLRRHRSAIAGLF"
     gene            26960..27385
                     /locus_tag="VFU75_07880"
     CDS             26960..27385
                     /locus_tag="VFU75_07880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5466794.1"
                     /translation="MSRANSAPPGPLRPPRHLLVVDDEPHIGLLLRPEFESRGYRVSI
                     ARSLAEARTTVDSVDLMLLDVHLPDGSGIDFLRELRGRPRTQNLPILILTGESGDRVL
                     EEARLLGAGLLTKPFSPTKLGKSIAALIDGPAPVPGDAR"
     gene            27382..28398
                     /locus_tag="VFU75_07885"
     CDS             27382..28398
                     /locus_tag="VFU75_07885"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HEU5466795.1"
                     /translation="MSTWAAILAGGSGTRFWPLSTPDRPKQLLPLAGDRPLLAQAVRR
                     LTGLVPPERVLILTGPYLVEQVASAVPEVPRGQILAEPRTASTAPALVWAAQEIAKRD
                     PGAAMLSLHADWAVGDDAGFRASATKAIDVAQAHDVLVTVGVKPTRDETGYGYIVPGA
                     KLGKTPAKKVRRFVEKPSAARAKILRRGGALWNTGLFAWSTERFLTEARAHATELRAG
                     WHALDDGDVQGFFSRVTPVAVDVAVLERTKRLATLAGGFTWDDIGSWDALLRIRTPDA
                     HGNVVVGNVSLGDDVRRSVIWTDDEHLAVTGVTDMVVVRANGHTLVIPTGRAEKLKAL
                     VQKL"
     gene            28395..29570
                     /locus_tag="VFU75_07890"
     CDS             28395..29570
                     /locus_tag="VFU75_07890"
                     /inference="COORDINATES: protein motif:HMM:TIGR03991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar nucleotidyl transferase"
                     /protein_id="HEU5466796.1"
                     /translation="MSTAPALYLLDPEPAPAWAPFMGSRPIAELRAGAHLIRERWEAF
                     VGTETAAVFALPHLAGFTEPGSAPVRAREAVTGPAVIGSSTFAPRGLAPALPDEPSRL
                     VCGGVTVGWGVPAGARWDGPAREARAVEVPGVVLHGVHGLLPVLEALLREDLRGLLPG
                     TDGIPAGSTVIGDPGLVAVTEGLIEPGVVFDTRPGPVVLESGVEVRSGSRLEGPLWVG
                     ANARILGGSVRASAIGPRCTVRGEISTCVFLGYANKAHDGFLGHSVVGRWANLGAGTV
                     TSNLKNTYGPVRLEVAGHDIETGQMNVGSLIGDHAKTAIGTLLSTGTVIGTGASVFDV
                     VRPPKYIPAFAWGGTGDARTTREGFLKVAERVLPRRDIVMDEATRALLGRVYDWATR"
     gene            29567..31450
                     /locus_tag="VFU75_07895"
     CDS             29567..31450
                     /locus_tag="VFU75_07895"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU5466797.1"
                     /translation="MSDAETSPQPAPPKQGAAKPDAAFRRLHKFELHERIGEGAMGVV
                     WKAYDSILRRFVALKLLNPSFGRTQELRERFHREARAAAALQHPNIVTVYDLGESDGQ
                     LFIAMELVEGRDLSDLIAVRAPLPLEHKLDVVIELLQGLGYAHARGVIHRDVKPSNVR
                     IAADGRVKIMDFGIARLQNADRTGSGGVIVGTPSYMAPEQITNGEITPATDVFAVGCL
                     LYELLTYERPFEAETVHGVFYQVLSTTPKPLRTVAPSMPASLERVVLKAMAKTPGERY
                     GTAEEFRSSLLGMRHALSDPSSITTERIRFRNPFTRISLSLIAPASLKSRATVLLLLL
                     GAAVFIAYLALSTPAPAPASLPAASTVPILSPRPIPGLAPRIAILRDTAYAERARALE
                     AGGLINNVQPMLNADAILTAADQYARAKDLVRASTGYGSVGRLYRQAITETDSERLDA
                     KRRLDRAATLVRSMRGNPPTRISASLTRADSLFNARDYVMSAIASGATEQAALADGAR
                     MRTVTPQPRDPHKAILQLLDDFSRAIQSRKVSNMRVLYPSITKGEVKNWEDFFNGVTS
                     VDVTFTPDSITIKDPTARGVVHAHFLAIHGGSPTPQESQQTFETSFRHNPGGWRISGI
                     LSR"
     gene            complement(31452..31838)
                     /locus_tag="VFU75_07900"
     CDS             complement(31452..31838)
                     /locus_tag="VFU75_07900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466798.1"
                     /translation="MNRIVLGIVGALALPVIAGAQVAKRVHLEGQWEAKASDGLHYVM
                     VRADSSAQFGEQVARWRLVGDSIWFTLGDGAWQVYGLKVAGDKLTLSGGDLDKPVSLR
                     KVGPPTARPAGLVVPDAPPDTARAWD"
     gene            complement(31835..33049)
                     /locus_tag="VFU75_07905"
     CDS             complement(31835..33049)
                     /locus_tag="VFU75_07905"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEU5466799.1"
                     /translation="MSGDPIKFLGSLAQALAGMSLYAEGHPARDRGIDAAFEQLGGLQ
                     QDTATPLFTFLGDEVVYGKTPIRELKAWDWSMRLAQAGIQRLEFQPGVTREEFETFLD
                     EVLARLTLATIDTSEVRQQGKRSSIKFGSVGIRGEQRNPQAVLPVEATIRFSLGEEAE
                     AIRWMHEEVKAHGGLPLAEAEAVVRSLSVAMHGDQQIVLPLLHLKEFDQYTTTHSMNV
                     SVLAMALAEYMGMGAKDVRAFGVAGLLHDLGKTRIPLEILTKPGKLTPDEREVMNAHP
                     AQGARIILEAEEKLDLAAVVAYEHHIMLNGGGYPRPHYGRACHRASNLVHVCDVYDAL
                     RTKRPYRDAWEAEKILPYLEGRAGMEFDPEVVAPFIAMMRKWEPRVAVLQDEQAPVAP
                     TGVPTNGGQRPT"
     gene            complement(33046..>34854)
                     /locus_tag="VFU75_07910"
     CDS             complement(33046..>34854)
                     /locus_tag="VFU75_07910"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU5466800.1"
                     /translation="LAGRLHRHHLGAVSFARGIEGGELEEALRMLSSEPGRGVEAIGL
                     GPRDLIPHWDHVKLHPMTYDKLELLDDDGNPVGSKAERAKSAQLWIGLARAALAGGVE
                     DDDAASELKEAVVAEAINAHERGAAYDQVIVGYLLQIAEELKTAGGREAMELRKRTSR
                     MIAELRPETLQRLVDMGGNFAQRHKFVLDATHGMAVDAVIEIVRAAATSSHQTMSHSL
                     VRMLSKLAAHAEQGSALARPLADAALRDQVEQLMSGWQLDDPNPGAYGKALQEMSRAG
                     PIFATAPDAVFAPEEERLVEMALEIDATGPIVWGAVDRMVSGDQFSRLLELLEGAPAG
                     SATATTIRNRIFNVEAVARILAEPQPDFPLVDQLIQGVGVAAAAPLLDALATADVRAV
                     RRALLDRLGKMGEGFVPELLPRLKDQRWYVVRNLLALLDDLPTLPAGFSPAAFRVHPD
                     ARVRRQALKLELKHPTEREHALLAALADRDPQTLQMALMAIQTDCPPSTVPLLLQHAR
                     NDSLPSEDRVLAIKALGRSGAPEALLVLVDLTDGGRTLLGRAKLPAKSPELLAALQAL
                     AAGWGTDDRATAVLARAAQSADADVRAATDPGLGTA"
BASE COUNT         5368 a        12361 c        11318 g         5807 t
ORIGIN      
        1 ggctgaatga cctcgtcgat ctcctcccgc gtcgccgcgg gcaggcccgc accgatcacc
       61 cggttgaaga gcaggatatc aatcgtgggg acctgcagaa tccagcctcc cgcccgacgc
      121 tccgaccacg ccccccactc ccggggcgcg gccgccagca tgtccgcgaa ggcctgggcc
      181 tccgtgaact ccaggagatc ggaaatttcc gaagagatgg tcacccggtg gtcgcgacga
      241 cctcgatctc gacccgcgcg ttccggggca acccggccgc cgccaccgtc gaccgcgcgg
      301 gcttgtgatt cccgaaatgc ttcgcgtaca cctcattcat cgcctggaag tcggccatgt
      361 cggcgagaaa aaccgtcgtc ttcacgaccc ggtccagccc ggacccggcg gccttgagca
      421 ccgccgccag gttcttcatc acctgctccg tctgctcgcc caccgtcgtc cccacgagct
      481 ccatcttctt gggatcgaac cccacctgcc ccgccgtgta gacgaaaccg ttcgcgacga
      541 tcgcttgact gtaggggccg atagccccgg gcgcctggtc ggtcgccacc accttgagcg
      601 tcatgctaga agagtccctc gatggtcccg tccggatgca tccggatctt ctgggccgcc
      661 ggctccttgg acaggcccgg catcgtcatg atgtcaccgg cgagcgccac gacgaacccc
      721 gctcccgccg acggaagaat gtcccgcact tgcagcgtga acccgctcgg cgctcccagc
      781 ttggtcgcgt catccgagaa cgagtactgc gtcttcgcca tgcacaccgg cgtctccccc
      841 aaccccaact tcggcagcaa ctccatcgcc cgctcggccg ccggcgcgaa cgccaccgcc
      901 ccggccccgt agatctcctt cgcgatcgtt tcgatcttct ccctgatcgg cttcttctcg
      961 tcgtacagcg ggtggtagtt cgacttctcg gtatcgagca gcttgagcaa tgcacgggcg
     1021 acctcctcgc cccccgcccc acccttctcc cacacctcac accgcacgac ctccaccccc
     1081 tcggccgcgc acgccttttt caccgcggcg atctccgcgt cggtatccgt aatgaaccgg
     1141 ttcagggcca cgaccaccgg caacccgtac ttcttcacgt tctggacgtg ccgctgaagg
     1201 ttcacgacac ccttctccac cgccgccaca ttctcaggac ccaaggcatc cttcttcacg
     1261 ccgccatgca tcttgagcgc gcgcaccgtg gcgacgacca ccgccgcgtc cggcttgagc
     1321 cccgccatcc ggcacttgat gtccaggaac ttctcggcgc cgaggtcggc accgaatcct
     1381 gcttcggtca agacgaggtc ggcgagccgc aggccaagct gggtcgccat caccgagttg
     1441 cacccatgcg cgatgttgcc gaaaggcccg caatgcacga gcgcgggtcc cccctcgagc
     1501 gtctgtacga ggttgggctt gagggcgtcc ttgagcagga gcgtcatcgc gccctgcgcc
     1561 ttcagatctg aggcgcgcac cgcagtcttg ttcgccgtga gacccacgat gatccgggcg
     1621 agccgggctt cgagatcctc gatgctcgtc gccagggcca tgatcgccat cacttcggaa
     1681 ccggggatga tcacgaagcg ctcttcgcgc gccggtccgc cgttgatgcc gcccaacccc
     1741 acgatgatgg agcggagcgc gcggtcattc atgtcgatcg ttcgaggcca ggtgatgcgg
     1801 cgggtgtcga gcccggaagc gttgccatga tgcagatgcg agtcgagcat ggcggacagc
     1861 aggttgtgcg cgctcgagat cgcgtggaag tcgcccgtga aatggaggtt gatgtcctcc
     1921 atcggcacca cctgcgagta gccacccccc gccgcgcctc ccttcacccc gaacacggga
     1981 ccgagggacg gctcacgaat gcagaggatg gcgtcttttc cgatcttgcg gagcgcctgc
     2041 gtcagcccga ccgtgaccgt cgacttccct tctccggccg gtgtcgggtt gatccccgtc
     2101 acgaggatca gtttgccttt cggctttctt ttgctgatgg ctgacagctg tatcttcgcc
     2161 ttgtacttcc cgtaaagctc gacctcatcg tcggcgagcc ccagctcgcg ggcgatgtcg
     2221 acaacgggac gcatcttggc ggcttgcgcg atggcgatgt cggacggaac cgtggacaag
     2281 ggcgctcctc gaatcagggc gacggtgtga cgcgggcacc gaacacccgc gtcgcgttgg
     2341 cggtgagcat gggtacgaga gcctcgaggg tgaccccccg ggcccgggcc agcgccagcg
     2401 cggtttcccg cacgaacgcg ggctcgttgc ggctcccccg atgcggaacc ggcgccaggt
     2461 agggcgcgtc ggtctccacg agcaggcggt ccgcgggaca ccccgtcagg tactccgggt
     2521 gcccccagct cttgaacgtg atcatcccgc tgaaagaaaa gtacgccccg atctccatcc
     2581 ctgctgcaaa caggtccggt ccactgctga aggaatgcag gatgattgcc gggacccggg
     2641 caccccagga ccgcaggagc cgcgcgacgt cagcatcggc atcccgggca tgcacgacta
     2701 cgggcttccg gacgctttcg gccagcgcca gttgcccttc gaaactctcg atctgctggg
     2761 ggcgaggcga atgatcgtag tggtagtcga gcccggtctc cccgaccgcc accacctcgg
     2821 gctcggcgag aagaccgagc agctcggccc gggtggccgc ggaccactcc gtggcttggt
     2881 ggggatgaat ccctgcggtg gcggagagcc cggaagccga ccgggccagg gccagcgcat
     2941 cacggctggt cccgacccgg tctccgatca cgacgacatg gcccacaccg gccgcgcggg
     3001 cgcgggcccg cacgtcgtca cggtctccgt cgaacgcccc atccccgagg tggcaatggg
     3061 cgtcgaccag cacgtcaacg cgccgggctg ggtgtcggcg gagccgcctt ggcggccttc
     3121 ggttgcgtgg cgggagccgc ggctgccccg ggcgcgggca tggccatcgg cgacgtgagg
     3181 atccgggcac cccgcgcgtc ttcaccgggc cagcgcttct cgatgtacat caggatcggc
     3241 gccgcgatgt agatcgagga aaaagtgccg gtgaagatgg cgaacgccat caccagcgcg
     3301 aacgggcgga tcacctcgcc cccgagcagc gcgagcgccg tcgtcgtcgc gaacgtggtg
     3361 ccgtgcgtca gaatcgaccg cggcagcgtc tcgttgatcg aaagattgac gacgtcgtac
     3421 aggttctgac gccggaactt ccgcaagttt tcacggaccc ggtcgaagat gatgatggtg
     3481 tcgttcagcg agtacccgac catcgtcagc accgcgccga ccacgaccag gctgatttcc
     3541 aggttcatgt acttgatgaa cgcgatggtc gtgaggatgt cgtgtgccgt cgcgagcacc
     3601 gcggcggtgc cgaagcgcca ctcgaaccgg atcgcaaggt aggccagggt cacgaagaag
     3661 gaaaagaaga tcgccaggaa cgcctttccc tggagctcct tcccgacctt gccgtcgatg
     3721 ccctcgtatt tcatgacctt gtacttggtg ggaccgagtc ctctgtcgaa ggagctgcgc
     3781 accgcccgca tggtgctgtc cgaggcctgc tgggtgccgc tgttggtctc gccgagccgc
     3841 gcgcggatca tgaacgccgt gtcggcgccg taggaggaga tttcggcgcc atgcagcccg
     3901 ccggcgtcga gcgcggcgcg gatctgcgcc gccccgatcg cgtgatccag ggtcgcgatc
     3961 tggacctgcg tgcccccggt gaattcgatg ctgtagttcg gcggcgagat caggagccag
     4021 acgaaccccg gcacgatgag caacgcggac agcccgtacg cccacttgcg ccacttgatg
     4081 aagtcgtagc gcgcgttcgc gaagagtcgg aacatcagat gctcaccgta gtcatgtcgg
     4141 gccgccgctg cagccagatc atgtagaagg tccggacgac gaagatggcc gtgatcatcg
     4201 aggcggcgat gcccacgatc agcgtgatgg cgaagccctg taccgggccg gtgcccacga
     4261 ggtacagaat ggcggcggtg agcgcggtcg acacgttcga gtcgacgatg gcgctcatcg
     4321 cgtgcttgaa gccctcgtcg atggcgaggc gcaccgattt cccgtgctgg agctcctccc
     4381 ggatgcgttc gaagatcagc acgttcgcgt cgaccgcgat gcccaccgac agtgccaacc
     4441 ccgccagtcc cggcaaggtc agcgtgaacc cgaagatggc gagcccggcg agcgtgaaga
     4501 ggacgtagag cgccagcgcc gccacggcca ggaggcccga caggcggtag tagatgccca
     4561 tgatcaggat caccagggcg acgcccaccg ccccggccct gatgccgtca tgaatcgcgt
     4621 cggcgccgag cgtcggtccg atggactgct cgtcgacgat ggcgaggcgg acgggcagcg
     4681 cgccggcgcg gagcaccagc gccagatcct gcgcatccgc caggggtttg ctctgcagat
     4741 cgatctggcc atgcgccccg atctggtcgt tgatgatggg gggctgcccc tgcaccagcg
     4801 tgtcgaggat gatcgccatg tagtcgcgga cgtggcgccc ggtcgcatcc gcgaagaggc
     4861 gcccgccacg gcggctcaag tcgaactgca ccaccgagtg gttattgacg gggtcgcggc
     4921 gcgcggtcgc cttctcgagc tcgtcaccgg tgagcatcgc cttcgattcc acggcgtaga
     4981 ggtacttgta ttcgcgggca ccccggacca cggaatccgt gctccagaag aggtcgatcc
     5041 cccgcggcat cgcggtctgg aaggccgtca tctttcgcag ggagtccacg aggtgcgtct
     5101 gctccaccgg cacgaggtat tcgccgggca gctgcccctg gaacaggacg ctcgagaaga
     5161 cggccgagtt gttggtggtg tcgggagcgg ccttggcgcc tttcttcttt gaggaatcgg
     5221 cgccgaagag ctgcgaaacc gtgttcgacg gaccggcgtt ccccgtgccc ctcaccccgg
     5281 catcatggag ggctttgtcg atcccgggca gtgcgtcgcg gaacaggttc ttcatgtccg
     5341 tgatgcggaa ctcgaggtgg gcctgctgct gcacgatgcg tttggcgcgc tcggggtcgc
     5401 tttcaccggg aagctcgacg ttcaggcggc agttgccggt ggtctgcacc accggctcca
     5461 gggtcccgaa ctcgttgagc cgggtccgca ccactttctc cgaccgctcg atggcgtcct
     5521 tgcagtcggg caccgggccc tggctctggt ccacttcgag gtccaggtgc acgccgcctt
     5581 gcaggtcgag accgagggtg atcggcacca ggcgttcggt gaccaccacc atctgtccgc
     5641 tctgcggatc gagctgcttg acctgggtgg agcgtggcac gatggcgtac acggcgacca
     5701 ggaagagtgc gccgatcaag atcagccggt tccggatggt tttcatcgcg tccctgacag
     5761 gttgaggctt tcccgcgccg cggcgcggtc atgctcgagt tgtgcgatca atgcgtcgcg
     5821 ggacgggaaa gtacgcacgt cgcgcaggcg acgcacccat tccacccgca cccgctcgcc
     5881 gtacagatcc ccggcgaagt cgaacacatg ggtctcgagg ccgcgggccg tgacgccgaa
     5941 cgtcggccgc gccccctggt tcatcatgcc accgtaccgc cggccaccca cccaaacgtg
     6001 cacggcatat accccatcgg gcgggaggag cttcctggcc ggcggggtca ggttgatcgt
     6061 cggaatgccg atggtccggc cccggcccgc cccccgctcc acgactcccg agaaggcgtg
     6121 gggtctgccg agccagcgcg ccgcccggtc cagctcccca tgcgccaccg ccgtgcggat
     6181 caccgtcgac gatatcggct gcccgtcgtc ccgcaccgcg tccaccacgt ccacggtgaa
     6241 tccctgttcc agccccatcc ggcggaccgt ttcgacatct cccgagcgac cccgcccgaa
     6301 gccatgatca tggcccagaa ccaggtccac catcccgtat cgctcacgga gcacggtgcc
     6361 gacgaattcc gtcgccgaga gctgcgagag ctcccgggta aacgggagca cgacaacctc
     6421 gtccaccggt gatgccgcca gcacttcccg cttctcgtcc ggcgtcgtca acagcttcgg
     6481 cgccgcttcc ggattcacga cctcgagcgg gtgcgggtcg aacgtcacca gcaggctctt
     6541 ccgtcccgac ggcttcgccc gccgggcgat ctcggcgagt accgcctgat gcccgcgatg
     6601 gacgccgtcg aacgacccga ccgtgacgac ggtgccggct gtcccgctca tgcgtccgcc
     6661 aggacgaggc gcggtttgat gctcccgtcc accacttcgc cgaccgccag caggcagccg
     6721 tcagccgtca gcgttacggt tccgacgtca gccacccggg ccggaactgg gcgcccatgc
     6781 acgatggcct cccgttccgc cgcatcgaga gtccggctcg gcagatcacg gaccagcagg
     6841 accggatcct tgatgtcggc cggggtcagc gcctgcgccg ggaccgcatc ctcgagccgg
     6901 aagggcccga cgacggtgcg ccggagctcc gccaagtgcc cgccgcaccc caaccgctcg
     6961 cccaggtctc gcgcgaggga gcggagatac gtcccaccgc tcacggtcgc ccgaaacatc
     7021 acgtcgggcg ccttccagcc ggagagggtg aattcgaaca ccgtcacccg gcgcgcttcg
     7081 accaccaccg cttcaccacg ccgcgccttc ttgtaggcgc gcaccccgcc caccttcacc
     7141 gcggagaatg cgggtggctg ctgctcgatc tcacccgtca gtccggcgga cgcggcgcga
     7201 atctgctcga ggtcgagacc ccgccacgca tccgacgccg tcgtcacctc gccggtgcga
     7261 tcatcggtgg tcgtgaccga cccgagccta atcacgccct cgtaactctt cttccatccc
     7321 gccgcgtagg gcgcgagccg cgtcgcccgc cccaccacga tcaccagcag tccttcggcg
     7381 aagggatcga gggtgccgag gtgtccgacg cgcttctggt gcaaggcccg ccgggcgatg
     7441 tccacaatgt cgtgactggt cggccccgcg ggcttcgcca ccagacacac gccgtcggtc
     7501 aggactcgcc ctcctcgctc cccgaccgca ggtccttcaa gacctgattg atccgctggg
     7561 catgctccag gccccggtcc agccggaagt ggagctccgg gctggtgcgc agcgtcagct
     7621 ccttcgagag ctgggcccgg aggaaccgcg ccgcgctggc cagtccctcg agggagcgcg
     7681 ttttctcctc ttccgtgccc atgacgctca cttgcacgtt ggcgtgcgag agatcggccg
     7741 taacctccac tccggtcacg gtgacaaatc cgatccgtgg atcgcgaacg tcgccggtca
     7801 gaaacgcccc cacggtctgc cgcaccagct cggcgacccg ctcgggacga tggcttctgc
     7861 gtttcatcat cagagataac tcgtgctcgt gtccacgatc cgcgcgccgg cggtttcgtc
     7921 caccagccgg tccgcttcac ggagcacctt ctccgcgtgc acccggtcgg tgctcatcac
     7981 gcaagcggac agctccgcgc gctgccagag gtcctgatgg gccgtctccg ccaccgagat
     8041 gttgtacttc cggtgcagcc ggtccttcag gctttcgagc acctgccgct tgtccttcag
     8101 ggagtggcag gcatccaggt gcagctccca cgtcatgata ccgacgatca atcaggtggc
     8161 cttggcggcc gtgcccccgg agagcgtccg ggcgacctcg tcgatccggt acgcctcgat
     8221 caagtccccg accttcacgt cgttgaaatt ctcgacgccg atgccgcatt cgaagccttc
     8281 acgcacttcg cgtgcgtcat ccttgaagcg cttcagcgac gcgagcgtgc cgtcgtacac
     8341 ttcgacgccg ttgcggatca cccgcacctt cgcggtccgc gggatgacgc ccgagcgcac
     8401 gaacgaaccc gcgatggtgc cgaccttcgc gatcttgaag acctgccgca cttccgcctc
     8461 gcccagcacc acttccttct tctccggcgc caggaggcct tccatggcgg acttcacttc
     8521 gtccaccgcc tcatagatga tgcggtaggt ccggatttcc acccgctcac gctccgccga
     8581 cgcgcgcgcg ttggagtcgg gacggacgtg gaagccgatg atgatggcgt tcgaggcctt
     8641 cgcgaggagc acgtccgagt cggtgatcgc gcccacgccc cggtgcacca cttcgacctt
     8701 gacctcggac gtcgacagct gggccagcgc gtcggcgagc gcttccgccg ggccgccctg
     8761 gtccgccttg atgatgatct tgagcgacgc cgcgccgccg tccacccgct cggccatgaa
     8821 gtcttcgagc gtcttgaccc ggccggtgcg gcgatgctga gcttcgcgct cgagccgctg
     8881 gcgcttctgc gcgatttcgc gcgccgcgcc cgcatcctcc atcacggtga acgtgtcccc
     8941 cgcctccggc acaccctcga acccgaggat ctgcaccggg atggacggac ccgcgtcctt
     9001 caccgtctgc ccgcgctcgt cggtgaccgc gcggacccgg ccggagaact ggccgcagat
     9061 gaaatcgtcg ccgacgcgga gggtgccgtt ctgcacgagg atcgtcgcga cgggtccctt
     9121 ccccggatcg agcgtcgctt ccagcacggc gcccgcggcg cgacggtccg ggttcgcctt
     9181 caggtcgagg agttcggcct ggagaaggat ctgatcgagc agtttgtcga cgcccgtgcc
     9241 cttcttggcg gagatggggg tcatgagcgt cgtgccgccg aactcctcga gcaccacgcc
     9301 gtgcgccagc agatcctgtt tcactttctg cacattcgcc gacgggagat cgatcttgtt
     9361 gatggcgacc accagcggca cacccgcgtt cttcgcgtgc gaaatcgcct cgatggtctg
     9421 cggcatgatg gcgtcatcgg ccgcgacgac gagcacgacg atgtccgtca cctgggcgcc
     9481 gcgggcacgc atggcggtga acgcttcgtg acccggcgtg tcgaggaacg tgatcgtgcg
     9541 cccgtctttg agcgtgacgt gataggcgcc gatgtgctgg gtgatgccgc ccgcctcacc
     9601 ggcgacgacg ttggcctgcc ggatgtaatc gagcagtgac gtcttgccgt ggtcgacgtg
     9661 acccatgacg gtcacgatcg gcggccgcgg cttcagggtc tcggcggcat cggcggcttc
     9721 ttccggcgct ccgctcgaaa cgtactcctc ttcctgcacc gcctggaatc cgaacgccga
     9781 cgcgatcagc tcgatcatgt cgaagtcgag ccgctgattg atcgtcacca tctgcccgag
     9841 ctctttgaag gcgaacgtga cgatctgcgt cgggggaacc ttcaaggtgc tcgccagctc
     9901 ggagacggtg atgaactccg tcacccgcac cagcgtcttc tcgcgttcct tttgctcctg
     9961 ccgctgctgt tcctggatgt cgcggtagga aggtccctct tcgcggcgat gcggaccctt
    10021 ccgccccacc ggtcccttga gggccgagag ggtgcgggag atgttctgcg ccaccgcttc
    10081 ctgatcgacg gatttcttcc ccttcttccg cttcttgccg cccttgtcgc gccgacggtc
    10141 gtcgagaccg ggccccccgc cgccaccggg accaccggct cccggacctc cgaaattcgg
    10201 catgccgctc gccaccggtc ggggcggcat cggcgttccg ggcgtgtggc tggccacggg
    10261 cttgggacgg acgggcgtcg ggatgaacgg acgcggcgga gggggcgcgc tcggagccga
    10321 gggcgcgggc ggagccaggg gaggctcgac caccgccgag gcggctgccg gctgtagcgg
    10381 atcggccgcg cggggcagat ccttgaagag ctgcgcagcc cgctcctcca gcgaaaccac
    10441 cggcttctct tcgatctgcg ccttggcggc cgcctcgagc gcgtcccgcg cccgctgctc
    10501 ggccagggct tcgacctcgg cggcggcgga ggcttccgcc tcggcggccg cgacttccgc
    10561 cgcggtccgg cggcggcgct tgggacgcgc ctcgacgggc tcgggtacgg gttccggagc
    10621 gaccttcacg gcgcgccgtc tcttggtcgg ggcggcgggc tccttggact tccgcttgtc
    10681 ccgctcccag cgtgcccgca ccagggccac ctgctcgtcc ttgagcggcg agaggtgact
    10741 ccgtacgaac acgtccatct ccttgagcat gcccatcaac tgctcggcgg agatgccgaa
    10801 ttcggctgcc agatcatgaa tccgcgtcgt tcccactcta ctcctccgaa agccctaaca
    10861 cgcctcgcgc caacgaggga tcgttgacgc cgattgcctg cacgggcgga cgtcccaacc
    10921 ccgcccccaa cgccttcgca tccggaccga cgatcacggg cacattggtc gcccgcgcca
    10981 gccgttccac cttgtcgcgg gtccgttcgc cggcatccga cgccagcacc acgcaccgca
    11041 cgccgccgcg atgcagctcg gcccgggttc ccgacacacc gaccactacc gtacgcgccc
    11101 gcacgcccag tcccagaagc cggaccagcc gggccgggtc gctcacgccg gcggcgtttc
    11161 cggcgccgga gccgcggtgt catccccgcc ttcgggaccg ctctcctccg tcagctcgtt
    11221 caggaacccc atcagcttgt ccgccaggac cggtgtcatg cccgccatgc cggtcacgtc
    11281 atcgcgctcg agatcgagaa tgtcctgcaa cgtcttcttg ccgttctgct ccagcaccgc
    11341 gaccagctcg gccgagaggc ccttcatctc gcggagcgcg accttcacgg cctgctcttc
    11401 ttcaggaggc agcggcgcga agagcgggcc ttcgccgcca cgctcgagcc actcacggct
    11461 cgagtagagg tcgatcttcc acccggtgag ctccgacgcg agccggacgt tctgtccgtt
    11521 gcggccgatg gcgagtgaca gctggtcctc gtccacgatg gcctggatgg tcttggcgtc
    11581 cggatccgag aacacgcggg cgacgcgcgc gggcgcgagg gccagcttgg cgaagcgctc
    11641 cggatcgggg gaccagggca cgatgtcgat gcgctcgccg ccgagctcgt tcacgaccgc
    11701 ctggacgcgg gagcccttga ggcccacgca cgcgcccacc ggatcgatgg cgtcgtcccg
    11761 gctcgcgacg gcgatcttgg tccgtgatcc ggactcgcgg gccgtggcgc ggatctcgac
    11821 gatcaactgc tggatctccg gcacctcgag cttgaagagc gccttcacga acgccggatc
    11881 ggcgcgcgac agaatcagcc gcgggccctt gggcgtctct tcgatccgct tgagcaccgc
    11941 gcggatggtg tcgccctgat gaaagtgctc gcgatggttc tgctcgcggt acgggatgat
    12001 cgcttcggct tcgcggaact tggcgagcat caccacgagc ttgccgcgct cgatctgctg
    12061 gacttccccg gagagcagat caccgacctt gtcggcgaat tcttcacgga tacgggtccg
    12121 ctcaccctcg cgcacccgct gaatgatccg ctgcttcgcg gcctggacgg ccgtccgtcc
    12181 gaactgctcg accggcactt cctcttccag gacgtccccg ggcgcgaaat ccggatcttc
    12241 gaagcgcgcc tcttccaggg agacctgcga gcccggatcc tcgaccgctt cgaccacggt
    12301 cttgaggacc actacccgca cggtgccctt catttcgtcg atcgaaatct ccgaccggac
    12361 gttcgggccg tagcgcttca ccagggccgc gtgcatcccg tccttgagaa gatcgatcag
    12421 gccgctgcgg tcgagcgatt tcgagctggt gagctcgcgg agcgccgccg cgacgtcgac
    12481 tgtgtttgcc atgttgtgaa gttcctgtgt tagccgtcag ccgtcagcca tcagcaggcc
    12541 aatgccgtct gctgacggct gatggctgat ggctaccaat ccactgccag atgcgcatcc
    12601 cggatcgcgg ccagcgcgac ggtgcgcggc tctgcccccg ggaacttcaa caccaccgtc
    12661 ttctcgtccg gcaccgacac gatccgggcc cgcaccagcc ccagtccctg gatcttggcg
    12721 ttcacatccc gccccacgaa ctggacccag tgccggggcc accggagcgg ccgatccatt
    12781 ccggggctcg agacctccag gacgtaccgc gcccccagag ggccctcggc gtcgagccag
    12841 gcttccagga cgcggctcgc cttcccgcaa tcctccactg acaccctctg aggggggtca
    12901 tttggcttgg gggacggcca gtcgatgcgg acctggacga ggggccggcc tgcggtgccg
    12961 ccgattcgga ggtctgccag atcgtagccc aaggcttcga gccggacccg gaaggcctct
    13021 acgagactgt cggtgagcat aaaaaaaagg gcgggcctga cttcgcccgc tgcaggccgg
    13081 aatgtaccct gggtccaacc caagtcaagg cgtcaagtct ttgggcgacc taggtatgcg
    13141 accatccgcc ccggtttgcc gcccgagccc cggtgggaat gaaggctggc gtcatgagcg
    13201 gtgcaaagag gagacatcga gatctcccgg attcccagtc cccaggcctg ttcagccaga
    13261 tgcgaccgga ggtcaacgcg agtcttggtg ccctccggga tcgactcgcc aagcgcattt
    13321 gccacctcag aacctacttc ataacaagca ccgcaaatac ctatgccaca atgtattaca
    13381 agacttgata tcgacgccat cgcgacatct gccaggacct ttgctcctac cgcgagtatg
    13441 ccgccagcgg tgccccgcca ccccgcatgg acgagagcga ctcctccgga ctgggggtcg
    13501 gccatataca cggggatgca gtccgccacg ctgatggtca ggaggatgcc tgtcgaggcc
    13561 gtggcgtgcc cgtcccagcc ctccccaatg agccaaccat ccggaactgc ctcgtaccgg
    13621 cggaccaggg tcccatggac ctggcgtgaa aagaccaccg cgggcagccg ggggcggaaa
    13681 tgggcctgaa aagctcgcca ccggtccacg gaaaacccta ccggagcgtt gccggagagc
    13741 tcgagcgaga agccatcgtg cgcggtcgtg atccccgcga cgaggccgaa gcgagtggcc
    13801 cactccgcga gctcgagtct gggaatccca tccgaggggc cggcaggagg ggcctcccgg
    13861 agcaccgacg tgatcactcg acccgccgga tctccgcgcc gagtccccgg aggcgctcgt
    13921 cgatgcgctc gtagccgcgc tcgatctggc cgatgttgtg aatcaccgac tccccttgag
    13981 cggcgagcgc cgccaggagg atcgccatgc cggcccgaat gtccgggctc tcgaccacct
    14041 ggccacggag cgccgagggc ccggcgatga cggcgcgatg cgggtcgcag agcacgatgc
    14101 gggcgcccat cccgatcagt ttgtcgacga agaagagccg cgattcgaac atcttctcga
    14161 acaccagcag catgcccgcg cactgggtcg cggtcacgat ggcgatcgac atgagatcgg
    14221 ccgggaaggc cggccagggg ccgtcataga gttccggaat gtggccaccc aagtcgctcc
    14281 gcacgcgccg ctcctgatcg gcgtcgacca cgagcgcggt tccggtgatg cgcggccgta
    14341 cgcccagccg ctcgaaagcc atcagggtgg cgcggaggtc gtcggcccgc acgccttcga
    14401 tcgtgatggc gccattcgtc accgccgcaa gcccgataaa cgagccgatc tcgatgtgat
    14461 cgggccccac ggtgtgcggt gccgcccgca acgcgatgcc cccccgcacg gtgatggtgt
    14521 tcgtcccgat cccctcgatg tgggccccca tcgctaccag aaagcgcgcg aggtcctgga
    14581 cgtgcggttc cgacgcggca ttccggaaga tggtcgtgcc cttggctccc acggccgcca
    14641 tcagcgcatt ctcggtcccc gtgacgctgg gttcgtcgag gaagatgtcg gcgcccttga
    14701 ggctcttcgc ctcgagggcg tagcgctcgc ccacggtcac ctcagcgccc agccgctcga
    14761 gggccaggaa atgggtgtcg atccttcgcc gcccgatcac gtcaccgccc ggaggcggca
    14821 acgtcacgcg tccgaaccgc gcgagcatcg gtcccgcgag caggatcgac gcccgtatcc
    14881 gggccgagag ggctggatcg agctccttcg gctcgacctt ccggggatcg acggtaacgg
    14941 tgttgatgcc ggtccattgg acgtcagcgc cgagccgctc gaggagcgcg agcatggtct
    15001 cgacgtcgcg gatgcgcggc acgttgtcga tcgcgcaagg accgtcggcg agaatggtgg
    15061 cggcgagaat gggcaggacg gcgttcttgt tccctgccgg acgcaccgtt ccggtgaggg
    15121 gtcggccgcc ggaaacgacg aacgcaggtg agggcattgg tgccggaacg atatggcgcg
    15181 gcttgcgcgc cagtcaagcg ttcgcgaacc ttcgccgacg tgaatcccgt cctcgcgttg
    15241 gccgtggtgc tggccgcagg gttggtcgtc acgcggctcc cacgcatccc ccaccccact
    15301 gggctccggc tcgacctcgt cgcctccgcc ggcctgccgc tcgtgttgct cggcatcctg
    15361 ctcggacccg ttctgcacct cctggacggg gacgtcctcc gggctctggc gccggcgacg
    15421 gcgctcggca tcgcctggat cggcggggtg ttcggcgcgt ccctgggatg gcggtacgtc
    15481 cggaaagtgc cgccggccct ctggctcctc gccggagtgg aagccctcgc ggtcctcgcg
    15541 gtgacggcac tgctcgggtg ggagttgacc cgggtgattc ccgcgctcgg acccgcgctg
    15601 agccccactc ttcccaccgt gttcgcgctc gcgtcgatcg cggtggtcac cggcccatcc
    15661 gccgtggcga tgaccgccca cgccgtgggc atcaggcgtt cggtccgcca ggccttcgag
    15721 atcgccgcct ggctggacac cgcggcgggc gcgctggcct tcattctcgt gctgaccatt
    15781 taccacccgc ggcaacccgc cggtggcgtc accctcggct ggatttcctg gctcgggttg
    15841 ggcatcgcga gtggtctcct gttcggattc ctgtatctgg ggctcacgca tttcctcgag
    15901 gaccccgaag agctgcatct gggcctcctc ggcgtcatgc tcttcggcgg cggcgtcggg
    15961 tatgcggcag atctctcgcc cttcgtgatc tgtgccgtgg cggcggtcgt gatcgtgaac
    16021 cgttcgcccc atcgtcacgc cgccatcgcg ctcctccggt cgtgggagca tccggtgtcc
    16081 gccatcctgc tgatcctcgc gggcgcgctg ctcaccgtcc cgacactctg gatcctggtg
    16141 atggcggtct tgctggtctg ggcgcgcgcg gttgcgcgct ggcttgccgt gcgggtggct
    16201 cgcgggcccc tgggactgag ccgagtgctc ccgcctgacc tggggttggc gggagtcgct
    16261 cagggggcgg tcgcggtcgc cctggccatg aacttcttca ttctgtacgg cgccacggtc
    16321 ggcggcccgg tcgtcaccat agcgctcgtg ggcctcggat tgaaccttct catggccccg
    16381 gccgcgatga ctcgcgccct caaaccggcg ccgttgacag ccgcacccac cgcgcccgag
    16441 gtttcctcat gattccgacc tgggccgccg tcgtcacggc gctatcgctg ctcgtgatcg
    16501 ctctcgccgc gatcgtcgtc gccttcgccg ccgtcatctc cgcgctcggc gtccgtgcca
    16561 ccctcgggtt cttcaagcag cttgccgctc ccgcggtgga tgatgtgcgc gggctcctcg
    16621 gcaccatccg caccgaagcc gatgccctcg tgggcacgtc gaaggacatc cggatgaaga
    16681 tcgtgcaggc caccgatgcg gcggagctgc ggctgtccaa cctcgcggcg ctggtggacg
    16741 tgctccagga agaggcggat gacgtggcgc tcgatgcggc agccgcggtt cggtcggtcc
    16801 gccgcggagt cgccgcgttc tccctcggca agcggctctt cggcaagcgc aagaggaaat
    16861 cacgctgaag acgtggctcg tggtggccgt gacggtgctc gcggttctga tcctgttccc
    16921 cgcgccgctc cacgcctgga cgcccggcac acacatctat ctcggcgaat cggtcctcgc
    16981 gaacctccac cagctcccca ccgccgtcgc cgacctgctc cggtcttttc cgttcgattt
    17041 cctctacggc aacatcgccg ccgacacgtc gatggccaag aagtacgcgc cggtgggccg
    17101 gcactgtcac gcgtggcacg tgggacagga gatctacgac ctggccgcga ccgatgcgtt
    17161 gcgggcgttc ggcttcggct atctgtcgca tctcgccgcc gataccgtcg cgcacaatta
    17221 cttcgtgccg cgccagctcg tgatcacgtc gagcacggcg gccctcgggc acagttactg
    17281 ggagagccgc ttcgaaaccc atctgggcga cgcgtacgcc cggaccgcca aggaagtgat
    17341 catgcgcgac catgacgcga gtgacgccca cctcgacggc atcctctcgc cgacgctctt
    17401 ctcgatacgc accaaccgcc gcctgtttcg cgggatggtg cacgtcacgg agagccagag
    17461 ctggcagtgg gcattccaga tgatcgccga agcgagccgc tgggatctgc cgaccccgga
    17521 cgtcgaccgg catatggcgg tcgcctttca gtacgtgatg gagtgtctgg gcgacaagga
    17581 tcccgcgacg cggcggctcg atccgtccgg ggagaaggcg ctcgggatgg cgaagcagat
    17641 gcgccgggaa gcactggcgg cgggtggccg ggaggatccg cgccaggtgg ccgagacggc
    17701 ggaagaacat ttcggactgc ccgaggtttc cctcggctat tggcgggaat ccaaggctca
    17761 gcgcccctgg acgcccgctt agagctcctg cagcaagacg tggtcgggca tgacttcgaa
    17821 atgccgtgcg tgacggggca ggatccgcac catgtaacac ccctcgtcga gcgccttggt
    17881 ggaatccacc accgtcgccg gcaggtaccc acccgctggg gcatccgcct gccacagcgt
    17941 cctggtcgaa tccgtgcagg tgacgatgtc gatgtgcgtg gccttgcctt cctcgatttc
    18001 gaaggtcggg cggtcggaca tcgcgtggtt cgcgacgaag accttcggcg atcccccgca
    18061 agcggccagc agcacgagtc cggtgagtgc gcgcgttttc atttcgcgag cctttcctcg
    18121 aggagtttcg gggcggcctt cggatcggcc ttgcccttgg acgtcttcat cacctgcccg
    18181 acgaagaagg ccatgagctt ggtctcgcca cccttgaagc gggcggcctc cgccgggtgc
    18241 gcggcgacca cctcttccac ccactgcgcg aggaccccgg tgtccgccac ctgaatcacc
    18301 cccagcgcct cggcgacggt gcgggggtct ccgcccttcg ccgcgacttc ggcgaagacc
    18361 ttcttggcgc cctgattcga gacggtcccg cccttcacca aggcgatgat ccccgcgagt
    18421 gacgacgccg gcacgcgcag ctgttcctga tgatccttgg cgtctcccag gacctccccc
    18481 atcacccaat tcgccgcggc cttcggctcc gcccccgcat ggacgaccga ttcgtagtag
    18541 tcggccacgg agcgctcggc ggtgagcacg ccggcatcct gctggctcaa cccgtactgc
    18601 gacacgaacc gctcccgctt cgtcgcgggg agctccggca acacgccctg ctgctcggcg
    18661 atccactcgg gggtgagcac gaggggcggg agatcaggct ccgggaaata ccggtagtcc
    18721 ttgctctctt ccttggaacg ctgcagccgg accgtattgg tcttggagtt gtacagcagg
    18781 gtcgactgcg tgatggtgcc gcccttttcg atctccgcga cctgccggtc gcgctcgacc
    18841 gtcagggcct tttcgacgta cgagaacgca ttgatgttct tcacctcgca cttcacgccg
    18901 agcgccgtgc tgcccgcctt gcggaccgag acgttcgcgt cgacgcgcag gctgcccttc
    18961 tccatgtcac actcggacac cccgacgtac tgcagcacct gcttcagcgt gagcaggtac
    19021 gcgcgcgctt cctgcgggga ccggaaatcg ggttcggtga cgatttcgat gagcggcacg
    19081 ccggcgcgat tcagatccac cgcggtcgcc tttgcgaagc ggtcgtgcag ggacttgccc
    19141 gcatcctctt ccacgtgcac ccggatgatc ccggcgcgaa cgaccccgcg gtcgggagac
    19201 aggaacgaca ggtggcccgc ggtcgcgagc ggctgatcgt actgcgagat ctgatagccc
    19261 ttcgggaggt ccggatagaa gtagttcttc cgggcgaaca cgctgcgggg atgcacggag
    19321 cagttgaggg cgacggccgc gcgcaccgcg agctggatcg cccgctcgtt cggtaccggc
    19381 agcgcgccgg gcaggccgag gcagacggga cacacgttgg tgttcggcgg cgcgccgaac
    19441 gtcgtcgagc acccgcaaaa cattttcgag acggtgccga gctgcacgtg ggtctcgagc
    19501 ccgatgaccg tttcccaggc ggtggaggcg ctcacacctc ctccgttccg gaaatcgccc
    19561 gctccagcgc gtaggccgct tcgatcatgt cgtcctcgag aaacggctgg ccaatgaact
    19621 ggccgccgac cggcagtccc ttcacccgcc cgatcgggag cgacatcgcc ggaacgcccg
    19681 tgagattcgc gggcgccgtg aagcagtcgg cgaggtacat cgataccggg tccttggtcc
    19741 gctcccccgc cttgaacgcc gtggtcggcg tcgtcggcgt gaagatcagg tcgaccccgc
    19801 gatcgaagac gttgcggaag tcctgcgcca ggagcgcgcg catctgctgc gctttcttgt
    19861 agtaggcgtc gtagtagccc gacgacagca cataggtacc gaccaggatc cgccgccgca
    19921 cctccggacc gaacccggcc ccgcgcgtcc gccgatacag gtcggccaac gtcgtcacgt
    19981 ccttcgcgcg gttgccgtac cggacgccgt cgaaccgcgc gaggttggag gacgcctcgg
    20041 cgggtgcgat gacgtagtag gtcggaatgg cgtaggcggt gtgcggcagg gatacatccc
    20101 gcaccgccgc gccgaggtcc ttcaaggcac ggatggctcg gtcgcaggcg cgacgcaccc
    20161 ccgggttcag atcgaccggg aagtactcct tcggaatccc gatgacgaac ccctccaggc
    20221 gttcgggcac cgtggggagt cgaagggggt cgcggttctc gcaggtgttg tcccggggat
    20281 cccgccccga aatcacggag agcacgcggg ccgcgtcgtg cacgcttctc cccagcacgc
    20341 cgatttgatc gagagatgat ccgaacgcca cgagcccgta gcggctcacg cgtccgtacg
    20401 tcggcttgat gccgacgaca ccgcagaacg cggcgggttg gcggaccgaa ccgccggttt
    20461 cggtacccaa ggccgcgggg accgcaccgg caccgacgag ggccgccgag ccccccgacg
    20521 agccgcccgg aacccgggtg cggtcgacgg gattgagcac gcgcccgaac gccgagtgct
    20581 cggtcgacga tcccatcgcg aattcgtcga ggttggcttt ccccgcgatg agcgcgccgg
    20641 catccttcag cttggtgacg acggtggctt cgtagggtga ctgatagcct tcgagcaact
    20701 tcgacccgca cgtcgtcgga aattcgagcg tgcagatatt gtccttcaac gcgaccggca
    20761 tcccgtagag cactcccgac gggtgcgcgt tcttggcctg ggcaagcagc gacttccgca
    20821 ccaccgtcgt gggcgtcagg agggcattga gtcgcttggc ttttccgagg cgggtggccg
    20881 tggccttggc gctcgcgagc gcttcgactt cggtcactct ccttcctcct cggccccttc
    20941 gaacacgccg agcttgggca ccagaaagaa gccttcacgg aaggcgggcg cgaagtcgga
    21001 cagctttccc tcgagcggct gcggcgcgac gacgtccgcg cgcagccgga tcaggtcggg
    21061 accggggatg aacggcttcg cgtgctcgcc cccggacacc tgcgaaagct gcgcgatgta
    21121 atccacgatg cgcgacatct gcgcggcgag atcgtccagg tgctcttcgt ccaccgagag
    21181 ctccgccagc cgcgcgatct gccgcacttc ttcgggggtt acgctcatcc ggtctcccac
    21241 tcgcgctcga ggcccgcata ggccacgagc aattgtttgg ctccgatctc cggatcgtcg
    21301 aacagcaccg tgatcttcaa gctccggccg ccgccggaga ccgcctgcac gaccccgcca
    21361 ccgaatttgc ggtgacggac ccgctcgccc cgcgcgtact tcggcgcgtc ctgggacacg
    21421 tcctcttcat ccgcgaagaa cgcctcgtcg cgctcctcga atcgcctgcg cgccggcggc
    21481 gaatggcgat cgaaccgctg ccggtacccg ccgcgatccc acagcggcgt ggtggcacgc
    21541 tcctcgatca ccgccggcgg cacggtctcg aggaaccgcg agggctcgga catctccatc
    21601 cgcccctggc ggaaacgcgt gcgcgcccaa gagagataca atcgttcgcg ggcgcgcgtc
    21661 agtcccacgt aacagagtct ccgctcctcc tccaccccgc ccggctgcgc cgccgagcgc
    21721 gcgagcggga agaggccgtc ttcgagcccc gcgagcgcca ccaccggcca ctccagcccc
    21781 ttcgccatgt ggaccgtcaa caacgtgacc cccgactccc ccttcacgga atcggtctgg
    21841 gccatcaggg ccgactgggt caggtaacgc tcgatcggcg tccctcccga cagggccagc
    21901 tccccatcct cctcctcgtc cgccccgtcg atcccggaga cttccgccca cgcggccgcc
    21961 ccggcgatca gctcccgcac gttttccatg cgctccaccc cctcagcccc ctcatccgcc
    22021 aggtacgcgt catagtgaac cgcgtcgatg atccggtcga aggcaaaggc ggggtcctcg
    22081 ttccccacgg cggtgcggag gttccccagt gtggccgcga acctcgcgaa gccgtcacgg
    22141 acgttggggc gcagaccctc cacccggtcg gccacccggg cgacttcgag gaggggtttc
    22201 ccccacttcg agcccgcctg cgcgagggcg agcaccgagg cgtccccgat accccggcgg
    22261 ggcacgttga tcacccgcaa gaaggcctcg ttgtcggccg gattggcgat cagccggagg
    22321 tatgcgagga ggtccttcac ctcccggcgc tcgtagaagc tgacggctcc caccaggcgg
    22381 tacgggatgc cgcggaagcg gaacgcctct tccaggggcc gggattgggc gttggtgcgg
    22441 tagaggacgg ccatcgattc gtacgactgg tcgtcgtcgc tcgcgagctt ggcgtactca
    22501 cccgccagcc actcggcctc gtcccgctcg tcggcggccg tgagcagaac gaccttggaa
    22561 ccgcccttct tctcggtgaa cagggtcttc ccgagacggc cctcgttctc ggcgatcacc
    22621 ccgttcgcgg cgtcgagaat cacctgggtc gagcggtaat tctgctcgag cttcaccatc
    22681 gtggttccgg ggaaatccgt ctggaacgcc agcatgtgcc tgacatcggc gccccgccac
    22741 ccgtagatcg cctgatcgtc gtcccccacc acggtcagat tgccgtggag ggtcgccagc
    22801 agccgcacca gctggtactg cgcggcgttg gtatcctgga actcatccac cagtacgtgc
    22861 acgaaacgcc gctgccagta gcccaaccgg tcgggatgcg tgcggaacag ctcgagggga
    22921 agcaggagca gatcgtcgaa atccatcgcg ttcgcctgct tgagcgcggg acccagtccg
    22981 ccatagatgt cggcggcgac cagctcgagc ggtgtttccg cgcgcgcgga aaattcggcg
    23041 ggagtcagca gcgcgttctt cgcgcccgag atgatcccgg cgagctgccg tgggggatac
    23101 gccttcggcg agtggccccg ctgctcgagc atgcgcttga tcagcgcctg cgagtcttct
    23161 tcatcgtaaa tcgtgaagtt gggaccgaac cccaggaggg gagcttcccg gcggagcaag
    23221 cgcgccgaga gggagtggaa cgttccgatc cagagcccgc gggggtccgc acccaggagc
    23281 tccgccaccc ggcggcgcat ctcgccggcc gccttgttgg tgaacgtgac ggcgaagatc
    23341 cggtcggcgc ggatcccccg gtccttgatg agatgcgcga tccgcgccgt gagcacgcgc
    23401 gtcttccccg atcccgcgcc cgccagtacc aacaggggtc cgtccccgtg ctcgacggct
    23461 gcgcgctggg ttggattgag cggcggagtc acgaaaggtt acggtaccgg gaagtcgagt
    23521 atgaaacgcg agccgggggt cgccgggtgg tacgacaccc tcccggcgtg ctgctgttcg
    23581 acgatccggc gcgcgagggc gagtcccaga ccccagccac caggcttggt gctgcggccg
    23641 ggctcgaaca tgtgggaacg aatgtgcgcg gcgacgccgg gcccgtcgtc ttcgaccacg
    23701 acgcgccccg tctttcctac cgcctccacg cggacctcga tgcggcctcc gcgtcctgac
    23761 agcgcatcga tggcgttctt caccagcgcc tcaatcgccc actccacgag cacgggatcg
    23821 ccgtcgatca ccgggcccgg gccgatggcg tgaagcacga gctgcaccgg actcgcgagc
    23881 gtcggcagcc tcggccggaa atatcctacc acccgttcgg cggccgctcc caacccgacc
    23941 ggttcgcgcc gtgccggacg cccgatccgc tcgaagcgct tcgcgacccg ctgcagccgc
    24001 tcggtatcgg ccgcgagatg ctccaccagc tcaccaccgg aggttccggg attctcgcgg
    24061 agatagtcga tccatccggt gaggctcatc aacggcgtgc cgagttgatg cgccgactcc
    24121 cgggccatcg cgacccacag ccggtctcgc gccgctccca ccttcgtcgc gttcgcgagc
    24181 cagatcaggc cgatgagaca gagcagcacg gccgcctgca gcagcgccag caccttgagc
    24241 ctccgggcca cgggcaacgc gccgtagtgc acggtgccga tgccgggttg cacgatcggg
    24301 tcgttgacct catccagtcc gcgaacccag gcggcgagcg ccaccgaatc ctgctcaacg
    24361 ccctccggcg cgttcgccac ctgcgtgatg tgcccggtag cgtccgtcac cgccatcggg
    24421 agatgcatgg cgcggacttc cccggcgaga tcgagcagcg cgccggtcgc ggcgtccggc
    24481 tgcggatcgg acaatcccac gaacacacgg ccaaagagcc gggaggtatt ccgggcgtcg
    24541 tcctggaagt gactggcgac cacccatgcg tcggccacgg acagcgcacc gaaaagcaga
    24601 gcggccccga tgagggccgc gggtcctgcc cggcgccacc ggtcctgtct atccaccggc
    24661 ggggccgagt cgatcgacac caacgtacgg cacgagcgcc ttcgggagcc gtacgctccc
    24721 gtcggcttcc tggccatgct ccaggagcgc ggcgatggtc cgcggcagcg cgacccccga
    24781 ggcgttcagc gtgtgcacga aatcgggttt ctcgccgggc ttcgggcgga accggatgtt
    24841 cgcccggcgg gcctggaaat cggtgaacac gctcgcgctc gacacctcga gccacgctcc
    24901 cacgccgggc gcgaacactt cgagatcgta ggtcatggcg ctgccgaagc cggtgtcacc
    24961 ggcggccagg aggacgacgc ggtacggcag ctcgagccgc tcgagcaccg tggccgcgtg
    25021 cctggtgatc tcttccaacg cggccgcgga cgtctcggga gcgacgagac gcaccagctc
    25081 gaccttgtcg aactgatgca cacgcaagag cccccgcgtg tccttccccg ccgcccccgc
    25141 ctcccggcgg aagcacggtg tgtacgccgt gtaggcgagc ggcagcttcc cgccgtcgag
    25201 gatctcatcc cgatggatgt tcgtcaccgg aacttcggcg gtgggaatca aaaaaagatc
    25261 atccggcgcg gtcttgtacg cctcttcttc gaacttcggc agctgcccgg tgccttgcat
    25321 gctctcgcgc ttgaccacga acggcggcgc gagttcacgg taaccatgct ccctggtatg
    25381 caggtcgagc atgaaggaaa tcaggccgcg ggacagcctg gctcccattc ccgtcagcag
    25441 cgggaacccc gagccggaga tcttggcgcc gcgtggcaga tcgaggagac ccagctgctc
    25501 tccgatttcc cagtgcggct tgagtccgct ctcggaccgc ggcgtgcccc aggcgcggac
    25561 gaccttgttg gcgtccgcgc caccgtcggg gacggccccc agcggcagat tcgggacgaa
    25621 cagggccttg gccgaaagca ccgtctcgat gctccggagc tcgtgctcga gggccgtgat
    25681 cttgagccct gcttccttcc gttgatcgat gatttccttc gggagcttgc cgtgctgctt
    25741 caccgccgcc gcgtcttcga gggcgaattt gttgcggtcg cttttcagtg cgtcgagctg
    25801 ccccgagagc gtgcggcgcc gttcatcgag accgcgcagc tcgtccagga gcgccatgac
    25861 cgatgcgtcc ccccgacgag ccagagcggc gcgcacctcg tccggcgagg accgcagccg
    25921 cttcaggtcg atcacggtct aatgcttcgg gtaaccgggc gtgaggtccc gatagccaca
    25981 gttcctgtcg ctcaagatct gaaaggtggt ggtgcgggcc accgtgtcga tagagagaat
    26041 ctgcagcttg gcgtattgga agacggtgcc gatcgcgcac gacacctgcc gggaacggac
    26101 cagcaccacc gtgccggaat cgacgttcag cgcggtgcta tcgttccacc cggggcctgg
    26161 tgccgtggtg gttccgtcga acgagctggc gaactggagc gccgacgtgc gggcgagacc
    26221 ggtgagcgcg ccgggcggat acagcttggc ttgggacccc acgatgtcga aaaggaaatc
    26281 gaagatcggt gtcgtgtcgg tgcgcacccg cgcccccgta acggtgtctt cgatgttgta
    26341 ggccgagggg gtccccacgg gagtcccggt cgccgcgtat agcgtcgcgg tatccaccac
    26401 gttcacgaag ctgggcgcgg gcggggccag cgtgctgctg caggctcccg acaggacccc
    26461 ggcggccagc gcagcaccgg cgcaaagaga cgaaatgggg cgcatgagtg ggtgaagaat
    26521 acccgagcgc ctccaaatgg gtcaacccac ggtggctgct tgacttcgcc cctgcccggt
    26581 cctacgttgg cgctcccaat ggcaaccatc cgtgatgtgg tcgaggcgct tggaaaacgc
    26641 cctggggtag acgctgtcct cgtggtggga cgagatggct tggccatcga cacccacgtg
    26701 gtcaacggcg tcgacccgga aagcgtttcc gcgctcctcc ccggcgtgat cggggccatt
    26761 acccagctgg gctccgcagg cggccgggga gacttcggta ccggcgttct ggagttcggg
    26821 ggtgggctgg cggtcgtttc cgtcctgacg aacgatgccc tcctcgtagt cctggtccgc
    26881 cccgcgacga acgttggccc gctcctggtc gaccttcgcc gccatcgatc cgctatcgcc
    26941 ggtctgttct aaaccgggca tgagtcgggc caactccgcc ccgccaggcc cccttcggcc
    27001 accccggcac ctgctcgtcg tcgacgacga gccgcacatc ggcctgttgc tccgccccga
    27061 gttcgaatcc cgcggctacc gggtgagcat cgcccgctcc ctggctgagg cgcgcaccac
    27121 ggtggactcc gtcgatctga tgctcctcga cgtgcacctc cccgatggat ccgggatcga
    27181 cttcctccgc gagttgcgcg gccggccccg gacgcagaac ctcccgattc tgatcctgac
    27241 gggagaatcc ggggatcggg tgctcgagga agcccgcctc ctcggtgccg gactcctcac
    27301 caagcccttc agtccgacca agctcggcaa gagcatcgcc gcgctgatcg atggtccggc
    27361 tccggtgccg ggggacgccc gatgagcacg tgggcggcca tcctcgccgg tggctcgggg
    27421 acgcggtttt ggcccctgtc caccccggac cgtccgaagc agctgctccc gctcgccggg
    27481 gatcgccccc tgctcgcgca ggcggtgcga cgcctcaccg gcctcgtccc tcccgagcgc
    27541 gtcctcatcc tcacgggccc gtacctcgtg gagcaggtcg cgtccgccgt ccccgaggtg
    27601 ccgcggggac agatcctggc tgagcctcgt accgcctcga ccgcaccggc actggtctgg
    27661 gccgcgcagg agatcgccaa gcgggacccc ggcgccgcca tgctgtcgct gcacgccgat
    27721 tgggcggtgg gtgacgacgc cggcttccgg gcttccgcca ccaaggcgat cgacgtcgct
    27781 caggcacacg acgtgctggt gaccgtcggc gtgaagccga cccgcgacga aaccggttac
    27841 ggatacatcg tgcccggagc gaaactggga aagacccccg cgaagaaagt ccggcgtttc
    27901 gtcgagaagc ccagcgccgc gcgcgcgaag atcctccgca ggggaggcgc gctctggaac
    27961 acgggactct tcgcctggag tacggagcgc ttcctgactg aagcacgtgc ccacgccacc
    28021 gagctgcgcg cgggatggca cgccttggac gacggcgacg ttcagggatt cttctcccgc
    28081 gtcacacccg tcgccgtgga cgtcgcggtg ctggaacgga ccaagcggct cgcgaccctg
    28141 gccggcggat tcacctggga cgacatcggc tcgtgggatg ccctgctccg catccggacg
    28201 cccgacgcgc acggcaacgt ggtggtcggg aacgtctccc tgggggacga cgtgcgccgc
    28261 tccgtgatct ggaccgacga cgagcacctc gcggtcacgg gtgtcacgga catggtagtg
    28321 gtccgcgcca acggccacac gctcgtcatt cccacaggac gcgcggagaa gctgaaagcc
    28381 ctggtgcaaa aactgtgagc accgctcccg ccctctactt gctcgatccc gagcccgccc
    28441 ccgcgtgggc gcccttcatg ggctcgcggc ccatcgccga gctccgggcg ggcgcgcatt
    28501 tgatccgcga gcggtgggaa gcgttcgtgg gcaccgaaac cgcggcggta ttcgccctgc
    28561 cccatctcgc gggattcacg gagcccgggt cggcgcccgt gcgcgcgcgc gaggcggtca
    28621 cgggtcccgc cgtgatcggc tcctcgacgt tcgctccgcg cggcctcgcg cccgccctgc
    28681 ccgatgaacc gtcgcgcctg gtgtgcggag gggtgaccgt cggctggggt gtgcccgccg
    28741 gggcccgctg ggacgggccg gcgcgagagg cccgggccgt cgaggttccg ggcgtcgtcc
    28801 tccatggcgt ccacgggctg ctgcccgtac tcgaagccct gctccgcgaa gatcttcgcg
    28861 gcctgctccc cggaaccgac gggatcccgg ccggctcgac cgtcatcggc gaccccggcc
    28921 tggtcgccgt caccgaaggc ctgatcgaac ccggtgtcgt attcgacacg cggcccgggc
    28981 ccgtggtgct cgagagcggc gtagaagtgc ggtcgggctc ccggttggaa ggcccgctct
    29041 gggtcggcgc caacgcccgc atcctcggcg gctcggtccg ggcgtccgcc atcggcccgc
    29101 gttgcaccgt gcgcggtgag atctccacct gcgtcttcct ggggtacgcc aacaaggcgc
    29161 acgacggctt cctcggccat tcagtggtcg gccgctgggc gaacctcggc gccggaaccg
    29221 tcacgtcgaa cttgaagaac acgtacggac cggtgcggct cgaggtggcg gggcatgaca
    29281 tcgaaaccgg gcagatgaac gtcggctcgc tgatcgggga ccatgccaag accgcgatcg
    29341 gaacgctgct ctcgaccggc acggtgatcg gcaccggcgc gagcgttttc gacgtggtgc
    29401 gcccgccgaa gtacatcccg gcgttcgcct ggggagggac cggagacgcg cggaccaccc
    29461 gggaaggatt tctcaaggtg gccgagcggg tcctgccgcg ccgggacatc gtgatggatg
    29521 aagcgacgcg cgccctgctg ggccgcgtct acgactgggc cacgcggtga gcgacgccga
    29581 gaccagccca caacccgcgc cacccaagca gggagcggca aagcccgacg cggcgtttcg
    29641 ccggctgcac aagttcgagc tgcacgaacg gatcggcgaa ggggcgatgg gcgtggtgtg
    29701 gaaggcctac gactccatcc tgcgccggtt cgtcgccctc aagctcttga acccgtcgtt
    29761 cggccgcacc caggagcttc gtgagcgctt ccaccgcgag gcccgggcgg cggcggcact
    29821 ccagcatccc aacatcgtca ccgtgtacga cctcggtgaa tcggatggcc agctcttcat
    29881 cgcgatggag ctcgtcgaag ggcgcgacct cagcgacctg atcgccgtcc gcgcgcccct
    29941 gcccctcgag cacaagctgg acgtcgtcat cgagctgctg cagggattgg gctacgcgca
    30001 cgcccggggc gtcattcatc gcgacgtgaa gccgtccaac gtccgaatcg ccgcggacgg
    30061 ccgcgtgaag atcatggatt tcggcattgc gcggctccag aacgcggatc gcaccggttc
    30121 gggaggcgtg atcgtcggaa cgccgtcgta catggcgccc gagcagatca cgaacggcga
    30181 gatcacgccg gccaccgacg tgttcgccgt cggctgtctc ctgtacgagc tcctgacgta
    30241 cgagcggccc ttcgaggccg agacggtaca cggcgtgttc taccaggtgc tctcgaccac
    30301 gccgaagccg ctgcggaccg tcgcgccctc catgccggcc tccctcgagc gggtcgtctt
    30361 gaaggccatg gcgaagacac cgggcgaacg ctacggcacc gccgaggagt tccgaagctc
    30421 gctgctcggg atgcggcatg cgctctcgga cccgtcgtcg atcaccacgg agcggatccg
    30481 gttccggaat ccgttcacgc gcatttccct gagcctgatc gcgcccgcct cactcaagtc
    30541 gcgggcgacg gtactgctcc tgctcctcgg ggcggcggtg ttcatcgcgt acctcgccct
    30601 ctcgaccccc gcaccggccc cggcgtccct cccggccgca tccaccgttc cgatcctctc
    30661 accgcgcccc attcccggcc tggcaccccg gatcgcgatc ctgcgcgaca ccgcatatgc
    30721 ggagcgggcg cgagcattgg aggcgggcgg tctcatcaac aacgtccagc ccatgctcaa
    30781 cgccgacgcg atcctgaccg ccgcggacca gtacgcccgc gcgaaggacc tggtccgggc
    30841 gagcacggga tacggctccg tcggccgact gtaccgtcag gcgatcacgg agaccgacag
    30901 cgagcggctg gacgccaaac ggcggttgga ccgcgccgcg acgctggtcc ggtcgatgcg
    30961 ggggaacccg ccgacccgga tctccgcatc gttgactcgt gccgattcgc tcttcaacgc
    31021 gcgggactac gtgatgtcgg ccatcgcttc aggcgcgacc gagcaggccg ccctggccga
    31081 cggcgcgcgg atgcgcaccg taacgccgca gccgcgggat ccgcacaaag ccatcctgca
    31141 attgctggac gatttttcgc gcgcgattca gagccggaag gtgtccaaca tgcgggtgct
    31201 ctacccgtcc attaccaaag gcgaagtgaa gaactgggag gattttttca acggggtcac
    31261 gagcgtcgac gtgaccttca cgcctgactc aataaccatc aaggatccga cggcacgggg
    31321 cgtcgtgcac gcgcacttcc tcgccatcca cggcggctct ccgacgcccc aggagtctca
    31381 gcagaccttc gagacgtcct tccgtcacaa tccgggcggg tggaggatct cggggattct
    31441 ctcccgctga actagtccca ggcgcgcgcg gtatcgggtg gcgcgtcggg cacgaccagt
    31501 cccgcgggac gggctgtagg cggtccgacc tttcgcaaag acaccggctt gtccaggtcg
    31561 ccgcccgaca gcgtgagctt gtcgcccgcg accttcaggc cgtagacctg ccaggcgccg
    31621 tcgcccagag tgaaccagat gctgtctccc accaggcgcc accgggcgac ctgctcgccg
    31681 aattgcgccg aggaatcggc acgcaccatg acgtagtgga ggccgtcgga ggctttcgcc
    31741 tcccactgcc cctccaggtg cacccgtttg gccacctgcg ctccggcgat gacagggagc
    31801 gcgagcgcgc cgacgattcc gagaacgatt ctgttcatgt gggccgttgt ccgccgttag
    31861 tgggtacccc cgtgggcgcc accggtgcct gctcgtcctg cagcaccgcc acgcgaggtt
    31921 cccatttccg catcatcgcg atgaacggcg ccaccacctc gggatcaaac tccatccctg
    31981 cccgcccctc gaggtacggg agaatcttct ccgcctccca ggcgtcccgg taggggcgtt
    32041 tggtccgcag ggcgtcgtag acgtcgcaca cgtggaccag attgctcgcc cggtgacagg
    32101 cccgcccgta atgcggccgg ggatagccgc cgccattcaa catgatgtgg tgctcgtagg
    32161 cgaccacggc cgccagatcg agcttttcct ccgcctcgag gatgatccgg gcaccctggg
    32221 cggggtgcgc gttcatgacc tcccgctcat cgggcgtcag cttcccgggc ttggtgagga
    32281 tctcgagcgg gatccgcgtc ttgccgaggt cgtggagcaa ccccgccacc ccgaacgccc
    32341 gcacgtcctt cgctcccatc cccatgtact ccgccagcgc catcgcgagc accgagacgt
    32401 tcatcgaatg ggtagtcgtg tactgatcga actccttgag gtgcaggagc ggcagcacga
    32461 tctgctggtc cccgtgcatc gccaccgaca gcgaccgcac caccgcctcg gcctccgcca
    32521 ggggcaagcc gccgtgggcc ttcacttcct cgtgcatcca gcggatggct tccgcttcct
    32581 ctccgagcga gaatcggatg gtggcttcga cgggaaggac ggcctgcgga ttccgctgct
    32641 cacccctgat cccgacgctg ccgaacttga tgctcgatcg cttcccctgt tgccgtacct
    32701 cggacgtatc gatcgtcgcg agcgtgagcc gcgcgagcac ctcgtccagg aaggtctcga
    32761 actcctcccg ggtcaccccc ggctggaact cgagccgctg gatccccgcc tgggccagcc
    32821 gcatggacca gtcccatgcc ttgagctcgc ggatcggggt cttgccgtag accacttcgt
    32881 caccgaggaa cgtgaagagg ggcgtggcgg tgtcctgctg caggccgccg agctgctcga
    32941 aggccgcgtc gatcccgcgg tcccgggccg ggtgtccctc cgcgtagagg gacatgccgg
    33001 cgagtgcctg cgccagggag ccgaggaact tgatgggatc gccgctcacg cggttcccaa
    33061 tccgggatcg gtggcggcac ggacatccgc gtcggccgat tgcgcggcgc gcgccaggac
    33121 ggcggtggcc cggtcgtcgg tgccccatcc cgccgcgagc gcctggaggg cggcgagcag
    33181 ctcgggggat ttcgccggca gcttcgccct ccccaggagc gtgcgaccgc cgtcggtgag
    33241 atcgaccagg acgagcaacg cctccggcgc gcccgaacgt ccgagcgcct tgatcgccag
    33301 cacgcggtcc tcggagggca gtgagtcgtt gcgcgcgtgt tgcagcagca gcggcacggt
    33361 ggacggcggg caatcggttt gaatggccat cagcgccatc tgcagcgtct gcgggtcgcg
    33421 atcagcgagc gccgccagca atgcatgttc ccgttccgtc ggatgcttca gctcgagctt
    33481 gagcgcctgc cgcctgacgc gcgcgtccgg gtgcacgcgg aaggcggccg gtgaaaaccc
    33541 ggccggcaag gtcggcaggt cgtccagcag cgcaagcagg ttgcggacga cgtaccacct
    33601 ctggtccttc agccgcggca ggagctccgg cacgaagcct tcgcccatct tgcccaaccg
    33661 gtcgagcagt gcgcggcgca cggcacgcac gtcggcggtc gccagtgcgt cgagcaaggg
    33721 cgccgcggcc gccacgccga cgccctggat gagctggtcg accagcggga agtccggctg
    33781 cggctcggcc agaattctcg cgacggcttc gacgttgaag atgcggttac ggatcgtcgt
    33841 ggccgtggcg ctcccggccg gggcaccttc cagcagctcg agcaggcgtg agaactggtc
    33901 ccccgacacc atccggtcca ccgcgcccca cacgatgggg cccgtggcgt cgatctcgag
    33961 cgccatctcc accagccgtt cctcttcggg ggcgaagacg gcatcgggcg ccgtcgcgaa
    34021 gatcggcccc gccctcgaca tctcctgcag cgccttccca taggcgccgg ggttggggtc
    34081 gtccagttgc cagccggaca tcagctgctc cacctggtca cggagcgcgg cgtccgcgag
    34141 gggacgcgcg agcgcggacc cctgctccgc gtgcgcggcg agcttggaca gcatccggac
    34201 cagcgagtgc gacatggtct gatgggagga ggtcgccgcg gcccggacga tttcgatcac
    34261 ggcgtcgacc gccatcccat gcgtcgcatc gagcacgaac ttgtggcgtt gggcgaagtt
    34321 cccacccatg tccacgagcc gctggagcgt ttccggccgg agctccgcga tcatgcggga
    34381 ggtccgcttc cggagctcca tcgcttccct gccgcccgcc gtcttgagct cctccgcgat
    34441 ttggaggaga tacccgacga tcacctgatc gtaggcggcc ccccgttcgt gggcgttgat
    34501 ggcctccgcg accaccgcct ccttgagctc cgatgccgcg tcgtcgtcct cgactccccc
    34561 cgccagcgcc gctcgcgcca gtccgatcca cagctgggcg ctcttcgccc gctcggcctt
    34621 cgacccgacc ggattgccgt cgtcatccag cagctcgagc ttgtcgtacg tcatcgggtg
    34681 cagcttgacg tggtcccaat gcgggatcag gtcccggggc ccgagcccga tggcctccac
    34741 tccccgcccc ggctcgcttg acagcatccg gagcgcctcc tccagctcac caccctcgat
    34801 gccccgggcg aaggagacgg cgcccaggtg atggcggtgg aggcgtccgg cgag
//
LOCUS       DASUBP010000206         8160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_905574, whole genome shotgun sequence.
ACCESSION   DASUBP010000206 DASUBP010000000
VERSION     DASUBP010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 8160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8160
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905574"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            33..587
                     /locus_tag="VFU75_07915"
     CDS             33..587
                     /locus_tag="VFU75_07915"
                     /inference="COORDINATES: protein motif:HMM:NF014845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V4R domain-containing protein"
                     /protein_id="HEU5466801.1"
                     /translation="MSPSSASARTDGLAIGRASLQQLHRSLLQHAPDQAVTVLQETGY
                     ASGEGIYQAFCAWLPGAAGVSKPEDLDTAHVNSVLAEFFRARGWGTVTVQPVGGAALA
                     VDSTDWVEADPGSAEAPMCFFSAGMLADFLGRVSGEPVAVMEVECRSKNDARCRFLSA
                     APETLQHVYEQMTAGRTYEDALTG"
     gene            716..895
                     /locus_tag="VFU75_07920"
     CDS             716..895
                     /locus_tag="VFU75_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466802.1"
                     /translation="MLTLNLNADQAALLTRILDLYFAELKKAGASREVAPRFAAEGPL
                     LRDLLVQLAAYQHAA"
     gene            complement(974..2299)
                     /locus_tag="VFU75_07925"
     CDS             complement(974..2299)
                     /locus_tag="VFU75_07925"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEU5466803.1"
                     /translation="MIPKALDALKRAADAGDACWRRSERTTIAFESGRLKSAGVVEES
                     GLNVRVLKDGKVGVAGTTATDAPPSDVVTRALASAALGEPLAIEFPSANRFPSVATEA
                     KSAGAASVGALADIGRGLVERLSRPDAQINVTVEREVAETTIGNTAGGQGSYRATGVS
                     VSADIARVSDNDVLMIYDYYAGVGMPDAADLDAVVESIKTRLELALTIVASPSGSLPV
                     VFSPMGLSAILLPLEEALSGKTLIQGISPLQGKVGEAVFAEALSITDDPTLANRPASY
                     PFDDEGVPAAPLPLIERGAPRHFVHDLETAARLGGAQRSTGHGHRGVFGKPGLSYSNF
                     IVGTTPTARPRWSLGGGLTDGIRDGLLIDDLIGVGQGNVIGGAFSHPVALAYRVQNGK
                     ITGRVKDAAVAGNVYDLLKTIGGWGNDGRWVGTRWSPSLLLEGVSVAGR"
     gene            complement(2296..3672)
                     /locus_tag="VFU75_07930"
     CDS             complement(2296..3672)
                     /locus_tag="VFU75_07930"
                     /inference="COORDINATES: protein motif:HMM:NF039907.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HEU5466804.1"
                     /translation="MRDVLDAAIRASRADYTEIRVERTWATGVSFRGARLESATASED
                     VGGCVRVLSKGHGWGVAAFTSLNQLPAMIERAQELSRAIVLDEPIRLADVSPQTAEAL
                     LDLDGDVRGISLAEKTRLIEAYNGEMLAVSGDITDTIANYHDEVTEYWFVNSEGTSLH
                     ELRPEVTLSGTAIARKNGTIEKALESIGLRKGWNSVHTFAEGFKKAGERASQLLRAPT
                     VRGGNYPVVLDPEMAGVFIHEAFGHLSEADFVYENPQAREMMTLGRRFGRKVLNVGDN
                     GAVPGLRGTMPFDDEGVPTQDSVLIKEGELVGRLHSRETAARMGERPTGNARALSFRH
                     PPIVRMTNTYIGNGEGTFEDLIKDVKLGVYACSAFGGQTMLESFSFTAAYGYMIRDGH
                     VAELVKDVVLAGNLFQTLDRIERIAGDFQWNQIGGGCGKAGQAYLPVTEGAPHVRIED
                     ALVGGEVG"
     gene            complement(3676..4155)
                     /locus_tag="VFU75_07935"
     CDS             complement(3676..4155)
                     /locus_tag="VFU75_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HEU5466805.1"
                     /translation="MLEVDWPFFGELCRVLALKVFRAYDPELVLGIARAGVIPGAVVA
                     SIMQRDFASMAITRSESGARPVVIAGPPRLVTGRRVLIVDETCDTGDTMKLALNAVRE
                     LRPASVKTAVSFRTGPYQVDFSALDTDSFIILPWDREIIQNGEIVVRPDYADKLRGV"
     gene            4283..4729
                     /locus_tag="VFU75_07940"
     CDS             4283..4729
                     /locus_tag="VFU75_07940"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5466806.1"
                     /translation="MSFWRRLFGDSSPDEIAPQRLDYLNEALALERQGDFEAALTSYR
                     LALRDHPNDARILQNMAIAFTKTGRVDEAIRHYRRSLELDKSLAGAHYGLAFLLLKRG
                     DPDGAVRHLKEFLAQPPKGPDAQKWIEHAKEQLREMGEAHSPMEAH"
     gene            4768..5439
                     /locus_tag="VFU75_07945"
     CDS             4768..5439
                     /locus_tag="VFU75_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466807.1"
                     /translation="MGVLALACGNISTDLNEVIALRVNLGPDTGKMDFGDTVTLTAVA
                     LNGRGQAVTAPIIWSDLDTNVHIDSLTGAVTAHNIGTGLIQARTGNLRSNPQSITVFI
                     RPDTAFADSTLARDSVSIGTKTDSLSDSIRVRVRHYDPTDTTSLSLRPVLFSLLYPAD
                     TTSFKLFPAGTVLTDVNGLAVVQVRLKQRTLPDSAVVQASVVRYFNHVAVPGSPMTFV
                     VRFYP"
     gene            5436..7253
                     /locus_tag="VFU75_07950"
     CDS             5436..7253
                     /locus_tag="VFU75_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HEU5466808.1"
                     /translation="MSPPQPGTLTQLFFDGLDRFIPGNRPAFKAKRGNAWQPLTHRET
                     EARVRAISLGLKELGVQPGERVALLSETRLEWALVDYACLCAHATDVPIYPTLLVKQV
                     EYILKDCGATTVFCSTAEQVAKVNEIKAHLPIKHVITFDAGPSAGVITLDELEKKGKA
                     ADAKYPNWRADALKVSPDSLATLIYTSGTTGDPKGVMLSHDNLYSNVSACVHILPISE
                     SDECLSMLPLSHVYERMVDYTMWMAGVVISYAESFDKVAQNLRETKPTIVLSVPRLYE
                     KVYAKVIEGASKSPLKHAIFNWSKKVGDRWATHRLSGVKIPLGLSIAHGIADKLVFKK
                     VRAATGGRLKTFISGSAPLSADVALFFFACNLPILEGYGLTETSPVLTLNPPQKPKLG
                     TVGTAIPGVTIKIAADGEILAKGPNIMQGYYNQPEETKETLADGWFHTGDIGELDSEG
                     YLKITDRKKDMIKTAGGKWVAPQPIENAVKANKYVASVVLLGDKHKFPLLLVVPNFDQ
                     LEAWAKYRRIAYKSREQLVTLPDVRAKMERECMGMLNDLPRYEMPKKVALLAHDFTIE
                     SGELTPTLKVKRRVVEEHYEKVISAAYAEADPLAAAIER"
     gene            complement(7287..7883)
                     /locus_tag="VFU75_07955"
     CDS             complement(7287..7883)
                     /locus_tag="VFU75_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466809.1"
                     /translation="MHIELTEMLRCPEKHQEEFLVLSTVEMSGRMVARGLLGCPVCHK
                     EYEIANGVVEFGGISTHHAPPFSTADPVTVQALLDLSGPGGYVILVGSAIRYAVGLAS
                     LMGGIHFVGINPPLDAEELPILSLLRAENVIPLKSSIARGVVVGPEVATSKWLSEAKR
                     ILLKGRRLLIESEQVPEIAGVNRLAVGQGLWVGEKLEG"
     gene            complement(7873..>8160)
                     /locus_tag="VFU75_07960"
     CDS             complement(7873..>8160)
                     /locus_tag="VFU75_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466810.1"
                     /translation="GVVAGQVWDSKGQPVQQARIYGLVKPEPQETPLSYVETYGPRNH
                     QDPAYHEHFAIGDVPKGEYWLGVEIEGKKVYRNIIVQPGKLTWVEFRPDAH"
BASE COUNT         1430 a         2745 c         2604 g         1381 t
ORIGIN      
        1 tccagtcaaa cctgtaagtt gggctcccag atatgagccc ttcctccgcc tccgcccgca
       61 ccgacggcct tgccatcggg cgggcgtctt tgcaacaact gcatcggtcc ctgcttcagc
      121 acgcgcccga ccaggcggtg acggtgctcc aggagaccgg ctacgcgtcc ggcgaaggga
      181 tctaccaggc cttctgcgcg tggctccccg gcgcggcggg cgtatcgaag ccggaggacc
      241 tggacaccgc ccatgtgaac tcggtgctcg ccgagttctt ccgggcccgc ggctggggca
      301 cggtgaccgt gcagcccgtc ggcggcgcgg cactcgccgt ggactcgacc gactgggtcg
      361 aggccgatcc cggctccgcc gaagcaccga tgtgcttttt ctccgcgggc atgctggcgg
      421 acttcctggg gcgcgtgtcg ggtgagcccg tcgcggtcat ggaagtcgag tgccggtcga
      481 agaacgatgc gcgctgccgt tttctctccg cagcacccga aacgttgcag catgtgtatg
      541 agcagatgac cgcaggccgt acctacgagg acgcactcac cggataaagc gggattgggg
      601 attggagatt ggggattcgg aacaccaatc ccgaatccct catcccccat ccccgtcttt
      661 ggaacggaac ctgctctcgg ctcagggtgt taccctgatg cgagaggagg tacccatgct
      721 gaccctcaat ctcaacgccg atcaagccgc gttactgacg cgaattctgg atttgtattt
      781 cgcggaattg aagaaggcgg gagcctcacg cgaggtcgcc ccacggttcg ccgcggaggg
      841 accgctgctg cgggatctgc tggtgcagct cgccgcgtat cagcacgcag cataagaacg
      901 cgggaaacgg aaatcgtgaa acgggaaacg tgatagcaaa cacgtttccc gtttctcgtg
      961 tcccgtttcc cgtttaccgc cccgccaccg acactccctc gagcagcagc gacggcgacc
     1021 accgggtccc cacccagcgg ccgtcgttcc cccagccgcc gattgtcttg agcaggtcgt
     1081 agacattccc cgccaccgcc gcgtccttca cccgtcccgt gatcttgccg ttctgcacgc
     1141 gataggcgag cgccacggga tgactgaagg ccccgccgat gacgttgccc tgcccgacgc
     1201 cgatcaggtc gtcgatcagg aggccatccc ggatgccgtc cgtcagtccg ccaccgagtg
     1261 accaacgggg ccgcgccgtc ggcgtggtgc ccacgatgaa gttcgagtag gacagacccg
     1321 gctttccgaa aaccccgcgg tgcccgtggc ccgtgctgcg ctgggctccg ccaagccggg
     1381 ccgcggtctc gagatcgtgg acgaagtgcc ggggtgcgcc ccgctcgatg agcggcaggg
     1441 gggccgcggg gacgccttcg tcgtcgaacg gataggaggc gggacggttc gcgagcgtgg
     1501 ggtcgtcggt gatcgacagc gcctccgcga aaacggcctc ccccactttc ccctgcagcg
     1561 gcgagatccc ctgaatcaag gtcttgccgg aaagcgcctc ctccagcggc agaaggatgg
     1621 cggagagccc cataggcgag aacacgacgg gcagcgatcc ggagggcgag gccacgatgg
     1681 tcagagccag ctccagccgg gtcttgatcg attcgaccac ggcgtccaga tcggctgcat
     1741 ccggcatgcc gacgccggcg tagtaatcgt agatcatcag cacgtcgttg tcggacacgc
     1801 gggcgatgtc ggcgctgaca ctcacaccgg tggcgcgata gctcccctgc ccgcccgcgg
     1861 tgttcccgat ggtcgtctcc gccacttccc gttcgacggt gacgttgatc tgcgcgtcgg
     1921 gacgcgagag gcgttccacc agcccgcggc cgatgtcggc gagcgcgccg accgacgctg
     1981 cgcccgcgga cttcgcctcc gtcgctacgg aggggaatct gttcgccgac gggaactcga
     2041 tcgcgagcgg ctcaccgaga gcagccgagg cgagggcgcg ggtcaccacg tcggaggggg
     2101 gcgcgtcggt ggcggtcgtc ccggccacgc ccaccttccc gtccttcagg acgcggacgt
     2161 tcagacccga ctcctcgact acgccggcgc tcttgagacg ccccgactcg aaggcgatcg
     2221 tcgtgcgctc actccggcgc caacaggcat ctccggcatc ggcggcgcgc ttcagggcgt
     2281 ccagggcttt cgggatcacc cgacctcgcc ccccaccagc gcatcttcga tgcgcacgtg
     2341 cggcgcgcct tcagtcaccg gcaggtaggc ctgtcccgcc ttgccgcacc ccccgccgat
     2401 ctgattccat tggaagtccc cggcgatccg ctcgatgcga tccagcgtct ggaacaggtt
     2461 cccggccagc acgacatcct tcaccagctc ggccacgtgg ccgtcgcgga tcatgtaacc
     2521 gtacgcggcg gtgaacgaga agctctcgag catcgtctgg ccgccgaacg ccgagcaggc
     2581 gtagacgcca agtttcacgt ccttgatcag atcttcgaag gtcccctcac cgttcccgat
     2641 gtaggtgttc gtcatccgga cgatcggggg atggcggaag gaaagggccc gggcatttcc
     2701 ggtcgggcgt tcccccatac gggcggccgt ctcacgggag tgcagtctcc cgaccagctc
     2761 accttccttg ataaggaccg agtcctgggt gggcacccct tcgtcatcga atggcatggt
     2821 gccccgcagt cccggcaccg ccccgttgtc cccgacgttc aggaccttcc ggccgaagcg
     2881 gcgtccaagg gtcatcattt cccgcgcctg cgggttctcg tacacgaagt cggcctcgga
     2941 gagatgtccg aacgcctcgt ggatgaacac cccggccatt tcgggatcga ggaccacggg
     3001 gtaattcccg ccgcggaccg tcggcgcgcg caggagctgg gacgcccgtt ccccggcctt
     3061 cttgaacccc tcggcgaacg tgtgaacgct gttccagccc ttccgcagac cgatcgattc
     3121 gagcgccttc tcgatggtgc cgttcttccg ggcgatcgcc gtccccgaga gagtgacctc
     3181 cgggcggagc tcgtgcaggc tggtcccctc cgagttcacg aaccagtact cggtgacctc
     3241 gtcgtggtag ttggcgatgg tgtcggtgat gtcccccgag acggcgagca tttcgccgtt
     3301 gtaggcctcg atgagacgcg tcttttccgc gagggagatc ccccggacgt caccgtccag
     3361 gtcgaggagg gcttcggcag tctggggaga cacgtccgcc aggcgaatgg gctcgtcgag
     3421 cacgatcgcc cgggacagct cctgggcccg ctcgatcatg gcggggagct ggttcagcga
     3481 cgtgaacgcc gcgacgcccc agccgtggcc cttcgagagc acgcgaacac agccaccgac
     3541 gtcttcggac gcggtcgccg attcgagtcg agcccctcga aacgacaccc cggtggccca
     3601 ggtgcgttcg acgcggattt cggtgtagtc ggcacgagac gcccggatgg cggcgtcgag
     3661 aacatccctc accagctaca ccccgcggag tttgtcggcg tagtcggggc ggaccacgat
     3721 ctccccgttc tggatgatct cgcggtccca gggaaggatg atgaagctgt cggtgtcgag
     3781 cgcggagaaa tcgacctggt agggcccggt ccggaacgaa accgccgttt tcacggacgc
     3841 ggggcgcagt tcccgcacgg cgttgagggc cagcttcatg gtgtccccgg tgtcgcacgt
     3901 ctcgtccacg atcaggaccc gccggcccgt gacgaggcgc ggcggaccgg cgatcaccac
     3961 cggacgggcg cccgattcgg agcgggtgat ggccatcgag gcgaagtcgc gctgcatgat
     4021 gctcgcgacc acggctccgg gaatcactcc cgcccgggcg atgccgagga ccaattcggg
     4081 gtcgtaggcg cggaagacct tgagggcgag gacccggcag agctcaccga aaaacggcca
     4141 gtccacctcc agcaccgcgt tgggggacgc acgtccggga cgcgaacggc gagggactgg
     4201 gggggtagga ggaccaggag ggacgtacgt cattcgggaa gctacattgc gaatgcaagc
     4261 agccgcaagc tattttcgca tcatgagttt ctggcgccgc ctcttcggcg actcgtcacc
     4321 cgacgagatc gcgccgcagc gactcgatta cctgaacgag gcgcttgcgc tcgaacgtca
     4381 aggtgacttc gaggccgcgc tcacatccta ccgcctcgcg ctgcgcgacc accccaacga
     4441 cgcccgtatc ctccagaaca tggccatcgc gttcacgaag acgggacgcg tggatgaggc
     4501 catccgccac taccgtcgct cgctcgagct cgacaagtcg ctcgcgggcg cacactacgg
     4561 actcgctttt cttctcctga agcgcggcga tccggacggc gccgtgcgcc acctgaaaga
     4621 gttcctcgcc cagcctccga agggtcccga cgcgcagaag tggatcgagc acgccaaaga
     4681 gcagctccgg gaaatgggtg aggcccactc cccgatggag gcgcactgag gcttcggacc
     4741 cgctgggccc tgctcctcgc gggcggggtg ggggtcctcg cgctggcgtg cggcaacatc
     4801 agtacggacc tcaacgaggt catcgcgctc cgggtgaacc tcggccccga cacggggaag
     4861 atggacttcg gcgacaccgt gacgcttacc gcagtcgccc tcaatggccg cgggcaggcg
     4921 gtgacggcac ccatcatctg gtccgatctc gacaccaacg ttcacatcga cagcctgacc
     4981 ggagcagtca ccgctcacaa catcggtacc gggctcatcc aggcgcgcac cggcaacctc
     5041 cgctccaacc cgcaatccat cacggtcttc attcgaccgg acaccgcctt cgcggacagc
     5101 accctcgcgc gtgactcggt gagcatcggg acgaagaccg attcgctctc ggactcgatc
     5161 cgggtgcggg tccggcacta cgaccccacg gacaccacct cgttgtcgct gcgcccggtc
     5221 ttgttttccc tgctctatcc ggcagacacc acgagtttca agttgtttcc cgccggtacc
     5281 gtcctcacgg atgtgaatgg actggcggtc gtgcaggtga ggctgaagca gcgtaccttg
     5341 cctgattcag ctgtggtcca ggcgtcggtg gtacgctact tcaatcacgt ggccgtgcct
     5401 ggatctccca tgaccttcgt ggtgaggttc tatccatgag ccccccgcaa cctggcacgc
     5461 tgacccagct cttcttcgac ggactggacc ggttcattcc ggggaaccgg cccgccttca
     5521 aggccaagcg cgggaatgcc tggcagccgc tgactcaccg ggaaaccgaa gcgcgcgtca
     5581 gggccatttc cctcggcctc aaggagctgg gggttcagcc gggagagcgg gtcgcactgc
     5641 tctcggaaac ccggctggaa tgggccctgg tggactacgc ctgcctctgc gcccacgcca
     5701 ccgacgttcc gatctacccc accctgctgg tcaaacaagt cgagtacatc ctgaaggact
     5761 gcggggccac gacggttttc tgttcgacgg ccgagcaggt ggcgaaggtc aatgagatca
     5821 aggcgcacct gcccatcaag cacgtcatca cgttcgacgc cgggccgagt gccggtgtca
     5881 tcacgctcga cgagctggag aagaagggca aggcggcgga cgcgaagtac ccgaactggc
     5941 gggccgacgc tctgaaggtc tccccggatt ccctggcgac cctgatctac acgtccggga
     6001 ccacgggtga ccccaagggc gtgatgctca gtcatgacaa cctctacagc aacgtctccg
     6061 cctgcgtcca catcctgccg atctccgaat ccgacgagtg cctctccatg ctcccgctct
     6121 cgcacgtcta cgagcggatg gtggattaca ccatgtggat ggcgggtgtg gtgatcagtt
     6181 acgccgagtc gttcgacaag gtggcgcaga atctccggga gacgaagccg accatcgtgc
     6241 tgtcggtccc gcggctctac gagaaggtct atgcgaaggt gatcgagggc gcctcgaaga
     6301 gcccgttgaa gcacgcgatc ttcaactggt ccaagaaggt gggtgaccgc tgggccaccc
     6361 accgcctctc gggcgtgaag atccccctcg gcctgtccat cgcccacggg atcgccgaca
     6421 agctggtctt caaaaaggtc cgagccgcca ccggcgggcg gctcaagacg ttcatctccg
     6481 gcagcgcgcc cctctcggcg gacgtggcac tcttcttctt cgcctgcaac ctgcccattc
     6541 tcgagggata cggcttgacc gaaacctccc ccgtcctgac cctcaacccg ccgcagaagc
     6601 ccaagctcgg caccgtcggc acggcgattc cgggcgtcac catcaagatc gccgccgacg
     6661 gagagatcct ggccaagggt ccgaacatca tgcagggcta ttacaaccag ccggaggaga
     6721 cgaaggaaac gctcgccgac ggctggttcc acaccgggga catcggcgag ctggactccg
     6781 aggggtacct caagatcacc gaccgcaaga aggacatgat caagacggcg ggcgggaagt
     6841 gggtggcgcc gcagcccatc gagaatgcag tcaaggccaa caagtacgtc gccagcgtgg
     6901 tgctgctcgg ggacaagcac aagttccccc tgctcctcgt cgtgccgaac ttcgaccagc
     6961 tcgaggcgtg ggcgaagtac cgccgcatcg cctacaagtc acgggagcaa ctggtcacgc
     7021 tgccggacgt gagggccaag atggagcggg agtgcatggg gatgctcaac gacctgccga
     7081 gatacgagat gccgaagaag gtggccctgc tcgcacatga cttcaccatc gaatcgggcg
     7141 agctgacgcc gaccttgaag gtgaagcggc gggtggtgga ggagcactac gagaaggtga
     7201 tctccgcggc ctacgccgag gcggacccgc tggccgccgc cattgaaagg tagaactagc
     7261 cgtctagccg tctaaccgtc tagtttttag ccctctagtt tttctccgac ccagagtccc
     7321 tggccgaccg ccagccgatt cacaccggcg atctccggca cctgctcgct ctcgatcagc
     7381 aggcgccgtc ccttcagcaa gatgcgcttt gcctccgaca gccatttcga ggtcgccact
     7441 tcgggcccca cgaccacgcc ccgggcgatg gagctcttca ggggaatcac gttttccgcg
     7501 cgcagcagcg acaggatggg gagctcttcc gcgtcgaggg gtgggttgat gcccacgaag
     7561 tggatcccgc ccatgagtga ggcgaggccc acggcgtacc ggatagcgga gccgacgagg
     7621 atgacgtagc cgccgggtcc ggagaggtcg aggagggcct gcacggtcac tggatcggcc
     7681 gtggagaagg gtggcgcgtg atgcgtggag atcccgccga actccaccac cccgttcgcg
     7741 atctcgtatt ccttgtggca gacggggcag ccgaggaggc cgcgcgcgac catccgtccg
     7801 gacatctcga cggtggagag caccaggaac tcttcctggt gtttctcggg gcagcggagc
     7861 atctcggtca gttcaatgtg catcggggcg aaattctacc caggtgagct tgccgggttg
     7921 cacgatgatg ttccggtaaa ccttcttccc ttcgatttcc acacccaacc agtattcgcc
     7981 tttcggcacg tccccgatgg caaagtgttc gtggtaggcc gggtcctggt ggttcctggg
     8041 gccgtaggtc tcgacgtacg acagtggagt ctcctggggc tcgggcttca cgaggccgta
     8101 gatcctggcc tgctgaaccg gctggccctt cgagtcccac acctgccccg cgaccacgcc
//
LOCUS       DASUBP010000207         2110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_74299, whole genome shotgun sequence.
ACCESSION   DASUBP010000207 DASUBP010000000
VERSION     DASUBP010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74299"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..994
                     /locus_tag="VFU75_07965"
     CDS             <1..994
                     /locus_tag="VFU75_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466811.1"
                     /translation="HGVAQLDSAKAKDLATARSLVKLPALDATQIGAAVFGPAAAGKF
                     EKALHWVRVAREYAPAGLTPHSHPAPKRVRRAGTTVLFPREHDLPAFWLKTGDVSFTL
                     DSAKNYSGKLADLTSSQALTGKPTTFNAQAPLFQAGGLLDHRRPQALDTVSGVSPSVK
                     LPDLAIPVGGIHLAPGTGSMAFRFALEGENLRAAWEVKSSHVTWTHDSAATSGAGGLV
                     WQVVSGISTLDISAQLSGTPDNPSLAIRSNLDQAISDRVKGLMGDQVKAAEQKLEAQV
                     NAYADPLIAPVKSQVTSVTATAQQQLSASQGKIADAQKQLQDRLKSLTGGFRLP"
     gene            1138..1620
                     /locus_tag="VFU75_07970"
     CDS             1138..1620
                     /locus_tag="VFU75_07970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU5466812.1"
                     /translation="MYRAEVGRVYALCLRLTGNRRDAEERTQDVFVQAWRRLSTFRGD
                     SALSTWLHRLTVNLVLTERRGTRRREQRVVEVESLEAVEKPGVGSPKGQVGESFASGV
                     DLERAIAKLPEGAREVFVLHDIEGYTHDEIANACGIAEGTSKAQLFRARRLLREMLER
                     "
     gene            1617..>2110
                     /locus_tag="VFU75_07975"
     CDS             1617..>2110
                     /locus_tag="VFU75_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466813.1"
                     /translation="MSDFEPPIRGVEDLPRSIEPPRDLWPGVRRRIHWRRRSMIGGAG
                     LAAAAALVFFLFPRGPLVPTTVVAGHPSISRVRVVTDDSSRARLTLSIGDVLVEPQSD
                     LRVLTIEPGEQRFELRQGTISARVLAPPRVFVVETPAAVVTDLGCAYVLQVDSLGNGV
                     LRVTA"
BASE COUNT          327 a          691 c          752 g          340 t
ORIGIN      
        1 gcacggcgtc gcccagctcg actccgcgaa ggcgaaggac ctcgcgacgg cgcgcagcct
       61 cgtgaagctc ccggcgctcg atgccacgca gatcggcgcg gcggtcttcg gccccgcggc
      121 agcggggaaa ttcgagaagg ccctgcattg ggtgcgggtc gctcgggagt acgcgcccgc
      181 gggactcacg ccccactcgc atcccgcgcc gaagcgcgtg cggcgcgccg ggacgacggt
      241 gctgttcccg cgggagcatg acctgcccgc cttctggctc aagaccggcg acgtgtcgtt
      301 cacgctggat agcgcgaaga actacagcgg gaagctggcg gacctgactt cgtcgcaggc
      361 actgacgggc aagccgacga ccttcaacgc gcaggcgccg ctattccagg ccggcgggct
      421 cctcgatcac cgccggccgc aagccctcga cacggtgtcc ggcgtgagtc cctcggtgaa
      481 gcttcccgat ctggcgattc cggtcggcgg aattcacctt gcccccggca ccggcagcat
      541 ggcgttccgg tttgcgctgg agggggagaa cctccgcgcc gcctgggagg tgaagtcttc
      601 ccacgtgacc tggacgcacg actcggcggc gacctccggg gcgggcgggc tggtctggca
      661 ggtcgtctcc gggatctcga cactcgacat ctccgcccag ctgtccggca cgcccgacaa
      721 cccgtccctc gccatccggt cgaacctgga tcaggcgatt tcggaccgcg tgaagggact
      781 gatgggggat caagtgaagg cggcggagca gaagctggag gcgcaggtga atgcctacgc
      841 cgaccctctc atcgccccgg tcaagtcgca ggtgacgtcc gtcacggcga ccgcccagca
      901 gcagctgagt gcgtcgcagg gcaagatcgc ggacgctcag aagcagctgc aggatcggct
      961 gaagtccctc acgggcggct tcaggctgcc gtaactgcag gggtcaggtg tcaggggcca
     1021 gtcaaaccct ttcgcctcct tcggcatccc atcgacatca cccgtccgac gcctgaatca
     1081 gatgacgccg ataccctcgc ccgagctcgg gcgggggatg aaacggcctt ccacgtcctg
     1141 taccgggcgg aggtcggccg tgtctacgcg ttgtgtctgc ggctgactgg gaaccggcgt
     1201 gacgcggagg agcggacgca ggatgtattc gtacaggcct ggagacggct gtccacgttt
     1261 cgcggagaca gcgcgctgtc tacctggctg caccggctga ccgtgaatct ggtactcacc
     1321 gagcggcgcg gcacccgtcg acgtgaacag cgggtggtgg aggtggagtc cctggaggcc
     1381 gtggaaaaac cgggagtcgg gagccctaaa ggacaggtcg gggagtcgtt cgcctccggc
     1441 gtggacttgg agcgggctat cgcgaagctg cctgaaggtg cgcgggaggt gttcgtgctg
     1501 cacgacatcg aggggtacac gcacgacgag atcgcgaacg cgtgcggcat agccgaagga
     1561 acgtccaagg ctcagctctt ccgggcgcgc cggctgttgc gggagatgct cgagcgatga
     1621 gcgatttcga gcctcccatc aggggtgtcg aggacttgcc gcggtcgatc gagccgcccc
     1681 gcgacctctg gcccggcgtg cgacggcgca tccattggcg gcgccgctcg atgatcgggg
     1741 gagcgggact cgccgccgcc gcggcactgg tgttcttcct gtttccgcgt gggcctttgg
     1801 tgcccacgac ggtggtcgcg ggccacccga gcatttcgag agtccgcgtc gtaacggatg
     1861 attcgtcgcg ggccaggctg acgctctcca tcggggacgt cctcgtggag ccgcagagcg
     1921 acctccgcgt cctcacgatc gagccggggg agcagcgctt cgagcttcgc cagggcacca
     1981 tttccgcccg ggtgctggcg ccgccgcggg tgttcgtcgt ggagacgccg gctgccgtcg
     2041 ttaccgacct gggctgcgcc tacgttctgc aggtcgactc gctgggcaac ggagtgcttc
     2101 gggtgacggc
//
LOCUS       DASUBP010000208        21772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_210066, whole genome shotgun sequence.
ACCESSION   DASUBP010000208 DASUBP010000000
VERSION     DASUBP010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 21772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21772
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_210066"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..840)
                     /locus_tag="VFU75_07980"
     CDS             complement(7..840)
                     /locus_tag="VFU75_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5466814.1"
                     /translation="MANLSLAAISLGLRGAQAVSPELAGWWAERLFFTPPHRPLSTGD
                     RDALGRARAFTVRSEGHEIRAWRWGTGPMVFLVHGWGGAGGRFSSFVDPILARGFSAV
                     TYDGPGHGLTGRGMSSAPEMARALDAVMAEVGAPDAIIAHSFGGVVTALAMSKGFEPR
                     RVALIAPAAFPLRFVDDFVRALRLSERTGAALRSRSERRIRFDWDQLNVLPVFAGARS
                     PALVVHDQQDETIPVDEGREIAATWPGAELMLTQGLGHRGVLRDPSVVSRAVTFVTNR
                     S"
     gene            869..1966
                     /locus_tag="VFU75_07985"
     CDS             869..1966
                     /locus_tag="VFU75_07985"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5466815.1"
                     /translation="MDTDVLVIGAGPGGLAASQQLKRHGVPHKVLERGDTVGHTWANL
                     YDSLVLHTGKRMSSLPGLAFPADYPTFVSRADFLKYLLQYRETFDLPVETGKPVSRLE
                     RTAAGWTAHVNGDRISCRSLVMATGIVANPVAPRFPGQDRFKGHVMHSVEYRRPDPFR
                     DQRVLVVGVGNSGGEIGAELAHQGIPVTLAVRSGANVVPRELLGIPIQYVAYYVRKLP
                     RPVQRIIVSLVRQITILRRGPPVLPPSPVFALDAVPLIGFHLVDAIKAGTAQVRPGLQ
                     EFTANGVRFTDGREESFDTVILATGFHAALAPLGALVTTDAKGFARRADRVTSADQEG
                     LFFVGSNYDSGGGLLNIGIDARLVGRILAQS"
     gene            2029..4134
                     /locus_tag="VFU75_07990"
     CDS             2029..4134
                     /locus_tag="VFU75_07990"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HEU5466816.1"
                     /translation="MTILDSVLKPASIAVIGASRTPNTIGHQIMANLLRYGYTGPVYP
                     VNPKASSVHSVRAAPSIRALSEPVDLGVIVVPKEQVLEVAAECAEAKVRGLVVISAGF
                     REIGGAGAERERRLLDIVRSNRMRMVGPNCMGVLNADPAFSMNCTFAPTMPPFGHVAF
                     VSQSGALGVSVLDYTKEYGIGIAQFVSMGNKPDVSGNDLLLQWEHDEAVRAILMYVEN
                     FGNPQRFLEIASRITRRKPIIVVKSGRSRVGARAAASHTGALAASDAAVDALLTQAGV
                     LRADSIEELFDMAMAFGAQPLPRSRRTAVLTNSGGPGILAADALERHGLEVTELDAAT
                     VSRLRPLFPEEASIRNPLDMIASATPEGYRTALDAMLDDPKVDSAVAIFVPPLGLSQG
                     AVAEAIVAAAAKHPTKPVLGVLMGREGLPQGKAELHRAGIPAYIFPESAARALAALRR
                     HREWQARPVATEAPLTVDREAARNILCAARAEGRARLDEIESIHLLAAYGIPTTCTRF
                     ATDPNDAGQVAGEIGFPIVLKVVSPDIVHKSDVGGVRVGLATPMEVQIAAAQMLEDVR
                     HRAPDATVRGFVIQRMISGGRETIAGLTRDPAFGPLVMFGLGGVYVEAIGDVAFRVAP
                     IGALEARDLLTGIRGSAILRGIRGAPPVDFRALGDILRRIGQLGVDFPEIVEMDANPI
                     LAFPDGARAVDARVLLRQP"
     gene            4249..5835
                     /gene="aceB"
                     /locus_tag="VFU75_07995"
     CDS             4249..5835
                     /gene="aceB"
                     /locus_tag="VFU75_07995"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HEU5466817.1"
                     /translation="MLALPTIQVRPSRTADGARVLTPDALAFVARLAEKFESTRRALL
                     ERRTMRQADFDRGVLPDFLAETQGVRRASWKVAPPPKDLEDRRVEITGPVERKMMINA
                     LNSGARVFMADFEDALSPTWENVVQGQANLQDAIRRTLAFRSPEGKSYQLNDKIATLV
                     VRPRGWHLDERHATLGDAPVSASLFDFGLYFFHNAKELLARGSGPYFYLPKLESHLEA
                     RLWNDVFTFAESALGVPHGTIRCTVLIETILGAFEMEEILHELRDHGPALNAGRWDYM
                     FSIIKKFRTQRAMVLPDRVQVTMTVPFMRAYTELLVATCHARGAHAIGGMAAFIPSRK
                     DPEVNARAIAKVTEDKDREAGDGFDGTWVAHPDLVPVAQASFDKVLGARPNQKERLRD
                     DVRTTAAQLLSLAIPGSSVTEAGVRTNVTVALQYLASWLAGVGAAGINNLMEDAATAE
                     ISRAQLWQWIRNKTVLADGRTMTTDLYAAVRDEERKKLGALPHLDAAIRLLDGLVLAS
                     EFTEFLTIPAYRELDRAQGG"
     gene            5837..7141
                     /gene="aceA"
                     /locus_tag="VFU75_08000"
     CDS             5837..7141
                     /gene="aceA"
                     /locus_tag="VFU75_08000"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: protein motif:HMM:NF011645.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004451 - isocitrate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HEU5466818.1"
                     /translation="MSTETLRDIAALERRWANDSRWTGIKREYTAADVVSLRPSLRIE
                     HSLARAGAHRLWHQLEATPYVQTFGALTGAQAVQMVKAGLQAIYLSGWQVAADANLSG
                     QTYPDQSLYPSNSVPALVKRLNQALMRADQIERAESGHATLDWYAPILADAEAGFGGP
                     LHAFELMKQMIEAGAAGVHFEDQLASEKKCGHLGGKVLVPTQQFIKTLVAARLAADVL
                     DVPTLIVARTDALSATLLTSDVDPRDREFVTGERTPEGYFRVRDGLEPVIIRALAYAP
                     YADLLWFETSTPDLEEARLFAEAIHAKFPGKRMAYNCSPSFNWKQHLGDAEIAKFQES
                     LAQMGYKFQFITLAGWHLLGLATFDLARAYRDEGMTAYVRLQQEEFAREAEGYTATRH
                     QREVGAGYFDRVLAAVTGGEASTSALKGSTEAEQFRATGSGR"
     gene            complement(7122..9212)
                     /locus_tag="VFU75_08005"
     CDS             complement(7122..9212)
                     /locus_tag="VFU75_08005"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HEU5466819.1"
                     /translation="MRVTHVALLLMLVPAPFRGIRAQEPASDSLLTVNHYLDMEGVSD
                     PQLSPDGTQILYTRRWVNKLEDKFESAIWAMRADGSHNRLLVKGSSPRWSPDGSRILY
                     VAEGEPKGAQIFVRWMDAEGATSQVTRTEHAPGDPKWAPDGKSIAFVMFVPEADNWKI
                     PMPAAPEGAKWTPAPRMEDRLHYRQDRIGFTEPGFTHLFLVSADGGTPRQLTSGNWNV
                     GARFDGLTGGAGLAWTSDGKSILFDGLMDTTGDYAYQSSQIYRVEVASGAIKRLTTRP
                     GYWANPVISPDGRLVAYAGYDSSAKSHSTAALYVMSTDGSQSRKIDGTLDRDPGELTW
                     AGDNSGLFFTVQDTGTQNVHYAALAGGVKTITTGTHLLSLNSMAKDLTAVGTETDFKH
                     PGNVVRYSLKKFDGTTRLTSVNDDVLAGKHLAQEQEIWFRSSGNARVEAWIVTPPGFD
                     SHKKYPLILEIHGGPYAEYTVGWSYMFQNFAADGYVVLYVNPRGSTGYGTAFSDGIDH
                     NYPGPDYDDLMAGVDTVIGRGIVDTTKMFVSGCSGGGVLSSWVIGHTQRFAAAAVRCP
                     VIDWISMTGVTDVPLFTYSLFNDPWWDKPDPWLAHSSLMYVGHITTPTLLMTGELDRR
                     TPIPQSEEFYSALKMRHVPTVLLRFADEFHGTGSKPSNFMRTQLYMMSWFQKWSGGAK
                     GATRQDGQVSDRTP"
     gene            complement(9266..10231)
                     /locus_tag="VFU75_08010"
     CDS             complement(9266..10231)
                     /locus_tag="VFU75_08010"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HEU5466820.1"
                     /translation="MKFLSVLAILPAALVAQAQQTNYPQPKLPPPDGYRVYIIPDMEG
                     MGSVVNIHEVIAGIEGKGYANLTGPDYWGHSRQLMTQEVNAVIRGSRRSGARSFVVNE
                     GHGGNLFANIIPEDLDTGALLIRGFPKPLIMITGIDSSVGTVIFTGAHAGPGRPGVMA
                     HNYSFDHFAVDGHELNEVGINALIAGEFGVGVSMVTGDDGEIAQCREILGDKFVGVIT
                     KWALSRGAAVTYSPIKMRRILADSAAKAVRLARAHTFRPYTLPKPYQVEFTLRASYPD
                     SVVMGVDSLHWPGLEKISGRTYHLTTESAVQMGYLLDAIESVVLK"
     gene            10355..11227
                     /locus_tag="VFU75_08015"
     CDS             10355..11227
                     /locus_tag="VFU75_08015"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEU5466821.1"
                     /translation="MRWSQLLDKVRLWFDTTAAPVSTAQRVDWVRVIPFVLMHAACLG
                     VIWVGWSWTAVTVAVALYVLRMFWVTGFYHRYFSHRTFNTSRPAQFVFAVLGNTAVQR
                     GPLWWAAHHRRHHRYADTEQDVHSPRQHGFWWSHMGWITSDGNFRTDFSQVTDLARFP
                     ELRFLDRFDILVPLLLATILFLVGGWRLLIWGFFISTVVLFHCTCLINSLAHTLGRRR
                     FDTPDDSRNSLILALITLGEGWHNNHHRYPGAVRQGFRWWEVDFTYYGLRMLALVHLI
                     RDLHPVPRRLLQST"
     gene            11323..12432
                     /locus_tag="VFU75_08020"
     CDS             11323..12432
                     /locus_tag="VFU75_08020"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HEU5466822.1"
                     /translation="MFGNGSGPAAHLHVKDSQFFRAIAFGGALGAAEAYLNGWWESDD
                     LVALVRVLVRNRAALEGLERGLARLAKPFRALAHALRRNTREGSRRNIAAHYDLGNEF
                     FRLFLDDTLAYSCGIFETPEASLHDASVAKFDRICRVLELSPKDQLLEIGTGWGGFAM
                     HAAREYGCHVTTATISKQQELLANQRITDAGLSGRVTVVNCDYRDLDGTFDKLASIEM
                     IEAVGHQYLNGFFRACADRLKPDGLFALQAITIQDRFYRAALHEVDFIKAYVFPGSFI
                     PSRAVLAESAGLTDLTLVDLHDLTPHYAETLKHWRENFLSRREDVLGLGFDERFVRLW
                     EFYFAYCEGGFRERVLGDAQLLFAKPENRRDALSR"
     gene            12521..13624
                     /locus_tag="VFU75_08025"
     CDS             12521..13624
                     /locus_tag="VFU75_08025"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HEU5466823.1"
                     /translation="MLATQVKFQSRASTAFMDELKERVDGYFASRHLSPKANFSMVVK
                     SVILLGTYFGGYALLMTNWLSPWPMLGVALIMGVAMAGIGFAVSHDALHGAYSADPRI
                     NRVIGYTFDLLGANGYMWKITHNVIHHTYTNIHGVDEDIAVSPLIRMSPRSPWHPVHR
                     IQQYLAFVLYSFSTLFWVFVKDYKYFLATNLGPYANKKHPRGEIVSLVLTKAVYYAYT
                     IVIPLLVLHVTWWQFAIGFLAMHLTAGLILGVVFQLAHVVEGPQQPVCDCNGMIESCW
                     PVHQMLTTADFATHNRLLSWYVGGLNFQVEHHLFPKVCSVHYPALNPILVEVAAKHGV
                     PYYSNPTLWSAIRSHYETLKEFGRAPESVPATA"
     gene            complement(13636..17094)
                     /locus_tag="VFU75_08030"
     CDS             complement(13636..17094)
                     /locus_tag="VFU75_08030"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012907.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5466824.1"
                     /translation="MTPNSSTQMFLDPTGRRRRNVRLGAWVLGIITVAAGIFVATGLV
                     IPPLLPDLPLRQTASDSLGRVPRTVKPQFRQERISVAAERERVAAREKLFEHLKQNPA
                     PPARRLEQMGSRRSASSTAVKSRVPTDPIVAGFYVNWDDNSRVSLDTNKNNIDWIVAE
                     WGLVRKRARDSLPLVIQVKDSVFYYQQQARKPRPGIFLMLTNATGDGFDPGAVRTMIG
                     RPSFRRRSIKMIVDTVTALNLAGVTIDFEEVDAALHPMILSYLRQLRHALPTDKLLTQ
                     AVPGDDPSWPLEAYAAVDDKIFLMDYDEHDPSDDPGPVASQRWFEDNLEHALRRIPAR
                     KLIVGIGQYGYQWSDTSESATELTFQDAMQLARDNHLQPSMDHEALNPTFSWDDKDSV
                     TSIVWYLDAPTAWNEIKYALARGVAGVGIWRLGAEDPSLWNVLHRDGLAGNPAPMDTM
                     RIGYDVNFIGTGEILRMVAEPTLGQRTMTIDSVTKTVTGEVVTQTPSTWVIRRYGRRK
                     HAVAFTFDDGPDGKWTPMILDTLESRGVTATFFVIGENAAIHADLLRRELAEGMEIGN
                     HTYSHPNLALVGRGTTRLELSATERLIEAFLNRRTALWRPPYFGDAEPTTLDELVPVS
                     IGQELGYITVGLHIDPSDWSSPGTQTIIDSTLKQLNRGNIILLHDGGGDRSQTVAALG
                     PLIDSIRARGYAFTTVTALAGVSTTQAMAPLPPGSTFRHFVELTSYTVLGRLELTMHA
                     VFLIAMLLGMGRLAVILALAARQRYARRHQGPEPAHADYHPSVTVIIPAYNEDRVVCR
                     TVRTVLDQGYAGLEVLVVDDGSPDRTSDVVREAFGDDPRVKLFRKPNGGKASALNFGV
                     ERSTGDVIVALDADTLFPAGAIAALVKPLADPKVGAVAGNAKVGNRINIVTRWQAVEY
                     VTSQNIDRRAFSLLNCITVVPGAIGAWRKELILESGGFSSQTLAEDQDLTMTILERGW
                     RIAYADKATAYTEAPDTLRGLAKQRFRWSFGTLQCAWKHKYALLRAKYGMFGFVGLTN
                     IWIFQLLFPFISPVADLMFLYSLAKVYQNGVEHGPEYAWQTLEQVIFFYSVFLSVDFL
                     AAVVALLMEPDEDRSLAWLILIQRFAYRQVMYWVVVKSVFAAIQGTARGWGKQERKGT
                     VALNSG"
     gene            complement(17131..17895)
                     /locus_tag="VFU75_08035"
     CDS             complement(17131..17895)
                     /locus_tag="VFU75_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466825.1"
                     /translation="MKTITLLALVLAAAPLAAQDNYEIQVYGADLVDPHHTMLELHSN
                     YTFDGRSTVVDGMFPTRHALHETIEITHGFTPWFETGFYVFTSARAGQGWDWVGDHIR
                     PRFRVPESWHWPVGVSLSQEIGYQRRAFSPDTWTWEIRPIIDKRVGKLYWAVDPSLEK
                     SLRGENAGKGFEFSPNVSLTYDLTEQVTLGVEYYGALGPLSGFDPLNGQEQQIFPAVD
                     LNLSPDWEFNLGAGFGLTPGTDRFILKMITGYRLKF"
     gene            17944..19143
                     /locus_tag="VFU75_08040"
     CDS             17944..19143
                     /locus_tag="VFU75_08040"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5466826.1"
                     /translation="MAILISPTRGRNAAILAGFLGWTLDAFDYFLVVLTLTAIGQEFH
                     RSDAAIGFSLLLTLAFRPVGALVFGLLSDRFGRRLPLMINLVFYAVIEVLTGFSTSYG
                     MFLVLRALFGIGMGGEWGVGASLVMEKVSPKWRGFFSGLLQQGYALGYLLAALAYWYV
                     FPRWGWRPLFFIGGLPALLAIFVRAQVSESEVWLKTREPTWGGLGRTLKSQIPLFLYL
                     TVLMALMNMSSHGTQDLYPTFLKRAWQLTPRGVSWVTIVSMIGALIGGIVFGHWSERL
                     GRRKTITLTLLGAVLVVPLWAFAPSLALLVVGAFLMQFLVQGAWGVIPAHITELVPDQ
                     VRGALPGFAYQCGALISGAMPFLQPKVAETLGYPATMALSAGAAFLICALVTSLGKEK
                     RGVVYGA"
     gene            complement(19146..21578)
                     /locus_tag="VFU75_08045"
     CDS             complement(19146..21578)
                     /locus_tag="VFU75_08045"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEU5466827.1"
                     /translation="MRNRMMSMLSAALTIPMLLAAQRQTGVIGQYTAPRVWKPEARRF
                     DLLHQKIEMRFDVPHRELFATVTTRVAITLAPTDTIFLNAENLTIDGASDDHGRTLRF
                     TQDTAHVTVKLAHRAAVGDTVQFSLTYHTVPERGLYIVPRKNIVWSQGEATETRAWIP
                     TYDASNDKTTWEFLVTADTGLKVLSNGKLIGVTPTKDGKANIWHWSQEKPASTYLYSV
                     VVGPFTVLKDQWRGIPVEYWTYADTVNAAWRAFGETPSMIELYSQILGVNFAWDKYDQ
                     SIIPDFTYGGMENVSATTQTDLALHSVSQEPENSARSLNAHELAHQWFGDLTTTADWA
                     DIWLNEGITTYMESVQDEKTRGWDDAELNWYGQQQQAMGADQNEVRSLVWGKYQGTDP
                     IALFFSGHVYPKGAQLAHQLRRLLGDSVFWAGMHRFLVDNAYKPVTTADYAIAMEKTC
                     NCDLDWFFDQWAYGIGYPKVEFHRHWAANLKTLHVVVSQTQPIDSLHPVFQFPATIRV
                     ITRDSVIRQQIFVHHQTDTFAIALPAEPLSFRFDEGGWLLGTVKGDLSVAELADEAKH
                     DLDIRGRRWALGQLEGNMDPAAVETRRFLVLNDHTEWLREVATRMMAHDANPESKGVL
                     TSALRDPEPQVRMQALQSLDSLDAAQALTVANAMYTTDPNDDVRAVALSVIASQKGKD
                     ALPTLLAAVGPDQIANMRFTAMGFLSRIRDGKAEDALERNTATTEDRNIRQVALQALA
                     ATGDSARATAVALRLIPDYDPLFAVTAVRVVGAVGGEAGKAKLRDAIKKETRVFVRAA
                     MERALTATPMKM"
BASE COUNT         3628 a         7273 c         6935 g         3936 t
ORIGIN      
        1 aggtcctcac gagcggttgg tgacgaatgt gacggcgcga ctgacgacgg acggatcccg
       61 caagacgccg cgatgtccga gcccctgcgt caacatcaac tcggcaccgg gccacgtggc
      121 cgcgatctcc ctgccttcat ctaccggaat cgtctcatcc tgctgatcat ggaccaccag
      181 ggccgggctt cgggccccgg caaacaccgg cagcacgttc aattgatccc agtcaaaccg
      241 gatgcggcgt tcactgcggg accggagcgc agcacccgtc cgttcagaca gcctcagcgc
      301 ccgcacgaag tcgtcgacga atcgcaatgg gaacgccgcg ggggcaatca gcgccacccg
      361 ccggggctcg aagcccttgg acatcgccag tgcggtcacc acgcccccga acgaatgggc
      421 gatgatcgcg tcgggcgcac cgacctcggc catcacggcg tcgagggcgc gcgccatttc
      481 cggcgccgac gacatccccc gtccggtcag gccatgcccg ggaccgtcgt acgtgacggc
      541 actgaagcct cgggcgagaa tcggatcgac gaacgaagag aaccgcccac cggctcctcc
      601 ccatccgtgc accaggaaca ccatgggccc cgttccccaa cgccaggccc ggatctcgtg
      661 tccctcggag cgaaccgtaa atgcccgggc ccgtccgagt gcatcgcggt cgcccgtaga
      721 aaggggccga tgtggcgggg tgaagaacag ccgctccgcc caccagcccg ccagctccgg
      781 ggacaccgct tgcgcgccgc gcaagccgag cgaaatcgcc gcgagtgaca ggttcgccat
      841 tggaatgacg gaatgtagct tactcgccgt ggataccgac gtcctcgtca tcggagcggg
      901 ccccggcggg ctcgccgcat cgcagcagct caagcggcac ggcgtccccc ataaggtgct
      961 cgagcgcggc gacaccgtgg gccatacctg ggccaatctc tacgattcgc tcgtcctcca
     1021 caccggaaag cggatgtcga gtttaccagg cttggcgttc ccggccgact acccgacgtt
     1081 tgtaagccgc gccgacttcc tgaagtacct cctccaatac cgggaaacgt tcgaccttcc
     1141 ggtggaaacc gggaagccgg tctcgcggct ggagcggacg gccgccggct ggacggctca
     1201 cgtgaacggg gatcggatca gctgccggag cctcgtcatg gcgaccggga tcgtcgcgaa
     1261 tcccgtcgcg ccgcgcttcc ccggccagga tcgattcaag ggccacgtaa tgcacagcgt
     1321 cgagtatcgc aggcccgacc cgttccggga ccagcgcgtg ctcgtggttg gggtgggaaa
     1381 ctcgggtggt gagatcggcg ccgagctggc ccaccagggc atcccggtca ccctggccgt
     1441 gcgctcgggg gcgaacgtcg tccctcggga gctcctcggc attccaattc agtatgtcgc
     1501 ctactacgtg cggaaacttc cgcgccccgt tcagcggatc atcgtctccc tcgtccggca
     1561 gatcacgatc ttgcgaaggg gtccgccggt actgccgccc tcgcccgtct tcgcgctcga
     1621 cgcggtcccg ctgatcggct ttcacctcgt ggatgcgatc aaggcgggaa ccgcgcaagt
     1681 caggcccggc ctacaggaat tcacggccaa tggcgtgcgg tttaccgatg gccgtgaaga
     1741 gtccttcgac accgtgatcc tggcgaccgg gttccacgcc gccctggccc ccctcggcgc
     1801 tttggtgact accgacgcca aggggtttgc ccgccgggcc gaccgggtca ccagcgcgga
     1861 tcaggaaggc ttgttcttcg tgggctcgaa ttacgattcg ggtggaggtc ttctcaacat
     1921 cggcatcgac gcccgactgg tcgggaggat cctggcgcaa tcctaaatcc gtcatgaaga
     1981 aaccttgcgg acgactccac gcatgtcctg tcttcataat tttcctatgt gacgattctc
     2041 gattccgtcc tcaagcccgc cagcatcgcc gtgatcggcg cctcacgcac gccgaacacc
     2101 atcggacatc agatcatggc gaatctgctg cgctacgggt acaccgggcc cgtgtatccg
     2161 gtgaacccga aggcctcctc ggtgcattcc gtccgggccg cgccgtcgat ccgcgcgttg
     2221 tccgagcccg tggacctcgg tgtgatcgtg gttcccaagg agcaggtgct cgaagtcgcg
     2281 gccgaatgtg ccgaggccaa ggtgcggggg ctggtggtca tctccgccgg ctttcgcgag
     2341 atcggtgggg cgggcgccga gcgcgagcgg cggctcctcg atatcgtgcg ctcgaatcgg
     2401 atgcgcatgg tcggtcccaa ctgcatgggg gtgctcaacg ccgatccggc gttctccatg
     2461 aactgcacct tcgcgcccac catgcctccc ttcgggcacg tcgcgttcgt gtcccaatcg
     2521 ggggccctgg gcgtgagtgt gctggactac accaaggagt acggcatcgg gatcgcccag
     2581 ttcgtgtcca tgggaaacaa gccggatgtc tcggggaacg acctcctgct ccaatgggaa
     2641 cacgatgagg cggttcgggc catcctgatg tacgtcgaga acttcgggaa tccgcaacgc
     2701 ttcctcgaga tcgcgtcgcg tatcacccgg cggaagccga tcatcgtggt gaaatcgggc
     2761 cgctcgcggg tcggcgcccg cgcggcggcg tcccacacgg gcgccctggc ggcgagtgac
     2821 gccgcggtcg acgcactgct gacgcaggcc ggcgtcctcc gcgccgattc catcgaagaa
     2881 ctcttcgaca tggccatggc cttcggcgct caaccgctcc cgcgctcgcg ccgcacggcc
     2941 gtcctcacga attccggtgg ccccggcatc ctcgcggcgg acgccctcga gcgtcatggc
     3001 ctcgaggtca ctgagctgga tgcggctacc gtctcgaggc ttcgccccct cttccccgag
     3061 gaggcctcca tccgaaatcc cctggacatg atcgcgtccg ccacgcccga aggatatcgg
     3121 accgcgctcg acgcgatgct cgacgacccc aaggtggatt cggccgtggc gatcttcgtt
     3181 ccaccgctcg gcctttccca gggcgcggtg gccgaggcca tcgtcgccgc ggcggcgaag
     3241 catccgacca aaccggtcct cggcgtgttg atgggccgcg aaggcctgcc gcaaggaaag
     3301 gcggagctcc atcgcgccgg aattccggcg tacatcttcc cggagtccgc agcccgcgcg
     3361 ctcgcggcgt tgcggcggca ccgggagtgg caggcccgac cggtcgctac cgaggcgccc
     3421 ctcaccgtcg atcgcgaggc cgcccggaac atcttgtgtg cggcgcgggc ggagggacgt
     3481 gcccggctcg atgagatcga gtcgatccac ctgctggcgg cctacggcat tcccaccacc
     3541 tgcacgcgat tcgcgacgga ccccaatgac gccggccagg tggctggaga gatcggattc
     3601 ccgatcgtgc tcaaggtcgt ctccccggat atcgtgcata agagcgacgt cggcggtgtg
     3661 cgggtcgggc tggcgacccc catggaagtg cagatcgccg cggcccagat gctggaggat
     3721 gtgcgacacc gggcaccgga cgcgacggtg cgggggttcg tcattcaacg catgatcagc
     3781 gggggccggg aaaccatcgc cggcctgacg cgggaccccg ccttcggccc actggtcatg
     3841 ttcggcctgg gaggcgtgta cgtcgaagcc atcggagacg tcgccttccg cgtagcgccc
     3901 atcggcgccc tcgaggcccg cgatctcctc accggcatcc gcggcagcgc catcctgcgc
     3961 ggcatccggg gagccccgcc cgttgacttc cgcgccctcg gcgacatcct gcgacggatc
     4021 ggccagctcg gagtagactt ccccgaaatc gtggagatgg acgcgaaccc cattctcgcc
     4081 ttccccgacg gggcccgggc cgtggacgcc cgcgtcctcc ttcgccaacc gtagcctccc
     4141 tgccgtacct gtcgcacccc tgccacacgc gggagtttgc gcatcgctac catgcgtgtt
     4201 tctacttggc ccggaacttg attcgaatcc tgactcatgg agagaccgat gctcgccttg
     4261 ccgacaatcc aggtccgtcc gtcccgaacc gccgacggcg cgcgggtgct cactcccgat
     4321 gccctcgcct ttgttgcgcg tctggccgaa aagttcgaat ccacccgccg cgcattgctt
     4381 gaacgccgga cgatgcggca agcggatttt gaccgcggtg tgctccccga ttttctcgcc
     4441 gagacccagg gagtccgccg cgcctcgtgg aaagtcgcgc ctcctcccaa ggacctcgaa
     4501 gaccgccggg tcgagattac gggccccgtc gagcggaaaa tgatgatcaa cgcgctcaac
     4561 tcgggtgcgc gcgtcttcat ggcggacttc gaggacgcgc tttcgccgac gtgggagaac
     4621 gtggtccaag gacaggccaa tctgcaggat gcgatcaggc ggaccctcgc gttcaggagt
     4681 ccggaaggaa agtcgtacca gctcaacgac aagatcgcga cactcgtggt ccgaccccgc
     4741 ggctggcacc tggatgagcg ccacgcgacg ctaggcgatg ccccggtttc ggcttcgctg
     4801 ttcgacttcg ggctctactt cttccacaac gcgaaggagc tcctggcgcg cgggtcgggc
     4861 ccgtacttct atctgcccaa gctcgagtca cacctcgagg cgcgtctctg gaatgacgtc
     4921 ttcacgttcg cggagagcgc cctcggcgtc cctcacggca ccatccgttg caccgtcttg
     4981 atcgagacga tactcggcgc gttcgagatg gaggagatcc tgcacgagct tcgggaccat
     5041 ggccccgcgc tcaacgccgg ccgctgggac tacatgttct cgatcatcaa gaagttcagg
     5101 acccagcgcg ccatggttct gcccgaccgc gtccaggtca ccatgacggt ccccttcatg
     5161 cgggcgtaca cggagctcct ggtcgccacc tgccacgccc gcggcgcgca cgccatcggc
     5221 ggaatggccg ccttcattcc gagccgcaag gaccctgagg tgaacgcccg ggccatcgcg
     5281 aaggtgacgg aggacaagga ccgcgaggcc ggggacggat tcgacggtac ctgggtagcg
     5341 cacccggacc tcgtcccggt cgcgcaggcg tcgttcgaca aggtcctggg cgcacgcccg
     5401 aatcagaaag agcgcctccg ggacgacgtc cggaccacgg ccgctcagct cttgtctctc
     5461 gccatccccg gctcttcggt gaccgaggcg ggggtccgca ccaacgtgac cgtggccctg
     5521 cagtacctgg cgagctggct cgcgggggtc ggggcggcgg ggatcaacaa cctgatggaa
     5581 gacgccgcga ccgcggaaat ttcccgggcg cagctctggc aatggatccg caacaagacc
     5641 gtcctcgccg acggtcggac catgaccacg gacctgtacg ccgccgtgcg ggacgaggag
     5701 cgcaagaagc tgggcgccct tccccacctc gatgcggcca tccggctcct cgacggcctg
     5761 gtccttgcat cggaattcac cgaattcctc actatcccgg cctaccggga gctcgatcgc
     5821 gcacaggggg gatgagatga gcaccgagac tctgcgggat atcgccgcgc tggagcgacg
     5881 ctgggccaac gattcccgct ggacgggaat caagcgggag tacacggcgg ccgacgtcgt
     5941 gtccctccgc ccctcactcc gcatcgagca ctcgctcgcc cgcgccgggg cccaccgcct
     6001 ctggcaccag ctggaggcca ccccctacgt ccagaccttc ggcgcgctca ccggcgcgca
     6061 ggcagtccag atggtgaagg cgggactcca ggcgatctat ctgagtggat ggcaggtcgc
     6121 ggccgacgcc aacctctcgg gtcagaccta tcccgaccag agtctttatc cctcgaacag
     6181 cgtccccgcg ctggtcaagc ggctgaatca ggcgctgatg cgcgccgacc agatcgagcg
     6241 cgcggagtcc gggcacgcga cccttgattg gtacgccccc atcctggccg acgccgaagc
     6301 gggcttcggg gggccgctgc acgccttcga actgatgaag cagatgatcg aggcgggtgc
     6361 cgccggcgtg cacttcgaag accagctggc gtcggagaag aagtgcgggc atctgggagg
     6421 caaagtcctg gtgcccaccc agcagttcat caagacgctg gtcgccgcgc gcctcgccgc
     6481 cgacgtgctg gacgtgccga ccctgatcgt ggcccgcacg gatgccctct ccgcgaccct
     6541 cctgacctcg gacgtcgacc cgcgcgaccg ggagttcgtc accggcgaac ggacgcccga
     6601 ggggtacttc cgggtccggg acggcctcga gcccgtcatc attcgggcac tggcctacgc
     6661 gccctacgcc gacctgctct ggttcgagac gtcgacgccc gatctcgagg aagcgcggct
     6721 gttcgccgag gcgattcacg ccaagttccc cggcaagcgc atggcctaca actgctcccc
     6781 ttcattcaat tggaagcagc atctgggcga tgccgagatc gcgaagtttc aggaatcgct
     6841 ggcgcagatg ggatacaagt tccagttcat cacactcgcc ggctggcatc tcctgggact
     6901 tgccacgttc gacctcgcca gggcgtaccg cgacgagggg atgaccgcgt acgtgcggct
     6961 gcagcaggag gagttcgcgc gggaggcgga ggggtacacc gccacccgcc accagcgcga
     7021 ggtcggagcg ggttacttcg accgcgtact ggcggcggtg acgggtggtg aggcctcgac
     7081 gtcggccctg aaggggtcga ccgaggccga gcagttccgg gctacggggt ccggtcgctg
     7141 acctggccgt cctggcgggt ggcgcccttg gcgcccccgc tccacttctg gaaccagctc
     7201 atcatgtaga gctgcgtacg catgaagttc gacggcttgg agccggtgcc gtggaactca
     7261 tccgcgaagc gcaacaggac cgtcggcacg tgccgcatct tcagggcgga gtagaactcc
     7321 tcgctctggg ggatgggcgt gcggcggtcg agctcgccgg tcatcaggag cgtgggcgtc
     7381 gtgatatgac cgacgtacat gagcgacgaa tgcgcgagcc aggggtccgg cttgtcccac
     7441 cagggatcgt tgaacaggct gtaggtgaag agcgggacgt cggtgacgcc cgtcatgctg
     7501 atccagtcga tcaccgggca ccgcacggcc gccgccgcga agcgttgggt gtgaccgatc
     7561 acccagctcg acagcactcc accccctgag catcccgaca cgaacatctt cgtcgtgtcc
     7621 acgatgcccc ggccgatcac ggtgtccacg cccgccatca ggtcgtcgta gtcggggccc
     7681 ggatagttgt gatcgatgcc gtccgagaac gccgtgccgt agccggtaga gccgcgggga
     7741 ttcacgtaca gcacgacgta tccatcggcc gcgaaattct ggaacatgta gctccagccc
     7801 accgtgtatt cggcgtaggg cccgccatga atctcgagaa tgagcgggta cttcttgtgc
     7861 gagtcgaatc ccgggggcgt cacgatccac gcctcgacgc gcgcgtttcc gctcgaccgg
     7921 aaccagatct cctgttcctg cgcgagatgc ttcccggcca gcacatcgtc attgacggac
     7981 gtcaggcgcg tcgtcccgtc gaacttcttg aggctgtacc ggaccacgtt gcccggatgc
     8041 ttgaaatcgg tttcggtacc gaccgccgtg aggtccttcg ccatcgagtt gagcgacaag
     8101 aggtgcgtcc cggtggtgat cgtcttcacg ccgccggcca gcgcggcgta gtggacgttc
     8161 tgcgtgccgg tgtcctgcac cgtgaaaaag agtccgctgt tgtcgcctgc ccaggtgagc
     8221 tccccggggt cccggtcgag agtgccgtcg atcttccggg attgggatcc gtcggtgctc
     8281 atgacgtaaa gtgcggcggt ggagtgcgac ttcgccgagg agtcgtaccc ggcatacgcc
     8341 accaggcgcc cgtcgggaga aatcacggga ttcgcccagt accccgggcg cgtcgtcagc
     8401 cgcttgatcg cgccggacgc gacctcaacc cggtagatct gtgacgactg gtacgcgtag
     8461 tcaccggtcg tatccatcaa gccgtcgaag aggatcgact tgccgtcgga cgtccacgcc
     8521 agtccggcac cgcccgtgag cccatcgaat cgcgcgccca cgttccaatt cccgctcgtc
     8581 agctgacgcg gggtgccgcc gtccgccgac accaggaaga ggtgcgtgaa gcccggctcg
     8641 gtgaaaccga ttcgatcctg ccggtagtga agacggtcct ccattcgcgg cgccggcgtc
     8701 cacttcgctc cttccggagc ggccggcatg ggaatcttcc agttgtccgc ctccggcacg
     8761 aacatcacga aggcgatcga cttcccgtcg ggagcccact tggggtcccc cggcgcgtgc
     8821 tcggtccggg tcacctgact ggtcgcgccc tcggcgtcca tccagcggac gaaaatctgc
     8881 gcgcccttgg gttcaccctc ggcgacgtag agaatgcgcg agccatcggg cgaccagcgg
     8941 ggcgaggagc ccttcaccag cagccggttg tgcgacccgt cggcgcgcat ggcccagatg
     9001 gcggactcga acttgtcttc cagcttgttc acccagcggc gggtgtagag gatttgcgta
     9061 ccgtccggtg aaagctgggg atcggacaca ccttccatgt cgagatagtg attgacggtc
     9121 aggagggagt ccgaggcggg ttcttgagcc cggattcccc ggaacggcgc ggggactagc
     9181 atcaggagca gagcgacgtg ggtgacgcgc atgacgacct ccaatgaccg ctgatggctg
     9241 acggctgacg gctgacggct gatcgctact tcaacacgac actctcgatg gcgtccagca
     9301 gatagcccat ctgcaccgcg ctctccgtgg tgagatggta agtacggccg gagatcttct
     9361 cgagccccgg ccagtggagt gaatccaccc ccatcacgac gctgtcggga taactcgccc
     9421 gcagcgtgaa ttcgacctga tagggcttgg gcagcgtgta cggccggaac gtgtgcgccc
     9481 gcgccaggcg gacggccttc gcggcggagt cggcgaggat ccgccgcatc ttgatgggac
     9541 tgtaggtgac tgcggcgccg cgcgagagcg cccacttggt gatcacgccg acgaacttgt
     9601 cgccgaggat ctcccggcac tgcgcgatct ctccgtcatc cccggtcacc atggacacgc
     9661 ccaccccgaa ctctccggca atgagggcgt tgatgcctac ctcgttcagc tcgtgcccat
     9721 ccaccgcgaa gtggtcgaac gagtagttgt gcgccatcac gcccggacgc ccgggtcccg
     9781 cgtgtgcgcc cgtgaaaatc acggtcccca cgctcgagtc gatccccgtg atcatgatca
     9841 aaggtttcgg gaatcccctg atcagcaatg ccccggtgtc gaggtcttca ggaatgatgt
     9901 tggcgaacag attaccgcca tgcccctcgt tcaccacgaa cgaccgggcc cccgaccggc
     9961 gcgagccccg gatgaccgcg ttgacttcct gcgtcatgag ctgccgggaa tggccccagt
    10021 agtcgggacc cgtgagattc gcgtaccctt tcccctcgat cccggcgatc acctcgtgga
    10081 tgttgaccac gctccccatc ccctccatat ccgggatgat gtaaacgcga tagccgtcgg
    10141 gaggcggcag cttcggctgc ggatagttcg tttgttgcgc ctgggctacg agtgcggcag
    10201 gaagaatcgc gagaaccgag aggaacttca cgaggcgctc cgaggttggc gctattgggc
    10261 tgacgtctga cggctgtttg tggctaagag agcacctccc ccgggcaggg gcaagagctt
    10321 cgtcatcacc agaaacgggg gttagattag cggcatgcga tggtcgcaac tgctcgacaa
    10381 agtccggctc tggttcgaca cgacggccgc accggtttcg acggcgcaac gggtggactg
    10441 ggtgcgggtg atccccttcg tcctgatgca cgcggcctgt ctcggcgtga tctgggtcgg
    10501 gtggagctgg acggcggtga cggtcgcggt cgccctctac gtactccgaa tgttctgggt
    10561 caccggattc taccaccgct acttctcgca ccgcaccttc aacacttcgc ggccagccca
    10621 attcgtcttc gcggtcctgg gaaacacggc ggtacagcgc gggcccctct ggtgggccgc
    10681 gcaccatcgc cgccatcacc ggtacgccga caccgagcag gatgtgcact ccccgcgcca
    10741 gcacgggttc tggtggagcc acatgggatg gatcacatcc gacggaaact tccgcaccga
    10801 cttttcgcag gtgacggacc tcgcgcgctt tcctgagctc cgtttcctgg accggttcga
    10861 catcctggtc cccctgctcc tggccacgat cctgttcctc gtgggtggct ggcggctcct
    10921 gatctggggc ttcttcatca gtaccgtcgt tctgttccat tgcacctgcc tcatcaattc
    10981 actggcccac accctcggcc gccggcggtt cgacacgccc gacgacagcc gcaacagtct
    11041 catcctggcg ctgatcacgt tgggtgaagg ctggcacaac aaccaccacc ggtatcccgg
    11101 cgcggtgcgc cagggcttcc gctggtggga ggtcgacttc acgtattacg gcctccggat
    11161 gctcgccttg gtccacctca tccgcgatct ccaccccgtt ccgcgacggt tgctgcaatc
    11221 gacctgatac gtcgaacgct gtcccgccgg ctcgagcgca tcacgtacgg acggatcacg
    11281 ctcgacggcc aggacggcca ggacggccac gcgccggcgc aggtgttcgg gaacggctcc
    11341 ggtcccgccg cccatctcca cgtcaaggat tcgcaattct tccgcgccat cgctttcggc
    11401 ggggcgctgg gcgccgccga ggcttatctg aacgggtggt gggaatcgga cgatctggtg
    11461 gcgctggtgc gggtactggt gcggaaccgc gcggcgctcg agggactgga gcgcggcctc
    11521 gcgcgcctgg ccaaaccttt ccgggccctc gcccacgcct tgcgacggaa cacccgggag
    11581 ggcagccggc gcaacatcgc ggcgcactac gacctgggga acgagttctt ccggctcttt
    11641 ctcgacgaca ccctcgccta ctcctgcggg atcttcgaga cgccggaagc gagccttcac
    11701 gatgcgtcgg tggcgaagtt cgaccgtatc tgccgcgtgc tcgagctctc gccgaaggat
    11761 cagctgctgg aaatcgggac cggctggggc ggattcgcca tgcacgcggc gcgcgagtac
    11821 gggtgccacg tcaccaccgc caccatctcc aagcagcagg agcttctcgc gaaccagcgc
    11881 atcacggatg ccggcttgtc ggggcgggtc acggtggtga attgcgacta ccgggacctg
    11941 gacggaacgt tcgacaaact cgcgtccatc gagatgatcg aggccgtggg tcatcagtac
    12001 ttgaacgggt tcttccgggc ctgcgccgat cgcctcaagc ccgacggcct gttcgcactg
    12061 caggcaatca cgattcagga ccggttctac cgcgcggccc ttcatgaagt cgacttcatc
    12121 aaggcgtacg tcttccccgg ctccttcatc ccctcccgcg ccgtcctggc cgagtcggcc
    12181 ggcctcacgg atctcaccct cgtggatctt cacgatctga caccgcacta cgccgagacc
    12241 ctgaagcact ggcgcgagaa ctttctctcg cgacgggagg acgttctggg gctcggattc
    12301 gacgagcggt tcgtacggtt gtgggagttc tacttcgcgt attgcgaggg aggattccgc
    12361 gagcgtgtcc tgggcgacgc ccaattgctc ttcgcgaaac ccgagaatcg gcgggacgcg
    12421 ttgtcacgat agtgatacgc ctcacaacct tacagaacgg cctctttcgc cgactcgtac
    12481 caccccccat cttgggtcag gcttcttcga ggtaaaaccc gtgctggcca cccaagtgaa
    12541 gttccaaagc cgtgcttcca cggcgttcat ggacgagctc aaggagcgcg tggacgggta
    12601 tttcgcgtcc cggcacctct cgcccaaggc gaacttctcg atggtggtca agtcggtgat
    12661 cctgctgggg acctacttcg gcggctacgc cctgctcatg accaattggc tgagcccgtg
    12721 gcccatgctg ggtgtggcgt tgatcatggg tgtggcgatg gcggggatcg ggtttgcggt
    12781 ttcccacgac gcgctccacg gagcgtactc ggcggacccc cgcatcaacc gcgtgatcgg
    12841 ctatacgttc gatctgctgg gcgccaacgg gtacatgtgg aagatcactc acaacgtgat
    12901 ccaccacacc tacacgaaca ttcacggtgt ggatgaggat atcgcggtgt ccccgctcat
    12961 ccgcatgtct ccccgctccc catggcaccc cgttcaccgc atccagcaat acctggcgtt
    13021 cgtcctctac agcttttcga cgttgttctg ggtgttcgtc aaggactaca agtatttcct
    13081 ggcgaccaat ctcggtccct acgcgaacaa gaagcacccg cgcggcgaga tcgtgtccct
    13141 ggtgctcacc aaggccgtct actacgccta caccatcgtc atcccgctgc tggtgctgca
    13201 cgtcacgtgg tggcaattcg cgatcgggtt cctggccatg cacctcacgg cgggcctgat
    13261 cctcggcgtg gtgttccagc tggcgcacgt ggtggaggga ccgcagcaac cggtctgcga
    13321 ctgcaacggc atgatcgaga gttgctggcc ggtacaccag atgctgacca cggccgactt
    13381 cgcgacccac aatcgcctgc tctcgtggta tgtcggcggg ttgaacttcc aggtggagca
    13441 ccacctgttt ccgaaggtgt gctcggtgca ctatcccgcg ctgaatccga ttctcgtgga
    13501 agtcgcggcg aagcacggcg taccctacta ctccaacccg acgctgtggt cggccatccg
    13561 ctcgcactac gagaccttga aggagttcgg gcgggctccg gagtcggtgc ccgccaccgc
    13621 ctgacggctc cggcgtcagc cggagttgag cgccaccgtt ccctttcgtt cctgcttgcc
    13681 ccacccgcgg gcggtcccct ggatcgccgc gaacacgctc ttcaccacga cccagtacat
    13741 cacctgccgg tatgcaaacc gctggatcag gatcagccat gccagtgacc ggtcttcatc
    13801 cggctccatc agcagcgcga cgaccgccgc caggaagtcc accgacagga agaccgagta
    13861 gaagaaaatc acctgctcga gcgtctgcca cgcgtactcg ggcccgtgct cgacgccgtt
    13921 ctggtacact ttcgcgagcg agtacaggaa catcaggtcg gcgaccggcg agatgaacgg
    13981 gaagagcagc tggaagatcc agatgttcgt gagccccacg aacccgaaca tgccgtactt
    14041 ggcgcgcagc agcgcatact tgtgcttcca cgcgcactgc aacgtcccga aactccagcg
    14101 gaaccgctgc ttcgcgagcc cgcgcagggt gtccggcgcc tcggtgtacg ccgtcgcctt
    14161 gtcggcgtac gcgatccgcc atccccgttc gaggatcgtc atcgtcagat cctgatcctc
    14221 ggccagcgtc tggctcgaga acccgccgct ctcgagaatc aattccttcc gccacgcacc
    14281 gatcgccccc gggaccaccg tgatgcagtt gaggagggag aatgcgcggc ggtcgatgtt
    14341 ctggctggtg acgtattcca ccgcctgcca gcgggtcacg atgttgatgc ggtttcccac
    14401 cttcgcattg ccggccaccg caccgacctt cggatccgcc agtggcttca ccaatgccgc
    14461 gatcgcgcca gcggggaaga gcgtgtccgc gtcgagcgcc acgatgacgt cccccgtgga
    14521 gcgttcgaca ccgaaattca acgcgctcgc cttcccgccg ttcggcttgc ggaagagctt
    14581 cacccgggga tcgtccccga acgcctcgcg cacgacgtcc gaggtccggt cgggcgaccc
    14641 gtcatccacc accaggacct cgagtcctgc ataaccctgg tcgagtacgg tccgcaccgt
    14701 ccggcacacc acgcgatcct cgttgtaggc cgggatgatc acggtgaccg acggatggta
    14761 gtcggcgtga gcgggttcgg gtccctggtg ccgccgcgca tagcgctggc gggccgccag
    14821 ggcgaggatc accgccagcc gtcccatccc gaggagcatc gcgatcagga agaccgcgtg
    14881 catggtgagc tcgagccggc cgagcaccgt gtaggaggtc agctcgacga agtgccggaa
    14941 cgtggagccg ggcggcagcg gcgccatcgc ctgcgtggtc gatacgccgg cgagcgcggt
    15001 gacggtggtg aacgcatagc cgcgggcccg gatcgagtcg atcaggggac cgagcgcggc
    15061 tacggtctgg ctgcggtccc cgcccccgtc gtgcagcagg atgatgttgc cgcggttgag
    15121 ctgcttcagg gtgctgtcga tgatcgtctg ggtcccgggg gaggaccaat cggacgggtc
    15181 gatgtgaagg ccgaccgtga tgtagccaag ctcctggccg atcgagaccg gaacgagctc
    15241 gtcgagggtg gtcggttccg cgtctccgaa gtagggtggc cgccagagcg ccgtccggcg
    15301 attcagaaac gcctcgatca gccgttcggt ggccgagagc tccagacgcg tcgtgccacg
    15361 gccgaccagg gccaggttcg ggtggctgta ggtgtggttc ccgatttcca tgccctcagc
    15421 cagctcccgc cgaaggaggt ccgcgtggat cgcggcgttc tccccgatca cgaagaacgt
    15481 cgcggtgacc ccacgggact cgagggtgtc gaggatcatc ggcgtccatt tgccgtcggg
    15541 accgtcgtcg aaggtgaacg cgacggcgtg cttccggcgc ccatagcggc ggatcaccca
    15601 ggttgaaggc gtctgggtga cgacttcgcc ggtgaccgtc ttggtgacgc tgtcgatggt
    15661 catggtgcgc tggccgagtg tcggctcggc gaccatccgc aggatttcgc ccgtcccgat
    15721 gaagttcacg tcgtagccga tgcgcatggt gtccatcggc gccggattcc cggccagccc
    15781 atcgcggtgc agcacgttcc agagcgacgg atcttcggcg cccagccgcc agatgccgac
    15841 cccggccacc ccccgcgcga gagcatactt gatctcgttc cacgccgtcg gcgcatccag
    15901 ataccacacg atgctcgtga ccgagtcctt gtcgtcccag ctgaaggtcg gattcagggc
    15961 ctcatggtcc atcgacggct ggaggtggtt gtcccgcgcc agctgcatgg cgtcctggaa
    16021 cgtgagctcg gtggcgctct cgctcgtatc ggaccactga tacccgtact gcccgatgcc
    16081 gacgatgagt tttcgggcgg ggatgcgccg gagggcatgc tccaggttgt cctcgaacca
    16141 gcgctggctc gcgacgggtc ccgggtcgtc cgacggatcg tgctcgtcgt agtccatcag
    16201 gaagatcttg tcgtcgaccg cggcatacgc ctcgaggggc caggatggat cgtcgcccgg
    16261 caccgcctgc gtcagcagct tgtctgtcgg gagcgcgtgc ctcagttgac gcaggtacga
    16321 caggatcatc gggtgcagcg cggcgtcgac ttcctcgaaa tcgatcgtca cgcctgcaag
    16381 attcagcgcc gtgacggtat cgacgatcat cttgatggag cgccgccgga atgacgggcg
    16441 gccgatcatc gtgcgcacgg ccccgggatc gaagccgtct cccgtggcgt tggtcagcat
    16501 caggaagatc cccggccggg gcttcctcgc ctgctgctga tagtagaaca cgctgtcctt
    16561 cacctgaatc acgagcggca gagaatcccg ggcgcgtttc cgcaccagtc cccattccgc
    16621 gacgatccag tcgatgttgt tcttgttggt gtccaacgag acgcggctgt tgtcgtccca
    16681 attgacgtag aacccggcga cgatgggatc ggtgggcacc cgtgacttga ccgcggtgct
    16741 gctggccgag cggcggctgc ccatctgctc caggcgccgg gccggtggcg ccggattctg
    16801 cttcaggtgc tcgaaaagct tctcccgcgc cgccacgcgc tcccgttccg cggccaccga
    16861 gatgcgctcc tgacggaatt gtggcttcac cgtccgcggc acccgcccca gcgaatcgga
    16921 agcggtctgg cggaggggga ggtcggggag cagcggcggg atgacgaggc cggtggcgac
    16981 gaaaatcccg gccgccacgg tgatgattcc cagcacccag gcccccaggc ggacgttgcg
    17041 tcgccgccgc ccggtggggt cgagaaacat ctgggtcgag gagttgggtg tcacgacgtg
    17101 aaaactaatg ctgagacagg tgatgcgtac tcagaacttg aggcgatacc ccgtaatcat
    17161 cttcagaatg aaccggtccg taccgggggt gagcccgaac ccggcgccga ggttgaattc
    17221 ccagtcgggc gagaggttca ggtccacggc ggggaagatc tgctgctcct gcccgttcaa
    17281 cgggtcgaaa cccgagagag gccccagagc gccatagtac tcgacgccca gcgtgacctg
    17341 ctcggtgagg tcgtacgtca gggaaacatt cggagagaac tcgaagcctt tgcccgcgtt
    17401 ctccccacgc agggacttct cgagactcgg atcgacggcc caatagagct tgccgacgcg
    17461 cttatcgatg atgggcctga tttcccatgt ccacgtgtcg ggagagaagg cccgtcgctg
    17521 gtagccgatc tcctgtgaca gactcacccc caccggccag tgccaactct ccggaacccg
    17581 gaaccgtggc cggatgtggt ctcccaccca gtcccacccc tgcccggctc gagcactcgt
    17641 gaaaacgtag aagccggttt cgaaccaagg cgtgaaacca tgggtgatct cgatcgtttc
    17701 atggagcgcg tgccgcgtgg ggaacatccc gtcgaccacc gtcgaacggc cgtcgaacgt
    17761 gtagttcgag tgcagctcga gcatggtgtg atggggatcc accagatcgg cgccgtagac
    17821 ctggatttcg tagttgtctt gcgccgcgag gggagccgca gccagaacga gcgccagcaa
    17881 ggtgatggtc ttcacggtgt caaagatacg gcgggccggg gtgggccgtt gtagtattgc
    17941 cccatggcga ttctcatttc ccccacccgt ggccgcaacg ccgccatcct ggccggattc
    18001 ctcggctgga ccctcgacgc cttcgactac ttcctcgtcg tcctgacgct caccgccatc
    18061 ggtcaggagt tccaccggtc cgatgccgcg attggattct cgctcctcct caccctggcc
    18121 ttccgcccgg tcggcgcgct cgtcttcgga ttgctctcag accggttcgg ccgccggctc
    18181 cccttgatga tcaacctcgt gttctacgcc gtgatcgaag tgctcaccgg gttctcgacg
    18241 agctacggca tgttcctggt ccttcgcgcg ctcttcggca tcggcatggg tggagaatgg
    18301 ggggtggggg cttcactggt aatggaaaaa gtgtcgccga agtggagagg gttcttctcg
    18361 ggcttgttgc agcaaggata cgcgctgggt tacctcctcg cggccctcgc ctactggtac
    18421 gtcttccccc gctggggttg gagacccctg ttcttcatcg gtgggttacc cgccctgctg
    18481 gcgatcttcg tacgggccca ggtgtcggag tcggaggtct ggctcaagac gagagaaccg
    18541 acatggggcg gcctcggccg caccctcaag agccagatcc cgctcttcct ctatctcacc
    18601 gtgctcatgg cgctgatgaa catgtcgtcg cacggcaccc aggatctgta tcccacgttt
    18661 ctcaagcgcg cgtggcagct cactccccgt ggagtttcgt gggtcacaat cgtatcgatg
    18721 atcggtgcct tgatcggagg catcgtcttc ggtcactggt ccgagcggct gggacgccgc
    18781 aagaccatca cgctgacgct tctgggtgcc gtcctggtcg tcccactctg ggccttcgcc
    18841 ccgtccctgg ccttgctggt ggttggcgcc ttcctgatgc agttcctggt gcagggcgcc
    18901 tggggcgtca tcccggcgca catcaccgag ctcgtgcccg atcaggtgcg gggtgccctg
    18961 cccggcttcg cttaccagtg cggggcgctg atctcgggag cgatgccctt cctgcagccg
    19021 aaggtggcgg agaccctggg gtaccccgcc accatggctc tttctgccgg agccgcattt
    19081 ctcatatgcg cacttgtgac gtctctggga aaggagaagc gcggcgtcgt ctacggcgct
    19141 tagggctaca ttttcatggg cgttgccgtc aacgcccgct ccatcgctgc gcggacaaag
    19201 acccgcgttt ccttctttat cgcgtcgcgc agcttggcct tccccgcctc cccaccgact
    19261 gctccgacca cccgcaccgc agtgaccgcg aagagcgggt cgtagtccgg gatcaagcgc
    19321 agggcgaccg ccgtggcgcg cgccgagtca ccggtcgcgg cgagggcctg caaggcgacc
    19381 tgccggatat tgcgatcttc ggttgtcgcg gtattgcgct cgagcgcgtc ttcggccttg
    19441 ccatcccgga tgcggctgag gaaccccatg gcggtgaagc gcatgttggc gatctgatcg
    19501 ggaccaaccg ccgcaagcaa cgtcggcagc gcgtctttcc ccttctgcga cgcgatcacc
    19561 gaaagcgcta ccgcccggac gtcgtcgttc gggtcggtgg tgtacatcgc gttggcaacc
    19621 gtcagcgcct gcgcggcgtc gagggaatcg agcgattgca gcgcctgcat ccggacctgt
    19681 ggctcgggat cacggagagc agacgtcagg acccccttcg actccgggtt cgcgtcgtgg
    19741 gccatcatcc gcgtcgcgac ctcccgcaac cactccgtat ggtcgttcag caccaggaat
    19801 cggcgggtct cgacggccgc cggatccatg ttgccctcga gctgacccag agcccaacgc
    19861 cggccccgga tatccagatc gtgcttggcc tcatcggcca gctcggcgac cgacaaatca
    19921 cccttcacgg tgcccaggag ccagccacct tcatcgaacc ggaatgaaag cggctcggcc
    19981 ggcagcgcga tggcgaaggt gtcggtctgg tgatgtacga agatctgctg tcggatcacg
    20041 gaatctcgtg tgatcacacg gatcgttgcc gggaactgga agaccgggtg gagggagtcg
    20101 atcggctggg tctgcgagac cacgacgtgg agcgtcttga gattcgcggc ccagtgccgg
    20161 tggaactcga ccttggggta cccaattccg tacgcccact ggtcgaagaa ccagtcgaga
    20221 tcgcaattgc aggtcttttc catcgcgatg gcgtaatctg ccgtcgtcac gggcttgtac
    20281 gcgttgtcga cgaggaagcg gtgcatgccg gcccagaata cggagtcccc caggagtcgc
    20341 cgcaactgat gcgcgagctg ggcgcccttc ggatagacat gacctgagaa gaagagcgcg
    20401 ataggatcag tgccctggta tttcccccag accagagacc gcacctcgtt ctggtcggcg
    20461 cccatcgcct gttgctgttg tccgtaccag ttcagctctg cgtcatccca tccacgggtc
    20521 ttctcgtcct gcaccgactc catgtaggtc gtaatgcctt cattcagcca gatgtccgcc
    20581 cagtcggcgg tggtggtcag gtcaccgaac cactgatgcg ccagctcgtg tgcgttcagg
    20641 ctgcgcgcgg agttttccgg ctcctgcgac accgaatgga gcgccagatc cgtctgcgtc
    20701 gtggccgaga cgttctccat cccgccgtag gtgaagtcgg gaatgatgga ttgatcgtac
    20761 ttgtcccacg cgaaattgac gccgaggatc tgcgaataga gctcgatcat ggagggggtt
    20821 tcaccgaagg cgcgccacgc cgcgttcacg gtgtcggcgt aggtccaata ctcgaccgga
    20881 atgccacgcc actgatcctt cagtactgtg aacggcccaa ccacgaccga atacaggtag
    20941 gtcgacgcag gcttttcctg cgaccagtgc cagatgttcg ccttgccgtc cttcgtgggc
    21001 gtcacgccga tcagtttgcc gttcgacaga accttcaaac cggtgtccgc cgtaaccaga
    21061 aactcccagg tcgtcttgtc attggacgcg tcgtacgtgg gaatccacgc ccgcgtctcc
    21121 gtcgcctccc cctggctcca cacgatgttc ttgcggggga cgatgtagag cccgcgttca
    21181 ggcacggtgt gataggtcag cgagaactgg accgtgtccc cgaccgcggc gcggtgcgcc
    21241 agcttcaccg tcacgtgtgc cgtgtcctgc gtgaaccgca acgtgcggcc atggtcatcc
    21301 gacgcaccgt cgatcgtcag gttctcggcg ttcaggaaaa tcgtgtcggt gggcgcgagg
    21361 gtaatcgcga cgcgggtcgt gaccgtcgcg aacagctcgc gatgcggcac gtcgaaccgc
    21421 atctcgatct tctggtggag caggtcgaac cgccgcgcct cgggcttcca cactctcggg
    21481 gccgtgtact ggccgatcac gcccgtctgt cgttgggccg cgagcagcat cgggatcgtc
    21541 aaggcggcgg acaacatcga catcatgcga ttgcgcacgg attgcctcgg gtaggtgtca
    21601 gggtccaggg gtcagggaac tgctcttgcg tccattcgcg ccaggctgtt tcatcccagc
    21661 caatgttaga tatccaaatt gaggcttgcg ttgactcagt ggaatccgcc gtccaggccc
    21721 aagccggggg cgcgcaccgc gtggagctct gtgacgccct ggacgtcggg gg
//
LOCUS       DASUBP010000209         8399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_646739, whole genome shotgun sequence.
ACCESSION   DASUBP010000209 DASUBP010000000
VERSION     DASUBP010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 8399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8399
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_646739"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="VFU75_08050"
     CDS             complement(<1..690)
                     /locus_tag="VFU75_08050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466828.1"
                     /translation="MKALIPVLLSLTAFSQGLQAQRDSIRLDSATYARWSQARLSAVL
                     KAVDSAEAAFHAHYGRYTMVSDSLGLERSVGGTWSAVLWADSTGWRAVAANWGVADGA
                     CVVSGGSFEPVTGTDSLPPGIRCTVKDAPTWVPSEAVIDSVAADRSIGRVKHHQEKPN
                     EECPPIQLTPDGLQALRRNPQHLVVDFLIDSTGEPEVRRLRVTGGTEFNGPEGAMETL
                     AKCHFRPARIDS"
     gene            complement(1076..1453)
                     /locus_tag="VFU75_08055"
     CDS             complement(1076..1453)
                     /locus_tag="VFU75_08055"
                     /inference="COORDINATES: protein motif:HMM:NF024953.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HEU5466829.1"
                     /translation="MPSEAGPEEVVIVWSLRIAVAAFFVWTGFEKFSDSATSQWVGIF
                     HRIGLGDWFRYFTGVVEVGGGALFLIPRTTWIAGAGLGATMLGATIAHLTVLGDPGSS
                     AITIMILLGVVGVAARVSERRAA"
     gene            2137..5262
                     /locus_tag="VFU75_08060"
     CDS             2137..5262
                     /locus_tag="VFU75_08060"
                     /inference="COORDINATES: protein motif:HMM:NF024371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 domain-containing protein"
                     /protein_id="HEU5466830.1"
                     /translation="MQSFTPLFCSLLVSLSTVSAVPAAAQGSSSSQYADQGFIISSRS
                     LVIRMAPDGTGRIDLRARIAIQSQAGVAALGELKFDYDSSTEHFDVDSVEVLKRGAAP
                     VIATSVQDVTGPLGQEVPTYSDLRQKVVTVPALDPGDTIAYHITWTIDHPIAPGQFWY
                     YAPFLHQVTIKSEDDEVVVPVAVYTQVSTRGDVTPAVADSAGWRVYRWRRANLTPDTT
                     RTTLQKREGIEITTFRSWNDLGAWYGGLERPRETVTPAIQAKARDLVQGRRTLTDSIA
                     ALFTFVSDHIRYVALNFGVGRFQPHAASDVLANEYGDCKDKHVLLAALLHAIGVPSAP
                     VLINTERDADSAVVSPSEFDHLITVVPLAKDTLWLDATPSVAPFQFLWYELRGRRALV
                     IPASGPARLSRAPRTLPFAPTEDVTMRGGLTAATIALGVRYSERGDGEVLLREVFRTL
                     PRSSWPAMAARLDDNLPGTGITSDLDAARPEDTDSAFWMTFRLTQSGALTWKDGRAGY
                     QPPLPAMSFGLDDSTADTAHIDVLIHQSRRLVLGLPAGVSANLPLPVSITRDYADYRS
                     TYTLRGDSVEVVRTLDMKAPFVLKSRRSDFQAFRRTVRNDEAQLIGLIGTPAAVAATP
                     VPKADADELVRRGNNSLQSGDGRGAADLFGQAIAADPKNPYAWNDLGRAYLDLRMLDS
                     AAHAFRQQIAINPFDQYAYNNLGLAQWRSGDLTSAAASFRKEIEVNPLDQYAHSNLGQ
                     VDLLLKADSEAARELAKAAVITPGDAYVHANWGRALARIHQGDSAVTEFDRAIQMAPN
                     QWYIDHQAAAALSESGLRLDKAEEYAHAELAHATTAVQHAGETPGGFDGATLNVGFAW
                     DQVGWILFREGKTAEAERYEKAAWALTRHGPIGDHLGQVYQREGKRSEAIRTYALVLN
                     AHATPPETRRRLATLVGSDRMADQFADSGRMWLVNERSIDLHRGGPEGTAMLRILTNL
                     HGVTKVDVTNGSPTFSALASSIERAHLPITFPDTVSLTLPLRAVVSCSSERCLLTLTE
                     PSVVTMPLNTQ"
     gene            5379..7826
                     /locus_tag="VFU75_08065"
     CDS             5379..7826
                     /locus_tag="VFU75_08065"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5466831.1"
                     /translation="MPFDLRYVLRRIARFPGGAIVIVLSLGAGMAAVAAMLGVVDTLF
                     FRPPAGVSQPDRVVGVMAWKAHDQRATYPDYLDLQREATSYSSVGAFAPVTYSARIGK
                     TAVPAQGMLATSSLLPLLGVQSVLGRGFTTSEDQPGAPRVALVNPEFWKRAMGGDVPA
                     LGATIYLADQPFTIIGVLPARFTAPDLSPVDLLLPITNSPWFGGAEALRSRNYSRWLR
                     IIGRLKPGIDLPAAQAEATAIYRRSNAGVPSVDQAALPHVVIPVRPIIQARQDPTAPV
                     TRVSVWVTLLAAAVLLIACANVATLLLARGVRERHALAIHAALGAPRRRVIGRVVLEL
                     VVLVSASALLALVLTRAASDAISSLLLANTVAPPALDLRMAGTVLSVALVTALFCAIA
                     PVSRAWRLDPRTELATGASTSTRTHRFAFRLLLVGQSALCFVLVTEAILFAASLRNAT
                     RTDLGIKLDHLLIADADLHAAGLSGAGALAAVRRGLAGVSAIPGVRAAGVTDAAALPP
                     YLTFTISIPGADSASQAAGPPPSLVNGITAGYLDALGLPLIAGRMLTDEDIAGGRPVA
                     LISEGLARRFWPRATPLGACVKIGGPSGGCSEVVGVVGDRHAGPGDVAPDEEVYVPLG
                     SPALPQGLASIFPGREVAVRVTGDVGRVESALRTALLDAVPQLTSVRVRTGEDYLERQ
                     FRSWRLGAGILSAFGVVALALAAVGVFGVSACVVDQRRRELGIRGALGARPTQLASLV
                     LSESIWVNAAGIGLGVGGAFALARMVRTLAFGVSPLDPGLLGVVALVLVTTTLLSALW
                     PSVRAATVDPTTALRNE"
     gene            7938..>8399
                     /locus_tag="VFU75_08070"
     CDS             7938..>8399
                     /locus_tag="VFU75_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466832.1"
                     /translation="MEHLTASLRLIAALVLSAHHPRSAATHLTVAVDSAHREVILSAG
                     PFIIPTQNTSDMSAMDMDQMMRNGETEAGRFTWPVSGLYRSVELEVLDSTGHPLPRRL
                     LHHLNLINFDRRQLVYPLTERFIGFGQETEDVALPKSLGLPLDHGQRIGLYL"
BASE COUNT         1385 a         2986 c         2608 g         1420 t
ORIGIN      
        1 actgtcgatg cgggccggcc ggaagtgaca tttcgccagc gtttccatcg ccccttcggg
       61 gccgttgaac tcagtgcccc cggtcacccg tagtcgtcgc acttcgggtt caccggtcga
      121 atcgataaga aaatccacta caaggtgctg ggggttccgg cgcagggcct gcagcccatc
      181 aggggtcagt tgtatcggcg ggcactcttc attgggcttc tcctggtgat gcttcactcg
      241 tccgatcgac cggtcggccg ccactgagtc aatcacggcc tcggacggca cccacgttgg
      301 ggcgtccttg actgtgcagc gtatgccggg agggagcgag tccgtcccgg ttaccggttc
      361 gaagctcccg ccggagacga cgcacgcccc atccgcaact ccccaattcg cggcgacggc
      421 gcgccatccg gtcgaatccg cccacagcac ggcgctccag gttccgccga cggagcgctc
      481 gaggccaagc gagtcggaca ccatggtgta gcgtccgtag tgagcatgaa acgccgcctc
      541 cgcgctatcg accgccttca acacggcgct caggcgcgcc tgactccagc gggcgtaggt
      601 cgccgaatcg agacggatcg agtcgcgttg cgcctgcagc ccctgactga aggccgtaag
      661 agaaagcagc accggaatca gtgccttcac tcgtcgctca cctcaatttg aggtttctgg
      721 cgctcgttca ttttgagcag tatacgctac gtcagtgatc gtgccagtcc cgagtggtca
      781 cagggctttt gcgaccagcc cttcggcccg caggccgtgg tcacaccagt ggtcacaagt
      841 ggtcacagct tcggaccttc ggcttttcgg gccggggtca caacaatggt cacagagaat
      901 gcgtcgttgg tcgttgaagg tcatcgacgc gattcgctaa cccgcgatac agcaacggcc
      961 agcgctggag tgcgctggcc gtgctggtct ccggagccga aggtcgtggg ttcgaatccc
     1021 gcctggcgca ttccccgcat cgatcctgac gtgcaccccg agatcagtct tccctctacg
     1081 ccgcccgccg ttcgctcacc cgtgccgcca caccgaccac acccagcaga atcatgatgg
     1141 tgatcgcgct cgagcccgga tcccccagca cggtcaaatg ggcaatcgtg gcgccgagca
     1201 tcgtggcgcc gagtcccgca cccgcgatcc aggtggtgcg cgggatgaga aagagggcac
     1261 cgcccccgac ctccacgacc cccgtgaaat accggaacca gtcccccagt ccgatgcgat
     1321 ggaaaatccc cacccactgg gacgtcgcgc tgtccgagaa cttctcgaac ccggtccaga
     1381 caaaaaaggc ggccaccgcg atccgtagac tccacacaat cacaacctct tcgggccccg
     1441 cctcggaggg catccgactc acttgcgcgt ctcctttccg tttcacgtcc tcacttcatc
     1501 accgagtgtg ggcacgggat cgaggatagc gtgaccagcc gtgtcctcgt cgtggcagtc
     1561 gtgacgacct cgaagaagtt gtcgtagtag gcctcgaata gacgtccaat gaggcggatc
     1621 gagtccggcc aataccaatg gttctagtag gtgaggactt tgaagcaccg tacgagcgaa
     1681 ccagccgcaa gcgcctcgct gcccgcggga agtgcggtct ccacaacgac aggaaatacg
     1741 agcccaatgt ccaccaatag agtagcgaga atcgccatga atacgccgag acgcacgcgg
     1801 tccccctgtg gggctcacgt ccatggtaca acccgcgaat gattggccag tgtgggacag
     1861 attacgagtc gatgacagac gctcataccc tttgacctcg tcagccgata cctgccctga
     1921 tcgagccagt agccattcac accgggttgc ttcgggagag agtcgacccg tggtcgttcc
     1981 cgcgggctag gagccgctaa agcacaggcc gctctcggcg cgggcgcggc ctgaaggcct
     2041 tcagaatggc aatcggtccg cggggtggac acatgtgtag aagcgcgtaa actgaacgga
     2101 tccgtttcaa gccgtcattc cgtccaggag catcacatgc agagcttcac gccgttgttc
     2161 tgcagcttgc tcgtctccct ctcgaccgtc tcggcggtcc cggccgccgc ccaggggtcg
     2221 tcatcatcgc agtacgcgga ccagggcttc atcatctcca gccgcagcct cgtgattcgg
     2281 atggcccccg acggaacggg gcgcatcgac ctgcgggccc gcatcgccat tcagtcccag
     2341 gcaggggtag cggcgttggg ggagctcaag ttcgactacg actcttccac cgagcacttc
     2401 gacgtggact ccgtggaggt cctgaagcgg ggggcggccc ccgtgatcgc aacctcggtc
     2461 caggacgtga ccggacccct gggacaggag gttcccacct attcggacct gcggcagaaa
     2521 gtcgtaaccg taccagcgct ggaccccgga gacacgatcg cctaccacat cacgtggacc
     2581 atcgatcacc cgatcgcccc gggacagttt tggtactacg cccccttcct gcaccaggtc
     2641 accatcaaga gcgaagacga cgaagtcgtg gttccggtgg ccgtctacac ccaggtgagc
     2701 acgcgcggcg acgtgacccc ggcagtggcg gacagcgcgg gctggcgcgt ctaccggtgg
     2761 cgccgcgcca atctcacgcc cgacacgaca cggacgacac tgcagaagcg cgagggcatc
     2821 gagatcacga cgttccgttc ctggaacgac ctgggcgcgt ggtatggcgg tctcgaacgc
     2881 ccacgcgaga ccgtgacccc ggccatccag gccaaggcgc gagacctggt ccagggccgg
     2941 cgcaccctga cggactcgat cgccgccctg ttcacgttcg tctccgacca catccggtac
     3001 gtcgcgctca acttcggcgt cgggcggttc caaccgcacg cggcatcgga cgtgctggcc
     3061 aacgagtacg gtgactgcaa ggacaaacac gtcctgctgg cagcgctcct ccacgccatc
     3121 ggcgtcccca gcgctccggt cttgatcaac accgagcggg acgccgactc ggcggtggtc
     3181 tcgccatctg aattcgacca tctcatcacc gtagtcccac tggcgaagga cacgctctgg
     3241 ctcgacgcca ctccctccgt cgcgccgttc cagttcttgt ggtacgagct gcggggccga
     3301 cgggcgctcg tgatccccgc gagcggtccg gcccgcctga gccgggcgcc tcgcaccctc
     3361 ccgttcgcgc cgaccgaaga cgtcaccatg cgcggcggat tgacggccgc cactatcgcg
     3421 cttggtgtcc gatacagtga gcgcggcgat ggggaggtcc tcctgcggga ggtgttccgc
     3481 accctgcccc gcagctcgtg gccggccatg gctgcccggc tcgacgacaa cctccccggc
     3541 accggcatca ccagcgatct cgatgcggcc cggccggagg acaccgacag cgcgttctgg
     3601 atgaccttcc ggctcacgca atcgggcgcg ctcacctgga aggacggccg ggcgggctac
     3661 cagccgccgc tgccggccat gtcattcggt ctcgacgact ccaccgcgga cacggcgcac
     3721 atcgacgtcc tgatccatca gtccaggcga ctggtgctgg gcctcccggc aggggtctcg
     3781 gccaacctcc cgctcccagt ctccatcacc cgggattacg cggactaccg gtcgacgtac
     3841 acgctccgcg gggacagcgt ggaggtcgtg cggacgctcg acatgaaggc gcccttcgtc
     3901 ctcaagtccc gccggagtga cttccaggcg ttccgccgca ccgtccggaa cgacgaagcc
     3961 caactcatcg gcctgatcgg cacccctgca gccgtcgccg cgacaccggt gcccaaggcg
     4021 gacgccgacg agctggtgcg ccgtgggaac aacagcctgc aatcggggga cgggcgcgga
     4081 gccgccgatc tcttcggtca ggccatcgcc gccgacccga agaatccgta cgcctggaac
     4141 gatctggggc gcgcgtatct ggacctgcgg atgctcgatt ccgccgccca cgcctttcga
     4201 cagcagatcg cgatcaaccc gttcgaccag tacgcctaca acaacctcgg cctggcccag
     4261 tggcgatccg gtgacctgac cagtgccgct gcgagctttc gcaaggagat cgaggtcaac
     4321 ccgctcgatc agtatgccca ttcgaatctg ggacaggtgg acttgctgtt gaaagccgac
     4381 tccgaggccg cacgggaact ggccaaggcc gcggtcatca ccccgggcga cgcttacgtg
     4441 cacgcaaact gggggagggc cttggcacgg attcaccagg gcgacagcgc ggtcaccgag
     4501 ttcgaccgcg ccatccagat ggctccgaac cagtggtata tcgatcatca ggcggcggcc
     4561 gcactctcgg agagcggcct gcggctcgac aaggcagaag agtacgcgca cgcggagctg
     4621 gcccatgcca ccaccgcagt ccagcacgcg ggggaaaccc cgggtggctt cgacggggcg
     4681 acgctgaacg tgggctttgc gtgggaccag gtgggctgga tcttgttccg cgaggggaaa
     4741 accgcggaag ccgagcggta cgagaaggcg gcctgggccc tgacccgcca cgggccgatc
     4801 ggggaccatt tgggacaggt ctaccagcgt gagggtaagc gctccgaggc aatccgtacc
     4861 tacgcgctgg tcctcaacgc ccatgccacg cccccggaga cccgccgccg gctggcgacc
     4921 ctggtgggga gtgaccgaat ggctgaccag ttcgcggact cgggacgcat gtggctggtg
     4981 aacgaacgca gcatcgacct gcatcggggc ggcccggaag ggaccgcgat gctccggatt
     5041 ctcaccaacc tccacggcgt caccaaggtc gacgtgacca acggatcgcc caccttcagc
     5101 gcgctggcat cgtcgatcga gcgggcacac ctgcccatca cgttccccga caccgtctcc
     5161 ttgacgcttc ccttgcgggc cgtggtcagc tgctcctcag aacggtgcct gctgacgttg
     5221 accgagccct cggtggtcac catgcccctc aacactcagt agcggccatg accgcgaccc
     5281 ccgtcgttcc cgcgggctga gagcccgctc tcccctctca gggcgcggga aacgacgacc
     5341 ttgtcgacgc cgctcgcttt gtgcagtata gctaacccat gcccttcgac ctccgctacg
     5401 tcctccgccg catcgcccgc ttccccggcg gggcgatcgt catcgtactc tcgctcggcg
     5461 cgggcatggc cgcggtcgcg gcgatgctcg gtgtggtcga cacgctcttc ttccgcccgc
     5521 ccgccggcgt gagccaaccc gaccgcgtgg tcggagtcat ggcctggaag gcccacgatc
     5581 agcgcgccac ctaccccgac tacctggacc tgcaacggga ggccacgagc tactcgagtg
     5641 tcggagcctt cgcaccggtg acctattccg cccggatcgg gaagaccgcg gtccccgccc
     5701 agggaatgtt ggccacgagc tcactcctgc ccttgctcgg cgtgcagtcg gtcctggggc
     5761 gagggttcac gacatcggag gaccagccgg gagcgccccg ggtggcactg gtcaatccag
     5821 agttctggaa gcgcgccatg ggtggcgacg tccccgcact cggagcgacg atctaccttg
     5881 ccgatcagcc cttcaccatc atcggcgtac tgccggcacg cttcacggct cccgacctct
     5941 cccccgtcga cctgctgctc ccgatcacca actccccgtg gttcggaggc gccgaggcgc
     6001 tccggagccg gaactattcg cgctggctca ggatcatcgg acggttgaag ccaggaatcg
     6061 acctgcccgc ggcgcaagcc gaagcgacgg cgatctatcg ccgctccaac gcaggcgtgc
     6121 cttccgtgga tcaggccgcc ctcccgcacg tcgtcattcc ggtccgccca atcatccagg
     6181 cccggcagga cccgaccgcc ccggtcaccc gcgtatcggt ctgggtcacg ttgctcgcag
     6241 cggccgttct gctcatcgcg tgcgcgaacg tcgccaccct gctcctcgcg cgcggcgtcc
     6301 gggagcgcca cgcgctcgcc atccacgcag ccctcggcgc tccacgacgg cgcgtcatcg
     6361 gccgggtggt cctcgaactc gtcgtgctgg tgtccgcttc cgcactgctg gctctagtcc
     6421 tgactcgcgc ggccagcgac gccatctctt ccctgctcct cgcgaacacc gtcgccccac
     6481 ctgccctcga tctgcggatg gcgggcaccg tgttgagcgt cgccctggtc acggcgctgt
     6541 tctgtgccat cgcaccggtc agccgagcct ggcggttgga cccacggacc gagctcgcaa
     6601 cgggcgcttc gacgtccacc cgaacgcatc gcttcgcgtt ccggctcctg ctggttggcc
     6661 agtccgctct ctgcttcgtt ctcgtgaccg aggcgatcct cttcgccgcg agcctccgga
     6721 atgcgacccg gaccgatctg gggatcaagc tcgaccatct cctcattgcc gacgccgacc
     6781 tgcacgcggc gggtctctca ggagcagggg cgcttgcggc agtgcgccgt ggactagcgg
     6841 gcgtctcggc gatcccgggt gtgcgcgcgg cgggagtgac ggatgccgca gcgttaccgc
     6901 cctacctgac gttcacgatc tcgatacctg gtgcggactc ggcgtctcaa gccgcgggcc
     6961 cgccaccgtc gctggtcaac ggcatcaccg ccggctacct cgacgccctg ggcctgcctc
     7021 tgatcgcggg ccgaatgctg acagacgaag acatcgcggg cggccggccg gttgcgctca
     7081 tcagcgaggg cctggcgcgg cggttctggc cccgcgcgac ccccctcggc gcgtgcgtga
     7141 agatcggcgg cccgtcggga ggctgctcag aggtcgtagg cgtggtcggt gaccggcacg
     7201 caggaccggg cgacgtcgcc ccggatgaag aggtctatgt ccccctggga tcacccgcgt
     7261 tgccgcaagg attggcttcg atttttcctg gtcgagaagt cgcggtccgg gtcaccggcg
     7321 acgtcggtcg ggtcgagtcg gcgctccgga cagctctact ggacgccgtc cctcagctca
     7381 catcggtgcg ggtcagaact ggcgaggact acctggagcg gcagttccgc tcctggcgtc
     7441 tgggagcggg catcctgagc gcgttcgggg tcgtcgcatt ggcgctcgcg gccgtgggcg
     7501 tgttcggagt gtcagcgtgc gtggtggatc agcggcggcg ggagctcggt atccgcggcg
     7561 cactcggagc acgtcccacc cagctcgcgt ccctcgtgct ctccgagtcg atctgggtca
     7621 acgccgccgg gataggcttg ggagtcggcg gcgcattcgc gctggcccgc atggttcgga
     7681 cgcttgcgtt cggcgtgtca cccctcgatc caggcttgct gggcgtcgtc gcgttggtcc
     7741 tcgtgacgac aaccctcctc agtgccctct ggccgtcggt gcgcgcagcc accgtcgacc
     7801 caaccaccgc cttacgaaac gagtgaccat cgtcgtcaca ctcccccttc attccatctt
     7861 cacgattccg gatcactccg gggtatgcga gtcgccgagt agaccccggc gcgtaggttc
     7921 caccgcctcc ccaacgaatg gagcacttga ccgccagcct ccggttgatc gccgcgctcg
     7981 tcctctcggc ccatcacccc cggtccgccg ccactcactt gaccgtcgcg gtcgattcgg
     8041 cgcaccgcga agtcatcctc tccgccgggc cgttcatcat tccaacccag aacacgtcgg
     8101 acatgtcggc gatggacatg gaccagatga tgcggaacgg tgagacggag gcggggcgct
     8161 tcacttggcc cgtctcgggg ctctaccgct ccgtcgagtt ggaagtcctc gactccaccg
     8221 gacacccgct gccccgccgg ctcttgcacc acctcaacct catcaacttc gatcgccggc
     8281 agctcgtgta ccccctcacc gaacggttca tcggattcgg ccaggagacg gaggacgtcg
     8341 cgctccccaa gtccctgggg ctccccctcg atcacggcca gcggatcggg ctctacctg
//
LOCUS       DASUBP010000210         6826 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_837775, whole genome shotgun sequence.
ACCESSION   DASUBP010000210 DASUBP010000000
VERSION     DASUBP010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6826)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6826)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6826
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_837775"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(64..1962)
                     /gene="dxs"
                     /locus_tag="VFU75_08075"
     CDS             complement(64..1962)
                     /gene="dxs"
                     /locus_tag="VFU75_08075"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003933.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEU5466833.1"
                     /translation="MNLLDQIHGPDDVKRLPPESLQQLADDVRQRLIEVVSQTGGHIG
                     AGLGVVELTVALCRAFDSPKDKIVWDVGHQGYPWKILTGRNDRFHTLRQPGGLNGFLR
                     RSESPHDQFGAGHAGTALSAAFGMAAARDLAGDHYRVVAVVGDGAMTCGLPYEALNNA
                     GHSNRDIIFVLNDNGMSIAPNVGAMSKYLGSMIANPLTVRVREIAKGMIERASHLVGG
                     PKLVEFAKTVEESVKNLWSPGMLFEELGFRYFGPIDGHNIDQMLSTFEIVKSLKGPRV
                     VHVLTHKGKGFPHPEPDIEKFHARAPYDPVTGKLKPVAPGAPQWTKVFGDALVQLGAE
                     YPHLVAITAAMPSGTGTNLFQKAYPDRFFDVGIAEGHATTFAAGMATQGIRPIVAIYS
                     TFLQRAYDNIIHDVAIQKLPVIFAMDRAGMVGDDGQTHMGLYDIAYLLAVPGMTVTAP
                     RDGKELVGLLRCALQHDGPFSVRYPRDKAPGDVVPASEIQAVPYGSWEVLRKGKDGAI
                     LAVGVMCQPALAAAEQLAADGWDLTVVNCRFLKPLDRVTLDALVHEHQLLLTVEDGTV
                     VNGFGAYLAGVVETTAPGVRVVPLGVPDRIYEHAPRAQQLAEVGLTAEGIVTRFRALA
                     AEESLTPK"
     gene            complement(1978..2907)
                     /locus_tag="VFU75_08080"
     CDS             complement(1978..2907)
                     /locus_tag="VFU75_08080"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEU5466834.1"
                     /translation="MTTSTAETAPRGLDAYLIDCRQRVDAVLAEWSEEGARFWTAPVA
                     AAVRYSLLSPGKRLRPTLVFSAYESLGGGEAPIAELAAAVEVVHAYSLVHDDLPSMDN
                     DDLRRGRPTTHRAFDVPTATRAGYHMVALAARVLARGMVRLGLSPERQRDIALELFRA
                     AGAGGMIGGQALDLEGEGGGLDVAGLREVHARKTGALIAASCVIGALAAGEDVPPQRV
                     EALRGYGESIGLAFQIADDVLDATATSDQLGKTAGKDEAQDKTTFVTLLGVGAARAEA
                     ERLSAAAVARLLSAGLVSPTLVELAHYIVTRPN"
     gene            complement(2888..3112)
                     /gene="xseB"
                     /locus_tag="VFU75_08085"
     CDS             complement(2888..3112)
                     /gene="xseB"
                     /locus_tag="VFU75_08085"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR01280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEU5466835.1"
                     /translation="MATQPDFGKELERLEAIVRQLETPDLDLDQALKLFEEGVERLRA
                     ARDRLTAAQAKVKQVISDHAGTLKVDDLDG"
     gene            complement(3112..4245)
                     /gene="xseA"
                     /locus_tag="VFU75_08090"
     CDS             complement(3112..4245)
                     /gene="xseA"
                     /locus_tag="VFU75_08090"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEU5466836.1"
                     /translation="MFAAVAPDGAWSVSAVNRRAKAAIEQVLEPMWVEGEMTNLKDFG
                     GNWYFSLRDSLSQLRCVMFRDDARRLPAAPTEGMKVYAFGRATIYEKRGEYQLNVTRM
                     MSTEAGGLFRIAFEKAKAALAKDGILDRARKPIPTYPRWIAVVTSVDGAALRDIMTVA
                     GARWPVAQIAVIHTRVQGEGAVDEVVRALQLIQRLTKVDVAIVGRGGGSKEDLQVFNE
                     ERVARALAAVPVPVISAVGHETDVTLCDLVADRRAPTPSAAAEMATPDRDEVMSDLEH
                     LGARLARGLAHRVDRMDERLGRTSDRLVGAIERRMEAVSYQLSAVSGRLDALSPLGVL
                     ARGFAVPRDGTGRVLRRTADFHPGDPFDLRVVDGTVKARVEGN"
     gene            complement(4248..5144)
                     /gene="folD"
                     /locus_tag="VFU75_08095"
     CDS             complement(4248..5144)
                     /gene="folD"
                     /locus_tag="VFU75_08095"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HEU5466837.1"
                     /translation="MEAQLIDGTAIGKAMRAELAGDIEGLAKKGVKPGLAVVLVGENP
                     ASQVYVRMKGKACEEAGMHSETIRLPAETSEADLLAVVDRLNADPRIHGMLVQLPLPK
                     HINSERVLLRIDPAKDVDGFHPVNVGKVSIGDPTGFRPATPFGVQKLLLRTNVETKGA
                     HAVIVGRSNIVGRPMAALLLQNGPGGNATVTVCHSNTRDIASVTRQADILIVAIGKPE
                     FVTGNMVKPGAVVIDVGVNRVDDPSSKNGYRLVGDVQFAEAKKVASAITPVPGGVGPM
                     TITMLLYNTVQAAQQWTVPATR"
     gene            complement(5245..6759)
                     /gene="rny"
                     /locus_tag="VFU75_08100"
     CDS             complement(5245..6759)
                     /gene="rny"
                     /locus_tag="VFU75_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEU5466838.1"
                     /translation="MPGWASLFIAVLGGAVAAGAVFYVIGRRFERRIAALTSFNSERE
                     HARQLDEAKQARALEVKEELLRAREGLERELMGRRQEVERRQQTLDGKVTEIENRQRN
                     IGRLEEALKEREKGLAGLEAEGRARLERVAGLSAADAKQELVRALEDEARSDAAALVR
                     DIKEQAKKNADREARKIVAIAIQRIAAEQTAESTVAAVPLPSDEMKGRIIGREGRNIR
                     AFETATGVDVIIDDTPDTVVVSCFDPVRREVGRLALERLIADGRIHPGRIEEIVNKAR
                     EEIDAGIIEAGEQAAYDTGIHGLHPELIKLVGRMKYRTSYGQNILEHSKEVAWLAGMM
                     AAELKLDVPLSKRGALLHDVGKVMTHDHEGTHVQLGVEVATKYGEHPVVINCIAAHHD
                     DVPHESAISVIVQAADAISGSRPGARREAFETYVKRLGQLEDIASSYQGVEKVFAIQA
                     GREVRVVVTPNTVDDQGAVRLSQDIARKIESEVQYPGQIRVVVVRETRAVDVAK"
BASE COUNT         1026 a         2407 c         2230 g         1163 t
ORIGIN      
        1 ccgccagctg ggcgagcacc acgccaaggt cgcggtagtt ggcgttgccg acgaccccga
       61 cgttcatttg ggagtgagcg actcctcggc cgcgagcgcg cggaagcgcg tcacgattcc
      121 ctcagcggtg aggcccacct ccgcgagctg ctgcgcgcgc ggggcgtgct cgtagatccg
      181 gtcgggaaca ccgagcggaa ccacccgcac gccgggagcg gtcgtttcca ccacccccgc
      241 gagatacgcc ccaaagccgt tcaccacggt cccgtcttcc acggtcagga gcagctggtg
      301 ctcgtgcacc agcgcgtcga gcgtcacccg gtcgaggggc ttgaggaagc ggcagttcac
      361 gacggtgagg tcccagccat ccgcggcgag ctgctcggcg gcggccagcg cgggctggca
      421 cattacgccg accgcgagga tcgcgccgtc cttccccttc cgcaggactt cccacgagcc
      481 gtacggcacg gcctggattt cagacgccgg caccacgtcg ccgggagcct tgtcgcgcgg
      541 ataccggact gagaagggtc cgtcatgctg gagcgcgcag cgcaacagac ccaccagctc
      601 cttgccgtca cgcggggcgg tgaccgtcat cccgggcacc gccaggagat aggcgatgtc
      661 gtagagaccc atgtgggtct gtccgtcatc ccccaccatc ccggcgcggt ccatcgcgaa
      721 gatcaccggc aacttctgga tcgcgacgtc gtgaatgatg ttgtcgtacg cgcgctgcag
      781 gaacgtcgag tagatcgcca cgatgggcct gatcccctgc gtcgccatgc ccgccgcgaa
      841 cgtcgtcgcg tggccttcgg caatgccgac gtcgaagaac cggtccggat acgccttctg
      901 gaacaggttg gtcccggtgc cggacggcat cgccgccgta atcgccacca gatggggata
      961 ctcggcgccg agctggacca gcgcgtcacc gaagaccttg gtccactgcg gggccccggg
     1021 cgccaccggt ttgagcttcc cggtgaccgg atcgtagggc gcgcgggcgt ggaatttctc
     1081 gatgtcgggt tccgggtgcg ggaacccttt ccccttgtgc gtcaggacgt gcacgacccg
     1141 ggggcccttc aaggacttca cgatctcgaa cgtcgagagc atctgatcga tgttgtggcc
     1201 atcgatggga ccgaaatagc ggaagccgag ctcttcgaag agcatacccg gagaccagag
     1261 gttcttcacg ctctcttcga cggttttcgc gaattccacg agcttgggcc cgcccacgag
     1321 gtgggaggcc cgctcgatca ttcccttcgc gatttcgcgc acccggacgg tgagcggatt
     1381 cgcgatcatg gacccgaggt acttgctcat cgccccgacg ttcggcgcga tcgacatgcc
     1441 gttgtcgttc aggacgaaaa tgatgtcgcg attggaatgg ccggcgttgt tcagggcctc
     1501 atacggcaga ccgcacgtca tcgcgccgtc gccgacgacg gcgacgacac ggtagtggtc
     1561 ccccgccagg tcacgcgcgg ccgccatgcc gaacgccgcg gacagcgcgg ttcccgcgtg
     1621 ccccgcgccg aactggtcgt gcgggctctc actgcggcgg agaaatccgt ttagccctcc
     1681 gggttgccgc agggtgtgga agcggtcgtt gcggccggtg aggatcttcc agggataccc
     1741 ctggtgcccc acgtcccaga cgatcttgtc cttgggagag tcgaaggccc ggcagagcgc
     1801 cacggtgagc tcgacgacgc cgagtccggc accgatgtgg ccgccggtct gggagacgac
     1861 ttcgatgagg cgctgacgca cgtcgtccgc gagctgctgc agcgactcgg gcggcagccg
     1921 tttgacgtcg tcgggtccgt gaatctgatc gagcaggttc atctagccgt cagctctcta
     1981 gttgggccgg gtcacgatgt agtgcgccaa ctccaccagt gtaggggaaa ctaaccccgc
     2041 cgagagcaga cgggccaccg ccgcggcgga cagccgctcc gcctccgccc gggccgcccc
     2101 cacccccaga agggtcacga aggtggtctt gtcctgcgcc tcgtccttcc cggccgtctt
     2161 gcccagctgg tccgaggtcg ccgtggcatc caggacgtcg tcggcaatct ggaatgcgag
     2221 cccgatggat tcgccgtacc cccggagcgc ttcgacccgc tgggggggta cgtcctcacc
     2281 cgccgcgagc gcgccgatca cgcaggaggc ggcgatcagg gcaccggtct ttcgcgcgtg
     2341 cacctcgcgg agcccggcga cgtccaagcc gcccccttcg ccttccagat cgagcgcctg
     2401 tccgccgatc atccccccag ccccggcggc ccggaaaagc tcgagcgcga tgtcccgctg
     2461 acgttcgggg gagaggccga gccggaccat gccccgggcg agcacccggg cggcgagggc
     2521 taccatgtga tagccggcgc gcgtcgcggt gggcacgtcg aaggcccggt gcgtcgtggg
     2581 acggccgcga cgcagatcgt cattgtccat gctcggcaga tcgtcgtgca ccagggagta
     2641 cgcgtggacc acttcgacgg ccgcggcgag ctccgcgatg ggcgcctcgc ccccgccgag
     2701 tgactcgtag gcactgaaga ccagggtggg acgaagccgc ttgcccggag agagcagact
     2761 gtagcggacg gcggccgcca cgggagccgt ccagaacctg gccccctcct cgctccactc
     2821 ggccagcacc gcatcgaccc gttggcggca gtcgatcagg tacgcatcga gaccccgcgg
     2881 ggccgtttca gccgtcgagg tcgtcaacct tgagcgttcc ggcgtggtcg gagatcacct
     2941 gcttcacttt cgcctgagcg gcagtcaacc ggtcacgcgc ggcgcgcagg cgctcgacgc
     3001 cttcttcgaa gagcttgagg gcctgatcga gatcgagatc gggcgtttcg agctgccgca
     3061 cgatggcctc gagacgctcc agctcttttc cgaagtcggg ttgggtggcc atcagttccc
     3121 ttccacccgc gccttgacgg tgccatccac gacccgaagg tcgaatggat cgccggggtg
     3181 aaagtcggcg gtgcggcgca ggacgcgccc ggtcccgtct cgcggcaccg cgaacccgcg
     3241 cgccaacacg ccaaggggtg agagagcatc caggcgtccg ctgacggctg acagctgata
     3301 gctgacggcc tccatccggc gctcgatggc gccaacgagt cgatcggagg tgcgcccgag
     3361 ccgttcatcc atccggtcca cccgatgcgc cagcccccgg gccaggcgtg cgcccaggtg
     3421 ctcgaggtca ctcatcacct cgtcccggtc gggagtcgcc atctccgcgg cggccgaggg
     3481 cgtgggcgcc cggcggtcgg cgacgaggtc gcacaaggtg acgtcggttt cgtgacccac
     3541 cgccgaaatc accggcaccg gcacggcggc aagggcgcgc gccacccgct cttcgttgaa
     3601 gacctgcagg tcctccttgg acccgcctcc ccgccccacg atcgccacat cgaccttggt
     3661 gagccgctgg atcagctgga gcgcgcgcac gacctcgtcc acggccccct caccctgcac
     3721 ccgggtgtga atcaccgcga tctgggcgac cggccaccgc gcccccgcaa ccgtcatgat
     3781 gtcccgcaga gcggctccgt cgacgctcgt gaccacggcg atccagcggg gatacgtggg
     3841 gatcggcttg cgggcccggt cgaggatgcc gtccttggcc agcgcggcct tggccttttc
     3901 gaacgcgatc cggaagagcc cacccgcttc ggtggacatc atccgggtca cgttgagctg
     3961 atactcgccc cgtttttcgt agatggtggc gcggccaaag gcgtagacct tcatgccttc
     4021 ggtgggcgcg gcggggagcc ggcgcgcgtc gtcgcgaaac atcacgcacc ggagctgtga
     4081 caggctgtcc cgcagggaga agtaccagtt cccgccgaaa tccttgaggt tcgtcatctc
     4141 gccctcgacc cacatcggct cgaggacttg ttcgatggcc gccttggccc gccgattcac
     4201 cgccgacacg ctccaggcac catctggagc gacggccgca aacatgtcta ccgggtggcc
     4261 ggaacggtcc actgctgggc ggcctgcacg gtgttgtaga ggagcatggt gatcgtcatg
     4321 ggtccgacgc cgcccggcac gggcgtgatc gccgacgcga ccttcttcgc ttcggcgaac
     4381 tgcacgtccc cgaccagccg gtagccgttc ttgctcgagg ggtcatccac ccggttcaca
     4441 cccacgtcga tcaccaccgc ccccggcttc accatgttgc ccgtgacgaa ctccggtttg
     4501 ccgatggcga cgatcaggat gtcggcctgc cgcgtcaccg aggcgatgtc gcgggtgttc
     4561 gagtggcaga cggtgacggt ggcgttgccg ccgggaccat tttggaggag gagcgcggcc
     4621 atcggccggc cgacgatgtt ggagcgcccg acgatcacgg cgtgggcgcc cttcgtctcg
     4681 acgttggtgc gaaggaggag cttctggaca ccgaatggcg tcgcgggccg gaaccccgtc
     4741 gggtctccga tcgaaacctt gccgacgttc acggggtgaa agccgtcgac gtccttggcg
     4801 ggatcgatcc gcagcagcac gcgctccgaa ttgatgtgct tgggcagcgg cagctgcacg
     4861 agcatgccgt ggatccgggg gtcggcgttg agacgatcga ccaccgcgag gaggtcggcc
     4921 tcggaagtct cggcggggag ccggatggtc tcggagtgca tgcccgcttc ctcgcaggcc
     4981 ttgcccttca ttctcacgta gacctgggaa gcggggttct cccccaccag cacgaccgcg
     5041 agccccggct tcacaccctt cttcgcgagg ccctcgatgt ctccggcgag ctccgcgcgc
     5101 atcgcctttc cgatggccgt cccgtcaatg agctgcgctt ccacctgggt acctccctta
     5161 aagccctgac ccccgacccc tggcccccga cccctgaccc ctggcccccg acccctggcc
     5221 cctgacccct ggcccctgac ccctctactt cgctacgtcg accgcccgcg tctcccggac
     5281 caccacgacg cggatctgac ccggatattg cacttcactc tcgatcttcc gggcgatgtc
     5341 ctgcgagagc cgcacggcgc cctggtcatc caccgtgttg ggagtcacga cgacccgcac
     5401 ctcccggccc gcctggatcg cgaagacctt ctccacaccc tgataggagc tcgcgatgtc
     5461 ctcgagctgg cccagccgtt tgacgtaggt ctcgaacgct tcgcggcgcg cgccgggacg
     5521 ggaaccggag atcgcatccg cggcctggac gatcacgctg atggccgatt cgtgcggcac
     5581 gtcgtcatgg tgtgcggcga tgcagttgat gaccacggga tgctcgccgt acttggtcgc
     5641 cacctcgacg ccgagctgca cgtgcgtgcc ctcgtggtcg tgcgtcatga ctttgcccac
     5701 gtcatgcaac agcgcgccac gcttggaaag cggcacgtcg agcttcagct cggccgccat
     5761 catcccggcg agccacgcga cttccttcga gtgctcgagg atgttctggc cgtaggacgt
     5821 ccggtacttc atccggccca ccagcttgat cagctcgggg tggagaccgt ggatcccggt
     5881 gtcatacgcc gcctgctccc ccgcctcgat gataccggcg tcgatttctt cccgggcctt
     5941 gttgacgatc tcctcgatgc gccccggatg gatccggccg tcggcgatga ggcgttccag
     6001 ggccaggcgt cccacttcgc ggcggacggg gtcgaagcag gagaccacca ccgtatcggg
     6061 cgtgtcgtcg atgatgacgt cgacgccggt ggcggtttcg aaggcgcgga tgttccggcc
     6121 ttcgcggccg atgatgcggc ctttcatctc atccgagggc aaggggaccg ccgccaccgt
     6181 cgactccgcc gtctgctcgg cggcgatccg ctgaatcgcg atcgcgacga tcttccgcgc
     6241 ttcgcggtcg gcgttcttct tcgcctgttc cttgatgtcc cggaccagcg cggccgcgtc
     6301 cgagcgcgcc tcgtcttcca gggcgcggac cagctcctgc ttggcgtcgg ccgcggagag
     6361 tcccgccacc cgctcgagcc gggcgcggcc ctcggcttcc agccccgcga gccccttctc
     6421 ccgctccttc agcgcctctt ccagccggcc gatgttgcgc tggcggttct cgatttccgt
     6481 gactttgccg tcgagcgtct gctggcgccg ttccacttcc tgccggcggc ccatcagctc
     6541 ccgctcgagc ccttcgcgcg cccggagcag ttcctctttt acttcgagag cccgcgcctg
     6601 cttggcctcg tcgagctgtc tcgcatgttc gcgctcggag ttgaacgacg tgagggcggc
     6661 gatgcggcgc tcgaaccggc gcccgatgac gtaaaaaacc gcgcccgcgg ccacagcacc
     6721 gccgagcacg gctatgaaga ggcttgccca gcctggcatg agatctatct cctgcaacgg
     6781 tgctccgggg gagcgccgtg actgtttggg ggctatcagc cgtcag
//
LOCUS       DASUBP010000211         7741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_960469, whole genome shotgun sequence.
ACCESSION   DASUBP010000211 DASUBP010000000
VERSION     DASUBP010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7741
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_960469"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            171..488
                     /locus_tag="VFU75_08105"
     CDS             171..488
                     /locus_tag="VFU75_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466839.1"
                     /translation="MPILREWRAEVRRNALDDYVEYVTATGIAHYRETPGNLGAAIAT
                     RELDVHRAEIVVLSWWTDRDVIAAFAGDDIGVARYYPEDDAFLLTKPATVQHYEVKEF
                     GIR"
     gene            604..1209
                     /locus_tag="VFU75_08110"
     CDS             604..1209
                     /locus_tag="VFU75_08110"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin family protein"
                     /protein_id="HEU5466840.1"
                     /translation="MRTHLLTAALIALGTALSAQGAPAAPAPGSQSPNVGDVPPDFTL
                     TGATRFGVLNRPVRLSDLKGETVVLAFFPKARSSGYTIQMKSYREKYAQLFHDGRHVT
                     LIALSNDPADTLAAWAHDEDFNWVFLSDTGAAVGKKYGAYLARWNLDNRYLFIIGPDG
                     KVAFRQTPFLETDPTAYTSLAAAVDKTAPADAHANDGAVKE"
     gene            complement(1213..2082)
                     /locus_tag="VFU75_08115"
     CDS             complement(1213..2082)
                     /locus_tag="VFU75_08115"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU5466841.1"
                     /translation="MRILLVEDNARHAQLLQDMLGEAGAAFAGAPPYDLAHVADLGAA
                     YARIAEGNIDLMMLDLSLPEANGLDALLKVRERFQDLPVIALVARGDGTLAAQAIQSG
                     AQDYLMKGKISGDTLARSIRHAMEVARLRAALRSLSFIDGLTGLYNRRGFVTLAEPYV
                     KRAQRVKGHLLVVSAEVVGFDRIVKLVGYQEGDELLRGVSEIMRRSFRDSDLLGRLEL
                     GEFMALALDAPEEKFPIISGRVAEQVQEFNDRTIRRYRLILQLGFVPFNADLTPTIED
                     LMVQASDARRNQP"
     gene            2269..4080
                     /locus_tag="VFU75_08120"
     CDS             2269..4080
                     /locus_tag="VFU75_08120"
                     /inference="COORDINATES: protein motif:HMM:NF017077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HEU5466842.1"
                     /translation="MPLKRPSYGQPSVVPKLGGLYRRTTAAFVLAVILLLAVGVTTFW
                     GFGRIKAAREDREHTVAVRDRLTRLLVHLQDAETGQRGFLLTGRDPYLKPFEAGGQQA
                     RGDLDTLVQITASDPDISPAVRALTPAIEAKLSELTETVNLRRTNGLSAALPVVQTDR
                     GRALMDSVRQVIRVVARTAADRYTRAGASVGRTTVLVEVVSGTGVLLAIGFLVIAAVV
                     LRHDSSSRQAMETALEEASDEMRDLYERAPCGYHSLDPQGVIVRMNQTECRWLGYQPD
                     ELIGKKTVFDLLAPEDRRSARVRFERLLAGEDIGTGEVRFVKKDGTRFAVLVSSAVAR
                     DASGLPYRVRATMVDQTEFRGARDMVNRLNASLQRHVAELEDVNQELERFSYTVSHDL
                     RAPLRAIAGFANLLKEGHASQLDPEGLRLLGVIHANTERMGRLIDDLLALSRLSRVPL
                     ARAPVDMTSVVAAVVADRKAAGSDGGVKIAVGSLPSVEADPALIKQVWERLIDNAVKF
                     TAGVKGPLVEIGAKTGNGSTVYSVRDNGVGLDMQYAEKVFEVFERLHPQSEYEGTGVG
                     LAVVRRVVHRHGGRVWVESAPGQGATFFFTLDAKGGA"
     gene            4080..4523
                     /locus_tag="VFU75_08125"
     CDS             4080..4523
                     /locus_tag="VFU75_08125"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5466843.1"
                     /translation="MSDADQVEILLVEDSAEDAELTLRALKKHNLANRIHHAKDGVEA
                     LDFLFARGPHADQTTLRMLRVVLLDLKLPRVDGIEVLTRMRADPRTKLVPVVVLTSSR
                     EDPDISRCYALGVNSYIVKPVAFEEFIKVVSSLGLYWLLLNQPPT"
     gene            4534..5994
                     /locus_tag="VFU75_08130"
     CDS             4534..5994
                     /locus_tag="VFU75_08130"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5466844.1"
                     /translation="MTAPPTAAPARIQPFIRGAFTPSAGGGTFPDLNPSNPDDVVAQV
                     PSGAADDVVRATTAAQEAFESWRALPGPARAEHLQKWAEVIGTRREALAQAMSREVGK
                     PIGEARGEVGRGEVILRYYAGEAVRQIGEVIPAQAPEALQLTLREPLGVVGLITPWNF
                     PFAIPLWKAAPALAFGNTVVLKPAEDSSAMASLLAETALQAGIPAGVFNVIPGTGATV
                     GSALIDAPPVRAVSFTGSAAVGASVAAACAARNIRYQTEMGGKNVVIVAVDADLDRAA
                     ALTAAGAMRYAGQKCTATSRAILLRPIADRFLAKLKEQVEALPLGPVDDPKSAVGPLI
                     NAKARDRVSAAVRGAGDFVTGGAPPADPRFHKGYFFSPTVVRDVAPDSRLATEEIFGP
                     VLATFVVDDLDQAIALANSTKYGLSASLFTRDLSSAVRYVRRIDAGLVRVNGDTTGVD
                     PHAPFGGVKGSSSGSREQGPAAREFYTEIKTVQIHP"
     gene            5991..6950
                     /locus_tag="VFU75_08135"
     CDS             5991..6950
                     /locus_tag="VFU75_08135"
                     /inference="COORDINATES: protein motif:HMM:NF017365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline racemase family protein"
                     /protein_id="HEU5466845.1"
                     /translation="MTTIAIPSTMAVVDSHTEGEPTRVVIEGWPTPAGATMAERRDDL
                     KTHADHVRRAVVNEPRGHDAVVGALLTPPVNPGSATGVIFFNNVGYLGMCGHGTIGVV
                     KTLAHLGRLESGEVRIDTPVGTVSATLEADGAVTIENVPAFSHAKDVSIDVRGVGRVT
                     GDIAYGGNWFFLSELPAFPLELRYLDELMKVTKSIMQALKARGITGANKAEIDHVELS
                     GPPSSTNADGRNFVLCPGAQYDRSPCGTGTSAKMAILHERGLLPLEQHWRQESVTGSL
                     FTGWLTRRAEGLIPHIRGRAFITGKTELHFDPADPFQSGLSPS"
     gene            6947..>7741
                     /locus_tag="VFU75_08140"
     CDS             6947..>7741
                     /locus_tag="VFU75_08140"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEU5466846.1"
                     /translation="MTSETASAVVVGAGIVGAAVAEALASDGMRVTIFDSGPAALGAT
                     AVGMGHLVAMDDSPAQLALTSYSLDLWKSLASELPPSAEFDPAGTLWVAEDEMQLEGI
                     EEKVHAYAGRGMPLQWLDGKRVVELEPNLRPGLAGGLLAPRDGILYPPGAALALLERA
                     RARGAQLREHTEVLELGPNQVKTRDGTVRADVIVNAAGTGAARLTPDLPIVPRKGHLV
                     ITDRAPGFCRHQIVEMGYLKSAHQMTAESAAFNVQPRATGQVLIGSS"
BASE COUNT         1295 a         2631 c         2552 g         1263 t
ORIGIN      
        1 actgattgat ccacccctgg aagccgccgc gcagcgtgaa gtgggtcccg aataccttgt
       61 actccccgaa gcacagaaac agcacaccca cggcgattcg caggcagacg agcgcccggg
      121 tgttgtagct atcgttcgaa accgacgtcc gatcttccat cgaggacccc atgcccatct
      181 tgcgtgaatg gcgcgccgaa gtccggcgca acgccctgga tgactacgtg gagtatgtga
      241 cggcgactgg aatcgcgcac taccgggaga cgccggggaa cctgggcgcc gcaatcgcca
      301 cacgggaact ggacgtgcac cgcgcggaga ttgtcgtctt gagctggtgg acggaccgcg
      361 atgttatcgc cgcgttcgcc ggagacgaca tcggggtggc ccgctactat ccggaggatg
      421 atgcgtttct gctcaccaag cccgctacgg tacagcacta cgaggtgaag gagttcggga
      481 tccgctaggc cgttttccac tacgcgaagc tcaccggttt ctgacagcgc gcggaagacg
      541 atgagcgata ggccgagtag aatgcggcgt cgtccatcta ttcctcatca tggagtttgc
      601 tgcatgcgca cccacttgct cacggccgct ctgatcgccc ttggcaccgc actctcggct
      661 cagggcgcgc cggccgcccc cgctcccggc tcccagtccc cgaacgtggg tgacgtgcca
      721 ccggatttca ccctcaccgg cgccacccgc ttcggggtgt tgaaccgccc cgtccgtctt
      781 tcggacctca agggcgagac cgtggtactc gcgttctttc cgaaggcacg gtcttcgggg
      841 tacaccatcc agatgaagtc gtatcgggag aagtacgctc agctcttcca tgacggccgt
      901 cacgtcacgc tcatcgcgct gagcaacgat ccggcggata cgctcgcggc ctgggcgcac
      961 gacgaggatt tcaactgggt tttcttgagc gacacgggcg cggcggtggg gaagaaatac
     1021 ggggcgtacc tggcccggtg gaacctcgac aaccgctact tgttcatcat cggacccgac
     1081 ggcaaggtcg cgttccgcca gacgcccttc ctcgaaaccg acccgaccgc gtacacgtcg
     1141 ctggcggccg ccgtggacaa gaccgcgccc gcggacgcgc acgcgaacga tggagcggtg
     1201 aaggagtaag tcctagggct gattccgtct cgcgtccgac gcctgcacca tcaggtcctc
     1261 gatggtcgga gtcaggtccg cgttgaacgg cacgaatcca agctgaagga tgagccggta
     1321 ccgtctgatg gtccggtcat tgaactcctg cacctgctcg gccacgcgcc cggagatgat
     1381 cgggaacttc tcttcgggcg cgtcgagcgc gagggccatg aactccccca gctccagccg
     1441 gccgaggaga tctgagtcac ggaaggaccg gcgcatgatc tcggacacgc cccggagcag
     1501 ttcgtcacct tcctgatatc ccacgagctt cacgatccgg tcgaacccca cgacttccgc
     1561 cgataccacg agcaggtggc ccttcacccg ttgcgcccgc ttcacgtacg gctccgccag
     1621 cgtcacgaag ccgcggcggt tgtacagccc ggtcaaaccg tcgatgaacg aaagagaccg
     1681 gagggccgcg cggagccgtg cgacctccat ggcgtgacgg atgctccggg ccagcgtgtc
     1741 tcccgagatc ttccctttca tcagatagtc ctgcgctccc gactggatcg cctgagcggc
     1801 gagagtcccg tctccccggg ccacgagcgc gatgaccgga aggtcctgaa agcgctcccg
     1861 gaccttgagg agggcgtcga gcccgttcgc ttccggcagg gacagatcga gcatcatcag
     1921 gtcgatgttg ccctcagcga tccgcgcgta cgcggctccc agatcagcga cgtgggcgag
     1981 atcgtacggt ggcgccccgg cgaatgcagc ccccgcctct cccagcatgt cctgcaggag
     2041 ctgggcatgc cgggcgttgt cctccacgag gagaatccgc accggctcgg tgtcgatgcc
     2101 ggagagggag cgtcgggcga gtttgctgct cgttcgggcc aggagacctc cgcacgggag
     2161 tctggggggt gagccgaccc ggaatcatag aggggacggc accttcaggc gagcagcgcg
     2221 tgcaggagtg ctttgtgcac ttcgcatccg aacgctattc atatagccgt gccgctcaaa
     2281 cgaccttcgt atggtcagcc gtccgtcgtc cccaagctcg ggggactgta ccggcgcacc
     2341 accgccgcct tcgtcctggc cgtcatcctg ctgctcgccg tcggtgtcac cacgttctgg
     2401 ggattcgggc ggatcaaggc ggcgcgcgag gaccgtgagc acacggtggc cgtacgtgac
     2461 cggctgacgc gcctgctcgt gcacctgcaa gacgccgaaa ccggccagcg ggggttcctg
     2521 ctgaccggcc gcgacccgta cctcaagccg ttcgaagccg gaggccaaca ggcccgcgga
     2581 gacttggata ccctcgtcca gatcaccgcg tcggaccccg acatctcgcc ggccgtgcgc
     2641 gccctcacgc ccgcgatcga agccaagctc tccgagctca ccgagaccgt gaacttgcgc
     2701 cgcacgaacg gcctgagcgc cgctctcccg gtggtccaga ccgaccgggg ccgggcgctc
     2761 atggacagcg tgcgccaggt gatccgcgtg gtcgcccgga ccgcggcgga ccgttatacg
     2821 cgtgcgggtg cgagtgtcgg ccgcaccacc gtcctggtcg aggtggtgag cgggaccggc
     2881 gtgctgctgg ccatcggttt tctcgtcatc gcggccgtcg tgctgcgcca cgattcctcg
     2941 tcccgtcaag cgatggaaac cgccctggaa gaagcctccg acgagatgag agatctctac
     3001 gaacgggcgc cctgcggata tcactcgctg gatccgcagg gcgtgatcgt tcgcatgaat
     3061 caaaccgagt gccgctggct cggctaccag cccgacgagc tgatcgggaa gaagaccgtg
     3121 ttcgacctcc tggccccgga agaccgccgg agcgcccggg tccgcttcga acggctcctg
     3181 gcgggcgagg atatcgggac cggtgaggtg cgcttcgtca agaaagacgg cacccgcttc
     3241 gcggtgctgg tgtcgtcggc cgtggcccga gacgcgagtg gcctcccata ccgcgtccgc
     3301 gccaccatgg tggaccagac cgaattccgc ggcgcgcggg acatggtcaa ccgcctcaac
     3361 gcctccctcc aacgtcacgt ggccgagctc gaggatgtga atcaggagct cgagcgcttc
     3421 agctacacag tgtcgcacga tctccgcgcg cctcttcggg ccatcgcggg attcgccaac
     3481 ctgctgaagg aagggcacgc ctctcagctc gaccccgaag gcctccggct cctcggcgtg
     3541 atccacgcca acacggagcg gatgggacgt ctcatcgacg acttgctggc gctctcccgg
     3601 ttgtctcgtg tccccttggc acgcgcaccg gtggacatga cgtcggtcgt cgccgccgtc
     3661 gtcgccgacc ggaaggccgc cgggtcggac ggcggagtga agatcgccgt cggaagtctc
     3721 ccctccgtcg aggccgaccc ggctctgatc aagcaggtgt gggagcggct catcgacaac
     3781 gcggtcaagt tcacggccgg ggtgaagggc cccctggtcg agatcggggc gaaaaccggc
     3841 aacgggagca cggtctacag cgtacgggac aacggcgtgg ggctggacat gcagtatgcg
     3901 gaaaaagtct tcgaagtctt cgagcgtctg catccgcaat cggagtacga gggtacaggt
     3961 gtcgggctgg cggtggtgcg gcgcgtggtc catcgtcacg gcggccgggt ctgggtggaa
     4021 tcggcgccag gccagggagc gaccttcttc tttacactgg acgcgaaagg cggggcgtaa
     4081 tgtctgatgc ggatcaggtc gagatcctgc tcgtcgagga cagcgccgaa gatgcggagc
     4141 tgacgctccg cgccctcaag aagcacaacc tggccaaccg gatccaccac gccaaagacg
     4201 gtgtggaggc cctggacttt ctgttcgccc ggggacccca tgccgaccag accacgctcc
     4261 gcatgctccg ggtcgtgctc ctcgatctca agctcccgcg ggtggacggc atcgaagtcc
     4321 tgacccggat gcgggccgac ccgcgcacca agctggtgcc ggtggtggtc ctgacctcct
     4381 cccgggaaga ccctgatatc tcgcgatgtt acgcgctcgg agtgaacagc tacatcgtga
     4441 agcccgtggc atttgaggag ttcatcaagg tggtctcttc gctcgggctg tactggctcc
     4501 tgctgaacca gccaccgacg tagcttttcg gggatgaccg cgccccccac tgccgcaccc
     4561 gcccgaatcc agccgttcat tcggggagcg ttcaccccgt ccgccggggg cgggacgttt
     4621 cccgacctca atccgtccaa tccggacgac gtcgtcgcgc aggtgccctc cggcgcggcc
     4681 gacgacgtcg tgcgcgcgac gaccgccgcg caagaggctt tcgagtcctg gcgcgcactc
     4741 cccggaccgg cacgcgccga gcatctccag aagtgggcgg aggtgatcgg gacgcggcgc
     4801 gaagccttgg cccaggcgat gagccgcgag gtcgggaagc cgatcggcga agcacgcggg
     4861 gaagtagggc ggggtgaagt gatcctgcgc tactacgcgg gcgaagcggt ccggcagatc
     4921 ggtgaggtca ttccggcaca agcgcccgaa gcgctgcagc tcacccttcg cgagccgctc
     4981 ggcgtggtgg gactcatcac tccctggaac tttcccttcg ccattccgct ctggaaggcc
     5041 gcgcccgcgc tggcctttgg gaacaccgtg gtgctgaagc ccgcggagga ttcgtccgcg
     5101 atggcatcat tgctggcgga gacggcgttg caggcgggga tcccggcggg ggtcttcaat
     5161 gtgatccccg gaaccggcgc gaccgtcggc tcggccctga tcgatgcccc tcccgtgcgc
     5221 gcggtgagct tcacgggctc cgccgccgtc ggtgcctcag tcgccgcggc gtgcgcggcg
     5281 cgaaacatcc ggtatcagac ggagatgggc gggaagaacg tcgtgatcgt cgccgtcgat
     5341 gccgatctcg atcgggcggc ggcgctcacc gccgcgggcg cgatgcggta cgcgggccag
     5401 aagtgcaccg cgaccagccg cgccattctg ctccgcccca tcgccgatcg gtttctcgcc
     5461 aaactgaagg aacaggtgga agcgcttccc ctcggtcccg tggacgatcc gaaatccgcc
     5521 gtcggccccc tgatcaacgc gaaggcccgg gaccgggtga gcgcggcggt gaggggagcg
     5581 ggggatttcg tcaccggggg agcaccgccc gccgacccgc gcttccacaa gggatacttc
     5641 ttctcgccca ccgtcgtgcg ggacgtcgcg cccgactcga gactggccac cgaggagatc
     5701 ttcggccccg tgctggcgac cttcgtcgtc gacgatctcg atcaggccat tgcgctggcc
     5761 aactccacga aatacggtct ctccgcgtcg ctcttcaccc gggatctgtc gagcgccgtc
     5821 cggtatgtcc ggcgaatcga cgccggcctc gtgcgcgtca acggggatac gacgggcgtc
     5881 gatccccacg cccccttcgg tggggtcaaa ggctcgagct cgggatcgcg ggaacagggc
     5941 cctgccgctc gcgagttcta taccgaaatc aagacggtgc aaattcaccc atgaccacca
     6001 tcgcgattcc ctcgaccatg gccgtggtgg attcccacac cgagggggag cccactcgcg
     6061 tcgtcatcga gggctggccc acacccgcgg gcgccaccat ggcggagcga cgcgatgatc
     6121 tgaagacgca cgccgatcac gtcaggcggg ccgtggtcaa cgagccccgc ggtcacgacg
     6181 ccgtcgtggg cgccctgctc acacccccgg tgaatcccgg ttcggcgacc ggcgtgatct
     6241 tcttcaacaa cgtcggctac ctggggatgt gcgggcacgg caccatcggt gtcgtgaaga
     6301 cgctggccca tctggggcgg ctcgagtccg gggaagtgcg gatcgatacg cccgtcggca
     6361 ccgtgagcgc gacgctcgaa gcggacggcg cggtcaccat cgagaatgtg ccggccttca
     6421 gtcacgccaa ggacgtctcg atcgacgtgc gtggcgtggg tcgcgtcacc ggcgacatcg
     6481 cgtacggcgg caactggttc ttcctgagcg agctgcctgc tttcccgctc gagctccggt
     6541 acctggacga gctgatgaag gtgaccaaga gcatcatgca ggccctcaag gcccgtggca
     6601 tcaccggcgc caacaaggcc gaaatcgacc acgtcgagct gtccggcccg ccgtcgtcga
     6661 cgaacgcgga cgggcgcaac ttcgtgctct gtcccggcgc ccagtacgac cgctctccct
     6721 gcggcaccgg cacctccgcc aagatggcga ttctccacga gcgcgggctc cttccgctcg
     6781 agcagcactg gcggcaggag agcgtgaccg ggagcctctt caccggctgg ctcacccggc
     6841 gcgccgaagg actgattccc cacatccggg gccgcgcgtt catcacggga aagaccgagc
     6901 tgcatttcga ccccgccgat ccatttcaga gcggccttag cccctcgtga cgtcggagac
     6961 ggccagcgcc gtcgtcgtcg gcgccgggat cgtgggggcc gcggtggcgg aagcgctcgc
     7021 gagcgatggc atgcgggtga cgatcttcga ctcgggcccc gccgccctcg gcgccacggc
     7081 ggtgggcatg ggacacctcg tggcgatgga cgattctccc gcccagctcg cactcacatc
     7141 gtactctctc gatctctgga aatccctcgc ctccgagctg cctccttccg ccgaatttga
     7201 cccggcagga accctgtggg tggcggaaga tgagatgcag ctggagggga tcgaggaaaa
     7261 ggtccacgcc tacgccggcc gcggcatgcc gctccagtgg ctggatggca agcgcgtggt
     7321 ggagctcgag cccaatctcc gccccgggct tgccgggggc ttgctcgccc cgcgggacgg
     7381 gatcctctac cccccgggcg ccgcccttgc attgttggaa cgcgcccggg cccggggcgc
     7441 gcaacttcgc gaacataccg aggttctgga gctgggtccg aatcaggtga aaacgcggga
     7501 cggcaccgtc cgcgccgacg tcatcgtgaa tgccgccggg acgggcgccg cgcggctcac
     7561 ccccgacctc ccgatcgtgc cgcgcaaggg gcatctggtc attacggatc gcgcgcccgg
     7621 cttctgccgt caccagatcg tcgagatggg ctacctcaag agcgcgcatc agatgacggc
     7681 ggaatcagcg gcgttcaacg tgcagccgcg ggcgaccggc caggtgctca tcggctcgtc
     7741 a
//
LOCUS       DASUBP010000212        28472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1137405, whole genome shotgun sequence.
ACCESSION   DASUBP010000212 DASUBP010000000
VERSION     DASUBP010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 28472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28472
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1137405"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1247)
                     /locus_tag="VFU75_08145"
     CDS             complement(<1..1247)
                     /locus_tag="VFU75_08145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HEU5466847.1"
                     /translation="MVSSRDHLLPPDREAYLAAGQAGRVIVIAPTRAACETIELALGL
                     QIDTLLAREHGDDIERLARSGSGFGIMAGTGTGKTLAIRPIAGAIVGLPLKVGVVNRE
                     REATPETPSWNVVIITTGIARRWFQDGLITRRDTLIVDEIHQTSAELELCLALAKRAR
                     CRCIWLSATVDPEFYARYLESAEVLETTAFDPARAADVRVIPQQPLSFLNERFITRVL
                     RERRGVAVFVPTRAEVEQIASELGARWPRLDTAFYHGGEPIRVIRPFLDGTVKRPFLL
                     AMTAAGQSALNIRGLDTVVIYDARYTNVIERGRNVLTRLYLGANEILQMAGRVHGRVQ
                     GGEVTILSDRRLVFDQLRPTPPEFQLAGDAERVAMTCAAIGVDAGDLDLPVPLDKRSY
                     RAAVTLLTDRGLVENGRLTNYGR"
     gene            complement(1328..1765)
                     /locus_tag="VFU75_08150"
     CDS             complement(1328..1765)
                     /locus_tag="VFU75_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466848.1"
                     /translation="MKRTLMLCLALLAGGAISAKAQTRVIVSFGVGTPGYAGFVSVGQ
                     PWYWYGGERVYYVRRYYEGRPMWYYDPRYVPEDWRGHDRNWFERHRDWDRGRRFDDRD
                     DDHAWHDRGQHRGWYKRDQGDQRDHGDHGDHGKRDHGDHGHHR"
     gene            complement(1919..2986)
                     /locus_tag="VFU75_08155"
     CDS             complement(1919..2986)
                     /locus_tag="VFU75_08155"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HEU5466849.1"
                     /translation="MKIAISTGGGDAPGLNAVIRGVVLAAAHRGWECLGIRHGYDGLM
                     GLDKVVPLGPAEVRGITHLGGTILGTTSRGNPFHWKETRKDGTVVEHDRSKDVVAEAK
                     RLGIDAIVAIGGDGSLGIAMQLYRMGIPVVGVPKTIDNDVGGTVATFGFDTAVQTATE
                     AIDKVHSTAESHDRVMVVEVMGRHAGFIALNSGLAGSADVILIPEIPFDIRKVVEKIR
                     AREAEGRHFSIVVVAEGARPKGGGVSLIDPEHHRLGGIGDKVAHSIEEMTGKETRSMV
                     LGHLQRGGSPTTFDRLLSLRFGAAAVRLIAEKKFGYMVALQPPDIVAVAIEEAISKPR
                     HVPLDSDVIATARDLGISLGD"
     gene            complement(2983..3216)
                     /locus_tag="VFU75_08160"
     CDS             complement(2983..3216)
                     /locus_tag="VFU75_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255349558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(3184..3186),aa:Sec)
                     /transl_table=11
                     /product="SelT/SelW/SelH family (seleno)protein"
                     /protein_id="HEU5466850.1"
                     /translation="MKLSIRYCVVUNYEPRATSLAAEITREFPDAELQLIPGSGGVFE
                     VSRDGVTIFSKKALGRHAQPGEVMALLREAPAA"
     gene            3278..5410
                     /locus_tag="VFU75_08165"
     CDS             3278..5410
                     /locus_tag="VFU75_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEU5466851.1"
                     /translation="MAQEKRGFVLSGVVAVRAVVAGVAIVGVVAAVVAIARGGIPLDA
                     STFLATGVLLAAGAASRRYGIALPGNGFSSYVLGVLILALLSRGWPFAVVVAPAAMLI
                     GDLALRRLPVYAALLNAAHLATGTVLVGLAYELVHGATGASVLTVANLLPLVGILLII
                     PLFINGTFYLELAASQTVAWVDARLTARWEAIVYVVSAGLALGWLGAVESDIPLWAAA
                     TLSLLLAAATIVSVSVIRLGVRADELDLVQRLTGAIAADISLSKSFTRIQDLTRRLVP
                     WEHMGIIRFDERRREMELIADTTMAKSGVTATFRYDADAGLTGEVLRLGRPLVAHDLR
                     PDQVVLAGDETPGAEILIPLHHGGKLMGLWSIRHSDPRMYRESDGELLSLLTPQLALM
                     LALDGSVAPVGTASDKMLQYAETLTATTQEIHASSEEVAASAQRASKSAADAATLVAT
                     ASEGADELRRGAADVAGAGEATRNAGTQMQQTAERVRGATQGAVRRLSDLAATAEESA
                     GEVRRLRDVAGEVEKFSETIGFVANQTNLLALNATIEAARAGQHGRGFAVVADEVHKL
                     AEASAREARNVSKAVQETRRALDRAAQLLERIRADLADVVQTSTSWVQDLDRINEAAV
                     GTAKAGQRVGEVARANADIAGKVVEGLRQGHAGARESTQETEAVAAAAAEQLRAIEDL
                     AQGATQLSSLAENLQRAVRFVRGENARS"
     gene            5331..6875
                     /locus_tag="VFU75_08170"
     CDS             5331..6875
                     /locus_tag="VFU75_08170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466852.1"
                     /translation="MPPSCPRSRRISSEQSASSGERTPGRSWRLGRPFAAALLAVGAC
                     TGATEPGPPVPRIDYVDGAIHPILSAGQSTVIEGFGFELEGVTSSVAFAALGGGTVNA
                     VTDSAGWSDEAIRVVVPVTATSGTLTVHTQSGRTLSTSVFVLPRVPFDTSGFMWQART
                     PFPQSPVGVALAAAEYPASGGGVTTTLYAAGGAEVIGGDSTILPDSGVYVATVASNGS
                     IGSWTRQRDTTFADSNHALPVRLAFASAVVATPYNSRLTGTELIVIGGMDTAGKARAT
                     VYVAAVSQTRVTTAFFPVEPLPAPVAGAIAVARRGRIYVFGGADSAGRPQTGVYIGRV
                     GLDGRIDGWYTVPSLTAPRAYGGGVVLDDRALAFGGIADSADPGGGLDATPSRLVTSD
                     TATLSLVSGFFLGSAWAPGPSLLPQGRSQFSVLPVGNAVLAVGGVCDGGVACPNETLA
                     AALVGDSLGSFHPAGANTISGAGGGVVAGTSGATWRASDGAFHGLVLGGMNLTTRLRV
                     AGVWGF"
     gene            6919..8472
                     /locus_tag="VFU75_08175"
     CDS             6919..8472
                     /locus_tag="VFU75_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HEU5466853.1"
                     /translation="MSRRARTLTGLLLSLAVTQVWAQSPAPATAHAPGAGPTETPTRF
                     EASFFGSFTRYDKAFLLKNTLGGGARIGYLITPNVEVEIEALLEAPQTPAGSPSEVSP
                     IFGGGSLILNLPAGPHLTLYGAAGYTRQDFGGSAPYRFSDGGPHGALGARIFVSPHVA
                     IRAEGREIYIPSSNSTFGSSAFHFVASLGMSFFQAGSAVHLKDSDGDGIADKYDKCPN
                     TPAGAVVDKVGCPIDSDKDGVPDGIDKCPNTPVGAAVDSVGCPKDSDGDGVMDGIDKC
                     PNTPRGAVVDTVGCPVDADKDGVPDGIDKCPNTPPGAVVDSVGCPLDSDHDGVPDGID
                     KCPNTPPGAKVDATGCPQLPALDSDGDGVPDSLDKCPNTPRGMPVDAKGCPILFQTVP
                     AAPGAPPGAAPARPTYVLKGVNFPSGKSVLTPQSYATLDQVAGVLVANPDIRIEIAGY
                     TDNTGSRMINVRLSAARALAVRAYLARKGVSPSRMTARGYGPAEPVAPNTTAAGRAQN
                     RRVELHKLP"
     gene            8560..10929
                     /locus_tag="VFU75_08180"
     CDS             8560..10929
                     /locus_tag="VFU75_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012471.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HEU5466854.1"
                     /translation="MLSLRRSIPGTVILASLALAGPLAGQRPRPKHPAPAPRHAAAPR
                     IPTPRSILGFEPGDDRKLPDWPTLVRYYRALADASDRVDLREIGKSTLGAPFLALVIS
                     SPQNLKRLDHFRAINARLADPRTLANNKDAEQALKDGRTIVMVTSGVHSNEVGGHLSP
                     VQLAYRLATDTSARTRAILDNVILWLVPSLNPDGVTIVSKWYNRTLGTPSEGSQPPEL
                     YHHYVGHDLNRDWYEFTQVETQLVADSLYGVWHPQIVMDLHQQGQNGSRLFLPPYLDP
                     IEPNVDPLLVDGDNALGTSIAWTLGGEGRTGVAINSLYDAWTPSRAFAHYHGAVRILT
                     EAASAELATPVEVPFDQLSAAGRGFNPRERSWNFPHPWPGGRWTLRDIVSYQTDAAYA
                     LLEQASTDRTRWLSDFLTVGWRGVRGWRGWPWAYAIPRQQDSIALATLFSVFHHGGVE
                     VRSADQAFSAGGQRYAAGTYIVTLRQPYAAFAKTLLEPQAYPDRRLYPNGPPERPYDV
                     TANTLPLLLGLTATPLTEEPARLQLSAPIAAPHATPGYPGFGSGEVPKVAIYRSYAAS
                     MDEGWTRWIFDTWKVPYVSIVDSTMRAGGLKAKFDVIILPDESPHDLLEGLPSPRYPA
                     AYAGGLGSSGVDALRQFVQDGGTVVALNESSRFLIQTLLLPVRNVLEGVGDDEFYAPG
                     SIFRLELDTTAAEAHGMPPRSIAWFEGGPAFDVLDSAQVRVIGRYPTDPNSLLLSGWV
                     LHPERIAGRAAMVEVKVGQGKAVLFGFRPQYRGQSLATYPLLFNSLEMK"
     gene            10981..12204
                     /locus_tag="VFU75_08185"
     CDS             10981..12204
                     /locus_tag="VFU75_08185"
                     /inference="COORDINATES: protein motif:HMM:NF024598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HEU5466855.1"
                     /translation="MRLRRDRRLLLVLAALAFPGRLFAQGRADSTRTLIPVRADQLPI
                     FLHPGTIHFAHPDQPMFVNARNVKALYVNAWAFASPKLWGLARLADSTEINALVVDVK
                     DDTGCMLYPSQVPTAIEIGASACARTKNVEARLDTLRAHHIYTIARIVVAKDPRLAER
                     KPEWSVKDKRGGLWRDRINIAWVDAYNDSVWIYAAQLAREAVEKGFNEVQFDYVRFPD
                     EPRAKLAEAIFPAKKPGDTERVAVRRHVQLLVDRVRSAGVPATFDIFALTASTTEGDL
                     GIGQVWEDFASVADVVLPMVYPSHYYAGAFGFAHPNGEPYRIVHDALRDAQEKSRDIP
                     NAATIRPYLQAFTLGRRKPRYTPVEIREEIRAAEELGITSWVLWNPRSVYQRDALRPF
                     RTTSHPSETEAGVGE"
     gene            12233..12970
                     /locus_tag="VFU75_08190"
     CDS             12233..12970
                     /locus_tag="VFU75_08190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466856.1"
                     /translation="MRKVGVGMVLAAWFAAACSSSTTSVSADSIDGAELAAVRAALGK
                     ALASDSFYAELSTFVFPFIDQATKEGEGPGDTTRLVGILLDINATKGDTPIVARFSAL
                     LDWRHYRPTSQTVDSVTFLLGAGITPPISDSLATSFSPDSVGTGVGFVVAQAQDSAVQ
                     TWLTRAGAFHLTGASFGSAQSTGLGGLTLAVSHGTMNGDFHLTAKLEPDSSTTVTGTR
                     SFSGGIHALKIAITGSLSAPPAPAPTR"
     gene            complement(12924..14951)
                     /locus_tag="VFU75_08195"
     CDS             complement(12924..14951)
                     /locus_tag="VFU75_08195"
                     /inference="COORDINATES: protein motif:HMM:NF012550.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HEU5466857.1"
                     /translation="MLEALKSRPVNKLPDRVRFPGRILFLTEDAGLVREQLAGKDLPW
                     PPKVPLRDNISTDEITPAYICYYYDETLGEFPYLGLKCGDQFPITRGSVKQGGFVASV
                     SGKRRGKGSSREQSPYAEMCAGIKLVVAENIERIYRENCQNLGVLTTTDFSIIERVRR
                     GEEIPLSAFTEGEGEITRGIIEYGGLFNYNVARLQGKVVLSPPPTPRRPMTLGEKVLA
                     RHWVVDPSKGKVGVPSVKPGDEGFVVTDIRFSHEYVTPMAAIFFEQLVGQDEKVLDPG
                     SVLLFRDHLTFLGEAMTPERIKEGLLDVANQLEKKQRAFAEKQGIKLYGELRLGHHGS
                     EAICHSKILESHALPGQVIIGSDSHTPHAGAVGCVAFGVGTTAIFNSWITKDVRLTVP
                     ATVKVVVKGAKPANVTAKDYMLEILRHPYIKSGQAIGKLVEYCGGAVEALSVDERATM
                     TNMTAEVGGFTGYVAPDEKTVAYLMDYRKMTRAEAEKACEGIASDPNAEYCEVIEIDA
                     SRLKPMVALPGDPGNGAYIAELGAPVKVEIAYAGSCTAGKKEDMDMYARVLKEAVDRG
                     QKVAPGVKFFIQSGSQDVKRYCEEQGYLDIFRQVGATFLEPSCGACINAGPGVSRTKD
                     EVTISAINRNFPGRSGPGQLYLANPYTVAASAIAGVITEWAPAPEARSKSR"
     gene            15003..16640
                     /locus_tag="VFU75_08200"
     CDS             15003..16640
                     /locus_tag="VFU75_08200"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5466858.1"
                     /translation="MRPLYTLLLIFGASSGPALAQKPATPATPAGPPPPSIPAPNERA
                     MDAHLRFLASDLLEGRAPATRGGHLATDYIAAQFEALGLEPAGPNGSYFQPVSLAGTL
                     TQQPSFVWGKGADTRSLKPGDEFVEWAMKPESRITVDGDVVFVGYGIKAPEYRWDDYK
                     GADLHGKVLLMLVNDPGIQDSTLFKGRVLTYYGRWTYKLEEAARQGAVGVVLVHTTPS
                     ATYGWDVVRGSWSVEQFQLEGDAGPSLDFASWATHDAVQSALASVGLNLDSLTRLAAT
                     RGFVPVSTGLHAAIDLASAVRHVQSENVVARLRGSDPVLSSQVVVISAHWDHKGIGPA
                     VGDDSIYNGAEDNASGVAAMLATAEALSKMPKRPRRSVLFVATTAEESGLLGSAGYVA
                     HPLFPLAQTAADINMDEVNVHGATRDIAALGTDQSSLGKMFADAAQAESLKVANRPDI
                     GGGFFRSDHFPFAKAGVPALSIQAGEDFIGKPAGWGRQEEQDFEQHRYHQPSDEWTPL
                     FRYDGMSQEVRVVMRLTLAIADATDLPQWLAGADFKR"
     gene            16883..17911
                     /locus_tag="VFU75_08205"
     CDS             16883..17911
                     /locus_tag="VFU75_08205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466859.1"
                     /translation="MAEAAPEGARDPERLAELAKQALEYKPAQAIARKQKDTGLWGAN
                     LLAPGPNKVFGWTETGTVTQYRHLLELGWPADQRVFRIADRFLFRLLSRDPDPTLLVE
                     FQRPAKTDPGLGLWARNLGREAAAAALARASRGEDPRLRGAAHNILNGISTYLRSPLL
                     EKPFKKAQGKTVVEPEAVPPSYFAMEMLAFLPALQRERSGFIERLGAYFSTPAPKRAF
                     FQLAGKKVLPPTFIIMGDPLRADAHGHVPDVPFAVYWLEILARLGLVRQLPSATKVLA
                     RLYSECDDQGIWSPKALRTMPKSDSPVLSHYFPLEGPGKSPAQKQTDITFRLALIARL
                     LGLPIEVV"
     gene            17955..18605
                     /locus_tag="VFU75_08210"
     CDS             17955..18605
                     /locus_tag="VFU75_08210"
                     /inference="COORDINATES: protein motif:HMM:NF014080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5466860.1"
                     /translation="MTSIDLTPFGFTPTESLVYASLLRLGPSTGYAVARACRLARANA
                     YAALEGLVSRGAATSAGRPAQFRPADPSSLVARVAASQGQALERLSRALEGAAAGSEP
                     TIHSAEGLRGTVNAVQQLVARAERSVRGTVAAELWRPSLPAWRRAATRASLDVLIAGE
                     AEDPESLARGTAPASSATILLIDEVRVFSASGAGDLMTAMWTAHPLLVQVGSAAMR"
     gene            18602..19579
                     /locus_tag="VFU75_08215"
     CDS             18602..19579
                     /locus_tag="VFU75_08215"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5466861.1"
                     /translation="MKTPPLLGFLALGAVGCRAAPIPESQWYPAGTNLRAKYVTVQGT
                     RIRYVESGAGPTVVLLHGLGASIYTWRDVIGPVAAAGFHVVAFDNRGFGGSDRPTSGY
                     SAGDYASLAVAFLDSLHLSDVVLVGHSMGGEIAALATLKDPSNVRGLVLIDAAGLGTH
                     LPLLTRLARAPLVGHVIAGLRGRSGVERALRSTFGVPSRVTQDEVDQYYAPVAMPDFG
                     RTLAGVAREFDFSALKGRLDSIAKPTLVLWGSRDIWIPPGIGERMTASLQRAAFVLVP
                     GVGHDLPDEDPAALLRSLIPFLQQGIPQPPPNVAILGIDINQQVDKLEQ"
     gene            19576..20169
                     /locus_tag="VFU75_08220"
     CDS             19576..20169
                     /locus_tag="VFU75_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5466862.1"
                     /translation="MKHKLSNSRALRIIDSAQREFAREGFEGARVDRIAKSAGVNKQL
                     LFYYYQSKQGLFRAVLGRAAADLEQSLAGLTASGGRPLDRLERSLDLLYNFFAAHPEL
                     VALLTRSGRVDIAPFAPVVKRLVVLLAEGQGLGQVRDDVDPHLTAAQALVLMVGFLNL
                     ESVVAASAPSLAMDEPALRQRWREGATRLLVAGVAAR"
     gene            complement(20181..22604)
                     /gene="lon"
                     /locus_tag="VFU75_08225"
     CDS             complement(20181..22604)
                     /gene="lon"
                     /locus_tag="VFU75_08225"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HEU5466863.1"
                     /translation="MYERLTLPVLPLREMVLFPGVSQPIGAGRPATLRAIEAALRSQS
                     HLIFAVSQRENVDQVTPGVLYTTGTIAKISQIQRGIGGVQLMLHGEVRGTALHYQEAS
                     GHLEAVVREATDLPPLDEQNQAFVALYREARERAGDLGRKSGLPEEVVKQVLDDVNEP
                     GRFADLVAGYLEVKPSEKQSLLETLGVEDRLRRVLVHVQRQIGMLDAQEDIKSQVQEE
                     LGERQREMYLREQLKAIHKELGDEDEAGDVDDLRKRIAALDLPEAARKEVDRELLRLD
                     RANKDSMEGQVIRTFLETVAELPWNKRSEESLDLERAAKVLEEDHFGLQEVKDQVLEF
                     LAVRKLRLEKPDTEAKGNDDDKQAKGPILLFIGPPGVGKTSIAKSIARAMGRPYVRIS
                     LGGARDEADIRGHRRTYVGAMPGRIIQGMKQAGAKNPVFLLDEVDKLGVSFQGDPASA
                     LLEVLDPAQNDSFTDHYLGVPFDLSEVLFIATANFPQNIPGPLMDRMETVTFAGYTER
                     EKQEIARRYLIPRQIQENGLTAEQLTITDDALQVVISSYTREAGVRQLEREIGKVGRK
                     AARKIAGKDAVVVNVTAADVPALIGRPKVHPERKAGADDVGVATGMYYTPVGGDIMFV
                     EASVMRGKGELTLTGQLGDVMKESARAAWTYAKSHADQLGIPEGRLQDSDVHVHVPAG
                     AIPKDGPSAGVTMATALVSALSGRPARHDLAMTGEVTLRGKVLPIGGVKEKVLGAVRA
                     GIQTIILPQENGRDLEDLPAEVRQMLDVHLVSDLGQVLSLALRGARFEAGHLVFEGDA
                     PKEPRMVGR"
     gene            22893..24743
                     /locus_tag="VFU75_08230"
     CDS             22893..24743
                     /locus_tag="VFU75_08230"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU5466864.1"
                     /translation="MSENDNSLMGRLSRALGSAYAVDSEIGRGGMGVVYSARDERLKR
                     RVAIKVLPPELAFREEIRLRFLREAETAARLSHPHIVPIFSVGEGPDGLVYFIMAFVD
                     GEPLSGKLQRRGTLPPEEARRIMLETVDALGAAHAVGIVHRDVKPDNILLEGSRGRVM
                     VTDFGIAKALTSSTGPGTLTGTGVAIGTPHYMSPEQALGEREIDGRSDLYSLGVVGYQ
                     MLVGELPFSAPTVPGLLMKQITEEAPDITSKRSDCPSELSACVMRCLDKDPEKRWPTA
                     DALRRALESRSTPAFRPSPRVVTSRGSSGLNRPAAPQGRAPLPVPRSPDVGRGRSLRR
                     QQRDRDRNQPQKTPGGEAAMVKATRANFVSWASVSCGLLFLNLVTGGGVGWSIWPIGL
                     WGAFGVLPRFWRLWEAGYSWRDVFMRPAAPDAAESRLSPAVGTPLIPASTDEYGNEAA
                     TIDQVRRDRQAIFGVVQRLPQSDKERLPEVTQTVDALMARAENLAKMLHAMSADVDGT
                     SVPRLDERIAQVKAQPEGSERDRQLGLLERQRQALADLVGRRDGVREQLESCVLAMQN
                     VRYDLLRLKSAGIGAVLGDLTQATQQARALSRDVDHAIAAAGEVKDLMRS"
     gene            complement(24740..26872)
                     /locus_tag="VFU75_08235"
     CDS             complement(24740..26872)
                     /locus_tag="VFU75_08235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase E1 component subunit alpha/beta"
                     /protein_id="HEU5466865.1"
                     /translation="MATKTPPQSRAHTKSAKSQLSKAELLDMYRLMLLSRRLDDKEIQ
                     LKRQNKIFFQISGAGHEAILVAAGKAMKPSHDWFYPYYRDRALCLTLGMTPTEMLLSA
                     VGAAEDPNSGGRQMPSHWGHQALNIVSQSSPTGTQFIQAVGCAESWYRASKIADADVS
                     VKKDEVVYCSSGDGTTSEGEYWEALNTACNLKLPVVFLVEDNGYAISVPVEVQTAGGS
                     VSKLVTGFPDLFIQEVDGCDPLASYEAMRRAVEYCRERKGPALVHAHVTRPYSHSLSD
                     DEILYRPTKEREEEAKRDCVIVFPKKLVADGIATQAEIEAIQKQVDDDVQVAADMALV
                     SPQPKTDTVYNYVYSPDVDPTSEQFDTEDDPQFKGEPTTVVDLLNACMKDEMARDPRI
                     LVFGEDVADASREAVLKDVKGKGGVFKVTWGLQRQYGGDRVFNSPLAEANILGRAIGL
                     STRGWKPVVEIQFFDYIWPAYHQIRNELATMRWRSNNAFKCPAVVRVTYGGYLKGGSI
                     YHSQTGAVVFTSVPGLRVICPSTALDANGLLRTAIRCDDPVLFLEHKHLYRQTYNKAP
                     NPGPNFMIPFGKARVAREGTDLTVITYGAVVQRALVAAKMLEDADGTRVEVIDLRSLS
                     PVDWETIEQSVRKTNKVLVAYEDSLSWGYGAEIAAKVADVCFPWLDAPVKRVASTDTF
                     VAYAPELEDVILPQSEGLAAAMRELKAF"
     gene            complement(26889..>28472)
                     /locus_tag="VFU75_08240"
     CDS             complement(26889..>28472)
                     /locus_tag="VFU75_08240"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU5466866.1"
                     /translation="RSPMPRNASTRCARALLVALLAAAPVTGGAAQQRTSYEELQTFS
                     QVLTYIFHSYPDSVSYSILVKSAIDGVLRDLDPHSLFFSRLDYERFDSLQRGDLATTG
                     ASLDDVEGEPTVLGIYPKSPAEDGGVRPGDRVLAINDTVIAGLEIETVRLRMAGAPGT
                     KVRVRFARGPKLEPDTFTVTLKRKAFEERDVWSVRMVDSVTGFLRLDEFGEKSADQVH
                     DAIKSLKKQGAKQIILDLRNNPGGIVDQAVDLVSEFVPKGTIVFSTRGRRADVDTTYR
                     TRKDGDFRDLPLIVLVNQLSASAAEATAASLQDHDRALIIGRRTFGKALMQSNFILSS
                     GEVLHMTIGHVISPSGRYIQRRYAGLAVEQYYDLAGKAGAANDTDKVYHTDAGRAVRG
                     GGGVLPDIVDSGGVQPAAWWAAAQDSGWITAVVDSVALTQPATPAGMAAFEASLPRWG
                     NTLMPGLLKRIRDRLHVEAMVDSTTQWRLGYEVGRRVVENRWGVDAMYDLDLHHDRDV
                     RLALGAFPRLSDLLKAKPK"
BASE COUNT         4521 a         9472 c         9560 g         4919 t
ORIGIN      
        1 tggcgcccgt agttggtgag gcgtccgttt tccaccaggc cccggtcggt gagcagcgtg
       61 accgccgccc gatacgaccg cttgtcgagg ggcacgggca gatcgagatc cccggcgtcc
      121 acgccgatgg cggcgcaggt catcgcgacc cgctccgcgt ctccggcgag ctggaactcg
      181 ggcggtgtcg ggcgcagctg gtcgaacacc agccgccggt cactcaggat ggtgacctct
      241 cccccctgca cgcggccatg cacgcgcccc gccatctgca agatttcgtt cgcaccgagg
      301 tagagccggg tgaggacgtt ccggccccgt tcgatgacgt tcgtgtagcg cgcgtcatag
      361 atgacgacgg tgtcgagccc gcggatgttg agggccgatt gccccgccgc cgtcatcgcg
      421 agcagaaagg gccgcttgac ggtgccgtcg agaaagggcc tgatcacgcg gatcggttcg
      481 ccgccgtggt agaacgccgt gtcgagcctc ggccaccgcg caccgagctc cgatgcgatc
      541 tgctccactt ccgcccgcgt cggcacgaag accgcgaccc cccgccgctc gcgcaggacg
      601 cgagtgatga agcgttcgtt caggaacgag agcggctgct ggggaatgac ccggacgtcg
      661 gcggcgcgcg ccggatcgaa cgccgtcgtt tccagcactt cggcggactc gaggtagcgg
      721 gcgtagaact cggggtcgac ggtggcggag agccagatgc agcggcacct ggcccgcttg
      781 gccaacgcga gacacagctc cagctcggcc gaggtctgat ggatctcgtc gacgatgagg
      841 gtgtcgcgcc tggtgatgag tccgtcctgg aaccaccgcc gcgcgatccc ggtggtgatg
      901 atgaccacgt tccaggaggg cgtttcgggc gtcgcctccc gctcgcggtt cacgactccg
      961 accttgaggg gaagccctac gatggcgccg gcgatcggcc tgatcgccaa tgtcttgccc
     1021 gtccccgtcc cggccatgat gccgaagccc gatcccgaac gcgccagccg ctcgatgtcg
     1081 tcgccatgct cccgtgcgag cagggtgtcg atctggagac cgagggcgag ctcgatcgtt
     1141 tcgcacgcgg cgcgggtggg ggcgatgacg atgacgcggc ccgcctggcc ggcggcgaga
     1201 taggcctcac gatcgggagg gaggaggtga tcgcgggagg ataccacgct tcaatattag
     1261 tagaaggaaa aagagaacgg cggccggggt tgtccccgac cgccgccatt gcttcagcga
     1321 ctaccgccta tcgatggtga ccgtggtcgc cgtgatccct cttgccgtga tccccgtgat
     1381 ccccatgatc gcgttggtcg ccttgatcac gcttgtacca gccgcggtgc tgcccgcggt
     1441 cgtgccatgc atggtcgtcg tcgcgatcgt cgaacctccg gccgcggtcc cagtcacggt
     1501 gtcgctcgaa ccagttgcgg tcatgaccgc gccaatcctc gggtacatag cgcggatcgt
     1561 agtaccacat cggtcggccc tcgtagtagc gccggacgta gtagacgcgt tcaccaccgt
     1621 accagtacca aggctgacca accgagacga acccggcgta accaggcgtt ccgacgccga
     1681 aggacacgat cacgcgggtt tgcgccttcg ccgagatggc cccaccagcg agaagcgcca
     1741 ggcacaacat cagggtccgt ttcatatgac ctcctgtggt caccccctac cagtggaagt
     1801 cccgtgccaa gcgggctttt cgaggcgaac tcgtgctgag aatcaggagg ttaggaggtg
     1861 tgaggcggtt cgacggtgaa acggaggaaa atgtcctcag gaggggacga cagggagcct
     1921 aatcccccag ggaaatgccc agatcgcgag cggtggcaat gacgtccgag tcaagcggca
     1981 catggcgcgg cttcgagatg gcctcctcga tggccaccgc cacgatgtca ggcggctgaa
     2041 gcgcgaccat gtacccgaat ttcttctcgg cgatcaggcg gaccgccgcg gcgccgaatc
     2101 gcagggagag gagccggtcg aaggtcgtgg gggagccgcc gcgctgcaga tgaccgagca
     2161 ccatcgagcg ggtttccttc ccggtcatct cttcgatcga atgcgccacc ttgtcgccga
     2221 tgccgccaag ccggtggtgc tccgggtcga tgagggagac cccaccccct ttggggcgcg
     2281 cgccttccgc cacgacgacg atgctgaagt ggcgcccctc ggcttcgcga gcccggatct
     2341 tctcgacgac cttcctgata tcgaagggga tctcagggat caggatgacg tcggcggacc
     2401 cggcaagccc ggagttgagc gcgatgaatc cggcgtggcg gcccatcacc tcgaccacca
     2461 tcacccggtc atggctctcg gccgtggagt gcactttgtc gatggcttcg gtcgcggtct
     2521 ggacggcagt atcgaatccg aaggtcgcca cggtcccgcc gacgtcgttg tcgatggtct
     2581 tcgggacgcc gacgacggga atccccatcc ggtagagctg catggcgatg ccgagcgagc
     2641 cgtccccgcc gatcgccacg atggcgtcga tgcccagccg cttggcctcg gctacgacgt
     2701 ccttcgaccg gtcatgctcg acgacggtcc cgtccttcct tgtttctttc cagtggaacg
     2761 ggttgccgcg gctggtggtg cccaggatgg tgccaccgag gtgggtgatc ccgcggacct
     2821 ccgcggggcc cagggggacg accttgtcga ggcccatgag cccgtcgtag ccgtggcgta
     2881 tgccgaggca ttcccagcca cggtgagcgg cggcgagtac gacaccgcgg atgacggcgt
     2941 tgaggcccgg ggcgtcaccg ccgccggtgg agatggcgat cttcacgcgg cgggtgcttc
     3001 gcgcagcaat gccatgacct cccctggctg agcatggcgc ccgagggcct tcttggagaa
     3061 gatggtcact ccgtcacgag acacttcgaa cacgcccccg gatccgggaa tgagctggag
     3121 ctcggcatcg ggaaactcgc gggtgatctc ggccgccaaa ctggtggccc gaggctcgta
     3181 gtttcagacg acgcagtagc gaatggaaag cttcacccga gtagcatagc cacgcttgac
     3241 acgccgcgcc acacgcccga gcttgcgcgg accccgcatg gctcaagaga aacgcggctt
     3301 cgtcctttca ggagtggtcg ccgtccgcgc cgtggtcgcg ggcgttgcga tcgtcggcgt
     3361 cgtcgccgcc gtcgtggcca tcgccagggg cgggattccc ctcgacgcct ccaccttcct
     3421 cgccaccggt gtgttgctcg cggccggcgc cgcgtcccgg cggtacggca tcgcgctccc
     3481 cggcaacggc ttctcctctt atgtgctggg cgtcctcatc ctggcgctct tgagccgtgg
     3541 atggcccttc gccgtggtcg tggcgccggc cgcgatgctg atcggcgatc tggctttgcg
     3601 acgacttccg gtttacgccg cgctgctcaa cgccgcgcac ctggcgacgg gcacggtcct
     3661 cgtgggactg gcctacgagc tggtgcatgg ggccacgggc gcgagtgtcc tgacggtggc
     3721 gaacctgctc ccgctggtcg ggatcctgct gatcatcccg ctcttcatca acggtacgtt
     3781 ctatctcgag ctcgccgcgt cccagacggt agcctgggtg gatgcgcgcc tcacggcccg
     3841 gtgggaagcc atcgtctacg tcgtatccgc cgggctcgcg ctcggatggc tgggcgcggt
     3901 cgaatcagac atcccgctgt gggccgcggc gacgctgagc ctcctcctcg ccgccgcgac
     3961 gatcgtcagc gtatccgtga tccggctggg ggtccgtgcc gacgagctcg acctggtgca
     4021 gcggctcacc ggggccatcg cggccgacat cagtctgtcg aagagcttca cgcgcattca
     4081 ggatctcacc cgccgactgg tgccgtggga gcacatgggc atcatccggt tcgacgagcg
     4141 ccggcgggag atggagctca tcgcggacac cacgatggcg aagagcggcg tcacggcgac
     4201 tttccgttat gacgccgacg ccgggttgac gggagaggtt ctccggctcg gccggccgtt
     4261 ggtggcgcac gatctccggc ccgaccaggt ggtgctggcg ggcgacgaga ccccgggtgc
     4321 ggagatcctc attccgctcc accacggcgg gaaactgatg ggcctctgga gcatccggca
     4381 ttcggacccc cggatgtacc gcgagtcgga cggcgagctc ctgagtctcc tcacgcctca
     4441 gctggcgctg atgctggcgc tcgacggctc cgtggctccc gtaggtaccg cctcggacaa
     4501 gatgctgcag tacgccgaga cgctcacggc cacgacgcaa gagatccatg cttcgagtga
     4561 ggaggtcgcg gcgtcggccc agcgggccag caagagcgcg gctgacgccg cgacactcgt
     4621 cgccacggcc tcggaaggag ccgacgagct ccgacgcggc gccgccgacg tcgcgggcgc
     4681 cggcgaagcc acccggaacg ccgggaccca gatgcaacag accgcggagc gcgttcgtgg
     4741 ggcgacgcag ggagccgttc gccgcctgtc cgacctcgcg gccactgccg aagaaagtgc
     4801 cggggaggtc cggcggctcc gggatgtcgc cggcgaagtg gagaagttct cggagaccat
     4861 cggcttcgtc gcgaaccaga ccaatctgct ggcactgaac gccaccatcg aagcggcccg
     4921 ggccgggcag catggccggg ggttcgcggt ggtggccgac gaggtccaca agctcgcgga
     4981 agcgagcgcg cgggaagccc ggaacgtgtc gaaggccgtg caggaaaccc gccgcgccct
     5041 ggaccgtgcc gcgcaactgc tcgagcggat ccgggccgac ctggccgacg tggtgcaaac
     5101 gtccaccagt tgggtacaag acctcgaccg gatcaacgaa gcggccgtcg gcaccgcgaa
     5161 ggccggccag cgcgtgggtg aggtcgcccg cgccaatgcg gacatcgcgg ggaaggtcgt
     5221 cgaaggcctg cggcagggac acgccggtgc acgggaatcc acacaggaaa cggaggcggt
     5281 cgccgccgcg gcggccgaac agctcagagc gattgaagac ctcgcccagg gtgccaccca
     5341 gctgtcctcg ctcgcggaga atctccagcg agcagtccgc ttcgtccggg gagagaacgc
     5401 ccggtcgtag ttggcgcctg gggcgtccgt tcgcggccgc cctgctcgcg gtcggagcct
     5461 gcacgggcgc caccgaaccg gggccgccgg tcccccgcat cgactacgtc gacggcgcca
     5521 tccaccccat tctctccgcg ggccagtcca ccgtcatcga aggcttcgga ttcgagctcg
     5581 agggcgtcac gtcgagcgtc gcattcgccg cgctcggtgg gggcacggtg aacgcggtga
     5641 ccgacagtgc cggctggagc gatgaagcga ttcgcgtcgt cgtccccgtc accgccacgt
     5701 ccggcacgct tacggtacac acccagagcg gcaggaccct cagcaccagc gtcttcgtcc
     5761 tgccccgcgt gcccttcgac accagtggat tcatgtggca ggcccgcacg ccgttccccc
     5821 aatcaccggt gggagtcgcg ctcgcggccg ccgagtaccc ggcgtcgggc ggcggggtga
     5881 cgaccaccct ctacgccgcc ggcggcgccg aggtcatcgg cggggactcg acgattctgc
     5941 ctgattcggg cgtgtacgtc gcgacggtgg cgagcaacgg atcgatcggc tcgtggaccc
     6001 gccagcgcga cacgacgttc gcggactcga accacgcgct gcccgtccgt ctcgccttcg
     6061 cgagcgcggt cgtggccacg ccttacaact cccgcctgac cggcaccgag ctgatcgtga
     6121 tcggcggcat ggacacggcg gggaaggcac gggcgacggt gtacgtcgcc gcggtttccc
     6181 agacgcgcgt cacgacggcg ttctttccgg tggagccgct cccggctccc gtcgcgggcg
     6241 ccatcgcggt ggcgcggcgg gggcggatct acgtgtttgg cggcgctgac tccgcgggcc
     6301 ggccgcagac cggcgtctac atcggccgcg tgggcctgga tggccggatt gacggctggt
     6361 acacggtacc gtcgctcacg gccccccggg cgtacggtgg cggcgtcgtg ctcgacgatc
     6421 gcgctttggc gttcggcggc atcgccgaca gcgcggatcc cggagggggg ctggacgcga
     6481 cgcccagcag attggtgacg agcgacaccg ccacactcag tctggtctcg ggattctttc
     6541 taggctcggc ctgggccccg ggacccagtc tcctcccgca ggggaggtcg cagttctccg
     6601 tcctcccggt cggcaacgcc gtcctggccg tgggtggcgt gtgtgacggc ggcgtggcct
     6661 gcccgaatga aacgctggcg gcggccctgg tgggggacag tctcgggagc ttccacccgg
     6721 ccggcgccaa tacgatttcg ggagccggcg gaggggtcgt cgccggcact tccggggcga
     6781 cttggcgggc cagcgacggg gcctttcacg gcctggtcct cggcggcatg aacctgacga
     6841 cccgactccg ggtcgctgga gtttggggat tctgactgcg cttattagcg tagagcgcga
     6901 atgacccgtg gagggtccat gagcaggcga gctcggacgc tgaccggttt gttgctgtcg
     6961 ctggccgtca cgcaggtgtg ggcgcagagt cccgccccgg caacggcgca cgcccctggt
     7021 gcgggcccaa ccgaaacacc cacgcgattc gaagcgagct tcttcggctc cttcacgcga
     7081 tacgacaagg cctttctcct caagaacacg ctcggtggag gcgcccgcat cgggtatctc
     7141 atcacgccga acgtcgaggt cgagatcgag gcgctgctcg aggctccgca gacaccggcg
     7201 ggatctccgt cggaggtctc gccgatcttc ggcggcggtt cgctgatcct caatcttccc
     7261 gccggtcccc atctgacgct gtatggtgcc gccggctata cgcgtcagga cttcggcggc
     7321 tcggctccct accgcttttc tgatggcgga ccgcatgggg cgctgggagc gaggatcttc
     7381 gtgagtccgc acgtcgccat ccgcgccgaa gggcgtgaga tctacatacc gtcctcgaac
     7441 tcgacgttcg gaagctcggc cttccatttc gtggcgtcgc tcgggatgtc gttcttccag
     7501 gcgggaagcg cggtccatct caaggactcg gatggagacg ggatcgcgga caagtacgac
     7561 aaatgcccca acacccccgc cggcgccgta gtggacaagg tcgggtgtcc catcgactcc
     7621 gacaaggacg gcgtgcccga cggcatcgac aagtgtccca acacgccggt cggcgcggcg
     7681 gtggattccg tcggctgtcc gaaggactct gacggggacg gcgtgatgga cggcatcgac
     7741 aagtgcccca atacgccacg cggtgcggtc gtcgacacgg tgggctgtcc ggtcgatgcg
     7801 gacaaggacg gcgtgcccga cggcatcgac aagtgtccga acacaccgcc gggcgccgtc
     7861 gtggactcgg tgggttgccc cctggattcg gatcatgacg gcgtgcccga cggcatcgac
     7921 aagtgcccga acacgccgcc gggagccaaa gtggacgcga ccgggtgtcc ccagctcccc
     7981 gctctggact cggatgggga tggcgtgccc gattcgctcg acaagtgtcc caacacacca
     8041 cgcggcatgc cggtggacgc gaagggttgc ccgatcctct tccagaccgt gcccgccgcg
     8101 ccgggagctc ctccaggagc cgcgccggct cggccgacgt acgtattgaa gggcgtcaac
     8161 ttcccgtccg ggaagtcggt gctgacgccg caatcgtacg cgactctcga tcaggtggcg
     8221 ggggtgctgg tcgcgaatcc ggacatcagg atcgagatcg cgggctacac cgacaacacc
     8281 gggtcgcgga tgatcaacgt ccgcctttcg gcggcccgag ccctcgcggt gcgggcgtac
     8341 ctggcgcgga agggcgtgtc cccgtcgcga atgaccgcac gcgggtacgg tcctgccgaa
     8401 ccggtggcac cgaatacgac cgcggcgggc cgggcgcaga accgccgcgt ggaactccac
     8461 aagcttccct gacctttcga gaggcggggc gccggtcgct ttcggcgccc cgccccgata
     8521 tttttcgccc ccactagcaa cccacgaagc gggtcaccga tgctctcgct ccggcgttcg
     8581 ataccgggga ccgtcattct cgcgagtctc gcactggccg gccctctggc cggccagcgg
     8641 cctcgcccca agcatccggc tcccgctccc aggcacgccg ccgcgcccag aatcccgact
     8701 ccccggtcca tcctggggtt cgagccggga gacgaccgca agctgcccga ctggccgacg
     8761 ctggtccgct actaccgcgc cctggcggac gcgagtgacc gcgtcgacct ccgcgaaatc
     8821 gggaagagca ccctgggcgc gccgttcctc gccctggtga tctcgagccc gcagaacctc
     8881 aagcgtctcg accacttccg cgccatcaac gcgcgcctgg ctgatccccg caccctcgcc
     8941 aacaacaagg acgccgagca ggcgctgaag gacggcagga cgatcgtgat ggtgacgtcg
     9001 ggggtgcact cgaacgaggt gggtggacac ctctcgccgg tgcagctggc ctatcgcctg
     9061 gccacggaca cgagcgcccg aacccgcgcg attctcgaca acgtgattct ctggctggtg
     9121 ccgtctctca atcccgatgg cgtgacgatc gtcagcaagt ggtacaaccg cacgctgggg
     9181 accccgtcgg aagggagcca gccgccggag ctctatcacc attacgtcgg gcacgacctg
     9241 aaccgggact ggtacgagtt cacgcaggtc gagacccagc tggtggcgga ttcgctctac
     9301 ggtgtctggc atccacagat cgtgatggac ctgcatcagc agggacagaa cggttcacgg
     9361 ctgttcctgc ctccctacct cgatcccatc gagcccaacg tggatcctct gctggtggat
     9421 ggggacaacg cgctgggtac atcgatcgcc tggaccctgg gcggtgaggg gcgcaccggg
     9481 gtcgcgatca acagtctcta cgacgcctgg acgccgtctc gcgccttcgc ccactatcac
     9541 ggcgcggtgc ggattctcac cgaagccgcg agcgccgagc tcgccacgcc cgtcgaggtt
     9601 ccgttcgatc aactctccgc ggcggggcgc ggattcaacc cgcgcgagcg ctcctggaat
     9661 ttcccgcacc cctggcccgg gggccgctgg acgctccgcg acatcgtgag ctatcagacg
     9721 gacgccgcgt atgcgttgct cgagcaggcg tcgaccgacc ggaccagatg gttgagtgac
     9781 tttctcacgg tgggttggcg cggggtgcgc ggctggcggg gctggccctg ggcctacgcg
     9841 attcccaggc agcaggactc gatcgccctc gcgacgctct tctcggtgtt ccatcacggc
     9901 ggtgtcgagg tgcgttcggc ggaccaggcg ttcagcgccg gcggtcagcg gtacgcggcg
     9961 ggcacgtaca tcgtgacgtt gcgccagccg tatgccgctt tcgccaagac cctgctcgag
    10021 ccgcaggcct accccgaccg gcggctctac ccgaatggcc cgccggagcg tccctacgac
    10081 gtcaccgcga acaccttgcc gcttctgctc ggccttaccg cgacaccact caccgaggag
    10141 ccggcccggc tgcagttgtc ggcgccgatc gcggcgccgc atgcgacgcc cgggtacccg
    10201 ggattcggtt cgggagaagt gccgaaggtc gccatttatc gatcgtatgc cgcgtcgatg
    10261 gacgaaggat ggacgcgctg gattttcgat acctggaagg tcccgtacgt ctcgatcgtc
    10321 gattcgacga tgcgcgcggg cggactcaag gcgaagttcg acgtgatcat tctccccgac
    10381 gaatcgccgc acgacctgct cgaaggactg ccctcgccgc gctaccccgc ggcgtatgcg
    10441 ggcgggctgg gatcgagcgg tgtcgatgcg ctccggcaat tcgtgcagga tggcgggacg
    10501 gtggtggccc tcaacgagtc gagccgcttc ctcatacaga cgctgctgct cccggtgcgg
    10561 aacgtcctcg aaggcgtggg ggacgacgag ttctatgctc cgggatcgat tttccgtctc
    10621 gagctcgaca ccacggcggc ggaagctcac ggcatgcctc cgcggagcat cgcgtggttc
    10681 gaggggggac ccgctttcga cgtgctcgat tcggcgcagg ttcgggtcat cggccggtac
    10741 cccaccgacc ccaactcgct cctgctgtcg ggctgggtgt tgcaccctga gcgcatcgcc
    10801 ggacgtgcgg cgatggtgga ggtgaaggtg ggtcagggaa aggcggtgct tttcgggttc
    10861 cgcccgcagt accgcggtca gagcctggcc acttatcccc tgctattcaa cagcttggag
    10921 atgaagtagc agtttgtact cttgattctg ccggtgcgaa ttcctatcgt gttcacccct
    10981 atgcgccttc gccgtgaccg caggttgttg ttggtgctcg ccgccctggc tttcccgggg
    11041 cggcttttcg cccaagggag ggccgattcc acgcgcacgc tgatccccgt gcgcgcggat
    11101 cagctgccga ttttcctgca tcccggcacg attcacttcg cgcatcccga tcagcccatg
    11161 ttcgtgaacg cgcgcaacgt gaaagcgctc tacgtcaatg catgggcctt cgcgtcgccg
    11221 aagctctggg gcctcgcgcg cctcgcggac tccaccgaga tcaacgcgct ggtcgtcgac
    11281 gtcaaggacg ataccgggtg catgctgtat ccgtcgcagg tgccgaccgc catcgagatc
    11341 ggcgcgagcg cctgcgcgcg gaccaagaac gtcgaagctc gcctcgacac gctgcgtgcg
    11401 catcacatct acaccatcgc gcgcatcgtc gtggcgaagg atccgcggct ggccgagcgg
    11461 aaaccggagt ggtcggtgaa ggacaagcgg ggcgggctct ggcgcgaccg gatcaacatc
    11521 gcgtgggtcg atgcgtacaa cgactccgta tggatctacg cggcacagct cgcgcgtgaa
    11581 gccgtggaga agggcttcaa cgaagtgcag ttcgactacg tgcgcttccc cgacgaaccc
    11641 cgggcgaagc tggccgaggc gattttcccg gccaagaagc ccggggatac ggagcgggtc
    11701 gcggtccgcc gccacgtaca gcttctggtc gatcgggtgc gctccgccgg cgtgcccgcc
    11761 accttcgaca tcttcgcgct cacggcctcc accacggaag gggatctggg catcggccag
    11821 gtctgggagg atttcgcttc ggtggccgat gtggtgttgc cgatggtgta tcccagccac
    11881 tactatgctg gcgccttcgg gttcgcgcat cccaacgggg agccgtaccg catcgtgcac
    11941 gacgccctgc gggacgccca ggagaagagc cgcgacattc cgaacgcggc caccatccgt
    12001 ccgtacttgc aggcgttcac cctcggccgg cgcaagccgc gctacacgcc ggtggagatt
    12061 cgcgaagaga tccgcgcggc ggaggagttg ggcattacgt cgtgggtgct gtggaacccc
    12121 aggagcgtct atcagcgcga cgccctgcgg cccttccgca ccaccagcca cccctctgaa
    12181 accgaagccg gcgtcggcga gtaggactaa gcacttcaca acggagatgg gcatgcggaa
    12241 ggtcggcgtg gggatggtgc tcgcggcctg gttcgcggcg gcgtgctcct cgagcacgac
    12301 cagcgtgagc gcggactcga tcgacggcgc ggagctcgcg gcggtccggg cagcgctggg
    12361 caaggcgctc gcctcggaca gcttctatgc ggagctttcg acgttcgtat tcccgttcat
    12421 cgatcaggcg acgaaggaag gcgaagggcc gggcgacacg acccgcctcg tcgggatcct
    12481 gctcgacatc aacgcgacca agggggacac cccgatcgtc gcgcgtttca gcgccctgct
    12541 ggactggcgg cactatcgcc ccacgtccca gacggtggac agcgtcacct tcctgctcgg
    12601 tgccgggatc acgcccccca tctcggactc gctggcgaca tccttctcac cggactcggt
    12661 ggggaccggc gtcggcttcg tggtcgccca ggcgcaggat tcggcggtgc agacatggct
    12721 cacccgcgcc ggcgcgtttc acctgaccgg cgccagtttc ggctcagctc aatccacggg
    12781 gctgggtggt ctcacgctcg ccgtgtcaca cggtacgatg aatggggatt tccatctgac
    12841 ggccaagctc gagcccgact cttcgacgac ggtgacgggc acgcgcagct tttcgggggg
    12901 aattcacgcc ctcaagatcg ccatcaccgg ctctttgagc gcgcctccgg cgccggcgcc
    12961 cactcggtaa tcacccccgc gatggccgac gcggccaccg tgtagggatt cgcgaggtac
    13021 agctggcccg ggccggagcg gcccggaaag ttccggttga tggccgagat ggtgacttcg
    13081 tccttggtgc gcgagacgcc gggacccgca ttgatgcagg cgccgcagga cggctcgagg
    13141 aacgtggctc ccacctgccg gaagatgtcg agatagccct gctcttcgca gtaccgcttc
    13201 acgtcctgtg atcccgactg aatgaagaac ttcacgcccg gcgccacctt ctggccccgg
    13261 tccaccgctt ccttgaggac ccgggcatac atgtccatgt cctctttttt cccggcggtg
    13321 caagaccccg cgtacgcgat ctcgaccttg accggcgcgc cgagctcggc gatgtaggcg
    13381 ccgttgcccg ggtcaccggg gagcgcgacc atcggtttga gccgggaagc gtcgatctcg
    13441 atcacctcgc agtactcggc gttcgggtcg gaggctattc cctcgcaggc cttctcggcc
    13501 tcggcgcgcg tcatcttccg gtagtccatc agatacgcga ccgtcttctc gtcgggcgcc
    13561 acgtaccccg tgaacccgcc cacctcggcg gtcatgttcg tcatcgtcgc acgctcgtcg
    13621 accgagagcg cctcgaccgc gccgccgcaa tactcgacca gcttcccgat cgcctgcccg
    13681 ctcttgatgt acggatgccg caggatctcg agcatgtaat ccttggcggt cacgttcgcg
    13741 ggcttggcac ccttgaccac gaccttcacg gtcgcgggca ccgtgagacg aacgtccttg
    13801 gtaatccacg aattgaagat cgcggtggtc ccgacgccga aggcgacgca ccccacggcg
    13861 ccggcgtgcg gggtgtggga atccgagccg atgatgacct ggccgggaag cgcgtgagac
    13921 tcgaggatct tcgagtggca gatggcctcg gaaccatgat ggcccaggcg cagctccccg
    13981 tagagcttga tcccctgttt ctccgcgaag gcgcgctgct tcttctcgag ctgattggcg
    14041 acgtcgagga ggccttcctt gatccgctcc ggcgtcatcg cctcacccag gaaggtgagg
    14101 tggtcgcgga acaggagcac cgagccggga tcgagcacct tttcgtcctg gccgacgagc
    14161 tgttcaaaga agatggcggc catcggagtg acgtactcgt ggctgaaccg gatgtcggtc
    14221 accacgaagc cttcgtcccc cggcttcacc gacggaacgc cgaccttgcc cttggacgga
    14281 tccacaaccc agtgacgggc cagcaccttt tcgccgaggg tcatggggcg gcgcggcgtg
    14341 gggggcggac tgaggacgac tttcccctga aggcgcgcca cgttgtagtt gaagaggccg
    14401 ccgtactcga tgatgcccct ggtgatttcg ccttcgccct cggtgaacgc ggagagcggg
    14461 atctcctcgc cccggcgaac gcgctcgatg atcgagaaat cggtcgtggt caggacgccg
    14521 aggttctggc aattctcgcg atagatgcgc tcgatgtttt cggcgacgac cagcttgatg
    14581 ccggcgcaca tctcggcgta gggcgactgc tcccgggacg accccttgcc gcggcgcttt
    14641 ccggagactg aggcgacgaa accaccctgc ttgaccgatc cgcgggtgat cgggaactgg
    14701 tcaccgcact tgagtccgag ataggggaat tcccccagcg tctcgtcgta gtagtagcag
    14761 atgtaggcgg gcgtgatctc gtcggtcgag atgttgtcac gcagtggcac cttggggggc
    14821 cacggcaggt ccttgcccgc gagctgctca cgcacaagcc cggcatcctc ggtgaggaac
    14881 aggatgcggc ctgggaagcg gacccgatcg ggcagcttat tgacggggcg agacttgaga
    14941 gcttccagca tgccgaaaaa tgaccccttg ctacaggtgg agcaaggcga ttggttcagc
    15001 cgatgcgacc tctatacacc ctcttgttga tcttcggggc ttcgtcgggt cccgccctcg
    15061 cgcaaaaacc cgccactccc gccacaccgg ccggcccgcc gcccccgagc atcccggcgc
    15121 cgaacgagcg tgcgatggat gcccatctcc ggttcttggc gagcgacctg ctggagggac
    15181 gcgccccggc gaccaggggc gggcacctcg cgaccgatta catcgccgcg cagttcgaag
    15241 cgttgggcct cgagcccgcg ggtccgaatg gctcgtattt ccagcctgtt tcgctcgccg
    15301 gaaccctgac ccagcagccg tcattcgtgt ggggaaaggg cgcggacacg aggagcctca
    15361 agccgggcga tgagttcgtg gagtgggcca tgaagcccga gtcacgcatc accgtggatg
    15421 gtgacgtggt gttcgtcggg tacggcatca aagcgcccga gtaccgttgg gatgactaca
    15481 aaggcgcgga cctccacggc aaggtgctcc tgatgctcgt gaatgacccg ggcatccagg
    15541 actcgacgct cttcaaggga cgggtgctca cgtattacgg gcgctggacc tacaagctcg
    15601 aggaggcggc ccgacagggg gcggtcgggg tggtcctggt gcacaccacg ccgagcgcga
    15661 cctacggctg ggacgtggtc cggggatcct ggtccgtcga gcagttccag ctggagggag
    15721 acgcgggccc ttccctcgat tttgcctcgt gggccaccca cgacgcggtt caatccgcgc
    15781 tcgcatcggt ggggctcaat ctcgattcac tcacccggct cgcggccacc cgggggttcg
    15841 tgccggtgtc gaccggcctg cacgcggcga tcgacctcgc gagtgcggtc cggcacgtcc
    15901 agagcgagaa cgtggtcgcg cgtctccggg gctccgatcc cgtgctctcg tcacaggtcg
    15961 tggtgatttc ggcgcactgg gatcacaagg gcatcgggcc cgcggttggg gacgattcga
    16021 tctacaacgg ggccgaggac aacgcatcgg gagttgcggc gatgctggcg accgccgagg
    16081 ccctgtcgaa gatgccgaag cgccccaggc ggtcggtgct cttcgtggcg acgaccgccg
    16141 aggagagtgg gctcctggga tcggcggggt acgtggcgca tccgctcttt cccctcgcgc
    16201 aaaccgcggc cgatatcaac atggacgagg tgaacgtgca cggggcgacg cgggatatcg
    16261 ccgccctggg cactgatcag tcgtcgctgg ggaagatgtt cgcggacgcg gcacaggcgg
    16321 agtcgttgaa ggtcgccaat cgtcccgaca tcggcggggg attcttccgg tccgatcact
    16381 tccccttcgc gaaggccggg gtgcccgcgc tgtcgattca agcgggggag gatttcatcg
    16441 ggaagcccgc tgggtggggg agacaggagg agcaggactt cgagcagcac cggtatcatc
    16501 aaccgtcgga tgagtggact cccctcttcc gctatgacgg aatgagccag gaggtccgag
    16561 tggtgatgcg cctgacactc gcgatcgccg acgcgaccga cctgccgcag tggttggcgg
    16621 gtgccgattt caagcgctag ttgtgcagat tattgcagta cctaggagtt tgcgtaacat
    16681 gctatcataa aataagttgg cggctggggc cgcgtgagtc attgctggac tcgcaggggt
    16741 caggtaagtt tgcgcccgtt tttgacggtc cggcaagcgc aaacgtcagt cctggagaat
    16801 gaccctggaa ctccccacca cgcatcgtct tccgctatcc tggctcaagg atgcagccag
    16861 tcttccgatt cagtatcgag cgttggctga ggctgcacct gaaggagctc gtgatccgga
    16921 gcgtctggcc gagctcgcca agcaggccct cgagtacaag cccgcccagg cgatcgcgcg
    16981 gaagcagaag gacaccggac tgtggggcgc gaacctgctc gcaccgggac ccaacaaggt
    17041 gttcgggtgg accgagacgg ggaccgtcac ccagtaccgt cacttgctcg agctgggctg
    17101 gcccgccgat cagcgcgtgt tccgcatcgc cgatcggttc ttgttccgcc tcctttctcg
    17161 cgaccccgat cccaccctgc tggtcgagtt tcagcgcccc gccaagaccg acccgggttt
    17221 gggtctctgg gcgcgcaacc tcggccggga ggctgcggcg gccgcgctcg cccgggcgtc
    17281 acgcggcgag gacccccgac tgcgcggagc ggcccacaac atcctgaacg ggatttcgac
    17341 ctacctcaga agtcccctcc tggaaaagcc gttcaagaag gcccagggca agacggtggt
    17401 cgagcctgag gcggtgcccc cttcgtactt cgcgatggag atgctggctt tcctgcccgc
    17461 cctccaacgg gagcgttcag ggttcatcga gcggctcggg gcgtacttct cgacgccggc
    17521 gcccaagcgg gcgttcttcc agttggcggg aaaaaaggtt ctgcccccca cgttcatcat
    17581 catgggtgac cccctccggg cggacgctca cggtcacgta ccggatgttc cgttcgccgt
    17641 gtactggctg gaaatcctcg cccggctggg tctggtcagg cagctcccct ccgccaccaa
    17701 ggtgctcgcg cgcctctact ccgagtgcga cgaccagggg atctggagtc ccaaggcgct
    17761 ccggaccatg ccgaagtccg acagtccggt cctgagccac tacttcccgc tcgaaggacc
    17821 ggggaagagt ccggcccaga agcagaccga catcacgttc cggctggcgc tcatcgcgcg
    17881 actgctgggt ctcccgatcg aagtcgtcta gtttgcggag atggtcatag agtagtagct
    17941 ttggctacta ctctatgacc tcgatcgacc tcacaccgtt cggcttcacc cccaccgagt
    18001 ctctcgtcta cgcctccttg ctccgcctgg gtccgagtac cggctacgcg gtcgcccgcg
    18061 cctgccgtct ggcccgtgcg aatgcctatg ccgcgctgga aggcctggtg agccggggcg
    18121 cggcgacttc cgcggggcgg cccgcccaat tccgccccgc cgacccttcg agcctggtgg
    18181 ctcgggtagc agcatcccag gggcaggcgc tcgaacggct gagccgcgcg ctcgagggcg
    18241 cggctgcggg gtccgagccg acgattcact ctgccgaggg tctgcggggg acggtaaatg
    18301 cggtgcagca gctggtggct cgcgctgagc ggagcgtgag gggaacggtg gcggcggagc
    18361 tctggcgtcc gtccctcccc gcttggcggc gcgcggcaac gcgggcgtcg ctcgacgttt
    18421 tgatcgccgg agaggctgaa gacccggagt cactcgccag aggaacggca cctgcctcgt
    18481 ctgcgaccat tctgttgatc gatgaagttc gggtgttttc cgcgtcgggg gcgggcgatc
    18541 tcatgacggc catgtggacc gcacatcccc tgctggtgca ggtcggatcg gcggcaatga
    18601 ggtgaaaacg ccgccgttgc tggggtttct ggccctgggc gcagtgggat gccgggccgc
    18661 gccaattcct gaatcccagt ggtatcccgc agggaccaac ctccgggcca agtacgtcac
    18721 cgtccagggc acccgcattc ggtacgtcga gtcgggcgcc ggtccgacgg tcgtgcttct
    18781 ccacggactc ggcgcctcga tctacacctg gcgggacgtg atcggcccgg tcgcggccgc
    18841 cggcttccac gtggtggcgt tcgataaccg ggggtttggc ggatcagacc ggccgacctc
    18901 gggctacagc gccggcgact acgcatcgct ggccgtcgcc tttctcgact cactgcacct
    18961 cagtgacgtg gtattggtag gccactcgat gggcggggag atcgcggcac tcgcgaccct
    19021 gaaagatccg tcgaacgtcc gcggcctggt cctgatcgat gccgcggggc tcggcactca
    19081 cctgcccctc cttactcggc tcgcgcgagc tccgctggtc ggccacgtga tcgccgggct
    19141 acggggacga agtggagtcg aaagggccct gcgatcgacg tttggagtcc ccagccgagt
    19201 gactcaggac gaagtggatc agtactacgc gcctgtggcg atgcccgatt tcggcaggac
    19261 gctggccggg gtcgcccgcg agttcgattt cagcgccctc aagggacggc tcgattccat
    19321 cgcgaagccg acgctcgtgt tgtggggctc ccgagatatc tggattccgc ccgggattgg
    19381 ggagcgcatg acggccagcc tgcagcgggc agccttcgtc ctcgtgccgg gagtgggcca
    19441 tgatctgccc gacgaggacc ctgccgccct gctcaggtcg ctgatcccgt tcctgcaaca
    19501 ggggattcct cagccaccgc cgaacgttgc aatacttggt attgatatca accagcaggt
    19561 tgataaattg gagcaatgaa gcataagctc agtaattcga gagccttacg aatcatcgat
    19621 tcggcacagc gggagttcgc tcgtgagggc ttcgagggag ctcgggtcga tcggatcgcc
    19681 aagtccgcag gggtcaacaa gcagctgctg ttctactact accagtcgaa gcaggggctc
    19741 ttccgtgcgg ttcttggccg ggcggcggca gacctcgagc agtcgctggc cgggttgacc
    19801 gcgagtgggg gccgcccact cgaccggctc gagcgctccc tggacctgct ctacaacttc
    19861 ttcgccgcgc accccgagct ggtggcgctc ctcacccgct ccgggcgggt cgacatcgcg
    19921 ccctttgctc cggtggtcaa gcgactcgtc gtattgctgg cggaaggtca gggcctcggt
    19981 caggtacggg atgatgtcga cccgcacctg accgcggccc aagccctggt cctgatggta
    20041 ggctttctca atctcgagtc ggtggtcgcg gccagcgcgc cttcactcgc gatggatgaa
    20101 cccgcacttc ggcaacgctg gcgcgagggc gccactcgac tcctggtcgc cggggtcgcc
    20161 gcgcgctagg acttggcgtc ctagcgtcca accatcctgg gctccttggg agcatcaccc
    20221 tcgaacacca ggtgacccgc ctcaaagcgc gcgccccgca gcgccaggct caacacctgg
    20281 cccagatccg acaccaggtg cacgtccagc atctggcgaa cctcagccgg caggtcctcc
    20341 agatcccggc cattctcctg gggcaggatg atggtctgaa tccccgcccg gacggcgccc
    20401 agcacctttt ccttaacgcc gccgatcggc aggaccttcc cgcggagagt cacctcgccg
    20461 gtcatggcca ggtcgtggcg cgccggtctt cccgagagcg ctgagaccag ggcggtcgcc
    20521 atggtgacgc cggccgaggg cccgtccttc gggatggcgc ccgcgggcac gtgcacgtgg
    20581 acgtcggaat cttggaggcg cccctccgga atcccgagct ggtccgcatg cgacttggcg
    20641 taggtccacg ccgcgcgcgc cgactccttc atcacgtcgc cgagctgtcc ggtgagggtc
    20701 agctcgccct tcccgcgcat cacgctggcc tcgacgaaca tgatgtcgcc acccaccggc
    20761 gtgtagtaca tgccggtcgc gacgcccacg tcgtcggcgc ccgccttgcg ttcggggtga
    20821 actttcggcc gtccgatgag ggccggcacg tccgcggcgg tcacgttcac caccaccgcg
    20881 tccttgccgg cgatcttccg cgccgccttc cggcccacct tcccgatctc acgctcgagc
    20941 tgccggacac cggcttcccg tgtatagctc gagatcacga cctgaagtgc gtcgtccgtg
    21001 atcgtgagtt gttccgccgt cagcccgttc tcctggatct gacgggggat gagatagcgg
    21061 cgcgcgatct cctgcttttc gcgctccgtg tagccggcga acgtcaccgt ctccatccgg
    21121 tccatcaacg gtcccggaat gttctggggg aagttcgccg tcgcgatgaa cagcacttcg
    21181 gacagatcga acggcacgcc cagatagtgg tcggtgaagc tgtcgttctg cgccgggtcc
    21241 aggacctcga gcagcgccga ggcgggatcg ccctggaacg agacaccgag cttgtcgacc
    21301 tcgtcgagca ggaagaccgg gttcttcgca cccgcctgct tcatcccctg gatgatgcgt
    21361 ccgggcatgg cgccgacgta cgtacggcga tgcccgcgaa tgtccgcttc atcccgcgcg
    21421 ccgcccagcg aaatgcggac gtacggccgg cccatcgcgc gggcgatgga cttcgcgatc
    21481 gaggtcttgc cgacgccggg aggaccgatg aagaggagaa tcggaccctt ggcttgcttg
    21541 tcgtcgtcgt tccccttcgc ttccgtgtcg ggcttctcga gtcggagctt ccggaccgcg
    21601 agaaattcga ggacctggtc tttcacttcc tggaggccga agtggtcttc ctcgagcacc
    21661 ttcgcggcgc gctcgaggtc gagggactct tcggaacgct tgttccacgg cagctcggcc
    21721 acggtctcga ggaaggttct gatgacctga ccttccatcg aatccttgtt ggcgcggtcg
    21781 agccgcagca gctcgcggtc gacttccttc cgcgcggctt cggggagatc gagcgcggcg
    21841 atccgcttcc gcagatcgtc gacgtcgccc gcttcgtctt cgtcccccag ctccttgtga
    21901 atcgccttca gctgctcgcg gaggtacatc tcccgctgcc gttcacccag ctcttcctgg
    21961 acctgggact tgatgtcctc ttgcgcgtcg agcatgccga tctgccgctg gacgtgcacg
    22021 agcacgcgcc ggagccggtc ttcgacgccc agggtctcga gcaggctctg tttttcgctc
    22081 ggcttgacct cgaggtagcc ggcgaccagg tcggcgaacc ggcccggctc gttgacgtcg
    22141 tcgaggacct gtttgacgac ctcctcgggg aggcctgatt tccggcccag gtccccggcg
    22201 cgctcccggg cttcccggta gagcgcaacg aaggcctggt tctgctcatc caacggaggg
    22261 aggtcggtgg cttcccgaac cacggcctcg aggtgcccgg acgcctcctg gtagtgaaga
    22321 gccgtcccgc ggacctcgcc gtggagcatc agctggaccc caccgatgcc acgctggatc
    22381 tggctgattt tcgcaatggt accggtggta tagaggacgc ccggcgtcac ctggtcgacg
    22441 ttttcgcgtt gggaaacggc gaagatcagg tgggactggc tccgcaaggc ggcttcgatg
    22501 gcccgcaggg tggcaggcct cccggccccg atgggctgag acacaccggg gaagaggacc
    22561 atttcgcgga ggggcaggac cggcagagtt agacgctcgt acatcgcaat cgactccatc
    22621 tttgaaagag tggctgggca cccgcaggtg catccaccgt gccaaggcaa gaaccgcttt
    22681 ccaaggcgac gcgttccctc acttcccggc caaagaggca ggacctgccg aagcgcgcct
    22741 gaaggtgtgc cccgagcggc cagttcggcg gagaaacgcg tgccgcgaga ctccgtaagt
    22801 agcgatggct agcgtggttg ccggacaggt tccagagggc taatctttca agtctgtact
    22861 cacgtgcgtt ttagcctgag ctctagaggg gaatgagcga gaacgacaac tcgttaatgg
    22921 gccgcctgtc ccgggcgctc ggttccgcct atgcggtcga cagtgagatc ggccggggcg
    22981 ggatgggcgt tgtgtacagc gcccgcgacg aacggctcaa gcgtcgggtg gccatcaagg
    23041 tcctcccgcc cgagctcgcg ttccgcgagg aaatccggct ccgcttcctg cgggaagccg
    23101 aaaccgcggc gcgtctgtcg cacccccaca tcgttccgat tttctcggtc ggcgagggtc
    23161 cggacggcct cgtctacttc atcatggcct tcgtggacgg ggagccgctc tccggaaaac
    23221 tccagcgccg ggggactctg cctcccgaag aagcgcgccg catcatgctc gagacggtcg
    23281 acgccctcgg cgccgcccac gctgttggga tcgtccatcg cgacgtaaag cccgacaaca
    23341 tcctgctcga agggtcccgt gggcgcgtga tggtcacgga tttcgggatc gccaaggcgc
    23401 tgacttcctc cacgggcccg ggcacgctga cgggcaccgg cgtggccatc ggtacaccgc
    23461 actacatgag cccggaacag gcgctcggcg agcgggagat cgacggacgg tccgatctct
    23521 acagccttgg cgttgtgggc taccagatgc tggtcggtga gctgccattt tccgcgccga
    23581 cagtgcccgg tctcctgatg aagcagatca cggaagaagc accggacatc acgtcgaagc
    23641 gctccgattg cccctccgag ctctcggcgt gcgtgatgcg ctgtctggac aaggatccgg
    23701 agaagcggtg gccgacggcc gacgcgctcc ggcgcgcact cgaatcccgc tcgacgccgg
    23761 cgttccgccc ctcgccgcgc gtggtcacga gccgcgggtc gagcgggctg aatcgtcccg
    23821 ccgctcccca gggccgtgcg ccgctgcccg tgccgcgttc acccgacgtc ggccgcggcc
    23881 gctccctccg ccgccagcag cgcgaccggg accggaatca gccccagaag actcctggtg
    23941 gggaggccgc gatggtgaaa gccacgcgcg ccaacttcgt gtcgtgggcg tccgtgtcgt
    24001 gcggcctgct cttcttgaat ctcgtcaccg gtggcggagt gggctggtcg atctggccca
    24061 tcggcttgtg gggggcgttc ggtgtgctgc cgcgcttctg gcgcctctgg gaggccgggt
    24121 acagctggcg ggacgtcttc atgcgccccg ccgctcctga tgccgcggag tcacgactct
    24181 cgccggcggt gggcacgccg ctgatcccgg cgagtaccga cgaatacggc aacgaggcgg
    24241 ccaccatcga ccaggtccgc cgtgaccggc aggcgatctt cggggtggtg cagcgcctgc
    24301 cgcaatccga caaggagcgg ctgcccgaag tcacccagac cgtcgacgcc ctgatggcgc
    24361 gcgctgagaa cctcgcgaag atgctgcacg cgatgagcgc cgatgtggat ggaacgagcg
    24421 ttccccggtt ggatgaacgc attgcccagg tgaaagcgca gcccgagggg tcggagcggg
    24481 accgccagct tgggctgctc gaacggcagc ggcaggcgtt ggcggatctg gtgggacgcc
    24541 gggacggggt gcgggaacag ctcgagtcct gtgtcctggc aatgcagaac gtccggtatg
    24601 acctgctgcg cctcaagtcc gcggggatcg gcgccgtcct cggcgacctc acccaggcca
    24661 cccagcaagc ccgggccctc tcacgcgacg tggaccacgc catcgccgcg gccggagaag
    24721 tcaaagacct gatgcggtct tagaaggcct tcagctcccg catcgccgcc gccagacctt
    24781 ccgactgcgg cagaatcaca tcctccagct caggcgcata cgcgacgaac gtgtccgtcg
    24841 aggccacgcg cttcaccgga gcgtcgagcc acgggaagca gacgtccgcc accttcgcgg
    24901 cgatctcggc gccatacccc cacgacagcg agtcttcgta ggcgaccaga accttgttgg
    24961 tcttccggac cgactgttcg atggtctccc agtcgaccgg tgagagcgac cgcagatcga
    25021 tcacctcgac ccgcgtgccg tccgcgtctt cgagcatctt cgcggcgacc agggcccgct
    25081 gcaccacggc gccgtaggtg atcacggtca ggtcggtgcc ttcccgcgcc actcgtgcct
    25141 tcccgaaggg gatcatgaag ttcgggccgg ggttcggggc cttgttgtag gtctggcggt
    25201 acaaatgctt gtgctcgagg aagagcaccg gatcgtcgca gcggatggcg gtacggagca
    25261 ggccgttggc gtcgagcgcc gtggacgggc agatcacccg gagccccggg accgacgtga
    25321 acaccacggc gccggtctgc gagtggtaga tcgagccacc cttcagatac ccgccgtacg
    25381 tcacgcggac caccgcgggg catttgaagg cattgttcga ccgccaccgc atggtcgcca
    25441 gctcgttccg gatctggtgg tacgcgggcc agatgtagtc gaagaactgg atctcgacca
    25501 ccggcttcca gccccgggtc gacagtccga tggcgcgccc gaggatgttg gcttcggcga
    25561 gcggtgaatt gaagacgcgg tccccgccgt actggcgctg caggccccac gtcaccttga
    25621 acacgccacc cttgcccttc acatccttga gcaccgcctc acggctggca tcggcgacgt
    25681 cctcgccgaa gacgagaatg cgcgggtcgc gcgccatctc gtccttcatg caggcgttca
    25741 ggaggtcgac gaccgtcgtc ggctcaccct tgaactgggg atcgtcctcg gtgtcgaact
    25801 gctccgacgt ggggtccacg tcgggggagt acacgtagtt gtagacggtg tcggtcttcg
    25861 gctggggcga caccagcgcc atgtcggccg cgacctggac gtcgtcgtcg acctgcttct
    25921 gaatcgcctc gatctccgcc tgggtcgcga tgccgtccgc cacgagcttc ttggggaaga
    25981 cgatgacgca gtcgcgcttg gcctcttcct cgcgctcctt ggtggggcga tagaggattt
    26041 cgtcgtccga gagcgagtgc gaataggggc gggtgacgtg ggcgtggacc agcgcggggc
    26101 ctttgcgctc gcggcaatac tccaccgcgc ggcgcatcgc ctcgtacgac gccaggggat
    26161 cgcagccgtc gacttcctga atgaagaggt ccgggaaccc ggtgaccagt ttggagaccg
    26221 agccgccggc cgtctgaact tcgaccggca cggagatggc gtagccgttg tcttcgacga
    26281 ggaacacgac gggcaacttg agattgcacg cggtgttcag ggcctcccag tactcacctt
    26341 cgctcgtggt cccgtcgccc gacgagcagt acaccacttc gtccttcttc accgacacgt
    26401 cggcgtcggc aatcttcgac gcccgatacc aggactcggc acagccgacc gcctggatga
    26461 actgcgtccc ggtcggagag gactgactga cgatgttcag cgcctggtgg ccccagtggg
    26521 acggcatctg ccggccaccc gaattggggt cctcggcggc gcccacggcc gagaggagca
    26581 tttcggtggg ggtcatgcca agggtgaggc agagggcccg gtcccgatag tagggataaa
    26641 accagtcgtg actgggcttc attgccttcc cggcggctac caggatggct tcgtgcccgg
    26701 cgcccgagat ctggaagaag atcttgttct gacgcttgag ctggatctcc ttgtcgtcga
    26761 gacgacggga caggagcatc agccggtaca tgtccaggag ctcagccttc gagagctggg
    26821 acttggcgga cttggtatgg gcgcgggact gggggggggt cttggtggcc ataggcctga
    26881 aaaatagcct acttgggctt cgccttcaaa aggtcgctca gcctggggaa ggcgccgagg
    26941 gccaggcgga cgtcccgatc gtgatggagg tccagatcat acatcgcgtc cacgccccaa
    27001 cggttttcca ccacccggcg gcccacttcg tagcccaggc gccattgggt ggtcgagtcg
    27061 accatggcct ccacgtgaag ccggtcgcgg atccgcttca ggaggccggg catgagggtg
    27121 ttgccccagc ggggcagcga ggcttcgaag gcggccattc ccgcgggcgt ggcaggctga
    27181 gtcagtgcca ccgagtcgac cacggcggtt atccaccctg agtcctgcgc cgccgcccac
    27241 caggccgccg gctgtacccc gccggagtcc acgatgtcgg ggagcactcc accgccacct
    27301 cgtacggcgc gaccggcatc ggtgtggtag accttgtcgg tgtcgttcgc cgctcccgcc
    27361 ttgccggcca gatcgtagta ctgctccacg gccaggcccg cgtaccggcg ctggatatac
    27421 cggcccgatg gcgagatgac gtggccgatg gtcatatgca ggacctcacc ggatgacagg
    27481 atgaagttgg actgcatcaa cgccttcccg aaggtccgtc ttccgatgat cagagcccgg
    27541 tcatggtcct ggagggaggc ggcggtcgcc tcggcggcgc tggcggagag ctggttcacc
    27601 agaacgatga ggggaaggtc cctgaagtcg ccgtccttgc gggtgcggta cgtcgtgtcc
    27661 acgtcggccc gccggcccct ggtgctgaag acgatcgtgc ccttggggac gaactcggag
    27721 accaggtcca ccgcctggtc gacgatgccc ccggggttgt tgcggagatc caggatgatc
    27781 tgcttcgctc cctgcttctt cagcgacttg atggcgtcgt gcacctgatc cgcggacttc
    27841 tcgccgaatt cgtcgaggcg caggaatccg gtgaccgagt ccaccatccg caccgaccac
    27901 acgtctcgtt cctcgaatgc cttccgcttg agggttacgg tgaaggtgtc gggctcgagt
    27961 ttggggccgc gcgcgaagcg gacgcggacc ttggttcccg gggcacccgc catgcggagt
    28021 cgcacggttt cgatctcgag gcccgcgatc accgtgtcgt tgatggcgag cacccggtct
    28081 cccggcctga cgcctccgtc ttccgcggga cttttcgggt agatgccgag cacagtcggc
    28141 tcgccctcga cgtcgtcgag cgacgcaccg gtggtggcca ggtcgccgcg ctggagcgaa
    28201 tcgaagcgct cgtagtcgag gcgggaaaag aacaggctgt gggggtccag gtcccgaagg
    28261 acgccgtcga tcgcggactt gaccaggatg ctgtagctga ccgagtccgg gtagctgtgg
    28321 aagatgtagg tcagcacttg agagaaggtt tgcagctcct cataggacgt acgctgctgg
    28381 gccgcgcccc cggtcaccgg cgcggccgcg agcaaggcga cgagcagggc cctagcgcag
    28441 cgagtcgagg cgtttcttgg catcggcgac ct
//
LOCUS       DASUBP010000213         6527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_88374, whole genome shotgun sequence.
ACCESSION   DASUBP010000213 DASUBP010000000
VERSION     DASUBP010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6527
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88374"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VFU75_08245"
     CDS             <1..769
                     /locus_tag="VFU75_08245"
                     /inference="COORDINATES: protein motif:HMM:NF019911.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HEU5466867.1"
                     /translation="YATMEDFATAVFPEQPVAAGRSGGPITPKPVARAPVTAESPTEA
                     FISAPTTPIPSGLRPPAGAGAPTVPTRPGAKPAPPPQKSGSRAWMGIAAAVVVLGVGG
                     YLVFGRGPKATNDGGQTAATQQAPSNPGASNPATTPADTHVVVTPPVTPPQQTASQPP
                     AQATHKPAPAPHRNPAPQPKPAAAATGFISIASDPIAMVYIDGQAVGQSPVINHEVPV
                     GSHTIRVEQVGFAAQTQTVQVSSGQTSFRKFTLLPGG"
     gene            772..1806
                     /locus_tag="VFU75_08250"
     CDS             772..1806
                     /locus_tag="VFU75_08250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466868.1"
                     /translation="MRRLRVILSLAASAAAAPLAAQGTAPQNPTLRRAVQAFDNLNLP
                     QAISLAQQALGQRLGPGDQEQAYTLLGSAYANQPDGFDKAMNSFKQVLLINSERVLDP
                     SRTPTKTLVAFNMALSTMLLVRGLQVDSSSFVVGQGAAPIHFTVTSPAHVRVRAVSNR
                     ASVTIDSTIENGTVNIRWPATLANGDPLPAGDYTVVVDVTAEQNSFSSSQRVHLAAGA
                     VDTLPHLTSVPGYSKLPDTEVPPRSWRPLGVAFLYAGIAGAGTLALENSQLGSANRRE
                     LSLVAVGVLATGVVMMLKKPSPVPAQGNILYNKLLDEQLIRRNADIATQNVTLRRQVR
                     LTVTPIPAGK"
     gene            1803..2450
                     /locus_tag="VFU75_08255"
     CDS             1803..2450
                     /locus_tag="VFU75_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466869.1"
                     /translation="MKRALIAVALCAFAAPASAQFVNVGVQGVFADYRELGPGLSYNG
                     GGLGGIAELNWHKFSGDFQIAKINYNPSSGSAATDKFTSTQFDAHLRWFLANGVSLET
                     GYTKRDISPDFTTQNFTAIRAGVRANYGLGPGVNLLLRGNYLAAGKFSGGGTAPFALE
                     LGLGLSVGKANNRYRFTADYEFQRIDRKTTISGTDYKVPIQQAMARVGAAIGLGR"
     gene            2631..3320
                     /locus_tag="VFU75_08260"
     CDS             2631..3320
                     /locus_tag="VFU75_08260"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEU5466870.1"
                     /translation="MSDSRSAVVLGATGAVGSSVVRALLADDAWKRVTVLVRRPDALK
                     DFKDPRLERHVVDVLDAGQYERFLAGHGAAFCTMGLGEPSKCPREEFKKVDLDGTATF
                     AAACRRQGVTHFLLLTAVGANARSSIYYAHIKGEVEDRVRGLGFARASFFRPSMLMTP
                     TNRYGFTQGVFLKVFPFIDPLLVGPLSRFRSITVSDLGKAMVRHAERPGAGVEVLEWK
                     EFQAVLRGTGA"
     gene            3381..3818
                     /locus_tag="VFU75_08265"
     CDS             3381..3818
                     /locus_tag="VFU75_08265"
                     /inference="COORDINATES: protein motif:HMM:NF024297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5466871.1"
                     /translation="MKRPALVIALLVLASIPLRSQTPPSGDESAVRAVVERYLHGLKF
                     NDTTALREAFWPDAKLYYVRGDSLAQWTQSSWYASFAGSVGHEEQGDLRIASLEVTRD
                     IATAKIVEEYPKSRYTDYVSLLRIRGRWWIVNKVYTAERRKEG"
     gene            3838..4221
                     /locus_tag="VFU75_08270"
     CDS             3838..4221
                     /locus_tag="VFU75_08270"
                     /inference="COORDINATES: protein motif:HMM:NF037170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5466872.1"
                     /translation="MAKVTGIGGVFFKSTKDHKALAAWYQKHLGMPLESWGGAMLKWP
                     EDRAGDDGVTVWHVAEPNTKWFAPSTASFMINYRVDDMTGLLANLRAAGIEIIKGPDT
                     DDNGTFAWIVDPDGNKVELWQPKAQ"
     gene            4200..4472
                     /locus_tag="VFU75_08275"
                     /pseudo
     CDS             4200..4472
                     /locus_tag="VFU75_08275"
                     /inference="COORDINATES: protein motif:HMM:NF023542.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
     gene            4455..4871
                     /gene="arr"
                     /locus_tag="VFU75_08280"
     CDS             4455..4871
                     /gene="arr"
                     /locus_tag="VFU75_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)--rifampin ADP-ribosyltransferase"
                     /protein_id="HEU5466873.1"
                     /translation="MRVTGEVTDWLLYHGTRADLKPGDLIKPGHTPNFGDRTRTHAWV
                     YFARTLDAATWGAELASGDAPGRIYVVEPTGLVEDDPNLTDKKFRGNPTKSYRSRDPL
                     RVTGEVKNWKGHPPEALKAMKEGLKRLKGQGAEIID"
     gene            complement(4953..5561)
                     /locus_tag="VFU75_08285"
     CDS             complement(4953..5561)
                     /locus_tag="VFU75_08285"
                     /inference="COORDINATES: protein motif:HMM:NF025006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HEU5466874.1"
                     /translation="MRVPLLALGLLSTSFVRAAAQSQAAVKDSTQCVVADEHRSPRSF
                     NHDQDSDHNRVSDHDGDFDRDQRSDHDRKGDHHDGRRGCSPVTPPPPPPPPPPPPPPP
                     AGGASITGQVRDVTAGSGLAGWTVQVFGPTNASVITDANGNYTFSGLADGTYVVCEVL
                     QPGWTQVSPTFPESCASGMGYNFSVSSTSSASFVNFGNQTAL"
     gene            complement(5841..6146)
                     /locus_tag="VFU75_08290"
     CDS             complement(5841..6146)
                     /locus_tag="VFU75_08290"
                     /inference="COORDINATES: protein motif:HMM:NF013691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEU5466875.1"
                     /translation="MRTYYVYIMANRARVLYVGVTNDLARRVEEHKRGRVRGFTSQFH
                     VTQLVHFEEFVYIRDAIAREKELKGWRRSRKIELIERQNLAWQDLAERGFRIQSPSR"
BASE COUNT         1160 a         2100 c         2143 g         1124 t
ORIGIN      
        1 gtacgccacg atggaggact tcgcgaccgc cgtcttcccg gagcagccgg tggcggcggg
       61 ccgctcgggt ggcccgatca caccgaagcc cgtcgcgcga gcgccggtaa ccgccgagtc
      121 accgaccgaa gcgttcatct cggcgccgac gacgccgatc ccctcagggc tcaggcctcc
      181 agccggcgcg ggggcgccga cagttcccac gcggcccggc gccaagccgg ctcccccgcc
      241 gcagaagtcg ggctccaggg cctggatggg gatcgccgcg gcagtcgtgg tgctcggcgt
      301 gggggggtat ctggtgttcg ggcgtgggcc caaagccacg aacgacggcg ggcagacggc
      361 ggcgactcag caggcgccgt cgaacccggg tgcttccaat ccggcgacga cgccggcgga
      421 cacgcatgtc gtcgtcaccc cgccggtgac accgccccag caaacggcct cgcagcctcc
      481 ggcgcaggcg acgcacaagc ccgcgccggc gccgcaccgg aatccggcgc cgcagccgaa
      541 gcccgccgcg gccgcgaccg ggttcatcag catcgcgtcc gatcccattg cgatggtcta
      601 catcgacggg caagcggtcg gccagtcgcc ggtcatcaac cacgaagtgc cggtcggctc
      661 gcataccatc cgggtggaac aggtggggtt cgccgcgcag acccagacgg tgcaggtgtc
      721 atcgggtcaa acgtccttca ggaagttcac gctgctccca ggaggctgat ggtgaggcga
      781 cttcgcgtaa tcctctccct ggccgcttcg gccgccgcgg cgcccctcgc ggcccaaggc
      841 acggcgcccc agaatccgac gctccgccgg gcggtccagg cgttcgacaa cctgaatctc
      901 ccgcaggcca tttcgctcgc ccagcaggca ctcggccagc gccttggccc cggcgatcag
      961 gagcaggcgt acactctgct tggatcggcg tacgccaacc aaccggacgg gttcgacaag
     1021 gccatgaatt cgttcaagca ggtgctcctg atcaattcgg agcgcgtgct cgatcccagc
     1081 cggacgccga ccaagacgct ggtcgcgttc aacatggcgc tctccaccat gctcctcgtc
     1141 cgcggactgc aggtggacag ctcgagcttc gtcgtcggcc agggagccgc gccgatccac
     1201 ttcaccgtga ccagcccggc gcatgtgcgg gtaagggcgg tgagcaacag ggcgtcggtg
     1261 accatcgact cgaccatcga gaacggcacc gtgaacatcc ggtggcccgc tacgctggcg
     1321 aacggcgatc cccttccggc gggggattac acggtagtcg tggacgtcac ggccgagcag
     1381 aacagttttt cgtcttcgca gcgcgtgcac ttggccgcgg gagcggtgga cactctcccg
     1441 catctcacca gcgtacccgg atactcgaag cttcccgaca cggaagtccc gccccgcagc
     1501 tggcggcctc tcggcgtcgc gtttctctac gcgggcatcg cgggcgccgg aaccctggcg
     1561 ctggagaata gccagctggg atcggccaac cggcgcgagc tgtccctggt cgcggtcggc
     1621 gtgctggcga ccggcgtcgt gatgatgttg aagaagccct ccccggtgcc ggcgcagggc
     1681 aacattctct acaacaagct gctcgacgag cagctgatcc gccggaatgc cgacatcgcg
     1741 acgcagaacg tcacgctccg ccgccaggta cggctcacgg tcactccaat cccggcgggc
     1801 aaatgaagcg cgccctcatc gcggtcgcac tctgcgcctt tgcggcgccc gcctcggcgc
     1861 aattcgtcaa cgtcggcgtc cagggcgtgt tcgcggacta ccgggagctc ggaccagggc
     1921 tgtcgtacaa tggcggcggc ctgggcggaa tcgccgaact gaactggcac aaattctcgg
     1981 gtgatttcca gatcgccaag atcaattaca acccgtcgtc gggcagcgcg gccacggaca
     2041 agttcacgag cacgcagttc gacgcgcacc tccgctggtt cctggcgaac ggggtgagcc
     2101 ttgaaaccgg ctataccaag cgggatatca gtcccgactt cacgacgcag aatttcaccg
     2161 ccatccgcgc cggcgtccgc gccaactacg gcctcggccc tggcgtgaat ctcctccttc
     2221 ggggcaatta cctcgccgcc gggaagttct cgggcggcgg cacggcgccc ttcgccttgg
     2281 agctgggatt gggtctgtcg gtcggcaagg ccaacaaccg gtaccgcttc acggccgact
     2341 acgagttcca gcgaatcgac cggaagacga cgatatcggg gacggactac aaggtgccga
     2401 ttcagcaggc catggcccgg gttggagcgg cgataggact agggaggtag gcggataggt
     2461 aggcgcccag gcgcttaggc gcctagcggg ttcaaggaac gaggggacag gaagtgttct
     2521 ttcctgtccc ctcttcgttt cctgcaaccc gctgagcgcc tcagcggctg ggcgcctgcc
     2581 tgcactcact tccttctgaa gttccctcag acctgtagta ctttggggat atgagcgact
     2641 cgcggagtgc ggtagttctt ggcgccactg gagcggtcgg gtcgtcggtg gtgcgggcgc
     2701 tgctcgccga cgatgcgtgg aagcgggtga cggtgctcgt ccggcgcccc gacgcattga
     2761 aggacttcaa ggatccgcgc ctcgagcggc acgtggtgga cgtgctcgac gccggacagt
     2821 acgaacgctt cctcgccggg cacggcgccg cgttctgtac catgggcttg ggagaaccgt
     2881 cgaagtgtcc gcgcgaagaa ttcaagaagg tggacctgga cggaacggcc accttcgcgg
     2941 cggcctgtcg ccgccagggc gtcacgcatt ttctgctgct caccgcggtg ggagccaacg
     3001 ccaggtcgtc gatctactac gcgcacatca agggtgaggt ggaagaccgg gtcaggggcc
     3061 tcgggttcgc gcgggcgagt tttttccggc cgtccatgtt gatgacgccg acgaatcgtt
     3121 acggcttcac gcagggcgtg ttcctcaagg ttttcccgtt catcgatccc ctcctggttg
     3181 ggccgttgtc acgcttccgg agcatcacgg tgtctgacct cggcaaggcg atggtccgcc
     3241 acgcggagcg gccgggggcg ggggtggagg tgttggagtg gaaggagttt caggcggtac
     3301 tgcgggggac cggggcgtaa ggcctgacgc catcggtcga gattcttcgt agggtgttcc
     3361 acctccatcc cgaggatgcc atgaaacgcc ctgcgctcgt cattgctctg ttggtgctcg
     3421 catcgattcc gctgcgttcc cagacgcccc cgtccggaga tgaatctgcc gtgcgggccg
     3481 tggtcgaacg ctatctccac ggtctgaaat tcaacgacac gacagcactc cgcgaagcgt
     3541 tctggcctga cgccaagctc tactacgtcc gtggggactc ccttgcccag tggacacaat
     3601 ccagctggta tgcgagcttc gcggggagcg tcgggcacga ggagcaggga gacctgcgca
     3661 tcgcgagtct cgaggtgacg cgggacatcg cgaccgccaa gatcgtggag gagtatccga
     3721 agtcccggta cacggactac gtgtccctgc ttcggattcg cggacggtgg tggatcgtca
     3781 acaaggtcta tacggccgag cggcgcaagg aaggctgagc ctcgagaggg gagcgggatg
     3841 gcgaaagtca ccggaatcgg gggcgtgttc ttcaagagca cgaaggacca caaggccctc
     3901 gcggcgtggt atcagaaaca cctgggcatg cctctcgagt cgtggggcgg cgcgatgctg
     3961 aagtggcctg aggaccgcgc cggggacgac ggagtcaccg tctggcacgt cgcggagccc
     4021 aacactaagt ggttcgcgcc gtcgaccgcg tccttcatga tcaattaccg ggtcgatgac
     4081 atgaccggtc tcctcgcgaa cctccgggct gcaggcatcg agatcatcaa gggcccggac
     4141 accgatgaca acggcacgtt cgcctggatc gtggatccgg acggcaacaa agtggagctg
     4201 tggcagccca aggctcagta ggacacctct atcacgggac cagggcagag ctcacgccgg
     4261 gtgatctgat caagaccggc gccttgtacg tctacctttc tgcgaatctc gacgaagcca
     4321 tatgggatgc ggagatggcg ccgggtgaac gtcccggaag ggtctacgtc gtcgagccaa
     4381 ccggcggggt tggcgaacgg actgatctca agcggccggg ccctccatca atgtcactgc
     4441 agtcccggga gcccttgcga gtcaccggtg aggttaccga ctggctcctg tatcacggca
     4501 ccagggccga cctcaaaccc ggcgacctca tcaagccggg gcacaccccc aacttcggcg
     4561 accggacgcg gacccatgcc tgggtctatt tcgcccggac cctggatgca gccacgtggg
     4621 gtgcggagct ggccagcgga gacgctccgg gacgtatcta cgtcgttgaa cccacaggtt
     4681 tggtggagga cgatcccaac ctgacggaca agaagtttcg gggaaatccg acgaagtcct
     4741 accgttctcg cgatccactg cgggtgacgg gggaggtcaa gaactggaag gggcacccac
     4801 ctgaagccct caaagcgatg aaggaaggtt tgaaacggct caagggtcag ggtgccgaga
     4861 tcattgacta gagggtccgc gcgccagcac aaagccccac gcggcattcg cgtggggctt
     4921 tgtttgcgcc tctccaaagg atgggcggcg cgttagagcg ccgtctggtt cccgaagttg
     4981 acgaagctgg cgctgctggt cgacgagacc gagaagttgt atcccatccc cgacgcgcac
     5041 gattcaggga aggtcgggct cacctgtgtc caaccgggct gcaggacctc gcataccacg
     5101 tacgtgccat cagcgagacc ggagaacgtg tagttcccgt tagcgtcggt gatgacgctt
     5161 gcgttcgttg gtccgaagac ctgcaccgtc cacccggcca agccgctccc cgcggtcacg
     5221 tcacgtacct gacccgtgat cgaggcgcct cctgccggcg gcgggggcgg cgggggaggg
     5281 ggagggggcg ggggtggcgg cgtcaccggc gagcaccccc tgcgcccgtc gtggtgatca
     5341 cccttccgat catgatcgga ccgctgatcg cgatcgaagt ccccatcgtg atcagacact
     5401 cgattgtgat cagagtcctg atcgtggttg aacgacctgg gcgaccggtg ctcgtctgcg
     5461 acgacgcact gcgtcgagtc cttcacggcc gcttggctct gagcggccgc tctcacaaaa
     5521 gacgtggaca gcagcccgag cgccagcaac gggacgcgca tctcatcctc cagaaagggt
     5581 tggacagggc gcgattgacg ctcgcagacg gacggcctgg ggaccgtgtt gggcatcact
     5641 aagggtcgca accgcaagaa tcagatgggg ttggctccgt gctggcccat ttttccgccc
     5701 tcctcccgtc gtctgaccgc tgcgggtgtg aggtggagca ccggagctcc tacgcccaaa
     5761 gcggtggggt ccttcgcgcc tgcggcgctc aggatgacag gcgcgctcgg gtgtttgaaa
     5821 agagagaaga aggaacccct ctatcgtgag ggactctgga tccgaaaccc acgctccgcg
     5881 aggtcctgcc aggccagatt ttgtcgttcg atgagctcga tcttccgtga ccgacgccat
     5941 cccttcagtt ccttctctcg cgcaatggcg tcccgaatgt agacaaactc ctcgaagtgg
     6001 acgagctggg tgacgtgaaa ctgtgaggta aacccacgta cgcgaccccg cttgtgctct
     6061 tcaacgcgtc gcgcgaggtc gttcgtcacg ccgacgtaga gcacgcgggc gcggtttgcc
     6121 atgatgtaga catagtaggt gcgcatccta tcaggatgca ccacctggat gcccggaccg
     6181 tcatcatttt ttcttttctc tatcccaaaa aacgcgcttg tcatcctgag cggagcgcgc
     6241 gatagcgcgc ggagcgaagg accccgctgc tccggccacg aaactcggcc ccgcatggtc
     6301 ttcaagaacc ttgtttgagg gtgcgactga cctgtgccat gggttacctt agtcccactc
     6361 atgaaatact cctatgccca cgtcgactag ccgactgcgc gtgttgtctt tcgggatgtc
     6421 catcgacggc ttcggcgccg gtcccgacca gagcctcaag caccccctcg gcaccacggg
     6481 gcccgagctg atggagtggt tcttccccac ccgcgtgtgg cgggaaa
//
LOCUS       DASUBP010000214         5507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_115851, whole genome shotgun sequence.
ACCESSION   DASUBP010000214 DASUBP010000000
VERSION     DASUBP010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5507
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115851"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1544)
                     /gene="leuS"
                     /locus_tag="VFU75_08295"
     CDS             complement(<1..1544)
                     /gene="leuS"
                     /locus_tag="VFU75_08295"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEU5466876.1"
                     /translation="MSPRDTPEYDPAAVESKWQARWAERHTNEPDLDNPARPNFNLMM
                     FPYPSAEGLHVGNMFAFTGADIYGRFKRLQGYTVFEPIGYDAFGIHSENYAITIGTHP
                     AKLIPKNIAAFRNQLHRLGGMFDWRHELATTDPKYYKWTQWLFLQLYKAGLAYKKRAA
                     VNWCPKDKTVLANEQVINGHCERCNTPVEQRFLEQWFFAITKHAQRLLDNLDRLDWSD
                     STRTLQRNWIGRSEGAELTFETPAGQKITVFTTRPDTVFGATYLVLAPEHPLVDTLTA
                     REQRTAVRGYQREAQSKDVVTRRVGDTTKTGVFLGSYVRNPATGESIPIWIADYVLME
                     YGTGAIMAVPAHDERDHEFATKFNLPIKPVVKGDGPLPFVGDDGVLFNSGDFDGLTCA
                     DARKKIVAWLAGRGAASARVQYRLHDWCISRQRYWGPPIPIIYCDSCGVVPVPEKDLP
                     VILPPIDDFRPDDSGVSPLARHQEWYFVKCPQCGAQARRETDVSDTFLDSAWYFLRYP
                     STEFDDR"
     gene            1608..3713
                     /locus_tag="VFU75_08300"
     CDS             1608..3713
                     /locus_tag="VFU75_08300"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEU5466877.1"
                     /translation="MPSDAQQLRQRFVFAGIGVALALVLVLAVVAGNTTDTFLHHSVE
                     ARSKDVVVRAATIVNQYLHERRREAEALAASPAVVAAARAGLQEANQRSLPKNDINTL
                     ERMFARTTQLGGDPDLQSYLRDYVTHSDFLRLFFTEAHGYTVMGSYKSEDFVQSDESW
                     WTSAFAQGEYVSPPKPDTATGVLSMEYDVAVRPSKTTRPLGVLKAVFPLEQLRVLLAG
                     ASNGDGAYLEIIDGKGALLSAPTRQDLLKTSDVAGAVPKADTAVALTLPTTHGDELIA
                     AVPTNDGAWKVLFRQPVDRAFAGAATARRAIWLGALALLLVTVAALWMLGGWLDRRVT
                     EPVREAGRIAGRVAAGDLSVTVAGTRERTAEVAELISSIQSMVVALRRLVGAIRSAAD
                     ESAAMAAEISASTQEMSASTEEMSATCQDLTKRAADQAQMIRHAAEEAGKILGITTTL
                     AAGATEAARRNADLVGVAKEHRETLDQSTAQLAKLAADVQQGVTEAEALATAGAEIQR
                     FVAQAKAVATQTNTLALNAAIEAARAGPQGRGFAVVADEVRKLASQAAVAAGETADVV
                     RDVLSRIQGTRDRLTRIATTGTAAQAAAHSAAAGLGTVASTAETNDIWSREIANSADA
                     VKALVEEIANQLTTVAKGTDELLASSEEIAASAQEQSASTQEISSSANQLATAADSLT
                     GAVQTFRLLADETRREAAD"
     gene            complement(4164..5165)
                     /locus_tag="VFU75_08305"
     CDS             complement(4164..5165)
                     /locus_tag="VFU75_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466878.1"
                     /translation="MSTAQRFVLVGILLALSGACTDTQPVPLAPSGAKTRPLASVQPL
                     CQLGCVETDPSPNSPGVWLGSGVTPFLCGTENPNGINDSDQDGLSDFCEVQLAVAFAP
                     ELFYSVQDDIRGEPHWVAQAVDGGDDAEILIGFLMSYYKDWGSKALPCSGTPFGQYFD
                     CLGHNGDSEVIYLYVDYDFDTEHWVLKEAQYSQHGTLVSFGRTVFGTTYSYPPQVSYP
                     SHPGAYPRSWVSEGKHANYENQSSCNSGGFLGVDTCVDNSQAARFQVSSTANLGSRSH
                     HTSSQDCMVSNNPSYEYYAGGRVECYWTIKPFRGWVPDSIGGDQSTDYSDILWARGF"
BASE COUNT         1043 a         1784 c         1783 g          897 t
ORIGIN      
        1 cggtcatcga actccgtcga cggatagcgc aggaagtacc aggccgagtc gaggaaggtg
       61 tcggagacgt cggtctcgcg acgcgcctgg gccccgcact gcgggcactt cacgaagtac
      121 cactcctgat ggcgcgcgag cggactcacc cctgaatcat ctggccggaa gtcgtcgatg
      181 ggcgggagaa tgaccgggag atccttctcg ggcaccggga ccacgccgca cgaatcacaa
      241 tagatgatgg ggatcggcgg tccccaatac cgctggcgcg agatgcacca gtcgtgaagg
      301 cggtactgaa cccgcgcgct cgccgccccc cgccccgcca gccacgccac gatcttcttc
      361 cgggcgtcgg cgcaggtgag accatcgaag tcgcccgaat tgaagagcac cccgtcgtcc
      421 ccgacaaacg gcaacggtcc gtcgcccttc accaccggct tgatgggcag gttgaacttc
      481 gtggcgaact catgatcccg ttcgtcgtga gccggcaccg ccatgatggc tccggtgccg
      541 tactccatga ggacgtagtc cgcgatccag atggggatcg attcgccggt cgccggattc
      601 cggacgtagg agccgaggaa cacccccgtc ttggtcgtgt cccccacccg ccgggtcacc
      661 acgtccttcg actgggcctc gcgctgataa ccccgcaccg ccgtccgctg ctcccgggcg
      721 gtgagggtgt cgacgagcgg atgctcgggt gcgagcacga ggtaggtcgc gccgaacacc
      781 gtgtcgggcc gcgtcgtgaa gacggtgatc ttctgccccg ccggcgtctc gaaggtcagc
      841 tccgcaccct cgctccgtcc gatccagttc cgctggagcg tgcgggtcga gtcggaccag
      901 tcgaggcggt cgagattgtc cagcaaccgc tgggcgtgct tcgtgatcgc gaagaaccac
      961 tgctccagga aacgctgctc gacgggagtg ttgcaccgct cgcagtgacc gttgatgacc
     1021 tgctcgttgg cgaggacggt cttgtctttc ggacaccagt tcacggccgc gcgcttcttg
     1081 taggcgaggc cggccttgta gagctggaga aagagccact gcgtccactt gtagtacttg
     1141 ggatcggtgg tcgcgagctc gtgacgccag tcgaacatgc cgccgagccg gtgcagctgg
     1201 ttccggaagg ccgcgatgtt cttcgggatc agcttcgcgg gatgcgtccc gatcgtgatc
     1261 gcgtaattct cggaatgaat cccgaaagcg tcgtagccga tcggctcgaa caccgtgtag
     1321 ccctggagcc gcttgaagcg cccgtagatg tccgcgccag tgaacgcgaa catgttcccc
     1381 acgtgcagcc cttccgccga gggatacggg aacatcatga ggttgaagtt cggccgcgca
     1441 ggattatcga ggtcaggctc attggtgtgg cgctcggccc atcgggcctg ccacttcgac
     1501 tcgaccgctg cggggtcgta ctcaggtgta tcgcgagggg acataagtgc ttgaagctaa
     1561 acgggcttgc ccgcgcgtga aacccaccat actttcgcgg catccccatg ccaagtgacg
     1621 cacagcagtt aagacaacgc ttcgtgttcg ccgggatcgg cgtggcgctg gcgctggtac
     1681 tggtcctggc ggtggtcgcc ggcaacacga ccgacacctt cctccatcat tcggtcgagg
     1741 cccgcagcaa ggacgtggtc gtccgcgccg cgacgatcgt caatcaatac ctgcacgagc
     1801 gccgtcgcga agccgaagcc ctggcagcct ccccagcggt ggtcgccgcg gcccgcgccg
     1861 gattgcagga agccaaccag cggagccttc cgaagaacga catcaacacc ctggagcgga
     1921 tgttcgcccg gaccacgcaa ctgggcggtg accccgacct ccaatcgtac ctgcgtgact
     1981 acgtcacgca ctcggacttc ctgcggctgt tcttcactga agcccacggc tacaccgtga
     2041 tggggtcgta caaatcggaa gacttcgtgc agtcggacga atcctggtgg accagcgcgt
     2101 tcgcgcaggg cgaatacgtc agccccccca agcccgacac ggccaccggt gtcctctcga
     2161 tggaatacga cgtcgcggtg cgccccagca agaccacccg gcccctcggg gtcctgaagg
     2221 ccgtgttccc gctcgagcag ctgcgcgtcc tgctcgccgg agcatcgaat ggcgacggcg
     2281 catacctcga aatcatcgac ggcaagggcg ccctgctttc ggcgccaacc aggcaggacc
     2341 tgctcaagac gtctgacgtc gcaggcgccg tcccgaaggc cgataccgcg gtggcgttga
     2401 ccctgccgac gacccatggc gacgagctga ttgccgccgt gccgaccaac gacggggctt
     2461 ggaaagtcct cttccgccag cccgtggatc gtgcgttcgc gggggccgcg acggcccggc
     2521 gcgccatctg gctcggggca ctcgcgctgc tcctcgtgac cgtcgccgcc ctgtggatgt
     2581 tgggcgggtg gctcgaccgg cgggtcaccg aacccgtccg ggaggcgggg cggatcgcgg
     2641 gccgcgtggc ggcgggcgat ctctcggtga cggtggcggg cacccgcgag cgcaccgcgg
     2701 aggtcgcgga gctcatctcg tcgattcagt cgatggtcgt cgcgctgcgc cgtctcgtcg
     2761 gagccatccg gtcagccgcc gacgagtcgg ccgcgatggc agccgaaatc tccgcctcga
     2821 cgcaggagat gagtgcgtcc acggaagaaa tgagcgccac ctgccaggac ctcaccaagc
     2881 gcgccgccga tcaggcccag atgatccgtc acgccgccga ggaggcgggc aagatcctgg
     2941 ggatcaccac gacgctggcg gcgggcgcga cggaagcggc gcgacgcaat gccgacctcg
     3001 tcggcgtggc gaaggaacac cgggaaacgc tcgatcaaag cacggcccag ctcgcgaagc
     3061 tcgccgccga cgttcagcag ggcgtcaccg aggcggaagc actcgccacg gcgggcgcgg
     3121 agatccagcg cttcgtcgcg caggcgaagg cggtggcgac ccaaacgaac acgctggcgc
     3181 tcaacgccgc gatcgaagcg gcgcgcgcgg gtccgcaggg acgcgggttc gcggtggtgg
     3241 ccgacgaggt ccggaagctc gcctcgcagg cggcggttgc cgcgggagag accgcggacg
     3301 tggtccgtga cgtgctctcc cgcattcagg gcacccggga ccggctcacg cgcatcgcga
     3361 cgaccggcac cgcggcacag gcggccgccc actccgccgc cgcgggcctc ggtacggtcg
     3421 ccagcaccgc ggagaccaac gacatctgga gtcgcgaaat cgcgaactcg gctgacgcag
     3481 tgaaagccct ggtggaagag atcgcgaacc agctgacgac ggtcgcgaag gggactgacg
     3541 agctcttggc ttcgtcggaa gaaatcgcgg cctcggctca ggagcaaagc gcgtcgacgc
     3601 aggagatctc gtcgtcggcg aaccagctgg ccaccgccgc cgattcactc accggcgcgg
     3661 tacagacctt ccggctcctg gccgacgaaa cgcggcggga agcggcggac taatgaacct
     3721 aaagcccgga acatgccgag ccgatgaggt tcagcccacc agggcctatc cccccgaggg
     3781 cgggaccttc gagatactac cgccgatcag accaccgacg atcgatccga ttgtcccgaa
     3841 gaatgctgtg ctcagcaccg tggccgccac gcagttgtgc gagttgcttt ccctgctgca
     3901 ccagactgcg gccccgatcc cccccgccag tccgagcccc acccctccaa ccagaacgcc
     3961 ctcccaacgg tagtcacgag agtccccgaa tgggcgacct tgccgcccga gtcctccccc
     4021 caccctcgac gaggccgtga aagtcggggg ggcggctgag ggaatggggg gaggtagccg
     4081 ctgcccggtg acggggcttg cgaggggtaa gagcccaaag agtagaatgg tcagtcgcct
     4141 catgcagtgc ctcgcatcaa gagtcagaag ccgcgagccc aaagaatgtc gctgtagtcg
     4201 gtcgattggt ctccgccgat gctgtctggc acccaccccc ggaaaggctt gatggtccag
     4261 tagcactcaa cgcgtccgcc agcgtaatac tcgtaggacg ggttgttgct caccatgcaa
     4321 tcctgggagg aggtatggtg ggagcggcta ccgaggttgg cggtcgagct aacctgaaac
     4381 cgggcggctt gagagttgtc cacgcacgta tcaaccccca agaacccgcc gctattgcac
     4441 gatgactggt tctcatagtt ggcgtgcttg ccttcagaaa cccaggaccg agggtaagct
     4501 cccggatggg agggatagga tacttggggg gggtacgagt aggtcgttcc gaataccgtg
     4561 cggccaaaac tcaccaaggt gccatgttgc gaatactgag cttctttgag cacccagtgc
     4621 tcggtatcga aatcgtagtc cacgtacagg tagatgactt ccgaatcacc gttgtgtccc
     4681 aagcaatcaa agtactgccc gaacggcgtc ccactgcagg ggagagcctt gctcccccaa
     4741 tctttgtagt aggacatcaa gaacccaatg agaatctctg cgtcgtcgcc gccgtccaca
     4801 gcttgggcga cccagtgggg ctcgccacgg atgtcgtctt gaacggaata gaagagctct
     4861 ggcgcgaagg caaccgcgag ctggacctcg cagaaatccg agagtccatc ctgatcagag
     4921 tcgttgattc cattcgggtt ctccgtaccg cacaagaatg gagttacacc gctgcccaac
     4981 cagactccgg gtgaattcgg actcgggtcg gtctccacac accccagctg acacagcggc
     5041 tgaaccgagg caagaggtct tgtcttcgcc ccggacgggg caagaggaac gggttgcgtg
     5101 tcggtacatg ctccggacaa tgcaagcaag atgccgacaa gcacgaaccg ctgggcagtt
     5161 gacatggtta catccatttc ggcccgctta ggggacaaga agggggcatg ggcttgatca
     5221 aggttacggt agcgcgccgg atcacgttca aacagggcgg ttttgcggca ccgagggggc
     5281 ggaaacgcga cattcgggtc cagaaggcgt caagcagtgg tgaatctcac cacccgcggt
     5341 cgctttggcc ccgtgtggtt acagcccttc aacctcaagg cagcgcaagc ttccaagaaa
     5401 cgccctaaat cgaaggagcg tgctggacgt tcgaagagta agtaactcta cttcttcaaa
     5461 ttgatcgcgg ttgagagatc gcccttcagc agcgcctcgc ggacggc
//
LOCUS       DASUBP010000215         2285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_7286, whole genome shotgun sequence.
ACCESSION   DASUBP010000215 DASUBP010000000
VERSION     DASUBP010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7286"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            163..1107
                     /locus_tag="VFU75_08310"
     CDS             163..1107
                     /locus_tag="VFU75_08310"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEU5466879.1"
                     /translation="MPLPVVIDTDPGIDDSLALLLALASPELDVRGISVSYGNTVVEN
                     AHRNAVEVVRRAGKRVPLAMGARRPLKRALAVALETHGTTGVGDAKVPPAGSVIDFMR
                     PLEKLLAEQPEPVTLVTMGPITSLAVALKRAPEVVREKVSRHIAMIGNLEARGNTTPF
                     SEFNAWCDPEALAQVLAAELPTDLVTLDVTRELVVTGSEVGALSRVEDPQAQWLSGAL
                     SFYHRFHQQYEGLDGCVVNDVLVIAALIDPSVLTFQPSRLAVSLEDGDERGRTQVDME
                     GWKARVATSVKTEAVRQMLFERVLPWLAEPAGVGGAKR"
     gene            1104..2054
                     /locus_tag="VFU75_08315"
     CDS             1104..2054
                     /locus_tag="VFU75_08315"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HEU5466880.1"
                     /translation="MTQIPRKVAVIGAGDIGCGWSALCVTAGWPVTIFDTNAKAIDRA
                     SEQIPARARALVALDRAPAGQVESGLKELRHARSLLQAVNEADWVIEAISEDLFAKQR
                     LLGSIQDVAGADALVTSSSSGLSPHDIFGRMTSAHRALVVHPLNPPELIPLVEVVPSP
                     RTDPSAIARASDYLRQLGRMPIVLKKAVPGYVVGRIAAAVWRECIDLVLQGVISVEDL
                     DRAVSLGPALGWAAAGPHLTYHLASGDGGVNGFLQHLLGSFEGWWGELATWQKLEPEQ
                     QKELIHAIEKAYSGKVDLLRDARDRRLSAILRGLEQARGT"
BASE COUNT          357 a          768 c          775 g          385 t
ORIGIN      
        1 cggatgaagg cgctgtacca ctaccgcggc ctgccctact cggtgctcct cgaccggcac
       61 gggaggatcg tcaagcgcat cttcggattc ggcgggcctc aggagtttca agagcttgcg
      121 gccaccattg ccaaggaaat cgccgcgccc tagccttacc tcgtgccgct ccccgtagtg
      181 atcgacaccg atcccggaat cgacgacagt ctcgcgctcc tgctcgcgtt ggcctcaccc
      241 gagctggacg tgcggggcat ttcggtgtcg tacggcaaca cggtcgtcga gaacgcgcac
      301 cgcaacgccg tcgaggtcgt gcggcgcgcc ggcaagcgcg tccccctggc gatgggggcg
      361 cgccgtccgc tgaagcgggc gttggccgtc gcgctcgaga cccatgggac aaccggcgtc
      421 ggcgacgcga aggtcccacc cgccggctcg gtgatcgact tcatgcgacc cctcgagaag
      481 ctcctggccg aacagccgga gcccgtgacc ctcgtgacga tgggtccgat tacgtccctc
      541 gcggtggcgc tgaagcgggc ccccgaggtc gtccgcgaga aggtctcccg ccacatcgcg
      601 atgatcggaa acctcgaagc gcgcgggaat acgacgccgt tctccgagtt caatgcgtgg
      661 tgcgatcccg aagccctcgc ccaggtgctg gccgccgagc tgccgactga cctcgtgacc
      721 ctggatgtga cccgcgagct cgtggtcacg gggagtgaag tgggcgcgct ctcccgcgtc
      781 gaggacccgc aggcgcagtg gctctccggt gccctctcct tctatcaccg cttccatcag
      841 cagtatgagg gattggatgg gtgcgtcgtg aacgacgtgc tcgtgatcgc cgcgctcatc
      901 gacccctcgg tgctgacctt ccagccctcg cggctcgcgg tctccctcga ggacggcgac
      961 gaacgcggcc gtacgcaggt ggatatggaa ggatggaaag cgcgggtggc gacgagcgtg
     1021 aagaccgaag cggtgcggca gatgctcttc gagcgcgtcc tgccctggct ggccgagccg
     1081 gccggcgtgg gtggagcgaa gcggtgaccc agatcccgcg gaaagtcgcc gtcatcggcg
     1141 ctggagacat cggctgcggc tggtcggcgc tctgcgtcac cgccggatgg ccggtcacga
     1201 tcttcgacac caatgccaag gccatcgacc gtgccagcga gcagatcccg gcgcgggccc
     1261 gggcgctcgt cgccctcgat cgcgcgccgg cgggccaggt ggagagcggg ctcaaggagc
     1321 tgcggcacgc ccgcagtctg ttgcaggcgg tgaacgaggc cgattgggtc atcgaggcca
     1381 tctcggaaga cctgttcgcc aaacagcgtc tgctcggatc gattcaggac gtcgccggcg
     1441 ccgacgccct ggtgaccagc tcgtcgagtg gcttgtcgcc gcacgacatc ttcggccgga
     1501 tgacctcggc gcaccgcgcg ctcgtggtgc atccgctcaa tccgcctgag ctgattcccc
     1561 tggtcgaagt cgtgccctcg cctcgcaccg acccctccgc catcgcacgc gcgagcgact
     1621 acctgagaca gctgggccgg atgccgatcg tcttgaagaa ggcggtaccg ggctacgtgg
     1681 tcggacgcat cgccgcggcc gtctggcgcg agtgcatcga tctggttctc cagggcgtca
     1741 tcagcgtcga ggacctggac cgcgccgtct ccctcggccc ggcgctgggc tgggccgccg
     1801 ccggcccgca cctgacctat cacctcgctt ccggcgacgg tggcgtgaac gggttcctgc
     1861 agcacctgct cgggtccttc gagggttggt ggggagagct cgccacgtgg cagaagctcg
     1921 agccggagca gcagaaggag ctgatccacg cgatcgagaa agcgtatagc gggaaggtgg
     1981 atttgctgcg ggatgcgagg gaccggaggc tctcggcgat actccggggg ttggagcaag
     2041 ccagggggac gtgacccatt gtcgaggtac cccgggctcg cgcccgggcg tttggagatc
     2101 tcgctcacat ccatcaaccc cgggcttgcg cccggggtta tgtacatccg gttgcgtctt
     2161 cctgatccgt cacatccatc gttacatccg tcaaccccgg gctcgcgccc ggggttatgt
     2221 aaacccaggt gcgtcctcct gccacgctcc gcgtgaatca gatgatggtg gatcaacaag
     2281 gattc
//
LOCUS       DASUBP010000216        10837 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_142914, whole genome shotgun sequence.
ACCESSION   DASUBP010000216 DASUBP010000000
VERSION     DASUBP010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 10837)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10837)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10837
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142914"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1199)
                     /locus_tag="VFU75_08320"
     CDS             complement(<1..1199)
                     /locus_tag="VFU75_08320"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5466881.1"
                     /translation="MTPLLEGVGIALESLRVSKARAALTILGVAIGVMVVMVIGAIIS
                     GFNKGVSDQLARTGPSTFFVVRFFQAGVTVSDGSDEMSPWRRNPPLAVSDARAIEQVP
                     SVAWVAVDEGSNADVSYGNKKQTSVRIDGRSSAWPNVAGGDVSPGRSFTELENQANAT
                     VVVINNKLAELLFDRLDPIGQRIHIQGVPYTVIGVFNPPPQLFGGEPSPEAVIPHESF
                     AKYVQHWKGWMDFLVGPKAGVTTAQAMEDVTEAMRIRRGLRPGTDNNFSIVSQDKILE
                     KLNGITLVFRLVMFALSSVGLMVGGVGVVAIMMISVTERTREIGVRKALGATRREILW
                     QFLVEASTLTVVGGVVGMVGGAVVAALLALFTPVPAHVPLGSVVIALFVAALTGVLFG
                     IVPANKAS"
     gene            complement(1204..2442)
                     /locus_tag="VFU75_08325"
     CDS             complement(1204..2442)
                     /locus_tag="VFU75_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5466882.1"
                     /translation="MPFYEAVVLALQTIRAQKLKSFFSVIGVFIGVTFLIAAVSIVNG
                     MNSYMTDKFAGTIIGVNTFQLRNRPNVNFNVPDSTWRAWRRRPRITFDDADAVTKALT
                     VPVLTAWYSEDQGTVEYGGKVAKSIDLQGAGADYFAIKNLNIVDGRPFTAQEVKAAVP
                     VVVMGWELADKLFAGQEAVGKTVKIQGLPYLVIGVVEKQGNLFGMSLDKFVVAPATSP
                     IQRDVNPPRVVDAVMVKAKSVPEMNQAMDQVEGIMRARHHLRPNQDDNFALETSAAVL
                     DFWSGINKIMLAVGPGIVGVSLVVGGIVIMNIMLMAVAERTREIGVRKSLGARRQDIL
                     RQFLAESATLALAGATLGILSGILLSFILNAATPLPAAVAPWSVGVGVILGAGVGIVA
                     GVYPASRAARLDPITALRQE"
     gene            complement(2442..3170)
                     /locus_tag="VFU75_08330"
     CDS             complement(2442..3170)
                     /locus_tag="VFU75_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5466883.1"
                     /translation="MTHQEFVTGDTPLPDIVILTHKLAREYIMGTETVRALRGVSLQI
                     RRNEYVAVMGPSGSGKSTFMNLVGCLDTPTAGEYWLNGQKVSDLGDDDLARIRNKEIG
                     FVFQTFNLLPRADALHNVELPLVYAGIRAAERKKKASEALARVGLADRMQHRPNELSG
                     GQRQRVAIARALVNDPSILLADEPTGNLDSATSSEIMGVFKELHKQGQTVVMVTHEPD
                     IAANADRVVTLRDGLIHSDQKQAA"
     gene            complement(3167..4444)
                     /locus_tag="VFU75_08335"
     CDS             complement(3167..4444)
                     /locus_tag="VFU75_08335"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5466884.1"
                     /translation="MSKRGKLFLFAGGGILVLGLIVLSASAKRDKGVDVRFDTVSRRD
                     LVAAVTASGKIQPKKKVDISADITGRITEIAVKEGDWVEKGQFLLQIDPTVYEAAVQQ
                     FQAGLASAEAFAVQAQANRDQAQRALTRTKELHTSNPNLVSDEQMEQAQTAFDVADAN
                     YNSSKHQVDQSRANVQQAQDNLRKTHLVAPMAGRVTRLAVETGEVAVPQSFSKDVGLL
                     MTISDLSVIQTLVQVDETDVVRLHLGDSVDVSIDAFPDSTFSGRVTKISDSSVLPAGA
                     SAQTGQNTTAVDYDVEVTLTKPPANVRPDLSATARIITDTRKQALSIPIIALTQRDNK
                     PVVTEGHTPDTASTKKAAQTEGVFVVTNGKATFRPVKVGIAGDQYFEVLDGLKGGETI
                     VAGPYQAIRDLKDGAAVRGTKTPGDSGAVRRAS"
     gene            complement(4464..5936)
                     /locus_tag="VFU75_08340"
     CDS             complement(4464..5936)
                     /locus_tag="VFU75_08340"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU5466885.1"
                     /translation="MRKTLIAVGLFVCPALVGAQSVPASLSLSDAITIARDNNPAYRQ
                     TLATRTASLWNARSAWANLFIPTATASGSIGYTGAGSQNFLATSFSQPVSTASSNYSV
                     NLNWQLSGQTLAAPGEAHANADAVDADIVGARATLINNVTAQYLTALQSRDNAAVAEK
                     QLQSNDEALKLAQAKYAVGSGTLLDVRQAQVARGQAEVALLTAQTDVQVQKLKLFELM
                     GVSAPGDIKTITLSDTFPVAQPHWSLDELLSTAQAENPQLQALESRASAQKWAVRAAT
                     ASYGPSLSLSAGWSGFTQRFNNLDPIITSAQISGSRQWAACNDNNVIRTNAGLSAEDC
                     SGLIFGSAQRQALIDQNSQYPFNFTRQPFQASLRITIPLWSNFQQPISVSQAKSNQEQ
                     ADQAARAQSLALRTLVQNGYLTLQTAYQTIAIQDTNRTAAQEGLKLATDRYRVGSGTF
                     LDVVNAQVADAKAESDYVTAVYNYHKARAALEAAVGKPLN"
     gene            complement(6091..6756)
                     /locus_tag="VFU75_08345"
     CDS             complement(6091..6756)
                     /locus_tag="VFU75_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466886.1"
                     /translation="MIPILLVLALPQVADSGILVIRQDSTLIARESFHTSPGPTSGGW
                     ILDANVRYDRSRPVVQLAPIVQVGSDLAAVKVQFDAGDPGSPLHIIGQVGRHRFTLRY
                     VSRSDERARELPDQGRAVVVDDSVFSLFTPVAWLVRGGAGTVSAIFPRPGVAETWTVR
                     DEGLVSTGGGREGGGADTRLRLVSVESPRLGNIRIWMDAAGRLAKVEIPRLHLSAERQ
                     AGR"
     gene            complement(6753..7475)
                     /locus_tag="VFU75_08350"
     CDS             complement(6753..7475)
                     /locus_tag="VFU75_08350"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEU5466887.1"
                     /translation="MTGGRPLPLRELPPDHVRDILARDPRLLVPVGTCEQHGPHLPLG
                     TDSIIVERLADDLSAAFGILRAPTVEYGVNAITKRPIPGNAGVRRKTLHRLMNDLLTA
                     WEEGGVETFVILTAHGHDPHQEALSTLRTREARIFTVDVFSLDFTELLDDPEGPVHGG
                     ELDTSLLLHLAPDQVRMELAQDFSLAPKALAKYHRGSTSIPTSGPGSVGRPTLATAEK
                     GARVYKMILERIGTRVLGAYAP"
     gene            7365..>10837
                     /locus_tag="VFU75_08355"
     CDS             7365..>10837
                     /locus_tag="VFU75_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466888.1"
                     /translation="MLLARSDGDEQPRIAREDVPHVIGRELSQWQGPAARHRLIAGML
                     AAVGAAEWLRSGGPGWAYADLLLVAFLLLATLWRMPRRWADPGLAVAALIIAAAMLRT
                     VTGTATLSCCWSAAREERLDRASLQLSRELGAAVNDARDLARRAAQAASLPREQAFDR
                     LASAARHGTAGVERGVALVAADGTPIAWAGRHRILPVPDTAELRANISPFYVTLEARR
                     QTAAGAVAVGTVLLDAGPSIPDGDGAVARRFAREFGVDLKFYAPRTAPHDHDVFDYCA
                     KSCDLGDTLFSVKPLPPVQAEAIQTLGREGQRGVAIGLIVLLLFLIMAAPLGSWRWTT
                     LAVAAGTLTRFPMVPMLTESGPSSPTLAQPLLGFASGSAVALGLVSAAVIVAAAALWR
                     RGFPRRWYTLIPAGFLILAAPYLVRYFGRGIRPPAAGVSLLLWLSWEVALAAVAMGLV
                     LLAAALVRGPEEPRRIPWTLAAACAWAILAAVAGLWLWRPYDAWPEWYTFLWLPALAG
                     AIVPARRRATLFTVATVAGTAAALVTWGASIEGRLSLAQRDVAGLGAVQDPRIASALD
                     SLAAITDTIRAPQSAGALYSLWHSSRLASEGYPAVLALWSPEGQLETELRLAALDLPD
                     PLLAALVRRPGAPTLPRVEEIVRIPGLHEVLVAPLLSGEWITVGVGPKTLVIASDRVT
                     RFLRGEGRVTAPYTLTISAPSHTSGATEQFTWSRDGWTVRGERRFDFADGVRHVHALV
                     GLGGAGGLLVRGSLAVLVDLALLVALMGAGGLFVPQPPREGPSPLTQLRQSYRLRLAA
                     ALVAFFVTPVLAFGAWSFADLTQSAAFAGDLLITQTLRDATRSAEQVPFDRPGAPEAI
                     RDLGTRLDAGLWAYRGGALVGTSDPVLADIGVVDPLLVPDVFRRVALRDEIETATTVE
                     AAGRTVRVGYRVVVNGGPTGQVVLAAPQLLDDARVRMEERELALDLALATIAGLAAAA
                     GLAGWAARRLERPVAVLREAAVAVGRGDAPPTFPEVPPIEFAPVMSAFERMVVDIKRS
                     QEALEEARGRTAQVLATVATGVIAVDATLRVTLLNPRAVELLGAPVAPGNELTAATGR
                     EWATVWDDVTRFIALAQDTIAEREVDVGTRRIRVQIALLGPAPDGCVIALDDTTALSR
                     AARVLAWGQMAR"
BASE COUNT         1578 a         3649 c         3729 g         1881 t
ORIGIN      
        1 gaggccttgt tggccgggac gatgccgaac aggacaccgg tgagcgcggc cacgaagagg
       61 gcgatgacca ccgacccgag gggtacgtga gccggtaccg gcgtgaacag ggcaaggagc
      121 gccgctacca cggcgccccc gaccatgccc acgacgccgc cgacgaccgt gagggtcgac
      181 gcctccacga ggaactgcca gaggatttcc cgccgcgtgg ccccgagggc cttgcggacg
      241 ccgatctccc gggtgcgctc ggtcaccgag atcatcatga tcgccaccac gcccacgccg
      301 cccaccatga gcccgaccga cgagagggcg aacatcacca gacgaaacac gagcgtgatc
      361 ccgttcagct tctcgaggat cttgtcctgg gaaacgatgg aaaagttgtt gtcagtcccg
      421 gggcggagcc cacgcctgat gcgcatggcc tccgtgacgt cttccatcgc ctgcgcggtg
      481 gtcacgcccg ccttgggtcc gaccagaaag tccatccagc ccttccagtg ctggacgtac
      541 ttggcgaagg actcgtgggg aatgacggct tcgggagagg gctcgccccc gaagagctgg
      601 gggggcggat tgaaaacccc gatcaccgtg tacgggaccc cctgaatgtg gatccgctgg
      661 ccgatcggat cgagtcggtc gaagagcagc tcggcaagtt tgttgttgat gaccacgacc
      721 gtggcgttgg cctggttctc cagctcggtg aaggaccggc cgggagacac gtcaccgccg
      781 gccacgttgg gccacgccga gctccggccg tcgatgcgca cgctcgtctg tttcttgttg
      841 ccgtagctca cgtccgcgtt ggagccttcg tcgaccgcga cccaggcgac cgaaggcacc
      901 tgctcgatgg cccgggcgtc cgagaccgcg aggggcggat tccggcgcca ggggctcatc
      961 tcgtccgagc catccgagac ggtcacgccg gcctgaaaga agcgaaccac aaagaaggtg
     1021 ctcgggccgg tgcgcgccaa ttgatcgctc acccccttgt tgaagcccga gatgatggcg
     1081 ccgatcacca tcaccaccat cacgccgatc gcaactccga gaatggtgag ggccgcccgt
     1141 gccttggaga cccgcagcga ttccagcgcg atgccgacgc cttcgaggag cggggtcatg
     1201 tggctactcc tgccgcagag cggtgatggg atcgagccgg gcggcgcggc tggcggggta
     1261 gaccccggcg acgatgccca caccggcccc gagaatgaca ccgaccccaa ccgaccacgg
     1321 cgcgacggcc gcggggaggg gcgtcgccgc gttcaggatg aatgagagca ggatcccgga
     1381 cagaattccc agggtcgcgc ccgccagcgc cagcgtggcg gactccgcca gaaactgccg
     1441 caggatgtcc tgccgccggg ctcccagcga cttgcgcacc ccgatttccc gggtccgctc
     1501 ggccaccgcc atcagcatga tgttcatgat cacgatgccc ccgaccacga gggacacgcc
     1561 tacgattccc ggcccgacgg ccaacatgat cttgttgatc ccgctccaga aatcgagcac
     1621 cgcggccgac gtttcgaggg cgaaattgtc gtcctggttg ggtcgcaggt ggtggcgggc
     1681 ccgcatgatg ccctccacct ggtccatggc ctggttcatc tcgggcacgg acttggcctt
     1741 caccatcacg gcgtccacca cccggggcgg attcacgtcc cgctgaatcg gggatgtggc
     1801 gggcgccacc acgaacttgt cgagcgacat gccgaagaga ttgccctgct tctccaccac
     1861 gccgatcacc aagtagggaa gaccctggat cttgacggtc ttgcccacgg cctcctgccc
     1921 ggcaaagagc ttgtcggcca gctcccagcc catgaccacg accgggacgg cggctttcac
     1981 ctcctgggcg gtgaacgggc ggccatccac gatgttcagg ttcttgatgg cgaagtaatc
     2041 ggcgccggcg ccctgaagat cgatgctctt ggcgaccttg cctccgtatt cgaccgtacc
     2101 ctgatcctcg gagtaccagg cggtcaggac cggtaccgtc agggccttgg tcaccgcgtc
     2161 cgcgtcgtcg aacgtgatgc ggggccgccg gcgccaggcc cgccaggtcg aatcggggac
     2221 gttgaaattg acgttggggc ggttgcggag ctggaacgtg ttcacgccga tgatggtccc
     2281 ggcgaacttg tcggtcatgt agctgttcat cccgttcacg atcgacaccg cggcgatcag
     2341 gaacgtgacg ccgatgaaca cgccgatgac cgagaagaag ctcttcagct tctgggcccg
     2401 gatggtctgg agggccagca cgaccgcttc gtagaagggc actacgccgc ctgcttctgg
     2461 tcgctgtgga tcagcccgtc gcgcagggtc acgacccggt ccgcgttggc cgcgatgtcg
     2521 ggctcgtgcg tgaccatcac cacggtctgc ccctgtttat gcagctcttt gaacacgccc
     2581 atgatttcgc tgctggtggc cgagtcgagg tttccggtcg gttcgtcggc cagcaggatg
     2641 ctggggtcgt tcaccagggc gcgcgcgatg gcgacacgct ggcgttgtcc gcccgacagc
     2701 tcgttgggcc ggtgctgcat gcggtccgcc aaccccacgc gggccagcgc ctcgctggcc
     2761 ttcttcttcc gctccgcggc ccggatgccg gcgtatacca gcggcagctc gacgttgtgg
     2821 agcgcgtcgg cccggggcag gaggttgaag gtctggaaga cgaatccgat ttccttgttg
     2881 cggatccgcg ccagatcgtc gtctcccagg tccgagacct tctggccgtt cagccagtac
     2941 tcgccggcgg tgggtgtgtc gaggcacccg accaggttca tgaacgtcga cttgcccgag
     3001 cccgatggtc ccatcaccgc cacgtattcg ttgcggcgga tctggaggct caccccgcgc
     3061 agcgcgcgca ccgtctcggt gcccatgatg tactcacggg cgagcttgtg tgtgagaatg
     3121 acgatgtccg gcagcggcgt atcgccggtc acgaactcct ggtgcgtcac gaagccctcc
     3181 gtacggctcc ggagtcgccc ggcgtcttgg tcccgcgcac ggcggctccg tccttgaggt
     3241 cgcggatggc ctggtagggt cccgcgacga tggtttcgcc acccttcagt ccgtccagca
     3301 cttcgaagta ctgatcccct gcgatgccga ccttcactgg acggaaggtc gccttgccgt
     3361 tcgtcacgac gaaaacgccc tcggtctgcg cggccttctt cgtgctcgcc gtatccggtg
     3421 tgtgcccctc ggtcacgacg ggtttgttgt cccgctgggt gagggcgatg atcgggatcg
     3481 acagggcctg cttccgcgtg tcggtgatga tccgcgccgt cgccgacaga tccggacgca
     3541 cattggcggg gggcttggtc agcgtgactt ccacgtcgta gtccaccgcc gtcgtgttct
     3601 ggccggtctg cgcgctcgcg cccgccggca gcaccgagct gtcggaaatc ttggtcaccc
     3661 ggcccgagaa cgtcgagtca ggaaacgcgt cgatcgagac gtccaccgaa tcgccgaggt
     3721 gcagccggac gacgtcggtc tcgtcgactt gcaccagcgt ctggatgacc gaaagatcgg
     3781 agatggtcat caggaggccg acatccttcg aaaagctctg cggcacggcc acttctcccg
     3841 tttcgacggc cagccgggtg acccggcccg ccatcggggc gaccaggtgc gtcttcctca
     3901 aattgtcctg ggcctgttgc acattggcgc gggactggtc gacctggtgc ttggacgagt
     3961 tgtagttcgc gtccgcgacg tcgaaggcgg tctgggcctg ctccatctgc tcgtcggaca
     4021 ccaggttcgg attcgacgta tgcagttcct tcgtacgcgt cagcgcccgc tgcgcctggt
     4081 cgcggttggc ctgggcctga accgcgaagg cttcagcgga cgcgagcccg gcctggaact
     4141 gctggacggc cgcttcgtag acggtcgggt cgatctggag caggaattgt cccttctcca
     4201 cccagtcgcc ttccttcacg gcgatctccg tgatccggcc ggtgatgtcc gcggagatgt
     4261 cgaccttctt cttgggctgg atcttgccgc tcgcggtcac ggccgcgacc aggtccctgc
     4321 ggctcacggt atcgaagcgg acgtcgacgc ccttgtcacg tttcgcgctc gcggaaagga
     4381 cgatcagccc cagcaccaga atgccgccgc ccgcgaagag aaacagcttg ccacgtttcg
     4441 acatgtcgat tccaatcctt gagttagttc agcggcttgc cgaccgccgc ttcgagcgcg
     4501 gcgcgggcct tgtgatagtt gtagacggcg gtgacgtagt cactttcggc tttggcgtcc
     4561 gccacctgcg cgttcaccac gtcgaggaac gtccccgagc ccacgcggta ccggtcggtg
     4621 gcgagtttca gtccctcctg ggccgccgtc cggttcgtgt cctggatggc aatggtctga
     4681 tacgcggtct gcagcgtcag gtatccgttc tgcaccagcg tgcgcagggc cagggactgc
     4741 gcccgcgcgg cctggtcggc ctgttcctgg ttcgacttcg cctgcgacac ggaaatcggc
     4801 tgctggaagt tgctccagag cgggatcgtg atgcgcaggg atgcctggaa gggctgccgc
     4861 gtgaagttga acggatactg ggaattctga tcgatcagcg cctgcctctg tgcggacccg
     4921 aagatcaggc cgctgcagtc ctcggccgac aggcccgcat tcgttcgaat gacgttgttg
     4981 tcattgcacg ccgcccactg ccgacttccg gagatctggg cgctggtgat gatcgggtcg
     5041 aggttgttga agcgctgcgt gaatcccgac cagcccgccg aaagactcag tgagggacca
     5101 tacgacgccg tcgccgcacg taccgcccac ttctgcgcgc tcgcgcgcga ctcgagggcc
     5161 tggagctgcg ggttctccgc ttgtgcggtc gacagcagct cgtcgaggga ccagtgcggc
     5221 tgcgcgacgg ggaacgtgtc cgagagcgtg atcgtcttga tgtcgccagg cgccgacacg
     5281 cccatcagct cgaacagctt cagcttctgc acctgcacgt cggtctgcgc ggtcaacagc
     5341 gccacctcgg cctggccgcg ggcgacctgc gcctgacgga cgtcgagcag agtgcctgag
     5401 ccgaccgcgt acttcgcctg ggcaagcttg agcgcctcgt cattgctctg cagctgcttt
     5461 tcggcgactg cggcgttgtc acgagactgg agcgcggtga ggtactgtgc cgtgacattg
     5521 ttgatcagcg tcgcgcgcgc gccgacgatg tcggcgtcca ccgcgtcggc gttcgcatgc
     5581 gcctcgccgg gtgccgcgag cgtctggcca ctcaactgcc agttgaggtt gactgagtag
     5641 tttgagctcg ccgtcgacac cggctgcgag aagctggtcg cgaggaaatt ctgggagccg
     5701 gccccggtgt atccgatcga tccggacgcg gtcgccgtgg gaatgaatag attcgcccac
     5761 gcgctgcggg cgttccagag tgacgccgtc cgggtcgcta acgtctggcg gtaggccggg
     5821 ttattgtcgc gcgcgatggt aatcgcatcc gaaagggaca gcgaagcggg aaccgactgg
     5881 gcgccaacaa gtgccgggca gacgaagagc ccgacggcga tcagggtctt acgcatgagg
     5941 gggtccttga ttccaggtcg ttgagtcatc gagtcctacg cgccggactg gcagggggtt
     6001 tcgtccgcca tttctggcgg tccggcctaa gtggcgtccc ctaaaaccta gcgagtcgga
     6061 ccgcgcgagg caggcccgtc ctttaggggt ctagcgtccg gcctggcgct ccgcggacag
     6121 gtggagacgg ggaatttcga ctttggcgag gcggccggcg gcgtccatcc agatcctgat
     6181 gttgccaagg cgcgggctct ccacggagac cagtcgcagg cgggtatcag ctccgccacc
     6241 ctccctccca ccccctgtgg ataccaagcc ctcatccctg acggtccacg tctcggcgac
     6301 gccgggccgc gggaagatgg cggacacggt ccccgcgcct ccgcggacca gccaggcgac
     6361 cggcgtgaag agggagaaca ccgagtcatc caccacgacg gcccggccct ggtcgggcag
     6421 ctcgcgcgcg cgctcgtccg agcgcgagac gtatctcaaa gtgaaccggt gccgcccgac
     6481 ctgtccgatg atgtggaggg gggaacccgg atcgcccgcg tcgaactgca ccttcaccgc
     6541 cgccaggtcg gaaccgacct ggacgatggg cgccaactgc acgaccgggc ggctccggtc
     6601 gtagcggacg ttcgcatcga ggatccaccc cccgctggtc gggccggggc tggtatggaa
     6661 cgattcccgg gcgatcaggg tcgagtcctg acggatgacc aggatcccgg aatcggccac
     6721 ttggggcagg gcgagcacca acaggatggg gatcacggtg cgtaggctcc cagaactcgc
     6781 gtgccgatac gttccagaat catcttatac acccgggccc ccttttccgc ggtggcgagt
     6841 gtcgggcggc cgacggaccc gggcccgctc gtggggatcg aagtgctgcc ccggtggtac
     6901 ttcgccaggg ctttgggagc gagagagaag tcctgcgcca gctccatccg cacctggtcc
     6961 ggcgccaggt gcaggagcag ggaagtgtcc agctcaccgc cgtggaccgg cccttcgggg
     7021 tcgtcgagga gttcggtgaa gtcgagcgag aacacgtcca ccgtgaatat gcgcgcttcg
     7081 cgcgtccgca gggtcgacag cgcctcctgg tgcgggtcgt gcccgtgcgc ggtcaggatc
     7141 acgaacgtct ccacgccgcc ctcttcccac gccgtcagga ggtcgttcat caggcggtgg
     7201 agggtcttcc gccgcacgcc ggcattcccc ggaatgggcc gcttggtgat cgcgttgacg
     7261 ccgtattcga cggtgggggc gcggaggatc ccgaacgccg ccgacaagtc gtccgcgagc
     7321 cgctccacga tgatactgtc ggtccccaac gggaggtgcg gtccatgctg ctcgcacgtt
     7381 ccgacgggga cgagcaaccg cggatcgcgc gcgaggatgt cccgcacgtg atcgggcggg
     7441 agctctctca atggcagggg cctgccgccc gtcaccggct gatcgccggg atgttggccg
     7501 ccgtcggcgc cgccgagtgg ctgcggagcg ggggaccggg atgggcgtac gcggatctcc
     7561 tgctcgtggc gttcctgctc ctcgccacgt tgtggcgaat gccgcggcgc tgggccgatc
     7621 cgggattggc ggtggctgcg ctgatcatcg cggctgcaat gttgcggacc gtgaccggaa
     7681 ccgccaccct gtcctgctgc tggagcgcgg cccgggaaga gcggctcgac cgcgcgtcgc
     7741 tccagctcag tcgcgagctc ggcgcggcgg tgaacgacgc ccgcgacctc gcacgccgcg
     7801 cggcccaggc ggcgtcgttg ccgcgcgaac aggcgttcga ccgcctggcg tccgccgcgc
     7861 ggcacggcac ggccggcgtg gagcgcggtg tggcgctcgt ggcggcggac ggcaccccca
     7921 tcgcgtgggc cggccggcac cggatcctcc cggtgcccga cacggcggaa ttgcgggcga
     7981 acatttcccc attctacgtg acgctggagg cccggcgcca gacggccgcg ggggcggtgg
     8041 cggtcggcac cgttctcctc gacgccgggc cgtcaattcc tgacggcgac ggcgccgtcg
     8101 cccgccgatt cgcgagagag ttcggcgtgg acttgaagtt ctacgcgccg cgcaccgccc
     8161 cgcacgatca cgacgtcttc gactactgcg ccaagagctg cgacctgggc gacacgctct
     8221 tcagcgtgaa acccttgccg ccggtgcagg ccgaggccat ccagaccctc ggtcgggaag
     8281 ggcaacgtgg ggtggccatc ggcctcatcg tgttgctcct cttccttatt atggccgcgc
     8341 cgctggggtc atggcgctgg acaacgttgg cggtggccgc cgggaccctg acgcgtttcc
     8401 ccatggtgcc gatgctcacg gagtcgggac cctcttcgcc cacgctggcg cagccgctgc
     8461 tcggattcgc cagcggatcc gccgtcgcgc tgggattggt gagtgcggcg gtgatcgtgg
     8521 cggccgccgc gctctggcgc cgcgggttcc cccgccgctg gtacacactc atccccgccg
     8581 gatttctgat cctggcggca ccctatctgg tccggtactt cgggcgggga atcagacccc
     8641 cggcggcggg cgtcagtctc ttgctctggc tgtcgtggga agtggcgctc gccgcggtgg
     8701 ccatggggtt ggtgctgttg gcggccgcgt tggtgcgggg accggaggaa ccgcggcgca
     8761 ttccgtggac gctcgcggcc gcctgcgcgt gggctatcct cgccgccgtg gcgggcctct
     8821 ggctctggcg gccgtacgac gcgtggcccg agtggtacac gttcttgtgg ctccccgcgc
     8881 tcgccggggc catcgtgcct gcccgccgcc gagcgacgct cttcacggtg gccacggtcg
     8941 ccggcaccgc cgcggcgctc gtgacctggg gggcctccat tgaagggcgg ctctcactcg
     9001 cgcaacggga cgtcgccggc ctgggcgctg tgcaagaccc caggatcgcc agcgccctgg
     9061 actcgctggc cgcgatcacc gataccatcc gagcgccgca gtccgccggg gcgctctact
     9121 ccctgtggca ttcgtcacgt ctcgcctccg agggctaccc ggcggtcctg gcgctctggt
     9181 ctcccgaggg ccaattggag acggagctcc ggctcgcggc cctcgatctg ccggatccct
     9241 tgctggcggc gctcgtgcgc cgcccgggcg ctcccacgct gcctcgcgtg gaggagatcg
     9301 ttcgaattcc cgggctgcat gaggtcctgg tcgcccctct gctgtcgggc gaatggatca
     9361 ccgtgggggt cggaccgaaa accctggtca tcgcatcgga tcgcgtcacg cgatttctgc
     9421 ggggggaagg tcgcgtcacc gccccgtaca cactcaccat ttcggccccg agtcacacgt
     9481 cgggagccac ggagcagttc acgtggtcgc gggacggctg gacggtgcgg ggagagcggc
     9541 gcttcgattt cgcggatggc gtccggcacg tgcacgcgct ggtgggactg ggcggcgccg
     9601 gcggactcct ggtgcgcggt tcgctcgccg tcctcgtcga tctcgcgctc ctggtcgcgc
     9661 tgatgggggc gggtggtctc ttcgtgccgc agccgccccg cgaggggccc tcgccgctga
     9721 cgcagctccg ccagagctat cgtctccgcc tcgccgcggc gctcgtcgcc ttctttgtga
     9781 cgccggttct cgcgttcggc gcgtggagct ttgccgacct cacgcagagc gcggccttcg
     9841 ccggcgatct cctcatcacg cagacgctcc gcgatgcgac ccggtcagcg gaacaagtcc
     9901 ccttcgaccg tccgggtgcg cctgaagcca ttcgcgacct gggcactcgc ctcgacgcgg
     9961 gactctgggc ctaccggggt ggggccctgg tcggaacgag cgaccccgtg ctcgcggata
    10021 tcggcgtcgt ggatccgttg ctcgttcccg acgtcttccg gcgtgtggcg ttgcgcgacg
    10081 aaatcgaaac ggccaccacc gtggaggcgg ctggccgcac cgttcgggtc ggctatcgcg
    10141 tcgtggtcaa tggcggcccg acgggccagg tggtgctcgc ggctccgcag ctgctggatg
    10201 atgcccgcgt gcggatggag gagcgcgagc tggcgctcga cctggcgctc gcgaccatcg
    10261 ccgggctcgc cgcggccgcg gggctcgccg ggtgggcggc gcgccgcctc gagcggcccg
    10321 tcgcggtgct tcgcgaagcc gcggtggcgg tcggcagggg cgacgcgccg cccacctttc
    10381 cggaggtgcc ccccatcgag ttcgcgccgg tcatgtcggc cttcgaacgc atggtggtgg
    10441 acatcaaacg ctcgcaggag gcgctggagg aggcccgcgg ccgtaccgcc caggtgctcg
    10501 ccaccgtggc gaccggcgtc atcgcggtgg atgccacact gcgggtgacc ctcctcaatc
    10561 cccgggcagt ggagctgctc ggtgcgccgg tggctcccgg aaacgagctg accgccgcga
    10621 cggggaggga gtgggccaca gtctgggatg acgtcacccg cttcatcgct cttgctcagg
    10681 acaccatcgc cgagcgcgaa gtggatgtgg ggacgcgccg gatccgggtc cagatcgcgt
    10741 tgctgggtcc cgcgcccgac ggctgcgtca tcgccctgga cgacacgacg gcgctcagcc
    10801 gcgccgcgcg ggtgctcgcc tgggggcaga tggcgcg
//
LOCUS       DASUBP010000217         3121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1028270, whole genome shotgun sequence.
ACCESSION   DASUBP010000217 DASUBP010000000
VERSION     DASUBP010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3121
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028270"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="VFU75_08360"
     CDS             complement(<1..921)
                     /locus_tag="VFU75_08360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HEU5466889.1"
                     /translation="MAEKIIGIDLGTTNSVVAVMEGGDPVVIPNAEGGRTTPSVVAFT
                     KDGERLVGQVAKRQAVTNPQNTIFSIKRFMGRRMNEVTEETKRVPYKVAAGTNDLASV
                     DIQGKRYTPPEISAMILQKMKQTAEDYLGHKVEKAVITVPAYFNDAQRQATKDAGKIA
                     GLDVVRIINEPTASALAYGLDKKKEEKVAVYDLGGGTYDISVLELAEGVFEVKSTNGD
                     THLGGDDFDQMIIEFLVAEFKRDQGIDLSKDPMALQRLKEAAEKAKMELSSAMQTEIN
                     LPFITADQSGPKHLNLSMTRAKLEQLVDTLV"
     gene            1054..1782
                     /locus_tag="VFU75_08365"
     CDS             1054..1782
                     /locus_tag="VFU75_08365"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEU5466890.1"
                     /translation="MFFPYKDDNPTDLTPTVTVGIIVLNLVVWIVFQGAGSAHALAHS
                     TCVWGLTPGEILRSLPPGVSTPLGQGYACVTTGHPNYLTVFSSMFLHGSWFHILGNMW
                     FLWLFGNNIEDAMGHGRYLVFYLLSGAAAALAQTISAPHSAIPMIGASGAISGVMGAY
                     LVLFPRVKVHTFVFIVIFFTTITVPAYLMLVWWFLLQFLGSLPELGGNSDTGGVAVFA
                     HMGGFVAGLVFVKAFLRGDFRASR"
     gene            complement(1847..2551)
                     /gene="phoU"
                     /locus_tag="VFU75_08370"
     CDS             complement(1847..2551)
                     /gene="phoU"
                     /locus_tag="VFU75_08370"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEU5466891.1"
                     /translation="MSQPPNAPESARPGAGHRHFHDELAALKADLLTMSAEAQDALRE
                     AVDSVLHRDAAVAARVIAGDARVDALELSIEEQVVRLLATQQPMAKDLRLLMAVMKIA
                     NDLERVGDHAVNIAQSAERLLASRSIAPEPEIIEMARQARTMLADALEAFVRGDGQAG
                     RAICRRDDGVDALHRSVFRILLTHMMEDPHIIGAGMDLFLVSRNLERVADLATNIAED
                     VVFLVEGKTIKHHAEE"
     gene            complement(2548..>3121)
                     /locus_tag="VFU75_08375"
     CDS             complement(2548..>3121)
                     /locus_tag="VFU75_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEU5466892.1"
                     /translation="HTGDILLEGVSVFSPGMDPVDLRRQVGMVFQRPNPFPKSIFDNV
                     AYGPRLNGLCREGEMDARVEDALRRAALWDEVKDRLHEPGTGLSGGQQQRLCIARALA
                     NSPEILLLDEPCSALDPIATERIEELLVELKNEVTIVIVTHNMQQASRVADHTGFFYL
                     GHLVEFGETSQVFTMPKDERTEAYITGRFG"
BASE COUNT          479 a         1012 c          991 g          639 t
ORIGIN      
        1 gaccagggtg tccaccagct gctccagctt ggcccgggtc atcgacaggt tcaggtgctt
       61 cggtcccgac tggtccgccg tgatgaaggg gaggttgatc tccgtctgca tcgcggacga
      121 cagctccatc ttggccttct cggcggcttc cttcaggcgc tggagggcca tcgggtcctt
      181 ggagaggtcg atgccctggt cacgcttgaa ctccgccacc aggaattcga tgatcatctg
      241 gtcgaaatcg tcgccaccca ggtgggtgtc gccgtttgtg gacttcactt cgaacacgcc
      301 ttcggcgagc tcgagcaccg agatgtcgta ggtgccgccg cccaagtcgt agaccgcgac
      361 cttctcttct ttcttcttgt cgaggccgta cgccagggcc gacgcggtcg gctcgttgat
      421 gatccggacc acgtcgagac cggcgatctt gccggcatct ttggtggctt gccgctgcgc
      481 gtcgttgaag taggccggga ctgtgatgac ggccttctcg accttgtggc cgaggtagtc
      541 ttcggccgtc tgtttcatct tctggaggat catcgccgag atctcagggg gcgtgtagcg
      601 cttgccctgg atgtcgaccg aggcgaggtc gttggtgccc gcggcgacct tgtacgggac
      661 gcgcttcgtt tcctcggtca cctcgttcat ccggcgaccc atgaaccgct tgatggagaa
      721 gatcgtgttc tgcggattgg tgaccgcctg ccgcttggcc acctgaccga ccagccgctc
      781 tccatccttc gtaaaggcga cgacagacgg agtggtccga ccgccttcgg cgttcggaat
      841 gacgacgggg tctccacctt ccatcacggc caccaccgag ttcgtggtgc ccaggtcgat
      901 gccgatgatc ttctcagcca taaaacggcg actccttacc catggttatt gatgccgaag
      961 cgttccctgg ggtcgctaag agcaaggggt atgccctaga aaagcgccaa aacggcagat
     1021 tgacaagccc caagacttgc atagggttac ggcatgttct tcccctacaa ggacgacaac
     1081 ccgaccgacc ttacccccac cgttacggtg ggcatcatcg tcctgaatct cgtcgtctgg
     1141 atcgtgtttc aaggggccgg gtcagcccac gccctggcgc actccacgtg cgtgtggggg
     1201 ctcacccccg gggaaatcct acgcagtctg ccgccaggag tgtcgacccc cctggggcag
     1261 ggctacgcgt gcgtcacgac gggccatccc aattacctga cggtgttctc ttcgatgttc
     1321 ctgcacgggt cctggttcca catcctcggc aacatgtggt tcctctggct cttcgggaac
     1381 aacatcgagg atgccatggg ccacgggcgt tacctcgtgt tctatctgct ctccggcgcc
     1441 gccgccgccc tcgcccaaac catctccgcc ccccactcgg ccatccccat gatcggcgcc
     1501 tcgggcgcga tctccggcgt gatgggcgcg tacctcgtgc tgtttccgcg cgtgaaggtc
     1561 cacacgttcg tgttcatcgt catcttcttc acgaccatca cggtgcctgc ctatctgatg
     1621 ctggtctggt ggtttctgct ccagttcctc ggcagcttgc cggagctggg cgggaattcg
     1681 gacaccggag gagtcgccgt gttcgcccat atgggggggt tcgtagctgg gctggtgttt
     1741 gtgaaggcgt ttcttcgggg ggatttccga gccagtcgct aaggcgccaa gtcgctaagt
     1801 cggtaagcag tccccttagc gacctagcgt cttggcgact tagcgtctac tcctcagcat
     1861 gatgcttgat cgtcttccct tcgacgagga agaccacgtc ttccgcaatg ttcgtcgcca
     1921 agtctgccac ccgctcgaga ttccggctca ccaggaagag atccatcccc gcgccgatga
     1981 tgtgcgggtc ctccatcatg tgcgtgagca gaatccggaa caccgagcgg tgcagggcgt
     2041 ccacgccgtc atcgcggcgg cagatggccc tgccggcctg accgtcgccg cggacgaagg
     2101 cctcgagcgc gtccgcgagc atggtgcgcg cctggcgggc catctcgatg atctcgggct
     2161 ccggcgcgat ggaccgggac gcgagcagcc gctcggcgga ctgcgcgatg ttgacagcgt
     2221 ggtcccccac ccgttccaga tcgtttgcga tcttcatgac ggccatcagg aggcgcaggt
     2281 ccttcgccat cggctgctgg gtggcgagga gccgtacgac ttgctcttcg atggagagct
     2341 cgagggcgtc gacgcgggca tcgccggcga tgacccgggc ggcgaccgcg gcgtcgcggt
     2401 gcagcaccga gtccaccgct tcgcggaggg cgtcctgggc ttcggcggac atggtgagca
     2461 ggtcggcctt cagggccgcg agctcgtcgt gaaaatgccg gtggccggcg cccggtcggg
     2521 cggactcggg cgcgttgggt ggctggctca tccgaatctc ccggtaatgt aggcctcggt
     2581 tcgctcgtcc ttcggcatcg tgaaaacctg tgaggtttcg ccgaactcga ccagatggcc
     2641 gaggtagaag aatcccgtgt gatccgccac gcgtgacgcc tgctgcatgt tgtgcgtgac
     2701 gatgacgatc gtgacctcgt tcttgagctc caccaggagc tcctcgatcc gctccgtggc
     2761 gatggggtcg agcgccgaac agggctcgtc caggagcagg atctcggggc tgttggcgag
     2821 ggcacgcgcg atgcacagcc gctgctgctg gccccccgac agtccggtgc ccggctcatg
     2881 cagccggtcc ttcacttcgt cccacagggc cgcccgtcgc agggcatcct ccacccgcgc
     2941 gtccatctcg ccttcccggc acaggccgtt cagccgtggg ccgtaggcca cgttgtcgaa
     3001 gatcgacttg gggaacgggt tggggcgctg gaagaccatg cccacctgcc gccgcaagtc
     3061 gaccgggtcc atgccgggcg agaagaccga cacgccctcc agcaggatgt ccccggtgtg
     3121 c
//
LOCUS       DASUBP010000218         9635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_102032, whole genome shotgun sequence.
ACCESSION   DASUBP010000218 DASUBP010000000
VERSION     DASUBP010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9635
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102032"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..416)
                     /locus_tag="VFU75_08380"
     CDS             complement(<1..416)
                     /locus_tag="VFU75_08380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEU5466893.1"
                     /translation="MPFAPDAPFQPAGDQPRAIRELTQGLARGDRYQTLLGVTGSGKT
                     MTMAQVIAAHGKPALVLSHNKTLAAQLYGELKAFFPRNAVEFFISYYDYYQPEAYVPQ
                     TDTYIAKDSSINDDIDRLRLRATSSLIEREDVIVVA"
     gene            complement(427..2607)
                     /locus_tag="VFU75_08385"
     CDS             complement(427..2607)
                     /locus_tag="VFU75_08385"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEU5466894.1"
                     /translation="MTGTVPLLAPELARAVAGQRERLDMDLLARAYDASARAHQGQKR
                     RSGDDFVSHSVAVATILAEQQFDSTTIAAALLHDVVEDSDVTLDDIRRDFGPEIAGLV
                     DGLTKLSSLTFRSTAEEQAENYRKLLLSIAKDARVILIKLADRLHNMRTLEHLSPDQQ
                     QRIALETREIYAPLAHRFGMARVKAELEDLAFRFLEPTDYAELARLIKQTQEERDKVI
                     ERLRGPLLEALTVAGISGAEITGRVKNLWSVHRKMKRRDRPFDEIHDRLAVRVLVNTV
                     AECYHVLGIVHHAWTPLQERIKDYIASPKPNGYQSLHTTVFGPGGRLYEIQIRTREMH
                     RTAEYGIAAHWLFKEGKAADELDQQLGWFRQLLELQQDTHTPEEFLEFLKIDLYQDEI
                     FVFTPRGDVKRLPAGATPLDFAFTVHTEVGQHCQGARINGKISPLHRSLTNGDTVEIL
                     TSPRARPSRDWLAHVRTARARHKIRQWVKQEEHERSVTLGREILDREFRRRRLKPPLA
                     EQLETAAATLSVGTGEMLEASIGRGDVALGQVMKALYPDLPAEATQAPRPTGFGRVMQ
                     RLRLGRGIRIQGVDGLMVRYAQCCQPVPGDAVVGYVTQGRGISIHRADCPNLLTLANE
                     VERRVEIDWQESEGETFVVRLAVGGEDRRGLYADICEAVSGTGTNIRSTELASRDGSV
                     FGALLVEVENQTHLNKVMRAVRRVKGVTEVSRRDSGAQMEGPAV"
     gene            complement(2604..3287)
                     /gene="radC"
                     /locus_tag="VFU75_08390"
     CDS             complement(2604..3287)
                     /gene="radC"
                     /locus_tag="VFU75_08390"
                     /inference="COORDINATES: protein motif:HMM:NF000642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEU5466895.1"
                     /translation="MPLRLSEHLEQDRPRERLWLLGAAALTAQELLAILLGTGVAGQD
                     ALAVAGDILSRVEGSVRRLAGRPPAELAGMPGVGRTKAARILAALELGRRHLVEADPA
                     PDRVRGPADVHRFYSARLRDLPVEEFHVLALGSQSQILGDVLVTRGILNSSLVHPREV
                     FRAAIAEAAAGIIVVHNHPSGDPTPSADDRAVTRQLVDAGRLLDVPVYDHVVIGGGRF
                     VSFAEAGLL"
     gene            complement(3338..6604)
                     /gene="secA"
                     /locus_tag="VFU75_08395"
     CDS             complement(3338..6604)
                     /gene="secA"
                     /locus_tag="VFU75_08395"
                     /inference="COORDINATES: protein motif:HMM:NF009538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEU5466896.1"
                     /translation="MNPYQQTKALVQKVFGTRFSRELKRIQPLVNQIHEHERRLKSLS
                     DAEIQGQTAKFRGILAEQVGPLQAEVDRLKKAKHDCPDVEERLALDRDLAKAEERFKA
                     ALADSLMGLLPEAFATVREACRRLQGASVDVTGHQLLWDMVPYDVQLIGGIVLHQGKI
                     AEMATGEGKTLVATLPLYLNALPQRGAHLVTVNNYLARRDSQWMGHLFRWLGLTVGCI
                     DDTEPQSPDRRAAYGCDITYGTNNEFGFDYLRDNMVFELEQRVQREHHFAIIDEVDSI
                     LIDEARTPLIISGAVGAESDAKYAQFNSQVVQLVRKQAAIVSDLIAAAEPLLKDEKTR
                     YEATTKLYQAQLGMPKNKKLLKLLNEEGVKSLVQRVELDVLADRKSPAKDQRLRHIED
                     DLYFVLDERGHSVHLTEKGVEVMSPEDPQLFVVPDISHEIHQIEHDDELTPAEKVERR
                     RTVEGDYAIKSETLHIIHKLLQAHALYEKDVDYVVQEGQIFIVDEFTGRMMPGRRWSD
                     GLHQAVEAKEGVAVREESQTLATITIQNYFRMYDKLAGMTGTAETEEMEFDQIYRLEV
                     MVIPTNRPVRRMDKHDLIYKTRREKYNAIADEVERQHKRGLPVLVGTVSVEVSETLSR
                     LLKRRGLKHEVLNAKYHEREAEIVAQAGQAGAITIATNMAGRGTDIKLGAGVKQCKVC
                     GLQGREASFAQQVEVPDLQQGEVKRRGCYDDPPCGLVIIGTERHESRRIDRQLRGRSG
                     RQGDPGQSVFYLSLEDDLMRLFGSERIAKLSGGWDENEPISHPWVTSAIGTAQQRVEL
                     QNFQSRKKLLEYDDVMNQQREVIYSLRLFALEGGEELKGEALRMTEEGVAHLADELTG
                     AAKDPIEWDRGLIDTEYLQRFLLSVPGVGDPATVRTRDDLVHAAQEAVRKAFDTKVAY
                     LKELEARAGAPRLADQVLARTMLTVIDEKWKDHLYDLDQLKTGIVYRQWGQKDPLIEY
                     KQEAYQMFVELMHDIRATFTERWFKVQVQFGPPPGQGPAPRTMTGPAGGTPRKAPATV
                     ASKPAADGMIAPPDAPPPAPGRAPGAIVPPSPYAGVGRNDPCPCGSGKKFKKCHGAGA
                     "
     gene            complement(6617..7177)
                     /gene="nadD"
                     /locus_tag="VFU75_08400"
     CDS             complement(6617..7177)
                     /gene="nadD"
                     /locus_tag="VFU75_08400"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEU5466897.1"
                     /translation="MDALFGGSFDPVHLGHLAVAEAAADLLGAAIRFVPAREQPLKRE
                     GHVASASHRAAMLAAAVAGNPRFSVESVELELPPPSYTVATLRALARREPGNRFLLLL
                     GADAAQDLPRWHEAEALPGLADVVVFARPGANVPRTKLISRTITVPAIDISATDIRAR
                     VAAGQSIRYLVPEPVREYIAAHGLYR"
     gene            complement(7183..7929)
                     /gene="bamD"
                     /locus_tag="VFU75_08405"
     CDS             complement(7183..7929)
                     /gene="bamD"
                     /locus_tag="VFU75_08405"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HEU5466898.1"
                     /translation="MKRTVVGAALLALAACGSPTVKTGVTPDAQMTEARALFHRGKFD
                     DALTAFRRLVFELPPGDPTLAEARYYAAECQFQEGLLVEAAHAFRDVADQTPQSPFAP
                     LALLRAGDANLRSWRSPELDPTPGQTALAVYQELVGRYPGTEAALRAQNRVQQLNTAF
                     ATKAYENGMFYFRRRAYDSGIIYFKDVIATYPDTKLVTESLLRLADTYKIIGYSDELK
                     EVCGTLRRFHPAEAASAKSCPPDSTTSAAH"
     gene            complement(7926..8828)
                     /locus_tag="VFU75_08410"
     CDS             complement(7926..8828)
                     /locus_tag="VFU75_08410"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5466899.1"
                     /translation="MAVGAVGAVVACGRSAADHEALGDQEYAAGSYQDALAEYQLGLK
                     ANPENSGLHAKTGAAALHTGNAGLAVVEYRALGVSDRSRVDEAADGLERAARLAQLTQ
                     DHATLASAVGALRALAPGRPLGQYARAALGDALTRGDAGDVLNLLPIAIATTGDPAVA
                     DSLLFLYGQAQARNKACDTASEAFNAVLERKHNRNVEDDARAGLAACDLVTAQNLLAQ
                     GKPVDAEPWFSRATAPEAAADVSRMAWIGLGDVRLAQGDVPGALEAYQSALTGAAPGD
                     SISVMAQGKIAALGRATTPPDSHR"
     gene            complement(8852..>9635)
                     /locus_tag="VFU75_08415"
     CDS             complement(8852..>9635)
                     /locus_tag="VFU75_08415"
                     /inference="COORDINATES: protein motif:HMM:NF024299.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5466900.1"
                     /translation="RGPMDASPEHLVNQARERFQLGDYYGTIHLVEDVVATGRAFADA
                     HHLLGLSYSLVGQYDKALEELDEALELNPRYVEALVHKALVLNQLGREDEADAALRRA
                     GQLGSDTQRGFAGHVAAKLANHHAGLGDAYVEAGGLGEGILQYQAALALGPGFHDLRY
                     KLGRLLLEAGRVLEAREQFEVIVRDRPNFLDASAMLGLACYLSGDGLTARRVWVDLKE
                     RRPEDPRVEAYLGMLDRGEDVSPEEVRAKKSEPVRAKKKKKS"
BASE COUNT         1418 a         3362 c         3155 g         1700 t
ORIGIN      
        1 ctcgccacca cgatcacgtc ctcccgctcg atcaagctcg aggtcgcccg caggcgcagg
       61 cggtcgatgt cgtcgttgat cgacgagtct ttcgcgatgt aggtgtcggt ctgcgggacg
      121 tacgcttcgg gctggtagta gtcgtagtag gaaatgaaga actcgaccgc gttcctgggg
      181 aagaacgcct tcagctcgcc gtacagctgg gccgcgagcg tcttgttgtg tgacagcacc
      241 agggcgggct tgccatgcgc ggcgatcacc tgcgccatcg tcatggtctt gcctgatccg
      301 gtgacaccga ggagggtctg gtaccggtcc ccgcgggcga gaccctgagt gagctcgcgg
      361 atcgcgcgag gctggtcacc ggcgggttgg aacggagcgt cgggcgcgaa gggcattccc
      421 ggaaagttac accgcgggcc cttccatttg agcccccgaa tcccggcgcg aaacctcggt
      481 gacgcctttt acccggcgca cggcgcgcat caccttgttc aggtgggtct gattctccac
      541 ctccaccagc agggcgccga acacggatcc gtcccgggag gccagctcgg tggatcggat
      601 gttcgtcccg gtgccgctca cggcttcgca gatgtccgcg tacaagccac gccggtcttc
      661 accgcccacg gccagccgca cgacgaacgt ctcgccttcc gactcctgcc aatcgatctc
      721 gacccggcgc tcgacttcgt tcgccagcgt caggagattc gggcagtccg cccggtgaat
      781 ggaaatcccc cgcccctggg tgacgtaccc gaccaccgcg tcgccgggca ccggctggca
      841 gcattgcgcg taccgcacca tcagcccgtc tacgccctgg atcctgatgc cgcggccgag
      901 ccggagccgc tgcatgacgc ggccaaagcc ggtgggccgg ggcgcctgag tggcctcagc
      961 ggggaggtcc ggatagaggg ccttcatgac ctgccccagg gcgacgtccc cccgcccgat
     1021 ggacgcctcg agcatttccc cggtccccac ggagagcgtg gcggcggcgg tctcgagctg
     1081 ctcggccagc ggcggcttca gccgccggcg ccggaactcg cggtccagta tctcgcggcc
     1141 cagggtgacg ctccgctcgt gctcttcctg cttcacccac tgccggatct tgtggcgggc
     1201 gcgcgccgtc cgcacatgag ccagccagtc ccgggacgga cgggcccgcg ggctggtgag
     1261 aatctcgacc gtgtcgccgt tggtcagaga ccggtggagc gggctgatct tcccgttgat
     1321 gcgcgcaccc tggcagtgct gccccacttc ggtgtgcacc gtgaacgcga agtcgagggg
     1381 cgtcgcgccc gcgggaaggc gcttcacgtc tccccggggt gtgaagacga agatctcgtc
     1441 ctggtagaga tcgattttca gaaactcgag aaattcctcc ggcgtgtgcg tgtcctgctg
     1501 cagctcgagg agctggcgga accagccgag ctgttgatcg agctcgtcgg ccgccttccc
     1561 ctccttgaag agccagtgcg ccgcaatacc gtactccgcg gtgcggtgca tttcccgggt
     1621 gcggatctgg atctcgtaca gccgtccgcc gggaccgaag acggtggtgt ggagggactg
     1681 gtatccgttg ggcttggggc tcgcgatgta gtccttgatc cgttcctgga gcggggtcca
     1741 cgcgtggtgc acgatcccga gcacgtggta gcactccgcc accgtgttca cgagaacgcg
     1801 cacggcgagc cggtcgtgga tttcgtcgaa gggccggtcg cgccgtttca tcttccggtg
     1861 caccgaccag aggttcttca cccggccggt gatttccgcg ccggagatgc ccgccaccgt
     1921 cagggcctcc agcagcggac cccgcaggcg ctcgatgacc ttgtcgcgct cttcctgcgt
     1981 ctgtttgatc aaccgggcca gctcggcgta gtccgtcggc tcgaggaagc ggaacgcgag
     2041 gtcctcgagc tccgccttca cccgcgccat cccgaaccgg tgcgccagcg gtgcgtagat
     2101 ctcccgcgtc tccagggcga tccgctgctg ctggtccggg gacaggtgct cgagcgtccg
     2161 catgttgtgc aggcggtccg cgagcttgat gaggatgacg cgggcatcct tggcgatgga
     2221 cagcaggagt ttgcggtaat tctccgcctg ctcttcggcg gtggaccgga acgtgagcga
     2281 cgacagtttg gtgagcccgt ccaccaggcc ggcgatctcg gggccgaagt cacgccggat
     2341 atcgtccagg gtgacgtcgg aatcctccac gacgtcgtgg agcagagccg ccgcgatggt
     2401 ggtcgagtcg aactgctgtt ccgcgagaat ggtggcgacc gcgacggagt gcgaaacgaa
     2461 gtcgtccccg gagcggcgtt tctggccctg gtgggcgcgg gcgctcgcgt cgtaggcgcg
     2521 ggccaggagg tccatgtcga gacgctcccg ctgccccgcg acggcgcgcg ccagctcggg
     2581 cgccaggagc ggcaccgtgc cggtcacaac agtcccgcct ccgcaaagct cacgaagcgc
     2641 cctcccccga tcaccacatg gtcgtacacc ggcacgtcga gcagtcgccc ggcgtccacc
     2701 agctgccgcg tcaccgcccg gtcatccgcc gagggcgtcg ggtcccccga agggtggttg
     2761 tgcaccacga tgatcccggc ggccgcttcc gcgatcgcgg cccggaacac ctcccgggga
     2821 tgcaccaacg agctgttgag gatccctcgc gtcaccagca cgtcgcccag aatctggctc
     2881 tgactcccca gcgccagaac gtgaaactcc tccaccggca ggtcccggag cctggcggaa
     2941 tagaagcggt gcacgtcggc cggtccccgt acccggtccg gcgccggatc cgcttccacg
     3001 aggtgccggc gccccagctc cagcgcggcc agaatccgcg ccgccttggt tcgccccacc
     3061 cccggcatcc cggccaactc cgccggaggt cgccccgcga ggcgtctgac cgacccctcc
     3121 acgcgggaca ggatgtctcc ggcgaccgca agcgcgtctt gcccggcgac tcccgttcca
     3181 aggagaatgg ccaggagctc ttgagccgtc aacgcggcgg cacccaacag ccataaacgc
     3241 tcgcgcgggc ggtcctgttc gaggtgctct gaaagccgaa gcggcatgac ccgaacctag
     3301 gtcatgccgc tccggaatcc ggtatcagaa caacgcgtta cgcgcctgct ccgtggcatt
     3361 tcttgaactt tttcccgctg ccacaggggc agggatcgtt ccggcccact cccgcatacg
     3421 gactcggcgg cacgatggcc ccgggggcgc gccccggcgc cggaggcggc gcatcggggg
     3481 gcgcgatcat gccatcggcc gcgggcttcg acgccaccgt cgcgggcgcc ttccgcggag
     3541 tgccgcccgc gggacccgtc atcgtccgtg gtgccggccc ctggcccgga ggcggtccga
     3601 actgcacctg gaccttgaac cagcgctcgg tgaaggtggc ccggatgtca tgcatcagtt
     3661 ccacgaacat ctgatacgct tcctgcttgt actcgatcag cgggtccttc tgaccccact
     3721 gccggtacac gatgccggtc ttgagctgat cgagatcgta gaggtggtcc ttccacttct
     3781 cgtcgatcac ggtcagcatg gtgcgggcga gcacctgatc cgcgagccgg ggcgcgccgg
     3841 ccctggcctc gagttccttc aggtaggcga ccttggtgtc gaacgccttt cgcaccgctt
     3901 cctgtgccgc atggaccagg tcgtcgcggg tccgcaccgt cgccggatcg cccacgcccg
     3961 gcaccgaaag cagaaagcgc tggaggtatt cggtgtcgat cagcccgcgg tcccactcga
     4021 tcgggtcctt cgcggccccg gtgagctcgt cggccagatg ggcgacgcct tcttccgtca
     4081 tccgcagggc ctcgcccttc agctcctccc cgccttccag ggcgaagagc cggagcgagt
     4141 agatgacttc gcgctgctga ttcatcacgt cgtcgtactc cagcagcttc ttgcgggact
     4201 ggaagttctg gagctccacg cgctgctggg ccgtcccgat ggcgctggtg acccaggggt
     4261 gcgaaatcgg ctcgttctcg tcccacccgc cggagagctt ggcgatccgc tccgacccga
     4321 agagccgcat caggtcgtct tccagcgaca gatagaagac gctctgcccc gggtctccct
     4381 gccggcccga gcgtccgcgg agctgccggt cgatgcgccg ggactcgtgg cgctcggtgc
     4441 cgatgatcac caggccgcac ggcgggtcgt cgtagcagcc gcgccgtttc acctcaccct
     4501 gctggagatc cggaacttcg acctgctgcg cgaacgacgc ctcgcggccc tgcaggccgc
     4561 agactttgca ctgcttgacg ccggcgccga gtttgatgtc ggtaccgcgg ccggccatgt
     4621 tggtggcgat ggtgatggcg ccggcctgtc cggcttgggc cacgatttcg gcttcccgct
     4681 cgtggtactt cgcgttcagg acttcgtgct tgagcccgcg ccgcttcagc aggcgggaca
     4741 gcgtttcgga gacctcgaca ctcacggttc cgaccaggac cggcagcccg cgcttgtgct
     4801 gccgttcgac ctcgtccgca atggcgttgt acttctcccg ccgggtcttg tagatgaggt
     4861 cgtgcttgtc catccggcgg acgggccggt tggtcgggat caccatgact tcgagccggt
     4921 agatctgatc gaactccatc tcctccgtct cggcggtccc ggtcatgccg gccagcttgt
     4981 cgtacatccg gaagtagttc tggatggtga tggtcgcgag cgtctgggac tcctcgcgca
     5041 ccgccacccc ttccttcgct tccaccgcct ggtggagccc atccgaccac cgccgtccgg
     5101 gcatcatccg ccccgtgaac tcgtcgacga tgaagatctg cccctcctgc accacgtaat
     5161 ccacgtcctt ctcgtacagg gcgtgcgcct gcaggagctt gtggatgatg tggagggttt
     5221 cgctcttgat ggcgtagtcc ccttcgacgg tgcggcggcg ttccactttc tccgcggggg
     5281 tcagctcgtc gtcgtgctcg atctggtgga tctcgtgaga gatgtcgggc accacgaaga
     5341 gctggggatc ctcgggggac atcacctcca cgcccttttc cgtcaggtgc accgagtggc
     5401 cgcgctcgtc gagcacgaag tacaggtcgt cttcgatgtg ccgcagccgc tggtccttgg
     5461 cgggcgattt ccggtcggcc aggacgtcga gctcgacgcg ctgtaccagg gacttgacgc
     5521 cctcttcgtt caggagcttc aggagcttct tgttcttcgg catgccaagc tgggcctggt
     5581 acagcttggt ggtggcctcg taccgggtct tctcatcctt gagcagcggc tccgccgcgg
     5641 cgatgaggtc ggacacgatc gccgcctgct tcctgaccag ctggacgacc tgactgttga
     5701 actgcgcgta cttggcgtcc gactccgcgc cgaccgcccc cgagatgatg aggggcgtgc
     5761 gcgcttcgtc gatgaggatc gagtccactt cgtcgatgat cgcgaagtgg tgctcccgct
     5821 ggacccgctg ctccagctcg aagaccatgt tgtcgcggag gtagtcgaag ccgaactcgt
     5881 tgttcgtgcc gtaggtgatg tcgcagccgt acgcggcgcg ccggtccggt gattgcggtt
     5941 ccgtgtcgtc gatgcagccg accgtcagac ccagccaccg gaagagatgg cccatccact
     6001 gggagtcgcg ccgcgccagg tagttgttca ccgtgacgag gtgcgccccg cgctgcggca
     6061 acgcgttcag atagagcggg agcgtggcga cgagggtctt accttcgccg gtcgccatct
     6121 cggcgatctt cccctgatgc agcacgatcc cgccgatgag ctgcacgtcg tacggcacca
     6181 tgtcccaaag gagctggtgc cccgtgacgt cgacggaggc gccctgcagg cgccggcacg
     6241 cctcgcgcac cgtcgcgaag gcttcgggca gaagccccat cagggaatcg gcgagggccg
     6301 ccttgaagcg ttcctcggcc ttggccaggt cgcgatccag ggccagacgc tcctcgacgt
     6361 cggggcagtc gtgcttcgcc ttcttgagac ggtcgacctc cgcctggagc ggcccgacct
     6421 gctccgcgag gatgccccgg aacttggcgg tctgcccctg gatctcggcg tccgagagcg
     6481 acttgagccg gcgctcgtgc tcgtgaatct ggttcacgag gggctgaatg cgcttcagct
     6541 cccgcgagaa ccgggtcccg aacaccttct gaacgagcgc cttcgtttgc tgatacggat
     6601 tcatcaaccg ccctgtctac cggtagagtc cgtgtgccgc gatgtactcc cgaacgggtt
     6661 cgggcacgag atatcgaatc gattgtcccg ccgccacgcg ggcgcggatg tccgtcgctg
     6721 aaatgtcgat cgccgggacg gtgatggtcc ggctgatcaa cttggtccgg ggcacgttcg
     6781 cccccgggcg ggcgaacact accacatccg ccaagcccgg aagcgcctcc gcttcatgcc
     6841 agcgcggcag atcctgggcg gcgtccgccc ccaacaacaa caaaaatcgg ttccccggct
     6901 cccgccgggc cagggcccgc agggtcgcca ccgtgtagga tgggggggga agctccaact
     6961 ccacggattc cacggaaaac ctggggtttc ccgccaccgc cgccgccagc atggccgccc
     7021 ggtgggatgc cgaggccacg tgtccctcgc gtttcagggg ctgttcgcgg gccggcacga
     7081 accggatcgc ggcgccgagg aggtccgcgg cggcttccgc gaccgccagg tgcccgagat
     7141 ggacgggatc gaacgagccg ccgaacaggg cgtccacggc gctcagtgcg cggcggacgt
     7201 cgtcgaatcc ggcgggcagc tcttggcgga ggcggcctcg gcgggatgga accggcgcag
     7261 ggtaccgcag acttctttca gctcgtccga atacccgatg atcttgtacg tgtccgccaa
     7321 ccgcagcagc gattccgtga ccagcttcgt gtccggatag gtggcgatga cgtccttgaa
     7381 atagatgatc ccggagtcgt acgcccgccg acggaaatag aacatcccgt tctcgtacgc
     7441 cttggtggcg aacgcggtgt tcagctgctg cacccggttc tgtgcgcgca gggccgcctc
     7501 cgtcccgggg taccggccca ccagctcctg gtagacggcg agcgccgtct gcccgggcgt
     7561 ggggtcgagc tcggggctac gccagctgcg caggttcgcg tccccggcgc gcaacaacgc
     7621 gaggggggca aacggactct gcggcgtctg gtcggcgacg tcgcggaacg catgcgccgc
     7681 ttcgaccagc agtccttcct ggaactgaca ctcggccgcg tagtaacggg cctccgccag
     7741 ggtcgggtca cccggcggca gctcgaagac cagccggcgg aaggcggtca gggcgtcgtc
     7801 gaacttgccg cggtggaaaa gcgcgcgcgc ctccgtcatc tgcgcgtccg gcgtcactcc
     7861 ggtcttcacc gtcggagatc cgcacgccgc gagcgcgagg agcgccgcac caacgaccgt
     7921 tcgtttcatc gatgggaatc cgggggcgtc gtcgcccgtc ccagtgccgc gatcttgcct
     7981 tgtgccatca cgctgatcga atctcccggg gctgcccccg tcaacgccga ttgatacgct
     8041 tcgagcgcgc ccggcacgtc tccctgcgcc aggcgcacgt ctcccagtcc gatccaggcc
     8101 atcctgctca catccgccgc ggcctcgggt gcggtcgcgc ggctgaacca cggctccgcg
     8161 tcgaccggct tcccttgcgc gaggaggttc tgtgcggtga cgaggtcaca ggcggccagg
     8221 cccgcccgcg cgtcatcctc cacgttcctg ttgtgcttcc gttccagcac cgcgttgaat
     8281 gcctcggacg ccgtgtcgca cgccttgttc cgcgcctggg cctggccgta caggaacaac
     8341 agcgagtcgg ccaccgccgg gtcgccggtc gttgcgatcg cgatcggcaa cagattgagc
     8401 acgtcccccg catcccctcg ggtcagtgcg tctcccaacg cggcccgcgc atactgcccc
     8461 aggggccggc ccggcgcgag cgcacgcaat gccccgacgg cgctcgcgag ggtcgcatgg
     8521 tcctgggtca gttgcgccag ccgcgccgcc cgctcgagtc catccgcggc ttcgtccacc
     8581 cgggagcggt cgctcacgcc caaggcccgg tactcgacca ccgcgagccc ggcgttcccg
     8641 gtatggagcg cggcggctcc cgtcttggcg tgcagcccgg agttctcggg attggccttg
     8701 aggccgagct gatactcggc cagcgcatcc tggtacgagc cggccgcgta ctcctgatcg
     8761 cccagtgcct cgtggtccgc ggcgctgcgc ccgcaggcca cgacggcccc gacggccccg
     8821 acggccacca gaacaaggac tccaaagcgc ttcacgattt ctttttcttc ttggcccgga
     8881 ctggttcgga tttcttggct cttacttcct ctggggacac gtcttcgccg cggtcgagca
     8941 tgccgaggta tgcctcgacc cgggggtctt cgggacgccg ttccttcagg tccacccaga
     9001 cccggcgcgc ggtgagtccg tccccggaca agtagcacgc cagcccgagc atggccgagg
     9061 cgtcgagaaa gttcgggcgg tcccgtacga tcacttcgaa ctgctcccgg gcctcgagca
     9121 cgcgccctgc ctcgagcagc aaacgcccga gcttatacct caaatcatgg aagccgggtc
     9181 cgagggccag cgccgcctgg tactgaagga tcccctcccc caggccacct gcctcgacgt
     9241 aggcatcccc cagaccggcg tggtggttcg ccagcttggc ggctacgtgc ccggcaaacc
     9301 cccgctgggt atcgcttccc agctgcccgg cgcgccgcaa ggcggcgtcc gcctcatcct
     9361 cccggcccaa ctgattgagg accagtgcct tgtgcaccag cgcttcgacg tacctggggt
     9421 tcagctccag tgcttcatcg agctcctcga gcgccttgtc gtactggccc accagggagt
     9481 acgacaggcc caggaggtgg tgggcgtcgg cgaacgcccg gcccgtggcg accacatcct
     9541 cgacgaggtg aatggtccca tagtagtcgc cgagctggaa gcgctcccgc gcctgattga
     9601 ccagatgctc aggactggcg tccataggcc ccctc
//
LOCUS       DASUBP010000219         3391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_919859, whole genome shotgun sequence.
ACCESSION   DASUBP010000219 DASUBP010000000
VERSION     DASUBP010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3391
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_919859"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="VFU75_08420"
     CDS             complement(<1..1196)
                     /locus_tag="VFU75_08420"
                     /inference="COORDINATES: protein motif:HMM:NF014758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HEU5466901.1"
                     /translation="MAAAKELRVVGKAHRKVDAVGKVTGALRYADDLSLPRMLYGKLL
                     RSPHPHARIVSIDTSKALAVEGVKAVLTGKDTPIPFGILPVSQDEHALCPDHVRFVGD
                     PVAAVAATSEETATAALDLIDVKYELLPSIASPEDAVTTPEPRIHDYGDSGNLHKLIN
                     LEFGDVEEGFEEADHVREDMFFFEGNTHLPMEQHACLAEWSADGKVTLWSSTQTPHYV
                     HRALAKVLELPPARVRVIACPNGGGFGGKSDPFNHEIVACKLAMLTGRPVKITLTREE
                     VFYCHRGRHPVLMWVKTGVKKDGAITGMHFKSLLDGGGYGSYGVASTYYTGALQTVTY
                     QVQRYKFQGARAFTNKPPCGPKRGHGTPQPRFALEVHLDKIADDLKLDPAELRLKHLV
                     PAGSLTA"
     gene            complement(1183..1704)
                     /locus_tag="VFU75_08425"
     CDS             complement(1183..1704)
                     /locus_tag="VFU75_08425"
                     /inference="COORDINATES: protein motif:HMM:NF013923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HEU5466902.1"
                     /translation="MPELNPQISLTVNGEPAQVAFAPHKTLLEVLREDLGLTGTKHGC
                     ELGECGTCAVLVDGVPVLSCLVLGLTCAGKSVKTVEGMAQGARLHPLQEAFADLGAAQ
                     CGYCTPAILLVAEALIAANPRPSRDQIKEVLAGNLCRCTGYIKIYEAIELAAARMRGE
                     KAEPSQEAMYGRR"
     gene            complement(1719..3173)
                     /locus_tag="VFU75_08430"
     CDS             complement(1719..3173)
                     /locus_tag="VFU75_08430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5466903.1"
                     /translation="MTHLIPAVEALILVYFVALNSFYGLLLLMSIPEVWEQTRLAEDE
                     DLQKLMLSDALPPLTIMVPAYNEGETIQASVTAMLTLEYRQYEVVVVNDGSKDDTMQR
                     LRDAFDLYEVPRIYPETIITKPIRAVYRSRSRTRLTVIDKENGGKADGLNAAINASRY
                     PLVIAVDADTLIEPDALLRLTRPFLLGGNVAAVGGTIRVANDCTVQHGRVLDPRVPRS
                     FLPGVQVVEYLRAFLFGRLGWNRLGGNLIISGAFGLFRREYLLTIGGYLTNSVVEDMD
                     VVVRLHRHLRERKIPYVMPFIPDPVAWTEVPSSLAVLGRQRERWHRGLIASLWTYRDM
                     LFNPRYGQVGLVAVPFYVFGEMLAPVVEVIGYVIAVIAVATGRVDPVFAILFFLFAWG
                     YGLCLSIWGVVLEETSFRKYRRSGDLGRLLVYALLENFGYRQCTVWWRLKAFWAAWRR
                     RKTWGEMTRTGFGGPPRQSRLYAQSPSGMWQKVE"
     gene            complement(3170..>3391)
                     /locus_tag="VFU75_08435"
     CDS             complement(3170..>3391)
                     /locus_tag="VFU75_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU5466904.1"
                     /translation="GLLAPPAALPGLRKALRDPEWWVRLRAALALTRFGGAGRDALVQ
                     EERGPNAGARNTTELVLGLSPQALAEYAT"
BASE COUNT          566 a         1106 c         1141 g          578 t
ORIGIN      
        1 gcggtgaggg atccggcggg taccaggtgc ttcaaccgga gttcggcggg atcgagcttg
       61 aggtcgtccg cgatcttgtc gaggtgtacc tcgagcgcga agcgcggctg cggggtgccg
      121 tggccccgct tgggcccgca ggggggcttg ttggtgaagg cgcgggctcc ctggaacttg
      181 taccgctgca cctgatacgt gacggtctgc agggcacccg tgtagtaggt gctcgccacg
      241 ccgtaggagc cgtacccgcc gccatcgagc agggacttga agtgcatgcc cgtgatggcg
      301 ccatcctttt ttactcccgt cttgacccac atgaggactg gatgacggcc gcggtggcag
      361 tagaacacct cttcgcgggt cagcgtgatc ttgaccggcc gccccgtgag catcgcgagc
      421 ttgcaggcga cgatctcatg attgaaggga tcgctcttgc cgccgaagcc gcccccgttg
      481 gggcaggcga tgacgcggac tcgtgccggg ggcagctcca ggactttcgc gagcgcgcga
      541 tgcacgtagt gcggcgtctg tgtcgagctc cacagcgtca ccttcccatc cgcggaccat
      601 tcggcgaggc aggcgtgctg ctccatcggg aggtgcgtgt ttccctcgaa gaaaaacatg
      661 tcttcgcgga cgtgatccgc ttcttcgaac ccctcctcga catccccgaa ctcgagattg
      721 atgagcttgt ggagattgcc ggagtcgccg tagtcgtgaa tgcgtggttc cggtgtcgtc
      781 accgcgtctt ccggggacgc aatgctgggc aggagctcgt atttcacgtc gatcaggtcg
      841 agtgccgcgg tcgccgtttc ttcactcgtc gccgcgacgg ccgccaccgg gtcgcccacg
      901 aagcggacgt gatcaggaca gagcgcgtgc tcatcctggc tcacgggcag aatgccgaac
      961 gggatcggtg tgtctttccc cgtcagcacg gccttgacgc cttccactgc cagggccttc
     1021 gacgtgtcga tggagacgat gcgcgcgtgg ggatggggag agcggagcaa cttgccgtag
     1081 agcattcgcg gcaaactcag gtcatcggcg tatcgaagcg cgccggtgac cttgccgacc
     1141 gcgtccacct tgcggtgggc cttgccgacg actcgcagct ccttagcggc ggccatacat
     1201 cgcttcctgc gacggctcgg ccttctcgcc ccgcatgcgc gcggccgcga gctcgatggc
     1261 ctcgtagatc ttgatgtagc cggtgcaccg gcagaggttg cccgccagga cctccttgat
     1321 ctgatcccgg ctggggcggg gattcgcggc gatcaacgcc tcggcgacga gcaggatggc
     1381 cggggtgcag tacccgcact gggccgctcc cagatcggcg aatgcctcct gcagcggatg
     1441 gagcctggct ccctgggcca tgccctcgac agtcttcacc gattttccgg cgcaggtgag
     1501 ccccaggacg aggcacgaga ggacggggac tccatccacg agcacggcgc aggtgccgca
     1561 ctcaccgagc tcacagccgt gcttggtccc ggtcagcccc aggtcctccc gaaggacttc
     1621 caggagcgtc ttgtgggggg cgaatgcgac ctgtgccggt tcgccgttga cggtgaggga
     1681 gatctgcggg ttcagctcag gcatccccta atctacggct actccacctt ctgccacatc
     1741 ccgctcgggg actgtgcgta caggcgggac tggcgcgggg gaccgccgaa cccggtgcgc
     1801 gtcatctccc cccaggtctt ccggcgccgc cacgccgccc agaacgcctt gagccgccac
     1861 cagaccgtgc actgccggta gccgaagttc tcgaggagtg cgtagacgag cagtcgtccc
     1921 aggtctcccg accggcggta cttccggaat gacgtctctt ccaggaccac tccccagatc
     1981 gacaggcaga gtccgtagcc ccaggcgaag aggaagaaca ggatcgcgaa taccgggtcc
     2041 acgcggccag tggcgacggc gatcacggcg atcacgtagc cgatcacttc caccaccggc
     2101 gcgagcatct cgccgaatac gtagaagggt accgcgacga gtccgacctg cccgtaccgc
     2161 ggattgaaca gcatgtcgcg gtaggtccag agggaggcga tcaggccccg atgccagcgc
     2221 tcgcgttgcc ggcccagcac cgccaggctg gacggcactt cggtccaggc cacggggtcg
     2281 ggaatgaacg gcatgacgta ggggatcttc ctttcccgga ggtgccggtg caggcgcacc
     2341 acgacgtcca tgtcttccac gacgctgttc gtgaggtacc cgccgatggt gagcagatat
     2401 tcccgccgga atagcccgaa cgcgcccgag atgatcagat tgccgcccag gcggttccag
     2461 cccaggcgcc cgaagaggaa ggcacggagg tactcgacga cctgcacgcc cgggagaaac
     2521 gagcgcggca cccgagggtc gagcacccgg ccatgctgca cggtgcagtc gttggccacg
     2581 cggatggtgc cgccgaccgc cgcgacgttc ccgccgagca ggaaggggcg ggtgaggcga
     2641 agcagggcgt cgggctcgat gagggtgtcc gcatcgacgg cgatcaccag cgggtagcgt
     2701 gacgcgttga tggcggcgtt cagcccgtcc gccttcccgc cattttcctt gtcgatcacc
     2761 gtgagccgcg tgcgggaccg cgagcggtac accgcacgga tcggcttggt aatgatggtc
     2821 tcgggataga tccggggcac ctcgtacaga tcgaaggcgt cccgcaagcg ctgcatggta
     2881 tcgtccttcg aaccatcgtt cacgaccacg acttcgtact gccggtactc gagggtgagc
     2941 atggccgtga cgctcgcctg aatcgtctct ccctcgttgt acgccgggac catgatcgtg
     3001 agaggcggaa gcgcgtcaga cagcatcagt ttttgcagat cctcgtcttc ggccagccgc
     3061 gtttgctccc agacctccgg gatggacatg agcagaagca gcccgtagaa cgagttcagg
     3121 gcgacgaaat agaccagaat gagggcttca accgcgggga tcaggtgagt cacgtcgcgt
     3181 actccgccag cgcctgcggc gagagtccga gcacgagctc ggtggtgttg cgggctccgg
     3241 cgtttggccc ccgctcctcc tgcaccagcg cgtcgcgtcc cgcgccgccg aagcgggtga
     3301 gcgcgagcgc cgcccgcaac cggacccacc actcaggatc ccgcagcgcc ttccgcagtc
     3361 cggggagggc ggccggcggg gcgagcaggc c
//
LOCUS       DASUBP010000220        10527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_552072, whole genome shotgun sequence.
ACCESSION   DASUBP010000220 DASUBP010000000
VERSION     DASUBP010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 10527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10527
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_552072"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="VFU75_08440"
     CDS             complement(<1..1013)
                     /locus_tag="VFU75_08440"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010478.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEU5466905.1"
                     /translation="MSVPEFISERVPLAPLTTLGLGGPAQYLAECRTTDEIVEALRWA
                     AAHRLGVTILGGGSNVVLPDEGLRGLVLHVTTNGLEFRESNGEVEAEAGVSWDTLVQG
                     AVARNWAGVECLSGIPGTVGGTPIQNVGAYGQEIASTLRSVTCLDRTTLAVHTFGPGE
                     CEFGYRTSRFKERDRSRYVVLAVSLRLTPEGAPQIAYADLVKAVRPSATLREVRDAVL
                     AIRRSKGMLIESAASHRSAGSFFTNPVIDAAAFEALETRCRESGLPPMPRYPAGPGRV
                     KLSAAWLVERAGFTRGFRKGGVGVSPQHTLALVNLGGTTAELLSFAGDIIEAVEARFG
                     VRLA"
     gene            complement(1062..2528)
                     /locus_tag="VFU75_08445"
     CDS             complement(1062..2528)
                     /locus_tag="VFU75_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466906.1"
                     /translation="MSSVRLIPAVLAVAGLVACEQPRDPMQPATARGTALSVAATGNQ
                     APMPDPSGIVATYSTSGGLDLRGPFFQSLGTNGRSCASCHVIGNAMGLSADYARQLFA
                     ASAGTDPLFAPVDGANCPNTPPGNDPDAHSLLIGNGLIRVGITEQGVTQYTLSVVHDP
                     YNCAAHTDSTGTTVSVYRRPLPATNLGFLTAVMFDGRETVMPLNNPATFAANLRADLS
                     HQAMDATLGHAQAAVAPTSAQVAGIVDFEMSLYSAQRADNAAGELNASGANGGPGALA
                     GQPFYPGINDPLGGNPTGAAFDPASMSIFQRWLSLTNSGKQTPARQSIARGEQIFGTH
                     ALTITNVRGLNDALGLPAITGTCTTCHNAPNVGDHSLPLPLDIGTSHAVAYETDPHIL
                     AALQQLSTPDLPIYEVACPSGTSFTSDPGKALLTGKCADLNRIKGPILRGLAARAPYF
                     HNGAAASLEQVVAFYNARFQMNLTAQEQSDLVAFLKSL"
     gene            complement(2574..5291)
                     /locus_tag="VFU75_08450"
     CDS             complement(2574..5291)
                     /locus_tag="VFU75_08450"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5466907.1"
                     /translation="MTTPLDPLDWQALNALLDAALELEPEARAAWLAERRRERPDLVD
                     HLEQLLAREARADAEGFLSPAEAPPMAELLASPVTRELGPWRLERPIGQGGMGTVWLA
                     SRADGRFKATAAIKFLALALSGPEGESRFRAEGHVLARLTHPNIARLLDAGVSPGGQP
                     YLVLEHVDGQAIDQWCDEHRLNVAGRVALFQQVLAAVAHAHANLIVHRDLKASNILVA
                     KDGTVKLLDFGIAKMLEGAPEDDTVTRTRMLTFDAAAPEQIRGEPVSTATDVYALGIL
                     LYQLLAGRHPTNEDCPTPAARARAIVEVEPARLSRSVTPQAAETRGAGIDRLRAQYAG
                     DLDNIVAKALDKDPGRRYQTVAALADDLTRYLQHVPVTARRATIVYRTAKFVRRNRGT
                     VVAGALVWLALIGAAVVTALQARAARVQRDEARYQSQRADAQVEFQTLLLSEVGDKPM
                     TMGELLDRGRQLLLRQGGDPRFLSSLLVDLSDRYGEIGARPVRDSLLNVADSIATSLR
                     SPALMAEVRCDRVDQLRTEGRYDEARKVLSGADSLREGADDPTVSVGCLETRGNLLME
                     TGHFDSAGLAFSEAVAIKTRIGQTRDAAFFSLLSGLAGAQDEGGNPRAALATFHRALT
                     GMDSSGRGGMMERVIMEHDNAYALARLGELAEAERMYHDVLMRAARADAAGRIDWQPL
                     IHYAETALIEAHGDSAAKYFGVIVDRAVAQKDSYWLGRGLYGLARAQIATGDLQKARA
                     TAARFRTVLKAFPRVQNTDDVVPDTTTLEGMFAQAAGQARVAQQAYLTALHRSGWFEG
                     KRRIRMRPVVMAAGDLAIQLGWTDSAFFYARMLDSTSSWDSLTYRRSGWIGAAGLLEA
                     RARLASGDTAQARIAATNARRALTNGLGPDHPLTRGADSLLSRLSGLKP"
     gene            complement(5288..5794)
                     /locus_tag="VFU75_08455"
     CDS             complement(5288..5794)
                     /locus_tag="VFU75_08455"
                     /inference="COORDINATES: protein motif:HMM:TIGR02999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type sigma factor"
                     /protein_id="HEU5466908.1"
                     /translation="MSGTSDQLFAALYHELHALAQRRLRGLPYGSTLSTTTLLHEAYL
                     SLADNDQLRFPDRSRFLSYASRAMRGLIIDYARRRSAAKRGGEFQLLPLDGSEEGPGV
                     LPSELDALGLALDELATLDPPLAELVDLHFFCGLSFTEIAGMRGVSERTIQRDWRKAR
                     VILHHQLR"
     gene            complement(5831..6895)
                     /locus_tag="VFU75_08460"
     CDS             complement(5831..6895)
                     /locus_tag="VFU75_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466909.1"
                     /translation="MTGFLRNLPIAGLILAACYAGPEPAVMPEPGQLARIRHLRIEAP
                     ASACPGKAFAATYDGQLDDGSWLPHRLPPELVRLESPDATPLVTGDWAPDPNPINSLN
                     SGFQLIATLRTDTTVADTLVIPPEYECLARGFRFEGAPGEPGGRGAVGPAVSVSIGVV
                     RTRYYKALIVAGIRVLDAPPFYVVADASKVPPSGWISVVSVGGQGGIGAPGVPGRNGD
                     RGGDGCPGDPGGPGQGGGPGGNGGPGGRGGQVTIVGPDDWPLFASLIDARSEGGRGGP
                     GGQGGPGGEGGMGGRHPPVTRLSCADGPIGPSGRRGSAGRDGPRGAPGPHPQIQAQPA
                     KEVFGRDAPLALQVLVSATP"
     gene            complement(6961..7968)
                     /locus_tag="VFU75_08465"
     CDS             complement(6961..7968)
                     /locus_tag="VFU75_08465"
                     /inference="COORDINATES: protein motif:HMM:NF040149.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5700 domain-containing putative Zn-dependent
                     protease"
                     /protein_id="HEU5466910.1"
                     /translation="MVHAVTLALLMQSAAPRIPAVDVRLVTDEPDAVVAILRLRQAGR
                     APTDADWLRLFSSAGYRALARRETSFKRPFSEDRFRAFVLSDSLLARAPQIIATLDAW
                     RHIDVHDAAALALAYLPPGTPLHSTVFFEIKPQGNSFVFDLDGERAIFLYVDPSKSGP
                     QTENTMAHELHHVGITAACRDTGAVRDSAVWRTEQWMTGFGEGYAMLAAAGGPDVDPH
                     ALDDSATRARWNHDAGNFNADLARVQAFFFDILDHRIPTDSIAEHGMSFFGIQGPWYT
                     VGWRMAVVVEKTYGRPRLISEMCAPARFLATYDQAVRETHRTAQLATWSDSLFEKLRP
                     H"
     gene            complement(8015..8854)
                     /locus_tag="VFU75_08470"
     CDS             complement(8015..8854)
                     /locus_tag="VFU75_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466911.1"
                     /translation="MIPMILGVLGVALMQAQNQSATPVIVMDGILSAEHDDSPAFTPD
                     GGTFYFDRTIGHDKSIMASHWQAGHWSAPVTVPFSGRWLDQDPAVSPDGSYLVFSSNR
                     PVPGGDSVVFEQDGKKYRGANLWRVDRHGDGWGTPVWLGSGVNGSNLVVAPSVAANGA
                     VYFIQRLGGAMHIYRSEYRNGAYLPATRVALGDSAQPTHDPAIAPDESFLVFDYGKGD
                     GNLGRLSIAFREGGHWSAPQDLGDEINKDAPWGAHLSPDHRTVYVTGNTHIWSLSLAP
                     TKP"
     gene            complement(8956..>10527)
                     /locus_tag="VFU75_08475"
     CDS             complement(8956..>10527)
                     /locus_tag="VFU75_08475"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5466912.1"
                     /translation="TGIVLLIACANIANLLLARGAGRAMEMGLRLALGASRARLIAQL
                     LVESLALACLGGVASLLVAKWTLTGVASLIPAATSGGLAFTLQPSVLLFSAVLTVTTG
                     IAFGLFPAWHSTRGDLVTTIRANAGHISGARAAARFRSSLVTLQISLATALLIAAGLF
                     MKSLVNVARADLGIHIDSVVTFQLSPEQTGYQDPRTAAYYARVEEAVAGIPGVTSLTT
                     SNVPIFAGRNRGRPVYTAGFPSGPDVDRGASYNLVGTNYFSTLGVHLLAGREFTDADR
                     RGAPRVGVVNQAFVKKFGLTSGAVGKYFSDPPDTANIQIVGVVPDVKYSEVKATVPAV
                     FYLPWRQRPDIPSMSFYLRTARPEAVMRSIPGVVRKIDPGIPVEGLRTLAQQVRDNVY
                     MDRMISILSTAFAVLATLLAAVGLYGILAYSVAQRTRELGVRMALGADAGRVRMMVLR
                     QVGGMMVIGGVIGLASAVVFGRAASSLLFGLNGTDPGVFALSLMVLTLVALAAGYLPA
                     RRASRVDPIKALRYE"
BASE COUNT         1755 a         3478 c         3661 g         1633 t
ORIGIN      
        1 gcgagccgca cgccgaaacg cgcctcgacc gcctcgatga tgtccccggc aaatgagagc
       61 agctcggcgg tggtccctcc cagattcacc agggcgagcg tgtgttgcgg cgagacgccg
      121 acgccccctt tccggaaccc ccgggtgaaa cccgcccgct ccacgagcca ggccgcggag
      181 agcttcactc gcccgggtcc ggcaggatag cggggcatgg ggggcaggcc gctttcccgg
      241 cacctggtct ccagtgcctc aaaggcggct gcatcgatca ccgggttggt gaagaaggac
      301 cctgccgacc gatgtgaggc cgcggactcg atgagcatgc ccttgctccg tcggattgcg
      361 agcaccgcgt cccggacttc acggagcgtg gccgagggtc gcacggcctt caccaggtcc
      421 gcgtaggcga tctgcggcgc tccctccggt gtgaggcgaa gggagacggc gagcacgacg
      481 tagcgactcc ggtcacgctc cttgaaccgg ctggtgcgat acccgaattc gcactccccc
      541 gggccgaagg tatggaccgc gagagtggta cgatcgagac aggtgacgga acgaagggtg
      601 ctcgcgatct cctggccgta ggcgccgaca ttctggatgg gagtcccacc caccgtgccc
      661 gggatgcccg agaggcactc cacgccggcc cagttccgtg cgacagcgcc ttggaccagg
      721 gtgtcccagg agaccccggc ttcagcctcg acttcgccgt tcgactcccg gaactccaag
      781 ccattggttg tgacgtggag aaccaatcct ctcaggccct catccgggag gacgacgttg
      841 ctcccgccac ccaggatggt gactccgagg cgatgcgcgg ccgcccagcg caacgcctcg
      901 acgatttcgt ctgtggtgcg gcattcagcg aggtactggg ccgggccgcc cagtccgagg
      961 gtcgtgaggg gggcgagggg gacgcgttcg ctgatgaatt cgggcacgga cacgggaaga
     1021 aggatagcga gtcttcccgt gtccgtgccc gggcgttgcg atcagagcga tttcagaaac
     1081 gcgaccagat ccgactgctc ctgcgccgtc aggttcattt ggaaccgggc gttgtagaac
     1141 gccaccacct gctcgagtga cgctgctgcc ccgttgtgga agtagggtgc gcgtgcggcc
     1201 agaccgcgga gaatcggccc cttgatccgg ttcaggtcgg cgcactttcc ggtcagcagc
     1261 gccttgcccg ggtcactggt gaaggacgtg cctgaggggc atgcgacctc ataaatgggc
     1321 agatcggggg tcgagagctg ctgcagggcc gcgaggatat gggggtccgt ttcgtacgcc
     1381 acggcgtggc tcgtcccgat gtcgaggggc aagggcagcg agtggtcgcc gacattgggc
     1441 gcgttgtggc aggtggtaca ggtgccggtg atcgccggca ggcccagcgc gtcgttcagg
     1501 ccccggacgt tggtgatggt caacgcatgg gttccgaaaa tctgttcacc gcgggcaatc
     1561 gactgccggg ccggcgtctg cttcccgctg ttcgtcagcg acagccaccg ctggaaaatg
     1621 ctcatggacg ccggatcgaa cgcggcgccg gtgggattcc cacccaaggg gtcattgatg
     1681 cccgggtaga acggctggcc ggccagtgca ccgggacctc cgttcgcccc cgacgcgttg
     1741 agctcacccg cggcattatc agcccgctgg gcgctgtaga gggacatctc gaaatcgacg
     1801 atccccgcca cttgggcgct cgtgggcgcc accgccgcct gcgcgtgccc cagtgtcgca
     1861 tccatcgcct ggtgcgagag atcggcccgg agattcgcgg cgaaggtcgc gggattgttg
     1921 aggggcatga cggtctcacg gccgtcgaac atcacggccg tcaggaagcc gagattggtg
     1981 gccgggaggg gccgccgata gaccgagacc gtcgtgcccg tggagtcggt gtgcgcggcg
     2041 cagttgtacg ggtcgtgcac cacggagagc gtgtattgcg tgaccccctg ctcggtgatc
     2101 cccacgcgga tgagaccatt gccgatcagc aggctgtgcg cgtccgggtc attcccggga
     2161 ggcgtgttgg ggcagttcgc gccatcgacc ggcgcaaaga gcggatccgt ccccgcactc
     2221 gccgcgaaga gttgccgcgc gtaatcggcc gacaggccca tggcattccc gatgacatgg
     2281 caggaggcgc aggaacggcc gttggtgccg agtgactgga aaaaggggcc ccggagatcg
     2341 agcccgccgc tggtgctgta ggtggcgact attccggacg ggtcaggcat gggggcctgg
     2401 tttccggtcg ccgcgacgga cagagcggtg ccgcgcgccg tcgcgggctg catggggtcc
     2461 cggggctgct cgcaggcgac gagcccggcg acggccagca cggcggggat cagacggacg
     2521 gacgacatgt tacctccctc gagggtgtac gcctccaagg aggtatatcg gaactagggc
     2581 ttcaaacccg acaacctcga taggagggaa tcggcccccc gggtgagggg gtggtccggt
     2641 cccagcccat tggtcagggc cctccgggcg tttgtggcgg ctatacgggc ttgggcggtg
     2701 tctcccgagg ccagacgggc ccgggcctcc aggaggcccg cggcccctat ccagccgctc
     2761 cggcgatagg tgagggaatc ccaggaggag gtggagtcga gcatgcgggc gtagaaaaac
     2821 gcgctgtcgg tccatcccag ctgaatggcc aggtctcctg cggccatgac caccggccgc
     2881 atccggatgc gtcgcttccc ttcaaaccag ccgctccggt gcagggcggt caggtaggcc
     2941 tgctgggcca ccctcgcctg gcccgccgcc tgggcgaaca tcccttccag tgtcgtggtg
     3001 tcaggcacta cgtcatcggt gttctgcacg cgcggaaatg ccttgaggac ggttctgaac
     3061 cgcgccgcgg tggcgcgtgc cttctggaga tcgccggtcg cgatctgggc tctggccaat
     3121 ccatacagcc cgcggccaag ccagtaggag tccttctgag cgacggcccg gtccacgatc
     3181 acgccgaagt atttcgccgc ggaatccccg tgcgcctcga tcagcgccgt ttcggcgtag
     3241 tggatcagtg gctgccagtc gatccggcct gcggcgtcgg cgcgcgcggc tcgcatcagc
     3301 acatcgtgat acatgcgctc tgcttcagcc agctcaccca gtcgggccag cgcataggcg
     3361 ttgtcatgct ccatgatcac ccgttccatc atcccgcccc gcccgctcga gtccattccg
     3421 gtcagcgcgc gatggaacgt cgcgagggcg gcgcggggat ttcccccttc atcctgggca
     3481 cccgccagcc cactcagcag actgaagaac gccgcgtccc gggtctgccc gatccgggtc
     3541 ttgatggcga cggcttcgga gaatgccagg cccgcggagt cgaaatgccc cgtctccatc
     3601 aacaagtttc cgcgcgtctc caggcagccc acgctgaccg tcggatcatc cgcaccctcc
     3661 cgcagggagt ccgcacccga gaggaccttt cgggcctcat cgtagcgtcc ttcggtgcgc
     3721 agttggtcca cccgatcaca ccgcacttcg gccatgaggg cgggggaccg caagctcgtc
     3781 gcaatcgagt cggccacgtt gaggaggctg tcacggaccg ggcgggcgcc gatctcgcca
     3841 taccggtccg agaggtccac caatagagat gaaaggaagc gcggatcacc gccctggcgg
     3901 agcaggagct gccgcccgcg gtccagcagc tcccccatgg tcatcggctt gtcgccgacc
     3961 tcggacaaga ggagcgtctg gaactcgacc tgcgcgtcgg cgcgctgaga ctgataccgc
     4021 gcttcgtccc gctgcacgcg ggcggcgcgc gcctgcaggg cggtgaccac ggccgcgccg
     4081 atcagggcca gccacaccag cgcgccggcg acgacggttc cccggttccg gcgtacgaac
     4141 ttcgccgtgc gatagacgat ggtcgcccgg cgggcggtga cggggacatg ctggaggtac
     4201 cgggtgagat cgtcggcgag cgcggccacg gtctggtacc ggcggcccgg atccttgtcg
     4261 agggccttgg cgacgatgtt gtcgagatcg cccgcgtact gcgcccgcag ccggtcgatg
     4321 cccgcgccgc gcgtctccgc cgcctgggga gtcaccgaac gtgagagccg ggccggttcc
     4381 acttcgacga tggcccgggc ccgcgcggcc ggtgtgggac agtcttcgtt ggtcggatgc
     4441 cggcccgcga ggagctggta gagcaggatc cccagggcgt agacgtccgt ggcggtggag
     4501 accggctcgc cgcggatctg ctcgggggcc gccgcatcga aggtcagcat ccgggttcgg
     4561 gtgaccgtgt cgtcctccgg agcaccctcg agcatcttcg cgatcccgaa gtcgagcagc
     4621 tttaccgtgc cgtccttcgc caccaggatg ttggacgcct tgaggtcccg atggacgatc
     4681 aggttcgcgt gcgcatgggc caccgcggcg agcacttgct gaaagagagc cacccgtccc
     4741 gccacgttga gacggtgctc gtcgcaccat tggtcgatgg cctgcccgtc cacgtgttcc
     4801 agcacgaggt agggctggcc tccgggactg acgccggcgt cgaggagccg cgcgatgttc
     4861 gggtgcgtga ggcgggccag cacatgtccc tcggcccgga accgcgattc gccttcgggg
     4921 cccgacaggg ccagggccag gaacttgatg gcggcggtgg ctttgaagcg cccgtcggca
     4981 cggctggcca gccacacagt ccccatcccg ccctgcccga tcgggcgctc gagccgccag
     5041 ggacccagct cgcgggtcac gggtgaggcc aagagctcgg ccatgggtgg ggcttcggcg
     5101 ggagacagga atccttcggc atcggcacgc gcctcacggg cgaggagctg ctccagatgg
     5161 tccaccagat caggccgctc gcggcggcgt tccgccagcc aggccgcgcg tgcctccggc
     5221 tcgagctcga gtgcggcgtc gagcagggcg ttgagcgctt gccaatcgag gggatcgagg
     5281 ggtgtggtca ccgcagctga tgatggagga tgacgcgcgc cttgcgccag tcgcgctgaa
     5341 tggtgcgctc agagacgccc cgcatgcccg ctatctccgt gaacgagaga ccgcagaaga
     5401 aatgcagatc gacgagctcc gccagcggcg ggtccagggt cgccagctcg tccagcgcca
     5461 gccccagcgc atcgagctcg gacggaagca caccaggccc ttcctccgag ccgtcgaggg
     5521 ggaggagctg gaactcgcct ccccgtttgg cggccgagcg gcgccgggcg tagtcgatga
     5581 tgagaccgcg catggcgcgt gaagcgtacg agaggaaacg ggagcggtcg gggaaccgga
     5641 gctggtcgtt gtccgcgaga ctgagatacg cctcgtgaag caacgtggtc gtgctgaggg
     5701 tggaaccgta gggcagtccg cggagccgcc gctgcgccag ggcgtgcagc tcgtggtaga
     5761 gtgcggcgaa cagctgatcg gatgtaccgc tcatgaccga aagccttgga cggcgatcgc
     5821 caatatgacc ctagggtgtg gcgctcacca gaacctggag tgcaaggggc gcgtcacgcc
     5881 cgaacacctc cttcgccggt tgggcctgga tctgggggtg aggaccgggt gcgccgcgcg
     5941 ggccgtcacg gcccgcggag ccacgccggc ccgaaggccc gatgggacca tcggcgcacg
     6001 acaatcgtgt caccggcggg tgcctcccac ccatgccccc ttcaccaccg ggtccaccct
     6061 ggccgccagg tcctcctcgc cctccctctg agcgggcgtc gatgagactg gcgaacagcg
     6121 gccaatcgtc ggggccgacg atggtgacct gcccaccgcg cccccccggt ccgccattcc
     6181 cgcccggacc gccaccctgc cccgggcctc ccggatcacc gggacatccg tcgcctccac
     6241 gatctccgtt ccttccggga acgcccggag ctccgatccc tccctggcca cccaccgaca
     6301 cgacggagat ccagccggac ggcggcacct tggatgcatc cgccaccacg tagaacggag
     6361 gcgcgtccag gacccgaatc cccgcgacga tcagcgcctt gtagtagcgg gtgcgcacga
     6421 ctccgatgga gaccgacacg gccggaccga ctgcgccgcg gcctcccggc tctccgggcg
     6481 cgccctcgaa tcggaatccg cgcgccaggc attcgtactc cggcgggatc acgagcgtat
     6541 cggcgacggt cgtgtcggtg cgcagcgtcg cgatgagttg aaagcccgag ttgaggctgt
     6601 tgatcggatt cgggtcggga gcccaatcgc cggtgacgag tggagtggcg tcgggactct
     6661 cgagacgcac cagctccgga gggaggcggt ggggaagcca ggacccgtcg tccagctgtc
     6721 cgtcgtatgt ggcggcgaac gcctttccgg ggcaggcgct ggccggcgcc tcgatgcgga
     6781 ggtgcctgat ccgcgcgagc tggcccggct ccggcatcac cgccggctcg gggcccgcat
     6841 agcacgcggc caggatcaac ccggcgatcg gaagattgcg taggaagccc gtcacggctc
     6901 caatactgct caaaagccgg gtcgactgtt aggcggattc ccctggtccg aaaatggcgc
     6961 ctagtgcggg cgcagctttt cgaagagcga gtcggaccac gtagccagtt gggcggttcg
     7021 atgcgtctcg cgtacggcct gatcgtacgt cgccagaaaa cgcgccggcg cgcacatctc
     7081 ggagatcagc cggggtcgcc cgtaggtttt ttcgacgacg accgccattc gccatcccac
     7141 cgtataccag ggtccctgga tgccaaagaa gctcatgccg tgctccgcga tcgagtcggt
     7201 cgggatccgg tggtcgagga tgtcgaaaaa gaaagcctgc acccgcgcga gatccgcatt
     7261 gaagtttcca gcgtcgtgat tccaccgggc ccgcgtcgcg gagtcgtcga gggcgtgggg
     7321 atccacgtcg ggaccgccgg ccgccgcgag catggcgtat ccctccccga agcccgtcat
     7381 ccactgctcc gtccgccaca cggccgaatc ccgcaccgct cccgtgtcgc ggcatgccgc
     7441 cgtgatgccg acgtggtgca gctcgtgggc catggtgttc tccgtctggg gcccgctctt
     7501 cgacgggtcg acatacagga agatcgcccg ctcaccgtcg aggtcgaaca cgaagctgtt
     7561 gccctgtggc ttgatctcga agaacacggt ggaatgaagc ggagtgccgg gcgggaggta
     7621 ggcgagcgcc agcgcagccg cgtcgtgcac atcgatgtgc cgccaggcat ccagcgtcgc
     7681 gatgatctgc ggtgcgcggg ccaacaggga atccgagagg acgaaggccc ggaaccggtc
     7741 ctccgagaag ggacgcttga acgaggtctc ccgccgcgcc agcgcccggt agccggcgga
     7801 ggaaaagagg cgcagccagt cagcatcggt gggggctcgt cctgcctgac gcagccggag
     7861 gatggcgacg acggcgtccg gctcgtcggt caccagacga acatcgaccg cggggatccg
     7921 tggtgcggcc gactgcatca ggagcgcgag cgttacggcg tgaaccatga aggcgtccgg
     7981 cctggggtca gactgtccga cacgcttcgg acgctcaggg tttcgttggc gccagagaca
     8041 ggctccagat gtgggtattc ccggtgacgt agacggtgcg atgatcgggg ctgaggtggg
     8101 cgccccaggg ggcgtccttg tttatctcgt cgccgagatc ctgcggggca ctccagtggc
     8161 ccccttcgcg gaaggcgatc gaaagccgtc cgaggttccc gtctcccttc ccgtaatcga
     8221 aaacgagaaa agactcgtcc ggtgcgatgg cggggtcgtg cgtgggctgg gcggagtcgc
     8281 ccagcgcaac gcgagtagcg gggaggtatg caccgttccg gtattcagac cggtagatgt
     8341 gcatcgcacc gcccaggcgc tggatgaaat agaccgcccc attcgcggcg acactggggg
     8401 cgacgacgag gttcgagccg ttgaccccgg accccagcca gaccggcgtc ccccagccat
     8461 ctccgtggcg gtccaccctc cagaggttgg ctccgcggta cttcttgcca tcctgctcga
     8521 acacgacgga atcgccgccc ggcaccgggc ggttggagct gaagaccagg taggagccgt
     8581 ccggcgaaac cgcgggatcc tgatcgagcc accggcccga aaacggcacc gtaaccggtg
     8641 cgctccagtg cccggcctgc caatgcgaag ccatgatcga cttgtcgtgg ccgatggtcc
     8701 ggtcgaagta gaacgttcct ccgtcaggcg tgaatgcggg cgagtcgtca tgctcggcac
     8761 tgaggatgcc gtccatgacg atcaccggcg tggcggactg attttgagct tgcatcaggg
     8821 caacgccgag tactccgagg atcatcggga tcatggaaaa acgttcaacg cttgcggccg
     8881 acaagtaaac cccggcttgg cgcggggtga cagagtcttg gcgctacggg cgagagatgc
     8941 ggcgcgaggg acaggtcact cgtatcgcag tgccttgatc ggatcgactc gcgaggcccg
     9001 gcgagccggg aggtagccag ccgccagcgc gaccagcgtg agcaccatga gcgaaagggc
     9061 gaacactcca gggtcggtgc cgttcaaccc gaagaggagc gagctcgccg ccctgccgaa
     9121 taccacggcg ctcgcgagtc cgatgacgcc gccgatcacc atcatcccgc cgacctgtct
     9181 gagcaccatc attcgcacgc gtcccgcgtc ggcgccgagc gccatacgga caccgagctc
     9241 ccgcgtccgc tgggccacgg aatacgcgag gatgccgtac aggccgaccg cggccaggag
     9301 tgtcgccagc acggcgaacg cggtcgagag aatcgagatc atgcggtcca tgtacacgtt
     9361 gtcccggacc tgctgcgcca gcgtcctgag tccctcgacc gggatgcccg gatcgatctt
     9421 ccgcacgacg cccgggatcg agcgcatcac ggcctccggc cgggcggtcc gcaggtagaa
     9481 gctcatcgag ggaatgtccg ggcgctgccg ccagggcaga tagaacaccg ccggcacggt
     9541 cgccttgacc tctgaatact tcacgtccgg cacgacgccg acgatctgga tgttggcggt
     9601 gtcgggggga tcggagaagt atttcccgac ggcgccgctc gtcaggccga acttcttcac
     9661 gaacgcctga ttcaccacac ccacgcgcgg cgcgcctcga cgatcggcgt ccgtgaattc
     9721 ccgcccggcg aggaggtgca cgccgagtgt cgagaagtag ttggtgccca cgaggttgta
     9781 gctcgcgcct cgatcgacgt cgggcccgct ggggaatcct gccgtgtaca cggggcggcc
     9841 tctgttacgg ccggcgaaga tgggcacgtt cgacgtcgtc agcgacgtca cacccggaat
     9901 gcctgccacc gcctcctcga cgcgggcata gtacgccgcg gtccggggat cctgatagcc
     9961 cgtctgctcc ggggacaatt ggaacgtgac gaccgaatcg atgtggatcc cgaggtccgc
    10021 gcgcgcgacg ttcacgaggc tcttcatgaa caggccggcg gcgatgagca gcgcggtcgc
    10081 gagcgagatc tggagcgtca ccagcgacga gcggaaacgc gcggccgccc gcgctccgga
    10141 gatatgcccc gcgttcgccc ggatggtggt taccagatcg ccgcgggtgc tgtgccacgc
    10201 ggggaataga ccaaaggcga tgcccgtggt cacggtgagc accgcggaga acagcagcac
    10261 cgaaggctgg agcgtgaagg ccaagcctcc gctggtagcg gcgggaatca acgaggcgac
    10321 cccggtgagc gtccacttcg cgaccaacag gctcgccact ccgccgaggc acgcgagggc
    10381 gagtgactcc accagcagct gggcgatcaa gcgtgcccgg ctggcgccga gggccagccg
    10441 gagacccatc tccatggcgc gtccggcgcc acgggcgagc aacagattcg cgatgttggc
    10501 gcaggcgatg agcagcacga tcccggt
//
LOCUS       DASUBP010000221         2002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1301279, whole genome shotgun sequence.
ACCESSION   DASUBP010000221 DASUBP010000000
VERSION     DASUBP010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2002
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301279"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1163
                     /locus_tag="VFU75_08480"
     CDS             <1..1163
                     /locus_tag="VFU75_08480"
                     /inference="COORDINATES: protein motif:HMM:NF015052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HEU5466913.1"
                     /translation="PLVANGALKGISFDFGSTLFEWLEQHAPETYEGVIAADRATGNA
                     IAQPYHHVILPLATRRDKVTEVRWGIADFRRRFGRTPDGMWLPETAVDEETLDVLALE
                     GIQFTVLAPHQVSGAPPRGAPGRFKTSGGRSIVLFPYDGGLAQGVAFGESLKDAGVWA
                     DQLANRDADLVTIATDGETYGHHKKGGDQVLADLIARLRSRSDVMLENYSSYLKRHPV
                     TAEVALVAPTSWSCAHGVERWRSDCGCKMAFDQPSQQKWRTPLREGLNWLAERVHGIY
                     EQRSAGKVTDPWAARDAYGEVVGRRDRPEAPLLELEKYALRMFTSCGWFFDDIGGIEA
                     IQILRYAARAIEMSGADKPALEAGLLDFLGRAQSNDPAKGSGRDVYLNLVLH"
     gene            complement(1160..>2002)
                     /locus_tag="VFU75_08485"
     CDS             complement(1160..>2002)
                     /locus_tag="VFU75_08485"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5466914.1"
                     /translation="DITMELRLAEHPATIMGDPSQIEQVLMNLVVNARDAMPDGGRLI
                     IETADVDLDEAAAAQLVATPGPSVRLTVTDNGAGMTDETRRRLFEPFFTTKEAGKGTG
                     LGLATVYGIVKQSGGAIDVESVPGQGASFRLYFPRSTETAGAKTDRSPRDSWGRGLEG
                     LNILLVEDEPALRNAFSRMLARLKCRVTIAANGADALEIVERGEIKPDLLITDTVIPG
                     IGGNELARRLTRALPQLKVLRMSGYADPEISKAESSEAPGAFVQKPFTLPELAQAVEQ
                     AMGG"
BASE COUNT          319 a          660 c          679 g          344 t
ORIGIN      
        1 gaccgctggt cgcgaatggg gcgctgaagg ggatcagctt cgacttcggc tcgacgctct
       61 tcgagtggct cgagcagcac gctccggaga cctacgaagg cgtcatcgcc gccgaccgtg
      121 cgaccggcaa cgccatcgcc cagccctatc atcacgtcat cctgcccctc gccacccgtc
      181 gggacaaagt cacggaagtg cggtggggaa tcgcggactt tcgccgccgg ttcggccgga
      241 ctccggatgg catgtggctg ccggagacgg ccgtcgatga agagacgctc gacgtgctcg
      301 ccctcgaagg gatccaattc acggtgctgg ctccgcacca ggtttcgggt gcgcctcccc
      361 gcggtgctcc ggggagattc aagacgtcgg ggggacggag catcgtgctc ttcccgtacg
      421 acggcggtct cgcccagggg gtggcgtttg gagagtcgct gaaggacgcc ggggtctggg
      481 ccgatcagct cgcgaaccgg gacgccgacc tcgtgacgat cgcgaccgac ggagagacct
      541 acgggcatca caagaagggt ggggaccagg tgctcgccga cttgatcgcc aggctgcgat
      601 cgcggtccga cgtcatgctc gagaactact cgtcctatct caagcgccat cccgtgacgg
      661 cggaggtagc gctggtggcg cccacctcat ggagctgcgc gcatggggtc gaacgctggc
      721 gctcggactg cggctgcaag atggcgttcg accagccgtc acagcagaag tggcggactc
      781 ccctgcgcga ggggctgaat tggctggccg agcgcgtgca cgggatttac gagcagcgga
      841 gcgccgggaa agtgacggac ccgtgggcgg cgcgggacgc gtacggggaa gtcgtggggc
      901 ggcgcgatcg acccgaggcg ccgcttctcg agctcgagaa atacgcgctc cggatgttca
      961 cgtcgtgcgg ctggttcttc gacgacatcg gcgggatcga ggcgatccag atactccggt
     1021 acgccgcccg agccatcgaa atgtcgggag cggacaagcc cgcgctcgag gccgggctac
     1081 tcgactttct cggccgggcg cagagcaacg atcccgcaaa aggcagcgga cgggacgtct
     1141 acttgaacct cgtgctgcac tagccgccca tcgcctgttc caccgcctgc gccagctccg
     1201 gcagggtgaa cggtttttgc acgaaggctc ccggcgcctc gctcgattca gccttgctga
     1261 tctccggatc ggcgtaccca gacattctca ggaccttgag ctgcggcagg gcccgcgtga
     1321 gacgtctcgc gagctcgttt ccaccgatgc ctggtatgac ggtatcggta atcaagagat
     1381 ctggcttgat ctcaccgcgc tcgacgatct ccagtgcgtc ggccccgttg gcggcgatgg
     1441 tcacccggca cttgagccgc gccagcattc gtgagaacgc gtttcgcagg gccggttcgt
     1501 cctcgaccag caggatgttc aacccctcga gcccgcggcc ccacgaatcc cgggggctcc
     1561 ggtccgtttt ggcgccggcg gtctcggtcg agcgggggaa gtagagccgg aacgacgcgc
     1621 cttgcccggg tacgctctcc acgtcgatgg cgccccccga ctgcttcacg atcccgtaga
     1681 ccgtcgcgag tcccaggccg gtgcccttgc cggcttcctt ggtcgtgaaa aacggttcga
     1741 agagccggcg tctggtctca tcggtcattc ccgcgccgtt gtcggtcacc gtcaggcgca
     1801 ccgatggacc gggggtcgcc acgagctgag cggcggcggc ctcatccagg tcgacgtccg
     1861 ccgtttcgat gatcagccgc ccgccgtcgg gcatcgcgtc gcgggcgttc accaccaggt
     1921 tcatcagcac ctgctcgatc tggctcggat cccccatgat cgtggccgga tgttcggcga
     1981 gccggagctc catcgtgatg tc
//
LOCUS       DASUBP010000222         2595 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_89260, whole genome shotgun sequence.
ACCESSION   DASUBP010000222 DASUBP010000000
VERSION     DASUBP010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2595)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2595)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2595
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89260"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /locus_tag="VFU75_08490"
     CDS             complement(<1..546)
                     /locus_tag="VFU75_08490"
                     /inference="COORDINATES: protein motif:HMM:NF014609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEU5466915.1"
                     /translation="MGLKIVQVDAFTDRPFAGNPAAVCIVDAPPDPQWMQSVAREMNL
                     SETAFLSGRPDGFNLRWFTPAAEVDLCGHATLATAHVLWSEAHLPADATARFHTRSGL
                     LTAERRGDWIELDFPAQGAAPHDPPPGLLAALGVRPQFVGKNKTDFLIEVASEQDVRA
                     LAPDFGKFRDLPIRGVIVTSRS"
     gene            570..1910
                     /locus_tag="VFU75_08495"
     CDS             570..1910
                     /locus_tag="VFU75_08495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5466916.1"
                     /translation="MIVQNWWYTTLLGIQNPLQPPLTDQIRTDVLVVGAGMSGLTAAW
                     RLALAGRKVVLLDRNICGGSSTGRSAGFLTPDSELELSQILRRYGPERARDLWDAPVR
                     GIELITEAVKQHGIPCDLLSQDSLFVGSDARGWDDANAEVEARKKLGYPQTLYPRDQL
                     PSVLGTTEYYGGIRYPGTYGINPLLYAQGLKRVLLELGVRIHESTEARSWEDHTVRTH
                     LGSVTADQIIFCIDKPDRSLTPLEHNVFHAQTFLAISEPLGSAALQQLFPSGARLQCW
                     DSDLVYTYYRLTGDDRLLVGGGSALTTFALHAINTPGVMTHVLNGFQKKFPFLDRLRF
                     IQYWPGLIDTTRDLLPTIVRDPDAPWVQWVLGCVGLPWAAFCGDFVARQAAGTGAADA
                     ERYYRYFEAGRPFLVPTWLERLIGKPLVFALNNAWAKYVQVDRGKRIPDRADSL"
     gene            complement(1914..>2595)
                     /locus_tag="VFU75_08500"
     CDS             complement(1914..>2595)
                     /locus_tag="VFU75_08500"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEU5466917.1"
                     /translation="PVMATAGRVYTALREGIVRGTLAPGARLVEVEIAERLGVSRTPV
                     REALTRLEREGYVATPSGKRARPMVAPLTKDDVSELLYIVGDLEGLAARRAALLPDTP
                     RRTLLKSLTRINQSFLRAAKKRADFNRLYEADEEFHRKYVEAGAGPRLRALHEAVKPQ
                     AERYIRMYVSLLAGEAPVSAGEHQAIISAIRAGDAPAAERAVQTNWRHAAERLSRAVG
                     DAGERGSW"
BASE COUNT          405 a          869 c          883 g          438 t
ORIGIN      
        1 ggagcgactg gtgacgatca cgccccggat ggggaggtcg cggaacttgc cgaagtcggg
       61 tgcgagggcg cgcacgtcct gttcgctggc gacctcgatg aggaagtcag tcttgttctt
      121 cccgacaaac tgcggacgga cgccgagcgc ggcgagcagc cccgggggcg ggtcgtgagg
      181 cgcggcgccc tgggcgggga agtcgagctc gatccagtca ccacgccgct cggccgtgag
      241 caggccggac ctggtgtgaa agcgcgcggt cgcgtccgcg ggaaggtgcg cttcggacca
      301 gagtacgtgc gccgtggcga gtgtcgcgtg gccgcacaga tcgacttcgg ctgccggcgt
      361 gaaccaacgc aggttgaaac cgtcgggacg gcccgaaagg aacgcggtct ccgatagatt
      421 catttcacgg gccacggact gcatccactg cggatccggg ggcgcgtcca cgatgcagac
      481 ggcggcgggg ttcccggcaa agggccggtc ggtgaacgca tccacctgca cgattttcaa
      541 tcccatgcgc tgactcccgg agtgacggga tgatcgttca aaattggtgg tacacgactc
      601 ttctcggcat ccagaacccg ctccagccgc cgctcacgga tcaaatccgg acggacgtcc
      661 tggtggttgg cgctggcatg tccggactca ccgccgcgtg gcggctggca cttgcaggcc
      721 ggaaagtcgt cctgctcgac cgcaacatct gcgggggctc gtcgacgggg cgcagcgccg
      781 gcttcctcac tccggactcg gagctcgagc tctcccagat cctgcggcgg tacggtcccg
      841 agcgtgcccg ggacctctgg gacgccccgg tccgcggcat cgagctcatt accgaggcgg
      901 tgaagcagca cggcatcccg tgcgatctcc tgagccagga cagcctcttc gtcggttccg
      961 acgcgcgcgg ctgggacgat gccaacgccg aagtcgaggc ccggaagaag ctgggctatc
     1021 ctcagacgct ctatccccgc gaccagctgc cgtcggtcct cggcaccacc gaatactatg
     1081 gcggtatccg gtatccgggt acgtacggca tcaatccgtt gctctacgct caggggctga
     1141 aacgcgtcct gctcgagctg ggtgttcgca tccacgagag cacggaagcc cgttcatggg
     1201 aggaccacac ggtccggacg cacttgggat ccgtcaccgc cgatcagatc attttctgca
     1261 tcgacaagcc ggaccggtct ctaacacccc tcgagcacaa cgtcttccac gcccagacgt
     1321 ttctcgccat ctcggagcca ctgggctcgg cggctctgca gcagctcttc ccgtccggcg
     1381 cgcggctgca gtgctgggat tccgacctcg tctacaccta ctaccgtctc accggcgatg
     1441 atcgtctgtt ggtcggcgga ggcagtgccc tgacgacgtt cgcgctgcac gccatcaaca
     1501 cgccgggcgt catgacccac gtgctgaacg gatttcagaa gaagtttccg ttcctcgatc
     1561 gcctccggtt catccagtac tggccgggtt tgatcgatac gacccgggat ctcctcccga
     1621 cgatcgtccg cgatcccgac gcaccgtggg tgcaatgggt gctcggatgc gtgggcctgc
     1681 cctgggcggc gttctgcggt gatttcgtcg cgcggcaggc tgcggggacg ggggcggccg
     1741 acgccgagcg gtattaccgg tacttcgaag cgggcaggcc ctttctggtg cccacgtggc
     1801 tcgagcgcct gatcggcaag ccgctggtct tcgcgctcaa caacgcctgg gccaagtacg
     1861 ttcaggtgga ccggggcaaa cggatcccgg accgcgccga cagtctgtaa cctctaccag
     1921 ctgccccgtt cgcccgcgtc gccgacggcg cgggaaagcc gctccgccgc atgccgccag
     1981 ttggtctgaa ccgcccgctc cgcggcgggg gcgtcccccg cccggatcgc cgagatgatc
     2041 gcctggtgct cgccggcgga cacgggcgct tcgcccgcca gcagactcac atacatccgg
     2101 atgtaccgct cggcctgcgg cttgaccgct tcgtggagcg cgcggagccg gggtccggcg
     2161 cccgcctcga cgtatttccg gtggaactcc tcgtccgctt cgtagagccg attgaagtcc
     2221 gcacgcttct tggcggcgcg cagaaagctc tggttgatcc gggtcaggga cttgaggagc
     2281 gtccggcgcg gagtgtcggg gaggagcgcg gcccgccggg ccgccaatcc ctccaggtcg
     2341 cccacgatgt aaaggagctc cgagacgtcg tccttggtga gcggagcgac catgggacgg
     2401 gcgcgcttgc ctgagggcgt ggccacgtag ccttcgcgct cgaggcgggt cagcgcttcc
     2461 cggacggggg tccgggagac ccccagacgc tcggcgatct ccacctcgac gaggcgggcg
     2521 ccgggggcga gggtgccgcg cacgatgcct tcgcgaagtg cggtgtaaac gcgtcctgca
     2581 gtcgccatga cgggg
//
LOCUS       DASUBP010000223         5442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_48252, whole genome shotgun sequence.
ACCESSION   DASUBP010000223 DASUBP010000000
VERSION     DASUBP010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5442
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48252"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2417)
                     /locus_tag="VFU75_08505"
     CDS             complement(<1..2417)
                     /locus_tag="VFU75_08505"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5466918.1"
                     /translation="METLIQDLKYAVRSLRNSAGFTTVAILTLALGIGANAAIFSVVN
                     GVLLEPLPYKDPGQLVTVNHYYPSLNSLRAPVSAPGFRDYSARTDLFSAAAVENYVAT
                     NLTGAGEPERILVAKVTGEFFPTLGVAPALGRALQPDEAAAGKDHVAVLSYGFWQRKF
                     GGSPDALRQSLILNNESYQIVGVMPSTFKDFFAGGTDVYTPKWFQPADLADNRRTNEF
                     LPFVARLAPGVTLEKAQADMHALAVQLRTQYTANYAPDWDLKVTSLGVANAQTLRTSL
                     LLLLGAVAVVLLIACANVANLQLARTAARARDVAVRVALGASPRRLIRLLLTESLVLA
                     LAGGGLGILLAIWGVPALLALNDGNLPSTAHVSISLPVLGFALLVSLATGFLFGLAPA
                     MQMARTDLHESLKEGGRGAVSQRGSLAMRRGLVVSTVALALTLLVGAGLLIRSFAEVV
                     GVDPGFKPDHLLTFNLSLPPAKYANDTVRLEAFNRLTGALAAAPGVVSAGGTSNIPFA
                     GNFSTGSFNIEGYQVPANAPGPWGDIRAVTPGYFATMKAPLVAGRQFTDADRQDAPAV
                     CIVDQEMVNRYWPHTDPIGKRITFSSLSGPNIQWITVVGVVGHLAHEGLDAQKRVQLY
                     LPLAENPRAFMGYVVRTVGDPLAEVATIRRAVAAVDPDLPLAAPKSMDSLIDSSLGSR
                     RFAMLLLGGFALLALVLASVGLYGVMSYTVTQRSRELGVRLALGAGTNDVLGLVLRQG
                     VNLALVGVVVGLSAAFLLTRVMNSMLFGVGATDPITFVTIPLVLMAVAALATYLPARR
                     ATRTDPIV"
     gene            complement(2440..3102)
                     /locus_tag="VFU75_08510"
     CDS             complement(2440..3102)
                     /locus_tag="VFU75_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5466919.1"
                     /translation="MIQLRNIEKSYPVAGGQSFVLRQITMDIHGGEFVTIMGPSGAGK
                     STLLAILGMLDGAWTGEYQLLDQQVHKMKPKDRVALHKKYVGFVFQQYHLLDDLTVAE
                     NLDIPLSYRDIKKAERQAVVADTLDRFGMVAKKDLYPRQLSGGQQQLVAVARAVIHKP
                     QLLLADEPTGNLHSEQGREIMELFKLLNAGGTTIVQVTHSESNAAYGNRIVNLKDGWL
                     AK"
     gene            complement(3099..>5442)
                     /locus_tag="VFU75_08515"
     CDS             complement(3099..>5442)
                     /locus_tag="VFU75_08515"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5466920.1"
                     /translation="LFFRPPALVKDPSRVRAVDFTVTSRTFGTYTAPLGTYPRLVDFQ
                     RRAHSFAGVAAYAGHQFSLGTGEGAQKVDAILVSAQFFTVAGIRPVLGRFFSDSESSA
                     AQAAHVAVISSEFWRRQFGGAASVLGTTLQLGRSLYTIVGIAPPKFGGFEMHVPDVWM
                     PMGAAAPEALWGHVLTCDGCFWFGGVVGRLAPNVTVAQAAAEATALYRNYVPAQGKPQ
                     DSDSTATARLSSLNQAMVGEGPTGPLSIWLAAVCGIVLLIACANVANLLLARAVQRRR
                     EIALRVALGSSRGRLIRQLFVESALLAVLGGAAAVLVAMWAGPVLRAAIISQANAADA
                     VDTRMLIFTAAGALVTAVLAGVAPALHAITPDISGALKSGAREGHFQRSFTRTGLLVG
                     QVALTLVLLTGAGLFVATLRHVQGLRLGFDADRLIVASVDLDGMGLKKPAILDMYRRI
                     QNRVAQVPGVTSASLSIGTPFQSSWAIGLKVPGRDSLPSVKTGGPYIDAVTPEYFRTM
                     GTAIRQGRSFTDADTWGAEHVAIVNETFAKMVWPGESALGKCLLIGGDEEKKQTPACT
                     LVVGVAEDSRRSDVREETNLQYYVPLAQSDSLFSDGPSSLLVRTVGASRDYEANVRRA
                     VQATDPSLPFPSVSPMPQLFANRLRPWRLGSSLFSLFGGLGLLLSAIGLYGVLSYMVS
                     QRTSELGIRVALGAARRDLLTMIVSQGLKVTAIGLAIGVVGSLVAGKALNSLLYGVSP
                     HDPVVLTLVTVVLVLVATLASYFPALRATRVDPMVALRHE"
BASE COUNT          944 a         1742 c         1945 g          811 t
ORIGIN      
        1 acgatcgggt ccgtccgggt cgcccgccgg gccggcaggt acgtcgcgag cgcggcgacc
       61 gccatcagca cgagcgggat ggtgacgaac gtgatcggat ccgtcgcccc gacgccgaag
      121 agcatgctgt tcatcacgcg cgtgagcagg aacgcggccg acaggccgac cactacgccg
      181 acgagggcga ggttcacgcc ctgccggagc accagtccca gtacgtcatt ggtaccggca
      241 ccgagggcca agcggacgcc gagctcgcgc gaccgctggg tgacggtgta gctcatgacg
      301 ccgtagagtc ccaccgaggc gagcaccagg gccaggagcg cgaagccacc gagcaggagc
      361 atcgcgaacc ggcgggagcc gagcgaggag tcgatcaagg aatccatgct cttcggcgcg
      421 gcgaggggca ggtccggatc cactgccgcc acggcccgcc ggatcgtcgc cacttcggcc
      481 aaggggtcgc cgaccgtgcg caccacgtat cccataaagg cgcggggatt ctccgcgagg
      541 ggcaggtaga gctgcacgcg cttctgcgcg tcgaggccct cgtgggcgag gtgcccgacc
      601 acgccgacca ccgtgatcca ctggatgttc ggtccggaga gtgacgagaa ggtgatccgc
      661 ttgccgatgg gatcggtatg aggccagtac cggttcacca tctcctggtc cacgatgcag
      721 accgccggcg cgtcctggcg gtcggcatcg gtgaactggc gtccggcgac gagcggcgcc
      781 ttcatcgtgg cgaagtaccc gggcgtcacc gcgcggatgt ctccccaggg accgggcgca
      841 ttcgcgggca cctgatagcc ttcgatgttg aagcttccgg tggagaaatt tccggcaaac
      901 gggatgttgg acgtaccgcc cgcgctgacc acacccggcg cggccgccag ggcaccggtg
      961 aggcgattga aggcttcgag ccggaccgtg tcgttggcgt acttcgcggg cggcagcgag
     1021 aggttgaagg tcagcagatg atcgggcttg aagcccgggt ccacgccgac gacctcggca
     1081 aagctgcgaa tcaggagtcc ggcgccgacc agcagcgtga gcgccaacgc caccgtcgag
     1141 acgacgagac cgcgccgcat cgcgagcgag ccccgctgac tgacggctcc gcgcccgccc
     1201 tccttcagcg actcatggag atcagtccgc gccatctgca tggccggtgc caggccgaac
     1261 aggaagccgg tcgccaggga cacgagcagc gcgaacccca gcacgggcag actgatcgag
     1321 acgtgcgccg tggacgggag gttgccgtcg ttcagggcga gcagggccgg cacaccccag
     1381 atggcgagca ggatgccgag tccgccaccg gcgagcgcca ggacgaggct ctccgtgagg
     1441 aggagccgga tcagtcgtcg tggggacgcg ccgagggcca cccggaccgc gacatcacgg
     1501 gcgcgggccg cggtgcgcgc gagttggagg ttcgcgacgt tcgcacacgc gatgagcagg
     1561 acgacggcca cggcgcccag gaggagcagg agactggtgc gcaacgtctg cgcgttggcg
     1621 acccccagag acgtcacctt caggtcccag tcgggcgcgt agttggcggt gtattgcgtc
     1681 cggagctgca ccgccagcgc gtgcatgtcc gcctgggcct tttcgagcgt cacgccgggc
     1741 gcgagccggg cgacgaaggg caggaactcg ttggtccggc ggttgtcggc cagatccgcc
     1801 ggctggaacc atttcggcgt gtagacgtcg gtcccgccgg cgaagaagtc cttgaacgtg
     1861 gacggcatga cgcccacgat ctggtagctc tcgttgttca ggatgagtga ctgcctcagc
     1921 gcatccggag agccgccgaa tttccgctgc cagaatccgt agctgagtac cgcgacgtgg
     1981 tccttgccgg ccgccgcttc atcgggctgc agcgcccggc ccagggcagg cgccacgccc
     2041 agggtcggga agaattcgcc cgtcaccttg gccacgagga tccgctcggg ctctccggca
     2101 ccggtgagat tcgtcgccac atagttctcg acggcggccg cggagaacag gtcggtgcgt
     2161 gcgctgtaat cccggaatcc gggggccgag actggcgcgc ggaggctgtt cagagacgga
     2221 taatagtgat tgacggtgac cagctgcccc ggatccttgt agggcagggg ctcgagaagg
     2281 acaccgttca ccacggagaa gatcgcggcg ttggcgccga tgccgagcgc cagcgtgaga
     2341 atcgccacgg tagtgaagcc tgcggagttc ctgagcgaac ggacggcgta cttcaggtcc
     2401 tggatgagcg tctccatggg atctccttgc gggctgccgt tacttcgcca gccagccgtc
     2461 cttcagattc acgatccggt tgccgtaggc ggcgttcgat tcggagtgcg tcacctgcac
     2521 gatcgtggtg cccccggcgt tcagcagctt gaagagctcc atgatctccc ggccctgctc
     2581 ggagtggaga ttgccggtgg gctcgtccgc gaggagcaat tgcggcttgt ggatgaccgc
     2641 gcgcgccacg gcgacgagct gctgctgtcc gcccgagagc tgccgcgggt acaggtcctt
     2701 cttcgccacc atgccgaacc ggtcgagcgt gtcggccacg acggcttgcc gctccgcttt
     2761 cttgatgtcc cggtacgaca gggggatgtc gagattctcg gcgaccgtga gatcgtcgag
     2821 caggtggtac tgctgaaaga cgaagccgac gtatttcttg tgcagcgcca cgcggtcctt
     2881 cggcttcatc ttgtgaacct gctggtcgag cagctggtat tcgccggtcc acgcgccgtc
     2941 gagcatgccg aggatggcga gcagggtcga cttgccggcg cccgagggcc ccatgatggt
     3001 gacgaactca ccgccatgga tgtccatcgt gatctgacgc aggacgaagc tctggccgcc
     3061 ggccacgggg tagctcttct cgatgtttcg cagctggatc attcgtgcct cagcgcgacc
     3121 atcgggtcga cgcgagtggc gcggagcgcg gggaaatagc tggcgagtgt cgcgacgagc
     3181 accaggacca cggtcacgag cgtgaggacc accgggtcgt gcggggagac cccgtacagc
     3241 aacgagttga gcgccttgcc ggcgaccagc gatccgacga cgccgatcgc gagcccgatc
     3301 gccgttacct tgagcccctg actcacgatc atggtgagga ggtcgcggcg cgcggcgcca
     3361 agggcgacgc ggatgcccag ttccgacgtg cgctgggaca ccatgtagct caatacgccg
     3421 tagaggccga tggccgagag gagcaaaccc aggccaccga agaggctgaa caacgaggag
     3481 ccgagccgcc atggacgcag gcggttcgcg aagagctgcg gcatcggact gaccgacggg
     3541 aaggggaggg acgggtcagt cgcctgcacg gctcgccgca cgtttgcttc gtagtcgcgt
     3601 gaggccccca cggtgcggac gagcaaggag ctgggcccat cggagaacag cgagtccgac
     3661 tgggcgaggg ggacgtagta ctggagattg gtctcctccc gcacgtcgct ccgccgtgag
     3721 tcctcggcca caccgaccac cagggtacac gcgggggtct gcttcttttc ttcgtcgccg
     3781 ccgatcaaga gacacttgcc gagcgcgctc tctccgggcc acaccatctt cgcgaaagtc
     3841 tcgttcacga tggcgacgtg ctcggcgccc caggtgtcgg catcggtgaa tgaccgcccc
     3901 tgccggatcg ccgtgcccat cgtacggaag tactccgggg tcaccgcgtc gatataaggc
     3961 ccgccagttt tcacgctggg cagggagtcg cgtcccggga ccttgagtcc gatggcccag
     4021 gacgattgaa agggcgtgcc gattgagaga gaggcgctgg tgaccccggg gacctgggcc
     4081 acccgattct ggatgcggcg gtacatgtcc aggatcgccg gcttcttgag gcccattccg
     4141 tcgagatcga ccgaggccac gatcaggcgg tccgcgtcga agccgaggcg gagaccttga
     4201 acatgacgca gggtggcgac gaagagcccg gccccggtca acaggacgag tgtgagcgcg
     4261 acttgcccga cgagcagtcc ggtgcgcgtg aacgaccgct ggaagtgccc ttcccgggcc
     4321 cctgacttca gtgctcccga gatgtccggg gtgatcgcgt gcagggcggg tgccaccccg
     4381 gcgagcacgg cggtaaccag ggcaccggcc gccgtgaaga tcagcatccg ggtgtcgacg
     4441 gcgtccgcgg cgttggcttg cgagatgatg gctgcccgca ggacggggcc ggcccacatc
     4501 gccaccagca cggctgcggc gccaccgagg acggccagga gcgcgctctc gacgaacagc
     4561 tggcgaatca ggcgcccacg ggaggatccg agcgctacgc ggagggcgat ctcccgccgg
     4621 cgttgaaccg cccgcgcgag cagaaggttc gcgacgttcg cgcaggcaat cagcagcacg
     4681 atcccgcata cggcggcgag ccagatcgag agcggtcccg tcgggccttc gccgaccatg
     4741 gcctgattga gggagctcag gcgggccgtc gcggttgaat ccgagtcctg gggtttccct
     4801 tgtgccggca cgtagttgcg gtagagcgcg gtggcctcgg cggcggcctg cgcgaccgtg
     4861 acgttgggcg cgaggcgtcc tacgacgccg ccgaaccaaa agcagccgtc gcaggtgagc
     4921 acgtggcccc agagcgcttc aggggcggcc gcccccatcg gcatccagac gtccggcacg
     4981 tgcatctcga accctccgaa cttcggaggc gcgatgccga cgatggtgta caggctgcgg
     5041 ccgagttgca gcgtggtgcc caggacgctg gcggcgccgc caaactggcg acgccagaac
     5101 tcgctcgaga ttacggcgac atgggcggcc tgggcggcgc tgctctcgga atcggaaaag
     5161 aaacgcccga gaacgggccg aattccggcg acggtgaaga actgcgcgga cacgaggatc
     5221 gcgtcgacct tctgcgctcc ctcaccggtg ccgagggaga actgatgccc ggcgtaggcg
     5281 gccacgccgg cgaaggagtg tgcgcgtcgc tggaagtcga cgagccgggg ataggtgccg
     5341 agcggcgcgg tgtaggtgcc gaacgtccgc gacgtgaccg tgaaatcgac ggcgcgcacg
     5401 cggctcgggt ccttgaccag cgccggcggc cggaagaaga ga
//
LOCUS       DASUBP010000224         4160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_947778, whole genome shotgun sequence.
ACCESSION   DASUBP010000224 DASUBP010000000
VERSION     DASUBP010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4160
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947778"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..579
                     /locus_tag="VFU75_08520"
     CDS             <1..579
                     /locus_tag="VFU75_08520"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5466921.1"
                     /translation="EETKERIFNAAMKLFRSRGFEETTIEEITEKADVAKGTFFNYFP
                     RKEEVLRYLSEQWLEEVEDKLAAEFKGDALPNGDRLIEAFVDIARFYEQDRNLARFIL
                     ATWMESIYDEEDPTCQRWQALGAFVIGEMQRIGYFRADADPARMTNVLQSLYAFTIAK
                     WLHTEPAPYELRQEIRTRFRLVIEGLKGRGNA"
     gene            579..1991
                     /locus_tag="VFU75_08525"
     CDS             579..1991
                     /locus_tag="VFU75_08525"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU5466922.1"
                     /translation="MRAPALLAVAAALAAPLAAQHSSDTLRLTLDAAVSRALAQGQEM
                     RIARAQVQDAEGQVREATADGLPKVTGTVVYTHQFSSIYQNLGSGPDTGIAGAFANIF
                     KNSPFGAANTWNAQLQATQLLFDGGKFLFALNGAHYYRRAANQQAEQVAADVALNVKT
                     AYLNAVYANALVAVAEENLTQAQDQLHQVTLFHNAGTRSDYDLLQAKVDAANQEPDVI
                     QAKSGLDVAMLALKRLVNVPADQPLILATPVSPTDGMVPAVADTSVGAADTPQLEAAE
                     ANVHVQEAGVKVALADRWPTLTVGSTLSEQAFPQQFSPFGANYLRNWNAEIRLSFPIF
                     QGFKTVGSVARARAGLEQARAQRDQVRAQTAIDAVQARADVDRTFSLLSARRQTVNEA
                     DRALHLAGVRYGNGLSTRLEVSDARLARRQAEVNAVSATRDYLVALARLEHALGHPLN
                     LIQRSLNDSTPSSTAEGMQP"
     gene            1988..3064
                     /locus_tag="VFU75_08530"
     CDS             1988..3064
                     /locus_tag="VFU75_08530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5466923.1"
                     /translation="MKRYFVIAGALVLVLVVFLAARKGGIATKTAAADSIHTLVLGAS
                     DVAHVAPADLIAGVPVSGTLHPLVDIRIASPIPEVLEAVYVREGQAVAQGEALAKFRT
                     DAAGPAALSAEAAVRLDSADYVRMQNLYKEGAVSERDVQNAEVVLRAAQATAASAEKK
                     LSESTVRAPASGVITTKAVDAGDRVKDGDQLFQLANISQLEFEATVPSEFAAQVHEGN
                     AVTLAVTGNGGAQVHGRIARVNATVDPATRQLKVYVAVPNQDRKLVGGLFASGRVILR
                     QVKGAIAVPQAAVRTDSTGGASYVLLVVNGRIVRRDVEVGALDDQAGLVQITKGVAAG
                     DAVVTSPIPGLRAGDVVTVAGGER"
     gene            3069..>4160
                     /locus_tag="VFU75_08535"
     CDS             3069..>4160
                     /locus_tag="VFU75_08535"
                     /inference="COORDINATES: protein motif:HMM:NF013069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HEU5466924.1"
                     /translation="MFLSDLSIRRPVFATMMMLALVVLGLFSYRRLSIDLFPNVEFPV
                     LVIQTRYAGASPESIEREVTKKIEEAVNPVSGVKEISSTSTEGFSTIVVQFNLGTKIM
                     DAQADVRAKIDAIRQDLPKDIDPPVISRSDPSQQPIFVLSVAGQGWQLRDLTRLADEV
                     VSRRLENIPGVGNVTLVGGLKRQINVQLLPDRLNALGVSPDMVVDALNRENGDVPAGR
                     VDAGGSENLVRVAGKIKDPKDFAGLIVTSRNGVAVRLGDVARIEDSQEEERDAAYVSG
                     QRAVALEVRKTSDANTVEVTDQIRKVMAELAPTLPHGTSLAVIQDNSTWIRNSVDDVQ
                     KTLLEGAALTVFIVFLFLNSWRSTVITGLT"
BASE COUNT          732 a         1423 c         1350 g          655 t
ORIGIN      
        1 gaagagacca aagagcggat tttcaatgcc gcgatgaagc tcttcaggtc ccgcggcttc
       61 gaagaaacca cgatcgaaga gatcaccgaa aaggccgacg tcgcgaaggg cacgttcttc
      121 aactacttcc cgagaaaaga agaggtgctc cggtacctct ccgagcagtg gctcgaggaa
      181 gtggaagaca agctcgccgc cgagttcaaa ggcgatgcgc tcccgaacgg cgaccgcctc
      241 atcgaagcgt tcgtcgacat cgcgcggttc tacgagcagg atcgcaacct cgcccgcttc
      301 attctcgcta cctggatgga atccatctac gacgaagaag atcccacctg ccagcgctgg
      361 caggccctgg gtgcctttgt catcggtgag atgcagcgga tcgggtattt ccgcgccgac
      421 gctgaccccg cgcgcatgac gaacgtgctg cagagcctct acgccttcac gatcgccaag
      481 tggctccaca ccgagcccgc gccctacgag ctgcgtcagg agattcgcac tcgcttccgt
      541 ctggtgatcg aaggcctcaa aggccggggg aacgcgtgat gcgagctccc gccctccttg
      601 ccgtggcggc cgcgctcgcc gcgccactcg ccgcccagca ttcctccgac actctccgcc
      661 tcacgctcga cgccgccgtg tcgcgggcgc tggcgcaggg gcaggagatg cggatcgccc
      721 gcgcccaggt tcaggacgcc gaaggacagg ttcgtgaggc cacggccgac ggcctgccga
      781 aggtcacggg cacggtggtc tacacgcacc agttctcgtc catctatcag aacctgggct
      841 ccggccccga caccggtatc gccggcgcgt tcgccaacat cttcaagaat tcgcccttcg
      901 gcgccgccaa cacctggaac gcgcagctgc aggcgacgca gctcctgttc gacgggggca
      961 agttcctctt cgccctgaac ggggcgcact actaccgccg ggctgctaat cagcaggccg
     1021 agcaggtcgc ggccgacgtc gcgctgaatg tgaagaccgc ctacctgaac gcggtctacg
     1081 cgaatgcgct ggtggccgtc gccgaggaga acctgacgca ggcgcaggat cagctgcatc
     1141 aggtgacgct cttccataac gccggcacgc ggtccgacta cgacctcctg caggccaagg
     1201 tcgacgccgc caatcaggag cccgatgtca tccaggccaa gagcggtctg gacgtcgcga
     1261 tgctcgcgct caagcgcctg gtcaacgtgc ccgccgacca gcccctgatc ctggccaccc
     1321 cggtgtcgcc caccgacggc atggtgcccg cggtggccga cacgagcgtc ggcgcggcgg
     1381 ataccccgca gctcgaagcg gccgaggcga acgtgcacgt ccaggaagcg ggcgtgaagg
     1441 tggcgctggc cgaccggtgg cccacgctga cggtcgggag caccctctct gagcaggcgt
     1501 ttccgcagca gttctcgcct ttcggcgcga actacctccg gaactggaac gccgagatcc
     1561 ggctgtcctt cccgattttc cagggattca agacggtcgg ctccgtcgcg cgagcccgtg
     1621 ccggcctcga gcaggcgagg gcccagcgtg accaggtccg cgcccagacg gccatcgacg
     1681 ccgtacaagc ccgcgccgat gtcgaccgca cgttctcact cctttccgcc cgccggcaga
     1741 ccgtgaacga agccgaccgc gccctgcacc tggcaggcgt ccgctacggc aatgggctct
     1801 ccaccagact cgaagtgtcc gacgcccgcc tggcccgccg gcaagcggaa gtgaacgccg
     1861 tatccgccac ccgcgactac ctggtcgcgc tggcacggct cgagcacgca ctcggccacc
     1921 cgctgaacct catccagcgt tcgctgaacg attcgacgcc ttcatccacc gccgaaggaa
     1981 tgcagccatg aagcgctatt tcgtgattgc cggagctctg gtgctcgtcc tcgtcgtctt
     2041 cctggcggcc cggaagggcg gcattgccac gaagacggcc gccgccgaca gcattcacac
     2101 cctcgtcctc ggcgcgagtg acgtcgcgca cgtcgccccg gccgacttga tcgccggcgt
     2161 gccggtgtcc ggcaccctgc atccgctggt cgacatccgg atcgcctcac cgattcccga
     2221 agtcctggaa gcggtctacg tccgcgaggg gcaggccgtc gctcagggtg aagcactcgc
     2281 gaagttccgc accgacgccg ccggtcccgc ggccctctcc gccgaagcgg ccgttcggct
     2341 cgactcggcc gattacgtcc ggatgcagaa cctctacaaa gagggcgcgg tctcggagcg
     2401 cgacgtacag aacgccgaag tggtgctgcg cgcggcgcag gcgaccgccg cgtcggccga
     2461 aaagaagctg agcgagtcga cggtgcgggc ccccgcttcg ggagtcatca cgaccaaggc
     2521 cgtggacgcg ggcgaccgtg tgaaggatgg cgaccagctg ttccagctgg cgaacatctc
     2581 gcagctcgag ttcgaagcca cggtccccag cgagttcgcg gcgcaggtgc acgagggaaa
     2641 cgccgtcacg ctggcggtga ccggcaacgg gggagcgcag gtccatggcc ggatcgcgcg
     2701 cgtgaacgcg accgtggacc ccgccacgcg acagctcaag gtgtacgtcg cggtacccaa
     2761 tcaggaccgg aagctcgtag gcggcctctt cgcctcgggg cgggtaattc tgcgccaagt
     2821 gaagggggct atcgcggtcc cgcaggcagc ggtgcgcacc gactccacgg ggggcgcgag
     2881 ctacgtactg ctggtcgtca acggccgcat cgtccggcgc gacgtggaag tgggcgccct
     2941 cgatgaccag gcgggactgg tgcagatcac caagggtgtg gccgccggtg acgccgtcgt
     3001 cacgagtccc attcccggcc tccgcgccgg agacgtcgta accgtcgcgg gtggagagcg
     3061 ataagcccat gttcctctcc gacctgtcca tccgccgtcc cgtgttcgcc acgatgatga
     3121 tgctggcgct cgtggtgctc gggctctttt cctatcgccg gctgtcgatc gacctgtttc
     3181 cgaacgtgga gttcccggtc ctggtgatcc agaccaggta cgctggagcg tcgccggaat
     3241 ccatcgagcg ggaagtcacc aagaagatcg aagaggccgt gaacccggtg tcgggcgtga
     3301 aggagatctc ctccacgtcc accgaggggt tctcgaccat cgtggtgcag ttcaacctcg
     3361 gcaccaagat catggacgcg caggcggatg tacgcgccaa gatcgacgcc attcgccagg
     3421 atttgccgaa ggacatcgac ccgcccgtga tttcccggtc cgatccgtct cagcagccga
     3481 ttttcgtcct gtcggtggcg gggcaggggt ggcagctgcg cgatctcact cgcctggcgg
     3541 acgaagtcgt aagccgccgg ctcgagaata tccccggcgt cggcaatgtc acgctggtcg
     3601 gcggactgaa gcgccagatc aacgtccagc tccttcccga ccgcctgaac gcgctgggcg
     3661 tctctcccga catggtggtg gatgcactga accgcgagaa cggcgacgtg cccgcggggc
     3721 gcgtcgatgc gggtgggtcc gagaacctgg tccgcgtggc gggcaagatc aaagatccga
     3781 aggatttcgc ggggttgatc gtcacgagcc ggaacggcgt cgcggtccgg cttggtgacg
     3841 tagcccggat cgaagactcg caggaagaag agcgggatgc ggcctacgtg agcggccagc
     3901 gcgccgtggc tctcgaggtg cggaagacct cggacgcgaa caccgtcgag gtcaccgacc
     3961 agatccgcaa ggtgatggcc gagctggccc ccacgctgcc gcacggcacc agcctcgccg
     4021 tgattcagga caactcgacc tggatccgga actcggtcga cgacgtgcag aagacgctgc
     4081 tggaaggcgc ggcgctcacg gtcttcatcg tcttcctgtt cctgaactcc tggcggtcga
     4141 cggtgatcac cggccttacg
//
LOCUS       DASUBP010000225         6971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_661385, whole genome shotgun sequence.
ACCESSION   DASUBP010000225 DASUBP010000000
VERSION     DASUBP010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6971
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_661385"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..381
                     /locus_tag="VFU75_08540"
     CDS             <1..381
                     /locus_tag="VFU75_08540"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEU5466925.1"
                     /translation="TPGAWDVVVLTHVLEHLPDPLGALTKIRELLAPKGVGVLEFPNI
                     DAADAKLRRFLRRTGMHRHRYPPAYQPGHVQEFCRESFAYACGKAGLELAIWETYAVS
                     PLKYALYRLVPIGNKARVLVRRAP"
     gene            424..3693
                     /locus_tag="VFU75_08545"
     CDS             424..3693
                     /locus_tag="VFU75_08545"
                     /inference="COORDINATES: protein motif:HMM:NF027232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466926.1"
                     /translation="MSAPLRSTLLLVALASAAAAQTPGSPDGMRWREIGPTRAGRARA
                     LAGVPSQPNVFYAGFDDGGVWRSTDYGSTWQPLFDQEPTGAIGAIAVSPSNPGVIYVG
                     SGAGIIRPDLAEGNGVYKSTDAGKTWTHVGLDDTRMIANIAIDPANPDRVFVAALGHP
                     YGPNSERGVFRSTDGGKTWDKVLYKDEYTSANDVVMDPSDPQTLYAALWQQQQSFREG
                     DEFGGSGNGIFKSTDGGTTWTHLTEGLPAAIEANLAIAPSDPKVVYAMVAGVPPRGAR
                     VRGGGTTGVVGLYKSSDAGAHWAAVDDPNVTGDSAHPADPRPLIRIGGGDLPTLAVDS
                     KNPDIVYSSSTVLWRTNDGGRTWTAVRGAPGGDDYQKTWVNPQRPDVLLVVSDQGAVV
                     SANGGLSWSNWYTQPTAAMYHVSTDDAFPYRVCGGQQDSGSGCVDSRSMDGEITFHDW
                     HPVNIQEYGIAAPDPKNPDLVFGSGRTGVSLYNRTTGQTTGLGPDRTQRGTDFGRNVR
                     TMPINWSPLNSHTLFYASNVVWKSVDQGQSWTKISPDLTRQAWAVPANAGKYADSVKA
                     APLGAITALSPSPKSLGVIWAGTDDGNVQVTTDGGVTWKNVTPPSIKPWTRIFNLDAG
                     HFDTRTAYIAANTMRVDDPRPHFFRTHDGGATWTEIDGGMDSDAVANSIREDPRKPGL
                     LYAATETQVWVSTDDGNHWESLRINMPAVSVRDLQVKDDSSCLCSDLVAATHGRGFWI
                     LDDVTPLRQMTEARAGAAYLFKPETAIRVRFGTNDPTPWPPEWPAGENPPPGGIIDYM
                     LSHSTSNPVTLEILDRAGKVVRSYSSADSVLTPDPGVDPEGYDRVCQEDSRSPDCSVP
                     LYWAAPLQRLSTAAGMHRFTWDLQYDPVGPRGPGDDNGAAVPHHTYPAVNSPWAPPGT
                     YTVQLTVDGKKYTQPITLRLDPRVKTPQLGLAKLAALSKEMYEGAVEVRSAYDRARAL
                     SAALDAAGEGDAAVLKARVDSLAPPPGGRGGGRGGFRRFGGGEGPVTLQSASGDLLGA
                     AMAMQGADVAPSAGEVAACTKARGERTDVMRRWAALSGPALTALNTKRKAAGQPEVRI
                     P"
     gene            3690..5084
                     /locus_tag="VFU75_08550"
     CDS             3690..5084
                     /locus_tag="VFU75_08550"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5466927.1"
                     /translation="MTPLLLLALAAVQRPTLAPNVHPYVSVDTTVVALAHVRVIDGTG
                     AAPKDDQTLIIRDGNIVAMGSAASIKVPDGAQVMDLTGKSVIPGLVMMHEHLYYPTGP
                     GVYGADYVSFSRLYLAGGVTTMRTAGNVGGYADLNLAAAIKAGTALGPWIDATAPYVN
                     GPSPFTQMYALKDSADAQRFVAFWADAGATSFKAYMNITRPELRAAVREAHKRGLKIT
                     GHLCSVTYREAADAGIDNLEHGFFVATDFVKDKQPDLCPGQNPGMNALATVDPKSPEF
                     TSLVQHLVAHHVAVTSTLTVFETFTPGQPFPPGTDVLDPILKAQYEQGYNRISGDTST
                     RFKKLLALDRTMELAFARAGGLLIAGTDPTGGGGVIPGYSDQREAELLVGNGFTPLEA
                     IRICTLNGATYLGRADRIGSIAVGKQADLVVIDGNPAADINDIAKVTLVFKQGVGYDP
                     MKIIESVRGKVGLF"
     gene            5172..5879
                     /locus_tag="VFU75_08555"
     CDS             5172..5879
                     /locus_tag="VFU75_08555"
                     /inference="COORDINATES: protein motif:HMM:NF013209.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HEU5466928.1"
                     /translation="MPAAHVADESSARIAAAIGDPARARMLYALSDGRARTSTELALV
                     AAVGPSTASTHLSRLKRERLVRVLIQGRHRYYSLYGAGVADVLESLSVLAGRVGTGVQ
                     TATPQHLRLARTCYDHLAGTLGVLVHDRLLAADWMSGGARGYEVTAKGSRALAGLGID
                     VDALRRERRRFAFPCLDWSERRCHLGGALGAAVLGVLLKRRWVIRERRERTVSVTPLG
                     ARELKARFGVVVSERAS"
     gene            complement(5860..6594)
                     /locus_tag="VFU75_08560"
     CDS             complement(5860..6594)
                     /locus_tag="VFU75_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466929.1"
                     /translation="MRLAWFVSFVLTPGVLTAQLAPHPSVDTIVARYLAARGGAARIR
                     DTRTIRLVGHMELGQGADGIDTIEMARPGRIRTTVHLAQGTLVQAYDGKTVWGIEPFN
                     GDRTPHVADAGTAKNVIAGGDMDGPLLDSKARGIQVSFIGLDTAQGRPAWALKVVRPD
                     STVDTYYIDTASYGLTRWQGRRVQGGEPVTYETYFRDYRRYGGMLFPCRLESHTLGRP
                     GSQLIVVDSVAVDLPVSDDRFKMPAH"
     gene            6631..>6971
                     /locus_tag="VFU75_08565"
     CDS             6631..>6971
                     /locus_tag="VFU75_08565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEU5466930.1"
                     /translation="MSDLLALHVIVASTRQERKGPLVAAWFMKQVAAHAGYRVELIDL
                     ATVNLPMLDEPRHPRFRQYEHEHTKTWSATIDRADCFVFVTPEYNFSAPPSLLNALDY
                     LSQEWAYKPVG"
BASE COUNT         1175 a         2416 c         2269 g         1111 t
ORIGIN      
        1 acgccgggcg cctgggacgt cgtggttctc acgcacgtcc tcgaacatct ccccgacccc
       61 ctcggggccc tcaccaaaat ccgcgaattg ctcgctccca aaggagtcgg ggtactcgag
      121 tttccgaaca tcgacgcggc ggacgcgaag cttcgccgtt tcctcaggag gacggggatg
      181 catcgccacc gctacccccc ggcctaccag cccggccatg ttcaggagtt ctgccgtgag
      241 tcgttcgcgt acgcgtgcgg gaaagccggg ctcgagctgg cgatctggga gacctatgcg
      301 gtgagtcccc tcaagtacgc gctgtatcgg cttgtgccga tcggaaacaa ggctcgcgta
      361 ctggtccgcc gcgctccata atccttcccg tcagccatcc aatccgatcc acgagaggtc
      421 tccttgtccg caccacttcg cagtaccctg ctcctggtcg ccctcgcatc cgccgccgcc
      481 gcgcagaccc ccggttctcc cgacggcatg cgctggcgcg aaatcggccc gacccgcgcg
      541 ggccgcgccc gcgcgctcgc cggcgttccg agccagccga acgtcttcta cgcgggattc
      601 gacgacggcg gcgtctggcg ctcgaccgac tatggctcaa cctggcagcc cctgttcgat
      661 caggagccca cgggcgccat cggtgccatc gcggtgtctc cttcgaatcc cggcgtcatc
      721 tacgtcggat ccggtgccgg gatcatccgg cccgacctcg cggagggcaa cggggtctac
      781 aagtcgaccg acgccggcaa gacctggacg cacgtcggtc tcgacgacac ccgcatgatc
      841 gccaacatcg cgatcgatcc ggctaatccg gatcgcgtct tcgtcgcggc gctcggtcac
      901 ccctatgggc ccaattctga gcgcggcgtc ttccgctcca ccgacggcgg gaagacgtgg
      961 gacaaggtgc tctacaagga cgagtacacg agcgccaacg acgtcgtgat ggacccctcc
     1021 gacccgcaga ccctctacgc cgcgctctgg cagcaacagc agagtttccg tgagggagac
     1081 gagttcggcg gctccgggaa cggcatcttc aagtcgaccg acggcggcac gacctggacg
     1141 catctgacgg agggacttcc ggccgccatc gaagcgaacc tcgccatcgc gccgagtgat
     1201 ccgaaggtgg tctatgcgat ggtggcgggc gtgcctcccc gcggagcccg ggttcgaggc
     1261 ggcggcacga ccggcgtggt cgggctctac aaatcgagcg atgccggtgc ccattgggcc
     1321 gcggtcgacg accccaacgt gaccggagac tcggcgcacc ccgccgaccc ccggcctctg
     1381 atccgtatcg gcggcggcga ccttcccacg ctcgcggtcg actcgaagaa cccggacatc
     1441 gtctacagct cgtcgacggt gctctggcgc accaacgacg gcggccgcac ctggaccgcg
     1501 gtgcgcggcg cccccggtgg agacgactac cagaaaacct gggtcaatcc gcagcggccc
     1561 gacgtgctcc tcgtggtctc cgaccagggc gcggtcgtct cggcgaacgg cggactttcg
     1621 tggagcaact ggtacacgca gccgacggcg gcgatgtacc acgtctcgac cgacgacgcg
     1681 ttcccgtacc gggtgtgcgg gggacagcag gactcgggct ccgggtgcgt cgacagccgc
     1741 tcgatggacg gggaaatcac gttccacgac tggcaccccg tgaacatcca ggagtacggc
     1801 atcgccgcgc cggatcccaa gaaccccgat cttgtattcg gcagcggtcg caccggcgtt
     1861 tccctctaca accgtacgac gggccagacg accggtctcg gccccgaccg gacccagcgg
     1921 ggcaccgact tcggccgcaa cgtgcgcacc atgccgatca actggtcgcc gctcaactcc
     1981 cacacgctct tctatgcgtc gaacgtcgtc tggaagagcg tcgatcaggg gcagagctgg
     2041 acgaagatca gccccgatct cacgcggcag gcgtgggcgg tgccggcgaa cgccgggaag
     2101 tacgccgaca gcgtgaaggc cgcgcccttg ggcgcgatca cggcgctctc gccttccccc
     2161 aagagcctgg gggtcatctg ggcgggcacc gacgacggca acgtccaggt caccacggac
     2221 ggcggagtga cctggaagaa cgtcacccct ccgtcgatca agccgtggac ccggatcttc
     2281 aacctggacg ccggccactt cgacacgcga acggcctaca tcgccgcgaa caccatgcgc
     2341 gtcgacgatc cccggcccca cttcttccgc acccatgacg ggggtgcgac gtggaccgaa
     2401 atcgacggcg gcatggattc ggacgccgta gcgaactcga tccgtgaaga cccccggaag
     2461 ccgggactgc tctacgccgc cacggagacg caagtctggg tgtccaccga cgacggcaat
     2521 cactgggagt cgcttcggat caacatgccc gcggtttcag tgcgtgatct gcaggtcaag
     2581 gacgactcga gctgcctgtg ctccgatctc gtcgcggcga cgcatggccg cgggttctgg
     2641 atcctggacg acgttacccc actgcggcag atgaccgagg cgcgcgcggg cgcggcgtat
     2701 ctcttcaagc cggagaccgc cattcgcgtg cgtttcggga ccaacgatcc cacaccgtgg
     2761 ccaccggagt ggcccgcggg cgagaatcca cctcccggcg gcatcatcga ctacatgctg
     2821 tcgcactcga cgtcgaatcc ggtgacgctt gagattctcg accgggccgg caaggtggtt
     2881 cgctcgtact cgagcgccga ctcggtgctc acccccgatc cgggggtcga tcccgagggc
     2941 tacgaccggg tctgtcagga ggactcccgc tcgcccgact gctcggtgcc gctctactgg
     3001 gcggccccgc tgcagcggct ttcgacggcg gccggcatgc accggttcac atgggacctg
     3061 caatacgacc ccgtaggtcc tcgcggcccc ggtgacgaca acggtgccgc cgttccccac
     3121 cacacctacc ctgcggtcaa cagcccctgg gcgccccccg gcacctacac ggtgcagctg
     3181 acggtggacg ggaagaagta cacccagccg atcaccctcc ggctcgatcc cagggtgaag
     3241 accccacaat tggggctcgc gaagctcgcg gccctgtcga aggagatgta tgagggtgcg
     3301 gtagaggtac ggtcggccta cgatcgagcg cgggcgctct cggcggccct cgacgccgcg
     3361 ggagaaggag atgcggcggt gctcaaggcc cgcgtcgatt cgctcgcccc tccgccgggg
     3421 gggcgtggcg gtggccgcgg aggcttccgt cgctttggtg gcggcgaggg cccggtgacc
     3481 ctgcagtccg cgagcggtga tctgctcgga gcggccatgg ccatgcaggg cgccgatgtc
     3541 gcgccctcgg cgggagaagt cgcggcgtgc acgaaagccc gcggcgagcg gaccgacgtg
     3601 atgcggcgtt gggcggcgct atcgggcccc gcgctgacgg cgctcaatac gaagcggaag
     3661 gccgcaggac aaccggaggt gagaattcca tgacgccact cctgctgctg gcactcgcgg
     3721 cggtgcaacg ccccaccctg gccccgaatg ttcacccgta cgtctccgtg gacacgacgg
     3781 tggtcgccct ggcgcatgtt cgggtcatcg acggcaccgg ggccgccccg aaggatgatc
     3841 agacgttgat cattcgggac ggcaacatcg ttgcgatggg gagcgccgcc tccatcaaag
     3901 tgccggacgg ggcgcaagtc atggacctca ccggcaagtc cgtgatcccg ggtctcgtga
     3961 tgatgcacga gcacctctac tacccgaccg ggccgggtgt ctatggcgcc gactacgtga
     4021 gcttctcgcg gctctacctg gcgggtggag tcacgacgat gcggacggcc ggaaatgtcg
     4081 gcggctacgc cgacctcaac ctggccgcgg cgatcaaggc cggtacagcg ttgggcccgt
     4141 ggatcgatgc caccgcgccg tacgtcaacg gcccttctcc attcacccag atgtacgcgt
     4201 tgaaagactc cgcggacgcc caacgctttg tcgccttctg ggccgacgcg ggcgccacgt
     4261 cgttcaaggc gtacatgaac atcacccgtc ccgagctgcg ggccgccgtg cgggaagccc
     4321 acaagcgcgg gctcaagatc acggggcatc tctgttcggt gacgtaccgc gaagccgccg
     4381 acgcagggat agacaacctg gagcatggat tcttcgtcgc gacggatttt gtgaaggaca
     4441 agcagccgga cctgtgcccc ggccagaacc cggggatgaa cgcgctcgcg accgtggatc
     4501 ccaagtcccc ggagttcacg tccctcgtgc agcacttggt cgcgcaccac gtagccgtca
     4561 cgtcgacgct caccgtgttc gaaacgttca cgcccggaca gcccttcccg cccggaaccg
     4621 acgtgctcga cccgatcctg aaggcccagt acgagcaggg ctacaaccgg atctcgggag
     4681 acaccagtac acgcttcaag aagctcctgg cgctcgaccg gacgatggag ctggcgttcg
     4741 cgcgcgccgg tggactcctg atcgccggga ccgatcccac gggcggcggt ggcgtgattc
     4801 ccggatactc ggatcagcgc gaggcggagc tgttggtggg caacggattc acacccctcg
     4861 aagccatcag gatctgcacg ctgaatgggg cgacctacct cggccgggcg gaccgcatcg
     4921 gctcgatcgc cgtcggcaag caggccgatc tcgtcgtcat cgacggcaat cccgcggccg
     4981 acatcaacga catcgccaag gtcacgctgg tgttcaagca gggtgtgggc tacgatccaa
     5041 tgaagatcat tgagtcggtc aggggaaagg tcggactctt ctagtccccg tccccggtca
     5101 cctatctgtg cgtctgtgcg tctagcaggt tggtccttgc actacctgca cacttcgttt
     5161 actgtcgaac tatgcctgca gcccacgtcg ccgacgagag ctcggcccgg atcgccgccg
     5221 ccatcggcga tccggcccgg gctcgcatgc tctacgccct gtcggacgga cgggcccgca
     5281 ccagcaccga gctcgccctg gtcgccgcgg tcggcccctc gaccgccagc actcacctga
     5341 gccgcttgaa gcgcgagcgg ctggtgcgcg tgctgattca gggccgccac cggtactaca
     5401 gcctgtatgg cgccggagtc gcggacgtgc tcgaaagtct cagcgtcctc gcgggacggg
     5461 tcgggaccgg ggtgcagacc gccacgcccc agcacctgcg cctggcccgc acctgctacg
     5521 accatctcgc cggtacgctc ggcgtcctgg tgcacgaccg gctgctcgcg gccgactgga
     5581 tgagtggcgg agcgcggggc tacgaggtca ccgcgaaggg aagccgcgca ttggccgggc
     5641 tgggcatcga cgtcgacgcg ctcaggcggg aacggaggcg cttcgcgttt ccctgcctcg
     5701 actggagtga acgccgttgc cacctgggag gagcactcgg tgcggccgtc ttgggggtgc
     5761 tgctcaagcg ccgctgggtc atccgcgagc gccgggagcg gaccgtttca gtcacgcccc
     5821 tcggcgcccg tgagctcaag gcaaggttcg gcgtggtggt cagtgagcgg gcatcttgaa
     5881 gcgatcgtcg gagaccggca ggtccacggc tacggaatcc acgacgatca gctgactccc
     5941 gggccggccg agcgtgtgag actcgagccg acaggggaac aacatcccgc catatcggcg
     6001 gtagtcgcga aagtatgtct cgtaggtcac cggctcgccg ccctgtaccc gacgcccctg
     6061 ccaacgcgtg agaccgtacg aggccgtgtc gatgtagtac gtgtcgacgg tcgagtcggg
     6121 ccgcaccacc ttcaacgccc aggcaggcct cccctgggcc gtatcaagcc cgatgaacga
     6181 gacctgaatg cctcgagcct tggagtcgag gagcgggccg tccatgtccc cgcccgcaat
     6241 cacgttcttc gccgtcccgg catcggcgac gtgcggggtg cggtcgccat tgaacggctc
     6301 gattccccac accgtcttcc cgtcgtaggc ctgcaccagt gtcccttggg ccaggtgcac
     6361 ggtggtccga attcgccccg gccgcgccat ctcgatcgtg tcgatgccgt cggcgccctg
     6421 cccgagttcc atatggccga ccagccggat ggttctggtg tcccggatcc gggcggcgcc
     6481 ccctcgcgcc gcgagatacc gcgcgacgat ggtgtcgacg gacggatgag gggcgagctg
     6541 agccgtcagc acccccgggg tcagcacgaa cgacacgaac caggccagtc tcacggttgc
     6601 actccctgca taaggtgtag tttgaacgtc atgagtgatt tgctcgcgct gcacgtgatc
     6661 gtcgccagta cccggcagga gcgaaagggt ccgttggtgg cggcgtggtt catgaagcag
     6721 gtggcggccc acgccggcta ccgggtcgag ctgatcgatc tggccacggt aaaccttccg
     6781 atgctcgatg aaccgcgcca cccgcggttt cgtcagtatg aacacgagca cacgaagacc
     6841 tggagcgcaa ccatcgaccg ggccgattgc ttcgtattcg tcacgccaga gtataacttc
     6901 agcgcgcccc cctcccttct caacgcgctg gactatctga gccaggagtg ggcgtacaag
     6961 ccggtcggat t
//
LOCUS       DASUBP010000226         2408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_143457, whole genome shotgun sequence.
ACCESSION   DASUBP010000226 DASUBP010000000
VERSION     DASUBP010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143457"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(92..2062)
                     /locus_tag="VFU75_08570"
     CDS             complement(92..2062)
                     /locus_tag="VFU75_08570"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU5466931.1"
                     /translation="MEFRHLRYFTAVAETRNLTAAAKRAHVAQPALSRAVQELEKELG
                     VKLLDRHTRGVSTTLAGEALASGAARLLEEVATALDRAAAVASGTRGRLVMGAMLSAI
                     ATGIPGEAEEILRRKNPEVTLVLKDSDPPDITEGALDGTFDVIVAVESGALPAPLTSE
                     PLWADAVDQALVPARHPLAKQPILEIADLAALPLVLPQRTMAPGWWRELLADLQRLGL
                     RSPLLVIDGGLRDTHFAVAAGRGWAAIGRSRAAGALPQGTVAVPLAGLTTELVGVVSW
                     RRHDRRPVLRTTIEAVLEAARKHPNSRVRAAPALPSVTLQRASGKLRRPDGTLPPAIE
                     VRHLRAMLAVAATQTIGRAAERLGITQPALSRQLGELEHASGLSLMERSARGVQLTPA
                     GTALAGDCPALLLELERILMETTRARRGMEGRCVIAAVPTATTGEILTGLLAATAETH
                     PHLHLVIEEMSTPLQVDAIRKGEIDLGLAHNYPGLDKTGSLKLDLIQDDRVDSALVSV
                     TGPLTGRKRLAASDLADVPFLFIERSFHPAFYDRIMSALRGIGLEPRLEGTYDGLRAL
                     WSIAAQGKGWCLGFRSQAKHPPSGTVLIPITGFDVPWGITLIRRKTEPSAAVQAVLDV
                     LKRLNGKKRRKGRQSGSSRLRRSELALSEAKG"
BASE COUNT          387 a          844 c          799 g          378 t
ORIGIN      
        1 cagcatcacg agatggcacc actcggcggt cagaaagggg cggggcatat cacaaacttc
       61 cacttctttt ccttacatac aacgcggcct gtcatccctt cgcttcgctc agggcgagct
      121 ctgagcgccg aaggcgcgaa gacccgcttt gccgcccctt ccttctcttc tttccgttca
      181 gtcgcttcaa cacgtccagc accgcctgca ccgcggcact cggctcggtc ttccgcctga
      241 tgagtgtgat tccccagggc acgtcaaagc cggtaatggg aatcagcacc gtgccggacg
      301 gcggatgttt cgcctgcgag cgaaatccca ggcaccaccc tttcccctga gccgcgatgg
      361 accagagcgc gcgcaagccg tcgtaggtgc cctcgagacg aggctcgagg ccgatgcccc
      421 gcagtgccga catgatccgg tcgtaaaacg cggggtggaa tgatcgctcg atgaaaagga
      481 aggggacgtc agcgagatcg gacgccgcca gccttttccg tccagtcagc ggtcccgtga
      541 cactcacgag cgctgaatcc acgcggtcat cctgaatcag atcgagcttc aggctccccg
      601 tcttgtcgag tcccggatag ttgtgggcga gccccagatc gatctctccc ttgcggatag
      661 cgtcaacctg cagcggcgtc gacatctcct caatgaccaa atgaagatgg ggatgcgttt
      721 cagccgtcgc ggcgaggaga ccggtgagga tctcaccggt ggtcgcggtg gggacggcgg
      781 cgatgacgca gcgcccctcc atgccgcggc gcgcccgcgt ggtctccatc aggatccgtt
      841 cgagctcgag caacagggcg gggcagtccc ccgccagtgc ggtccccgcc ggcgtcaatt
      901 gcacccctcg cgcgctcctc tccatcaggc tcagtccaga agcgtgctcg agctccccga
      961 gttgccgcga gagcgccggc tgggtaatcc cgaggcgctc ggcggcgcgg ccgatcgtct
     1021 gcgtcgcggc gacggcgagc atcgcccgga ggtgccgtac ttcgatcgcc ggcggcaacg
     1081 tgccgtcggg gcgccgcagt tttccactgg cgcgttgaag ggtcacggac gggagcgccg
     1141 gcgcggcacg cacccggctg ttgggatgct tccgggcggc ctccagcacg gcctcgatgg
     1201 tcgtgcgcag gacgggccgg cggtcatgcc tccgccagct gacgacacca acgagctccg
     1261 tggtcagtcc ggcgaggggc acggcgacgg tcccctgagg aagagccccc gcggcgcgcg
     1321 accgtccgat cgccgcccac ccgcgcccgg cggcgacggc gaagtgggta tcccgcaggc
     1381 cgccgtcgat gaccagcaat ggcgaccgga ggccgaggcg ctggagatcc gcgagcagct
     1441 cccgccacca tcccggggcc atggtgcgtt ggggaagcac gagaggcagc gccgcgaggt
     1501 cggcgatctc gagtatgggc tgtttcgcga gcggatgacg ggccggtacg agcgcctgat
     1561 cgacggcgtc ggcccagagc ggctcactgg tgagcggcgc ggggagggct ccactctcca
     1621 cggccacgat cacgtcgaac gtaccgtcga gcgccccttc ggtgatgtcg ggagggtccg
     1681 agtccttcag gaccagcgtg acctccgggt tcttgcgccg gaggatctcc tcggcttcac
     1741 cggggatgcc ggtcgcgatg gcgctcaaca tggcccccat cacgagccgc ccgcgcgttc
     1801 ctgaggcgac cgccgcggcg cgatcgagcg ccgtcgccac ttcctccagc agccgtgccg
     1861 ctcctgacgc gagggcctcc cccgcgagcg tggtggagac cccgcgggta tgccggtcga
     1921 ggagcttcac cccgagctct ttctcgagct cctgaaccgc ccgcgagagc gcgggctgtg
     1981 cgacgtgcgc ccgtttggcc gccgcggtga ggttccgcgt ctcggccacg gccgtgaagt
     2041 accgcaagtg ccggaattcc acgtcgtccc ccggtatgcg tccgcgctat gacatcccca
     2101 tcgaaccggt attggagtcc ttgaccccgg tcccgctaca ctgtcgccgt gcagaggggc
     2161 acgggcagcg cgcgccgtgg attcccctcc agaacccgaa acaaccgaca gccgcgcgct
     2221 cgtgaaggga ccatacggtg accagtacga aggcgccaga ggcgccccac caggccaaga
     2281 gacgccggat tctgatcgtg gacgacagct ccacgcacag gctctggctc gagctcatgc
     2341 ttggctcccg gtacgacgtc gacgtcgccg tcgacggcga agccggcctc cgagccgcgg
     2401 tcgccaac
//
LOCUS       DASUBP010000227        10189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_102861, whole genome shotgun sequence.
ACCESSION   DASUBP010000227 DASUBP010000000
VERSION     DASUBP010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 10189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10189
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102861"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1080
                     /locus_tag="VFU75_08575"
     CDS             <1..1080
                     /locus_tag="VFU75_08575"
                     /inference="COORDINATES: protein motif:HMM:NF026968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEU5466932.1"
                     /translation="GVSRGVTNHKIRWSPLERPIDYAWGIGSLARRLDAEVLVPISDE
                     STEAILENTDALPQDICLPTPSLLAYRTVCDKGLMLEQARAAGLAVPPTIVLKNREAR
                     EIPSDWFPVVLKPRKSVVTSEQLSVWGKRTKTTVAFVERPEDLGSALAKIPHAAFPVL
                     AQRRVQGAGEGVFLLRWNGEYLAEFAHRRIREKPPAGGVSVYRESVAVDPILGAAAKR
                     LLDKLDWRGVAMVECKHDDSTGEPVFMEVNGRFWGSLQLAIDAGVDFPSLLLASAFGR
                     RFSPVTRYERGIRGRWFWGDVDHLYLRLTKSPDALHLTTDRRTRREVVRDFLRHRRGD
                     HEDVFRWTDPMPFALESFRRLTLSS"
     gene            complement(982..2532)
                     /locus_tag="VFU75_08580"
     CDS             complement(982..2532)
                     /locus_tag="VFU75_08580"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HEU5466933.1"
                     /translation="MPPTSHLDRSLIHGIAWTSVIRWVTQLVSWVITLFVAHLLAPSD
                     YGLVAMALIYFGFAQLICDAGLTGAIVQTRGLKEPQLAAIGMVAGIVGIVLCALTLLT
                     APYVAAYFREGRVSGILMVLSLTFPVRALQILPRGLMAHELQFRTLAFIDGIESMVVM
                     TTTLVMALAGAGYWSLVGGPIVGTLVSTGCCFWARPHRIAWPRDLESLRPALRIGWHL
                     LVSNIAWYAYSNADFTVIGRVLGTAALGAYTLAWTIASVPVDRISSLVGRVTPAFFAA
                     VQTDPPSLRRYLALLTEGLAFVTLPLCLGLFLTAHDFVTAFLGDRWSGVTLPLQLLAL
                     YAALRSVQLLAPQVLISTGRSGRNMMFSVLALIVLPPVFYFGTRWGTAGVAMGWVTVY
                     PILVVVSFLRYALRAIEMRWSTYVAALIPAITASAAMTLAVWASRALMPAGWGVRPRF
                     GIEVAAGVVGYCGMAWVAHRERIRALSKLLRNRGSAREREPAEGFQSEGHGIGPAKDV
                     LVVPPAMP"
     gene            2670..3716
                     /locus_tag="VFU75_08585"
     CDS             2670..3716
                     /locus_tag="VFU75_08585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5466934.1"
                     /translation="MTTTMIETRVHWRLHSACPPDWSSLIDRCGAGFFHSPAGLAASG
                     EHGTPIFGELLDGDEIVGILAGVHRRCRVPPRPRHVFLPAPPAIAAGAGVTVAQAAEA
                     LRECAADAGWTELTVGSLEAAGELLVEEPAIPLPQRMEYDIGLSGTTEEMERRLTKHH
                     RRHLSEGASWVLRTLSGRDARDLAVQVTSSARQRAATHRGVRYDEVVLPPVEAFREDP
                     AWGLTTYAAMLGDVPLSAALVGWAGKRAYYVSGGSTPDGYARDASVWLHFQVALAFHC
                     AGFTHYCLGGAPASALDPKDSSHGLHRFKTGFGSQVRACTGARWVFSAEHETGHRLTR
                     WIREQFLASSKEMR"
     gene            3713..4912
                     /locus_tag="VFU75_08590"
     CDS             3713..4912
                     /locus_tag="VFU75_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466935.1"
                     /translation="MNVPPVGPKTRTVLGELWHERLGGFEISEDHAPHLHAALQWLCR
                     AQDATGTPGFARGYSLIRHEQFGTRGWQPAYPETTGYIIPTLFLAARHLGWSGLAVRA
                     IQAAHWEIEVQLPSGAVQGGVIGEPPTPAIFNTGQVIFGWLAAYHETRGDEFAAAARR
                     AADYLVSALDEDGVWRRGNSMFADRRATNYNARTAWALAEAGALFAEPRYSEAARRAL
                     RQVARQQHENGWFPACCLTDPAEPLLHTVAYAIRGLLEGGRVLGDSYLISAAAAAAGR
                     IAERVDARGTLPGRFTATWGPAAKWRCLTGEAQMTNIWLRLEAITNEKGWLRHVPQVI
                     QALKATQSRKAGDPGIRGGIKGSCPMTGWYGRFEVLNWATKFFADALMRDERPEEYAR
                     DRCGVLA"
     gene            complement(4974..5735)
                     /locus_tag="VFU75_08595"
     CDS             complement(4974..5735)
                     /locus_tag="VFU75_08595"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5466936.1"
                     /translation="MTETTELPVLEPQSEQDDADRALRGGRALELVVELAHDLRSPVA
                     AILALTELIEGGFDANPLSAGQKRQLGLIRSAALSLTSTASDVMALARRGGGLVDPQP
                     VKFSIDAVLRDVQAMLSPIAEQRGLDLVIRTSGRTERVGHPRAVGRLVLNLATNALRY
                     TNEGRVEVSVKAGQDDRVEFSVLDTGPGLSPEGSTGPMGPDRAFSTSGLGLMICRRVL
                     KELNSELKSESKKGVGTRFWFELDLPEVTTRRLDG"
     gene            complement(5732..7333)
                     /locus_tag="VFU75_08600"
     CDS             complement(5732..7333)
                     /locus_tag="VFU75_08600"
                     /inference="COORDINATES: protein motif:HMM:NF025885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HEU5466937.1"
                     /translation="MATIVSPEAPTAATEPRVAPGTVSALLALDPEAKSDIRILVVED
                     DPTLRETCASILRLDGYGVTLAGRGDEARDLLRREAFDILLTDLFLAQVPGTVLIRTA
                     LESNPDLIGIVMTGNPSVESSLDALREGAWDYLPKPFTATQLQVLIGRAAHTVLVGRE
                     TKKHAHLFDSPFEHHDQVVLLGQSPAFQSVIELAKRVAPTDASVFITGESGVGKELIA
                     QFIHRHSRRSSRSFVAVNCAALPEPLLESEMFGHVKGAFTGAVRDKAGLLETANGGTL
                     FLDELLDMPKPIQAKLLRVLQDGILRRVGSEGTDAVVNVRFICATNKDPDTAARAGDL
                     REDLYYRLRVVPIRVPPLRERTEDIPPLANHFLASYWTRYRRKGTPVPAWSEAGMLAL
                     CSHPWRGNVRELQNVIEHVAVLVSPGADIGPDDIPFENNSTPVHASVSPATLSALAED
                     TYHSARNRVISQFEIHYLSWLIDRADGNLAKAARMAGVERTTLYRMMERHGLHRTPNK
                     SLVASRVPDSAKMSDSGRQVTADAG"
     gene            7650..10076
                     /locus_tag="VFU75_08605"
     CDS             7650..10076
                     /locus_tag="VFU75_08605"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HEU5466938.1"
                     /translation="MNDNRSGLPGGLVPMPSNAPGAPPPAVVAIPVAINWQGGGQPTG
                     PDWRRTFAALWRYRWLIGVTTVVGALAGIVAGQLMHPVYQAQATIWIDQGDPRNPGPA
                     WAGRGDRLLGENAWSDLLTSFTVLENAVRSQRLYLSLKDPRDAPAFANFDVTQNLRPG
                     AYRLEVDGNGHSYTLATSEGAPLEHGELGGAVGAREGFDWTPVPSSLPAGHSILFTIS
                     TPREAAERLGAGLEVSQDEAGSFIKVGLKGAYPRTLAATLNAITQRFVEVADTLKRQR
                     LTEVRKITEEQLAEAQGNLNKAEGALQDFQIRTTTLPGGGTSAGRRNDASVGDQASSG
                     FFSMQVDREQLVQDKAAMARYLASGAGRPDSTPDAQMLQTIPSAMKAPGVAQVLDTLS
                     AKQAALRSMAYQFTDEYPPKKRLAGEVAQLERHTLPDLVRGVMRDDDARIAALGGQLG
                     QQASVLKQVPPRLIEAARLQRAVSIADDLYTSLEQRYQEAHLTENAAVADVRVLDLAV
                     APEHPLKNTAPGLLLLGLVGGCGLGLIGAVIFDRLDGRMRRAEEVSGDLGLSILGTVP
                     HLNRGHTRKVGGPESIETIEALRGLRVTLLSAFDRVGAMALTITSPGPGDGKSFISSN
                     LALACAASGLRTVLIDGDLRRGTLFRQLAAEKGPGLADYLRGKATYDRIMQATSFPSL
                     TFIARGTRGNDSPELLESSAMKNLIDDLKSRYDVVICDSPPLGAGVDSTVIGGVTGSL
                     LLVVRTGRSARSVAQTSLEILRRLPIRLLGAVINDVPQGAAYRYYPYALEYGVEPEPE
                     PEESDRVQLV"
BASE COUNT         1708 a         3387 c         3471 g         1623 t
ORIGIN      
        1 ggggtttccc gaggggtcac caaccacaag atccggtgga gccctctgga acgccccatc
       61 gactacgcct ggggcatcgg atcgctcgcc cggcggctcg atgcggaagt cctggtcccg
      121 atctccgacg agtccacgga agcgatcctg gagaatacgg acgcgctccc ccaggacatc
      181 tgtctgccga cgccatccct cctcgcttac cgcaccgtgt gcgacaaagg cctgatgctc
      241 gagcaggcgc gcgccgccgg cctcgcggtg cccccgacga tcgttctcaa gaatcgggag
      301 gcacgcgaga tcccatccga ctggttcccg gtcgtcttga agccacggaa gtcggtggtg
      361 acatcggagc agctctcggt gtggggaaag cggaccaaga ccaccgtggc gttcgtcgag
      421 cggcccgaag atctcggctc cgcactcgcg aagattccgc acgcggcgtt cccggtgctc
      481 gcgcaacggc gcgtgcaggg agcgggggag ggcgtgttcc tgctccgctg gaatggcgaa
      541 tacctcgcgg agttcgcgca ccggcggatc cgggagaaac cacccgcggg tggtgtcagc
      601 gtctaccggg agagtgtcgc cgtcgatccg attcttggcg ccgccgccaa acgcctgctc
      661 gacaaactcg actggcgcgg agtcgcgatg gtcgagtgca agcacgacga ttcgaccggc
      721 gaacccgtct tcatggaggt caacggtcgg ttctggggat cactgcagct ggccatcgac
      781 gcgggcgtgg acttcccgtc cttgctcctg gcgtccgcgt tcggccgccg gttctcgccg
      841 gtcacgcggt acgagcgcgg catccgcggt cgctggttct ggggggatgt ggatcacctc
      901 tacctccggc tcacgaaatc tccggacgcg ctgcacctca ccaccgaccg gcggacccgg
      961 cgtgaagtgg tgcgggactt cctacggcat cgccgggggg accacgagga cgtctttcgc
     1021 tggaccgatc ccatgccctt cgctctggaa tccttccgcc ggctcacgct ctcgagctga
     1081 gccgcggttt ctgaggagct tcgacagggc cctgatccgt tcgcgatggg ctacccaagc
     1141 catgccacag tacccgacca cgcccgcggc gacctcgatg ccgaagcgcg ggcgcacgcc
     1201 ccagcccgcg ggcatgaggg cgcgcgacgc ccagaccgcg agtgtcatcg ccgcggaagc
     1261 cgtgatcgcg gggatcaacg ccgcgacgta ggtgctccac cgcatctcga tggcccgcag
     1321 ggcgtaccgc aggaacgaca ccaccaccag aatcggatag accgtcaccc accccatcgc
     1381 cacacccgcg gtgccccacc tggtcccgaa gtaaaagacg ggaggcagga cgatcagggc
     1441 gagcacgctg aacatcatgt tccggccgct ccggcccgtc gaaatgagca cctgaggtgc
     1501 gagcagctgc accgaccgca acgcggcgta cagggccagc agctgaagcg gaagggtcac
     1561 gccgctccac cggtctccca ggaaagcggt cacgaaatcg tgggccgtca ggaacagccc
     1621 gagacagaga ggcagggtga cgaaggcgag cccctcggtg agcagagcca agtaccggcg
     1681 gaggctcggg ggatcggtct gcacggccgc aaaaaaggcc ggggtgaccc ggccaaccag
     1741 cgagctgatg cggtccaccg gcaccgaggc gatggtccag gcgagcgtat acgctccgag
     1801 cgccgcggtg ccgaggaccc ggccgatgac ggtgaagtcg gcgttggagt aggcgtacca
     1861 ggcgatgttg gacaccagca ggtgccaccc gatgcgaagc gcggggcgga gggactcgag
     1921 atcgcgtggc caggcgatcc ggtgcggcct cgcccagaag cagcacccgg tactgacgag
     1981 ggtgccgacg atgggcccgc cgaccagcga ccagtagccc gccccggcca gcgccatcac
     2041 cagcgtggtg gtcatcacca ccatcgactc gatgccgtcg atgaaggcga gcgtacggaa
     2101 ctgcagctcg tgcgccatga gcccgcgggg aaggatttgg agcgcccgga cagggaacgt
     2161 cagggagagc accatcagaa tcccgctgac tctgccctcg cgaaagtagg ccgcgacgta
     2221 cggcgccgtg aggagggtca gggcgcacag gacgattccc acaatcccgg ccaccatccc
     2281 gatcgccgcg agctggggct ccttcagccc acgggtctgg acgatcgctc ccgtcagtcc
     2341 cgcgtcgcag atcaattgcg cgaatccgaa gtagatgagc gccatggcca cgagcccata
     2401 gtcagacggg gcgaggaggt gcgcgacgaa gagggtgatg acccagctca cgagctgggt
     2461 gacccaacgg atgacgctgg tccaggcgat tccgtgaatc agcgagcggt cgagatgtga
     2521 cgtgggtggc atcagcccac cctcaaggca agaccaggac cagccagatg ctttggtgac
     2581 gcggcgcgcc cggtggacgt gcgatgcgtc gacgctccgc ctgggggaca cggggccgcg
     2641 gcccgggtct tgagattcat gaagtggcaa tgacgacaac gatgattgag acgcgcgtgc
     2701 attggcgcct ccactcggcg tgccccccag actggtccag cctcatcgac cggtgtggag
     2761 cgggcttttt ccactcgccc gccggactcg cggcctcggg cgagcacggg acccccattt
     2821 tcggagagct tctggatggc gacgaaatcg tcggcatcct cgccggagtg catcggcgct
     2881 gtcgagtccc cccgcgtccc cgccatgtct ttctgcccgc cccgcccgcg atcgccgcgg
     2941 gagcaggcgt cacggtcgcc caggccgccg aggcgttacg tgaatgtgcg gccgacgcag
     3001 ggtggaccga gctcaccgtg gggtccttgg aagccgccgg tgagctcctg gtcgaagagc
     3061 cggccattcc tctgccgcag cgaatggaat acgacatcgg cctctccggc accaccgaag
     3121 agatggagcg ccggcttacc aagcaccacc gtcgccacct ctccgagggc gcgtcctggg
     3181 tgctgcgcac gctttcgggg cgcgacgcgc gcgatcttgc cgtccaggtc acgagctcgg
     3241 cccggcaacg cgccgcgacc catcgcgggg tacggtacga cgaggtggtc ctccctcccg
     3301 tcgaggcatt ccgggaggac cccgcgtggg gcctcaccac ctacgccgcc atgctggggg
     3361 acgtccctct ctcggccgcc ctcgtcggtt gggccggcaa gcgagcgtac tacgtgtcgg
     3421 ggggctccac ccccgatggc tacgctcgcg acgcgtcggt atggctgcac ttccaggtcg
     3481 cactcgcgtt ccactgcgcc gggttcaccc actactgcct tgggggcgcc ccggcgtccg
     3541 ctctcgatcc caaggactcc agtcatggac tccatcggtt caagaccggg ttcggatctc
     3601 aggtgcgtgc gtgcacgggc gcccgttggg tgttcagtgc ggagcatgag acggggcacc
     3661 ggctcacccg gtggattcgc gagcaattcc tcgccagctc caaggaaatg agatgaacgt
     3721 gccccccgtc ggaccgaaga cccgtacggt gttgggagaa ctttggcacg agcgactcgg
     3781 aggctttgag atctccgagg accacgcgcc ccacctgcac gcggcgctcc agtggctgtg
     3841 ccgcgcccag gatgcgacgg ggacgccggg attcgcccga ggctactccc tgatccgcca
     3901 cgaacagttc ggcactcgtg gttggcagcc ggcctatccg gagaccaccg ggtacatcat
     3961 ccccacgctg ttcctggccg cgcggcacct tggctggtcg gggctcgcgg tccgggccat
     4021 acaggccgcg cactgggaga tcgaagtgca gctcccgagc ggtgcggtgc aggggggcgt
     4081 catcggcgag ccgcccaccc cggcgatctt caacaccgga caggtcatct tcggctggct
     4141 ggcggcgtat cacgaaacga ggggcgacga attcgcggcc gccgctcggc gggccgccga
     4201 ctacctggtg tcggcactgg acgaagatgg ggtctggcga cgcgggaact ccatgtttgc
     4261 cgaccgtcgc gccaccaact acaacgcgcg gacagcgtgg gccctggcgg aagccggggc
     4321 cttgttcgca gaaccccgtt actcggaggc ggccaggcgc gcactgcgcc aggtggcccg
     4381 ccagcagcac gagaacggtt ggttcccggc ctgctgtctc accgatccgg ccgagccgct
     4441 cctgcacacc gtggcttacg cgatccgcgg gctgctggaa ggggggcggg tcctcggcga
     4501 ctcctatctc atctccgcgg cggcagcggc ggcggggcgg atcgcggagc gcgtggacgc
     4561 gcgcggcact ctgccggggc gtttcaccgc gacgtggggt ccggcggcga aatggcgttg
     4621 cctcacggga gaggcgcaga tgacgaacat ctggctccgc ctcgaggcga tcaccaacga
     4681 gaagggctgg ttgaggcatg tgcctcaggt gattcaggca ctcaaggcga cccagagccg
     4741 gaaagccggc gatcccggga tccgcggtgg gatcaaaggc tcctgcccca tgaccggctg
     4801 gtatgggcgc ttcgaagtgc tcaattgggc gacgaagttc ttcgcggatg cgttgatgcg
     4861 ggatgaacga ccggaagagt acgcgaggga tcggtgtggg gttttggctt agacggctag
     4921 acggctagac ggctagggct acgaatgcga ggtccccacc tctccctaac cgtctaaccg
     4981 tccagccgtc tagtagtcac ttccggcaga tccagctcaa accagaatct tgttccgacg
     5041 cccttcttcg attcgctctt gagctccgag ttcaactcct tcagcactcg ccgacaaatc
     5101 atcaatccca atcccgaggt cgagaacgcc cggtccggac ccatcgggcc ggtgctgccc
     5161 tccggggaca ggcctggacc ggtatccaga acggagaact cgactcggtc gtcttggccc
     5221 gctttcacgg agacttccac ccggccttca ttcgtgtaac gcagggcgtt ggtcgccagg
     5281 ttcagcacca ggcgccccac ggcgcggggg tgtccgacgc gttccgtacg ccccgacgtc
     5341 ctgatgacca ggtcgagccc gcgttgctcc gcgatcggcg acaacatcgc ctgcacgtcg
     5401 cgcagcaccg cgtcgatcga gaacttcacc ggctgggggt ccaccaaccc gcccccccgc
     5461 cgcgccaggg ccatcacatc tgacgcggtg ctcgtcaggg acagcgcggc gctgcgaatc
     5521 agcccgagct ggcgcttctg ccccgcactc agggggttgg cgtcgaaccc gccttcgatg
     5581 agctcggtga gcgccagaat ggccgcaacg ggggagcgga ggtcatgagc caactcgacc
     5641 accagctcca gtgctcgccc gccccggagg gcgcgatcgg cgtcgtcttg ctcagattgg
     5701 ggctcgagga ccggcagttc ggtcgtctct gtcatcccgc gtccgcggtc acctgccgac
     5761 cgctatcgct cattttcgct gaatcgggca cccgcgaagc taccagggac ttgttcggtg
     5821 tccggtgcag gccgtgccgc tccatcatcc ggtagagcgt ggtgcgctcc acccccgcca
     5881 tgcgcgccgc cttcgcgagg ttgccgtcgg cgcggtcgat cagccagctg agatagtgga
     5941 tctcgaactg cgagatcacc cggttccgcg ctgagtgata ggtgtcctcg gccagggcgg
     6001 aaagggtggc cggcgagacg ctcgcgtgca cgggcgtgga attgttctcg aacggaatgt
     6061 cgtccggtcc gatgtcggca cccggactga ccagcaccgc cacgtgttcg atcacgttct
     6121 gcagctcgcg gacgttgccg cgccacgggt gcgaacagag ggccagcatg cccgcctcgc
     6181 tccacgccgg aacgggcgtg cctttccggc gataccgcgt ccagtagctc gcgaggaagt
     6241 gattggcgag aggcgggatg tcttcggtcc gctcgcgcag cggcgggacc cggataggca
     6301 ccacccgcag gcggtagtac agatcttcgc ggaggtctcc cgcacgggcc gcggtgtccg
     6361 gatccttgtt ggtggcgcag atgaagcgca cgttgaccac cgcgtccgtc ccctcgctcc
     6421 cgacgcggcg caggatgccg tcctgcagca cccggagcag cttggcctgg atcggcttcg
     6481 gcatgtcgag gagttcatcc aggaacaggg tgccgccatt cgcggtctcg agcagacccg
     6541 ccttgtcacg caccgccccc gtgaacgcac ccttcacatg cccgaacatt tcggactcga
     6601 gcaggggctc gggcagggcg gcgcagttca cggcgacgaa ggaccggctg gaccggcggc
     6661 tgtgccggtg gatgaactgg gcgatcaact ccttcccgac gccgctctcg ccggtgatga
     6721 acaccgacgc gtccgtgggc gccacccgct tggccagctc gatcaccgac tggaacgccg
     6781 gcgactggcc cagcaggacc acctggtcgt gatgctcgaa gggcgagtcg aagagatggg
     6841 cgtgcttctt ggtctcgcgg ccgaccagta cggtgtgcgc cgcgcggccg atgagcacct
     6901 gcagctgcgt ggcggtgaag ggcttcggca ggtagtccca ggcaccctct cgaagggcat
     6961 cgagactcga ctccaccgag gggttgccgg tcatcacgat tccgatcagg tccggattgg
     7021 attcgagcgc ggtgcgtatg agcaccgtcc ccggaacctg ggccagaaag aggtcggtga
     7081 gcaggatgtc gaaggcctct cgccgcagga ggtcgcgcgc ttcatcgccg cgaccggcga
     7141 gggtgacgcc gtagccgtcc agacggagga tggaggcgca ggtctcccgg agcgtcgggt
     7201 cgtcttcgac caccaggatg cggatgtcgg actttgcttc cgggtcgagc gcgagcagcg
     7261 cggagacggt cccgggagcg acgcgaggtt cggtcgccgc ggtgggcgct tcaggagaga
     7321 cgatggttgc catggctcag acttttcctc ctcagggtac cccctcatat ggcacacttc
     7381 gggccaagag tgcggggggc cccccaaata gcgacttatg tcacatggcc actgggttat
     7441 ctgcctgccg actcaaatca tcctcaacga cggtgcgcac cactctggag cgacgtccgt
     7501 cgtccgcatg caccgaattg cgaccacccg cagcgtcgca tgcgcgcgct gcgcctcgga
     7561 atcatggcga tatcctgcca ttcccccagg ctctcgctgg gcggtaccgc tcttgcggtc
     7621 ttggggggtg actcgactga ggaaaagtcg tgaacgacaa tcgcagtggt ctccccggag
     7681 gcctggttcc gatgccgtcc aacgcgcccg gtgcgccgcc cccagcggtg gtggccatcc
     7741 cggtcgccat caattggcag ggtgggggtc agcccacagg tcctgactgg cgacggacct
     7801 tcgccgcgtt gtggcgctat cggtggttga tcggcgtgac caccgtggtg ggtgccttgg
     7861 cgggcatcgt cgccggtcag ttgatgcacc ccgtctatca ggcgcaggcg acgatctgga
     7921 tcgaccaggg agacccgcgc aatccgggtc cggcgtgggc aggccggggg gaccggctgc
     7981 tcggggaaaa cgcctggtcc gacctgctga ccagcttcac cgtgctcgag aacgccgtcc
     8041 gctcgcagcg gctctacctg tccctcaagg acccgcgtga cgcgccggcg ttcgcgaatt
     8101 tcgacgtgac gcagaacctg cgccccgggg cgtaccgcct cgaagtggac ggaaacgggc
     8161 actcctacac gctcgcgact tcagaaggcg cccccctgga gcatggggag ctgggtggtg
     8221 cggtgggcgc acgcgagggc tttgactgga ctccggtccc ctcatccctc cccgccggtc
     8281 acagcatcct cttcacgatt tccacgccgc gggaagcggc ggagcgtctg ggggcgggac
     8341 tcgaggtgtc gcaggacgag gcggggtcgt tcatcaaggt cggactcaaa ggagcctatc
     8401 ccagaacact cgccgccact ctgaacgcga tcacgcaacg gttcgtcgag gtggcggaca
     8461 ccctcaagcg tcagcggctc actgaggttc gcaagatcac cgaggagcaa ttggcggagg
     8521 cgcaaggcaa cctgaacaag gccgaagggg cgctgcagga cttccagatc cggacgacga
     8581 cgttgcccgg tggcgggacg agcgccgggc gccgcaacga cgccagtgtg ggagaccagg
     8641 cctcgagcgg attcttcagc atgcaggtcg accgcgagca gctggtgcag gacaaggccg
     8701 ccatggcgcg gtacctggct tccggcgccg gccgccccga ttcgaccccc gacgcccaga
     8761 tgcttcagac tattccgtcc gcgatgaaag cccccggggt cgcacaggtc ctggacacgc
     8821 tgtccgcgaa gcaggcggca ctgcggtcga tggcctacca gttcacggac gaatatccgc
     8881 ccaagaagcg gctcgccggg gaagtggctc aactcgagcg ccacaccctg ccggatctgg
     8941 ttcgtggcgt catgcgcgac gacgacgcgc gcatcgcggc gctcggcggc cagctgggcc
     9001 agcaggcgtc agtgttgaag caggtgccgc cacgtctcat cgaagccgcc cggctccagc
     9061 gcgcggtcag tatcgcggat gatctttaca cgagcctcga gcagcggtat caggaagcgc
     9121 acctgacgga gaacgccgcg gtggctgacg tgcgcgtgct cgacctcgcg gtggcgccgg
     9181 agcatcccct caagaacacc gcgcccgggc tcctcctgct gggcctggtg ggtgggtgcg
     9241 gcctgggcct gatcggggcc gtgatcttcg accggctgga tggccggatg cgtcgcgcgg
     9301 aagaggtgtc gggcgacctg ggcctttcga tcctggggac cgtgccccac ctgaaccggg
     9361 gtcatacccg caaggttggt ggccccgagt cgatcgagac catcgaagcg ctgcgcggac
     9421 ttcgggtcac actgctctcc gctttcgatc gggtcggcgc gatggcgctg acgatcacca
     9481 gtccaggtcc cggcgacggc aagtcgttca tctcgtcgaa cctggcgctc gcgtgcgccg
     9541 cgtcggggct gaggacggtc ctgatcgacg gggacctgcg ccgcggaacc ctcttccggc
     9601 agctcgcggc cgagaaggga ccggggttgg cggactacct gcgtggcaag gcgacgtacg
     9661 accggatcat gcaggcgacg tccttcccca gcctgacgtt catcgcgcga ggcacgcgcg
     9721 ggaacgattc acccgagctg ctcgagtctt cggcgatgaa gaacctgatc gacgacctca
     9781 agtcacgcta tgacgtggtg atctgcgact cgccgccact cggcgcgggg gtggattcga
     9841 cggtgatcgg gggcgtcacg gggagcttgc tgctcgtggt gcgtacggga cggagcgcgc
     9901 ggtcggtggc gcagaccagt ctcgaaatcc tccgtcgtct gcccatccgc ctgcttggcg
     9961 cggtgatcaa cgacgtcccg cagggcgcgg cgtaccggta ctacccgtat gcgctggagt
    10021 atggagtgga accggagccg gaaccagaag agtccgatcg cgtgcaactg gtatgaccta
    10081 gacggttaga cggttagacg gctagagagg ccactgacgg ttagacggtt agacggttag
    10141 acggctagag aggccactaa ccgtctagcc gtttagccgt ctagtttgg
//
LOCUS       DASUBP010000228         4155 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1356359, whole genome shotgun sequence.
ACCESSION   DASUBP010000228 DASUBP010000000
VERSION     DASUBP010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4155)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4155)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4155
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1356359"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..749)
                     /locus_tag="VFU75_08610"
     CDS             complement(<1..749)
                     /locus_tag="VFU75_08610"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5466939.1"
                     /translation="MTPMPTAGRFCWHELMTTDRAGAIKFYGKLAGWTAEAHPGDPTY
                     QVLITRSKVRIGGVMDLPQQARQMGSPSHWVPYIEVADVDDTVRKATAMGGRTYVSPN
                     DIPNMGRFAILADNQGATFAVYKGSNGLSPDNEPGLGEFSWHEMIAGNLDSAWNFYST
                     LFGWEKREAMDMGGDLGIYQIYRNAGGTRDLGGMYTKPKDMPGGPHWLCYINVPDAAK
                     SGDYTTQNGGKVLMGPMEVPGGGHIIGGMDP"
     gene            935..1447
                     /locus_tag="VFU75_08615"
     CDS             935..1447
                     /locus_tag="VFU75_08615"
                     /inference="COORDINATES: protein motif:HMM:NF019095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HEU5466940.1"
                     /translation="MHSIHLKLALAGGLLITLATLGAAQTYGDSAKPQSKPQAFSAML
                     SGAHEVPAVNAIGTGIAKFVVKNDSTITYDITLKGVPDVTAAHIHLGAEGASGAPVAT
                     LTKGGDPGGKISGTISPKDLHGATMPQLITAMKSGGAYVNVHTKTHPDGEVRGQIQAG
                     ASATMSQATH"
     gene            complement(1504..1836)
                     /locus_tag="VFU75_08620"
     CDS             complement(1504..1836)
                     /locus_tag="VFU75_08620"
                     /inference="COORDINATES: protein motif:HMM:NF024845.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-TFIIB domain-containing protein"
                     /protein_id="HEU5466941.1"
                     /translation="MPHRNSLHRNEDEYFARREADLIRQQQEAARNERLAAERRSHFM
                     KCPACGYDLVRGEWEEHLVLDQCPHCHGVWIDAAQAHRLIEHHGNAVGWILQSVMRGV
                     AGLKTGTS"
     gene            complement(1884..2561)
                     /locus_tag="VFU75_08625"
     CDS             complement(1884..2561)
                     /locus_tag="VFU75_08625"
                     /inference="COORDINATES: protein motif:HMM:NF013786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HEU5466942.1"
                     /translation="MDHPEVCVWHAELDLPPDTISGLRDLLADDELHRVSHMRFERDR
                     RRFIVAHGALRSLLGQYLGIRPRQVQFRLKASGKPTLNRDLGSQLTFSLSHAAGLALI
                     AIANGADVGVDVEHVSYETDCLDVARHWFSAAEAHALESMPDRQRTQAFFDLWTRREA
                     WAKARGDGLAMPKTVLSAGPGREWSVFPLRPAPGYLGALAIRGSGWQLRERHWEAGVP
                     SGNTPVF"
     gene            complement(2684..3232)
                     /locus_tag="VFU75_08630"
     CDS             complement(2684..3232)
                     /locus_tag="VFU75_08630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466943.1"
                     /translation="MNPLLVLPFLAYTIAAVLWPAQISARRRGATLLGLEAVALLLLC
                     VAGWILSRLATPSVDGWRWGTVMLLGTGYLYVCGRGMALVRAVLDLPDLLMPREDDRK
                     VGGVEVVRGRAIGVLERAIALTLVLLNQYGALGLVVAAKSLARFKALEDRDFAEYFLI
                     GTLASLLLALLGGLAMRAVMRY"
     gene            complement(3229..3834)
                     /locus_tag="VFU75_08635"
     CDS             complement(3229..3834)
                     /locus_tag="VFU75_08635"
                     /inference="COORDINATES: protein motif:HMM:NF027589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SatD family protein"
                     /protein_id="HEU5466944.1"
                     /translation="MTTRYVALIADAAGSRALPAPERARLQRALTAALPDWNRRWRSH
                     LAARIALTLGDEVQALLTSPAAVWDLSHTLRYTLPQVDWVIACGLGAISTPLARTAPE
                     IDGPCFHEARAALDLAKKRRLLLAFGGFPPALNALAAYYSALHWSWTPRQRRAAIEWR
                     TGVTPRRGTDPSALSHLRRRMGWPLVTEGDRMLRVLLGESS"
     gene            3984..4056
                     /locus_tag="VFU75_08640"
     tRNA            3984..4056
                     /locus_tag="VFU75_08640"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4017..4019,aa:Phe,seq:gaa)
BASE COUNT          707 a         1302 c         1455 g          691 t
ORIGIN      
        1 tgcggatcca tcccgccgat gatgtggccg ccaccgggga cctccatggg acccatcaac
       61 accttgccgc cattctgggt cgtgtagtcc cccgactttg ccgcgtccgg gacgttgatg
      121 tagcagagcc agtgcggccc cccgggcatg tccttcggct tggtgtacat gccgccaagg
      181 tcccgggtgc cgccggcgtt gcggtagatc tggtagatcc ccagatcgcc gcccatgtcc
      241 atcgcttccc gcttctccca gccgaagagc gtcgaataga agttccaggc ggagtcgagg
      301 ttccccgcga tcatctcgtg ccaggagaac tcaccgaggc ccggctcatt gtcgggggag
      361 agtccgttgc ttcccttgta gaccgcgaac gtcgcgccct ggttgtccgc gagaatcgcg
      421 aaccggccca tgttcgggat gtcgttcggc gaaacgtagg tgcggccgcc catcgcggtg
      481 gccttccgaa cggtgtcgtc gacatcggcg acttcgatgt agggcaccca gtgcgatggg
      541 cttcccatct gccgcgcctg ctgcggcagg tccatcacgc ctccgatcct caccttgctg
      601 cgcgtgatca acacctggta cgtcgggtct cccggatggg cttcagcagt ccagccggcc
      661 agcttgccat agaacttgat ggcgccggcg cggtcggtgg tcatcagctc gtgccagcag
      721 aatcggccgg cggtgggcat gggggtcatg gtcctctcgt tcagtggaga gcggggcgca
      781 aaagttattt cggtccgcat catcccggaa gtcatcggct ggtgaggggg cgctgcgcga
      841 gggcaacaag ggtgattcgg aacttgacgc gcaccccacg gtatggtgac tgacggcaga
      901 atctgctgcc gtcaagttcc cagggaggtg cagcatgcat tcgattcacc tgaagctcgc
      961 gctcgccggc ggtctcctga tcacccttgc gactcttggc gccgcacaga cctacggcga
     1021 ttccgccaaa ccgcaatcga aaccccaagc cttcagcgcg atgctctcgg gcgcgcacga
     1081 agttccggcc gtcaatgcca tcggcaccgg catagcgaag ttcgtggtga agaacgactc
     1141 gaccatcacg tacgacatca cgctcaaggg tgtgcccgac gtgaccgcag cccacatcca
     1201 tctcggagcg gagggcgcgt cgggagcgcc agtggcgacc ctgacgaagg gcggcgatcc
     1261 gggcgggaag atcagtggaa cgatttcgcc caaggatctg catggcgcca cgatgcccca
     1321 gctgattacg gccatgaagt ctggtggtgc atacgtgaac gtgcacacca agacccatcc
     1381 ggacggagag gtgcggggac aaattcaggc tggtgcatcc gcaacgatga gccaggcaac
     1441 gcactaacgg agggccgcgt ggggaggggg gagtcgacgt cgagactctc ccccttctgt
     1501 tgcctaggaa gtccccgtct tgagacccgc gaccccccgc atcaccgact gcaggatcca
     1561 gccgaccgcg ttcccatgat gctcgatgag gcggtgcgct tgggcggcgt cgatccagac
     1621 cccatggcag tgcgggcact gatcgagaac gaggtgctcc tcccattctc cgcggaccag
     1681 atcgtagccg caggcggggc atttcatgaa atgtgagcgg cgctcggcgg cgaggcgctc
     1741 gttgcgggcg gcttcctgtt gctggcggat gagatcggct tcccggcggg cgaaatactc
     1801 gtcttcgttg cggtggagac tgtttcggtg cggcatggat cccttctcga aggcttcccc
     1861 tcatatatac ggaaaaatcc gtttcaaaag accggtgtgt ttcccgatgg gactccggct
     1921 tcccagtgcc gctcccgcaa ttgccatccg ctccccctga tcgccagcgc tcccaggtag
     1981 cctggggcgg ggcggagcgg aaagacggac cactcccggc cggggcctgc cgagaggacg
     2041 gtcttgggca tggccaagcc gtccccgcgg gccttggccc aggcctccct cctggtccat
     2101 agatcgaaga aggcttgggt gcgctgccgg tccggcatcg actccaaagc gtgggcctcg
     2161 gccgccgaga accagtgccg ggcgacatcg aggcaatccg tctcgtagct cacgtgctcg
     2221 acgtcgacgc cgacatccgc gccgttcgca atggcgatga gggcaaggcc cgccgcatgc
     2281 gaaaggctga acgtgagttg gctgccgagg tcacggttca gggtgggttt gccggaggcc
     2341 ttgaggcgga actgaacttg gcgggggcga atcccaaggt actgcccgag caggcttcgc
     2401 agggccccat gcgcgacgat gaagcgccgc cggtcgcgct cgaaccgcat gtgggagacg
     2461 cggtggagct cgtcatcggc taggaggtcg cggagcccgg agatggtatc cggtgggagg
     2521 tccaactccg cgtgccagac gcacacttcg gggtggtcca tgcccccgag agggtcaaca
     2581 ttcgtgcccg caggggaccg gccgggttgg ttgggtgccg agcgaagccg gcgggtgtgc
     2641 gcgcagcacc tgacgccccg aggcggcgcg cggttgggct ggttcagtac cgcatgacgg
     2701 cccgcatggc gagcccgccg agcagggcga ggagcaggga ggcgagtgtg ccgatcagga
     2761 agtattcagc gaagtcccgg tcctcgagcg ctttgaagcg ggcgagcgat ttggcggcga
     2821 ccacgagccc gagggcgccg tactggttca ggagcacgag tgtgagggcg atggctcgtt
     2881 ccaggacgcc gatggcgcgg ccccggacca cctcgacgcc gccgaccttc cggtcgtcct
     2941 cgcgcggcat caggaggtcg gggagatcga gcacggcgcg cacgagcgcc atcccgcgtc
     3001 cgcagacata gaggtagccg gtgcccagca gcatgacggt gccccagcgc catccgtcga
     3061 cgctgggcgt ggcgaggcgt gacaggatcc atccggcgac acacagcagg aggagggcaa
     3121 cggcttcgag gccgaggagc gtggcgcctc gccggcgtgc cgagatctgg gcgggccaga
     3181 ggacggcggc gatggtgtag gcgagaaagg gaaggacgag cagaggattc acgagctttc
     3241 tcccagcagt acccgcagca tcctatcgcc ttcggtgacc aggggccagc ccatgcggcg
     3301 ccggaggtgc gagagcgcgc tcgggtcggt gccgcggcgc ggcgtgactc cggtccgcca
     3361 ttcgatggcg gcgcggcgct ggcgcggggt ccagctccag tggagggcgg agtagtaagc
     3421 ggcgagcgcg ttcagggcgg gggggaagcc gccgaaggcc aggagcaggc gccgcttctt
     3481 cgcgaggtcc agcgcggcgc gagcttcgtg aaaacagggg ccgtcgattt cgggcgcggt
     3541 gcgagcgagg ggcgtcgaga tggcccccag gccgcaggcg atgacccagt ccacttgcgg
     3601 gagcgtgtac cggagcgtgt gagagaggtc ccacacggcg gccggactgg tcaagagcgc
     3661 ctggacctcg tcaccgagag tgagggcgat cctggcggcg aggtgggagc gccagcggcg
     3721 gttccagtcc gggagcgcgg cggtgagggc gcgctggagg cgggcccgct ccggggcggg
     3781 cagggcgcgg gagccggcgg catcggcgat gagggcgacg taacgagttg tcattttccg
     3841 tcacatatga ttgtattgac gtattacgtc aaatactatc aatatgacgt tatttgtcaa
     3901 ctaccatgtt ccgccgctgc tccccttccg gcaccactcc caccgaggca ttcgactggg
     3961 taagttcttt cgcctcatcg cccggccagg tagctcagtt ggtagagcag cggactgaaa
     4021 atccgcgtgt cggcggttcg attccgcccc tggccattcg gtaagtccca ccgacatccc
     4081 ggaaaatgga agcgccccgc caagtcccgg ggcgcttcgt gcgtcaggct cccgtcgaat
     4141 ggccccggcg ccgtc
//
LOCUS       DASUBP010000229         5650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_61915, whole genome shotgun sequence.
ACCESSION   DASUBP010000229 DASUBP010000000
VERSION     DASUBP010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5650
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61915"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(52..678)
                     /locus_tag="VFU75_08645"
     CDS             complement(52..678)
                     /locus_tag="VFU75_08645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466945.1"
                     /translation="MRPLAFAATAVLAVLAVLAVLVAIPASLSAQWSVGLSVGVARHF
                     GGAVSTADSEPGSVHPGRPTMVTLSLGREWGGGTVRADLGLGYGSPGLAVEIPGGAFI
                     DTKALHFYSGSPEITVRVLPIGSGGALRAGVGGDVLIWSITGSGSRVRLGSHLTAAYE
                     WPVAGRFLGSIQAGLSLTPSIFDASELETPFERRAMVRPSVSIGLRYH"
     gene            complement(678..1901)
                     /locus_tag="VFU75_08650"
     CDS             complement(678..1901)
                     /locus_tag="VFU75_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466946.1"
                     /translation="MRIRYGIPFVMIALGVAGIAIGCGETQPTGPTFGGVPPDSSHPG
                     ATPARNEWLARRVAIALGNPGFRAYVWAQIEHSPIREHKLQFQHFLAALNWRALHSIA
                     QAAGDSDDAVLAQAAQAQPLEFYMPVKAHLAKWHADENVLVATAVADHDAPVAFDTKG
                     RRFVLDAEHPPETPVLALVPVETDFNAPPARLQCTQETCGAGGGGDPLPPPTGLFMTA
                     SHIPNIGQFEGWLKGDPEFEVHILGQMGATDQLTDYQCAGELAGGPYTYDQNSTDWWG
                     SVLIFSQDQLNRYKLEHPKQSVRVFVVEDDDQACVLKMDLDRTHQILVAVDSASRFLS
                     GGLDSTLKLTKIFKYERIGEDLLTALASLINSNDDPVGNAVQDAVVGQFYPGYNWFLK
                     GDHNVTNGWINLEMH"
     gene            complement(1991..3154)
                     /locus_tag="VFU75_08655"
     CDS             complement(1991..3154)
                     /locus_tag="VFU75_08655"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5466947.1"
                     /translation="MFSPFVRRPRISPLTEEFLSEVRRTLRGRYEVERPLGRGGMGLV
                     YLARDIRLARPVAIKVLPPERAMSPVHRARFLREARIAARLSHPHIVPIFSVGELGDV
                     VFFVMAYVPGYTLGHRIRTRGPLPAAETARVLRETALALAHAHDQGVVHRDVKPDNIL
                     IDGSTGSSFVTDFGIARFGIGPISGSRTVLGTPEFMSPEQARGLALDARSDLYSLGVV
                     GYYAASGRLPFDAEDPYVVMTQHVTEVPSPLATVAPKTSTAVASLVDRCLEKNPDQRP
                     QSGNHIADGLAAQPAPAPHSVTRIRKLLTLGRRWSVLAPLYLVAIGVVGLPLVLAQHL
                     NLQGGLLIVVLAATWVALLPPLFLTVAQLRRLFRRIITLSEPDTAPGASPESA"
     gene            3210..>5650
                     /locus_tag="VFU75_08660"
     CDS             3210..>5650
                     /locus_tag="VFU75_08660"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU5466948.1"
                     /translation="MLPFLIAVAAAVPQDSQTPRYWQQRVAYTIDASLDETTGVLTAH
                     ERVLYVNQSPDTLHDFYVHDYLNAFRPGSRWAAYDSASGVVRYQHMKDPDYAFERITK
                     ATVMGETGAPDYPDAPDSTIAHWKLPHPLPPGGQMTVEIDWQARLSTLPRRQGRDPKT
                     RRYDFAQWYPKVVVYDEYGWEAHPLYPPGEFYGEFGTFDVTLDLASDQVVGATGVPVC
                     GDPGWEHANASAPDSVDYQRNYYAGAPHDCPPAATGRKKVRFVARDVHHFAFTLNPNY
                     VYEQGKFKDVVVHVLYQPKDRATWGNGIAVKRTEVALQWLDSTYGKYQWPQLTNVHRI
                     EGGGTEFPMMIMDGGPDQGLILHEGGHMYTMGQLANNEWREGYLDEGFTSFQTGWYFE
                     THGGRPSYDGLDGLFTSLDLDGWSEPVSMVSERYRDFNTYNLMIYNRGQFFYEELRYI
                     VGDATMHKILRTYFDRWKLKHVNEDRFRAVCEEVSGQDLGWFFGQWLHSVPIFDYKLA
                     SVERTQLPDGKWRTTVTIQRLGDGWLPVEIGDDDTIYARATGQPETERVEFVSARKPG
                     RLKLDPRLRTHDWNYLNNYEPRFLKGREATKLVFDDPTKTVTRRDSLVQGYRPQVWYN
                     DLGGLVVSLHSNQNYDGLFDRGSGDLSRETGSDASHPWGFEFSLVNPTGLRSPRQTTT
                     VSGWDVEGRAGALLRWDRTLIKSPFQPFSNPGSDPHAGFDVQWMSTTDVGYLDRRIWD
                     DAGTFELGPWYSNVARHGRTLWTTNFGVKGGIVYRVPGPGVISANRYDVNTYARLTGE
                     GSVRTPLAGDVFLGAR"
BASE COUNT         1010 a         1844 c         1887 g          909 t
ORIGIN      
        1 aagcggcccc agggtgatcc cccggggccg ctttgcttcc gctccgcttc gctagtggta
       61 cctcaatcca atgctcacgc tcggccgcac catcgctcgc cgttcgaagg gcgtttccag
      121 ttcactcgcg tcaaaaatcg agggggtgag ggacagaccc gcctgaatgc tcccgagaaa
      181 ccgcccggcg acgggccact cgtacgccgc cgtcaggtgg ctgcccagcc tgacccggct
      241 gccgctcccc gtgatgctcc agatcagtac atcccctccg accccggccc tcagggcgcc
      301 accagagccg atgggcagca cccgcacggt gatctccggc gacccggagt agaagtgcag
      361 ggctttggtg tcgatgaacg cgccacccgg gatctccacc gcgagacccg gagagccgta
      421 tcccagtccc aggtcggccc gaacggtacc cccaccccac tcgcgtccga gactcagcgt
      481 gaccattgtc ggccgcccgg ggtgtacgga cccgggctcc gagtccgcgg tggagacggc
      541 gccaccgaag tgcctcgcca cacccaccga cagtccgaca ctccactggg cgctgaggga
      601 ggcggggatg gcgacgagga cggccaggac ggccaggacg gccaggacgg ccgtcgcggc
      661 gaaggcgagt gggcgcatca gtgcatctcc agattgatcc agccgttggt gacgttgtgg
      721 tcccctttga ggaaccagtt gtagcccggg tagaactgtc cgaccactgc gtcctggaca
      781 gcgttgccca ccggatcgtc attcgagttg atcagcgagg cgagggcggt cagcaggtct
      841 tcgccgatgc gctcgtactt gaagatcttg gtgagcttca gtgtcgagtc caatccgcca
      901 ctcaagaacc gcgacgcgct gtcgaccgcc accagaatct ggtgggtccg atccaggtcc
      961 atcttcagca cgcaggcctg gtcgtcgtct tccaccacga acacccgaac gctctgcttc
     1021 gggtgctcga gcttgtagcg gttcagctgg tcctggctga agatcagcac actcccccac
     1081 caatcggtcg agttctggtc gtacgtgtac gggcccccgg caagctcgcc cgcgcactgg
     1141 taatcggtca gctgatccgt cgcccccatc tgcccgagga tgtgcacctc aaattcggga
     1201 tcccctttca accagccttc aaactggccg atgttcggga tatgcgacgc ggtcatgaac
     1261 agccccgtcg ggggcggaag gggatctccg cccccgccag ctccgcaggt ctcctgcgtg
     1321 cattgcagcc gagcgggggg cgcgttgaag tccgtctcca ccggcaccag cgccagcacc
     1381 ggcgtctccg gtggatgctc cgcgtccagc acgaagcggc gtcccttggt gtcgaacgcc
     1441 accggcgcgt catgatccgc gaccgccgtg gcaacgagca cgttctcgtc cgcgtgccac
     1501 ttcgcgaggt gggctttcac cggcatgtag aactcgaggg gctgggcctg ggccgcctgg
     1561 gccagcacgg cgtcgtcaga gtccccggcg gcttgtgcga tggagtgcag ggcccgccag
     1621 ttgagggcgg cgaggaaatg ctggaactgc agcttgtgct cgcggatggg cgagtgctcg
     1681 atctgggccc agacgtacgc ccggaacccg ggattgccca aggcgatcgc gactcgtcgc
     1741 gccagccatt cgttccgggc cggagtggcg cccgggtggg agctgtcggg gggaacgccg
     1801 ccgaacgtcg gaccagtcgg ctgggtctcg ccgcagccga tcgcgattcc cgccacaccc
     1861 agggcgatca tcacgaaggg gataccatac cgaatgcgca tacacgcctc ctgaagcgtg
     1921 aagaagggaa gcgagtatga aggagggaag agaaaaaacc gggaccaaac cctcgcgtcc
     1981 cggtacggat ttacgcggac tctgggctgg cgcccggcgc cgtgtccggt tcggagagcg
     2041 tgatgatgcg acggaagagg cgacggagct gcgcgaccgt caggaacaac ggtgggagga
     2101 gtgccaccca ggtcgccgca agcacgacga tcaagagacc tccctgcagg ttcaggtgct
     2161 gcgccagcac gagcggtaac cccacgacgc cgatcgcaac cagatagagg ggcgcgagca
     2221 ccgaccaccg tctcccgagt gtgagaagct tccgaatcct ggtgacggag tgcggggcgg
     2281 gtgccggttg agccgcgagt ccgtccgcga tgtgatttcc actctgaggt ctctggtcgg
     2341 gattcttctc caggcatctg tcgaccaacg acgccactgc cgtggaagtc ttcggagcga
     2401 cggtggcgag aggggacggc acctcggtga cgtgctgtgt catcacgacg tatggatcct
     2461 cggcatcgaa gggcaatctg ccggacgcgg cgtagtagcc cacgacgccg agggagtaga
     2521 gatccgagcg ggcgtcgagg gccagcccgc gggcctgctc cgggctcatg aactccgggg
     2581 tgcccaggac ggtgcgggac cccgagatag gaccgatccc gaagcgcgcg atgccgaaat
     2641 cggtcacgaa cgacgagccg gtcgagccgt cgatcaggat gttgtcgggc ttcacatccc
     2701 ggtggacgac gccctgatcg tgggcatgcg ccagggcgag cgcggtctcg cgcaagacgc
     2761 gggcggtctc ggccgccggc agaggaccgc gcgtgcggat acgatggccg agggtgtagc
     2821 ccggcacata cgccatcacg aaaaagacga cgtcgccgag ctctccaacg gagaaaatcg
     2881 gaacgatgtg cgggtgcgac aggcgtgccg cgatccgtgc ttcccgcagg aagcgggcac
     2941 ggtgcacggg gctcatggcc cgctcgggcg gcaggacctt gatggcgacg ggccgggcga
     3001 gacggatgtc ccgggccaga tacacgagtc ccatgccacc gcgccccagc ggccgttcga
     3061 cttcgtagcg acctcgcaac gttcggcgga cttcggagag gaattcttcg gtcagcggac
     3121 taatcctcgg gcggcggaca aagggcgaga acatgaacga gtgcccctat acccgagcgg
     3181 agcgggccgg gttccgctaa ttttttcgta tgctcccatt cctgatcgca gtcgccgcgg
     3241 ccgtcccgca ggattcacag acccctcgat actggcagca gcgcgtcgcc tacacgatcg
     3301 atgcctcgct cgatgaaacg acgggcgtgc tcaccgcgca cgagcgggtc ctctacgtca
     3361 atcaatcgcc cgacacgttg cacgattttt acgtgcacga ctatctcaac gcgttccggc
     3421 cgggatcgcg ctgggccgcg tacgattccg cgtcgggcgt ggtgcgctac cagcacatga
     3481 aggaccccga ctacgcgttc gagcggatca ccaaggccac ggtgatgggg gagaccggcg
     3541 cgccggacta tccggatgcg cccgactcga cgatcgcgca ctggaagctg ccgcacccgc
     3601 tcccgccggg cgggcagatg acggtcgaga tcgactggca ggcacgcctc tcgacgttgc
     3661 cgcgccgcca gggacgcgat cccaagaccc gccgctacga cttcgcgcaa tggtatccga
     3721 aggtcgtcgt gtacgacgag tacggatggg aagcgcaccc cctctacccc ccgggcgagt
     3781 tctacggcga gttcggtacc ttcgacgtga cgctggacct ggcgtcggac caggtcgtgg
     3841 gcgcgacggg tgtgccggtt tgcggggatc cggggtggga gcatgcgaac gcgagcgccc
     3901 ccgactcggt cgactaccag cgcaattact acgcgggtgc gccccacgat tgccctccgg
     3961 ccgcgacggg ccggaaaaag gtccggttcg tggcccggga cgttcaccat ttcgcgttca
     4021 cgctcaaccc gaactacgtg tacgagcagg gcaagttcaa ggacgtcgtg gtgcacgtgc
     4081 tctaccagcc gaaggaccgt gccacctggg ggaacgggat cgcggtgaag cggaccgagg
     4141 ttgcgctcca gtggctcgat tcgacgtatg ggaagtacca gtggccccag ctcaccaacg
     4201 ttcaccggat cgaggggggc ggcaccgagt ttccgatgat gatcatggat ggcggtcccg
     4261 atcagggtct gattctccat gagggcgggc acatgtacac catgggccag ctcgccaaca
     4321 acgagtggcg cgagggctat ctcgacgagg gcttcacgag tttccagacc ggctggtact
     4381 tcgagactca tggcgggagg ccgagctacg acgggctcga cgggctgttc acgtccctcg
     4441 atctggacgg gtggagcgag ccggtctcga tggtgagcga gcggtatcgc gacttcaata
     4501 cctacaacct gatgatctac aaccggggcc agttcttcta cgaggagctc cgctacatcg
     4561 tgggtgacgc cacgatgcac aagattctgc gcacctattt cgaccgctgg aagctgaagc
     4621 acgtgaatga agaccgcttc cgcgccgtct gtgaagaggt gtcgggacag gacctggggt
     4681 ggttcttcgg ccagtggctg cacagcgtcc cgatcttcga ctacaagctc gcttcagtcg
     4741 agcggacgca gctgccggac ggcaagtggc gcacgacggt gaccatccag cggctgggcg
     4801 acggatggct gccggtggag atcggggacg acgacacgat ttacgcccgc gcgacgggcc
     4861 agccggagac tgagcgcgtc gagttcgtct cggcccggaa gcccgggcgg ctcaagctcg
     4921 atccgcggct ccgcacgcac gactggaact acctcaacaa ctacgagcca cgattcctca
     4981 aggggcgcga ggcgaccaag ctcgtcttcg acgatccgac gaagaccgtc acgcgccgcg
     5041 actcgctggt gcaggggtac cgtccccagg tctggtacaa cgatctcggt ggcctggtgg
     5101 tctcgctgca ctcgaaccag aactacgacg gcctcttcga ccggggctcc ggcgacctga
     5161 gccgcgagac cggcagcgac gcgagccacc cgtggggctt cgagttctct ctggtgaatc
     5221 cgacgggact ccggagtccg cgtcagacca cgacggtctc cggctgggac gtcgagggcc
     5281 gggccggtgc cctgctccgg tgggaccgca ccctcatcaa gagccccttc caacccttct
     5341 cgaaccccgg gtccgatccg cacgccgggt tcgacgtcca gtggatgtcg acgacggacg
     5401 tcggatacct cgaccgccgg atctgggatg acgcagggac gttcgagctc gggccctggt
     5461 actcgaacgt cgcgcgtcat ggacggacgc tctggacgac caacttcggc gtgaagggcg
     5521 gcatcgtgta ccgcgtgccc ggccccggtg tgatctccgc gaaccgttat gacgtgaaca
     5581 cgtatgcgcg tctcaccggt gaaggcagcg tgcgcacccc tctcgcgggt gacgtcttcc
     5641 tcggcgcccg
//
LOCUS       DASUBP010000230         8373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_348287, whole genome shotgun sequence.
ACCESSION   DASUBP010000230 DASUBP010000000
VERSION     DASUBP010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 8373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8373
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_348287"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2511)
                     /locus_tag="VFU75_08665"
     CDS             complement(<1..2511)
                     /locus_tag="VFU75_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466949.1"
                     /translation="MRAGRNARLYAAVAFGAIVLGACETAANTGGVSRDNIAPRITLT
                     TPNKIHPKVDSQTISGGLSFNVTASDNIAVDSVDLTFSIGYIATHDSGFGGGPKSVTY
                     VFSLTSAQLAGAGGQILIVGTARDGAGNRSKPDTITIFLSNKQALAAFLLAPTPGAVA
                     GSGKYIPIQVKAVQQAGGIRRLGWTIGGTAGQTATTADSISTGSPPFPDSTVFSDSVL
                     VTGTSGTFQVVGFAVDSSGRRAVSAAVTVTIQSIATDNVPPEISQAVALRAEATDSIT
                     IHATDPSGITRVGFKVTDLLGAPLGGDSVNFAGTTTDQKVTLSLKLNSISVFPTQVIV
                     QAFAIDGATALNRGVTGAVFTVPSPTGASRADTVTIVAGRTFALPAGGQIGDAIYNKN
                     TNEVYLTNTALSRLEIFQVANSTFVAAGIPTSGPQPVGIALWPSDTLGNYQDTVVVAN
                     SGGTELSILDTRPAVRALAYRQALPNFVIEKYQTVNVGGTYEALITYYDVSDRPQYVG
                     TVCRAAGGTACAPDSVFAIYSTTPTASSSDPFSGRATLRMEKLTQIPANQFGHLFWEI
                     GAQLNGSVNDTLRIEEVRGLTSTVVLSACKGVTIDFANFGLGDSTFVRNSGNFTHALV
                     GEGGNISTANARVMAYNATKALNQGASTNLSCATSPLAPAGPQDAGQNDVDLGMSPAF
                     QVSDFISNTGIRISSIATNFNGLTNAVRADSIYFLDQNLRLAVTAPAPIGASGMDMNY
                     NHTFSPFGACPPCGGAGNQNDRVIFAAQPDGSIAVFDTFHGGQISSIPLRDPIIGPLR
                     VAKGPTGSQLLFGITARGLVMVAIPVINNPLPVQQAAKT"
     gene            complement(2525..4453)
                     /locus_tag="VFU75_08670"
     CDS             complement(2525..4453)
                     /locus_tag="VFU75_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HEU5466950.1"
                     /translation="MKRIAALAAGLLAAAAPTLPASGFGDGEVRAVSVLPAAGKVSVV
                     IDLRGAVQVTDFTLTNPSRLVLDLQGARLTGPSVLYDGQTRGGVKNVRYAQFKPDVVR
                     VVIELDAAKGYKIDQATGSVTVSFGADQTAFAAWSSQYNATPAPAAQPPVESSAPASS
                     QAAPQPAAEVTTPVSSNPAAVDRYLALHAREAAQSQAPRITVQWDKADIADVAAGFAA
                     FSGRTIVVGKDVKGTISAQIKNQPWDLAFQAILESQALAWQPLPGGIINVVNKADLAR
                     ADSTVPTETRLVRINYAKSTALQGAVASIVSKRGAVVADTTTNALVITDIQSRIEQDV
                     AFVQALDVRTPQVSIQAKLIFVDRTDVEDMGVKYDLGTGSGCLITGAHPCQFFNSLVA
                     RADPATLKPIDTNGDGIPDAVVATGTLPASQTVVDLGGNALSALGNAGQEVINPALSL
                     IFSTAVGNFNLTSFVQALQTVELADLQAEPSVTTSDNHTAEVLVGDLVPLRTIDAGSI
                     GGGTGSTVPRATVSFRQTGIDLKVTPHVTANRQILMEVHAENSSVKTAPVDIGFTFQT
                     QQADNQILVNDGETAVIGGLTVTNVTVSKSGIPFLVDLPILGKLFGFTSQQEERRDLL
                     ILITPHIIDDLATTSAGN"
     gene            complement(4450..4917)
                     /locus_tag="VFU75_08675"
     CDS             complement(4450..4917)
                     /locus_tag="VFU75_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466951.1"
                     /translation="MTMTRMFGVLLLAALPLSAQTPAPGAAKKDTTAAKTAAPSAAAK
                     DSATTTLLREVFSYEGSGRDPFASLLRNGDVRPLFADLKLLAIYFDGQYPGRSVAVLR
                     DITTQKRYQVKPGEVLGRLKVAQIRPRDVTFTVQEFGFERQETLSLAKPEESQ"
     gene            complement(4914..5555)
                     /gene="pilO"
                     /locus_tag="VFU75_08680"
     CDS             complement(4914..5555)
                     /gene="pilO"
                     /locus_tag="VFU75_08680"
                     /inference="COORDINATES: protein motif:HMM:NF016253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HEU5466952.1"
                     /translation="MPKDQRSQMMLILVILALGGAYAFWTYVHTPTSDAINVQQKTLD
                     SLNDVVNKAKADLASGSVEDLRRKADEYEADLGLMRQLVPEKNDVPNLLDDISTKMKI
                     RGVTQQQFAPASVDHGSPFDTYQYKLSVYGHYDQLGEFFADIASMPRIVVPQNVSLKP
                     ATPNVMKLLGDTLGGLLEADLTLRTYVKAQSAPAPAGAKKPAAAAPAKSGGAQ"
     gene            complement(5571..6290)
                     /locus_tag="VFU75_08685"
     CDS             complement(5571..6290)
                     /locus_tag="VFU75_08685"
                     /inference="COORDINATES: protein motif:HMM:NF016992.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HEU5466953.1"
                     /translation="MIEVNLLPGQKKKQAGGGAKFKLPDFKTLLASVKDPWLIAAVVS
                     WIVIGGGALGLFLLDSAKKALLDSKVTTAKHDADRYSGYIAEKHKLELHRDSLVVQIN
                     VIRQIDGQRYVWPHLMDQITKALPPYTWLQDMQQTASAPVLPPSNPSSGGAKPSSVDS
                     AAALNAVNVTLNGRTVDIQAYTTFLRQLAASPWITDVTPAAAQTVIESDRPVTQFSVS
                     FKYKQADSVFIRTVPLVQSVR"
     gene            complement(6287..7339)
                     /gene="pilM"
                     /locus_tag="VFU75_08690"
     CDS             complement(6287..7339)
                     /gene="pilM"
                     /locus_tag="VFU75_08690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HEU5466954.1"
                     /translation="MALFGFLGGKKTSVGLDVGSGIIKLVVVDHSGSEPELVKVATTE
                     VATDAIVEGEVMDPGIVAEAIRGLFSTAGVKQKAVVTAVGGRDVIVKKIQMDRMKEAD
                     AREVIRWEAEQHVPFDMANVELDFQILDPNAEGLQMNVLLVAAKRELVESRVSLLTEA
                     GLQPSVIDVDAFAIHNAFELNHPDAMNGVVGLVNIGHEVTNVNILEDGVPVLTRDLSV
                     GTRRFREDLQREKGLSAEDSEKAVTGAVQSADLASYVEQRGEEISVGVERAAAFLASA
                     SRSAGGLARVYTSGGGARIPGINEALATRLKVPVELASPIQRLSYRQEAFETVSIDEI
                     APLLMLSVGLALRRAA"
     gene            complement(7459..8226)
                     /locus_tag="VFU75_08695"
     CDS             complement(7459..8226)
                     /locus_tag="VFU75_08695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466955.1"
                     /translation="MALLSSLLMLVVSLALMAAGSVFLVNETRLSERSVSQLQEVGGA
                     EVAIGHALESWDPRNATRISEGLYLIDRSGLGLLVRLKPVAFPQGALIAGGVVSLGTG
                     TRVDGLIDSGATGTGLTGNLDLARVASMADLTIPGGRYGTLPSATGDGVIHIVGDAEL
                     GGGSGSGFLLVDGNLTVTGPLTFNGVVLVRGELVVSGAVPLSTHLSGSVVTLMGATGD
                     SSLWVIYDKALVSNVLSRFGTPERLPSRSWTSLSQVG"
BASE COUNT         1398 a         2682 c         2797 g         1496 t
ORIGIN      
        1 cgtcttcgcg gcctgctgca ccggcagcgg gttgttgatc acggggatgg ccaccatcac
       61 cagaccccgc gcggtgatgc cgaagagcag ctggctgccg gtcgggccct tggccacgcg
      121 cagcggcccg atgatcgggt cgcggagcgg gatggagctg atctgtccgc catggaacgt
      181 gtcgaacacc gcgatcgagc cgtcgggctg cgcggcgaag atcacgcgat cgttctggtt
      241 acccgcgccc ccgcagggcg ggcaggcccc gaacggtgaa aacgtgtggt tgtagttcat
      301 gtccatcccg gacgcgccga tcggggcggg tgcagtgacc gccagccgca ggttctggtc
      361 caggaagtag atggagtcgg cgcgcaccgc gttggtgagc ccgttgaagt tcgtagcgat
      421 cgacgaaatc ctgatgccgg tgttggagat gaagtccgac acctggaacg ccgggctcat
      481 gcccaggtcc acgtcgttct gtccagcgtc ctgcggcccg gccggagcga gcggcgacgt
      541 cgcgcaggaa aggttggtgc tcgctccctg attcagcgcc ttagtggcgt tgtaggccat
      601 tacccgggcg ttcgcggtcg agatgttgcc gccttcgccg acgagagcgt gggtgaagtt
      661 ccccgagtta cggacgaacg tggagtcccc gaggccgaaa ttcgcgaagt cgatcgtaac
      721 gcccttgcac gccgacagga ccacggtgct ggtaaggcct cgcacctctt cgatccgcag
      781 cgtgtcgttg actgatccgt tgagctgggc cccgatttcc cagaacaggt gcccgaattg
      841 atttgcgggg atctgggtga gcttctccat gcgcagcgtc gctcgtccgc tgaacggatc
      901 cgaactcgat gctgtcggcg tggtggagta tatggcgaaa acggaatccg gcgcgcacgc
      961 ggtcccgccg gccgcgcggc agaccgtgcc cacgtattgg ggtcggtccg acacgtcgta
     1021 gtaggtgatc agcgcttcgt acgtgccacc gacgttgacg gtctggtact tctcgatcac
     1081 gaagttcggc agcgcctgcc ggtacgcgag ggcgcgcacg gccggccggg tgtcgagaat
     1141 cgacaactcc gtaccgcccg agttcgcgac gaccacggtg tcctggtaat tacccagggt
     1201 gtcgctcggc cagagcgcga taccgactgg ttgtgggccc gaggtcggga tgcccgcggc
     1261 cacgaaggta ctgttcgcga cctggaagat ttcgagacgc gacaacgcgg tgttggtgag
     1321 atacacctca ttggtgttct tgttgtagat ggcatcgccg atctggccac cggccggcag
     1381 cgcgaacgtg cgtcccgcca cgatggtcac ggtgtcggcc cgcgacgcgc cggtgggact
     1441 gggcaccgtg aacaccgcgc ccgtcacgcc ccggttgagc gccgtcgccc catcgatggc
     1501 gaacgcctgc acgatcacct gggtcgggaa gaccgagatg ctgttaagct tcaaggacag
     1561 cgtgactttc tggtcggtgg tcgtgcccgc gaagttcacc gagtccccgc ccagaggcgc
     1621 cccgagcaga tcggtgacct tgaagcccac gcgggtgatg cccgacgggt cggtggcatg
     1681 gatggtgatg ctgtccgtcg cctcggcgcg cagagcgact gcctgcgaga tctccggcgg
     1741 gacgttgtca gtagctatcg actgaatggt cactgtcacc gcggcgctga ccgcccggcg
     1801 gcccgaggag tccaccgcga agccaacgac ctggaacgtg cctgaggtac cggtcaccag
     1861 gaccgagtcc gagaaaacgg tcgagtcagg gaacggcggc gatccggtgg agatggagtc
     1921 cgcggtcgtc gccgtctgac ctgccgtccc gccgatcgtc caccctaggc ggcggatacc
     1981 ccccgcctgc tggacggcct tcacctgaat cgggatgtat tttccgctgc ctgccacggc
     2041 gcccggcgta ggcgccagca ggaaggccgc gagcgcctgc ttgttggaca agaagatcgt
     2101 gatggtgtcg ggtttggagc ggtttcccgc gccgtcgcgg gcggtgccga cgataaggat
     2161 ctggccgccg gcgccggcga gctgggccga cgtcaggctg aagacgtagg tgacgctctt
     2221 cggcccgccg ccgaacccgc tatcatgcgt ggcgatgtag ccgatgctga aggtcaggtc
     2281 gaccgagtcg accgcgatgt tgtcggaggc agtgacgttg aacgagaggc cacccgagat
     2341 cgtctgactg tcgaccttcg ggtggatctt gtttggcgtc gtcagcgtga tccggggcgc
     2401 gatgttgtca cgcgagaccc cgccggtgtt ggccgccgtt tcgcaggccc cgagcacgat
     2461 cgctccaaag gccacggcgg cgtatagacg cgcgtttcgt cctgcccgca tacgaacccc
     2521 cgcctcagtt cccggcgctg gtggtcgcca ggtcgtcgat gatgtggggc gtgatcagaa
     2581 tcaagagatc gcgccgttcc tcctgctgtg acgtgaaccc gaacagcttg cccagaatgg
     2641 gcaggtccac aaggaacggg attccggact tgcttaccgt gacgttcgtc accgtgagac
     2701 cgccaataac ggccgtttcc ccgtcattga ccaggatctg gttgtccgcc tgttgcgttt
     2761 ggaacgtgaa cccgatgtct accggcgcgg ttttgacact cgagttctcc gcgtggactt
     2821 ccatcaggat ctgacggttc gccgtcacgt ggggcgtcac cttgaggtcg atacccgtct
     2881 gccggaacga cacggtcgcg cgcggcactg tgcttccggt tccgccgccg atggagccgg
     2941 cgtcgatggt ccggaggggc actagatcac cgaccagcac ttcggcggtg tggttgtcgg
     3001 acgtcgtcac cgacggctcg gcctgcaggt cggcgagctc gaccgtctgc aacgcctgca
     3061 cgaagctggt caggttgaag ttgccgaccg cggtcgagaa gatgagcgag agggccggat
     3121 tgatgacttc ctggcccgcg ttgccgagcg ccgacagggc gttgccgccg aggtccacca
     3181 ccgtctggct ggcgggcagg gtcccggtcg cgaccaccgc gtccggaata ccgtcaccgt
     3241 tggtgtcgat cggcttcagt gtggcaggat cggcgcgcgc caccaacgag ttgaagaact
     3301 ggcacggatg cgcgccggtg atcaagcagc ccgagccggt gccgaggtcg tacttcacgc
     3361 ccatgtcctc gacgtcggtg cggtcgacga agatcagctt cgcctgaatc gagacctgcg
     3421 gcgtgcggac gtccagcgcc tgcacgaagg cgacgtcctg ttcgatgcgc gactgaatgt
     3481 ccgtgatcac cagcgcgttc gtggtcgtat cggccacgac ggcgccgcgc ttggagacga
     3541 tcgacgccac cgcgccctgc agggccgtcg acttggcgta gttgatccga accagccggg
     3601 tctcggtcgg aaccgtggaa tcggcccggg cgaggtcggc cttgttcacg acgttgatga
     3661 tgccgccggg caacggctgc caggcgagcg cctgcgactc gagaatcgcc tggaacgcga
     3721 ggtcccacgg ctgattcttg atctgcgccg agatggtgcc cttcacgtcc ttgccgacga
     3781 cgatggtgcg gcccgagaac gccgcgaagc cggcggcgac gtccgcgatg tcggccttgt
     3841 cccactggac cgtgatgcgc ggggcctgcg actgcgcggc ctcgcgggca tgcagggcga
     3901 ggtaccgatc gacggccgcg ggattgctgc tcaccggcgt cgtgacctcg gcggcgggtt
     3961 gcggagccgc ctgggaggac gcgggcgcgc tgctctccac cggaggctgg gccgcgggag
     4021 ccggcgtcgc gttgtactgg ctgctccagg cggcgaacgc ggtctgatcg gcgccgaacg
     4081 agaccgtcac cgatcccgtg gcctggtcga tcttgtaacc cttcgcggca tcgagctcga
     4141 tgaccacgcg gacgacgtcg ggcttgaact gggcgtagcg gacgttcttc acgccgcctc
     4201 gggtttgtcc gtcgtagagg accgacgggc ccgtgagccg ggcgccctgc aggtcgagaa
     4261 cgaggcgcga cggattggtc agcgtgaagt ccgtcacctg gaccgcgccg cggagatcga
     4321 tcaccacgga caccttcccg gccgcgggga gcacgctcac cgcccggact tcaccgtccc
     4381 cgaatccgga ggccggcaac gtaggagccg cggcggccag caggcctgcg gcaagggcag
     4441 cgatgcgctt cattggctct cctccggctt ggcgagcgac agggtttcct gtcgttcgaa
     4501 tccgaattcc tgcacggtaa acgtgacgtc acgcggtctg atctgcgcga ctttcaggcg
     4561 gccgaggacc tcccccggct tcacctggta gcgtttctgc gtggtgatgt cgcggagcac
     4621 cgcgaccgag cggcccggat actggccgtc gaagtagatg gccaggagct tcaagtcggc
     4681 gaagagcgga cgcacgtcgc cgttccgcag gagggacgcg aacggatcgc ggcccgagcc
     4741 ttcgtaggag aagacctcgc gcagcagcgt ggtcgtcgcc gagtccttgg ccgccgcgga
     4801 gggggccgcg gtcttggcgg cagtggtgtc tttcttggcc gcgcccggag ccggcgtctg
     4861 agccgacagg ggcagcgcgg cgagcagaag cacgccgaac atccgagtca tggtcattgc
     4921 gcgccccccg actttgcggg cgcggcggcc gcgggcttct tcgcccccgc cggagccggc
     4981 gccgattgcg ccttcacgta ggtgcggagc gtgagatccg cctcgaggag gccacccagc
     5041 gtgtccccga gcagcttcat cacgttgggg gtcgcgggct tcagactcac gttctggggc
     5101 accacgatgc gcggcatgct tgcgatgtcc gcgaagaact cgccgagctg gtcgtagtga
     5161 ccgtacaccg agagcttgta ctgataggtg tcgaagggag acccgtggtc cacgctcgcg
     5221 ggcgcgaact gctgctgagt caccccccgg atcttcatct tggtggagat gtcgtccagc
     5281 aggttcggga cgtcgttctt ctccgggacc agctgccgca tcagcccgag gtccgcctcg
     5341 tactcgtcgg ccttgcggcg gaggtcttcc accgagccgc tcgcgagatc ggccttcgcc
     5401 ttgttgacca cgtcattcag gctgtcgagc gtcttctgtt gcacgttgat ggcatccgag
     5461 gtcggcgtgt ggacgtaggt ccagaacgcg tacgcgccac ccagagccag gatcaccagg
     5521 atcagcatca tctgggaccg ttggtctttc ggcatcccga gggccatggg ctacctcacc
     5581 gactgcacga gcggaaccgt gcggatgaag acggagtcgg cctgtttgta cttgaatgac
     5641 accgagaact gcgtcacggg gcggtccgac tcgatgacgg tctgggccgc cgccggcgtg
     5701 acgtcggtga tccagggcga cgccgcgagc tgacgcagga acgtcgtgta cgcctgaatg
     5761 tctacggttc ggccgttcag cgtgacgttg acggcattca acgcggccgc cgagtcgacg
     5821 gagctcggct tcgcgccgcc actggacgga ttggagggcg gcagcaccgg cgcactcgcc
     5881 gtttgctgca tgtcctggag ccaggtgtag ggcggcagcg ccttcgtgat ctgatccatc
     5941 aagtgcggcc acacgtaccg ctggccgtcg atttgccgga tcacgttgat ctggaccacc
     6001 agggaatcgc ggtgcaactc gagtttgtgc ttttccgcga tgtacccgct gtagcggtcg
     6061 gcgtcgtgct tcgcggtcgt gaccttgctg tcgagcaggg ccttcttggc ggagtcgagc
     6121 aggaacagcc cgagtgcccc gccgccgatc acgatccagg acaccaccgc ggcgatgagc
     6181 cacggatcct tgacgctggc cagcagggtc ttgaagtcgg ggagtttgaa cttcgccccg
     6241 cctccggcct gcttcttctt ctgtccgggt aggagattga cttcaatcat gcagcacgcc
     6301 tcagcgccag gcccacggag agcatgagca gcggtgcgat ctcgtcgatc gacacggttt
     6361 caaacgcttc ctgacggtac gaaagccgct gaatcgggct cgccagctcc accggcactt
     6421 tgagccgggt cgccagcgct tcgttgatgc cggggatccg cgcgccgcca cccgaggtgt
     6481 agacccgggc cagaccgccg gcggagcggg acgcgctggc caggaaggcc gccgcccgct
     6541 cgacacccac cgagatctct tcgccgcgct gttcgacgta ggaagcgagg tccgcgctct
     6601 ggaccgcgcc cgtaaccgct ttttcggagt cttccgcgga caggcccttt tcgcgctgca
     6661 ggtcttcgcg gaaacgccgg gtgcccacgg agaggtcccg ggtcaggacg gggacgccgt
     6721 cctccaggat gttcacgttg gtcacttcat ggccgatatt gaccaaaccg accaccccat
     6781 tcatcgcgtc aggatggttc agctcgaagg cgttatggat ggcgaaggcg tcgacgtcga
     6841 tgacggatgg ctggagtcct gcctcggtca gaagggagac ccggctttcg acgagctccc
     6901 gcttggccgc cacgagcagg acgttcatct ggagcccctc ggcgttgggg tccaggatct
     6961 ggaaatcgag ctccacgttg gccatgtcga acggcacgtg ctgctcggct tcccagcgga
     7021 tcacctcgcg ggcgtccgct tctttcatcc ggtccatctg gatcttcttg acgatgacgt
     7081 cgcggccgcc gacggcggtt acgacggcct tctgcttgac cccggcggtg gaaaagagac
     7141 cccgaatggc ctccgccacg atgccggggt ccatgacctc gccctcgacg atggcgtcgg
     7201 tcgccacctc ggtggtggca actttgacca gttcaggttc ggacccggaa tggtcgacga
     7261 ccaccagctt gatgatgccg gagccgacgt ccaggccaac cgaggttttt ttgccgccca
     7321 agaagccgaa gagggccata gtcgccttga gttagtgaca gaggggtcaa gggcaaacta
     7381 cagtatggtt caccccttat cctgtcaagc ctcgaagcaa ctttaggcaa atcaaacaag
     7441 ttttggtatc gccgacactt aacccacttg acttagacta gtccatgacc tcgacgggag
     7501 ccgttccggc gttccaaatc ttgataatac gttgctcact aatgctttat catagatcac
     7561 ccacaggctg ctgtcaccag tcgcccccat aagtgtcacg acactgccag ataagtgggt
     7621 gctcaacggg acggctcctg acaccaccag ctccccacgc acgagaacca ccccgttgaa
     7681 tgtgagcggg cccgtcaccg tgagatttcc gtcaaccagg aggaacccgc tcccgctccc
     7741 tccgcccagt tcggcgtccc ccacgatgtg gatgaccccg tccccagtag ccgatggcag
     7801 agtcccatat cgtccacccg gaatcgtcag gtcagccatg gacgcgactc gcgccaggtc
     7861 caggtttccg gtcagccccg tgccggtggc ccccgaatcg atgaggccgt cgaccctcgt
     7921 tcccgtgccg agcgagacga caccgccggc aatcaaggcg ccctgaggaa atgcgaccgg
     7981 tttgagccgt acgagcagcc ccaatccgga acggtcgatc aggtacaggc cttcgctgat
     8041 gcgcgtcgca ttccgagggt cccacgactc gagggcgtgc ccgatcgcca cctccgcgcc
     8101 cccgacctcc tggagctggg agacggaccg ctcggacagc ctggtttcgt taacgagaaa
     8161 aaccgagccc gccgccatta acgcgagcga gacaaccagc atgagcaacg atgagagcaa
     8221 cgccatagga cgctagaccc ggaagaggac gttcataacc ccgggcgaca gcccggggtc
     8281 gagacggcgc tagggtgcgg gattgttcct gagcgtgacc caacggatca gactgtcacc
     8341 caggagcgcg gtcaccccac gccgtgtcac ggg
//
LOCUS       DASUBP010000231         6926 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_879580, whole genome shotgun sequence.
ACCESSION   DASUBP010000231 DASUBP010000000
VERSION     DASUBP010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6926)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6926)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6926
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_879580"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VFU75_08700"
     CDS             complement(<1..956)
                     /locus_tag="VFU75_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466956.1"
                     /translation="MDRIVMRPSLLLLTVLVLCPPGLRAQSSTFIIMQGKDTVSFEQF
                     TRSGNAITGVWIPNNGQVQVHDYVLTLDSAGQPMRYDMAVSFPDGAGHLPPTEAKYIL
                     EFGPDSLTLTTGRTKPVTRRMAMKSGTYPAVGWSVLDRELRLTHLRAAHADSGDFAFT
                     QISGGRAAPAEVVPVRFVGDSAIVGTTHYKVDDAGHILGWRLESGEEARRVPPMDAKK
                     YEDGLIAADAEAEAAARAKLITLPATALDRLVGEYVLNANVTLVVARDSNKLTIRTGP
                     RPAAELMAHSATLFSIGRALSGQMIEFETDSAGTATALTILAP"
     gene            1090..2763
                     /locus_tag="VFU75_08705"
     CDS             1090..2763
                     /locus_tag="VFU75_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-binding protein"
                     /protein_id="HEU5466957.1"
                     /translation="MDIHRLPEGVTADMVRDAHNADMQLQAKYGVNYRGYWFDSVRGT
                     VACLAEGPSSEACVAVHKHAHGLVADTIIEVNSDNVTAFLGGGEIAPTGEAVHGDGTL
                     DAGVRVVVVTELHNLAGVGSRLGDAAALKILQRHDHIVKQAAATHYGREVRHLGDGMM
                     LSFAAASFAVQCALAIQKACAAERTVGDEGPLVRIGMAAGEPVAQHAGLFGVAVDHAR
                     ALARAAQPGEILVSVAVQALCAGKGLAFDAAPPVALSGSDDQLLAAAPRDPSSPPRVT
                     HAGPEDQPRRLRAALAGRYDLEHELGHGGMATVHLARDVRHDRRVAIKVLRSELAAVL
                     GMDRFLQEIRVAAQLTHPHIVALYDSGEADGVLYYVMPSLEGESLRAHIERVGPMAVD
                     RAVEIARSVGGALDYAHRKGVVHRDIKPENILMHEGQPMVLDFGIALAVTQAKGDRMT
                     EPGMALGTPAYMSPEQAVGDPDVDSRADIYALGCVVYEMLAGEPPFTASNVQALIAKI
                     VADKPTRLARHRPDLPSHIEQAVHRALEKAPADRFDSAGAFAAALQPSR"
     gene            complement(3013..3483)
                     /gene="tnpA"
                     /locus_tag="VFU75_08710"
     CDS             complement(3013..3483)
                     /gene="tnpA"
                     /locus_tag="VFU75_08710"
                     /inference="COORDINATES: protein motif:HMM:NF013921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEU5466958.1"
                     /translation="MRHRIYAHLVWTTRDRKCLIDLRVAKFLDRFLRDVARQERALVL
                     EAGIVQNHVHLLIRQHPTTNIPQLLQRLKGGSAIVADQERHATVALRWARGYTIDSIS
                     RHNLADARAYVRDQPGRHPALVIPGWRPADPVSSAREEEWIGENRTWIVPRRRS"
     gene            complement(3692..4057)
                     /locus_tag="VFU75_08715"
     CDS             complement(3692..4057)
                     /locus_tag="VFU75_08715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466959.1"
                     /translation="MNIVLWILQILLAFYGVSGGIWTLRMAPKLGAASTNTLPAPAWS
                     TVAGLQLLFGLGLVVPGATGVMTGLTAIAAVGLAVVMVGSASIVLGKARTSVGMLWAV
                     LPALAALFVAYGRTVLRPF"
     gene            complement(4081..4599)
                     /locus_tag="VFU75_08720"
     CDS             complement(4081..4599)
                     /locus_tag="VFU75_08720"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HEU5466960.1"
                     /translation="MTDRTRYAPGPVSKAEVRKGGEKWTLILVKELRHAPALVWQALV
                     DPAQLREWAPYEVEGDLGKAGSALKLTWVGTGVVTEAKVARVDPPRMLEYADTKWELE
                     AMGGGTRLTLWATIDRRFVSWGAAGWHICFDVLDHLLSGAPIGRLAGGDVMQLGAWQR
                     LRGEYTRQFANE"
     gene            complement(4596..4931)
                     /locus_tag="VFU75_08725"
     CDS             complement(4596..4931)
                     /locus_tag="VFU75_08725"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HEU5466961.1"
                     /translation="MISVFDIIAEPNRRAILTLLASAERTVGEIERRLRMPQPTVSKH
                     LRVLRDAGLVECTVDAQRRVYRVKPDALREADTWLEQFRRFWSGHVDALERHLDRTHP
                     PTRTKGPRT"
     gene            complement(4990..5700)
                     /locus_tag="VFU75_08730"
     CDS             complement(4990..5700)
                     /locus_tag="VFU75_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466962.1"
                     /translation="MKIKLGRVLFAVTIIALGILDLVYRDTLMWKVLPKSLPASTTTV
                     LAIITGVLLVVAGAGLLTPQFQVVASRVLLLFLLLWDVLIGVPPVIQSPGAEVTWLVL
                     GMMTIVLMAGWLLAGKGQVAAARTIVGLALLPVGLSHFSYQQVTLDLVPLWMPARLFV
                     VYGVGAAHAAAGIGLLFGVLPRLAARMEAGMLLAFAVLVWIPRVISNPATHFNWTELL
                     GTVMIGAAMWVVADSWKP"
     gene            complement(5697..6074)
                     /locus_tag="VFU75_08735"
     CDS             complement(5697..6074)
                     /locus_tag="VFU75_08735"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5466963.1"
                     /translation="MSNLYARSVFFVENAERALHFYKEELGFAQDWSYPEGGPAWVCQ
                     VSLLGFELILNQTDEETSDRAGRGRLFIGLDDDQGEPLIKHLASMGVHPERREWGRPT
                     MVIKDPDGNELFFWMPKDEWPGL"
     gene            complement(6071..6778)
                     /locus_tag="VFU75_08740"
     CDS             complement(6071..6778)
                     /locus_tag="VFU75_08740"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HEU5466964.1"
                     /translation="MTVKTLGRPLFAASLIACGAAGVALRNVLVYETFDRSPAHTRIA
                     IVTGVLLVVAGIGLLSRFRVAASLLALVVLLLWDLIYNLPGIVSAPRNEDSWLELGMA
                     TMVVVGAWLLTGTGRIRAARTIFGLALIPVGLSHFFFWKVTLGLVPAWMPAPQLWVAL
                     VGVAHLAAGLGVLFGIVPRLAAMLEASQLILFAFLVWLPRVIAKPSMQFNWTEMLGTL
                     LIGAAAWVMADALPDGR"
BASE COUNT         1236 a         2332 c         2285 g         1073 t
ORIGIN      
        1 ggggcgagga tggtgagtgc ggtcgcggtg cccgcggagt cggtctcgaa ctcgatcatc
       61 tgacccgaca acgcgcgccc gatggagaac agcgtcgcgg aatgcgccat gagctcggca
      121 gccggccggg ggccggttcg gatcgtgagc ttgttgctgt cgcgggccac aacgagagtg
      181 acgttcgcgt tcaatacata ctcgcccacg aggcggtcca gcgcggtcgc cggcagcgtg
      241 atcagcttgg cacgcgcggc ggcctcggcc tcagcgtcgg ccgcgatgag cccatcctca
      301 tacttcttgg catccatggg cggtactcgg cgcgcttctt cgccactctc gaggcgccag
      361 ccgaggatgt gaccggcgtc gtcgaccttg taatgcgtcg ttcccacgat cgctgaatcg
      421 ccgacgaacc ggaccggcac gacctccgcc ggcgcggcgc gcccgccgct gatctgtgtg
      481 aacgcgaagt ctccggagtc ggcatgagcg gcgcgcaggt gcgtgagcct cagctcccgg
      541 tccagtacgg accacccgac cgccgggtag gtcccgctct tcatcgccat ccgccgggtc
      601 accggcttgg ttcgacccgt cgtcaacgtg aggctgtcag ggccgaactc caggatgtac
      661 ttcgcctcag tcggtggcag gtgcccggcg ccatcaggga aactcaccgc catgtcgtat
      721 cgcatgggct gcccagctga atccagcgtc agcacgtagt cgtgcacctg cacctgaccg
      781 ttgttgggga tccagacgcc ggtgatcgca ttgccggagc gggtgaactg ttcgaacgaa
      841 accgtgtcct tgccttgcat gatgatgaac gtggaggact gtgcgcggag acctggggga
      901 cacaagacga gaacggtgag caacaacagg gatggccgca tgacgatccg atccatgggt
      961 ggggaacgca ccctacgtgc agaatgcacc ttcgtttcac cctcagcatt ccgacccgag
     1021 aagctccctc cacgttccac tttgcgccat acggcaagaa cggctacgtt cgaccccatg
     1081 cctcttttca tggatatcca ccgcctgccc gaaggcgtga cggcggacat ggtccgcgat
     1141 gcccacaacg ccgacatgca gctccaggcg aaatacggcg tcaactatcg cggctattgg
     1201 ttcgacagtg tccggggaac cgtcgcgtgt ctcgcggagg gaccctcctc cgaggcctgc
     1261 gtcgcggtgc acaaacacgc ccacgggctc gtcgcggaca cgatcatcga ggtgaactcc
     1321 gacaacgtga ccgcctttct cggcggcggg gaaatcgcgc cgacgggaga agccgtgcat
     1381 ggcgacggga cgctcgacgc gggcgtccgc gtggtcgtcg tgacggagct ccacaacctg
     1441 gcgggcgtgg ggagccggct cggggacgcc gcggctctga agatcctcca gcgtcacgac
     1501 catatcgtca agcaagccgc cgccacccac tacggccgcg aagtgcggca cctcggcgat
     1561 ggcatgatgc tgagcttcgc cgccgcgtcg ttcgccgtcc agtgcgccct cgcgattcag
     1621 aaggcctgcg ccgcggagag aaccgtgggg gacgagggcc ccttggtgag gatcgggatg
     1681 gcggccggtg agccggtcgc tcagcacgcc ggcctcttcg gagtggcggt cgatcacgcg
     1741 cgggcgctcg cccgcgccgc gcagcccggc gagatcctgg tctccgtcgc agtgcaggcg
     1801 ctctgcgccg ggaagggatt ggcgttcgat gccgcgcccc ccgtcgcctt gtccggctcc
     1861 gatgaccagc tcctggccgc cgcaccgcgc gacccgtcat ctcccccgcg ggtcacacac
     1921 gcaggtcccg aggaccagcc gcgccggctc cgcgcggcgc tggccggccg gtacgacctc
     1981 gagcatgagc tggggcacgg gggaatggcc accgtccatc tcgcccgcga cgtgcggcat
     2041 gaccggcgcg ttgccatcaa agtgctccgc tccgagctcg cggcggtcct cggcatggac
     2101 cggttcctcc aggagatccg ggtcgccgcg cagctgaccc acccccacat cgtggcgctc
     2161 tacgattccg gagaggccga cggcgtcctg tactacgtca tgcccagcct cgaaggggag
     2221 tcgctgcgcg cccacatcga gcgcgtgggc cccatggccg tggaccgcgc cgtggagatc
     2281 gcgcgaagcg tcggcggcgc gctcgactac gcccatcgca aaggggtcgt ccaccgtgac
     2341 atcaagcccg agaatattct gatgcacgag ggacagccga tggtgctcga cttcgggatc
     2401 gccctcgcgg tcactcaagc gaagggcgac cggatgacgg aaccgggcat ggcgctgggg
     2461 accccggcct acatgagccc cgagcaggcg gtcggcgacc ctgacgtcga cagtcgtgcc
     2521 gacatctacg ccctcggctg cgtggtgtac gagatgctgg cgggcgagcc gcccttcacc
     2581 gcgtccaatg tccaggcgct gatcgccaag atcgtggccg acaagccgac ccggctggcg
     2641 cggcaccgtc ccgacctgcc gtcacatatt gaacaagcgg tgcatcgtgc cctcgagaag
     2701 gctccggccg accggttcga ttcggcgggt gcgttcgccg cggcgctgca gccttcccgg
     2761 tagtccgtca gggcgccgct atcatgcggc agacgccgag ccgcggcttt acgccgcgcc
     2821 ggcaacaccg tgactggggc ctggtctccc aagaatcggc ctccaaaggc tcgaatacga
     2881 aacgcccgcc tgacgggtca ccgcaggcgg gcgttcgtgc gggattgttg ctggtccgac
     2941 cgtcgaagtg ccggcgcggc gtaaagccgc ggctcggcac ctgcccgatg agacgggcct
     3001 gcgcgccgat catcaggaac gacgccgggg gacgatccac gtccgatttt cgccgatcca
     3061 ctcttcctca cgcgcgctcg agactggatc agcgggtctc caacccggga tgaccaatgc
     3121 cggatgtcgg cccggctgat cacgaacata cgcccgagca tcggccaggt tgtgccggct
     3181 tatgctgtcg atggtataac cccgagccca tcggagggcg accgtcgcgt gacgctcctg
     3241 gtccgccacg atcgcgctgc cccccttcag tcgctgcaga agctggggaa tgttcgtggt
     3301 gggatgttgt cggatgagga ggtgaacgtg gttctgaacg atcccggcct cgagcacgag
     3361 cgctcgctcc tgacgggcca cgtctctgag gaatcgatcc aggaacttgg cgactcgcag
     3421 gtcgatgagg cacttccggt ctctcgtggt ccacacgaga tgcgcgtaga tccggtgacg
     3481 catggcgaca cgttgtggcg agacccacaa ttgacggtca actgggcgcc gctatcatgc
     3541 ggcagacgcc gagccgcggc tttacgccgc gccggcaaca ccgtggctgg ggccggtctc
     3601 ccaggaatcg gcctccaaag gctcgaatac gaaacgcccg cctgacgagt caccgcaggc
     3661 gggcgtcgag cgtttgggcg caggatccgg gtcaaaacgg ccgcaacacg gttcgtccgt
     3721 acgccacgaa gagggctgcg agggcaggga gcaccgccca gagcatgccc accgacgtgc
     3781 gagccttgcc gagcacgatc gaggcagagc cgaccatcac gacggccagg cccaccgccg
     3841 cgatggcggt aagtccggtc atcacgccgg tcgcccccgg cacgacgagc ccaagtccaa
     3901 acagcagctg cagccccgcg acggtcgacc aggccggggc agggagagtg ttcgtcgacg
     3961 ccgcgcccaa cttcggggcc atgcgcaggg tccagatgcc gccactcacg ccatagaaag
     4021 ccagaaggat ctgaagaatc cagagtacga tgttcatgtt cccctcaggt gttggtgtgg
     4081 ttactcgttt gcgaactgcc tggtgtattc gccgcgcagg cgctgccagg cgccaagctg
     4141 catgacgtct ccgccggcga gccggccgat cggagcgccc gagagcaggt gatccagcac
     4201 gtcgaaacag atgtgccatc cggccgcgcc ccaggagacg aaccggcggt cgatggtggc
     4261 ccacagcgtc aggcgggtgc cgccgcccat cgcttcgagc tcccacttcg tgtcggcgta
     4321 ctcgagcatc ctggggggat cgacccgcgc gaccttcgcc tcggtgacca cgccggtccc
     4381 cacccaggtg agcttgagcg ccgagccggc ctttcccagg tcgccttcga cttcataggg
     4441 agcccattcg cggagctgag cgggatcgac cagcgcctgc cagaccagcg ccggcgcgtg
     4501 gcggagctcc ttcacgagga tgagcgtcca tttctcgccc cccttgcgga cctccgcctt
     4561 gctgacgggg cccggtgcgt atcgcgtgcg atcggtcatg tgcgtggccc cttcgttcgt
     4621 gtgggcggat gcgtccggtc gagatgacgc tcgagggcgt ccacgtgacc ggaccagaaa
     4681 cggcggaact gttcgagcca ggtatccgct tcccgcaacg catccggctt gacccggtag
     4741 acccgccgct gcgcatcgac cgtgcattcc accagtcccg cgtcccggag cacccgcagg
     4801 tgcttggaca cggtgggctg gggcatccgc aaccggcgtt cgatctcccc gaccgtccgt
     4861 tcggcggacg ccaggagggt caggatcgcc cggcggttgg gctcggcgat gatgtcgaag
     4921 acggatatca tggacctaat atgcgtatag cagtatatac ttgtcaaggc atataaaaag
     4981 cccgacaaat cacggtttcc aggaatcagc caccacccac atcgcggcgc cgatcatgac
     5041 agtcccgagc agctcggtcc agttgaaatg cgttgccggg ttactgataa ccctggggat
     5101 ccagaccagc accgcgaacg ccagcagcat ccccgcctcc atcctggccg ccaggcgggg
     5161 gaggacgccg aacaacaggc caatccccgc ggctgcgtgc gcggcaccga ccccgtagac
     5221 cacgaacagc cgcgccggca tccagagggg gacgagatcg agcgtgactt gctgatacga
     5281 gaagtgagag aggccgaccg ggaggagcgc caggcccacg atggtgcggg cggcggcgac
     5341 ctgccccttg cccgccagaa gccaaccggc catcagcacg atcgtcatca tcccgaggac
     5401 cagccaggtg acctcggctc ccggtgattg gatgaccggt ggcactccga tcaggacatc
     5461 ccataggagc aggaagagga gcagcacgcg ggaggctacc acctggaatt gcggtgtgag
     5521 cagaccggct cctgcgacga ccagcaacac cccggtgatg atggcaagga cggttgtcgt
     5581 cgacgcgggg aggctcttgg ggagcacctt ccacatcaac gtgtcccggt acacgaggtc
     5641 caggatgccc agcgcgatga tcgttaccgc gaagagcacg cgtcccagtt tgatcttcat
     5701 agtcccggcc actcgtcttt gggcatccag aagaacagct cgttgccgtc ggggtctttg
     5761 atcaccatgg tggggcgtcc ccattcccgc cgctccgggt gcactcccat ggacgccaga
     5821 tgcttgatca agggctcgcc ctggtcgtcg tcgaggccga tgaacaaccg gccacgtccc
     5881 gcccggtcgc tcgtttcctc atccgtctga ttcagaatca attcgaagcc gagaaggctg
     5941 acctggcaca cccatgctgg cccgccctcc ggatagctcc aatcctgggc gaagccgagc
     6001 tcttccttgt agaagtgcag ggcccgttcc gcgttctcca cgaagaagac gcttctggcg
     6061 tagaggttgc tcaccgcccg tccggcaggg catcggccat gacccacgcc gccgccccga
     6121 tgaggagagt ccccagcatc tcggtccagt tgaactgcat ggagggcttc gcgatcaccc
     6181 gcggcagcca gacgagaaac gcgaacagga tcagctgact cgcctccagc atcgcggcca
     6241 gtcttggcac gatgccgaag agcacgccga ggccggccgc gaggtgggcg acgccgacga
     6301 gggcgaccca cagttggggc gccggcatcc aggccggaac caggcccagc gtgaccttcc
     6361 agaaaaagaa atgcgagagt cccacgggga tcagggcgag gccgaagatc gttcgcgcgg
     6421 cgcggatccg cccggtcccg gtcaggagcc aggcacccac gacgaccatg gtcgccatcc
     6481 ccagctccag ccaggaatcc tcattccgcg gcgcgctcac gatcccgggg aggttgtaga
     6541 tgaggtccca gagaaggagc acgaccagcg ccaggaggga ggcggcgact cggaatcggg
     6601 acaggagtcc gatgccggcg acgacgagga ggacgccggt gacgatggcg atccgcgtgt
     6661 gtgcgggcga acggtcgaat gtctcgtaca cgagaacgtt gcggagcgcg acaccggcag
     6721 cgccgcacgc aatcagactt gccgcgaaca gtgggcggcc aagcgtcttg acggtcatcg
     6781 gacgaaagta gggtcctcag gtaccaccgg taaggcggag caccgcgcca tttggcgcgc
     6841 caaacggcgg ttacgtcgcg gtggaagccg ttcgccggcg ccactcgatc agcgcgatga
     6901 gcaccagcgc gggcaggcac acgaac
//
LOCUS       DASUBP010000232         4990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_307225, whole genome shotgun sequence.
ACCESSION   DASUBP010000232 DASUBP010000000
VERSION     DASUBP010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4990
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307225"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1662
                     /locus_tag="VFU75_08745"
     CDS             <1..1662
                     /locus_tag="VFU75_08745"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5466965.1"
                     /translation="DAASGLVREALTVEGPDAFALADSAAARFAARIGRPKPTPGLES
                     VTSPSLLAHDLYNQGLRTYYRDGDYTAAARLFEDALAQDSTFAMASYYLGRSREALDP
                     SAGASDYARAVRLSAHATEQERLLIQVQWPAAQNEPRWVVLAESLGARNAADPGARYA
                     EGSARLMTGEYSRAASSFREVIRLDSLGLRYPDARCMACDAYERLVQTDIARDSMDQA
                     LRDAGAWLKSQPQAPRAWDLWADALERQERLAEALAARDTAARLRSGSGATPVDTAYF
                     LIRGGNPDSATRILARAEHRGSTKDREDALWWRAIALRYAGRPREALREGRAILQQDA
                     AGPDGLGLSGTPLGVSFLEAGDLAAAARIFDSAGVHSAAYRREHPGLAARHRAWGLAQ
                     RATVAAAQNDVPALRMLAESIQVDAAQSAYGRDHTLPTYVRGLILARTGQRRAAADSL
                     RAAIFSPTEGYTRVSSELARILLVQGRPGEAIPWLQSALRGGIEASNYYLTQTDLHEL
                     LAQCFAAAGMADSARAHAAWVTRAWERAEPEFAARKQLMERLAAP"
     gene            1679..2590
                     /gene="xerD"
                     /locus_tag="VFU75_08750"
     CDS             1679..2590
                     /gene="xerD"
                     /locus_tag="VFU75_08750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEU5466966.1"
                     /translation="MSDAPPVPPAFLVEPFRDYLALEAGNSPHTVDNYLRDIRRFARY
                     SMAAGVTGPADVTGARVRDFVYSLKDVGLTPATIRRQISALRTYYRFLIGEGKVVQDP
                     TARIETPKRWRTLPAVLSVEEVDKLLSAPDTDAELAIRDRALLEFGYATGARVSELVG
                     LKLQDIMFEDGIARLFGKGSKERLVPVGRRAMGQVALYAREIRPTLDRGKGSGRLFLN
                     AHGTPLSRVGAWGIIKKAAKRAGITKRVTPHTLRHTFATHLLEGGADLRAVQEMLGHA
                     DLATTQIYTHVDRDYLRTVHRQYHPRS"
     gene            2597..3028
                     /locus_tag="VFU75_08755"
     CDS             2597..3028
                     /locus_tag="VFU75_08755"
                     /inference="COORDINATES: protein motif:HMM:NF020397.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEU5466967.1"
                     /translation="MPTVAQFLKSLPPERRREVTLVRDAIRKRMPKGYEEVVEGKMIV
                     YQVPIVHAPATYNGHPLWYAALAAPKSYLTLHLMPVYSSPALLDQLKRGFEASGKHLD
                     IGKACIRYRRAEDLDLKTIGDVVAGVPMAKWIALANASRKR"
     gene            3025..4290
                     /locus_tag="VFU75_08760"
     CDS             3025..4290
                     /locus_tag="VFU75_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU5466968.1"
                     /translation="MSDVPQSIEAVWRIEAAKVIAVLTRLMGDVGLAEDLAQEALLAA
                     LEHWPQSGIPDNPGAWLITTAKHRGLDILRREHRIEDKHREIEVDLARRPDEALAKLD
                     DVIDGEVEDDLLRLMFISCHPVLAPENRVALTLRLLGGLTTEEIARGFLVPTPTIAQR
                     IVRAKRTLKEARVPFESPRGEERDARIASVLEVLYLIFNEGYSATAGDDWVRPALCED
                     AMRLGRVLAELAPQDPEVHGLVALMEIQASRLKARVDATGAPVLLLDQDRTRWDRALI
                     QHGLESLGRAELGGTLGPYTLQAAIAACHARAATPEDTDWTRITALYDALVQLMPSPI
                     VELNRAVAYGMAFGPAAGLEIVEPLRRDPALARYHLLPSVVGDLLHRLGRFEEARAEF
                     ARAATLTSNATEQALLRARASTMTGQGGS"
     gene            4298..4909
                     /locus_tag="VFU75_08765"
     CDS             4298..4909
                     /locus_tag="VFU75_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5466969.1"
                     /translation="MPTSTTAPSVLIIDDHSPDARLTLDALQDGADGHHFIAKDAREA
                     LAYLRKEAPFAEVPTPDVIVLGFNPPARTGREAFEGIRTLDLHPPIIILSRTMEEPVS
                     RYLHAGAADFLVKDDLRPLRQAVDAVVATRAPLRKLSPRQIEVLRLIAGGLATREIAQ
                     RLEVSGKTVEAHRAELMRRLGIRTVAGLVRYALRASLVELGRE"
BASE COUNT          830 a         1728 c         1633 g          799 t
ORIGIN      
        1 gacgcggctt ccggactggt gcgggaggcc ttgacggttg agggaccgga cgccttcgct
       61 ctcgccgact cggcggcggc gcggttcgcg gcgcgaatcg ggcgacccaa acccaccccg
      121 ggcctcgagt cggtgacctc accatccttg ttggcccacg atctctacaa ccagggcctg
      181 cggacctatt accgggacgg ggattacaca gccgcggcac gcctattcga ggacgccctg
      241 gctcaggata gcacgttcgc catggcgtcc tactacctcg gccgctcccg cgaagcactg
      301 gacccgtcgg ccggcgccag cgactacgcc cgtgccgtcc ggctctcagc acacgccacc
      361 gaacaggagc gactccttat tcaggtgcag tggccggcgg cgcagaacga gccgcgctgg
      421 gtcgtgctgg cggagtcgct cggtgcccgc aatgccgccg atcccggcgc ccggtacgcg
      481 gaggggtcgg cgcgcctgat gaccggagag tacagtcggg cggcctcgag cttccgcgaa
      541 gtaatccgac tcgactccct gggtctccgc tacccggacg cgcgttgcat ggcctgcgac
      601 gcctatgagc gtctggtcca aaccgatatc gcccgcgact cgatggacca agccctgcgc
      661 gatgccggcg cctggctcaa gagccagccc caggcccccc gagcctggga tctctgggcc
      721 gacgccctcg agcggcaaga gcgcttggcc gaggccctcg cggcacgcga caccgccgcg
      781 cgcttgcgca gcgggtcggg cgccacaccc gtggacacag cgtacttcct gatccgcggc
      841 ggaaaccccg actcagccac caggatcctc gcgcgggccg agcaccgcgg atccaccaag
      901 gaccgggagg atgccctctg gtggcgtgcc atcgccctgc ggtacgcggg ccgcccacgt
      961 gaggccttgc gagagggacg tgccatcctt caacaggatg ccgccggacc cgatggcctc
     1021 ggactctccg gcacgccgct cggcgtgagt ttcctcgagg ccggagacct ggcggccgcc
     1081 gcacgtatct tcgattccgc cggcgtgcat tcggccgcct accggcgtga gcatcccggg
     1141 ctggcagcac gtcaccgggc ctggggattg gcccagcgag cgacggtcgc ggcggcccag
     1201 aacgacgttc cggcgttgcg catgctggcc gagagcattc aggtagatgc ggcgcagagc
     1261 gcctatgggc gtgaccacac ccttccgacc tatgtccgag gcctgatcct cgcgcgcacc
     1321 ggacagcgaa gggccgcggc ggactcgctg cgggcggcga tcttctcccc cacggaaggc
     1381 tacacccgcg tcagctcgga gcttgcgcgg atcctgctcg tccaggggcg ccctggtgag
     1441 gctatcccct ggttgcagag cgcactgagg ggcggaatcg aagcgtcgaa ctattatctc
     1501 acgcagaccg accttcacga gcttctagcc cagtgcttcg cggcggcggg aatggcggat
     1561 tccgcgcggg cacacgccgc ctgggtcaca cgcgcctggg agcgtgcgga gcctgagttc
     1621 gccgcccgca agcagctcat ggagcggctc gccgcgccgt aagtatcttg gctgacgcat
     1681 gtcagacgcg ccgcccgtgc cacccgcgtt cctggtagaa cccttccgcg attacctcgc
     1741 cctggaagcg ggcaacagcc cgcataccgt ggacaactac cttcgggaca tcaggcgctt
     1801 cgcccggtat tcgatggcgg ccggtgtaac ggggccagcc gacgtcacgg gggcccgggt
     1861 ccgggatttc gtctactccc tgaaggacgt cggactgacg cccgccacca tccgccgtca
     1921 gatctcggcg ctccgcacct actaccggtt tctgatcggc gaagggaagg tcgttcagga
     1981 ccccacggcc cggatcgaga ctcccaagcg gtggcgcacg ctccccgccg tcctctccgt
     2041 ggaagaagtg gacaagcttc tctcggcccc ggataccgat gcagagctcg ccatccggga
     2101 ccgcgcactc ctcgagttcg ggtacgccac cggcgcgcgg gtgtccgagc tcgtggggct
     2161 caagcttcag gacatcatgt tcgaagacgg aattgcccgg ctgttcggca agggctccaa
     2221 ggagcgattg gtgcccgtcg gcaggcgagc catggggcag gtcgctctgt atgcccgcga
     2281 gattcgcccc acgctcgatc gagggaaggg gagcgggcgc ctttttctca acgcccacgg
     2341 cacgccgctc tcccgggtcg gggcctgggg aatcatcaag aaggccgcca agcgcgcggg
     2401 gatcacgaag cgcgtcaccc cccacacgct ccgtcatacc tttgcgactc atctgctcga
     2461 ggggggcgcc gacctccgcg cggtgcagga gatgctgggg catgccgacc tcgcgaccac
     2521 ccagatctac acgcacgtcg atcgagacta tcttcgaacg gtgcaccggc aataccaccc
     2581 gaggagctag acggacatgc ccaccgtcgc gcagttcctg aagtccctcc cccccgaacg
     2641 gcgtcgtgaa gtcacgctcg tccgtgacgc aatcaggaag cgcatgccaa aggggtacga
     2701 ggaagtggtc gagggcaaga tgatcgtgta tcaggtcccg atagtccacg ccccggccac
     2761 atacaacggt catccgctgt ggtacgcggc actcgcggct cccaagagct acctcacgct
     2821 gcatctcatg cctgtgtact cgagtccggc cttgctggat cagctgaagc gggggtttga
     2881 ggcatccggc aagcacctcg acatcgggaa ggcgtgcatc cggtatcggc gggccgaaga
     2941 cttggacctg aagacgatcg gcgatgtggt ggccggtgtt cccatggcga agtggatcgc
     3001 cctggcgaac gcctcccgca agcgatgagc gacgtcccgc agagcatcga agccgtttgg
     3061 cgcatcgaag ccgccaaagt gatcgccgtg ctcactcgcc tgatgggaga tgtcggcctg
     3121 gccgaggatc tggcgcagga agccctgctc gcggcgctcg agcactggcc ccagtcgggg
     3181 attccggaca accccggcgc ctggctgatc accaccgcca agcatcgcgg actcgatatc
     3241 ctccggcgtg agcaccggat cgaagacaag catcgcgaga tcgaggtgga cctcgcccgg
     3301 cggcccgacg aggcgttggc caagctcgac gacgtgatcg acggggaagt cgaagacgac
     3361 ctgctgcggc tcatgttcat ctcgtgtcat ccggtgctcg cgccggagaa ccgtgtcgcc
     3421 ctgacgctgc ggctcctggg cggcctgacc accgaagaaa tcgcccgcgg cttcctggta
     3481 ccgacgccga ccatcgcgca acgcatcgtg cgtgccaaac gcaccctcaa ggaagcccgc
     3541 gttccattcg aatccccccg cggcgaagag cgggacgccc ggatcgcgtc cgtactcgaa
     3601 gtgctctacc tcatcttcaa tgaggggtac tcagccacgg ctggtgacga ctgggtccgc
     3661 ccggcgctct gcgaggacgc catgcgactg ggccgcgtgt tggccgagct ggcgccgcag
     3721 gaccccgaag tacacggcct ggttgccctg atggaaatcc aggcttcccg cctcaaagcc
     3781 cgggtcgatg cgaccggcgc gcccgttctc cttctcgacc aggatcgcac gcggtgggac
     3841 cgcgcactga ttcaacatgg actggaatcg ctggggcgag cggagctcgg gggaacgctg
     3901 gggccgtaca cactgcaggc ggcgattgcc gcgtgtcacg cgcgcgccgc tacgcctgag
     3961 gataccgatt ggacccgtat caccgcgttg tatgacgcgc tcgtacaact catgccgtct
     4021 ccgatcgtcg agctcaaccg cgcggtcgcg tacggcatgg cgttcggccc cgcggcgggc
     4081 ctcgagatcg tcgaacccct gcgtcgcgac cctgcgctcg cgcgatatca tctgctcccg
     4141 agtgtggtcg gggatctcct gcaccgtctc gggcggttcg aggaagcccg tgcggaattt
     4201 gcccgggcgg cgacgcttac gagcaacgcc accgagcagg ccctgttgcg cgcgcgcgcc
     4261 tcgacaatga ctggccaagg gggtagctag agagaagatg ccgacctcaa cgaccgcgcc
     4321 cagcgttttg atcattgacg atcactcgcc agacgctcgc ctcacgctcg acgctctgca
     4381 ggacggggcg gacggccatc acttcattgc gaaggatgcg cgcgaggcac tggcatactt
     4441 gcgcaaagag gcgcctttcg ccgaagtccc aacaccggat gtcatcgttc tggggttcaa
     4501 cccgccggcg cgcaccggac gggaggcttt cgaaggcatc cggactctcg atctgcatcc
     4561 cccgatcatc attctgagcc gcaccatgga agagccggtg agccggtacc tgcacgccgg
     4621 cgccgccgat ttcctggtga aagacgacct gcgccctctg cggcaggccg tcgacgcagt
     4681 cgtcgcgacc cgcgcgcccc tgcgcaaact cagtccccgc caaattgagg tcctgcgctt
     4741 gatcgccggt ggcctcgcta cccgggagat cgcgcagcgg ctcgaggtga gcggcaagac
     4801 ggtggaagcc catcgcgccg agctgatgcg ccgcctgggg atccggacgg tcgcgggcct
     4861 ggtgcggtac gcgctcaggg caagtctggt cgagctgggc cgcgagtagc ccggccctgg
     4921 tcttcatgat cgtcgcctgt cggattctgg tccggcaggc gattcttctt ggtgtgtcaa
     4981 ttccgtatac
//
LOCUS       DASUBP010000233         3530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_634485, whole genome shotgun sequence.
ACCESSION   DASUBP010000233 DASUBP010000000
VERSION     DASUBP010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634485"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..807)
                     /locus_tag="VFU75_08770"
     CDS             complement(<1..807)
                     /locus_tag="VFU75_08770"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HEU5466970.1"
                     /translation="MPRIAIAQFRPKKGDYAANVARIGAILADVAKLEPKIDLVITPE
                     TSTSGYFLEGGVRDVAVTAGTLFQDLVAVHQGPAIDVAVGFYEIFRNRFHNSCLYATL
                     DGKKSEVRHVHRKIFLPTYGVFDEERYVERGNEGVHAFDTGWGGRAAVLICEDAWHSI
                     AGTIAALDGAQLIIVPSASPARGSGVDEEGTRLPASMVRWEQVVRGMAGEHGVYVALA
                     SLVGFEGGKGFPGGSVLVGPSGEIVSRAPLFDEGLMVADVDLDQITHVRAD"
     gene            870..1244
                     /gene="crcB"
                     /locus_tag="VFU75_08775"
     CDS             870..1244
                     /gene="crcB"
                     /locus_tag="VFU75_08775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEU5466971.1"
                     /translation="MILYIVIGGVIGTLGRWYLQGWIQTTSHAGSFPLGTLFINLAGS
                     FVLGFITKLGTGSTLISPEIRGGITVGLCGAFTTMSTFSYESVKLLGDGDYVRAGVYM
                     AGTIIGCLTAVVFGSLAAGRLL"
     gene            1241..1588
                     /locus_tag="VFU75_08780"
     CDS             1241..1588
                     /locus_tag="VFU75_08780"
                     /inference="COORDINATES: protein motif:HMM:NF014677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF190 domain-containing protein"
                     /protein_id="HEU5466972.1"
                     /translation="MTQKFAGERTLMRVFIGESDKYHGRPLYEALVDLFRKKGLAGAT
                     VLRGVAGYGASSTIHTDKVLRLSLDLPIIIELVETQEAIDAVLPELDAMIGGGLITLE
                     KARVILYRGEKKP"
     gene            complement(1644..3029)
                     /locus_tag="VFU75_08785"
     CDS             complement(1644..3029)
                     /locus_tag="VFU75_08785"
                     /inference="COORDINATES: protein motif:HMM:NF025684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4384 domain-containing protein"
                     /protein_id="HEU5466973.1"
                     /translation="MLLTALAFVLNAPMVAPKPAPAFKASDDPPIRVWLNDDNYQIGD
                     RATVKFKLSDDGYVIVLRADTRGHLRVLFPLDPGADNFIKGGKTFEIRARGDRDAFQV
                     DDQSGTGTIFAAVSDQPFAFNNYVLGDHWDYKALDNEDLGNDPEAAMVDLVHGMAGSN
                     HYQYDVTTYTVSQQVAYSDGGGYYAPTYYPTYYPCWGCGPWYGGSGVFINFGFGFRHP
                     YYGYGFYDNCFYDPFFCDGFGVPYYGGYYYGAYYGRPYYGYGSYPRYAGFHPGVYRPF
                     AGTFHAPFSAPRTPVVTGVGVRNRWAGSGTPVARGGRSAPSGFEGGRRGGSFGGAAEP
                     RSRPTPPAARPSSPPPDRGGRNAPEPRRGRGNNDQGFSRPSQPIRQEPARPAPRMESP
                     RYESRPAPRMESRPAPRMESRPAPRMESRPAPQSRSFSMPSFSRGSSAPAPRGGGGGG
                     GGGGGGGRRPR"
     gene            3223..>3530
                     /locus_tag="VFU75_08790"
     CDS             3223..>3530
                     /locus_tag="VFU75_08790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEU5466974.1"
                     /translation="MAHLAALKKAGCQVLLDLREAMEERPYRVPDAVVGAGLEYVNVP
                     VGHGAQPDETFTKVREQVRNLVGKKPTFCCCNSGNRVGAALLPYLMLDRGMDEDEATM
                     V"
BASE COUNT          632 a         1125 c         1194 g          579 t
ORIGIN      
        1 gtcggcccga acgtgggtga tctgatcgag atccacgtcg gcgaccatca ggccttcatc
       61 gaagagcggc gcgcgggaca cgatctcgcc cgacggtccg accaggactg agccgccggg
      121 gaatcctttc ccaccctcga agcccacgag cgacgcgagc gccacgtaga caccgtgctc
      181 accggccatg ccgcgcacca cctgctccca gcgcaccatg ctcgccggga ggcgcgtccc
      241 ctcctcgtcg actccactcc cccgcgccgg cgacgcgctc ggcacgatga tcagctgcgc
      301 gccatcgagc gcggcgatcg tgccggcgat cgagtgccag gcgtcctcgc agatcagcac
      361 cgccgcccgg cccccccaac ccgtatcgaa ggcgtgcaca ccctcgttcc cgcgttcgac
      421 gtagcgctcc tcgtcgaaga cgccgtaggt gggcaggaag atcttgcggt ggacgtggcg
      481 gacttcggac tttttgccgt cgagcgtggc gtagaggcag gaattgtgga agcgattgcg
      541 gaagatctcg tagaacccga cggcgacgtc gatcgcgggt ccctggtgaa cggccaccag
      601 atcctggaac agggtcccgg cggtcacggc cacatcccgg actcccccct ccaggaaata
      661 gccggaggtc gacgtctcgg gggtgatgac caggtcgatc ttgggttcga gcttggcgac
      721 gtcagcgaga atcgccccaa tgcgcgccac gttggcggca tagtcgccct tcttgggccg
      781 gaactgggcg atcgcgattc ttggcatgaa gaaaagttag tcggtcaaac gactaggcgg
      841 ttagacggct agacggctag aatagcctaa tgattctcta catcgtgatc ggcggcgtca
      901 tcggcacgct ggggcgctgg tacctccagg gctggatcca gaccacgagc cacgcggggt
      961 cctttccact cggcaccctc ttcatcaacc tcgccgggtc attcgtcctg ggcttcatca
     1021 cgaagctggg aaccggcagc accctcattt ctccggaaat ccgcggcggc atcacggtcg
     1081 gactctgcgg cgcgttcacg accatgagca cgttctccta cgagagcgtc aagctgctcg
     1141 gcgacggaga ctacgtgcgc gccggggtgt acatggccgg caccatcatc ggctgcctca
     1201 cggcagtggt gttcggttcc ctggcggctg ggaggctgct atgacccaga agttcgcagg
     1261 cgagcggacg ctgatgcgcg tcttcatcgg tgagagcgac aagtaccatg gccggcccct
     1321 gtacgaggcg ctggtcgacc tcttccggaa aaagggcctc gccggggcga cggtgcttcg
     1381 cggcgttgcc ggctacggcg cgtcgagcac cattcacacc gacaaggtgc tccggctctc
     1441 gctcgacctc ccgatcatca tcgagctggt cgagacgcag gaagcgatcg acgccgtgct
     1501 gccggaactt gatgcgatga tcggtggagg gttgatcacg ctcgagaagg cgagggtgat
     1561 tttgtatcgg ggggagaaga agccataagc cgtcggcagg cacgaaaagg gggccccgtt
     1621 gtggagcccc ctttggtcat cgcctagcgc ggcctgcgcc cgccaccacc accaccgccg
     1681 ccgcccccac ccccacccct cggcgcaggg gccgaagacc cacgcgagaa tgacggcatg
     1741 gagaacgagc gcgactgcgg cgccgggcgc gattccatgc gcggtgcggg acgcgattcc
     1801 atgcgcgggg ccggacgcga ttccatccgc ggggccggac gagactcgta cctcggcgac
     1861 tccatccggg gcgccggacg cgccggctcc tggcgaatcg gctgactggg ccggctgaag
     1921 ccctggtcgt tgttgccgcg gccgcgacga ggctcgggcg cgttgcgacc accgcgatcg
     1981 gggggcggcg agctcgggcg agccgccggc ggggtcggac gcgaacgggg ttcagcggct
     2041 ccgccgaacg aaccaccgcg acgcccaccc tcgaagccgg acggagctga ccggccaccg
     2101 cgcgcgaccg gcgtgccgct accggcccag cgattccgca cgccgacacc cgtcacgacc
     2161 ggggtccgcg gcgcgctgaa cggagcgtgg aacgtccccg cgaacgggcg atagacgccg
     2221 gggtggaatc ccgcgtaccg cggataggaa ccgtagccgt agtaggggcg accgtagtag
     2281 gcgccatagt agtagccacc gtagtacggc acgccgaacc cgtcacagaa gaacggatca
     2341 tagaagcagt tgtcgtagaa gccgtagccg tagtacgggt gccggaaccc gaaaccgaaa
     2401 ttgatgaaca cgccggaccc gccataccac gggccgcagc cccagcacgg gtagtaggtc
     2461 gggtagtacg tcggggcgta gtagccgccg ccatccgaat acgcgacctg ctgggagacc
     2521 gtgtaggtgg tcacgtcgta ctggtagtga ttcgagcccg ccatgccgtg cacgagatcg
     2581 accatcgccg cttcgggatc gttgccgagg tcctcgttgt cgagggcctt gtagtcccaa
     2641 tgatcgccga ggacgtagtt gttgaacgcg aagggttgat cggaaacggc ggcgaagatg
     2701 gtgcccgtgc cgctctgatc gtcgacctgg aaggcgtcgc ggtcaccgcg ggcgcggatc
     2761 tcgaaggtct tgccaccctt gatgaagttg tcggcgccag gatcgagcgg gaacagcacg
     2821 cgcaaatgac cgcgggtatc ggcccgcagg acgatgacgt agccgtcgtc cgagagcttg
     2881 aacttcacgg tggcccggtc accgatctgg tagttgtcgt cgttgagcca aacgcggatg
     2941 gggggatcgt cggacgcttt gaaggcaggc gccggcttgg gcgccaccat cggcgcattc
     3001 agtacaaagg ctagagcggt gagcagcata gacccccctg gtgtcagata atacacctcg
     3061 gtccatagta gtgcatcctt tgtgccgggc cggacaggcg ctttattaca gggaaatcca
     3121 ccttttcaag ggtgccccgt gtctcatctg ctggacgctg taacggacgt ttcgaacgcg
     3181 tacgagctgg ctcccggttt tgtcacaggg gggcagccgc aggtggccca tttggcagcc
     3241 ctgaagaaag ccggctgtca ggtgctcctg gaccttcgcg aggccatgga ggagcgcccg
     3301 taccgggtcc ccgacgccgt ggtcggtgcg ggcctcgagt acgtcaacgt cccggtgggc
     3361 catggggctc agccggatga gacgttcacg aaggttcggg agcaggtccg gaatctggtg
     3421 ggcaagaagc cgaccttttg ctgctgcaat tccggcaatc gggtgggagc ggcgttgctt
     3481 ccttacttga tgctcgaccg cggcatggac gaggatgaag ccacgatggt
//
LOCUS       DASUBP010000234        11247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_934808, whole genome shotgun sequence.
ACCESSION   DASUBP010000234 DASUBP010000000
VERSION     DASUBP010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 11247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11247
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934808"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..708
                     /locus_tag="VFU75_08795"
     CDS             <1..708
                     /locus_tag="VFU75_08795"
                     /inference="COORDINATES: protein motif:HMM:NF019592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HEU5466975.1"
                     /translation="PATCWNAAETALAASFYAPGDSTQFNAGAYFDFSTDGGDATNDL
                     SDPLNGTTFYALPDFLVDADTQVGGAKDLRALSKIVPAQDTQILGGIPINGKLKFAVY
                     FTGGAVDLNHSIPIIRDEELALLDAEARIGLNDLTGANTILNLVRAGSGNLPADTLTG
                     GSPKAAYIAELLYNRRYSLMWEQGTRWVDARRYGLLANIPTDIPNTNVPPGAVPPVMP
                     IPDNECNARGLATGCAP"
     gene            complement(710..2368)
                     /locus_tag="VFU75_08800"
     CDS             complement(710..2368)
                     /locus_tag="VFU75_08800"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HEU5466976.1"
                     /translation="MRKALAVLAVATLALLAAPPLSAQGLRVEISVNPGFRPAPVTGR
                     MYVMFSRDSSPSPVSGVRGESDCVPFFGVDVTGLAAGTPGIIDGATLGYPLKSMADLP
                     PGDYYVQGLLSVYSEFKRADGHTVWAHDDQWEGQQFNRSPGNLLSEIRRVHVDPAHPD
                     TIRLELTKAIGPIDVAPDTREVRRVKIQSKLLTAFWGRPIYLGAVVLLPNNYDKDATT
                     RYPTVYEQGHFSLGAPFGFNPGGKPESPEARAERVKRTAREPGYEFAQAWNSPGFPQM
                     IAVTFQHPTPYFDDSYAVNSVNNGPYGDAILTELIPYLESHFRMIKAPSARLLVGGST
                     GGWEAMALQVYHPDFFGGTWVLYPDPLDLRAYELVNVYKDSNAFVFPVSEWFQADVPA
                     EREETGQPHITVRQESQYEAVLGSHARSGEQFAIWEATWGPVGSDGYPVDLWDLSTGK
                     INHQVSDYMKAHDWDIRDYLERNWATLGPKLRGKLHLYVGDMDTFYLNLSVYYVDAFL
                     KTAKPPADAVVEYGRPLKPHGWQPFTNAELMRQMAAYMARRPKR"
     gene            complement(2433..3968)
                     /locus_tag="VFU75_08805"
     CDS             complement(2433..3968)
                     /locus_tag="VFU75_08805"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HEU5466977.1"
                     /translation="MKQPSAIRGQPSTGRVPIAGRHLVLALLLAAGACVGPGYHPSPV
                     VAPSSQVGAASYSDSAKAFYDSLARARAADTLPTSGAAPLAPRRISADSVANLAWLDI
                     LHDSTLNALVQTALRQNRDLATAKARIEEYRADVGLARAPLFPSLTANGSASKNQVAF
                     GPQVFPYNAVRATVDVAWELDFWGKTRRGMQAANADLAAQDAVEQETVLSLISSVAIG
                     YLELLELDQEAQVSAHTLQTSQATLDLARQRYARGIISELDVRQFEAQVAAPAVRLAQ
                     VEQARAQTEHALNVLLGQGPTVIPRSGALADAARAVTVPDSLPSTLLSRRPDVRQAER
                     AYRAANARVGQTFAQQLPVIMVTGSYGSQAANTSGLFKPQSDVYVAQIGISIPLFAGG
                     ARSSATAAAKARAEQARNQYEQTVLRALQDAGDALSGARAARDQVAAAETQARALRRA
                     YDLAQVRYQSGIANYLEVLDAERSLFAAELTLSQAELGQLTAAVQLYKALGGSWNGMT
                     VSK"
     gene            complement(3965..7120)
                     /locus_tag="VFU75_08810"
     CDS             complement(3965..7120)
                     /locus_tag="VFU75_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0042908 - xenobiotic transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HEU5466978.1"
                     /translation="MSAGATAGSGSTGNNHQIKYFFIRRPVLAAVISIAITLMGIFSI
                     GLLPVSRYPTITPPAVQVAAVYPGATAQDVSDAVAGPIEEQLSGLQGLLYYTSANASD
                     GTMGLSVYFDVNRSQDLAQVDVQNAVQVATPQLPDAVRQNGLVILKANSDILAVVGLT
                     SKDPRYDAAYLTNYLKLYVEDELKRVPGVGNAQTFGGLQFSMLLQLNPDKMAQLGVTV
                     SQVAAAVREQNATNPAGRIGREPAPVGTQLTIPVTTQGRLTTPAQFDSIIVRSKADGS
                     VIRLGDIGHARLGSQSNDFAARLNGGPTAFDLIYLRPGANAIAVRDAVTKRMDELQTG
                     FPPGVTYSIPFDTTPFVTASIDEVVKTLIEAMILVSFVVFLFLQSWRATLIPMLAVPV
                     SVIGTFLGLRLLGMSINVLTLFALVLAIGIVVDDAIVVIENVERIMASEGVSARVASD
                     RGIQQVAGALVAIVLSLVAVFVPVAFTGGVTGELFKQFAITIVISVVLSGIVALTLTP
                     ALCAWLLKETNEAHASGFFGKFNAWFKNLTGNYAGAVDKVLGRPKAWLGAFAVLILLL
                     GFLWTHVQTAFVPTEDKGYFAVAVQLPDAASLQRTEAVVKRIEGFLHGEPAVRNVVAL
                     VGFDILSRASQSNSAVIFVNVKPWEERGKHDAIDAIVGRINGQLFMMPDAQSFAFNLP
                     EIFGLGTTAGVEANLQARSGQTVQQFAAQVQAFVAAANQLPAALGVQTTFRANVPQIY
                     VDVDRAAAKARGVQLSELYGTLQANLSTLYINDFNIYGKTYRVQAEAQAAYRESPADI
                     GRLYVRGTNDSMIPISALARVEFRSAPTLISRFNGFTSAFITAAPKPGHSSGELMQQM
                     DALIASQFGAQGLGLAYSGQSFQERASSGQSGLVFALGLILVFLVLAAQYESWSLPFA
                     VLLGIPFGVLGAYLGIWLRGQPSDIYVQVGLLTVVGLAAKNAILIVEFANELRDQGHS
                     IISAAVEAARERFRPILMTSLAFIFGVSPLVVASGAGAISRHSIGTTVFAGMVIATSI
                     GIFFIPLFFRLIRGFADRGQQ"
     gene            complement(7117..8187)
                     /locus_tag="VFU75_08815"
     CDS             complement(7117..8187)
                     /locus_tag="VFU75_08815"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5466979.1"
                     /translation="MALSLFSGCKKAGGFSGFPPSPVTIVTAKRAAVPESYQFPGAVL
                     PYRRVEVRSRVDGIIIAREFEEGQIVQPGQVLYQLDTVRYAAAYHSAEARLATAKSTY
                     NRVEPLLEQHAVAEQDVDNARSGLAAAQAAYDQAKKDYDDTQIKAEIEGRVGRTNLEV
                     GARVTGPADLLTTIDRIDPVYVSFRPSSAQLLDWQRRASSRALTRAGSALEIQAILPD
                     GSVYPRTGRLDFVAPSLDAATGTQEFRATFQNPDHLLMPGQFIQVRLVGFTRDSAIAV
                     PQRAVQTGLGGQFVYVVGAGDTVMPRDVVPGQWSGDQWIITSGLNAGDRVVVDGVQKV
                     IPGHTVAPKPLADATTVPGKDK"
     gene            complement(8344..9051)
                     /locus_tag="VFU75_08820"
     CDS             complement(8344..9051)
                     /locus_tag="VFU75_08820"
                     /inference="COORDINATES: protein motif:HMM:NF013586.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEU5466980.1"
                     /translation="MKALFLLLAVQAPAAPKVFRITPVRPVAELRAEALKATPPAEAG
                     PFRASELVEIVTLDSSIKLDIRYAGTDNFLSTPLYEQARAFLQRPAAEALVRVSHALR
                     AQGYGLLIHDAYRPWYITKIFWDATPKDKHDFVANPADGSRHNRGCAVDLSLYRLSDG
                     QAVDMPSVYDEMSERAYPTYTGGTDAQRHMRDFLRTAMEKEGFTVYPFEWWHFDYKDW
                     KQYGIQNIRFQEISAPK"
     gene            complement(9051..9320)
                     /locus_tag="VFU75_08825"
     CDS             complement(9051..9320)
                     /locus_tag="VFU75_08825"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HEU5466981.1"
                     /translation="MCRNIRTLYHFEPPATTDEVRAASLQFVRKVSGFTKPSTANQES
                     FDRAVDEVSQIVTRLLDSLVTTAPPKNREVEAAKAKARALKRFGT"
     gene            9378..10493
                     /locus_tag="VFU75_08830"
     CDS             9378..10493
                     /locus_tag="VFU75_08830"
                     /inference="COORDINATES: protein motif:HMM:NF016101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEU5466982.1"
                     /translation="MTRLLGLLPVFGAICVVACSAGNAAPPATQQPPPPSAPPSIDHV
                     FVIVLENRNFDVTFGPGSKGPYLADTLAKQGALLTQYYGIGHASLDNYIAMISGIAPN
                     PSTQGDCPVFTEFAEKGTSPDGQPIGLGCVYPAHVLTIGNQLAAAGKTWRAYLEDMGN
                     NPAREAPLCAHPAIGAVDPTERADNTKHDRYATKHNPWVYFHAVIDSASCSKNVVPLS
                     NLLTDLGSSTPNFSFIAPDLCHDGHDPDCGDEPGGLEGIDQFLSRWVPLITHSAAFQH
                     GLLVITFDESGNDDTACCAEPSGPNTTVPGGGGPGGGRTGAVLLSPYIAPGTISAVPY
                     NHYSFLRSVEDIFHLPYLGYAGAKGLVPFGSDVYTKK"
     gene            10493..10747
                     /locus_tag="VFU75_08835"
     CDS             10493..10747
                     /locus_tag="VFU75_08835"
                     /inference="COORDINATES: protein motif:HMM:NF025614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPXCG motif-containing cysteine-rich protein"
                     /protein_id="HEU5466983.1"
                     /translation="MVDFTDGPDDALTEEDFPLGDGTADGAALVTCPYCGEEVEITLD
                     PGGEEEQEYVEDCEVCCRPWNVTVHYGPAGVAEVRVERLE"
     gene            complement(10792..>11247)
                     /locus_tag="VFU75_08840"
     CDS             complement(10792..>11247)
                     /locus_tag="VFU75_08840"
                     /inference="COORDINATES: protein motif:HMM:NF014663.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466984.1"
                     /translation="IKTGDRIPVGRVPTAPPRKPEGEPPYASGVLRVVEGPQRALFSD
                     AEWNRFLGTSWTVSRVSDRMGYRLEGDVPVRAPAADLPSEAACVGAIQVPSDGMPIVL
                     MADGPTVGGYPKIAVVITADVPILAQKLPAQPVQFQETSVGEAQDALRR"
BASE COUNT         1895 a         3630 c         3851 g         1871 t
ORIGIN      
        1 cccgcaacct gctggaacgc ggccgagacc gccctcgcgg cttcgttcta tgctccggga
       61 gactccacgc agttcaacgc gggcgcgtac ttcgactttt ccacggacgg gggcgacgcc
      121 accaacgatc tctccgaccc gctgaacggc accacgttct acgcccttcc ggatttcctg
      181 gtcgatgccg acacccaggt gggcggggcc aaggatctgc gggcgctctc caagatcgtc
      241 ccggctcagg acacgcagat tctgggcggg atccccatca atggaaagct caagtttgcc
      301 gtgtacttca cgggtggggc ggtggacctg aatcactcca tcccgatcat ccgggatgaa
      361 gagctggccc tgctcgacgc ggaggcgagg atcggcctga acgacctcac cggcgcgaat
      421 acgatcctga acctggtgcg cgcggggtcg gggaacttgc ccgcggacac gctgacgggc
      481 ggcagtccga aggccgccta catcgcggag ctcttgtaca accgccgcta ctccctgatg
      541 tgggagcagg gcacgcggtg ggtggatgcc cggaggtacg ggctcctggc caacattccg
      601 accgacatcc ccaacaccaa tgtgcccccc ggtgcggtgc cgccggtgat gcccatcccg
      661 gacaacgaat gcaacgcccg gggtctcgcg acggggtgcg cgccctaggt cagcgcttgg
      721 gccggcgggc catgtaggcc gccatctggc gcatcaactc cgcgttggta aacggctgcc
      781 aaccatgcgg cttgaggggc cgcccgtatt cgacgacagc gtcggcgggc ggcttcgccg
      841 tcttcagaaa ggcatcgacg taatagacgg acaggttgag gtagaacgtg tccatgtctc
      901 cgacgtacag gtgcagcttg ccacgcagct tggggcccag agtggcccag ttgcgctcga
      961 ggtagtcgcg gatgtcccag tcgtgcgcct tcatgtagtc ggaaacctgg tgattgatct
     1021 tgcccgtcga gaggtcccac aggtccacgg gatatccgtc ggatcccacc ggcccccacg
     1081 tcgcttccca gatggcgaac tgctcgccgg agcgggcatg gctgcccagc accgcctcgt
     1141 actggctctc ctgccgcacg gtgatgtgcg gctgacccgt ttcctcacgc tcggccggca
     1201 cgtccgcctg gaaccactcc gagaccggaa agacgaacgc gttcgagtcc ttgtagacgt
     1261 tcaccagctc gtacgcgcgc aagtccagcg ggtccggata gagcacccag gtgccgccga
     1321 agaagtccgg gtgatagacc tgcagcgcca tcgcctccca gcctcccgtc gagccaccga
     1381 ccaggagccg cgccgacggt gccttgatca tccggaaatg cgattcgaga tacggaatca
     1441 gctcggtgag gatcgcgtca ccgtagggtc cgttgttcac ggagttcacc gcgtacgaat
     1501 cgtcgaaata cggtgtcgga tgctggaacg tcacggcgat catctgcggg aaccctggcg
     1561 agttccaggc ctgcgcgaac tcgtaccccg gctcccgggc cgtccgcttc actcgctcgg
     1621 cgcgtgcctc cggggactcc ggtttgccgc cggggttgaa tccgaacggc gcgcccaggc
     1681 tgaagtggcc ctgctcgtag acggtggggt accgggtggt cgcatccttg tcgtagttgt
     1741 tcggcaagag cactacggct ccgagataga tcgggcgccc ccagaacgcg gtgaggagct
     1801 tgctctgaat cttgacgcgc cgcacttcgc gggtgtcggg cgccacgtcg atgggtccga
     1861 tcgccttcgt gagctcgaga cggatggtgt cggggtgcgc cgggtctacg tgcacgcgcc
     1921 ggatctcgct caacaaattc cccggagagc gattgaattg ctggccctcc cactggtcgt
     1981 catgcgccca gacggtgtgg ccgtcggcgc gcttgaactc cgagtacacg ctgaggaggc
     2041 cctgcacgta gtagtcgccc ggcggaagat cggccatgga cttgagcgga tagccgaggg
     2101 tcgcgccgtc gatgatcccg ggcgtgccgg ccgcgaggcc ggtgacgtcc acgccgaaga
     2161 agggaacgca gtcgctctcg ccccgcactc cggacaccgg gctcggactc gagtcgcggc
     2221 tgaacatcac gtacatccgg cccgtcacgg gcgccgggcg gaatccggga ttgacggaga
     2281 tttcgacccg cagtccctgg gcggatagcg ggggggcggc gagcagggcg agtgttgcga
     2341 cggccaggac ggccagggcc ttacgcacgt agcggctcct ctggaggagc cgctacagtg
     2401 cacgcgttag cgagccggtg caaccccggg gactatttcg aaacggtcat cccgttccag
     2461 ctcccgccca gggccttgta cagctgtacc gccgccgtca gctgccccag ctcggcctgt
     2521 gagagcgtca gctccgccgc gaagaggctc cgctccgcgt ccagtacctc gaggtagttc
     2581 gcgatgcctg attggtaacg tacctgggca aggtcgtagg cccgccgcaa cgcgcgcgcc
     2641 tgcgtttccg ccgcggcgac ctggtcccgc gccgcccgcg cacctgacag tgcgtctccc
     2701 gcgtcctgca gggcccgcag caccgtctgc tcatactgat tccgggcctg ctcggcacgg
     2761 gccttcgccg ccgcggtcgc ggacgagcgc gcgccacccg cgaacagcgg gatggaaatg
     2821 ccgatctgcg ccacgtagac gtcggattgc ggcttgaaca acccgctcgt gttggccgcc
     2881 tgtgacccgt acgaccccgt gaccatgatg accgggagct gttgcgcgaa ggtttgtccc
     2941 acccgcgcat tcgccgcccg gtaagcccgc tccgcctgcc ggacgtcggg gcggcgcgac
     3001 aggagggtgg agggcaggga gtcggggacc gtcaccgccc gggccgcatc ggcgagtgcg
     3061 ccggaacgcg ggatgacggt cggaccctga ccgagcagta cgttcagcgc gtgctcggtc
     3121 tgggcgcggg cctgctccac ctgggcgaga cggacggcag gtgccgccac ctgcgcttcg
     3181 aattgccgca cgtcgagctc ggagatgatg ccccgggcgt accgctgacg cgccaggtcc
     3241 agggtggcct ggctggtctg cagcgtgtgc gccgacacct gggcttcctg gtcgagctcg
     3301 agcagttcca gatatccgat ggccacgctg ctgatcaggg aaagcacggt ctcctgctcc
     3361 acggcgtctt gcgccgcgag gtccgcgttg gcggcctgca tcccccgccg cgtcttgccc
     3421 cagaaatcga gctcccaggc gacgtctacg gtcgcgcgga ccgcgttgta cggaaacacc
     3481 tgcggaccga acgcaacctg gttcttggag gcgctcccgt tcgcggtcag ggacgggaag
     3541 aggggggcac gggccagtcc gacgtctgcg cggtactcct cgatccgtgc cttggcggtg
     3601 gccaggtccc ggttctgccg caacgccgtc tggacgaggg cgttcaacgt ggagtcgtgc
     3661 aggatgtcga gccaggccag gttggcgacc gaatcggccg agatccgccg cggcgccagg
     3721 ggtgcggcac ccgaagtggg cagcgtgtcg gccgcgcgcg cgcgggcgag cgagtcgtag
     3781 aacgccttgg ccgaatccga atagctggcg gcgccgacct gagacgacgg agcgaccacc
     3841 ggggaggggt gatagccggg tcccacgcag gcgcccgcgg cgaggaggag agccagcacg
     3901 agatggcggc cggcgatcgg cacgcgcccg gttgacggct gacctctgat ggctgatggc
     3961 tgcttcattg ctggccccga tccgcgaatc cccggatgag ccggaagaag agcggaatga
     4021 agaagatgcc gatggacgtg gcgatcacca tgccggcgaa caccgtcgtg ccgatcgagt
     4081 gacggctgat ggcgccggcg ccgctcgcga cgaccagagg cgagacgccg aagatgaacg
     4141 ccagggaggt catgagaatg gggcggaagc gctcgcgcgc cgcctcgacg gccgccgaaa
     4201 tgattgaatg gccctggtcc cgcagctcat tggcgaattc gacgatcaga atcgcgttct
     4261 tcgccgcgag tcccaccacc gtgagcaggc cgacctggac gtagatgtcg ctcggctgcc
     4321 ctcgcagcca gatgccgagg tatgcgccaa ggacgccgaa cgggattccc aggaggaccg
     4381 cgaagggcaa ggaccagctt tcgtactggg ccgcgagcac caggaagacg aggatgagac
     4441 ccagcgcgaa gacgagcccg gactggccgc tcgaggcacg ctcctggaag gactgtcccg
     4501 agtacgcgag cccgagcccc tgtgccccga actggctcgc gatcagggcg tccatctgct
     4561 gcatcagctc acccgagctg tgccccggct tcggggccgc ggtgatgaac gccgaggtga
     4621 agccgttgaa ccgcgaaatc agcgtgggag cgcttcggaa ctccacccgt gccaaggccg
     4681 aaatggggat catggagtcg ttggtgccgc ggacgtacag ccgcccgata tccgccgggg
     4741 attcccggta cgcggcctgg gcttcggcct gcacccggta cgtcttgccg taaatgttga
     4801 agtcgttgat gtagagcgtc gacaagttcg cctggagcgt gccgtacagc tcggagagct
     4861 gcacgccccg tgccttcgcg gccgcgcggt ccacgtccac gtagatctgc gggacgttgg
     4921 cccggaaggt ggtctgcacg ccgagggcgg cgggcagctg attcgccgcg gccacgaacg
     4981 cctggacctg ggcggcgaac tgctgcacgg tctggccgga gcgggcctgg aggttggcct
     5041 ccacgccggc ggtggtgccg aggccgaaga tctcggggag gttgaacgca aaggactggg
     5101 cgtccggcat catgaagagc tgcccgttga tgcgtcccac gatcgcatcg atcgcgtcgt
     5161 gctttccgcg ttcttcccag ggcttcacgt tgacgaagat caccgcgctg ttgctctggc
     5221 tggcgcggct caggatgtcg aacccgacca gcgcgacgac gttgcggacc gccggctccc
     5281 cgtgcaggaa gccttcgatg cgcttgacca cggcctccgt gcgctggagc gacgcggcgt
     5341 caggaagctg gacggccacc gcgaagtacc ctttgtcttc cgtcggcacg aatgcggtct
     5401 ggacgtgcgt ccacaggaag cccagcagca ggatgaggac ggcgaaggcg ccgagccacg
     5461 ccttcgggcg gccgagcacc ttgtcgacgg cgccggcgta attccccgtc aggttcttga
     5521 accaggcgtt gaacttcccg aagaatccgc tcgcgtgcgc ctcgttcgtc tccttcaaca
     5581 gccaggcgca cagggcgggc gtgagcgtca gcgccacgat gcccgagagc acgacggaga
     5641 tgacgatggt gatggcaaac tgcttgaaca gctcgccggt gacgccgccg gtgaaggcca
     5701 ccggcacgaa gaccgcgacg agggacagga cgatggcgac cagggcgccg gcgacctgtt
     5761 ggatgccccg gtccgacgcg acccgggccg agaccccttc cgaggccatg atgcgctcga
     5821 cgttttcgat gacgacgatc gcgtcgtcca ccacgatgcc gatggcgagg accagggcaa
     5881 agagcgtcag cacgttgatg gacattccca gcagccggag ccccaggaac gtcccgatca
     5941 ccgagaccgg taccgccagc atcggaatca gagtggcgcg ccagctctga aggaagagga
     6001 acacgacgaa ggagacgaga atcatcgcct cgatcagcgt cttgaccacc tcgtcgatcg
     6061 acgccgtgac gaagggggtc gtgtcgaacg ggatggagta ggtgaccccc ggggggaacc
     6121 cggtctgcag ctcgtccatc cgtttggtca cggcgtcccg gaccgcgatc gcgttggcgc
     6181 cggggcgcag atagatgagg tcgaacgctg tgggtccgcc gttcagccgg gccgcgaagt
     6241 cgttgctctg cgagcccagc cgggcgtggc cgatgtcgcc gagccggatg accgatccat
     6301 cggccttgga ccggacgatg atgctgtcga attgagccgg ggtggtgagg cgtccctggg
     6361 tggtgaccgg gatggtgagc tgggttccca cgggggccgg ctcacgtccg atgcgtccag
     6421 cagggttggt ggcgttctgc tcgcgcaccg ccgcggcgac ctggctcacg gtgacgccca
     6481 gctgggccat cttgtccgga ttcaactgca gcagcatcga gaattgcagc ccgccgaacg
     6541 tctgcgcgtt gccaactccg ggcacgcgtt tcagctcgtc ttccacgtac agcttcaggt
     6601 agttggtcag gtaggcggcg tcgtagcgcg ggtccttcga cgtcaatccc accacggcga
     6661 ggatgtcgct gttcgccttc agaatgacca ggccattctg gcggacggca tcgggcagct
     6721 ggggcgtcgc cacctgcacg gcgttctgga cgtcgacctg cgccaggtcc tgactgcggt
     6781 tcacgtcgaa gtagaccgag agccccatcg tgccgtcgga ggcgttggcg gacgtgtagt
     6841 agagcaggcc ctggagtccg gacagctgct cttcgatggg tccggccacg gcgtcggaaa
     6901 cgtcctgcgc cgtcgcgccc gggtacacgg ccgcgacctg cacggccggg ggggtgatgg
     6961 tcggataccg ggaaacgggc aggagtccga tcgagaagat ccccatcagg gtgatcgcga
     7021 tcgagatcac cgccgccagc accggccggc ggatgaagaa gtacttgatc tggtggttgt
     7081 tgccggtgct cccgcttccg gcggtcgctc cggcgctcac ttgtccttcc cggggacggt
     7141 cgtcgcatcg gcgaggggct tgggagcgac ggtgtggccc ggaatcactt tctgcactcc
     7201 gtccacgacc acgcggtcgc cagcgttcag gccgctggtg atgatccatt ggtcgccact
     7261 ccattggccc ggcacgacgt cgcgcggcat gacggtgtct ccggcgccca ccacgtacac
     7321 gaactggccg cccaggccgg tttgcacggc gcgctgggga acggcgatgg ccgagtcgcg
     7381 ggtgaatccc acgagacgga cctgaatgaa ctggcccggc atgagcaggt ggtccggatt
     7441 ctggaaggtg gcgcggaact cctgcgtgcc cgtcgcggcg tcgagcgagg gggcaacgaa
     7501 gtcgagccgg ccggtgcgcg ggtagacgga gccgtcgggc agaatcgcct ggatttcgag
     7561 ggcactgccg gcacgcgtca gcgcgcgtga ggacgcgcgg cgctgccaat cgagcagctg
     7621 cgccgaggac ggacggaacg agacgtagac gggatcgatg cgatcgatgg tggtgagcag
     7681 atcggcgggg ccggtgacgc gcgcgccgac ttcgagattg gtccgcccga cgcgtccctc
     7741 gatctccgct ttgatctggg tgtcgtcgta gtccttcttc gcctggtcgt aggccgcctg
     7801 cgccgccgcc agccccgagc gcgcgttgtc gacgtcctgc tcggcgaccg catgctgctc
     7861 gagcagcggc tccacgcgat tgtaggtcga cttggcggtc gcgagccgcg cttccgcgct
     7921 gtgataggcg gccgcgtacc gcaccgtgtc gagctggtag agaacctggc ccggttgcac
     7981 gatctgaccc tcttcgaact cgcgggcgat gatgatgccg tcgacgcgcg agcggacttc
     8041 cacccggcgg tacggcagca ccgcaccggg gaactgatac gactcgggca cggctgcgcg
     8101 tttggccgtc acgatggtga ccggggaggg cgggaagccg ctgaagccac ctgccttctt
     8161 gcagccggag aacagcgaca gtgccacgag ggcgcttaga gcagtcgtct tcatcatcat
     8221 ttctggtgtc ggggagaggt gtctatgaag aacgcgtaaa ggggcggaaa ggttttcgaa
     8281 ttctgggttc ttgagggtac ccaaagaaaa accgaagcgc aagggacccg gggtgggcgg
     8341 ggtctatttc ggggcggaaa tctcctggaa gcggatgttc tggatcccat actgtttcca
     8401 atccttgtag tcgaagtgcc accactcgaa ggggtacacc gtgaacccct ccttctccat
     8461 cgcggtccgc aggaagtccc gcatgtgccg ctgggcgtcg gtcccgccgg tataggtcgg
     8521 atacgcccgc tccgacatct catcgtagac gctcggcatg tccactgctt gaccgtccga
     8581 caggcgatag agtgacaggt ccaccgcaca gccccggttg tgccgggatc cgtcggccgg
     8641 attcgccacg aaatcgtgct tatcctttgg cgtcgcgtcc cagaagatct tggtgatgta
     8701 ccagggccga tacgcgtcgt ggatcagcag cccgtacccc tgcgcccgca aagcgtggct
     8761 tacacgcacc agggcttccg cggcgggccg ctgcagaaag gcccgcgcct gctcgtacag
     8821 cggggtgctc agaaagttgt cggttcccgc gtaacggata tccaatttga tagacgaatc
     8881 gagggtcacg atttcgacaa gctcggaggc tcggaacgga cctgcctccg ccggcggggt
     8941 ggccttgagc gcctcggcgc gcagctccgc gacgggcctg accggggtga tgcggaagac
     9001 ctttggcgcg gccggcgcct ggacggccag gagcaggaag agcgccttca tcaggtgccg
     9061 aacctcttga gggcccgggc cttggccttg gcggcctcga cttcccggtt cttcggtggc
     9121 gcggtggtga ccagagaatc gagcaacctg gtcacgatct gggacacctc atccacggcc
     9181 cggtcgaacg actcctggtt ggcagtagag ggcttggtga atcccgagac tttgcggacg
     9241 aactggaggg aagcggccct gacctcgtcc gtggtcgcgg gtggctcgaa atggtagagg
     9301 gttctgatgt tacggcacat aaggtgtctc cgggagatgc acggtaccaa ctgggaatct
     9361 gcggcgtaag ttcaagtatg accagactcc ttggattgtt acccgtcttt ggcgcgatct
     9421 gcgtggtcgc atgctcggct ggaaacgccg ctccgcccgc tacgcagcag cccccaccgc
     9481 cctcggcacc gccgtccatc gatcacgtct tcgtgatcgt gctcgagaac cggaacttcg
     9541 acgtgacctt cggccccgga agcaagggcc cgtacctggc ggacacgctc gcgaagcagg
     9601 gggcgctcct cacgcagtac tatggaatag ggcacgcgag tctcgacaac tacatcgcca
     9661 tgatttccgg catcgcgccc aatcccagca cgcagggtga ctgcccggtg ttcaccgagt
     9721 ttgcggagaa gggcacctct cccgacggcc agcccatcgg cctggggtgc gtctacccgg
     9781 cgcacgtgct cacgatcggc aatcagcttg cggccgcggg gaagacctgg cgggcgtatc
     9841 tggaagacat ggggaacaat ccggcgcgcg aggcgcccct gtgcgcccat ccggccatcg
     9901 gtgccgtcga tcccaccgag cgcgccgaca acaccaagca cgaccgctac gccaccaaac
     9961 acaacccgtg ggtctatttc cacgccgtga tcgattccgc gagctgctcg aagaacgtgg
    10021 tgcccctctc gaatctcctg accgacctgg ggtccagcac gcccaatttc tcgttcatcg
    10081 cgccggatct ctgccatgac ggccacgacc ctgattgcgg cgatgagccg ggtggcctgg
    10141 aaggaatcga ccagttcctt tcccgttggg tgcccctgat cacgcactcg gcggcgttcc
    10201 agcatggtct gctcgtgatc acgttcgacg aatcggggaa tgacgacacg gcgtgttgcg
    10261 ccgagccgtc ggggccgaac acgacggtgc cgggtggggg tgggccgggg ggcggtcgca
    10321 cgggggcggt cctgctttcg ccctacatcg cgccgggcac catctcggcg gtgccctaca
    10381 accactattc gttcttgcgg agcgtggagg acatcttcca cctcccgtac ctcggctacg
    10441 ccggcgccaa ggggctcgtg cccttcggct cggacgtgta cacgaagaag tagtggtgga
    10501 cttcaccgac ggtcccgacg acgcgctcac ggaagaggac ttcccgctgg gggacggcac
    10561 ggccgacggc gccgccctgg tgacgtgtcc ctattgcggc gaggaagtgg agatcacgct
    10621 ggatcccggt ggcgaagaag agcaggagta cgtagaggac tgcgaggtct gttgccggcc
    10681 ctggaatgtc acggtccact acgggcctgc gggagtggcg gaagtgcggg tggagcgact
    10741 cgagtgagac cgaccccggt cgtcgccctg atgttgattg cgaggcgcgc gtcagcggcg
    10801 tagcgcatcc tgcgcttccc cgaccgaggt ttcctgaaac tgcacgggct gggcagggag
    10861 tttctgggcg agaatcggga catccgcggt gatcaccact gcgatcttcg gatagcctcc
    10921 gaccgtcggt ccatccgcca tcagcacgat gggcatcccg tcggaaggga cctgaatcgc
    10981 gccgacgcag gcggcctccg aaggcaagtc ggcagccggg gccctcacgg gtacgtcacc
    11041 ttccagccga tatcccattc gatccgacac ccgcgacaca gtccacgatg taccgaggaa
    11101 ccgattccac tcggcgtctg agaacagcgc gcgctgaggg ccctccacta cccggaggac
    11161 cccggacgcg tacggtggct caccctcggg tttccttggc ggagccgtcg ggacccgacc
    11221 gacagggatg cggtcaccgg tcttgat
//
LOCUS       DASUBP010000235         2634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_852586, whole genome shotgun sequence.
ACCESSION   DASUBP010000235 DASUBP010000000
VERSION     DASUBP010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_852586"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..517
                     /locus_tag="VFU75_08845"
     CDS             <1..517
                     /locus_tag="VFU75_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466985.1"
                     /translation="TPAKKVALALTHLIRTQFPGDTLRVVLFHDTAEEIPIAQLAHAQ
                     VGPYHTNTAEGLKLARRILMGQRKDYRQIIMITDGKPSALTLPDGRIYVNSMGLDPQI
                     LRETYREVGLCRRSGILINTFMLARDRSLVEFVKQVSAICRGKAYFTNTMTLGQFILM
                     DFMRRKTRRVS"
     gene            523..2115
                     /locus_tag="VFU75_08850"
     CDS             523..2115
                     /locus_tag="VFU75_08850"
                     /inference="COORDINATES: protein motif:HMM:NF014427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEU5466986.1"
                     /translation="MGRPAVAAVTALLMGAACEAPFSIPAPVKYLRLAPDSVALETGD
                     TFALSVTATDSAGRPVSTPVDWASSAESLATVSASGVVITRLPGRVTITASAGGLIAG
                     VPISIAPRVTGVVIEQGDLVVSVGGSVAFTTFALDAQGDHLFGRPVAWAAQDPGIVDI
                     SSNGVALVRHSGATLVIASHGALSDSVAITAIDPHFTLLRAGPADHTCGLTDASVVLC
                     WGANDLGQLGVPALSQSATPIGSPHDPGLATVTAGATFTCGATGAGTVYCWGSSARGR
                     LGAGVNQLSTSKPVAVADSLFLDGLTSGWNHTCGLRAIEVICWGENPGAGGSGAITWN
                     PLIVTGAPEFASLSASVGFGCGLTVDSAAFCWGTNGAGQLGNGTIGETTVPIAVAGGL
                     KFAQVATGFMHACGLLATGAVECWGNGEQGQLGGGDTTSHFTPVPVSGGIRFVFLAAG
                     GARTCGLTADGSGYCWGDGVLVPTAIPGAVKFTSLTVGNAHACGLGTDARAYCWGRNN
                     RGQLGDGTLADRPTPTLVLGQP"
     gene            complement(2216..>2634)
                     /locus_tag="VFU75_08855"
     CDS             complement(2216..>2634)
                     /locus_tag="VFU75_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013104.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ammonia channel protein"
                     /protein_id="HEU5466987.1"
                     /translation="LCVGISYTAMQVRSKSMLDDSLDVFSCHGLAGIGGALLTGVFAS
                     KAANPAGGDGLLAGHPGQLGIQLVAVLAAAALAAAGTIVILKVVDVTMGARVGIHDEI
                     SGLDLSEHGEEAYVGGDVSGVSGSGIAIGQGVILSH"
BASE COUNT          414 a          924 c          858 g          438 t
ORIGIN      
        1 cacgccggcc aagaaggtgg ccctggcgct cacccatctc atccggacgc agttccccgg
       61 cgataccttg cgggtcgtcc tcttccacga cacggcggag gagattccca tcgcccagct
      121 ggcgcatgcc caggtcggtc cttaccacac caacacggcc gagggcctga agctggcgcg
      181 ccggattctg atggggcagc ggaaggacta ccggcagatc atcatgatca ccgacggcaa
      241 gccgtccgcg ctcacgcttc cggacgggcg gatctacgtc aactccatgg gactcgaccc
      301 gcagattctt cgcgagacct atcgtgaggt cggactctgc cgccggagcg gaattctcat
      361 caacaccttc atgctggcgc gggaccgcag cctggtggaa ttcgtgaagc aggtgagcgc
      421 catctgccgc ggaaaggcgt atttcaccaa cacgatgacc ctggggcagt tcatcctgat
      481 ggacttcatg cggcgcaaga cccggcgggt gtcctagccg tcgtgggccg ccccgccgtc
      541 gccgccgtca cggccctgct gatgggggcc gcatgcgaag cgccgttctc cattccggcc
      601 cccgtcaagt atctcaggct cgcacccgac agcgtggccc tcgaaaccgg cgatacgttc
      661 gccctgtcgg tcacggcgac ggactccgca ggccggccgg tgtccacgcc ggtcgactgg
      721 gccagctccg ccgagtccct cgcaaccgtg tccgcatcgg gtgtcgtcat cacccgtctt
      781 cccgggaggg tgacgatcac tgcctccgcc ggtggcctca tcgcaggggt ccccatcagc
      841 atcgccccga gggtcacggg cgttgtgatc gagcagggag acctcgtcgt ctccgtcggt
      901 gggtccgtcg cattcaccac cttcgcgctg gatgcccagg gcgatcactt gttcggccgg
      961 ccggtggcgt gggcagcgca ggacccaggc atcgtcgaca tctcgagcaa tggcgtcgcc
     1021 ctggtccggc actcgggggc gacgctcgtg atcgcgagcc acggggcgct gtccgactcg
     1081 gtggccatca ccgcgattga cccgcatttc acgctgctgc gagccggtcc ggccgaccac
     1141 acctgcggac tgacggacgc gtccgtggtg ctctgctggg gcgcgaacga tctgggccag
     1201 ctcggtgtcc ccgccctgtc tcagagtgcc acgcccatcg gctccccgca tgatccggga
     1261 ctcgccacgg tgactgccgg agcgacgttc acctgtggcg ccacgggcgc cgggacggtc
     1321 tactgctggg gttccagtgc gcgtgggaga ctgggcgccg gtgtcaacca gctgtccacg
     1381 tcgaaacccg tcgcggtcgc ggattcacta ttcctggacg gactcacgtc gggatggaat
     1441 cacacctgcg gtcttcgcgc catcgaggtg atttgttggg gtgagaaccc gggagcgggt
     1501 ggaagtggag cgattacctg gaatccgctg atcgtcaccg gcgcacccga attcgcgtcc
     1561 ctctcggcat ccgtcggatt cgggtgcgga ctcaccgtcg acagtgccgc gttctgctgg
     1621 ggtaccaacg gcgcgggcca actcggcaac gggaccatcg gggaaaccac cgtccccatc
     1681 gccgtcgccg ggggactgaa gttcgcccag gtcgcgacgg gattcatgca cgcgtgcggc
     1741 ctgctcgcga ccggcgccgt cgagtgctgg ggcaatgggg agcagggaca gctcggcggc
     1801 ggcgacacca ccagccactt cacaccggtc ccggtatcgg gcggcatccg cttcgtgttc
     1861 ctcgcggcgg gaggcgctcg tacctgcggc ctgacggccg acggctccgg gtattgctgg
     1921 ggtgacggcg tccttgtgcc aaccgccatc ccgggcgcgg tgaagttcac gtcattgacg
     1981 gtcggcaacg cgcacgcgtg tggcctcggc accgatgccc gcgcctattg ctggggccgg
     2041 aacaaccggg gtcagctggg ggatggaacg cttgccgacc ggcccacgcc gacactcgtc
     2101 ctgggtcaac cctgatccac ttcagaaacg cggaagtcgg aaggcggaat ggggaaccgg
     2161 ccggtcggtt ccgcgctccg cgttctcact tccgcgtttg gccaggttga agaggtcagt
     2221 gcgagaggat cacgccctgc ccgatcgcga ttcctgaacc cgacacaccg gacacatccc
     2281 cacccacata cgcttcctct ccgtgctcgc tcaggtcgag cccggagatc tcgtcgtgga
     2341 tccccacccg cgcgcccatg gtcacgtcga ccaccttgag gatcacgatc gtgccggccg
     2401 cggcgagcgc cgccgccgcc agcaccgcga ccaactgaat cccgagctgc ccggggtggc
     2461 cggcgagtag tccatctccc cccgccgggt tcgcggcctt cgaggcgaat acgccggtca
     2521 gaagggctcc cccgatcccc gccagcccgt ggcacgagaa gacgtcgagc gagtcgtcca
     2581 acatcgactt ggaccgcacc tgcatggccg tatagctgat gccgacgcac agca
//
LOCUS       DASUBP010000236         6557 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_920907, whole genome shotgun sequence.
ACCESSION   DASUBP010000236 DASUBP010000000
VERSION     DASUBP010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6557)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6557
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_920907"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..771)
                     /locus_tag="VFU75_08860"
     CDS             complement(130..771)
                     /locus_tag="VFU75_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466988.1"
                     /translation="MKRTWILVALTVGSAAPASAQFIGMPVWNSPSGGTGLTISGDYG
                     KPNDNNFGGGSAFGGRATLGIGMLSVTAGVASFSPSPAGASKITSLGGDVAFQVIGGP
                     LVPVKVNIQGGAAHTSADSGITVVTGALGIGVTLPMPGISIEPYISPGIRYTKVNTLF
                     GSSKANFGFAIGANVGFGMLGVHIAYDHSKLLGQNVSTFGVGAHLALKVPLGM"
     gene            complement(983..1507)
                     /locus_tag="VFU75_08865"
     CDS             complement(983..1507)
                     /locus_tag="VFU75_08865"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU5466989.1"
                     /translation="MKRTLRWLVMVALIAGAGRLSAQRIMVGGGLLMPMGDYKTVDKM
                     GFIGQAGVGFPAGPIGIRIEADYGQTSHKNGADGNSKIIGGMGAAVYHFKTPAGVTPY
                     VLGGVGMYNVKVTVPSISFDQSETKFAFGVGAGLEMKMTSMSLYLEAKYMNIATSGGA
                     TTFIPITVGLRFGM"
     gene            complement(1638..2198)
                     /locus_tag="VFU75_08870"
     CDS             complement(1638..2198)
                     /locus_tag="VFU75_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5466990.1"
                     /translation="MRRTLGSLLVVTGMMLAPALAAAQHNMGAPAHEIGVDLGVSYLK
                     PSGLHSIFTIGTPVDARLGFVMHSGWTLEPRVFILIQSAAGTAHDVALDVNGLFPVGT
                     GSNYRKGMYLTGGGGLEFLGGNLSGLPSVSGTVFELNGGLGTRVPYESGAIRLEANVK
                     YRFQDTSIGVPNTFEIGGRIGLSLWH"
     gene            complement(2262..2510)
                     /gene="atpC"
                     /locus_tag="VFU75_08875"
     CDS             complement(2262..2510)
                     /gene="atpC"
                     /locus_tag="VFU75_08875"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HEU5466991.1"
                     /translation="MRVVVISPEQSVFDGEADQVVAPAFDGEVGILPRHAPFMTLLGK
                     GVLSVRRGNTSSRFDVSGGFLQVLNDVVRVVAGSAQSA"
     gene            complement(2510..3958)
                     /gene="atpD"
                     /locus_tag="VFU75_08880"
     CDS             complement(2510..3958)
                     /gene="atpD"
                     /locus_tag="VFU75_08880"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEU5466992.1"
                     /translation="MATKTKSNAKKPTGESAQNTGRVVQVIGPVLDVEFEPEHLPELY
                     NAVVVEHSGGRLVAEVQQHIGRNQARAVAMSSTDGVVRGMPVLDTGSPITVPVGKAAL
                     GRILNVLGEPVDEGAAIPASAERWPIHRETPKFVDLEPKTEIFETGIKVVDLIAPFVK
                     GGKIGLFGGAGVGKTVIIQELIHNVALGHGGRSVFCGVGERTREGNDLYLEMKESGVI
                     DSCSLIYGQMNEPPGARLRVGLTGLTVAEYFRDVEGQDVLVFIDNIFRFTQAGSEVSA
                     LLGRMPSAVGYQPTLATEMGALQERITSTKKGSITSVQAIYVPADDLTDPAPATAFSH
                     LDATVVLSRQIVELGIYPAVDPLASSSRILDAQYLGERHYKVATEVQRILQRYKDLQD
                     IIAILGMDELSEEDKTLVARARRVQRFLSQPFFVASQFTGLEGKYVKLEETVSSFERV
                     VSGEFDSLPEQAFYMQGSIDDVIAQAKKMASA"
     gene            complement(4060..4935)
                     /gene="atpG"
                     /locus_tag="VFU75_08885"
     CDS             complement(4060..4935)
                     /gene="atpG"
                     /locus_tag="VFU75_08885"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HEU5466993.1"
                     /translation="MAQARELRRRIKSVQSTRQITRTMEMVATSKLKRAQDRVVAARP
                     YANALAEVIGDLYAPDLAERFPLLRQPGEKGVKRVALIGVTANRGLCGAFNTNLIREM
                     RRRIADLEGQGAQVDMHLVGRKGAAFFRFTKRAVASQRTDIGERPTAEHAQEIITPVL
                     EQFAKGELDAVEIVFAKFASAISTPATTVRILPIAPPEAKAGGVKADYLLKPSAEAIL
                     EELLPLYVRNQVYRALVETTAGFYGSQRTAMKNATDNAGEMLDLLKRTYNRARQGQIT
                     QELAEIVGGAEALNG"
     gene            complement(4935..6476)
                     /gene="atpA"
                     /locus_tag="VFU75_08890"
     CDS             complement(4935..6476)
                     /gene="atpA"
                     /locus_tag="VFU75_08890"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEU5466994.1"
                     /translation="MATDSILRPGELKDILLKEIQAADLSDIDVREVGTVLEVKDGIA
                     RIYGLTSAMAGEMLEVTASGSGEKITGLALNLEEDNIGAVILGDYLQLREGDEVRRTA
                     RVLEVPVGPALVGRVVNPLGQPVDGLGPIKTTTTRKVEIVAPGIIKRQPVKEPLQTGV
                     KAIDAMIPIGRGQRELIIGDRGTGKTAIAVDTIINQKGQGVICVYVAIGQKNSTVASV
                     VERLKEHGAMAYTIVVVASASEPAPLQYIAPYSGCTMAEYFMYNEKKATLCVYDDLSK
                     QAAAYRQLSLVLRRPPGREAYPGDVFYLHSRLLERAAKLSDEMGGGSLTALPIIETQA
                     GDVSAYIPTNVISITDGQIFLETDLFYSNVRPAVNAGISVSRVGGNAQIKAMKQVAGR
                     LRLDLAQYRELEAFAQFGSDLDAATQRQLARGVRTVEVLKQPQYEPMPVEQQIMIIYA
                     VTNGLLDDVDVKELRAWEKGFHEFMKAQHPEIGDEIRTKKTMSDDCQARLKKAIDEYK
                     AVGAR"
BASE COUNT         1148 a         2156 c         2076 g         1177 t
ORIGIN      
        1 ccaggagcag ggcgatcgcg gcaagggcgg tcaggggtcg caacgggtct cccgaatgtg
       61 ttttggggcg cgccctagac gcaggacgcg ccccaaagct accgacggcg gcggtgaatg
      121 tgaacgggct tacatcccca gcggcacttt cagcgccagg tgggcaccga caccgaacgt
      181 gctgacgttc tgcccgagaa gcttgctgtg atcatacgcg atgtggacgc cgagcatccc
      241 gaagccgacg ttggcaccga tggcgaagcc gaaattggcc ttgctgctgc cgaacagggt
      301 gttgaccttc gtataccgga ttcccggaga gatgtagggc tcgatgctga tgcccggcat
      361 ggggagggtc actccgatgc caagcgctcc ggtcacaacg gtgatcccgg agtccgcgga
      421 ggtatgcgcc gcgcctccct ggatgttgac cttcaccggt acgagagggc cgccgatcac
      481 ctggaacgcc acgtccccgc ccagagacgt gatttttgat gcaccggccg ggctggggct
      541 gaagctcgcg acgcccgcgg tcaccgacaa catgccgatg ccgagtgtcg cgcgacctcc
      601 gaaggcactt cccccaccga aattattgtc attcggtttg ccgtagtcgc cgctgatggt
      661 caagccggtt ccgccagacg ggctgttcca caccggcatg ccgatgaact gggcagacgc
      721 gggagccgcg ctgccgacag taagtgctac caggatccag gttcgcttca tgagtgccct
      781 ccgctcgggt gagtacgtct gtaacccgcc caacggtagg aagtcacttc cggcaagtca
      841 acgtgtcagc ccatttcggc gacagcggcc caatgacacg ccgttccgaa tacacgccac
      901 gcgggccatg ggttccccta tcgcaaaaag caaaccgccg cccggctggg aagccggacg
      961 gcggttggca aatgcgggtg cgctacatgc cgaagcgcag tccgaccgta atcggtatga
     1021 acgtcgtcgc gccgccactc gtcgcgatgt tcatgtactt ggcttcgagg taaagactca
     1081 tggacgtcat tttcatctcc aaccctgcgc cgacgccgaa tgcgaacttg gtctcggatt
     1141 ggtcaaaact gatgctcggt acggtgacct tcacgttata catgcctacg ccgcccagca
     1201 cgtatggcgt gacgccggca ggggtcttga agtgatagac cgcggcgccc atcccgccga
     1261 tgattttcga atttccgtcg gcgccgttct tgtggctggt ctgtccgtaa tcggcttcaa
     1321 tccggatgcc gatcggtccc gcagggaacc cgaccccggc ctgcccgatg aatcccatct
     1381 tgtcgacggt cttgtaatcg cccatcggca tgagcagacc accgcctacc atgatgcgct
     1441 gcgcactgag cctgccggcg ccggcgatca gcgccaccat cacgagccac cgcaatgtcc
     1501 gcttcatgga ttccccctcc aggagtggtg agaagagccc cgagacggcc gctactaaat
     1561 gggaaatccc ccctgcaaag tcaataaaaa gggtccggcg gagcacgccg gaccccggaa
     1621 acacgtccac tctgcggcta atgccaaagc gacaggccga tccggcctcc gatttcaaag
     1681 gtgttcggga cgccgatact cgtgtcctgg aaccgatact tcacattggc ctccaggcga
     1741 atggcgccgg actcatacgg cacccgggtt ccaagtccgc cgttgagctc gaagacggtc
     1801 ccactgacac tggggagtcc gctgagattg ccgcccaaga actcgagccc accgccaccc
     1861 gtcaggtaca tccccttgcg gtagttgctt ccggttccga cggggaagag tccgtttacg
     1921 tcgagagcga catcgtgagc ggtgccggcg gcggattgaa tcaggatgaa gacccgcggc
     1981 tcgagcgtcc atcccgagtg catgacgaag ccgagacggg catcgaccgg cgtcccgatc
     2041 gtgaagatcg agtgcaagcc agacggtttg aggtaagaaa caccaaggtc gacaccgatc
     2101 tcgtgggccg gcgcacccat attgtgctgc gctgcggcga gagccggagc cagcatcatc
     2161 cctgtgacca ccaggagact accgagtgtt ctacgcatac gttctccctc caaaggagtt
     2221 agggaacggc gtagaactgt tagcgaggca ggttcagcgg gtcaagcgga ttgggcgctt
     2281 ccggcgacga ctctgacaac gtcattgagt acctgcagaa atccacctga aacgtcgaag
     2341 cggctggacg tgttgccgcg gcgcaccgac aacacaccct tccccagcag cgtcatgaac
     2401 ggcgcgtgcc gggggaggat gccgacttcc ccgtcgaagg cgggcgcgac cacctggtcg
     2461 gcctcgccat cgaacacgga ctgctcggga gagatgacga cgacgcgcat caggccgagg
     2521 ccatcttctt cgcctgcgcg atcacgtcgt cgatcgagcc ctgcatgtaa aacgcctgct
     2581 cgggcagcga atcgaattcg ccggacacca cgcgctcgaa gctcgacacc gtctcctcga
     2641 gcttcacgta cttgccctcg agcccggtaa actgcgatgc gacgaagaac ggctgcgaca
     2701 ggaagcgctg gacccggcgc gcgcgcgcca ccagcgtctt gtcctcttcg gaaagctcgt
     2761 ccatcccgag aatcgcgatg atgtcctgca gatccttgta gcgctgcaga atccgctgca
     2821 cctcagtcgc caccttgtag tgccgctcgc ccaggtactg cgcgtcgaga atccgcgaag
     2881 acgacgccag ggggtcgacg gcggggtaga tgccgagctc cacgatctgc cgcgagagca
     2941 cgaccgttgc atcgaggtgc gagaacgccg tggccggtgc cggatcggtc aggtcgtcgg
     3001 cgggcacgta gatcgcctgc accgacgtaa tcgacccctt cttggtcgac gtgatccgct
     3061 cctgcaaggc acccatctcg gtcgccagcg tcggctgata tcccaccgcc gagggcatgc
     3121 ggccgaggag tgcggacacc tcggagccgg cctgcgtgaa ccggaagata ttgtcgatga
     3181 agacgagcac gtcctggccc tcgacgtcgc ggaaatactc ggcgacggtg aggcccgtca
     3241 aaccgacccg gagacgcgcg ccggggggct cgttcatctg gccatagatc agggagcagg
     3301 agtcgatgac gcccgattcc ttcatttcca gatagaggtc gttgccttcg cgcgtccgct
     3361 cgcccacgcc gcagaacacc gagcggccgc cgtggcccag ggccacgttg tggatgagct
     3421 cctgaatgat gacggtcttc cccacgccgg cgccgccgaa gagcccgatc ttgccgccct
     3481 tcacgaaggg ggcaatcaga tcgaccacct tgatgcccgt ctcgaagatc tcggtcttgg
     3541 gctcgagatc cacgaacttc ggcgtctccc ggtgaatggg ccagcgctcg gcagatgccg
     3601 ggatcgcggc cccctcatcg accggctcgc cgaggacgtt gaggatgcgc ccgagtgcgg
     3661 ccttccccac cgggaccgta atcgggctcc cggtatcgag cacgggcatc ccgcgcacca
     3721 cgccgtcggt ggagctcatc gcgaccgcgc gggcctggtt ccggccgatg tgctgctgca
     3781 cttcggccac tagccggccc cccgagtgct ccaccaccac ggcgttgtac agctccggga
     3841 ggtgctcggg ctcgaactcg acgtcgagga ccgggccgat cacctgcacc acgcggccgg
     3901 tgttctgcgc cgactcgccc gtcggcttct ttgcgttgct cttggtcttg gtcgccatgc
     3961 aactcctcag gggtcagggg tcaggggtca ggggtcagat cgggtcttcg aggatcggtc
     4021 cgtgcggcac tgatccctga cccctggccc ctggcccctc tatccgttca gcgcctcggc
     4081 gcctccgaca atctccgcca attcctgggt aatctgtccc tgccgcgccc ggttgtacgt
     4141 ccgcttgagg aggtcgagca tctcaccggc gttgtcggtc gcgttcttca tcgcggtccg
     4201 ctgggagccg tagaatccgg cggtcgtctc caccagcgcc cggtacacct gattgcgcac
     4261 atacaagggc agcagctctt cgagaatcgc ttcggccgag ggcttgagca ggtagtcggc
     4321 cttgacgccg ccggccttcg cttcgggggg cgctatcggc agaatccgca ccgtcgtcgc
     4381 gggcgtcgag atcgcgctgg cgaacttcgc gaagacgatc tccaccgcgt ccaactcccc
     4441 cttggcgaac tgctccagca ccggcgtgat gatttcctgc gcatgctccg ccgtcggccg
     4501 ctcgccgatg tcggtgcgct gcgatgccac cgcacgcttg gtgaaccgga agaaggccgc
     4561 gcccttccgg cccacgagat gcatgtccac ctgcgcgccc tgcccctcca ggtccgcgat
     4621 gcggcggcgc atctcccgga tcaggttggt gttgaaggcg ccgcagagcc cccgattggc
     4681 ggtaaccccg atcagggcga cccgcttcac acccttctcg ccgggctggc gcaggagggg
     4741 aaagcgctcg gccaggtcgg gcgcgtacag atcgccgatc acctcggcca gggcgttggc
     4801 gtacggacgg gccgcgacga cgcggtcctg ggcgcgcttc agcttggacg tggccaccat
     4861 ctccatcgtc cgcgtgatct gccgcgtgct ctggaccgac ttgatgcggc ggcgaagctc
     4921 ccgggcctga gccattagcg ggcgccgacc gccttgtact cgtcgatcgc cttcttcaga
     4981 cgggcctggc agtcgtcgga cattgtcttc ttggtgcgga tctcgtcacc gatttcgggg
     5041 tgctgggcct tcatgaactc gtggaagccc ttctcccagg cgcgcagctc cttcacgtcc
     5101 acgtcgtcga gcaatccgtt ggtgacggcg tagatgatca tgatctgctg ctccaccggc
     5161 atcggctcgt attgcggctg cttcaggact tcgacggtcc ggacgccccg cgccagctga
     5221 cgctgagtcg cggcgtcgag gtcggagccg aactgcgcga aggcttcgag ctcgcggtac
     5281 tgcgccagat cgagccggag acggccggcg acctgcttca tcgccttgat ctgcgcgttc
     5341 ccgcccacgc gcgacaccga gatgccggcg ttcaccgcgg gacggacgtt cgaatagaag
     5401 aggtcggtct cgaggaagat ctggccgtcg gtgatggaaa tgacgttcgt cgggatgtac
     5461 gccgagacgt cgccggcctg cgtttcgatg atcgggagcg cggtcagcga gccgccaccc
     5521 atctcatcag agagcttggc ggcgcgctcg agcagacggc tgtggagata gaagacgtcg
     5581 ccgggatacg cttcgcggcc ggggggccgg cggagcacca gggacagctg gcggtacgcc
     5641 gcggcctgct tggacaggtc gtcgtacacg cacaacgtcg ccttcttctc attgtacatg
     5701 aagtattcgg ccatcgtgca accggagtac ggcgcgatgt actgcagggg cgcgggctcc
     5761 gaggcggaag cgacgaccac gatggtgtac gccatcgcgc catgctcctt gagccgctcc
     5821 accaccgacg ccacggtcga attcttctgg ccgatggcga cgtagacgca gatgacgccc
     5881 tggcccttct gattgatgat ggtgtcgacc gcgatggcgg tcttgcccgt gccccggtcg
     5941 ccgatgatca gctcgcgctg accgcggccg atcgggatca tcgcgtcgat cgctttgacg
     6001 cccgtctgca ggggctcctt caccggctgc cgcttgatga tgccgggagc gacgatctcg
     6061 accttccggg tcgtcgtcgt cttgatcggg cccagtccgt cgaccggctg tcccagtgga
     6121 ttgactacgc gcccgacgag ggcgggaccg accggcacct cgagcacgcg ggcggtgcgc
     6181 cggacctcat cgccttcgcg gagctggagg tagtccccca gaatcacggc gccgatgttg
     6241 tcctcttcga ggttcagcgc caggccggtg atcttctcgc cggacccgga ggccgtcacc
     6301 tcgagcattt cgcccgccat cgcggaggtg agaccgtaaa tgcgagcgat tccgtccttc
     6361 acttccagca cggttccgac ttcgcggacg tcgatgtccg agaggtcggc cgcctgaatt
     6421 tccttgagaa ggatgtcttt cagctcacca ggtcgcagga tggaatcggt cgccataagc
     6481 cgtcgtgaga gagattgtga aaaatatcac aaggaagggg tgctcggcaa gcgggctacc
     6541 caagctgaag cttgggc
//
LOCUS       DASUBP010000237         2244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_117202, whole genome shotgun sequence.
ACCESSION   DASUBP010000237 DASUBP010000000
VERSION     DASUBP010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117202"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1073)
                     /locus_tag="VFU75_08895"
     CDS             complement(<1..1073)
                     /locus_tag="VFU75_08895"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5466995.1"
                     /translation="MDEQLIPIIGDAYIEGKFPKQLIPGMAELGLFGANLPEEYGCAG
                     LNNVAYGLIMQELERGDSGVRSFASVQGALVMYPIYAFGSDDQKKKWLPEMASGKVIG
                     CFGLTEPDYGSNPAGMITTAKETKDGWVLNGAKMWITNGSTAQVAVVWAKTGAQNDAN
                     SIRGFIVPTHTKGFSAKDQKGKLSLRASDTSELVLQDVHVAKDAILPKSGGLKSPLMC
                     LTQARYGISWGALGAAMACYDEAVSYSKNRVMFGKPIGGFQLQQARLAEMLTEITKAQ
                     LLVLQLGRLKDQGKMTPQQVSLAKRNNVDMATDIARETRRLLGANGILAEYQAMRHLA
                     NLESVYTYEGTHDVHTLVLGQEIT"
     gene            complement(1288..2151)
                     /locus_tag="VFU75_08900"
     CDS             complement(1288..2151)
                     /locus_tag="VFU75_08900"
                     /inference="COORDINATES: protein motif:HMM:NF024258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU5466996.1"
                     /translation="MTVQILHLSDLHFGGLADLRQIEALEAILPDLRPDAVVISGDLS
                     QRARHGEFQRARAFMNMAKETSPVHVIPGNHDVQWWWRPFIPFGTRAKYEKYRRYFGE
                     DVTPALHIPGAVIAGANTSHGIAWGSLTPRLRDIAVKGHLPKSEILRVKQIFDAADPA
                     DARVLVTHHNVLRGDISQRMGLARWRQAQRRILESGADVVLCGHDHQEGADVLGGRVV
                     VSTSGTLSTRSRGGRPSCFNFVTIEPTAVQVTFFRWEQDRGRWRASDTFAFARGGAHS
                     DARRDSVGVAQ"
BASE COUNT          378 a          726 c          732 g          408 t
ORIGIN      
        1 gtgatttcct ggcccaacac cagcgtatgc acgtcgtgcg tgccttcgta ggtgtagacc
       61 gattccagat tcgcgagatg ccgcatggcc tgatactccg cgagaatccc gttggcgccc
      121 agcaggcggc gggtttcgcg ggcgatgtcc gtggccatgt cgacgttgtt gcgcttcgcg
      181 agggaaactt gctggggcgt catcttgccc tggtccttca ggcgtcccag ctggagcacg
      241 aggagctgcg ctttcgtgat ttcggtgagc atctctgcca ggcgggcctg ctggagctgg
      301 aagccgccga tcggcttgcc gaacatgacc cggttcttcg agtagctcac cgcttcgtca
      361 taacacgcca tcgcggcacc cagcgcgccc cacgaaatgc cgtagcgcgc ctgggtcagg
      421 cacatcaagg gggacttgag cccgcccgac ttcggcagaa tggcgtcctt cgcgacgtgg
      481 acatcctgga gcactagctc gctggtgtcc gacgcgcgga gtgacagctt gcccttctga
      541 tccttcgcgg agaagccctt ggtgtgcgtg ggaacgatga agccgcggat cgagttggcg
      601 tcgttctggg cgcccgtctt ggcccagacc acggcgacct gggcggtcga gccgttcgtg
      661 atccacatct tggcaccgtt caggacccag ccgtccttcg tctccttggc ggtcgtgatc
      721 atgccggcgg ggttcgagcc gtagtcgggc tcggtgagtc cgaagcaccc gatcactttt
      781 ccgctcgcca tctccggcag ccacttcttt ttctggtcgt ccgatccaaa cgcgtagatg
      841 gggtacatca ccagcgcgcc ctgcaccgac gcgaacgacc gcacacccga atcgccgcgc
      901 tcgagctcct gcatgatgag cccgtacgcg acgttgttca gtccggcaca cccgtattcc
      961 tcgggcaggt tcgcgccgaa caaccccaac tcggccatgc ccgggatcaa ttgcttgggg
     1021 aattttccct cgatataggc gtcgccgatg atagggatga gctgctcatc cacccaggtc
     1081 cggacggtgt cgcggacggc gcgctcttct tcgttcagaa gggtgtcgat cgcgtagaaa
     1141 tcgacgccgt ggtatttcgg ggccagaaga gacctcagag gtcaggggtc agtagtcagt
     1201 ggccagtaaa gctaaccccc gaagcggcct caactaaccc cgggcgcgag cccggggttg
     1261 atcgaaaagt cgttgtcttg ttggggttta ctgcgctacc ccgaccgagt ccctgcgggc
     1321 atccgaatgc gccccgccgc gggcaaaggc gaaggtgtcc gaggcccgcc accggccccg
     1381 gtcctgttcc caccggaaga acgtcacctg cacggcggta ggctcgatgg tgacgaagtt
     1441 gaagcaggag gggcgtccgc cccgtgaacg cgtggagagg gtacccgagg tagacaccac
     1501 cacccggccc ccgagaacgt ccgcgccctc ctgatgatcg tgaccgcaca gcacgacgtc
     1561 ggcgcccgac tcgagaatcc ggcgctgggc ctgccgccac cgggcgagcc ccatccgctg
     1621 actgatgtct ccccggagca cgttgtgatg cgtcaccagc acccgggcat cggccggatc
     1681 ggctgcatcg aagatctgct tcacccggag gatttcggac ttggggaggt ggcccttcac
     1741 ggcgatgtcc cgcagccgag gggtgaggga gccccacgcg attccgtggg aggtgttggc
     1801 cccggcgatc acggcgcccg ggatgtggag cgccggcgtg acgtcttcac cgaagtacct
     1861 gcggtacttc tcgtacttgg cccgcgtgcc gaacgggatg aacggacgcc accaccactg
     1921 cacgtcgtgg ttgccgggga tcacgtgcac cggcgacgtc tccttggcca tgttcatgaa
     1981 cgcgcgggcg cgctggaact cgccgtgccg ggcgcgctgg gacagatcac cggagatgac
     2041 gaccgcgtcg ggacgcaggt cgggcaggat ggcctcgagc gcctcgatct ggcggaggtc
     2101 cgccaggccg ccgaaatgca gatccgacag atggagaatc tgaaccgtca cgaatccacc
     2161 tgtaccgtgt aggtcagagt cgcggtgccg ttggcgggca ccgagacccg gaagcgctgt
     2221 tccgtcgagg acaacttctc cgcg
//
LOCUS       DASUBP010000238        19425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_307454, whole genome shotgun sequence.
ACCESSION   DASUBP010000238 DASUBP010000000
VERSION     DASUBP010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 19425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19425
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_307454"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..979)
                     /locus_tag="VFU75_08905"
     CDS             complement(<1..979)
                     /locus_tag="VFU75_08905"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEU5466997.1"
                     /translation="MQDIVDITIIGAGPTGLFGAFYAGMRGASCRIIDNLDQVGGQLA
                     ALYPEKYIFDVAGFPKILAKDLVKGLAEQAAQFRAPVHLGQKVVGLKKEDDGRPLFII
                     TTDQDQFPSRTVIIAGGIGAFTPRKLPLKDADQWLGRGLHDRVLDPKVFQDKRVLIVG
                     GGDSAFDWTVNLHPIAKSILMIHRRDGFRAHQATVDQVQQMHASKQVELRTFWELKAI
                     QGGGQIESVVLYNNQTKAEETHAMDCVLPQLGFVSSLGAIAEWGLEIEKHEIKVSQTM
                     STNVPGIYAAGDMATYPGKLKLIATGVAEASIAVNHAVHFINPSAKFEPG"
     gene            1073..1612
                     /locus_tag="VFU75_08910"
     CDS             1073..1612
                     /locus_tag="VFU75_08910"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HEU5466998.1"
                     /translation="MKIYTKTGDAGDTGLFGGGRVPKDHPRVAAYGEVDSLNAAVGFA
                     ASLEPATWEGALLEGIQRDLFTTGAILATPDPTKPKTPPAPITAERITALEQAIDRLE
                     HGLPPLDRFILPGGGPKGAAFHVARTTCRRAERAVVTLARSETIPKEVLIYLNRLSDL
                     LFVLARSANQAAGHPELQW"
     gene            1606..2712
                     /gene="aroB"
                     /locus_tag="VFU75_08915"
     CDS             1606..2712
                     /gene="aroB"
                     /locus_tag="VFU75_08915"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEU5466999.1"
                     /translation="MVTVRVALSAESGGERSASYDVLVGRGLLKDLTTLLSRHAPASR
                     YVVITDDQIERTFARKAANDLSAPLLSFPAGEGRKTRETWADLTDRLLAHHVGRDACI
                     VAVGGGVVGDVSGFVAATYLRGIPFVQVPTTLLAMIDASIGGKTGVNTAAGKNLIGAF
                     HQPKLVLADLDTLDSLPAAQYAAGLAEAVKHGVIADADYFAFLERDSDAVTARKDAAL
                     ERLVARSVEIKAGIVAADEREAGIRAALNFGHTVGHAVEAASGYSLLHGEAVAIGMAY
                     ESRLAETLGIAESGTAERLARVLVTYRLPVTRPAAASVDQLISKMRNDKKSRSGDLRF
                     SLPRSIGAMHGGVASGWTVATQESVIRDLLAVKQ"
     gene            2985..3878
                     /locus_tag="VFU75_08920"
     CDS             2985..3878
                     /locus_tag="VFU75_08920"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HEU5467000.1"
                     /translation="MARSSTGKAKAFFRSTPSGAFDQYLQDIQKLPLIKDPEEERRLA
                     RRVQKGDERAAERLVTANLRFVISYVKKYQGHGLDLSELVAIGNEGLLKAVKKFDPDQ
                     GVKFISYAVWWVRQAVLKALAEQTRSVRIPLNQNSQLIRMSRTETFLSQELGREPTDN
                     EIATALEDTVENVRTARRMTAAELSLDAPVDRSDKDAATLGERFAGIEGGEIEDSTDG
                     KLMREFIDRIFQKYLAPRERKILYLYYGLEEGSEAMTLEKIGALMGVTRERIRQIRER
                     AFEKLRESPDGRALKGFWRAA"
     gene            4007..5416
                     /locus_tag="VFU75_08925"
     CDS             4007..5416
                     /locus_tag="VFU75_08925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HEU5467001.1"
                     /translation="MTPELAEELASIVGASHVRTSRAERLTYAADGLPTHRTVPGIVV
                     LPGTREELIAVVRLLATRNVPFVPRGAGTGLSGGALASEDAVLLVLTRLNRIVSVEPM
                     NRRAIVEPGVVNLKLSAAVLPMGLHYAPDPSSQSACTVGGNVAENAGGPHCLKYGVTT
                     NHILALEVVLADGSVVELGSPAGDAWGPDLVGGFVGSEGNFGVATRITVRLTPLPRTI
                     RTLLADFLSLRAAGEAVSGIIASGVVPAALEMMDQSCIGAVEDSVYAAGYPRDAAAVL
                     LVELDGRFESAVVADAEAVARLLTSHGARSVKTAADPAERERLWQGRKKAFGAMGRLS
                     RDLVVQDAVVPRTALPDVLEAIGAIAARYRLRVSNVFHAGDGNLHPNISFDATNPDEH
                     DRVQKASAEIMSTCIASGGTITGEHGVGVDKLRYMPAIFDGESLAAMHAVRRVFDPHE
                     RVNPGKVVPLHACREWSAA"
     gene            5413..6624
                     /locus_tag="VFU75_08930"
     CDS             5413..6624
                     /locus_tag="VFU75_08930"
                     /inference="COORDINATES: protein motif:HMM:NF013714.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEU5467002.1"
                     /translation="MSLIEKITTLLGQDAVLERRSGDEPPRVAPAAPDAVALLLATAN
                     AEGWKVRVEGAGTWMPGDAPADLILTTRRLDKIPALESQDLTVTAESGVGLDVLRREL
                     ADRGAWLALDPPGLAGRTLGSVVATASAGPLRLGYGSVRDHLLGLAFVTGDGRLIQSG
                     GKVMKNVAGYDLTRLETGAFGAFGVIVLAHLRLRTLPRADVTWVLEGSREDLTNAVDD
                     IHAAVLEPAALELLSPAPARRERWMLAVRLVGSPPHVAAAEQELRIAGGGRLAPLRGE
                     EAQRFWQHAAEGFATRPVVFRVGGLPDGSDGLLDLLQHQVGDDWVLASPTTGTARWAG
                     DTSADRLIKLRRTLAAREVPLTLERAPWSVRSVVGHFGAYREGVGPLVAGLRKTFDPN
                     GVLVQAAEATE"
     gene            6621..7874
                     /locus_tag="VFU75_08935"
     CDS             6621..7874
                     /locus_tag="VFU75_08935"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HEU5467003.1"
                     /translation="MTAADRGGFAALDPCVHCGFCLQACPTFLVTGDEADGPRGRIEL
                     MRSLERGLIAPDDEGLQYHLDRCLGCRGCEPVCPSGVGYGRGLEAARERIAEIRPVPL
                     IARLALWLLTTPGISRIVYLAARLFRATGIPGRLSGTGRIGFAMGMLAATEKRDSGLG
                     IRDSHGADQSPIPNPQSPALLFTGCVMDGLFSHVHAATIRTLRVNGYGTTAPAGQVCC
                     GALHAHAGLVGEARELARRNIEAFGDGDEPIVVNSAGCGALLKDYGHLIPDDPRAARF
                     AARVKDVTEVLADAGPVPATHPELAVAYDPPCHLIHAQRVSDAPLKVLRQISGVKVIL
                     ARDAEKCCGSAGMYTVTEPGLSKDVFAVKVDGLLDASPDLVVTGNPGCIMQIGAGLAA
                     RRVPTPVRHPVEVLDDAYASSGRYG"
     gene            7871..8704
                     /locus_tag="VFU75_08940"
     CDS             7871..8704
                     /locus_tag="VFU75_08940"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5467004.1"
                     /translation="MITELVQTTDGVAVGLHHLEPVAPPTAPVLLLLHGAFSGHTIWL
                     RSFAGQLAIHGFDIWLTDFRGHGVAHREGAPRTWWFDDLIERDAPALIERLRAHIGSR
                     RLGVIGHSAGGAVGLAALARAGAGPRWDALVTLGTPGPSRMGLKRYAGAFGFRTLALA
                     LGRFPARALRFGRDDEGALMFGQWMGWNLQGRWLSRDGFDYLEALSRARTPFLGVAGG
                     RDLLFSPPYACREIVERIGSPTKDFHIAPSLNHPGLVLDPRAATEVVPPVVSWLKRVL
                     Q"
     gene            8701..9738
                     /locus_tag="VFU75_08945"
     CDS             8701..9738
                     /locus_tag="VFU75_08945"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467005.1"
                     /translation="MTDATDLPATLARVGPASVTPAVAQALRAGAVVAAAPAGVFDLA
                     GPGAVACMQGLLTNDIEKPGDGALVYGAVLTPKGMIVSDGWAGRRGEVVRLTTSAEGQ
                     ERTASLLQRSIPPRLAKIADRTSELRVLRLTGPSALALAEAARLRVPHAPGRVELDGE
                     VEVGRATEGAPFTLQWIGVPGSLELLKKRLTTAGAVEGEPAALQLARIAAGWPSLAAE
                     VDDKTLPQEVRYDEIGGVSYTKGCYTGQETVSRVHFRGHPNRGLRGIHLAGDPPAEWH
                     TPQPVLLGDKDVGRLTSVAWFPDGPAGSGVWMGLAVLRREVPSAATVRVGMIEGQVTA
                     LPLSLPRFEPA"
     gene            9735..10748
                     /locus_tag="VFU75_08950"
     CDS             9735..10748
                     /locus_tag="VFU75_08950"
                     /inference="COORDINATES: protein motif:HMM:NF013255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEU5467006.1"
                     /translation="MTVLVTGGSGLVGSHVIRALRQEGTPVRALIRPASRHLVAGPGV
                     EVTEGDVTDEQAWRRAARGISGIIHSAALIAQRGTWDDFSRVNVGGTRLAVAAARAAR
                     VRLVHISSVAVYGRGNAYAAGVGGVDERFRFGPIDGHDYYAHSKRESEQVVWDAVGAG
                     VDAVALRPNVIYGEGDRLFSPRVLAAIRLGIVPQVGRGTNHLSCVYAGNVAAAALLAL
                     RTPAAGGLAFNTTDDGPECHTQRGFSDAFADALGIRVRRVRLPYPVARVAVDLWARWL
                     TIRSPGKYPGVGASAVRFLAEENPFVAERARRELGWKPVTSPREAVRRTVTWLLENEK
                     PGM"
     gene            complement(10786..11025)
                     /locus_tag="VFU75_08955"
     CDS             complement(10786..11025)
                     /locus_tag="VFU75_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467007.1"
                     /translation="MKRIALAVALVVLGACAKDNAQNAGGTMAADTTKHMMADTTKKM
                     GDTSAMKMADTSKKMMADTSKKMMAPAAKAPAKKP"
     gene            11151..14108
                     /locus_tag="VFU75_08960"
     CDS             11151..14108
                     /locus_tag="VFU75_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467008.1"
                     /translation="MTDRRTFMGSVAGVAAALRNRLRFPAPAPVIEARPTALYAQPDG
                     HNNLVRVTVTGLDAPAARARVTDRRGQLVGSAGLLPASDGTSLAGEVFVPLSEPTDYQ
                     FEVVVGKDRRASRRIRLSPSKRWTLYWLSTNHTDVGYTDLQERCLEIHRKNLDAALAR
                     LTRDPDYRWSAECALQIISYAENRAPEQTQALVQALRDGKIGFQALFANMLTGILDHE
                     TAARMVWPAGVFARENGLGFVSAQITDVPGQVPTFPMILAASGVKYLASGPNPDRALP
                     LLPESEGTPNGLTGPWTSYPQLYWWEGPDGSRVLHWRNHHYGDGLRYGFNLGALEMGR
                     RLSDWLLTNPVFLSPDWPYDVALLYGVQWDNALVDERMISNMEDFNRRFMYPRIVPGR
                     AEDFFRDVESRYGTRIPVRRGDTGTYWEDGAASTALELALFRRAQLGLRAADLLALWD
                     ERSDQPEDGRPGRIAARAAERRAAWRDALLFGEHTWGAAASIDAPDSRQTVEQWDYKR
                     RFVLGAAATADTQISDGLARLALGQDRGRTRVVLNAGSWNRTDVVRIPGGAGSGWTAG
                     GADVAAVDLEDGDALALIENAPALGYVALRESGHAPRPPADDGNATDAVAGGFKIRLD
                     EATGAVASLVGPDGKERVKASPWAGLNQLVYVTGGERSALWTSGDRRTVANPPVLDVA
                     QATMANVRRQRLPGIGVRLIAERHLKGFPTVTSTVTLYDALPWVDFENRIEKSPTFGK
                     EALYVAFPFAFTQPTVEMEVPLGRMTAERDQQPGACRDWYCHQHWVWLHEGTDGVLWS
                     GPDTPMFTLNDIVRGAWARRIAPDGTLFAYAMNNYWFTNYAARQDGPFTFRFRVSLVP
                     TNDPAEPVRRGWAACDPLYVGPAVDHPSPGSLIPRDSALFIPDTGTMVVGAKRADDLD
                     GVVVKLVDVTGAARSVGVWPAAYVFTAARSVNLVEMNGDALSIGGDHRVPVDLKPWGV
                     AAARLFTPRAPTG"
     gene            14117..15610
                     /locus_tag="VFU75_08965"
     CDS             14117..15610
                     /locus_tag="VFU75_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEU5467009.1"
                     /translation="MITKPATLGELRQTEWASEARRSRRVKDELRENLICLLENGSPL
                     FPGIVGYEETVVPQLVNAILSRHNFILLGLRGQAKSRILRQLVTLLDDEIPILSGSEV
                     NDDPFDPISKYGRELVAEQGDAAPVSWLPRELRYVEKLATPDVTIADMIGDVDPIKAA
                     RGGHLLADELTMHFGLLPRANRGIFAINELPDLAGKIQVGMFNVMQEGDVQLKGYPVR
                     LKLDVAIVFTANPEDYTARGKIITPLKDRIGSEIVTHYPLTVELGVGITGQEAWTDRA
                     GRSVRIPDFLGELVERVAFEARADKRVDKKSGVSQRLPISLLENVVSAAERRAVMCKE
                     KTVVPRVSDVYAALPAITGKLELEYEGELQGPETIAKELIRKAAGATFEARVGGANVD
                     DIVHWFDEGGALKISTDERSETCLKGFSVVPGLLDLIDRVGLAGKKDPGTIVSACELV
                     LEGLVAGRRISRSEELGYVRARPIPKEPPFGKGGGSGLGGGGPNLFQ"
     gene            15661..16587
                     /locus_tag="VFU75_08970"
     CDS             15661..16587
                     /locus_tag="VFU75_08970"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEU5467010.1"
                     /translation="MGTMILGLLLLLAAFFVRLGASLVKSDSGAGRPIGGILKLTSLG
                     LAVAGVLVVVGAANVVIDPGQVGVQHAFGTVSSSPLMPGIRIVPPWASVERFSSREEQ
                     WPPAGQQVEEIEALSSEQMGMHVEVSIRWQIDPMQAPRIYTEIGDERQIQGAVLNAIR
                     KGVRDGMVQYSINDIAKRTQIAQTMEALVDSALITQPRSGSGPFRIATVTAFFLRDLQ
                     PPPAVVQAINAKIAQEQQVETERHRVEVARLQAEQVRLLNVTLTPEALTKQYLEVLRD
                     MKGSDNLIIIVPTEGGLPVLNLGDLRKNLGRH"
     gene            16584..17237
                     /locus_tag="VFU75_08975"
     CDS             16584..17237
                     /locus_tag="VFU75_08975"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HEU5467011.1"
                     /translation="MTENPPLITITRQYAAGGSEVAKHVAAELGWTVIDNEFVDEVAR
                     RAGLPPDEVAQKEERAPGLLARIARNLAAASPEISAGTRAGTTALPEAEAEEATIVKI
                     TERVITEAAMHGRAVLVGRGAQAVLARRPNALHVYLVGPKAWRMEMAVEHLGVAREDV
                     EKVLDETDRDRDRYVKAHYGRWRQEATLYDMVLNTQRLGFRGAGDVVVAEARRRQWK"
     gene            complement(17241..18464)
                     /locus_tag="VFU75_08980"
     CDS             complement(17241..18464)
                     /locus_tag="VFU75_08980"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5467012.1"
                     /translation="MSEGALRFRLGALAHRNFQLFFLGQGVSLVGTWMQSVALGWLVL
                     ELTNSPFLVGLNSALRSLGVLLFTLYAGIVVDRVDKRRLIVLTQLLQMVEGLILAALV
                     WTHTVAVWQVMALAAFVGVVNAFDIPGRQAFMVDLVGKEDLVNAIALNSSLFNATRIV
                     GPAIAGVIIGWRGVGLCFFLNGVSYIAVIWGLLAMRLPKFVEAPSSESEWQRFRGGLH
                     FILGDRRMSALVLQVAILSLFGFPVLVMMPVFARDVLHTQAGGYGALMAAVGLGAMLG
                     ALALALVSDRVPKGRTLLGGGVAFSIFLSFFALSPWFSVSLVLLALTGCAMIVTTALT
                     NTLIQTFVPDELRGRVMGFYAFMFVGMAPLGAFQAGWLSEHIGAPFATAAGGVVCLAA
                     MLISWWRVPALRETY"
     gene            18506..>19425
                     /locus_tag="VFU75_08985"
     CDS             18506..>19425
                     /locus_tag="VFU75_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467013.1"
                     /translation="MRYTTYQKYLPELADTINLQGLLDQLADFLLQSGFAGGQQHPYW
                     GELSDGESDRSLDALRQAILQALMDSGQLTPEMLQLLRGESTGDADRDADLERQLSEL
                     LDKIVQRLMEEGYLNVSEAPQMPAGQQPMFGPGGMAREAAQQVQFNLTEKGIDFLGYK
                     TLRNLLGSIGKSSFGAHETEHLATGIEAEAASKQYEFGDTLNLDVNATLTRALEREGL
                     GVPINLEYGDLMVHQAEYRSSSATVLMLDCSHSMVLYGEDRFTPAKKVALALTHLIRT
                     QFPGDTLRVVLFHDTAEEIPIAQLAHAQVGP"
BASE COUNT         3236 a         6623 c         6444 g         3122 t
ORIGIN      
        1 ggcccggctc gaacttcgcc gagggattga tgaagtgcac ggcgtggttg acggcgatcg
       61 aggcctccgc gaccccggtc gcgatgagct tcagcttgcc gggataggtg gccatgtcgc
      121 cggcggcgta gatgccggga acgttggtgg acatggtctg gctcaccttg atctcgtgct
      181 tctcgatctc caggccccac tcggcaatcg cgccgaggga cgaaacgaac cccagctgag
      241 gcaggacaca gtccatggcg tgggtctctt ccgccttggt ctggttgttg tagaggacca
      301 ccgactcgat ctgcccgcca ccctgaatgg ccttcagctc ccagaacgtg cgcagctcga
      361 cctgtttcga cgcgtgcatc tgctgcacct gatcgacggt cgcctgatga gcccggaacc
      421 cgtcccggcg atgaatcatc aggatcgact tggcgatggg atgcaggttc acggtccagt
      481 cgaacgccga gtcccccccg cccacgatca gcacccgctt gtcctggaag accttggggt
      541 cgagcacccg gtcatggagg cccctcccga gccactgatc tgcgtccttg agaggaagct
      601 tgcgcggcgt gaacgcaccg atcccaccgg cgatgatcac ggtgcgcgaa ggaaactggt
      661 cctggtccgt agtaatgatg aagagaggcc ggccgtcatc ctctttcttc aggccgacga
      721 ccttctgccc caggtgcacc ggagcccgga actgcgccgc ctgctcggcc aggcccttga
      781 ccaggtcctt ggcgaggatc ttcgggaagc ccgcgacgtc gaagatgtat ttctcaggat
      841 agagcgcggc cagctggcca cccacctggt ccaggttgtc gatgatccgg cagctggcac
      901 cgcgcatccc ggcgtagaac gcgccgaaga gtccggtggg tccggcaccg atgatcgtga
      961 tgtccacgat gtcttgcatg ggcccaacgg taacgggaga gtcgtcaagc gagcaagacg
     1021 gccgtcacgc cgcttctcgc gcggggcctg tggactcaat aggttccacc ccgtgaagat
     1081 ctacacgaag accggagacg cgggcgatac gggcctgttc ggagggggtc gagtgcccaa
     1141 ggaccacccg cgcgtcgcgg cctatggtga ggtcgactcg ctgaacgccg ccgtcggatt
     1201 cgccgcgtcc ctggagccgg ccacctggga aggcgcgctt ctggaaggga ttcagcggga
     1261 tctctttacc acgggcgcca tcctggcgac ccccgatccc acgaagccga aaaccccgcc
     1321 ggccccgatc acggctgagc ggatcaccgc cctggaacag gcgatcgacc ggctggagca
     1381 tgggctccca cccctcgatc gtttcattct tcccggcggc ggacccaagg gagcggcgtt
     1441 ccacgtcgcc cggacgacgt gccgccgggc cgagcgcgcg gtggttaccc tcgcccggag
     1501 cgagacgatt ccgaaggaag tcctcatcta cctgaatcgc ctttccgacc tcctcttcgt
     1561 cctggcccgt tcagccaatc aggccgcggg acaccccgag ctccagtggt aacagtccgt
     1621 gtcgcactct ccgccgagag cggaggggaa cgcagcgcgt cttacgacgt gctggtgggc
     1681 cggggcctgc tgaaagacct cacgaccctg ctgtcgcgac atgcacccgc atcccgctac
     1741 gtcgtcatca cggacgacca gatcgagcgc acgtttgcgc gcaaagcagc aaacgatctc
     1801 agtgccccac tgctcagttt ccccgccggg gagggccgga aaaccaggga aacctgggcc
     1861 gatctcaccg accggctcct ggctcatcat gtgggccggg acgcctgcat tgtcgcggtg
     1921 ggtggcggcg tggtgggcga cgtctccggc ttcgtcgcgg cgacgtacct gcgcggcatc
     1981 ccattcgttc aggtgccgac gaccctcctg gcgatgatcg acgcatcgat cggcgggaaa
     2041 accggggtca acacggcggc gggaaagaat ctgatcggcg cctttcatca gccgaagctg
     2101 gtcctcgccg acctcgacac cctcgattcg ttgccggcgg cgcagtacgc cgcggggttg
     2161 gcggaggcgg tgaagcacgg ggtgatcgcc gacgcggatt atttcgcatt tctcgagcgg
     2221 gacagtgatg ccgtgacggc gcggaaggat gccgcgctcg aacgtctcgt cgctcgctcc
     2281 gtggagatca aagccggcat cgtggccgcc gacgagcggg aagccgggat ccgcgccgcg
     2341 ttgaatttcg gacataccgt gggtcacgcg gtggaggccg cgagtggtta ctcgctgctc
     2401 cacggtgaag cggttgccat cgggatggcc tacgagtcgc gactcgcgga aaccctcggc
     2461 atcgcggagt cgggaacggc cgaacggctc gcccgcgtgc tcgtgaccta tcgcttgccc
     2521 gtcactcggc ccgctgccgc gtcagtagat cagctgattt cgaaaatgcg gaacgacaag
     2581 aagtcacgat cgggagatct gcgcttttcg cttccgcgtt cgatcggcgc gatgcacggc
     2641 ggcgtcgcga gtgggtggac ggtggcgacc caggagagcg tgatccgcga cctcctggcg
     2701 gtcaaacagt aatcacatat ctctcaacca cttcccacac gctttccacc tctccacaca
     2761 gccgcttccg gcgcattcaa atgatttctc cacaacactt gttgatagtg gacaaacctc
     2821 gcagtgaacc gtagaagcaa caacttgtgg tgtggaaaac tgcgggaatt tgcaccattc
     2881 tgggtcaagt cctatattgg ccagcgtttc cagaaggctc tggacgagtg tttggtgcac
     2941 cgtccccctc aaaaacgtca cagccgaaga tgggagtgcc gtgcatggcc cggtcgtcca
     3001 caggcaaggc aaaggcgttc ttccgctcta ctccgtctgg cgccttcgac cagtatctgc
     3061 aggacattca gaaactcccg ttgatcaaag accccgagga agagcgccgg ctggcccgac
     3121 gcgtacaaaa aggcgatgag cgtgccgccg aacggctggt cacggccaac ctccggttcg
     3181 tcatctctta cgtgaagaag taccagggac acggactcga tctctccgag ctcgtggcca
     3241 tcggcaatga ggggctcctc aaggctgtca agaaattcga tcccgatcag ggcgtgaagt
     3301 tcatttcgta cgcggtatgg tgggtccggc aagccgtgct gaaggctctt gccgaacaga
     3361 cccgcagcgt tcggatcccc ctgaatcaga attctcagct catccgcatg agccgcaccg
     3421 agaccttcct gtcccaggag ctcggccgcg agcccaccga caacgaaatc gccacggcgc
     3481 tcgaagacac agttgagaac gtgcggaccg cgcgccggat gaccgcggcc gagctttcgc
     3541 tcgacgcccc ggtggaccgc agcgacaagg acgcggcaac gttgggtgag cggttcgccg
     3601 gcatcgaagg cggcgagatc gaagacagca ccgacgggaa gctcatgcgg gagttcatcg
     3661 accgcatctt ccagaagtac ctggctcccc gcgaacgcaa gattctctat ctctactatg
     3721 gactcgagga aggctctgag gcgatgaccc tcgagaagat cggcgcgctc atgggagtga
     3781 cccgtgagag aatccgccaa atccgcgagc gggcgtttga aaagctgcgc gagtcgcctg
     3841 acggcagggc cctgaagggg ttctggcgcg cagcttagct cacacggttt gacggatcac
     3901 cgggggccag ggcggaatcc tggccccctt ccgtttgccc tcctgacccc cacaccccgc
     3961 tatgcgtctg tgcgtctagc cgtgcttttc ctgtatactt gatttcgtga cccccgaatt
     4021 ggccgaggaa ctcgcttcga tcgtcggcgc cagtcacgtc cgcacctcgc gggccgaacg
     4081 gctgacctac gccgccgacg gcctccccac ccaccgcacc gttcccggca tcgtcgtgct
     4141 ccccggaacc cgcgaggaac tcatcgccgt cgtcaggctc ctcgccaccc ggaatgtccc
     4201 cttcgtgccc cgcggagcgg gcaccggact ctccggggga gcgctcgcct cggaagacgc
     4261 cgttctcctg gtgctcaccc ggctcaaccg gatcgtatcg gtggaaccca tgaaccgtcg
     4321 cgcgatcgtc gagccgggtg tcgtcaacct caagctctcg gccgccgtcc tccccatggg
     4381 actccactac gcccccgacc cttccagtca atcagcctgc acggtcggcg gaaacgtcgc
     4441 cgagaatgcc gggggcccgc attgtctcaa gtacggggtc acgaccaatc acatcctcgc
     4501 cctggaagtc gtactcgctg acggctccgt ggtggagctg ggctctccgg ccggtgacgc
     4561 ctggggcccc gacctcgtcg gcggattcgt gggctccgaa ggcaacttcg gcgtagccac
     4621 ccgcattacc gtcagactca cccccctgcc tcgcaccatc cgcacgctgt tggccgactt
     4681 cctctccctt cgcgccgccg gggaggccgt ctcgggaatc atcgcgagtg gcgtcgtgcc
     4741 ggccgcgctc gagatgatgg atcagtcgtg catcggcgcg gtcgaggact ccgtctacgc
     4801 cgccggctat ccgcgcgatg cggccgcggt actcctggtc gagttggacg ggcgcttcga
     4861 gtcggcggtc gtcgcggatg ccgaggcggt ggcccgcctc ctgacctccc acggcgccag
     4921 gagcgtgaag acggccgcgg atcccgccga acgcgagcgg ctctggcagg gccgaaagaa
     4981 ggcgttcggg gccatgggcc ggctctcccg cgacctcgtg gtgcaggacg ccgtggtccc
     5041 ccggaccgca ttgccggacg ttctcgaagc gatcggcgcc attgcggccc ggtaccggct
     5101 acgcgtgagc aacgtcttcc atgccgggga cgggaacctc caccccaata tttctttcga
     5161 cgccaccaat cccgacgagc acgatcgggt ccaaaaagcg tcggccgaga tcatgagcac
     5221 gtgcatcgcg tcagggggca cgattacggg cgaacacggc gtgggggtgg acaagctccg
     5281 gtacatgccc gccatcttcg atggtgaatc gctggcggcg atgcatgcgg tccgccgggt
     5341 gttcgacccc cacgaacggg tgaaccccgg caaagtggtg cccttgcacg cgtgccggga
     5401 atggagcgcc gcgtgagcct catcgagaag atcacgaccc tgctgggtca ggacgccgtg
     5461 ctcgagcgcc gctcgggaga cgagccgccc cgggtcgcgc cagcggctcc cgatgccgtc
     5521 gcgctgctcc tcgccaccgc aaatgccgaa ggatggaagg tccgggtgga gggcgcgggg
     5581 acctggatgc ccggggatgc cccagccgac ctcattctca ccacccgccg gctcgacaag
     5641 atcccggcgc tcgaaagcca ggacctcacc gtcaccgcgg agtcgggagt cgggctggac
     5701 gtgttgcgcc gggagctcgc cgatcgcggc gcctggctcg ccctcgatcc accgggactg
     5761 gccggcagga cgctgggctc cgtcgtggcc accgccagcg ccggtcccct ccggctcggt
     5821 tacggcagcg tccgcgacca cctcctcggc ctcgcgttcg tgaccgggga cggccggctg
     5881 atccagagtg gcggcaaggt gatgaagaac gtcgccgggt acgacctgac tcgactggag
     5941 accggggcct tcggagcctt cggggtcatc gtcctggcac acctccgcct gcgcaccctg
     6001 ccgagggccg acgtcacctg ggtcctggag ggctcgcgtg aggacctcac caacgccgtg
     6061 gatgacatcc acgcagcggt cctggagccg gcggctctcg agctgctctc gccggcgccg
     6121 gcgcgtcgcg aacgctggat gctggcggtc cgcctcgtgg gctccccgcc gcacgtcgcc
     6181 gcagccgagc aggaattgcg gatcgcgggc ggtgggcggc tcgcgcccct ccggggcgag
     6241 gaagcccagc gattctggca gcatgccgcc gagggattcg caacgaggcc ggtggtgttc
     6301 cgggtcggtg gactccccga cggctcggac gggctgctcg acctgctcca gcatcaagtg
     6361 ggagatgact gggtgctcgc gagtcccacc accggcaccg cgcgttgggc aggggatacg
     6421 agcgcggacc ggctgatcaa gctccgccgc acgcttgccg cccgtgaagt gccgctcacc
     6481 ctcgagcggg ccccgtggtc ggtgcggtcc gtcgtgggac acttcggcgc gtaccgcgag
     6541 ggcgtgggtc cgctggtcgc ggggttgcgg aaaaccttcg accccaacgg ggtcctggtg
     6601 caggccgccg aagccacgga atgacggcag ccgatcgcgg gggcttcgcc gcattggacc
     6661 cgtgcgtcca ctgcgggttc tgccttcagg cctgccccac gtttctcgtc accggcgacg
     6721 aagccgacgg gccccgcggc cgcatcgagc tgatgcgatc cctggagcgc ggcctcatcg
     6781 cacccgatga cgaaggactc caataccacc tcgaccgctg cctcggctgc cgcggctgtg
     6841 agccggtctg tccttcagga gtcggctacg gccggggcct cgaagccgcg cgcgaacgaa
     6901 tcgccgagat tcggcccgtt cccttgatcg cccgtctcgc actctggctc ctcaccactc
     6961 cgggtatttc gcggatcgtc tacctcgccg cgcggctctt tcgagcgacc gggatccccg
     7021 gccggctgtc gggaacagga cggattggtt tcgcgatggg gatgctggca gcaacggaga
     7081 agcgggattc gggattgggg attcgggatt cgcacggcgc cgaccaatcc cccatcccga
     7141 atccccaatc cccggcgctg ttgttcaccg gctgcgtaat ggacggcctg ttctctcacg
     7201 tccacgccgc caccatccgc accctccgcg tgaacggcta cgggaccacc gccccggccg
     7261 ggcaggtctg ttgcggagcc ctgcacgcgc acgcggggtt ggtgggggag gcccgggagt
     7321 tggctcgtcg caatatcgag gcgttcggcg acggcgacga gccgattgtc gtgaatagcg
     7381 cgggctgtgg ggcattgctg aaggattatg ggcacctgat acccgacgat cccagagccg
     7441 ctcgcttcgc ggcgcgcgtc aaggacgtga ccgaggtgtt ggcggatgcc gggccggtcc
     7501 cggccaccca tcctgagctc gcagtggcct acgatcctcc gtgccacctc atccacgctc
     7561 agcgggtgag cgacgcgccg ctgaaagtgc ttcggcagat ctccggcgtg aaagtgatcc
     7621 tcgcgcggga cgccgagaag tgctgcggga gtgccggcat gtacacggtt accgagccgg
     7681 gactgtcgaa ggacgtcttc gcggtaaagg tggacggact cctcgacgcc tcgcccgacc
     7741 tggtggtcac gggcaacccg ggctgcatca tgcagatcgg cgccgggctc gccgcccggc
     7801 gggtcccgac acccgtccgc catcccgtcg aagtgctcga cgatgcgtac gcatcttcag
     7861 gccggtacgg gtgatcacgg agctggtcca gaccaccgac ggcgtcgcgg tgggcctcca
     7921 tcatctcgag ccggtcgcgc ctcccaccgc cccggtcctg ctcctcctgc acggtgcgtt
     7981 ctccggtcac accatctggc tgcggagctt cgcgggacag ctcgccatcc acgggttcga
     8041 tatttggctc accgacttcc gcggccatgg tgtcgcgcac cgcgaagggg cacccaggac
     8101 ctggtggttc gacgacctca tcgagcgcga cgccccggcg ctgatcgagc ggcttcgcgc
     8161 gcacatcggg tcccgccggc tgggggtgat cggacattcc gcgggtggcg ccgtcggcct
     8221 cgccgcgctc gcccgggcgg gagcaggacc gcgatgggat gcgctcgtca ccctgggaac
     8281 cccgggcccg agccgcatgg ggctcaagcg gtacgccggt gcgttcgggt tccgcaccct
     8341 ggcgctcgcg ctcggacgct ttcccgcgcg ggcgctgcgc ttcggcaggg acgacgaggg
     8401 cgcgctgatg ttcggtcaat ggatggggtg gaatctccag gggcgctggc tctcgcggga
     8461 cggcttcgac taccttgagg cactgtctcg ggctcgcact ccctttctgg gcgtcgcggg
     8521 cgggcgagac ctgctcttct cgccgccgta cgcctgccgg gaaatcgtcg agcggatcgg
     8581 atctccgacg aaggattttc acatcgcgcc ctcgctgaat caccccggcc tggtactcga
     8641 cccccgtgcg gcgacggagg tcgtaccccc ggtcgtgtcc tggctcaaga gggtgctgca
     8701 atgacggacg ccacggacct gccggccacc ctcgcccggg tcgggccggc atcggtcact
     8761 cccgcggtgg cccaagccct ccgcgccggg gcggtcgtcg ctgccgcgcc tgccggtgtc
     8821 ttcgacctcg cggggccggg cgcggtcgcc tgcatgcagg gactgctcac caacgacatc
     8881 gagaaaccgg gcgatggcgc gctcgtctat ggagccgtcc tgacgccgaa aggaatgatc
     8941 gtctccgacg ggtgggccgg gcgtcggggg gaggtggtcc gactcacgac ctctgcggag
     9001 gggcaggaac gcaccgccag cctcctgcag cgatccatcc ccccccggct cgcgaagatc
     9061 gccgaccgaa cgtcggagct ccgcgtgctt cgcctgacgg gtccctccgc gctggcgctc
     9121 gccgaagccg cgcgtcttcg tgttccccac gcgcccgggc gcgtcgagct cgacggtgaa
     9181 gtcgaggtcg gccgtgcgac ggaaggcgcg cccttcacgc tccaatggat cggcgttccc
     9241 gggtcactcg agctgttgaa gaagcggctg acgacggccg gcgccgtcga aggagagcca
     9301 gccgccctgc agctcgcgcg tatcgcggcg gggtggccgt cgctcgccgc ggaggtggac
     9361 gacaagaccc tccctcagga agtgcgatac gacgagatcg gtggcgtctc ctacacgaag
     9421 ggctgctata ccgggcagga gacggtctcg cgcgtccact ttcgcgggca tccgaatcgc
     9481 ggcctccgcg gcatccacct cgccggcgac ccgcccgcgg aatggcacac gccacagccg
     9541 gtccttcttg gcgacaagga cgtgggtcga ctcaccagtg tcgcctggtt tcccgatggt
     9601 cccgcgggat cgggggtctg gatgggtctc gccgtgctgc gacgggaggt gccctccgcc
     9661 gcaaccgtcc gcgtcggcat gatcgagggc caggtgacgg ctcttccact ttccttgcct
     9721 cggttcgagc cggcgtgacg gttctcgtca ccggcgggag tggattggtc gggagtcacg
     9781 tgattcgagc cctgcgccag gaggggacgc cggttcgcgc cctgatccgg cctgccagcc
     9841 ggcacctcgt ggccggaccg ggcgtcgaag taactgaggg cgacgtgacc gatgagcagg
     9901 cgtggcgtcg cgccgcgcgg ggcatatccg ggatcattca ttcagcggcc ctcatcgcgc
     9961 agcgaggcac gtgggacgac ttctccagag tgaacgtcgg tggcacgcgg ctcgccgtgg
    10021 ccgcggcgcg cgccgcccgg gtgcgcctgg tccacatctc ctcggtggcg gtgtatggcc
    10081 gcggcaacgc atacgccgcc ggcgtgggcg gtgtggatga acggttccgc tttggcccga
    10141 tcgacgggca cgactattac gcccacagca agcgggagtc cgaacaggtg gtgtgggatg
    10201 ccgtaggtgc cggggtcgat gcggtcgctc tccggcccaa cgtcatctac ggcgagggag
    10261 accgcctctt ttccccgagg gtgctcgcgg ccattcgtct gggcatcgtt ccgcaggtgg
    10321 gccgggggac caaccatctc tcgtgcgtgt acgcaggaaa tgtcgcggcg gccgcgctcc
    10381 tggcactccg gacgccggcc gctggcggat tggccttcaa caccaccgat gatggacctg
    10441 aatgccatac ccaacgggga ttctccgacg ccttcgcgga tgcactcggc attcgggtac
    10501 ggcgcgtgag gctcccctat ccagtggcgc gcgtcgcggt ggatctctgg gctcgctggt
    10561 tgacgatccg cagtcccggc aagtatcccg gcgtgggcgc ctccgcggtg cgcttcctgg
    10621 cggaggagaa tccctttgtc gcggagcggg cgcggcggga gttgggctgg aagccggtca
    10681 cttcaccacg cgaggcagtg aggcgcaccg tgacctggct tctggaaaac gaaaagcccg
    10741 ggatgtaagg cccgggcttt gatcgtcaac gggacggcga gaagcttacg gcttcttggc
    10801 cggagccttg gcggccggcg ccatcatctt cttcgacgta tcggccatca tcttcttgga
    10861 ggtgtcggcc atcttcatgg cggacgtatc acccatcttc ttggtcgtgt cggccatcat
    10921 gtgcttcgtc gtgtcggcgg ccatggtgcc accggcgttc tgcgcgttgt ccttcgcgca
    10981 ggcaccgagg accacgaggg ccacggcgag tgcgatacgc ttcattacgt actcctccga
    11041 tataggggcg agtggttctt ccggcgcggg gaaaaggtag cattgaccag atacccgtca
    11101 aggccgcgca cgttcacgac ggatgcaaga ccacacaagg gatgcgatgt atgactgatc
    11161 gccgaacgtt catgggctcc gtcgcaggcg tggcggcggc gctgcgaaat cggctccgat
    11221 ttccggcgcc cgcgcccgtc atcgaggctc gcccgacggc gctctacgcc cagcccgacg
    11281 gtcacaacaa ccttgtccgg gtcaccgtca cggggctcga tgcccccgcc gcgcgcgccc
    11341 gggtcaccga tcgccgcggc cagctcgtgg ggtcggccgg gctgctgccg gcgtcggacg
    11401 gcacttcgct cgccggtgaa gtgttcgttc cgctctccga acccaccgac taccagttcg
    11461 aagtcgtcgt cggaaaggat cgccgcgcga gccgccgcat ccggctgtcg ccgtcgaaac
    11521 gctggaccct ctactggctc tccacgaatc acacggacgt cgggtacacc gacctccagg
    11581 agcgatgtct cgagattcat cgcaagaatc tcgacgcggc gctcgcccgc ctgacccggg
    11641 atccggatta ccgttggagc gccgagtgcg ccctgcagat catttcgtat gcggagaacc
    11701 gcgcgccgga gcagacccag gcgctggtcc aggcactccg ggacgggaag atcggtttcc
    11761 aggcgctgtt cgcgaacatg ctcaccggca tcctcgatca cgagaccgcg gcgcgcatgg
    11821 tctggcccgc cggcgtcttc gcccgcgaga acggcctcgg cttcgtctcg gcacagatca
    11881 ccgacgtccc cggtcaggtc ccgacgttcc ccatgatcct ggccgcgagc ggtgtgaagt
    11941 atctcgcgtc gggccccaac cccgatcgag ccttgcctct cttgcccgaa tcggagggca
    12001 cccccaacgg gctgaccgga ccctggacct cctatcccca actgtactgg tgggagggcc
    12061 ccgacggctc ccgggtgctc cactggcgca accaccacta cggggacggg ctccgctacg
    12121 gcttcaacct cggcgcgctc gagatgggcc ggcggctctc ggattggctg ctcacgaacc
    12181 cggtcttcct ctcgcccgat tggccgtacg acgtcgccct cctgtatgga gtgcagtggg
    12241 acaacgcgtt ggtggacgag cggatgatct cgaacatgga ggacttcaac cggcggttca
    12301 tgtatccgcg gatcgtcccg ggacgcgccg aagatttttt ccgcgacgtc gaaagccgct
    12361 atggcacccg gattccggta cgccgaggag atacagggac gtattgggag gacggcgcgg
    12421 catcgaccgc gctcgagctt gcgctgttcc ggcgggctca gcttgggctc cgcgccgccg
    12481 accttctggc gctctgggac gagaggtcag accaaccgga agacggacgt cccggacgga
    12541 tcgcggcacg ggccgccgag cggcgggccg cctggcgaga cgccctcctg ttcggcgagc
    12601 acacgtgggg cgcggcggcg agcatcgacg cacccgatag ccggcagacc gtcgagcagt
    12661 gggactacaa acgccggttc gtgctgggtg ccgccgcgac cgctgacacg cagatctcgg
    12721 acggtctggc ccggctggcc ctcggacaag accgcgggcg cacgcgcgtc gtgctgaacg
    12781 ccgggagctg gaaccgcacc gacgtcgtcc gaattcccgg tggcgccggc tccggatgga
    12841 cggccggtgg cgccgatgtg gccgcggtgg acctcgagga cggggacgct ctcgctctga
    12901 tagagaatgc gcccgcgctt ggttacgtcg ccctgcggga atcggggcac gcgccacgcc
    12961 cgcccgcgga cgacgggaat gcgacggacg ccgtggcggg cggattcaag atccgcctgg
    13021 acgaggcgac gggggccgtc gcgtccctcg tgggacccga tggcaaggag cgggtgaagg
    13081 catcgccgtg ggccggactg aatcagctcg tgtacgtcac gggcggcgag agatcggcac
    13141 tctggacatc cggagaccgc cggacggtcg ccaatccgcc cgtcctcgac gttgcgcagg
    13201 ccacgatggc gaacgtgcgg cggcagcggc tgccgggcat cggcgtgcgg ctgatcgccg
    13261 agcggcacct caagggattc ccgacggtga cgtcgaccgt caccctctac gatgctctcc
    13321 cgtgggtgga cttcgagaac cgaatcgaga agagtcccac attcgggaaa gaggcgctct
    13381 acgtcgcctt cccgttcgca ttcacccaac ccaccgtgga aatggaagtg ccgctgggcc
    13441 ggatgaccgc cgagcgcgac cagcagccgg gtgcgtgccg cgactggtac tgccaccagc
    13501 actgggtctg gctccacgaa gggaccgatg gcgtactgtg gagcggcccc gacaccccga
    13561 tgttcacgct gaacgacatc gtccggggcg cctgggcccg ccgcatcgcg ccggacggga
    13621 cgctcttcgc gtacgcgatg aacaactact ggttcacgaa ctacgccgcc cgtcaggacg
    13681 gtccgttcac gttccggttc cgggtgtcac tggttccgac caacgatccg gcggagccgg
    13741 tccggcgggg ctgggctgcg tgcgatccgt tgtatgtcgg acccgccgtg gatcatccct
    13801 cccccggctc gctcatcccg cgggactccg ccctgttcat tcccgacacg ggaacgatgg
    13861 tggtcggcgc aaagcgcgcg gacgacctcg acggcgtggt ggtcaagctg gtggacgtaa
    13921 cgggagcggc acggagtgta ggggtgtggc cggccgccta tgtgttcacc gcggcccgga
    13981 gcgtcaacct cgtggagatg aacggggacg cgctctccat cgggggagat catcgcgtgc
    14041 ccgtggacct caaaccgtgg ggagtggcgg ccgcacggct ctttacaccg cgcgcgccga
    14101 ccggttaacg tcggacatga tcaccaaacc cgccaccttg ggtgagctgc gccagaccga
    14161 gtgggccagc gaagcccgtc gatcccgcag ggtcaaggat gagctgcggg aaaacctgat
    14221 ctgcctgctc gagaacgggt ccccgctctt ccccggcatc gtcggctacg aagagacggt
    14281 ggtgccgcag ctcgtgaatg ccattctctc ccggcacaac ttcattctcc tcggactccg
    14341 cgggcaggcg aagagccgga tcctccggca gctcgtgacg cttctggatg acgagattcc
    14401 tatcctgtcg ggctcggaag tgaacgacga ccccttcgac ccgatttcga agtacgggcg
    14461 cgagctggtg gccgaacaag gcgacgcggc gccggtcagc tggctgccgc gcgagctccg
    14521 gtacgtcgag aagctcgcca caccggacgt caccatcgcg gacatgatcg gcgacgtgga
    14581 tccgatcaag gccgcccggg gcgggcacct gctcgccgac gagctcacca tgcacttcgg
    14641 cctcctgccc cgcgcgaacc gcgggatctt cgcgatcaac gagctcccgg acctggccgg
    14701 caagatccag gtcgggatgt tcaacgtgat gcaggagggg gacgtccagc tgaagggcta
    14761 cccggtccgg ctcaagctcg atgtcgcgat cgtcttcacg gcgaaccccg aggattacac
    14821 ggcccgcggc aagatcatca cgccgctcaa ggaccgcatc ggctccgaga tcgtcacgca
    14881 ctacccgctc acggtagagc tgggggtcgg catcacgggc caggaggcct ggaccgaccg
    14941 ggccgggcgc agcgtgcgga ttcccgactt cctcggcgag ctggtcgagc gcgtggcctt
    15001 cgaagcccgg gccgacaagc gggtcgacaa gaagagcggc gtctctcagc ggctccccat
    15061 ctcgctcctc gagaacgtgg tctccgccgc cgagcggcgc gccgtcatgt gcaaggagaa
    15121 gacggtcgtg ccgcgggtct cggacgtgta cgccgcgctg cccgcgatca cgggcaagct
    15181 cgagctcgag tacgaaggcg agcttcaggg ccccgagacc atcgcgaagg agctgatccg
    15241 caaggccgcg ggcgcgacgt tcgaggcgcg cgtcgggggc gccaacgtcg acgacatcgt
    15301 gcactggttc gacgaagggg gcgccctcaa gatctccacc gacgagcgct cggagacctg
    15361 cctcaagggc ttcagcgtcg tcccgggtct gctcgacctc atcgaccgcg tggggctggc
    15421 cggaaagaag gaccccggga cgatcgtctc ggcctgcgag ctggtcctcg aaggactcgt
    15481 cgcgggccgg cgcatctcgc gctcggagga gctcggctac gtccgcgccc gtccgattcc
    15541 caaggaaccg ccgttcggca agggcggcgg gagcggcctc ggtgggggag ggccaaacct
    15601 ctttcagtga gtttgctgaa tccccgtttc tagcagccct cccctccttc tggagctgtc
    15661 atgggcacga tgattctggg tcttctgctt ctcctcgccg cgttcttcgt ccgcctcggc
    15721 gcctcgctcg tcaagtccga ttccggcgcc ggccgcccca tcgggggcat cctcaaactc
    15781 acgtcgctcg gactggccgt cgccggagtg ctggtggtcg tcggcgccgc gaacgtcgtg
    15841 atcgatcccg gccaggtcgg agtccagcat gcgttcggca ccgtgtcctc gagccccctc
    15901 atgccgggca tccgcatcgt tcccccctgg gcttcggtgg agagattctc gagccgcgaa
    15961 gagcagtggc cccccgccgg ccagcaggtg gaggagatcg aggcgctctc gtcggagcag
    16021 atgggcatgc acgtcgaggt ctccatccgg tggcagatcg accccatgca ggcgccgcgc
    16081 atctacaccg aaatcggcga tgaacgccag atccagggcg ccgttctgaa cgcaatccgg
    16141 aagggcgtgc gggacgggat ggtccagtat tcgatcaacg acatcgccaa gcgcacccag
    16201 atcgcgcaga ccatggaggc cctcgtcgat tccgcgctca ttacgcagcc ccgctcgggc
    16261 agtggcccgt tccgcattgc cacggtcacc gccttcttcc tccgcgatct ccagccgccg
    16321 ccggcggtcg tccaggcgat caacgcgaag atcgcgcagg agcagcaggt cgaaaccgag
    16381 cgccatcgcg tcgaagtcgc ccgcctgcag gccgagcagg tccggctgct caacgtgacg
    16441 ctgacgcccg aggcgctcac caagcagtat ctcgaggtcc ttcgggacat gaaaggctcc
    16501 gacaacctca tcatcatcgt gccgaccgag ggaggactcc cggtgctcaa cctcggggat
    16561 ctgagaaaga acctgggccg gcattgaccg agaatccgcc gctcatcacg atcacccggc
    16621 agtacgccgc cggcggctcc gaagtcgcga aacacgtcgc ggcggagctc ggctggacgg
    16681 tgatcgacaa cgagttcgtc gacgaggtcg cccggcgggc gggcttgcct cccgacgaag
    16741 tggcgcagaa ggaggagcgg gcgccggggc tcctggcccg gatcgcccgc aacctcgcgg
    16801 ccgcgtctcc tgagatctcg gccggcaccc gggccgggac caccgctctg ccggaggccg
    16861 aggccgaaga ggcgaccatc gtgaagatca cggagcgcgt catcacggag gcggcgatgc
    16921 acgggcgcgc ggtgctcgtc ggccgcggag cgcaggccgt cctcgcccgc cggccgaacg
    16981 cgctgcacgt ctacctcgtc ggacccaagg cgtggcgcat ggagatggcc gtcgaacatc
    17041 tcggcgtcgc gcgcgaggat gtcgagaagg tgctcgacga gaccgaccgg gaccgggacc
    17101 gctacgtgaa ggctcactac gggcggtggc ggcaggaagc cacgctctac gacatggtgc
    17161 tcaatacgca gcgattggga tttcgcggtg ccggtgacgt cgtagtcgct gaagcaagac
    17221 ggaggcagtg gaaataggcg ttagtacgtc tcccgcagcg ccggcacccg ccaccacgag
    17281 atcagcatcg cggccaggca taccaccccg ccggccgccg tcgcaaacgg ggcgccgatg
    17341 tgctccgaga gccagcccgc ctgaaaggcc cccagcggcg ccatcccgac gaacatgaac
    17401 gcgtagaatc ccatcacccg gccacgcagc tcatcaggca cgaacgtctg gatgagcgtg
    17461 ttcgtcagcg cggtcgtcac gatcatggcg cagccggtca gggcgagcag cacgagggag
    17521 accgaaaacc acggcgagag cgcgaaaaat gaaaggaaga tgctgaacgc aaccccgcca
    17581 cccagcagcg tccgcccttt gggcacgcgg tccgagacca gcgccaacgc cagagcaccg
    17641 agcatcgccc cgagccccac cgcggccatc agcgcaccgt acccgcccgc ctgcgtgtgc
    17701 aggacgtcac gcgcgaacac cggcatcatc acgagcaccg gaaatccgaa gagcgaaaga
    17761 atcgcgacct gcagaacgag ggcggacatg cgccggtccc ccagaatgaa gtgcagcccc
    17821 cctcgaaagc gctgccactc cgattcactc gagggcgctt ccacgaactt ggggagccgc
    17881 atggccagga gcccccagat caccgcgatg tagcttacgc cgttcaggaa gaaacagaga
    17941 cccacgcccc gccagccgat gatcacgccc gcgatggccg gaccgacgat gcgggtcgca
    18001 ttgaacagtg acgagttgag ggcgatcgcg ttgacgaggt cctccttccc tacgaggtcc
    18061 accatgaacg cctggcggcc gggaatgtcg aacgcattca cgacgccgac gaacgccgcg
    18121 agcgccatca cctgccagac ggccacggtg tgcgtccaca cgagcgccgc gaggatcagc
    18181 ccttccacca tctgcaacaa ttgcgtcagc acgatcaagc gacgcttgtc gacccgatcc
    18241 accacgatgc cggcatagag cgtgaacagc agcaccccga gtgaccgcag cgcggagttg
    18301 aggccgacga gaaagggcga gttggtgagc tcgagcacca gccaccccag ggcgacggac
    18361 tgcatccagg tccccacgag ggagacccct tgccccagga agaacagctg gaagttgcgg
    18421 tgggctaaag cgccgagacg gaagcgcagt gcgccttcgg acattgcggg aaagctcgcc
    18481 accccctcaa gtttggggta gggcaatgcg ctacacgacc tatcagaagt acctgcccga
    18541 gctggccgac acgatcaacc tccaaggcct gctcgatcag ctggcggatt ttctcctcca
    18601 atccggcttc gccggaggcc agcagcaccc ctactggggt gaactctccg acggcgagtc
    18661 agaccgctcc ctcgacgccc tgcgccaggc catcctccag gccctgatgg actcgggcca
    18721 gctgacgccg gaaatgcttc agctgctgcg cggcgaatcc accggcgacg ccgatcgcga
    18781 cgccgatctg gagcgccagc tctccgagct gctcgacaaa atcgtccagc gtctgatgga
    18841 ggaggggtat ctcaacgtca gcgaagcccc gcaaatgccc gcgggccagc agccgatgtt
    18901 cggaccgggg ggcatggcgc gcgaagcggc gcaacaggtc cagttcaacc ttaccgagaa
    18961 gggtatcgac tttctcggct acaagacgct gcgcaacctc ctcggctcca tcggcaagtc
    19021 cagcttcggg gcccacgaaa ccgagcacct ggcgaccggc atcgaagccg aggccgcgag
    19081 caagcaatac gagttcggcg acaccttgaa cctggacgta aacgccacgc tgacccgggc
    19141 cctcgagcgc gaaggcctcg gcgttcccat caacctcgag tacggggacc tgatggtgca
    19201 tcaggccgag taccggtcgt cgtcggcgac ggtactgatg ctcgactgct cacattcgat
    19261 ggtgctctac ggcgaggacc ggttcacgcc ggccaagaag gtggccctgg cgctcaccca
    19321 tctcatccgg acgcagttcc ccggcgatac cttgcgggtc gtcctcttcc acgacacggc
    19381 ggaggagatt cccatcgccc agctggcgca tgcccaggtc ggtcc
//
LOCUS       DASUBP010000239         4688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_893908, whole genome shotgun sequence.
ACCESSION   DASUBP010000239 DASUBP010000000
VERSION     DASUBP010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893908"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..136
                     /gene="infA"
                     /locus_tag="VFU75_08990"
     CDS             <1..136
                     /gene="infA"
                     /locus_tag="VFU75_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU5467014.1"
                     /translation="GHEVLAYVSGKMRKNYIRILQGDRVAVDLSPYDLTRGRITYRYK
                     "
     gene            complement(267..2372)
                     /locus_tag="VFU75_08995"
     CDS             complement(267..2372)
                     /locus_tag="VFU75_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012801.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HEU5467015.1"
                     /translation="MRHAWLVWWGLTVPATAAAQNADSVPKDTLPAIVVSATRADRPL
                     SRTAAAIATVSRADISAARPTWGLDEALGGVPGVVDQNRYNFSLDQKLSIRGAGARSA
                     FAIRGIKVLVDGIPQTLPDGQGQLTNLDLSEVDHIEVERGSSSALFGNATGGVISIWT
                     DPAPPSRLSEDARVAGGQFDGGRSWAKWSSTTRARVGEGSVMLTASRLSYQGERNHSA
                     ADLRDFNSRLRLPLGEHWSLAAVAAYGDDPRADNPGALTNAELHANQDSAAPTNLSRN
                     AGKAVTQGQGGVTLRRVFDNGGEADATVYGFTRDLSNPQTFAFIRLKRFDYGARFTMA
                     QPFAVGPFAQVLTTGFDFQRTHDDRVNFNYVGASSTADTSRQLDQLEHVTEVGPFVQS
                     AVTLTSRLTVTAGMRYDWVSFQADDRLVDSVKALTNPIYLKDSGRRLMRSFSGSLGAA
                     EALSDGLTVYANAGSSFETPTTTELANRPDTVGGFNPLLQPQRTWNYEIGARGASNEF
                     VRWSAAVFQADVRDELIPFQEPSSPQRVFYRNAGSARHRGIEIGAAASPVRGLSVALT
                     YTYSHFRYTDYRYAVGATNYVLDGRPLAGVPEHWLRALVGVRPWGPKGGWMELETVHA
                     SSQLVNDTLSTRVAGWWRTDLRLGWGGSAGSVRLDPFFAVQNLFNHHYVGSVTVNAAG
                     GRYYEPAPGRNFYVGMEVH"
     gene            complement(2386..3126)
                     /gene="lepB"
                     /locus_tag="VFU75_09000"
     CDS             complement(2386..3126)
                     /gene="lepB"
                     /locus_tag="VFU75_09000"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU5467016.1"
                     /translation="MSPKNAPAPLAPKAGPKTFAEEVKSWAKSIGIALLCWIVIRSYL
                     VEAFRIPSSSMENTLLVGDWLFVNKMIYGGTLEIPLTGIRFLTVKGYAQPHRDGLVVF
                     RSVEDSTPDLDIVKRIIGEAGDTLQMVRDTVYRNGKVLPEPYALHLNPGADADIYKLQ
                     MRRNELPHYVGKNPEKYNPTSHDWGPLVVPADSFWVMGDNRDDSYDSRFWGFLPRSHV
                     EGKPELIYFSMGFSPLRIRWNRFFRHPS"
     gene            complement(3123..4148)
                     /locus_tag="VFU75_09005"
     CDS             complement(3123..4148)
                     /locus_tag="VFU75_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467017.1"
                     /translation="MITFLLAASMFQAASVPARPDEPHLRNLRRLTNGGQNAEAYYSH
                     DGSRLIFQHTPPPDTAGCDQEFIINVDGTGLHRVSDGQGRTTCGWFFAGDQRVFYAST
                     EAVGVTCPPRPDYSHGYVWALYDYDIYASDADGSHKVQLTSSKGYDAEGTLSPDGKTI
                     VFTSERDGDLDIYTMNADGTNVRRLTNTLGYDGGPTFSLDGKLIVYRAYHPSTSADSA
                     EYRTLLAQGVVRPTKMDLWVMNADGSNQRQITHLAGASFAPAFYPGSKRIIFSSNYTD
                     PQSDHFDLYAVDLDGANLETITTDHSFNAFPMFSPDGKHLVWASNRGASVPHETNLFI
                     ADWVEHP"
     gene            4216..4542
                     /locus_tag="VFU75_09010"
     CDS             4216..4542
                     /locus_tag="VFU75_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467018.1"
                     /translation="MTGSPPNPPRKSRVPLLDALGQLCTDGKSTAEYLWQVPDDTQAR
                     LKLVGLLDQIAVESAKQGRKEMGRICADLVPAAKASPSPQQVELLVDGFDRLLKLWQS
                     AKSGLL"
     gene            4560..>4688
                     /locus_tag="VFU75_09015"
     CDS             4560..>4688
                     /locus_tag="VFU75_09015"
                     /inference="COORDINATES: protein motif:HMM:NF012670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467019.1"
                     /translation="MTDVAAGPHTDLDLLCINTVRTLAMDAVQQADSGHPGTPMALA"
BASE COUNT          839 a         1509 c         1532 g          808 t
ORIGIN      
        1 cggccacgaa gtcctggcgt acgtctccgg aaaaatgcgg aagaactaca tccgcatcct
       61 ccaaggggac cgggtcgccg tcgatctctc cccgtatgat ctcaccaggg gcaggattac
      121 ctaccggtac aagtaacaaa aagacgtcaa gcgactgcgt caagcaaatg cgtcgagcga
      181 aaacgtggcg gcctggttac gcagttgctc gacgttgttt gcttgacgct tctgcttgac
      241 gcttctgctt gacgcctttc tgtctgtcag tggacttcca tccccacata gaaattcctt
      301 cccggcgccg gctcatagta ccgcccgccc gccgcgttca cggtgaccga tccgacgtag
      361 tgatgattga agagattctg caccgcgaag aacggatcga gacgcacgct ccccgccgag
      421 cctccccagc ccagacgcag gtcagtccgc caccagcccg cgacgcgtgt gctcagcgtg
      481 tcgttcacca actgactcga cgcatgcacg gtctcgagct ccatccagcc gcccttcggg
      541 ccccagggac gcacccccac gagcgcgcga agccaatgct ccggcacgcc cgccagcgga
      601 cgaccgtcga gcacgtagtt cgtcgccccc acggcgtacc gatagtccgt gtagcggaaa
      661 tgcgaatacg tgtaggtgag ggccacggag agcccgcgca cgggcgacgc ggcggccccg
      721 atctcgatcc cgcgatgacg cgcgctgccg gcgtttcggt agaagacccg ctgcggggac
      781 gacggctcct gaaacggaat cagctcgtca cgtacgtccg cctgaaacac cgccgcgctc
      841 caccgtacga actcgttcga cgctccacga gcaccgattt cgtagttcca ggtgcgctgc
      901 ggctggagca acgggttgaa gccccccacg gtatccgggc gattcgcaag ctccgtggtg
      961 gtcggcgtct cgaacgatga tccggcattc gcgtaaaccg tcagcccgtc cgacagtgcc
     1021 tccgccgcgc cgagcgatcc cgagaacgag cgcatcaggc ggcggccgga gtccttcaga
     1081 tagatggggt tcgtaagggc cttcaccgag tccaccaggc ggtcgtccgc ctggaaactc
     1141 acccagtcgt agcgcatgcc ggcggtgacg gtgaggcggg aagtcagggt gacggcactc
     1201 tgcacgaagg gtcccacctc ggtcacatgc tcgagctggt cgagctgacg tgacgtatcg
     1261 gcggtagagc tcgcaccgac gtagttgaag ttcacgcggt catcgtgcgt ccgctgaaaa
     1321 tcgaagccgg tggtgaggac ctgcgcgaaa ggtccgaccg cgaagggctg cgccatcgtg
     1381 aaccgtgcac cgtagtcgaa ccgcttgagc cggatgaacg cgaacgtctg gggattggag
     1441 agatcacggg tgaacccgta gaccgtggcg tcggcctcgc cgccattgtc gaatacccgc
     1501 cggagcgtca cgcctccctg cccctgcgtc accgccttcc ccgcgttgcg gctcaggttc
     1561 gtcggcgccg cggaatcctg gttcgcgtgc agctcggcat tcgtcagagc accgggatta
     1621 tcggctctcg gatcgtcgcc atatgcggcc acggcggcga gcgaccagtg ctcgcccagc
     1681 gggagccgca gacgcgagtt gaagtcgcgt agatcggccg cgctgtgatt ccgttctccc
     1741 tgataggaga ggcgggatgc cgtgagcatg acactgccct ctcccactcg tgcccgcgtc
     1801 gtggacgacc acttggccca gcttcggccg ccgtcgaact gcccgcctgc cacgcgcgcg
     1861 tcctcggaga ggcgcgacgg gggcgccgga tcggtccaaa tgctgatcac gccgccggta
     1921 gcgttcccga agagggccga cgacgacccg cgttccacct cgatgtggtc cacctcgctg
     1981 aggtccagat tggtgagctg gccctggccg tcgggcagcg tctggggaat tccatcgacc
     2041 agcaccttga tgccacggat cgcgaaagcc gaccgggctc cggcaccccg gatcgagagc
     2101 ttctggtcga gcgagaagtt gtagcggttc tggtcgacga ccccgggaac tccaccgagc
     2161 gcttcatcga ggccccaggt ggggcgggcg gcactgatgt cggcacggct gaccgtcgcg
     2221 atggcagccg cggtccggga gaggggacgg tcggcgcggg tggccgacac gactatcgcg
     2281 ggaagggtgt cctttggaac cgagtctgcg ttttgcgccg ccgccgtcgc cggtaccgtg
     2341 agcccccacc acaccagcca cgcgtgccgc atgccgatga atcggctagg acgggtgccg
     2401 gaagaaccgg ttccagcgga tgcggagggg cgagaacccc atgctgaagt agatcaactc
     2461 cggcttgcct tcgacgtgcg agcgcggcag aaagccccag aaccgggaat cgtaggagtc
     2521 gtcccggttg tctcccatca cccagaagct gtcggcgggg acgaccagcg ggccccaatc
     2581 gtgggacgtc ggattgtact tctcaggatt cttgccgacg tagtgcggga gctcgttccg
     2641 gcgcatctgc agcttgtaga tgtcggcgtc ggcgcccgga ttgaggtgca gcgcgtacgg
     2701 ctccggcagc accttgccat tccggtacac cgtgtcgcgc accatctgca gcgtatctcc
     2761 cgcttcgccg atgattcgct tcacgatatc cagatcgggg gtcgaatcct cgaccgaccg
     2821 gaataccacc aacccgtccc ggtggggctg cgcgtatccc ttgacggtga ggaaacggat
     2881 tcccgtgagc ggaatctcca gcgtgccgcc gtagatcatc ttgttgacga agagccaatc
     2941 ccccaccagc agcgtgttct ccatgctgga cgacgggatt cggaacgcct cgacgaggta
     3001 cgaccggatc acgatccagc acagaagcgc gatgccgatc gacttggccc agctcttcac
     3061 ttcttccgca aaagtcttgg gccccgcctt gggggcgagg ggggcgggag cgttcttggg
     3121 actcacgggt gctcgaccca gtcggcgatg aacaggttgg tctcgtgcgg caccgacgcc
     3181 cctcgattgg aggcccacac gagatgcttc ccgtccggcg agaacatggg aaacgcattg
     3241 aacgagtgat ccgtcgtgat cgtttccaga ttcgcgccgt cgagatccac cgcatacaag
     3301 tcgaagtgat cggactgagg gtccgtgtag ttcgacgaga agatgatccg cttcgacccg
     3361 ggatagaatg ccggcgcgaa ggacgccccg gcgagatggg tgatctgccg ctggttcgaa
     3421 ccgtccgcgt tcatcaccca caggtccatt ttcgtcggcc gcaccacccc ctgcgccagg
     3481 agcgtacggt actcggcgga gtcggcggag gtggacggat gataggcgcg atagacgatc
     3541 agcttcccgt cgagggagaa cgtaggaccg ccgtcgtagc cgagggtgtt ggtgagccgc
     3601 cggacgttgg ttccgtcggc gttcatcgtg tagatgtcga ggtctccgtc ccgctccgac
     3661 gtaaagacga tcgtcttccc atccggcgac agcgtgccct cggcgtcata gcccttgctg
     3721 ctggtcagct gcaccttgtg gctgccgtcc gcatcggacg cgtagatgtc gtagtcgtag
     3781 agggcccaga cgtacccgtg ggaataatcg ggacgtggag gacaggtgac ccctaccgcc
     3841 tcggtgctcg catagaacac ccgctggtcc cccgcgaaaa accagccgca ggtggttctc
     3901 ccctgcccgt ccgagacccg gtggagcccc gtgccgtcga cgttgatgat gaattcctga
     3961 tcgcagccgg cggtgtcggg gggaggcgtg tgctgaaaaa tcagccggga cccgtcgtgc
     4021 gagtagtacg cctcggcgtt ctggccgccg ttcgtgagac gccggagatt ccggagatgt
     4081 ggttcatcgg gacgggccgg gacggatgcc gcctggaaca tggaggcggc gagcaggaaa
     4141 gtgatcatgg gacgggcaag ctaggggcga gggcgaggcg gcgaaaggcc cgccccgcta
     4201 gatttctgga gagcgatgac cggcagtcct cccaaccccc ctcgcaagtc ccgcgtgccg
     4261 cttctggacg cgctgggaca actgtgcacc gacggcaagt ccacggccga gtatctctgg
     4321 caggttccgg acgacacgca ggcgcggctg aaactggtcg gcctgctcga ccagatcgcg
     4381 gtggaaagcg cgaagcaggg gcggaaggag atggggcgga tctgcgccga cctcgttccc
     4441 gcggccaagg cgagcccgag cccccagcag gtcgagctcc tggtggacgg tttcgaccgg
     4501 ctgttgaagc tttggcagtc tgcaaagtct ggcctcctct agaccttcac ggagattgga
     4561 tgacggacgt cgccgccggt cctcacaccg atttggatct gctctgcatc aacacggtgc
     4621 ggaccctcgc gatggatgcg gtgcaacagg ctgattccgg ccaccccggc acgccgatgg
     4681 ccctggcg
//
LOCUS       DASUBP010000240        24409 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_239841, whole genome shotgun sequence.
ACCESSION   DASUBP010000240 DASUBP010000000
VERSION     DASUBP010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 24409)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24409)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24409
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_239841"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /gene="rpsT"
                     /locus_tag="VFU75_09020"
     CDS             complement(<1..239)
                     /gene="rpsT"
                     /locus_tag="VFU75_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEU5467020.1"
                     /translation="MPKIKSAKKALRQARARTLVNRAQTSELRTALKRVRAAADKAAA
                     TTAYGRAVQLLDRAARKGLIHKNNAARHKSRLAALV"
     gene            351..1034
                     /locus_tag="VFU75_09025"
     CDS             351..1034
                     /locus_tag="VFU75_09025"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEU5467021.1"
                     /translation="MGHAISLDILLWAPVLLFSMVAHEYAHAAAAYRQGDDTAYALGR
                     LTLNPLKHIDPFMTVLLPILLWVASHGTWTFGGAKPVPVDPRKYREYRKGDIIVSLAG
                     IATNLAIFAVCVPLYVAAGLAGSAAPGVARVIQEMLVWGMYLNLVLAFFNLIPIPPLD
                     GSHVLYHALPPAARLRYRQIASFGFLPIMAVSYFLPGVMNVLLWPADQLMVGAGLIIK
                     GFTIKGGVL"
     gene            1055..1771
                     /locus_tag="VFU75_09030"
     CDS             1055..1771
                     /locus_tag="VFU75_09030"
                     /inference="COORDINATES: protein motif:HMM:NF014654.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HEU5467022.1"
                     /translation="MTGQPGFVIELEQFSGPLDLLLHLIREDEVDITDLPISKIADQF
                     LSVISELGLNQAADYLEMAARLLRIKAQMLLPRRLGDDEWEDPRSELVRRLLEYQQIR
                     ELVDWLQRAAATRAEQYGRGYEAPAPDAPPAPLEIELNDLLAAAERVISLIPEPMIHR
                     VVPRPLDVEGATRKIHALLESRGEFGWFDIVGETPTIVDVLSAFIALLELAKRQSVTL
                     EQPSPFAPMVIRRESPREAA"
     gene            1746..2294
                     /gene="scpB"
                     /locus_tag="VFU75_09035"
     CDS             1746..2294
                     /gene="scpB"
                     /locus_tag="VFU75_09035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEU5467023.1"
                     /translation="MSPLAKLLEAALFASSRPLTVEELATLDVESGEAAVRAALEEIR
                     AEFDGDRHGVSLTEIAGGWQFLTRREHAESIDRAAFALRPRRLSPAALETLAIIAYRQ
                     PVGRIEVDEIRGVDSGAVIDKLVERGLIEVASRGEGLGRPLLYGTTPQFLELLGLVDL
                     EELPRLEELSVALRPPAAAEPE"
     gene            2291..3016
                     /locus_tag="VFU75_09040"
     CDS             2291..3016
                     /locus_tag="VFU75_09040"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEU5467024.1"
                     /translation="MTEMRLQRALARAGVTSRRKAEDLIRAGRVRVDGVVAEIGSKVD
                     PGRQRVTVDGKPLAAPSVAWFALNKPIGYVVSRGDPEGRRTIFELLPKVPGLTYVGRL
                     DVMTSGLLICTTDGDAAHRMTHPKFAVERTYWLRVHGRPASEVRQALSGRPVIEGRPV
                     VIRAVRVRPAHRGKGSIEVELTLAEGRHRIVRRLAEELGLKVEWLHRVSYGPVRLGKL
                     PEGQWRALTPREIEAIGRIHAPR"
     gene            3003..4649
                     /locus_tag="VFU75_09045"
     CDS             3003..4649
                     /locus_tag="VFU75_09045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467025.1"
                     /translation="MHLADKTVMLLGGSGLVGHAVARRVLAKGPRRLILVALFEDEVK
                     SAAAALASHRGKVEIAVEWGNVFLPASVARHDRGDILADAKQRALVIQDQFGDLTDEV
                     LERNLLYQLLLKYRPDAVVDSINTATAFAYQDIFQSARDLLTASAAGTLDRALVEQHI
                     LTLTMPQLIRHVQIITEAMKRAGTSAYVKVGTSGTGGMGFNIPYTHSEERPSRTLLTK
                     SAVAGAHSLLLFLLGRTPEAPAVVEIKPTATIAWREIAFGPIRRKGKPLPLVDCPTPL
                     AVGEAFRDGAKPWKDQGKPVQGVFIDVGENGLFAKDEFATVTALGQMEFITPEEVADY
                     VIQELEGRPTGRDVIAALDGATAGPTYRAGLLRSAALGRLEELEKESATRSVGYEMLG
                     PPRLTKMLYEAFLLSRLRPSVRALAAADGTGLAREAQALIEKDAGLRAHILSVGIPIL
                     CADGAHVYRGSLVVVPPSASGDPLAAAPRGWVDLRPGYFGVWVTRAQAMVRAADLRTG
                     TKDSGSDVDWTAIGADDPVDPPRLATWVFSQEDKGERIKR"
     gene            4700..5689
                     /locus_tag="VFU75_09050"
     CDS             4700..5689
                     /locus_tag="VFU75_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU5467026.1"
                     /translation="MATTTGRTSKAVAADAALATALTREVSKRVVGQEVMVERLLIGL
                     LTGGHILLEGVPGLAKTLSVRTVAECLRISFSRIQFTPDLLPADVIGTMIYDQKTQEF
                     YPKKGPLFANLILADEINRAPAKVQSALLEAMQEKQVTIGGETFYLGEPFLVLATQNP
                     IEHEGTYALPEAQLDRFMLKVRVGYPTRDAEKEVVGRMASGRPIEVERIASGEDVLAA
                     RSAIAELYMDQKVVDYIVDVVRGTREPQSVGLPELKPLIAFGASPRASIYLAQAARAH
                     AYLRGRSYVVPEDVKAMAYDVLRHRVLLTFEAEAEDLDSDRVVGRILEAVGVP"
     gene            5686..6561
                     /locus_tag="VFU75_09055"
     CDS             5686..6561
                     /locus_tag="VFU75_09055"
                     /inference="COORDINATES: protein motif:HMM:NF017569.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEU5467027.1"
                     /translation="MTPEVLRQVKGIELRTRSLVNSLFTGEYRSVFRGQGIEFAEVRA
                     YQQGDDFRAIDWNVSARMGTPFVKEFMEERELTLLLIVDHSGSLHFGRPASKAGMAVE
                     IGAVLALAAARHSDRVGALIFSDRVEHVIRPAKGRGHALRVIRDLLAFAPRGRGTNLG
                     EALQYATKLLRHRAVVVVISDFRAEGWDEPLARLANKNDVVAITIDDPRETNLPDAGW
                     VELEDAETGRRVLVDTGHEGTRGKVRLAAERALQERQKKLRRAGVDVIALQTHVPYAQ
                     PLRRAFAERARRLKR"
     gene            6564..7388
                     /locus_tag="VFU75_09060"
     CDS             6564..7388
                     /locus_tag="VFU75_09060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467028.1"
                     /translation="MSPLLFVALMMRVAPADSQWMVAPPSPTVGDTIWLFRVVSHSPD
                     WRVRAGAFDNQGDIVSLGDPQVNFGSTADTVRYAVVAWTAGPHRFALPSLWLMGGAGR
                     SDSLPGGTGTVTVRSVIPADSARPSPKALLPPLDPQRVSLWPPFVAGALGVIALLLGV
                     RVRRRNTGAAAPPADFVTGGGAPDERWLAAGEPKAVAARAARVLRQALARVVPDAGES
                     LPVREALEAAEGKMPGTTFRRVRELLLALDQVAYAVAHGTDVARVAADARTLAREL"
     gene            7392..8375
                     /locus_tag="VFU75_09065"
     CDS             7392..8375
                     /locus_tag="VFU75_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU5467029.1"
                     /translation="MSFARPVLLLLLLAIPAWWWWRRRRLVRFGGVRMSDIGAAAGVG
                     GRRWPARLPLILRSLGVGAWIFAAAGPHVGLGRSEVKSEGISIVLAVDVSSSMLSQDF
                     APADRIDVAKQTAIDFVRHRPDDRIGLVAFAAQALSLVPITTDYGVLEQALGDLHVGT
                     VSEDGTAIGTAIGTAASRLRRAPGKSKVVVLLTDGVNNRGLVDPRTAAQAAAAFGVRI
                     YTIGVGTEGEAPVPTGQGLEGMRYQTMPVELDERLLGDVATMTGGRYFRATDSQSLRE
                     IFGEIDRLEKTPVRQVVSRRFDESYRTPLAIGLLVLALEIALSATLTVRVP"
     gene            8375..10021
                     /locus_tag="VFU75_09070"
     CDS             8375..10021
                     /locus_tag="VFU75_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEU5467030.1"
                     /translation="MTFDAPVVLLLAPLIAGAAWFGAAWARRVRLSRAAQWSVDTVVR
                     AQAAGRFAPTSLALATFCGTVALAGPRWGTERVTTQARGLDLIIAVDISRSMLAEDAG
                     GSRLAKAVREARRLVQDQPGDRFGLVAFAGAAYTLSPLTVDGSAISLYLESLDPDMAS
                     AGGTAVAPALQTSGALLDAAPDLSDRVLVVFTDGEAHDTVTDIAAQSERLAKDGVHVI
                     FVAEGEATPARIPVRDDHGALLGWQRDADSTVIGTRRRDDELTAFADAAHGTVVAADL
                     PDQAGAIRSLLAGYKRTGGTESRADTGRLQAWIPLLAGALILLGQAATRRAASLICLL
                     LLAGGIRGASAQRLSLRPRTEAAKAWDTRDTLGARAAYQAELNGRRTVDTAWYNAGTA
                     ALAAGDWESAQQGLSRAASSLDPEVRFRALYNLGVLDFRLARVDSANRDAHLGDAEKA
                     YREALLIKPHHAAAKWNLELATRRRGGSGGANNPSPQGGGGSNSQNPTPNGAGRGQDR
                     ALSTGQADEILRSIGQEELRARRDKTGRVKRSGEPDLKDW"
     gene            10021..12357
                     /locus_tag="VFU75_09075"
     CDS             10021..12357
                     /locus_tag="VFU75_09075"
                     /inference="COORDINATES: protein motif:HMM:NF024973.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HEU5467031.1"
                     /translation="MLLLLLALQAGGTRPTLTASVDHAHVRVGDELTLSIQAKSNSTD
                     PLTLLLPGLSGFTMVGSREATQVSFGSTGPGERVTLRELSLRAERPGRLVIGGIAVTQ
                     GSWRAKTAPIVVTVDSGVLATSVSAITRALLASAPPPSKTDQVSLTLVASRDSAWPGS
                     QLDVVAAAWFPRTVRNQLTRDPLLTLPTAVGAWGYPPDGPAGVATSRQVHGTWMDLYV
                     VHQTLFPLASGRLALPPATLEYALPVTASILTREERYELSSDSLVIAVPPLPVEGRPY
                     DDRGVVGHGLSLTVTTDSAPARVGEPMQLTATVAGTGNVTLWPAPVIAWPASLHAYPG
                     EEDTQIESRDGTVAGTKTIRYLVVPDSSGNVLLPEIRYPYFDPGTRSYAVPTVPPRVI
                     SVGVGMGLAAARQNLPLLPSREPAWAPFFTEAAWPWGWLAIALVPPVLGLLGVTIRRA
                     RSAPRPPAPARTWGTRLGRLERDFQDVLVTYVSDAEGRDGDALATALRAAGLEHAVAG
                     HVVRLRDRLRAARYGPQGAADQVELAEELTQVLKVLGGEPAGGGGRRHVRVLATLALV
                     FLAPQARGQAPSPETLYRAGAVRAAADAFAARVALHPDDAGAWYNLGAASYRQGADGR
                     AVAAWAEAARLAPRDGTIRKARAILAPPDLVTEDLLSVGPLTRAEWAVLAGLLWVAWW
                     GGVLLRRPRLKLSLGLALVVVLGMGAYEWWRLSRPVVVVVEGPAPVRSAPFGEASAAS
                     SLAAGAAGLVVKTYAGWVEVERPDGVRGWILQSEVMRL"
     gene            12354..14042
                     /gene="mutL"
                     /locus_tag="VFU75_09080"
     CDS             12354..14042
                     /gene="mutL"
                     /locus_tag="VFU75_09080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEU5467032.1"
                     /translation="MSGRIAVLPDRVADQIAAGEVVERPASVAKELVENALDAGAKHV
                     RVEIEDGGRTLIRISDDGHGMGRSDAKLALSRHATSKIRDASDLIGVLTFGFRGEALP
                     AIASVSRFELETVEAGDGQSGTRLSVVGGKLEGEEDIVRRPGTTVTARGLFFNTPARR
                     KFLRAPASETRAVSEVLTLLALARPDVAFSLSSDGRQLFDVPQATRPIDRIHALWGRD
                     LADSLLPVALRRGTVEVTGFVQRPSQAKPAGRKAHVFLRGRPIKDPFLIRAAEAGYRS
                     TIHPGDRPTILLFLDLPGDAVDVNVHPAKLEVRFRDRYAIEKIVEEAVREALAPLAAA
                     APLGDGPTGTAWGGLDQDIPEGQPITLFPAEVVSPEERRPVPALLQVFDTYILFQTDH
                     AVAIVDQHSAHERVLYEAVMRQLTGDGMPAQRLLLPLTLELTAGELDAVEQHKELLAR
                     VGFEVEMFSGRTLVAHTAPNPHPRFEAQRCLQELIADLAGGRFGGWQNRLERFAATFA
                     CRAAIKAGHRLDPEEMRELIYRLLTATLPAHDVHGRPTIVQLPADEVERRFGRS"
     gene            14029..14991
                     /gene="miaA"
                     /locus_tag="VFU75_09085"
     CDS             14029..14991
                     /gene="miaA"
                     /locus_tag="VFU75_09085"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEU5467033.1"
                     /translation="MGGADPARTMVIVGPTAAGKTALAVALAAHLPLTVISADARQVY
                     RGLDIGTAKPEPDVLRRVPHLGLDLIAVGERYSAGRFAADAAGWIGDVRRTGLTPVVV
                     GGTGFYIRALGDGLFQEPDLDPERREALRDWTDGLETGALVRWAGRLDPRFAGGGRQR
                     AARAIEVALLTGHPLSHWQEVARETGAIRPWYIQLTLPRDVLHRRIAERVDRMLERGL
                     VNEVRGVLATGVAPDAPGLDAVGYREVVAAERGELSWEAVRETIVIATRQYAKRQETW
                     FNNQVSGVRGQGSVAPVWKLDATLPPTELARLVFDRWSSLTPDP"
     gene            15011..16138
                     /gene="bshA"
                     /locus_tag="VFU75_09090"
     CDS             15011..16138
                     /gene="bshA"
                     /locus_tag="VFU75_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0071793 - bacillithiol biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HEU5467034.1"
                     /translation="MRIGITCYPTYGGSGAIATELGLELAKRGHEIHFVSYASPFRLP
                     SFAERVTFHEVTQAEYPLFETASPYALALAVKQHEVALREQLDIMHVHYAIPHAATAW
                     LAKQMLADQRDLKLVTTLHGTDITLVGKDPSYYTLTKFSIERSDRVTAVSKYLREETY
                     RAFGCQRCDVTVIPNFVSTTAYKAANESWFRRALGAGTMPILLHVSNFRPVKRVVDVV
                     QIFAGVRRVMPAMLVMVGDGPDRDAAQAEADGLGLKSDVRFLGKVDNVADVMRGADLF
                     LLPSEAESFGLAALEAMACGAPVIASNAGGLPELVVEGETGFLSAPGAVDDMTGNALK
                     VLQDSALAARMRAAAVERAQAFSVDKVVPQYERLYEDLLRG"
     gene            16131..16952
                     /locus_tag="VFU75_09095"
     CDS             16131..16952
                     /locus_tag="VFU75_09095"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="geranylgeranylglycerol-phosphate
                     geranylgeranyltransferase"
                     /protein_id="HEU5467035.1"
                     /translation="MVDLLRLVRAHNLVVAGLGVFAGGWIALGRVGAPGPLVWAVLSG
                     VGLGVAGNVLNDIWDEAGDRANARTDRPLASGRVRRGSADLLVFWGAFLGLGTAALVS
                     GTVFALAGGALVLMAAYSPCLKRRGVAGNLTVALVAGFPLMYGALSVGRGAAGLVPWV
                     LAGWLHFGREVAKDLVDVPGDRVLGRRTVPILSGEAVGRNVAKAILWSFIPASLALPA
                     LAGYGRWYFALAVFADVLVWSAGRALSGQRYPVAVRHAKLAMPLGVAALVLGRVV"
     gene            16949..17734
                     /locus_tag="VFU75_09100"
     CDS             16949..17734
                     /locus_tag="VFU75_09100"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEU5467036.1"
                     /translation="MTWWQGVILGLVQGLTEFLPVSSSGHLRVIEVLTGVKAPGVFVE
                     VSLHVATLFAVLVVYGASLWSIVRGTLSGDRASLRRAGLLVIATIPAAILGVLFHKQV
                     EEAATSLVLIGICFVVTGIALWSTKERHGMRPEPTVAGAVVIGLAQSVAALLRGISRS
                     GSTVTAALWVGLDPVDAAEFSFLLSIPIIAGAALVEGRHATVDIAAVGAGPLALSFAV
                     AFVAGIFSIRFLVAMLKRGRFYLFAPYCWAAGLFVIAYGLWHP"
     gene            17722..18474
                     /locus_tag="VFU75_09105"
     CDS             17722..18474
                     /locus_tag="VFU75_09105"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEU5467037.1"
                     /translation="MASLTLDGVSGGSLAKRWGVPQAGLFDSLGSTLDSIHELGTSGA
                     PSGTVVIADVQTAGRGRDGRTWRSPMGGLWLGVLLRPRTSDVGVYSIRVGLVLADAID
                     EVLGRPAARVKWPNDIVLADRKVAGILCEGRWQGETLHWLAVGIGCNVANELPPDVAG
                     LATRLADHRPGATRLDLCDRLVPALPRLGAAGLRLTEAELTAFGSRNWLAGRQIRAPL
                     AGKVIGLEGNGALLVETARGRQTVREGHVELA"
     gene            18491..19270
                     /locus_tag="VFU75_09110"
     CDS             18491..19270
                     /locus_tag="VFU75_09110"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009855.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HEU5467038.1"
                     /translation="MLLALDVGNTETTLGLFDGDQLAHHWRLTTTVQRTPDEWGAALT
                     SYLMQAGHSTGEVRAAIVASVVPLVSHALGEGIRIAIGQAPTFVDARSRLPITLDVDE
                     PLTVGADRIVNTLAASQVYKDDCIVVDFGTATTFDCITKDGRFIGGVIMPGVVTAANE
                     LVRKTAKLPAAELSAPPHVIGRRTEDCIRSGVLFGAAEAIDGLIRRVRAEWPEGRKPR
                     AIATGGLATLIAPLCKEIGGTDPDLTLTGLRLAAAVLGLKW"
     gene            19264..20193
                     /locus_tag="VFU75_09115"
     CDS             19264..20193
                     /locus_tag="VFU75_09115"
                     /inference="COORDINATES: protein motif:HMM:NF013226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HEU5467039.1"
                     /translation="MVTDAHATKTLNVWRRAGYGALPGDAFSYILHMRPAEWPIMAAH
                     TVLGYVLAVGWSGLRSGQQLGSALFALFIWVICLNGGTLAINSVYDKDEGDVGYLNSP
                     PPLPKHLLTFSWVLLVLGQVLAFRLPWGFRIAYAVCFVMSVAYSVPPLRFKAVAGLDW
                     VINMWGFGTLTPYAAWAATGRPLDLGHAVIFLSFCALFAGLYPLTQLYQLEEDKKRGD
                     RTLALMFGLEASLWLATGSVILAFGGLSWAADILRRSGTLIFFALAAWLAVLVPWIRA
                     RNVWTPQQHQRGMYWALGAWAVTDVAVLLVFAR"
     gene            20241..20858
                     /locus_tag="VFU75_09120"
     CDS             20241..20858
                     /locus_tag="VFU75_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467040.1"
                     /translation="MSATATPTMTDRTELPHVLISGAKLGVLTAVWVVLYLVIWRYVP
                     GTVGGVLDAVIVLLGGLSMALLPAEWTGARHTEGIAGAAGVGLFSTVVFSAIDIIILR
                     NVHAYPWTWDAIGGNSTWWYLPTWWQLGTFVSWMGAMLTAGRNSKHAASMTSLALPLV
                     VGAVVIGEIAHLMGFGFTSTYPVAIGLGFSVILTVLGVAAIARKA"
     gene            20855..21145
                     /locus_tag="VFU75_09125"
     CDS             20855..21145
                     /locus_tag="VFU75_09125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467041.1"
                     /translation="MTDGGVPRWLAHFLLVVGWLLTPLCAWGASYFGLWIGAKVGVRF
                     SGPGAMVGVALGIAFLSGFVMLALWVRMMRRFPHWLAHRMAPRPSQENRATE"
     gene            21132..22136
                     /locus_tag="VFU75_09130"
     CDS             21132..22136
                     /locus_tag="VFU75_09130"
                     /inference="COORDINATES: protein motif:HMM:NF013073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEU5467042.1"
                     /translation="MRRNDVGFLIVTLSLAATSLAAQGEIEFRYGQWYRAGHGATYEF
                     RSHSPLTGVISQTFGATVIVNDRLGRRQAFYGGGWEAHLFRGSYTFGPYGLLGVALGL
                     STDTAKQELAALWSVGGGLEWRPFDRIALGVEGRYRDEDRGPRGFWSGGGSPRKGFSL
                     SLGLAIGLGRHGGRMSAPSNPVLPPPASTVPSNAPVQPPTTISGNAAAADVVRTALDV
                     LGTPYQWGGTASNGFDCSGLIQYAYGQHGVRLPRISRDQARAGAEVTPVVEALQPGDI
                     LLFSASPGAGVTHVGMYVGEGKFIHSASSGVKISRLDRSDSDGAYWIPRWVGARRILP
                     "
     gene            22133..22516
                     /locus_tag="VFU75_09135"
     CDS             22133..22516
                     /locus_tag="VFU75_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467043.1"
                     /translation="MSGRWAVALGLAFACAGNPGPAGPPSPLRGYDIVIRGRDSLSHA
                     LQGAFARAGYTVRDDVRGGGHMAAALVWWKYVDQEGRGTLEAQVADTRRGHVLAVATI
                     PADTLPGDWQGRAELLVKALLNPSP"
     gene            22624..22935
                     /locus_tag="VFU75_09140"
     CDS             22624..22935
                     /locus_tag="VFU75_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEU5467044.1"
                     /translation="MATGSKGGKHKLLPLGDRVAIKPAEETETMKGGLYIPDTAKEKP
                     IQGDVVAVGPGRTEKGEKVPMELKVGDRVIYGKYSGTPVELDGEEVILIKESDVIAKL
                     G"
     gene            22950..>24409
                     /gene="groL"
                     /locus_tag="VFU75_09145"
     CDS             22950..>24409
                     /gene="groL"
                     /locus_tag="VFU75_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004211302.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEU5467045.1"
                     /translation="MAAKFLEFNVEARARLKRGVDQLADAVKVTLGPKGRNVVIEKKF
                     GNPTVTKDGVTVAKEIELEDEIENMGAQMVKEVATKTSDLAGDGTTTATVLAQAIYRE
                     GLKSVTAGVNPMALKRGIDKAVETIIEELKRISVPTKGRKEIAQVGTISANGDKEIGD
                     KIADAMDKVGKDGVITVEEAKGLETTLETVDGMQFDRGYLSPYFVTDPEKMEAVLEDA
                     YVLIHDKKISSMKDLLPALEKVAQSGKPLLIIAEDVEGEALATLVVNKLRGTLKVAAV
                     KAPGFGDRRKEMLIDIEKVTGGKVVAEEKGMKLENTVLGDLGQAKRIVVDKDNTTIVG
                     GRGKQADIQGRIGEIRAAIDKSTSDYDKEKLQERLAKLAGGVAVINVGAATETEMKEK
                     KARVEDALHATRAAVEEGIVPGGGSALLVCQKSLDKLKGGDDDEKIGVDIVRRAIEEP
                     IRMIAQNAGAEASIVVGKVKESKDKNFGYNASTNEY"
BASE COUNT         3586 a         8036 c         8718 g         4069 t
ORIGIN      
        1 accaacgctg ccagccggga cttgtgccgg gcggcattgt tcttgtggat cagccccttc
       61 cgagcggccc gatcgagcag ctggacggcg cggccatacg cggtggtcgc ggcggccttg
      121 tcggcggcgg cgcggacgcg cttcagggcg gtccggagct ccgaggtctg agcacggttg
      181 acgagggtgc gggcgcgggc ctggcgcagc gccttcttcg cggacttgat tttcggcact
      241 tggtctcctg ttcgaggcgc ggaaaataga cggtaagtta tgggggatca aggccctacg
      301 acgttgaccg ggaggggggc gatttcctat cttacagcgt cgcaaacgac ttggggcacg
      361 ctatctcgct cgacatcctg ctctgggccc cggtgttgct gttctccatg gtggcgcatg
      421 aatatgccca cgccgcggcc gcctaccgtc aaggtgatga taccgcctac gctctggggc
      481 ggctgacgtt gaaccccctg aaacacatcg acccattcat gaccgtgctc ctgccgatcc
      541 tcttgtgggt ggcgtcgcac gggacttgga ccttcggggg cgccaagccg gtcccggtcg
      601 acccgaggaa gtaccgtgaa taccggaagg gcgacatcat cgtgtccctc gccggaatcg
      661 ccacgaacct ggcgatcttc gctgtctgtg tgcccctgta cgtcgcggcg ggtttggccg
      721 gatcggccgc accgggagtg gcgcgcgtga tccaggaaat gctcgtctgg gggatgtatc
      781 tcaatctggt tctcgcattc ttcaacctga taccgattcc tccgctggac ggaagccatg
      841 tactgtacca tgcgctgcca cctgccgcga ggttgagata ccggcagatc gccagtttcg
      901 ggttcctgcc catcatggcg gtcagctact ttctgccagg ggtgatgaac gtgctgctct
      961 ggcccgccga ccagctgatg gtgggagccg gattgatcat caaagggttc acgatcaagg
     1021 gcggggtact atgagtgcac cgaccaatcc tgtcatgacc ggacaaccgg gtttcgtcat
     1081 cgagctggag cagttctccg gccccctgga tcttctgctg cacctgatcc gggaagatga
     1141 agtcgacatc accgacctgc ccatctcgaa gatcgcggac caattcctgt ccgtcatcag
     1201 cgagctcggc ctgaatcagg cggcggacta tctcgaaatg gccgcccgcc tgctgcgcat
     1261 caaggcccag atgctgctcc cgcgccgcct cggcgacgac gagtgggagg acccgcgctc
     1321 ggagctggtg cgacgtctgc tcgagtacca gcagatccgt gagctcgtgg actggttgca
     1381 gcgagccgcc gcgacgcgcg ccgagcagta tggccgcgga tacgaagccc cggcgcccga
     1441 tgcgcccccc gcgccactgg agatcgagct gaacgacctc ctggcggcgg cggagcgggt
     1501 gatctcgctc attccggagc cgatgattca ccgcgtcgtg ccgcgcccgc tggacgtgga
     1561 gggcgcgacc cggaagatcc acgcgctgct cgagtctcgc ggcgagttcg gctggttcga
     1621 catcgtgggc gagacgccca ccatcgtcga tgtgctgtcc gcgttcatcg cgctcctcga
     1681 gctggccaag cggcagtcgg tcacgctcga gcagccttct cccttcgcac ccatggtgat
     1741 ccgacgtgag tcccctcgcg aagctgcttg aggccgcgct cttcgcgtcg tcccggccgc
     1801 tgacggtcga ggagctcgcg acgctcgacg tcgagtcggg ggaggcggcg gtgcgtgcgg
     1861 ccctcgagga gattcgcgcc gaattcgacg gcgaccgcca tggcgtgagc ctgacggaaa
     1921 tcgcgggcgg ctggcagttc ctcacccgcc gcgagcacgc tgagtcgatc gatcgggcgg
     1981 ccttcgcgct gcgtccgcgg cgtctgtcgc ccgcggcact cgagacgctg gccatcatcg
     2041 cctaccggca gccggtggga cggatcgaag tggacgagat ccgcggcgtg gattcgggag
     2101 ccgtgatcga caagctggtc gagcgcggcc tgatcgaagt cgcgtcccgc ggcgagggac
     2161 tgggccgccc gctgctctac ggcaccacgc cgcagttcct cgagctcctt gggttggtag
     2221 acctggagga gctgccgcgg ctcgaagagc tgtcggtcgc cctccgcccg cccgccgcgg
     2281 ccgaacccga atgacggaga tgcgcctgca gcgcgctctg gcgcgcgcag gcgtgacgtc
     2341 ccggcgcaaa gccgaggact tgatccgcgc cggacgggtg cgggtggacg gcgtcgtcgc
     2401 cgagatcggc tccaaggtcg accccggccg gcagcgcgtc accgtcgacg ggaagcccct
     2461 cgccgcgccc tcggtcgcct ggttcgccct caacaagccg atcgggtacg tcgtgagccg
     2521 cggagatccc gagggacgga ggaccatttt cgaactcctc ccgaaagtcc ccgggctcac
     2581 ctacgttggg cggctggacg tgatgacgtc ggggctcctg atctgcacca ccgacggcga
     2641 cgcggcccac cggatgacgc atccgaagtt cgccgtggag cggacctact ggctgcgggt
     2701 gcacggccgg ccggcgtccg aggtacggca ggcgctctca ggccgccccg tgatcgaagg
     2761 gcgaccggtc gtgattcgcg ccgtccgcgt gcgcccggcc catcgcggca aggggagcat
     2821 cgaggtcgag ctgacactgg cggagggacg gcatcggatc gttcgccgtc tcgcggaaga
     2881 gctgggactc aaagtcgagt ggctgcaccg cgtgtcgtac gggccggtgc ggctgggcaa
     2941 acttccggaa gggcagtggc gcgcgctcac cccacgcgag atcgaggcca tcggacggat
     3001 ccatgcacct cgctgacaag accgtcatgc tgctcggagg atcggggctc gtggggcacg
     3061 cggtcgcccg gcgggtgctg gcgaagggcc cccggcggct gatcctggtg gcgctgttcg
     3121 aggacgaggt aaaaagcgcg gcggcggcgc tcgcctccca tcgcggcaag gtggagatcg
     3181 ccgtggagtg ggggaacgtg ttcctgccgg cgtccgtcgc tcggcacgac cgcggagaca
     3241 ttctggcgga cgcgaagcag cgtgcgctcg tgatccagga tcaattcggg gacctgacgg
     3301 acgaagtcct ggaacgcaat ctcctctatc agctgctgct caagtaccgc cccgacgccg
     3361 tggtcgacag catcaatacc gccaccgcct tcgcctatca ggacattttc cagagcgccc
     3421 gcgacctgct cacggcgtcc gcggcgggca cgctcgaccg ggcgctggtg gagcagcaca
     3481 tcctcacgct caccatgccc cagctgatcc gccacgtgca aatcatcacc gaagcgatga
     3541 aacgggcggg gacctctgcg tatgtaaagg tgggcacgag cggtaccggg ggcatgggat
     3601 tcaacatccc gtacacgcat tccgaagagc gcccgagccg gacgctcctg accaagtccg
     3661 cggtcgccgg ggcccactcg ctcctgctct tcctgctggg gcgcaccccg gaagctcccg
     3721 cggtggtgga gatcaagccc accgccacca tcgcgtggcg cgagatcgcg ttcgggccga
     3781 ttcgccgtaa gggaaagccg ctgcccctgg tggactgccc gacgccgctc gccgtagggg
     3841 aggccttccg cgacggggcg aagccctgga aggatcaagg aaagccggtg cagggcgtat
     3901 tcatcgacgt cggggagaac ggcctgttcg ccaaggacga attcgccacg gtgaccgcct
     3961 tggggcagat ggagttcatc acgcccgaag aggtcgccga ctacgtcatt caggagctcg
     4021 aaggacgtcc gaccggaaga gatgtgatcg ctgccctgga cggcgcgacc gccggaccca
     4081 catatcgcgc cgggctgctg cggtcggccg cgctgggtcg gttggaggag ctggaaaagg
     4141 agtcggcgac gagatcggtg ggctacgaaa tgctgggccc gccccggctg accaagatgc
     4201 tctacgaggc gttcctgctc tccaggctcc ggccctcggt gcgcgccctg gcggcggcgg
     4261 atgggacggg cctggcgcgg gaggcgcagg cgctcatcga gaaggatgcc gggctgcgcg
     4321 cccatatcct ctcggtcggg atcccgatcc tgtgcgctga cggagcgcac gtgtaccggg
     4381 gaagtctcgt ggtcgtaccg ccttccgcga gtggcgaccc ccttgcggcg gctccgcgag
     4441 ggtgggtaga cctccggccg gggtacttcg gggtgtgggt tacgcgggca caggcgatgg
     4501 tgcgcgcggc cgacctcagg accgggacga aggactcggg cagcgacgtc gactggacgg
     4561 cgatcggcgc ggacgatccg gtggatccgc cgcgcctcgc cacctgggtc ttcagccagg
     4621 aagacaaggg tgagcggatc aagcggtagg aggcggcagg ggcagcaggg gacggacgat
     4681 acttgaggga ggcgttggag tggcaacgac gactggacgg acctcgaaag ccgtggcggc
     4741 tgatgcggcg ctcgcgaccg ccctgacccg cgaagtttcg aagcgcgtcg tggggcagga
     4801 ggtgatggtc gagcgcttgc tcataggatt gctgacgggt ggtcacattc tgctggaagg
     4861 tgtcccgggg ctcgcgaaga cgctctcggt gcgcaccgtg gccgagtgtc tgcggatcag
     4921 tttctcacgg atccaattca cgcccgacct gcttcccgcc gacgtcatcg gcaccatgat
     4981 ctacgatcag aagacgcagg agttttatcc caagaagggc ccgctcttcg cgaacctgat
     5041 cctcgcggac gaaatcaacc gcgcaccggc caaggtgcag tcggcgctcc tcgaggccat
     5101 gcaggagaag caggtgacca tcggcggcga gaccttctac ctcggcgagc ccttcctggt
     5161 gctcgcgacg cagaacccga tcgagcacga aggcacctac gccttgcccg aagcccagct
     5221 cgaccggttc atgctcaagg tgcgcgtcgg gtaccccacg cgggacgccg agaaggaagt
     5281 cgtggggcgg atggcgtcgg ggcgtccgat cgaagtcgag cggatcgcct caggggaaga
     5341 cgtgctggcc gcacgcagtg ccatcgccga gctgtacatg gaccagaagg tggtcgacta
     5401 catcgtggat gtcgtgcgcg gcacccgcga gccgcagtcg gtcggactgc ccgagctcaa
     5461 gccgctgatc gccttcggcg cgagcccgcg cgcgtccatc tatctggcgc aggcggcgcg
     5521 ggcgcacgcg tatctccgcg gccgttccta cgtcgtgccg gaagacgtca aagcgatggc
     5581 gtacgacgtc ctgcgccacc gcgtcctgct caccttcgag gccgaggccg aagacctgga
     5641 ttcggaccgg gtcgtcggcc ggatcctgga ggcggtcggc gtgccgtgac ccccgaggtt
     5701 ctgcggcaag tcaaggggat cgagctccgc acccggtccc tggtcaattc cctgttcacg
     5761 ggcgagtacc ggtcggtgtt ccgcgggcag ggcatcgagt tcgccgaagt gcgcgcctac
     5821 cagcagggtg acgacttccg cgccatcgac tggaacgtct ccgcccgcat ggggaccccg
     5881 ttcgtcaagg agttcatgga ggagcgcgag ctcacgctgc tcctcatcgt ggaccattcg
     5941 ggctcgctcc actttggacg cccggcgtcc aaggccggca tggcggtcga gatcggcgcc
     6001 gtcctggccc tggcggcggc gcgtcactcg gaccgcgtcg gggcgctgat cttttccgat
     6061 cgcgtggagc acgtcatccg tcccgccaag ggacgggggc acgccctgcg cgtcatccgc
     6121 gacctcctcg ccttcgcgcc ccggggccgc ggaacgaacc tcggcgaagc gctgcaatac
     6181 gcgaccaagc tcctgcggca ccgggccgtg gtggtcgtga tttccgattt tcgcgccgaa
     6241 ggctgggacg agccgctggc gcgcctcgcc aacaagaacg acgtcgtcgc catcaccatc
     6301 gatgacccgc gcgagaccaa cttgcccgac gccgggtggg tggagctcga ggacgcggag
     6361 accggacgcc gcgtcctggt ggatacggga catgagggaa cccgcgggaa ggtccggctc
     6421 gccgcggagc gggcccttca ggaacggcag aagaagctcc gccgcgccgg cgtcgacgtc
     6481 attgcactgc aaacgcacgt cccctacgca cagcccctcc gccgcgcctt cgcggagcgg
     6541 gcccggcggc tcaagcgctg accgtgagtc cgctcctctt cgtggcgctg atgatgcggg
     6601 tggcacccgc ggacagtcag tggatggttg cgccaccgtc gcccaccgtc ggagacacca
     6661 tctggctctt ccgcgtcgtc tcgcactcgc ccgactggcg cgtgcgggcc ggggcgttcg
     6721 acaaccaggg cgacatcgtc tccctcggag acccccaggt gaatttcgga agcaccgccg
     6781 acaccgtccg gtacgccgtg gtggcgtgga cggcgggacc gcatcgtttc gcgctgcctt
     6841 cactgtggct gatgggtggc gcgggtcgct cggactccct ccccggggga accgggaccg
     6901 tcacggtgcg cagcgtaatt cctgcggaca gcgcccgccc gtcaccgaaa gcgctcctgc
     6961 cgcccctgga tccacaacgg gtttccctct ggcccccgtt cgtcgccgga gccctgggcg
     7021 tcatcgcgct gctgcttggg gtgcgcgtgc ggcggcggaa caccggcgcg gccgcgccgc
     7081 ccgccgactt cgtcaccggt ggcggggctc ccgacgaacg ctggctcgcg gcgggggagc
     7141 cgaaggccgt ggcggcccgc gccgcccggg tgctccgcca ggcgctcgcg cgcgtagtcc
     7201 ccgacgcggg ggagtccctg ccggtgcgcg aggcgctcga ggcggctgag ggcaagatgc
     7261 ccggcacgac cttccggcgc gtgcgggagt tgctgctcgc cctcgatcag gtggcgtacg
     7321 ccgtggcgca cgggaccgac gtcgcgcggg tggcggcgga cgcccgcacg ctcgcgcggg
     7381 agctttgacc cgtgagcttc gcgcgcccag tccttctctt gctgttgctc gccatcccgg
     7441 cctggtggtg gtggcgccgg cgccggctgg tgcggttcgg cggcgtccga atgagcgaca
     7501 tcggagcggc ggcgggtgtg gggggccggc gctggcccgc ccgcctgccg ctgatcctcc
     7561 gctccctcgg cgtcggcgcg tggattttcg ccgcggcggg gccgcacgtc ggcctgggcc
     7621 gctcggaagt gaagagtgaa gggatctcga tcgtgctcgc ggtggacgtg tcgagcagca
     7681 tgctctcgca ggatttcgcg cccgccgacc gcatcgacgt cgcgaagcag accgccatcg
     7741 acttcgtccg gcaccggccc gacgaccgca tcggtctcgt ggcgttcgcc gcccaggccc
     7801 tgtccctggt gcccatcacg accgattacg gtgtcctcga acaggcgctc ggcgatctgc
     7861 acgtcgggac cgtgagcgag gacggcaccg ccatcggtac ggcgatcggg acggcggcga
     7921 gccgcctgcg ccgggccccc gggaaatcca aggtcgtggt cctgctcacc gacggcgtga
     7981 acaaccgggg actcgtggac ccccggaccg cggctcaggc ggccgcggca ttcggcgtcc
     8041 ggatctacac gatcggcgtg gggacggaag gagaagcgcc ggtgcccacg gggcagggac
     8101 tcgagggcat gcgctaccag accatgcccg tcgagctgga cgaacgcctc ctcggcgacg
     8161 tggcgacgat gacgggcgga cgctacttcc gcgcgaccga ctcccaatcg ctccgcgaga
     8221 ttttcggcga gatcgaccgg ctcgaaaaga caccggtgcg ccaggtggtg agccgccgct
     8281 tcgatgaatc gtaccggacg ccgctcgcca tcggcctcct ggtcctcgcg ctcgagatcg
     8341 ccctgtcggc caccctcacc gtgcgggtgc cctgatgacc ttcgacgcgc cggtcgtact
     8401 cctcctcgcc ccgctgattg ccggcgccgc gtggtttgga gcggcgtggg cccggcgggt
     8461 ccggctgtcc cgggcggcgc agtggtccgt cgacacggtg gtgcgggccc aggccgcggg
     8521 ccggttcgct cccacgtcgc tggcgctggc gacgttctgt ggaacggtgg cgctcgccgg
     8581 accgcgctgg ggcacggagc gcgtcaccac gcaggcccgg ggactcgacc tgatcatcgc
     8641 ggtcgacatt tcccgctcga tgctcgcgga agacgcggga ggctcccggc tggcgaaagc
     8701 cgtacgcgag gcgcgccggc tggtgcagga tcagcccggc gaccgcttcg gacttgtggc
     8761 attcgcgggc gcggcctata cgctgtcgcc gctgaccgtc gatgggtccg ccatcagcct
     8821 ctatctcgaa tcgctcgatc ccgacatggc ctccgcgggg ggaacggcgg tggctcccgc
     8881 cctgcagacg tcgggagcgc tcctcgatgc ggctcccgat ctctccgacc gcgtgttggt
     8941 ggtgttcacc gacggcgagg cgcacgacac ggtcacggac atcgcggcgc agagtgagcg
     9001 cctggcgaag gacggcgtcc acgtaatctt cgtcgccgag ggggaagcga cgccggcccg
     9061 gatccccgtc cgggatgatc atggcgctct gctggggtgg cagcgggacg cggattcgac
     9121 cgtgattgga acgcggcgcc gggatgatga gctgacggcc ttcgcggacg ccgcgcacgg
     9181 cacggtggtg gccgcggacc tccccgatca ggccggcgcg attcggtccc ttctggccgg
     9241 atacaaacgc accgggggta cggagtcgcg ggccgacacg ggtcgcctcc aggcatggat
     9301 tccgctcctc gccggtgcac tcatcctcct cgggcaggcc gcaacccgcc gggccgcctc
     9361 actcatctgc ctccttctcc tggccggcgg gattcgcggg gcatcggcgc agcggctgtc
     9421 gctccgtccg cggactgagg ccgcgaaggc gtgggacacg cgggacacgc tcggtgcccg
     9481 ggccgcctac caggcggagc tgaatggccg gcgcactgtc gacaccgcct ggtacaacgc
     9541 cggaaccgcc gccctggcgg ccggagactg ggaatccgcg cagcagggac tctcccgggc
     9601 cgcctcgtcc ctcgatcccg aagtccgctt ccgcgcgctc tacaacctgg gcgtgctcga
     9661 cttccggctc gccagggtcg acagcgcgaa ccgggatgca cacctcgggg acgcggagaa
     9721 ggcgtatcgc gaagcgctgt tgatcaagcc gcatcatgcc gcggcgaaat ggaatctcga
     9781 gctcgccacg cgccgccgcg gcgggagtgg cggcgcgaac aacccgtcac cccagggggg
     9841 cgggggttcg aacagccaga acccgacgcc caatggtgcc ggacggggtc aggaccgcgc
     9901 gctctcgacc ggacaggcgg atgagatctt gcgctccatc gggcaggaag agctgcgtgc
     9961 gcgccgggac aagacgggcc gggtgaagcg gtcgggagaa cccgacttga aggactggtg
    10021 atgttgctgc tcctcctggc gttgcaggct gggggaacgc gaccgaccct gacggcgtcg
    10081 gtcgatcatg cgcacgtccg ggtcggcgac gagctgacgc tttcgattca ggcgaaaagc
    10141 aattcgacgg atcctctcac cctgctgttg cccggccttt cgggcttcac catggtaggc
    10201 tcgcgtgagg cgacccaggt atcgttcggc agcacggggc ctggcgagcg agtgaccctg
    10261 cgcgaattgt cgctgcgagc cgagcgcccg gggcggctgg tgatcggcgg catcgcggtg
    10321 acgcagggct cgtggcgggc caagacggcg cccatcgtgg tgacggtgga ctccggcgta
    10381 ctcgccactt ccgtcagtgc catcacgcgc gcgctcctgg cgtcggcccc accaccatcg
    10441 aaaacggacc aggtctccct gaccctcgtc gcctcacgtg actccgcgtg gccggggtct
    10501 caactcgacg tggttgcggc ggcctggttc ccgcgcacgg tgcggaatca gctcacgcga
    10561 gatccgttgc tcaccttgcc gacggctgtg ggcgcgtggg gatatcctcc cgacggcccc
    10621 gccggcgtcg cgacgagcag gcaagtccac ggtacctgga tggatctgta cgtcgtccac
    10681 cagaccctct ttccgctggc gagcggacgc ctggcgctgc cgccggcgac gctcgagtac
    10741 gcgctcccgg tcaccgcgtc catcctcact cgtgaggagc ggtacgagct gtcgagtgac
    10801 tcgttggtca tcgcggttcc gcccctcccg gtggagggga gaccctatga cgaccgtggc
    10861 gtggtgggcc acggattgtc tctcaccgtg acgacggact ccgcgcccgc ccgggtgggg
    10921 gagccgatgc agctgacggc gaccgttgcg ggcaccggca atgtcacgct ctggcccgcg
    10981 ccggtgatcg cgtggccggc gtcgctgcac gcgtatcccg gtgaggagga cacccagatc
    11041 gaatcgcggg acggaacggt ggcgggcacc aagacgatcc ggtatctcgt ggtgccggat
    11101 tcgagcggaa acgtgctgct cccggaaatt cgctacccat atttcgatcc tggcacgcgc
    11161 tcatatgcgg tgccgacggt cccgcctcgc gtcatctcgg tgggtgtggg aatggggctg
    11221 gccgcggccc ggcagaactt gccgctgctc ccgtcgaggg aacccgcgtg ggctccgttc
    11281 ttcacggagg cggcgtggcc ttggggatgg ctcgcgatcg cgctggttcc gccagttctc
    11341 ggactcctcg gcgtcaccat ccggcgcgcc cgatcggccc cgcggcctcc cgcccccgcg
    11401 cgcacttggg gaacgcgcct gggcagactg gagcgggatt tccaggacgt cctcgtgacc
    11461 tacgtgtccg atgccgaagg acgtgacggc gacgcgctgg ccacggcgct tcgggcggcg
    11521 gggctggaac acgccgtggc ggggcacgtc gtccggctcc gcgaccggct gcgagcggcg
    11581 cgctacgggc cgcagggggc cgcggatcag gtggagctgg cggaagagct gacgcaggtt
    11641 ctcaaagtgc tgggcggcga gccggccggg ggcggggggc gtcgccacgt tcgtgtcctg
    11701 gcgaccctcg cgctggtctt cctggcccct caggcccgtg ggcaggcgcc cagtcccgag
    11761 accctctatc gggccggcgc cgtccgggcc gccgcggacg cgttcgccgc ccgcgtcgcc
    11821 cttcaccctg acgatgccgg ggcgtggtac aacctggggg ccgccagcta ccgccagggc
    11881 gcggatggca gggcggtggc ggcgtgggcc gaagccgcgc ggctcgcgcc cagagatggc
    11941 accattcgca aggcccgggc tattctggct ccccctgacc tcgtcaccga ggatctgctc
    12001 tcagtcggtc cgctcacgcg agcggagtgg gcagtgctcg ccgggctgct ctgggtggcc
    12061 tggtggggcg gcgttctgct gcgccgcccg cggttaaagt tgtctctggg cctcgccctg
    12121 gtggtggtcc tgggcatggg tgcgtacgag tggtggcgct tgtcccggcc cgtggtggtg
    12181 gtcgtggagg gaccagcccc cgtccgcagt gcgccgttcg gggaagccag tgctgcgtcg
    12241 tcgttggccg cgggagcggc tggattggtg gtgaagacct atgccggatg ggtcgaagtg
    12301 gagcgccccg acggcgtgcg cggatggatt cttcaatcag aggtgatgcg gctgtgagtg
    12361 gacggatcgc cgtccttccg gaccgggtgg ccgaccagat cgcggccggc gaggtcgtcg
    12421 agcgtccggc gtcggtcgcg aaggagctgg tcgaaaacgc gctcgatgcc ggggccaagc
    12481 acgtgcgggt ggagatcgag gacggcgggc gcacactgat ccgcatctcg gatgacggac
    12541 atgggatggg gcgctccgac gccaagctcg cgctctcccg acacgccacc agcaagatcc
    12601 gcgatgcctc cgacctgatc ggcgtcctca cgttcggttt tcggggcgag gcgctcccgg
    12661 ccatcgcctc cgtgtcgcgg ttcgagctgg agacggtcga agccggtgat ggccagtcgg
    12721 gcacccgtct gtcggtggtg ggcggaaagc tcgaagggga agaggatatc gtccgccggc
    12781 cgggaacgac ggtgacggcc cgcggcttgt tcttcaatac tccggcgcgc cggaagttcc
    12841 tgcgggcacc cgcatccgag acgcgggccg tgagcgaggt gctgaccctg ctcgccctcg
    12901 cgcgtcccga cgtggcgttc agcctttcga gtgacggacg ccagctcttc gacgtaccgc
    12961 aggccacccg tcccatcgac cgcattcacg cgctctgggg tcgcgacctg gcggactcgc
    13021 tgcttccggt ggcgcttcgc agggggacgg tcgaggtcac gggtttcgtg cagcgtccct
    13081 cgcaggcgaa gccggcgggg cgcaaggcgc acgtcttcct gcgcggccgc ccgatcaagg
    13141 atccgttcct cattcgcgcc gccgaggccg ggtaccggtc caccattcac cccggggacc
    13201 gccccaccat tcttctgttt ctcgacttgc cgggcgacgc cgtagacgtg aacgtgcacc
    13261 cggccaagct cgaggtgcgc ttccgcgacc ggtacgcgat cgagaagatc gtcgaagagg
    13321 cggtccgcga agcgctcgcg ccgctcgccg cggccgcgcc cctcggcgac ggtccgaccg
    13381 gaaccgcgtg ggggggcctg gaccaggaca tccccgaggg acagccgatc acgttgttcc
    13441 ccgcggaggt ggtgtcaccc gaagagcggc gcccggtgcc ggccctcctt caggtcttcg
    13501 acacctacat cctattccag accgaccatg cggtggccat cgtcgaccag cactcggcgc
    13561 acgaacgggt cctgtatgag gcggtgatgc ggcagcttac gggggacggc atgcccgcgc
    13621 agcggttgct gctcccgctc acgctcgagc tgaccgccgg cgagctcgac gcggtcgagc
    13681 agcacaagga gttgctggcc cgggtgggct tcgaggtcga gatgttctcc ggccgtacgc
    13741 tggtcgcgca caccgcgccc aacccccatc cgcgcttcga ggcccagcgc tgtctgcagg
    13801 agctcatcgc ggatctggca gggggccgct tcggcgggtg gcagaaccgg ctcgaacgat
    13861 tcgcggcgac cttcgcgtgt cgcgcggcga tcaaggccgg gcatcgactc gaccccgaag
    13921 aaatgcggga gctgatctac cgtctgctca cggccactct gccggcccac gatgtgcacg
    13981 ggcggcccac catcgtgcag cttcccgcgg acgaggtgga gcggcgcttt gggcggagct
    14041 gacccggcaa gaaccatggt gatcgtgggg cccacggcgg cggggaaaac cgcgctcgcc
    14101 gtggctctcg ccgcgcacct cccgctcacc gtgatctcgg ccgacgcgcg ccaggtgtac
    14161 cgcggtttgg atatcggcac cgccaagccg gagccggacg tccttcggcg tgtcccccat
    14221 ctcggcctcg atctcatcgc cgtcggcgag cgctacagcg ccggccggtt cgcggcggac
    14281 gccgccggct ggatcgggga cgtgcggcgc accgggctga cgccggtcgt cgtcggcggg
    14341 acggggttct acatccgggc gctgggcgac ggactcttcc aggagcccga cctcgatccg
    14401 gagcggcgcg aggcgttgcg tgattggacg gatgggttgg agacgggtgc cctggtccgc
    14461 tgggcggggc ggctcgaccc gcggttcgcg gggggcggcc ggcagcgggc ggcgcgggcg
    14521 atcgaggtcg cgctcctcac cggacacccg ctctcccact ggcaggaggt ggcccgggag
    14581 acgggtgcga tccggccgtg gtacattcaa ctcaccttgc cgcgcgacgt gcttcatcgc
    14641 cggatcgccg agcgggtgga ccggatgctc gagcgggggt tggtgaatga agtgaggggc
    14701 gtgctggcca ccggcgtggc gcccgatgct ccggggctcg atgcggtcgg gtatcgcgag
    14761 gtcgtggcgg cggagcgcgg cgagctgtcg tgggaggccg tgcgcgaaac gatcgtgatc
    14821 gccacgcggc agtacgcgaa gcggcaggag acgtggttca acaatcaggt gtcaggggtc
    14881 aggggccagg ggtcagtggc tccggtttgg aagctcgacg cgaccctgcc acccacagaa
    14941 ctggcacgac tggtgttcga tcgctggtcg tcgctgaccc ctgacccctg acccctggtc
    15001 cctgcatctc atgcgcatcg gtatcacttg ttacccgacc tatggcggtt ccggcgccat
    15061 cgcgaccgag ctgggcctcg agctcgcgaa gcgcgggcac gagatccact tcgtgtcgta
    15121 cgcgagccca ttccggctcc cgtcctttgc cgagcgggtg accttccatg aagtgacgca
    15181 ggcggagtat ccgctcttcg agacggcctc gccctacgcg ctggccctcg cggtgaagca
    15241 acacgaagtg gcgctgcggg agcagctcga catcatgcac gtgcattatg ccattccgca
    15301 cgcggccacg gcctggctgg cgaagcagat gctcgccgac cagcgggatc tcaagttggt
    15361 gaccaccctc cacggcacgg acatcacgct ggtggggaag gacccgagct attacacgct
    15421 caccaagttc tccatcgagc ggtccgaccg ggtgaccgcg gtgtccaagt atctccggga
    15481 agagacgtac cgggcgttcg gatgccagcg gtgcgacgtc accgtcatcc ccaacttcgt
    15541 ctccaccacc gcgtacaagg ccgcgaatga gagctggttc cggcgggcgt tgggcgccgg
    15601 cacgatgccg attctgctcc acgtctcgaa tttccgcccg gtcaagcggg tcgtggacgt
    15661 cgtacagatt ttcgccggag tccgccgggt gatgccggcg atgctggtga tggtgggtga
    15721 cggccccgat cgggacgccg cgcaggccga agccgatggg ttggggctca agtccgacgt
    15781 ccggttcctc ggcaaggtgg acaacgtggc cgacgtgatg cgcggggccg atctcttcct
    15841 gctgccgtcc gaagccgagt ccttcggcct ggcggcgctc gaggcgatgg cgtgcggcgc
    15901 gcccgtgatc gcctccaacg ccggcggcct tcccgagctc gtggtcgagg gtgagacggg
    15961 ctttctctcg gcgcccggtg cagtggacga catgaccggc aacgcgctca aggtcctcca
    16021 ggattcggcg ctggcggccc ggatgcgggc ggccgccgtg gaacgcgcgc aggcgttctc
    16081 ggtcgacaag gtcgtgcctc agtacgaacg gctctacgaa gatctcctcc gtggttgatc
    16141 tgctccgact ggttcgggcg cacaatctgg tggtcgcggg tctcggcgtc ttcgccggtg
    16201 ggtggatcgc gctcggtcgc gtcggcgctc ctggccctct cgtgtgggcc gtgctctccg
    16261 gggtcgggct cggggtggca gggaacgtcc tcaacgacat ctgggacgaa gccggggacc
    16321 gggccaatgc ccggaccgat cggcccctgg catctggccg cgttcgccgg gggagcgcgg
    16381 atctgctggt cttctggggc gcgttcctgg ggctggggac ggcggcgctc gtcagcggaa
    16441 cggtgttcgc gctcgcgggt ggcgcgctcg tgctgatggc ggcgtattcg ccctgcctca
    16501 agcgccgggg agtggccggg aacctgacgg tcgccctcgt ggcgggattc ccgctgatgt
    16561 atggcgccct cagtgtgggc cgcggcgccg cgggactcgt gccctgggtg ctcgcgggct
    16621 ggctgcactt tggccgtgag gtcgcgaagg atctggtcga cgtgcccggg gaccgcgtac
    16681 tggggcgccg gactgtcccg attctctcag gggaggcggt gggacggaac gtcgcgaagg
    16741 cgatcttgtg gagcttcatt cccgccagtc tggcccttcc ggcgctggcc ggatacggac
    16801 ggtggtattt cgccctggcg gttttcgctg acgtgctcgt gtggagtgcc ggccgcgccc
    16861 tctccgggca gcggtacccc gtcgccgtca ggcacgcgaa actcgccatg cctctcggcg
    16921 tggccgcact cgtactgggg agggtcgtgt gacctggtgg caaggcgtga ttctcgggct
    16981 ggtgcaaggc ctcacggagt tcctgccggt gtccagctcg ggacacctgc gcgtcatcga
    17041 ggtgctcacc ggcgtcaagg cgcccggcgt tttcgtcgaa gtctccttgc acgtcgccac
    17101 cctcttcgcg gtgctggtcg tgtacggtgc gagtctctgg agcatcgtcc ggggcacgct
    17161 gtccggtgac cgggcgtcgc tccggcgggc cgggttgctg gtgatcgcca cgattcccgc
    17221 ggcgatcctc ggcgtgctct tccacaagca ggtggaggag gccgccacca gcctcgtcct
    17281 catcgggatc tgcttcgtcg tcaccgggat cgccctctgg tcgacgaaag agcggcacgg
    17341 catgcgaccg gagccgaccg tcgcgggtgc cgtggtgatt ggtctcgcgc agtcggtggc
    17401 cgcgctcctg cgcggcatct cgcgctccgg ctctacggtc accgcggccc tgtgggtggg
    17461 cttggatccg gtcgatgcgg cggagttctc gttcctgctc tcgatcccga tcatcgccgg
    17521 cgctgcgttg gtggagggcc ggcacgcgac cgtggacatc gcggcggtgg gcgcggggcc
    17581 tctcgccctg tcgttcgcgg tcgcgttcgt ggcggggatc ttcagcatcc ggttcctggt
    17641 cgcgatgctc aagcgcggac gattttatct cttcgcgccc tactgctggg cggccggtct
    17701 cttcgtcatc gcctatgggc tgtggcatcc ctgaccctgg atggggtctc gggcggctcg
    17761 ctcgccaagc ggtggggcgt tccgcaagcc gggttgttcg attccctcgg ttccacgctc
    17821 gactcgattc acgagttggg gacctccggt gccccctcgg gaaccgtcgt catcgccgac
    17881 gtgcagacgg cgggacgggg gcgcgacgga cgcacgtggc ggtcgccgat gggtgggctc
    17941 tggctcggcg tgttgctgag gccgcggacg tccgatgtcg gggtctattc catccgggtc
    18001 ggactcgtgc ttgccgacgc gatcgacgag gtgttgggac gtcccgccgc ccgggtgaaa
    18061 tggccgaatg acatcgtcct ggccgaccgg aaggtggcgg gcattctgtg cgaagggcgc
    18121 tggcaagggg agaccctgca ctggctggcg gtcggcatcg gatgcaacgt cgcgaacgaa
    18181 ctccccccgg atgtcgccgg cctcgcgacg cggctcgccg atcaccggcc cggcgctacc
    18241 cggctcgacc tctgtgaccg gctggtaccg gcgctccccc gcctgggtgc cgccggcctc
    18301 cgcctcacgg aggcggagct cacggcattc gggtcgcgga actggctcgc ggggcggcag
    18361 atccgggcgc ccctcgccgg gaaagtcatt ggactcgaag gaaatggcgc cttgctcgtg
    18421 gaaacggccc ggggccgtca gaccgttcgg gaagggcacg tcgagcttgc ctgaggtaaa
    18481 ttagcgcccc atgctgctcg ccctcgacgt cgggaacacc gaaaccacgc tgggcttgtt
    18541 cgacggggac cagctcgcgc accattggcg tctcaccact acggtgcagc gcacgcctga
    18601 tgagtggggg gcggcgctca cttcctacct catgcaggcc ggccactcca ccggcgaagt
    18661 ccgcgccgcg atcgtcgcct cggtggtccc cctggtgtcc catgccttgg gggaggggat
    18721 ccggatcgcg atcggacagg ctcccacgtt cgttgacgcg cgttcccggc ttcccatcac
    18781 gctcgacgtg gatgagccgc tgacggtcgg tgccgatcgc atcgtgaaca cgctggcggc
    18841 ttcccaggtc tacaaggacg actgcatcgt cgtcgacttc ggcaccgcca cgactttcga
    18901 ctgcatcacc aaggacgggc gtttcatcgg tggcgtcatc atgccgggcg tcgtgacggc
    18961 cgcgaacgaa ctggtccgca agaccgcgaa gcttcccgcc gcggagctct cggcgccgcc
    19021 gcacgtgatc gggcgacgca ccgaggactg catccggtcg ggcgtgctgt tcggagcggc
    19081 cgaagcgatc gacggtctga ttcgccgggt acgcgcggag tggccggaag ggcggaagcc
    19141 gcgggcgatc gcgacgggtg gactcgccac gctcatcgcg cccctgtgca aggagatcgg
    19201 cggaaccgac cccgatctca cgctcacggg cctgcggctg gccgcggccg tgctggggct
    19261 caagtggtga ccgacgccca cgcgacgaag acactcaacg tctggcgccg ggccgggtac
    19321 ggcgcgctcc ccggggacgc gttctcttac atccttcata tgcggcccgc cgagtggccg
    19381 atcatggcgg cgcacacggt gttggggtac gtgctcgcgg tcggctggtc ggggctccgg
    19441 tcggggcagc agcttggctc cgcgctcttc gccctgttca tctgggtcat ctgcctgaac
    19501 ggcggcactc tcgcgatcaa tagtgtctac gacaaggatg agggggacgt cgggtacctc
    19561 aactcgcctc cgcctcttcc gaagcatctc ctcaccttca gctgggttct gctcgtcctc
    19621 ggccaggtgc tggccttccg cttgccctgg ggattccgga tcgcctacgc ggtctgtttc
    19681 gtgatgtcgg tggcgtattc ggtgcccccg ctgcggttca aggctgtcgc gggactggat
    19741 tgggtgatca acatgtgggg gttcgggacc ctgaccccgt acgccgcgtg ggccgccacc
    19801 ggccgtcccc tcgacttggg gcacgcggtc atctttctct cgttctgtgc gctgttcgcc
    19861 ggcctctatc cgctcaccca gctctatcag ctggaggagg acaagaagcg cggagaccgg
    19921 acgctggcgc tcatgttcgg gctggaggcg agcctgtggc tcgcgaccgg atcggtgatt
    19981 ctggcgttcg gcggcctgtc ctgggccgcg gacattctgc gccggtctgg aacgttgatt
    20041 ttctttgcgc tcgccgcctg gctggcggtg ctggtgccgt ggattcgggc ccgaaacgtg
    20101 tggacaccgc aacagcacca gcgcgggatg tattgggcgc tgggggcctg ggcggtgacg
    20161 gacgtggcgg tcctgctcgt ctttgcgcga taggcttgtt agattgaccg tcggacccct
    20221 ttgatcgagg agccttgctg atgtcggcga ccgccactcc caccatgacc gaccgtaccg
    20281 agctgccgca cgtcctgatc agcggcgcca agctgggcgt gctcaccgcg gtgtgggtcg
    20341 tgctctatct cgtgatctgg cggtacgttc cgggcaccgt cggcggcgtg ctcgacgccg
    20401 tgatcgtgct gctcgggggc ctgtcgatgg cgctcttgcc tgccgaatgg accggggcac
    20461 ggcacaccga agggatcgct ggggccgcgg gcgtcgggct cttcagtacc gtcgtattct
    20521 ccgcgatcga catcatcatt ctccggaacg tccacgcgta tccgtggact tgggacgcga
    20581 tcggcggcaa cagtacctgg tggtatctgc cgacctggtg gcagctgggc accttcgtgt
    20641 cgtggatggg cgccatgctg accgccggcc ggaattcgaa gcacgcggcg tccatgacca
    20701 gcctggccct cccgctcgtc gtcggcgccg tcgtgatcgg tgagatcgcc cacctgatgg
    20761 ggttcgggtt cacctcgacc tacccggtcg ccatcggcct cggattctcg gtcatcctca
    20821 ccgtccttgg agttgccgcc atcgcgcgga aggcgtgacg gacggaggcg tgccgcgctg
    20881 gctcgcgcac ttcctgctgg tcgtcggttg gctcctcacg cctctctgcg cgtggggagc
    20941 ttcttatttc gggctctgga tcggggccaa ggtcggcgtg cggttctcgg gaccgggggc
    21001 catggtgggg gtagcactgg gaatcgcatt tctgtccggc ttcgtgatgc tggccttgtg
    21061 ggtgcggatg atgcggcgtt tcccgcactg gctggcccac cggatggctc cccgcccgtc
    21121 acaggagaac cgtgcgacgg aatgacgtcg ggtttctgat cgtcacgctt tcgctggcgg
    21181 ccacttccct ggccgcgcag ggggagatcg agttccggta cgggcaatgg taccgggccg
    21241 gccacggcgc gacctatgag ttccgttcgc attctccact caccggtgtc atttcgcaga
    21301 ccttcggcgc gaccgtcatt gtcaacgatc gcttggggcg gcggcaggcc ttttacggcg
    21361 gcggatggga agcgcacctc ttccggggct cctacacgtt cgggccctat ggtctgctcg
    21421 gcgtcgcgct tggcctgtcg accgataccg ccaagcagga gctggcggcg ctgtggagtg
    21481 tcggcggtgg cctcgagtgg aggccgttcg accggatcgc acttggcgtg gaagggcgct
    21541 atcgcgacga ggatcggggg ccgcggggat tctggtcggg gggcggcagc ccccgaaagg
    21601 gattctcgct ctccctgggg ctcgccatcg gactcggccg acatggcggc aggatgtccg
    21661 ctccgtcaaa cccggtgctg cctccacccg cttcgaccgt ccccagcaat gccccggtgc
    21721 agcctccgac gaccatttca ggaaatgccg cggccgcgga tgtggttcgc acggccctcg
    21781 acgtgctggg caccccgtat caatggggtg gcacggcgtc gaacggattc gattgctccg
    21841 gcctgatcca gtacgcctac gggcagcatg gcgtgcggtt gccgagaatc agccgggacc
    21901 aggcgcgggc cggtgcggag gttacgccgg tggtggaggc gctccagccg ggtgacattc
    21961 ttctgttctc ggccagtccg ggtgccggcg tgacgcacgt gggcatgtac gtgggggagg
    22021 ggaagttcat tcacagcgcg agcagcggcg tgaagatctc ccggctcgac cggagcgact
    22081 ccgacggcgc ctattggatc ccgcgctggg tgggcgctcg acgcatcctt ccgtgagcgg
    22141 ccgctgggcg gtggccctcg gcctcgcgtt cgcgtgcgcg gggaatcccg gccccgccgg
    22201 tcctccaagt cccctgcgtg gttacgacat cgtgattcga gggcgcgatt ccctcagcca
    22261 cgccctgcag ggggcgttcg cgcgtgctgg atacaccgtg cgggacgacg tgcgcggggg
    22321 cggtcatatg gccgccgccc tggtttggtg gaagtacgtc gaccaggagg ggcgggggac
    22381 ccttgaggcc caggtggcgg atacccgccg ggggcatgtg cttgcggttg cgacgattcc
    22441 tgcggatacc ctccccggcg actggcaggg gcgcgctgag ctcctcgtaa aggccctcct
    22501 aaatccgtct ccttgacatg cggttcccgc ctgtcgataa ttcgtccggt tgctagcact
    22561 caccactcaa gagtgctaac gatatcagaa gcaatcactt agtccttgtg gaggcagagg
    22621 gttatggcga ccggaagcaa gggtgggaag cacaagctgc tcccgctggg tgatcgcgta
    22681 gcgatcaagc cggctgagga gacggagacg atgaaggggg gcctctacat ccccgatacc
    22741 gccaaggaaa agccgatcca gggtgacgtc gtcgccgtgg gtcccggccg gaccgaaaag
    22801 ggcgagaagg tgccgatgga gctgaaggtg ggtgaccgcg tgatctacgg aaagtattcc
    22861 ggcaccccgg tggagctcga cggtgaggaa gtcatcctca tcaaggaatc cgacgtcatc
    22921 gcgaagctcg gttaaggaga gaccaacaga tggctgccaa gtttctggag ttcaacgtcg
    22981 aggcgcgggc ccgcctgaag cggggtgtgg accagctggc cgacgccgtg aaggtgaccc
    23041 tcgggcccaa gggccggaac gtggtcatcg agaagaagtt cggcaatccg accgtcacca
    23101 aggacggtgt gacggttgcc aaggaaatcg agctcgagga cgagatcgag aacatgggcg
    23161 cccagatggt gaaggaagtg gcgaccaaga cctccgatct cgccggtgac ggcacgacca
    23221 ccgcgaccgt gctcgcgcag gcgatctacc gcgaaggcct gaagtcggtc accgcgggcg
    23281 tgaatccgat ggcgttgaag cgcggcatcg acaaggccgt cgagacgatc atcgaagagc
    23341 tgaagaggat ctccgtgccc accaagggcc ggaaggaaat cgcccaggtc ggcaccattt
    23401 cggccaacgg cgacaaggaa atcggcgaca agatcgccga cgccatggac aaggtgggca
    23461 aggacggcgt gatcacggtc gaagaggcga agggactcga gaccacgctg gagacggtgg
    23521 acgggatgca gttcgatcgc ggctacctct ctccctactt cgtcaccgat cccgagaaga
    23581 tggaagccgt gctcgaggac gcgtacgtcc tgatccacga caagaagatc tcgtcgatga
    23641 aggatctgct cccggcgctc gagaaggtcg cgcagtcggg caagcccctc ctcatcatcg
    23701 ccgaggacgt cgagggcgag gcgctggcga cgctggtggt caacaagctg cgcgggaccc
    23761 tcaaggtcgc ggcggtgaag gccccaggct tcggcgaccg ccggaaggaa atgctgatcg
    23821 acatcgagaa ggtcaccggc ggcaaggtcg tggcggaaga gaagggcatg aagctcgaga
    23881 acaccgtcct cggtgatctc ggccaggcca agcggatcgt ggtggacaag gacaacacca
    23941 ccatcgtcgg cggccgaggc aagcaggccg acattcaggg ccgcatcggc gagatccgcg
    24001 ccgcgatcga caagtcgacg tcggattacg acaaggagaa gctgcaggag cggctggcga
    24061 agctcgccgg cggggtcgcc gtgatcaacg tgggcgcggc caccgagacc gagatgaagg
    24121 agaagaaggc ccgggtggaa gatgcgctgc acgccacccg cgccgcggtc gaggaaggca
    24181 tcgtccccgg cggtggctcg gcgctcctgg tctgccagaa gagcctcgac aagctcaagg
    24241 gcggcgacga cgacgagaag atcggcgtcg atatcgtccg gcgcgccatc gaggagccca
    24301 tccggatgat cgcccagaac gccggcgcgg aagcgtcgat cgtggtgggc aaggtgaagg
    24361 agtcgaagga caagaacttc ggctacaacg cctcgacgaa tgagtacga
//
LOCUS       DASUBP010000241         9581 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_253596, whole genome shotgun sequence.
ACCESSION   DASUBP010000241 DASUBP010000000
VERSION     DASUBP010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9581)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9581)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9581
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_253596"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..922
                     /locus_tag="VFU75_09150"
     CDS             <1..922
                     /locus_tag="VFU75_09150"
                     /inference="COORDINATES: protein motif:HMM:NF044477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU5467046.1"
                     /translation="NMVEMVQCEREGKAGTPRDYPLARAEVTRLLDRLRETPLAARRE
                     IPGLNPQRADIILAGVAVVDRLAKRLGTQRVLVNDRGIRDGVLLALIDELPGMARPRR
                     VAPPDRMESARRLARQCRSNERHCERVAALSVQLFDALRRPFDLPHEGREILMAGALL
                     HDIGYLINHERHHKHAYHLIMHGDLPGFSAREVELIANVARYHRRAAPKKSHANFEKL
                     SKDERRLIRRLSGILRIADGMDRTHGGVIKTVRARTDRKQVRISVTASRNPSIELDDA
                     RRKSALFEKAFDRSLVFSMPRAPGGARRRA"
     gene            919..3105
                     /gene="ppk1"
                     /locus_tag="VFU75_09155"
     CDS             919..3105
                     /gene="ppk1"
                     /locus_tag="VFU75_09155"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003921.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HEU5467047.1"
                     /translation="MTDRAPAAFPADHYINRELSWLEFNARVLEEAADPTNPWLERLK
                     FTSIFSSNLDEFFEIRVSGLQQQVYAGVEPQDFGAEGMAPAEQLAAIERRTHELVAQQ
                     YRVLMTEAFPGLAEVGIERVRWADLTPAERRHAVGFFRASVFPVLTPLAIDPGHPFPH
                     VHNKSLNIALLVERKNGGLPRRHFAVVQVPAVLDRVMIVSRAEKKVRFILLEDVIAEH
                     LGELFGHLKVVNHTVFRVTRNNDLSIEEDEAEDLLETIEESLRQRMRSDPVRLEISAD
                     ASDLFVDMLTEAHDLEPRDVYRVDGPVDLTALMALYRLEGFGAAKDEPLVPRVPPPFA
                     AADDLFQVIRGRDVLVHLPYESFNCVVDFINQAADDPQVLAIKQTLYRTSGGSPIIAA
                     LARAAQNGKQVTALVELKARADEQANIAWARMLEQAGVHVVYGIVGLKTHCKAALVVR
                     REAEGIRRYVHLSTGNYNPATARLYTDLGLFTADPDFGEDVSALFNLLTGYAEGYEWK
                     KLLVAPIDLRERIISLIEREEQHAKAGRPARIIVKMNALVESSVIDALYRASIAGVQI
                     DLVIRGICCLRPGLPGVSERIRVTSIVDKFLEHSRIFYFENGGAPDVWLASADWMPRN
                     FWRRIETVFPVEDANLKLRIVNDILRLVLADNVKARELRADGTYRRRAPTNGETPVRS
                     QVALQLLAREASREKKSDTRPKLVPVVRRRPDTDPAPGSGRTRTRR"
     gene            3191..4210
                     /locus_tag="VFU75_09160"
     CDS             3191..4210
                     /locus_tag="VFU75_09160"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HEU5467048.1"
                     /translation="MPAWEGVSELARAYLDDARRAAPGLGTRPDPDALHDFRVALRRL
                     RGLLRAYRAPLGPRIPKRLRRRLSAVADSTGAGRDADVMLTWIAGQMKGLSRSERVGA
                     RWLAERIKRRRDADPEARRLPGRFDRLDRRLRKRLTAEARTAGAPVGPSFAGTTARLV
                     TDAAVSLAEALREIRKPEDTEAAHHARLAGKRLRYLLEPVADSAAGAPALIERLRGLQ
                     ELLGELDECQVMLDEIREAVEALGASRAREAHDLQVEGKPRPRLKRAREDSGLLALAR
                     RARARRDDLFFVLQPQWLGDRAAEVIPLVREGVATLTARRPAPYFMAPRIRRYRSRRS
                     APART"
     gene            4324..5418
                     /locus_tag="VFU75_09165"
     CDS             4324..5418
                     /locus_tag="VFU75_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467049.1"
                     /translation="MKNAIWTVPQFTSLEGFLNASLRRFMLTAGLGALVAAPAVAQNP
                     APAPQVTVGGVVYGQYFYQLKDTAAHFNAFDVTRAYVNVIGKFSGGIQTRVTLDIAHA
                     VAGQQTYRLKYAFAGWTPEGSALTYKLGLFTTPWLDWEEALWDYRMEGATVFDRPGYL
                     SSSNFGIGADGNINKEQVDFQAGVFDNGSYSAGLGDQRKNVQGRLSVRAMDTDDPSRV
                     GGLRITAFGQVGKPVTGGTQTRFIGMVSYKSKALTLAAEGGITDDSAAASVAPVKGQV
                     ISAFGVYHFTDSPVAVIARFDNIKPNKDAPSTTPGFTNTRIIAGVSYQLTSNVRLLGD
                     IDFLSYKNGSPSPAAEAARELAQFQAQFTF"
     gene            5437..6504
                     /gene="pstS"
                     /locus_tag="VFU75_09170"
     CDS             5437..6504
                     /gene="pstS"
                     /locus_tag="VFU75_09170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEU5467050.1"
                     /translation="MTKHLTVAVLAIGAITAVSTPGAAQTKITGAGSTFSTILYTDWM
                     QTYNKAHADVQLNYQSIGSGGGIRQFSDKTVDFGASDAPMTDSAMAAIQGNVLHIPTE
                     MGGVPPTYNIPGVTAQLKFSGPVLAGIFLGTITKWNDARIAADNPGVKLPDQDIVVVH
                     RSDGSGTSFIFTDYLSKVSPDWASKVGKGTAVNWPVGLGAKGSEGVSATVQQTPGAVG
                     YVELGYALANHLPVGAVKNKAGKFITASAATVTAAAAGAMKDMGPTTDFRVSITNADG
                     PEAYPISSFTWMLVHKNYDDAAKAKAIVAYTWWALTAGQVRAEPLGYAPLPKAMRPWL
                     EARLKTITAGGQAVWSGPAGR"
     gene            6640..7656
                     /gene="pstC"
                     /locus_tag="VFU75_09175"
     CDS             6640..7656
                     /gene="pstC"
                     /locus_tag="VFU75_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEU5467051.1"
                     /translation="MPTTFPQTIASTTGESIAPISTLSAAPRPTRRYYGDRIYRWLLT
                     CLAMTLPLLLVTIVAELLVSAWPSIHKFGFSFIWTSVWDPVGEVYGAAPMIFGTLVSS
                     LLALVIAVPLSLGVAIFLTEFAPKWLRQPVAFLVELLAAIPSVVYGLWGIYVLIPLLR
                     DYAAPPIRALLGWTPLFSGVFYGNSMLAGGIILAIMIVPYIAAISREVLLAVPATQRE
                     AALGLGATRWEAVWGAVLPYGRAGIIGAVMLGLGRALGETMAVTMVIGNRHDIALSLF
                     QPGYTMAAAIANEFSEATSTMYISALFEVGLVLFVITIGVNAFARLLVWRVARGTAVG
                     SKAL"
     gene            7653..8495
                     /gene="pstA"
                     /locus_tag="VFU75_09180"
     CDS             7653..8495
                     /gene="pstA"
                     /locus_tag="VFU75_09180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEU5467052.1"
                     /translation="MSVRIATRRIRSGFFTGLVVLLCFLAVLPLFLIFADLVHKGASS
                     INWDFFVKIPVPAGQSGGGVSNAIAGTIIIVLIACVIGIPIGIGTGLYLAEYGIGRLG
                     WFVRFLADVLNGTPSIVVGIFAWTWFVKPVHHFSALAGGAALALLMVPMLARTTEEML
                     RLVPNSLREAALALGYPRWRTSLSVMARVALSGIVTGSLVGVARVAGETAPLLFTALG
                     NINFDTNIKEPMQTLSLQIFTYATGPFEDWHRLAWASALVLMSLVLSLALAARWATRK
                     RYGT"
     gene            8503..9255
                     /gene="pstB"
                     /locus_tag="VFU75_09185"
     CDS             8503..9255
                     /gene="pstB"
                     /locus_tag="VFU75_09185"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004060638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HEU5467053.1"
                     /translation="MQLETRRLSALFSRNVIVREVSLPFPRHTVTAIIGPSGCGKSTF
                     LRCLNRMHELSEGGWATGQVMLDGHDIYGGGIEAVDVRRRVGMVFQRPTPFPTMSIYD
                     NVAAGLRVNGRIPRKQMDEVVERALTQAALWDEVKDRLKGSAVALSGGQQQRLCLARA
                     VAPAPEVLLLDEPTASLDPAGTQRIEELCTELKKRYTIVIVTHNMQQAARVSDFTAFF
                     YMGRLIEVGATQQMFTKPNDPQTEAYITGRFG"
     gene            9252..>9581
                     /locus_tag="VFU75_09190"
     CDS             9252..>9581
                     /locus_tag="VFU75_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5467054.1"
                     /translation="MTTPMAPVPSVQPHTPSAGSGSPPVLDVRQYAFWYGPKQVLFDL
                     ALTIPRQAVTALIGPSGCGKSTFLRSINRMNEIQAGTRHTGDILLEGVSVFSPGMDPV
                     DLRRQVGM"
BASE COUNT         1562 a         3319 c         3073 g         1627 t
ORIGIN      
        1 gaacatggtc gagatggtgc agtgcgagcg tgaggggaag gcgggcaccc cccgcgacta
       61 cccgctggcg cgcgccgaag tcacccgcct gctcgatcgc ctgcgcgaaa ctccgctcgc
      121 cgcccgtcgc gaaatcccgg gactcaaccc gcagcgcgcg gacatcattc tcgcgggtgt
      181 cgccgtagtc gatcgcctcg ccaagcggct gggcacgcaa cgcgtcctcg tgaacgatcg
      241 cgggatccgg gacggcgttc tgctcgccct gatcgacgag ctccccggga tggcccggcc
      301 ccggcgcgta gcaccgccag accggatgga gtcggcgcgc cgtctcgccc gtcagtgccg
      361 ttcgaacgag cgccactgcg agcgcgtcgc agcgctctcg gttcagctct tcgatgcctt
      421 gcgccggccc ttcgaccttc cccatgaagg gcgggaaatc ctgatggccg gtgccctgct
      481 gcacgacatc ggctacttga tcaatcacga acgccaccac aagcacgcct accacctcat
      541 catgcatggt gacttgcccg ggttctcggc ccgtgaggta gagctcatcg ccaacgtggc
      601 gcggtatcat cgccgggcgg cacccaagaa gtctcatgcc aatttcgaaa agctgagcaa
      661 ggacgaacgc cggctcattc gccggctgtc gggcatcctg cgcatcgcag acggcatgga
      721 ccggacgcat ggcggcgtga tcaagactgt gcgcgcccgc accgaccgga agcaggtccg
      781 catctcggtc accgcgtccc ggaacccgtc catcgagctg gacgacgcgc ggcggaagag
      841 cgccctcttt gaaaaggcct tcgatcgctc gctggtgttc tcgatgccgc gggcccctgg
      901 cggcgcccgg agacgtgcat gaccgaccgc gcccccgccg ccttcccggc cgaccactac
      961 atcaaccgcg agctctcgtg gctggagttc aacgcccggg tgctcgagga agcggccgac
     1021 cccacgaatc cgtggctcga gcggctcaag ttcacgtcga tcttcagctc caacctggat
     1081 gagttcttcg agatccgggt gtcgggcctg cagcaacagg tctacgcggg cgtcgagccc
     1141 caggacttcg gtgctgaggg catggcgccc gccgagcagc ttgccgccat cgagagacgg
     1201 acccacgagc tcgtcgccca gcagtaccgg gtcctcatga ccgaagcgtt tccggggctg
     1261 gccgaggtgg ggatcgagcg cgttcgctgg gcagacctca cccccgccga gcggcggcac
     1321 gcggtgggat tcttccgggc gagcgtattt ccggtgctca cgccgctcgc catcgacccc
     1381 gggcatccgt tcccccacgt ccacaacaag agcttgaaca tcgccctcct ggtggagcgg
     1441 aagaatggcg gtctgccccg gcggcacttc gccgtagtgc aggtaccggc cgtattggac
     1501 cgcgtcatga tcgtgtcccg cgccgagaag aaggtccggt tcattctcct cgaagacgtc
     1561 atcgccgagc acctcggtga gctgttcgga cacctgaaag tcgtgaatca caccgtcttc
     1621 cgggtcaccc gcaacaacga cctgtcgatt gaggaagacg aagccgaaga ccttctcgag
     1681 accatcgaag agagcctgcg ccagcgcatg cgctccgacc cggtgcggct cgagatctcg
     1741 gcggacgcga gcgatctgtt cgtggacatg ctcaccgaag cgcacgacct cgagccgcgt
     1801 gacgtgtacc gggtggatgg accggtggac ctcacggccc tgatggcgct gtaccggctg
     1861 gagggatttg gggccgccaa agacgaaccg ctggtgcccc gcgtgcctcc tcccttcgcc
     1921 gcggcggacg acctcttcca ggtgattcgc gggcgggacg tgctggtcca tctgccctac
     1981 gaatcgttta actgcgtggt ggacttcatc aaccaggcgg cggacgatcc tcaggtcctg
     2041 gccatcaagc agaccctgta ccgcacgtcg ggcgggagcc ccatcatcgc ggcgctcgcg
     2101 cgagccgcgc aaaatggcaa gcaggtgacg gcactggtcg agctgaaggc gcgcgcggac
     2161 gagcaggcga acatcgcatg ggcgcgaatg ctggagcagg ccggcgtgca cgtggtatac
     2221 ggcatcgtcg ggttgaagac ccactgcaag gcggcactcg tggtgcgccg cgaggcggag
     2281 gggatccgcc ggtacgtgca tctctcgacc ggcaactaca acccggccac cgcccgtctc
     2341 tataccgacc tgggtctctt caccgccgac ccggatttcg gcgaagatgt gagcgcgctc
     2401 ttcaacctcc tcaccgggta tgccgaaggc tacgagtgga agaagctgct cgtcgcgccg
     2461 atcgacctcc gtgagcgcat catcagcctg atcgagcggg aagaacagca cgcgaaggcg
     2521 ggccgaccgg cgcgcatcat cgtcaagatg aacgcgctcg tggagtcctc cgtcatcgac
     2581 gcgctgtatc gggcttcgat cgccggagtc cagatcgacc tggtgatccg cggaatctgc
     2641 tgcctgaggc ccggattgcc gggtgtatca gagcgcatcc gcgttacgag catcgtggac
     2701 aaattcctgg agcacagccg catcttctac ttcgagaatg gcggcgcgcc cgacgtgtgg
     2761 ctcgcgagcg cggactggat gccccggaat ttctggcgcc gcatcgaaac cgtcttcccg
     2821 gtggaggacg ccaacctcaa gctgcgcatc gtcaacgaca tcctgcgttt ggtgctcgcc
     2881 gacaatgtca aagcgcgaga actgcgggcg gacgggacct atcggcgccg cgcaccgacc
     2941 aatggcgaaa ctccagtgcg ctcgcaggtt gcgctgcagc tcctggcccg cgaggcctca
     3001 cgggaaaaga aatccgacac gaggcccaag ttggtgccgg tagtgcgccg ccgtcccgac
     3061 accgatcccg cccccgggtc cggacgcacc cgcacgcgac gctgatgcgc gacacaggcg
     3121 aacccttctg ggcgaccgcg cttcaagggg agaacccctc gtgaccgctt ttccacgcga
     3181 cctccccggc atgcctgcct gggaaggcgt gagcgaattg gcccgcgcct acctggacga
     3241 cgcgcggcga gccgcgcccg gtctgggcac ccgtcccgac cccgacgccc tgcacgactt
     3301 ccgggtcgcg ctccggcggc tccgcgggtt gctccgtgcc taccgcgcgc ccctgggtcc
     3361 ccgcattccc aaacggctgc gccgccgtct ctcggccgtc gccgactcga cgggtgccgg
     3421 tcgcgatgcg gacgtgatgc tcacgtggat tgcgggtcag atgaagggtc tcagccggtc
     3481 agagcgcgta ggcgcccgct ggctggcgga gcgcatcaag cggcgccgcg acgccgatcc
     3541 tgaggcccgt cggctccccg gccgattcga tcggctcgac cgtcgcttgc gaaagcgcct
     3601 cacggcggag gcccgcaccg cgggagcccc ggtcggcccc tcgttcgccg gcaccaccgc
     3661 gcgcctggtc accgacgccg cagtctccct ggccgaggcc ctgcgcgaga tcaggaaacc
     3721 ggaggatacg gaagccgctc atcacgcgcg gctcgcgggc aagcgcctgc gctacctgct
     3781 ggagcctgtc gccgactcgg ccgccggagc gcccgccctc atcgagcgcc ttcgtgggct
     3841 ccaggaattg ctgggagagc tcgacgaatg ccaggtcatg ctggacgaga tccgcgaggc
     3901 cgtggaagcc ctcggtgcct cccgcgctcg cgaggcacat gacttacagg tggaggggaa
     3961 gccgcggccg agactgaagc gggcccgcga agactcgggg ctgctggcgc tggcgcggcg
     4021 cgcccgggcc cgccgggatg atctcttctt cgtcctgcag ccccagtggc tgggtgaccg
     4081 cgccgccgag gtgatcccgc tggtccgtga gggcgtcgcg acactgaccg ctcggagacc
     4141 agccccatac ttcatggctc cccggattcg gcgttaccgg tcccggcgct ctgcccctgc
     4201 gaggacgtaa gccggctcgc ccgttccgaa gcgcccctgg tttcccgccg agacgttccg
     4261 ttacccaacc gttacgcttt ccgccagact ctgtgatttg gagaagcgtt tgtatcaccc
     4321 gaaatgaaga acgccatttg gaccgttccc caattcacct ccctggaggg attcttgaac
     4381 gcctcacttc gccgcttcat gctcaccgcc ggactggggg cgctcgtcgc cgctccggcc
     4441 gtcgcgcaga acccggctcc cgcgccccag gttaccgtcg gcggcgtggt gtacgggcag
     4501 tatttctatc agttgaagga cacggccgcc cacttcaacg ccttcgacgt gacgcgggcg
     4561 tatgtgaacg tgatcggcaa gttctccggc ggcatccaga ctcgcgtgac cctcgacatt
     4621 gcgcacgccg tcgccggcca gcagacctac cgcctgaagt acgcgttcgc cggctggacg
     4681 cccgaaggca gcgccctgac gtacaagctg gggctcttca ccacgccgtg gctcgactgg
     4741 gaggaagcgc tctgggacta ccggatggag ggcgccacgg tcttcgaccg gcccggatac
     4801 ctctcgtcct cgaatttcgg catcggagcc gacggcaaca tcaacaagga acaagtggac
     4861 tttcaggccg gtgtcttcga caacggcagc tactccgccg ggttgggcga ccagcggaag
     4921 aacgtccagg ggcgtctctc cgtacgggcc atggatacag acgaccccag ccgggtcggc
     4981 ggcctccgca tcaccgcctt cggccaggtc ggcaagcccg tcacgggcgg cacacagacg
     5041 cgctttatcg gcatggtctc ctacaagtcg aaagccctaa ccctcgcggc ggagggcggc
     5101 atcaccgacg attcggccgc cgccagcgtc gcgccggtga agggccaggt gatcagcgcc
     5161 ttcggcgtct atcacttcac ggactcgccg gtggccgtga tcgcacgctt cgacaacatc
     5221 aagccgaaca aggacgcgcc gtcgaccacc ccggggttca ccaatacccg catcatcgcg
     5281 ggggtgtcgt accagctgac gagcaacgtt cggctcctcg gtgacatcga ctttctttcg
     5341 tacaagaacg gttcaccgag cccagcggcc gaagcggcac gcgagctcgc ccagttccaa
     5401 gcccagttca cgttctaagc ggggagacga cacgacatga cgaagcacct gaccgtcgca
     5461 gtgctcgcca tcggggccat cacggccgtt tcgacccctg gggcagcaca gaccaagatc
     5521 accggtgcgg gatcgacgtt ctccacgatc ctgtacaccg actggatgca gacgtacaac
     5581 aaggctcacg ccgacgtgca gctcaattat cagtcgatcg gctcgggtgg cgggatccgg
     5641 cagttttcgg acaagaccgt ggatttcggc gcttccgacg cgcccatgac tgactcggcc
     5701 atggcggcga ttcagggcaa cgtgctccac atccccaccg agatgggcgg cgtaccgccg
     5761 acgtacaaca tcccgggcgt caccgcgcag ttgaaattct ccggcccggt gctcgcgggc
     5821 atcttcctcg gtacgatcac gaagtggaac gatgcgcgca tcgcggcgga caaccccggt
     5881 gtgaagctgc ccgatcagga catcgtggtc gtgcatcgat cggatgggtc cggcaccagc
     5941 ttcatcttca cggattacct gtccaaggtg agccccgatt gggcttcgaa ggtcggcaag
     6001 ggcacggccg tgaactggcc ggtcggcctg ggcgccaagg gcagcgaagg cgtctccgcc
     6061 acggtccagc agacgcccgg cgccgtcggg tacgtcgagc tgggctatgc gctggccaat
     6121 cacctccccg tgggagcggt gaagaacaag gccggcaagt tcattacggc cagcgcggcg
     6181 acggtgacgg ctgccgctgc cggagcgatg aaggacatgg gcccgacgac ggacttccgc
     6241 gtgtccatca cgaacgcgga cggccccgag gcctatccca tttcgagctt cacgtggatg
     6301 ctggttcaca agaactacga cgacgccgcc aaggcgaagg cgatcgtggc gtacacctgg
     6361 tgggccttga ccgccgggca ggttcgggct gaacccctgg gctacgcacc gctgcccaag
     6421 gcgatgcggc cctggttgga agcgcggctc aagaccatca cggccggtgg gcaggccgtg
     6481 tggagcggcc cggcaggccg gtaactccct ccaaccgact gtcggcgcta cccccgaagg
     6541 ttggcttact tcgggggttt cgccgtcacg gtggggtgac gcaatcgtta caatgccgtg
     6601 atggtggggg gagccacccc cgatagactc tcccccgcga tgcccaccac gtttccccag
     6661 accattgcct cgacgactgg cgagtccatc gctcccattt ccactctcag cgccgcgccc
     6721 cgtcccaccc gccgctacta cggggaccgg atctatcgct ggctgctgac gtgcctcgcc
     6781 atgacgctgc ccctgcttct ggtcaccatc gtggcggagc tcctggtcag cgcctggccc
     6841 tccatccaca agttcggctt ctcgttcatt tggacctcgg tctgggaccc cgtcggcgag
     6901 gtgtatggcg ccgcgcccat gatcttcggc accctggtct cttcgttgct ggcgctcgtc
     6961 atcgccgtac ccctgtcgct cggcgtggcg attttcctca ccgagttcgc tcccaagtgg
     7021 ctccggcaac ccgtggcctt cctggtcgag ctcctggctg ccattcccag cgtggtctac
     7081 ggtctctggg gcatctacgt cctgatcccc ctgctccgcg actatgcggc cccgccgatc
     7141 cgcgccctcc tgggctggac cccgctgttc agcggcgtct tctacggcaa ttcgatgctg
     7201 gcgggcggca tcatcctcgc gatcatgatc gtaccgtaca tcgccgcgat ttcgcgcgag
     7261 gtcctcctcg ccgttcccgc cacgcaacgg gaagcggccc tgggactcgg cgccacgcgc
     7321 tgggaagccg tgtggggcgc cgtcctgccc tacggccgcg ccggcatcat cggcgccgtc
     7381 atgcttgggc tcggccgcgc gctgggggag accatggcgg tgaccatggt catcggcaac
     7441 cgtcacgaca tcgccctctc gctcttccag cccggctaca ccatggccgc ggcgatcgcc
     7501 aacgaattca gcgaagcgac gtcgacgatg tacatctccg cgctcttcga ggtcggcctg
     7561 gtcctgttcg tgatcacgat cggcgtcaac gccttcgccc gcctgctggt ctggcgcgta
     7621 gcgcgcggca ccgccgtcgg gagcaaggcg ctgtgagcgt ccgcatcgcg acccggcgga
     7681 tcaggagcgg cttcttcacc gggctggtcg tactcctgtg cttcctcgcc gtcctgccgc
     7741 tgttcctgat cttcgcggac ctggtgcaca agggcgcgtc atcgatcaac tgggactttt
     7801 tcgtgaagat cccggtgccc gcgggtcagt cgggtggcgg cgtctcgaac gccatcgccg
     7861 gaaccatcat catcgtcctg atcgcctgcg tcatcggaat tcccatcggc atcgggaccg
     7921 ggctctatct ggccgagtac ggcatcggac ggttgggctg gttcgtgcgg tttctggccg
     7981 acgtcctgaa cggcacgccg tcgatcgtgg tgggcatctt cgcgtggacc tggttcgtga
     8041 agccggtgca ccacttctcc gcgcttgccg gcggagcggc gctcgccttg ctgatggtcc
     8101 cgatgctcgc ccgtaccacc gaggaaatgc tgcgcctggt gccgaactct cttcgggaag
     8161 cggctctggc gctgggatac ccgcgctggc ggacgtcatt gtccgtgatg gcgcgagtgg
     8221 ccctgtcggg gatcgtgacc ggttcgctgg tgggcgtggc ccgggtggcc ggcgagacgg
     8281 ctccactgct cttcacggcg ctcgggaaca tcaacttcga cacgaacatc aaggagccga
     8341 tgcagacgct ttcgctccag atcttcacct acgcgacggg accgttcgag gattggcacc
     8401 ggctcgcgtg ggcgtccgcc ctcgtcctca tgagcctcgt gctcagcctt gcgctggcag
     8461 cccgctgggc cacccgcaag cggtatggca cctgagactc tggtgcagct agagacccgc
     8521 cgcctctccg cgcttttcag ccggaatgtg attgtccggg aggtgagcct cccgttcccg
     8581 cgacatacgg tgacggccat catcggtccg tccggctgcg ggaagagcac gttcctgcgc
     8641 tgcttgaacc gcatgcacga gctgtccgaa ggtggctggg cgaccggtca ggtgatgctg
     8701 gatggacacg acatttatgg cgggggcatc gaggcggtgg acgtgcgccg ccgggtcgga
     8761 atggtgttcc agcgccccac gcccttcccc accatgtcca tctacgacaa cgtcgccgcg
     8821 ggcttgcgcg tgaatgggcg catacccaga aagcagatgg atgaggtcgt cgagcgtgcg
     8881 ctcacccagg cggcactctg ggacgaggtg aaggaccggc tcaagggcag tgccgtcgcc
     8941 ctttccggcg gccagcagca gcgcctctgc ctggcgcgcg ctgtagcacc cgcacccgaa
     9001 gtcctgttgc tcgacgagcc gaccgcctcc ctcgaccccg cgggcaccca gcggatcgaa
     9061 gagctctgca ccgagctcaa gaagcgctac acgatcgtga tcgtgaccca taacatgcag
     9121 caggcagcgc gggtctcgga cttcaccgct ttcttctata tggggcgcct gatcgaagtc
     9181 ggcgcgacgc agcagatgtt caccaagccg aacgatccgc agaccgaggc atacatcacc
     9241 ggacggttcg gatgaccacc cccatggctc cagtcccgtc ggtccagccg cacacgccgt
     9301 ccgcgggcag cggttcgccg ccggtgctgg atgtgcgcca atacgcattc tggtatggac
     9361 cgaagcaggt gctcttcgac ctggccctga ccatcccgcg gcaggccgtg accgccctga
     9421 tcggcccgtc ggggtgcgga aagagcacct tcctccggtc catcaaccgg atgaacgaga
     9481 tccaggcggg gaccaggcac accggggaca tcctgctgga gggcgtgtcg gtcttctcgc
     9541 ccggcatgga cccggtcgac ttgcggcggc aggtgggcat g
//
LOCUS       DASUBP010000242         2161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_785243, whole genome shotgun sequence.
ACCESSION   DASUBP010000242 DASUBP010000000
VERSION     DASUBP010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_785243"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..436
                     /gene="rpsO"
                     /locus_tag="VFU75_09195"
     CDS             179..436
                     /gene="rpsO"
                     /locus_tag="VFU75_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEU5467055.1"
                     /translation="MYDKTTPVRKHGQHDKDTGSAPVQIALLTERINSLTGHFQTHAK
                     DHHGRRGLLRMVHHRRRLLEYLKRTDLERYRRLITELDLRH"
     gene            527..>2161
                     /locus_tag="VFU75_09200"
     CDS             527..>2161
                     /locus_tag="VFU75_09200"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEU5467056.1"
                     /translation="MKHVEKQFAGRTLRLESGRLAKQAAGSCLMQFGETVVLATVTVS
                     DNVSTLPFFPLTVEYREKSYAAGKIPGGFLKREGRPSDEEILSARVTDRSIRPLFPEG
                     FKNEVQVFIYVLSADQENDADVLGVTAASTALSLSKVPWNGPIAAVRVGRVEGAWVLN
                     PTFQQLEFSDVDMIVTGSADSIVMVEGGTLELSEADIVKGLQTAHDGIKDLIATQKEL
                     IAETAQPKMEWTKVEAPAELVARVKALAEPRISEALNLAEKAERTQALAALKHTIQEQ
                     LAEEFPENGKDIANTIEEIEYHTMREQVLSRGERVDGRDLDTVRPITCEVGVLPRTHG
                     SALFTRGQTQALVSATLGSVSDEQRIDSIDVPQETTKSFMLHYNFPPFSTGEVKMVRG
                     VSRREIGHGALAERALQALLPPYEQFPYTIRIVSDILESNGSSSMATVCGGSLALFDA
                     GVPMTGACAGVAMGLIKEGKRVAVLTDILGTEDHLGDMDFKVAGTRAGVTSIQMDIKI
                     DGLDFGIVTEALEKARKARLHILDIMEAAIPQPRSQL"
BASE COUNT          404 a          729 c          698 g          330 t
ORIGIN      
        1 tccttctccc gggcggcaaa gacccaggcg aaaccgccct gtcccagcag ttgttcgatg
       61 gcgtatgggc ccaacgtctg gccgacgagg tcggtcgggg cgttcgtcat gaggccttta
      121 cagaggcgcg atgagcctct aacttactgt gctcacaacg attagggaac acgaacagat
      181 gtacgacaag acgacgccgg ttcggaaaca cgggcagcac gacaaggaca ccggatctgc
      241 tccggtgcag atcgcgctgc tcaccgagcg catcaacagc ctcaccggac atttccagac
      301 ccacgccaag gatcaccacg gacgccgcgg cctgctgcgc atggtgcacc accgccgccg
      361 cctcctggag tatctgaagc gcacggatct ggaacggtac cgccggctca ttactgagct
      421 ggacctccgc cactaagcgg tcggaacagg cgtcgcgcgg ggttggccgg gaagagcacc
      481 ggcctagccc ggcgtccttc tattgtgctc tagacaggcg accgaagtga agcacgtcga
      541 aaagcagttc gccggccgga ccctccggct ggagtcggga cggctggcca agcaggccgc
      601 cggatcctgc ctgatgcagt tcggggaaac ggtggtcctg gcgaccgtca cggtctccga
      661 caacgtctcc accctcccgt tcttccccct caccgtcgaa taccgcgaga agagctacgc
      721 cgccggcaag attcccggcg gctttttgaa gcgcgaaggc cgcccctcgg acgaggaaat
      781 cctctccgcg cgggtgaccg accgctccat ccgcccgctc ttccctgaag gcttcaagaa
      841 cgaagtccag gtcttcatct acgtcctctc cgccgaccag gagaacgacg ccgatgtgct
      901 gggcgtgacc gccgcgtcga cggcgctctc cctctccaag gtgccctgga atggtccgat
      961 cgccgcggtc cgcgtgggcc gggtcgaggg cgcctgggtg ctgaacccga ccttccagca
     1021 gctcgagttc tcggacgtcg acatgatcgt cacgggctcc gccgactcca tcgtgatggt
     1081 ggaagggggc acgctcgagc tgtccgaggc cgacatcgtg aagggcctcc agacggcgca
     1141 cgacggcatc aaggatctca tcgccacgca gaaggagctg atcgccgaga cggcgcagcc
     1201 caagatggag tggaccaagg tcgaggcgcc cgccgagctg gtcgcccgcg tgaaggccct
     1261 ggccgagccg cggatctccg aagcgctgaa cctcgcggag aaggcggagc ggacgcaggc
     1321 gctcgcggcg ctgaagcaca cgattcagga acagctggcg gaggagttcc cggagaacgg
     1381 gaaggacatc gccaacacca tcgaggaaat cgaataccac accatgcggg aacaggtgct
     1441 ctcccgcggt gagcgggtgg acggccgcga tctggacacc gtgcggccca tcacctgcga
     1501 ggtcggggtc ctgccgcgca cccacggctc cgcgctcttc acccgcggcc agacgcaggc
     1561 gctggtgtcg gcgaccctgg gcagtgtgtc ggacgagcag cggatcgact cgatcgacgt
     1621 tccgcaggag accaccaagt cgttcatgct gcactacaac ttcccgccct tctccacggg
     1681 cgaagtgaag atggtgcgcg gcgtctcccg ccgggaaatc gggcacgggg cactggccga
     1741 gcgcgccctc caggcgctgt tgccgcccta tgagcagttc ccgtacacca tccggatcgt
     1801 ctccgacatc ctcgaatcga acggctcgtc gtcgatggcg acggtctgcg gtggctcact
     1861 cgccctgttc gacgccggcg tgcccatgac gggcgcctgc gcgggcgtgg cgatgggctt
     1921 gatcaaggaa gggaagcgcg tcgcggtgct caccgacatc ctgggcaccg aagaccacct
     1981 cggcgacatg gacttcaagg tcgcggggac gcgggccggc gtgacgtcca ttcagatgga
     2041 catcaagatc gacggcctgg atttcgggat cgtcaccgag gcgctggaga aggccaggaa
     2101 agcccggctc cacatcctcg acatcatgga agccgccatc ccgcagcccc gctcgcagct
     2161 g
//
LOCUS       DASUBP010000243         9865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_866742, whole genome shotgun sequence.
ACCESSION   DASUBP010000243 DASUBP010000000
VERSION     DASUBP010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9865
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866742"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VFU75_09205"
     CDS             complement(<1..326)
                     /locus_tag="VFU75_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HEU5467057.1"
                     /translation="MSPTLFFIVTSLIKMIVVFTVIMVGVAFMTLVERRLCAFMQDRL
                     GPNRVGPFGLLQPAADGLKNIVKEETYPARADKWLFILAPAISFTPALLTFAVIPFAA
                     PLATKWG"
     gene            complement(323..1864)
                     /locus_tag="VFU75_09210"
     CDS             complement(323..1864)
                     /locus_tag="VFU75_09210"
                     /inference="COORDINATES: protein motif:HMM:NF024900.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HEU5467058.1"
                     /translation="MADGLINLTIDDVAVSVPKGTLLIEAAKQAGVLVPHYCYHPGLP
                     VAGVCRMCLVEVEKAPKLQIACATQVAEGMVVRTRSGQAKSGRTGVLELLLINHPLDC
                     PICDQAGECELQDFTFQEGRPSTRYSPTYPKRFNPVEDFGPDVLYVPNRCILCTRCVR
                     FMEDVAKEPVLNVSERGDRAFVGIHPDAQLDHPWSGNVVDLCPVGSLLSKDFLHKARA
                     WELDKTASVCTGCSQGCNVTLDLRDDTVVRIRPRANLEVNQYFICDHGRGHYRWMNRG
                     DRVEAPLVREGADLRAVDWDEALDRVRTLLKGAGGSAVALVSPKASTEGLYFARKVLA
                     RFNWTGAFQVVMGEEAPLAGVPNLALRAERAPNGKGAELLGYTRDYAAALKAAEKAAV
                     VLVIDDPEAQIKTSGQVIYLGTAAPAFLNAAIVLPMALMVEEDGTYVNRDGRVQRYFQ
                     ARGTPAMARPAWWILGQLTDAAPESASAAFDAMAAEVGAFRGMSYATLGLLGQRSGAG
                     AGVPA"
     gene            complement(1879..3159)
                     /gene="nuoF"
                     /locus_tag="VFU75_09215"
     CDS             complement(1879..3159)
                     /gene="nuoF"
                     /locus_tag="VFU75_09215"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HEU5467059.1"
                     /translation="MGYPQKPHPKETVVLSEYFGDPEARTFKGWVKRGGYEALKKALA
                     MDPNAVTDEVKNSGLRGRGGAGFPTGLKWTFMPKEKRGPHYLCVNADESEPGTFKDRE
                     IMRWTPHALLEGTAIAAHAIRAEVAYIYIRGEFTEPWTVMTQALAEANAAKVFGNITI
                     HLHRGAGAYICGEETALMNSIEGKRGNPRIKPPFPAVAGVFGKPTTINNVETLAAVPH
                     IIKRGSAWYKGLCLSSPKSTGTKLFSVCGHIQKPGNVEVTMGFPLKDVIYEMCGGMRP
                     GRTLKACIPGGSSVPILTKDETEKCLLDYEGVAEAGSLLGSGGVIVFDDSADMVYQIM
                     RLARFYAHESCAQCTQCREGTAWTTKIMERILAGKGQMKDLDLLLDLSENMTGKTICV
                     LSDSCATPVISGIKKFRSEFEDYITGKKRPVMVA"
     gene            complement(3253..3762)
                     /locus_tag="VFU75_09220"
     CDS             complement(3253..3762)
                     /locus_tag="VFU75_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HEU5467060.1"
                     /translation="MSAAHAAEAEPYQPVFVGKTLDRLNEIIARYPAKQGALLPALWL
                     VQEARGWISDDAMAEVAAVLDTTAAHVKGVVTFYTMYHQHPVGKYFVQVCTTSCCNIC
                     GAEDVVKALLQETGAKELGATSPDGKWTVIEVECLGACGFPTPILVNDDFIENVTPDK
                     VPDLVRKYR"
     gene            complement(3759..5036)
                     /gene="nuoD"
                     /locus_tag="VFU75_09225"
     CDS             complement(3759..5036)
                     /gene="nuoD"
                     /locus_tag="VFU75_09225"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HEU5467061.1"
                     /translation="MSKRTVEISVATAGTDAQGNPLRVPIPASPADVDPELGGEHLLV
                     NLGPQHPATHGVLRLVVELEGETVVRVIPHVGYLHSGFEKLGEYRHYNQIVPLTDRTD
                     YLAPMSNNVGVVLAAEKLMGIEITPRCQVLRVIACEMSRIISHMVWLGTTSIDLGAFT
                     PFLWAFQQRERIYNLQEMWTGARLTTSLTRVGGLLADVPDKFVTDLRTFTETFPDTLD
                     EIDRVLTRNAVWVGRTQNIGVLTAEEAINYSLSGPMLRASGVPWDLRKDKPYLGYENY
                     DFDVPVGEHGDVYDRFLVRLEECYQSNKILKQALERLPDGPINVDDPRVILPPKSRAM
                     NDMEAMIYHFKQVMEGVKAPVGEAYAGIENPKGELGNYFVSDGTAKPVRWRIRPPAFI
                     NLASISKMAEGHLLSDLIAINASVDIVMGEVDR"
     gene            complement(5033..5650)
                     /locus_tag="VFU75_09230"
     CDS             complement(5033..5650)
                     /locus_tag="VFU75_09230"
                     /inference="COORDINATES: protein motif:HMM:NF012549.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HEU5467062.1"
                     /translation="MNASANALQATFGNAIGRVIDARGETIVYVSPAKVRDVLLWLKH
                     TAGQDYNYLVDVTAVEYRDRERPLEVVWNLRSLSTKQDLRIKVELDPRGELAVPSVVD
                     LWAGANWLEREVYDMFGVTFTGHPDLRRILMWETYAEGYPLRKDFPLRGRFSRSEQVR
                     QALNVNPEGHYSMEELAIADAYDELPRDMRDRLARGERDKIPNSE"
     gene            complement(5647..6279)
                     /locus_tag="VFU75_09235"
     CDS             complement(5647..6279)
                     /locus_tag="VFU75_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HEU5467063.1"
                     /translation="MKHLQTVDGSDSWLTTKAQSLFEWVIKTTRLDKAVNLSRANSLW
                     PMPFGTACCAIEFMATAASRFDLARFGMERQAFSPRQSDVLICAGRLPFKLAPVIRRI
                     YDQMPQPKWVISMGACASTGGIFDNYAMVQGIDTVVPVDVYVPGCPPRPEGLLYGILM
                     LHKKIKGEDLFDSSLRDEQLLNDKGLRLAPEQIDEISEPFGNSVHQFRSA"
     gene            complement(6287..6664)
                     /locus_tag="VFU75_09240"
     CDS             complement(6287..6664)
                     /locus_tag="VFU75_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HEU5467064.1"
                     /translation="MLQPYIPFLILLAFVIVNAVGMLVLSHFVGPRRPTPVKLAPYES
                     GMPPLGSAHQRFSVKFYLVAMLFIVFDIETVFLIPWATIFFGSGATGGPGMGFLLIEM
                     LVFLVILFVGYIYVWKRGAFQWD"
     gene            6777..7337
                     /gene="dcd"
                     /locus_tag="VFU75_09245"
     CDS             6777..7337
                     /gene="dcd"
                     /locus_tag="VFU75_09245"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HEU5467065.1"
                     /translation="MSIKSDRWIRRMAMERGMIEPFTDEQVREGVISYGVSSYGYDMR
                     VARDFKIFTNALSAIVDPKGFDPKSFVEYTGDVCVVPPNSFALARSVEYFRIPREILT
                     VCVGKSTYARCGIITNVTPFEPEWEGHVTLEISNTTPLPAKIYANEGIAQVLFFESDK
                     DDVCERSYKDKKGKYQAQRGITLPKI"
     gene            complement(7414..7836)
                     /locus_tag="VFU75_09250"
     CDS             complement(7414..7836)
                     /locus_tag="VFU75_09250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467066.1"
                     /translation="MADANDQSRVVVWFAVWATVCAAVAGIVVLLFDTYVFSHLVHRA
                     AIIHNLVDEGSGVLAISAGQGALALVTASALMQLGRALQPTALLGLLIGVFDFGFDLL
                     QVFVPSTEVEWKWKLVIALLAAVVITVSGSRKQAGVEK"
     gene            complement(7887..>9865)
                     /locus_tag="VFU75_09255"
     CDS             complement(7887..>9865)
                     /locus_tag="VFU75_09255"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5467067.1"
                     /translation="IASPDTQSARLWTKALSKVPHMSGTPQQAVTRDYVLNLMKSWGL
                     ETWFTTYDVFLPQPDSSALWLIAAPGAAPQPLSLAEPAVPGDSTSMGPQVLAFNGTAA
                     SGNVTGDVVYVGYGLIEDYKTLDSLGISVKGKIVIARYGRSYRGIKAREAERHGALGL
                     VLYSDPAEDGYVRGDVYPKGAMRPWGGIQRGSVLNPDGDPTTPGYASLPGARRVPFDS
                     LDVPHIPVIPIGYGNAERILNLLTGPSVPQSWQGGLPFRYHVGPGPAQVHLVLRVEQG
                     AHAMHPIWDTFGMIRGATYPDQWIVMGAHRDAWGPGADDNITGTTTVLATARAFAELA
                     KKGIRPARTIIFATWDAEEWGDVGSTEWVEQMADSLQQHAVAYINEDAMATGTNFGGA
                     GSPSLKPLIRASTRVVPALLGPGTLYDSWLAAHHGDTTALEIGNMGGGSDFAGFYHHL
                     GIPAGEIGFGGPGGVYHSMYDDYEWMTAFGDPQYRAHRTAAQLDAVIISRLANAAIAP
                     FDYAAFGTEMRELISELDTGIARKGWSVSTEAAKTALDRFIVSARAFAAARDSGLAGN
                     LSSAKAAAATRALMQVERRLTRPQGLTYAGSWFKSLQFASDVDNGYATLAFPTVAEAI
                     RYGDQAVTAREIADLASRIDAARSALEDARAALR"
BASE COUNT         1699 a         3202 c         3255 g         1709 t
ORIGIN      
        1 ccccacttgg tggcgagcgg cgcggcgaac ggaatgacgg cgaaggtcag gagcgccggc
       61 gtaaagctga tcgcgggtgc gaggatgaag agccacttgt cggcgcgcgc cgggtacgtc
      121 tcttccttca cgatgttctt caggccgtcg gcggccggct gcagcagtcc gaagggcccg
      181 acccggttgg ggccgagccg gtcctgcatg aacgcgcaga gacggcgttc gaccagcgtc
      241 atgaacgcca cgccgaccat gatgaccgtg aacacgacga tcatcttgat gagcgacgtg
      301 acgatgaaga acagggtcgg actcacgcgg ggacccccgc tccggcgccg ctccgctggc
      361 ccaggaggcc gagtgtggcg tacgacatcc cgcggaaggc cccgacttcg gcggccatcg
      421 cgtcgaatgc cgcggacgcc gactccggag ccgcatcggt cagctggccg aggatccacc
      481 aggccggacg ggccatggcg ggtgtgcccc gggcctggaa gtaccgttgc acgcgtccgt
      541 cgcgattcac gtacgtcccg tcttcttcca ccatcaacgc catcggcaga acgatcgcgg
      601 cgttcaggaa cgccggcgcg gcggtgccaa ggtagatcac ctggccggag gtcttgatct
      661 gcgcttcggg atcgtcgatc acgaggacca cggcggcctt ctcggcggcc ttcagcgcgg
      721 cggcgtaatc gcgggtgtac cccaggagct cggccccctt cccgttcggc gcccgctccg
      781 cgcgaagcgc gagattggga acaccggcga ggggagcttc ttcgcccatg acgacctgga
      841 acgcgcccgt ccagttgaac cgggccagca cctttcgggc gaagtagaga ccttcggtgg
      901 aggctttggg ggataccagg gcaacggcgg agccgcccgc gcccttgagc agcgtgcgca
      961 cgcgatcgag cgcctcgtcc cagtccacgg cacgcaggtc ggcgccctct cgcaccagcg
     1021 gcgcctcgac gcggtcaccc cggttcatcc accggtagtg cccgcggcca tgatcgcaga
     1081 tgaagtactg gttgacctcg aggttcgcgc gcgggcggat gcgcaccacc gtgtcgtccc
     1141 ggaggtcgag ggtcacgttg catccctgcg agcagccggt gcagaccgag gcggtcttgt
     1201 cgagctccca ggcgcgggcc ttgtgcagga aatccttcga caggagcgaa ccgaccgggc
     1261 agaggtccac cacgtttccg gaccacgggt ggtcgagctg ggcgtccgga tggatgccga
     1321 cgaacgcccg gtcgccgcgc tcggagacgt tcagcacggg ctccttcgcg acgtcctcca
     1381 tgaaccggac gcaccgggtg cacaggatgc agcggttggg cacgtagagg acgtcgggcc
     1441 cgaagtcctc caccggattg aagcgcttcg gataggtcgg cgaatagcgc gtggacggcc
     1501 ggccttcctg gaaggtgaaa tcctgcaatt cgcactcgcc cgcctgatcg cagatcgggc
     1561 aatcgagcgg gtggttgatg aggagcagct cgagcacgcc ggtgcggccg ctcttcgcct
     1621 gccccgaacg cgtgcgaacg accatccctt cggccacctg cgtcgcgcag gcgatctgga
     1681 gcttcggtgc tttctcgacc tccaccaggc acatccggca cacgccggcg accgggagac
     1741 ccgggtgata gcagtagtgg ggcaccagga cgccggcttg cttcgcggcc tcgatcagca
     1801 gcgtcccctt gggaacgctg accgccacgt cgtcgatcgt caggttgatc aagccgtcag
     1861 ccatcagccg tcagcctttc aggcgaccat cacgggtcgt ttcttgcccg tgatgtagtc
     1921 ttcgaattcc gaacggaact tcttgatacc ggagatcacc ggtgtcgcgc agctgtcgga
     1981 cagcacgcag atcgtcttgc ccgtcatgtt ctcggagaga tcgagcagca ggtccagatc
     2041 tttcatctgg cctttgcccg ccagaattcg ctccatgatc ttcgtcgtcc acgccgtacc
     2101 ctcacggcat tgcgtgcact gcgcgcagga ttcatgagcg tagaaacgcg ccaaccgcat
     2161 gatctggtag accatgtcgg cggagtcatc gaacacgatc acgccacccg atcccagcaa
     2221 cgagcccgcc tccgccacgc cctcatagtc caggaggcac ttctcggtct cgtccttggt
     2281 gaggatcggc accgaggagc cgccggggat gcaggccttg agcgtccggc ccgggcgcat
     2341 gcccccgcac atctcgtaga tcacgtcctt gagcgggaag cccatcgtga cttccacgtt
     2401 cccgggcttc tggatgtgcc cgcagaccga gaagagcttg gtaccggtgc tcttcggaga
     2461 tgacaggcag agccctttgt accaggccga gccgcgcttg atgatgtgcg ggacggccgc
     2521 gagggtctcg acgttgttga tcgtggtggg tttgccgaac acgcccgcca ccgccggaaa
     2581 gggcggcttg atgcgcgggt tcccgcgctt gccctcgatc gagttcatca gggcggtttc
     2641 ttcgccgcag atgtaggcgc ccgcgccgcg gtgcagatga atggtgatgt tgccgaatac
     2701 cttggccgcg ttcgcctcgg ccaacgcctg ggtcatcacg gtccacggct cggtgaactc
     2761 gccccggatg tagatgtagg cgacctcggc gcgaatggcg tgcgcggcga tggcggtgcc
     2821 ttcgaggagc gcgtggggcg tccaccgcat gatttcgcgg tctttgaacg tgcccggctc
     2881 ggactcgtcg gcgtttacgc agaggtagtg cggacccctc ttttccttgg gcatgaaggt
     2941 ccacttcaat cccgtcggga agcccgcccc gccccggccg cgcagtcccg agttcttcac
     3001 ctcgtcggtg acggcgttcg ggtccatcgc gagcgccttc ttcagcgcct cgtagccacc
     3061 gcgcttgacc caccccttga acgtcctggc ctcggggtct ccgaagtact cggagaggac
     3121 cacggtctct ttggggtggg gtttttgagg atagcccata tcaatgccca aagtcggaac
     3181 ggggaacgcg gagcgcggaa ccgacgcccg cgccaccggt tccgcattcc gacttccgaa
     3241 ttccgcgttc gatcaccggt acttcctcac caaatcgggg accttgtccg gggtcacgtt
     3301 ctcgatgaag tcgtcattga cgaggatcgg cgtcgggaag ccgcaggcac ccagacactc
     3361 cacctcgatc acggtccact tgccgtccgg gctggtggca cccagctcct tcgccccggt
     3421 ctcctgcaag agcgccttca cgacatcctc ggcgccgcag atgttgcagc acgacgtggt
     3481 gcagacctgc acgaagtatt tcccgacggg atgctggtgg tacatcgtgt agaaagtcac
     3541 gacgcccttc acgtgcgccg ccgtcgtatc gagcacggcg gccacctcgg ccatggcgtc
     3601 gtcggagatc cagccgcgcg cctcctgcac cagccacaag gccggcagga gggcgccctg
     3661 cttcgcgggg taccgtgcga tgatctcgtt cagccggtcc agcgtcttgc cgacgaagac
     3721 cggctggtac ggttcggctt ccgccgcgtg ggccgcgctc accggtccac ctctcccatg
     3781 acgatgtcca ccgacgcgtt gatcgcgatc aagtccgaga gcagatgccc ttcggccatt
     3841 ttggagatcg acgccaggtt gatgaaggcg ggaggacgga tccgccaacg caccggcttc
     3901 gccgtgccgt ccgacacgaa gtagttcccc agctcgccct tcggattctc gatgccagcg
     3961 tacgcctccc cgacgggcgc cttcacgcct tccatcacct gcttgaagtg atagatcatc
     4021 gcttccatgt cgttcatcgc gcgcgatttc ggggggagga tcacccgcgg gtcgtcgacg
     4081 ttgatcgggc cgtctggcag ccgctccagc gcctgtttca ggatcttgtt cgactggtag
     4141 cactcctcga gccggacgag gaaccggtcg tacacgtcgc catgctcgcc caccggaaca
     4201 tcgaaatcgt aattctcata gccgaggtac ggcttgtcct tccggaggtc ccagggcacg
     4261 cccgaggccc gcagcatcgg gccggacagc gagtagttga tcgcctcttc ggccgtcagg
     4321 acaccgatgt tctgcgtgcg gcccacccag acggcgttcc gcgtcaacac gcggtcgatt
     4381 tcgtccagcg tgtccgggaa cgtctcggtg aacgtccgga ggtcggtcac gaatttgtcg
     4441 ggaacgtcgg cgagcaggcc gccgacgcgc gtgaggctcg tggtgagccg ggcgcccgtc
     4501 cacatctcct gcaggttgta gatccgctcg cgctgctgga acgcccacag gaacggcgtg
     4561 aaggcgccga ggtcgatcga cgtggtgccg agccagacca tgtgcgagat gatgcggctc
     4621 atctcgcagg cgatgacgcg aagcacctgg caccggggcg tgatctcgat gcccatcagc
     4681 ttctccgccg ccaggacgac acccacgttg ttcgacatcg gggcgaggta gtcggtgcgg
     4741 tcggtgagcg gaacgatctg attgtagtgc cgatactcgc cgagcttttc gaagccggag
     4801 tggagatagc cgacgtgcgg gatgacgcgg acgacggttt cgccctccag ctccaccacc
     4861 agccggagca cgccgtgagt cgccgggtgc tgtggcccga ggttgacgag gaggtgctcc
     4921 cctccgagct ccggatccac gtccgccggt gaggcgggaa tcgggactcg cagcggattg
     4981 ccctgggcgt cggtgcccgc ggtcgcgacg gagatttcga cggtgcgctt gctcattcgg
     5041 agttcgggat cttgtcgcgc tccccgcggg ccagacggtc gcgcatgtcc cgggggagtt
     5101 cgtcgtaggc gtcggcgatg gccagctctt ccatcgagta gtggccttcg ggattcacgt
     5161 tgagggcctg ccggacctgc tcggaccggg agaacctgcc gcggagcggg aaatccttcc
     5221 ggagggggta cccttcggcg taggtctccc acatcaggat gcggcggagg tcggggtgac
     5281 cggtgaaggt cacgccgaac atgtcgtaca cctcgcgctc gagccaattg gcgccggccc
     5341 acaggtcgac gacggacggg acggccagct cgccccgcgg atcgagctcg accttgatcc
     5401 ggagatcctg cttggtcgac agggagcgga ggttccagac cacctcgagc ggccgttcgc
     5461 ggtcgcggta ttcaacggcc gtcacgtcca ccaggtagtt gtagtcctgg ccggcggtgt
     5521 gcttcagcca gaggaggacg tcgcggacct tggccgggga gacgtacacg atcgtttcgc
     5581 cgcgcgcgtc tatcacgcgg ccgatcgcgt taccgaaggt cgcctgcagt gcgttcgcgg
     5641 aggcgttcac gccgatcgga actggtggac ggaattgccg aagggctccg agatctcgtc
     5701 gatctgctcg ggcgcgagac ggagaccctt gtcgttgagg agctgctcgt cgcggagcga
     5761 cgaatcgaag aggtcttctc ccttgatctt cttgtggagc atgagaatgc cgtacagcag
     5821 gccttccgga cggggtgggc acccgggcac gtagacgtcg accggaacca cggtgtcgat
     5881 cccctgcacc atcgcgtagt tgtcgaagat cccgccggtc gatgcgcacg cgcccatcga
     5941 aatcacccac ttgggctggg gcatctgatc gtagatgcgg cggatgacgg gcgccagctt
     6001 gaagggcagc cggccggcgc agatcagtac gtcggactgt cgcggcgaga acgcctggcg
     6061 ctccatgccg aagcgggcca ggtcgaaccg gctcgcggcg gtggccatga actcgatggc
     6121 gcagcacgcc gtgccgaagg gcatgggcca gagcgaattg gcgcgggaca gattgacggc
     6181 cttgtcgagc cgggtggtct tgatcaccca ctcaaacaga ctctgagcct tggtcgtcag
     6241 ccagctgtcc gagccatcga cggtttgcaa gtgcttcata gatctctcaa tcccactgaa
     6301 aggcgcctcg cttccagacg tagatgtagc ccacgaagag gatcacgagg aacaccagca
     6361 tttcgatgag caggaacccc atcccgggtc cgccggtggc gcctgagccg aagaagatgg
     6421 tcgcccaggg gatgaggaag acggtctcga tgtcgaacac gatgaacagc atcgcgacca
     6481 ggtagaactt gacggaaaac cgctggtgcg ccgaccccag gggaggcatt ccgctctcgt
     6541 agggcgccag tttcacgggc gtgggacgcc ggggtcccac gaagtgggag agaacgagca
     6601 tcccaaccgc gttcacgatc acgaacgcca ggagtatgag gaacgggata tagggctgga
     6661 gcattagtct cgtgaaataa ttcacgtgtt ggatttcgga agctatcggt cgtcggggtc
     6721 actgtcaacc gaaacgagtg gcgtaagtct tgatggcgcc gatatattgc ccgcctatgt
     6781 cgatcaaatc agaccgctgg attcggcgga tggcgatgga gcgcggcatg atcgagccct
     6841 ttacggacga gcaggtccgg gagggcgtga tttcctacgg cgtgtcgtcc tacggctacg
     6901 acatgcgggt ggcgcgggac ttcaagattt tcacgaacgc cctgtcggcc atcgtggacc
     6961 ccaagggttt cgatccgaaa tccttcgtcg agtacaccgg ggacgtctgc gtcgtcccgc
     7021 cgaactcctt tgccctggcg cgctccgtcg agtacttccg gattccccgg gagattctga
     7081 ccgtctgcgt gggcaagagc acctacgccc gctgtggcat cattaccaac gtcacgccct
     7141 ttgagcccga gtgggaaggc cacgtgacac tggagatctc gaacaccact cccctgcccg
     7201 ccaagatcta cgccaacgag ggcatcgcgc aggtgctgtt cttcgagtcg gacaaggatg
     7261 acgtttgcga gcggagctac aaggacaaga aggggaagta tcaggcgcag agagggatta
     7321 cactgccgaa aatctgaagg cgtcaaggaa aggcgtcgag gaaatacgtc aaggagaggc
     7381 gtaaacgact tacctcgacg attctcctcg acgctatttc tcgacgcctg cctgctttcg
     7441 agatcccgat acggtgatga caaccgccgc caacaacgcg atcaccagct tccacttcca
     7501 ctcgacctcg gtggacggga cgaacacctg caggaggtcg aacccgaagt cgaacacgcc
     7561 gatgagcagg ccgaggagtg cggtgggctg aagggcacgc ccgagctgca tgagcgcgct
     7621 ggcggtcacg agggcgagcg ccccctgccc cgccgaaatg gccaggactc cgctgccctc
     7681 atccaccagg ttgtggatga tcgccgcccg gtggaccagg tggctgaaga cgtaggtgtc
     7741 gaacagcagt acgacgatcc ccgccaccgc ggcgcagacg gtcgcccata cggcaaacca
     7801 caccaccacc cgggactgat cgttcgcgtc ggccatgctc cactccgtcg aggacggccc
     7861 aatctgtgac ccggcccgcc ttcgcgctag cgcaatgccg cgcgggcgtc ctccagggcg
     7921 gaacgagcag cgtcaatgcg ggaggccaga tccgcgatct cccgcgccgt cacggcctga
     7981 tccccgtacc ggatcgcctc ggccaccgtg gggaacgcga gggtcgcgta gccgttgtcc
     8041 acgtccgagg cgaattgcag gctcttgaac cacgagcccg cgtacgtcag tccctgcggc
     8101 ctcgtgaggc ggcgctcgac ctgcatcagc gcccgcgtcg cggccgcagc cttggccgag
     8161 gacaggtttc cggcgagtcc ggaatcccgc gctgccgcaa aggcacgggc gctgacgatg
     8221 aaccggtcga gggcggtctt cgccgcctca gtgctcaccg accagccctt gcgggcaatc
     8281 ccggtgtcga gctcggagat gagctcgcgc atctcggttc cgaaggccgc atagtcgaag
     8341 ggtgcgatcg cggcgttggc gagacgcgag atgatcaccg cgtcgagctg cgccgcggtc
     8401 cggtgcgccc ggtattgcgg atcgccgaag gcggtcatcc actcgtagtc gtcgtacatc
     8461 gagtgataga cccccccggg gccgccgaat ccgatctccc ccgcgggaat cccgagatgg
     8521 tggtagaacc cggcgaaatc cgagccccca cccatgttcc cgatctcgag ggcggtggtg
     8581 tccccatggt gcgccgccag ccaggagtca tacagggtcc cgggacccag gagcgccggc
     8641 accacgcggg tggaagcgcg gatcagcggc ttgagtgagg gcgagccggc gccgccgaag
     8701 ttcgtgcccg tcgccatggc gtcttcattg atgtaggcga cggcgtgttg ctgcagcgaa
     8761 tccgccatct gctccaccca ctccgtcgac cccacatccc cccactcctc ggcgtcccag
     8821 gtcgcgaaga tgatcgtgcg cgccggacgg atgcccttct tcgccagctc cgcgaacgcg
     8881 cgggccgtcg cgagcaccgt cgtcgtgccg gtgatgttgt catcggcccc cggtccccag
     8941 gcatcccggt gcgcgcccat cacgatccat tgatcggggt aggtcgcgcc ccggatcatc
     9001 ccgaaggtgt cccagatcgg atgcatggcg tgtgcgccct gctccacgcg gagcaccaga
     9061 tgcacctgtg cgggtccggg cccgacgtga taccggaacg gcagtccgcc ctgccacgac
     9121 tgcggcaccg agggtccggt cagcaggttg agaattctct cggcgttgcc gtatccgatg
     9181 gggatgacgg ggatgtgggg cacgtcgagc gaatcgaatg gcacgcgtcg ggcaccgggg
     9241 agggacgcgt accccggtgt cgtcgggtcg ccatcgggat tgagcacgct gccccgctga
     9301 atgccgcccc aggggcgcat cgcgcccttg ggatacacgt cgccccggac gtatccgtcc
     9361 tcggccggat ccgaatacag aaccagaccg agggcgccat gacgctcggc ttcgcgggcc
     9421 ttgatcccgc ggtaggaccg tccgtaccgg gcgatcacga tcttgccttt gacgctgatg
     9481 cccagcgagt cgagggtctt gtagtcctcg atcaagccat acccgacgta gacgacgtcg
     9541 ccggtgacgt tgccgctcgc cgccgttccg ttgaacgcga ggacctgcgg ccccatggag
     9601 gtggagtccc cgggaacggc gggctccgcc aatgagaggg gttgcggtgc ggcgccaggc
     9661 gcggcgatca gccagagcgc cgacgagtcg ggttgcggca ggaagacgtc gtaggtggtg
     9721 aaccaggttt cgagacccca cgacttcatg agattcagca cgtagtcccg cgtcacggcc
     9781 tgctggggcg ttcccgacat gtgcgggacc ttgctcagtg ccttcgtcca cagccgcgcc
     9841 gactgggtgt cgggactggc gatca
//
LOCUS       DASUBP010000244         3357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_621620, whole genome shotgun sequence.
ACCESSION   DASUBP010000244 DASUBP010000000
VERSION     DASUBP010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621620"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(124..2880)
                     /gene="acnA"
                     /locus_tag="VFU75_09260"
     CDS             complement(124..2880)
                     /gene="acnA"
                     /locus_tag="VFU75_09260"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HEU5467068.1"
                     /translation="MSQTNTFGSHTTLAVGGKRFTIHKLETLEKKGLPVSRLPYSLKI
                     LLENLLRLEDGRTVRAQDVEKLTKWDPGHIPDEEIAFMPARVLLQDFTGVPAVVDLAA
                     MRDAMSRMGGDPKKINPLLPAELVIDHSVIVDQFGTPTAFQANAELEFQRNRERYALL
                     RWAQKTFRGFRVVPPDTGIVHQVNLEYLARVVFTGDGNEPLAYPDTVVGTDSHTTMIN
                     GLGVLGWGVGGIEAEAAMLGQPISMLIPQVVGFRLSGRLREGTTATDLVLTVTEMLRK
                     KGVVGKFVEFYGPGVANLPLADRATIGNMAPEYGATCGIFPVDAETLKYLEFTGRPKD
                     QVALVEAYTKEQGLFLTPDSPEPKFSDTLSLDLATVEPSLAGPRRPQDRVALHDVAKG
                     FAAALPSLIKPSAAPAPTGRWEGEGGGTAVATPPARKTHVDLTINGEACQLRHGSVVI
                     AAITSCTNTSNPSVMVAAGLLAKKAVEKGLMSKAWVKTSLAPGSKVVTDYLGQAGLTP
                     FLDKLRFNLVGYGCTTCIGNSGPLPEEVTAAITEGELVTCAVLSGNRNFEGRIQQEVR
                     ANYLASPPLVVAFALAGRIDFDFEKDALGKGKDGLPVYLKDIWPSAQEVDSVVRQSVQ
                     SSMFHKEYSEVFAGDARWQGLPVPDGERFVWEKDSTYIRQAPYFDGMPAQPGPISDIK
                     NARVLAVLADSITTDHISPAGSIRKTSPAGQYLISHGVQPADFNSYGSRRGNHEVMVR
                     GTFANVRLKNKLAPETEGPFTVHLPDGAPMSIYDASLNYQAEKTPLLVLAGKEYGSGS
                     SRDWAAKGPRLLGVRVVIAQSYERIHRSNLVGMGVLPLQFAAGESAESLGLTGKETFD
                     FVGVTDVLVEGFPKGKTLTVTAKGESGTKSFKVTVRIDTPQELQYYRHGGILEYVLRQ
                     LAAK"
     gene            complement(2877..3248)
                     /locus_tag="VFU75_09265"
     CDS             complement(2877..3248)
                     /locus_tag="VFU75_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467069.1"
                     /translation="MTLSRKAKLIISGIVALPILLFVFFTWSSLSWTYSSGTRAGYVQ
                     KFSKQGWLCKTWEGELSMVAVPGSTPEKFYFTVRNDSVAGLVNNTLGRRVALHYEQHK
                     GVPTSCFGLTEYYVDSVRTVE"
BASE COUNT          559 a         1109 c         1087 g          602 t
ORIGIN      
        1 ggctggccgg gatgtcttga cggcatccgc gacctcgcgt ccggtaaggg acaataggcc
       61 gcgtgacgtc actcttcgca ccaccttcgc gcccaatcgc cgggcgcccg cgacgaacgg
      121 cggctacttc gccgccagct gtctcagcac gtactcgaga atcccgccgt gccggtagta
      181 ctgcagctcc tgcggcgtgt cgatgcgcac ggtcaccttg aatgatttgg tgccgctctc
      241 gcccttggcg gtcacggtca gcgtcttgcc cttcgggaac ccctcgacca ggacgtcggt
      301 cacgcccacg aaatcgaacg tctccttgcc ggtgagcccc agagactccg ccgactcacc
      361 cgcggcgaac tgcagcggga gaacccccat tcccacgagg ttcgaccggt gaatgcgctc
      421 gtaactctgc gcgatcacca cgcggacgcc caggagacgc ggccccttgg cggcccagtc
      481 tcgtgacgaa cccgagccgt actccttgcc ggcgaggacc aggaggggcg tcttctccgc
      541 ttggtaattg agcgacgcgt cgtagatcga catcggcgcg ccgtcgggca gatgcaccgt
      601 gaagggcccc tcggtctccg gcgccagctt gttcttgagg cggacgttcg cgaacgtgcc
      661 gcgtaccatc acctcgtggt tgccccggcg cgagccatac gaattgaagt ccgcgggctg
      721 gacaccgtgg gaaatgagat actgcccggc gggactcgtc ttccgtatcg acccggcggg
      781 agagatgtgg tcggtcgtga tgctgtcggc caatacggcc agcacgcggg cgttcttgat
      841 gtccgagatc ggaccgggct gagcgggcat gccgtcgaaa tagggcgcct gccggatgta
      901 ggtcgaatcc ttttcccaca cgaagcgctc gccgtcgggg acgggaagac cctgccaacg
      961 cgcgtccccc gcgaacacct cgctgtactc cttgtgaaac atcgacgact gcaccgactg
     1021 ccgcaccacg ctgtccacct cctgcgcgct cggccagatg tccttcaggt agacgggcaa
     1081 gccgtctttc cccttgccca gcgcgtcctt ctcgaagtcg aagtcgatcc gcccggccag
     1141 cgcgaacgcg acgacgagcg gcggcgaggc gagatagttg gcgcggacct cctgctgaat
     1201 ccgcccctcg aagttccggt tccccgagag cacggcgcag gtcacgagct caccttcggt
     1261 gatcgccgcc gtgacttctt ccggcagggg cccggagttc ccgatgcacg tggtgcagcc
     1321 gtagcccacc aggttgaaac gaagcttgtc cagaaagggc gtcagccccg cctggccgag
     1381 gtagtcggtg acgaccttgg atccgggggc gaggctggtc ttcacccagg ccttgctcat
     1441 caagcccttt tcgacggcct tcttggccag caatcccgcc gccaccatca ccgaaggatt
     1501 cgacgtgttg gtgcagctcg tgatcgccgc gatgaccacc gagccatgac ggagctgaca
     1561 ggcctcgccg ttgatcgtga ggtcgacgtg cgtcttccgg gcgggcggag tcgcgaccgc
     1621 ggtcccgcca ccctcgcctt cccagcggcc ggtgggcgcc ggagccgcgg agggcttgat
     1681 gagcgagggc aaggcggccg caaatccctt ggcgacgtca tgcagggcca cccggtcttg
     1741 cggacggcgc ggtccggcga ggctcggttc tacggtcgcc agatcgaggg agagcgtgtc
     1801 cgagaacttg ggctccggtg agtcgggggt gaggaagagc ccctgttctt tcgtgtacgc
     1861 ctcgacgagg gccacctggt ctttcggccg tccggtgaac tcgaggtact tgagggtctc
     1921 ggcgtccacc gggaagatgc cgcacgtggc gccatactcg ggtgccatgt tgccgatcgt
     1981 cgcgcgatcg gcgaggggca ggttcgccac gccggggcca tagaactcca cgaatttccc
     2041 gacgaccccc ttcttccgaa gcatttcggt gaccgtcagc accaggtccg tcgccgtcgt
     2101 gccctcgcgc agccttccgg acagccggaa gcccacgacc tgaggaatca gcatggagat
     2161 gggctggccc agcatggcgg cttcggcctc gatgcccccc acgccccagc ccagcacgcc
     2221 gagtccgttg atcatggtgg tgtgcgagtc ggtaccgacg acggtgtccg ggtacgcgag
     2281 cggttcattg ccgtcaccgg tgaacacgac ccgagcgaga tactcgaggt tcacctggtg
     2341 gacgatcccg gtatcgggtg gtacgacccg gaatccccgg aaggtcttct gggcccagcg
     2401 cagcagcgcg tagcgctccc gattccgctg gaactcgagc tcggcgttgg cctggaacgc
     2461 cgtgggcgtc ccgaactgat ccacgatcac cgagtggtcg atcaccagct cagcgggcag
     2521 cagcggattg atcttcttgg ggtctccgcc catccgggac atcgcgtcgc gcatcgccgc
     2581 caggtccacc accgcgggga cgcccgtgaa gtcctgcagg agcacccggg ccggcatgaa
     2641 cgcgatctct tcatcgggga tgtgcccggg atcccacttg gtgagctttt cgacgtcctg
     2701 ggcgcggacg gtccggccat cttccaaacg gaggaggttc tcgaggagga tcttgagcga
     2761 gtaggggagc cgggagaccg gcaggccctt cttctcgagg gtctcgagct tgtgaatggt
     2821 gaaacgcttc ccgcccaccg cgagggtggt gtggcttccg aaggtattcg tctggctcat
     2881 tcgaccgtcc gtacgctgtc tacgtagtac tccgtgagtc cgaagcagct ggtgggaacg
     2941 cccttgtgtt gctcgtagtg caatgccacc cgcctgccca gggtgttgtt gaccagcccc
     3001 gcgaccgaat cgttcctgac cgtgaagtag aacttctcgg gcgtcgatcc gggcaccgcc
     3061 accatcgaca gctccccttc ccacgtctta cagagccacc cctgcttcga gaacttctgc
     3121 acataccccg cccgggtacc gctggagtag gtccagctca gggacgacca cgtgaagaac
     3181 acgaagagga gtatcggcaa cgcaacgatg ccggagatga tgagcttcgc cttgcgggag
     3241 agggtcattg ggtcaatcta cctcgtacgt ttcggtgcga taaggcacga agccgcgcgc
     3301 cttgtagttc ggcaccgcgt tggggtgatc gagcgtgcag gtgtgcagcc aaacgcg
//
LOCUS       DASUBP010000245        17728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_212312, whole genome shotgun sequence.
ACCESSION   DASUBP010000245 DASUBP010000000
VERSION     DASUBP010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 17728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17728
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212312"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="VFU75_09270"
     CDS             complement(<1..554)
                     /locus_tag="VFU75_09270"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEU5467070.1"
                     /translation="MFHGTRPGWIEVIAGVMFSGKSEELIRRVRRAVIARKKVQVFKS
                     HLDARYAGLYSVTSHDGITVEAEPIDSSEQLIRQIAPVTEVVAVDEAQFLDDGLVAVA
                     NSLADRGVRVILAGTDVDFRGLPFGAMPTLMCVAEVVDKFQAICVVCGGPATRNQRLV
                     NGRPATWDSPTIMVGGRESYEARCR"
     gene            801..1094
                     /locus_tag="VFU75_09275"
     CDS             801..1094
                     /locus_tag="VFU75_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003215863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEU5467071.1"
                     /translation="MNKAELTAALAMRTKMSKADAGRTIEALFDDKGIIAGELKKGAK
                     VQITGFGNFEARKRAARMGRNPKTGGVIQIKASIAPAFRAGKQLKMAVNGKMK"
     gene            complement(1256..1633)
                     /locus_tag="VFU75_09280"
     CDS             complement(1256..1633)
                     /locus_tag="VFU75_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467072.1"
                     /translation="MDPQREQGFELTGYSGNSTHGTVTVISFDKKARTRRAFKALGTF
                     WACAVGSIFIPVAHFLLVPGFFFFAIFQFFQRLGTPELGKGATGVCPDCGAEQKLDLP
                     PRWTTPQQVTCKKCQRGLQLTAA"
     gene            complement(1666..2892)
                     /locus_tag="VFU75_09285"
     CDS             complement(1666..2892)
                     /locus_tag="VFU75_09285"
                     /inference="COORDINATES: protein motif:HMM:NF015817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DbpA RNA binding domain-containing protein"
                     /protein_id="HEU5467073.1"
                     /translation="MAATPATAVLERGRPVLIVSPPAPECAGALWPLVQGHAPVLIIT
                     ESPSAAQAWLLAVPQPLRFHAVTGLDRTARLLASGEVDVLAGSPTDCAALLARSALKG
                     EEIRTVVLAWPEMLQGTPEFSAVPALLAEMKEARRIVLSWNPTGLESLLESHARRPHV
                     VGDMPIGENARPLPTLGEAKYVIAAPGTREAAITAALDGLDPGVALVWRRGVEPAGQA
                     NAVICADLPTRAELAALATVGMPVLVLAPEQLAYARSVASPLTPVPLLTGAGTAARQA
                     RATIAARVERGDLAAELALLAPLFERYDAAEVAAAVYAVSREAGSGKREAEGAALSRV
                     KIHVNVGKKDRAAAKDIVGALIREAGLSKDDIGRIDVRDTHSVVEVAGSAAERAVARL
                     SGMMIRGRRVNARIDR"
     gene            3023..4234
                     /locus_tag="VFU75_09290"
     CDS             3023..4234
                     /locus_tag="VFU75_09290"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEU5467074.1"
                     /translation="MADGLSQNLQHLETSATIAISQEAKRRRSAGEDVIDLGAGEPDF
                     ATPSIPAEAGIKAIKDGYTHYPANEGILELRAAAAKHLSLLSGGRPVNADHIVVSSGS
                     KQSLFNACFSLFGPGDSVAIPAPAWVSYPQIIHLARARPVMVAGDAEWGLKVGVKDLE
                     RMVPTPKGLILCSPCNPTGAVYSLTELRAIAEWAERKNAWIIADEIYRRIHYGPGPAP
                     SFLDLNDELLKRVVLITGPSKAYAMTGWRIGLALAPQPIAKAMAALQSHTTSGANHQA
                     QFAAAAAYADERVEQDVQRMVAEFRKRRDLVVGRFRQDLPGVEFVEPLGAFYFFFRVD
                     SFGGMHGTDFCTQLITETGVALVPGAAFGDDRWVRLSYAAATTDLERALDRILEFVTV
                     REKGSGKRDGV"
     gene            4224..4742
                     /locus_tag="VFU75_09295"
     CDS             4224..4742
                     /locus_tag="VFU75_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467075.1"
                     /translation="MVFEARIVPLDGKLPKVTYRWDAETDILTVSCKGTARNGKGLSG
                     TVDLEGADGSFVVLDVADGALRGLDVVTWPDDIKTLNTLTPPADPKEGAVIFPSRNSQ
                     PGVAAVEVDTALMMEKNAAESILHLKVGRARAATPVRVGDHLLVEIDKQNRLAGVWLL
                     DVPPFPNVEVTA"
     gene            4745..5377
                     /locus_tag="VFU75_09300"
     CDS             4745..5377
                     /locus_tag="VFU75_09300"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEU5467076.1"
                     /translation="MDERLERYLRELFFVEDAALVEIRKHQAASNLPEINVSAEEGRL
                     LAVLLAAVQATRVLEIGALGGYSGAWMARALPPSGKLTTIEFDPHHAKVAREAYRTAG
                     VADRVELIEGSALDVLPTLKPGFDAVFVDADKAPMAQYFEWGVRLLRVGGLLLGDNAF
                     HDGKVADPSVTAADTEGVRAFNKLSATDPRINATVIPIRDGLVVGVKVRD"
     gene            5377..6378
                     /locus_tag="VFU75_09305"
     CDS             5377..6378
                     /locus_tag="VFU75_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467077.1"
                     /translation="MARLAWLDAALAAGAVPASYVPDASGGRSEAEELTGADLVEGSQ
                     LAVRALGTEALAPSAVTFIDGIQRWAVVGYDGVVPIISAYVAAAARRRKGGRLQTVAE
                     RSRRVVITAVDGLSDNVRDALQRSGEELITLKDEVLGQPGRALTAAGIQVERLRDAVE
                     RAVAEPLAERLPADEWLVIDGILSNSAVLASHARTLGVIKSHGAQYFSGDDLKRALTL
                     PQGHRTSVFQPHRHGSQAVYSWYVRIWPWEGHDLLYGLLRLEARAHRDSIGLADALGS
                     WLLNERKPLSTPDARFDRLLYPIHDVETYLRSRAPQYLVADPGSRLPRTAGTAGTGS"
     gene            6375..8300
                     /locus_tag="VFU75_09310"
     CDS             6375..8300
                     /locus_tag="VFU75_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467078.1"
                     /translation="MSVGRVVATELKPATPHQFHFWTARDTTIGIGSIVRVEDPADAA
                     RVVFGVVTDAFAYSDLATPLHDVVSADGDPDRASTAPTIRQEIRLWTAAVLRQFPEDP
                     LQPVPLGRVLVASDADVASALRMDAYLTGAEPTGIPVGLYTSGGLEAPVYLDSDFLLG
                     PEAAHLNISGISGLATKTSAVEFLISSIFQHFPAHRGRVAAVCFNVKGPDLCFLDRPG
                     ELQEEDRRRYERLGVRPEPFKRVRYYAPFKPDGVNLNTLRTHPDLVGDVEPLVWGLRE
                     ALDFAEVLLNRDDIDAKADAFIDFLKDRVVGQPFDDGFGNQHQVETFADLDALFRAIL
                     RGVEDSGRGDMWRTHHVATIRKVRNRLSNISTRCKGLVTDDGAVGDLPWGAFEDRGVY
                     VIDVANVDPLGQDLVFARVVSKLRERLEHRNLGVDVVVVFVDELNKYASGDGPETHVK
                     RMLLDISERGRYLGLALFGAEQFRSQVHRRVVGNAGTQVFGRMDADELATPGYQVLSP
                     ATKIKLATLPIGELMIRHPHFTQPIFVRFPRPAVMRGRDGVEQFPPGEDLPFEDAIVR
                     QLMKIDRRLKPNVLKDLVASREQEDVRRALAAVRRTRPDDVLAFFRSALGSKVAADVP
                     RERRAVPPLSPISEEPY"
     gene            8384..9577
                     /locus_tag="VFU75_09315"
     CDS             8384..9577
                     /locus_tag="VFU75_09315"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEU5467079.1"
                     /translation="MKLAHLSDLHLGFRQYERLTPRGANQREADVATALKRAVDDLLQ
                     QEPDCVVIAGDVFNAVRPTNAAILTLFRELDRLRNALPATKVIMVAGNHDTPRSAETG
                     SILRLYEALGVEIVIDVPRRIMLPDLDAAVLAVPHSAVAQAERPALRPQPGVTHNILV
                     IHAEVDGIFAFDKGAVEYGSGVVTRAELGASHWDYVALGAYHVARELEPNMWYSGSLD
                     YVSHNPWGELVDEKRHKRPGKGYLLVDLPGAKVTFRAIEAPRRFLDLPPIDATDQTAA
                     ELDAAIAASAKKVKPSVDDMVIRLIVNNVSRGIAKDLDHEAIRALKTRALNFHLDLRR
                     PPPPEREVGVATPGGKSLTLPETVKDFLSRRPLDADLDREEFIRVGVEYVEKAGAGTR
                     EEGPR"
     gene            9577..12006
                     /locus_tag="VFU75_09320"
     CDS             9577..12006
                     /locus_tag="VFU75_09320"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEU5467080.1"
                     /translation="MELRRLRLVNFRQHADTEIEFGKGITGIVGPNGAGKTTLLEAIS
                     WAIYGSQAARGDKDSVRNLRAKARSSVKVELELALGRHELRIVRGLHSAELYQDGVLL
                     ANSLKEVTEKAQKALGMTHDEFFNTYFTGQKDLAVMGTLKREERHAFLSRVLGYERLD
                     LARVDVRETRNALNGELKGLEAGLVDRATLEKARQEAEARLKEARGAVKSAEHARSDA
                     EKRLGEEEPRWKEWQARRDRTLSLDGDRRIADKEVETARQEFQRLDRELAAARDARRQ
                     LDQLEPTLAPILALEREREQLEGLQKEEVARKGEEAQLKELKRTTERLEKRIGELLPA
                     EESLAAVEREVKLVAEELATAERLAEEARTAWVRERQYAETKRNELREQWKEVKQERD
                     EIVSLGPEGICPTCKRPLGPEYAQVLTVLDAQLTGIKDNGEFFAKRVEQLEAEPASVT
                     EADKTRDEIAGRSRAVSEHAGNLRAQEQERRRAVAERETAVKRSRDLEQRLATRATGY
                     DRERHEAVRAQLAALKPVLLEATTLKDRAGRGEILVKEAELAEKVLSAREARAKELTD
                     AVKALGFSDAEFDSARQRHDKALVQVRAAEVSLAQMRGELTGAEGALVEARKREAERG
                     EKEKEVADLRIRLRLHNELDRALGDLRTGLNAAMRPEIATLASSFLSDLTDGRYSDVD
                     LSEDYRVIVLDEELPKPVLSGGEEDLANLVLRLAISQMIAERAGQPLSLLVLDEIFGS
                     LDEGRRHHVLTLLRRLGDRFPQVILITHVEQIRDGLDRVIRVDYDSGKGTSVVRDDTA
                     TLGGADAGVAA"
     gene            11987..12955
                     /locus_tag="VFU75_09325"
     CDS             11987..12955
                     /locus_tag="VFU75_09325"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5467081.1"
                     /translation="MRAWLPEREVHGPERAGEQDIEGLNRLFAEAFTDRYRRDGLVGV
                     RVPRLNPAIWRYALIDAGEGAMLWRDEHGDIAAFNLAHLCGREGWMGPLAVRTDRQGG
                     GVGKSVVGAAADWLIDRGAHTVGLETMPRTVDNIGFYSGLGFRPGHLTVTLTLDTSTR
                     RLPAPALLSEQTSDRQHSGVTAIRGLVEELAPGYDCSREILLTSEMSLGDTVLVWGDA
                     GLDGFALWHSAPLAEGREGDEVRVLKLAARSPESFTAVVSAVEAAASRSGIRRVAIRS
                     QTAYADAYQSLIARGYQVRWTDLRMYLTGYPERSPAKGVLFSNWEI"
     gene            complement(12999..13286)
                     /locus_tag="VFU75_09330"
     CDS             complement(12999..13286)
                     /locus_tag="VFU75_09330"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEU5467082.1"
                     /translation="MSALREMLQREGKHTAWWSDVPGAKSGDEASHWPETRWVVSGYL
                     RVRVGAETYDLGPGDRLDLPADTPHTTEVIGLGTAIHLVGTKDAKLVSLSA"
     gene            complement(13393..13554)
                     /locus_tag="VFU75_09335"
     CDS             complement(13393..13554)
                     /locus_tag="VFU75_09335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467083.1"
                     /translation="MNITLIVAIASLIAWAVLLFVMHIGSGSVNLLYAIAAIAVARRI
                     MVGNPKFLS"
     gene            complement(13800..14999)
                     /locus_tag="VFU75_09340"
     CDS             complement(13800..14999)
                     /locus_tag="VFU75_09340"
                     /inference="COORDINATES: protein motif:HMM:NF013832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467084.1"
                     /translation="MRVLKLLRFVQHAGGAASSALSALWTFPSILLSAFLVAWGAEAA
                     QFLMSQGLALAILAWLQTLPEFAVEAVIAWEAGHDPARAHLAIANLTGAIRLLLGLGW
                     PMIYVVQAWYGRRQRTERLPAIRLNPEHATEVVGLIPGLLYFGFIYAKRTITWIDALV
                     LLALYGAYLLALLRHPPESGEHVEDAPAVAQWAYRQPGWRRPAAIGGLFLVGGALLFF
                     TAHPFLESMLALAGTLGVSQFVLVQWLAPFLSEFPEKLSAFAWARRVSHAPMALMNLV
                     SSNINQWTVLAPMIPLVYGYSHWRATGTWSAFGFDGEQELEILITLLQSTVAVLVLLT
                     LEFQWYEAAGIFVLWLVQFLVPHWRVEVAWGYGVWIAVLLTLYVAGRRRPVALRAAWQ
                     SVRRLDG"
     gene            15098..15643
                     /gene="folE"
                     /locus_tag="VFU75_09345"
     CDS             15098..15643
                     /gene="folE"
                     /locus_tag="VFU75_09345"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004912347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEU5467085.1"
                     /translation="MPFEQLISEMLTQLGEDPNREGLLRTPHRVAESMKFLTRGYGMS
                     VEAVINDALFEETHESMILVRDIEIYSLCEHHMLPFYGRAHVAYLPNGKIVGLSKIPR
                     VVDVFARRLQVQERLTDQVADALMRVLEPLGVGVVIEAYHLCMMMRGVEKQNSKTITS
                     ALRGAFRDDAKTREEFLGLVG"
     gene            15643..16356
                     /locus_tag="VFU75_09350"
     CDS             15643..16356
                     /locus_tag="VFU75_09350"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEU5467086.1"
                     /translation="MTALAGKVALVTGASRGIGLGVANELRAAGAHVVRLARSLSDGS
                     SERQTDFRCDVSDSSQVERVIGQVLRDLRAPDIVVNNAGIFFIKRLEGTTPADFAQAI
                     GINLTGPFLIARALLPHLVKRGSGDLVTIGSVSDHVALPGSAAYAASKFGLRGMHGSI
                     AAELAGTGVRATLVSPGPVDTEIWDPIDPDSRTGFTKRKDMMRAEDVAEAVLFAVTRP
                     ARTAVTEIRLLPTGYSARG"
     gene            16356..>17728
                     /locus_tag="VFU75_09355"
     CDS             16356..>17728
                     /locus_tag="VFU75_09355"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU5467087.1"
                     /translation="MKRESGVGGRLLPVIMGLLMVGPLAAQGVDEIPDSTGWGLWSTA
                     ILTLAHAPDSATWVGTYGHGIFVLRPHATAWEHISESDDTSKHSISWDFVHAFAFGPK
                     GEVWYGTVGNGWGLSLDNGKTWKNWQYRELGPEWQYVAPNGIATRGDTTYIATADGLK
                     ITGDDGRTWLVVTDSMGATTAKDSVIGRIRNQYLFAIIVGDGGNMWVSELTGIAHSPD
                     GGRTWHEGFVRPPCRGVGCANRARAFRGDPGKPLWVATEDLAYQFAPQFGGWKSLIPD
                     SLVARLPPDTAEFVADTVIPADPGDCGGGIRVFDGPCPEPHPRRGAPWAARPAGLRHT
                     WFRRPIALADQPYIDQTYRFGSTMGGNFQAHQGVEFNNPDGTPVHAIGDGVVIYAGKA
                     EAGANTVAIRHDRRFSLTPGHGPGVNPASQYVFSVYYHNSKLLVKTGDRVKAGDVVSL
                     VGNTGRAT"
BASE COUNT         3031 a         5753 c         6090 g         2854 t
ORIGIN      
        1 cggcatcgtg cctcgtatga ctcccgcccc ccgaccatga tcgtcggtga gtcccaggtc
       61 gccggcctcc cgttgaccag gcgctgattc cgggtagctg ggcccccgca gaccacgcag
      121 atcgcctgaa acttgtccac cacctcggcg acgcacatga gggtgggcat ggccccgaac
      181 gggaggccgc ggaagtcgac gtcggtcccg gccaggatga ccctgacccc ccgatcggca
      241 aggctattcg cgacggcgac caggccatcg tccaggaact gtgcctcgtc cacggccacc
      301 acttcggtga ccggggcgat ctggcggatc agctgttcgg aggagtcgat gggctcggcc
      361 tccacagtga tcccgtcatg ggaggtgacg gaatagaggc cggcgtaccg ggcgtccagg
      421 tgggacttga agacctggac cttcttccgg gcaatcacgg cgcggcgaac ccgccggatg
      481 agctcttctg acttgccgga aaacatgacc ccggcgatga cttcgatcca accggggcgg
      541 gtgccgtgga acaaagaagg gctcggcgtc aggggttagg ggtcaggggt tcgacgcggg
      601 aacgggaaca ctgatccctg atccctgacc cccgacaggg tagtcgtctt ggaaacggag
      661 gtcaaggaag tgctggtatc ttgcggtttt tcgagggggt accccatatt gtatcggttt
      721 ttcaagggtt tttgggatgc ccccccgtac aggggcgatt ttgcaaggct ttttcgacca
      781 cctccaccca ggagtgcgcc atgaacaaag ccgagctgac tgcggccctc gccatgcgga
      841 cgaagatgtc caaggcggat gccggccgga cgatcgaagc cctgttcgac gacaagggga
      901 tcatcgccgg tgagttgaag aaaggtgcga aggtgcagat cacgggcttc ggaaatttcg
      961 aagctcggaa gcgtgccgct cgcatgggcc gcaatccgaa gaccggcggc gtcattcaga
     1021 tcaaggcgtc gatcgctccc gcgttccgcg ccggcaagca gttgaagatg gccgtcaacg
     1081 ggaagatgaa gtaagcaacg ggtttcgcta tactgtgacg tcgctgtgag acgtcatggt
     1141 gaccaatagc gaagcggtat gcgggctgcc ccgtcgggca gcccgtcgct tttccagcca
     1201 cggagttctc gtcgtacccg tgctttagcg cccgtcgctt tctcgccctt tagggttagg
     1261 cagcggtgag ctgcaaaccc ctctgacact tcttgcaggt gacctgctgc ggagtcgtcc
     1321 accgcggcgg gaggtcgagc ttctgctcgg cgccgcagtc cgggcatacg cccgtcgcac
     1381 ctttgccgag ctcaggcgtg ccgaggcgtt ggaagaattg gaagatcgcg aagaagaaga
     1441 agccgggtac gagaagaaag tgtgcgacgg ggatgaagat cgagcccacg gcgcatgccc
     1501 aaaaggtccc cagcgccttg aaggctcgtc gtgtccgagc cttcttgtcg aacgaaatca
     1561 ccgtcaccgt gccgtgcgtc gaatttccgc tataaccggt gagttcgaat ccttgttctc
     1621 tttgcggatc catcgaaatc ccctaacggt ctagccgtct agttcttagc ggtctattcg
     1681 ggcattcacc ctccgaccgc ggatcatcat gccggacaag cgggccaccg cgcgttcggc
     1741 ggcagacccc gccacttcta ccacactgtg tgtgtcccgc acgtcgatgc ggccgatatc
     1801 gtccttggac aggcccgcct cacgaatcag ggctccgacg atatcctttg ccgctgcgcg
     1861 gtccttctta ccgacgttca cgtgaatctt gacccgggaa agtgcagcgc cttccgcttc
     1921 ccgcttcccg cttcccgctt cccgactcac cgcataaacc gcggccgcca cttccgccgc
     1981 atcgtatcgc tcgaagagcg gcgcgagcag cgccagctcc gcggcgagat ccccacgctc
     2041 gacccgcgcg gcgatcgtcg cccgtgcctg ccgggccgcc gtgccggcgc ccgttaatag
     2101 aggaacgggc gtgagcgggg acgcgaccga gcgtgcatag gccagctgtt cgggtgccag
     2161 cacgagtacc ggcatgccaa cggtagccag ggccgcgagc tcagcccgcg tcgggaggtc
     2221 cgcgcagatg accgcgttgg cctgtcccgc cggctccacc ccacggcgcc agacaagggc
     2281 cactcccgga tccagcccat cgagcgccgc ggtgatcgct gcttcccggg tgcccggggc
     2341 ggcaatcacg tacttggcct cgcccagagt cgggagcggg cgggcattct ccccgatcgg
     2401 catgtcgccg accacgtggg ggcgccgcgc gtgcgactcg agcaagctct cgagtccggt
     2461 cgggttccag ctcagtacga tccgccgtgc ttccttcatc tcggccagca atgcggggac
     2521 ggcggagaac tcgggggttc cctgaagcat ctcgggccac gccaggacca cggtgcggat
     2581 ctcttcgccc ttcagggctg accgggcgag cagggcggcg cagtcggtcg gggacccggc
     2641 aaggacgtcc acctcgcccg aagccagcag gcgagccgtt cggtccagtc cggtaacggc
     2701 gtggaatcga agaggttggg gcaccgcgag tagccaggcc tgagcggcac tcgggctttc
     2761 cgtgataatc agcacgggag cgtgcccctg gaccagcggc cacagggcgc cggcgcattc
     2821 gggggccggg ggagacacga tcagtacggg gcgcccgcgc tccagtacgg ccgtcgcggg
     2881 cgtcgctgcc acaggtgctg tcacacggtc ctcagttgtc aaagggcgtt caattctcta
     2941 tcacgcatcc tagttacggg gctcgaaggt ggcaaggagc gaggctagat tccaattcga
     3001 acttcacctg cctggagact cggtggctga cggcctgtcg caaaacctgc aacatctcga
     3061 aacatcggcg accatcgcca tttcgcagga agcgaagcgc cggcgctccg cgggtgaaga
     3121 cgtcatcgac ctgggggcgg gcgaacccga tttcgcgacg ccctcaattc cggccgaagc
     3181 cggcatcaag gccatcaagg acggctacac gcactatccg gccaacgagg gcatcctcga
     3241 gctgcgcgcc gccgcggcga agcatctctc gctcctgtcg ggcggccggc ccgtcaacgc
     3301 cgatcacatc gtcgtctcct cgggctccaa acagtcgctc ttcaacgcct gcttctcgct
     3361 cttcggtccc ggcgactcgg tcgccattcc cgccccggcc tgggtctcgt atccgcagat
     3421 catccatctc gcgcgagccc gccccgtcat ggtggccgga gacgccgagt gggggttgaa
     3481 ggtgggcgtg aaggatctgg agcggatggt gccgactccg aaggggctga ttctctgctc
     3541 cccctgcaac cccacgggcg ccgtgtactc cctgaccgag cttcgcgcga tcgccgagtg
     3601 ggcggagcgg aagaacgcgt ggatcatcgc ggacgagatc taccgccgga tccactacgg
     3661 acccggtccg gcgccctcgt tcctcgattt gaacgacgag ttgctgaagc gggtcgtcct
     3721 gatcaccggg cccagcaagg cctatgcgat gaccgggtgg cggatcggac tggccctcgc
     3781 gcctcagccc atcgcgaagg cgatggcggc cctgcaatcg cacacgacga gcggcgcgaa
     3841 tcatcaggcc cagttcgcgg ccgcggcggc gtacgcggac gaacgcgtgg agcaggatgt
     3901 ccagcggatg gtggccgagt tccggaagcg ccgtgacctg gtcgtgggcc gcttccggca
     3961 ggatttgccg ggcgtcgagt tcgtcgagcc gctcggtgcc ttctacttct tcttccgcgt
     4021 cgactcgttc ggcggaatgc atggcacgga cttctgtacc caactgatca cggagaccgg
     4081 ggtggcgctc gtgcccggcg cggcgttcgg cgacgaccgc tgggtgcggc tgtcgtacgc
     4141 ggcggccacg accgacctcg agagagccct ggaccgtatc ctggagttcg tcaccgttcg
     4201 ggaaaaggga agcgggaaac gtgatggtgt ttgaagccag gatcgtccca ctcgacggca
     4261 agctgccgaa ggtcacctac cgctgggacg cggagaccga catcctgacc gtctcctgca
     4321 agggcacggc caggaacggc aagggactct ccggcaccgt ggatctcgaa ggagccgacg
     4381 gctcgttcgt ggtgctcgac gtcgccgatg gggcgctgcg cggcctcgac gtcgtgacct
     4441 ggcccgacga tatcaagacg ctgaacaccc tcacgccccc ggccgacccg aaggaaggcg
     4501 cggtcatttt tccgtcgcgc aattcccagc cgggggtggc ggccgtcgag gtggacacgg
     4561 cgctgatgat ggagaagaac gccgcggaat cgattctcca tctgaaagtg ggacgggccc
     4621 gcgccgcgac tcccgtccgc gtcggcgacc acctcctggt cgagatcgac aagcaaaacc
     4681 gattggcggg cgtctggctc ctcgacgtcc cgccctttcc gaacgtcgag gtcaccgctt
     4741 agccgtggac gaacggctcg agcgatacct gcgcgagctg ttcttcgtcg aagacgccgc
     4801 gctggtcgag attcggaagc accaggcggc gtcgaatctc cccgagatca acgtctccgc
     4861 cgaggaaggg cgcctcctgg ccgtgctgct ggccgccgtt caggctacgc gcgtcctcga
     4921 gatcggcgcc ctgggcgggt actcgggcgc ctggatggcc cgggcactcc cgccgagcgg
     4981 caaactgacg acgattgaat tcgatcccca ccacgcgaaa gtcgcgcgcg aggcctaccg
     5041 caccgccggc gtggcggacc gggtcgagct gatcgagggc tcggcgctcg acgtactgcc
     5101 aacgctgaag ccgggcttcg acgcggtgtt cgtcgacgcg gacaaggccc ccatggcgca
     5161 gtacttcgag tggggcgtgc ggctgctgcg ggtgggtggc ctgctcctcg gggacaacgc
     5221 cttccatgac ggcaaggtcg ccgatccgtc ggtcaccgcc gcggacaccg agggtgtccg
     5281 ggcattcaac aagctttcgg ctaccgatcc ccgcatcaac gcgacggtga ttcccatccg
     5341 cgacggcctg gtggtgggcg tgaaggtgcg ggactagtgg cgcgcctcgc gtggctcgac
     5401 gccgcgctcg ccgcgggtgc cgtacccgct tcgtacgtgc ctgacgcctc gggcgggcgc
     5461 agcgaggccg aagagctgac gggcgccgac ctcgtggagg gctcccagct cgcggtccgt
     5521 gcgctcggaa ccgaggccct cgcaccgtcc gccgtgactt tcatcgacgg cattcagcgc
     5581 tgggcggtgg tcggatacga cggcgtcgtc ccgatcatca gtgcctatgt ggccgccgcg
     5641 gcgcggcgtc gaaagggtgg tcgcctccag acggtcgcgg agcgatcccg gcgggtcgtg
     5701 atcacggccg tggacggctt gagcgacaac gtccgcgacg cgctacagcg ctccggggag
     5761 gagctgatca ccctcaagga cgaggttctg ggtcagccgg ggcgcgccct cacggccgcg
     5821 gggattcaag tggaacggct ccgggatgcc gtggagcggg ccgttgcgga gcctctggcg
     5881 gagcggctgc cggcggatga atggctggtg atcgacggga tcctgtcgaa ctccgcggtc
     5941 ctggcgagcc acgcccgcac cctcggcgtg atcaagagtc acggagcgca gtacttcagc
     6001 ggcgacgact tgaagcgcgc gcttacgctg cctcagggac accgcacctc ggttttccag
     6061 ccccaccggc acgggtccca ggcggtctat tcgtggtacg tgcggatctg gccctgggag
     6121 gggcatgacc tcctgtacgg tctgctccgc ctcgaagccc gcgcgcaccg cgattccatc
     6181 ggcctcgccg acgcgctcgg ctcctggctc ctgaacgagc gcaaaccgct atccaccccg
     6241 gacgcgagat tcgatcgatt gctgtatcct atccatgacg tggagaccta tctccgcagc
     6301 cgggcccccc agtacctggt ggccgacccc ggctcgcgtc tgccccggac ggccgggacg
     6361 gccgggaccg gctcatgagc gtcggacgag tcgtcgcgac cgagctcaaa ccggccacgc
     6421 cgcaccaatt ccacttctgg acggcgcggg acacgactat cggcatcggc tcaatcgtcc
     6481 gggtcgaaga cccggccgat gccgcgcgcg tggtcttcgg ggtggtgacc gacgccttcg
     6541 cgtactccga tctggccacg ccgctccatg acgtcgtcag tgccgatggc gatcccgacc
     6601 gcgcctcaac ggcgccgaca atacgccaag aaatccggct gtggaccgcc gccgtcctgc
     6661 gccaattccc ggaagatccg ctccagcctg tcccgttggg ccgggtgctg gtcgcgagtg
     6721 atgcggacgt cgcctcggca ctccgcatgg acgcctacct cacgggggcg gagccgaccg
     6781 gaatccccgt cgggctctac acgtcggggg gactcgaagc cccggtctac ctggactccg
     6841 acttcctgct gggccccgaa gccgcgcacc tcaacatctc cgggatttcg gggctggcga
     6901 ccaagacgtc ggcggtcgag ttcctgatct cctccatctt ccagcacttt ccggcgcacc
     6961 gcggccgcgt cgccgcggtc tgcttcaacg tgaaaggtcc ggatctctgc ttcctggacc
     7021 gccccggcga gctccaggaa gaggaccgcc gccggtacga acgcctcggc gtccgtccgg
     7081 aacccttcaa gcgggtgcgc tactacgcac cgttcaagcc cgacggcgtc aacctcaaca
     7141 cgctgcgcac gcaccccgat ctggtggggg acgtcgagcc gctggtgtgg ggtttgcgcg
     7201 aagccctcga tttcgccgag gtcctcctca acagggacga catcgacgcg aaggccgacg
     7261 cgttcatcga tttcctgaaa gaccgggtcg tcgggcagcc cttcgacgac ggcttcggga
     7321 accagcatca ggtcgagacc ttcgcggacc tggacgcgct gttccgggcc atcctgcgtg
     7381 gggtggagga ctcggggcgc ggggacatgt ggcggaccca tcacgtcgcc accatccgga
     7441 aggtgcggaa ccggctctcg aacatctcga cgcgctgcaa aggcctcgtg accgatgacg
     7501 gggcggtggg cgatctcccc tggggcgcgt tcgaagatcg cggcgtctac gtcatcgacg
     7561 tcgccaacgt cgaccccctc ggccaggatc tggtcttcgc gcgggtggtc tccaagctcc
     7621 gcgagcgtct ggagcaccgg aacctcggcg tggacgtggt cgtggtgttc gtggacgagc
     7681 tgaacaagta cgcgtcgggg gatggaccgg agacccacgt gaagcggatg ctcctcgaca
     7741 tctccgagcg cggccggtac ctggggctcg cgttgttcgg ggcggagcag ttccggtcac
     7801 aggtacaccg gcgggtggtg gggaatgcgg ggacccaggt gttcgggcgg atggatgccg
     7861 acgagctggc gacccccggc taccaggtcc tgtcgccggc gaccaagatc aagctcgcga
     7921 ccttgccgat cggcgagctg atgatcaggc acccgcactt cacccagccg atcttcgtcc
     7981 ggttcccgag accggccgtg atgcggggcc gggacggcgt ggagcaattc ccgcccggtg
     8041 aagacctgcc gttcgaggac gccatcgtgc ggcagctcat gaaaatcgac cggcggctga
     8101 agccgaacgt gctcaaggac ctggttgcct cacgcgagca ggaggatgtc cgccgggccc
     8161 tggcggccgt gcgacgcacc cggccggacg acgtgctcgc gttcttccgg agcgcgttgg
     8221 ggagcaaggt cgccgctgat gtgccgcggg aacggcgggc ggtgccgccg ctgtcgccca
     8281 tttctgagga gccgtactga gcgggaggtc tcgcatgcga gccgtcgtcg tcttggcgtg
     8341 cacgatcctg cataacacgt ccgaccggag cctcattcct tagatgaagc tcgcccacct
     8401 ctccgacctt cacctggggt tccgccagta cgagcggctg acgcctcgcg gcgcgaacca
     8461 gcgcgaggcc gacgtggcga ccgcgctgaa gcgggcggtg gatgacctgc tccagcagga
     8521 gcccgactgc gtggtgatcg cgggagacgt gttcaacgcc gtgcgcccga ccaatgccgc
     8581 gatcttgacg ctcttccgcg agctcgaccg tctccgcaat gcgctgcccg cgaccaaggt
     8641 gatcatggtg gcgggaaacc atgacacgcc gcggtcggcc gaaacgggaa gcatcctcag
     8701 gctctacgag gcgctcggcg tcgagatcgt gatcgacgtc ccccggcgga tcatgctgcc
     8761 cgacctggac gccgcggtac tggcggtccc gcactccgcg gtcgcgcagg cggagcgtcc
     8821 cgccctccgc ccgcaacccg gcgtgacgca caacatcctg gtcattcacg ctgaggtcga
     8881 cgggattttt gccttcgaca agggcgcggt cgagtacggc agcggcgtcg tgactcgcgc
     8941 cgagctcggc gcctcgcact gggactacgt tgccctcggc gcctaccacg tggcgcgtga
     9001 gctcgagccg aacatgtggt actcggggag cctggactac gtcagccaca acccttgggg
     9061 cgagttggtg gacgagaaac gccacaagcg cccggggaag ggctacctgc tggtcgatct
     9121 ccccggtgcc aaagtgacct tccgggccat cgaggcgccc cggcgtttcc tggacctgcc
     9181 gccgatcgac gccaccgacc agaccgccgc ggagctggat gcggcgatcg ccgcgtcggc
     9241 gaagaaagtg aaaccgtccg tcgacgacat ggtgatccgg ctgatcgtca acaacgtgtc
     9301 ccgcgggatc gcgaaagatc tggaccacga ggcgatccgc gcattgaaga ccagggcgct
     9361 caacttccat ctcgacctgc ggcgcccccc gccacccgag cgcgaagtgg gggtcgccac
     9421 gccggggggg aagagcctca cgcttcccga aacggtgaag gattttctgt cccgccgccc
     9481 cctcgacgcg gacctggacc gcgaggaatt cattcgggtc ggtgtggagt acgtcgagaa
     9541 ggcgggcgct ggcacgcgcg aggaggggcc ccgctagtgg agctgcgccg cctccggctc
     9601 gtgaacttcc gccagcatgc cgataccgaa atcgagttcg ggaagggcat cacgggcatc
     9661 gtcggcccca atggggcggg caagaccacg ctcctcgagg ccatctcctg ggcgatttat
     9721 ggctcgcagg ccgcccgggg cgacaaggat tccgtgcgga atctgcgcgc gaaggcacgc
     9781 tcctccgtca aagtcgagct cgagctggcc ctcggccgcc acgagctgcg gatcgtgcga
     9841 gggctccact ccgcggagct gtatcaggat ggcgtgctgc tcgccaactc gttgaaggaa
     9901 gtgacggaga aggcgcagaa ggcattgggc atgacgcacg atgagttctt caatacgtac
     9961 ttcaccggcc agaaggacct ggcggtgatg gggacgctca agcgcgagga gcggcacgca
    10021 tttctctccc gcgtcctcgg ctacgagcgc ctggatctgg cgcgggtcga cgtccgcgag
    10081 acgcgcaacg ccttgaatgg agagctcaag ggcctggagg ccgggctggt ggaccgggcg
    10141 accctcgaaa aagcccgtca ggaggccgag gcccggctga aagaagcccg cggcgcggtg
    10201 aagagcgccg aacacgcgcg gtccgacgcc gaaaagcggc tcggcgaaga ggagcccagg
    10261 tggaaggaat ggcaggcccg gcgtgaccgg acgctttcgc tcgatggcga ccgccggata
    10321 gccgacaagg aggtcgagac cgcgcgccag gagttccagc gtctcgaccg tgagctggcc
    10381 gcggcccggg atgcccgccg gcagctggat caactcgagc ctactctggc gcccatcctc
    10441 gcgctggagc gggagcggga acagctcgag gggctgcaga aagaggaagt cgcgcggaag
    10501 ggtgaggaag cccagctcaa ggagctcaaa cggacgacgg agcgcctgga gaagcgcatc
    10561 ggcgagctcc tgcctgccga ggaatccctc gcggccgtcg agcgggaggt caagctggtg
    10621 gcggaagagc tggccaccgc cgagcgactg gccgaggaag cccgtacggc ctgggttcgc
    10681 gagcgccagt acgccgagac caagcggaac gagctccgcg agcagtggaa ggaagtgaag
    10741 caggagcgcg acgagatcgt gtccctcggc cccgagggga tctgcccgac ctgcaagcga
    10801 ccgttggggc cggagtacgc gcaggtgctc accgtcctcg acgcgcaact gaccggcatc
    10861 aaggacaacg gtgagttctt cgccaaacgc gtggagcagc tggaggccga gcccgccagc
    10921 gtcacggaag ccgacaagac ccgggatgag atcgccggcc ggagccgggc ggtgagtgaa
    10981 cacgcgggca acctgcgtgc ccaggaacag gagcggcgcc gggcggtggc ggagcgggag
    11041 acggcggtca aacggtcgcg ggatctcgag cagcggttgg ccacgcgagc caccggttac
    11101 gaccgggagc gtcatgaagc ggtgcgcgcg cagctggcgg cgctcaagcc ggtgctgctc
    11161 gaggcgacga cgctgaagga ccgcgccggc cggggcgaga tcctggtcaa ggaggcggag
    11221 cttgcggaga aggtgctctc cgcccgcgag gcccgcgcga aggagctgac ggatgccgtg
    11281 aaggcactgg gattctcgga cgcggagttc gacagtgccc gccagcgcca cgacaaggcc
    11341 ctggtccagg tacgcgctgc cgaagtcagc ctggcgcaga tgcggggaga gctgaccggt
    11401 gcggaggggg cgctggtcga ggcacggaag cgggaggccg agcgcggcga gaaggagaaa
    11461 gaagtcgcgg acctccgcat ccgcttgcgg ctccataacg agctcgaccg cgcccttggc
    11521 gacctccgca cgggcctgaa cgccgcgatg cggcccgaga tcgcgaccct cgcgtccagc
    11581 ttcctgtccg atctgaccga cggccggtac tcggatgtcg acctctccga ggattaccgg
    11641 gtgatcgtac tggacgagga gctgccgaag cccgtgttgt cgggcggtga ggaggacctc
    11701 gccaacctgg tgctccgtct cgccatttcc cagatgatcg ccgagcgtgc cggccagccg
    11761 ctctcgctcc tggtgctcga cgagatcttc ggctctctgg atgaagggcg ccggcaccac
    11821 gtcctcactc tgctgcggcg tttgggggac cggttcccgc aggtcattct catcacgcac
    11881 gtggagcaga tcagggatgg cctggaccgg gtgatccggg tggactacga ctccgggaaa
    11941 ggcaccagcg tggtgcggga cgacaccgcg acgctcggag gggccgatgc gggcgtggct
    12001 gcctgagcgc gaggtgcacg gccccgaacg ggccggcgag caggacatcg agggcctgaa
    12061 ccggctcttc gcggaggcgt tcaccgaccg gtaccgccgc gacggcctgg tgggcgtgcg
    12121 ggtgcctcgc ctcaatcccg cgatctggcg ctacgccctg atcgacgcgg gtgaaggcgc
    12181 catgctgtgg cgcgacgaac atggggacat cgccgccttc aacctcgcgc acctgtgcgg
    12241 ccgcgaagga tggatggggc ccttggcggt ccggaccgac cggcagggcg gaggggtggg
    12301 gaagagcgtg gtcggggccg ccgctgattg gctgatcgac cgcggggcgc acacggtggg
    12361 actcgagacc atgccccgga ccgtcgacaa catcgggttc tactcggggc tcggattccg
    12421 ccccgggcac ctcaccgtca cgctgacgct ggacaccagt acccgccggc tccccgcccc
    12481 ggcacttctc tccgaacaga ccagcgaccg gcagcactcc ggagtcacgg ccattcgcgg
    12541 attggtcgag gagcttgcgc ccgggtacga ctgctcgcgg gagatcttgt tgacgtcgga
    12601 gatgtcgttg ggggacacgg tgctggtgtg gggggacgcc gggctcgacg gttttgcgct
    12661 ctggcattcg gcgcccctgg ccgagggacg tgaaggggac gaggtacgcg tgctgaagct
    12721 cgcggcgcgg tcaccggagt ccttcacggc ggtggtgagc gccgtagagg ccgcggcgtc
    12781 gcggtcaggg atcagacgcg tggccatccg gtcgcagacg gcctacgctg atgcctatca
    12841 gtcactgata gcccgggggt atcaggtccg atggaccgat ctgcggatgt atctgaccgg
    12901 ctatccggag cggagtcccg cgaagggtgt gctgttctcg aattgggaga tttgaactgc
    12961 ggcgatcagc ctgctgacgg ctgacggctg atggcctctc acgcgctcag agataccagt
    13021 ttggcatcct tggttccgac caaatggatg gcggtaccaa gcccgataac ctcagtcgta
    13081 tgcggcgtgt ccgccggaag gtcgaggcgg tcgccgggtc ccaggtcgta ggtctccgcc
    13141 cccacacgga cccgcaggta tcccgacacc acccagcgcg tttccggcca gtgactggcc
    13201 tcatcgccgc tcttggcgcc gggcacgtcg gaccaccacg ccgtgtgctt gccctcgcgc
    13261 tgcagcatct cgcgaagagc cgacatgacg actggtcctt gctcaggggt ccatctctgc
    13321 aagtaggcca tcgtctccga acctagggcg ccgagcgggg gagcgcaatt gaggcgttcc
    13381 ctgaacgtct cgtcatgaca agaacttcgg gttgccgacc atgatccgcc gtgccacggc
    13441 gatcgccgcg atggcgtaaa gcaggttcac cgagccggat ccgatgtgca tgacgaacaa
    13501 caggaccgcc caggcgatca gtgacgcaat ggcgactatg agtgtgatgt tcatcaatcc
    13561 ttccttgacg aaagcgtccc gtgaggtatc gtagtcttgg ggacggagag cttcaactgt
    13621 cgagccaaca gctcggatgt ggacccagat actgtaacca atgtcatgcc ccctccgtgg
    13681 ggaagacaga agctgcggtg agggaccagt cattactttc gggcttcgac tttcctctcc
    13741 gttcctgatg ttccgatcgg ccgcggcgct cagcgcagcg gccgatcgtg tttctctgac
    13801 taaccgtcta accgtctaac cgactgccac gccgctctta aggccaccgg ccgccgccgt
    13861 cccgcgacgt agagcgtcag cagtaccgca atccagaccc cataccccca cgccacttcg
    13921 acccgccagt gcggcaccag gaactgcacc agccagagca cgaagatccc cgccgcctcg
    13981 taccactgaa actcgagggt caggagcacg agcaccgcga ccgtgctctg gaggagcgtg
    14041 atcaggatct cgagttcctg ctctccgtcg aagccgaagg cgctccaggt gccggtcgcc
    14101 cgccagtgcg aatacccgta gacgagcggg atcatgggtg ccaggacggt ccattggttg
    14161 atattcgagg acacgaggtt catcagggcc atcggtgcgt gggacacgcg ccgcgcccac
    14221 gcgaaggccg acagcttttc ggggaactcg gagaggaagg gcgcgagcca ttgcaccagg
    14281 acgaactggc tgacgccgag ggtgcccgcc agcgcgagca tcgactcgag gaaggggtgc
    14341 gcggtgaaaa agagcagggc cccgccgacg aggaagaggc cgccgatcgc cgccgggcgg
    14401 cgccagcccg gctgccggta cgcccattgc gcgacggcag gtgcgtcttc gacatgttct
    14461 ccggattccg gcggatgccg caggagggcc agcaggtacg cgccgtagag cgcgaggagc
    14521 accagggcgt cgatccacgt gatggtgcgc ttcgcgtaga tgaagccgaa gtacaagagg
    14581 ccggggatca ggccgacgac ttcagtcgcg tgctcggggt tgagccggat ggccggaagg
    14641 cgttcagtcc gctgccggcg gccataccag gcttgcacga catagatcat cggccatccg
    14701 agacccagca gcaggcggat ggcgccggtg aggttggcga tggcgaggtg ggcgcgcgcg
    14761 gggtcgtgtc cggcttccca cgcgatgacg gcttcgacgg cgaactcggg gagggtctgg
    14821 agccacgcga ggatggcgag ggccaacccc tgagacatca ggaactgcgc ggcttccgct
    14881 ccccatgcga cgaggaaggc ggacaacagg atgctgggga acgtccagag ggccgagagc
    14941 gcggaactgg ccgcgcctcc cgcgtgttgg acgaagcgga gtagctttag gactcgcacg
    15001 tgcgccgaag ataggggaac ccatggcccg cgaccagatg aagtcggctt ctcaccacag
    15061 cttccatgaa tcgcacacgc cgcccagcgt ggcgcgcatt cccttcgagc agctcatctc
    15121 cgagatgctc acccagctcg gggaggatcc gaaccgggag ggcctgctgc ggacgcccca
    15181 tcgggtggcc gagtcgatga agttcctcac ccgggggtac gggatgagcg tcgaagccgt
    15241 gatcaatgac gcgctcttcg aggaaacgca cgaatcgatg attctggtgc gggacatcga
    15301 gatctactcg ctctgcgagc accacatgct cccgttctat gggcgcgctc acgtggctta
    15361 cctgccgaac ggcaagatcg tcgggctgtc gaagatcccg cgggtggtgg atgtctttgc
    15421 ccggcggctt caggtgcagg agcgcctcac ggaccaggtg gcggatgccc tcatgcgcgt
    15481 cctggaaccc ctcggcgtgg gtgtggtcat cgaggcctat cacctctgca tgatgatgcg
    15541 cggcgtcgag aagcagaatt cgaagaccat cacgagcgcc ctgcgcgggg cgttccggga
    15601 tgacgccaag acccgcgaag agttcctggg cctggtcggg tagtgacggc actggcggga
    15661 aaggtcgcgc tggtcaccgg ggcctcccgc gggatcgggc ttggcgtggc caacgagcta
    15721 cgggccgcag gggcccacgt ggtgcggctg gcccgcagtc tgtcggatgg ctcgtccgag
    15781 cggcagacgg acttccggtg cgacgtctcg gactcaagcc aggtggagcg cgtcatcggc
    15841 caggtgctcc gcgacctccg ggcaccggac atcgtagtca acaatgccgg gattttcttc
    15901 atcaagcggc tggagggaac cacccccgcc gatttcgcgc aggccatcgg catcaacctg
    15961 acggggccgt ttctcatcgc ccgggcactc ctgccccatc tggtcaagcg ggggagcgga
    16021 gacctggtga ccatcggctc ggtctccgac cacgtcgcgc tcccgggctc ggcggcctac
    16081 gcggccagca agtttgggct ccgagggatg cacggctcga tcgccgcgga gctcgcggga
    16141 acgggcgtgc gggccacgct ggtgtcgccg gggccggtgg ataccgagat ctgggatccc
    16201 atcgatcccg attccaggac ggggttcacc aagcggaagg acatgatgcg cgccgaggac
    16261 gtggcggagg cggtgctctt cgcggtgacg cggcccgccc ggaccgcggt gacggagata
    16321 cggctgcttc caaccggata ctcggcgagg ggatgatgaa gcgggagtcg ggagtcgggg
    16381 gtcgtctgct cccagtcatt atggggctcc tcatggttgg gccgctcgcc gcgcaaggcg
    16441 tcgacgagat tcccgattcg acaggctggg gcctttggag tacggcgatc ctgaccctcg
    16501 cccatgcacc ggacagtgcc acgtgggtcg gcacgtacgg gcacgggatc ttcgtcctgc
    16561 gcccgcatgc gaccgcgtgg gagcacatct ccgaaagcga cgacacgtcg aagcattcga
    16621 tctcgtggga cttcgtccac gcattcgcgt tcggcccgaa gggcgaggtc tggtacggga
    16681 ccgtagggaa tggctgggga ctttcgctcg ataacgggaa gacgtggaag aactggcaat
    16741 atcgggagct tggccctgag tggcagtatg tcgcgccgaa cgggattgcc acgcgcgggg
    16801 acacgaccta catcgccacc gctgacgggc tcaagatcac gggggatgac ggaaggacct
    16861 ggcttgttgt cacggactcg atgggtgcga cgacggccaa ggactccgtc atcgggcgga
    16921 tcaggaatca gtacttgttt gcgatcattg tgggtgacgg tggcaacatg tgggtcagcg
    16981 agctgacggg tattgctcat tcgcccgatg gggggcggac gtggcatgag gggttcgtca
    17041 gaccgccatg tcgaggggtt ggctgtgcca atcgagcacg tgccttcaga ggcgatccgg
    17101 gcaaaccact atgggttgca accgaggatc ttgcttatca gttcgcgccg cagttcggcg
    17161 gctggaaatc cctcattccc gacagcctgg tcgcacgact gcctcctgac accgccgagt
    17221 tcgtggccga tacagtgatc cccgcggacc ccggggactg tggcggcgga attcgggtgt
    17281 tcgacgggcc ttgccccgag ccgcatccga gacgtggtgc cccttgggct gcgcgaccgg
    17341 ctggactccg gcacacgtgg ttccgccgcc cgatcgccct cgccgaccag ccgtacatcg
    17401 accagaccta ccgctttggc tcgacgatgg gcggcaactt ccaggcgcat cagggcgtcg
    17461 aattcaacaa ccccgatgga acaccggtgc acgcgattgg ggacggggtc gtgatctacg
    17521 ccgggaaggc tgaggctggc gccaacaccg tggccatccg gcatgaccgg cggttttcgt
    17581 taaccccggg gcatggcccc ggggttaacc cggcctccca gtacgtcttt tccgtctact
    17641 accacaactc gaagctcctg gtgaagaccg gagatcgagt caaagccggg gacgtcgtct
    17701 ccctcgtcgg caacaccggc cgcgccac
//
LOCUS       DASUBP010000246         7327 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_662607, whole genome shotgun sequence.
ACCESSION   DASUBP010000246 DASUBP010000000
VERSION     DASUBP010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7327)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7327)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7327
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_662607"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(778..1302)
                     /locus_tag="VFU75_09360"
     CDS             complement(778..1302)
                     /locus_tag="VFU75_09360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467088.1"
                     /translation="MLALWVPLWGALVAPVAPSSAQAPDSAVLHRYADAVYATADTLD
                     VLRGVTQAFRNDLKTASPTLVLARATSVQNACSSGVTAVRQLQGMLAARTVTPKSAPS
                     QAKFRTAAEETVAALERCVRNWQPLPRTDARADTLRAWGPYRVNEIEVALRRFDLARF
                     TFAAAAGLPKRPVQ"
     gene            complement(1302..1922)
                     /locus_tag="VFU75_09365"
     CDS             complement(1302..1922)
                     /locus_tag="VFU75_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467089.1"
                     /translation="MTVPATSRIVRTFDDRADALAHFFQRAGEAPRLLAFDDSVGCPL
                     DQAVAAIEWTAAVGILAEDDLLHAARVSADAAAAVVERRDGDSRVFVYFGPQMDAPPA
                     DPYEGTLLYDEPGVRAYIFAQRVHAVAHFLRATQGLGAVLSMLSRRAPELRHVRRWIQ
                     ALFAEPVGHHSTQLLGGWFATSGAGCLFLPPQPGEPLTYCEVGIDS"
     gene            1979..2524
                     /locus_tag="VFU75_09370"
     CDS             1979..2524
                     /locus_tag="VFU75_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467090.1"
                     /translation="MRRLVVLLSVTGSLVASPLVAQDTTPTPKPPDSTHHDSVPPPTA
                     DQLKWMEGLRTAARGIGQIKSGIETVQRAGTSPTQLKRAGKLLAGFCGTGRGFMHTGV
                     ARMSSTAYPPGDNRKIAKQVVIQIDSVAKETTSCEQTAARDPKTSVQHLTPLLMSYEA
                     SIAAWRAMVGLPAIPTRNTKP"
     gene            2829..3029
                     /locus_tag="VFU75_09375"
     CDS             2829..3029
                     /locus_tag="VFU75_09375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002633565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEU5467091.1"
                     /translation="MAKGKVKWFNDAKGFGFIAQESGPDVFVHFTAIQAEGFRSLAEG
                     DSVEFEVAQGPKGLQAQNVRKV"
     gene            3118..3657
                     /locus_tag="VFU75_09380"
     CDS             3118..3657
                     /locus_tag="VFU75_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467092.1"
                     /translation="MRALTLALALAPGLVAAQSQPPAAPAALLPYRGFRPGVSAAEFE
                     TRARSLTRAGATPLVCNTSTRTAQVMECGVIIHDPDDGAEFYLSGHFIDGRADVVSFG
                     DSGTSALVIKVQRELRARYGPPHPVGTASWQWRQGAQFVRLTWRARGPRRWIFIQLTD
                     TDVMRGIGHYVPREAPKKS"
     gene            complement(3658..4500)
                     /locus_tag="VFU75_09385"
     CDS             complement(3658..4500)
                     /locus_tag="VFU75_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467093.1"
                     /translation="MDQVATLFQFTGALERLLTTRDTAAFEAVWDAEELARVGWEALA
                     VARHPDSEPLERGLAALDRRLLAALARARALRDLHTSTFRVPELERWQHAAAAALVAS
                     RWGSAGLRTVISDPLAPVARRYFALLALAERHPPAAGPLFQRYLRTPASHHAFVAAAV
                     EAARWYPDQVDDLLSLFDRIRSEQLRRRFLGPKILGSLYYQGNARALPLFQELCISGH
                     TGGDADCCEVTRALVAVRRLTGVVPLNVKYPDPQMPEVRDALDVAEAAYEAERGRLAP
                     VTVI"
     gene            4555..5697
                     /locus_tag="VFU75_09390"
     CDS             4555..5697
                     /locus_tag="VFU75_09390"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5467094.1"
                     /translation="MPRFTPSEDVLRNLALLCAVTLSVACAGSSGTITIAVSGDVKEA
                     SGVSMQAAAELAASQINARGGIGGRHLDIRFLPDSGDPNVAVRIARRLVDDGSVVAVV
                     GHVTSGITQAAMGVYGSGRNPLPVVSPTASSPDLSGVSPYFFRTCPTDLAHGPALARF
                     ARNTLHASRVGVLFENDDYGRGMKRAFSREFRQLGGTIVSEDPFFATTPSLEPYLARI
                     SRDGGADVLVVGADPEGGTLALRDLRRLGSKVPVIGGDGLAGIEADSALAEGTRISLS
                     YLADRPGAANANFVAAYARANHGDRPNDTGAETYDAVNLLAKAIEQVGPDRRAIRDYL
                     AQVGRGRPAFEGVTGTIAFDSLGDVPAKAVVIGVVHSGHLELDRTR"
     gene            5694..>7327
                     /locus_tag="VFU75_09395"
     CDS             5694..>7327
                     /locus_tag="VFU75_09395"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HEU5467095.1"
                     /translation="MIARFDTLRWRIVAGFASLVLLLLIGSGAGIYSLWKMRDAVAQE
                     LARLQASNQISTAVMASTFAEIRAAEQYLNEPTAAAAADFHQNADSAFADAKDLSGIE
                     GLTVEDRLAVNRVQQIQGQIEVTYSLAHALSDIGRAPQAEQEAQQARGPARELTTILL
                     SLGARQADRSRQATDRLGSLARRMTVLLSGVLVLAVVGGTLIGWGTLTMVEAPLRRLA
                     TAAERFGAGDLRPMSTGKMPSEFEVLAAAIRDMGERLRGIVTEVITEADRIATSAGDL
                     SAVSEELAASSAEVSTAMVEISSGAEKQRNELNEMGAGLAQLKAAAAEIADAAERVTK
                     LGEEIRNVADRHRHDVMSASTALLEVREVVQTTSSQVTALAQQSAAIDDFVELIKRIS
                     SQTNLLALNAAIEAARAGEHGRGFAVVAQEVRQLADESARAAEDVAQTTTAIRKRVDE
                     VTVTMGAGQTKVHGIETTARGAATGLSEIVSAVEEVERAAAKVRQAAGINREVTDHIG
                     ERAQQVSTRAVNHAAGAQEVSAAAEEQGASTEEMAAS"
BASE COUNT         1239 a         2462 c         2479 g         1147 t
ORIGIN      
        1 acgtgttcag tgaccccgcc caagtggggg tagtagaact ccgtgaccag agcgatgcgc
       61 atgtgagcga agggctcccg gggcgggggc aagccaacaa cgtaatgtgc gccccgagca
      121 aagcgagggg tgcaccctga gcgcagcgaa gggtcttggc gtaaggtggg cgtaaagaaa
      181 gccgaagccc ccggatggtt tcccacccgg gggcttcgcg caccggctac ccggagccag
      241 gggatttact cccccgttct tcttcccgcc gccgccggtt gactgttgcg tcattcggta
      301 gggcgaacag gagctccggc ttcgatcccc aagcagcctg gccgggccgc aaggttcacc
      361 agtgtgagtg ctgcgggtca gatcgggtcg cggaccgacc ttccgcactg cacgccgagg
      421 cactacttcg acgtggatga agcaacgcga ctggctccat ctgtccgagc cggagagagc
      481 actctcccgg atcagcaggc gcctgagccc ttgtttgcgt cggcgccctc cccggaattt
      541 ccgcttggac ccaccttaaa ggcgttgtgg aagcgctggg cctccaccct tcccctcaag
      601 gcgcgtgaaa gccaatatag gagcgcctga aagtggcgca agtgccgata tttcttcggg
      661 ttagctcatt tcgatgtgca cggcgtgtgg aaggtaagac acggttgagc cggtggttct
      721 ggcgttcatc cacacaatgt ggagttgtgt atccacagtg tgcacgttcc cacatcgcta
      781 ctgaacgggg cgtttgggca gtccggccgc cgcggcgaac gtaaaacggg cgagatcgaa
      841 cctcctgagt gccacctcga tctcgttgac gcggtaggga ccccaggcac ggagggtgtc
      901 ggcgcgtgcg tcggtgcggg gcaacggctg ccagttgcgg acacagcgct cgagtgcggc
      961 gacggtctct tccgcagcgg tccggaactt cgcctggctg ggggccgatt tcggagtgac
     1021 cgttcgggcc gcgagcatgc cttgcagctg acgaacggcg gtaaccccgg aggagcaggc
     1081 gttttgcacc gaggtggccc gggcgagcac gagcgttggt gatgccgtct tcagatcgtt
     1141 gcggaacgcc tgcgtcactc cgcgaaggac atcgagcgtg tccgccgtgg catatacggc
     1201 gtcggcgtac cggtggagaa ccgcgctgtc aggggcttgg gcggaagatg gagcgacggg
     1261 cgcgacgagg gcgccccaca gcggtaccca gagtgctagc atcaggagtc gataccgact
     1321 tcgcagtagg tgaggggttc gccgggttgc ggcggcagga agagacagcc ggcaccactg
     1381 gttgcgaacc aaccgccaag caattgggta gagtgatgcc ctacgggctc ggcgaatagc
     1441 gcctgaatcc agcggcggac gtgccggagc tcgggagccc ggcgggacag catactgagt
     1501 acggcaccga gcccctgcgt ggcccgaagg aagtgggcga cggcgtggac gcgctgcgcg
     1561 aagatgtagg cgcggacacc cggttcatcg tacaggagag tgccttcgta gggatcggct
     1621 gggggcgcgt ccatctgggg tccgaagtag acgaagacgc gcgagtcgcc atcgcggcgc
     1681 tcgacgacgg ccgcagcagc atccgcgctc acccgggcag cgtgcaagag atcgtcctct
     1741 gccaggatgc cgaccgcggc ggtccattcg atggccgcga cggcctggtc gagggggcag
     1801 cctacactat cgtcgaaagc caggagacgc ggcgcttcgc cggcccgctg gaagaagtgg
     1861 gcgagtgcgt cggcccggtc gtcgaaggtg cggacgatgc gcgaggttgc gggaacggtc
     1921 attgcgtgat tataaccctg tgccggggac ccagacagac cttgttcagg agatgcgaat
     1981 gcgtcgactc gtcgtgttgc tgagcgtgac tggttctctc gtcgccagcc ccctggtggc
     2041 ccaggacacc acgcctacgc ccaagccacc cgattcgaca caccacgact cggtcccgcc
     2101 acccaccgcc gatcagctca agtggatgga gggactgcgc acggcggccc gtgggatcgg
     2161 ccagatcaag agcggcatcg agaccgtgca acgcgccggc acgagcccca cccagctcaa
     2221 gcgggccggc aagcttctcg ccggattctg cgggacgggc cgcggattca tgcacactgg
     2281 cgtcgcgcgc atgagttcca ccgcttatcc ccccggcgac aaccggaaga tcgcgaagca
     2341 ggtggtcatc cagatcgact cggtcgccaa ggagaccacg agctgtgaac agacggcggc
     2401 cagggatccc aagacttcgg ttcaacacct caccccgctc ctcatgagct acgaagcgtc
     2461 catcgcggcg tggcgcgcga tggttgggct ccccgccatc ccgacacgga acaccaagcc
     2521 ataagatatt tcccgatatc gtcttatagt tccatcgtcg aataagtgtc gttatggtgt
     2581 cgctatttag ccagataccg agcgaaccgt gcaagccgtt tgtatgtggt cgtttacgtt
     2641 gcttgacacc cccaaaaacc acctctactt tcagccccgg ttcaagcaag acgtggcacc
     2701 gtcaccggtt agactgcgca ccgcgagaac caacaggtct cgcggttttt gtttgagccc
     2761 gtcgctgatc gcggtggtca gcgccgcact attccggggc agtgcccctc agttcgagga
     2821 tgtaacagat ggccaagggc aaggtgaagt ggttcaacga cgcgaaaggc ttcggcttta
     2881 tcgcgcagga gtcgggacca gacgtgttcg tccacttcac ggcgatccag gcggaagggt
     2941 tccgtagtct cgcggagggc gacagcgtgg aattcgaggt cgctcaggga cccaaggggc
     3001 tgcaggcgca gaacgtccgc aaggtttgat gtagcaccgc aggatcgttg gagggacggg
     3061 cccggtgacc atcaccgggc ccgtttttca ttttcaaccc tatattgtcc ttcgaccatg
     3121 cgcgcgctga ctctcgctct cgcactcgct cccggcctcg tggccgctca gtcccagccg
     3181 cccgctgcgc ccgccgccct gctgccctac cgcgggttcc gcccgggcgt ctccgccgcc
     3241 gagttcgaaa cgcgcgcgcg gtcgctcacc cgggcgggcg cgacgcccct cgtgtgcaac
     3301 acgtcgaccc gcacggctca ggtgatggaa tgcggcgtca tcatccacga tccggatgac
     3361 ggggcggagt tctatctcag cggacatttc atcgacgggc gggcggacgt ggtctcgttc
     3421 ggagactcgg gaacgagtgc gctggtgatc aaggtccagc gcgagctgcg ggcccgttac
     3481 ggtccgcccc atccggtcgg gaccgcttcg tggcaatggc ggcagggtgc ccaattcgtc
     3541 cggctcacct ggcgggcacg gggaccacgg cgctggatct tcatccagct caccgacacc
     3601 gacgtcatgc gcggcatcgg gcactacgtg ccccgggaag ctccgaagaa aagctgatca
     3661 gatcaccgtg accggcgcca gccggccacg ttccgcctca taggccgctt ccgccacatc
     3721 cagcgcgtcc cgcacctcgg gcatctgagg atccgggtat ttcacgttca gtggcaccac
     3781 acccgtcagc ctgcgcaccg ccaccaaggc ccgcgtcact tcgcagcaat ccgcgtcgcc
     3841 cccggtatgg ccactgatgc agagctcctg aaacagcgga agtgcccggg cattcccctg
     3901 ataatagagt gagccgagaa tcttcgggcc caggaagcgc cgccgcagct gctcgctcct
     3961 gatccggtcg aagagcgaga ggagatcgtc gacctggtcg ggataccacc gggccgcttc
     4021 gacggccgcg gccacgaacg cgtggtgcga cgcaggagtg cgcaggtaac gctgaaagag
     4081 gggtcccgcc gccggtgggt gccgctcggc cagggccagg agcgcgaagt accggcgggc
     4141 gaccggcgcg agcgggtcgg agatgacggt ccgcaatccc gcgctgcccc accgcgacgc
     4201 gaccagcgcg gcggcggccg cgtgttgcca gcgttccaac tcgggtacgc ggaacgtgct
     4261 ggtgtgcagg tcccgcagcg cgcgggcccg tgccagcgcc gcgaggagac gccggtcgag
     4321 ggccgcgagc ccgcgctcga ggggctccga atccggatgg cgggcgacgg caagcgcctc
     4381 ccaccccacc cgcgcgagct cctccgcgtc ccagaccgct tcgaacgccg ccgtgtcccg
     4441 cgtcgtaagc aggcgttcga gtgcgccggt gaattggaag agcgtggcga cctgatccac
     4501 gagcccaaat tatcccccca ccggcattgc ggaacccctt gcacggcctt aaccttgccg
     4561 cggttcaccc cctcggagga cgtcctgcgg aatctggccc tgctctgcgc cgtgaccctg
     4621 tcggtcgcct gcgccgggtc gagtggtacc atcacgatcg ccgtgtccgg ggacgtgaag
     4681 gaagccagcg gggtgagcat gcaggccgcc gcggagttgg ccgccagtca gatcaatgcc
     4741 cggggaggca tcggcggccg ccacctggac atccgcttcc tgcctgactc cggcgacccc
     4801 aacgtcgccg tgcgtatcgc ccggcggctc gtggacgatg gctccgtggt ggcggtggtc
     4861 ggacacgtga cctccgggat cacccaggcg gcgatgggcg tctacggcag tggccggaac
     4921 cccttgccgg tcgtctcgcc gaccgcttcc agtcccgacc tgtccggcgt ttccccctat
     4981 ttcttccgga cctgccccac cgacctggct cacggaccgg ccctcgcgcg tttcgcccgc
     5041 aacaccctgc acgcctcccg ggtcggcgtc ctgttcgaga atgacgatta tggccgcggc
     5101 atgaagcgcg ccttctcgcg ggaattccgc cagctgggtg gcacgatcgt ctccgaggac
     5161 cccttcttcg cgacgacccc gtcgctcgag ccctatctcg cgcggatcag ccgggatggt
     5221 ggtgcggacg tgctcgtcgt cggtgccgac cccgaaggcg ggaccctcgc cctccgggat
     5281 ctgcgccgcc tgggcagcaa ggtaccggtg atcggtggag acggtctggc cggcatcgag
     5341 gccgactcgg cgctcgccga gggcacccgg atctccctgt cgtatctcgc ggaccggccc
     5401 ggggccgcga acgccaactt cgtcgccgcc tacgcgcgcg ccaatcatgg cgaccgcccc
     5461 aacgacaccg gcgcggagac ctacgacgcc gtgaatctgc tggcgaaggc catcgagcaa
     5521 gtgggccccg atcgccgcgc catccgcgat tacttggcgc aggtggggcg cggccgcccc
     5581 gcgttcgaag gcgtcaccgg gaccatcgca ttcgactccc tgggagacgt gccagccaaa
     5641 gccgtggtga tcggcgtcgt gcacagcggt catctcgagc tcgaccgcac ccgatgatcg
     5701 cccggttcga caccctccgc tggcggatcg tcgccgggtt cgcgtccctg gttctgctcc
     5761 tcctcatcgg ttcgggcgcc ggcatctatt ccctctggaa aatgcgcgat gcggtcgcgc
     5821 aggagctggc ccggcttcag gcttccaacc agatctcgac cgccgtgatg gcgtcgactt
     5881 tcgccgagat ccgcgccgcc gagcagtact tgaacgaacc cacggccgcc gccgccgcgg
     5941 acttccatca gaacgcggac agcgcgttcg cggacgccaa agacctgagc ggcatcgaag
     6001 ggctcacggt cgaagaccgc ctcgcggtga accgcgtgca gcagatccag ggccagatcg
     6061 aggtgaccta ctccctcgcc catgcgctct cggacatcgg ccgcgccccc caggccgagc
     6121 aggaagcgca gcaggcccgt ggccccgccc gcgagctcac caccatcctg ctctccctcg
     6181 gcgcccggca agctgaccgc tcccgccagg ccaccgatcg cctcggctcg ctggcgcggc
     6241 gcatgaccgt cctcctgagc ggcgtcctgg tgctcgcggt ggtcggtggg acgctgatcg
     6301 gctggggcac cctgacgatg gtcgaggcgc ccctccgccg gttggccacg gccgccgagc
     6361 gcttcggcgc cggtgacctg cggcccatga gcaccgggaa gatgccgtcc gagttcgagg
     6421 tcctggcggc cgccatccgg gatatgggag agcggctccg cgggatcgtg accgaagtga
     6481 tcacggaagc ggaccgcatc gcgacgtcgg caggggatct gtccgcggtc agcgaggagc
     6541 tggcggcgtc cagcgccgag gtatcgacgg cgatggtcga gatctcatcc ggcgccgaga
     6601 aacagcgcaa cgagctgaac gagatgggcg cggggcttgc ccagctcaag gccgccgcgg
     6661 cggagatcgc tgacgcggcg gagcgggtca cgaagctcgg tgaagagatc cgcaacgtcg
     6721 ccgaccgtca ccggcacgac gtcatgtccg cctctactgc cctgctcgaa gtgcgggagg
     6781 tggtgcagac cacgtccagc caggtcacgg cgctggccca gcagagcgcc gcgatcgacg
     6841 acttcgtcga gctgatcaag cgcatctcgt cgcaaaccaa cctgctcgcc ctgaacgccg
     6901 ccatcgaggc ggcccgggcc ggcgagcatg gccgcgggtt cgcggtagtg gcgcaggagg
     6961 tccggcagct ggcggacgag tccgcgcgcg ccgcggagga tgtggcgcag accacgacgg
     7021 ccatccggaa acgcgtggac gaggtcacgg tcacgatggg agccgggcag accaaggtcc
     7081 acgggatcga gactacggcc cgtggcgccg ccacaggcct gtcggagatc gtctcggcgg
     7141 tggaggaagt ggagcgggcg gcggccaagg tccgccaggc cgccggcatc aatcgtgagg
     7201 tcaccgacca catcggcgag cgggcccagc aggtgtcgac ccgcgcggtg aaccatgccg
     7261 cgggagctca ggaagtcagc gccgccgccg aggaacaggg cgcgtccacc gaagagatgg
     7321 ccgcttc
//
LOCUS       DASUBP010000247         4342 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_308082, whole genome shotgun sequence.
ACCESSION   DASUBP010000247 DASUBP010000000
VERSION     DASUBP010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308082"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..1041
                     /locus_tag="VFU75_09400"
     CDS             235..1041
                     /locus_tag="VFU75_09400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467096.1"
                     /translation="MKPRLARVLLGVASFLLASRADGQTAPGYRYTFTFSGDGADPVS
                     GTTWAAGQRSRIDIDGQSDPQYILVDNAAHTVTLVYPDRKEYSVVSDTTFQRLVGTVL
                     ASLPVVNITLSDTQVSDTALGRGEVIAGHPTDRYQVTQAFTVSVGAFGFQAMGMRQRV
                     TTEYWMARDLKLPSNPLVTLLAQLTTVLAQSDRDFAHRSAVAMRPVVRGTPLRVVVHS
                     ETRSGDKNEVTQKTQTLQVTSIAPASVDLSRFVLPAGYTQKDPQFTGKIF"
     gene            1103..1184
                     /locus_tag="VFU75_09405"
     tRNA            1103..1184
                     /locus_tag="VFU75_09405"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1137..1139,aa:Leu,seq:gag)
     gene            1242..2972
                     /gene="sulP"
                     /locus_tag="VFU75_09410"
     CDS             1242..2972
                     /gene="sulP"
                     /locus_tag="VFU75_09410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate permease"
                     /protein_id="HEU5467097.1"
                     /translation="MLVPKLITTLRGYTRDQLTADLIAGVIVGIVALPLAIAFAIASG
                     VTPEKGLYTAIVAGFLISALGGSRVQIGGPTGAFVVVVYGIVQRYGVEGLTVATLMAG
                     CILVVFGLVRMGGVIKFIPYPVTLGFTAGIALIIFSGEVKDFLGLRMGPPPADFIPKW
                     RALVAHLPSANPWAVGLSMATVLIILLWPRVSRRIPGAFVALIVTTAAVRWGGIPVET
                     IGSRFGHIDAGFPSPQIPFVTLDTMRGLIGPAFTIAMLGAIESLLSAVVSDGMIGGRH
                     RSNMELVAQGVANLASPLFGGIPATGAIARTATNVKNGGRTPVAGMTHALTLLLITVF
                     FGRWAALVPLATLAGILVVVSYHMSEWRSFRMELRAPRSDLLVLLTTFGLTVVVDLTV
                     AIEVGMVLAAFLFMRRMAEVANIRVLTHEFADPANDDEGDPNAIQRRQVPAGVEVYEI
                     SGPFFFGAAETFKDRLSQIAGKPQVLILRMRHVPAIDSTGLNALRDLVRRCRQEGTFV
                     LLSDVHSQPVVALERSGFYGELGEANVTGNIDDALNQARAHLGLPAVPKPAFAAPTVA
                     RESGEARARP"
     gene            complement(2969..>4342)
                     /locus_tag="VFU75_09415"
     CDS             complement(2969..>4342)
                     /locus_tag="VFU75_09415"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU5467098.1"
                     /translation="QGYWYPEIAVYDDVDGWVADPYLLEAELYMDHADYDVRLTVPRG
                     WVVGATGTLMNPEGVLSKAARDSVAVARRTGRVVRVLTPGPGAAGAFATSGPTTTWHY
                     VANDVRDFAWGSSDQYAWDQTRALIDSGRDTVNISSYFRLTAPAAAWRVGGARYTRDA
                     IEQMSAYLWKYPWPQMSSMEGVLDGGGMEYPMMTLMQPWADTLSLAGDLMHETGHMWF
                     PMQAGSNETRFPWMDEGFTQFDVAQGMKVLYGAPRQGGRPNDSEEGQRAGYTRAARAG
                     QDHSLMVPGDEFPQDLYFTMYYNKTGQILAALRGLVGNDAFHRSFVAYGREWAGKHPE
                     PYDFFNAMRAGTGRDLSWFFTTWFYHGWPLDQGVDSVIAVGGGDSTEIVICDMGLAPM
                     PVPLAVTRSDGTVQRVDIPVDTWWANGGSRRARIRVATLPAITRVEIDPDQLFPDVRR
                     ENNVWKR"
BASE COUNT          673 a         1471 c         1424 g          774 t
ORIGIN      
        1 tcgtggcaga acgagcactc gaggcaatgg gtgaagtact gcctcgagaa gatggcggga
       61 tcgacggcgg cgatgaccgg cgcgccgtag cggcaggggt aggattgcga gagggcgctg
      121 acggtcacac gaacaagttg ccaggggcca ggggccaggg gccaggggcc aggggtcggg
      181 ggccaatggc tcggaccttg gaacccaccg gaaggaaggg gcgtactgaa tctcatgaaa
      241 cctcgactgg ctcgagtgtt gctgggggtc gcatcattcc tgctggcatc gcgagcggac
      301 ggccagacgg ctcccggcta ccgctacacc ttcaccttct cgggggacgg tgccgacccc
      361 gtatcgggga ccacttgggc agccggtcag cgctcccgta tcgacatcga tgggcagtcc
      421 gatccccagt acatcctggt ggacaacgcg gcgcataccg tcacgctggt gtacccggac
      481 cggaaagagt actccgtcgt gtcggacacg acgtttcagc ggctggtggg gaccgtcctg
      541 gcgtcactcc cggtcgtgaa catcaccctc tcggacaccc aggtatccga cacggccttg
      601 ggcaggggag aggtgatcgc gggacacccg accgaccggt accaggtgac ccaggcgttc
      661 acggtgtcgg tgggggcgtt cggatttcag gcgatgggca tgcgccagcg ggtcaccacc
      721 gagtactgga tggcgcgcga cctgaagctg ccctccaacc cgctggtcac cctgctcgcg
      781 caactcacca cggtgctggc gcaatctgac cgggactttg cccaccggtc cgcggtcgcc
      841 atgcgccccg tcgtccgggg cacgcccttg cgggtggtcg tgcactccga gaccaggtcg
      901 ggggacaaga acgaggtcac ccagaaaacc caaaccctcc aggtgacctc gatcgcaccg
      961 gcgtcggtgg atctgagccg gtttgtcctg ccggccgggt acacgcagaa ggatccccag
     1021 ttcacgggga agatcttctg agccctcagc tgatggctgg tggcttgctc cccctccggc
     1081 ccccatctag ctttctcgac ccgccctggt ggtgaaactg gtagacacgc tgtcttgagg
     1141 gggcagtgcc gcaaggcgtc tcggttcgaa tccgagccag ggcatccgca atactttctt
     1201 catgaagtct tcaagggggc agcgctactt tcccgccccc gatgctcgtt cccaagctca
     1261 tcaccacact ccgcgggtac acccgcgatc agctcacggc cgacctcatt gccggcgtca
     1321 tcgtggggat cgtggcgctt ccgctcgcca tcgccttcgc gatcgcctcc ggggtcactc
     1381 cggagaaggg cttgtacacg gccatcgtgg ccggcttcct gatttcggcg ttgggcggtt
     1441 cgcgggtgca gatcggcggt cccaccgggg cgttcgtggt ggtcgtctac gggatcgtcc
     1501 agcgctacgg cgtcgaggga ctgaccgtcg caaccctcat ggccggctgc atcctcgtgg
     1561 tgttcgggct ggtgcggatg ggcggggtga tcaagttcat tccctatccg gtgactctcg
     1621 gtttcaccgc gggcatcgcg ctgatcatct tctccggcga ggtcaaggat tttctgggcc
     1681 ttcggatggg accgcccccc gccgacttca ttcccaagtg gcgggccctc gtggcccacc
     1741 tcccctcggc caacccctgg gccgtcggat tgtccatggc gacggtgctg atcatcctcc
     1801 tgtggccccg cgtcagcagg cgcatccccg gcgcgttcgt cgcgctcatc gtcaccacgg
     1861 ccgccgtgcg gtggggcggg atccccgtcg agacgatcgg gtcgcgattc ggccatatcg
     1921 atgccgggtt cccgagcccg cagatcccgt tcgtgacgct cgacaccatg cggggactca
     1981 tcggccctgc gttcaccatc gccatgctgg gggcgatcga gtcgctgttg tcggcggtcg
     2041 tatcggacgg catgatcgga ggacggcacc ggtccaacat ggagctggtc gcccagggag
     2101 tcgcgaacct cgcgtctccg ctgtttggcg ggattccggc gacgggggcg atcgcccgca
     2161 ccgcgaccaa cgtcaagaac ggcgggcgca cgccggtcgc cggcatgacg catgcgctga
     2221 ccttgctcct gatcaccgtc ttcttcggac ggtgggccgc gctggttccg ctcgcgacgc
     2281 tcgccggcat tctggtggtc gtgtcctatc acatgagcga gtggcggtca ttccggatgg
     2341 agctgcgggc tccccgcagt gatctcctgg ttctgctcac gacgttcggc ctcaccgtgg
     2401 tcgtcgatct cacggtggcc atcgaggtgg gcatggtgct cgccgccttc ctgttcatgc
     2461 gccggatggc cgaggtggcc aacatcaggg tgctgaccca cgagttcgcc gatccggcga
     2521 acgacgacga gggagatccg aacgcgattc agcggcggca ggtgcctgcc ggcgtcgagg
     2581 tgtacgagat cagcggacca ttcttcttcg gcgcggcgga gaccttcaag gaccggctgt
     2641 cgcagatcgc ggggaagccg caggtcctga tccttcgcat gcgacacgtc ccggccatcg
     2701 actcaacggg gctcaacgcc ctccgcgatc tcgtccgccg ttgccgccag gaaggcacgt
     2761 tcgtattgct gtcggacgtg cattcacagc ccgtcgtggc cctggagcgt tcgggcttct
     2821 acggtgaact gggggaagcg aacgtcacgg gcaacatcga tgacgccctg aaccaggccc
     2881 gtgcccacct cggtcttccc gcggtcccga aaccggcctt cgcggcgcct acggtggcgc
     2941 gcgaaagcgg cgaggcgcgg gctcgcccct agcgcttcca gacgttgttc tcgcgccgca
     3001 catccgggaa cagctgatcg ggatcgatct ccacccgcgt gattgcgggc agcgtcgcca
     3061 cccgaatgcg cgcgcgccgc gacccgccgt tggcccacca ggtgtcgacc ggaatgtcca
     3121 cccgctgcac cgtgccgtcg cttctggtca cggccagtgg caccggcatc ggtgccaggc
     3181 ccatgtcgca gatcacgatc tcggtcgagt cgcccccccc gacggcgatg acggaatcga
     3241 cgccctgatc gaggggccag ccgtggtaga accaggtcgt gaagaaccag gacaggtcac
     3301 gtcccgtacc ggccctcatc gcgttgaaga agtcgtacgg ttccgggtgt tttccggccc
     3361 actcgcggcc atacgctacg aatgagcggt ggaacgcgtc attcccgacg agaccgcgca
     3421 acgcggcgag gatctgtccg gtcttgttgt aatacatcgt gaaatagagg tcctggggga
     3481 attcgtcccc cgggaccatc aatgagtggt cctggcccgc gcgcgccgcg cgcgtgtagc
     3541 cggcgcgctg cccttcctcg gaatcgttcg gccggccgcc ctgccggggg gcgccgtaga
     3601 gcaccttcat cccctgcgcc acgtcgaatt gcgtgaagcc ctcgtccatc caggggaacc
     3661 gtgtctcgtt cgatccggcc tgcatcggga accacatgtg acccgtctcg tgcatcaggt
     3721 cccctgcgag cgaaagcgtg tcggcccagg gctgcatcag cgtcatcatg gggtattcca
     3781 tccccccgcc gtccagcacg ccctccatcg agctcatctg cggccacgga tacttccaga
     3841 ggtacgccga catctgctcg atggcgtcgc gggtgtaacg cgccccgccc acgcgccagg
     3901 cggcggccgg agcggtgagc cggaagtagc tcgagatgtt gaccgtatcg cgtcccgaat
     3961 cgatgagcgc ccgggtctgg tcccacgcgt actgatcgct cgatccccac gcgaagtccc
     4021 ggacgtcatt ggccacgtag tgccacgtgg tggtcggacc ggacgtggcg aatgcgccgg
     4081 cggctcccgg acccggcgtc agcacccgca cgacccgtcc ggtccggcgg gccacggcga
     4141 cgctgtcccg tgccgctttc gacagcacgc cttccggatt catcagggtg ccggtggcgc
     4201 cgaccaccca gccccgcggt accgtcagcc gtacgtcgta gtcggcgtgg tccatgtaca
     4261 gctcggcttc caggaggtac ggatccgcca cccagccgtc gacgtcgtcg tacacggcga
     4321 tttccggata ccagtagccc tg
//
LOCUS       DASUBP010000248         3897 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_553646, whole genome shotgun sequence.
ACCESSION   DASUBP010000248 DASUBP010000000
VERSION     DASUBP010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3897)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3897)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3897
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_553646"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            98..883
                     /locus_tag="VFU75_09420"
     CDS             98..883
                     /locus_tag="VFU75_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5467099.1"
                     /translation="MKDRYGIELCGVGKAYGSRVALSGLDLNVAAGEVVALVGPNGAG
                     KSTTLRILSGAIHPSAGIARIGGRDVSLDPLGARERLGYLPQRLGVPLSTVVRDLAAL
                     VSAARGLPADAGARALAEAHLGNRLDATLGQISGGQRQRVMLVLATLGAVEVLLLDEP
                     SISLDTEGAEEVRDAISAARFDGAAILFASHHLSDVARLADRIAVMVEGRVVACGTVE
                     ELAWQAGVPWDPREPEAPIERIYRNLIQGVPVPVPMTLRLVGA"
     gene            880..1692
                     /locus_tag="VFU75_09425"
     CDS             880..1692
                     /locus_tag="VFU75_09425"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5467100.1"
                     /translation="MTRRALIAFEWAAARRGRVVIFFAIGFALASIGVSLAGLSAGGI
                     LAVQGFSRTSVSLLQLVLWTVPLLALLLGAIQGAECYELEFLAALPVPRARLVVGRWA
                     AWTAALGTALVAGFGAAGVVIGLFAGAADVLRYLALVGIALLLLSAGLAIGLWIGVAA
                     GTRPRAIGVAVVAWFVLAIGLDLTAIALLAVLPAGGATWNLAALLLANPVDSARALAL
                     GIFSADALAGPTGASLRHLLGGWGVLPLTLSLAAWTAGPLHFAGKRFSNKDL"
     gene            1824..2828
                     /locus_tag="VFU75_09430"
     CDS             1824..2828
                     /locus_tag="VFU75_09430"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU5467101.1"
                     /translation="MKARLVVLAVVILGTVAIGAFRNAQPQRVQSVDFAQTPFSVDPT
                     YPATVAPAGDAPVKAFRIPIRDAKVEIAAGVTYAGWTFGGTVPGPAIRVRQGDRVRVT
                     VVNQASMPHSIDFHAARVPMNVDFRMIMPRDSISFEFVARDPGAYLVHCGTPPVLLHL
                     MQGMYLPIIVDPKGGWPGKADKEFVLVQSEFYVQGGDSAQPGQADWQHALDRQASYVV
                     FNGKAFQYQTTPLQVSVGDRVRFFVVNAGPSFTSDFHIVGAIFDRVYPDGDPGHALAG
                     VQTWGVPAGSGAVFETHFAADGSGAGTYAFVTHAFADAAKGAVGLIHVGDGPMVAMAH
                     "
     gene            2829..3248
                     /locus_tag="VFU75_09435"
     CDS             2829..3248
                     /locus_tag="VFU75_09435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467102.1"
                     /translation="MPVLTRWYVKTAILYLVVALLLGAVLAGNGLVPLPAFLLMAGPA
                     QIHLFVVGWLTQLIFGIAYWMFPKATAEQPRGNDALAVLTYVCLNLGLLARVIGEPWQ
                     AVAPGVIAGGLLVASGVLQWLGGVGFAVLAWPRVRGR"
     gene            3262..3663
                     /locus_tag="VFU75_09440"
     CDS             3262..3663
                     /locus_tag="VFU75_09440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467103.1"
                     /translation="MSIWMVRSALVALVLGFSVGAIMLIGKGIGTFGGLSAGIPIHVE
                     LVVLGWMVQLAMGVALWILPRFGARGPARGAAWSWVAWALWNSGIVFVIAGAAGAPTA
                     QLIGRLIELGAAIAFGAAVWSRVRASGISAM"
     gene            complement(3681..>3897)
                     /locus_tag="VFU75_09445"
     CDS             complement(3681..>3897)
                     /locus_tag="VFU75_09445"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEU5467104.1"
                     /translation="PVETLLRELTDWAADLVIVGSHGRNFVMRALLGSVSEELLNQLP
                     TSLLMVPPLRRAGERVRTPTPQLAGAL"
BASE COUNT          516 a         1237 c         1411 g          733 t
ORIGIN      
        1 gtctcggggg cacgcaggcg caagccgctg cgggtgcggc gttgaccttg ggtgcgaccc
       61 tcatcctggt gggatttggg agacgggagg cgacgaggtg aaggatcggt acggcatcga
      121 gctctgtgga gtcgggaagg catatggcag ccgcgtggct ctcagcggtc tcgacctgaa
      181 cgtcgccgcg ggagaagttg tggcccttgt ggggcccaat ggggcgggga aatcgacgac
      241 gctccgcatt ctgtcgggtg ccattcatcc gtcggcggga atcgcgcgga tcggtggacg
      301 ggatgtgagc ctcgaccccc tgggggctcg ggagcggctc gggtacctgc cgcagcggct
      361 gggggtgccc ctgtccacgg tggtgcggga cctcgcggca ctggtctccg ctgctcgcgg
      421 gctccccgcg gacgccggcg cgcgggcact cgcggaagcg catctcggca atcgtctcga
      481 cgcgaccctc ggacagattt cgggagggca gcgccagcgg gtgatgctgg tgctggcgac
      541 gctcggggcg gttgaggtgc tgctcctgga cgaaccgagc atctcgctgg acaccgaggg
      601 tgcggaggag gtccgggatg cgatctcggc agcgcggttc gacggcgcgg cgatcctgtt
      661 cgcgtcgcac cacttgagtg acgtcgcccg gttggccgat cgcatcgcgg tgatggtcga
      721 gggccgggtg gtcgcgtgtg gaacggtcga ggagctcgcc tggcaagcgg gcgttccgtg
      781 ggatccccgc gagcccgagg cgccgatcga gcgcatctac cgcaatctga tccagggcgt
      841 tcccgtgccc gttccgatga ctctcaggct ggtgggcgca tgacgcgccg tgcgctgatc
      901 gcgttcgagt gggcggcggc acgccgcggt cgcgtcgtga tcttcttcgc catcggattc
      961 gcgctggcga gcatcggcgt ttccctggcc ggactttcgg cgggaggcat cctcgccgtg
     1021 cagggcttct cccgcacgtc ggtgagcctg ctgcaactgg tgctgtggac ggtcccgttg
     1081 cttgcccttc tgctgggcgc cattcagggt gccgagtgct acgagctgga gtttctcgcg
     1141 gcgctgccgg tgccacgggc gcgcttggtc gtcggtcggt gggcggcatg gaccgcggcc
     1201 ctgggaacgg ccttggtcgc aggattcggg gctgcgggcg tggtgatcgg cttgttcgcg
     1261 ggagccgcag acgtcctccg gtatctggct ctggtcggga tcgccctctt gctcctgtcc
     1321 gccggcctcg ccatcggcct ctggatcggc gtcgcggcgg gaacccgtcc gcgcgccatc
     1381 ggcgtcgcgg tcgtcgcctg gttcgtgctg gcgatcggcc tcgatctcac cgctatcgcc
     1441 ctgctggccg tgttgcctgc gggaggggcc acctggaacc tcgcggcgct gttgctggcg
     1501 aacccggtcg acagtgcgcg ggctcttgcg ctcggcatct tcagcgccga cgctctggcc
     1561 ggtcccaccg gagcatcgct ccggcacctg ctgggagggt ggggcgtcct ccccctgacg
     1621 ttgagcctcg ccgcttggac cgccggaccg ctgcactttg ccggtaagag gttcagcaac
     1681 aaagacttat agtttcaacg gttgtaccgg ttatttcggt agaaatcttc acgcggggct
     1741 aaagtctcct tcacaccccc cacgcgattc tcttcacgca cggcaggcaa ccagcacaca
     1801 ccaactcgcg tggggagtaa ctaatgaagg ccaggctcgt ggttctcgca gtcgtgatcc
     1861 ttggcacggt ggcaatcggc gcgttccgca acgcccagcc acagcgggtt cagagcgtgg
     1921 atttcgctca gacccccttc tccgtcgatc cgacgtatcc ggccaccgtg gcgcccgcgg
     1981 gggatgctcc cgtgaaggcg ttccggatcc ccattcgtga cgcgaaggtc gaaatcgcgg
     2041 ccggcgtcac gtacgccggt tggacgttcg gcgggacggt tccagggccc gcgattcgcg
     2101 tgcgtcaggg agaccgcgtg cgggtgacgg tcgtgaacca ggcgtcgatg ccgcattcga
     2161 tcgatttcca cgccgcgcgc gtgcccatga acgtcgactt ccgcatgatc atgccccgcg
     2221 actcgatctc gttcgagttc gtggcgcgag atcccggtgc ctatctggtc cactgcggca
     2281 cacccccggt gctgctgcac ctgatgcagg gcatgtacct gccgatcatc gtcgatccga
     2341 agggcggctg gcccggcaag gcggacaagg agttcgtgct ggtacagagc gagttctacg
     2401 tccagggtgg ggacagcgcg cagccgggtc aggccgactg gcagcacgcg ctcgaccggc
     2461 aggccagcta cgtcgttttc aacggcaaag ccttccagta ccagaccaca ccgctccagg
     2521 tcagcgtcgg tgaccgggtc cggttctttg tcgtgaacgc cggtccgagc ttcaccagcg
     2581 acttccacat tgtcggcgcg attttcgacc gggtgtatcc ggacggggat ccgggccacg
     2641 cgctcgccgg cgtccagacc tggggagttc ccgcgggctc cggcgcggta ttcgagacgc
     2701 atttcgcggc cgacggcagc ggagccggga cctacgcgtt cgtgacccac gctttcgccg
     2761 atgccgccaa gggcgcggtg ggattgatcc acgtcggcga cggcccgatg gtcgcgatgg
     2821 cacactgagt gccggtcctt acgcgctggt acgtcaagac ggcgattctg tatctggtag
     2881 tggctttgct cctgggagca gtcctcgcgg ggaacggcct ggtgccgctc cccgcgtttc
     2941 tgctcatggc ggggccggcg cagattcacc tgttcgtggt gggttggcta acgcagctga
     3001 ttttcggcat cgcgtactgg atgtttccca aggccacggc cgagcagcca cgggggaacg
     3061 acgcgctggc ggtgctgacc tacgtctgcc tcaacctcgg cctcctggcc cgggtcatcg
     3121 gagagccctg gcaggcggtg gcgccgggtg tcatcgcggg cgggctcctg gttgcctccg
     3181 gcgtgctcca gtggctcggg ggtgtcggat tcgcggtgct cgcgtggccg cgggtgcggg
     3241 ggcgctgacg ccatgccaag ggtgagcatc tggatggtgc gatcggcgct ggtggccctc
     3301 gtgctgggat tctcggtcgg cgccatcatg ctgattggca agggcatcgg cacgttcggc
     3361 ggattatccg ccggcatccc gattcacgtg gagctggtgg tcctgggctg gatggtacag
     3421 ctcgcgatgg gtgtggcgct ctggatcttg ccccgcttcg gcgctcgcgg cccggcccgt
     3481 ggcgcggcct ggagctgggt cgcctgggct ctctggaaca gcgggatcgt gttcgtcatt
     3541 gccggcgcgg ctggtgctcc cacggcacag ctcatcggac ggctgatcga gctcggtgcg
     3601 gcaatcgcgt tcggcgcggc ggtctggagc cgagtgcgtg cgtcagggat ttcggcaatg
     3661 tgaagatcgg tccgagaccg ctagagcgct ccggccaact gaggcgttgg agtgcggacg
     3721 cgctcacccg cccggcggag tggcgggacc atcaggaggg acgtggggag ctggttcagg
     3781 agctcctcgc tcacgctccc cagcaatgcc cgcatgacga agttccggcc atgcgatccg
     3841 acgatgacca gatccgccgc ccagtcggtc aactcgcgga gcagggtctc caccggg
//
LOCUS       DASUBP010000249         3593 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_949014, whole genome shotgun sequence.
ACCESSION   DASUBP010000249 DASUBP010000000
VERSION     DASUBP010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3593)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3593)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3593
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949014"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VFU75_09450"
     CDS             complement(<1..324)
                     /locus_tag="VFU75_09450"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5467105.1"
                     /translation="MTAFELQPTLTGRLLEMRPLRVDDWDVIYALARDPLIWEQHPDR
                     ERWREERFRVVFREGMESGGALVAIDRQTGRAIGFTRYHDYRPERSEIEIGWTFLARS
                     HWGGKY"
     gene            complement(321..689)
                     /locus_tag="VFU75_09455"
     CDS             complement(321..689)
                     /locus_tag="VFU75_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467106.1"
                     /translation="MRAHAAWPLAYAMLIPAAMQAQRLEPPAVSFRASQALQAPNAPE
                     LIQRGNHGTEGALIGGVTFGVIGYWLAGKGCEIRTYPASSSFNTCNTGTKLVFAGAIA
                     LVGVGLGYLVGTGYPKYVEQ"
     gene            complement(702..3197)
                     /locus_tag="VFU75_09460"
     CDS             complement(702..3197)
                     /locus_tag="VFU75_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467107.1"
                     /translation="MNLSRHRIAVLLAAVGLALSCSDSPIGPGSTRTPVSVGDTVSGS
                     LAAQQYEALYSFHAVQDSFYAAFLQAAIGGIALDVEDSTRSLLLEHMFGGPTPQLLDD
                     PGPAFKLPWTGDFVVSVNDPGTRPGTTFRFLLYQVHTAPELIPPRFAIGDTVSGETLE
                     TSADVDRFVFAGTAGQEVVAYAQAMSGDSGRRLGISINAPGTPAVTSLGGDTSLEMQS
                     TGRFTLPATGDYNLTVLGAPDLLNQHPGFHGPYRFQVHAVNHHPESVPDSLIPGDTVS
                     DERLDYVGDIDEFRVAAPPGQTVNVFIQGNTGNPSDSFVVDLRHPADTGFSQQTMTSG
                     ADPNLLAHATGNFVIPPGSAPLIRVRGEWDRFNVPDRGPYRIFVYPVNAAPEHNPDSL
                     VLNDSVGETIEVPGDIDTFRLTLPQPALVNYVLRRGPSTSTNALQLAISGPDSIVVED
                     PDSFAASGVALRPAGSYVLRMSGQTSKDGGYVGPYELTAWRIDTMPETAAESLAVGDT
                     VTEPLEPLGDRDTYVFHGVNGQHILVSLEALTGVGYQMAVLDPAGALLSTAYVGANTG
                     RIDLTQTGWYHIAIGPNNNGAESIDHGIYRFTIGSVSGAPEHSPDSVVVGDSISTESL
                     DFSGDLDQFRLIGSPNQEVAVFFRTQMYPVTLDVIDTASLQVLASTITSVPTARFKLP
                     ASGSTPLIRVYGPGPANGSQGPYWLWVYPVNRAPESVPATVTLGDTVSGETINPLADV
                     DEFQFAGTSGQVVTVLFDTPTGASIPGYQLQFVDASNDSVLAQVTSGNPSNHFGDVNT
                     GPITLPRTGTYLVRVQAADYQAINNQPYKFLIQ"
     gene            complement(3236..>3593)
                     /locus_tag="VFU75_09465"
     CDS             complement(3236..>3593)
                     /locus_tag="VFU75_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467108.1"
                     /translation="ERSIVGESLAGLFIVETFLVEPTLFRHYIALDPSLWWNDGGLVD
                     AAPSLLAGRDTAPRSLFLASSSQPDIAAGTQHLARLLDAIPPGQLTWVCTPRPDLTHA
                     TIFRGVGPGALAFTLK"
BASE COUNT          653 a         1109 c         1220 g          611 t
ORIGIN      
        1 gtacttcccg ccccagtggg accgggccag gaacgtccag ccgatctcga tctcggatct
       61 ttcggggcga tagtcgtgat agcgggtgaa tccgatggcc ctgccggtct gacggtcgat
      121 cgcgaccagc gcgccccccg attccatccc ttcgcggaag acgacccgga agcgctcctc
      181 gcgccagcgc tcgcggtcgg ggtgctgttc ccagatgagc ggatcccggg ccagggcgta
      241 gatcacatcc cagtcgtcca cccgaagcgg ccgcatctcc aggagtcttc cggtcagggt
      301 gggctgcagc tcgaacgcgg tcactgctcg acgtacttcg gatagccggt gccgacgaga
      361 taacccaggc cgacgccgac cagtgctatg gcgccggcga acaccagttt ggtgccggta
      421 ttgcaggtat tgaaggaaga agatgccggg tacgtcctga tctcacagcc tttacccgcc
      481 agccaatagc cgatgacgcc gaaggtgacg cccccgatga gggcgccttc ggtcccgtga
      541 ttcccccgct gaatgagctc cggcgcattc ggcgcctgca aggcctgaga cgcgcggaat
      601 gagacggccg gaggttccag tcgctgcgcc tgcatcgcgg ccgggatgag catggcgtac
      661 gcgagaggcc atgcggcgtg tgccctcatc ggtcctcctc gctactggat caggaacttg
      721 tagggctgat tgttgatggc ttggtagtcg gctgcctgta cccggacaag gtaggtgccg
      781 gtccgcggga gagtgatcgg ccccgtgttc acgtcgccga agtgattcga cgggtttccg
      841 ctggtgactt gcgccagcac ggaatcgttc gacgcgtcga caaactggag ctggtacccc
      901 gggatggacg cccccgtcgg tgtatcgaag agcacggtga cgacctggcc actcgttccg
      961 gcgaactgga actcgtcgac gtccgcgagc gggttgatgg tctcgccgct gacggtgtcg
     1021 cccaacgtca ccgtggccgg gacggactct ggggcacggt tgaccgggta gacccagagc
     1081 caatagggcc cttgcgaccc gttcgccggg ccgggaccgt acacccggat gagaggcgta
     1141 ctgcccgacg ccgggagctt gaaccgcgcc gtgggaacgg atgtgatggt gctggccaac
     1201 acctggagac tggccgtatc gatgacgtcg agagtcaccg ggtacatctg ggtcctgaag
     1261 aacaccgcca cttcctggtt gggagatccg atcaagcgga actggtcgag atctccgctg
     1321 aagtccaacg actcggtcga aatggaatcg cccaccacta ccgagtccgg cgaatgctcc
     1381 ggcgctccgg acactgaccc aatggtaaac cggtagatcc cgtgatctat ggattccgcc
     1441 ccgttgttgt tgggcccaat cgcgatatgg taccacccgg tctgggtaag atcgattcgc
     1501 cccgtgttcg cgcctacgta cgccgtcgag agcaatgccc cggccgggtc gagtaccgcc
     1561 atctggtatc ccactccggt gagggcctca agggacacga ggatgtgctg gccgttgacg
     1621 ccatggaaga cataggtgtc gcggtcgccc agaggttcca gcggctccgt gaccgtgtcc
     1681 ccgaccgcca ggctttccgc cgcggtctcg ggcatcgtat cgatcctcca cgccgtcagc
     1741 tcgtaggggc cgacgtaccc cccatcctta ctggtctgcc cgctcatccg caggacgtac
     1801 gagcccgcgg gccggagggc cacacccgac gcggcgaagg aatcagggtc ctccaccaca
     1861 atgctgtcgg gacccgagat cgcgagctgg agcgcgttcg tggacgtcga gggaccccgc
     1921 cgcaggacgt agttcacgag agccggctgc gggagggtca gccggaaggt atcgatatcg
     1981 ccgggcacct cgatggtttc gccgacgctg tcgttcaaca cgagcgaatc ggggttgtgc
     2041 tcgggcgcgg cgttcacggg gtagacgaag atccggtatg gcccacggtc cggcacgttg
     2101 aagcggtccc actcgccccg gacccggatc agcggagccg agccgggggg gatgacgaag
     2161 ttgcccgtcg catgtgccag gagattcgga tcggccccgc tggtcatcgt ctgttgagag
     2221 aaccccgtat ctgcgggatg ccgcaggtct acgacgaatg agtcgctcgg attgccggtg
     2281 ttcccttgaa tgaacacgtt gacggtctgc cccggcgggg cggcgacgcg gaattcatcg
     2341 atgtcgccga cgtagtcgag gcgctcgtcg gacaccgtgt caccaggaat gagggagtcc
     2401 ggtaccgact cggggtgatg attcaccgcg tgaacctgga agcggtaggg accgtggaat
     2461 cccgggtgct ggttgagcag atctggagcc ccgaggaccg tgaggttgta gtcgcccgtg
     2521 gccggaagcg tgaagcgccc cgtgctctgc atctcgagcg aggtatcgcc tcccagcgaa
     2581 gtcacggccg gagtacccgg cgcattgata ctgatgccca ggcgtcgccc tgagtccccg
     2641 ctcatcgcct gcgcgtaggc gacgacctct tgtcccgccg ttccggcgaa cacgaaacgg
     2701 tccacgtccg ccgaggtctc gagtgtctcc cctgagacgg tatcgccaat ggcgaaccgg
     2761 ggtggaatca gctccggtgc agtgtgtact tggtacaaca ggaatcgaaa cgtggtcccc
     2821 ggccgcgttc ccggatcatt gacactgacg acgaaatcgc cggtccaggg caacttgaag
     2881 gcaggaccgg ggtcgtcgag cagctggggt gtcgggcccc cgaacatgtg ctccaacagg
     2941 agcgatcgag tggagtcctc gacgtccagg gcgatgccgc cgatcgccgc ttggaggaat
     3001 gccgcataga atgagtcctg gaccgcgtga aacgagtaca gggcttcgta ttgctgggcc
     3061 gcgagtgagc cggagacggt atctcccacg gacaccggtg ttcgagtaga ccctggcccg
     3121 atgggggagt ccgagcagga gagcgcgagg cctaccgcgg cgagaagcac cgcgatgcga
     3181 tgacgagaca agttcatccg gacaaactgc acagagcttg gggggtcgta aaggcctact
     3241 tcagtgtgaa cgccagcgca ccgggcccta ccccgcggaa gatcgtcgca tgcgtgaggt
     3301 ccggcctcgg cgtgcacacc caggtgagct gaccaggtgg aatggcatcc agcaggcgcg
     3361 cgaggtgctg agtgcccgcc gcgatgtcgg gctgagagga gctcgccagg aagagcgacc
     3421 ggggcgccgt gtcacgtccg gcgagcaagg aaggcgccgc atcgaccagg ccaccgtcgt
     3481 tccaccagag cgagggatcg agggcgatgt agtgccggaa gagtgtcggc tcgaccagga
     3541 aggtttcgac gatgaagagt ccggccaacg attcgccgac gatggaccgt tcg
//
LOCUS       DASUBP010000250         2566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_212558, whole genome shotgun sequence.
ACCESSION   DASUBP010000250 DASUBP010000000
VERSION     DASUBP010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2566
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_212558"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(154..1149)
                     /locus_tag="VFU75_09470"
     CDS             complement(154..1149)
                     /locus_tag="VFU75_09470"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HEU5467109.1"
                     /translation="MRTPALSVLTLVLASSVLRAQDTSVSIVYLPGQVDQIPRRLSGP
                     SLEYPPNVLKMGDGERVLIEAVIDTTGHIEPPTFKIVQTADSAMNASVRATMLATIYS
                     PAMLKGHPVRFMGQIWLVLHSPSSPSAVNATALISQARGLPRSSSDSALRLLAEALDT
                     SAHPSDGERAYGLLERGVVETNAGRTESAALDLKRGLDLWRLEHARGIELAPFLNDLA
                     DSIRLAGRGARAVAAGDHLVVLGTADVAPTLLSRPPVVYPPEARALGIAGTVTVEAEV
                     DSSGHVTGTPTVVETPNPLLNGAAVRIVQASRFRPARLGGRSIGVRIRQAISFRP"
     gene            complement(1192..1836)
                     /locus_tag="VFU75_09475"
     CDS             complement(1192..1836)
                     /locus_tag="VFU75_09475"
                     /inference="COORDINATES: protein motif:HMM:NF015506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HEU5467110.1"
                     /translation="MFDRFGVGAPSPFSVHGGILVIAAHVALIAYGLRTTTRREDPPD
                     PVVVAPLALFHQPERRGATGSAPVIPAPGPIDVSTSIPPVVPVTISGGPIEPWPIVRT
                     DSIGSPAAPDLGGVYDPSIVEQQPELLSSPPPRYPEMLRLAHVEGVVIVQAVVDTLGH
                     VERSTLKVISSPHPALSASALECLSGAVFRPGRVEGRAVRVLVQVPVQFTISGR"
     gene            complement(1936..>2566)
                     /locus_tag="VFU75_09480"
     CDS             complement(1936..>2566)
                     /locus_tag="VFU75_09480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5467111.1"
                     /translation="VLRAGVERAVTTPNLPQETLVVLEETLQEVNRMTELVDALLTLA
                     RADEGRAPLAREMVDLRGLVHEVEETGELLAEPGEVRIEIDVPAEPVMLEVDRTRIRQ
                     LVMNLMTNAVKYTPAGGLVKISLAQQPQRVILAVSDTGIGIAPGDLPHIFDRFYRAEF
                     ARTRTGERAGAGLGLAICRWIAEAHGGTIEVQSRPGRGTTFTVTLPRTL"
BASE COUNT          467 a          855 c          853 g          391 t
ORIGIN      
        1 ccatgaagcg gcccttctgc gtcttccgct tcgactcgat cagattatcg aacacgaacc
       61 gtgtctccca tgggaggacc aaggaaatac tacccgccgc cggttacccg ctagtgggca
      121 agacattaca aaacgattaa agcgaaggcg gagtcaggga cggaaggaga tcgcctggcg
      181 gatacgaact ccgatggacc ggcccccgag ccgcgcaggc cggaaccggg acgcctgcac
      241 gatccgcacg gctgctccgt tgaggagcgg attcggagtc tccaccacgg tcggcgttcc
      301 ggtgacatgt ccactcgaat cgacctcggc ttccacggtg acggttccgg caatgccgag
      361 cgcgcgcgcc tcaggtggat acaccactgg aggccgggag agcaacgtgg gagcgacatc
      421 cgcggtcccc agcacgacca aatggtctcc ggcggcgacc gctcgcgcac cacgtccggc
      481 aagccgaatc gagtccgcaa ggtcattcag gaatggcgcg agctcgatcc cgcgggcgtg
      541 ctcgaggcgc cagaggtcga gcccccgctt caaatcgagc gcggctgact cggtccgccc
      601 ggcgttcgtt tccactaccc cgcgttcgag cagcccatag gcacgctcgc cgtcgctcgg
      661 gtgggccgag gtgtccagcg cctccgccag caaccgaagg gcactgtccg acgagctccg
      721 aggaagccca cgcgcctggg aaatcagcgc cgtcgcgttg accgccgagg gagagctggg
      781 gctgtgcagt acgagccaga tctggcccat gaaccggacc gggtggccct tgagcatcgc
      841 cggggagtag atcgtcgcga gcatcgtggc ccgcacagag gcgttcatcg cggaatcggc
      901 cgtctgcacg atcttgaacg tggggggctc gatgtgcccc gtcgtgtcga tgaccgcttc
      961 gatgagtacg cgctccccgt cacccatctt gagcacgttc ggcggatact cgagagaagg
     1021 tccggagagc cgtctcggga tctggtccac ctggcccggc aggtagacaa tgctgaccga
     1081 cgtgtcctgc gcccgaagca cggaggacgc gaggaccagg gtgagtacgc tgagcgcggg
     1141 ggttcgcatt accgctcaaa ctacccaccc cggtctcgac ggcaaggagt tctagcgacc
     1201 cgaaatcgtg aactgcaccg gtacctgcac gagtacgcga acggcccggc cttcgactcg
     1261 tcccggacga aataccgcgc ctgaaaggca ctcgagagcg gaggcggaga gggccggatg
     1321 tgggctcgag atcaccttca gggtgctgcg ctcgacatgc cctagggtat cgaccacagc
     1381 ctgcacgatc acgacgcctt ctacgtgcgc gaggcggagc atctccgggt agcgcggggg
     1441 gggcgacgac aggagctccg gctgctgctc gacgatgctc ggatcgtaga ccccaccgag
     1501 atcgggcgcc gcgggactcc cgatcgaatc ggtcctgacg atcggccagg gctcgatcgg
     1561 tcccccggag atcgtgacgg gaaccacggg ggggatcgag gtgctgacgt cgatcggtcc
     1621 gggcgccggg atgacgggag cagatccggt cgcgccacgt cgctccggct ggtggaaaag
     1681 ggcgagcggc gcgacgacca cggggtcagg cgggtcttcg cgtcgagtcg tcgtgcgcag
     1741 cccgtacgcg atgagagcga cgtgcgcggc aatcaccagg attcccccat ggacgctgaa
     1801 tggcgacggc gcgccaacac cgaaccggtc gaacatcgaa acgctcccga agaggttcga
     1861 gtcgatgcta ccagcgcggc gtggccccct catggggggc acatgacaaa cggataaaag
     1921 ggtggtgagg gacgactaaa gggtccgggg caaggtcacg gtgaaggtcg tgccccgccc
     1981 cggccggctc tgcacctcga tggtcccgcc atgtgcttcg gcgatccacc ggcagatcgc
     2041 gagccccaac ccggcccccg cgcgctctcc cgtgcgggtc cgcgcgaact cggcccggta
     2101 gaaccggtcg aagatgtgcg gaaggtcacc cggcgcgatg ccgattcccg tgtccgaaac
     2161 ggccagaatg acccgttgcg gctgctgcgc gagcgagatc ttcaccagcc cgcccgcggg
     2221 agtgtacttc accgcgttgg tcatcaggtt catcacgagt tgccgaatcc gggtccggtc
     2281 gacttcgagc atcaccggct ccgcgggcac atctatctcg atccggacct cacctggttc
     2341 ggccaggagc tcgcccgtct cctcgacctc gtggaccaac ccgcgcaaat ccaccatctc
     2401 ccgggcgaga ggtgcccgtc cttcgtcggc ccgggcgagc gtgagcagcg catccaccaa
     2461 ctcggtcatc cggttcactt cctgcagcgt ctcttccagg acgaccagcg tttcctgcgg
     2521 aaggttgggc gtggtcaccg cccgctcgac cccggcccgg agcacg
//
LOCUS       DASUBP010000251         5521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_444204, whole genome shotgun sequence.
ACCESSION   DASUBP010000251 DASUBP010000000
VERSION     DASUBP010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5521
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_444204"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..1624
                     /locus_tag="VFU75_09485"
     CDS             170..1624
                     /locus_tag="VFU75_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467112.1"
                     /translation="MKLQTMLQRMPRPRGDGLRLLAISAGLILFSGACNLLDVTNPDV
                     VPTGNLSDPTALPTIRAGAIGDFALAYGGSGAGGSGGTVEGQILATGMLGDELINTET
                     FPDRVQEDARQTDPSSGTFATVFTNLSRARASTEGAAAKFRALSDTTKDPGLSEMTSL
                     AGFTYVLFAEGYCSGVPITNLTPSGTLIYGTPLTTLQLLDTAIARFTVALNAATAAGS
                     ASMTNLARVGLARANLDLGNFAAAGAAAAPVPITFSYVTQYDLNTTRQNNGVYNGTFT
                     FKRYGMADKEGGVGLPYISQPDPRTPIYRPSGNSGLGFDGVTPQFDQLRYWDKKADIP
                     LASGLEARLIQAEAFLQAADTVNFMAELNRPRVDTVPQYILAGNPAAPTTTTPAPINP
                     MSPLATPASPAASIALLFSERARWLWLTGHRLEDMRRLVRVGGGGYGLAANTVWPNGP
                     YFKNGLTYGGDFNLPVPLIEQNNPNFTACLDRNP"
     gene            complement(1680..2117)
                     /locus_tag="VFU75_09490"
     CDS             complement(1680..2117)
                     /locus_tag="VFU75_09490"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEU5467113.1"
                     /translation="MPSPARYLALASLGALAVACHPRPLALGSAPSAGRIVISQAEIQ
                     RSGGLTAWDVLKKELPEFTFHENARGEPTRLERRGQSSILLNDAPMIFLDGARMTDYH
                     TLTQVLASTLQRIEILDGVEATTYYGTDAVDGAILIFSKNGTE"
     gene            2245..3075
                     /locus_tag="VFU75_09495"
     CDS             2245..3075
                     /locus_tag="VFU75_09495"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEU5467114.1"
                     /translation="MTSILLRILLLLQGLTIPAPRGYVSDFAGVLDSASIQKMTAVIE
                     DLKAKTGGEIAVVTLADLQDRAASDVALGIGRQWGVGQKGNPGDPARNRGIVILLVPR
                     KDHRAGSGAFWIATGTGSEGFVTDAAAGRVRDAVMSQLSNEDYGGALVQATELVAQRF
                     ADEFHVALDPSSRPRARFRVIPDGETVNPGAVFAILVIALVIGVTILRGLAGGGRGSL
                     LSWLVLAALQSNRRNRWGGGGAGGGFGGFGGFGGGGGGGFGGFGGGGGFSGGGAGGSF
                     "
     gene            3079..3798
                     /locus_tag="VFU75_09500"
     CDS             3079..3798
                     /locus_tag="VFU75_09500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467115.1"
                     /translation="MANMTVEQFTEGVRSALGARLVSLVVFGSAARRNATDSVDTLVI
                     CDRADSGLLGTLAGVSGAWIKSGHPAPLVFSESEWRDSADAFAIEFLDVKAHHKVLAG
                     RDPWAGITIADEDVRRQLENELRGKVVRLRQAFLGTSGNPRALGQAMAGSVSGFLTML
                     RALLRVSGEEAPAERTALVAAAAARVGFDAGPVTALALAARDRKVLKLNPDDPQVAGY
                     LTAVTRTAEYVNRLPTRSGGR"
     gene            3795..4358
                     /locus_tag="VFU75_09505"
     CDS             3795..4358
                     /locus_tag="VFU75_09505"
                     /inference="COORDINATES: protein motif:HMM:NF015944.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEU5467116.1"
                     /translation="MNARRWTLMLGLTLVAGCGYNTIQTYDEQVNQAGAQIKVQLQRR
                     ADLIPNLVATVKGYAKQEDTIFISVANARAKLAGAVQSGDLSQMATANAALTAPLGRL
                     LAIAENYPQLKSNENFKSLQDEIEGTENRIAVARQDYNQAVNTYNAYIRRFPAVMTAK
                     IIGKGPRPYFDVQTADAATAPKVDFSH"
     gene            complement(4477..5424)
                     /locus_tag="VFU75_09510"
     CDS             complement(4477..5424)
                     /locus_tag="VFU75_09510"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEU5467117.1"
                     /translation="MSQKHQKTDRRWTVMVVPHGSGSSRAVEVSLSVVKALIGFGSVL
                     GLVLVVLGGAALTRGISITRSRALERENRLLSDELQRMRSRLADLQDTLHVFSQRDEE
                     VRLLAGLAPLDSSVRQAGIGGPQGAWSERDSLAALGPAGQQALAVRQDMDGLIRRADI
                     LSKSVGEAYDSLHSHQARFAATPSIMPTKGWLTSAFASERVHPILHLARPHEGIDVAA
                     PMGAEIEAPAAGTVTEVKWEDGYGNFVTIDHGYGLVTRYAHCSKILVVRGQRVKRGQK
                     IALVGATGLATGPHLHYEVWVAGKPVDPLHYVMPDAIVD"
BASE COUNT          980 a         1747 c         1876 g          918 t
ORIGIN      
        1 tcagcatctc gggacgcaat ctgaagacct ggaccaacta caagggtctg gatcctgagc
       61 tgaatgaagc cggacaaagc aacttcacga ccgcggattt cctgactcag ccgccggtgc
      121 ggacctgggt cacgcggatc aatctcacct tctgacgagg cgcggagcca tgaaactgca
      181 gacgatgctt cagcggatgc cgcggccccg tggggacggg ctgcgactgc tcgccatctc
      241 ggcgggcctg attctgttct cgggtgcctg caacctgttg gatgtcacca atcccgacgt
      301 ggtgccgacg ggcaacctga gcgatcctac cgccctgccc accatccggg ccggcgccat
      361 cggcgacttc gctctggctt acggcgggtc gggcgccggt ggttcaggcg ggaccgtgga
      421 gggccagatc ctggccaccg gaatgctcgg ggatgaattg atcaataccg agacgttccc
      481 ggaccgggtg caggaggatg cccggcagac cgacccgtcg agtgggacgt tcgccaccgt
      541 gttcacgaac ctgtcccggg cgcgggcgtc gaccgagggc gccgccgcaa agttccgcgc
      601 gctgtcggat accacgaagg atcccggact gtccgagatg accagtctcg ccgggttcac
      661 ctacgtattg ttcgcggagg gatactgctc gggggtgccg atcaccaacc tgaccccgtc
      721 cggcaccctg atctacggga cgcccctgac gacgttgcag ttgctggata ccgccatcgc
      781 gcgcttcacc gtggcgctca acgcggcgac ggcggcgggc agtgcaagca tgaccaacct
      841 tgcccgggtg ggattggctc gggcgaatct agacctgggg aatttcgcgg cggctggagc
      901 ggcggcggcg ccggtgccga ttacgttctc ctacgtcacc cagtatgact tgaacacgac
      961 ccgtcaaaac aatggcgtct acaacgggac gtttaccttc aaacgttacg gcatggcgga
     1021 caaagaagga ggggtcgggc ttccgtacat ttcgcagcct gatccacgga caccgatcta
     1081 tcggccgagc ggcaactccg ggttgggctt cgacggcgtg acgccgcagt tcgatcaact
     1141 ccggtactgg gacaagaagg cggacatccc gctggcgtcg ggtctggaag cccggttgat
     1201 tcaggccgag gcgtttcttc aggccgcgga cacggtcaac ttcatggccg agttgaaccg
     1261 tccacgcgtg gacacggtgc cccaatacat tctggccggg aacccggcgg ccccgaccac
     1321 cacgacgcct gccccaatca accccatgag ccccctcgcc acgccggcga gtccggcggc
     1381 ctcgatcgcg ttgctgttct ccgaacgcgc caggtggctc tggctcaccg gccaccgcct
     1441 cgaggacatg cggcggttgg tgcgcgtggg tggcggtggg tacggattgg cggcgaatac
     1501 cgtctggccg aacgggccct atttcaagaa cggcctgacc tatggcgggg acttcaacct
     1561 gccggtaccg ctgatcgagc aaaacaaccc caacttcacg gcatgcctcg atcggaatcc
     1621 ctgatcggct gtgaacggaa gaatgcgacg ggggaggcca gctggcctcc cccgttgtct
     1681 tactcggtgc cgttcttgga gaaaatcagg atggcgccgt cgaccgcgtc ggttccgtaa
     1741 tacgtcgtcg cctctacgcc atcgaggatt tcgatccgct ggagcgtcga cgccagaact
     1801 tgcgtcaaag tgtggtagtc ggtcatgcga gcgccgtcca ggaagatcat cggagcgtcg
     1861 ttaagaagga tggagctctg tccccggcgc tcaagccgcg tgggctcccc ccgggcgttc
     1921 tcgtgaaacg tgaattcggg gagctccttc ttgaggacat cccaagccgt gagacccccg
     1981 gagcgctgga tttcagcctg ggaaatgacg attcgtcctg cactgggcgc gctgcccagc
     2041 gccaggggcc ggggatggca ggcgacggcc agggcgccca acgaggcgag ggcgaggtat
     2101 cgggcgggag acggcatggg agtcctcctg aaaacgcggg aaccgcggtt tcactgtgca
     2161 tcttgggatg tccgctggcg agggtcaagc gccgttcacc tcctcggtga cacaaatcgt
     2221 gtcaggatcg cgtagattcg gcgcgtgact tcgatcctgc tccgcatcct cctgctcttg
     2281 caagggctca cgatcccggc cccccgcggg tacgtgtccg atttcgccgg cgtcctcgac
     2341 tcggcatcca tccagaagat gacggccgtc atcgaggacc tgaaagcgaa gaccggcggc
     2401 gagatcgcgg tcgtcactct cgcggacctc caggaccgcg ccgcgagcga cgttgcgctg
     2461 ggcatcggac gtcagtgggg cgtcggacag aaaggcaatc cgggcgatcc ggcgcggaac
     2521 cgcggcatcg tgattctcct ggtaccccgc aaggaccatc gggccggtag cggcgcgttc
     2581 tggatcgcca ccggaacggg atccgagggg ttcgtcactg acgcggcagc gggacgggta
     2641 cgcgatgcgg tgatgtctca gctgtcgaac gaggactacg gcggggcgct cgttcaggcg
     2701 accgagctgg tcgcccaacg gttcgccgac gagttccatg tcgcactcga tccctcgtcc
     2761 agaccgcgcg cgcggtttcg ggtgattccc gatggcgaga cggtcaatcc gggggccgtg
     2821 ttcgcgatcc tcgtaatcgc gctcgtcatc ggcgtgacca tcctgagagg cctcgctggc
     2881 ggcggccgtg ggagtctgct gtcgtggctg gtgcttgccg ccctgcagtc gaatcgccgc
     2941 aatcgttggg gtggtggggg cgcgggcggg gggttcggcg gcttcggagg cttcgggggt
     3001 ggtggcggtg gaggattcgg tggattcgga gggggaggcg ggttctcagg gggcggcgcc
     3061 ggagggagct tctagaaaat ggccaatatg accgtcgaac agttcacgga gggcgtccgg
     3121 agtgccttgg gcgcgcgcct ggtgagtctc gtcgtcttcg gctccgccgc gcgccggaat
     3181 gccaccgatt cggtcgacac cctggtgatc tgcgaccgcg cggactcggg actcctcggc
     3241 acgctggcgg gtgtgagcgg ggcctggatc aagagcggcc atccggcgcc gctggtgttc
     3301 agcgaatccg agtggcgcga ctcggcggat gccttcgcca tcgagttcct cgacgtcaaa
     3361 gcgcatcaca aggtcctggc ggggcgcgac ccctgggccg ggatcaccat cgccgatgag
     3421 gatgtccggc gccagctcga gaacgagctg cggggcaagg tggtccggtt gcggcaggcg
     3481 ttcctcggga cctccggaaa tccccgggcc ttggggcagg cgatggccgg gagcgtctcc
     3541 ggattcctga ccatgctgcg ggcgctgctg cgagtgtccg gagaagaagc gccggccgag
     3601 cggaccgcgt tggtcgcggc ggccgccgcg cgcgtgggct tcgatgcggg gccggtgacg
     3661 gccctcgcgc tggcggcccg tgatcgcaag gtgctgaaat tgaaccccga cgatccgcag
     3721 gtcgcggggt atctcaccgc ggtgacgcgg acggcggagt acgtgaacag actacccaca
     3781 aggagcggtg gacggtgaac gcacgccgat ggacgctgat gctcggcctc acgctggtgg
     3841 ccgggtgtgg atacaacacc attcagacct atgacgagca ggtgaatcag gcgggagccc
     3901 agatcaaggt gcagctgcag cgccgggccg acctcattcc caacctggtc gccacagtga
     3961 agggctacgc caagcaggaa gacacgatct tcatctccgt agccaacgcg cgcgccaagc
     4021 tcgccggcgc ggtgcagagc ggtgacctgt cccagatggc caccgcgaat gcggccctca
     4081 ccgcacccct cggcaggctg ctggcgatcg ccgagaatta cccccagctc aagtcgaacg
     4141 agaacttcaa gtcactgcag gatgagatcg aggggacgga gaaccggatc gccgtggccc
     4201 ggcaggacta caaccaggcc gtcaacacgt acaacgccta catccggcgc tttccggcgg
     4261 tcatgaccgc gaagatcatc gggaaggggc cccggccgta cttcgacgtg caaacggcgg
     4321 atgcggcgac ggcgcccaag gtggatttct cgcactaacg actagacggt tagacggtta
     4381 gacggctaga taggcctaga taagcgaaga ccccgtggag tcactctcca tggggtcttt
     4441 ctctaaccgt ctaaccgtct agccgtctag tagttgtcag tccacaatcg catccggcat
     4501 cacgtagtgc aggggatcaa ccggcttgcc ggcgacccac acttcgtagt ggaggtgcgg
     4561 tccggtggcg aggccggtgg cgccgacgag ggcgatcttc tggccgcgct tcacccgctg
     4621 accccgcacc accagaatct tcgagcagtg cgcgtaccgc gtcaccagac cgtagccgtg
     4681 gtcgatggtg acgaaattgc cgtagccatc ctcccacttc acttcggtga ccgtacccgc
     4741 cgccggggct tcgatctcgg cgcccatcgg agcggccacg tcgatgcctt catgaggacg
     4801 ggcgaggtgg agaatggggt gtacgcgctc gctggcgaac gcgctcgtca gccagccctt
     4861 ggtgggcatg atcgacggcg tcgcggcgaa ccgcgcctga tgcgaatgca gggagtcgta
     4921 cgcctcgccc accgacttcg agaggatgtc ggcacggcgg atcaggccgt ccatgtcctg
     4981 gcggacggcg agcgcctgct gtcccgcggg cccgagggcc gcgagggagt cccgttccga
     5041 ccatgcgccc tgcggtccgc cgatgccggc ctgacggacg ctcgagtcga ggggcgcaag
     5101 cccggcgagg aggcggacct cttcgtcgcg ctgcgagaag acgtggagag tgtcctggag
     5161 atcggcgagg cgcgagcgca tccgctgcag ctcatcggag agcaggcggt tctcacgctc
     5221 cagcgcccgc gaccgggtaa tggaaatgcc ccgtgtcagc gccgcaccac ccagcaccac
     5281 caggaccaag ccgaggacgg acccgaagcc gatcaacgcc ttgacgaccg aaagcgacac
     5341 ctccacggcg cgggatgaac ccgatccgtg tggcaccacc atgacggtcc agcggcgatc
     5401 agtcttctgg tgcttctggc tcactaagaa gtccctaagt gcacaggagg cgcaaaaata
     5461 ggcgcagata caaggctcgt caacacgtac cagccactgt caagccacta tgaaacaagg
     5521 g
//
LOCUS       DASUBP010000252         3828 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1030396, whole genome shotgun sequence.
ACCESSION   DASUBP010000252 DASUBP010000000
VERSION     DASUBP010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3828)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3828)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3828
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030396"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3333
                     /locus_tag="VFU75_09515"
     CDS             <1..3333
                     /locus_tag="VFU75_09515"
                     /EC_number="4.1.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF008907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional oxoglutarate
                     decarboxylase/oxoglutarate dehydrogenase thiamine
                     pyrophosphate-binding subunit/dihydrolipoyllysine-residue
                     succinyltransferase subunit"
                     /protein_id="HEU5467118.1"
                     /translation="PAARLVLNMTESLSVPTATSFRDIAVDVLDARRKTLNGQLAAKN
                     KKVSFTHLIGWAIVQAAREFPVMTHSFHEYDGKPHRLDPQNVSLGLAVDVEKKDGTRA
                     LVVPVIKHAETMDFPTFYATYESLVEKARTNKLMPDDFAGGSITLTNPGTIGTVASVP
                     RLMKGQGSIIATGAIRSIGPAKVMTITSTYDHRVIQGAESGMFLRLLDSLLQGEEDFY
                     GKVMAAAEAFGNEKRETGNVAAAETPIPVSRVTSPASPSVDDLRHVAAAMSLVKAYRN
                     FGHLAARLDPLGAEPRGDPALDPGPLGLTPDVLAGIPTEVLRIYVSGATLAEALPQLK
                     ATYCGTIAYEVEHIGSHEQRLWLRRVIESGEHAKPLTADQQKQLLGHLTKVDALERFL
                     QRAYLGQKRFSIEGVDITVPMLELTNELAAHAGAKKVVMGMAHRGRLNVLAHIVGMPY
                     ETIFEEFEGGPTGDEADPDAVTGDVKYHLGADGDYTLPSGTSIRVILLPNPSHLEAVN
                     PVVEGRARAEQTDRSGTDAATNSETAVPILLHGDAAFAGQGVVAETFNLARLNGYSTG
                     GTIHLITNNQIGFTTMPHEGRSTDYSSDLAKGFDVPIIHVNADDPEACLAAVRLAWMY
                     REKFHGDVVIDVVGYRRHGHNEGDEPAYTQPLMYERIKQTPTVREKYASLLVERGVIT
                     ADAAKAEAEAAYQKLDAVKQGLASATARKHGDDRMTRSTTGEPETAVSAELLTVLNTA
                     LLEVPADFTIHPKLKRQLDRRRDTIVKGGMEWAQAEALAFASLLSEGTPIRLTGQDTE
                     RGTFAQRHLMLHDPANDRRWAPIQHLPQAKAPFELHNSPLSEAACLGFEYGYAVAAPN
                     ALVLWEAQYGDFANGAEVIIDQFLVAGLAKWGQTSRLTMLLPHGYEGGGPEHSSARLE
                     RYLSLGAEGNIRVANPTTPAQYFHLLRRQARHAEVRPLVVMTPKSLLRLPAATSSLAE
                     LTSGAFQPVLDDAEAASRRDHVTRLVLCSGKIYYDLVAAPARAGASQVAVGRVELLYP
                     FPAEAIAALTRKYPNLKEVMWVQEEPRNMGARKFALPKLRDTLSNLTIRDLSRPERSS
                     PAEGYPGPHKVEQDRIVAQAFQ"
     gene            3338..>3828
                     /locus_tag="VFU75_09520"
     CDS             3338..>3828
                     /locus_tag="VFU75_09520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467119.1"
                     /translation="MYAIVTVVRDIVTGAFVLSVLAATSNWLVRTQKISPFSSLGRFL
                     KKSSDPVLTPVETRVVRFGGLPSHAGWWLVVVTAVAGIVLVGAAKAFVNVLYDLRFAA
                     EGGVFGVADYLLNGVIDVLILALVVRFFGSLLGAFQFNRWTRWAYVLTNWMIEPLAKV
                     LPQ"
BASE COUNT          673 a         1325 c         1219 g          611 t
ORIGIN      
        1 cccgcggcgc gcctcgtcct gaacatgacg gagtccctct ccgtcccgac ggcgacgagc
       61 ttccgcgaca tcgcggtcga cgtcctggat gcgcgacgca agacgctcaa cggtcagctc
      121 gccgcgaaga acaagaaggt ctcattcacg catctgatcg gctgggccat cgtccaggcc
      181 gcgcgtgagt tccccgtcat gacccactcg ttccacgagt acgacgggaa gccgcaccgc
      241 ctcgaccccc agaacgtgag cctcggcctc gccgtcgacg tggagaagaa ggacggaacg
      301 cgcgcgctcg tcgtcccggt catcaagcac gcggagacga tggacttccc gaccttctac
      361 gccacgtacg agtccctggt cgagaaggcc cgcaccaaca agctgatgcc cgacgacttc
      421 gcgggcggct ccatcaccct gacgaacccg ggcaccatcg ggaccgtcgc gtcggtcccg
      481 cgcttgatga agggccaggg ctccatcatc gcgacgggcg ccatccgctc catcggcccc
      541 gcgaaggtga tgacgattac aagcacctac gaccaccgcg tcatccaggg tgccgagtcc
      601 gggatgttcc tgcggctgct ggattcgttg ctgcagggtg aggaagattt ttacgggaag
      661 gtgatggcgg cggcggaagc ctttggaaac gagaaacggg aaacgggaaa cgtggccgcc
      721 gcggagacgc cgattcccgt ttcccgtgtc acgtctcccg cctctccatc cgtcgacgac
      781 ctccgccacg tggccgcggc catgtcgctg gtgaaggcgt accggaactt cggccacctc
      841 gccgcccggc tcgacccact cggcgccgaa ccccgcggcg accccgcact cgatcccggc
      901 cccctcggcc tcacacctga cgtgctggcg ggcatcccga ccgaagtgct ccggatctac
      961 gtctccggcg ccaccctggc cgaggcgctg ccgcagctca aggcgaccta ctgcggcacc
     1021 atcgcctatg aggtcgagca catcggcagc cacgaacagc ggctctggct gcggcgcgtc
     1081 atcgagtccg gcgagcatgc caaacccctg acggccgacc agcagaagca actgctgggg
     1141 catctgacca aggtcgacgc gctcgagcgg ttcctccagc gcgcgtacct gggacagaag
     1201 cggttctcca tcgagggcgt ggacatcacc gtgcccatgc tcgagctcac caacgagctc
     1261 gcggcgcacg ccggagcgaa gaaggtcgtg atgggcatgg cccaccgcgg ccggctcaac
     1321 gtcctggccc acatcgtagg gatgccgtac gagacgattt tcgaggagtt cgagggcggg
     1381 cccacgggag acgaagccga tcccgacgcc gtgacgggag acgtgaagta ccacctgggc
     1441 gccgacggcg actacaccct gccctccggc acatccatcc gggtgattct ccttcccaat
     1501 ccctcgcacc tcgaagccgt aaacccggtg gtcgagggcc gggcgcgggc cgagcagacc
     1561 gatcgatcgg gcaccgacgc ggccaccaac agcgaaaccg ccgttcccat cctccttcac
     1621 ggagatgcgg cgttcgccgg ccagggcgtg gtggccgaaa cgttcaacct cgcccggctg
     1681 aacggctatt cgaccggcgg gaccatccat ctgattacga acaatcagat cggattcacc
     1741 accatgccgc acgaggggcg gtccaccgac tactcctcgg atctggccaa gggattcgac
     1801 gtcccgatca tccatgtgaa tgcggacgac cccgaggcct gcctcgccgc cgtacgcctc
     1861 gcctggatgt accgcgagaa attccacggt gacgtggtga tcgacgtcgt cgggtaccgg
     1921 cgccatgggc acaacgaagg ggacgaaccg gcctacactc agcccctgat gtacgagcgg
     1981 atcaagcaga cacccaccgt gcgcgagaaa tacgcgtccc tgctggtgga gcgaggggtc
     2041 atcaccgccg atgccgccaa ggccgaagcc gaggccgcct accagaagct ggatgcggtc
     2101 aaacagggcc tcgcatccgc caccgccagg aagcatgggg acgaccggat gacccgctcg
     2161 accaccggtg agccggaaac ggcggtgtcc gcggagcttc tgaccgtgct caatacggct
     2221 ctgctcgagg tgccggcgga cttcacgatc catcccaagc tcaagcggca gctcgaccgg
     2281 cggcgggata ccatcgtcaa gggcggcatg gagtgggcac aggcggaggc gctcgcgttc
     2341 gcgtctctgc tctcggaagg gacgcccatc cgtctcaccg gccaggacac ggagcggggc
     2401 accttcgccc agcggcatct gatgctccat gatcccgcca atgaccggcg ctgggctccg
     2461 atccagcatc tgccgcaggc gaaggcgccc ttcgagctgc acaactcgcc cctgtcggaa
     2521 gcggcctgtc tcggcttcga gtatggctat gcggtcgctg ctcccaacgc actggtgctc
     2581 tgggaagcgc agtatggtga cttcgccaac ggagccgaag tcatcatcga ccagttcctg
     2641 gtggcgggcc tcgcgaagtg gggccagacc agccggctga ccatgctcct gccccacggc
     2701 tacgaagggg gcgggcccga gcactcgagc gcccggctcg agcgctacct ctccctcggc
     2761 gccgagggga acatccgcgt cgcaaatccg accacgccgg cccagtactt ccatctcctc
     2821 cggcgccagg cccggcacgc ggaagtgcga cccctggtgg tgatgacgcc gaagagcctg
     2881 ctgcggctcc ccgccgccac gtcgagtctg gcggagctga cgagcggagc attccagccg
     2941 gtgctcgacg acgccgaggc cgcgagccgg cgcgaccatg ttacccggct ggtgctgtgc
     3001 agcggcaaga tctactacga cctcgtcgcc gctccggcac gggcgggggc gtcccaggtc
     3061 gcggtgggcc gtgtcgagct gctgtatccg ttcccggctg aggccattgc ggcgctcacc
     3121 aggaagtacc cgaatctcaa agaagtcatg tgggtccagg aggagccacg caacatgggc
     3181 gcgcgcaagt tcgctctgcc caagctgcgg gacacgctgt ccaacctcac catccgcgat
     3241 ctgtcgcggc ccgagcgctc gagccccgcc gagggatatc caggacccca caaggtggaa
     3301 caggaccgga tcgtcgctca ggccttccag tgatctgatg tacgccattg tgacggtggt
     3361 gcgggacatc gtgaccgggg cctttgttct ctcggtgctc gccgcgacgt ccaactggct
     3421 ggtccgtacc cagaagatct cgcccttctc gtccctcggc cgcttcctca agaagtcctc
     3481 cgaccccgtg ctcacccccg tcgaaacccg agtggtccgc ttcggcggat tgccctcgca
     3541 cgcggggtgg tggctggtgg tcgtgaccgc ggtggcggga attgtgctgg tgggagccgc
     3601 gaaggccttc gtcaatgtgc tttacgacct gcggtttgcg gcggaggggg gagtatttgg
     3661 agtagcagac taccttctga acggcgtcat cgacgttctt attcttgcgc tggtcgtgcg
     3721 tttcttcggt tccctgctgg gcgcctttca gttcaaccgg tggacgcggt gggcgtacgt
     3781 gctgacgaac tggatgatcg agcccctcgc gaaagtcctg ccgcagta
//
LOCUS       DASUBP010000253         6523 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_308313, whole genome shotgun sequence.
ACCESSION   DASUBP010000253 DASUBP010000000
VERSION     DASUBP010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6523)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6523)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6523
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308313"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="VFU75_09525"
     CDS             <1..501
                     /locus_tag="VFU75_09525"
                     /inference="COORDINATES: protein motif:HMM:NF015764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5467120.1"
                     /translation="GIQSLGQVGEIFFMLALPFLLRRYGIKNILLGAMAAWGARYLAF
                     AFGNAGSLIALVFLGIILHGICYDFFFVSGQIYTDEKAGPRIRGAAQGFINLVTNGLG
                     YFIGSFVSGKTADAFSHCADATSPCVNVVRDWHGIWLVPAACAFLILIGFAIAFRPRL
                     QASPAT"
     gene            511..1854
                     /locus_tag="VFU75_09530"
     CDS             511..1854
                     /locus_tag="VFU75_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5467121.1"
                     /translation="MSKRKGISRRRFVGDATAAGLSFTIVPRHVLGRGYLAPSDKLNV
                     GCVGGGGRAKDDIAGVSATENIYAIADPDWDNAGESFAKFPQAKKYKDYREMLDKEKS
                     IDAVVVACPDHSHTPAALMALRLGKHVYCEKPLCRTLGEVRTLMKAAAERPKLATQMG
                     NQGHAGDGIRLIREWVEAGLIGTVREVHFWTDRPIWPQAIDRPLQEYYVPATLDWNLW
                     LGPAPERPYNPAYEPFNWRGWWDFGTGALGDMACHIMDAAFWTLDLGSPTRVIAETTE
                     QFKETAPKSSRVEYHFAAKGSRAPVTCIWRDGSLYPPRPAEVPVGLPWPPSEDGGSYW
                     IGDKGKLIAGTYGGNPQLLDPQAMAEVTAHPVPQKYPRVTSVYAEWINAAKGNGKTGS
                     SFDGYAGPLTSMVLLGDLAVRMAGAIDVNPATGDVTTSGIPAEWVTPVYRSGWSM"
     gene            complement(1994..3307)
                     /locus_tag="VFU75_09535"
     CDS             complement(1994..3307)
                     /locus_tag="VFU75_09535"
                     /inference="COORDINATES: protein motif:HMM:NF013300.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEU5467122.1"
                     /translation="MDRRRFLQTSGALSATTILPRPGLRPLPAPSSRMDWWRDSRFGM
                     FIHWGLYSILAGKWGGRDDYAEWIRNLAHIPVGEYDKLVGRFNPTLFDADKWIGMAKD
                     AGAKYVTITTKHHDGFCLFDSKLTDFCVRSTPFKRDIMAEMAAASRRAGVHQCWYHSI
                     MDWHHPDYLPRRDWEAETRPVGDAHFSRYVEYLHGQVTELLTKYGDIGVMWFDGQWEG
                     TWTHELGKALYAKCRSLQPNVIVNNRVEGWSGDKITDPLGDFRTPEQEVPATGWPGVD
                     WESCITMNDNWGYNAQDHHFKSAEELIGLLVETASKGGNLLLNVGPKADGTFPDESVE
                     RLAAIGKWMRVNGTAIYGSTASPFPKTPFRVTSQPRKLNLFMKEWAATAELPGLKTPI
                     ARAYLLADSTRAPLPVAENVVTLPATPGGQWPVVVTEFDSQPIVG"
     gene            complement(3313..5886)
                     /locus_tag="VFU75_09540"
     CDS             complement(3313..5886)
                     /locus_tag="VFU75_09540"
                     /inference="COORDINATES: protein motif:HMM:NF019334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU5467123.1"
                     /translation="MIDALRRELAGRYTLERELGRGGMADVFQATDLRHQRRVAFKVL
                     RPEVAQAIGRERFQREVATAARLAHPNILPLFDSGEAGGLLYYVMPLVEGESLRARLE
                     RETQLPIEDAVGIAREVAEALAYAHSRGVVHRDVKPENILLESGHAMLADFGVSSAIG
                     GETRRLTDSGIAVGTPTYMSPEQGAAERQLDGRSDIYSLGCVLYEMLAGEPPFTGPTV
                     QAIVARRFTGDIPRLSTVRPATPQAVEDAVTRALALVPADRWPTGHAFAAALAGEREP
                     SARWRRPRVRLPVVGALVLVLAAGYAAFRLLHRAGVVAPRLAVLPFENLGAMGDQYFA
                     DGVSDEVRGKLTLVPGLTVIARASSVQYRGTATTPAQIGRELGATYLLTGTVRWEKHA
                     DGTSRVHVSPELIRVADGAAQWEQSYEAPLTDVFAVQTGIATQVVQALDVALEAPAQR
                     RLAARPTENLAAYDAYLQGEEASDRLGAVDPSSTRRAIVLYAQAVKLDSTFALAWSRL
                     GAAHALHYTYNDVDSAERTAALTAARRAVGLAPSLPEAHIALGTCESLVAGDQLSALG
                     EYDRAAALMPVPTADVWNGRALAESFLGRWAEAVADLRRALSVDPRSVLTYVRLTRAL
                     LWLREYPEALAVSDSAKAIAPANVILVVHRAMIMVARGDLPGARRVLRDVPASIDRDR
                     LVAQGAQAWGLYWVLDEEDQQRLLATTPTAFDNDRGEWNLTHAEILALQGNASASRAF
                     AESARVAIEVQAGGVPREGSDLIDHALALAYAGRAAEGVREGERAITRPELQDQFAGL
                     DAQFRLARIYLLAGDPDRALDRLTHLLQVPFYISPAWLRLDPDFAALRGNPRFERLTH
                     G"
     gene            complement(5903..>6523)
                     /locus_tag="VFU75_09545"
     CDS             complement(5903..>6523)
                     /locus_tag="VFU75_09545"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEU5467124.1"
                     /translation="TILFQGDSITDCGRDRKVTDPNSAAALGNGYPLLIASQLLATRA
                     EAGYRIFNRGVSGNTVPDCDARWQADTLDLAPDVLSFLIGVNDYWHTLNGSYKGTADN
                     YRQQFTALLARTRTALPKVRFVVLEPFTLRTGVITDSWFPAFDERRKIARQVADNAGA
                     TFLSTQDLFDRLSREAPPAYWAADGVHPTAAGHGAIADMWLEAVGL"
BASE COUNT         1070 a         2212 c         2176 g         1065 t
ORIGIN      
        1 ggcatccaga gcctcggcca ggtcggcgaa atcttcttca tgctcgcgct tccctttctg
       61 ctgcgccgct acggcatcaa gaacattctc ctgggcgcca tggcggcgtg gggcgcccgc
      121 tatctcgcct ttgcattcgg aaacgccggc tccctcatcg cgctggtgtt cctcggcatt
      181 attctccatg gaatctgcta cgacttcttt ttcgtctcgg ggcagatcta tacggatgag
      241 aaggcgggcc cccggattcg cggggcggcg cagggtttca tcaacctcgt caccaacggc
      301 ctcggttact tcatcgggtc cttcgtgtcg gggaagacgg cggacgcgtt cagtcattgt
      361 gccgatgcca ccagcccgtg cgtgaatgtc gtgcgcgatt ggcacggcat ctggctggtg
      421 ccggcggcct gtgccttcct cattctgatc ggattcgcca tcgccttccg gccgcgcctc
      481 caggcgtcgc cggccacttg aggagtcgtc atgtccaagc gtaagggaat ctcccgccgc
      541 cgcttcgtcg gcgacgccac tgccgccgga ctgtcgttca ccatcgtccc ccgacacgtc
      601 ctcgggcgcg gctacctggc accgtccgac aaactgaacg tcggctgcgt cggcggcgga
      661 ggccgcgcga aggacgacat cgccggcgtc tcggccaccg agaacatcta cgcgatcgcc
      721 gaccccgact gggacaacgc cggcgagtcg ttcgcgaagt tcccgcaggc gaagaagtac
      781 aaggactacc gggagatgct cgacaaggaa aagagcatcg acgcggtggt cgtcgcctgc
      841 cccgaccaca gccacacccc tgccgccctg atggccctgc ggctcgggaa acacgtctac
      901 tgcgagaagc cgctgtgccg gaccctcggc gaagtccgca cgctcatgaa agccgccgcc
      961 gaacggccga agttggcgac ccagatgggg aatcaggggc atgcgggcga tggaatccgg
     1021 ttgattcgcg agtgggtgga ggcgggattg atcggtacgg tccgcgaggt gcatttctgg
     1081 accgaccggc ccatctggcc ccaggccatc gaccggcccc tccaggagta ctacgtcccc
     1141 gccaccctcg actggaatct ctggctgggc ccggccccgg agcgccccta caacccggcg
     1201 tacgaaccgt tcaactggcg tggatggtgg gatttcggca ccggcgcgct gggcgacatg
     1261 gcctgccaca tcatggatgc cgccttctgg accctggatc tcggatcgcc cacacgcgtg
     1321 atcgcggaga ccaccgagca gttcaaggaa acggcgccga agagctcacg cgtcgagtat
     1381 cacttcgccg ccaagggctc gcgcgccccg gtgacctgca tctggcggga cggctccttg
     1441 tatccgccac ggcccgccga agtccccgtc ggcctcccct ggcctccgtc cgaggatggc
     1501 gggtcgtact ggatcggcga caagggcaag ctcatcgcgg gcacgtacgg tggaaacccg
     1561 cagttgctcg atccgcaggc gatggcggag gtgaccgcgc acccggtgcc gcagaaatat
     1621 ccgcgcgtca cgtcggtcta cgccgagtgg atcaatgccg ccaaggggaa tgggaagaca
     1681 ggctcgagct tcgacggcta cgcggggccg ctgacgtcga tggtattgct gggagacctc
     1741 gcggtgcgga tggcgggtgc gatcgacgtg aatccggcga cgggggacgt cacgacgtcg
     1801 gggattccgg cggaatgggt gacgcccgtg taccggtctg gttggagcat gtagttgatg
     1861 tacctaaccc cgggcgcgag cccgggggtt acgcgataca ggagctacgc ggctccacaa
     1921 ccccgggggt acgcgataac ggggctacgc tgtgtgatga ccccgggctc gcgcccgggg
     1981 ttatgaacga ccactaccca acaatcggct gcgaatcgaa ctccgtcacc acaaccggcc
     2041 attggccgcc aggcgttgcg ggaagcgtga ccacgttctc cgccacaggg agtggagctc
     2101 gagttgagtc tgcgagcagg tatgctcgcg caatgggagt cttcaggccc ggcaactcag
     2161 cggtcgctgc ccactccttc atgaacaggt tgagctttct cggctgcgag gtcacccgaa
     2221 agggagtctt cgggaacggt gacgccgtcg agccatagat cgccgtcccg ttcacccgca
     2281 tccatttgcc aatcgccgcc agccgctcga cgctctcatc cgggaaggtc ccgtccgcct
     2341 tcgggccgac gttgagcagg agattcccgc ccttcgacgc ggtctccacc aggagcccga
     2401 tgagctcctc ggcgctcttg aaatggtggt cctgcgcgtt gtagccccag ttgtcgttca
     2461 tggtgatgca cgactcccag tccaccccgg gccagccggt ggcgggcact tcctgttccg
     2521 gcgtgcggaa gtcgccgagg gggtcggtga tcttgtcgcc ggaccagcct tcgacccggt
     2581 tgttcacgat cacattgggc tgaaggctcc ggcacttcgc gtagagcgcc ttgccgagct
     2641 catgcgtcca cgtgccctcc cactggccgt cgaaccacat cacgccgatg tcgccgtact
     2701 tggtgaggag ctcggtgacc tgcccgtgca ggtactcgac atagcgggag aagtgggcgt
     2761 cgcccacggg ccgcgtctcc gcctcccaat cgcggcgcgg caggtagtcg gggtggtgcc
     2821 agtccatgat cgagtggtac cagcactggt gaactcccgc tcgccggctc gcggccgcca
     2881 tctcggccat gatgtcccgc ttgaacggcg tcgagcggac acagaagtcc gtgagcttgg
     2941 aatcgaagag gcagaagccg tcgtggtgct tggtggtgat ggtgacgtac ttcgcgccgg
     3001 catccttcgc catgccgatc catttgtcgg catcgaacag cgtcgggttg aagcggccga
     3061 ccagcttgtc gtactcgccg acggggatgt gcgcgagatt gcggatccac tcggcgtagt
     3121 catcgcgccc gccccacttg cccgccagaa tcgagtacag cccccagtgg atgaacatgc
     3181 cgaagcgtga gtcacgccac caatccatcc gggaggaggg agcggggagt ggtcgcagtc
     3241 cgggacgggg aaggatcgtg gtggcgctca acgcgccgct cgtctggaga aaccgtcgtc
     3301 ggtccattgg gatcacccgt gggtcaagcg ttcgaacctg ggattgcccc gcaaggccgc
     3361 gaagtcgggg tcgagacgga gccaggcggg cgagatgtaa aacggcacct gcaggagatg
     3421 cgtcaggcgg tcgagggccc ggtcggggtc gcccgccagg agataaatgc gcgccagtcg
     3481 aaactgcgcg tcgagacccg cgaactgatc ctggagctcg ggccgggtga tggcgcgctc
     3541 cccttcccgg acgccctcag ccgcgcgccc cgcgtacgcc agcgcgagag catgatcgat
     3601 gagatcgctc ccctcgcgtg gcacgccccc cgcctgcact tcgatggcga cacgggcact
     3661 ctccgcgaag gcgcgtgagg ccgaggcgtt cccctggagc gccaggatct ccgcatgagt
     3721 gaggttccat tcaccgcggt cattgtcgaa tgccgtgggc gtggtcgcca gcaggcgttg
     3781 ctgatcttcc tcgtcgagca cccagtacag accccacgcc tgggccccct gggcgacgag
     3841 ccgatcgcga tcgatggagg ccggcacgtc ccgcaacacc cggcgggcac ccggcaggtc
     3901 cccccgggcc accatgatca tggcgcggtg aacgaccaga atcacgttgg cgggtgcgat
     3961 ggctttcgcg ctgtcgctca ccgccagcgc ctcgggatac tcccggagcc acaacagagc
     4021 ccgcgtcagc cgtacgtacg tcaggacgga gcgcgggtcc acgctcagcg cgcggcgcag
     4081 atcggcgacc gcctccgccc agcgccccag aaacgactcg gcgagtgccc gcccgttcca
     4141 gacgtcggcg gtggggaccg gcatcagcgc ggcagcccgg tcgtattcac cgagagcgga
     4201 aagctggtcg ccggcgacca ggctttcgca ggtcccgagt gcgatgtgcg cctcagggag
     4261 actgggggcc agacccacgg cgcgccgcgc ggcggtcagc gccgccgtcc gttcggccga
     4321 gtcgacgtca ttgtaggtgt agtggagtgc atgggccgca ccgagccgcg accaggccag
     4381 ggcaaaggtc gagtccagct tcacggcctg cgcgtagagc acgatcgccc gccgcgtgct
     4441 cgaggggtcg acggcgccca gccggtccga cgcttcctct ccctgcaggt acgcatcgta
     4501 ggccgcgagg ttctcggtcg gccgggccgc gagccgccgc tgcgcaggtg cctccagtgc
     4561 cacgtccagc gcctgcacca cctgcgtcgc gatgccggtc tgaacggcaa acacatccgt
     4621 caggggcgcc tcgtaggact gttcccactg cgccgccccg tccgcgaccc ggatgagctc
     4681 ggggctcacg tgcacccggc tggtaccgtc cgcatgcttt tcccagcgaa cggtcccggt
     4741 caggagatag gtggcaccga gttcgcggcc gatctgcgcc ggtgtcgtcg cggttccccg
     4801 gtactgcacc gaactggccc gtgcgatgac cgtgaggccc ggcacgagcg tcagcttgcc
     4861 ccgcacttcg tcgctcaccc cgtccgcgaa gtattggtcg cccatcgcac ccaggttctc
     4921 gaagggcaac accgcgaggc ggggggcgac cacccctgcc cggtgcaaca gcctgaaggc
     4981 cgcataaccg gccgccagaa ccagcaccag agctccgacg acgggcaggc gcactcgcgg
     5041 ccgccgccac ctggccgacg gctccctctc ccctgccagg gcagccgcga atgcgtggcc
     5101 cgtcggccaa cgatcagcgg gcaccagggc caaggcgcgg gtcaccgcat cctcgaccgc
     5161 ctgtggggtg gccggccgga ccgtggagag gcgcgggata tcgcccgtga accgacgggc
     5221 cacgatggcc tgaacggttg gacccgtgaa gggcggctcg ccggccagca tttcgtacag
     5281 gacgcatccg agcgaataga tgtcactgcg gccgtcgagc tgccgttccg cggccccctg
     5341 ttcgggactc atgtacgtcg gcgtgccgac cgcgattccc gagtcggtga gccgccgcgt
     5401 ttcgccgccg atggcgctgg agactccgaa gtcggcgagc atcgcgtgac ccgactcgag
     5461 caggatgttt tcgggtttga cgtcgcggtg caccacgccg cggctgtgcg cgtacgccag
     5521 ggcctccgct acctctctgg cgatgccgac ggcatcctcg atcggcagtt gcgtttcccg
     5581 ctccagccgg gctcggagcg attccccctc cacgagcggc atgacgtaat agaggagacc
     5641 gcccgcttct ccggagtcga acagcggcag gatgttcggg tgagccaggc gggccgccgt
     5701 ggcgacttcc cgctggaaac gctcgcgacc gatggcttga gcgacctcgg ggcggagcac
     5761 cttgaaggcg acccgccgct ggtgccgcag atcggtcgcc tggaacacat cggccatgcc
     5821 gccgcgcccg agttcacgct ccagcgtgta ccgcccggca agctcgcggc gcagtgcgtc
     5881 aatcacgagc ctgccaatcg cttcatagac caactgcctc caaccacatg tcggcgatcg
     5941 cgccatggcc ggcggcggtc ggatggacgc cgtccgctgc ccagtacgcc ggaggggcct
     6001 cacgcgacag gcggtcgaac aggtcctgtg tcgacaggaa ggtggcccca gcgttgtcgg
     6061 cgacctggcg cgcgatcttc cgacgctcgt cgaaggccgg aaaccacgag tcggtgatga
     6121 caccggtgcg aagggtaaag ggctcgagca cgacgaatcg aaccttgggc aatgccgtcc
     6181 gcgtgcgcgc caggagcgcc gtgaactgct gccggtagtt gtcggccgtg cccttgtagc
     6241 tcccattcaa cgtgtgccaa taatcgttca caccgatgag gaagctcagc acgtcgggcg
     6301 cgaggtccag cgtgtccgcc tgccatcgcg catcacaatc gggcacggtg ttcccgctca
     6361 cgccacggtt gaagatccgg tacccggcct cggcacgggt ggcgagaagc tgcgacgcga
     6421 tcagcagggg gtacccgttg ccgagcgcag ccgcggaatt ggggtcggtc accttccggt
     6481 cacgtccgca gtcggtgatc gaatctccct ggaagaggat ggt
//
LOCUS       DASUBP010000254         5146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1030551, whole genome shotgun sequence.
ACCESSION   DASUBP010000254 DASUBP010000000
VERSION     DASUBP010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1030551"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1369)
                     /locus_tag="VFU75_09550"
     CDS             complement(<1..1369)
                     /locus_tag="VFU75_09550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HEU5467125.1"
                     /translation="MATAVAAVSGGLSAYETRRYRFRYLIAVTVSMATVLELLDTSIV
                     NVAIPHMMGNLGATLDEIAWVSTGYIVANVIVLPITGWLSAYFGRRRYFASSIAIFTI
                     ASFLCGNSHSLTELIVWRIIQGMGGGALLSTSQAILYEEFPKSEYGTAMAIFGIGVMV
                     GPALGPTLGGYIVDAYGWPWIFYINIPIGLLAFFMTLSLIRDSEHAIKVEKIDYWGLL
                     LLAAGIGTLQTMLERGERQDWFESRQIIAFAVISAVSLVVFVWHELTTDHPVVDLRIA
                     KSKQLTAGVLFGMGLGICLYATVFVIPVYMQSLLGMTAEETGWVLLPSAIASGVTMGI
                     FGRSKLNPRVSITIGTLVFGWVMWKHAHFTIQSGTGDFFWPFIFRGFGLGMIFVPLTN
                     LAMAELPMSKIAQGTGLFNLMRQLGGSVGIALSATLLQRFTAQNRAAMAPDISIYNPV
                     FQQRLQ"
     gene            complement(1382..2383)
                     /locus_tag="VFU75_09555"
     CDS             complement(1382..2383)
                     /locus_tag="VFU75_09555"
                     /inference="COORDINATES: protein motif:HMM:NF012739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEU5467126.1"
                     /translation="MREGASITQAPEKPAGGRKRIILIVVGLGLAFMLFTGARRFIFN
                     LSHVTSDNAQVDGHVVPILPKVGGFVASVRVQENTQVKAGDVLVSLDDKDYRARLQQT
                     DADLAVALASVSSPARVGQAEAQVNQAQANAEKAHQDLDRIRPLAAQGIVSSQQLDAA
                     EAAARSADAGLAAAQAALAGADARVAAARASRDQAALQLSYTQVVAPVSGVVSRKNVE
                     VGQLVQPGEPLMSVVPMDDIWITANLKETEVADVKPGDPVDITADAYGGKHFTGKVES
                     ISPATGARFSLLPPDNATGNFTKVVQRVPVRIAVPQSTDPAFTLRPGMSVVVVIKTK"
     gene            complement(2453..3781)
                     /locus_tag="VFU75_09560"
     CDS             complement(2453..3781)
                     /locus_tag="VFU75_09560"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU5467127.1"
                     /translation="MRNRWMLVLGVLFAPRAVAAQTQLKLSFGDAVRMATGQAAPVQL
                     SGLETDAARARVRQTRSALLPGLTAGGSWVNRDYDLDALGLPFPKGGPIQLPTVVPPF
                     STYDARLTFNQTLLDFSSIKRVRASEAQVQSTVAAGDAATESAAQVAALAYLRAVRAQ
                     ASVAARQADSAIAAEVLSLAQAQLQAGVSGTIDVTRAETQVAVAAGALIVARNQFDQA
                     RIALARALGVDPVKTVLTLDDTLSSALGAASVPVTQEDAVNQALNARPELKVEEARGT
                     VAQVSKSAIQSEVLPRIGFEADYGQSGLTLGSSRSTRQFAVAVSIPLLDGFRREGRAA
                     EQDAVIQESQVRQHDLRQQIAADVAGALLDLHSAEAQEGIASQQLSLAADVLSQAEKR
                     FKAGVAGNIEVIDAQATMVKARDAVIDARYGAAAARVALARAAGAARTLH"
     gene            complement(3774..4415)
                     /locus_tag="VFU75_09565"
     CDS             complement(3774..4415)
                     /locus_tag="VFU75_09565"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5467128.1"
                     /translation="MKPRWQRRKEARPEEIVSAALQVFAQHGFAAARLDDVAKEAGVT
                     KGTIYLYFENKEALFQEVIRQTVVPILAAAEQEVRDWKGSSAELLRHMVRGWWATVGK
                     GSLAGVVKLIVAEATNFPELTRFYYKEVIRRGHLLFAQVLQRGVDSGEFRQVDVMVTV
                     RLVIAPMLMAVVHSQSLYRCIREPFDGDRYVEAHLDMLLRGLAPAPIGEWNHA"
     gene            4530..>5146
                     /locus_tag="VFU75_09570"
     CDS             4530..>5146
                     /locus_tag="VFU75_09570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467129.1"
                     /translation="MTSPAQAYSRPIRAIAVVVAIALALRQYLSLSVSPPAYGRLLAV
                     RIGTLVLAIGIAWLATPGRSVRTLRVYAMVLGLSLALAGCLSGLILPTRFWEVAGLLP
                     AVVLGVSLFIPWPWPWQATFATSAFLIECLTLRWIPDAALPTPLADEGALVLLFSAGI
                     ASTIGSRIADNERRRVAESEARFRALFEGAGDPIAALEPDGRVHDA"
BASE COUNT          838 a         1781 c         1663 g          864 t
ORIGIN      
        1 cctgcagccg ctgctggaac accgggttgt agatgctgat gtcgggagcc atcgcggcgc
       61 ggttctgcgc ggtgaagcgc tgcagcagcg tcgccgacag ggcgatcccc acggagccgc
      121 ccagctgccg catcagattg aagagccccg tcccctgcgc gatcttgctc atcggcagct
      181 cggccatcgc gaggttggtc agcggcacga agatcatccc cagcccgaat ccccggaaga
      241 tgaagggcca gaagaagtcg ccggtgccgc tctgaatggt gaagtgggcg tgcttccaca
      301 tcacccagcc gaacaccagg gtgccgatgg tgatggagac ccgcgggttc agtttggagc
      361 ggccgaaaat ccccatggtc acgcctgacg cgatcgcgct gggcagcagc acccatccgg
      421 tctcctcggc cgtcatgccc agcaggcttt gcatgtagac ggggatcacg aagacggtgg
      481 catagagaca gatgccgagc cccatgccga acagcacgcc ggccgtgagc tgtttgctct
      541 tggctattcg caggtcgacg acaggatggt ccgtcgtcaa ctcatgccag acgaacacga
      601 ccagcgaaac ggcactgatc accgcgaagg cgatgatctg ccgcgactcg aaccaatcct
      661 gccgctcccc ccgttcgagc atggtctgga gcgtcccgat gccggcggcc aggagcagca
      721 atccccaata gtcgatcttc tcgaccttga tcgcgtgctc ggaatcccgg atcagggaca
      781 gcgtcatgaa gaacgccaga agcccgatcg ggatgttgat gtagaaaatc cacggccatc
      841 cgtaggcgtc cacgatgtag ccgccgagcg tgggacccag ggccgggccg accatcacgc
      901 cgatgccgaa aatggccatg gcggtgccgt actcggattt cgggaactcc tcgtacagaa
      961 tggcctggga cgtggagagc agggcgccgc ctcccatgcc ctggatgatc cgccagacga
     1021 tcaactcggt gagcgagtgc gaattcccgc acaggaacga cgcgatggtg aagatcgcga
     1081 tggagctcgc gaagtaccgc cggcgcccga aatacgccga caaccacccg gtgatcggca
     1141 gcacgatgac gttggcgacg atgtaacccg tcgagaccca ggcgatctcg tccagcgtag
     1201 cacccaggtt ccccatcatg tgcgggatcg cgacgttcac gatgctggtg tcgagcagct
     1261 ccagcaccgt ggccatcgac accgtcaccg cgatgagata gcggaaccgg tagcgccggg
     1321 tctcataggc ggacaggccg ccagagacgg cggcgactgc ggtggccacg aagaacgctc
     1381 cttacttcgt cttgatgacc acgacgacgc tcatcccggg acgcagggtg aacgcgggat
     1441 cagtgctctg gggaacggcg atccggaccg gcacccgctg gactaccttc gtgaagttcc
     1501 ccgtcgcgtt gtcgggaggc agcaaggaga aacgcgcgcc cgtggccgga gagatgcttt
     1561 ccaccttccc ggtgaagtgc ttgcccccgt aggcgtcggc ggtgatgtcc acgggatccc
     1621 cgggcttcac gtcggcgact tccgtctcct tcagattcgc ggtgatccag atgtcgtcca
     1681 tcgggaccac gctcatcagg ggctcaccgg gctgcaccag ctggccgact tcgacgttct
     1741 tcctgctgac gacgccggag acgggggcca cgacctgcgt gtacgacagc tgcagggccg
     1801 cctgatcgcg cgaggcgcgg gcggccgcca cccgggcatc ggcgcccgcc aaggccgcct
     1861 gcgccgcggc gaggccggcg tccgccgagc gggccgccgc ctcagcggcg tcgagctgct
     1921 gcgaggagac gatgccctgc gccgcgaggg gcctgatccg gtccaggtcc tgatgagcct
     1981 tttccgcgtt ggcctgcgcc tggttcacct gcgcctcggc ctgtccgacc cgggccggcg
     2041 acgagacgct ggcgagcgcg accgccaggt cggcatccgt ctgctgcagc cgggcccggt
     2101 agtccttgtc gtccagcgag accaggacat cgccggcctt cacctgggtg ttctcctgga
     2161 cccgcacgga ggccacgaag ccgccgacct tgggcaggat cggcacgacg tggccgtcca
     2221 cctgggcgtt gtcgctcgtc acgtggctga ggttgaagat gaagcgccgc gcgcccgtga
     2281 agagcatgaa ggcgaggccc agtcccacga cgatcaggat gatgcgcttc cggcccccgg
     2341 cgggcttttc cggcgcctgg gtgatggacg cgccctccct cacttggggg gcttcgctgt
     2401 cgttcgtgga cgtcgagttt cgttctacag tcgttgccat cgtctgtccc ttttaatgaa
     2461 gcgtccgtgc cgcgcccgcc gcccgggcga gtgccacccg cgccgccgcc gcgccatacc
     2521 gcgcgtcgat cacggcgtcc cgcgccttca ccatcgttgc ctgcgcgtcg atcacttcga
     2581 tgttccccgc cacgccggcc ttgaaccgct tttccgcctg cgacaacaca tcggccgcca
     2641 gcgacagctg ctgcgaggcg atcccttcct gcgcctcggc cgaatggagg tcgaggagcg
     2701 cgcccgccac gtcggcggcg atctgctgcc ggagatcgtg ctggcgcacc tgcgactcct
     2761 gaatcaccgc gtcctgctcc gcggcccggc cctcacgacg gaagccgtcc agcaggggga
     2821 tactcacggc caccgcgaac tgtcgggtgc tccgggagga ccccagcgtg aggccgctct
     2881 gtccgtagtc ggcctcgaac ccgatccgcg gcaacacctc gctctggatc gcggatttcg
     2941 agacctgcgc gaccgttcca cgggcctctt ccaccttcag ttcaggccgg gcgttcagcg
     3001 cctgattgac ggcgtcctcc tgcgtgaccg gcacactcgc cgcccccagg gcggaactca
     3061 gggtgtcgtc tagcgtgagg acggtcttta ccggatcgac cccaagggct cgcgccagcg
     3121 cgatccgggc ctggtcgaat tggttgcggg ccacgatgag cgcccccgcc gccacggcga
     3181 cctgggtctc cgcccgcgtc acgtcgatcg tcccggacac accggcctgc aactgggcct
     3241 gagccagcga caggacttcc gccgcgatgg ccgaatccgc ctggcgcgcc gccaccgaag
     3301 cctgggcccg gactgcccgc aggtacgcga gggcggccac ctgcgccgcg ctttcggtcg
     3361 ctgcgtctcc agccgccacc gtgctctgga cctgagcctc actcgcgcgt acgcgtttga
     3421 tgctcgagaa atcgagcagg gtctggttga acgtcagccg cgcgtcgtag gtgctgaacg
     3481 ggggcacgac ggtgggcagc tggatcggcc cgcccttcgg aaacgggaga cccagcgcat
     3541 cgagatcgta gtctcgattc acccaggagc ccccggcggt cagaccggga aggagtgcgg
     3601 aacgcgtttg gcgcacgcgc gcccgcgccg cgtcggtttc gagcccggag agttgcacgg
     3661 gagcggcctg tccggtcgcc attctcacgg cgtcgccgaa gctcagcttg agctgggtct
     3721 gggccgccac ggcccgggga gcgaagagaa caccgagaac cagcatccag cgattacgca
     3781 tgattccatt ccccaatagg cgcgggggcg agcccccgca gcagcatgtc gaggtgcgct
     3841 tccacgtacc ggtcgccgtc gaagggctcc cggatgcacc ggtagagtga ttgactgtgg
     3901 accacggcca tgagcatcgg ggcgatgacc aaccggaccg tgaccatgac gtcgacctga
     3961 cggaattccc cggagtcgac cccgcgctgg aggacctgag cgaagagcag atgcccgcgg
     4021 cgaatcactt ctttgtagta gaaccgcgtc agctccggaa agttcgtcgc ctccgcgacg
     4081 atcaatttca cgacgccggc cagcgaccct ttcccgacgg tggcccacca gccccgcacc
     4141 atgtggcgca gaagctccgc ggagctcccc ttccagtcac gcacttcttg ctcggcggcc
     4201 gccaggatgg gcaccacggt ttggcggatg acttcctgga agagcgcttc cttgttctcg
     4261 aagtagaggt agatggtgcc cttggtgacg cccgcctcct tggcgacgtc atcgagacgg
     4321 gcggccgcga acccatgttg cgcgaacact tgcaacgcgg cgctcacgat ctcttcgggg
     4381 cgggcttcct tgcggcgctg ccaacggggc ttcatggggc tcttctttgg aggaatcatt
     4441 aataactgaa cggtgagtaa ttaataccgg ccccggacct cagcaagggc gtctggcact
     4501 tcctcacaaa ccgccctaga atggccccgg tgaccagccc tgcgcaggcc tattcccgcc
     4561 ccatcagggc catcgcggtg gtggtggcga tcgccctggc gctcaggcaa tacctgtccc
     4621 tgtccgtctc tccccccgca tacggccggc tcctcgcggt gcggatcggc acgctggttt
     4681 tggccatcgg gatcgcctgg ctggccacgc ccggccgctc cgtccgcacc ctcagagtct
     4741 acgccatggt gctgggactt tccctggccc tcgccggatg cttgagcgga ttgatcctcc
     4801 ccacacggtt ttgggaggtc gccggcttgt tacccgccgt cgtgctggga gtctcgctct
     4861 tcatcccgtg gccctggccc tggcaagcca ccttcgccac gtccgcgttt ctcatcgaat
     4921 gcctcacgct ccggtggatt cccgacgccg ccctgcccac cccgctcgcg gacgagggcg
     4981 ccttggtgct gctgttcagc gcgggcatcg ccagcaccat cgggtctcgc atcgcggaca
     5041 acgagcggag gcgtgtcgcg gagtccgagg cccggtttcg agcgctgttc gagggcgccg
     5101 gcgacccgat cgccgctctc gaacccgatg gccgggtaca tgatgc
//
LOCUS       DASUBP010000255         9846 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1289816, whole genome shotgun sequence.
ACCESSION   DASUBP010000255 DASUBP010000000
VERSION     DASUBP010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 9846)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9846)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9846
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1289816"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..846
                     /locus_tag="VFU75_09575"
     CDS             <1..846
                     /locus_tag="VFU75_09575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HEU5467130.1"
                     /translation="DRTPKLFIGGKQVRPDQGYSRKVAGPSGEVVGEVGDGNRKDIRD
                     AVEAAHKASGWGRGSGHARAQVLYYVAENLSARAEEFARRIAAQTGVSSDAAAGEVTA
                     SISRLFTYAAWADKYDGAIHHVPIRGVTLAMPEPIGVIGIAAPAANPLLGFVSLVAPA
                     VATGNTVVAIPSPTHPLSATDFYGVLETSDVPDGVINIVTGDRDPLIKVLAEHLDVDA
                     VWYWGTAAGVKAVEEASAGNMKRTWASDQTAHALDWLDPAWGEGQEFLRHATQVKNIW
                     IPYGE"
     gene            843..1463
                     /gene="pduL"
                     /locus_tag="VFU75_09580"
     CDS             843..1463
                     /gene="pduL"
                     /locus_tag="VFU75_09580"
                     /EC_number="2.3.1.222"
                     /inference="COORDINATES: protein motif:HMM:NF011652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HEU5467131.1"
                     /translation="MTSPVGSTVPIGVSNRHLHLSPADVTRLLGDGKSLTVDRMITQP
                     GQYAAKERIDVVGPKGKLTGMRVVGPARSATQVELALTDARTLGVAAHLANSGKLTGS
                     AGGVTLVGPAGSVTLSSGVIVAARHLHCGPQDAARLGVSDGDWIDVVCGTGSRRVVFE
                     GLLVRAGPTHATEVHLDSDEAAAAGIATGAEARIVGWRPAGGAVTP"
     gene            1460..1891
                     /locus_tag="VFU75_09585"
     CDS             1460..1891
                     /locus_tag="VFU75_09585"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEU5467132.1"
                     /translation="MITAIEHRVNYSETDQMGLVYHANYVIWLDMARTEHLRGTGVTY
                     KEMEEQGVYLAVTDLQVRYRRAARYDDLVRVRCWVREVASRRVTFGYAVERAGGDSAG
                     IPELLATAQTSLMALDRGHSPTRIPEHVRELLAPSSDPVRL"
     gene            1911..3917
                     /locus_tag="VFU75_09590"
     CDS             1911..3917
                     /locus_tag="VFU75_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU5467133.1"
                     /translation="MAAALVLALPLNAQQSAPVDEAAVGELARILAAADSRTYDDAIF
                     RDGLQQADPSIRRQAALAAGRIGDTAAVPILVQALADSAQGVRSAAAFALGLLKDPRA
                     IQPLLDMVQRASPEQQDFGTFEAVTAIAKIGGTEGARALQLIIDAGRPGQPNQAVSRA
                     LLEAWRLGAEGPVVSLIRYAQDPDNLVRWNAMFSLSRSGDPRALPSLLTALNDQDATV
                     RAVALRGITAHALDVARQPHAPVIARVRSLLTDRDAGVRINALRALGTFHDSTLAGAI
                     APLASDGTLGVAVVADTTLGVIGGAAAVVALKAHLASANFAVRRQAAIGLAEADSAQG
                     VAFADTLSTNTDWHWRSVAAEADGAARDRARLERLLSDADGRVVAAALNAMMRFIVDA
                     DPAFAARARGLLANADPAVRSLAADELGRHPSTGDVDALVAAYQRASGDPFDDARLSA
                     VRALCAIASASPPGLIAVTQRFFQSVSRPTDYLAWRMAVVSFPGAMPAWQTAPPIATG
                     KTDADYRDAARRYLYPALHGTANPTVTIETDRGSITIELLPAEAPLTVAAFLALVDRR
                     YFDGDHWHRVVPNFVIQDGDPRDDGWGGPGFALRDEISPQRFQKGSVGLALSGPDTGG
                     SQYFITLGQEPRLDGVYPIFGRVTSDLSVLDNVTQGDRIRSVHR"
     gene            3914..6760
                     /locus_tag="VFU75_09595"
     CDS             3914..6760
                     /locus_tag="VFU75_09595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467134.1"
                     /translation="MRRRWLAALAVLLMPCGLHAQYGYFGENKVQFKEFDWRLMQGPH
                     VDLHYYSGEEELAKVALAYAEESYSALSRRFNHEVPHRVPLIVYASHTDFEQTNILPY
                     VPPEGLLGVTDFAKERVTLPFTGSYYDFKHTIRHELVHVFQISVETQTYHRYPREHHA
                     ALPLWWTEGLAEYFSIGTDNTGEMLLRELTVTGQLPTLDQLTYAEGGAIYPLGAKIHE
                     FLAEKYGEWRILEVYDQTWKYETFDKLMEAVYGQSLDQISEGFRYWMRQKYYPAVSTS
                     QPLALQARRIGTLAIKPTVVWDSADTAKSRVLYFSPADGYTDIYAARLDGEGKRAVVK
                     GERTPEFESFHFFDSRLDVYQNRLLAFTSKYLDRDALFIWDLLRQKKVGRFQFPGLVG
                     ILSPSWTPDGQSVVFSGLATDGYSDLYRLWIADGRLERLTNDRYQDVDPSVSPDGKRV
                     VFASDRTSFGIDGAKNLFTLDLANGAIRYLTYGDWKDTGPRWDWGTGRIWFSSDRDGS
                     SQIYAVDSTGTGRRVTEVLGGAFDPQYVPAESTVVFGGFSDLSFGIYRTRVAADTTGP
                     AVSLASNPQPPDWAWPELNAAVSVTGRPEPYRQRFTLDFAAGDAIIVPGLGAAQGADF
                     LFSDLLEDHLALLELSSFQGNGLGNFFGNINGTLMYLNQAHRINWGVGVFRLRGQFFE
                     GDLSTVYDETSTGVLGQLRYPLDRFRRLEVEYRLEHSDRFDFGSIDVLNGGDPTTLHR
                     IGWLSSNYLSFVKDNTLWLDTGPIDGERYAVTGGVVNDVSHGRFDSWVAFGDYRRYVR
                     TSLRSGFAFRAMAYYGGGERPRRTNIGGSWGLRGYPYYGYVAGTRAWMANLEWRFPIT
                     DYLDLGFPFGPLRFPGVQGAFFGDLGRAWTPLTQDRGTLGSSGLGFRMPVGPGFVLRL
                     DVGYRFSYGDVSEYSLPFNFQGRHFVDFFFGYNY"
     gene            6817..7479
                     /locus_tag="VFU75_09600"
     CDS             6817..7479
                     /locus_tag="VFU75_09600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467135.1"
                     /translation="MALICAMSACRTSSLVPVPLAPASPDSANMWALGTAPKHAAGIR
                     FKWRYEDRKLNAAGRATARLAPPDSARLDYAATLGLSSGAGVVIGDSVIWADPDQDFR
                     SFIRGVPVFWATMGVARPAPAGAVVSTGVVGGTDRPRRAWRYATGADTLTYVAGNAAL
                     DVEWRSGSTVVARSHTDLDGRGWPARAQVEFPEAGARFELTVVGIDTTVVVSPALWRS
                     RR"
     gene            7409..7945
                     /locus_tag="VFU75_09605"
     CDS             7409..7945
                     /locus_tag="VFU75_09605"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HEU5467136.1"
                     /translation="MSSQWWGSTRRSWYRRLSGAVAGSVTLASLTGCLYGFAGGGLPN
                     IKTVAIQPFDNQTPVPQLTTEVNDAIRQAFESRLGLRQAGEQTADALVRGRIVSYQAD
                     VPLAFVAGQGQAPVTRRQVQVTVDVEVLDQKEGKTIWQRQGLTVQGDYQPPAEADGRK
                     QALDKLVTEIVEGAQSQW"
     gene            7939..8322
                     /locus_tag="VFU75_09610"
     CDS             7939..8322
                     /locus_tag="VFU75_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467137.1"
                     /translation="MVTGRPQRSHSRRGASSLGCLVSLLIFVAALYYGVHIGEVYFRY
                     YRLVDEMDSQARFASAIDNGTIRRRLEAAIEDIGLPSAAADGLVITRTGDPRKIHIET
                     HYTERVQLPLFDHTFTLNPSADESL"
     gene            8336..8812
                     /gene="rfaE2"
                     /locus_tag="VFU75_09615"
     CDS             8336..8812
                     /gene="rfaE2"
                     /locus_tag="VFU75_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HEU5467138.1"
                     /translation="MHTEGKVRSWDGAARWRLGEGGRVVFTNGVFDLLHVGHVALLEA
                     ARREGASLIVGVNSDASVRRLNKGPDRPLVPQDARARVLAALAAVDCVVIFDQDTPLE
                     LIKHLRPDVLVKGADYERAAIVGADEVEGWGGRVVRVPLVPGHSTTELARRRGAGS"
     gene            8821..9435
                     /locus_tag="VFU75_09620"
     CDS             8821..9435
                     /locus_tag="VFU75_09620"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HEU5467139.1"
                     /translation="MGTRSAGKAREIRSLLAGLPFEVQFPADWFLGRLPEEEDLESGT
                     TYAENAVLKARYFAERVRGQIATVADDSGLEVDALDGAPGVHSARWAGAGATDAAQDA
                     RNNAKLVAALQGVPEKRRTARYRCVVAFLDRLNGTPELVEATCEGRIILEPRGAGGFG
                     YDPYFFCPELGLTFGEASLPAKERVSHRGRAFRALAEMLLRRGV"
     gene            9495..9568
                     /locus_tag="VFU75_09625"
     tRNA            9495..9568
                     /locus_tag="VFU75_09625"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9529..9531,aa:Pro,seq:tgg)
     gene            9608..>9846
                     /locus_tag="VFU75_09630"
     CDS             9608..>9846
                     /locus_tag="VFU75_09630"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEU5467140.1"
                     /translation="MTSAVEGLHGQSVGISLTVKDIEKSLQWYVSLGFKEERKLERDG
                     QLRGYAVGAGDVHILLNKDDGAKGWTRVKGEGFSMS"
BASE COUNT         1499 a         3184 c         3467 g         1696 t
ORIGIN      
        1 gaccggactc ccaagctctt catcggcggc aagcaggtcc gccccgacca gggctacagc
       61 cgcaaggtgg ccggcccgtc aggtgaggtg gtgggcgaag tcggcgacgg caaccgcaag
      121 gacatccgcg acgccgtgga agcggcgcac aaggcatcgg ggtggggaag gggctccggg
      181 cacgcacggg cgcaagtcct ttactacgtc gccgagaatc tctccgcccg ggcggaggag
      241 ttcgcccgcc ggattgcggc ccaaaccggc gtatcgtccg atgccgcggc gggagaagtc
      301 accgcctcca tctcccggct cttcacctac gcggcctggg ccgacaaata cgacggcgcg
      361 attcatcacg tcccgattcg gggcgtcacg ctcgcgatgc cggagccgat cggggtgatc
      421 ggcatcgcgg cgccggcggc caacccgctg ctcgggttcg tctctctcgt ggcacccgcg
      481 gtcgccaccg gcaacaccgt ggtcgccatt ccgtcgccga cacatcccct gtccgccacc
      541 gacttctatg gcgtgctcga gacgtccgac gtgccggatg gcgtgatcaa catcgtgacg
      601 ggagaccgcg acccgctcat caaggtgctc gcggagcatc tggacgtcga cgccgtgtgg
      661 tattggggaa cggcggcggg tgtgaaggcc gtcgaagagg cgtcagccgg caacatgaaa
      721 cggacctggg cctccgacca gacggcccac gcgctggact ggctcgatcc cgcctggggt
      781 gaggggcagg agttcctgcg gcatgccacc caggtgaaga acatctggat cccctacggg
      841 gaatgacgtc tcccgtcggc tccaccgtcc ccatcggggt ctcgaaccgc catctgcacc
      901 tctctcccgc cgacgtcacc aggcttctcg gtgacgggaa gtcgctcacc gtcgatcgga
      961 tgatcacgca gccgggacag tatgccgcca aggaacgaat cgacgtcgtg ggccccaaag
     1021 ggaagctcac cggcatgcgt gtggtcgggc cggcgcggag cgccacccag gtggagctcg
     1081 ccctcaccga cgcccgcacg ctcggcgtcg ccgcgcacct cgccaactcg gggaagctca
     1141 ccggctcggc ggggggcgtg actctggtcg gccccgcggg ctccgtcacc ctctcgagtg
     1201 gagtcatcgt cgccgcccgc cacctccatt gcggtcctca agacgccgca cgtctggggg
     1261 tgtcggatgg ggactggatc gacgttgtgt gcggtacggg cagccggcgg gtggtattcg
     1321 agggcctcct cgtgcgcgcc ggcccgacgc acgccaccga ggtccatctc gactcggatg
     1381 aagcggctgc cgccggcatc gccacgggcg ccgaagcccg gatcgtggga tggcggcctg
     1441 cagggggggc ggtcaccccg tgatcaccgc catcgagcac cgggtgaact actcggaaac
     1501 ggaccagatg gggttggtct atcatgccaa ttacgtcatc tggctggata tggcgcggac
     1561 ggaacacctg cgcgggactg gggtgacgta caaggagatg gaagagcagg gggtgtacct
     1621 ggcggtgacg gacctccagg tgcgctaccg gcgcgcggcg cgatacgacg acctggtgcg
     1681 cgtccgctgt tgggtccggg aggtggcgtc acgtcgggtc accttcggat atgcggtcga
     1741 gcgggccggc ggggactccg ccgggattcc cgagctcctc gccaccgcgc aaacgtcgct
     1801 catggccctc gatcgagggc actcgcccac ccgcattccg gagcacgtgc gtgagttgct
     1861 ggcgccgtcg tcggaccccg ttcgcctgta gccggcgtgc gccgtggctg ttggcggcgg
     1921 ccctcgtcct cgcgctcccg ctcaacgccc agcaaagcgc ccccgtcgac gaggcggctg
     1981 tgggcgagct cgcccggatc ctcgcggcag ccgacagccg gacgtacgat gatgcgatct
     2041 tccgcgacgg cctccagcag gccgacccgt cgattcgccg ccaggccgcg ctcgcggcgg
     2101 gccgcatcgg ggatacggcg gcggtgccca tcctggtgca ggccctcgcg gactccgctc
     2161 agggtgtccg ttcggcggct gccttcgcgc tcggactcct caaggacccg cgggccattc
     2221 agccgttgct cgacatggtg cagcgcgctt cacccgagca gcaggatttc ggcaccttcg
     2281 aggccgtcac ggcgatcgcg aaaatcggcg gcaccgaggg cgcgcgggcc ctgcagctga
     2341 tcatcgacgc gggacgcccg gggcagccga accaggctgt atctcgcgcg ttgctcgagg
     2401 cctggcgcct cggagccgag ggaccggtgg tctcgctcat ccggtatgcg caggaccccg
     2461 acaatctggt gcgctggaac gcgatgttct ccctgtcgcg cagcggtgac ccgcgggcct
     2521 tgcccagtct gctgacggct ctgaacgatc aggatgcgac ggtgcgcgcc gtcgctctgc
     2581 gcggcatcac ggcgcacgcg ctggacgtcg cgcgccaacc gcacgcgccg gtgatcgcgc
     2641 gggtgcgttc gctcctgacc gaccgcgacg ccggcgtccg gatcaacgcg ctgcgcgcac
     2701 tcgggacctt ccacgattcg accctggccg gcgcgatcgc gcccctcgcg tccgacggca
     2761 cgctgggcgt ggcggtggtc gccgatacga cgctcggtgt gatcggggga gcggcggcgg
     2821 tggtggccct gaaggcccat ctcgcctccg cgaatttcgc cgttcgccgc caggcggcca
     2881 tcggactggc cgaagccgac tcggcgcagg gggtggcgtt cgccgacacg ctgtcgacca
     2941 ataccgattg gcattggagg agcgtcgccg ccgaggccga tggggcggcg cgcgaccgcg
     3001 cccgtctcga gcggctcttg tccgacgccg acggtcgcgt cgtcgccgcc gcgctcaacg
     3061 cgatgatgcg attcatcgtc gatgccgatc ctgccttcgc ggcgcgggcg cgcggactcc
     3121 tggcgaacgc cgatcccgcc gtgcggagtc tcgccgccga tgagctcggg cggcatcctt
     3181 cgacgggtga cgtggacgca ctcgtcgcgg cgtaccagcg ggcgtcgggt gatccgttcg
     3241 acgatgcccg cctgtctgcg gtccgggcgc tctgtgccat tgcgtccgcc agtccgcccg
     3301 gcctcatcgc cgtgacccag cgtttcttcc agtcggtgtc ccgtcccacg gactacctcg
     3361 cgtggcggat ggcggtggtc agcttcccgg gggcgatgcc cgcctggcag acggcacccc
     3421 cgatcgccac cgggaagacg gacgcggatt accgcgatgc cgcccggcgc tacctgtatc
     3481 ccgcactgca cggcaccgcg aacccgaccg tcaccatcga gaccgaccgc gggtccatca
     3541 ccatcgaatt gctgccggcc gaggccccgt tgacggtcgc ggcattcctc gccctggtcg
     3601 accgccggta cttcgacggc gatcattggc accgggtggt gccgaacttc gtgattcagg
     3661 acggggatcc gcgcgatgac gggtggggtg gaccggggtt cgcacttcga gatgagatct
     3721 cgccccagcg ctttcagaag ggttcggtgg gactggccct gtccggtcct gacaccggcg
     3781 gcagccagta cttcatcacg ctggggcagg agccgcggtt ggacggagtg tatccgatct
     3841 tcgggcgggt gacctcggac ctctcggtgc tcgacaacgt gacgcagggc gaccgcattc
     3901 gcagcgtgca ccggtgagac gccggtggct cgcggcgctc gccgtactgc tgatgccgtg
     3961 cgggttgcac gcccagtacg ggtacttcgg tgagaacaag gtccagttca aggagttcga
     4021 ctggcggttg atgcagggcc cgcacgtgga cctgcactac tactcgggcg aagaagagct
     4081 ggcaaaggtc gccctggcgt acgccgaaga gagctactcg gccctgtcgc ggcgcttcaa
     4141 tcacgaggtc ccgcaccggg tgccgctcat cgtctacgcg tcgcacaccg atttcgagca
     4201 gacgaacatt ctgccgtacg tgccgcccga aggcctgctc ggcgtcaccg acttcgccaa
     4261 ggagcgcgtg acgctcccgt tcaccggcag ctactacgac ttcaagcaca ctatccgtca
     4321 cgagctggtg cacgtctttc agatcagtgt ggaaacccag acctatcacc ggtatccccg
     4381 ggaacaccat gcggcgcttc cgttgtggtg gaccgagggg ctggcggaat acttctcgat
     4441 cgggaccgac aacaccgggg agatgctcct gcgcgagctc actgtcaccg ggcagctccc
     4501 gacgctggat cagctgacgt acgccgaggg gggagcgatt tatccgctcg gcgccaagat
     4561 ccacgagttt ctcgcggaga agtacggcga gtggcggatt cttgaggtgt atgaccagac
     4621 gtggaagtac gagaccttcg acaagctgat ggaggcggtc tacgggcagt ccctggacca
     4681 gatctccgag gggttccggt actggatgcg gcagaagtac tacccggcgg tgagtacgtc
     4741 tcagccgctg gcgctgcagg cccggaggat cgggacgctg gccatcaaac ccacggtggt
     4801 ctgggactcc gcggacaccg cgaagagccg ggttctctac ttctcgccgg ccgacgggta
     4861 cacggacatc tacgcggcgc ggctcgacgg tgaagggaag cgggcggtgg tgaagggcga
     4921 gcgcacgccc gagttcgagt ccttccattt cttcgattcc cgcctcgacg tctatcagaa
     4981 ccgcttgctc gccttcacca gcaagtatct ggaccgcgac gcgctcttca tctgggacct
     5041 gctccggcag aagaaagtcg ggcgattcca attcccgggg ctcgtgggga ttctgtcgcc
     5101 gtcctggacc cccgacgggc agagcgtcgt gttctcgggc ctggccaccg acgggtactc
     5161 ggacctgtac cgcctctgga tcgccgacgg acggctggag cgcctgacca acgatcggta
     5221 tcaggatgtg gacccgtcgg tgagccccga tgggaagcgg gtagtcttcg cgtcggaccg
     5281 cacctcgttc gggatcgatg gcgcgaagaa tctcttcacg ctcgacttgg cgaatggggc
     5341 gatccggtat ctcacctatg gggactggaa agacaccggc ccgcggtggg attgggggac
     5401 cggacgcatc tggttctcga gtgatcgcga cgggtcctca cagatctacg ccgtcgactc
     5461 gaccggcacg ggacgccggg tgacggaagt cctcgggggt gccttcgacc cgcagtacgt
     5521 tccggcggaa tcgaccgtcg tcttcggcgg attctccgat ctgtccttcg ggatctaccg
     5581 gacgcgcgtg gccgccgaca ccactggccc ggccgtgagc ctggcgtcaa atccgcagcc
     5641 gcccgactgg gcgtggcccg agctcaacgc cgccgtgtcg gtgacgggga ggccggagcc
     5701 gtaccgccag cgcttcacgc tggatttcgc cgcgggggac gcgatcatcg taccggggtt
     5761 gggggccgcg caaggcgccg acttcttgtt ctcggatctg ctcgaagacc atctcgcgtt
     5821 gctcgagctg tcgtccttcc agggcaacgg gctcggcaat ttcttcggca acatcaatgg
     5881 cacgctgatg tacctgaacc aggcgcaccg gatcaactgg ggcgtcggtg tgttccgcct
     5941 gcgcgggcag ttcttcgagg gcgacctgtc gacggtctat gatgagacgt cgaccggagt
     6001 gctggggcag ctgcggtatc ccctcgatcg tttccgccgc ctcgaggtgg aataccggct
     6061 cgagcattcg gaccgcttcg atttcggatc gatcgacgtt ctcaacgggg gcgatccgac
     6121 cacgctgcac cggatcgggt ggctgtcatc caactacctg agcttcgtga aggacaacac
     6181 gctctggctc gacaccgggc cgatcgacgg cgagcggtat gctgtgacgg ggggcgtggt
     6241 caacgacgtc agccatggcc gattcgattc gtgggtggcg ttcggcgatt accgccggta
     6301 cgtgaggacg tccctgcgct ccgggttcgc gttccgggcc atggcctact acggtggcgg
     6361 cgagcggccc cggcgcacca acatcggcgg ttcgtggggg ctccgggggt acccgtatta
     6421 cggctacgtg gcggggaccc gggcctggat ggcgaatctg gagtggcggt tcccgatcac
     6481 cgattatctg gatcttggct tcccctttgg gccccttcgg tttccagggg tccagggagc
     6541 cttctttggc gacttgggtc gggcctggac gccgttgacc caggaccgag gcaccctggg
     6601 gtcgtccggc ttgggcttcc ggatgccggt agggcccgga ttcgttctcc ggctggacgt
     6661 cgggtaccgg ttctcctatg gagacgtttc agaatactcc ctgccgttta actttcaggg
     6721 gcgacacttc gtcgacttct ttttcggata taactactga gcgggcaacg atttctgctt
     6781 gaccgtgccg gggcgccttc ctacattggc ggctgggtgg cactcatctg tgccatgtcg
     6841 gcatgccgga cgtcgtcgct cgtaccggtg cctctcgcgc ccgcttctcc cgattcagct
     6901 aacatgtggg cgctggggac cgcgccgaag cacgccgccg ggatccggtt caagtggcgg
     6961 tacgaggatc ggaagctgaa cgcggccgga cgagcgaccg cgcgtctcgc ccccccagac
     7021 tcggcgcgcc tcgattacgc ggcgacgttg ggtctttcgt cgggcgcggg tgtggtgatc
     7081 ggggattcgg tgatctgggc cgatccggac caggacttcc ggtcgttcat tcggggcgtg
     7141 ccggtgtttt gggcgacgat gggtgtcgcg cggccggcgc cggcgggtgc ggtggtaagt
     7201 accggcgtcg tcggcggtac cgaccggccg cggcgggcgt ggcggtacgc gactggtgcg
     7261 gacactctca cgtacgtggc agggaacgcg gcgctcgacg tcgaatggcg cagcgggtcg
     7321 accgtggtgg cgcggagcca cacggatctt gatgggcgcg gctggcccgc ccgcgcgcag
     7381 gtggagtttc ccgaggcggg agcgcggttt gagctcacag tggtggggat cgacacgacg
     7441 gtcgtggtat cgccggctct ctggcgcagt cgccggtagc gtcacgctcg cgagcctgac
     7501 cgggtgcctg tacggcttcg ccggtggtgg gctccccaac atcaagaccg tcgcgattca
     7561 gcctttcgac aatcagacgc cggtcccgca gctgacgacc gaggtcaatg acgcgatcag
     7621 gcaggcgttc gagagccggc tgggattgcg tcaggcgggt gagcagacgg cggacgcgct
     7681 ggtgcgcggg cggatcgtct cgtatcaggc ggatgtgccg ctggcgttcg tggcgggaca
     7741 aggccaggcg ccggtcaccc ggcggcaggt acaggtgacg gtggacgtcg aggtcctcga
     7801 tcagaaagag ggcaagacga tctggcagcg gcaggggttg accgtccagg gcgactacca
     7861 gccgcccgcg gaagcggacg gacggaagca ggcgctcgac aagctggtga cggaaatcgt
     7921 ggagggagcc caatcgcaat ggtgaccgga cggccccaac ggtcgcactc ccggcggggt
     7981 gcttcgtccc tcggctgcct ggtgtcgctc ctgatcttcg tcgcggccct ctactatggg
     8041 gtccacatcg gcgaggtcta cttccggtat taccgtctgg tcgacgagat ggacagccag
     8101 gcccggtttg catcggccat cgacaacggc accattcggc ggagactcga agccgccatc
     8161 gaggacatcg gacttccctc cgcggcggct gacgggctgg tgattacccg cacgggtgat
     8221 cctcgaaaga tccacatcga gacccactac accgagcggg tacagttgcc gctcttcgat
     8281 cacacgttca cgctcaatcc atcggctgac gagtccctct agacccgggt ccccattgca
     8341 tacggaaggg aaggtccgtt cctgggacgg cgcggcgcgc tggcgcctgg gggaaggcgg
     8401 gcgcgtcgtg ttcacgaacg gcgtgttcga tctgctgcac gtggggcacg tcgcgctgct
     8461 ggaggcggca cggagagaag gcgcgtcgct gatcgtcggc gtgaacagcg acgcctcggt
     8521 ccggcgtttg aacaagggcc ccgaccggcc gctcgtcccg caggatgcgc gggcgcgcgt
     8581 gctcgccgcg ctcgcggcgg tggactgcgt ggtcatcttc gaccaggaca cccccctcga
     8641 actgatcaag cacctgcgtc ccgacgtgct ggtgaagggc gccgactacg agcgcgccgc
     8701 catcgtcggg gcggacgagg tggaggggtg gggcggcagg gtggtgcggg tacccctcgt
     8761 tcccgggcat tccacgaccg agctggcgcg gcgacgcgga gccggatcct gaagttcctg
     8821 gtggggacgc gaagtgccgg gaaggcacgg gagatccgct ccctcctcgc gggattgcct
     8881 ttcgaagtcc aattcccggc cgactggttt ctgggccgcc tgcccgagga agaggacctg
     8941 gaaagcggga cgacgtacgc cgagaatgcc gtgctgaagg cacggtactt cgcggagcgg
     9001 gtgcgtgggc agatcgcgac cgtggccgac gattcagggc tcgaggtcga tgccctggac
     9061 ggggctcccg gggtccattc ggcgcgctgg gccggtgccg gcgcgaccga tgctgcccag
     9121 gatgcccgga acaatgcgaa gctggtggcg gccctccagg gggtgcccga aaagcgccgg
     9181 acggcgcgat atcgctgcgt ggtagccttc ctcgaccgac tgaacgggac cccggagctc
     9241 gtggaggcca cctgtgaagg ccggatcatt ctggagcccc ggggggcggg gggattcggg
     9301 tacgacccgt acttcttctg ccccgagctg ggcctgacat ttggagaggc ttcgttgccg
     9361 gcgaaggagc gggtttccca tcgcggccgg gcgtttcgtg ccttggcgga aatgttactt
     9421 cgccgaggtg tttagtttta tagtgtgcag ttcctaagcg actgggcgcc tgtgcgcctt
     9481 ggcgcctagt agttcggggc gtggcgcagt tcggtagcgc gcctggtttg ggaccaggag
     9541 gccgcgggtt cgaatcccgc cgccccgact cacctcatcg ccacatcatt ccactctcag
     9601 gagctttatg acttcagcgg ttgaaggcct gcatgggcag tcggtcggga tctcccttac
     9661 cgtcaaggat atcgagaaga gcttgcagtg gtacgtgagt ctcgggttca aggaggagcg
     9721 gaagctcgag cgcgacggcc agctgcgtgg ttacgcggtc ggcgcggggg acgtgcacat
     9781 cctcctcaac aaggatgacg gcgccaaagg gtggacccgc gtgaagggtg aggggttctc
     9841 gatgtc
//
LOCUS       DASUBP010000256         4164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_581534, whole genome shotgun sequence.
ACCESSION   DASUBP010000256 DASUBP010000000
VERSION     DASUBP010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4164
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_581534"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1400)
                     /locus_tag="VFU75_09635"
     CDS             complement(<1..1400)
                     /locus_tag="VFU75_09635"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU5467141.1"
                     /translation="MGVSVKQDTEFRREITRLDATALVVGTMIGSGIFLVSADIMRQV
                     SSPGVMLLVWALSGVITLMGALSYGELAAMFPNAGGQYVYLREGISPLFGYLYGWTLF
                     AVIQTGTIAAVAVAFAKFTAIFWPGLSPDVWFGAAVNLGSGPISIGVSPQRLLAIASV
                     IALTWINARGVRSGARIQTTLTIVKTLSLAALVVLGLLIGRNATAIAANFGANFWPAK
                     GLTLALLPIIGSAMVGSLFSMDSWNNVGFAGSELKNPKKDLPLSMAMGTLTVTILYLL
                     ANVAYLVTLPGDSIAHAPQDRVGTAALQAMFGDPGLYLMAGAIMISTFGCNNGLILSG
                     ARVFYAMAKDSLFFQKAGTLDPKYQTPKFALWIQALWTSVLCLSGTYNELLTYVIAAQ
                     LLFYACTALGLFMMRKRRPDAERPVRAPGYPWIPGLYLVSTLVLCIDLLFTQTKYAGI
                     GLIIVALGVPVYFVTKG"
     gene            1499..2113
                     /locus_tag="VFU75_09640"
     CDS             1499..2113
                     /locus_tag="VFU75_09640"
                     /inference="COORDINATES: protein motif:HMM:NF013982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HEU5467142.1"
                     /translation="MRLLPREEEFFDLFAGVAQRNKEAAHLLRQLFDAAPDRRTASVE
                     AIKRLEHEADQITHEVVNRLDRTFITPLDREDIHQLASDLDDVMDVMDGVARRSQIFR
                     VGATPDGVKQLAEVIERMVGVLAEGVACLKKGDDVMKYCVEAKKLEEEGDAIYHQSLG
                     KMFETEKNAIELIKWKEIYDNLERTLDEAEDVANVLESITLKHG"
     gene            2128..3153
                     /locus_tag="VFU75_09645"
     CDS             2128..3153
                     /locus_tag="VFU75_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HEU5467143.1"
                     /translation="MTVTFVLAIIVVALVFDFFNGFHDAANSIATVVSTRVLSPAAAV
                     VWAAAFNFIAVFMFGTAVAKTMGTGLVDIAIVDGNVILSGLIGAILWDLITWWLGLPT
                     SSSHALIGGYGGAAVAKAGFTALVFPGKWLSTLLFIVVAPLMGLILALGLTVALSWVL
                     RRARPRKVDGTFRVLQFFSAAAYSLGHGTNDAQKTMGIIVGLLVAEQARFTTFPVQAL
                     HLTNLNVIPFWVVVACGAAMGLGTVSGGWRIVKTMGQRLTRLKPFGGFCAETAGAITL
                     FMSSHFGIPVSTTHTITGAISGVGAAQRLSAVRWGVAGQIVWAWILTIPAAFFIAFVC
                     YLVVHAI"
     gene            complement(3189..3866)
                     /locus_tag="VFU75_09650"
     CDS             complement(3189..3866)
                     /locus_tag="VFU75_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467144.1"
                     /translation="MFDSLPPGWVVDQLNEMDPIWQPLGARGMNYYSFGAPVPPDPYA
                     TRSDPLSPLYQAWLGAYVVVGALDSASADSAALQQHWVVALTERDQQAWLAGMGDPSP
                     VAQAVLPLRRSEIRIAGAMRPLYVGEMRTHSDLSQGGTPLSMSLGMPPAFQWQGSLDA
                     FHDVVLHVQGAIWYDPARRVTIIVYAASSSYHTRDGKLHDNGPALDSLLRAAMGRARI
                     VAASGNQ"
BASE COUNT          764 a         1367 c         1332 g          701 t
ORIGIN      
        1 cccttggtca cgaaatacac cgggactccc agcgccacga tgatcagccc gatccccgcg
       61 tacttggtct gcgtgaacag cagatcgatg caaagcacga gcgtcgagac gagatacaac
      121 cccggaatcc acggataccc cggcgcgcgc accggccgct cggcgtcagg ccgtctcttc
      181 cgcatcatga acaagccgag cgccgtgcag gcgtagaaga gcagctgcgc ggcgatgacg
      241 taggtcaaca gctcgttgta ggtgcccgag agacagagca ccgaggtcca cagcgcctgg
      301 atccagaggg cgaacttcgg cgtctgatac ttaggatcca gcgtgccggc cttctggaag
      361 aacaggctgt ccttggccat cgcatagaac acgcgggcac ccgagagaat cagcccgttg
      421 ttgcacccga aggtcgagat catgatggcg cccgccatca agtagagtcc cgggtctccg
      481 aacatggcct gcaaagccgc ggtcccgacg cgatcctgcg gcgcatgcgc gatcgaatcg
      541 ccgggcaacg tgacgagata ggcgacgttc gcgagcaggt agagaatcgt gaccgtcagc
      601 gtccccatcg ccatggacag gggcaagtcc ttcttcggat tcttcagctc cgagcccgcg
      661 aatcccacgt tgttccacga atccatggaa aagagcgatc ccaccatcgc ggatccgatg
      721 atcggcaaca gcgcgagcgt caggcctttc gcgggccaga aattggcgcc gaagttcgcc
      781 gcaatcgccg tggcgttccg gccgatcaac agccccagca ccaccagtgc cgccagcgaa
      841 agtgtcttga cgatcgtgag cgtcgtctgg atgcgcgcgc cggagcgaac cccgcgggcg
      901 ttgatccagg tcagcgcgat caccgaggcg atggcgagca ggcgctgcgg ggacaccccg
      961 atcgaaatcg gccccgagcc cagattgact gcggcgccga accagacgtc gggggaaagg
     1021 ccgggccaga agatggccgt gaacttcgcg aaggcgacgg ccaccgccgc gatggtgccc
     1081 gtctggatca ccgcgaagag ggtccagcca tacagatagc cgaagagggg cgagatgccc
     1141 tcacgcaggt agacgtactg gccgcccgcg ttcgggaaca tcgcggcgag ctcaccgtac
     1201 gacagcgcgc ccatcagcgt gatcacgccc gacagggccc agaccaggag catgacgccc
     1261 ggactggaca cctgccgcat gatgtccgcg gaaaccagga aaatccccga gccgatcatg
     1321 gtgcccacga cgagcgccgt ggcgtcgagg cgggtgatct cccggcggaa ctccgtgtcc
     1381 tgcttcacac tgacacccat cgggtctccc ctttcggtcg ggccctaacc tagtggctcc
     1441 tcccgggggc cgctatgttt tccaccgtcg gtcatcccct tcagccaatg aggtcgtcgt
     1501 gcgcctgctc cctcgggaag aggaattctt cgacctattc gcgggtgtcg cccagcgcaa
     1561 caaggaggcg gcccacctcc tccggcagct gttcgacgca gccccggacc ggcggaccgc
     1621 atcggtcgag gccatcaaga gactcgagca cgaggccgac cagatcaccc acgaagtggt
     1681 gaaccgcctc gaccgcacct tcatcacgcc cctggaccgc gaagacatcc atcaactggc
     1741 ttccgacctg gacgacgtga tggacgtgat ggatggagtc gcgcgccgct cccagatctt
     1801 ccgcgtcgga gcgacacccg acggcgtgaa acagctcgcc gaagtcatcg agcgcatggt
     1861 gggcgtcctg gcggaaggag tggcgtgtct caagaaaggt gacgacgtga tgaagtactg
     1921 cgtcgaagcc aagaagctcg aggaagaagg ggacgccatc taccaccaat cgctgggcaa
     1981 gatgttcgaa accgagaaga acgcgatcga gctgatcaag tggaaagaga tctacgacaa
     2041 tctggagcgg accctggatg aggcggagga cgtcgccaat gtcctcgagt ccatcaccct
     2101 gaagcacggc tgacgcatcg gatgtccatg accgtcacgt tcgtcctcgc gatcatcgtg
     2161 gttgccctgg tcttcgactt cttcaacggc tttcacgacg ccgcgaactc catcgcgacg
     2221 gtggtttcga cgcgggttct ttcccccgcg gccgcggtgg tctgggccgc cgcattcaac
     2281 ttcatcgccg tgttcatgtt cggcaccgca gtggcgaaga ccatggggac gggcttggtc
     2341 gacatcgcca tcgtcgacgg caacgtcatc ctctccgggc tcatcggcgc catcctgtgg
     2401 gacctcatca cctggtggct gggacttccc acatcgtcct cccacgccct gatcggcggg
     2461 tacggcggcg ccgcggtggc caaggcggga ttcacggcgc tcgtcttccc cggaaagtgg
     2521 ctctcgacgc ttttgttcat cgtcgtggcg cccctgatgg gactcattct cgccctcggg
     2581 ctcaccgtcg cgctctcgtg ggtcctccgc cgggctcgcc cccgcaaagt ggacggcacc
     2641 ttccgcgtcc tccaattctt ctcggcggcg gcctactctc tgggccatgg caccaacgac
     2701 gcccagaaaa cgatgggcat catcgtcggc ctgctggtgg ccgagcaggc gcgctttacg
     2761 acgttcccgg tgcaagccct tcacctcacc aacttgaacg tgatcccctt ctgggtggtg
     2821 gtggcgtgcg gggcggcgat ggggctgggg acggtgtcag ggggttggcg gatcgtgaag
     2881 acgatgggtc agcgactgac ccggctgaag ccgttcggcg gattctgcgc cgagaccgcg
     2941 ggggccatca cgttgtttat gtcatcgcac ttcgggattc cggtgagcac gacgcacacc
     3001 attaccggcg ccatctcggg agtgggggcg gctcagcggc tgtcggcggt gcggtggggt
     3061 gtggcgggcc agatcgtgtg ggcgtggatt ctcaccatcc cggcggcgtt cttcatcgct
     3121 ttcgtctgct acctcgtcgt tcacgccatc tagactcctt cgcccgcgtc gccgcgggtt
     3181 cccccgagct attggttccc gctcgccgcc acgattcgcg cacgtcccat tgcggcccgc
     3241 aggagggagt cgagcgcggg tccattgtca tgcagtttcc cgtcccgcgt gtgataggag
     3301 gacgaggccg cgtacacgat gatcgttacg cgccgggccg ggtcatacca gatcgccccc
     3361 tgcacgtgga gcaccacgtc gtggaaggcg tccaggcttc cctgccactg aaatgccggt
     3421 ggcattccca ggctcatgga caagggcgtt ccaccctggc tgaggtcgct gtgggttcgc
     3481 atctcaccca cgtacagcgg ccgcatggcc ccggcgatgc ggatttccga gcggcgcaga
     3541 ggaagcacag cctgcgcgac cggtgaagga tctcccatcc cggcgagcca cgcctgctga
     3601 tcccgctccg tgagtgccac cacccagtgt tgctgaagcg cggcgctatc ggcggacgcc
     3661 gaatcgagag cgcccaccac gacgtaggcg ccgagccagg cctggtagag cggggagagc
     3721 ggatcggacc gcgtggcgta cgggtcaggt gggaccgggg caccgaacga gtagtagttc
     3781 atcccgcgcg cacccagcgg ttgccagatg gggtccatct cgttcagctg gtcgacaacc
     3841 catccgggtg ggagggagtc gaacacgacg cgagggagtc cggcagtgcc ctgggtctga
     3901 gaatgtgccg gtgccgccgt aagcagcacg agcgccgcaa ttgggaaggc tcgtcgagcg
     3961 aatagtgaca tcgggtgagg gtagcgggcc cccgtcaccc caccgtcacg accggatcag
     4021 ccgaccttca gcgtgagcat ccggagctcg gtcatctcct gaatggccca gtgaaccccc
     4081 tcgcgaccca gtcctgaatc cttcgcccca ccatacggca accggtcaat ccggaagccg
     4141 gggatgtcgt tgccgttcac ggcg
//
LOCUS       DASUBP010000257        13546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1058657, whole genome shotgun sequence.
ACCESSION   DASUBP010000257 DASUBP010000000
VERSION     DASUBP010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 13546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13546
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1058657"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1786
                     /locus_tag="VFU75_09655"
     CDS             <1..1786
                     /locus_tag="VFU75_09655"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5467145.1"
                     /translation="RGPQVIFITMLEATDVLDARRRRPVTVADLVVLCLIGAAIGGVV
                     AFAREFGAPYVQAIHIDLSPAALPKYTLYSLSRGVFALIISYGFALAFGWAAASSPTA
                     ERFLLPLLDILQSIPVLGFLPGLVLGLVALFPSRNAGLELAAILMIFTAQAWNLALSF
                     YNSLKSVPEPLREASRMAGWSPWQIFRKLEVPSAAPGLVWNGMLSMAGGWFFLMVNEA
                     FQLGNRDYRLPGIGSYMSVAVEQGDRRAIILALIAMILMIVAVDRLLWRPVVVWAQRF
                     RLDDSSGETTQTSWLLEFLRRSRLRMQAHNVRKSAARTARRATRPLRRSLRTAERILR
                     RVPRPKPRRALLIRVSGALLLALVLAGGVWGVWRLAGLLLQLSGPAWVNLAEAAGLTF
                     VRVLVAVALGTAWALPAGILIGRSIRIAEVLQPVIQIVASFPAPMIFPLVVVGLALLG
                     VQLNLGSIVLMILSSQWYILFNVISAVAALPPQLSEAAQVFKVSGWRRWRALYLPAAF
                     PALVTGWITAAGGAWNASIVSEYITAGGSLRSTLGLGSLISDATARADFPLLAGGITL
                     MSIIVVGWNRLIWRPIASHAQTRFGVTQ"
     gene            1783..3147
                     /locus_tag="VFU75_09660"
     CDS             1783..3147
                     /locus_tag="VFU75_09660"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HEU5467146.1"
                     /translation="MSATTAAPTASPPQILLDAQHVSQKFHLPSAGDFEALRDVSLTV
                     KEREVVALVGPSGCGKSTLMRILAGLSRPTSGSVTYRGQPVDGVLSSAAMVFQSFALL
                     PWLTVEQNVAMGLSIGRGDLKPSDRSAVVHAVELVGLSGFEQAYPRELSGGMKQRVGF
                     ARALAVEPEILFMDEPFGALDALTAENLRSQVTELWQDPQTGVSTIIVVTHSVDEAVF
                     LAGRIVVFGSNPGHIREILDNALPYPRDPRSTAFQAMVDRLHAILTATLLPEGERPAA
                     PGVASRLVGFPRVHVPEVLGLLDHLATRNQESGSVFDIAEQLAVDYDRMTSVVRAAEQ
                     LGWVETPGELVKLTPAGRNLVAADTADRTPIVRARVEEHPLFARIVMMLQEAEGRVLD
                     DDEVLADLTIMLPFSKASMVLRTVIEWGRYAGILDHNARDKRLELVQPEGEGAETESP
                     AGEN"
     gene            complement(3144..3602)
                     /locus_tag="VFU75_09665"
     CDS             complement(3144..3602)
                     /locus_tag="VFU75_09665"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEU5467147.1"
                     /translation="MSTTLDARGVVRALRAAHLHDRPRAELLREACERIRAWGPPFTS
                     VYAYMLHGESLVLEAFAGRDTEHTKIAVGVGVCGTAVAEGEDQNVQDVSAVGNYLACN
                     LYTKSELVVLIRRGAAILGQIDIDSDVPAGFGDAEHAAVREVADALAVLL"
     gene            3687..5330
                     /locus_tag="VFU75_09670"
     CDS             3687..5330
                     /locus_tag="VFU75_09670"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HEU5467148.1"
                     /translation="MLSPQTFATHLARAVELFRDPGAKDSQKMELRALMGLLRLSPVV
                     LRAEDGKLSINGMVLEGVMYQSLLDRLAKHQVKEIALPSDAPPAEVFNLLKTLADEPT
                     GDDVPKKLESTGTKRVTVEIARLFEEKEEELPPPPPPPPPPPPSPPPVKHDLGVEGIL
                     QGSLEEMPETARRQSESLSRAVEAPPGALDPRMPPRATPDPQHAPAQAGPPSGPRRGV
                     PSGVDELLARLEQGANLPSAGDTLVLLGEKLEGALKAKRQEEALRIVAGVVRVERLVT
                     DAGPRRQYGIALKRMYTKPLFAILVELLDAPRYAADSELALQRGGGDAVEILLERLAE
                     SPTVAERRRAFDVLARMKEAGSQLINMMGHPQWFVVRNVAELIGEMGLEDAVPALAKK
                     IDHEDVRVRKAVALSLAKIGTRSVVEPLRRALRDPSPEVRTQAALGVGGRRANALAMP
                     LVVALDEEKDLEVSRELILALGRIGSPDAVQALIKLAQSAGGFFSKKPVGLRLAAIEA
                     LRLAATPAALGTLQNLLNDSDRQVKAAAQAAAQEIKGRP"
     gene            complement(5327..6394)
                     /locus_tag="VFU75_09675"
     CDS             complement(5327..6394)
                     /locus_tag="VFU75_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEU5467149.1"
                     /translation="MTQASVAFLKRLLDTPGPSGFESGPARLWRDEVKAFADQVESDV
                     RGNTIAAINPKGQPRLMFAGHIDEIGVQITHIDDDGYCYFAGIGGWDPQVLVGQRITL
                     LGRNGPVLGVVGKKAIHLMKPDERDKVSKITDLWIDIGAAGREEAQARVRVGDPGVLA
                     TDVKEFPNGRIVSRSIDNRIGAYVVAEALRLLAEDRPKHAAVFAVATTQEEIAWTGGG
                     ARTSAVALDPQVALVVDVTHATDYPGAEKKTAGDHKLGGGVVLSRGSATNPKVFDLLV
                     ECAEANKIPFTLQAAPHDTGTDADAIYNAHRGVATGLVSVPNRYMHSPNEMVAMTDLE
                     RAAQLLALFARKLTKETSFIL"
     gene            complement(6394..7386)
                     /locus_tag="VFU75_09680"
     CDS             complement(6394..7386)
                     /locus_tag="VFU75_09680"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEU5467150.1"
                     /translation="MLVLVGLIAGLLAQTPPPHPPPRDTLHILAVGDINLGRKLAKDY
                     LEKGDTLYPFREVLDTFATADLLFGNLESPIAPDAHAVESQGIKFTAPPLAATALARA
                     GFTIVSTANNHAWDGGESGVAQTMNRLSERGVRFVGSGYGREMAEQPVIVERRGWRVA
                     FFAVTRAWNPAPDDFYGHPGSRYVAWGDPNWLYPAIKALKASGLVDLVIVSVHGGTEY
                     QPEPKPSYRHFLEGLADAGADLVLAHHPHVLQPVVFRHGTPIVQSLGNFVFSQDQPWT
                     RLGAILRITVAPDHRIAVRAIPVRAGYQVTFVDGAAADSVRARLRVSLSNRTTP"
     gene            complement(7389..8243)
                     /locus_tag="VFU75_09685"
     CDS             complement(7389..8243)
                     /locus_tag="VFU75_09685"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5467151.1"
                     /translation="MAVPPRVVSLLPAATEIVAALGAGSWLVGVSHECDYPADVTTLP
                     RVTRNTLDPSLPSGAIDAQVAASKHGAPLVTLDTDLLRRLAPDVIFGQSLCDVCAVGE
                     NLVAQALPHLARKPEVVTLHAHTLAEVFDDMMRVAEALDLRGEGEELVAGLRYRLRNI
                     PRPQSPTPPRLLVVEWTDPPYVAGHWVPELVAAAGGADVGSAPGDHSRARPWKDLRAL
                     APDVIVVSLCGFDVPRAKREAAQVHDSDGRALLDGRTFYLDGNAYTSRPGPRLVDAAE
                     LIAKQIGG"
     gene            8266..10266
                     /locus_tag="VFU75_09690"
     CDS             8266..10266
                     /locus_tag="VFU75_09690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HEU5467152.1"
                     /translation="MTVSVVGGTFAHPPTLTAPMADQDSSPYIPASVTLPELTVKALI
                     LGVVLSLLLAGANAYLGLFAGITVSASIPAAVISMAVLRMFRQHNILENNVVQTAAAS
                     GEGLAAGAIFTLPALLILGTWTTFDYWTTTVIAAIGGALGVLFTIPLRRALIIEDPLK
                     FPEGVATAEVLKVGEKGGAGVGTIVAAAGAGALYKLGATGFKLWGEAITSAKYVGGSV
                     AAFAMNLSPALIGVGFIVGLNVAALIFIGGVMNWYVAIPWVASHTAHPDGVAAVDFAA
                     GIWKNQTRYIGVGAMIVGGIWALIKVRKSVVQGIRSGMAAYRHTQAGGTAILRTDRDT
                     PMHWVGIALIVLAVPLFFIFNAFTGIWWVAAAMAVIMLVAGFLFSTVAAYMAGLVGSS
                     NNPISGVTIATILTSALLLLAFLGAGNAKGPAAAVFIGAVVACAAAIGGENLQDLKAG
                     RIVGATPYKQQVMQIIGAVSAAFVLSPVLTLIQKAYGIGVPTADHPHPLAAPQATLMA
                     AVAGGVFHGGLPWTMISIGMVVAVIVISFDLWLQSRQAQFRTPVLAVAIGIYLPLQLS
                     VAIFLGGVVAWLGSRFLRRAGASAEEHATGERNGLLFAAGLITGEALVGILLAIPIVV
                     ASNADVLAYWGEHEGVLPGVILLAAVVYWLYRTATRRERAKA"
     gene            10282..12165
                     /locus_tag="VFU75_09695"
     CDS             10282..12165
                     /locus_tag="VFU75_09695"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HEU5467153.1"
                     /translation="MTDAPDRLFLDFQEVLAGRYSLERELGRGGMGVVYLAREVRLDR
                     LVAIKLLPPELAAVPGLRDRFLREARMAARLSHPNIVPIFAVDEVDRFVFYAMAFVDG
                     ETLAHRVETRGPLPPGEATRLLREVAWALAYAHAQGVIHRDVKPENILLEASGRALVT
                     DFGIARVTRETGATGPGEVIGTPEFMSPEQAVGDTVDGRADLYSLGAVGYFTLQGSPP
                     FSGGSAASLLSQHIATPVPLLEGPGIPRRLARALERCLAKDPAARPQKGEDFAESLGQ
                     VLEAHKELPVALRVFVKRGGRLGGMGGLVYLLFSWAFLGLAVSLAPRGREIMAFWLAF
                     GAAFGVGPLFMLAFRARGFLASGFDPKDLPAAFQAELEQGLEERVFEYGRRASTYERV
                     TGLVAAVGVGTAITAAFMGGLSGFGGSAFVAFSWAALTSITSGFLYLTRLNRRVDLDT
                     KIWSWVWRGPIGRALFAIARPFVGRRSIPAAGTHRATELALSLAAEQLYDALPRATRA
                     RLRELPDALHRLESDAQRMRARLDELQDALGGRDTPAPDPADPMAQRRADVLSALDAE
                     RVLVERRHADAVTALESIRLSLLRLHAGTGTVESLTTDLGLAREVAADVDRLLAAHRE
                     LDG"
     gene            complement(12167..13267)
                     /locus_tag="VFU75_09700"
     CDS             complement(12167..13267)
                     /locus_tag="VFU75_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5467154.1"
                     /translation="MTRLANEHGAINLAQGFPNFPAPDVLKEAAAKAIRDDINQYAIT
                     WGAKRLRNAIAAKYADWYQLAVDPETDVTVTCGATEAMASTLLALINPGDEVIIFEPF
                     YENYGPDAILCGAAPVYVPLLAGQPLDLDRLKAAFSPRTRAVVICTPNNPTGRVLARA
                     ELEAIAELCRKHDAWAVTDEIYEHIYYEGTHIPIATLDGMKDRTITVSGASKTYSITG
                     WRIGTIVAPRQATDAIRKVHDFLTVGAPAPLQEGVAVAIETLGRDYYQTLSREYRARR
                     DLLCGALVEAGFKCTPPEGAYYVLADFSALSPLPDDEFAKRLTAEHGVAPVPGSSFYS
                     RPEMGRKEVRFAFCKTDDMLREAAKRLKKLKD"
     gene            complement(13327..>13546)
                     /locus_tag="VFU75_09705"
     CDS             complement(13327..>13546)
                     /locus_tag="VFU75_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5467155.1"
                     /translation="AGEPYTMEAAMAKLYSSQVAERVTSLSVELFGGYGYTKEYPVEK
                     FYRDAKIGAIYEGTSNMQLNTIAKMLLK"
BASE COUNT         2020 a         4448 c         4698 g         2380 t
ORIGIN      
        1 gcgggggccg caagtaatct tcatcaccat gttggaggcc actgatgtcc tcgacgcccg
       61 acggcgtcgt cccgtcacgg tcgccgacct cgtcgtcctg tgcctcatcg gggcggcgat
      121 cggcggggtg gtcgcattcg cgcgggaatt cggggctcca tacgtccagg cgatccacat
      181 cgatctgtcg cccgcggcgc tgccgaaata cacgctctac tcgctgtcgc gcggcgtctt
      241 cgcgctgatc atttcatacg ggttcgccct ggcattcggt tgggcggcgg cctcgtcccc
      301 gaccgccgaa cggtttctgc tccccctcct cgatattctg cagagcatcc ccgtgctcgg
      361 attcctgccc gggttggtgc tcggattggt cgcgttgttc cccagccgga acgcgggcct
      421 cgagctggcc gcgattctca tgatcttcac cgcgcaggcg tggaacctcg ccctttcctt
      481 ctataactcg ctcaagagcg tgcccgagcc cctgcgggaa gcgtcgcgca tggcagggtg
      541 gagcccctgg cagatcttcc ggaagctcga ggtcccgtcc gcggcgccgg gactcgtgtg
      601 gaacggcatg ctttccatgg cgggcggctg gttcttcctg atggtgaacg aggctttcca
      661 gctcgggaat cgcgactacc gcctgcccgg catcggctcg tacatgagcg tcgcggtgga
      721 gcagggggac cggcgggcca tcatcctggc cttgatcgcc atgatcctca tgattgtcgc
      781 ggtcgaccgc ctgctgtggc gcccggtcgt cgtgtgggcc cagcggttcc ggctcgacga
      841 ctcgagcggg gaaacgacgc agacgtcgtg gctcctggag ttcctgcgcc gttcgcgtct
      901 caggatgcag gcgcacaacg tccgcaagtc ggcggcacgg acggcgcggc gcgcgacccg
      961 gccgctgcgc cggtctctcc gcaccgcgga gcggatactg cgccgggtcc cgcgccccaa
     1021 gccccggcgt gccctcctca tccgcgtcag cggtgcactc ctcttggcgc tggtcctggc
     1081 cggcggggtg tggggtgtct ggcgcctggc cgggctcctc ctccagctat cgggtcccgc
     1141 ctgggtgaac ctcgcggagg ctgccggtct cacctttgtg cgagtgctgg ttgcggtggc
     1201 gctgggcacc gcctgggccc tgcccgcggg catcctcatc ggacgcagta tcaggatcgc
     1261 cgaggtactc cagccggtca tccagatcgt cgcctcgttt cccgcgccga tgatctttcc
     1321 gctcgtggtc gtggggctcg cgctcctcgg ggtgcagctg aatctgggat cgatcgtgct
     1381 catgatcctc tcgtcccagt ggtacatcct gttcaacgtg atctcggccg tcgcggcact
     1441 gccgccccag ctcagcgaag ccgcgcaggt gttcaaggtg tcggggtggc gccggtggcg
     1501 ggccctctat ctcccggcgg cgtttccggc gctcgtcacc ggatggatca cggcggcggg
     1561 cggtgcctgg aacgcgtcga tcgtgtccga gtacatcacc gccgggggca gtctccggag
     1621 caccctgggt ctcgggagct tgatctccga cgcgacggcg cgtgcggact tcccgctgct
     1681 cgcaggcggc attaccctga tgagcatcat cgtggtgggg tggaatcgcc tgatatggcg
     1741 ccccatcgcc tcccacgccc agacgcgatt cggagtgacg caatgagcgc gaccaccgcc
     1801 gctccgacgg cctccccgcc acagattctg ctcgacgcgc agcacgtttc gcagaagttc
     1861 cacctgccca gtgccggtga cttcgaggcc ctccgcgatg tgtctctcac ggtaaaggag
     1921 cgggaggttg tcgcgctcgt aggcccctcg ggatgcggca agagcaccct gatgcgcatc
     1981 ctggccggat tgtcccgccc cacgtccggt tccgttacct accgtggcca gccggtcgac
     2041 ggggtactgt cctcggcggc gatggtgttt cagtcgttcg ccctcctgcc gtggctgacg
     2101 gtggagcaga acgtcgccat gggtctgtcc atcggccggg gggacctgaa gccttcggac
     2161 cgttccgcag tggtgcatgc cgtggagctc gtgggtctct ccgggttcga gcaggcctat
     2221 ccgcgcgagt tgtccggcgg aatgaagcag cgcgtggggt tcgcgcgcgc gctcgccgtc
     2281 gagcccgaga ttctgttcat ggacgagccg ttcggcgccc tcgacgccct caccgcggag
     2341 aacctccgct cgcaggtgac ggagctctgg caggatccgc agacgggtgt ctccaccatc
     2401 atcgtcgtga cgcacagcgt ggacgaggcg gtgttcctcg cgggacggat cgtggtgttc
     2461 ggctcgaacc ccggccacat ccgcgagatt ctcgacaacg cgctccccta tccccgggac
     2521 ccgcgatcga cggcgttcca ggcgatggta gaccggctcc acgccattct gacggcgacg
     2581 ctgctgccgg aaggcgagcg gccggcggca ccgggggtgg cgtcgcggct cgtgggcttc
     2641 ccgcgggtgc acgtaccgga ggtcctgggt ctcctcgacc acctcgcgac acgcaatcag
     2701 gagagcggat cggtgttcga catcgcggaa cagctcgcgg tggactacga ccgcatgacg
     2761 tcggtggtgc gcgcggctga gcagctgggc tgggtggaga cgcccggaga gctggtcaaa
     2821 ctgacgccgg caggccggaa cctggtggcc gcggataccg cggaccgcac gccgatcgtc
     2881 cgggcacggg tcgaggagca tccgctcttc gcgcggatcg tgatgatgct gcaggaggcg
     2941 gagggcaggg ttctggacga cgacgaggtg ctcgcggacc tcaccatcat gctgccgttc
     3001 agcaaggcct cgatggtgct ccggacggtg attgagtggg gacgatacgc cggaattctc
     3061 gaccacaacg cgcgagacaa gcgcctggag ctcgtacagc ctgaaggcga aggggccgag
     3121 accgagagtc cggccggaga gaactagagc agaaccgcga gcgcgtccgc gacttcgcgg
     3181 acggcggcgt gctccgcgtc accgaatccg gcggggacat ccgagtcgat gtcgatctga
     3241 cccaggatgg ccgctccgcg ccggatcagc accacgagct cggacttcgt gtagaggttg
     3301 caggcgagat agttccccac cgccgacacg tcctggacgt tctggtcctc gccctcggcg
     3361 acggccgtcc cgcagactcc gactcccacc gcgatcttcg tgtgctcggt gtctcgcccc
     3421 gcgaacgctt cgagcacgag agactccccg tgcagcatgt acgcatacac cgacgtaaat
     3481 gggggacccc acgcgcggat gcgctcgcag gcctcacgca gcagttcggc gcgcgggcgg
     3541 tcgtgcaggt gggccgcgcg cagcgcgcgg acgacgccgc gggcatcgag ggtggtgctc
     3601 atccccgtaa tctcgcgaga cctgggcact ttggggagcg gcgcgttgcc gctgtttggg
     3661 cgcccgtcta gattgggcga gccctgatgc tgtcacctca gactttcgcc acccacctcg
     3721 ctcgcgccgt ggagctcttt cgagatccag gcgcgaagga ttcccagaag atggaattgc
     3781 gggccctcat gggcctgctg cgtctgtctc cggtggtgct ccgcgccgag gacgggaagc
     3841 tctccatcaa cggcatggtg ctcgaagggg tgatgtacca gagcctgctg gaccggttgg
     3901 cgaagcacca ggtgaaggag attgccctgc cgtcggatgc gccccccgcc gaagtcttca
     3961 atctcctcaa gaccttggcc gacgagccca ccggggatga cgtaccgaag aagctggagt
     4021 cgacggggac caaacgtgtg acggtggaaa tcgcgcggct ctttgaagag aaggaagagg
     4081 agctgccgcc acctccgccg ccccccccac ccccgccgcc atctcctccg ccggtcaagc
     4141 acgacctcgg ggtcgagggg attctgcagg ggagtctcga ggaaatgccg gaaaccgcgc
     4201 ggcggcagtc ggagtcgttg tcgcgcgcgg tcgaagcgcc ccccggggcc ctcgatccgc
     4261 gcatgccgcc ccgcgcgacg cctgatccgc agcacgcgcc agcgcaggcg ggaccgccgt
     4321 ctggtccgcg gcgcggggtg ccgtccggtg tggacgagct cctggcgcgg ctcgagcagg
     4381 gcgcgaatct gccttccgcg ggcgacacgc tcgtcttgct gggcgaaaag ctcgaggggg
     4441 ccctcaaggc gaagcgccag gaggaggccc tccgcatcgt cgcgggtgtc gtgcgtgtgg
     4501 agcgcctggt gacggacgcg ggaccgcggc gtcagtacgg catcgcgctg aagcgcatgt
     4561 acacgaagcc gctcttcgcc attctggtgg agctgctcga tgcgccgcgg tacgcggctg
     4621 actccgagct cgcgttgcag cggggcggcg gagacgccgt cgagatcctc ctggagcggc
     4681 tggcggaatc cccgacggtc gccgagcgcc ggcgcgcctt cgacgtcctg gccaggatga
     4741 aggaggcggg cagccagctc atcaacatga tgggccaccc gcaatggttc gtggtgcgca
     4801 acgtcgccga gctgatcggg gagatggggc tcgaggacgc ggtcccggcg ctggccaaga
     4861 agatcgacca cgaggacgtg cgagtccgga aggcggtcgc cctgtcgctc gcgaagatcg
     4921 ggacgcgctc agtggtggag ccgttgcgtc gcgccctgcg cgatccgtca ccggaggtcc
     4981 gcacgcaggc ggcgctgggc gtcgggggcc ggagagcgaa cgcgctcgcg atgccgctcg
     5041 tcgtggccct ggatgaggag aaggatctcg aagtctcgcg cgagttgatt ctggcgttgg
     5101 gacggatcgg gagtccggac gcggtgcagg cgctgatcaa gctggcgcag tccgcgggcg
     5161 gcttcttcag caagaagccg gtggggctcc ggctcgccgc catcgaggcc ctgcgccttg
     5221 cggccacgcc ggcggcgctc gggactctcc agaacctgct gaacgactcg gacaggcagg
     5281 tcaaagcggc cgcgcaggcg gccgctcagg aaatcaaagg acgaccctaa aggataaagc
     5341 tggtctcctt cgtcagcttc cgcgcgaaga gcgccagcag ctgtgcggcc cgctccagat
     5401 ccgtcatcgc caccatttca ttgggactgt gcatgtagcg gttcggcacc gagaccagtc
     5461 cggtggccac gccgcgatgg gcgttgtaga tggcatcggc gtcggttccc gtgtcgtgcg
     5521 gcgccgcctg cagcgtgaac gggatcttgt tcgcctccgc gcattcgacc agcagatcga
     5581 acaccttcgg gttggtcgcg gatccgcgcg agaggaccac gccgccgccg agcttgtggt
     5641 cgccggcggt tttcttttca gccccgggat agtccgtcgc gtgcgtcaca tccaccacca
     5701 gcgccacctg cgggtcgagc gcgaccgcac tggtgcgcgc gcccccgccg gtccaggcga
     5761 tctcctcctg cgtggtcgcg accgcgaaga ccgccgcgtg cttcggccgg tcctccgcga
     5821 ggagccgcag cgcctcggcc actacgtagg cgccgatgcg gttgtcgatg ctccgcgaga
     5881 cgatgcgacc gttcgggaac tccttcacgt cggtggcaag gacgcccgga tcgccgacgc
     5941 ggacgcgtgc ctgcgcctct tcccggccgg ctgccccgat atcgatccac aggtccgtga
     6001 tcttcgagac cttgtcccgc tcatccggct tcatcagatg aatggccttc ttgccgacga
     6061 cgccgagcac gggaccattc ctgcccagga gcgtgatccg ctggcccacg agcacctgcg
     6121 ggtcccagcc gccgatcccg gcgaaatagc agtagccgtc atcgtcgata tgcgtgattt
     6181 gcacgccgat ttcgtcgatg tgccccgcga acatgaggcg cggttgacct ttggggttga
     6241 ttgccgcgat ggtgttgccg cgcacatcgc tctcgacctg gtcggcgaac gccttgacct
     6301 cgtcccgcca gagccgggct ggtcccgatt cgaatccgga ggggcccggc gtatcgagga
     6361 ggcgcttcag gaaagcgacg gaagcttgcg tcattagggc gtagtcctgt tcgagagtga
     6421 aactcggagc cgcgctcgca cgctgtccgc ggccgcacca tccacaaagg tgacctggta
     6481 tccggcgcgc accggtatgg cgcggaccgc aatccgatgg tccggtgcca ccgtgatcct
     6541 cagaatggct cccagcctgg tccatggctg gtcctgagag aacacgaagt taccgagaga
     6601 ttggacgatt ggcgtgccgt gcctaaacac cactggttga agcacatggg ggtggtgggc
     6661 cagcaccaga tcggcccccg cgtcggccag gccctcgaga aaatgccggt aggaaggctt
     6721 cggttccggc tggtactcgg tccccccatg gacgctcacg atcaccaggt cgaccagccc
     6781 gctggccttg agggccttga tcgcgggata gagccagttg gggtctcccc aggcgacgta
     6841 ccgggatccc gggtgtccgt agaagtcgtc gggtgccggg ttccaggcgc gggtcaccgc
     6901 gaagaacgcg acccgccatc cgcgtcgctc cacgatcacc ggctgctcgg ccatctctcg
     6961 tccgtatccc gagcccacga accgcacgcc ccgctccgaa agccggttca tggtctgcgc
     7021 cacgccgctc tcaccgccgt cccacgcgtg gttgttcgcg gtcgagacga tggtgaagcc
     7081 ggcccgcgcg agtgcggtgg ccgcgagggg gggtgccgtg aacttgatgc cttgcgattc
     7141 caccgcgtga gcgtcggggg cgatggggct ctcgagattt ccgaacagca gatcggcggt
     7201 ggcgaaggtg tcgaggactt cgcggaaggg gtagagcgtg tctcccttct cgagatagtc
     7261 cttcgcgagt ttgcggccaa ggttgatgtc tcccaccgcg aggatgtgca gcgtgtcgcg
     7321 cgggggaggg tgggggggtg gggtttgcgc cagcaggccg gcgatgaggc caaccagaac
     7381 gagcattcct agccgccaat ctgtttcgcg atcagctccg cggcgtcgac cagccgcgga
     7441 cccggtcgcg acgtgtatgc gttgccgtcg aggtagaacg tccgtccgtc gagcagcgcg
     7501 cgaccatcgg agtcgtgcac ctgcgccgct tcgcgcttcg cgcgcggcac gtcgaagccg
     7561 cacaaactga cgactatcac gtcgggcgca agggcccgca aatccttcca gggacgggcg
     7621 cgcgaatggt ccccgggagc ggagccgacg tcggctccac ccgcagcagc gacgagctcc
     7681 ggcacccagt gcccagcgac gtagggggga tcggtccact cgactaccaa gagacgtggg
     7741 ggcgtggggg actgggggcg tgggatgttc cgcagacgat agcggagtcc cgccaccaat
     7801 tcctctcctt cgccgcgcag gtccagtgcc tcggccaccc gcatcatgtc gtcgaacacc
     7861 tccgccaacg tgtgagcgtg aagcgtgacg acttcaggct tgcgggcaag atggggcagc
     7921 gcctgggcca cgaggttctc acccacggcg cagacgtcgc agagcgactg accgaaaatg
     7981 acatcgggcg cgaggcggcg gaggaggtcg gtgtcgaggg tgacgagggg cgcgccgtgt
     8041 ttcgatgccg cgacctgggc gtcgatcgca ccggacggca gcgacgggtc gagggtgttc
     8101 cgggtgaccc gggggagggt ggtcacgtcg gctggatagt cgcactcgtg ggatacgccg
     8161 acgagccagg atcccgcacc cagtgcggcc acgatctccg tggcagccgg aagcagcgac
     8221 acgacgcggg gagggacggc catggcggca atatagaggc cttgcttgac agtctccgtc
     8281 gtgggcggga cattcgcgca ccccccaacc ctgacggcac cgatggcgga tcaagattcg
     8341 tccccttaca tcccggcgtc ggtcaccttg cccgagctca cggtcaaggc cttgatcctg
     8401 ggcgtggtgc tctcgctcct gctcgccgga gcgaacgcgt atctcgggct cttcgccggc
     8461 atcacggtat ccgcttccat tccggcggcg gtcatttcga tggcggtgct gcggatgttc
     8521 cgccagcaca acatcctgga aaacaacgtg gtgcagacgg ccgcggcatc cggtgagggg
     8581 ctcgctgcgg gcgccatctt cacgcttccg gccctcctca tcctgggcac gtggaccacc
     8641 ttcgactact ggaccacgac cgtcatcgcc gccatcggcg gagcgctggg tgtgctgttc
     8701 accatcccgc tccggcgggc gctgatcatc gaggatccgc tcaagttccc cgagggggtc
     8761 gcgacggccg aggtgctcaa ggtcggcgag aaaggggggg ccggcgtagg caccatcgtc
     8821 gcggcggcgg gcgccggggc gctctacaag ctgggcgcga ccgggttcaa gctctggggt
     8881 gaggccatca ccagcgcgaa atacgtcggc ggttcggtcg cggcgttcgc catgaacctc
     8941 tctcccgcgc tgatcggcgt ggggttcatc gtcggattga acgtcgcggc cttgatcttc
     9001 atcggcgggg tgatgaactg gtacgtcgcg atcccctggg tggcgtcgca caccgcgcac
     9061 ccggatggag tggccgcggt cgacttcgcc gccgggatct ggaagaacca gacccgctac
     9121 atcggcgtcg gcgccatgat cgtgggcggc atctgggcgc tgatcaaggt ccggaagagc
     9181 gtggtccagg gcattcgatc gggcatggcg gcgtaccggc acacgcaggc ggggggcacg
     9241 gcgattctcc ggaccgaccg tgacacgccg atgcattggg tggggattgc gctgatcgtg
     9301 ctcgccgtcc cgctcttctt catcttcaat gcgttcaccg gtatctggtg ggtggcggca
     9361 gccatggcgg tcatcatgct ggtcgccgga ttcctcttct ccacggtcgc ggcctacatg
     9421 gcgggactcg tcgggtcctc gaacaatccc atctccggcg tcacgatcgc gactattctc
     9481 acgtccgcgc tattgctcct cgccttcctg ggggccggga acgcgaaggg tcccgcggcc
     9541 gccgttttca tcggtgcggt ggtcgcgtgc gcggccgcga tcggaggcga gaaccttcag
     9601 gatctgaaag ccggccgcat cgtcggggcg acgccgtaca aacagcaggt catgcagatc
     9661 atcggtgccg tgtccgccgc gttcgtcctg tctcccgtat tgaccctgat ccagaaggcg
     9721 tacggcatcg gcgtgcccac ggccgatcac ccgcacccgc tcgcggcgcc gcaagcgacg
     9781 ttgatggccg cggtggcggg aggcgtgttc cacggcggtc ttccctggac catgatcagc
     9841 atcggtatgg tggtcgcggt gatcgtgatc agcttcgatc tctggctcca atcgagacag
     9901 gcccagttcc gcacgccggt gctggccgtc gcgatcggga tctacctgcc gctgcagctt
     9961 tccgtcgcga tcttcctggg gggcgtcgtc gcgtggctgg gaagccgctt cctcaggcgg
    10021 gcgggcgcgt ctgctgagga gcatgcaact ggagagcgga acggcttgct cttcgccgcc
    10081 ggcctcatca cgggagaagc gctggtcggc atcctcctcg cgattccgat cgtggtggcg
    10141 tccaacgccg acgtgctcgc gtactggggt gagcacgaag gggtgcttcc cggcgtcatc
    10201 ctgctcgccg ccgtagtcta ctggctctat cgcaccgcta cccgcaggga gcgcgcgaaa
    10261 gcgtagggcg ctgcaagacg catgaccgac gccccggatc ggctgtttct ggatttccag
    10321 gaagtcttgg cagggcggta ctccctggag cgggagctcg gccggggcgg catgggtgtc
    10381 gtgtacctcg cgcgcgaggt gcgactggac cgcctggtgg ccatcaagct cctgcccccg
    10441 gagctcgccg cggtccccgg gctgcgcgat cgcttcctcc gagaggcgcg catggcggcg
    10501 cgcctctccc accccaacat cgtgccgatc ttcgcggtcg acgaagtcga ccggttcgtc
    10561 ttttacgcca tggcgtttgt ggacggggag acgctggcgc atcgggtgga gacccgaggc
    10621 cccctgcctc ctggggaggc cacccgtctg ctccgagagg tcgcttgggc cctggcctac
    10681 gcgcacgcgc agggagtcat tcatcgcgat gtgaaacccg aaaatattct gctcgaagct
    10741 tcggggcggg cgctggtcac cgacttcggg attgcgcgcg tgacgcggga gaccggcgcc
    10801 acgggaccgg gcgaagtgat tgggacaccc gagttcatga gtcccgagca ggccgtgggc
    10861 gacaccgtcg acggccgcgc ggatctctac tccctcggcg ccgtcgggta cttcacgttg
    10921 caggggagcc cgccgttctc gggtgggagc gccgcatcct tgttgtcgca gcacattgcg
    10981 acccccgtgc ctctgctcga aggaccaggg atcccccgcc gactggcccg ggcgctcgag
    11041 cgctgcctcg ccaaggatcc agcggcccgg ccacagaagg gtgaggactt cgccgagtcg
    11101 ctggggcagg tactcgaggc gcacaaggag ctcccggtgg ccctccgagt cttcgtcaag
    11161 cgcggcgggc gcctcggagg catgggcggg ctcgtatacc tcctcttctc gtgggctttc
    11221 ttgggcctgg cggtgagcct ggcaccgcgc ggtcgcgaga tcatggcgtt ctggctcgcg
    11281 ttcggcgccg cgttcggcgt gggccctctg ttcatgctgg ccttccgggc tcgcggtttc
    11341 ctcgcttctg gattcgatcc caaggatttg cccgcagcgt tccaggccga gctggagcag
    11401 ggcctcgagg aacgggtgtt cgaatatggg cgacgggcgt cgacctacga acgagtcacc
    11461 ggcctggtcg cggcagtggg agtgggtacc gccatcaccg ccgccttcat gggcggcctc
    11521 tcgggatttg gcggcagcgc gttcgtggcg ttcagttggg ccgcattgac gtcgattacc
    11581 agcggattcc tctatctgac gcgtctcaac cgccgtgtgg acctggatac caagatctgg
    11641 tcttgggtgt ggcggggacc gatcggccgt gcgctgttcg ccatcgctcg acccttcgtc
    11701 ggacgccgat ccattcccgc cgccggtacc caccgggcga cagagctcgc gctgtcgctc
    11761 gccgccgagc agctctacga tgcgttgccc agggccacgc gcgcgcggct ccgcgagctc
    11821 ccggacgcgt tgcaccggct cgaaagcgac gcgcaacgga tgcgggcacg gctcgatgag
    11881 ctccaggacg ccctgggggg gcgagacaca cctgcgcccg atcccgccga tcccatggct
    11941 cagcgaaggg ccgacgtgct gagcgcgctc gatgcggaac gagtgctagt ggaacggcgg
    12001 catgcggacg ccgtcaccgc gctggaatcg atccgcctga gtctgctgag attgcacgct
    12061 ggaaccggga cagtggagag cctgacgacg gatctgggac tggcacggga agtggccgcg
    12121 gatgtggacc gcctgctggc cgcccaccgg gagctggacg ggtgatctaa tccttcaact
    12181 tcttcaaacg cttcgctgcc tccctcagca tgtcgtcggt cttgcagaac gcgaaccgca
    12241 cttccttcct tcccatctcc ggcctgctgt agaaactcga gcctggcacc ggcgccacgc
    12301 catgctcggc cgtgaggcgc ttcgcgaatt cgtcgtccgg caagggggaa agggcggaga
    12361 agtcggcaag cacgtagtac gccccttcgg gcggcgtgca cttgaacccc gcctccacca
    12421 gcgcgccgca gagcaggtcg cgacgagccc ggtactcccg ggagagcgtc tggtagtagt
    12481 cgcgtcccaa cgtttcgatc gcgacggcaa cgccctcctg cagcggcgcc ggagcgccca
    12541 cggtgagaaa atcgtggact ttccggatcg cgtcggtcgc ctggcgcggc gccacgatcg
    12601 tcccgatgcg ccacccggta atcgagtacg tcttggaggc tccgctcacc gtgatcgtcc
    12661 ggtctttcat gccgtcgagc gtggcgatgg ggatgtgcgt gccttcgtag tagatgtgct
    12721 cgtagatctc atccgtcacc gcccaggcgt cgtgttttcg gcagagctcg gcgatggcct
    12781 cgagctccgc gcgggccagc acccggccgg tggggttgtt tggtgtgcag atcacgaccg
    12841 cgcgcgtgcg cggagagaag gcggccttca ggcggtcgag atcgaggggc tgcccggcga
    12901 gcagggggac gtagacaggc gccgcgccgc agagaatggc atcgggcccg taattctcgt
    12961 aaaagggctc gaagatgatc acctcgtccc cgggattgat cagggccagg agggtcgagg
    13021 ccatcgcttc ggtggcgccg caggtgacgg tgacgtcggt ctcggggtcg acagcgagct
    13081 ggtaccagtc tgcatacttg gcggcgatcg cgttgcggag ccgtttggcg ccccaggtga
    13141 tggcgtactg attgatgtcg tcgcggatcg ccttcgcggc cgcttctttc agcacgtccg
    13201 gcgcggggaa gttcgggaat ccctgcgcga ggttgatggc cccgtgctcg ttggcgagcc
    13261 gggtcatgcc tcggatgacg gattcagtga aggagtgggt acggcgggct gtcgggagcg
    13321 taccgcctac ttcagcagca tcttggcgat ggtgttgagc tgcatgttgc tcgtgccttc
    13381 gtagatggcg ccgatcttcg catcccggta gaacttctcc accggatatt ccttggtgta
    13441 gccgtagccg ccgaacagct ccacggagag cgaggtgacg cgctcagcga cctgggacga
    13501 gtaaagtttg gccatcgccg cttccatcgt gtacggctca ccggcg
//
LOCUS       DASUBP010000258         5411 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_826847, whole genome shotgun sequence.
ACCESSION   DASUBP010000258 DASUBP010000000
VERSION     DASUBP010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5411)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5411)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5411
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826847"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="VFU75_09710"
     CDS             complement(<1..180)
                     /locus_tag="VFU75_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEU5467156.1"
                     /translation="MRYRVVGAGVPVVLLHGYANSLEGWSGLGDSLARDHEVIALDLR
                     GHGQSTKFTRQSDYGR"
     gene            584..928
                     /locus_tag="VFU75_09715"
     CDS             584..928
                     /locus_tag="VFU75_09715"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU5467157.1"
                     /translation="MADKVDVKQGTLALMILTTLQGLGPLHGYGIARRIEETSGHRLK
                     LNYGTLYPALLKLEQEGFITAEWRQSENNRRAKFYALSAAGRKRLAHEEREWRRTTDL
                     IAAFLAPQRETP"
     gene            925..3627
                     /locus_tag="VFU75_09720"
     CDS             925..3627
                     /locus_tag="VFU75_09720"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5467158.1"
                     /translation="MSTLRALFARIASLFARQKSDDDLRDELESHIEMETAENIRRGF
                     APEEARRQARLASGGVTQAAEAVRDRRGIPWIESAVADLRYAVRALRHSPAFVTVVVI
                     TLGLGIGANTAIFSVVRGVLLKPLPHRDGQRLVYLRQSMDGPAGDNVDFSVPEIADLR
                     AATPAFGGIAEYSPWNVIIEGEHDASRIDVGLVTGNFFDVMGLSAVIGRVTRPSDDGP
                     GVPAVMVLTHEFWMRRFGGDPSIVGKQLRLDGGSATVIGVLQPAPFFPGPMDALMNMV
                     VSPHHLSAQMVQGRTHRMTEILARLAPGATLDQARGQVAAAYGRMQHDHPDAYDPGSH
                     YRVSVIPFKEALGDRARATLWLLMAVAAFVMIIAVANVVNLTLMRGVRRERELVVRAA
                     LGAGVLRLRRLLLAENFVITVVGALFGVVLAIGGVRLLVALAARYSPRANEIRLDPVT
                     LGFALGLSVAVALLLSFVAALPREGTLGSWISAAAGRLSGGLRKQRLQRALVVAQIAV
                     SVVLLAGAGLLTRTMIRLADVDTGLKTEQVLAIDVPLLSPSQLSGTASILQADAAAKA
                     NYERMRGEIASIPGVVDVGLGSTMPLSLALGSLDVKAEGRTPAVGEAVPHADFRTADP
                     DYFHAAGIPLLAGRTFTLTDRIGSGRVVIINRVLADRLFPGEDPVGRRVAWTGDVLRF
                     TPFSGDWRTVVGVVGNTRDGGLDAPPVPAMYMPFASEVAFTGDLVVRADTDVSGLVAS
                     ATAVVRRLAPTTPIDHVLTIAQIKDESVAPRRLNAALVSSFGLLAVLIAAVGIAGVLA
                     FSVSSRTNEIGIRMSLGADQGQVLRMILGEGGFLLALGLGLGLTGAFVGTNLIQGLLF
                     GVSPRDPLTLLGVALLMAVIGIVACWIPAARAARVDPVAAMRAT"
     gene            complement(3645..>5411)
                     /locus_tag="VFU75_09725"
     CDS             complement(3645..>5411)
                     /locus_tag="VFU75_09725"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5467159.1"
                     /translation="AEISALARQIRNASTEQGDYLLKDGMVMLLRTALTGDARQPLFI
                     LLGAVGFLLLVACANVANLLLAQATARERELALRSALGAGRGRLVRQLLTETLILSVT
                     GGGLGVLAAYWGVARLVALAPANLPRLDEVSINGAVLAGALVLATLVAAGLGVFVASR
                     ATSTDLRGTLVEGGRGSAGSRGTQRAGRVIVAAQIAITMVLVVGAGLLGRSLLKVLAV
                     NPGFRVDRIVTMDVSLPWVSDPAVRARQGRFYADLIGRLERIPGVEGIGAASALPMDG
                     GLPDGMFVEMNPGANPKTFDDLRPLFAQKELQGNADFAVATAGYFHVLGIPLIEGRLF
                     DDRDGPDAPHVAVISEALARLRWPNQDPLGRVIEFGNMDGDLRVLTIVGVVGDTHEDG
                     LERPPAPTVYVNLYQRPRAVMTVAMASTADPQLITTAARQILHDADPELPPRFRTLSQ
                     VYSASLGSRRFNVILTAAFGLTALILATAGVFGVMAYAVSRRTREIGVRVALGAASAD
                     VLRMILGQGARTIGVGVGVGVAISLALTRTVQSLLFGVTATDPVTFGVVTVLLVGAAL
                     AACYIPARRATKVDPMIALRAD"
BASE COUNT          885 a         1880 c         1796 g          850 t
ORIGIN      
        1 tcgtccgtaa tcgctctgtc gcgtgaactt ggtgctttga ccgtggccac gcagatccag
       61 cgcgatcacc tcatgatcgc gggccagcga atcaccgaga cctgaccaac cctcgaggct
      121 gttcgcgtag ccgtgaagca ggactacggg cacccccgcc cccacgacgc gataccgcat
      181 cctcaccgta ccgagttgcg cgtactgatc cgccgcagtc gcaatcgtga cggccggggc
      241 ggccgtttgg ataggcgggc gcgcacctgc cagtccgacc acaaggatga caggccaaca
      301 cagtgagcga cggcgcatac gacctccaac tcttggggaa gctggctcac gtcatggatc
      361 acatcggtgg acgacgcacc gtaggagctg cgtcacggcg ctacaaggcc gcaacggcga
      421 atcgctgccc gggccggatg acaggtattg agcgcaggct tgggtggcag cccttcggct
      481 cctcgccaga ccggagtggt gggacgaccc ctcgcctcga aatggacttg tgaaactccg
      541 gccctggtga tagcgtcata tgggaatccc ataggacggc cagatggcgg acaaagtcga
      601 cgtcaaacag ggcacgctcg ccctgatgat tctcaccacc ctgcaggggc tcggccccct
      661 gcacgggtac ggcatcgcgc gccgcatcga agagaccagc ggccaccgcc tcaagctcaa
      721 ctacggcacg ctctacccag ccctcctgaa gctcgagcag gaagggttca tcacggccga
      781 atggcgccaa tctgaaaaca accggcgcgc gaagttctac gcgctgagcg ccgcgggtcg
      841 gaagcgcctc gcccacgaag aacgggaatg gcgccggacg actgacctga tcgcggcgtt
      901 cctggcgccc caacgggaga ctccatgagc accctgcgtg cactcttcgc ccgcatcgcg
      961 agcctcttcg cccggcagaa gtccgacgac gatctgcgcg acgagctcga gtcccacatc
     1021 gagatggaga ccgccgagaa tatccggcgc ggattcgcgc ccgaagaagc ccgccgtcag
     1081 gctcggctcg catcgggcgg ggtgacgcag gccgccgaag cagtccgtga ccggcgcggc
     1141 atcccgtgga tcgagagcgc cgtcgccgac ctccggtacg cggtccgcgc gctccgccac
     1201 agccccgcct tcgtcaccgt ggtggtcatt accctcggcc tggggatcgg cgccaacacc
     1261 gcgatcttca gcgtggtgcg cggcgtgctg ctcaagcctc tgcctcaccg cgacgggcag
     1321 cgcctggtct acctccggca gtccatggat gggccggcgg gtgacaacgt cgacttctcg
     1381 gttccggaga tcgcggacct ccgggcagcc acaccagcat tcggcggcat cgccgagtac
     1441 tccccgtgga atgtcatcat cgagggggag cacgacgcca gccgcatcga tgtcgggctg
     1501 gtcaccggca acttcttcga cgtgatgggg ctctcagcgg tcatcggccg ggtcacccgt
     1561 ccctcggacg atggccccgg cgttccggcc gtgatggtcc tgacgcacga attctggatg
     1621 cgccgcttcg gcggtgatcc gagcatcgtc gggaagcaac tccggctgga tggcggatcg
     1681 gcgacggtga tcggcgtgct ccaacccgca ccgtttttcc ccggcccgat ggacgccctc
     1741 atgaacatgg tggtcagtcc gcaccatctc agcgcgcaaa tggtgcaggg ccgcacccac
     1801 cgcatgaccg aaatcctggc gcgcctcgcg cccggagcca cgctggacca ggcccgaggc
     1861 caggtggccg cggcgtacgg gcggatgcag cacgatcatc cggatgcgta cgacccgggg
     1921 tcgcactatc gcgtctccgt gatcccgttc aaggaggcgt tgggagaccg cgcccgggcc
     1981 acactctggc tcctgatggc cgtcgccgcc ttcgtcatga tcatcgcggt agcgaacgtc
     2041 gtgaatctga cgctcatgcg gggcgtccgt cgggaacgcg agctggtcgt ccgcgcggcc
     2101 ctcggcgccg gcgtgctccg gctccggcgg ttgctcctgg ccgagaactt cgtgatcacc
     2161 gtcgtcggag cgctgttcgg ggtggtgctg gcgatcggcg gcgtgcggct gctggtcgcg
     2221 ctggcggcgc ggtattctcc ccgcgcaaac gaaatccggc tcgacccggt gaccctgggc
     2281 ttcgctctcg ggctgtcggt ggcggtggcc ctgctcttgt cgttcgttgc cgcactcccg
     2341 cgggagggca cactcggatc ctggatctcc gctgccgcgg ggcgactcag tggagggttg
     2401 cgcaagcagc ggctgcaacg ggcactggtc gtggcgcaga tcgccgtttc cgtggtgctg
     2461 ctggcgggtg ccggactgct cacccggacc atgatccgtc tggcggacgt ggacaccggc
     2521 ctcaagaccg agcaggtgct ggcgatcgac gtgccactgc tcagcccctc acagctgtcg
     2581 gggacggcca gcatcctcca ggccgatgcc gcggccaaag cgaactacga gcgtatgcga
     2641 ggcgagatcg cctccattcc cggcgtggtg gacgtggggt tggggtccac catgcccctc
     2701 agcctcgcct tgggcagtct cgacgtcaaa gcggaaggcc ggactccggc cgtgggcgaa
     2761 gcggtaccgc acgccgactt ccggaccgcc gatcccgact atttccacgc ggcgggtatc
     2821 ccactcctgg cgggtcgcac ctttaccctg accgaccgga tcggcagcgg gcgcgtcgtg
     2881 atcatcaatc gggtgttggc cgaccggctg ttcccgggcg aggaccccgt gggccggcgg
     2941 gtcgcctgga ccggtgacgt tctgcgattc accccgttca gcggggattg gcgcacggtg
     3001 gtgggcgtgg tcggaaacac gagggatggg ggcctcgatg cgcctccggt gccggcgatg
     3061 tacatgccgt tcgcttccga ggtggccttc accggtgacc tggtggtgcg agccgatacg
     3121 gatgtgtccg ggctcgtagc gtcggcgacg gccgtcgtcc gtcggctggc gccgaccacc
     3181 cccatcgacc acgtcctgac cattgcgcag atcaaggacg agagcgtcgc gccgcgccga
     3241 ctcaacgccg cgctggtctc gtcgttcggg ctcctggccg tgctgatcgc ggcggtgggg
     3301 atcgccgggg tactcgcctt ctcggtcagc tcgcgcacga acgaaatcgg gatccggatg
     3361 agcctggggg ccgatcaggg ccaggtgctg agaatgatcc tgggagaagg cgggttccta
     3421 ttggccctcg gcctcgggct cggcctgacg ggagcgttcg tcggcaccaa cctgatccag
     3481 ggcctcctgt tcggcgtcag cccgcgggat ccgttgacac tcctcggcgt cgcactgttg
     3541 atggcggtga tcggcatcgt cgcctgctgg atccccgcgg cgcgagccgc ccgcgtcgac
     3601 cccgtggccg ccatgcgggc gacctagcca gcggggctga tcgactagtc ggctcgcagc
     3661 gcgatcatcg ggtccacttt cgtcgcccgg cgcgccggga tgtagcaggc ggccagcgcg
     3721 gcgcccacca acagcacagt gaccacgccg aaggtcaccg gatccgttgc cgtgacgccg
     3781 aaaagcaacg actgcacggt gcgggtcagc gcgagggaga tggcgactcc cacccccacc
     3841 ccgaccccga tggtcctggc gccctgcccg agaatcatcc tgagcacgtc cgcggatgcc
     3901 gcgcccagcg ccacccggac gccgatctcc cgcgtgcgcc ggcttaccgc gtacgccatg
     3961 accccgaaca ccccagccgt cgcgaggatg agcgcggtca atccgaacgc cgccgtcagg
     4021 atgacgttga agcgccggga gccgagtgac gcggagtaca cctgcgaaag cgtgcgaaat
     4081 cgcggtggaa gctcgggatc ggcgtcgtgc aggatctgcc gagccgctgt cgtgatcaac
     4141 tgcggatcgg cggtgctcgc catcgccacg gtcatcaccg cgcggggacg ctggtacaga
     4201 ttcacgtaga ccgtcggcgc cggaggacgt tcgagcccgt cttcgtgggt atccccgacg
     4261 actccgacga tggtcaaaac ccggagatct ccatccatgt tcccgaactc gattacccgg
     4321 ccaaggggat cctgattcgg ccaccgcaac cgggccagcg cctcgctgat taccgccacg
     4381 tgcggtgcat caggcccgtc gcgatcgtcg aatagacggc cctcgatcag cggaatcccg
     4441 agcacatgga aatagccggc cgtcgccacg gcaaagtccg cgttcccctg cagctccttc
     4501 tgtgcaaaga gcgggcgcaa gtcgtcgaag gtcttgggat tcgccccagg attcatttcc
     4561 acgaacatgc cgtccggcag accgccatcc atcggcagcg cactcgccgc gccgattcct
     4621 tcgacacccg gaatccgctc cagccggccg atcaggtcgg cgtagaatcg cccttgccgg
     4681 gcgcgtaccg cgggatccga cacccacggc agcgagacgt ccatcgtgac gatgcggtcc
     4741 acgcggaagc ccggattcac cgccagtacc ttgaggagac tgcggccgag caggcccgct
     4801 cccaccacga gcaccatcgt gatggcgatc tgagcggcca cgattactcg acctgcccgc
     4861 tgcgtgccgc gggacccagc cgagccgcgg cccccctcga ccagtgtccc gcgcaggtcc
     4921 gtcgacgtcg cacgggaggc cacgaacact ccgaggcctg ccgctacgag ggtcgcgagc
     4981 accagtgcgc cggccaggac ggccccattg atcgagactt cgtcgagtcg tggcaagttg
     5041 gcaggcgcca aagcgaccag gcgcgcgaca ccccagtatg ccgccagcac gcccaggccc
     5101 cctccggtca cggacaagat cagcgtttcc gtcagaagct ggcgcaccaa gcgcccgcgt
     5161 ccggcgccga gggcgctgcg aagggcgagt tcgcgctccc gcgcggtcgc ttgggccagc
     5221 aggaggttgg cgacgttggc gcacgcgacg agcaggagaa agcccaccgc ccccagaaga
     5281 atgaagagcg gttgccgcgc gtccccggtc agtgcggtcc ggaggagcat caccatcccg
     5341 tccttcagga gatagtcgcc ctgttcggtg gaagcgttgc gtatctgacg cgcgagcgcg
     5401 gagatctccg c
//
LOCUS       DASUBP010000259         4421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1153768, whole genome shotgun sequence.
ACCESSION   DASUBP010000259 DASUBP010000000
VERSION     DASUBP010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4421
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153768"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1878)
                     /locus_tag="VFU75_09730"
     CDS             complement(<1..1878)
                     /locus_tag="VFU75_09730"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HEU5467160.1"
                     /translation="MRRAIFFTAILISGAAGSAYGQFAPAGTGGVAALADDLRRLGAT
                     KRVLVIGAHPDDEDTQLLAYLSRGLGVDAAYLSLTRGEGGQNVIGEELGPELGIIRSE
                     ELLAARRLDGAHQYFTRAYDFGFSKNIDEAFRFWPRDSVLKDVIAVIRRFRPQVIVSI
                     FTGTPRDGHGQHQVAGAVARQAFDILKDSSWGPVKFYRSSFFDTAGTTLRMAAGTLDP
                     LVGESYFQIAMAGRSQHRSQDMGQIQRPGPSQIRLTLIATKKPSSGADGFFTGVDTTL
                     HGHDAYVALVDSARRRLSPYDPGAVAPVLARALEALGTDDPDQRALAESALADAAGVV
                     VDGIADDDIVIRGERVQVEADLWNAGNDSVRMTGVDFSVPPAWSVESMETGGATIPTG
                     SVVQRHFAVHVAADAPRSQPYFLEKPLSGALYDWSGVSADIRGLPFGPPPVSMRVHLV
                     IDGAPVTLTREVVYRFRDQARGEVRRPLLVTEPFEVAITPGMVVWPQTAGGSRQFTVQ
                     VTNRNRGPATGRLDIAVPTGWAPVPADSLSFTREDETKSYTFTVDLPPNTKPGDFRLH
                     ATVRGADRTISDGALAVIDYPHIEPRGVVHPSVVEIKVAPIALPALSRIGYIRGASDR
                     VP"
     gene            2015..3874
                     /locus_tag="VFU75_09735"
     CDS             2015..3874
                     /locus_tag="VFU75_09735"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5467161.1"
                     /translation="MTTATEPKTGVSAAEARDVAEAARETEWTGRGFVRDLFLGSLQM
                     DLIHPYPVASADEDKRAEPFLDGLKKFLKHNVDGDKIDREGEIPPAVIEGLRQMGAFG
                     IKIPREYGGLGLSQLSYMKAMELVSTVDGSVTALLSAHQSIGVPQPLKMFGSEAQKKK
                     YLPRLAKGSISAFALTEANVGSDPTNVETHAEPTDDGEAFILNGEKMWCTNGTIAELM
                     VVMARTPSKVVNGKEKRQITAFIVEADSPGVKIEHRCHFMGLRALYNGVISFKNVRVP
                     KENVLWGEGKGLKLALTTLNTGRLTLPISAVAVGKRCLEISRQWSNERVQWGLPIGKH
                     DAIAQKIGTMAANTFAMEAVAELCGAMADRGGYDIRLEAAIAKLYNSEAGWRIIDDTV
                     QIRGGRGYETADSLKARGETNWPVERIMRDFRINLIFEGSSEIMHLFIAREAVDKHLQ
                     TAGDVVMPGKSIGQRLAGLWRSALFYGWWYPSRWIGWSFWPKYGEYGALAKHVRFVER
                     ASRRLARGVFHSMIRFGPKLEQRQSVLFRLVDVGAELFAMSAACARVQMLKKRDQASG
                     VRAERLADLFCRQARERIRVKFQEMWNAADVPTYRTAQEVLKGEHLWLEKGLV"
     gene            complement(3894..4301)
                     /locus_tag="VFU75_09740"
     CDS             complement(3894..4301)
                     /locus_tag="VFU75_09740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HEU5467162.1"
                     /translation="MKPHERLIAWHHCHRLALVTYRITRTFPKTEEYGMTSQMRRAAS
                     SAAANLVEGAAKRGSREFRRFIDTALGSLAEVSYFALLAKDLELLSQEQWEELSQVSD
                     EAGRTTMGLYKAIARRAANGGRVNGKESVSLAV"
BASE COUNT          804 a         1438 c         1454 g          725 t
ORIGIN      
        1 gggcacccgg tcggatgcgc cgcggatgta gccgatgcgg gaaagtgccg gcagcgcgat
       61 cggcgccacc ttgatctcca ccaccgacgg atgcaccacg cccctcggct cgatatgcgg
      121 gtagtcgatg acggccagag cgccatccga gatggtgcgg tcggcgccgc gcaccgtcgc
      181 gtggagacgg aaatcccccg gcttggtgtt cggcggcaaa tcgacggtga aggtgtagct
      241 cttggtctcg tcctcgcggg tgaacgacaa cgaatcggca ggcaccggcg cccatcccgt
      301 cgggacggcg atatcgagcc gtcccgtggc cgggccccga ttccggttcg tcacctggac
      361 cgtgaactgg cgggagccac cagcggtctg cggccacacc accatccccg gcgtgatcgc
      421 cacttcgaac ggctccgtga ccaggagcgg acgccgcact tccccgcggg cttgatcgcg
      481 gaagcgatag acgacttccc gggtgagcgt tacgggagca ccgtcgatca ccagatgcac
      541 ccgcatggag acgggcggcg gcccgaacgg caatccccgg atgtccgccg agactcccga
      601 ccaatcgtag agtgcgccgc tcaggggttt ctcgaggaag tacggctgcg agcgaggggc
      661 gtcggccgcg acgtgcacgg cgaaatggcg ctgcaccacg gaacccgtgg ggatcgtcgc
      721 gccgccggtc tccatcgatt ctacggacca cgccgggggt accgagaaat cgacgccggt
      781 catccggacc gaatcgtttc ccgcgttcca gagatccgcc tccacctgca cccgctcgcc
      841 gcggatcacg atgtcgtcgt ccgcaatgcc gtccaccacc acaccagcgg catccgccag
      901 cgcgctctcc gccagcgccc gctggtcggg atcgtccgtg ccgagcgctt cgagcgcgcg
      961 cgcgagcaca ggtgccaccg ccccgggatc gtagggcgac aggcggcgac gcgccgaatc
     1021 cacgagcgcg acgtaggcat cgtgcccgtg cagcgtcgtg tcgacgccgg tgaagaaccc
     1081 gtccgcaccc gacgacggct tcttcgtggc gatcagggtc agccggatct ggctcggtcc
     1141 cgggcgctgg atctgcccca tgtcctgcga ccggtgctgg ctgcggcccg ccatcgcgat
     1201 ctggaaatac gactccccca cgagggggtc gagcgtcccc gcggccatcc gcaacgtcgt
     1261 gccggcggta tcgaagaagg acgagcggta gaacttcacc gggccccagc tcgagtcctt
     1321 gaggatgtcg aacgcctgcc gggcgaccgc gcccgcgact tgatgctgcc catgtccgtc
     1381 gcgcggcgtg ccggtgaaga tcgagacgat cacctggggc cggaagcgcc tgatgacggc
     1441 gatcacgtcc ttgagaacgg agtcccgcgg ccagaagcgg aaggcttcgt cgatgttctt
     1501 ggagaatccg aagtcgtagg cccgcgtgaa gtactggtgc gcgccgtcga ggcgacgggc
     1561 cgcgagcagc tcctcgctcc ggatgattcc gagctcgggc ccgagctcct cgccgatgac
     1621 gttctgcccg ccctccccac gcgtgagcga cagataggcc gcgtcgacgc cgagtccacg
     1681 ggagaggtac gcgaggagct gggtatcctc gtcgtcaggg tgggcgccga tgaccagcac
     1741 ccgcttggtg gcgccgaggc gccggaggtc gtccgccaac gcggcgacgc cgccggtgcc
     1801 ggccggagca aattgcccgt atgccgaacc tgcggcgcca ctgatcagga tggcggtgaa
     1861 gaagatcgct cgtcgcattt atggaagata cctcgggacg cccttcataa cgaatttacc
     1921 gcgcgggggt tccgtcatcg gttcgtcccc aatctgaacc ggttcacccc gccccattcc
     1981 cgccctgggg gtatagattg aggaaggaga ccccatgacg actgcgacag agcccaagac
     2041 cggcgtatcc gctgccgaag cccgagacgt cgccgaggcg gcccgcgaga ccgagtggac
     2101 cggacgtggt ttcgtccggg acctgttcct cggctccctc caaatggacc tgatccaccc
     2161 ctaccccgtg gcgtccgccg acgaagacaa gcgggccgag ccgtttctgg acggcctcaa
     2221 gaagttcctc aagcacaacg tcgacgggga caagatcgac cgcgagggcg agatcccacc
     2281 cgcggtcatc gagggcctgc ggcagatggg cgccttcggg atcaaaatcc cgcgcgagta
     2341 cggcgggctg ggcctctccc agctctccta catgaaggcg atggagctgg tgtcgaccgt
     2401 cgacggctcc gtcaccgcgc ttctctcggc acaccaatcc atcggggtgc ctcagccgct
     2461 caagatgttc ggctcggagg cgcagaagaa gaagtacctg ccccgtctgg ccaaggggtc
     2521 gatctccgcc ttcgcgctca ctgaggccaa cgtgggatcc gatccgacca acgtcgaaac
     2581 gcatgccgaa cccaccgatg acggcgaagc cttcattctg aacggcgaga aaatgtggtg
     2641 caccaacggg accatcgcgg agctgatggt ggtgatggcc cggacgccct cgaaggtcgt
     2701 gaacggcaaa gagaagcgcc aaatcaccgc gttcatcgtc gaagccgatt ccccgggcgt
     2761 gaagatcgag caccggtgcc atttcatggg gctgcgggcg ctctacaacg gcgtgatcag
     2821 cttcaagaat gtccgcgtcc ccaaggagaa cgtgctgtgg ggtgaaggca agggcttgaa
     2881 gctcgccttg accacactga acacgggccg cctcaccctg cccatctccg ccgtcgccgt
     2941 gggcaagcgc tgtctcgaga tctcccggca gtggtcgaac gagcgggtgc agtggggact
     3001 gccgatcggc aagcatgacg ccatcgccca gaagatcggc acgatggccg cgaacacgtt
     3061 cgcgatggag gcggtggccg agctgtgcgg cgccatggcg gaccgcggcg gctacgacat
     3121 ccgcctggaa gcggcgatcg ccaagctcta caactcggag gcgggttggc ggatcatcga
     3181 cgacacggtg cagatccggg gcgggcgcgg gtacgagacc gccgattcac tcaaggcccg
     3241 gggggagacc aactggccgg tcgagcggat catgcgggac ttccggatca atctcatctt
     3301 tgaaggctcc agcgaaatca tgcacctgtt catcgcccgt gaggcggtgg acaagcacct
     3361 tcagaccgcg ggcgacgtgg tgatgcccgg caagtccatc ggccagcgcc tcgccgggct
     3421 ctggcgctcc gcgctcttct atggctggtg gtatcccagc cgctggatcg gctggagttt
     3481 ctggcccaag tacggcgagt acggggctct cgcgaagcac gtccgcttcg tcgaacgcgc
     3541 ctcccgccgc ctcgcgcgcg gcgtgttcca ttcgatgatc cgcttcgggc cgaagctcga
     3601 gcagcggcaa tccgtgctct tccgtctcgt ggacgtcggt gccgagctgt tcgcgatgtc
     3661 ggcggcgtgc gcgcgggtgc agatgctgaa aaagagggat caggcgtcag gggtcagggc
     3721 ggaaagactg gccgatctgt tttgccggca ggcccgcgag agaatcagag tcaagtttca
     3781 ggagatgtgg aatgcggctg acgtaccgac gtacagaact gcacaggaag tcttgaaagg
     3841 tgaacatctg tggctggaga aggggctggt ctgacaagta gacggttaaa cggttagacg
     3901 gctagggaga ccgactcttt cccattcacg cgcccgccgt ttgccgcccg gcgggcgatt
     3961 gccttgtaga gccccatggt ggttcgaccc gcctcgtcac tcacctgaga caactcctcc
     4021 cattgctcct gggacaatag ctccagatcc ttcgcgagaa gggcaaagta tgaaacctca
     4081 gcgagcgatc cgagcgcagt atcgatgaat cggcggaatt ctcgggatcc acgctttgcg
     4141 gctccctcga ctaggttggc ggccgcgctc gaggccgcgc ggcgcatctg cgacgtcatc
     4201 ccatattctt ctgtctttgg gaacgtcctg gtgatccgat aggtgaccaa agcgagacgg
     4261 tggcaatggt gccatgcgat cagccgctca tgaggtttca tgcggtaaga tgacaggcca
     4321 ccaaccaaat cgcgtcacca gaaaaatgca ggcatgaacc ttttcgcgcg cttccctagc
     4381 cgtctagccg tctagccgtc tagttaggcc gcttgatgcg c
//
LOCUS       DASUBP010000260        23554 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_676919, whole genome shotgun sequence.
ACCESSION   DASUBP010000260 DASUBP010000000
VERSION     DASUBP010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 23554)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23554)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23554
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_676919"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..1484
                     /locus_tag="VFU75_09745"
     CDS             78..1484
                     /locus_tag="VFU75_09745"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEU5467163.1"
                     /translation="MSTRPACLILATVVLGSCHSHTPAPASAQTKQGQQLKPAARTEG
                     RSAAVLVRLSVKGGTPRLYRLPSLAELPGTLRGRLPAVDRVIGTDDADGLLFLHSAKG
                     DAIAYDLQSGRADTVATGVTMAALGPDGTLFTIDAKKRVVSYSHRSKTAWPQPLAATP
                     AAIFGGADQRLIAVTSEKKPRLVAETLDQPAVMRPYPGSGVVEAARFGDEVASATDSG
                     LVLIEPVGRRAAAFVPLTDHPTSVAFSPAGHRLYVSRKTGFGLAVVDRFTRDEMDGVA
                     LPGMARALRLDPLGRWLLAQAGVGDSVWIVDLPTKTLTATVAGAWGDDLPVVSLDGTV
                     LLRQGADVVALRPDSGQPVGKVSGAAGDLWVATGWIPSGSSRSAALADAPGPAAAAGD
                     TAAGALYVQVSISQNKEWSDGMAQQLLRAGLRATVLPPTSTDDGYRVVLGPYATREQA
                     EDIGRKLGRPYWIYQPPQ"
     gene            1481..2668
                     /locus_tag="VFU75_09750"
     CDS             1481..2668
                     /locus_tag="VFU75_09750"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HEU5467164.1"
                     /translation="MTTRHVTRGDAQLSVPPLLTGKTLIALVPLGSDLEWAGRAAWDI
                     ARGCAVNRRVALVDLRLADPTLHRVPQLVAGEGVVDALEYDRSWDLVAREVDRVFFIG
                     TGGDAPDADAVLAHPRWRALHAGFRSENALLFVFCSASTVDLLGSAPDGVVLLTPVGE
                     AAAGFQPPARDAEILAVVSGPEAAPRLTRPAFASRSGTVPRRRTWGPLAAGLGLILLG
                     AVGWTILGRGAERVPRAAPIIAAPRADSLPWTVQLAAYGAPEKAVAAADRLTEEGVGT
                     FVTPLSLDASGALWYRVLVGAYATRQGAAAARADLWRRGLAPTGQGELLHAPFSLQLN
                     AAGTPPLDSLRHLGVPPVRWGGSGLQLVGAFETPEQASAAAAALGGAGVTATLITRMG
                     TTP"
     gene            2665..6132
                     /locus_tag="VFU75_09755"
     CDS             2665..6132
                     /locus_tag="VFU75_09755"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEU5467165.1"
                     /translation="MRLTRLELSGFKSFAGTVELPFDLGVTAIVGPNGCGKSNISDAV
                     RWVLGEQSPRLLRGGKMEDVIFQGSTGRRPVNVVEVSLVFDNSDGVLPIAYQEVVVTR
                     RLSRSGQSDYLLNRSPVRLRDIQDLLRGTGLGADAAVVMEAKMIDALLSEKAEERRGL
                     FEEAAGIGLYRDRKRSTERRLEETAADLARLEDLISEVQTQVRSLARQRGKAERYVKM
                     IDERFGVAMTLVRREMEDLDLALGALGVRTRELDENMPAARQRLADAEREREAQVQGR
                     HTAEAARTEVERRLADARIAVGRLEGDLKLAAERLANAAERRGRAGEERAQDEQRAAQ
                     AERERDAAQAECTSAETDLRAVRAELEGRTALEAEVRERLVRARDAVRLLDEELRKHV
                     ERTHAADAERTLLERERGELREQAARASAERGTRDQAAAAAQAAAQAQTQRAAAQAQA
                     AVRAVEGLQQARQRLASLQEAEVAARTTRRRAEEALAQLTARREALTELDRERVGLAP
                     AAQALLKVKSQFGDAVVGPLSDFVRTSRRDAELAERLLGEWLHGVLVRDESAIAEIQR
                     WHESAQPGPLVLLPCYPGPRQGADGHPLKDDLRVDGPAVAWVRALLAGHEVMSGARAL
                     KRANGAVFLDSAGSAGPLQRRAELEQLEQEITEATKASARAESDLDAATRDVAAAEAA
                     VTEAGETAERERHLELETGAQKGEAERAAAHARRDAGEAAAQADRLTRRLEDVDARLG
                     TLTADLQALEIERMRAEERLSAERATLAGLEAEQEAAREQRVHWQVDAAQVEARLAAA
                     RERSGRAAQDAEALRQHAVVLAAEIESIDRDTATLTTERSGWEDSLQERRIALAELEG
                     ATEGAGSAVSEAEAGVTAAENALNEARIALDALGDEAHRLEVERTEVDGRKRGLAERV
                     EVEWKKPLDQLLAEAPEVEGDLEWLRSENERLKNAIEQVGPVNALAVEEHAEEVKRLE
                     FLMTQRDDLVAARSSLQQAAREIDQTAKTLFLESFGKVRENFRSVFQTLFGGGECDVR
                     LANEDEPLDSEIEIHAAPRGKRTQRIHLLSSGERTLVAVSLLFSIFLTRPSPFCLLDE
                     VDAPLDDANVGRYVRLLSEFKTSTQFIVITHNPRTMQAADAVYGVTMQEPGVSTIVGV
                     RLGQLERTA"
     gene            6174..8432
                     /locus_tag="VFU75_09760"
     CDS             6174..8432
                     /locus_tag="VFU75_09760"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEU5467166.1"
                     /translation="MLASGTAYAQRGAVGSVPAVSAPLARAWSRDSTFTVWLFVRPGI
                     ALSQAATRATDAGAQIRRTSRWLHAVSVTASTSALRVLSQDPSFQRIQPLGRWRARPG
                     PDPTAPPRTAIVLAPDTCGAAGDPTYGPSEMPYRQLNLRGLADHGVDGTGVRIAILDA
                     GFNTLNPAFAGVTVTAQYDFVFGDSVVRNQPNDSLLLDPQFHGTAVWSLFAGSVSGRL
                     VGIARGASYLLAKTEDVRSETRVEEDNYVAALEWADSIGVDIVSSSLGYLAFDNGFSY
                     TPSQLNGDVAVTSVAAESASAHGILVVTAAGNGGPGFRTLVTPGDAKPVVTAGAEDSL
                     GTIASFSSRGPAADGRLKPDLTAPGVAVCTVTGTGTLGRLNGTSFATPILAASAALLK
                     EAQPTLGPLALRAALRRYAANAARPDSTRGWGRPDVTASAIFPNGVTPLSPLPPALTS
                     VTPQFSWTVGTTPGFAGPVTYRLRIARDSLLANPILDSLMGSTTQYLPSRAFKPGAPI
                     YWRVDGTAMGVTATTGVVGPITVPAWATLVTFNDSAGVTTDSVQPTFSWTAPAIASPP
                     GPFQFDLQIFRPGQPIPVYGAAGISATSFRVPVALERNAPYFWRLTVHAGPDTTQVSS
                     VGVFIILDASAPQVTLLYQNFPNPFPAVGRDSTCIWFDLANPGQTDLQILDLRGDVIR
                     RIIPRQALGGVLPPGRYGRGPLGGSTCDPEITWDGRADDGRFVPAGVYILRLRSGTTQ
                     IYKRLVFMGPRH"
     gene            8499..9539
                     /gene="aroF"
                     /locus_tag="VFU75_09765"
     CDS             8499..9539
                     /gene="aroF"
                     /locus_tag="VFU75_09765"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEU5467167.1"
                     /translation="MLIVMRHGATDAEVQRVVAVIQEMGYQAQAMPGAQRTAVGLIGN
                     DGRVDASRLEGLPGVTEVIHVSKPYKQVSREWKSESTVIQFPNGVTVGGTDVVAIAGP
                     CSVESERQIIDTARQVKEAGATILRGGAFKPRSSPYSFQGLGRPALRFLEKARAETGL
                     LVCTEAMDIEGVGWVSEMADIVQIGARNMQNYSLLKAAGRCGKPVLLKRGMAATITEL
                     LLSAEYLLAEGNGEVMLCERGVRGFDPITRNLFDLTAIPVVHKLSHLPIIADPSHGTG
                     LRDQVLPMARAAVAAGADGIIVEVHPAPDQALSDGAQSLFPKQFAQLMKEVRIIAEVT
                     GRRMAEPASVGA"
     gene            9536..10225
                     /locus_tag="VFU75_09770"
     CDS             9536..10225
                     /locus_tag="VFU75_09770"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HEU5467168.1"
                     /translation="MSREPGGPKGLPDGRGSRLLLLLLLSLPGASLAAQDPFAVLARA
                     RAAYDTIHTLRADFVQIVDNPMVGAPDTTRGILYQQPPSRFTMNFTHPKGDRVLSDGT
                     YLWVYTPSTTPGQVIRTRLPSNGGTGPNLIGQFVEHSEERYHARFLRTDSTAAGVTDV
                     IHLEPIKADQPYAAADLAIARSGLVTRLDIMETSGQKRVLIFSKPQLNAGVSGRTFRF
                     TPAAGVRVVDQ"
     gene            complement(10188..11066)
                     /locus_tag="VFU75_09775"
     CDS             complement(10188..11066)
                     /locus_tag="VFU75_09775"
                     /inference="COORDINATES: protein motif:HMM:NF022188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HEU5467169.1"
                     /translation="MGVVGIIGAGRVGAGLALALTRTGREIRLISRRPVRVPAPLSLK
                     VAPDGPDAEWVSGLEVLFLAVPDDRIHPVAQQLWGTKSIGPGQTVLHCSGVLGQEALS
                     ALVPTRAALGSLHPLQTISDPEVAPERFKGAWAAVEGSPAAMEAAEDIARSLGMKPFR
                     ITAKQKILYHAGAVFASNYLVVVEAIAQRLLRHAGLSDEEAWAALSPLVRGTITNLLA
                     ADPRSALTGPVVRGDTDTILRHLGALTIDDARLYQMLGRAALELARKGGMDDKTAQKV
                     ADALATDLPRVLQPPE"
     gene            complement(11074..12690)
                     /locus_tag="VFU75_09780"
     CDS             complement(11074..12690)
                     /locus_tag="VFU75_09780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU5467170.1"
                     /translation="MKGKNIAIIAVVTGAALVTGGWLLQRDTVPTGSVYEQARLFDDV
                     LAHVADYYVDSIDEGKLYGMAIDGLVDQLHDPYSVYLKPDDFKELTEQISGDYGGLGF
                     QFEVRDNAVAVVAPLPETPAERAGLLGGDMITAVDGRATAGWKQDDVFKKMRGPAGTV
                     VTLTVKRPGVDQPMTFKITRAKIHVRSVGLAMMLDDHVGYIAMSPVSDSSAHEMEVAV
                     DSLTKAGMRALIYDLRGNPGGSLQQGVGVTDLFLNPGQEVVATKGRAPHSNAVYRDDE
                     PQRWPNLPIVVLVNGFTASAAEIISGALQDHDRALVVGTPTWGKGLVQQVWQLTPETA
                     LKLTTARWYTPSGRTIQRTMKSEEEQVALVLAAEHGQDTSKIDTTHVFKTDAGRVEYG
                     GGGIRPDLFVAVDTFTTGEQAFNKSLGAKAPVFADVLSGYAVDAKVHHLVSSPDFQVT
                     DAMVTEVLDRLRARGVTFTPAILQGGADLLKQEIGYEVARYSFGRIGEFHRRISDDQQ
                     VVRALDLARKAKTPQELLSLAAKPQPHPGR"
     gene            complement(12687..13310)
                     /gene="tmk"
                     /locus_tag="VFU75_09785"
     CDS             complement(12687..13310)
                     /gene="tmk"
                     /locus_tag="VFU75_09785"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEU5467171.1"
                     /translation="MFIVVEGPEGAGKSTLVRGINARFLAEGRQVLMVREPGGTPVAE
                     AARKIVLKSRHDLSPASELFLYLAARADLVEKQIRPALAAGQVVLADRFDLSTRAYQV
                     AGRGLPADAVQAALQVATGGLTPDLTLVLDVPVEVGRERQRKARKEQDRIERESDAFH
                     HRVLEAYRKAAGPGVVHLDATQAKSAVLEAAWKEIGAASALSLRGIP"
     gene            complement(13312..13665)
                     /locus_tag="VFU75_09790"
     CDS             complement(13312..13665)
                     /locus_tag="VFU75_09790"
                     /inference="COORDINATES: protein motif:HMM:NF017902.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEU5467172.1"
                     /translation="MIDDKAVELGRLLGQTEEYKALKRANDRMREEQDVLKQLAEVER
                     LAVEIERGAQAGIEPAKEQVEAYDRALHAVQGHPVYQQVVAAQANFEKLMAKVNERIF
                     EGIKKGSASPIITLG"
     gene            complement(13662..14540)
                     /gene="prmC"
                     /locus_tag="VFU75_09795"
     CDS             complement(13662..14540)
                     /gene="prmC"
                     /locus_tag="VFU75_09795"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEU5467173.1"
                     /translation="MPEDPSGSVGEVLSRATERLQRAGVLNPKRESTALWAAMVGRTP
                     GDVWLDREGAAEPGAVDRFWTAVGRRAGGAPFPYAVGRVGFRNLDLVVDARALIPRPE
                     TEGLVGLVLEWAGNRHGGQGLAADVGTGTGCIALALAQEGDFSGVVATERSPQAAALA
                     RENVARVAPKVPVEVREGDLLGPLAGSRYRVIVSNPPYLTEQEYDALEPSVKSYEPRE
                     ALVSGVDGLNATRALLAGAAALLEPGGLLALEIDERRGPDVRALAHEHGWRRIGIHDD
                     LFGRPRFALAFPGEGL"
     gene            complement(14533..15591)
                     /gene="prfA"
                     /locus_tag="VFU75_09800"
     CDS             complement(14533..15591)
                     /gene="prfA"
                     /locus_tag="VFU75_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEU5467174.1"
                     /translation="MEARLRQALARAEEVARLLADPSTARNPAALKQLGREHARLDVI
                     RQTNQRLERLTEELAQAREVLQDKDPELAQLARADVERLRPEVASTEAELAELLVPAD
                     PLDDRDAIVEIRAGTGGDEAALFAADLYRMYTRFCERKRWKIEVTELSDATLGGIKEV
                     IFAVRGPKVYGTLRYESGVHRVQRVPETEAQGRIHTSAATVAVLPEAEEVDVTIEEKD
                     LRIDVFRSSGPGGQSVNTTDSAVRVTHLPTGLVVQCQDQKSQLQNKIKALEILRSRLL
                     DRMIAEQEAARARDRRAMVGTGDRSAKIRTYNFPQNRLTDHRIHFTTHNLTDVMNGDL
                     DAVITALRSEGEKERMSA"
     gene            complement(15595..15807)
                     /gene="rpmE"
                     /locus_tag="VFU75_09805"
     CDS             complement(15595..15807)
                     /gene="rpmE"
                     /locus_tag="VFU75_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010242421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HEU5467175.1"
                     /translation="MKPGIHPNYQRAVVTCACGNTFITRSTTPAINVEICGKCHPYFT
                     GKQKLIDTAGRVERFRQKYGTGKAAS"
     gene            15958..16629
                     /locus_tag="VFU75_09810"
     CDS             15958..16629
                     /locus_tag="VFU75_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEU5467176.1"
                     /translation="MLASEVLRKVPLFSELSQADFQRVLDVARERTHPKNSVILFEDD
                     PGDALYVVCGGQVKVVLIGEDGREVILSVLGEGEFFGEMALIDDEPRSAHVIAMEDST
                     LVVLRREDFAPILSTTPAIAIALLRELSRRLRRVDEKVGSLVLLDVNGRVARLLLEMA
                     DENGGERITRRLTHHTIAQMIGSSRETVSRTMRELVDKGLIEVGRKEILVKNRPALAE
                     AAGRG"
     gene            16707..17582
                     /locus_tag="VFU75_09815"
     CDS             16707..17582
                     /locus_tag="VFU75_09815"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEU5467177.1"
                     /translation="MSDRKYSVTFWGTRGSIPTPGAHTARYGGNTPCVAVEGAGGKLV
                     ILDAGTGIRALGGKLVERQNGAVTAEILLSHAHWDHIQGLPYFKPFFSPGNQVRIWGS
                     QQGEVSLEDILRQQMHPSVFPVPLDALSARLTVEHVGSGEFPVGEFKVRSTRLRHPGT
                     TLGYRLTPAEGGASLAYVTDNELGPGGKYETGPGWRQELVGFLNGVDVLIHDAMYTPD
                     ELKTHAGWGHSSYTEAVALAKEAGVQRLVLFHHEPEHSDGAMDDVLAAARKEAKKLGG
                     PGEVLAAREGLQLTL"
     gene            17618..18325
                     /locus_tag="VFU75_09820"
     CDS             17618..18325
                     /locus_tag="VFU75_09820"
                     /inference="COORDINATES: protein motif:HMM:NF015724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CsgG/HfaB family protein"
                     /protein_id="HEU5467178.1"
                     /translation="MRSRSMLALLALVGFAGPAVAQQATTTDTRPGVAVFAFENGGSI
                     GMKDPADMPALQRGFAGMMINELKANDAWRVVEREEIQKLTDEQNLDASGRVDPQTAA
                     KIGKIVGAKYAVTGTFIANGTNWRLDIRLINVETTEIMKTESQTASQDKLFSIVTDVA
                     QRLMKDASLPPLPKDVATQRMSKPVPTEALTFYSRALLYHDRGNKEKAVEMYKAALNV
                     FPDYVPAQEGLKKEQQG"
     gene            18333..19100
                     /locus_tag="VFU75_09825"
     CDS             18333..19100
                     /locus_tag="VFU75_09825"
                     /inference="COORDINATES: protein motif:HMM:NF014460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5467179.1"
                     /translation="MNFIGRLGNIVARRLSQLGALARFGFGALRALVTLPVAGRAVAF
                     RVTMNQIRFTAVHAVALIVLLSSILSFLVISQAARELGSIGKTEWLGTLMVVVIVREL
                     GPLVTALAVAGRSGTAIAAELATNQVMGEVRALEGMGIDPRQYLVVPRFVGGLVSVFI
                     LMILFDVVAVAGGLGAATFNGMAPGRYMDVVLQALTLRDVWLTVAKALAFGIVVGVVP
                     SFYGLAVRRGPTEIPIAASRATVSSIVGIFLGAALFG"
     gene            19104..19796
                     /locus_tag="VFU75_09830"
     CDS             19104..19796
                     /locus_tag="VFU75_09830"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5467180.1"
                     /translation="MTGRPSVFEGAPLVFDQVLISPRAATPFSLTVARGETVVIIAEE
                     DVPLGPMALALDPPRSGRVLVLGTDLTDLPERERLAFRRRVGYLPAGDGLLQNLSLRD
                     NVALPLRFGSTTGSKEIEGRVEVMLAAARIGRIGGLRPAQANEEERRRTALVRALAFD
                     PSLLILEQPFDGLSSRVAGELLTLARGGEEVQGGRRSVLITCQELPPLLHNRVERIYR
                     LDRQGVLELEPA"
     gene            19812..20726
                     /locus_tag="VFU75_09835"
     CDS             19812..20726
                     /locus_tag="VFU75_09835"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HEU5467181.1"
                     /translation="METNRQDFIVGLLIVVAVGLVIGALLATSGWGQRRYALYMRVAN
                     AEGISADTRVLVEGLEIGRVASIVPRVDPTSRRVSFIARLSLAQEFENGSKLLLPLGT
                     KASIASVSQISSASEIVLILPDSTHGVARTLGAGDTINSDRPSSALDSLKVVVTSLSS
                     DVRDVLLRARDALVGVQRTLASANSTIQTMTPSLSAAVTSVSSSMARIDSITRRIPPG
                     LADSVTATLASSNRLLVHLDSVVQSAQVMASENRGDLRATATNLAELSRQLNHFADAV
                     SRRPYRFLTGVTPLPPDKADPPKDSTKP"
     gene            20723..22678
                     /locus_tag="VFU75_09840"
     CDS             20723..22678
                     /locus_tag="VFU75_09840"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HEU5467182.1"
                     /translation="MNARYILLHPTGWSPTMVVDALASAQIEARAISGPVELEVDERP
                     TVFLLDAGSRSAFPLDAQRAFVDAGGAIIALGREGELDVPEPFATELLAAFVRHPVGP
                     RQLLIAVRSGLREAAARREAARAMAQAEQRSQEIGEFTRIGAALGTERDLKTLLELIL
                     TQARRITQSDAGSLYLVEAADSGAPRLRFSLAQTFSKPEAPFVEFTIPMDRSSLAGYT
                     AVTGDPLVIDDAYYLPPDVEYSINRSFDERYGYRSKSMLVIPMKDHRDQRIGVLQLIN
                     RKRNSDAVLGTAADVEREVVPYSRRSVELVTALAAQAAVAIENSRLYQEIERLFEGFV
                     RASVQAIEQRDPTTFGHSGRVADYTVSLASVVDRAGAGPYKAVRFTREQVREIRYAGL
                     LHDFGKVGVREQVLVKAKKLYPPDLDLIRQRHAFVRRSLERDFWQRRAEFLEEHGKKG
                     YPAFQEKLDEEHRKALADLDRFLETVLQSNEPSLLPEQSKHDLSQFARRTYADLAGAP
                     QPLLTPDEVRYLTIPKGSLDEAERLEIESHVTHTFNFLKEIPWTRELKRIPDIAYGHH
                     EKLDGRGYPRKIQGEAIPIQTRMMTISDIFDALTAQDRPYKRAVPAERALDIMKQEVT
                     QGQLDGELYRLFVEGRVFEPAAARGDG"
     gene            22675..23511
                     /locus_tag="VFU75_09845"
     CDS             22675..23511
                     /locus_tag="VFU75_09845"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEU5467183.1"
                     /translation="MNATLFAALVFGGSFLAGLLGSLTGLGGGMVIVPLLAIGFGVNL
                     QYAIGASLISVIATSSGAAAAYVREGYTNIRIGMFLEIATTVGALGGAFIAGMISTSA
                     IAIVFAGVLLYSAWLSARPRVEHVTQDLSDPLARSLRLHGTYPTPDGLQPYGVQRVAT
                     GFSLMFVAGILSGLLGIGSGAVKVLAMDHAMRLPFKVSTTTSNFMIGVTAAASAGVYL
                     RRGYIDPVLSFPVMLGVLAGALLGARILTKTHPKHLRLVFSVVIVALAIEMVYRAIQG
                     TI"
BASE COUNT         3594 a         8245 c         7903 g         3812 t
ORIGIN      
        1 ggcccgacgt tctaactccg ttcggctgaa ccacttgccc cggcctcgcc gacttcctat
       61 tttgtcggcg aggccatttg tctacccgcc ccgcgtgtct catcctggcg accgtggtgt
      121 tggggagttg ccactcccac acaccggcac ccgcatcggc gcaaaccaag cagggacaac
      181 agcttaagcc ggcagcacgt accgagggcc ggtccgccgc ggtcctggtt cgcctgtccg
      241 tcaaaggcgg caccccaagg ctctaccgcc tcccctctct cgccgagctt cccggaacgc
      301 tgcggggacg cttgccggcc gtcgatcgcg tgatcggcac cgacgacgcc gacggcctgc
      361 tgtttctcca ctcggccaag ggcgacgcca tcgcctacga cctccaaagc ggccgcgccg
      421 acaccgtcgc gaccggcgtg accatggccg ccctcggccc cgacggcacg ctcttcacca
      481 tcgacgcgaa gaagcgggtc gtgagctact cgcatcgctc gaaaacggcg tggccacaac
      541 cgctcgccgc gacccccgcg gcgattttcg gtggcgccga tcagcggctc atcgccgtca
      601 cctcggagaa gaaaccgagg ctcgtcgccg agacgctcga ccagcccgcg gtgatgcgcc
      661 cctacccggg atccggtgtc gtcgaagccg cgcgtttcgg tgacgaagtc gcgtcggcga
      721 cggattcggg actcgtactc atcgaaccgg tgggcaggcg cgccgcggcg ttcgttccgc
      781 tcaccgacca ccccaccagc gtcgcgttct cccctgccgg acatcgtctc tacgtgagcc
      841 ggaagaccgg attcgggctc gcggtggtgg accggttcac gcgtgacgag atggatggcg
      901 tcgcactgcc gggcatggcg cgcgccctgc ggctcgatcc gctcgggcgc tggctcctcg
      961 cgcaagcggg agtcggtgac tcggtgtgga tcgtggatct gccgacgaag acgctcacgg
     1021 cgacggtcgc gggtgcgtgg ggcgacgatt tgccggtggt ctccctggac ggaaccgtcc
     1081 tcctgcggca aggggcggat gtcgtcgcgc ttcggcccga ctcgggccaa ccggtcggca
     1141 aggtgagtgg tgccgccgga gatctctggg tcgcgaccgg atggattccg agcggctcgt
     1201 cccgctccgc ggcgctcgcg gacgcgccgg gacccgccgc ggccgcgggt gacaccgccg
     1261 caggtgcact ctacgtccag gtctccattt cacagaacaa ggaatggtcc gacggcatgg
     1321 cgcagcagct gctgcgcgcc ggcttgcggg cgacggtgct gcccccgacg tccacggatg
     1381 acggctatcg cgtcgtgctg ggaccctatg ccacccgcga gcaggccgag gacatcggcc
     1441 ggaagctggg acgcccgtac tggatctacc agccgccaca gtgaccacgc gccacgtcac
     1501 gcgcggcgac gcgcagctct cggtcccgcc tctgctcacg gggaagacct tgatcgcgct
     1561 cgtgcctctg ggctcggacc tcgagtgggc gggacgcgcc gcctgggaca tcgcgcgggg
     1621 gtgcgccgtg aaccggcggg tcgccctggt cgacctgcgc ctcgccgatc ccaccctgca
     1681 tcgcgtcccc cagctcgttg ccggtgaagg agtcgtggac gcgctggagt atgaccggtc
     1741 ctgggatctg gtggcgcgcg aggtcgaccg ggtgttcttc atcggaacgg gtggtgacgc
     1801 gcccgatgcg gatgccgtcc tcgcgcatcc gcgctggcgc gccctgcatg ccgggttccg
     1861 gtccgaaaac gccctgctct tcgtcttctg ctccgcgtcc accgtcgatc tgctgggttc
     1921 cgcgcccgat ggcgtggtat tgttgacgcc ggtcggcgaa gcggccgccg gatttcagcc
     1981 ccccgcccgc gatgccgaaa tcctcgcggt ggtgagcgga cccgaagcgg cgccccgcct
     2041 cacgcgaccg gcgttcgcgt cccgctccgg gaccgtcccc cggcgccgca cctggggtcc
     2101 cctggcggcc ggcctcgggc tcatcctgct cggcgcggtc ggctggacca tcctggggcg
     2161 tggcgccgag cgcgtccccc gtgccgcgcc catcatcgcc gcgcctcggg ccgattccct
     2221 tccctggacc gtgcagctgg cggcctatgg tgcccccgag aaggcggtgg cggcggcaga
     2281 ccgtttgacc gaagagggcg tggggacctt cgtcaccccg ctctcgctcg atgcgtcggg
     2341 cgcgttgtgg tatcgcgtcc tcgtgggcgc ctacgcgacg cgccagggag ccgccgccgc
     2401 ccgcgccgac ctctggagac ggggcctcgc gcccaccggt caaggggagc tcctgcacgc
     2461 gccgttttcg cttcagctca acgccgctgg gacgccgccg ctcgattccc tgcgccacct
     2521 gggggtaccc ccggtgcgct ggggcggctc cggcctgcag ctcgtcggtg cttttgaaac
     2581 acccgaacaa gccagtgccg cggccgccgc tctcggcggt gccggagtca cggccacact
     2641 cattacccgt atggggacca cgccatgaga ctgacgcggt tggagttgtc cgggttcaag
     2701 tcgttcgccg gcacggtgga actgcccttc gacctgggcg tcacggccat cgtcggcccc
     2761 aacggatgcg gcaaatccaa catctcggac gccgtccgct gggtcctcgg cgaacagtct
     2821 ccgcggctcc tccgcggcgg aaagatggaa gacgtgatct tccagggctc caccgggcgc
     2881 cgccccgtca acgtcgtcga agtctcgctg gtgttcgaca actccgacgg cgtgctcccc
     2941 atcgcctacc aggaagtggt cgtcacccgc cgcctctccc ggtccggtca gagcgactac
     3001 ctgctcaacc gctctccggt caggctgcgc gacattcagg acctgctgcg cggcacgggg
     3061 ctcggcgccg acgcggccgt agtgatggaa gcgaaaatga tcgacgccct gctctcggaa
     3121 aaagccgaag agcgccgcgg gctgttcgag gaagcggccg ggatcggcct ctaccgcgac
     3181 cggaagcgca gcacggagcg tcggctcgag gagacggcgg ccgacctggc gcggctcgaa
     3241 gacctgattt ccgaggtgca gacccaggtg cgttccctcg cccgtcagcg cggcaaggcg
     3301 gagcgctacg tgaagatgat cgacgagcgc ttcggggtgg ccatgacgct cgtccgccgt
     3361 gagatggaag acctcgacct cgccctcggc gcgctgggcg tccgcacgcg cgagctcgac
     3421 gagaacatgc ccgcggcccg ccagcgcctc gccgacgcgg agcgggagcg cgaggcgcag
     3481 gtacagggcc gccataccgc ggaagccgcg cggaccgaag tcgagcgccg gctggccgat
     3541 gcccgaatcg cggtgggacg gctcgagggt gacctgaagc tcgccgccga gcgcctcgcg
     3601 aacgcggcgg aacgccgtgg ccgcgcgggt gaggagcgtg cccaggatga gcagcgcgcc
     3661 gcgcaggccg agcgggagcg cgacgccgca caggcggagt gcacctccgc cgagaccgac
     3721 ctgagggcgg tgcgcgccga gctggaaggc cgcaccgcgc tggaggcgga agtccgtgag
     3781 cgcctcgtgc gtgcacggga tgccgtccgg ctcctggacg aagagctgcg taagcacgtc
     3841 gagcgcactc acgccgcgga cgccgagcgc acactgctcg agcgggaacg cggcgagttg
     3901 cgcgagcagg cggcacgggc atccgcggag cgcggcaccc gtgaccaggc ggcggcggcc
     3961 gcgcaggcgg cggcgcaggc gcagactcag cgcgccgcgg cgcaggcgca ggcggcggta
     4021 cgcgcggtcg aagggctgca acaggcgcgg cagcggttgg cgagcctcca ggaggcggag
     4081 gtcgcagccc gcaccacccg tcgccgcgcc gaagaggcgc tggcccagct caccgcacgt
     4141 cgcgaggcgc tcaccgagct cgatcgcgag cgcgtcggcc tggcccccgc cgcgcaggcc
     4201 ctgctcaagg tgaaatcgca gttcggggat gcggtcgtcg gccctctgtc ggatttcgtg
     4261 cgcaccagcc gccgtgatgc ggagctggcc gagcgcctgt tgggcgagtg gctgcatggc
     4321 gtgctggtca gggacgagtc ggccatcgcg gaaattcagc gctggcatga gagcgcccag
     4381 cctggtcccc tcgtacttct cccctgctat cccgggccgc gtcagggcgc cgacgggcat
     4441 ccgctgaagg atgacctccg cgtcgacggc ccggcggtcg cctgggtgcg ggcgttactc
     4501 gccggtcacg aagtgatgtc gggcgcccgt gccttgaagc gcgcgaacgg cgccgtcttc
     4561 ctcgactccg ccgggtccgc gggcccgctg cagcggcgcg cggagctgga gcagctcgag
     4621 caggaaatca ccgaagccac caaggcgagt gcccgggccg aatccgatct cgacgccgcg
     4681 acgcgcgacg tcgcggccgc cgaggcggcg gtgacggagg cgggtgaaac ggcggagcgg
     4741 gaacggcacc tcgagctcga aacgggtgcg cagaaaggcg aagccgaacg tgccgcggcg
     4801 cacgcgcgcc gggatgccgg tgaggccgcg gcccaggccg accggctgac ccgccgactc
     4861 gaagacgtgg atgcccgcct cggaaccctg acggcggacc tgcaggctct ggaaatcgag
     4921 cggatgcgcg ccgaggagcg gctctcggcc gaacgcgcga cgctcgcggg gctcgaagcc
     4981 gagcaggaag cggcgcgcga acagcgggtg cactggcaag tcgacgccgc gcaagtcgaa
     5041 gcgaggctcg cggcggcccg ggaacgcagc ggacgggcgg cgcaggatgc cgaggcgctc
     5101 cgccagcacg ccgtggtcct ggccgccgaa atcgagtcca tcgatcgcga cacggcgacc
     5161 ttgacgacgg aacggagcgg ctgggaggac agtctccagg agcgccggat cgcactcgcg
     5221 gaactcgaag gcgccaccga aggcgcgggc tccgcggtgt cggaggcaga ggcgggcgtg
     5281 acggccgccg agaacgcctt gaacgaggcg cggatcgcgc tcgacgccct gggggatgaa
     5341 gcgcaccggc tcgaggtcga acggaccgag gtggatggcc gcaaacgcgg tctggcggag
     5401 cgcgtggaag tggaatggaa gaagccgctc gaccagctcc tggccgaggc tcccgaggtc
     5461 gaaggtgatc tcgagtggct ccgctcggag aacgagcgac tgaagaacgc catcgagcag
     5521 gtaggccccg tcaacgccct cgcggtcgag gagcacgccg aggaggtcaa gcggctcgag
     5581 ttcctgatga cccagcggga tgacctggtg gccgcccggt cgtcgctcca gcaggcggcc
     5641 cgggaaatcg accagaccgc gaagaccctg ttcctcgagt cgttcggcaa ggtccgggag
     5701 aatttccgga gcgtcttcca gacgctcttc ggcggcggag agtgcgacgt gcggctggcc
     5761 aatgaggacg agcccctcga ctcggagatc gagatccacg cggcgccgcg gggcaagcgg
     5821 acccagcgga tccacctcct ctcgtcgggc gagagaaccc tggtggccgt gtccctgctc
     5881 ttctcgatct tcctcacccg gcccagcccc ttctgcctcc tggacgaggt ggacgcgccc
     5941 ctggatgacg ccaacgtcgg gcgctacgtc cggctcctgt cggagttcaa gacctcgacg
     6001 cagttcatcg tcatcacgca caatccccgc accatgcagg ccgccgacgc cgtctacggc
     6061 gtgaccatgc aggaaccggg cgtctccacc atcgtgggcg tccggctggg acagctcgag
     6121 cgcaccgcct gagctcccgc gccgccgtca tccgggggct cctcctcgga accgtgctgg
     6181 cgagcggcac ggcgtacgcc caacgtgggg ccgtcgggag cgtcccggcg gtttcggccc
     6241 cgctggctcg tgcctggtct cgagactcca cgttcaccgt ctggctgttc gttcgacccg
     6301 ggatcgcgct gagccaggcg gccacgcggg ccaccgacgc gggcgcgcag attcgccgta
     6361 ccagccggtg gctccacgcg gtcagtgtca ccgcttcgac gagcgcgctg cgcgtcctct
     6421 cgcaagaccc atcgtttcag cggatccagc ctctgggacg ctggcgggcg cgcccgggac
     6481 ctgacccgac cgctccaccc cgcaccgcga tcgtgcttgc ccccgacacc tgcggcgccg
     6541 cgggtgaccc gacctatgga ccgtccgaga tgccctaccg tcagctcaac ctgcgggggc
     6601 tcgcggacca cggcgtcgac ggcaccggcg tgcgcatcgc cattctcgat gcgggattca
     6661 acacgctgaa tccggccttc gcgggtgtca ccgtcaccgc gcaatacgat tttgtgttcg
     6721 gggactcggt ggtccggaat cagcccaacg attccctgct cctcgacccg cagtttcacg
     6781 gcacggcggt ctggtcgctc ttcgccggga gcgtttccgg acgcctggtg gggatcgccc
     6841 gcggcgcgtc ctacctgctg gcgaagaccg aagacgtacg gtcggagacg cgggtcgagg
     6901 aggacaatta cgtcgcggcc ctcgaatggg ccgactcgat cggcgtcgac atcgtctcgt
     6961 cgagcctcgg gtacctcgcg ttcgacaatg gcttcagcta cacgccgagc cagctcaatg
     7021 gggacgtggc ggtgacgtcg gtggccgccg agagcgcttc cgcccacggc atcctggtcg
     7081 tcacggccgc cggcaacggc gggcctggct tccgcacgct cgtgaccccg ggagacgcga
     7141 agccggtcgt caccgcgggc gccgaagatt cgctggggac gatcgcctcg ttttcgagcc
     7201 gtggaccagc ggccgatggg cggctgaagc ccgaccttac ggccccgggc gtcgcagtgt
     7261 gcaccgtcac cggcaccgga accctggggc ggctgaacgg cacgtccttc gcgactccga
     7321 tcctcgccgc atccgcggcc ctgctgaagg aagcgcaacc cacgcttggc ccactcgcgc
     7381 tgcgggccgc tcttcgtcgc tacgcggcca acgcagcgcg gcccgactcc acccgcgggt
     7441 ggggacggcc cgacgtcacg gcatcggcca tttttccgaa tggcgtgacg cccctgtccc
     7501 ctctgccacc cgcgctcact tcagtgacgc cgcaattcag ctggaccgtc gggacgacgc
     7561 ccggcttcgc cggacccgtc acctaccgtc tccgcatcgc ccgggactca ctgctcgcga
     7621 acccgatcct ggattcgctg atggggagta ccacccagta tttgccgagc cgggcattca
     7681 aacccggggc tccgatctac tggcgcgtgg atgggacggc catgggcgtc accgcgacca
     7741 ccggcgtcgt cggcccgatc accgtccccg cctgggccac cctcgtcacc ttcaacgact
     7801 cggcgggagt caccaccgac tcggtccagc cgaccttctc ctggacggcg ccggccattg
     7861 cctcgccccc cgggcccttt cagttcgatc tccagatctt ccggcccggc cagcccatcc
     7921 cggtgtacgg tgcggccggc atcagcgcca catcgtttcg tgtccccgta gcactcgagc
     7981 ggaacgcccc gtacttctgg aggctgaccg tccatgccgg ccccgacacg acgcaggtct
     8041 cttcggtcgg ggtcttcatc atcctcgacg cctcggcccc gcaggtgacc ctgctctatc
     8101 agaacttccc caatccgttc cccgcggtcg gccgggactc gacatgcatc tggttcgact
     8161 tggcgaatcc cgggcagacc gacctccaaa tcctcgatct gcggggggac gtcatccgcc
     8221 ggatcatccc ccggcaagcg ctggggggtg tcctgccacc cggccgctac ggccgcgggc
     8281 ccctgggggg ctcgacgtgc gatccggaaa tcacgtggga tggccgcgct gacgacgggc
     8341 gcttcgtgcc cgccggggtg tacatcctga gactgcgcag tggaaccacc cagatctaca
     8401 agcggctggt attcatggga ccgcggcatt gacaagctct tgtcttgacg gccttcccgt
     8461 ttcctaggga gtattcggga ccagttccgg agcgactttt gctgatcgtc atgcgacatg
     8521 gggccacgga cgccgaagtc cagcgcgtgg tcgccgtgat tcaggagatg gggtaccagg
     8581 cgcaggccat gccgggggcg cagcgcaccg ccgtcggcct catcggcaac gacggccggg
     8641 tggacgcttc gcggctcgaa gggctgccgg gcgtcacgga agtcatccac gtctccaagc
     8701 catacaagca ggtcagtcgc gagtggaaga gtgagtcgac ggtgatccag tttcccaacg
     8761 gagtcaccgt gggtggtacc gacgtcgtgg ccatcgcggg cccctgctcc gtcgagtccg
     8821 agcgtcaaat catcgacacc gccaggcagg tgaaagaggc gggggccacg attctccgcg
     8881 gcggcgcgtt caagccccgc agctcaccgt attcgttcca gggcctcggc cggccggccc
     8941 tgcgcttcct cgagaaggca cgcgccgaga ccgggctcct cgtctgtacg gaagcgatgg
     9001 atatcgaggg ggtgggctgg gtgtcagaga tggccgacat cgtgcagatc ggcgcccgga
     9061 acatgcagaa ttactccctg ctcaaggccg cgggacggtg cggcaagccg gtgctcctga
     9121 agcggggcat ggccgccacc atcaccgagc tgctcctctc ggccgagtat ctcctcgccg
     9181 aggggaacgg cgaggtcatg ctctgcgaac ggggggtgcg ggggttcgat ccgatcaccc
     9241 gcaatctctt cgacctgacg gcgattcccg tggtgcacaa gctgtcacac ctgccgatca
     9301 tcgccgatcc gtcccatggc accggtctgc gcgaccaggt gctgcccatg gcgcgcgccg
     9361 cggtggccgc gggcgcggac ggcatcatcg tggaagtgca ccccgcgccc gatcaggcgc
     9421 tttcggacgg cgcgcagtcg ctctttccca agcaattcgc ccagctcatg aaggaagtcc
     9481 ggatcatcgc cgaggtcacg ggccgccgga tggcggagcc ggcgtcggtg ggggcgtgag
     9541 ccgggagccg ggaggcccta aaggactccc tgacggtcgc gggagccgtc tgctcctcct
     9601 gctcctgctg agcctcccgg gcgcctccct ggccgcccaa gaccccttcg cggtcctcgc
     9661 ccgcgcccgg gcggcatacg acaccattca cacgcttcgc gccgatttcg tccagatcgt
     9721 cgacaacccc atggtcggcg cgcctgacac cacgcgtggc atcctgtacc agcagcctcc
     9781 ctcccgcttc accatgaact tcacgcaccc gaagggcgat cgcgttctgt cggacgggac
     9841 gtatctctgg gtctacacac ccagcacgac ccccggtcag gtcatccgca cgcggctccc
     9901 ctcgaacggg ggcacgggtc ccaatctcat cggccagttc gtcgaacact cggaagagcg
     9961 gtaccatgcc cgcttcctcc gtacggactc gacggccgcg ggcgtgacgg acgtgatcca
    10021 cctcgagccc atcaaggccg atcagcccta tgcggcggcg gacctcgcca tcgcgcgctc
    10081 agggttggtg acgcggttgg acattatgga gacgtcgggg cagaaacgcg tgttgatttt
    10141 ctcgaagccc cagctgaacg ccggggtttc cggcaggact ttccgcttta ctccggcggc
    10201 tggagtacgc gtggtagatc agtagcgagg gcgtccgcca ccttctgcgc cgtcttgtcg
    10261 tccatcccgc ccttcctcgc cagctccagc gccgcgcgcc ccagcatctg gtagagccgc
    10321 gcatcgtcga tcgtgagcgc gcccaagtgc ctgagaatcg tgtccgtgtc accgcgcacc
    10381 acggggccgg tcagagcact gcgaggatcc gcggccagaa ggttggtgat cgtgcctcgc
    10441 accagcggcg acaacgccgc ccaggcctcc tcgtcggaca gtcccgcatg gcgcaggagc
    10501 cgctgggcga tcgcctccac caccaccaga tagttcgacg caaataccgc cccggcgtgg
    10561 tagagaatct tctgcttcgc cgtgatccgg aacggcttca tgcccaggga gcgcgcgatg
    10621 tcctccgcgg cctccatcgc cgccggactt ccctccaccg ccgcccaggc acccttgaag
    10681 cgctcgggag ccacctcggg atcgctgatc gtctgcaggg gatggagcga tccgagcgcc
    10741 gcgcgggtgg gaaccagtgc cgagagggct tcctggccga gcacgcccga gcagtgcagc
    10801 accgtctgtc cggggccgat gctcttggtc ccccagagct gctgggccac cggatgaatg
    10861 cggtcgtcgg gaacggcgag gaagagcacc tcgagccccg acacccattc cgcgtcggga
    10921 ccgtcggggg cgaccttgag ggacagtggt gcgggaacgc gaacgggccg gcggctgatc
    10981 agccggattt cacggccggt gcgggtgaga gccagtgcca gcccggctcc cacccgtccc
    11041 gcgccgatga tcccgaccac tcccacgagc gggttagcgg ccggggtgcg gctgcggctt
    11101 cgccgccagc gagagcagct cctgcggggt cttggccttc cgggccagat cgagtgcgcg
    11161 cacgacttgc tggtcgtccg agatccggcg atggaattcg ccgatccggc cgaagctgta
    11221 ccgcgcgacc tcgtacccga tttcctgctt gagcagatcc gcaccaccct ggagaatggc
    11281 cggtgtgaac gtcacgccgc gggcccggag ccggtcgagg acctccgtca ccattgcgtc
    11341 cgtcacctgg aagtcgggac tcgacaccag gtgatgaacc ttcgcgtcca cggcgtaccc
    11401 cgacaagaca tcggcgaaca ccggcgcctt ggcgcccagg gacttgttga aggcctgctc
    11461 acccgtggtg aaggtatcga ccgcgacgaa caggtcgggg cggatcccgc cgccgccgta
    11521 ttcgacgcgc ccggcgtccg tcttgaagac gtgcgtcgta tcgatcttgg aggtgtcctg
    11581 cccgtgctcc gcggcgagca ccagggccac ctgctcctct tcactcttca tggtccgctg
    11641 gatggtccgc ccactcggcg tgtaccagcg ggccgtcgtc agcttgagtg cggtctccgg
    11701 ggtcagctgc cagacctgtt gcaccaggcc cttgccccac gtgggcgtgc cgaccaccag
    11761 tgcacgatcg tgatcctgga gcgcgccgga gatgatctcg gcggcactcg cggtaaaccc
    11821 gttgaccagc accacgatcg gcaggttcgg ccagcgctgc ggctcgtcgt cccgatacac
    11881 ggcgttggag tggggggccc tgcccttggt cgccacgact tcctggcccg ggttgaggaa
    11941 cagatccgtc acgccgacgc cctgctgcag cgagccaccg gggttcccgc ggagatcgta
    12001 gatcagtgcc cgcatgcccg ctttggtcag cgagtccacg gcgacttcca tctcgtgtgc
    12061 cgaggagtcg ctgaccgggc tcatggcgat gtagccgacg tggtcgtcga gcatcatcgc
    12121 gaggcccacg gaacggacgt ggatcttggc gcgcgtgatc ttgaacgtca tcggctgatc
    12181 cacgccgggc cgcttcaccg tgagcgtgac gaccgtaccg gcggggccgc gcatcttctt
    12241 gaagacgtca tcctgcttcc agccggcggt ggctcgtccg tccaccgcgg tgatcatgtc
    12301 accgccgaga agaccggcac gttcggccgg tgtttccggg aggggcgcga cgaccgccac
    12361 ggcgttgtcc cggacctcga actggaatcc cagaccgccg tagtctcccg agatctgctc
    12421 ggtgagctct ttgaaatcgt cgggcttcag gtataccgaa tacggatcgt ggagctggtc
    12481 caccaggccg tcgatggcca tcccgtagag cttgccttca tcgatcgagt cgacgtagta
    12541 atcggccacg tgggccaaca cgtcgtcgaa gagccgggcc tgctcgtaga ctgacccggt
    12601 cggcaccgta tcccgctgca gcagccagcc gcccgtgacc agggcggcgc cggtgacaac
    12661 cgcgattatt gcgatgttct tgcccttcat gggattcctc gcagtgaaag cgcggatgcc
    12721 gcgccgattt ccttccacgc ggcctccaac acggcggact tcgcctgcgt agcgtcgaga
    12781 tgcacgacgc cgggaccggc cgccttccgg tacgcctcga gcacgcgatg gtgaaatgcg
    12841 tcgctctcgc gctcgatgcg gtcctgctcc ttgcgggcct tccgttgccg ctcccgcccc
    12901 acctcgaccg gcacgtcgag caccagcgtg aggtccggcg tgagtccgcc ggtcgcgacc
    12961 tggagggccg cctgtaccgc gtcggcgggc aatccgcgcc ccgccacctg ataggcccgg
    13021 gtcgacaggt cgaagcggtc ggccagcacc acctgaccgg cggcgagcgc gggccggatc
    13081 tgcttctcga ccagatccgc gcgcgccgcg aggtacagga agagctcgga ggccggactc
    13141 aagtcgtggc gggacttcag cacgatcttc cgcgccgcct ccgccaccgg cgtgccgccc
    13201 ggctcccgca ccatcagtac ctgccgccct tcggccagaa aacgagcgtt gatgccacgc
    13261 accagcgtgg acttccccgc cccctccggc ccctccacca cgatgaacat ctcagcccag
    13321 cgtgatgatc gggctggccg agcccttctt gatcccttcg aagatacgct cgttgacctt
    13381 ggccatgagc ttctcgaagt tcgcctgagc ggcgactact tgctggtaca ccggatgtcc
    13441 ctggacggcg tgcagcgcgc gatcataggc ctcgacctgc tccttcgcgg gttcgatgcc
    13501 ggcctgcgca ccgcgctcga tctcgaccgc cagccgttcg acctccgcga gctgtttcag
    13561 gacgtcctgc tcctcgcgca tccggtcgtt ggcgcgcttc agcgccttgt attcctcggt
    13621 ctggcccagc agccgtccga gctctaccgc cttatcatcg atcacagacc ctccccaggg
    13681 aacgccagcg cgaaccgcgg ccggccgaac agatcatcgt gaatgccgat gcggcgccat
    13741 ccatgctcat gtgccagggc gcggacatcg ggccctcgtc gctcatcgat ttccagcgcc
    13801 aggagccccc ccggctcgag cagagccgcc gctccggcca ggagcgcccg cgtcgcgttc
    13861 agtccatcca cgccgctcac cagcgcctcg cgtggctcgt aggacttcac ggacggttcc
    13921 agcgcgtcat actcctgctc cgtgagatag gggggattgg agacgatgac gcggtagcgg
    13981 gaccccgcca ggggacccag caagtctcct tcccgtacct ccacgggtac tttgggagct
    14041 acccgggcca cgttctcgcg cgccagcgcc gccgcctgcg gagagcgctc cgtggcgacc
    14101 acgccactga agtccccctc ctgcgccaac gcgagcgcga tgcatccggt tccggtgccg
    14161 acatccgccg cgaggccctg tccgccgtga cggtttcccg cccactcgag caccagcccc
    14221 accaggccct cggtctcggg ccgcggaatc agtgcccgcg cgtcgaccac caaatcgaga
    14281 ttcctgaatc ccacccgccc taccgcatac ggaaacgggg cacctcccgc ccgccgtccc
    14341 acggcggtcc agaaccggtc cacggcgccc ggttcggcgg ctccttcccg atccagccag
    14401 acgtcgcccg gtgtccgccc gaccatggcg gcccacagcg ccgtggactc ccgcttcggg
    14461 ttcagcacgc ccgcccgttg cagacgctcc gtcgccctgc tcagcacctc tccgaccgaa
    14521 ccggaggggt cctcaggcac tcatgcgctc cttctcgcct tccgaccgca gcgcggtgat
    14581 gaccgcatcc agatccccgt tcatgacgtc ggtgaggttg tgcgtcgtga agtggatccg
    14641 gtgatcggtc agccggttct gcgggaagtt gtaggtgcgg atcttcgcgg aacggtcccc
    14701 cgtacccacc atcgcccggc ggtcccgtgc ccgggcagcc tcctgctcgg cgatcatgcg
    14761 gtcgagcaag cgggagcgca ggatttcgag cgccttgatc ttgttctgga gctgggattt
    14821 ctggtcctgg cactgcacga ccagtccggt cggcaggtgg gtcacgcgca ccgcgctgtc
    14881 cgtggtgttc acgctctgcc cgccgggacc ggacgaccgg aagacgtcaa tgcgaagatc
    14941 cttctcttcg atggtgacgt cgacttcctc ggcctccggc aacacggcga cggtggcggc
    15001 ggaggtgtga atgcgtccct gcgcttcggt ctcgggcacg cgctggaccc ggtgcacccc
    15061 ggactcgtac cgcagcgtgc cgtacacctt cgggccgcgc acggcgaaaa tcacctcttt
    15121 gatgccgccc agagtggcgt cggagagctc ggtgacttcg atcttccacc gtttgcgttc
    15181 gcagaaccgc gtatacatcc ggtagagatc cgcggcgaag agcgcggcct cgtccccgcc
    15241 cgtcccggcg cggatctcga cgatggcatc gcggtcgtcg agtggatccg ccggcaccag
    15301 gagctcggcc agctcggctt ccgtgctggc gacctccggg cgcaggcgct cgacgtccgc
    15361 ccgagccagc tgagccagct cggggtcttt gtcctggagc acctcccggg cctgtgcgag
    15421 ctcctcggtg aggcgttcga ggcgttggtt ggtctggcgg ataacgtcga ggcgggcatg
    15481 ctcacgcccc agctgcttca gcgctgccgg attccgggcc gtgctgggat cggccagaag
    15541 ccgggcgacc tcctccgccc gcgcgagcgc ctggcggagg cgggcctcca tcggtcagct
    15601 cgcggccttg cccgtcccgt acttctggcg gaaccgctcc acccggccgg cggtgtcgat
    15661 cagcttctgc ttgccggtga agtacggatg gcacttgcca cagatttcga cgttgatcgc
    15721 aggggtggtc gaccgcgtga tgaaggtgtt cccgcatgcg caggtcacca cggcccgctg
    15781 ataattcgga tgaatgcccg gtttcacgaa tacctgtcct gataaaggtt gcggctttgg
    15841 aagcgcggaa atataaacgg ggcatacagt tgccgacaag cacgccgaat tgacacccgg
    15901 acgccgctac cgtaagcttg cccgacaccc aagtcccgat tttttcaaag gatacgcgtg
    15961 ctggcgtccg aagtcctgcg caaggttccg ctgttcagcg agctgtccca ggccgatttt
    16021 cagcgggtgc tggatgtggc ccgggaacgc acgcacccca agaactccgt gatcctgttc
    16081 gaggacgacc ccggggatgc cctgtacgtc gtgtgcgggg gccaggtgaa ggtcgtgctc
    16141 atcggcgaag atggccggga ggtgattctc tcggtgctcg gcgagggcga gttcttcggg
    16201 gagatggcgt tgatcgacga cgagccgcgg tcggcccacg tcatcgccat ggaagactcg
    16261 accctggtgg tgctccgccg cgaggatttc gcgccgattc tctcgaccac ccccgccatc
    16321 gccatcgcgc tcctgcggga gctctctcgc cgcctgcggc gcgtggacga aaaagtcggg
    16381 tccctcgtcc tgctcgacgt gaatggccgt gtcgcgcgac tcctgctcga aatggcggac
    16441 gagaatggcg gcgagcggat cacccgtcgg ctcacccacc acaccatcgc acagatgatc
    16501 ggttcgtccc gcgaaacggt gtcgcgcacg atgcgcgaac tggtcgacaa gggactgatc
    16561 gaagtggggc gcaaggagat tctggtcaag aaccgccccg ccctggctga ggcggccggt
    16621 cgggggtgag gtaagtcacg accgggccgt gaggtgtcgg cttgttgtac gttgcagatt
    16681 tcccgtaact tagccctagg attacggtgt cagatcgcaa gtactcggtg acgttctggg
    16741 ggactcgggg atccatcccg actcccggcg cccacaccgc gcgctacggc ggaaatacgc
    16801 cgtgcgtcgc cgtggagggg gccgggggca agctggtcat cctcgatgcg ggcaccggca
    16861 tccgggcgct gggcgggaag ctcgtggaac ggcagaacgg cgcggtgacg gcggaaatcc
    16921 tgctgtcgca cgcgcactgg gatcacatcc agggccttcc ctacttcaag ccgttttttt
    16981 cccccgggaa tcaagtccgg atctggggat cacagcaggg tgaggtgtcc ctcgaggaca
    17041 ttctccggca acagatgcat ccgtcggtat tcccggtccc gctcgacgcg ctgtcggcgc
    17101 gtctgacggt ggagcatgtg ggatcgggcg agttcccggt gggcgagttc aaggtgcgct
    17161 ccacgcggct gcggcacccg ggcacgacgc tggggtatcg tttgaccccc gcggagggtg
    17221 gggcaagtct cgcatacgtg accgacaacg agctgggccc gggcggcaag tacgagacgg
    17281 gcccgggctg gcgccaggag ctcgtgggat tcttgaacgg cgtggacgtg ctgatccacg
    17341 atgcgatgta cacgccggac gagctgaaga cgcatgccgg ttggggccac tcgagctaca
    17401 ccgaagccgt ggcgctcgcc aaggaagcgg gagtgcaacg gctcgttctc tttcatcacg
    17461 agcccgagca ctcggacggc gcgatggatg acgtcctcgc cgcggcgcgc aaggaagcga
    17521 agaagctggg cggtccggga gaggtcctgg ccgcccgaga aggactgcag ctgacgttgt
    17581 gagtacccac aggggtcttg gacccgagga gcactctatg cgttcgcgtt cgatgctggc
    17641 cctgctcgcc ctggtcggtt tcgcggggcc cgcagtcgcg cagcaggcca cgacgaccga
    17701 cacccgtccg ggcgtcgccg tcttcgcctt cgaaaatggc ggatccatcg gcatgaagga
    17761 tccggcggat atgccggccc tgcagcgtgg cttcgcgggg atgatgatca acgagctcaa
    17821 ggcgaatgac gcctggcgcg tggtggagcg cgaggagatc cagaagctta ccgacgagca
    17881 gaatctcgac gcctcgggcc gcgtggatcc tcagactgcc gcgaagatcg gcaagatcgt
    17941 cggggccaag tacgcggtga cggggacgtt catcgccaac ggcaccaact ggcgcctcga
    18001 catccggctg atcaacgtcg agacgaccga aatcatgaag acggagtcgc agaccgcgtc
    18061 tcaggacaag ctgttcagca tcgtgacgga cgtggcgcag cgcctgatga aagacgccag
    18121 cctcccgccc ctgcccaagg acgtggctac ccagcgcatg agcaagcccg tcccgaccga
    18181 ggcgctgacc ttctactccc gcgcgctcct ctatcatgac cgcggcaaca aggagaaggc
    18241 cgtcgagatg tacaaggccg cgctgaacgt cttcccggat tacgtcccgg cgcaggaagg
    18301 cctgaagaag gagcagcagg gctgacccgc cggtgaattt catcggccgt ctgggcaaca
    18361 tcgtggcgcg ccggctctcc cagttggggg cgctggcgcg cttcggcttt ggcgccctcc
    18421 gggcgctggt caccctcccc gtcgccggcc gcgcggtcgc cttccgggtg accatgaacc
    18481 agatccgctt caccgccgtg cacgccgtcg ccctgatcgt cctgctctcc agcatcctca
    18541 gcttcctcgt gatctcgcag gccgcccgcg agctgggatc catcggcaag accgagtggc
    18601 tgggcaccct gatggtggtc gtgatcgtgc gcgagctggg cccgctcgtc accgcgctcg
    18661 ccgtcgcggg ccggtcgggg accgccatcg ccgcggagct cgccaccaat caggtgatgg
    18721 gagaagtccg ggcactggaa ggcatgggca tcgatccgcg gcagtatctc gtggtgcccc
    18781 gcttcgtggg cgggctggtc agcgtcttca tcctcatgat cctgttcgat gtcgtggccg
    18841 tcgccggggg gctgggggcc gcgaccttca acggcatggc gcccgggcgc tacatggacg
    18901 tcgtcctcca ggccctgacg ctgcgcgacg tctggctcac cgtggcgaag gcgctcgcgt
    18961 tcggcatcgt ggtcggcgtc gtgcccagct tctacggtct ggccgtccgc cgcggtccga
    19021 ccgaaatccc gatcgcggcc agccgggcga cggtctcgtc gatcgtcggc attttccttg
    19081 gcgccgcgct gttcggctga ccggtgaccg gccgcccgtc cgtcttcgaa ggcgccccgc
    19141 tggtcttcga tcaggtcctg atctcgccgc gcgcggcgac gccgttcagc ctcaccgtcg
    19201 cacggggcga aacggtggtc atcatcgccg aggaggacgt tccgctggga cccatggccc
    19261 tggcgctgga cccgccgcgc tcgggacgtg tcctcgtgct gggcaccgac ctgacggacc
    19321 tcccggaacg tgagcgcttg gccttccgcc gccgcgtggg ctaccttcca gccggcgacg
    19381 gcctgctgca aaatctctca ctccgcgaca acgtggccct gcccctgcgg ttcgggagca
    19441 cgaccggctc gaaggagatc gagggacggg tggaggtgat gttggccgcg gcccgcatcg
    19501 gccgcatcgg gggcctccgc cccgcgcagg cgaacgaaga agagcgccgg cgcaccgcgc
    19561 tggtccgcgc gctcgccttc gatccgtcat tgctgattct ggagcagccg ttcgatggtc
    19621 tctcgtcccg cgtggcgggt gagctgttga ccctggcgcg gggcggcgaa gaggtccaag
    19681 gcgggcgccg gtcggtgctg atcacctgcc aggagctccc gccgctcttg cacaaccggg
    19741 tggagcggat ctaccggctg gaccggcaag gcgtgctcga gctcgagccc gcgtagcgaa
    19801 ttcgaggagc cgtggaaacc aaccgtcagg atttcatcgt cgggctcttg atcgtggtcg
    19861 ccgtcgggct ggtgatcggg gcattgctcg cgaccagcgg gtgggggcag cggcgctacg
    19921 ccctgtatat gcgcgtcgcc aacgccgagg ggatcagcgc cgacacccgg gtcctggtgg
    19981 aaggcctgga gatcgggcgc gtggcgtcga tcgtgccgcg cgtcgacccc accagccggc
    20041 gcgtctcgtt catcgcgcgc ctctccctcg cgcaggaatt cgaaaacggc tcgaagctgc
    20101 tattgccgct cggcaccaag gcgtcgattg catcggtgtc ccagatctcg tccgcgtcgg
    20161 agatcgtcct gatcctgccc gactcgaccc atggggtcgc ccgcaccctt ggcgccggcg
    20221 acaccatcaa ttccgaccgg ccctcctcgg cgctcgattc gctgaaggtg gtggtgacca
    20281 gcctctccag tgatgtccgc gacgtgctgc tcagagcgcg cgacgccttg gtgggcgtgc
    20341 agcgcacgct ggcgtccgcg aacagcacga tccagaccat gacgccgagc ctgtcggccg
    20401 cggtcaccag tgtctcgtcg agcatggcgc ggatcgactc gatcactcgc cggattccgc
    20461 cgggccttgc ggactccgtc acggccacgc tggcgtcgtc gaaccgcttg ctggtccatt
    20521 tggactccgt ggtgcagagc gcgcaggtca tggccagcga gaaccggggc gacctccgcg
    20581 cgacggcgac gaacctcgcc gagctgtcgc gccagctgaa tcacttcgcc gacgcggtat
    20641 cccggcggcc ctaccgcttc ctgaccggtg tgaccccgct gccgccggac aaggcggatc
    20701 cccccaagga ttcgaccaag ccgtgaacgc gcgctacatc ctcctgcacc ccaccgggtg
    20761 gtcgccgacg atggtggtgg acgcgctcgc cagcgcccag atcgaggcgc gggcgatctc
    20821 cggaccagtc gagctcgagg tggatgaacg gcccacggtg ttcctgctgg acgcggggtc
    20881 ccgctcggcc tttccgctcg acgcccagcg ggcgttcgtc gatgccggag gcgccatcat
    20941 cgcgctggga cgggagggag agctggacgt cccggagccc ttcgccacgg agctcctcgc
    21001 ggcgttcgtc cgccatccgg tcggtcctcg ccagctcctg atcgcggtcc gcagcggcct
    21061 gcgggaggcg gcggcgcgaa gggaggcggc ccgcgccatg gcccaggcgg agcaacgctc
    21121 ccaggagatc ggcgaattca cccggatcgg cgcggcactc ggcaccgagc gggatctgaa
    21181 gacgctgctc gagctgatcc tcacgcaagc gcgccgcatc acccagtccg atgccggctc
    21241 gttgtatctg gtggaggccg ccgacagcgg ggccccgcgt ctgcgcttct ccctcgccca
    21301 gacgttctcc aagcccgagg cgccgttcgt cgagttcacc atcccgatgg atcgctccag
    21361 cctcgcgggc tacacagcgg tcaccggaga tcctctggtc atcgacgacg cctactatct
    21421 gcccccggac gtcgagtatt cgatcaaccg ctcgttcgac gagcggtacg gctaccggtc
    21481 gaagtcgatg ctcgtcatcc ccatgaagga tcaccgggac cagcgcatcg gcgtgctcca
    21541 gctgatcaac cggaagcgga actccgacgc ggtgctggga acggcggcgg acgtcgagcg
    21601 cgaggtggtg ccctactccc gccggagcgt ggagctcgtg accgccctcg cggcccaggc
    21661 ggcggtcgcc atcgaaaaca gccggctgta tcaggagatc gagcggctgt tcgagggatt
    21721 cgtccgggca tccgtgcaag ccatcgagca acgggacccg accacgttcg gtcattccgg
    21781 ccgggtggcc gactacacgg tctccctcgc ctccgtggtg gatcgcgccg gggcgggccc
    21841 gtacaaggcc gtccggttca cccgcgagca ggtccgggag atccggtacg cgggcctcct
    21901 gcacgatttc ggcaaagtcg gcgtccggga gcaggttttg gtcaaggcga agaagctcta
    21961 tccccccgat ctcgatctga tccgccagcg gcacgccttt gtgcgccgat cgctggagcg
    22021 ggatttctgg cagcggcgcg cggagttcct cgaggagcac ggcaagaaag ggtatccggc
    22081 attccaggag aagctggacg aggaacaccg gaaggccctc gccgatctcg accggttcct
    22141 ggagaccgtg ctgcagtcga acgagccgtc gctcctgccg gaacagagca agcacgatct
    22201 gtcgcaattt gcacggcgga cctacgccga cctggcgggt gctccacagc cactcctgac
    22261 cccggacgaa gtgcgatacc tgaccatccc gaagggatca ctggacgaag ccgagcgcct
    22321 ggagatcgag agtcacgtca cccatacctt caacttcctg aaggaaattc cctggacccg
    22381 tgagttgaag cgcatcccgg acatcgcgta cggccatcac gaaaagctcg acggccgggg
    22441 gtatccccgg aagatccagg gcgaggcgat cccgattcag acgcgcatga tgacgatctc
    22501 ggacatcttc gacgccctta ccgcgcagga ccgtccctac aaacgggccg tcccggcgga
    22561 gcgcgccctg gacatcatga agcaggaggt gacccaggga cagctggacg gtgagctcta
    22621 ccgcctcttc gtcgaaggaa gagtcttcga gccggccgcg gcgcgcgggg acgggtgaac
    22681 gcgacgctat tcgccgccct cgttttcggc ggctcctttc tggcgggact gctgggttcg
    22741 ttgacggggt tggggggagg gatggtgatc gtgcccctcc tcgcgatcgg gttcggtgtc
    22801 aatctccagt acgccatcgg ggcctccctc atcagcgtga tcgcgacgag ctcgggcgcg
    22861 gctgccgcgt acgtgcgtga gggctacacc aacatccgca tcggcatgtt tctcgaaatc
    22921 gcgaccacgg tgggcgccct cggcggcgcg ttcatcgccg ggatgatctc caccagcgcc
    22981 atcgccatcg ttttcgccgg ggtcttgctg tactccgcat ggctgtccgc gcgaccccgg
    23041 gtcgagcacg tcacccagga cctgtccgat cccctggccc gctccctgcg tttgcacggc
    23101 acctacccca cgccggatgg gctgcagccg tacggcgtcc agcgggtggc caccggtttc
    23161 tccctcatgt tcgtggcggg cattctgtcc gggctcctgg gcatcggatc gggcgcggtg
    23221 aaggtgctgg cgatggacca tgcgatgcgt ctgcccttca aggtttcgac gaccacctcg
    23281 aacttcatga tcggcgtgac cgccgcggcc agcgcggggg tctacctcag gcgcggctac
    23341 atcgacccgg tgctctcctt ccccgtcatg ctgggggtgt tggccggcgc cctcctcggc
    23401 gcccggatcc tcacgaagac acaccccaag catcttcgcc tcgtcttcag cgtcgtcatc
    23461 gtggcgctgg ccatcgagat ggtctatcgc gccatccagg ggacgatctg atggcccgtt
    23521 gggacgacac caaagtccag cacgccgtcg ggtc
//
LOCUS       DASUBP010000261         2709 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_336141, whole genome shotgun sequence.
ACCESSION   DASUBP010000261 DASUBP010000000
VERSION     DASUBP010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2709)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2709
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336141"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1725
                     /locus_tag="VFU75_09850"
     CDS             <1..1725
                     /locus_tag="VFU75_09850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467184.1"
                     /translation="SLDGGKTWVPVRGSPGGDDYHQPWISPDDSNTLIVASDQGTVIS
                     RNAKAADPRHLTWSSWLNQPTAQIYHVSVDPRFPYWVTGAQQDSGSVAVRSRGKFAQI
                     SMRDWEPIGAGGESGYTVGDPLHPGVIYGGSGSRYDLELNREVPGTTAPKAKEEGVTE
                     RHDWTQPLVLSPADPHALYYGTQFLYKSTDTARTWSEISPDLTRAQPGIPKNLDQTAA
                     ADQGNNQRGVIYAISPSPLQAAMVWVGTDDGLIQVTTDDGKTWKDVTPKELTPWSRVT
                     MVEASHTEANTAYASVDRHQLMDFDPYIYRTRDQGKSWQKITTGLPAGVYVHTVKEDL
                     HRAGLLFAGTERGAFVSFDAGDHWQPLQLNLPVTSVRDFQVYGNDLIVATHGRGFWVI
                     DDISVLRQIDDAVLKADAYLFQPADAIEVAQGDDNGTPLQKDEPQAENPPNGAYIDYY
                     LKSAPATPVTLEILDASGTSLETFSSAPAEPAAGGGGQGAGQPQGIPNTTALWRVPPA
                     PFSAAAGMHRVAWDPNAGRRRRGPRGGASQPPPPKLPGTFTAKLTVNGQSQTRSFMVR
                     PDPRIANE"
     gene            1747..>2709
                     /locus_tag="VFU75_09855"
     CDS             1747..>2709
                     /locus_tag="VFU75_09855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467185.1"
                     /translation="MSPRLFWLPCAALILAAAPAADGPFTIAQVLGYPYPSELVTAPS
                     GAAMAWVFNEHGVRNVWVAEGPDWKARRLTNGSADDGQELGQLQFAKDGKTIVYVRGG
                     DHDANWDAPPPDPDHSPVEPKVKIWTVPVAGGTPKVLTEGDSPLVSPRGDVVAFLKGG
                     QIWSIPVDGSKPASQLVFARGDNNSMVWSPDGSKLAFVSDRGDHSFITVFSDSTTPLQ
                     YLAPSTSQDVEPAWSPDGKQIAYVRLPGRGGPPRMMLQRTLSPWQIWVADVSTGAGHL
                     VWKSPLTLHASYGGAGLMWGDGGHLVFRNGADNWGHLYSVPVTGG"
BASE COUNT          475 a          897 c          918 g          419 t
ORIGIN      
        1 tcgctcgacg ggggcaagac gtgggtgccg gtacggggct ccccgggggg cgatgactac
       61 catcagccct ggatctcgcc cgacgactcg aacacgctga tcgtggcatc cgatcagggc
      121 accgtcatct cgcggaatgc gaaggccgcc gatccacgcc acctgacctg gagctcatgg
      181 ctcaaccagc cgacggcgca gatctatcat gtgtcggtcg atccccggtt cccgtactgg
      241 gtcaccggcg ctcagcagga ctcaggctcg gtggcggttc ggtcgcgcgg caagttcgcc
      301 cagatctcga tgcgggattg ggagccgatc ggcgccggcg gcgagagcgg gtacaccgtg
      361 ggcgatccgc tgcatcccgg cgtgatctac ggcggttccg gcagccggta cgacctcgag
      421 ttgaatcgcg aagtcccggg gacgacggcg cccaaggcga aggaagaagg ggtcacggag
      481 cggcacgatt ggacgcagcc gctggtgttg tcaccggcgg atccgcacgc gctgtactac
      541 ggcacccagt tcctctataa gtccaccgat acggccagga cctggagcga gatcagtccc
      601 gatctcacga gggcccagcc cggcatcccg aagaacctgg atcagaccgc cgcggccgat
      661 cagggcaaca atcagcgcgg cgtgatctac gccatctccc cgtcaccgct ccaggctgcg
      721 atggtctggg tcggtaccga cgacggcctc atccaggtga cgacggatga tgggaagacg
      781 tggaaggatg tgacgccgaa ggagctgacc ccctggagcc gggtcaccat ggtcgaagcc
      841 tcgcacaccg aggccaacac cgcctacgcg agcgtcgacc ggcaccagct catggatttc
      901 gatccctaca tctaccggac ccgggatcag ggcaagtcgt ggcagaagat cacgacgggc
      961 ctcccggcgg gggtctacgt ccacacggtg aaggaggacc tgcaccgggc cgggttgctc
     1021 ttcgccggga cggagcgcgg cgcgttcgtc tccttcgacg cgggggacca ctggcagccc
     1081 ctgcagctca atctgccggt cacgtcggtc cgcgacttcc aggtctacgg caacgacctc
     1141 atcgtcgcga cgcatggacg cgggttctgg gtgatcgacg acatcagcgt cctgcggcag
     1201 atcgacgatg cggtcctcaa ggccgacgca taccttttcc agccggcgga cgccatcgaa
     1261 gtcgcgcagg gggatgacaa cggcacgccc ctccagaaag acgagccgca ggcggagaat
     1321 ccgccgaatg gcgcgtacat cgactactac ctcaagagcg ccccggccac gccggtgacc
     1381 ctcgagattc tcgatgcctc cgggacatcg ctcgaaacgt tcagcagtgc acccgcggaa
     1441 ccggcggcgg ggggcggggg tcagggggcg gggcagccac aaggcattcc caacacgacg
     1501 gccctgtggc gagtgccgcc ggcgcccttc tcggcagccg cgggcatgca ccgtgtggct
     1561 tgggatccga atgccggaag gcggcgccgg ggtccacgtg gtggggcgag ccagcctcct
     1621 ccgcccaagc tgccgggcac gttcaccgcg aaactcacgg tgaatgggca gtctcagacg
     1681 cggagcttca tggtccgccc tgacccacgg atcgccaacg agtaggcaaa ccacgaggga
     1741 ctagggatgt cacccagact gttctggctg ccttgcgcag cgctgatcct ggccgctgct
     1801 cccgccgccg acgggccatt caccatcgcc caggtcctgg gctacccgta cccgagcgag
     1861 ctggtcaccg ctccctcggg tgccgcgatg gcgtgggtct tcaatgagca tggcgtgcgg
     1921 aacgtctggg tcgccgaggg tccggactgg aaggcacggc gcctgacgaa cggcagcgcg
     1981 gatgacggcc aggagctggg gcagctgcaa ttcgcgaaag acgggaagac gatcgtctac
     2041 gtccgtggtg gagatcatga cgcgaattgg gatgcgccgc cccccgatcc cgatcacagc
     2101 ccggtcgagc ccaaggtgaa gatctggacc gtgccggttg ccggcggaac gccgaaggta
     2161 ctgaccgaag gtgactcgcc cctggtgtca ccccgcggtg acgtcgtcgc gtttctcaaa
     2221 ggcgggcaga tctggtccat ccccgtcgac ggatcgaagc ccgccagcca gctggtgttc
     2281 gcccgcgggg acaacaactc gatggtctgg tctccggacg ggagcaagct cgccttcgtc
     2341 tcggaccgcg gcgaccacag tttcatcacg gtgttctccg acagtacgac gccccttcag
     2401 tacctcgcac cgtcgacctc tcaggacgtc gagccggcgt ggtctcccga cggcaagcag
     2461 atcgcctacg tccggctgcc cggacgcggt ggtcctccgc ggatgatgct gcagcgcacg
     2521 ctgtccccct ggcagatctg ggttgcggat gtgagcaccg gagcggggca cctggtgtgg
     2581 aagagcccgc tgactctcca cgcgtcctat ggcggcgccg gtctgatgtg gggggatggc
     2641 ggccacttgg tcttccggaa cggggccgac aactggggac acctctattc ggtgccggtg
     2701 accggtggg
//
LOCUS       DASUBP010000262        13582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1112724, whole genome shotgun sequence.
ACCESSION   DASUBP010000262 DASUBP010000000
VERSION     DASUBP010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 13582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13582
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112724"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..582
                     /locus_tag="VFU75_09860"
     CDS             <1..582
                     /locus_tag="VFU75_09860"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEU5467186.1"
                     /translation="PERVAAEAAEPLGIPVFPVLSYGVTPYFREFPGSVSLKVETHLA
                     VIRDILDSLAHSGFRRIMICNGHGGNGAAQQLAQQWSAEHPKCRVMFHNWWNAPKTWA
                     KVQSIDKVASHGSWMENFPWTRIPGVEVPKTQRPMVDMDKVRLLDPVAMRAYIPDGNY
                     GGFYQRPDEDVLALWQVAVEETRALLDGPWGAS"
     gene            567..1628
                     /locus_tag="VFU75_09865"
     CDS             567..1628
                     /locus_tag="VFU75_09865"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: protein motif:HMM:TIGR00745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEU5467187.1"
                     /translation="MGGFLSPTPMRGLIWGAGAIGGTLGAYLARAGHDITFVDNVADH
                     VAAINRGGMRISGPIAEFVVPVPAFTPETLRGTWDIVMLATKAHHTEPAVRALAPHLA
                     AKGCVVSVQNGLNELAIASAVGEGRTVGSFVNFGADYLEPGHIMYGGRGTVMIGEAFG
                     PDGPERITHRVLAIRDAWRDFDDRATATANIWGYLWGKEAYGAMLFATALTNESIADC
                     LARLEYRGVYIALAREMLAVGAARHVTPEAFDGFDPAAYLPDAPVGAAERSLEALVAH
                     NRKSAKTHSGIWRDLAIRKRPTEVDAQLGIVVTLGREAGVPTPLAAKVVDLIHEIERG
                     ERPQSLETLDALVRVMPTA"
     gene            1625..2392
                     /locus_tag="VFU75_09870"
     CDS             1625..2392
                     /locus_tag="VFU75_09870"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEU5467188.1"
                     /translation="MNIRFDGKTVIVTGAAHGFGRAISLAFAERGARVWACDVAEDEL
                     HETHRLCQDAGGDCEVKPVDVRDREAVGRFVGEAAESAGCVHVLVNNAGGVLGQVGKP
                     LEGVTPVEWQAIFDVNTTGAFFMSQAVAPGMKAKRYGRIVNISSGAGLGVSLTGIQAY
                     ASAKAAQIGLTRQLAHELGPWGITVNNIAPGFVRSNQTTEKQWESYGAEGQRALVDRI
                     ALKRLGTPDDIAFGVLFFASEQAGWITGQVLSIDGGK"
     gene            2389..3774
                     /locus_tag="VFU75_09875"
     CDS             2389..3774
                     /locus_tag="VFU75_09875"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF006579.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEU5467189.1"
                     /translation="MSDAAVLAPVLARLEAAHDRILAELVEFARIPSVSTDPKRAGDL
                     RAAAEWVAAKLSAAGPLAVKTMPTPGASVVYAEWLGAPGAPTALIYGHYDVQPEDPVD
                     RWLSPPFEPAVREGRLYARGVSDDKGPMLIPIKVAEAFFAERQRLPLNLKFLIEGEEE
                     VGSKHLEGFVTGHREMLRADAVISADGAMWRIDEPSLNVSSRGLVALELTLRGPAKDL
                     HSGRHGGSVPNPLHAMARLIASLHDQNGRVATSGFYDRVCELSAAERAEIAALPFDEG
                     KYLAQVGARAAVGEAGYSTLERQWTRPTLEVNGLWGGYQGPGSKTVVPSEAHAKITCR
                     LVPDQDPDEVARLVIAHLERHTPPGAVLSIQPGGHGARPASIPRDHPVLKAAESALAE
                     TYGVRPIFVRMGGTLPVAETFRKVLGLDTVYFSFSTGDEDFHAPNEFFRVARLHQGLA
                     AWARLWERLGR"
     gene            3771..5198
                     /locus_tag="VFU75_09880"
     CDS             3771..5198
                     /locus_tag="VFU75_09880"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEU5467190.1"
                     /translation="MSIQQEEARVEAQSASLKKELGVRDLALTQILFIIGLTWIGVAG
                     KLGSAHVVMWLLAVVLFYLPLAAVVIWLNQLMPLEGGLYQWAKLGFNQTVGFMVAWNL
                     WLYVMLFASELGLTVATYLSYALGPGAAWMTSSVWFVSLASALAIALLAGLAFVGLSL
                     AKWVHNAGGVFMITIFGVLILLPLLGLATGHLSSFHPFRATLPDVSLFNLNILGKLSV
                     GAFAGFEYMAIFAGETHSPASAVRKSVYIAAPVIALFFILGTATVVAYIPTNDIDLIG
                     PLPQVLRVGFGPFGIAGLLVTIAILMTLVMRVSQISVAFSAVTRLPMVAGWDRMLPAW
                     FSRLHPTYRTPVNSIAIVAVFSFAMSVVSLVGVGQAEAFQLIFNAGGIFYALTYVVMF
                     AIPLIGLRGVTPRPPWWVRGAAASGLVITLLYTVLSVFPIIQVASVAGFALKITAVIV
                     LLNIVGFGILVAARRRSNVAANTGA"
     gene            5201..7297
                     /locus_tag="VFU75_09885"
     CDS             5201..7297
                     /locus_tag="VFU75_09885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HEU5467191.1"
                     /translation="MNGRKGGFAVAVLALALSAVAFATRSPSAGTLAGEWDVYIALSA
                     KPKFGFEGWRRMGFAHFASGDSGNVGWLKRRTGEPMLTVTSVTNSGDSVVLTQDTRVS
                     MRAAWHGDTLVGVQWIGDRLVDRRFRLVRRDTPGVVEHDYQVWKMPASDSQYAVTEDT
                     LVFMPTRDGARLATYIARPVGPGPFGVVLQRTPYRRVLHPAGRWWAARGYIFVAQHVR
                     GRDQSSGDVADFGDYTTDIQDGYDAVEWAAKLPGANGKVGMIGHSDEGKLVWLAAVSN
                     PPHLAAISPTAASPDGWVVVPYAAMAFGPINVDWACLMRSRTMDPNTAELDIGEAITH
                     LPLANLPQRLGCGDVPLWDRWVAHPTLDAYWRKHAATTYIANMKAPVLSMAGWHDDSR
                     GPTYYTDALLRVKNHPKWHIVMGVGAHKGVDYVAGDFGPNARYAYRDLQIRWFDHYLL
                     GKQNGVDTLPAFDDFVIGDNTWRQEREWPLARAKATNWYISSGGNAQTSAGDGVLDTL
                     APTGAAADTFTYNPGDPTPYLIDSRELEESLNEDYTALNASRKDALVYTSKPLTKAIE
                     VTGELTATVWAASDRKDTDWTVMLLDVWPDGHAERVQDGLVRARFRKGMDKEVPLVPG
                     QVERYDVDVWFTSMVFEPGHRLRVSIASALFPKYDRNLNTGGNNERDSVFVVAHQRVL
                     HDATHATHITLPIVPR"
     gene            complement(7350..8390)
                     /locus_tag="VFU75_09890"
     CDS             complement(7350..8390)
                     /locus_tag="VFU75_09890"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HEU5467192.1"
                     /translation="MRALIVGPPAAERGALASQAREKGYVVETASTVSEAVVMLNSGE
                     FDLVLLSAPLPDVEAARFFEAAAVVETRRRRSVESLTHEMDVARSIQRDFLPDALPTS
                     RGVQLDAALHPARQVSGDFYDAFAMPPSNTVVLVVGDVCDKGVGAALFMALFRSLIRA
                     SADPVEGGAIQMIGGRRTMVRQSIEAASPAELLTRVAGFTNDYIARLHGRTNMFATVF
                     LGALDTGTGRLDYVNAGHEPAIVIAPSGGLRELRPTGPALGLMPDQLFKTGEATLEPG
                     DSLIAFTDGLVEARNLAGEAFGAERLRSVLQANHLSAKALVKGVLDAVQAFTGHAEPH
                     DDVTLLAASRSS"
     gene            complement(8387..9403)
                     /locus_tag="VFU75_09895"
     CDS             complement(8387..9403)
                     /locus_tag="VFU75_09895"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HEU5467193.1"
                     /translation="MTSLLAGLVSVLPVFVFLGALVLIDSYKLVRLRAILLSVAAGLI
                     AGLAAYGANVWLRPALSLDWERYSRYVAPVVEESLKAAFVIYLLKRNKVGFVVDAAIH
                     GFGIGTGFAVLENLYYLQANPGATIWTWVVRGFGTAIMHGGATALMAMLTKTLQSRTN
                     VFRLYLALPGLALAMVLHSFYNHFALHPLLATGLILLIFPMLATLVFERSERETKAWL
                     GTGFDTDQELLRAVQTGQVSGTPAGRYLTTVQNRFPAEVIVDMMCLLRLHAELAIRAK
                     GVLMMREAGFDAPPDPGVKDKLEELKYLEKSIGKTGLRALHPFVHHSTRDLWQLTVLD
                     GEGR"
     gene            complement(9400..12366)
                     /locus_tag="VFU75_09900"
     CDS             complement(9400..12366)
                     /locus_tag="VFU75_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467194.1"
                     /translation="MPRHRFILIAALLACAGVCAPSRATAQFETIRTSDMHLVYTSPL
                     QAYMVPQVARSFENSLKFHCTLFGYDPGGPINVLMHDLWHYGNAGASPLPENHITIGI
                     EPYSHEYESAPAPERMVSSLNHEMAHIVTVDKATGSDRFYRSLFFGKVTPNPEMPLSM
                     LYGYLTTPRWYAPRWYLEGIAVYMETWMNGGVGRAIGPYDEMVFRTLVRDSSRIFDFV
                     GLESEGTTIDFQVGVNSYLYGTRFVSYLGLRYGNDSLLAWFNRTPGSKRYFSSQFRHV
                     FGRSLQDEWSRWIDWERQWQTANLSSIRRHPITAYRPLTDHALGSVSRAFYDSTTKAV
                     YLGIRYPGQVAHLARIDVATGAITNLKEILGASGYSVTSLAFDPASRILFFTTNNANW
                     RNLYSYDVASGHSRLLLRNARIGDLAFDRADSSLWGVRHDNGFSTLVRLPAPYHEWNQ
                     IQTLPYGEDLFDLDLSPDGTTLTGSMSEINGSQKLVLLNVAALRAKTNPKPETLDDFG
                     EWPPSNFVFSPDGKFLFGSSYYSGVSNIYRYDLTRRVTEPLSNTETGFFKPVPLPGDS
                     VLVFRYSARGFIPSLIPNVVPDSVSAIKFLGNEIAEKRPDVQTWIPKRDSSLNLDSLL
                     ATARPYHSLAHLAMNSMYPVVEGYQNAAGDRAAALGMRANLSDQVGATALNLTASYAP
                     DQGLSSFERLHLRAEFLSWNWKIMAAMNRADFYDLFGPTKVSRRGYSLAVQYRGDLLL
                     DDPKSVTYTLRVAGYSGLSTLPEFQNVAAPFSKLLSFSGNIEYGSLRRSLGAVEDEVG
                     STAGATLHGNYVNGTLFPQVDVSLSHGFLLPINHSSLWFRGAAGSTLHGNRSNAFAQF
                     FFGGFGNNWVDYRDVKQFRALESFPGIGIDDAGGSNFGKAQMEWVLPPVRFREVGIPS
                     FYLQWASLSLFTTGLVTDMDQASLRRKLVSAGAQLDFRLVTLSHLESTMSFGVAVAGE
                     SGVPVHSVGMFSFKIM"
     gene            complement(12443..>13582)
                     /locus_tag="VFU75_09905"
     CDS             complement(12443..>13582)
                     /locus_tag="VFU75_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467195.1"
                     /translation="CSPHVNACAWRSMKEFETMLTRDQVELIQQEIDGANTPVASAAL
                     RSLLEQNPEARAYAAELRRVSGALARVGERQSPSHLRQSILDALPQPVRASPGGIVAW
                     VGQQLNLVGELLEGAIMNKKSLLIGSAAVAVVIVAAGLITGFPPIGGSSGTIGGVEKA
                     TRYHGHEMTDADISLKNPEIQALFQNDQVLRLVQSDAFHQLMASDAFRTVMATDAFNK
                     IMQSDAFRQIMASDAFRSLSASDAFRQLNNTAAYRTLQAQDAFRNLNQADAMRQLNQN
                     AAYQQLQAIDAFRTVMATDAFRQVMANDAFRQIMAQDAFRQILANDAFRSILANDAFR
                     SILATDAFRTLSAAEAFRTLSQSQALSQDFMREAASQQVSAQAKN"
BASE COUNT         2246 a         4311 c         4740 g         2285 t
ORIGIN      
        1 cccgagcggg tggccgcgga ggccgccgaa ccgctcggca ttcccgtgtt cccggtgctg
       61 tcctacggcg tgaccccgta cttccgcgaa ttccccggct ccgtctcgct caaagtcgag
      121 acccacttgg ccgtgatccg cgacatcctc gacagcctgg cccactcggg cttccggcgc
      181 atcatgatct gcaacggcca cggtgggaat ggcgcggccc agcagctcgc gcaacaatgg
      241 agcgccgagc acccgaagtg ccgggtgatg ttccacaact ggtggaacgc gccgaaaacc
      301 tgggccaagg ttcagagcat cgacaaggtg gcgtcgcacg ggtcgtggat ggagaacttc
      361 ccgtggacga gaatccccgg ggtcgaggtt ccgaagaccc agcggcccat ggtggacatg
      421 gacaaggtcc gcctgctcga ccccgtcgcc atgcgggcgt acatcccgga cggcaactac
      481 ggcgggttct accagcggcc cgacgaagat gtcctcgccc tctggcaggt cgccgtggaa
      541 gagacgcgcg cgctgctcga cggtccgtgg ggggcttcct gagccccacg ccgatgcgag
      601 gcctgatctg gggtgccggc gccatcggcg gtacgctcgg cgcctacctc gcccgcgccg
      661 gtcacgacat cacgttcgtc gacaacgtgg ccgaccacgt cgcggccatc aatcgcggcg
      721 gcatgcggat ctcgggcccg atcgcggaat tcgtcgtgcc cgtgcccgcg ttcacgccgg
      781 agaccctgcg cggcacgtgg gatatcgtga tgctcgcgac caaagcccac cacacggagc
      841 ccgccgttcg ggccctggct ccgcacctcg ccgccaaagg ctgtgtcgtc tccgtccaga
      901 acggactcaa cgagctcgcg atcgcgtctg cggtgggtga agggcggacg gtcgggtcct
      961 tcgtgaattt cggcgccgac tacctggagc cgggccacat catgtacggt ggccgcggga
     1021 cggtgatgat cggcgaggcc ttcggcccgg acggccctga gcggatcacc caccgggtcc
     1081 tcgccattcg ggacgcgtgg cgcgacttcg acgaccgggc caccgcgacg gcgaacatct
     1141 ggggctacct gtgggggaaa gaagcgtacg gcgcgatgct gttcgcgacc gccctcacca
     1201 acgagtccat cgccgattgc cttgcccgcc tcgagtaccg cggcgtctac atcgcactgg
     1261 cccgcgagat gctcgcggtg ggggcggcgc ggcacgtgac gccggaggcg ttcgacggct
     1321 tcgaccccgc ggcgtatctc cccgacgcgc ctgtcggcgc cgccgagcgg tcactcgagg
     1381 ctctcgtggc ccacaaccgc aagtcggcca agactcactc aggcatctgg cgggacctcg
     1441 ccatccgcaa gaggcctacc gaggtggacg cccaactggg gatcgtggtg acgctcggcc
     1501 gtgaggcggg ggtgccgacg ccgctggcgg cgaaggtcgt cgatctcatc cacgagatcg
     1561 agcgcggcga gcgcccgcag tccctcgaga cgctggacgc gctcgtccga gtgatgccta
     1621 ccgcatgaac atccggttcg atgggaagac ggtcatcgtg accggtgcgg cgcacggctt
     1681 cggccgggca atcagcctcg ccttcgccga gcggggcgcg agggtctggg cgtgtgatgt
     1741 ggccgaggac gaactccacg agacccacag gctttgccag gacgcgggtg gtgactgtga
     1801 ggtgaagccc gtggacgtgc gcgaccggga ggcggtcgga cggttcgtgg gcgaggccgc
     1861 ggaatccgcc ggatgcgtgc acgtgctggt gaacaacgcg ggcggagtgc tggggcaggt
     1921 ggggaagccc ctcgaaggag tcacaccggt cgagtggcag gccatcttcg acgtcaacac
     1981 caccggcgcg ttcttcatgt ctcaggcggt ggcgccggga atgaaggcga agcgctacgg
     2041 acgcatcgtc aacatctcta gtggagccgg gctcggtgtg agcctgaccg ggattcaggc
     2101 gtacgcgtcg gcgaaagcgg cgcagatcgg cctcacgcgt cagctggcgc atgagctggg
     2161 tccctggggg atcaccgtca acaacatcgc cccggggttc gtccggtcga accagaccac
     2221 ggaaaagcaa tgggagtcgt acggcgctga aggccagcgg gcgctggtcg accggattgc
     2281 gctcaagcgg ctgggaacgc cggacgacat cgccttcggc gtgctgttct tcgcgtccga
     2341 acaggccgga tggattacgg ggcaggtgct ctccatcgac ggtggcaagt gagcgacgcg
     2401 gcggtcctgg cgccggtgct cgcgcgcctc gaggcggcgc acgaccggat cctggccgag
     2461 ctggtggagt tcgcccggat accgagcgtg agcaccgacc caaagagagc cggggacctg
     2521 cgggcggcgg ccgaatgggt ggccgcgaag ctctcggcgg ccgggcccct cgccgtgaag
     2581 acgatgccga caccgggtgc atcggtggtg tacgccgagt ggctcggggc acccggcgct
     2641 ccgacggcgc tgatctacgg gcactatgac gtgcagccgg aagatccggt ggatcgctgg
     2701 ctgagtccgc ccttcgagcc ggcggtgcgg gagggccggc tctacgcccg cggtgtgtcc
     2761 gatgacaagg gcccgatgct cattcccatc aaggtcgcgg aggcgttctt cgcggagcgg
     2821 cagcgtctgc ccctcaacct caagttcctc atcgagggcg aggaagaggt cgggagcaag
     2881 catctggaag gattcgtcac tgggcaccgc gagatgctgc gggcggatgc cgtgatctcc
     2941 gcggatgggg cgatgtggcg gatcgacgag ccgtcgctga acgtctcgag ccgcgggctg
     3001 gtggcgctcg agttgacgct gcgcgggccg gccaaggatc tgcactcggg gcgccatggc
     3061 ggcagcgttc cgaatccgct gcatgcgatg gcgcgcctca tcgcgtcgct gcacgaccag
     3121 aatggccgcg tagccacttc gggcttttat gatcgggtct gcgagctctc agccgccgaa
     3181 cgagcggaaa tcgcggcgct gcctttcgat gaagggaagt atctcgcgca ggtgggcgcg
     3241 cgggccgcgg tcggcgaagc cggctactcg accttggagc gccagtggac gcggcccaca
     3301 ctcgaggtca acgggctctg gggaggctac cagggcccgg ggtccaagac cgtggtgccg
     3361 agcgaggcgc atgcgaagat cacgtgccgg ctggtgccgg accaggatcc cgatgaggtc
     3421 gcgcgtctcg tcatcgccca cctcgagcgt cacacgcctc cgggtgcggt gctctcaatc
     3481 caacctgggg ggcatggcgc gaggccagcc agcattcccc gtgaccatcc cgttctcaag
     3541 gctgcggagt cggcgctggc ggagacgtac ggcgtgcgcc cgatcttcgt gcggatgggc
     3601 gggacgttgc cggtagcgga gacgtttcgg aaagtcctcg gactcgacac cgtctatttc
     3661 tcgttctcga ccggggacga ggacttccac gcgcccaacg agttcttccg ggtggcacgg
     3721 ttgcatcagg ggctcgccgc gtgggcccgg ctctgggagc gcctcggccg atgagcatcc
     3781 agcaggaaga agcccgagtc gaagcgcaga gcgcttctct gaagaaggag ctgggggtcc
     3841 gggatctggc gctcacgcag atcctgttca tcatcgggct cacgtggatc ggcgtcgccg
     3901 ggaagctcgg atcggcgcac gtcgtgatgt ggctcctcgc cgtggtgctg ttctatctcc
     3961 cgctggcggc cgtggtgatc tggctcaacc agctgatgcc gctcgagggc gggctgtacc
     4021 agtgggcgaa gctggggttc aaccagaccg tcgggttcat ggtcgcctgg aacctgtggc
     4081 tttacgtcat gctgttcgct tcggagctgg gtctgaccgt ggcgacctac ctctcgtacg
     4141 cgctgggccc cggcgcggcg tggatgacga gcagcgtgtg gttcgtctcc ttggcgtccg
     4201 cgctggccat cgccctcctg gccgggctgg cgttcgtggg cctgtcgctc gcgaaatggg
     4261 tccacaacgc cggcggcgtg ttcatgatca ccatcttcgg ggtcctcatc ctgctcccgc
     4321 tccttgggct ggccacgggg cacctctcct cgttccaccc gttccgtgcg acgttgccgg
     4381 acgtctccct gttcaatctg aatattctgg gcaagttgtc ggtcggcgcg ttcgccggct
     4441 tcgagtacat ggcgattttc gctggtgaga cccattcgcc ggccagtgcg gtgcggaagt
     4501 cggtgtatat cgccgctccc gtcatcgcgc tgttcttcat tctgggtacg gcgacggtgg
     4561 tggcctacat cccgaccaac gacatcgatc tcatcgggcc gctgccccag gtgctgcgcg
     4621 ttggattcgg tcccttcggc atcgccgggc tgctcgtgac catcgccatc ctcatgacgc
     4681 tcgtgatgcg ggtgtcccag atcagcgtcg ccttcagcgc cgtgacccgt ctgcccatgg
     4741 tggcggggtg ggaccggatg ctgccggcgt ggttcagccg cctgcacccc acctaccgga
     4801 cccccgtcaa ctccatcgcg atcgtggccg tgttctcctt cgccatgtcg gtggtgagtc
     4861 tggtgggtgt ggggcaggcc gaagcgttcc agctcatctt caacgcgggc gggatcttct
     4921 acgcactcac ctatgtcgtc atgttcgcga tcccgctgat cgggctccgc ggcgtgaccc
     4981 cgcgcccgcc gtggtgggtc cggggcgcgg ccgcctcggg actcgtgatc accctgctgt
     5041 atacggtgct gtcggtcttt ccgatcatcc aggtggcgag cgtcgccgga ttcgcgctca
     5101 agatcaccgc ggtgatcgtg ctgttgaaca tcgtgggatt cgggatcctg gtcgccgccc
     5161 gtcggcggtc caacgtcgca gccaacacgg gagcatagtc atgaacggaa ggaagggtgg
     5221 gttcgccgtc gccgtcctgg cgctggcctt gagtgcggtc gcgttcgcga cgcgttcccc
     5281 ctccgccggg actctggcgg gtgagtggga tgtctacatc gcgctgagcg ccaagcccaa
     5341 gttcggattc gaaggctggc gccggatggg attcgcgcac tttgcgagcg gcgactccgg
     5401 gaacgtgggc tggttgaagc gccgtaccgg cgagccgatg ctgacggtga cgtcggtgac
     5461 caactcaggc gattcggtgg tgttgacgca ggatacccgg gtgtcgatgc gggccgcttg
     5521 gcacggcgat accctggtgg gggtccaatg gatcggcgac cgcctggtgg accgccgctt
     5581 ccgtctggtg cgccgcgata ccccgggtgt ggtggagcac gattaccagg tgtggaagat
     5641 gccggcgtcc gattcgcagt acgccgtcac cgaagacacc ctggtgttca tgccgacgcg
     5701 cgacggcgcc aggctcgcga cctacatcgc gcggccggtg ggtccgggac ccttcggcgt
     5761 ggtgctccag cggacaccgt accgccgcgt gctccatccg gcgggccggt ggtgggcggc
     5821 gcgcggatac atcttcgtgg cgcagcacgt gcgcgggcgc gaccagtcga gcggcgatgt
     5881 ggcggacttc ggagactaca cgaccgacat tcaggacggg tacgatgcgg tggagtgggc
     5941 cgcgaagctc cccggagcga acggcaaggt cgggatgatc ggccattcgg acgaagggaa
     6001 actggtctgg ctcgcggccg tctcgaaccc tccgcatctc gcggcgatct cgcccaccgc
     6061 ggcgtcaccc gatggatggg tggtcgttcc gtatgcggcg atggcgttcg ggccgatcaa
     6121 cgtggactgg gcctgcctga tgcgttcgcg gacgatggat ccgaatacgg ccgaactgga
     6181 catcggggag gcgatcaccc acctgccgct cgccaacctg ccgcagcggc tggggtgcgg
     6241 tgacgtgccg ctgtgggacc gctgggtggc acaccccacg ctggacgcgt attggcggaa
     6301 gcacgcggcc accacctaca tcgccaacat gaaggcgccg gtgctgtcca tggcgggttg
     6361 gcacgacgat tcgcggggac cgacctacta cacggacgcc ctgcttcggg taaagaacca
     6421 tcccaagtgg cacatcgtca tgggcgtggg ggcccacaag ggcgtggact acgtcgcggg
     6481 agacttcgga cccaacgccc gctacgccta tcgcgatctc cagattcgat ggttcgacca
     6541 ctacttgttg ggcaagcaga acggcgtcga taccctcccg gccttcgatg atttcgtgat
     6601 cggcgacaac acgtggcgcc aggaacgcga gtggccgctg gcgcgggcga aggcgacgaa
     6661 ctggtacatc tcctcgggcg gcaacgcgca gacgagcgcc ggggatggtg tgctggatac
     6721 cctcgcgccg accggtgccg cggcggacac cttcacctac aatcccggcg accccacgcc
     6781 gtacctgatc gactcccgtg agctggagga gtcgctcaac gaggactaca cggccctcaa
     6841 cgcgagccgg aaggacgcgc tggtctatac gtccaagccg ctgaccaagg ccatcgaggt
     6901 gacgggggag ctgacggcca cggtgtgggc ggccagtgac cggaaggaca cggactggac
     6961 cgtgatgctc ctcgatgtct ggcccgacgg gcacgccgag cgggtgcagg atgggctggt
     7021 gcgggcgcgg ttccggaagg ggatggacaa ggaggtcccc ctcgtgccgg ggcaggtcga
     7081 gcgctacgac gtggacgtgt ggttcacgtc catggtgttc gagcccggcc atcggctccg
     7141 cgtgagcatc gcctcggcac tattccccaa gtacgaccgg aacttgaaca cgggcgggaa
     7201 caacgagcgg gactcggtct tcgtggtggc gcatcagcgg gtgttgcacg acgctaccca
     7261 cgccacgcac atcacactgc cgatcgtgcc tcggtaagca ggggtcaggg gtcagggggc
     7321 aggtcccctg acccttgctc gtcccggggt tacgaactcc gggacgcggc gagcagtgtc
     7381 acgtcgtcgt gcggctcggc gtgccccgtg aacgcctgaa cggcgtccag cacgcctttg
     7441 acgagcgcct tggcggacag gtgattcgcc tgcaggacgc tccgcaaccg ctctgcgccg
     7501 aaggcctcac ccgccaggtt gcgcgcttcc accaggccgt cggtgaacgc gatgaggctg
     7561 tcaccgggct ccagcgtcgc ctccccggtc ttgaacagct ggtcgggcat gaggcccagc
     7621 gcgggacctg tgggccgcag ctcgcgcagg ccgccactcg gcgcgatcac gatcgccggt
     7681 tcgtggcccg cgttgacgta gtccaggcgc ccggtgccgg tatcgagtgc tcccagaaag
     7741 accgtggcga acatgttggt gcgcccgtgc aggcgggcga tgtagtcgtt cgtgaaaccg
     7801 gccacgcggg tcaagagctc cgccggactc gcggcctcga tcgactggcg taccatggtg
     7861 cggcggccac cgatcatctg aatcgcacca ccctcgacgg ggtcggcgga agcgcggatg
     7921 agtgagcgga agagggccat gaacaatgcc gcgcccacgc ccttgtcgca gacgtcgccc
     7981 accacgagca cgaccgtgtt ggagggcggc atcgcgaagg cgtcgtagaa gtctccggac
     8041 acctggcgtg ccggatgcag ggcggcatcg agctggacgc cgcgcgaggt ggggagggcg
     8101 tcgggcagga agtcccgctg gatgctgcgg gccacgtcca tttcgtgcgt gaggctctcg
     8161 acactccggc gccggcgcgt ctccaccacg gccgccgcct cgaagaacct ggcggcttcc
     8221 acgtccggga gcggcgcgga gagcaggacg aggtcgaact ctccgctgtt cagcatcacg
     8281 accgcctcgc tcaccgtgct cgcggtctcc accacgtacc ccttttccct ggcttgcgac
     8341 gccagggcgc cacgctcggc ggctggcggc cccacgatca gcgctctcat cttccttccc
     8401 cgtcgagcac ggtcagttgc cagaggtccc gggtactgtg gtggacgaat gggtgcagcg
     8461 cgcgcaatcc cgtcttgccg atgctcttct cgaggtactt gagctcttcc agcttgtcct
     8521 tcacacccgg atcgggtgga gcgtcgaatc cggcctcgcg catcatcagg acacccttcg
     8581 cgcggatggc cagctccgca tgcaaccgga gcaggcacat catgtcgacg atcacctcgg
     8641 cggggaagcg gttctggacg gtcgtgaggt aacgccccgc gggcgttccc gacacctggc
     8701 ccgtctgcac ggcccgcagg agctcctgat cagtgtcgaa ccccgtgcct agccaggcct
     8761 tggtctcgcg ttcgctccgc tcgaacacca gggtggcgag catcgggaag atgagcagga
     8821 tcaggcccgt ggccaggagg ggatgcaggg cgaaatggtt atagaaggaa tggagtacca
     8881 tggccagggc gagtccgggc agggcgaggt agagccggaa cacgttggtc cgggactgga
     8941 gcgtcttggt gagcatcgcc atgagcgcgg tggcgccgcc atgcatgatc gccgtcccga
     9001 atccgcgcac gacccaggtc cagatcgtgg ccccggggtt cgcctgcaaa tagtagaggt
     9061 tttcgagtac tgcgaagccg gtaccgatgc cgaagccgtg gatcgcggca tccacgacga
     9121 atccgacctt gttccgcttc aataagtaga tgacgaacgc cgccttgagg gactcctcca
     9181 ccacgggggc gacgtaccgg gaatagcgct cccagtcgag actcagcgcg gggcggagcc
     9241 agacattcgc tccgtaggcg gcgagtccgg cgatcagtcc cgcggcgacc gacaggagga
     9301 tcgcccgcaa gcggacgagc ttgtagctgt cgatgaggac cagtgcgccg aggaagacga
     9361 agaccggcag gacactgacc aggcccgcga gaaggctcgt cacatgatct tgaaggagaa
     9421 catgccgacg gagtgcacgg ggacccccga ttcgccggcc acggcgactc cgaacgacat
     9481 ggtggactcg aggtgggaga gtgtcaccag gcggaagtcg agctgggcgc cagcgctcac
     9541 gagtttgcgc cgcagcgacg cctgatccat gtcggtgacg agtccggtcg tgaacaggga
     9601 caggctggcc cactggagat agaagctggg aatccccact tcacgaaagc ggacgggcgg
     9661 cagcacccac tccatctggg cctttccgaa gttcgagccg ccggcgtcgt cgatcccgat
     9721 ccccgggaag ctctcgagag cccggaactg cttcacgtcc cggtagtcca cccagttgtt
     9781 gccgaacccg ccgaagaaga attgcgcgaa ggcgttgctc cggttcccgt gcagtgtcga
     9841 tcccgccgca ccccggaacc agagggaaga gtgattgatc gggagcagga agccgtggga
     9901 cagactcacg tcgacctggg ggaagagggt gccgttgacg tagttgccgt gcagcgtggc
     9961 acccgcggtg gaccccactt catcctccac ggctcccaag gaccgccgga gcgacccgta
    10021 ttcgatgttg ccggagaacg acagcagctt cgagaaggga gcggcgacgt tctggaactc
    10081 gggcagggtc gagaggcccg agtaccctgc tacgcgcagg gtgtacgtca cgctcttcgg
    10141 atcgtccagc agcaggtcgc cccggtactg caccgcgagc gagtagccgc gccggctcac
    10201 cttcgtgggc ccgaagaggt cgtagaagtc ggcgcggttc atggcggcca tgatcttcca
    10261 gttccacgac agaaactcgg cgcgcaggtg gaggcgctcg aacgacgaca gtccctgatc
    10321 gggggcgtac gacgcggtca ggttcagcgc ggtggcgccg acctgatcag agagattcgc
    10381 acgcatgccg agggcggcgg cgcggtcgcc ggccgcattc tggtatccct ccaccaccgg
    10441 gtacatgctg ttcatcgcca gatgcgcgag ggagtggtac gggcgtgccg tggcgagcag
    10501 ggagtccaga ttgaggctcg agtcgcgctt gggaatccac gtctgcacgt ccgggcgctt
    10561 ttcggcgatc tcgttgccga ggaacttgat cgcgctcacg ctgtccggca cgacgttggg
    10621 gatcaacgat gggatgaatc cgcgcgcgct gtaccggaac accaggaccg agtcgccggg
    10681 caggggcacg ggcttgaaga agcccgtctc cgtattggac aggggctccg tgacgcgccg
    10741 ggtgaggtca taccggtaga tgttcgagac cccggagtag tacgacgagc cgaagaggaa
    10801 cttgccgtcc ggcgagaaca cgaagttcga cggtggccac tcgccgaaat cgtcgagcgt
    10861 ctcgggcttg gggttcgtct tggcccggag cgccgcgacg ttcaacaaca ccagcttctg
    10921 gctcccattg atctcggaca tcgatccggt cagcgtcgtg ccgtcgggcg agagatcaag
    10981 atcgaagagg tcctcgccat agggcagggt ctggatctgg ttccactcgt ggtagggcgc
    11041 ggggagacgg acgagcgtcg agaatccgtt gtcgtgccgg acgccccaga gcgagctgtc
    11101 ggcccggtcg aacgcgagat cgccgatccg cgcgttccgc aagaggagcc gggagtggcc
    11161 actggccaca tcgtacgaat acaggttccg ccagttggcg ttgttcgtgg tgaagaacag
    11221 gattcgtgac gcggggtcga atgccagtga agtcaccgaa taccccgagg cgccgaggat
    11281 ttccttgagg ttggtgatgg ctcccgtcgc gacgtcgatg cgggcgagat gggcgacctg
    11341 acccggatag cggatcccca ggtagacggc cttcgtggtc gagtcgtaga acgcgcggga
    11401 taccgagccc agggcgtggt ccgtcagcgg ccggtaggcc gtgatcgggt ggcggcggat
    11461 ggacgacaga ttcgccgtct gccactgccg ctcccagtcg atccagcgcg accactcgtc
    11521 ctggagggac cggccgaaga cgtgccggaa ctgtgacgag aaatagcgct tgctgccggg
    11581 ggtccggttg aaccaggcga gcaggctgtc gtttccgtac cgcagaccca ggtagctgac
    11641 gaagcgcgtc ccgtagaggt agctgttgac gcccacctgg aagtcgatcg tcgtgccttc
    11701 ggactcgagc ccgacaaaat cgaaaattcg ggagctgtcc cgcaccagcg tgcggaagac
    11761 catttcgtca taggggccga tggcgcgtcc gacgcccccg ttcatccagg tttccatgta
    11821 cacggcgatc ccctccaggt accaccgtgg cgcataccac cgcggcgtgg tcaggtagcc
    11881 gtagagcatg gagaggggca tctccggatt cggcgtgact ttcccgaaga acagcgagcg
    11941 atagaaccgg tcggatcccg tcgccttgtc cacggtcacg atgtgggcca tctcgtgatt
    12001 gagcgaggag accatccgct ccggcgccgg ggcggactcg tactcgtggg agtagggctc
    12061 gatcccgatc gtgatgtggt tttcgggcag ggggctcgcc ccggcgttgc cgtaatgcca
    12121 gaggtcgtgc atcagcacat tgatcggccc gccgggatcg tagccgaaca gggtgcaatg
    12181 gaacttcagg gagttctcga agctccgggc gacctgcggc accatgtacg cctgaagggg
    12241 tgacgtatag accaggtgca tgtcgctggt ccggatggtc tcgaactggg cggtggccct
    12301 cgaaggggcg cacaccccgg cgcaggccag aagggccgcg atcagaatga atcgatgtcg
    12361 tggcatggag gggggagaaa ctacaggagg atcccggccc gcgttagagc gaaccgggat
    12421 ccccgggaaa acggtcagtc ggtcagttct tcgcctgcgc cgacacctgc tgcgaagcgg
    12481 cttcgcgcat gaagtcctgg gacaacgcct ggctctgcga cagcgtgcgg aatgcctcgg
    12541 ccgcagagag cgtccggaat gcatcggtgg ccagaatcga gcggaacgcg tcgttggcca
    12601 gaatgctccg gaaagcgtcg ttggccagga tctgacggaa cgcgtcctgg gccatgatct
    12661 ggcggaacgc gtcgttcgcc atcacctgcc ggaacgcgtc ggtggccatc acggtgcgga
    12721 acgcgtcgat cgcctggagc tgctggtacg ccgcgttctg gttcagttga cgcatcgcgt
    12781 ccgcctgatt caggttccgg aacgcatcct gcgcctgcag cgtgcgatag gccgcggtgt
    12841 tgttgagctg gcggaacgca tcgctggcgc tcaacgaacg gaacgcgtcc gaggccatga
    12901 tctgacggaa cgcgtcgctc tgcatgatct tgttgaacgc gtcggtcgcc atcaccgtgc
    12961 ggaaggcatc gctggccatc agctggtgga acgcgtcgct ctggaccaga cgcagcacct
    13021 ggtcattctg gaacagggcc tgaatctccg gattcttgag ggagatgtcg gcatcggtca
    13081 tctcgtgacc atggtaccgg gtggccttct cgacgccgcc gatcgtgccg gaactgccgc
    13141 cgatcggggg gaagccggtg atgagccccg cggccacgat cacgacggcc acggcggcgc
    13201 ttccgatgag cagtgacttc ttgttcatga tcgctccctc taagagttca ccgacgagat
    13261 tcagctgttg accgacccat gccacgatcc cgccgggtga ggcgcggacc ggttgcggca
    13321 gggcatccag gatcgactgc ctcaggtgcg acggcgactg ccgctcgccc acccgcgcga
    13381 gggccccgct gacgcggcgc agttcagccg cgtacgcccg ggcctccgga ttctgctcca
    13441 gcagggaccg gagcgccgca ctcgccacgg gagtgttcgc gccgtcgatc tcctgctgga
    13501 taagctccac ctggtcacga gtcagcatcg tttcgaactc cttcatcgag cgccacgcgc
    13561 aggcgttcac gtgcggtgaa ca
//
LOCUS       DASUBP010000263         3357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_827065, whole genome shotgun sequence.
ACCESSION   DASUBP010000263 DASUBP010000000
VERSION     DASUBP010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3357
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_827065"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1182)
                     /locus_tag="VFU75_09910"
     CDS             complement(139..1182)
                     /locus_tag="VFU75_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467196.1"
                     /translation="MRTHLRSLTMLLVAASLADAAAPTVTPQASGTTQRLQAVSPVTD
                     RIVWASGTGGTYVRTTDGGDHWRAGVVPRADSLEFRDVAAMDSMNAWLLAAGPGPASR
                     IYRTTDGGATWTQQFVNTDSAAFFDCFAFWDASHGIAVSDGVRGHFPILVTDDGGAHW
                     TLRPTSETPAADSGEGAFAASGTCIVTGPGGRAWIGTGASPTGGGRVVATADFGKTWT
                     SQQTPVQHGTTTTGIGSLAYRDATHAFAGGGDINPRAPQTVRVARTDDGGRTWVPITP
                     PGFPGGIYGLAAVPETEGLVAVGPGGAAYSPDGRSVWLPLDSLSYWSAGFASPHNGWL
                     VGPKGRIAKVVLP"
     gene            1238..>3357
                     /locus_tag="VFU75_09915"
     CDS             1238..>3357
                     /locus_tag="VFU75_09915"
                     /inference="COORDINATES: protein motif:HMM:NF039501.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HEU5467197.1"
                     /translation="MTSLLLLALTASLAAGDTTPPAMLAGTVRDTISGQPVGLALIRV
                     PGRAVSVITDDEGHFRLYVDPGPVHLEVRRIGYRPRSLVVAAPDTAVVLYILPVPVGI
                     AGITVRPVEDPAIEIMRRVIAHKQNVLSHLHDYSYGADIKFVVRNQSVPVDSPASILA
                     VTESRTTAYWERPDHYQETIVARRQTGNVDPNRNLVSVGEIVNFAKDRIDLRKYSVVS
                     PIATDALNHYDYRMLDTTASAGGRIFTIAIVPRSEASPLFVGVLTVADSTYEVRSIDV
                     GANSALRFTFVSNLRYHQQLADQGGGRWMPSEIRLTGEIHLAVPIPGIPAHLSFEHIA
                     SLAGFAFDQGNRPKGMGEYRVVVADSADRPDSSWAEAIPLTPTEREAWMRIDSIATTP
                     ATLGHRMAMGLGAAVFLAANPTFFHYNRVDGAYLGAGGDWSPGPHWVVGTSAGYGFGA
                     ERWQESASVSRMLSEDRQIWIGAGAFDQTAHRPTMVSSDYNPTFRNFLSRIDPLDYYR
                     ERGARAFVSGKLWRGTRLELSFVNATQSSLAVTTDYALGVPKARALRPNEPIDGGNLR
                     AVGATMVYDSRPLLRSKGRDYRLRQLVQTRVTASAEVAAHDVLGGDFSYRRYVLRVER
                     IQRTLNFGVTSITAVGGIATGWVPPQRYFTVDFGRELYTYQPNGFNTLNGNNFSGTRA
                     AMLTINHDFDRLLFAKSHIPGIRSL"
BASE COUNT          536 a         1109 c         1132 g          580 t
ORIGIN      
        1 gattcactgg ctgccgatct gcacctgatt ccagtggcga cctttaagca tggggtcgtc
       61 tggatgaccg ctaagtcgct aggtcgctaa gtcggtaagg tggtcactta gcgtcttagc
      121 gacttagcgt cttagcgact aagggaggac gaccttggcg atacgccctt tcggaccgac
      181 caaccagccg ttgtggggac ttgcgaatcc ggcactccag tagctgagcg aatcgagcgg
      241 aagccagacg ctccggccat ccggcgagta cgcggcccct cccgggccga cggcgacgag
      301 accctcagtc tccggcacgg ctgcgagtcc atagatgccc cccggaaatc caggtggcgt
      361 gatcggcacc caggtccggc cgccgtcgtc cgtccgcgcc acgcggaccg tctgcggggc
      421 ccgggggttg atgtccccgc cgccggcgaa cgcgtgcgtc gcgtcgcggt aggccaggga
      481 cccgatgccc gtggtggtgg tgccatgctg aacgggtgtc tgctgagacg tccaggtctt
      541 cccgaagtcg gcggtggcca cgacccgacc gccgcccgtc ggactggcgc cggtgccgat
      601 ccacgcccgg ccgccggggc cggtgacgat gcacgtaccg ctcgcggcga aggcgccctc
      661 tcccgaatcg gcggccggag tttcactcgt cggcctgagg gtccagtgcg cgccgccatc
      721 gtcggtgacg agaatcggga agtgcccgcg cacgccgtcg ctcacggcga tgccgtggct
      781 cgcgtcccag aaggcgaagc agtcgaagaa cgcggcggag tcggtgttga cgaactgctg
      841 ggtccaggtg gcaccgccat cggtcgtgcg gtagatccgc gacgccggcc cgggccccgc
      901 cgcgagcagc catgcgttca tcgagtccat cgccgccacg tcacggaact ccagggagtc
      961 cgcccgcggc acgacccccg cgcgccaatg gtcgccccca tcggtggtcc tgacgtaggt
     1021 gccgcccgtg ccgctcgccc agacgatccg gtccgtgacg ggactgacgg cttgcaggcg
     1081 ttgcgtggtt ccgctggctt ggggggtcac ggtcggggcg gcggcatccg ccagcgacgc
     1141 ggcgaccagg agcatcgtga gggaacgcag gtgtgtgcgc atagggaaca gaaagtaacg
     1201 ctggaacagg aggttggtct ggacgtatga ttcgcccgtg acttcgctgc tcctccttgc
     1261 gctgacggca agcctcgccg ccggtgacac gacgcccccc gcgatgcttg ccggtacggt
     1321 gcgtgacacg atctccgggc agccggtggg gctcgcgctc atccgggtgc cggggcgagc
     1381 cgtctccgtc atcaccgacg acgagggtca tttccggttg tacgtggacc cggggcccgt
     1441 ccacctcgaa gtgcgccgca tcgggtaccg cccgcgcagt ctggtagtcg cggcacctga
     1501 cacggccgtg gtcctctata tcctgccggt tcccgtcggc atcgcgggga tcaccgtccg
     1561 tcccgtggaa gacccggcca tcgagatcat gcgacgggtg attgcccaca agcagaacgt
     1621 gctttcgcac ctgcacgact acagctacgg ggccgacatc aagttcgtcg ttcgcaacca
     1681 gagcgttccc gtcgattctc ccgcgtccat tctcgcggtg accgagtccc ggaccacggc
     1741 atattgggag cgtcccgatc actatcagga aacgatcgtc gcgcgacggc agacggggaa
     1801 cgtggatccg aaccggaacc tcgtctcggt cggggagatc gtgaactttg ccaaggaccg
     1861 gatcgatctc cggaagtact cggtcgtttc accgatcgcc accgacgcac tgaaccacta
     1921 cgactaccgg atgctggaca ccacggcgtc ggcgggcggc cggatcttca cgatcgcgat
     1981 cgtgccgagg tccgaggcct caccgctgtt cgtcggggtc ctgacggtgg ccgactcgac
     2041 ctacgaggtg cgcagcatcg acgtcggcgc gaacagcgcc ctgcggttca ccttcgtttc
     2101 gaaccttcga tatcaccagc aactcgccga ccagggcgga ggccgctgga tgccctccga
     2161 gatccggctc acgggggaga ttcacctcgc ggtgcccatc cccggtatcc ccgcgcacct
     2221 gagcttcgag cacatcgcgt cactcgccgg gttcgcgttc gatcagggga accggcccaa
     2281 gggaatgggt gagtatcgtg tggtggtggc ggacagtgcc gaccgtccgg attcgtcctg
     2341 ggccgaggcg attccgttga cgccgacgga gcgggaggcg tggatgcgga tcgactccat
     2401 cgcaaccact ccggcgaccc tggggcatcg catggccatg ggtttgggag cggcggtgtt
     2461 cctcgcggcg aaccccacgt tcttccacta taatcgcgtc gacggggcat acctcggcgc
     2521 cgggggtgat tggagtccgg gtccgcattg ggtcgtgggc accagcgcgg gctacgggtt
     2581 cggggcggag cggtggcagg agagcgcgag cgtgagccgc atgctctccg aggaccggca
     2641 gatctggatc ggcgcgggag cctttgatca gacggcgcac cgtccgacga tggtgtcgag
     2701 cgattacaat ccgacgttcc gcaactttct ctcccgcatc gatccgctgg actactaccg
     2761 cgagcggggc gcgcgcgcat tcgtctcggg aaagttgtgg aggggcactc ggctcgagct
     2821 ttccttcgtc aatgcgaccc aatcgagcct ggcggtgacc accgattatg cactcggggt
     2881 tcccaaggcg cgggcgcttc gtcccaacga gccgatcgac ggcgggaatc tgcgggcggt
     2941 cggcgcgacg atggtatatg actcccgccc gctcctgcgg tcgaaggggc gcgattaccg
     3001 gctccgtcag ctggtgcaga cccgcgtcac ggcgtcggcg gaagtcgcgg cccacgacgt
     3061 actgggcggc gatttctcct accggcgcta cgtcctccgg gtcgagcgca ttcagcggac
     3121 gttgaacttc ggtgtcacgt ccatcactgc ggtggggggc atcgcgaccg gatgggtacc
     3181 gccgcagcgg tatttcacgg tggacttcgg ccgggagctc tatacctatc agcccaacgg
     3241 gttcaatacc ctgaacggca acaacttctc cggcacgcgc gcggcgatgc tcaccatcaa
     3301 tcatgacttc gatcgcctgc tcttcgcgaa gagtcacatc ccggggattc gatcgct
//
LOCUS       DASUBP010000264         4372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_336357, whole genome shotgun sequence.
ACCESSION   DASUBP010000264 DASUBP010000000
VERSION     DASUBP010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4372
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336357"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..657
                     /locus_tag="VFU75_09920"
     CDS             <1..657
                     /locus_tag="VFU75_09920"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HEU5467198.1"
                     /translation="DGAGRRAIHRALTDHAGTPGVLPGYRLDGKTDPQIVRELLEQGG
                     HSSPHDQAVIEEICTQYVVNLREELKRPGYVTRAMPGVPALLEALVPMEEAGDVMVGL
                     LTGNVVGGAELKLEAAGIGFGRFAVGAFGLDGHHRPDLPPVALARASERLGRPVRGAD
                     TWIIGDTPADVACGRPVGARALAVATGSYRVADLEVTGAELVLADLSDTGRVLEGLGL
                     "
     gene            654..1214
                     /locus_tag="VFU75_09925"
     CDS             654..1214
                     /locus_tag="VFU75_09925"
                     /inference="COORDINATES: protein motif:HMM:NF014038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HEU5467199.1"
                     /translation="MSVKDEIEVKARVPDLKSFEAALDRAGATVEFAGEMTDHRFDRK
                     EKLAEKDEVLRLRIYRPQNGETRGELCWKGPVETKGEYRHRPEIELGVSDPWTALTLL
                     DRLGYTESMRIERMITVYHLGQATLRVERYPEMDILVEVEGDPEAIEKAIAATGLARG
                     EFLPESLPQFVSRYEKRTGRQARLAT"
     gene            1211..1933
                     /locus_tag="VFU75_09930"
     CDS             1211..1933
                     /locus_tag="VFU75_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467200.1"
                     /translation="MTVRVPELAPALGRLIVPRRTEPLWIPLDDIADELATRVIELGG
                     EARRLTAEAAPQDRVLDALGRTAWLAAWEHAVRRTADRVADALDDQIERIGKAVRMPR
                     RKRRLRKLTSAERRSLTARLSAGGAEFVKALDDLARVSSKVRGEATAGGVDDETHHVW
                     QDALQTAARRLEAAWLALEDAIRTERKRWDPELAALAQWRPSLWPVLVTWAIGAGALL
                     WLGLILGGYVDAPVWLAQLLHF"
     gene            1933..2337
                     /gene="acpS"
                     /locus_tag="VFU75_09935"
     CDS             1933..2337
                     /gene="acpS"
                     /locus_tag="VFU75_09935"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEU5467201.1"
                     /translation="MLNALGIGVDLVEVDRARRMLAEKGSHVFDRLLTPAEAEYCRSR
                     PDPAEHVAVRLAAKEAVYKALQGSDAARGIGWREIEVVREQDGRPQVTLHGLAAQRAK
                     ELKAAQVMLSLSHTHQAAVAVAIVVALPTESL"
     gene            2394..4121
                     /locus_tag="VFU75_09940"
     CDS             2394..4121
                     /locus_tag="VFU75_09940"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c/FTR1 family iron permease"
                     /protein_id="HEU5467202.1"
                     /translation="MFVLALAGSLPAQGKDSSRVLAARRMVSAISLAAKEYRNGVPAA
                     GGKITAPEEVAESRLFLDQAEKNVATLPDSLREPVGLLVANLRASLDRPSAPPPDSVS
                     QWADALVMRITRAFGNTLNQYPAVPPSVARGKVVFAKACASCHGLAGRGDGPGGRGLN
                     PPPANLADPAEMRTVTPLDVYRKVSIGVPGTAMPRFEDDLTDADRWAVAAYVSTLQPD
                     SKLVAAFAAVRSRLDTAVANRSADQAFDAYLAFEQVETDVRARNAPLAGQLEDEFANL
                     RNRAAAGASAGELAGIHEQILAGLERAEREVADRDSRTNLFLESLGITLREGFEAILI
                     IGALMAFLAKAGAVERRRDVAWGAWLAVLASLATFAGVELLFEVTSNQREALEGWTML
                     LATVVLFYVSYWLLSKVEADKWNSFVRDKVKSALSSGSRWALAGVAFLAVYREGFETI
                     LFYKALLTSGNTGGVVAVVAGLAVGCVGLVLIYLAINLFGMRLPLRPFFAVTGGLLYY
                     MAFVFAGKGIAELQNAGVVGMRPVEWGPRLPALGIYPTAQSLWAQGVLVVLALAAVVW
                     ALARSQRPA"
     gene            complement(4118..>4372)
                     /locus_tag="VFU75_09945"
     CDS             complement(4118..>4372)
                     /locus_tag="VFU75_09945"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEU5467203.1"
                     /translation="HGMGWLFGCYLILSGAERFLIEFLRAKDDRLLGPFTLAQATALI
                     IVAVGFYLTKKWQVPDGTGADIPHLLARKPEPPQQAPVAT"
BASE COUNT          664 a         1393 c         1596 g          719 t
ORIGIN      
        1 gacggtgcgg ggcggcgagc cattcatcgc gcgctgaccg atcacgccgg gacaccgggt
       61 gtcctccccg ggtaccggct cgacggcaag accgacccgc agatcgtccg ggagctgctg
      121 gagcagggag ggcacagttc cccccacgac caggcggtga tcgaggaaat ctgtacccag
      181 tacgtcgtca acctccggga ggagctgaag cgcccgggct acgtgacccg cgcgatgccg
      241 ggggttccgg cgctgctcga ggcgctggtg ccaatggagg aggccgggga cgtcatggtg
      301 ggtctgttga cggggaacgt ggtcggggga gcggagctga agctggaagc ggcgggaatc
      361 ggcttcggcc ggttcgccgt gggggccttt ggactcgatg gccatcatcg tcccgacctt
      421 cctccggtgg cccttgcccg cgcctccgag cgtctgggcc gtccggtgcg cggggccgat
      481 acctggatca tcggtgatac gccggcggac gtcgcgtgcg gccggccggt gggtgcgcgg
      541 gccctcgccg tcgcgaccgg ctcctaccgc gtggccgatc tcgaggtcac cggggccgag
      601 ctggtgctcg ccgacctgtc cgacaccggg cgggtgctcg aagggctggg gttgtgagcg
      661 tgaaggacga aatcgaagtg aaggcccggg tgcccgacct caaatcgttc gaagccgcgc
      721 tggaccgcgc gggggccacg gtggagttcg cgggagaaat gaccgaccac cggttcgacc
      781 gcaaggagaa gctcgccgaa aaagatgagg tgctccgctt gcggatttac cggccgcaga
      841 atggcgagac gcgtggagag ttgtgctgga aggggcccgt ggaaacgaag ggtgagtacc
      901 ggcaccggcc ggagatcgag ctcggggtct cggatccgtg gactgcgctg acgttgctcg
      961 accggctggg gtacacggag agcatgcgga tcgagcgaat gatcacggtc taccacctgg
     1021 gacaggcaac gctgcgcgtc gaacggtatc cggaaatgga catcctcgtc gaagtggaag
     1081 gcgatccgga ggccatcgag aaggcaatcg ccgccaccgg actcgcacgc ggggagttcc
     1141 ttcccgagtc gctcccccaa ttcgtgagcc gctacgagaa gcggaccggc cgccaggcga
     1201 ggctggccac gtgaccgtcc gagttcccga actggccccg gcgctcggcc ggctgatcgt
     1261 gccgcgccgc acggaaccgc tgtggatccc tctggacgat atcgccgatg agctggcgac
     1321 ccgggtgatc gagctggggg gggaggcccg ccggctgacc gccgaggctg ctccacagga
     1381 ccgcgtcctc gatgccctgg gccggacggc ctggctggcc gcgtgggagc acgccgtccg
     1441 ccgcactgcc gaccgtgtcg ccgatgccct cgatgatcag atcgagcgca tcggcaaggc
     1501 ggttcgcatg ccgcgacgga aacgccggct tcggaagctc acgagcgcgg aacgccgctc
     1561 gctgacggca cgcctgtcag cggggggcgc cgagttcgtc aaggcactcg acgatctggc
     1621 gcgggtcagc agcaaggtgc gaggcgaagc gacggcgggc ggtgtcgatg acgagaccca
     1681 ccacgtctgg caggatgccc tgcagacggc ggctcgacgg ctcgaagccg catggctcgc
     1741 gctcgaggac gcgattcgca ctgagcggaa acgctgggat ccggagctgg ccgcgctggc
     1801 gcagtggcgt ccctcgctct ggccggtgct ggtgacctgg gcgatcggcg cgggcgcatt
     1861 gctctggctg gggctcattc tcggtggcta tgtcgacgcg ccggtctggc tcgcgcaatt
     1921 gctgcacttc tgatgctcaa cgcactcggc atcggcgtcg acctggtgga agtggaccgc
     1981 gcgcggcgga tgctggcgga aaaggggtcg cacgtcttcg accggctgct cacgccggcg
     2041 gaggcggagt attgccgcag ccgccccgat cccgcggagc acgtggccgt gcggttggcg
     2101 gcgaaagagg cggtctacaa ggcgctgcag gggagtgacg cggcgcgtgg catcggctgg
     2161 cgggaaatcg aagttgtacg cgagcaggat ggacgtccgc aggtgacgct gcacggtctc
     2221 gccgcgcagc gggcgaaaga actgaaggcc gcgcaggtga tgttgtcgct gtcgcacacg
     2281 caccaggcgg cggttgcggt cgccatcgtc gttgcattgc cgacagaatc gctctagtat
     2341 tcgtctcacc ctagctgaga atcattctca tctatcccca tctcacgatt ccagtgtttg
     2401 tcctcgccct cgctggctcg ctcccggcgc aggggaagga ctcgtcgcgg gtcctggccg
     2461 cgcgccggat ggtctccgcg atttcgctcg ccgcaaagga gtaccgcaac ggcgtgcctg
     2521 cggcaggtgg caagatcacc gcaccggagg aagtggccga gtcacggttg ttcctggacc
     2581 aggcggagaa gaacgtcgcc acgctcccgg attcgcttcg ggagccggtg ggactcctgg
     2641 tcgccaacct ccgtgcgtcc ctggaccgac cttcggcgcc gccccccgat tcggtctccc
     2701 aatgggcgga cgcgctcgtg atgcggatca cccgggcctt cgggaatacg ctcaaccagt
     2761 acccggcggt cccgccgtcc gtcgcccggg gcaaagtggt attcgcgaaa gcgtgcgcgt
     2821 cgtgccatgg gctcgcgggc cggggtgacg gtcccggagg tcgggggttg aatccgccgc
     2881 ccgccaatct cgccgacccg gccgagatgc ggaccgtcac cccgctcgac gtctaccgca
     2941 aggtatccat cggtgtcccg ggtaccgcga tgccgcgctt cgaagacgac ctcacggacg
     3001 ccgaccgctg ggccgtggcc gcttacgtct ccacgctcca gcccgattcg aagctggtcg
     3061 ccgcgttcgc ggcggtccgc tcccggctcg atacggcggt ggcgaaccgg tccgccgacc
     3121 aggccttcga cgcgtacctc gcgttcgagc aggtggagac cgacgtgcgc gcgcggaacg
     3181 cgcctctggc ggggcagctc gaggacgagt tcgcgaatct ccgaaaccgg gccgccgcgg
     3241 gtgcgtcagc gggcgagctc gccgggatcc acgagcagat cctcgccgga ctcgagcggg
     3301 ccgagcggga ggtggcggac cgggactcgc ggacgaatct gtttctggag tccctcggga
     3361 tcacactccg ggaaggattc gaggcgatcc tcatcatcgg ggcgctgatg gccttcctgg
     3421 ccaaggcggg ggcggtggag cgccggcggg atgtggcgtg gggggcctgg ctcgcggtgc
     3481 tcgcgtcgct cgcgaccttt gccggcgtcg agctgctgtt cgaagtgacg tcgaaccagc
     3541 gggaggccct cgaaggctgg acgatgttgc tcgcgaccgt ggtgctcttc tacgtcagct
     3601 attggctgct ctcaaaggtc gaagcggaca agtggaattc gttcgtccgc gacaaggtaa
     3661 agagcgcgct gtcctcggga tcgcggtggg cgttggccgg ggtggcgttt ctcgcggtgt
     3721 atcgcgaggg atttgagacc atcctgttct acaaggcgct cctgaccagc ggcaacacgg
     3781 gtggtgtcgt ggcggtcgtg gccgggcttg ccgtaggatg cgtcgggctc gtcctgatct
     3841 atctcgcgat caatctcttc ggcatgcggc ttcccctccg gccattcttc gcggtgacgg
     3901 gggggctcct ctactacatg gccttcgtct ttgcgggcaa gggaatcgcg gagctgcaga
     3961 atgccggcgt ggtgggcatg cggcccgtcg agtggggtcc gcgtcttccc gctctgggga
     4021 tctatcccac ggcccagtcc ctgtgggcac agggggtctt ggtggtcctg gctctcgccg
     4081 cggtcgtgtg ggccttggcc cggagccagc gcccggctta ggtcgcgacg ggcgcctgct
     4141 ggggaggttc cggcttccga gccagcagat gggggatgtc ggcgcccgtc ccgtccggaa
     4201 cctgccattt cttcgtcaga tagaatccga cagccacgat gatcagcgcc gtcgcctggg
     4261 ccagcgtgaa aggtcccagc aggcggtcat ccttcgcgcg caggaattcg atcaggaacc
     4321 gctccgcccc ggacaggatc aggtaacacc cgaaaagcca gcccatgccg tg
//
LOCUS       DASUBP010000265         5833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_77958, whole genome shotgun sequence.
ACCESSION   DASUBP010000265 DASUBP010000000
VERSION     DASUBP010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5833
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77958"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VFU75_09950"
     CDS             <1..365
                     /locus_tag="VFU75_09950"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF015146.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEU5467204.1"
                     /translation="LTKILAAIQLPREQVYICNIVKCRPPENRVPQFDEISSCLPYLW
                     RQIELVRPKVILAMGGTAAQSLLDAKQALGAMRNRVHRFRGIPVVVTYHPAALLRNPN
                     WKKPTWDDVRFARRLLDA"
     gene            328..1749
                     /gene="dnaB"
                     /locus_tag="VFU75_09955"
     CDS             328..1749
                     /gene="dnaB"
                     /locus_tag="VFU75_09955"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEU5467205.1"
                     /translation="MTSASPAVSSTPDRTFGGRQPPWSNEAEQAVLCAMMLSQDAALS
                     AVEVLDDTMFYREAHRVLFRAMSSLTEKREVIDPVTLGDELTRRGDLDRIGGMEYVGA
                     LIDVVPTAANVTFHAKIVRDKAVLRRLVEAATEIIQDVYDSGSESGEVLDNAEHRVFQ
                     VAQLRRSEEFSRLKELVWPAMERIEQLQGGAGAVTGVASGFLDLDRLTAGFQKSDLVI
                     IAARPSMGKTAFVLNVVQHAAIEHNIGVAVFSLEMSKDALVQRLLCSEGLVDAQRLRR
                     GALRDDDYPKLARAAGLLGTAPIWIDDSAALTPLAMRSKARRLKAEHDIGLVVVDYLQ
                     LMAGPTNVESRQQEISYISRALKGLAKELNVPVLALSQLSRAPEQRGGEHRRPQLSDL
                     RESGAIEQDADLVCFIYRQEFYDGPVDPKSDENIEGLAELIVGKQRNGPTGTVKLYFK
                     KEYTRFDNYTERSGGPPGEQFGG"
     gene            1742..3109
                     /gene="radA"
                     /locus_tag="VFU75_09960"
     CDS             1742..3109
                     /gene="radA"
                     /locus_tag="VFU75_09960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEU5467206.1"
                     /translation="MAKPHSVFRCTECGAEQPKWAGRCDSCSAWNTLVEEPVLEKRRQ
                     PSATSRQQALVGSAPPVRLGEVTGNSRERWKTGIAEFDFVLGGGIVPGSLVLVGGEPG
                     IGKSTLLLQAAAQLEKNGVRTLYVSGEESPDQLRLRADRLGADAHAVHALGETRLESI
                     VAHAAALNAQVVFVDSIQTTYTEELEGAPGNVGQVRECAARMMRFAKDAGPAVVLVGH
                     VTKGGGIAGPRTLEHIVDTVLYFEGEATLDHRILRAVKNRFGSVDEIGVFRMTAAGLE
                     AVPDPAALFLAGRQTGVSGSAVTALMEGTRPLLVEIQALASKAGFGTPQRVATGFDQR
                     RLAVLLAVLERRGGLPFSDLDVFVNVTGGVRLLEPSTDLAVTAALVSSIHDRPAPADA
                     LFLGEVGLGGEIRPVAGIERRLAEATRQGFRRAFLSARSRVRVEGLDVVGLEHIGELA
                     RRLAA"
     gene            3081..3788
                     /gene="ispD"
                     /locus_tag="VFU75_09965"
     CDS             3081..3788
                     /gene="ispD"
                     /locus_tag="VFU75_09965"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: protein motif:HMM:NF013308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEU5467207.1"
                     /translation="MSSRGASLPDVGVVIVAAGAGLRAGPGEPKQFRPILGVPMLLRA
                     LRPFTSHPEVSQVVAVLPRAFVEHPPDWLAKVMGDRLILARGGESRTDSVRSGVKALR
                     DDCRLILVHDAARPFPVRATIDAVIARARSGVGAVAAVPLSDTVKEVKGDARVTRTVP
                     RTGLWRAQTPQGFPRTMLVDAFNRLKLADPPPTDDGELCERAGFPVDVVPDSPHNFKV
                     TTPDDFRIADALARELR"
     gene            3785..4441
                     /locus_tag="VFU75_09970"
     CDS             3785..4441
                     /locus_tag="VFU75_09970"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HEU5467208.1"
                     /translation="MTGVVPFDSPQSASSSTPQVAAHLRGGGLVAYPTETVYGLGSLA
                     RAEPVNALSRLKGREPGKPFLLLVSNRQMAESYGLAFSDAADAMAKAFWPGPLTLVLP
                     GGSGRLPDLLRGPEGGIAVRWTSHPLIAALVAHLGEPLTSTSANLPGQAPDPGAAAIV
                     RDFGPAVNDGRLLVLDGGVLGNRPPSTVVDCTTPTPRLVRPGAVSQAELRAAVGSLAP
                     "
     gene            4438..4914
                     /locus_tag="VFU75_09975"
     CDS             4438..4914
                     /locus_tag="VFU75_09975"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HEU5467209.1"
                     /translation="MTKRVLLVCTGNICRSPLAAALLTKALRDRDLNDMEVSSAGTGA
                     WDGAPASEGAYLVALERGLDLSGHRARLLTRELVEESDLILTMARHHRARVDELGGDG
                     RVFVLGEFAGRSGDAEVSDPFGGDLEVYRETCAELDALITAVVERLAREAAGGSPR"
     gene            4898..5719
                     /locus_tag="VFU75_09980"
     CDS             4898..5719
                     /locus_tag="VFU75_09980"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF020090.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEU5467210.1"
                     /translation="MAHLAEPRLLAIVGDPVSHSLSPAMHNAAMRVLGLPGSYVALKT
                     PAQELTAVLSALATVGAAGNVTVPHKEAAAGYVARKTDACIRTGACNTFWVENGTLVG
                     DNTDVVGVSRALDQLGVARGGRWLVVGTGGSARAVAAAAADRGAILHVKSRMHETARR
                     FVEWARKSLSLEASPEPDGAAEVVINATPLGLRDNDHLPLAAEQVSPGAAVLDLVYRR
                     GGTAWVRALGEGRRRISDGRTTLVAQGAAAFERFFPGVHAPVEVMRAAVERALRA"
BASE COUNT          860 a         1998 c         2024 g          951 t
ORIGIN      
        1 tgctgacgaa aattctcgcg gcgattcagc tgccacgaga gcaggtctac atctgcaata
       61 tcgtgaagtg ccgtcccccg gagaatcgtg tgccgcaatt cgacgagatc tcgtcctgcc
      121 tcccgtacct ctggaggcag atcgagctgg tgcgacccaa agtcattctg gcaatgggcg
      181 ggaccgcggc gcagagtttg ctcgacgcca agcaggcact aggcgcgatg cgaaatcgag
      241 tgcatcgttt ccggggaatt cccgtggtgg tcacctacca ccccgccgcc ctgctgcgaa
      301 acccgaattg gaagaaaccg acctgggatg acgtccgctt cgcccgccgt ctcctcgacg
      361 cctgaccgca ccttcggcgg ccgccaaccg ccctggagca acgaagccga gcaggccgtg
      421 ctctgtgcga tgatgctgtc gcaggacgcc gcgctctcgg ccgtcgaggt gctggacgac
      481 accatgttct accgggaggc ccaccgcgtg ctgttccgcg cgatgtcgag cctcaccgag
      541 aagcgcgagg tcatcgaccc ggtcacgctg ggcgatgagc tgacgcgccg gggcgatctc
      601 gaccgcatcg gcggcatgga atacgtcggc gcgctcatcg acgtggtgcc gaccgcggcg
      661 aacgtcacct tccacgcgaa gatcgtccgc gacaaggcgg ttctccgccg cctggtggaa
      721 gccgcgacgg agatcatcca ggatgtctac gactcgggct ccgagtccgg cgaggtcctc
      781 gacaacgccg agcaccgcgt ctttcaggtg gcgcagctcc gccggtccga ggagttcagc
      841 cgcctgaagg agctggtctg gcccgcgatg gagcgcatcg aacagctcca gggcggggcg
      901 ggggccgtga ccggcgtcgc gtccgggttc ctggacctcg accggttgac cgccgggttc
      961 cagaagtccg acctcgtgat catcgcggcg cgcccatcga tgggcaagac ggcgtttgtc
     1021 ctgaacgtgg tccagcacgc ggccatcgag cacaacatcg gcgtggcggt gttctctctg
     1081 gaaatgtcga aggacgccct ggtgcagcgg ctcttgtgct cggaaggtct ggtcgacgcc
     1141 cagcgcctcc gccgcggcgc gctccgcgac gacgattatc ccaagctggc ccgggccgcg
     1201 ggcctgctcg ggaccgcgcc catctggatc gacgactccg cggcgctgac ccccctggcc
     1261 atgcgctcga aggcccgccg gctcaaggcc gaacacgaca tcgggctcgt ggtggtggac
     1321 tacctccagc tgatggcggg ccccaccaac gtcgagagcc gccagcagga gatctcctac
     1381 atctccagag ccctcaaggg cctggccaag gagctcaacg tgccggtgct ggctttgtcg
     1441 cagctttccc gcgcccccga gcagcggggc ggggaacacc gccgcccgca gctctcggat
     1501 ctccgcgaat cgggcgcgat cgagcaggac gccgacctgg tctgcttcat ctaccggcag
     1561 gagttctacg acggtcccgt ggatccgaaa tccgacgaga acatcgaggg cttggccgag
     1621 ctgatcgtcg gcaagcagcg caacggaccg accgggacgg tcaagctgta cttcaagaag
     1681 gagtacacgc ggttcgacaa ctacaccgag cggagtggcg ggcctccggg ggaacagttt
     1741 ggtggctaag ccccactcgg tgtttcgctg taccgagtgc ggcgccgagc agcccaagtg
     1801 ggcggggcgc tgtgattcgt gcagtgcgtg gaacacgctc gttgaagaac cggtcctcga
     1861 aaagcgccgt cagccatcag ccaccagccg tcagcaggcc ctggttggca gcgccccgcc
     1921 cgtccggctc ggagaggtca ccggcaattc gcgcgaacgc tggaagaccg gcatcgccga
     1981 attcgacttc gtgctcggcg gcgggatcgt gccgggatca ctggtgctgg tgggtggcga
     2041 gccggggatc ggaaagtcga ccctcctcct tcaggcagcc gcccagctcg agaagaacgg
     2101 cgtccgcacc ctgtacgtct ccggcgaaga gtctcccgat cagctccgcc tgcgcgccga
     2161 ccggctgggc gccgacgccc atgcggtgca cgcgctgggt gagacgcgac tcgagagcat
     2221 cgtggcgcac gcggcggcgc tgaacgccca ggtcgtgttc gtcgactcga ttcagaccac
     2281 ctacaccgaa gaactggaag gcgccccggg caacgtgggc caggtgcgcg agtgcgccgc
     2341 gcgcatgatg cgcttcgcga aagatgcggg ccccgcggtg gtgctggtcg ggcacgtcac
     2401 caagggcgga ggcatcgccg gtccccggac actcgagcac atcgtcgaca ccgtgctcta
     2461 cttcgaaggc gaagcgacgc tcgatcaccg gatcctgcgc gccgtcaaga accgcttcgg
     2521 gtccgtcgac gagatcggcg tcttccggat gaccgctgcg ggcctcgagg ccgtcccgga
     2581 cccagccgcg ctcttcctgg cgggcaggca gaccggggtt tcgggatccg cggtgaccgc
     2641 gctcatggaa ggcacccgtc ctctcctcgt cgaaatccag gcgctcgcct cgaaggcggg
     2701 cttcggcacg ccccagcgcg tcgccaccgg attcgatcag cgtcgcctgg ccgtactcct
     2761 ggcggtgctc gagcgccgcg ggggacttcc gttctcggac ctggatgtgt tcgtcaacgt
     2821 gaccggaggt gttcggctgc tcgagccgtc caccgatctc gcggtcaccg cggctctggt
     2881 ctcgagcatt cacgatcgcc ccgcacccgc cgacgcgctc ttcctcgggg aagtgggact
     2941 gggcggcgag atccgccccg tcgccggcat cgaacgccgg ctggccgagg ctacccgtca
     3001 gggattccgc cgggcgttcc tgtcggcccg gtcgcgcgtg cgggtcgagg gactggacgt
     3061 cgtcggattg gagcacattg gtgagctcgc gcggcgcctc gctgcctgac gtcggcgtcg
     3121 tcatcgtcgc ggcaggcgcg gggctccgtg ccggtcccgg cgaaccgaag cagttccgtc
     3181 ccatcctcgg cgtcccgatg ctcctgcggg ccctgcgccc cttcacctct caccccgagg
     3241 tttcgcaggt ggtcgcggtg ctcccgcgcg cgttcgtcga acacccgccc gactggcttg
     3301 cgaaggtcat gggggaccgg ctcatcctgg cacgcggagg cgagagccgg accgattccg
     3361 tgcgctccgg ggtcaaagca ctccgggacg actgccgcct gattctggtc cacgacgcgg
     3421 cccggccttt tccggtccgg gcgaccatcg acgcggtgat cgcgcgggcc cgaagcgggg
     3481 tcggggccgt agcggctgtg ccgctttcgg acaccgtgaa ggaagtgaag ggggatgccc
     3541 gggtcacccg gacggtgccc cggacaggac tctggcgcgc tcagacgccc caggggttcc
     3601 cgagaaccat gctggtggac gccttcaatc gtctgaagct ggctgacccc cctccgacgg
     3661 acgacggtga gctctgcgag cgtgccggct tccccgtaga cgtggtgcct gacagtccgc
     3721 ataacttcaa ggtgaccacg cccgacgact ttcggatcgc tgacgccctc gctcgtgagt
     3781 tgcgatgacc ggtgtagtgc ccttcgattc accccagagc gcctcctctt cgacgcccca
     3841 agtggcggca catctgcgcg gaggtgggtt ggtggcgtat cccaccgaga cggtctacgg
     3901 acttggctct ctcgcgcgag cggagcccgt aaacgccctg tcacggttga agggccgcga
     3961 accggggaaa ccctttctcc tgctcgtctc gaaccgccag atggccgagt cgtacggatt
     4021 ggcgttctcg gacgcggcgg atgcgatggc caaggcgttc tggccgggac cgctcacgct
     4081 ggttctgccc gggggcagtg gccggctgcc ggatctcctc cggggacccg aggggggcat
     4141 cgcggttcgc tggacctcgc atcccctgat cgccgcgctc gtggcgcatc tcggtgagcc
     4201 gttgacctcc acctccgcca acctccccgg ccaggctccg gacccgggtg cggccgcgat
     4261 cgtccgggat ttcggtcccg ccgtgaacga cggcaggctc ctcgtgctgg atgggggcgt
     4321 cctcgggaac cgcccgccct ccacggtggt tgactgcacg accccgactc cccggctggt
     4381 ccgtcccggt gccgtgtccc aggcggaact gcgggccgcg gtcggatcac tcgccccatg
     4441 accaagcgcg tccttcttgt gtgcaccggc aacatctgcc gcagcccgct cgcggcggcc
     4501 ctgcttacca aggccctgcg ggaccgcgac ctgaacgaca tggaggtttc gtcggcgggt
     4561 accggggcgt gggatggggc gccggcatcg gaaggcgcat acctcgtcgc cctggagcgg
     4621 ggactcgatc tctcgggtca tcgcgcccgc ctgctcactc gagagctggt cgaagagtcc
     4681 gatctcattc tcaccatggc ccggcatcac cgggcccgag tggacgagct gggtggggat
     4741 gggcgggtgt tcgtgctcgg tgaattcgcc gggcgctccg gcgacgcgga ggtgagcgat
     4801 ccttttgggg gcgacctcga ggtgtatcgc gaaacgtgcg ccgagctgga cgccttgatc
     4861 acggcggtgg tcgaacggct ggcccgggag gcggccggtg gctcacctcg ctgagccgag
     4921 gcttctggcc atcgtggggg atccggtctc ccactcgctg tcccccgcca tgcacaacgc
     4981 cgcgatgcgg gtactcggtc tcccggggag ctacgtcgcg ctcaagacgc cggcgcagga
     5041 gctgaccgcg gtgctgtcgg cgctcgcgac ggtcggcgcc gccggcaacg tcaccgttcc
     5101 tcacaaggag gcagccgcgg ggtacgtcgc gcggaaaacg gatgcgtgca tccggacggg
     5161 cgcgtgcaat acgttctggg tggagaacgg aactctggtt ggagacaaca ccgacgtggt
     5221 cggtgtctcc cgggccctgg atcagctcgg cgtcgcgcgg ggcggacggt ggctggtcgt
     5281 ggggaccggc ggctccgcgc gcgcggtcgc ggcggccgcc gccgatcggg gtgccatcct
     5341 ccacgtgaaa tcccgcatgc acgagaccgc gcgacggttc gtggaatggg cgcggaagag
     5401 tctctcactc gaggccagcc ccgagcctga cggcgcggcg gaggtggtca tcaacgcgac
     5461 tcccctgggg ctgcgcgaca acgatcacct gcctctcgcc gcggagcagg tcagcccggg
     5521 tgcggccgtg ctcgatctgg tgtaccggcg gggcgggacg gcctgggtgc gggcgctggg
     5581 agagggtagg cgccgcatca gcgacgggcg gacgacgctg gtcgcacagg gggccgccgc
     5641 tttcgagcga ttctttccgg gggtccacgc cccggtggag gtcatgcgcg ctgccgtgga
     5701 gcgcgcgctc cgtgcgtagc gcgctcgccc agctgtttct gccggccgag tgccttctct
     5761 gccacgccct tctgccgtac ctccaatccg acaggatcgt gtgccctggg tgccgtcacc
     5821 gctggcgccc cgt
//
LOCUS       DASUBP010000266         3094 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_295551, whole genome shotgun sequence.
ACCESSION   DASUBP010000266 DASUBP010000000
VERSION     DASUBP010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_295551"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..717)
                     /locus_tag="VFU75_09985"
     CDS             complement(112..717)
                     /locus_tag="VFU75_09985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467211.1"
                     /translation="MSLTQWLDQVLSPRARFLVLGAGVLLVIACAFPLWRITMFANQF
                     PEGLRLSIYSYKLVGGAGGADLEGINILNHYIGMRDIAAADFVEMRFIPFALGVFFLL
                     ALRTGVFARIRDLVDLTVLLLYFSVFSFGVFWYRMYTYGHQLNPEAPIKVAPFTPPLF
                     GHQHLANFEIYSYPGLGTFLLLGAGLVLLGVVGQSAWKAWR"
     gene            complement(719..2629)
                     /gene="nosZ"
                     /locus_tag="VFU75_09990"
     CDS             complement(719..2629)
                     /gene="nosZ"
                     /locus_tag="VFU75_09990"
                     /EC_number="1.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR04246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050304 - nitrous-oxide reductase activity
                     [Evidence IEA];
                     GO_process: GO:0019333 - denitrification pathway [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-dependent nitrous-oxide reductase"
                     /protein_id="HEU5467212.1"
                     /translation="MSRRLLSLIGLAAAAGACAPSGAPKAGQNASLADARVAAQRTYV
                     APGQLDDYYLFYSGGHSGNVFVAAIPSMRHIMTIPVFSPYPGTGYGFDEESKRMLGAY
                     TWGDVHHPALSRTKGDYDGRWLFVNDNANNRLARIDLKDFKTKQIIGPIPNSSGNHGS
                     AFLTENSEYALVASRFSVPLPMGTYAPLADYATKYHGMVSGIKIDSATGTMSLAWQIM
                     TPPFDWDLASTGKDSSADWAVWTSYNTERATGRLEVTASQRDRDYMVFVNWRQAAAAA
                     TAGKGQMMGGTRVLSPKDVAGGIYLVPCAKSPHGADFSPDGRYVVCNGKLAPYVTVFD
                     FQKFKQAVAKNDTVGNEDGIPVLRFESIRVAEVPVGLGPLHTQFDDQGYAYTSLYVES
                     AVAKWKLGPFDGHDSTLVVAKTPVHYAVGHLVVAGGDTRHPYGKYLVSMNKMSKGQHL
                     NIGPSEPESSELIDITGSGMTMLSESFTEPEPHFAQIAPAALLHPIEVYPKAENHDPN
                     AIWAASDAKITRRGNVVEVKLFAIRSYFMPNAIEVNQGDSVIIHVTNAEQERDMIHGF
                     GIALYNLNIVMDPGETKTVAFRATKPGVYSYYCTNFCSAMHQEMQGYLLVKPRGGATT
                     AAAAASGTPGGR"
     gene            complement(2649..>3094)
                     /locus_tag="VFU75_09995"
     CDS             complement(2649..>3094)
                     /locus_tag="VFU75_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467213.1"
                     /translation="RRRKEFLVNRLCSTILLFVAGTLTACGSGASKPPVMPTLPGMAS
                     SAPPTITGNDPRAYLFIRKGCPTCHSISALGVKSGTDVGPDLTYAYTDVVSRFDEPLE
                     QFLPNPTGTMQMVLSTLIKLEPAERDSVIHILKRLHDDSSASARH"
BASE COUNT          556 a          966 c         1045 g          527 t
ORIGIN      
        1 ccccgaggat cacggtgggc cgtgacacca cgaggggccc ggcgtgcagc ccgggggcga
       61 gccgcagggt atcctgtgct ccccccatcg tgagagcgag acaaagcaga atcaccgcca
      121 cgccttccag gcgctctggc ccaccacgcc cagcaacacc agtccggcac cgagcaggag
      181 gaacgtgccc aggccgggat agctgtagat ctcgaaattg gcgaggtgct gatgtccgaa
      241 gagcggcggc gtgaacggcg ccaccttgat gggcgcctcc gggttcagct ggtgcccgta
      301 ggtgtacatg cggtaccaga agacgccgaa cgagaagacc gagaagtaga gcaagaggac
      361 ggtgaggtcc accaggtccc gaatccgggc aaacaccccg gtgcgcaggg ccagaagaaa
      421 gaagaccccc agggcgaacg gaatgaagcg catctccacg aagtccgccg cggcgatatc
      481 cctcatgccg atgtagtgat tcaggatgtt gatgccttcg aggtcggcgc cgccggcgcc
      541 ccccacgagc ttgtagctgt agatcgagag ccggaggccc tcggggaact ggttcgcgaa
      601 catcgtgatg cgccagagcg gaaacgcgca ggcgatcacg agcaggactc ccgccccgag
      661 cacgaggaac cgtgcccggg gggaaaggac ctgatcgagc cattgggtca gggacatgtt
      721 accggccgcc cggcgtccca ctggcggctg cagcggccgt cgtggcgcca ccccgtggct
      781 tcaccaggag atacccctgc atttcctggt gcatcgcgga gcagaagttg gtgcagtagt
      841 acgagtacac gccgggcttc gtggcccgga aggcgaccgt cttcgtctcc cccggatcca
      901 tcacgatgtt gaggttgtac agggcgatcc cgaagccgtg gatcatgtcc cgttcctgct
      961 cggcattggt gacgtggatg atcaccgagt caccctgatt cacttcaatc gcgttcggca
     1021 tgaagtacga ccggatggcg aagagcttca cctccaccac gttgccgcgg cgggtgatct
     1081 tcgcgtccga cgccgcccag atggcgttgg ggtcgtgatt ctccgccttg ggatagacct
     1141 ctatcggatg caagagggcc gccggagcga tctgggcaaa gtgcggctcg ggctcagtga
     1201 aggactcgga gagcatggtc atcccggatc ccgtgatgtc gatcagctcg ctcgactcgg
     1261 gttccgacgg cccgatgttc agatgctggc ccttcgacat cttgttcatg gacaccaggt
     1321 acttgccata agggtgccgc gtatctccac ccgccacgac caggtgcccc acggcgtagt
     1381 gcacgggtgt cttcgcgacc acgagcgtgc tgtcatgccc gtcgaacggt cccagtttcc
     1441 acttcgcgac cgccgattcc acgtacaacg aagtgtaggc gtaaccctga tcgtcgaact
     1501 gggtatggag gggacccagg cccacgggca cttcggcgac tcgaatcgac tcgaaacgca
     1561 ggacgggaat cccgtcctcg ttgccgacgg tgtcgttctt cgcgaccgcc tgcttgaact
     1621 tctggaagtc gaagacggtc acgtagggcg ccagcttgcc gttgcagacg acgtagcgcc
     1681 cgtccggcga gaaatcggct ccgtgggggc tcttcgcgca ggggacgaga tagatcccgc
     1741 ccgcgacgtc cttcgggctg agcacgcggg tgccacccat catctggccc ttgccggccg
     1801 tcgcggcggc ggcggcctgc ctccagttca cgaacaccat gtagtcgcgg tcccgctggc
     1861 tggcggtgac ctcgaggcgg cccgtggccc gctcggtgtt gtacgaggtc cagacagccc
     1921 agtccgccga gctgtccttc ccggtggagg ccaggtccca gtcgaaggga ggcgtcatga
     1981 tctgccacgc gaggctcatg gtgccggtgg cgctgtcgat cttgataccg ctcaccatgc
     2041 cgtggtactt ggtcgcgtag tccgcgaggg gagcgtaggt gcccatcggc agcggcacgg
     2101 agaagcggct cgccaccagc gcgtactcgg agttctcagt gagaaaggcg gagccgtggt
     2161 tgccgctcga gttcgggatg ggcccgatga tctgcttggt cttgaagtcc ttgaggtcga
     2221 tgcgggcgag acgattattc gcgttgtcgt tcacgaacag ccagcggccg tcatagtctc
     2281 ccttggtacg ggacaacgcc gggtgatgca cgtcgcccca ggtgtaggcg cccagcatcc
     2341 tcttcgactc ctcgtcgaag ccatagccgg tccccggata gggcgagaac acgggaatcg
     2401 tcatgatgtg ccgcatcgac gggatcgccg ccacgaacac gttccccgag tgtccgccgg
     2461 aatagaagag gtagtagtcg tcgagctggc ccggagcgac gtacgtccgc tgcgccgcca
     2521 ctcgtgcgtc ggcgaggctc gcgttttgtc cggccttcgg ggcaccggac ggcgcgcacg
     2581 cgccggccgc cgcggccaga ccgatgagtg aaagcaggcg acgggacatg gaggctcctc
     2641 cgagaggttt agtggcgcgc cgatgccgac gagtcgtcgt gcaagcgctt caggatgtga
     2701 atcaccgaat cccgctccgc cggctcgagc ttgatgagcg tgctcagcac catctgcatg
     2761 gtgccggtcg ggttggggag gaattgttcg agcggctcat cgaagcggct gacgacgtcc
     2821 gtgtacgcat acgtcaggtc cggaccgacg tcggtcccgg acttcacgcc gagcgcggaa
     2881 atcgaatggc aggtcgggca gcccttccga atgaacaggt acgcccgcgg atcgttgccg
     2941 gtgatggtcg ggggcgcgga gctggccatc cccgggaggg tcggcatgac gggcggcttc
     3001 gatgcccccg agccgcaagc ggtcagggtt ccggccacga agagcaggat cgtggagcac
     3061 aggcggttca cgaggaactc cttccggcga cgta
//
LOCUS       DASUBP010000267         3548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_568304, whole genome shotgun sequence.
ACCESSION   DASUBP010000267 DASUBP010000000
VERSION     DASUBP010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_568304"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2670
                     /gene="uvrA"
                     /locus_tag="VFU75_10000"
     CDS             <1..2670
                     /gene="uvrA"
                     /locus_tag="VFU75_10000"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEU5467214.1"
                     /translation="YARQFLGLMEKPDVDAIEGLSPAISIEQKTAGANPRSTVGTITE
                     VYDYLRLLWARTGTPHCPKDGTPVERQSASQITDLVMQWPEGAKIEVLAPLVRGRKGE
                     FRDLFEDARKKGFVRARVDGETYDLNQVPKLNRRQNHDIAVVVDRLAVKAADRSRLAD
                     SIETALRAADGVVEVARHGSGAPKSTLFSERYACPTCGLSIPELEPRQFSFNSPFGAC
                     PTCSGLGTKREVNEDLVVGDDSLSILEGVILPWGEPSGYIRKVILPTLAKSFKFSLDA
                     PWSDLPQAARKAILHGVPGKTFTYQWDSARFHGSYESGWSGVLKHVERRYQETQSDST
                     REQLEAFMVEQPCPTCHGRRLRPESLSVLVAGRSIGDVAEMSVEDALEFFSGLQLRDK
                     GRPGLDPEIAKPILKEVKDRLRFLADVGLAYLTLGRSAGTLSGGESQRIRLATQIGSR
                     LVGVLYILDEPSIGLHQRDNAKLLTTLKELRDLGNTVLVVEHDEETIRAADYVIDLGP
                     RAGRHGGEVVAAGTLQQVLKSRESLTARYLRGELRIPVPPRRRLAGEDKHIRVVGAKH
                     HNLKDLTVDFPLGLFVAVTGVSGSGKSTLVTDILYQGLARHFYRAKVVPGVHQRLEGL
                     DQIDKVIDIDQSPIGRTPRSNPATYTGLFTPIRELYTMLPESKVRGYGPGRFSFNVKG
                     GRCEACQGDGLVKVEMHFLPDVYVPCEVCKGRRYNRETLEVRYKGKSIADVLDLSVAD
                     ALEFFDAQPRLKAKLELLNDVGLGYIHLGQSATTLSGGEAQRVKLATELAKRDTGRTL
                     YILDEPTTGLHFEDVRLLLDVLHRLVDKGNTVVVIEHNLDVVKTADWVIDLGPEGGDA
                     GGQVVAAGTPEQVARAKGSHTGRFLARVLPPGD"
     gene            2700..2975
                     /locus_tag="VFU75_10005"
     CDS             2700..2975
                     /locus_tag="VFU75_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467215.1"
                     /translation="MEDILAIVFLFGGGTIAAIAFSPIGRAIADRIRGKHSNADGIAT
                     ELSEHREETTAQITNLQEQVGELAERLDFAERMLSKQKEAERLAPPR"
     gene            2977..3252
                     /locus_tag="VFU75_10010"
     CDS             2977..3252
                     /locus_tag="VFU75_10010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467216.1"
                     /translation="MTPEEGIKFLFLFLMLGGAVWVLRPIGKAIARKLEGQAGADARA
                     LEQMRDELLGEVDGLRQDVAQLSERLDFTERMLAKQTEQPRVGPGAR"
     gene            3249..3512
                     /locus_tag="VFU75_10015"
     CDS             3249..3512
                     /locus_tag="VFU75_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467217.1"
                     /translation="MRPEEVVALMAVLACVFLVTRPLVSAFARRISRDPGTPAVTDGA
                     VDELRADLEEAHRRIAGLEERVDFAERMLAKQREPERLVPPRN"
BASE COUNT          626 a         1109 c         1226 g          587 t
ORIGIN      
        1 tatgcgcgcc aattcctcgg gctcatggaa aagccggacg tcgatgccat cgaagggctg
       61 tctccggcga tttcgatcga gcagaagacc gccggtgcca atccgcggtc gacggtgggc
      121 accatcaccg aagtgtacga ctacctgcgg cttctctggg cccgcacggg tacaccgcac
      181 tgtcccaagg acggcacgcc ggtcgagcgg cagagcgcat cgcagatcac cgatctcgtg
      241 atgcagtggc ccgagggggc gaagatcgaa gtgctcgccc cgctcgttcg cggccgcaag
      301 ggagagtttc gcgatctctt cgaagacgcc aggaagaagg gattcgtccg ggcgcgcgtg
      361 gatggcgaga cctacgacct gaaccaggtc cccaagctca atcgccgcca gaatcacgac
      421 atcgcggtcg tcgtcgaccg gctcgcggtg aaggcggcgg accgcagccg tctcgccgac
      481 tcgatcgaaa cggcgttacg cgcggcggac ggggtagtcg aggtggcccg ccacgggagt
      541 ggcgcgccca agagcaccct gttctccgag cgctacgcgt gtcccacgtg cgggctctcg
      601 attccggagc tcgagccccg gcagttctcg ttcaactcac cctttggtgc ctgtccgacc
      661 tgctcggggc tcggcaccaa gcgtgaagtg aacgaggacc tggtggtggg ggacgactca
      721 ctctcgattc tcgagggggt cattctcccc tggggtgagc cgtccggata tataaggaaa
      781 gtgatccttc cgacgttggc gaagtcgttc aagttcagtc tcgatgctcc gtggagcgat
      841 ctcccccagg ccgcgcgcaa ggcgattctc cacggcgttc ccggaaagac gttcacctat
      901 cagtgggact cggcccgctt ccacggttcg tacgagtcgg ggtggagcgg cgtgctgaag
      961 cacgtcgagc ggcggtatca ggagacgcag tcggactcga cccgcgagca gctcgaagca
     1021 ttcatggtcg aacagccctg tcccacttgc cacggacggc ggctgcgccc cgaaagtctc
     1081 tcggtgctcg tggcggggag gagcatcggg gatgtcgcgg agatgtcggt cgaggacgcg
     1141 ctcgagttct tcagcgggct tcagctgcgg gacaagggcc ggcctggtct cgaccccgag
     1201 atcgccaagc ccattctcaa ggaagtcaaa gaccggctcc gcttcctcgc cgacgtcggt
     1261 ctggcctacc tgacgctcgg gcgatccgcg ggcacgctgt cgggtggcga gtcccagcgc
     1321 attcgcctgg cgacgcagat cggctcccgg ctcgtgggcg tgctctacat cctggacgag
     1381 ccgtcgatcg gactgcacca gcgggacaac gccaagctcc tcaccacgct gaaggagctg
     1441 cgagacctgg gcaataccgt gctggtggtg gagcacgacg aagagaccat ccgcgccgcg
     1501 gactacgtga tcgatctcgg cccgcgagcc ggccgtcacg gcggcgaagt cgtcgcggcg
     1561 ggcacgctcc aacaggtgtt gaagagcagg gaatcgctga cggcgcggta tctccgggga
     1621 gagctgcgaa tcccggtccc tccgcgacgc cgcctggccg gtgaggacaa gcacatccgg
     1681 gtggtggggg cgaaacacca caacctgaaa gacctcacgg tcgacttccc cctgggcctc
     1741 ttcgtcgccg tcaccggagt ctcaggctcc ggaaagtcga ccctcgtcac cgacatcctc
     1801 taccagggac tcgcgcgcca cttctatcgg gcgaaggtcg ttcctggcgt ccaccagcgg
     1861 ctcgaggggc tcgaccagat cgacaaggtg atcgacatcg atcagagccc tatcggccgg
     1921 acgccgcgct ccaacccggc gacctacacc ggactcttca cgcccatccg ggagctctac
     1981 accatgcttc ccgagtcgaa ggtgcgcggg tatgggccgg ggcggttctc gttcaacgtg
     2041 aagggtggcc ggtgcgaggc ctgtcagggc gacgggttgg tgaaggtgga aatgcacttt
     2101 ctgcccgacg tctatgtgcc ctgcgaggtc tgcaaggggc gccggtacaa ccgggagacg
     2161 ctggaggtca gatataaagg aaagagcatc gcggacgtgc tggatctctc cgtcgccgat
     2221 gcgttggaat tcttcgacgc ccagcctcgc ctcaaggcga aactggagct tttgaacgac
     2281 gtagggctcg ggtacatcca cctcgggcag tcggcgacca ccctgtcggg cggtgaagcg
     2341 cagcgggtga aactcgccac cgagctcgcg aagcgcgaca ccggccgtac cctgtacatc
     2401 ctggatgagc ccactaccgg actgcacttc gaggacgtcc ggttgctctt ggacgtgctc
     2461 caccggctgg tggacaaggg caatacggtc gtggtgatcg agcacaatct ggacgtggtg
     2521 aagaccgcgg actgggtgat cgatctcggt cccgagggcg gtgacgccgg gggtcaggtg
     2581 gtggcggcag gcacgccgga gcaggtggcg cgggccaagg ggtcccacac gggacgcttc
     2641 ctggcgcggg tgttgccccc gggcgactga cgtcgcaggt tgactgaagg aggggcgacg
     2701 tggaagacat cctggcgatc gtgttcctct tcggtggcgg caccatcgcg gcgatagcct
     2761 tctcgcccat cggccgcgcg attgccgacc ggatccgggg caagcactcc aacgccgacg
     2821 gtatagcgac ggagctgtcg gagcaccgcg aggaaacgac ggcgcagatc acgaatctcc
     2881 aggaacaggt gggcgagctt gccgagcggc tcgacttcgc ggagcggatg ctgtccaagc
     2941 agaaggaagc tgagcgcctg gcgccaccga ggtgacatga cgcccgaaga aggcatcaaa
     3001 ttcctctttc tgttcttgat gttgggcggc gccgtgtggg tgctgcggcc catcgggaag
     3061 gcgatcgccc ggaaactgga agggcaggcc ggtgcggatg cccgggcgct cgagcagatg
     3121 cgggacgagc ttctcggcga ggtggacgga ctgcggcagg acgtcgcgca gttgtccgag
     3181 cggctcgact tcacggagcg gatgctggcc aagcagaccg aacagccgcg cgtcggcccg
     3241 ggagcgagat gagaccggaa gaagtcgtcg ccctcatggc ggtgcttgcg tgcgtatttc
     3301 tcgtcacgcg accgctggtt tccgcgttcg cccgtcgtat cagccgggac cccgggactc
     3361 ccgcggtgac ggatggcgct gtggacgagc ttcgcgccga tctcgaggaa gctcaccggc
     3421 gcatcgccgg gctcgaggaa cgggtggact tcgcggaacg gatgctggcc aagcagcgcg
     3481 aaccggagcg gctcgtgccg ccgaggaact gacgtggggt ttcttgcctt gctgatcccg
     3541 atcgtgtc
//
LOCUS       DASUBP010000268         2260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_964079, whole genome shotgun sequence.
ACCESSION   DASUBP010000268 DASUBP010000000
VERSION     DASUBP010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_964079"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1124)
                     /locus_tag="VFU75_10020"
     CDS             complement(<1..1124)
                     /locus_tag="VFU75_10020"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5467218.1"
                     /translation="MTVREARPSPTPSNPGPNPPARPPELREDLGVLIVRDAAHEVTS
                     SWRDQYGEATDPRDIVDVLQEVSEAVSARLSGTSRPLSGTSVSTLGRRLVDLLRTTII
                     RRAADPAVIPHEQLIRVLTAIEAVREQVEPDWARYFTSRLAGPEGHELLVEVAHDLRS
                     PLTSILFLAETLQRGQSGDVNDLQRRQLGLIYGAALGLSSLASDLIELVRGGDRLLDQ
                     EEAPFSITEILESVRDIVRPIAEEKRLKVRILPPPTDHRRGHHLALSRVLLNLTSNAL
                     KFTEEGFVELVCRATDRYRVEFSVRDTGKGINPEVVNNLYQPFRRTGGRRSYYLSGTG
                     LGLAICRRIVEAMGGELKVESRPDWGTRFYFEINLPPAQQ"
     gene            complement(1121..>2260)
                     /locus_tag="VFU75_10025"
     CDS             complement(1121..>2260)
                     /locus_tag="VFU75_10025"
                     /inference="COORDINATES: protein motif:HMM:NF012385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEU5467219.1"
                     /translation="HLQILIGRAAHAVRVARESKELQTEIERSHGNSEKVTVLGISPA
                     FRRAIELARKVAPTDASVLLTGESGTGKELFAQFIHQHSRRSARSFVPINCAAMPEGL
                     LESEMFGHVKGAFTGALRDKPGLLETANGGTLFMDELLEMPKPTQAKLLRVIQDGVVR
                     RVGSEHTDAVVNVRFIAATNGDPEQCVKEGTLREDLYYRLYVVPIHIPALRDRKEDIP
                     VLANFFLQHYWARHSPGAGAPPKFSEGALRALQSHPWRGNVREMQNVVEHTVVMLEPG
                     SEIQADQIPLGGGDSAAGASSAPVPRGAEALIPAIGEHEESYHVARDRLIAEFERRYL
                     TWLVNHADGNMSRAARIAGVDRTTLYRLMERHGLQRGSIASSGDA"
BASE COUNT          362 a          768 c          771 g          359 t
ORIGIN      
        1 aactgttgcg cgggcggcag gttgatctcg aagtagaagc gcgtccccca gtcgggccgg
       61 gactccacct tcagctctcc ccccattgcc tcgacgatcc ggcggcaaat ggcgagcccc
      121 agaccagtcc ctgagagata gtacgagcgc cttccgcccg tccgccggaa gggctggtag
      181 aggttattca cgacctcggg attgatcccc ttgccggtgt cccggaccga aaactccacc
      241 cggtaccggt cggtcgcgcg gcagaccagc tccacgaacc cctcttccgt gaacttcagc
      301 gcgttcgacg tcaggttcaa cagcacgcgc gagagcgcga gatgatgacc gcgccggtgg
      361 tcggtggggg gcggcaggat ccgcaccttc agccgctttt cctcggcgat cggccgcacg
      421 atgtcccgca ccgactcgag gatctccgta atggagaagg gcgcttcttc ctgatcgagg
      481 agacggtcgc cgccgcgcac cagctcgatc aagtcggacg caagagacga gagccccagg
      541 gccgccccgt aaatgagacc cagctggcgg cgctgcaagt cattcacgtc gccgctctgt
      601 cccctctgga gggtctccgc caaaaagaga atggaagtca ggggcgagcg cagatcgtgg
      661 gcgacttcca ccagcagctc gtgcccctcg ggccccgcaa ggcgcgaggt gaagtaccgg
      721 gcccagtcgg gctctacctg ctcccgaacc gcctcgatgg cggtgaggac ccgaatcaac
      781 tgctcgtggg gaatgaccgc gggatcggcc gcccgcctga tgatcgtggt ccggagcaag
      841 tccacgagcc ggcgcccgag cgtcgacacc gacgttccgg acaggggcct gctggtcccg
      901 ctgagccgcg ccgaaaccgc ctcgctgact tcctggagga cgtccacgat gtcgcgcgga
      961 tcggtggcct cgccgtactg atcgcgccag gacgacgtga cttcatgtgc ggcgtcccga
     1021 acgatcagga cgcccaggtc ttcccgcagc tcggggggcc gggccggagg attcggcccc
     1081 gggttcgagg gggtcggaga ggggcgtgct tcgcgaacgg tcacgcgtcg cccgatgaag
     1141 cgatggagcc gcgttgcagg ccgtggcgtt ccatcagccg gtagagcgtg gtgcgatcca
     1201 cccccgcgat gcgggcggcg cgggacatgt tcccgtcggc gtggttcacg agccaggtga
     1261 ggtaccggcg ctcgaactcc gcgatcaagc ggtcgcgggc cacgtgataa ctctcctcat
     1321 gctccccgat cgcggggatc agggcctccg cgcctcgcgg cacgggcgcg gacgacgccc
     1381 ccgcggcgga gtctccgccg cccagcggaa tctgatcggc ctgaatttcc gagcccggct
     1441 cgagcatcac gacggtgtgc tcgaccacgt tctgcatctc gcgcacgttc ccgcgccacg
     1501 ggtgcgactg cagggcgcgc agcgccccct ccgagaactt cggaggcgcg ccggcgccgg
     1561 gcgaatgccg ggcccagtag tgctgcagga agaagttcgc gagcaccgga atgtcttcct
     1621 tccggtcccg gagggccggg atgtgaatcg ggacgacgta gagccggtag tagagatcct
     1681 cgcggagcgt cccctccttc acgcattgct cggggtcacc attcgtcgcc gcgatgaagc
     1741 gcacgttcac caccgcatcc gtgtgctccg agcccacgcg ccgcaccacg ccgtcctgaa
     1801 tgacgcggag gagcttggcc tgcgtgggct tcggcatctc gaggagctcg tccatgaaca
     1861 gcgtgccgcc attcgcggtc tcgaggaggc ccggcttgtc gcggagcgcg ccggtgaagg
     1921 cgcccttcac atggccgaac atctcggact cgaggagtcc ctcgggcatc gccgcgcaat
     1981 tgatcggcac gaaggagcgc gccgagcggc ggctgtgctg atggatgaac tgggcgaaga
     2041 gctctttgcc cgtcccgctc tcgccggtaa ggagcaccga cgcgtcggtg ggcgcgactt
     2101 tgcgcgccag ctcgatggcc cggcggaacg cgggcgagat cccgagcacg gtgactttct
     2161 cgctgttgcc gtgcgagcgc tcgatctcgg tctgcagctc cttcgattcg cgcgcgaccc
     2221 ggacggcgtg ggccgcccgg ccgatcagga tctggaggtg
//
LOCUS       DASUBP010000269         2599 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1059360, whole genome shotgun sequence.
ACCESSION   DASUBP010000269 DASUBP010000000
VERSION     DASUBP010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2599)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2599)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2599
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059360"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1151)
                     /locus_tag="VFU75_10030"
     CDS             complement(<1..1151)
                     /locus_tag="VFU75_10030"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5467220.1"
                     /translation="MKIPFSYIWRSLWARRLTTTLTIGGIALVVFVFAGVLMLSSGIT
                     ATLVDTGSPDNAIILRKSSTSELVSQVDRGTAEILETAPEIATGPDGKPLITREVNLI
                     INLNKRNNHAALGNVTVRGTSPQSLLLRPAVKITQGRMFNFGSHELIVGKNIAERFEG
                     VTMGADVLFGGDHWTVVGYFDAGGSGFDSEIWTDADQVMQAFNRPVYSSITFRLKDAS
                     QFDALKARLAADPRTQYIEPKRERDFYREQSANMAGLIKGLGLIITIIFSVGAMIGAM
                     ITMYAAVANRTVEIGTLRALGFKRRSVLAAFLMESILLAVVGGAIGVVLASGLSWTRV
                     STVNFASFSEIGFGFALTIWIVIGAFAFALSMGVLGGFLPAVRASRLQIV"
     gene            complement(1148..2314)
                     /locus_tag="VFU75_10035"
     CDS             complement(1148..2314)
                     /locus_tag="VFU75_10035"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5467221.1"
                     /translation="MKMLSIIFRNAFRHKLRSFLTILGIATAVMAFGLLRTVVSAWNN
                     GVANSAENRMIVRQRVSFIFPLPLTDRPEIQKVPGVVEATWANWFQGVYGDANDFRNF
                     WPRFAVDPDTWFDVYPEYLVPPDQMKAFMQDRSGVILGRKTAANHNFKIGDVITLNGD
                     IFPGTWQFTVRGIYAGKEASTDETAMFLNWNYLYEQVKAREPGREVGAGWYVLKVDRP
                     DDMVKVASAVDTLFANSRAPTKTESERAFQQGFVSMSSAIITSLQVVSFVIIGIILLV
                     LANTIVMAVRERTREYAVLKTLGFTGRHVMMFILGESMLIGFFGGIVGVLLTFPMVFA
                     FGKAFSTFFPVIGVSAATAGLALMAAALCGLAAGAAPASRAARMPIVSGLRTVG"
     gene            complement(2311..>2599)
                     /locus_tag="VFU75_10040"
     CDS             complement(2311..>2599)
                     /locus_tag="VFU75_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEU5467222.1"
                     /translation="LALVGLADRMDHSPRQLSGGQEQRVAIARAIATDPQILVADEPT
                     GDLDRKSAEEILTLLERLNQEHKKTIVMVTHDPHAAERAHTVRHLDKGELQ"
BASE COUNT          500 a          832 c          829 g          438 t
ORIGIN      
        1 acgatctgca gccgggacgc ccgcaccgcc ggcaggaagc cgccgagtac gcccatcgag
       61 agcgcaaagg caaaggcgcc gatgacgatc caaatcgtca gcgcgaagcc aaatccgatc
      121 tccgagaacg acgcgaagtt cacggtggac acgcgagtcc aggacagccc cgacgccagc
      181 actacgccga tcgcgccacc gacaaccgcc agcagaatgg actccatcag aaacgcggcg
      241 agcacgctgc gccgcttgaa gcccagggcc cgcaatgtgc cgatctcgac cgtgcgattg
      301 gcgacggcgg cgtacatcgt gatcatcgca ccgatcatcg cacccaccga gaagatgatg
      361 gtgatgatga gaccaagacc cttgatgagg ccggccatgt tcgcggactg ctcgcggtag
      421 aaatcccgct cccgcttggg ttcgatgtac tgggtgcgcg ggtcggcggc gagccgggcc
      481 ttcagggcgt cgaactggct ggcatccttg agccggaagg tgatcgacga atacacgggt
      541 cggttaaagg cctgcatcac ctgatcggcg tcggtccaga tctcggaatc gaaacccgac
      601 ccgccggcat cgaagtaccc gacgaccgtc cagtgatccc cgccaaagag cacgtccgcg
      661 cccatcgtca cgccttcgaa gcgctcggcg atattctttc cgacgatgag ctcgtgactc
      721 ccgaagttga acatccggcc ctgggtgatc ttcacggcgg gccggagcag caggctctgc
      781 ggggacgtac cacggacggt cacatttccg agggccgcgt ggttgttcct cttgttcaag
      841 ttgatgatga ggttgacttc ccgcgtgatc agcggcttgc catcgggccc ggtcgcgatc
      901 tccggggccg tttcgaggat ctccgcggtg ccccggtcca cctgactcac gagctccgac
      961 gtggaggatt tcctcagaat gatcgcgttg tcgggcgatc cggtatccac cagcgtggct
     1021 gtgatgccgc tcgagagcat cagcacgccg gcgaaaacga agacgaccag ggcgataccg
     1081 ccgatcgtca gcgtggtcgt gagtcgccgc gcccacaggg agcgccagat gtacgagaag
     1141 gggatcttca tccgacggtc ctgaggccag atacgatggg catccgggca gcacggctcg
     1201 cgggcgccgc cccggccgcg agaccgcaga gcgccgccgc catgagcgcg agacccgccg
     1261 tcgccgccga gactccgatc acggggaaaa aggtcgagaa ggccttgccg aaagcgaaga
     1321 ccatggggaa ggtaagcagg accccgacga tgcccccgaa aaagccgatg agcattgact
     1381 cgccgagaat gaacatcatc acgtgccgcc cggtgaaccc aagggtcttg agcaccgcgt
     1441 attcccgggt gcgctcccgc accgccatga cgatcgtgtt cgccaggacg agcaggatga
     1501 ttccgatgat gacgaaggac accacctgca gagacgtgat gatggcggag gacatcgaga
     1561 cgaacccctg ctggaacgcg cgttcgctct cggtcttggt gggtgcccgt gagttcgcaa
     1621 agagggtgtc cacggcgctg gcgaccttca ccatgtcatc gggacggtcg actttgagga
     1681 cgtaccaccc ggcaccgact tcgcgccccg gctcccgcgc cttcacctgc tcgtagagat
     1741 agttccagtt gaggaacatc gcggtttcgt cggtcgaagc ctccttgccc gcatagatcc
     1801 cccggaccgt gaactgccag gtgcccggga agatgtctcc gttcaaggtg atgacgtcac
     1861 cgatcttgaa gttgtggttc gcggccgtct tgcgccccag aatgacgccg gagcggtcct
     1921 gcatgaacgc cttcatctgg tcagggggca ccaggtattc cgggtagacg tcgaaccacg
     1981 tgtccgggtc gacggcaaac cgcggccaga aattccggaa gtcgttcgcg tcgccgtaca
     2041 cgccttggaa ccagttcgcc catgtggctt cgacgactcc cggcactttc tggatctcgg
     2101 gccggtcggt cagcggcaga gggaaaatga acgacacgcg ctgccggacg atcatccggt
     2161 tctcggcgct gttcgccacg ccattgttcc acgcactcac tacggtccgg agcaagccga
     2221 acgccatcac cgcggtcgcg atgcccagaa tggtgaggaa cgaccgcaac ttgtgccgga
     2281 acgcgttccg gaaaatgatc gagagcattt tcattgcagc tcgcccttgt ccagatgccg
     2341 caccgtatgc gcccgctcgg cggcatgcgg atcgtgcgtg accatgacga tggtcttctt
     2401 gtgttcctgg ttcagccgct ccaggagcgt gaggatctcc tcggccgact tgcggtcgag
     2461 gtcgccggtg ggctcgtccg cgaccaggat ctgcgggtcg gtcgcgatcg cgcgcgcgat
     2521 ggcgacgcgc tgttcctgac cgccggagag ctggcggggc gagtgatcca tccggtccgc
     2581 gaggccgacg agcgccagc
//
LOCUS       DASUBP010000270         4878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_10384, whole genome shotgun sequence.
ACCESSION   DASUBP010000270 DASUBP010000000
VERSION     DASUBP010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4878
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10384"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..859)
                     /locus_tag="VFU75_10045"
     CDS             complement(119..859)
                     /locus_tag="VFU75_10045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467223.1"
                     /translation="MRFLPLLLAVLQHPQRPDIRTGLDTIYSGAFPAGAQYFEALAAK
                     DTLDPAPLIFQATAYIWWAEAKDSDDFEAARIDSLLDLAIARARADSNHFWLGTALGY
                     RAREKDLHGSALGAGKDAKAMRDAYAAVLATDSTCEDCYLGLGVYHYGLARAGGLARL
                     FAKIIGLGSGNADTGIVDLRRAANNGDLSKVEATWVLAAALVREAGRDKARRQALRAE
                     ARELVGGLASRYPGNPVFERFLKETPAP"
     gene            complement(856..1932)
                     /locus_tag="VFU75_10050"
     CDS             complement(856..1932)
                     /locus_tag="VFU75_10050"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HEU5467224.1"
                     /translation="MPPTDLRSQVTAALAALQNPRLGKDLIAAGMVRDLVEENGVVQF
                     TFLLGRDDPATLARDARRAVKAVPGVSDVRVTISDPGAPRASSQGPGPRPAGMENVPP
                     PPTAQEMPNMGKVLAVSSGKGGVGKSTVSANLATALAAQGFKVGLMDGDIYGPNIPRM
                     MGVFDKPPVTAGKIQPLEGHGVKLMSLGFIVDRDAPAIWRGPIIMKVIQQFLRDVDWG
                     KLDYFLVDLPPGTGDAQLSLTQSIHLHGAVIVTTPQEVAVGDSLRGAKMFERVGVRVL
                     GVIENMSYYVCPHCGEKSELFLSGGGERLAAELGVPLLGQIPLQARLPQHADAGRPIV
                     VADPESPAARALTDVARRVADALK"
     gene            2023..3360
                     /locus_tag="VFU75_10055"
     CDS             2023..3360
                     /locus_tag="VFU75_10055"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU5467225.1"
                     /translation="MRRPPSALFLALVLARPAAAQTTRDTVLANGLQVFVAENHRLPE
                     ATVVVAVRTGAFTQEAGQDGVAHMFEHLLFRAYPKGETAFNRETSFIDAGYNGETTGD
                     YVDYFLEAPANHASDAVKILARLVRNGRFSESDLKAERPIVLDELARGASDPQSLLER
                     RVSERLWGPIWNRVDVGGDSASVSSLSVATVQSQYTRYYVPNNMALIVSGDVSVRDIW
                     KTASDAFGDWARSADVPTPHIDYPELPQSAFLLLAAPVSDVTILVATQGPPDAPDDTD
                     AVPVEMMTAVLGNAASAFQSRLVGSGLFTSVRCRYLGHRHDGQIEFRGRTTVERGQKA
                     VLTLLNELNVLDAMTGLADEDLGYARRSTAVTDALAAEEPSGLAEALASWWGGPGIPA
                     YDAFDGRLAHVRLADVARVAGTYVAHRNRVIGIVAPRQFLVELQGRLAGGGQP"
     gene            3357..4685
                     /locus_tag="VFU75_10060"
     CDS             3357..4685
                     /locus_tag="VFU75_10060"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEU5467226.1"
                     /translation="MIPLLAVLVTTSLPAPSEPGDRADTAFTYMVGGVRVIQQIDTST
                     RLAAVGVYLLGGTTLLTPMTAGIENLFLDAAARGSKAYPRGMGTVALARTGTRETFAM
                     EEDWTAIWLQGFAEDIDSSWAVLADELVRPTLSDTLVALARDGLLAAARARYEDPEQR
                     IKALAESVMFAGHPYAVDPRGNPTSLSALSADDVRNFDRTELVGSRLMVVVVGNVTKE
                     HLTALVQSTLGSLPIGSYHWSLPPALPYREGKPGWTVEHRPLPTTYLLGYCTGPQPSE
                     PLYWPFRIANSLYSGWLGGSIRGEGLSYEAYAPFLDRALPVGGFAMSTSAPDKAFPLA
                     IEALNDLSELQIGSEETTDESGQRWFWYSRQVRRDYLNDLRDAQSTVLGTVEVLARAQ
                     LFFGNYRKVGALLPNSGAFNDRALANAAALCKSRIQYAYLGDTTRMRGKW"
     gene            4682..>4878
                     /locus_tag="VFU75_10065"
     CDS             4682..>4878
                     /locus_tag="VFU75_10065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467227.1"
                     /translation="MRPLIALTVSGLAVAATFRPLDNGLARTPPMGWNSWNHFACDVS
                     DRLIRETADAMVSSGMKDAGYR"
BASE COUNT          799 a         1646 c         1642 g          791 t
ORIGIN      
        1 ttgaagccgg tattgaacgc catcatcgcc acggtgggtg ccgcgaggcg caggatggtg
       61 gagcgaagat ctccggtggt gagcttcccg gcgggcccgg aaggcgctac ggcctcactc
      121 acggggccgg ggtctctttc aggaaacgtt cgaacacggg gttccccggg tacctgctcg
      181 ccagccctcc gaccagctca cgagcctcgg cgcgcaacgc ctgccgacgg gccttgtccc
      241 ggcccgcctc gcgcacgagc gccgcggcca gaacccaggt cgcttcgacc ttggagaggt
      301 ccccgttgtt cgccgcgcgt cgcaagtcga cgatgccagt gtccgcattt cccgacccca
      361 gaccgatgat cttggcgaag agccgcgcga gcccgccggc ccgtgccagt ccgtaatggt
      421 agacgccgag ccccaggtaa cagtcttcgc aggtcgagtc ggtggcgagc accgccgcat
      481 acgcgtcgcg catggccttc gcgtccttcc cggcacccag cgcactgccg tgcaggtcct
      541 tctcccgcgc ccgatagccc agcgccgtgc caagccagaa atgattcgaa tccgcccgcg
      601 ccctggcgat ggccagatcg agcagggagt cgatccgggc cgcctcgaag tcgtccgagt
      661 ccttggcctc cgcccaccag atgtaggccg tggcctggaa gatgagcggt gccgggtcga
      721 gcgtgtcctt ggccgcgaga gcttcgaagt actgagcccc ggccgggaac gcaccggagt
      781 agatggtgtc gaggccggtc cggatgtcgg ggcgctgcgg gtgctggagg accgccagga
      841 gaagtggaag gaatctcact tcagggcgtc tgccacccgg cgggcgacgt cggtcagtgc
      901 ccgggcggcg ggcgattcgg ggtccgcgac gacgatgggc cgtcccgcgt ccgcgtgctg
      961 cgggagccgg gcctgcaggg ggatctgccc cagcagcggg acgcccagct ctgcggcgag
     1021 gcgctccccg ccgccggaga gaaagagctc cgacttctcg ccgcaatgcg ggcagacgta
     1081 gtagctcatg ttctcgatca cgcccagcac ccgtacgccc acgcgctcaa acatcttggc
     1141 gcctcgcagc gagtcgccga cggcgacctc ctgcggcgtg gtgacgatca cggctccatg
     1201 caggtggatc gactgcgtga gcgagagctg cgcgtccccg gtgccggggg gcaggtcgac
     1261 gaggaaatag tcgagcttgc cccagtccac atcacgcagg aactgctgga tcaccttcat
     1321 gatgatgggg ccgcgccaga tggcgggcgc gtcgcggtcc acgataaagc cgagggacat
     1381 gagcttcaca ccgtggcctt cgaggggctg gatttttccg gcggtcacgg gcggcttgtc
     1441 gaacacgccc atcatccgcg gaatgttcgg tccgtagatg tcgccatcca tcaggccgac
     1501 cttgaacccc tgagcggcga gcgcggtggc gaggttggcc gacacggtgg acttccccac
     1561 gccgcccttc ccggacgaca ccgccaggac cttgcccata ttgggcatct cctgcgcagt
     1621 ggggggaggc gggacgttct ccatgccggc cggtcggggg cccgggccct gcgacgaagc
     1681 acgcggggcg ccgggatcgg agatggtcac ccggacatcc gagacccccg gcacggcctt
     1741 tacggcccgc cgggcgtcgc gcgcaagcgt cgccggatcg tcccgcccga gcaggaaggt
     1801 gaactgcacc acgccgtttt cctcgaccag atcgcgcacc atgccggcgg cgatcagatc
     1861 cttcccgagg cgcggattct gaagggcggc gagggcggcg gtgacctgcg acctgagatc
     1921 ggtgggcggc acggccggaa tctagcgggg gcttgggatg acgagcaatc cgcacagggg
     1981 cgtgatacat ttgcgcagtt catctccgtc tcgaggccac tgatgcgccg tccgccgtca
     2041 gcgctattcc tcgcgctcgt cctcgcccgc cccgccgccg ctcaaaccac gcgggacacc
     2101 gttctcgcca acggcctcca ggtcttcgtc gccgagaatc accgtctgcc cgaagcgacc
     2161 gtggtcgtcg cggtgcggac cggcgcgttc acgcaggaag cggggcagga cggtgtggcc
     2221 cacatgttcg agcaccttct gttccgggcc tacccgaagg gagaaaccgc attcaaccgg
     2281 gagacgagct tcatcgacgc aggctacaat ggggagacga cgggtgacta tgtcgattac
     2341 ttcctcgagg cgccggccaa ccacgccagt gacgcggtga agatcctcgc gcgcctggtt
     2401 cgcaacggcc gcttttccga gtccgacctc aaggcggaac gccccatcgt cctcgacgaa
     2461 ctggcgcggg gagcatccga tcctcagtcg ctcctcgaac ggcgcgtgag tgagcgcttg
     2521 tggggaccga tctggaaccg ggtggacgtc ggcggagata gcgccagcgt cagcagcctg
     2581 tccgtcgcga ccgtgcaaag tcagtacacg cggtactacg tgcccaacaa catggccttg
     2641 atcgtgagcg gggatgtctc cgtgcgtgac atctggaaga cggccagcga cgcgttcggg
     2701 gactgggccc gtagcgccga cgtgccgacc ccccacatcg actaccccga gctcccccag
     2761 agcgccttcc tgctcctcgc cgctcccgtt tccgatgtca ccatcctcgt cgccacccag
     2821 ggtccacccg acgctcccga tgataccgat gcggtccccg tcgaaatgat gacggcagtg
     2881 ctgggcaacg cggcgtcggc attccagagc cggctggtgg ggagtgggct cttcacatcg
     2941 gtccgatgcc gctacctggg acatcggcac gacggccaga tcgagttcag gggtcggacc
     3001 acggtggagc gcgggcagaa ggccgtactc accctgctga acgagctcaa cgtgctcgat
     3061 gccatgaccg gactggccga cgaggatctg ggctacgcgc gccgcagcac agcggtgacg
     3121 gacgcactgg ctgccgagga accgagcgga ttggcggaag cgctggcgtc gtggtgggga
     3181 ggacccggca tacccgcgta cgacgccttc gacggacggc tggcccacgt ccggctcgcc
     3241 gacgtggccc gcgtcgccgg gacctacgtg gcccaccgca atcgggtgat cggcatcgtt
     3301 gctccccgcc aattcctggt ggaactccag ggccggctcg cagggggagg gcagccatga
     3361 tccccctgct cgccgtcctg gtaacgacct ccttgcccgc tccttcggag cccggcgatc
     3421 gcgctgatac ggcattcacc tacatggtgg gcggcgtccg cgtcattcaa cagatcgata
     3481 cttctacccg gctcgccgcc gtgggcgtgt acctcctggg cggcacgacc ctgctcacgc
     3541 ccatgacggc cggcatcgag aatctgttcc tcgacgcggc cgcccggggc tcgaaagcct
     3601 atccccgtgg catggggacc gtggcgctgg cccgcactgg gaccagagag actttcgcga
     3661 tggaggagga ctggaccgcc atctggctcc aggggtttgc cgaggacatc gactcgagct
     3721 gggcggtgct ggcggatgag ctggtacgcc cgacgctcag tgacacgttg gtcgcgctcg
     3781 cgcgcgacgg actgctggcg gccgctcggg cccggtacga ggatcccgag cagcgcatca
     3841 aggcgctggc cgagtcggtg atgttcgcgg gccacccgta tgcggtagac ccccgaggga
     3901 acccgacttc cctgagcgct ctgagcgccg acgatgtgcg caacttcgat cgcaccgagc
     3961 tggtcgggtc gcgtctcatg gtcgtggtcg tcggcaatgt cacgaaggag catctgactg
     4021 cgctggtaca gtcgacgctc ggcagcttgc cgatcgggag ctaccactgg tctctgccgc
     4081 cggcattgcc gtatcgggag ggaaagcctg gctggacggt ggagcatcgc cccctgccga
     4141 cgacgtatct cctcggctac tgcacgggtc cccagcccag cgagcccttg tattggccgt
     4201 tccgcatcgc gaatagtctg tattccggat ggctgggcgg gtcgatccgg ggagagggac
     4261 tgtcgtacga ggcgtatgcc cccttcctgg accgggcgct gccggtggga gggttcgcga
     4321 tgagcacctc ggcgccggac aaggcgtttc ctcttgccat cgaagcgctc aatgacctgt
     4381 cggagctgca aatcggcagt gaggagacta cggatgagag cgggcaacgc tggttctggt
     4441 actcccgcca ggtccgtcgc gactacctca acgacctgcg tgacgctcag agcaccgtcc
     4501 tgggcacggt ggaggtcctt gcacgagccc agctgttctt cggaaactac cggaaagtcg
     4561 gcgccctgct tcccaactcg ggcgcattca acgaccgggc actggccaat gcggccgccc
     4621 tgtgcaagag ccgaatccag tacgcctatc ttggcgatac cacgagaatg cgaggaaaat
     4681 ggtgagacct ctcatcgccc tgacggtcag cggactggct gttgccgcca cgttccgtcc
     4741 gctcgacaac ggtctggccc gtacgccgcc catgggctgg aacagctgga accacttcgc
     4801 ctgcgacgtc tcggaccggc tcatccgcga aaccgcggac gccatggtct cgagcggcat
     4861 gaaagacgcg ggctaccg
//
LOCUS       DASUBP010000271         7765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_200007, whole genome shotgun sequence.
ACCESSION   DASUBP010000271 DASUBP010000000
VERSION     DASUBP010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 7765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7765
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_200007"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="VFU75_10070"
     CDS             <1..351
                     /locus_tag="VFU75_10070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467228.1"
                     /translation="VLVDQLFGPARLIPLAEDVAEARGRAELLRDSPNIPAAQQQLTK
                     LAREIVRSTEDRRLREEGELAATLAHVTRQDAWDHHSIDRLVAGFTRLDEMMRRRVEA
                     CSPEVRCWDLRKAG"
     gene            complement(450..968)
                     /locus_tag="VFU75_10075"
     CDS             complement(450..968)
                     /locus_tag="VFU75_10075"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5467229.1"
                     /translation="MRTLSTLVLAVVVASPLAAQGNPLSTAVQHMGRGHAKSLLAAAQ
                     AMPADKYNFKPTPAQMSFGEIIAHIADDNGITCGAISGKTLGGTKVSGSDSKEKLVAA
                     LEASLTFCNAALAGLSDAKLGDQVSWYGSNTTRAMAAVGLIDDWSDHYAMQAMYLRLN
                     GVLPPTAQQGGM"
     gene            1234..1575
                     /locus_tag="VFU75_10080"
     CDS             1234..1575
                     /locus_tag="VFU75_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HEU5467230.1"
                     /translation="MSSVLKTILLLICSNTFMTFAWYAHLKNLHDKPWIVAALASWGI
                     AFFEYLFQVPGNRIGYTTLSLGQLKMLQEVITLSVFVPFALLYMRQPLRLNFLWAGLC
                     MCGAVYFMFKK"
     gene            complement(1578..3923)
                     /locus_tag="VFU75_10085"
     CDS             complement(1578..3923)
                     /locus_tag="VFU75_10085"
                     /inference="COORDINATES: protein motif:HMM:NF039587.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HEU5467231.1"
                     /translation="MSYTPVVLIPLLLALTQAGDSSPALAVPRRVDAGVSIDGRLDEP
                     VWSEAVMLRGFSQYLPNDNRPADDSTTVLVWYSPTAIYFGIRAYQDSTRVRATLADRD
                     KISGDDYVQILLDTYHDRQQALVFGVNPLGVQGDGVIVDAPRQVAQTLSPTSTGAYTL
                     DLTPDYVYESKGHVTAWGYEVEVKIPFKTLRYQTADPQDWGINVIRVVQATGHTLTWT
                     RVLQTQASFLGQSGSLTGLTGLRRGIVLDMTPEVTSTVTGAAASPGWTYLGGTPRLGA
                     TARWGVTSNLTFNGTIHPDFSQVEADIPQIQLDPRFALNYPEKRPFFLDGLELFQTPL
                     QLIYTRRLVNPDGAAKLTGKVGSTTIAVLSGIDGTDGSAGGTDHPFLNALRARQDLGG
                     QNSVGLVYTDRADGAGFNRVGAFDTRVLLGGAWDLTAQGGASATRDSAAAPVRWGPVW
                     RAALTHSGRHFGLTATSRGYGEHFESTSGFIARTNYAVATAVPSYTIIGAPGSRVESF
                     NANVYLAGVWEEFRNFTAGQAPDTRQADFATGLTLRGGWQLGVAWSFESFGYPAFLYA
                     DYRIERIRGSVVDTIPFTGTPHLPNRDFAGSIQTPSFQKFSLVASLLVGRNDDNFFEW
                     ASAHLLVGNLDLIWRPTDRLRTELFYDHQQVNRASDGSTVSLVRIPRLKVEYQLARPI
                     FIRVIGQYTSNHTDALRDDSRTNGAILIRDPLTGTFTRTQPSTVNTFRLDWLFSYHPT
                     PGTVAYVGYGSSLTEPTPFQFRGLARTADGFFVKLSYLFRA"
     gene            4019..5089
                     /locus_tag="VFU75_10090"
     CDS             4019..5089
                     /locus_tag="VFU75_10090"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU5467232.1"
                     /translation="MIGTHRHWIRTWLGLWGWGGAIYTVLGLMGALQDEVSALRHGMV
                     VTWTEVLASNLAGHYGIAFFVPPLVLLVRRFPLARTHWQRNLPLILGATIALFLVMRF
                     GVEELEILVFTTNLIRPSSFVGGMYNLWVIVLAAQAVEFYRGEQEREHQAVELREQLT
                     HAQLEALRSQLHPHFLFNTLNAAVTLMHSDVPGADRMLTELAELLRTTLAYPGTQEIT
                     LGEELALVDRYLSIMRVRFRDRLTVQIDVAPEAQDALVPAFILQPLLENALEHGLAVK
                     PGPGRLEVTARHEDHHLAITVTDDGPGPAHNAAAGVGLTNTRDRLTQLYGADQSLTLT
                     PAGPRGGGRVRVAIPWRARPPS"
     gene            5086..5844
                     /locus_tag="VFU75_10095"
     CDS             5086..5844
                     /locus_tag="VFU75_10095"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HEU5467233.1"
                     /translation="MSRRRVVIADDEPLARERVRRMLEAGQAHTIVAEAGDGPAAVEA
                     ILEHRPEIVFLDIKMPGLDGLEVLAALSDGARPPAVIFVTAHDAHAVKAFDVGAVDYL
                     LKPFDAERFAQALGRAEATLLGGRATLGAEVKALLRSLTSRTPYPERFLVRAPKHLYF
                     VKAAEIEWAEGASNYVRLYIGGRAHLIRETLTGLTAKLPPERFVRVHRSVIVNLDQIA
                     RLEPAGHGEYEITLKSGKRVTSSRTHHAALRALL"
     gene            5859..6086
                     /locus_tag="VFU75_10100"
     CDS             5859..6086
                     /locus_tag="VFU75_10100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467234.1"
                     /translation="MEVWAGWVATTLTVSSYFFKRPITLRVIQATSACVWLVYGSLIH
                     SGPVLVTNVLVVVAALGSIFFMMKRPGSSPA"
     gene            complement(6083..>7765)
                     /locus_tag="VFU75_10105"
     CDS             complement(6083..>7765)
                     /locus_tag="VFU75_10105"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5467235.1"
                     /translation="LTTITHGIPLWKFDVRALFGPLLVELLGAAGVVLLAACANLGTL
                     LLARASARERELAVRLSLGASRRRLAGQMLVESTLLAGLGAGAGLLLANWALRLIAHR
                     LPEPVVDRAGGLALTGEVLGFTAAVAVVAVVLFGAVPAWRASRTNLIGPLNEGGRSFT
                     GRRTGWLDRSIVVAQVALALVLVNGAGMLVATLRSLRNVDAGFSTDHQLSVELDSRGT
                     PYESGGLAQFADGLLARAARIPGVRSAALSEAAPIFGGRSWRPDIAVEGYTPKPDESM
                     RCTFDPVSPGFFTTIGIGLRGGRDFTDADRGVAGVAIVNDAFVHQYLRDRNPLGATVR
                     TVQGTDTLVMQIVGVVGDARYADLRAPATPMAYIPLAQFERIPMLGRLAVLTLSVRTV
                     GDDRAMATSLRNAVFTEAPATQAYGPEPIEASIDASLTRETLTAEVATLFGVVALILA
                     AIGLYGVVSYRVTQRTREIGVRMALGAAAPNVVWLVFRQALALVAIGVLVGVPLTFAS
                     GKAIAAELYGLGHNPLYVLGAGVLLVGVAVAASALPARRAAAVDPLIALRAD"
BASE COUNT         1338 a         2578 c         2587 g         1262 t
ORIGIN      
        1 gtactggtgg accagctctt cggtccggcg cgcctgattc ccctggccga agacgtggcc
       61 gaggcccggg ggcgtgcgga actgcttcgt gactcaccga acatccccgc ggcgcaacag
      121 cagctcacca agctcgcccg cgaaatcgtg cggtccaccg aagatcgccg cctccgcgaa
      181 gaaggagagc tggcggcaac gcttgctcac gtcacccgcc aggacgcctg ggatcaccac
      241 tcgatcgacc gcctcgtcgc cgggttcacg cgtctcgatg aaatgatgcg ccggcgagtg
      301 gaggcgtgct ccccggaagt gcgctgctgg gacctgcgca aggccggatg aagatcactc
      361 catcagggag ggagtgatgg ccaaggcacg ggaagctgtt ccgtcggccg tcagcggtgc
      421 tgaccgctga cggctgacgg ctgcttctct tacatccccc cctgctgcgc cgtcggcggc
      481 aggacgccgt tcaaccgcaa gtacatcgcc tgcatggcgt agtgatccga ccagtcgtcg
      541 atgaggccga cggcggccat ggcgcgggtg gtgttcgagc cgtaccacga gacctggtca
      601 ccgagcttcg catccgacag gcccgccaga gccgcgttgc agaaggtgag tgaggcctcg
      661 agcgcggcca ccagcttctc cttggagtcg gatcccgaca ccttcgtgcc acccagggtc
      721 ttcccgctga tcgctccaca cgtgatgcca ttgtcatcgg cgatatgtgc gatgatttct
      781 ccgaacgaca tctgggcggg cgtgggcttg aagttgtact tgtcggccgg catggcctgc
      841 gcggcggcaa gcaagctctt ggcatgaccg cgtcccatgt gctgcaccgc ggtggacaga
      901 ggattcccct gcgcggccag gggtgaggcg acgacgacgg caagaaccag ggtgctgagg
      961 gtacgcatcg gtgctccgga gtgggtgaat gggggaagga acggccggga tgcccgcaac
     1021 acaacggggg tgtggcgaac ggggtttcgg cggggaccag tggtccgaaa atggcggtca
     1081 gcgggagaga agcaggggtc aggggtcagg ggtcaggggt cgggggtcgg gggtcagggg
     1141 tcaggggtca ggggtcaggg atgcgagaga tatgctcccg caatagcatt ggggggcttt
     1201 tccctgacct ctcacccctg acccctggac cctatgtctt cggtgctcaa gaccatcctt
     1261 cttttgatct gctccaacac tttcatgacc ttcgcgtggt acgcgcatct caagaacctg
     1321 cacgacaagc cttggatcgt cgcggcgctg gcgagctggg ggatcgcctt cttcgagtac
     1381 ctgttccagg tcccggggaa tcgcatcggc tacaccaccc tgagcctcgg gcagctcaag
     1441 atgctccagg aagtcatcac gctcagcgtg ttcgtgccct tcgccctcct ttatatgcgg
     1501 cagccgctcc ggctcaattt cctttgggcg ggtctgtgca tgtgcggggc cgtctacttc
     1561 atgttcaaga aatagcgcta cgcgcggaac aagtagctga gcttgacgaa aaatccgtcc
     1621 gcggtccgcg ccaggccccg gaactgaaaa ggcgtcggct cggtgaggct cgagccgtac
     1681 cccacgtagg ccacggttcc tggggtgggg tggtacgaga acagccagtc gagccggaac
     1741 gtgttcacgg tgctcggttg ggttcgcgtg aaggtccccg tcaaggggtc gcgaatcaga
     1801 atggcaccgt tggtccgcga gtcatcccgc agagcatccg tgtgattcga agtgtattgc
     1861 ccgatcaccc ggatgaagat cggccgtgcc agctggtatt ccaccttcag acgcgggatg
     1921 cggaccaggg acaccgtact cccgtcggac gcccggttca cttgctggtg atcgtagaac
     1981 agctcggtcc gcagacggtc cgtcggccgc cagatgagat ccaggttccc gacgaggagg
     2041 tgggcgctcg cccactcgaa aaagttgtcg tcattccgcc ccaccaggag cgacgccacg
     2101 agcgagaact tctggaaact cggcgtctga atgctcccgg cgaaatcccg gttgggcaga
     2161 tgcggcgtgc cggtgaacgg gatggtatcc acgaccgatc cgcggatgcg ctcaatgcgg
     2221 tagtcggcat agaggaacgc cggatagccg aaagactcga acgaccaggc aacgccgagc
     2281 tgccaaccgc cgcggagcgt cagtcccgtg gcgaagtcgg cctgccgagt atcgggggcc
     2341 tgtccggccg tgaaattccg gaattcctcc catacgcccg cgaggtagac gttggcgttg
     2401 aagctttcga cccgggatcc cggcgccccg atgatcgtgt agctcggaac ggcagtcgcg
     2461 acggcgtaat tggtccgggc aatgaacccg ctggtggact cgaagtgctc gccatacccg
     2521 cggctcgtcg ccgtgaggcc gaaatgccgc ccggagtgcg taagggcggc acgccagaca
     2581 gggccccagc gtacgggcgc cgcggccgaa tcacgtgtcg cgctggcgcc gccctgcgca
     2641 gtgaggtccc acgctccccc gaggagcacc cgggtgtcga aggcgccgac ccggttgaaa
     2701 ccagcaccat ccgctcgatc cgtgtaaacc agtccgacgc tgttctgtcc cccgagatcc
     2761 tgccgggcgc gaagcgcgtt caggaacgga tgatccgttc cgccggccga tccgtccgtg
     2821 ccgtctatcc cggagagcac cgcgatcgtg gtcgatccca ccttccccgt gagcttggcc
     2881 gcaccgtcag ggttcacgag gcggcgcgtg tagatgagct gcagcggtgt ttgaaagagc
     2941 tcgaggccat ccaggaagaa cgggcgcttt tcaggatagt tcagcgcgaa gcgcgggtcg
     3001 agctgaatct ggggaatgtc cgcctcgact tgcgagaaat ccggatggat ggtgccgttg
     3061 aacgtcaggt tggaggttac gccccatcgc gccgtcgctc ccagccgagg ggtcccgccc
     3121 aggtaggtcc accccgggga cgccgcagca cccgtcacgg tgctggtcac ctcgggcgtc
     3181 atgtccagca cgatgcctcg acgcagcccg gtgaggccgg tgagcgagcc gctctgaccc
     3241 aggaacgaag cctgcgtctg gagcacgcgg gtccaggtca gagtgtgccc ggtcgcctgg
     3301 accacccgga tcacgttgat gccccagtcc tgggggtccg ccgtctgata acggagggtt
     3361 ttgaagggaa tcttgacttc gacctcgtat ccccaggccg tgacgtgccc cttcgactcg
     3421 tagacataat cgggggtcag gtcgagcgtg tatgcgccgg tgctcgtcgg tgacagggtc
     3481 tgcgccacct ggcggggagc atcgacgatg actccgtcac cctgcacacc gagggggttc
     3541 acgccgaaca ccagggcctg ctgccggtcg tgataggtat cgagcagaat ctggacatag
     3601 tcgtcacccg agatcttgtc tcgatccgcg agcgtggcgc gaaccctcgt agagtcctgg
     3661 taggcccgga tgccgaaata gatcgcggtg ggagaatacc agacgagcac cgtagtcgag
     3721 tcatcggccg ggcggttgtc gttcgggagg tactgggaga agccgcggag catcacggcc
     3781 tcgctccata ccggctcgtc cagccgccca tcgatgctta cgccggcgtc gacccgtcga
     3841 ggcaccgcga gcgccgggga tgaatcgcca gcttgcgtca gggccaggag caggggaatc
     3901 agcacgacgg gagtgtagga caccgggctc ccgcccgacc aacagcaggg gacgaccctg
     3961 ccctgcggag ggaccaacgg tccgagccgg gggacgacgg atggatagtt taggggccat
     4021 gatcggcaca catcggcact ggatccggac ctggctcggc ctctggggat gggggggcgc
     4081 catctatacg gtgctgggcc tcatgggagc tctccaggac gaagtgtccg ccctccgcca
     4141 cgggatggtg gtcacctgga ccgaggtact cgcgtccaac ctcgccggcc actacggcat
     4201 cgcgttcttc gttccacccc tcgtgcttct ggtgcggcgc ttcccccttg cccggacgca
     4261 ttggcaacgc aacctgcccc tcatcctcgg tgccaccatc gccctgttcc tcgtcatgcg
     4321 cttcggggtc gaggagctcg agatcctcgt gttcaccacg aatctgattc gtccctcgag
     4381 tttcgtgggc gggatgtaca acctctgggt catcgtcctc gcggcacagg cggtcgaatt
     4441 ctatcgggga gagcaggagc gggagcatca ggcagtggag ctgcgggaac agctcaccca
     4501 cgcccagctc gaggcgctgc ggtcacagct ccacccgcac ttcctgttca acaccctcaa
     4561 cgccgccgtc accctcatgc actcggacgt cccgggggcc gaccggatgc tcacggagct
     4621 ggccgaactc ctccgcacga cgctcgcgta tccgggcacg caggagatca cactcggcga
     4681 agagctggcg ctcgtcgacc gctacctctc gatcatgagg gtccggttcc gggaccggct
     4741 caccgtccag atcgacgtcg cgccggaggc gcaggacgcg ctggtccccg ccttcattct
     4801 tcaacccctt ctcgagaacg ccctcgagca cggcctggcc gtgaagccgg gaccggggcg
     4861 actcgaagtc acggccaggc atgaggacca tcacttggcc atcacggtga ccgacgatgg
     4921 tcccggcccg gcgcacaacg cggcggcagg ggtcggcttg accaataccc gcgaccgcct
     4981 gacgcagttg tacggcgccg atcaatcgct caccctgacc cccgccgggc cccgcggtgg
     5041 tggacgagtc cgcgttgcca tcccctggcg tgcacgaccg ccgtcgtgag ccggcgacgg
     5101 gtggtcatcg ccgacgacga gccgctcgcc cgcgagcggg tgcggcggat gctcgaagcc
     5161 ggccaggctc acacgatcgt cgccgaagcg ggggatggac cggccgccgt cgaagcgatt
     5221 ctcgagcacc gacccgagat cgtcttcctc gacatcaaga tgccggggct ggacggcctc
     5281 gaagtcctgg ccgctctctc cgacggcgcg cgccctccag ccgtgatctt cgtgaccgca
     5341 cacgacgcgc acgccgtgaa ggcatttgac gtcggggccg tcgactatct cctgaaaccc
     5401 ttcgacgccg agcggttcgc ccaggcgctc ggccgtgcgg aggccacact cctgggagga
     5461 cgcgccacgc tgggagccga agtaaaggcc ctgttgcggt ctctgacctc ccgaaccccc
     5521 taccccgagc gcttcctggt gcgcgcgccg aagcacctgt atttcgtcaa ggcggccgag
     5581 atcgagtggg ccgagggggc gtcgaactac gtccggttgt acatcggagg cagggcccac
     5641 ctcatccggg aaactctcac gggtctcacc gccaaactgc ctcccgaacg cttcgtgcga
     5701 gtacaccggt ctgttatcgt caatctggac cagatcgccc ggctcgaacc ggcggggcac
     5761 ggcgagtacg agatcacgct caagagcggg aagcgggtca catcgagccg gacgcaccac
     5821 gcggcgttgc gggcacttct ctagttccgg gagacctcat ggaggtatgg gcgggctggg
     5881 tagcgacgac gctgacggtg tcctcgtact tcttcaagcg gccgatcacg ctgcgggtga
     5941 tccaggcgac gagcgcgtgc gtctggctcg tgtacggctc gttgattcat tccggtccgg
     6001 tgcttgtcac caacgtgctg gtcgtggtgg ccgccctggg gtcgatcttc ttcatgatga
     6061 agaggcccgg cagctctccc gcctagtccg cccgcaacgc gatcagcgga tccacggctg
     6121 cggcccgtcg cgcgggcagc gcgctcgcgg ccacggccac tcccaccagg agcacgcccg
     6181 cgccgaggac gtagagcgga ttgtgcccca gtccgtaaag ctccgcggcg atcgctttgc
     6241 cgctcgcgaa cgtgagtggg acgcccacca gcaccccgat cgccaccagc gcgagggcct
     6301 gccggaacac gagccacacc acgttcggtg ccgcggcccc gagggccatg cgcacgccga
     6361 tctcccgcgt gcgttgggtg acgcggtacg acacgacgcc gtagagcccg atggccgcca
     6421 gaatcagcgc cacgaccccg aacagcgtcg cgacctcggc ggtcagcgtt tcgcgagtga
     6481 gggacgcgtc gatggaggcc tcgatcggct cgggaccgta ggcctgggtc gccggtgctt
     6541 cggtgaatac cgcgttccgg agtgaagtcg ccatggcgcg gtcgtccccc acggtgcgca
     6601 ccgagagggt gagcaccgcc aggcgaccca gcatcggaat ccgttcgaat tgcgcgagag
     6661 gaatgtaggc cattggagtc gccggggccc gcaagtcggc gtagcgggcg tctcccacca
     6721 ccccgacgat ttgcatgacc agcgtgtccg taccctgcac cgtgcggacg gtcgccccca
     6781 ggggattccg atcccgcaag tactgatgga cgaacgcgtc gttgacgatc gccaccccgg
     6841 cgacaccgcg atcggcatcg gtgaagtccc gcccaccgcg caggccgatg cctatcgtgg
     6901 tgaagaaccc cggactcacc gggtcgaacg tgcatcgcat gctttcgtcg ggcttcgggg
     6961 tgtagccttc gaccgcgatg tcgggacgcc agctgcgccc accaaaaatc ggtgccgcct
     7021 ccgacagcgc cgccgaccgt acacccggga tccgcgcggc gcgagccagc agcccatcgg
     7081 caaattgcgc caggccaccg ctctcgtacg gagtgcctcg gctgtccagt tctacgctca
     7141 gctgatgatc cgtggaaaat cccgcgtcca cgttccgcag gctgcggagc gtcgcgacca
     7201 gcatgccggc gccattgacc agtaccaacg ccagcgcgac ttgcgcgacg acgatgctcc
     7261 ggtccagcca gccggtgcgc cggcccgtga acgagcgtcc gccctcgttc aggggcccaa
     7321 tcagattggt ccgtgacgcg cgccaggccg gtacggcgcc gaacagcacc accgcaacga
     7381 ccgccaccgc ggcggtgaaa ccgagcactt ctccggtcag ggcaagtccc ccggcgcggt
     7441 cgacgacggg ctcgggaagg cgatgcgcga tcaagcggag cgcccaattt gccaacagga
     7501 gtccggcccc ggcgccgagg ccagccagga gggtgctctc cacgagcatc tggcccgcca
     7561 ggcggcggcg ggaggcgccg agggagaggc ggacggccaa ctcgcgttcc cgcgccgaag
     7621 cccgcgcgag cagcagtgtg ccgaggttcg cgcacgcggc gagcagcacg accccggccg
     7681 cgcccagcaa ttcgaccagc aaggggccga acagggcgcg gacgtcgaac ttccataggg
     7741 gaatgccgtg cgtgatggtc gtgag
//
LOCUS       DASUBP010000272        19212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1059410, whole genome shotgun sequence.
ACCESSION   DASUBP010000272 DASUBP010000000
VERSION     DASUBP010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 19212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19212
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1059410"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="VFU75_10110"
     CDS             <1..426
                     /locus_tag="VFU75_10110"
                     /inference="COORDINATES: protein motif:HMM:NF014788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIR synthase-related protein"
                     /protein_id="HEU5467236.1"
                     /translation="MGLGIDDPFPETSESVAQVLLAVHRSYAAAVMPVIARIHALAHI
                     TGGGIPGNLIRVLPDGCEALVEAGAWEWPPLFRVLMRAGKVSLPEMRDVFNLGIGMIA
                     VAGRDDVEPVVAAAARAGIPAWVIGEIRPGKTGVQFSQR"
     gene            447..1361
                     /locus_tag="VFU75_10115"
     CDS             447..1361
                     /locus_tag="VFU75_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467237.1"
                     /translation="MRRIGILLGLALAAGAPLAAQCNTDCQRAEDALKSFHPVAGLIV
                     SGGNPVLGSGGTLGGLGHFFVSARVNAVKVALPSTSSTDTAATSAVVPAPVVEAGLGI
                     WNGLGGGLLSVDALASATLLPTTQVSNLKVDSSATKVGSLALGLGYGLRVGIIRGSFF
                     VPSVSVSVMRRTLPRLTYGTLGAVPSTDSAFSFDTDLKATNFRVTAGMHLLLLDVAAG
                     FGYDIYTSNAHIAYYNSPITVAHDTLNLNNKREVIFVNAGMNLMLVKVVGELGYQTGK
                     DQTLSTNYQGLDPKAGHVFGGVGVRISF"
     gene            1384..2454
                     /gene="ribD"
                     /locus_tag="VFU75_10120"
     CDS             1384..2454
                     /gene="ribD"
                     /locus_tag="VFU75_10120"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEU5467238.1"
                     /translation="MTRALDLAMRGWGRVAPNPMVGAVVLQNGKAVAEGWHAEFGGPH
                     AEVAALNAAGGQARGSTLVVSLEPCRHTGKTPPCVDAILAAGVRRVVAAIPDPDPQAG
                     GGLEKLRLAGVDVTMGIEIERAASLNAAFLHSRTDHERPFVALKLATSVDGRIADHAG
                     RSQWVSGEEARDYVHWLRAGHDAIAVGGTTALRDNPRLTVRGAVTPRRPPVRVVFDRR
                     AMLNPTVTLVQTAREIPTWVMASPDAPATNASVLDANGVRVFRPLDVQAGLRELRAAG
                     IQSVLCEGGGALGAKLLSLGLVDRLYWIQAPLWLGDDGIPAFPGVANVDIDQAPRWIP
                     VERKALGADTLLVLDRRLCLPE"
     gene            2439..3047
                     /locus_tag="VFU75_10125"
     CDS             2439..3047
                     /locus_tag="VFU75_10125"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEU5467239.1"
                     /translation="MFTGIVTAVGRVKASRREPGAGSRPSGVALTIGAPYRGLKRGES
                     IAVNGACLTVEKVVKGGFTVHAIATTLDRTLIGEYAAGRQVNLERALKASDRLGGHIV
                     QGHVDGVGRVIAVADRDDARLLDLEVPGDVREVTVPHGSITVDGVSLTVNELPPSGVV
                     QVSLIPFTRTHTTLGAARVGDRVHVEGDVLGKYVKALCRTNS"
     gene            3029..4234
                     /locus_tag="VFU75_10130"
     CDS             3029..4234
                     /locus_tag="VFU75_10130"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA];
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEU5467240.1"
                     /translation="MSHELVEQAIADIRAGKLVIVADDEDRENEGDLVGAAARVSPEM
                     INYMATHGRGLICLTLTPDRCQALGLSQMTEHNTESRETAFTVSVDAGARFGVTTGIS
                     AADRAATIRVAIDPASEPSDLRRPGHVFPLRSRPGGVLQRVGQTEASVDLARLAGLYP
                     AGVICEILNADGTMARRPELDRFSAEHKLTFITVAQLVQYRLAKERLVHRVASARLPT
                     AAGEFKIIGYRNDVDEAEHVALVYGDVGGQPAVMVRMHSKCLTGDVFGSQRCDCGWQL
                     ESAMHTIAREGRGVIVYLDQEGRGIGLLNKLKAYELQDAGNDTVEANEKLGFKPDLRN
                     YGIGAQILLDLGLSSIRVLTNNPKKLVGLEGYGLEIVERLPIVPPPSDENRAYLDVKR
                     DKLGHLLTH"
     gene            4236..4715
                     /gene="ribH"
                     /locus_tag="VFU75_10135"
     CDS             4236..4715
                     /gene="ribH"
                     /locus_tag="VFU75_10135"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF013081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEU5467241.1"
                     /translation="MTEFRGTPRAPATRRVALVVSRYHELITGKLLEGARACCREHGV
                     KDDHVDVLWTPGAFELPVVAEAAASSGRYAAIVALGCVIRGETPHFDYVAGEATRGLG
                     NVALAHQVPVGFGVLTVDTQEQAEARAGGSAGNKGFEAAEAALATADLLAQLPRAAR"
     gene            4702..5115
                     /gene="nusB"
                     /locus_tag="VFU75_10140"
     CDS             4702..5115
                     /gene="nusB"
                     /locus_tag="VFU75_10140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU5467242.1"
                     /translation="MLRAETKARARALQILYAWDLSGRPSVEAVVARLGVAFGHEPEG
                     YDRGADLAARAAAGTEDYDRRMADAAEHWRLERVGAVEKNILRIAMAELDEGVTPPRV
                     VIDEAVRLAHWFAGPKAPGFVNGVLDTVAREQGLL"
     gene            5112..6239
                     /locus_tag="VFU75_10145"
     CDS             5112..6239
                     /locus_tag="VFU75_10145"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEU5467243.1"
                     /translation="MRILLVNWNDRENPQAGGAEIHLHEIFGRLARAGHQIDLVVSGW
                     AGASAQATVDGMRVHRVGDRNTFAMLGRGAVRRRLAANTYDIVVEDINKLPLFLASAT
                     RLPFCVIVPHLFGTTVFSEASWPVAALVWAAERPIPWAYRRAAFHAISDSTKADLVAR
                     GVPAERIEVIYPGVDANWYRPDSSVRRAARPTFLYVGRLKRYKGVEYALRALAAARVQ
                     GGDLRLDIAGKGDDRPRLERLARDLGLMETVRFLGFVDDETKRRLLRESWAVVFPSPK
                     EGWGITNVEAAACGTPALASDSPGLRESVRDGETGYLVPHGDVTALADRMLVLANDPR
                     RVETLGAAGRRFAEGLTWDRAAAETGRHLERIRSSHGGENR"
     gene            6239..7207
                     /gene="hprK"
                     /locus_tag="VFU75_10150"
     CDS             6239..7207
                     /gene="hprK"
                     /locus_tag="VFU75_10150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr(Ser) kinase/phosphatase"
                     /protein_id="HEU5467244.1"
                     /translation="MANPKVRDLLARKAEPLQLEALTGELGFDREIPAPEASSPGLVL
                     AGYTARFVGNRIHVLGETEISYLSSLEPDARRGCLQNLFKFELPVVVITKGQSPPAEL
                     LELAQARGVALVRTQLKTSEFYRRLKPFLDDAFAPTTTTHASLADVFGVGLLFMGRSG
                     IGKSECVLDLVERGHRLVADDVVHVTRRGNDVLIGRGHELSRHFMEIRGVGLIDIQAL
                     FGIRAVRQQKRIEVVVQLEDWDANREYDRTGLDRQETTLLGVSLPLVTVPLNPGKNLT
                     VICEVVAMNHLLRYGGVDSAKEFNDRLMRTMRQRRDVGTYLEEDDE"
     gene            7200..7583
                     /locus_tag="VFU75_10155"
     CDS             7200..7583
                     /locus_tag="VFU75_10155"
                     /inference="COORDINATES: protein motif:HMM:NF015567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467245.1"
                     /translation="MSNSLRGVVVSHAGLAQSLVTAAETISGVAGALVPVTNEGCDGG
                     SLKARIGEAIGDGPAVVFVDLPGGSCLAGAVRIARDQGRGIAVVTGVNLAMLLDFVFH
                     RDLPPAEAAQRAVDAGGRAIRIPPA"
     gene            7580..8056
                     /locus_tag="VFU75_10160"
     CDS             7580..8056
                     /locus_tag="VFU75_10160"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEU5467246.1"
                     /translation="MSLAVVRVDDRLIHGQVVVGWGQPLGLGFIALVDDKVRSSGWEQ
                     DLYRMGVPPGIEAIFASVDEAARRYEDWVKDARIGLVLTGDIDTLIALSDKVPKLRRV
                     TLGGIHHKPGRSERLRYVYLTADEVAQLRELTKRGIEVVAQDVPTARAVPLAEFAK"
     gene            8056..8727
                     /locus_tag="VFU75_10165"
     CDS             8056..8727
                     /locus_tag="VFU75_10165"
                     /inference="COORDINATES: protein motif:HMM:NF015566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEU5467247.1"
                     /translation="MPHGWELLAFGAVVGLDLVSFPQMMLARPIVAGAGAGLLLGDVG
                     AGLRMGVLFELFQYDILPVGATRYPEYGPATVIAVGAAHAFALPFGIGLATVVGLFTA
                     MIGGISLQILRRLNTRAVTAAAPALERGDVRALYRLHASGAARDLARAVLVTAFGLVL
                     VAITREFVVPALSHRGERLLSVAATGAGLAAGAAGTLRLVGLGTHLKWLAVGLVGGIV
                     LAWLT"
     gene            8715..9461
                     /locus_tag="VFU75_10170"
     CDS             8715..9461
                     /locus_tag="VFU75_10170"
                     /inference="COORDINATES: protein motif:HMM:NF015570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEU5467248.1"
                     /translation="MADVKALPRSRLAISFLRLFTVQASWNYERLIGVGTGFAAEPLL
                     RGLDSERYRAALGRASRYFNSHPYLSGAGAGALARAEVDGVPAEQVERLRGALAGPLG
                     SIGDRLVWAGWLPMLSALALTGVTWGRGLTAVAWFLVVYNLGHVALRWWALRAGWNYG
                     PRVAAALQEPWMRRAGQYLGPATALALGLALPMVAQWSGDAFRGWPRIMLAVAAAVAF
                     GLLRWRPDILSGLRLGLVLVVIALIAGMLW"
     gene            9458..9736
                     /locus_tag="VFU75_10175"
     CDS             9458..9736
                     /locus_tag="VFU75_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017978864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HEU5467249.1"
                     /translation="MIEREALIVNPLGVHARPAAEFVKTANRFSAAVEIRRGEMSVNG
                     KSIMGVMMLAAEQGATIVISANGDDAEAAVEALCALVAAGFNEMDLTE"
     gene            9733..11532
                     /gene="ptsP"
                     /locus_tag="VFU75_10180"
     CDS             9733..11532
                     /gene="ptsP"
                     /locus_tag="VFU75_10180"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEU5467250.1"
                     /translation="MSDRTLRGIGVSPGIAIGPAVSVQTTLPEVPHRVVLRTQVEKEV
                     RRLRAAVRDVRRHLTELRKRAEVEAGADEARIFDAQILMLEDKAFIGSVEELIRKNHL
                     TAEKAYEYQALEVRDLWAASTNTRLKERLADLNGVTIQVVRHLLHKGDLVAGGIREPS
                     ILVAHELAPGVTVQFGRDRVLGLVSEEGTRTSHAAILAHSMSMPAVMGLAGALERIKT
                     GTMLIVDGTRGVVILDPSKEEIAAARAMDTRRKQIARQAEEGANLPAVTTDGIHINVR
                     GNVDLPEEIKAAKEHGAEGVGLLRTEFLITGHTKLPTEDEQAKYFRKVGDAFPGYPIV
                     IRTYDLGGDKFPAPFRAPPEANPFLGWRAIRVCLDQPEMFRVQLRAALRAAAHVPIHL
                     MIPLVTRLSEVETTRQMVAEEGQLLKRAGIQAAETVPVGVMVETPAAAVMADRLAEIS
                     DFLSVGTNDLTQYTLAVDRSNARLANRFTPHDPSVLRLLKLVAEAARAAGKPASVCGE
                     MASEPISAFLLLGLGYETLSVAPPALPLIRWLVRQVSREQAMSAAAAALAARSADLAL
                     DILRYALAEAVDMKLLDPDARLPGGRTSASLPS"
     gene            11549..12742
                     /gene="metK"
                     /locus_tag="VFU75_10185"
     CDS             11549..12742
                     /gene="metK"
                     /locus_tag="VFU75_10185"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEU5467251.1"
                     /translation="MLDARHVFTSESVTEGHPDKIADQISDAILDAILTNDSAARVAC
                     ETLVTTGMAVVAGEITTTTYVHIPDIVRGTIKAIGYTDASYGFDAETCAVLTTIDRQS
                     ADIAMGVDIGGAGDQGMMFGYATNETASLMPLPIVLAHRMAERLAAVRKDGTLPYLRP
                     DGKTQVSVVYEGSRPVGISKVVVSTQHSDRIKANELREAVIKTVVTPVLTDAGFKVAD
                     ADILVNPTGRFVVGGPQGDAGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAAYA
                     MRWVAKNLVQAGLAARCEAQVAYAIGVADPVSVMIDTFGTGQVPEAALEEAVREVFDL
                     TPAGIIKALELRKPIYKPTAAYGHFGREPGANGLFSWERTNRVEELRLAVQRSRHYRS
                     NKVTA"
     gene            12739..14088
                     /gene="ahcY"
                     /locus_tag="VFU75_10190"
     CDS             12739..14088
                     /gene="ahcY"
                     /locus_tag="VFU75_10190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HEU5467252.1"
                     /translation="MTPSTTAVSETSDIKNPALADEGRRRAEWAERSMPVLRQVRERF
                     AREKPLAGRKLSACLHVTSETANLMVTLKAGGADVALCASNPLSTQDDIAAHLVTHHG
                     IKVFAIKGEDHETYYEHLRAALAHQADVTMDDGADLVGALHMIALNRLDDLAPPVRRW
                     VEKMSAAERGALVKRVIGSTEETTTGVIRLKAMAKDGVLQFPVIAVNDSDTKHLFDNR
                     YGTGQSTLDGILRATNLLIAGSTVVVAGYGWCGRGFAMRARGAGAMVIVTEIDPLKAL
                     EAQMDGFQVAPMAEAAKVGDIFVTLTGNVHVIRLEHMKAMKDGAVIANSGHFNVEIDL
                     DALKGAATGPDHVRDLVEGWTLGGKKIFVLADGRLVNLAAAEGHPASVMDMSFANQAL
                     AAEYVVKEFKQLGKDVHRIPRDLDQDIARLKLAGMGVRIDTLTAEQQKYLASWDMGT"
     gene            14099..14620
                     /locus_tag="VFU75_10195"
     CDS             14099..14620
                     /locus_tag="VFU75_10195"
                     /inference="COORDINATES: protein motif:HMM:NF014618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HEU5467253.1"
                     /translation="MKEVRVANLGLDRTNNTPVVILRESDGERVLPIWIGPAEASAIA
                     MELAGVKSSRPLTHDLLKQVIVGLGAELRKIIITAVKDSTYYAELHLYRGDQVIQIDA
                     RPSDSIALALRLQAPILTADELLQHTVIETTDTIGESTGPSGERTSGPLDADALKTYL
                     QNLDPEDFGKFTP"
     gene            14541..15578
                     /locus_tag="VFU75_10200"
     CDS             14541..15578
                     /locus_tag="VFU75_10200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467254.1"
                     /translation="MVPSTPTPSRPTSRTSILKISGSSRRNGVLLGLWVLGAGAAASG
                     QSVTLAGAVTLGYAHPRPLTGATVILHRVTMLVQGPVDSARLDARGQYRLRIPVVDST
                     ANYLISVLHDGVGYFSEPIRPLHSAPGPRLNVDTLRVYDTSVGGPPLTVTKRLISVAA
                     AKGDGSREVLEITVLSNPGEKTRIAPDTLHPVWTGLLPSGALQFSVGQGDFSADAVAR
                     HGDTVALYGAVQPGGGRQLAFSYLIPRGSSPFLVPVDHSLDDMMVLLEDTTARVTGPG
                     LRALGVRLLESRHFAQYQIDHVKAGALVAIDFPHGAFRIESLVPFIAVLAGLVLGGGF
                     WLAVRRPRPAP"
     regulatory      15656..15765
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            15875..16750
                     /locus_tag="VFU75_10205"
     CDS             15875..16750
                     /locus_tag="VFU75_10205"
                     /inference="COORDINATES: protein motif:HMM:NF013648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helical backbone metal receptor"
                     /protein_id="HEU5467255.1"
                     /translation="MRRAALYIGLLLLSACGRGRPQAPGALSVTDDAGRTVSLAASAR
                     RVVSLAPSSTELLFAVGAGPQVVGRTTWCRYPAAALAVPVVGDGLDPNLEAVAATHPD
                     LVVLYRSPSNGTAITQLDQLGIKSVVLRQDLLVDVARDARLLGTLTGHQVAGDSIARL
                     LDSLSALPAAAARARVAIVVWDNPPIVIGGGSFLDELGRLAGAQNVFHDLPQASATVS
                     LESIASRNPDFIAVVDDSSASNTPSFARRPEWRAVPAVRAGHIIHLPGTLFGRPSPRA
                     AEAVAAYARLLNGTK"
     gene            16747..17694
                     /locus_tag="VFU75_10210"
     CDS             16747..17694
                     /locus_tag="VFU75_10210"
                     /inference="COORDINATES: protein motif:HMM:NF013219.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU5467256.1"
                     /translation="MKNAGLVALSLIALALAVILGPTSVPLRDLIHSPIVRDLRVPRA
                     LLGFLVGGALGVSGAGLQALVRNPLADPFLMGLSGGAGLGAVGAIALHLSGPWALPLA
                     AFLGALAAMALVYRLGLVAGATLDPRILLLAGVVVGSFAAAISTAIVSLSEAEQLRNA
                     MLWLWGGLSGASWTTVLVMLIYLPVPLVILVAAARPLDLMALGDEPAAYLGADVARVR
                     RRVYLAASLLTAAAVAVSGVIGFVGLIVPHIGRLIWGHRHRALIPVSFLGGGILLMLS
                     DTLARTVVAPRELPVGIVTALIGVPAFAVLLRRWVGARA"
     gene            17691..18488
                     /locus_tag="VFU75_10215"
     CDS             17691..18488
                     /locus_tag="VFU75_10215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5467257.1"
                     /translation="MILALEHVHARYPGTARDALADVSLGVSPGELVAVLGPNGSGKT
                     TLVRVALGTLAPSAGSVVVSGRRAGEWDRRALARVAAVVTQREEMTFPQRARESVMLG
                     RYPHLPPFAAPRSADHQAVRDALEACDALGLADRWLETLSGGEQQRVRLARALAQEPQ
                     LLVLDEPTASLDVKHEMELFERVRALVDRRKLAAVVITHHVNLAARFADRVLLLSRGA
                     SAGSGSAREVLTRETVERVFEWPIAIQAFDDRPQMIPLRRELPTDRS"
     gene            18491..>19212
                     /locus_tag="VFU75_10220"
     CDS             18491..>19212
                     /locus_tag="VFU75_10220"
                     /inference="COORDINATES: protein motif:HMM:NF019335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HEU5467258.1"
                     /translation="MRVVVLMASAIVVRSVSAQTPGDTFKLAPVVVTATGVPTRADQL
                     PVSVTVLKGSDLAARGIRTVSQALRLVPGAAVVETSSFGSQTSLFLRGGESDYAKVLV
                     DGVPQGQPGVGFDYANLTTDDIDRIEVVSGPVSVLYGSDAVTGVVQIFTKTGQGGTQG
                     TVSAHAGTYGSADYALSAGGGNERASWSLSGSRFTSDGSLPVNNHYRNGAVNGRLVVR
                     PGDSTEAALSVRYDDGLYFFPTT"
BASE COUNT         2877 a         6359 c         6813 g         3163 t
ORIGIN      
        1 atgggactgg ggatcgacga tccgttcccg gaaacctccg agtccgtggc gcaggtcttg
       61 cttgcggtac accggagcta cgcggcggcc gtgatgcctg tcatcgcccg gatccacgcg
      121 ctggcgcaca ttacgggcgg cgggattccc ggcaatctga tccgcgtgct ccccgatggc
      181 tgcgaggccc tggtcgaggc gggagcgtgg gagtggccgc cgctgttccg cgtactgatg
      241 cgggcgggga aggtatcgct cccggaaatg cgggacgtgt tcaacctcgg aatcggcatg
      301 atcgcggtcg cggggcggga tgacgtggag ccggtggtcg cggcggctgc ccgggccggc
      361 attccggcat gggtgatcgg tgaaattcgg cccggcaaga cgggcgtgca gttctcacag
      421 cggtaacctc actgggaggc tcgggtatgc gtcgtatcgg tatcctgctg ggactggcgc
      481 tcgcggccgg cgcgccgctg gcggcccaat gcaacacgga ctgccaacgg gcggaggacg
      541 cactcaagtc cttccatccc gtggccgggc tgatcgtcag cggtggcaat ccggtgctcg
      601 gatcgggtgg caccctcggc gggctgggcc acttcttcgt atcggcccgc gtgaatgccg
      661 tgaaggtggc gctcccctcc accagctcca ccgatacggc ggcaacctcg gcggtggttc
      721 ccgcgcctgt cgtcgaagcg ggcctcggta tctggaacgg gctcggtggg ggattgctct
      781 ccgtcgacgc cctcgcgtcg gcgacgcttc tgccgacgac gcaggtctcg aacctcaagg
      841 tggattccag tgcgaccaag gtcgggtccc tcgcgctcgg cctgggctac ggactgcgcg
      901 tcggcatcat caggggatcc ttcttcgtgc cgtccgtttc ggtgagcgtc atgcgccgca
      961 cgctgccgcg cctgacctac gggacgctcg gggccgtgcc ctcgaccgac agcgcctttt
     1021 cgttcgacac cgatctcaag gccacgaact tccgagtcac ggcgggcatg cacctgctgc
     1081 tgctcgacgt cgcggcgggc tttgggtacg acatctatac gtcgaacgcg cacatcgcgt
     1141 actacaactc tcccatcacc gtcgcgcacg atacgctgaa cctgaacaac aagcgcgaag
     1201 tgatcttcgt gaatgccggg atgaacctca tgctggtcaa ggtggtcggc gagctgggct
     1261 atcagaccgg caaggaccag accttgtcca ccaactacca gggtctcgac cccaaggcgg
     1321 ggcatgtctt cggtggagtc ggcgtccgta tcagcttctg acgtcgcggg cgatgccgcc
     1381 gcgatgaccc gcgccctcga cctcgcgatg cgaggttggg ggcgtgtggc tcccaacccc
     1441 atggtgggcg cggtggtgct gcagaacggc aaggccgtcg ccgaggggtg gcacgcggag
     1501 ttcgggggac cgcacgccga ggtggccgcg ctcaatgccg cgggcggcca ggcgcgcggc
     1561 agtacgctcg tcgtgagcct ggaaccgtgc cgccacaccg ggaagactcc tccctgtgtc
     1621 gacgccatac tcgcggccgg ggtgcggcgc gtggtggccg cgattcccga tccggacccc
     1681 caggcgggcg gcggtctcga gaaactgcgg ctcgccggtg tcgacgtcac gatgggaatc
     1741 gaaatcgagc gggcggcgtc gctgaacgcg gcctttctcc actcccgcac cgatcacgag
     1801 cggcccttcg tcgcgctgaa gctcgcgacc tccgtcgacg gccgcattgc cgatcacgcc
     1861 gggcgatcgc agtgggtgtc cggagaggag gcgcgggact acgtgcactg gctgcgggcc
     1921 gggcacgacg cgatcgccgt cggcggcacc acggccctgc gcgacaatcc ccggctgacg
     1981 gtccgcggtg ccgtgacgcc gcgccggccc cccgtgcgcg tggtgttcga tcggcgggcg
     2041 atgctgaacc ccacggtcac gctggtgcag acggcgcgcg aaatcccgac gtgggtgatg
     2101 gcgtcccccg acgcgccggc caccaacgcc tcggtgctcg atgccaacgg tgtgcgggtg
     2161 ttccgtcccc tcgatgtgca ggccggcctc agggagctcc gggcggccgg gatccagtcg
     2221 gtgttatgtg aagggggagg ggcgctggga gcgaagctgc tctccctggg gctcgtggac
     2281 cggttgtact ggattcaggc cccgttgtgg ctcggggatg acggcattcc ggccttcccc
     2341 ggagtcgcga acgtcgatat agaccaggcc ccgcggtgga ttcccgtgga gcgtaaggcg
     2401 ttgggggcgg atacgttact cgtcttggac cgcaggctat gtttaccgga atagtcaccg
     2461 ccgttgggcg ggtcaaggcg agccgccggg agccgggagc cgggagccgt ccctccggag
     2521 tggcgctcac catcggcgcg ccgtaccggg gcctcaagcg cggagaaagc atcgccgtca
     2581 acggcgcgtg cctcacggtg gagaaggtgg tgaagggcgg gttcacggtg cacgccatcg
     2641 ccacgacgct cgatcggacg ctgatcgggg aatatgcggc gggccggcag gtgaacctcg
     2701 agcgtgcgct gaaggcgagt gatcgcctgg gtggccacat cgtgcagggg cacgttgacg
     2761 gggtgggccg ggtgattgcc gtggccgacc gggacgacgc ccggctgctc gatctggagg
     2821 tgccgggcga cgtccgcgag gtcacggtgc cgcacgggtc gattacggtc gacggcgtca
     2881 gcctcacggt gaacgagctg ccgccgtcgg gggtggtaca ggtgtcgctc attccgttta
     2941 cgcggaccca taccacgttg ggcgccgcga gggtgggcga ccgggtccac gtggaagggg
     3001 acgtcctcgg gaagtacgtg aaagcattat gtcgcacgaa ctcgtagaac aggcgatcgc
     3061 ggacatccgc gcgggcaagc tggtcatcgt cgcggatgac gaggatcggg aaaacgaagg
     3121 cgatctggtc ggggcggccg ctcgggtctc cccggaaatg atcaattaca tggcgaccca
     3181 cgggcgaggt ctcatctgcc tcactctgac gcccgaccgc tgccaggccc tcggtctgtc
     3241 gcagatgacc gagcacaaca ccgagtcccg cgaaaccgcg ttcacggtga gcgtggacgc
     3301 aggcgctcgt ttcggcgtga cgacgggcat ctccgcggcg gatcgcgcgg cgacgattcg
     3361 cgtcgccatc gatccggctt cggagccgtc ggacctgcgc cgtcccggtc acgtattccc
     3421 tttgcgctcg cgccccggcg gggtgctgca gcgcgtggga caaaccgagg cctcggtgga
     3481 cctggcgcgg ctggcgggac tgtatcccgc cggggtgatc tgcgaaatcc tgaacgccga
     3541 cggcacgatg gcgcggcggc ccgagctcga ccgcttttcg gccgagcaca agctcacgtt
     3601 catcacggtc gctcagctgg tgcagtaccg gctggccaag gagcgcctgg tgcaccgcgt
     3661 ggcgagcgcg cgcctcccca ccgccgcggg tgagttcaag atcatcggct atcggaatga
     3721 cgtggatgaa gcggagcacg tcgccctggt gtacggtgac gtcggcgggc agcccgccgt
     3781 catggtccgc atgcactcga agtgcctgac gggggacgtg ttcggctccc agcggtgtga
     3841 ttgcggctgg cagctggaga gcgccatgca caccatcgcc cgtgagggcc gcggcgtgat
     3901 cgtctacctg gatcaggagg gccgcggcat cggcctcctg aacaagctca aggcctacga
     3961 gctccaggac gccgggaacg acaccgtcga ggcgaacgag aagctcggct tcaagcccga
     4021 cctcaggaac tacggcatcg gcgcgcagat cctgctcgac ctggggttgt cgtccatccg
     4081 cgtgctcacc aacaatccga agaagctggt cgggctcgaa ggctacggtc tcgagatcgt
     4141 cgagcgcctg cccatcgtgc ccccgccgtc cgacgagaat cgcgcgtatc tggacgtgaa
     4201 gcgcgacaag ctcggacacc tgctcaccca ctgacgtgac cgaattccgt ggcactccgc
     4261 gcgcaccggc cacgcggcgc gtggcgctgg tcgtgagccg ctaccacgag ctgatcaccg
     4321 gcaagctgct cgagggcgct cgtgcctgct gccgcgagca cggcgtgaag gacgaccacg
     4381 tcgacgtgct ctggaccccg ggagcattcg agctccccgt ggtcgccgag gcggcggcgt
     4441 cgagcgggag atacgccgcg atcgtcgcac tcggctgcgt gatccggggc gaaacgccgc
     4501 acttcgacta cgtcgccggc gaggcgaccc gcgggctcgg gaacgtcgcc ctcgcccacc
     4561 aggtgccggt ggggttcggg gtcctcaccg tcgacaccca ggagcaggcg gaagcccggg
     4621 ccggcgggag cgcggggaac aagggattcg aagcggccga ggcggcgctc gcaacggcag
     4681 acctgctggc gcaactccct cgtgctgcgc gctgaaacca aggcccgggc ccgcgccctg
     4741 cagatccttt atgcctggga tctctcgggg aggccgtccg tcgaggcggt ggtggcgcgc
     4801 ctgggcgtgg ccttcggcca tgagccggaa ggctacgacc ggggtgccga tctggcggcg
     4861 cgtgccgccg cgggcacgga agattacgac cgccgcatgg ccgacgccgc ggagcattgg
     4921 cggctcgagc gggtcggcgc cgtcgagaag aacatcctgc ggatcgccat ggcggagctg
     4981 gacgaaggcg tcaccccgcc gagagtcgtg atcgacgagg ccgtccggct cgcgcactgg
     5041 ttcgcgggcc cgaaggcgcc gggcttcgtg aacggcgtgc tggacaccgt ggcccgtgag
     5101 cagggcctgc tgtgagaatc ctgctggtca attggaatga ccgggagaat ccgcaggcgg
     5161 gcggggccga gattcacctg cacgagattt tcggacggct ggcgcgcgcc ggacaccaga
     5221 tcgacctcgt cgtgagcggg tgggcgggcg cgagcgcgca ggcgacggtc gatgggatgc
     5281 gggtgcatcg ggtgggggac cggaacacct tcgccatgct gggacgcggg gcggtccgcc
     5341 ggcggctggc ggccaacacg tacgacatcg tggtcgaaga catcaacaag cttccgctct
     5401 tcctcgcctc ggcgacccgg ctcccgttct gtgtgatcgt cccgcacctc ttcggcacca
     5461 ccgtcttctc cgaggccagc tggccggtgg ccgctttggt ctgggcggct gagcgtccca
     5521 ttccgtgggc gtaccggcgt gccgcgtttc acgcgatctc ggacagcacc aaggcggacc
     5581 tggtcgcgcg tggcgtgccg gccgagcgga ttgaagtcat ctatccggga gttgacgcga
     5641 actggtaccg gcccgattcg tcggttcggc gcgcggcgcg gcccacgttc ctgtacgtcg
     5701 gtcgcctcaa gcgttataaa ggagtggagt acgcgttgcg ggcgctggcg gcggcgcggg
     5761 tccagggcgg cgacctgcgg ctcgatatcg cgggcaaggg ggacgaccgc ccccgcctcg
     5821 agcgcctggc ccgcgacctg gggttgatgg agacggtgcg attcctcgga ttcgtggatg
     5881 acgaaaccaa gcgccggctc cttcgcgaat cgtgggctgt cgtcttcccg agtccgaagg
     5941 aaggatgggg catcaccaac gtcgaggcgg cggcgtgtgg cacgccggcc ctcgcctccg
     6001 acagccccgg tttgcgggaa tccgtgcggg acggggagac cgggtatctg gtgccgcatg
     6061 gtgacgtgac cgcgctcgcc gaccggatgc tggtcctcgc gaacgatccg cgccgggtcg
     6121 agaccctggg cgccgcgggg cggcgtttcg ccgaaggcct cacgtgggac cgcgcggcgg
     6181 cagaaacggg gcggcacctg gaacgcatcc gttcatcgca tggtggggag aatcgttagt
     6241 ggcgaatccc aaagttcggg atctcctggc gcggaaggcc gagccgctgc agctcgaggc
     6301 cctgaccggg gagctcgggt tcgacaggga gattcccgcg cccgaagcct cgagcccggg
     6361 tttggtcctc gccggctata ccgcccgctt cgtcgggaac cgcatccatg tgctcgggga
     6421 aacggagatc agctacctct cgtcgctcga gccggatgcc cggcgtgggt gcctccagaa
     6481 tctcttcaag ttcgagctgc cggtcgtcgt catcaccaaa ggccagtcgc ccccggcgga
     6541 gctgctcgag ctcgcccagg cgcgcggcgt ggccctggtg cggacccagc tgaagacgtc
     6601 ggaattctac cggcgcctca agccttttct cgatgacgcg ttcgctccca ccacgacgac
     6661 gcacgcctcg ctcgcggacg tcttcggcgt gggcctcctg ttcatggggc gctcgggaat
     6721 cggcaagagc gagtgcgtgc tcgacctcgt cgagcgcggc caccgtctgg tggcggacga
     6781 cgtcgtccac gtcacccggc gagggaacga cgtgttgatc ggacgcggcc acgagctctc
     6841 ccggcatttc atggagatcc ggggggtggg gctgatcgac atccaggcgc tcttcggcat
     6901 ccgggccgtc cgccagcaga aacgcatcga agtggtggtg cagctcgagg attgggacgc
     6961 gaatcgggag tacgaccgga ccgggctgga ccgccaggaa accacgctgt tgggcgtttc
     7021 tctgcccctc gtgacggtcc ccttgaaccc cggcaagaat ctgacggtta tttgtgaggt
     7081 ggtggccatg aaccacctcc tgcgatatgg cggcgtagac tccgccaagg agttcaacga
     7141 ccggctgatg cgcaccatgc ggcagcgccg ggacgtcggc acgtatctcg aggaagacga
     7201 tgagtaactc gctgcggggc gtcgtggtct cccacgccgg actcgcgcag tcgctggtga
     7261 cggccgccga gaccatctcg ggggtcgcgg gcgcgctcgt gccggtgacc aacgaaggat
     7321 gcgacggcgg atcgctgaag gcgcgcatcg gcgaggccat cggcgatggc ccggccgtcg
     7381 tcttcgtgga cttgccgggc ggctcgtgtc tcgcgggcgc ggtgcggatc gcgcgggacc
     7441 agggccgcgg catcgccgtg gtcaccggcg tgaacctcgc catgctcctg gacttcgtct
     7501 tccatcggga cctgccgccg gctgaggcgg cgcagcgagc cgtcgacgcg ggggggcgcg
     7561 ccatccggat accgccggcg tgagtctcgc ggtggtgcgg gtggatgacc ggctcatcca
     7621 cgggcaggtg gtggtggggt ggggccagcc gctggggctg ggcttcatcg ccctggtcga
     7681 cgacaaggtg cggtcgagcg gctgggagca ggacctctac cgcatgggcg tgcctcccgg
     7741 gatagaggcg atctttgcct cggtggacga agccgcccgc cggtacgaag actgggtgaa
     7801 ggatgcccgg atcggactcg tcctcacggg ggacatcgac accctcatcg ccctcagcga
     7861 caaggttccc aaactccgcc gcgtcaccct cggcggcatc catcacaaac cggggcgctc
     7921 cgagcgcctg cggtacgtct acctcaccgc ggacgaggtc gcccaattgc gcgagctcac
     7981 gaaacgcggg atcgaggtcg tcgcgcagga cgtccctacg gcgcgtgccg tccccctcgc
     8041 ggaattcgcc aagtgatgcc gcacgggtgg gagctcctgg ccttcggggc cgtcgtcggg
     8101 ctcgacctgg tgtccttccc gcagatgatg ctggcgcgac cgatcgtggc gggcgcgggc
     8161 gcggggctgc tcctgggcga cgtcggcgcg ggacttcgca tgggcgtgct gttcgagctc
     8221 ttccagtacg acattctccc cgtcggcgcg actcgctatc ccgagtatgg gcccgccacg
     8281 gtgatcgcgg tgggcgccgc gcacgccttc gcgctgccgt tcgggatcgg gctcgccacg
     8341 gtcgtcggcc ttttcaccgc gatgatcggc ggtatctcgt tgcagatcct gcgacgtctg
     8401 aacacgcgcg cggtgaccgc ggcggccccg gcgctggagc gtggagacgt ccgggcgctg
     8461 taccggttgc atgcctccgg cgccgcacgg gacctggcgc gcgcggtgct cgtcaccgcc
     8521 ttcggcctcg tgctggtcgc gatcaccagg gagttcgtgg tgccggcgct ctcgcaccgc
     8581 ggcgagcgcc tcctctcggt ggcggccacc ggtgccggcc tcgccgcggg tgccgccggc
     8641 accctgcgcc tggtcgggct cggcactcac ctcaaatggc tcgccgtggg actggtgggt
     8701 gggatcgtgc tcgcatggct gacgtgaagg ctcttccccg gtcgcggctc gcgatctcat
     8761 tcctgcgcct gttcaccgtg caggcgtcgt ggaactatga gcgcctcatc ggcgtcggga
     8821 ccggctttgc ggccgagccg ctcctccggg gtctggactc cgagcggtat cgcgcggcgt
     8881 tgggccgggc gtcccggtac ttcaactccc atccctatct gagcggtgcc ggggcgggag
     8941 cgttggcgcg tgcggaagtg gacggggttc cggctgagca agtcgagcgt ctccgcgggg
     9001 ctctggcggg tccgctggga tcgatcggcg accgcctcgt ctgggcgggg tggctgccga
     9061 tgctctcggc gttggcactc accggggtga cgtggggccg cgggctcacg gccgtcgcct
     9121 ggttcctggt cgtgtataac ctggggcacg tggctctgcg ctggtgggcc ctgcgcgctg
     9181 gatggaacta tggtccccgc gtagcggcgg cgctgcaaga gccgtggatg cgccgggcgg
     9241 gacagtacct cggacccgcg acggcgctgg cgctcggcct cgcgctgccg atggtggcgc
     9301 aatggtcggg ggacgcgttc cggggatggc cgcgcatcat gctggcggtc gccgcggcgg
     9361 tggccttcgg gttgttgcgg tggcgtcccg atatcctcag cggactcaga ctggggctcg
     9421 tgctcgtcgt gatcgccctg atcgcgggga tgctgtggtg atcgagcgcg aagccctgat
     9481 cgtcaacccg ctcggcgtgc acgcgcgccc ggcggccgag ttcgtgaaga ccgcgaaccg
     9541 gttctccgcc gcggtcgaaa tccggcgcgg ggagatgtcc gtgaacggca agagcatcat
     9601 gggcgtgatg atgttggccg cggaacaggg cgcgaccatc gtcatcagcg ccaatggcga
     9661 cgacgccgaa gccgccgtgg aggcgctctg cgccctggtg gcggccggct tcaatgagat
     9721 ggaccttacg gaatgagcga tcgcacactg cggggcatcg gggtgtctcc cggcatcgcc
     9781 atcggtccgg cggtatcggt ccagacgacg ttgccggagg tgccgcaccg cgtggtgctg
     9841 cgcacgcaag tcgaaaaaga ggtccgccgc cttcgcgcgg cggtccgcga cgtgcgccgt
     9901 cacctgacgg agcttcgcaa acgcgccgag gtcgaggccg gcgcggacga agcccggatc
     9961 ttcgatgccc agatcctgat gctcgaggac aaggcgttca tcgggtcggt cgaggagctg
    10021 atccggaaga atcacctcac cgccgagaag gcgtacgaat accaggcgct cgaagtacgg
    10081 gacctgtggg cggcctcgac caacaccagg ctcaaggaac gtctggccga cctgaacggc
    10141 gtcaccatcc aggtggttcg gcacctcctc cacaagggcg accttgtggc gggcggcatt
    10201 cgggagccgt cgatcctggt ggcgcacgag cttgcaccgg gcgtcacggt gcaattcggc
    10261 cgggatcggg tgctcggtct cgtgagtgag gagggcacga ggacgtcgca cgccgcgatt
    10321 ctcgcgcact cgatgagcat gcctgcggtg atgggactcg ccggcgcact cgagcgcatc
    10381 aagaccggga ccatgctgat cgtggacggc acccggggcg tggtcatact cgacccgagc
    10441 aaggaagaaa tagccgcggc gcgcgcgatg gacacccgtc gcaagcagat cgcccgccag
    10501 gcggaagagg gcgcgaacct gcccgcggtc acgaccgacg ggattcacat caacgtccgc
    10561 ggaaacgtcg atctgcccga ggagatcaag gccgccaagg aacatggcgc cgaaggggtg
    10621 ggactcctgc gcacggaatt cctgatcacc ggacacacca aacttcctac ggaggacgag
    10681 caggcgaagt atttccggaa ggtgggggac gcgttcccgg gctatccgat cgtgatccgg
    10741 acctacgatc tgggaggcga caaattcccg gcgccgttcc gcgcgccgcc ggaggccaac
    10801 ccgttcctgg gatggcgggc gatccgggtc tgcctcgatc agcccgagat gttccgcgtg
    10861 cagctgcggg ccgcgctccg cgccgcggcc cacgttccca ttcacctcat gattccgctc
    10921 gtcacccggc tgtcggaggt ggaaacgacg cggcagatgg tggcggaaga gggccagctg
    10981 ctcaaacggg cgggcatcca ggccgcggaa accgttccgg tgggcgtgat ggtcgaaacg
    11041 cccgcggccg cggtgatggc ggaccgcctc gccgagatat ccgatttcct gtccgtgggg
    11101 acgaacgacc tgacgcagta cacgttggcg gtggatcgga gcaatgcgcg tctcgcgaat
    11161 cgcttcacgc ctcacgaccc gagcgtcctg cgcctgctga agctggtggc cgaagcggca
    11221 cgggccgcgg gcaagcccgc gagcgtgtgt ggcgagatgg cgtcggagcc gatctccgcg
    11281 ttcctgctgc tcgggttggg ctacgagacg ctgtcggtgg ctcctccggc gttaccgctc
    11341 atccgctggc tggttcgaca ggtgagtcgc gagcaggcga tgtcggccgc ggcggcggcc
    11401 ctggccgccc ggtccgcgga cctggcgctc gacatcttgc gttacgcatt ggcggaagcc
    11461 gtggacatga aactcctgga tcccgacgcg cggttgcccg gagggcgcac ctcggctagc
    11521 ttgcccagct aaagggagac gcacctacat gttagacgct cgccacgtgt tcacgtccga
    11581 gtccgtcacc gaaggccatc cggacaagat cgcggaccag atctcggacg cgattctcga
    11641 cgctattctg accaatgact ccgcggcccg ggtcgcctgc gaaacgctgg tgacgacggg
    11701 gatggcggtg gtggccggcg aaatcacgac gacgacgtac gtccacatcc ccgacatcgt
    11761 ccggggcacg atcaaggcga tcgggtacac ggacgcaagc tacggcttcg acgccgagac
    11821 ctgcgcggtg ctgacgacca tcgaccgtca gtcggcggac atcgccatgg gggtcgacat
    11881 cggcggggcg ggcgaccaag gcatgatgtt cgggtatgcc acgaacgaga cggcgtcgct
    11941 gatgccgttg cccatcgtcc tggcgcaccg gatggcggag cggctggcgg cggtccggaa
    12001 agacggcacg ctcccgtatc tccggcccga cgggaagacc caggtcagcg tggtgtatga
    12061 gggctcgcgc ccggtcggca tcagcaaggt cgtcgtctcg acgcagcatt cggaccggat
    12121 caaggcgaac gagctccgcg aggccgtcat caagacggtg gtgacgcccg tgctgaccga
    12181 cgccggcttc aaggtcgccg acgccgacat cctggtaaat cctacgggcc gcttcgtggt
    12241 tgggggcccg cagggcgacg ccggtctcac cggccgcaag atcatcgtcg acacctacgg
    12301 cggcatggcc cgccacggcg gcggcgcgtt cagcgggaag gacccgtcca aggtggaccg
    12361 gtccgccgcc tacgcgatgc ggtgggtggc caagaacctc gtgcaggcgg gcctcgccgc
    12421 tcgctgcgag gcgcaggtgg cgtacgcgat cggcgtcgcg gatccggtgt cggtcatgat
    12481 cgacaccttc ggtaccgggc aggtgcccga agcagccttg gaagaggccg tgcgggaagt
    12541 ctttgatctc acgccggccg ggatcatcaa ggcgctcgag cttcgcaagc ccatctacaa
    12601 gcccaccgcg gcctacggcc acttcggccg tgaacccggt gccaacggtc tcttctcctg
    12661 ggaacggacc aaccgcgtgg aggagctgcg cctggcggtg cagcgctcca ggcactaccg
    12721 ctcgaacaag gttacggcat gacgccttcc accaccgctg tgtccgagac gtccgacatc
    12781 aagaacccgg cgctcgccga cgaaggacgc cgccgggccg agtgggccga gcgctcgatg
    12841 ccggtactgc ggcaggtgcg ggagcgtttt gcccgcgaga agccgctggc ggggcggaag
    12901 ttgtccgcct gcctccacgt caccagcgag accgccaatc tgatggtgac gctcaaggcg
    12961 ggtggcgccg atgtcgcgct ctgcgcgtcg aacccgctct cgacgcagga cgacatcgcc
    13021 gcgcacctcg tgacgcacca cggcatcaag gtcttcgcca tcaagggcga ggaccacgag
    13081 acctactacg agcacctccg ggcggctttg gcccaccagg cggatgtcac gatggacgac
    13141 ggcgccgatc tggtcggcgc gctgcacatg atcgcgctca atcgcctcga cgacctcgcg
    13201 ccgccggtgc ggcgctgggt cgagaagatg tcggcggccg agcgcggggc gctggtcaag
    13261 cgggtgatcg gatccactga ggaaaccacc accggcgtca tccgcctcaa ggcgatggcc
    13321 aaggatgggg tgctccagtt cccggtgatc gccgtaaacg actcggacac gaagcacctg
    13381 ttcgacaacc ggtacggcac cgggcagtcg acgctggacg ggattctccg tgccaccaat
    13441 ctgctgatcg ccgggagcac cgtggtggtg gcggggtacg gatggtgcgg ccgcgggttc
    13501 gcgatgcggg cacgcggggc aggggccatg gtcatcgtca cggaaatcga cccgctgaaa
    13561 gccctcgaag cgcagatgga cgggttccag gtggcgccga tggcggaggc ggcgaaagtc
    13621 ggtgacatct tcgtcacgct gacgggcaac gtgcacgtca tccggctcga gcacatgaag
    13681 gcgatgaaag acggtgcggt catcgcgaac tcgggacatt tcaacgtcga aatcgacctc
    13741 gacgcgctga agggggcggc gacaggcccc gatcacgtgc gcgatctggt cgagggttgg
    13801 acgctcggcg gcaagaagat cttcgtgctg gcagacggcc ggctggtgaa cctcgcggcg
    13861 gcggaaggtc accccgcgag cgtgatggat atgtccttcg cgaaccaggc cctggcggcg
    13921 gagtacgtcg tcaaggagtt caagcagctg gggaaggacg tccatcgcat cccccgcgac
    13981 ctggaccagg acatcgcccg cctcaagctc gccggcatgg gtgtccggat cgacacgctc
    14041 acggcggagc agcagaagta tcttgcctcg tgggatatgg ggacttgagc caacccaaat
    14101 gaaagaagtc cgcgtcgcca atctgggtct cgaccggacc aacaacaccc ccgtggtgat
    14161 tctgcgggag tcggacggcg agcgggtgct tccgatctgg atcgggccgg ccgaggcgag
    14221 cgcgatcgcg atggagctgg cgggtgtgaa gtcatcacgt cccctgacac acgacctcct
    14281 gaagcaggtg atcgtcggcc tgggcgccga gctgcggaaa atcatcatca ccgcggtgaa
    14341 agacagtacc tactacgccg agctgcacct gtatcgaggc gaccaggtga tccagatcga
    14401 tgcccggcct tccgactcga tcgccctcgc ccttcggctg caggcaccga tccttacggc
    14461 cgacgagctg ctccagcaca ccgtgatcga aacgaccgac accatcggcg aaagcacggg
    14521 cccctcaggg gaacggacca gtggtcccct cgacgccgac gccctcaaga cctacctcca
    14581 gaacctcgat cctgaagatt tcgggaagtt cacgccgtaa cggcgtcctc ctgggcctgt
    14641 gggtcctggg agctggagcc gcagcgagcg gacagtcggt caccctggcc ggtgcggtca
    14701 cgctcggcta cgcgcatccg cgccccctca ccggtgcgac cgtcatcctg caccgggtga
    14761 cgatgctggt gcagggcccc gtcgactcgg cccgcctgga cgcccggggc caatatcggc
    14821 tccgcatccc cgtggtggac tcgaccgcca actatctcat cagcgtgttg cacgacgggg
    14881 tcggctattt ctccgaaccc atccgtccgc tgcattccgc gccggggccg aggctgaacg
    14941 tcgacacgtt gagggtctac gacacgtcgg tcggtggacc gccgctgacc gtgaccaagc
    15001 ggctcatctc ggtggcagcg gcgaaggggg acgggtcacg ggaagtcctg gagattaccg
    15061 tcctgtcgaa tcccggggaa aagacccgga tcgcgccaga caccctgcac cccgtgtgga
    15121 cggggttgct gccctccggc gcactgcaat tctcggtggg tcagggagat ttctccgccg
    15181 acgccgtggc tcggcacggc gataccgtcg ccctgtacgg cgcggtccag ccgggcggcg
    15241 gacgccagtt ggcgttcagc tatctcatcc ctcgcggttc gtcaccgttc ctggtgcccg
    15301 tcgaccattc tctcgacgac atgatggtgc tgctcgaaga caccacggcg cgcgtgaccg
    15361 gtccgggact gcgtgccctc ggcgtccgcc tgctcgagag ccgccatttc gcccagtacc
    15421 agatcgatca cgtgaaggcc ggcgcactcg tcgcgatcga ctttccgcat ggcgcgttcc
    15481 gcatcgagtc tctggtgccg ttcatcgccg tgctcgcggg cctggtgctc gggggcggat
    15541 tctggctggc cgtgcgacgg ccgcgtccgg ctccctgacc gatcactcgc ttgctctttc
    15601 agccgcactc cgctagcata gcctcgtagt cacaaaacgc gaacgcggga tctacggaag
    15661 ccggtgcaag tccggcgcgg ccccgccact gtaacgggca gcagatgtcg gctcacgcca
    15721 ctgggaaacc gggaaggcca gccgaaacgc ccgaagccag gagacctccc cgttcgcgcc
    15781 acctgcaatt ccctcgcggg agggatggtg gcgctgcgca ctgaggcgca gacgctcgcc
    15841 catctctggt ccgccgggga tgggtttttg attcatgcgc cgggcggcac tgtacatcgg
    15901 actgctcctg ctgtcggcgt gtgggcgagg acgccctcag gccccgggcg cgctgtcggt
    15961 caccgatgac gccggccgca cggtttccct cgccgcctcc gcccgccggg tggtctccct
    16021 cgctccttcc tccaccgagc tgctcttcgc cgtcggggcc ggtccccagg tcgtgggtcg
    16081 gaccacctgg tgccgctatc cggcggcggc cctggccgtc ccggtcgtgg gggacgggct
    16141 cgatccgaac ctcgaggcgg tcgccgccac gcatcccgat ctcgtggtcc tctaccggtc
    16201 tccttccaac gggacggcca tcacccagtt ggatcagctc ggcatcaagt ccgtcgtgct
    16261 ccggcaggat ctgttggtcg atgtcgcgcg ggatgcccgg cttctgggga cgctgaccgg
    16321 gcaccaagtc gccggcgact cgatcgcccg cctgctcgac tcgctgagcg cgctgccagc
    16381 cgcggccgcc cgcgcacgcg tcgcgatcgt ggtatgggac aaccctccca tcgtgatcgg
    16441 gggcggctcc ttcctggacg agctgggcag gctcgccggt gcgcagaacg tgttccacga
    16501 cttgccgcag gcgtccgcca cggtgtccct cgagagcatc gcctcgcgca atccggattt
    16561 catcgccgtc gtcgacgact cgagcgcatc caataccccg tcgttcgcca ggcgtccgga
    16621 gtggcgggcg gtcccggcgg tgcgcgccgg gcacatcatc cacctgccgg gcacgctctt
    16681 cggacgcccc agtccccgtg ctgccgaggc cgttgcggca tacgcgcgac tcctgaacgg
    16741 gaccaagtga agaacgccgg gttggtcgcg ctctcgctca tcgcgttggc tctggcggtg
    16801 atcctggggc cgacgagcgt cccactccgt gacctgattc attcgcccat cgtccgggat
    16861 ctccgggtac cgcgtgcact cttgggattc ctcgtgggcg gtgcccttgg cgtctcaggt
    16921 gccggcctgc aggcgctggt tcgcaacccg ctggccgacc ctttcctgat gggactgtcc
    16981 ggcggggcgg gactcggcgc cgtgggcgcc atcgccctgc atctctccgg accctgggcc
    17041 ctcccgctcg cggccttcct tggcgctctg gcggcgatgg cgctggtgta ccgcctgggg
    17101 ctcgtcgcgg gcgcgactct cgatccccgt atcctgctcc tggccggcgt cgtggtcgga
    17161 tcattcgcgg cggcgatctc caccgcgatc gtgtcgctct cggaagccga acagctgcgg
    17221 aacgcgatgc tgtggttgtg ggggggcctg tcgggtgcgt catggaccac ggtactggtg
    17281 atgctgatct acctcccggt gccgctcgtc attctcgtcg ccgcggcccg gcccctggac
    17341 ctgatggccc tgggtgatga gcccgccgcc tatctcggtg cggacgtggc gcgcgtgcgc
    17401 cgccgcgtct acctcgcggc gtccctgctc acggcggcag ccgtcgcggt ttccggtgtc
    17461 atcgggttcg taggcctgat cgtgccgcac atcggacgcc tgatctgggg tcaccggcat
    17521 cgtgccctca tcccggtgtc gttcctgggt ggcgggatcc tcctcatgct gtcggacacc
    17581 ctcgcgcgga ccgtggtggc cccacgggag ctgccggtgg ggatcgtgac ggcgttgatc
    17641 ggcgtgcccg cgttcgcggt gttgctgcgc cgttgggtgg gcgcgcgggc atgatcctcg
    17701 ccctcgagca cgtacacgcc cggtacccgg gaaccgcgcg cgacgccctc gccgacgttt
    17761 cgctcggcgt ctcgccgggg gagctggtgg cggtgctggg acccaacgga tccggcaaga
    17821 ccaccctggt gcgggtggcc ctcggcaccc tcgcgccctc tgccggctcg gtggtcgtgt
    17881 ccggcaggag ggcgggggag tgggatcgac gggcgctggc ccgggtggcc gccgtcgtga
    17941 cgcagcggga ggagatgacc tttccccagc gtgcccgcga gtccgtcatg ctgggccgtt
    18001 atccgcacct cccgccgttc gccgccccgc gaagcgccga ccaccaggcc gtccgggacg
    18061 cgctcgaggc gtgcgacgcg cttggactcg cggaccggtg gctcgagacc ctctcgggcg
    18121 gcgagcagca gcgggtgcgc ctagcgcgtg ccctcgccca agagccccag ctcctggtgc
    18181 tcgacgaacc gacggcctcc ctcgatgtga agcacgagat ggagctcttc gagcgggtac
    18241 gtgcgctggt cgaccgccgc aaactcgccg cggtggtcat cacccatcac gtcaacctcg
    18301 cggctcgctt cgccgaccgc gtcctcctgc tctcgcgggg agcgtccgcc gggtcggggt
    18361 ccgcacgtga ggtcctgact cgagaaaccg tcgagcgcgt attcgagtgg cccatcgcga
    18421 tccaggcttt tgacgaccgt ccgcagatga ttcctctacg tcgcgaactt cccacggacc
    18481 ggagttagga atgcgtgtcg ttgttctgat ggcttcggca atcgtcgtcc gttcggtctc
    18541 cgcccaaacc ccgggcgaca ccttcaagct ggcgccggtc gtcgtcacgg ccaccggagt
    18601 tcccacccgc gccgaccagt tgcccgtgtc ggtgacggtg ttgaaggggt ccgatcttgc
    18661 ggcccggggg attcgtacgg tctcgcaggc ccttcggctg gtgcctggcg cggcggtcgt
    18721 ggagaccagc tccttcggat cccaaacctc gcttttcctc cgcggtggtg agagcgacta
    18781 cgccaaggtt ctggtcgacg gggtgccgca aggccagcct ggcgtgggat tcgactacgc
    18841 gaatctgacg accgacgaca tcgaccgcat cgaggtggtg agtggaccgg tgagcgtgct
    18901 gtacggatcg gatgccgtca ccggcgtcgt gcagatattc acgaagaccg ggcagggagg
    18961 gacgcaggga acggtgtcgg cacatgccgg cacgtatggg agtgccgact acgctctgtc
    19021 agccgggggt gggaacgagc gggcgagctg gtcgctttcc gggtcccggt tcacgtcgga
    19081 cggatcgctc ccggtgaaca atcattaccg gaatggcgca gtgaacggcc ggctggtcgt
    19141 ccggcccggg gatagcacgg aggcagccct gtcggtccga tatgacgacg gtctgtactt
    19201 ctttccgacg ac
//
LOCUS       DASUBP010000273         4291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_119228, whole genome shotgun sequence.
ACCESSION   DASUBP010000273 DASUBP010000000
VERSION     DASUBP010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119228"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VFU75_10225"
     CDS             <1..460
                     /locus_tag="VFU75_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467259.1"
                     /translation="VSGFSLPRESIHTRLLLTQGGERAGSVFVMARAPSREGPETPLE
                     MLNRPEGFFPFLPDDRKEVLLVSRAQTIALTIPAAGGKPAPGSIEIGLEVALVDGTVW
                     NGVAEVVLPQSMSRPIDYLNAGGGSFFAVTTNHATHLVNRAHVLYARPTD"
     gene            465..2039
                     /locus_tag="VFU75_10230"
     CDS             465..2039
                     /locus_tag="VFU75_10230"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HEU5467260.1"
                     /translation="MARLDPFIDLLLKNGGEQLVLVPDEAVGMEKQGRQSKLSKQVIT
                     EKQLYALLTEMAPSEAADRIGKGAGATFEYAGGAGLVIVRVTPKNGKLHAQITPGVDA
                     GSGMADSPAEEPAAPPAAESPSGGRKPRLSVVVAPPPMEASTNAPGALPAEFADSRLE
                     GAQRQLGELLHALVHSKSSDLHLRVDEPPIFRTHGEIVRHGEDPLSGEQLDLLLLSIM
                     PERNRVEWRESGDTDFAYEIAGLARFRVNAARDRKGSVGVFRVIPANVVTVEDMGITP
                     EVQKLCYLTKGLVLVTGPTGSGKSTTLCSLIDLINRERSDHIITIEDPIEFVHENKKC
                     LITQRQVHVHTESFKSALRAALREDPDIVLVGEMRDLETIAIALETAETGHLVFGTLH
                     TTTAPSTVDRIIDQFPADRQAQIRIMLSESLKGVIAQTLCKKVGGGRVAAREILLTTP
                     AISNLIREGKTFQIPSIIQTSKKLGMITLNDALLDLVEKKQVEPDEAYMKAVEKAGFA
                     AQLKAKGFKLTVPAEG"
     gene            complement(2064..>4291)
                     /locus_tag="VFU75_10235"
     CDS             complement(2064..>4291)
                     /locus_tag="VFU75_10235"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5467261.1"
                     /translation="GPLGTWPHSAPNYLDLARTSTQIQDLSALSWGTALVGLPGDAVQ
                     APELYATGHLFPMLGVTAQVGRVLGPDDDRPGAPAVVMISDRLWRDHLGGDPSVVNRS
                     VIIDGVPQTVVGVLPHDFQIPIGGQFMSADVWAPMRFEPRQLTTRQMNYLKVVGRLAP
                     GATVATADAEMRGLFQNLVTTYPYLNGDAVRVAPLRTESLASIKTPLLLMFGAVCMVL
                     LIAVINVAALLLARGVQRRRETAIRAAIGATRWETMRPALIEAGVIATVGLVGGVGLA
                     VLGVRTIGALAAARLPQLSGLHVDLRVLGFSVALAFIVALACGAVPAWRGSRVDPQDA
                     LRAGRGSGGGREHHRALRGLVVFEMCLSLVLLLGAGLVLKAFVGLLDKDPGFDASRIL
                     TMSVTTSARAYNTTNPVRGMLEPALTAIRNVPGVEAAASINMMPYQNWGNNSNIRYEG
                     MPPDNPNSMPLVEQRDVSPDFFAVTGQRLLAGRLLLPTDDDRPGSPSVVVVNQALVKR
                     DFHGGDAVGKRFYTGDTTFATIVGVVSNIRNFGPVSDPQPEMYSTYLQSSTMTTNFPL
                     VIRVRGTTPTAVVAGIRAAVHSVDPSAAIADVNPMSEVILRSLGRPRFYFSLLGTFAA
                     VAVALAVAGLYAVLSYAVIQRNRELGIRAALGSSTASIVGLVTRDGLKLVGLGVALGL
                     AASVGVTRLMVFMLYGVSPLDPLTWLGASAVLVGAGLVASVIPAWRASRVDPLVAIQA
                     E"
BASE COUNT          773 a         1481 c         1428 g          609 t
ORIGIN      
        1 cgtgagcggt ttctctctcc ctcgcgaatc gatccacacg cgactcctcc tgacccaagg
       61 gggcgaacgg gctgggtccg tgttcgtgat ggcgcgggcc ccgtcccgcg aagggccgga
      121 aacgccgctc gagatgctga accgtcccga agggttcttc cccttcctgc ccgacgatcg
      181 gaaggaagtg ctcctcgtgt cgcgggccca gaccatcgcc ctcaccatcc cggccgcggg
      241 cgggaagccc gcgcccggat cgatcgagat cggactcgag gtcgcgctgg tcgacggcac
      301 cgtctggaac ggcgtcgcgg aggtcgtgct gccgcaatcg atgtcccggc ccatcgacta
      361 cctcaatgcc ggcggaggtt cgttcttcgc cgtgaccacc aaccacgcca cccatctggt
      421 gaaccgcgcg cacgtactgt acgcgcgccc cacggactga cgacatggcc cgtctcgatc
      481 ccttcatcga tctccttctg aagaacggcg gcgagcagct ggtgctggtg cccgacgaag
      541 cggtggggat ggagaaacag ggccggcaat ccaagctctc gaagcaggtg atcaccgaaa
      601 agcagctgta cgccctgctc accgagatgg cacccagtga ggcggccgac cggatcggca
      661 aaggcgccgg ggcgacgttc gagtacgccg gtggagccgg cctcgtcatc gtccgtgtca
      721 ctccgaaaaa cggcaaactg cacgcccaga tcactccggg cgtcgatgcc ggaagcggca
      781 tggcggattc gcccgccgaa gagcccgctg ccccgccggc cgccgagtcg ccatcgggtg
      841 gccggaagcc gcggttgtcg gtcgtcgtgg ccccaccgcc gatggaagcc tcgacgaacg
      901 cccccggggc actgcccgcc gagttcgccg attcgcgcct cgagggcgcc cagcggcagc
      961 tgggagagct gctgcacgcg ctggtgcact ccaagtcgtc cgacctccac ctgcgcgtgg
     1021 atgagccgcc gatcttccgc acccacggcg agatcgtgcg gcacggggaa gaccccctgt
     1081 ccggcgagca gctcgatctc ctcctgctgt cgatcatgcc cgagcggaat cgggtcgaat
     1141 ggcgggagtc gggtgatacg gacttcgcgt atgagatcgc cgggctcgcg cgcttccgcg
     1201 tgaacgccgc ccgcgaccgc aagggatcgg tcggcgtgtt ccgcgtgatt ccggcgaacg
     1261 tcgtcaccgt cgaagacatg ggcatcacgc ccgaggtgca gaagctctgc tatctcacca
     1321 agggcctggt gctggtcacg gggcccacgg gttcaggcaa gtcgaccacg ctctgctcgc
     1381 tcatcgacct catcaatcgg gagcgctccg atcacatcat cacgatcgaa gacccgatcg
     1441 agttcgtcca cgaaaacaag aagtgcctga tcacccagcg gcaggtgcac gtccacaccg
     1501 aaagcttcaa gtcggcgctc cgggcggcgc tccgtgagga tccggacatc gtcctggtcg
     1561 gcgaaatgcg cgacctcgag accatcgcca tcgcgctcga aacggcggag acggggcacc
     1621 tggtgttcgg caccttgcac accaccacgg cgccgtcgac ggtggaccgc atcatcgatc
     1681 aattccctgc ggaccggcag gcgcagatcc gcatcatgct ctcggagtcg ctcaagggcg
     1741 tgatcgcgca gacgctctgc aagaaggtgg gcggcgggcg ggtggcggcg cgggagattc
     1801 tgctcaccac gccggcgatt tcgaacctga tccgcgaagg caagacgttc cagatcccgt
     1861 ccatcatcca gaccagcaag aagctgggga tgatcaccct gaacgacgcg ctcctcgacc
     1921 tggtggaaaa gaagcaggtg gagccggacg aagcctacat gaaagccgtc gagaaggccg
     1981 gcttcgcggc acagctcaag gcgaagggct tcaagctcac cgtgcccgcg gaggggtgag
     2041 agcagcccac ccctcccgcg gcctcactcg gcctgaatcg ccaccaacgg atcaacgcgg
     2101 gacgcccgcc aggccggaat caccgaagcc accagacccg cccccaccag cacggcggag
     2161 gccccgagcc acgtgagggg gtcgagcggg ctcaccccgt acagcatgaa caccatcagc
     2221 cgcgtgaccc ccacactcgc ggcgagcccc agcgccacgc cgagccccac cagtttcagg
     2281 ccgtcccgcg tcaccaggcc gacgatcgag gccgtggagc tgccgagcgc ggcgcggatg
     2341 cccagctcgc ggttccgctg gatgacggca tagctcaaga cggcatagag acccgcgacg
     2401 gcgagcgcga cggcgacggc cgcaaacgtc cccagcaagc tgaaatagaa gcgcggccgt
     2461 cccagactcc ggaggatcac ctcggacatc ggattgacgt ccgcgatcgc ggcgctcgga
     2521 tcgacgctgt gcaccgccgc ccggatcccg gcgaccaccg ccgtcggcgt ggtgccccgc
     2581 acccggatca cgagcgggaa gttggtggtc atcgtggagc tctggagata ggtcgagtac
     2641 atctcgggct ggggatctga caccgggccg aaattccgga tgttcgagac cacacccacg
     2701 atggtcgcga acgtcgtatc ccccgtgtag aagcgcttcc cgacggcatc ccccccgtga
     2761 aagtcccgct tcaccagcgc ctggttgacc accaccaccg acggcgagcc cggccggtcg
     2821 tcgtcggtcg gcaggagcag tcgtccggcg agcaaccgct ggcccgtgac cgcgaagaag
     2881 tccggggata cgtcgcgctg ttcgacgagc ggcatgctgt tggggttgtc cggcggcatg
     2941 ccttcgtagc ggatgttcga gttgttcccc caattctggt agggcatcat gttgatggac
     3001 gcggcggctt cgactcccgg cacgttgcga atggcggtca acgccggttc gagcatcccc
     3061 cgcacgggat tggtcgtgtt gtacgcccgg gccgaggtcg tcaccgacat ggtgagaatg
     3121 cgcgaggcat cgaagcccgg atccttgtcg agcaatccca cgaaggcctt cagcaccaga
     3181 ccggcgccga ggagcagcac cagggagagg cacatctcga acaccaccag tccccgcagg
     3241 gcccggtggt gctcccgtcc cccacccgat cccctcccgg cgcgcaacgc gtcctgcggg
     3301 tcgacgcggg agccgcgcca ggccggtacc gcgccgcagg ccagggcgac gatgaacgcg
     3361 agtgccacgg agaatcccag gacccgaagg tcgacgtgca acccggagag ttggggcaaa
     3421 cgcgcggccg cgagggcgcc gatggtccgg acgccgagca cggccaggcc aacccctccg
     3481 accaacccga cggtcgcgat aacccccgcc tcgatcagcg cgggccgcat cgtctcccag
     3541 cgggtggcgc cgatggccgc acggatggcc gtctcccggc ggcgctgcac gccccgggcc
     3601 agcaggaggg cggcgacgtt gattaccgcg atgagcagga ccatgcagac ggcgccgaac
     3661 atgagcagca gcggtgtctt gatcgaagcc aggctttcgg tacggagtgg tgcgacgcgg
     3721 acggcatccc cgttgaggta gggataggtc gttacgaggt tctgaaagag cccccgcatc
     3781 tcggcatcgg cggtggccac ggtggctccc ggcgccagtc ggccgacgac cttgaggtaa
     3841 ttcatttgcc gggtcgtgag ctgcctcggc tcgaagcgca tgggcgccca gacgtcggcc
     3901 gacatgaatt gcccgccaat cggaatctgg aagtcgtggg gcaggacgcc gacgaccgtc
     3961 tgcggaaccc catcgatgat gacggaccgg ttgaccaccg acggatcgcc gcccaggtgg
     4021 tcccgccaga gccggtcgga aatcatgacc accgccggcg ccccggggcg gtcgtcgtca
     4081 ggtcccaaga cccggccgac ctgcgccgtc acccccagca tggggaagag gtggccggtg
     4141 gcgtacagct ccggcgcctg gaccgcgtct cccggcaagc cgacgagcgc cgtgccccag
     4201 gaaagcgcgg acaggtcctg gatctgcgtg gacgtgcggg cgaggtcgag gtaattggga
     4261 gcggagtgcg gccaggtacc caagggtccc g
//
LOCUS       DASUBP010000274        11883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_609137, whole genome shotgun sequence.
ACCESSION   DASUBP010000274 DASUBP010000000
VERSION     DASUBP010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 11883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11883
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_609137"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="VFU75_10240"
     CDS             <1..317
                     /locus_tag="VFU75_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467262.1"
                     /translation="RRPHRYGEGMAAPAAGRHAACAALASALLSHPKVTGSVVVSFTV
                     QGLQAGDPGLSAVIALKGPFADTLTATLPVKYRDTMAETVMMADVQALEQRLAQWMGG
                     AK"
     gene            314..1432
                     /locus_tag="VFU75_10245"
     CDS             314..1432
                     /locus_tag="VFU75_10245"
                     /inference="COORDINATES: protein motif:HMM:NF017181.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEU5467263.1"
                     /translation="MKTILVVTLVCAASTAHAQSTVAEADSVLARLIPTYGVSGSEAP
                     VREAVKSLLPGWAKPETDTAGNLWLHVGSGGSPVVYVAHMDEIGFQVTAINADGSLEL
                     DRTGGFFESLFEGMPALVHTPRGDIPGVIPPRDSLPRDPRELRHQPPLVRVEGDPNHS
                     TLRVDVGTTTRAATEALGVTVGSTVTMPKSYQTLAGTRATGRSFDDRVGDAALILAVR
                     HLDRAQLKHEVIFLWSTREETGLFGARAAARSLGTAPARVYAIDTFVSSDAPLDPKNF
                     ADQPLGQGPVVRALDNSSVTPPALVDSVLAIASARHIPIQVGTTNGGNDGSTFVPFGV
                     PDVAMGWPLRYSHSPAEVIDLKDVAALANIVQATAEAW"
     gene            1429..2967
                     /gene="purH"
                     /locus_tag="VFU75_10250"
     CDS             1429..2967
                     /gene="purH"
                     /locus_tag="VFU75_10250"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEU5467264.1"
                     /translation="MRALISVSDKRGIADFAKELVRLGWEIVSTGGTAEALRKEGVKV
                     ISIDEVTGFPEMMDGRVKTLHPKVHGGLLARRDNPGHLAAMKQHGITAIDLVAVNLYP
                     FRETVAKPSTTFEHAIENIDIGGPSMLRSAAKNHDAVIVVVDPADYTGIIAALKAGGV
                     PADERRRLATKVYAHTSAYDAAITNYLATRAGEPFPPSVALVLERQQSLRYGENPHQP
                     AALYVTDEARGMRDMVQLHGKELSFNNLLDVDAAVSSVLPWDSEHPACAIIKHTTPCG
                     LALGGSAAEAYQRALATDRQSAFGSVIAYNVTVDRAAAEAMRDLFVEVVVAPAVADDA
                     LAVFREKKNIRVITLPRPDASPTWDYKRVRGGFLVQERFRPGAPDAEKAWTVPTKRKP
                     TDAEWRDLRFAWAAVGVVKSNAILLARGEAAIGIGAGQMSRVDSSFLAVHKARQQGHD
                     PKGSVLASDAFFPFRDGMDEAAAAGVRAVIQPGGSVRDPEVIGAADEHGIAMVLTGTR
                     QFRH"
     gene            2985..5117
                     /locus_tag="VFU75_10255"
     CDS             2985..5117
                     /locus_tag="VFU75_10255"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEU5467265.1"
                     /translation="MNDRDARPPYGIGALTALVVFGLYLLTLAPTVQYWDAPEYMAAA
                     HGFGIPHPPGNPLFVLLAHVWGLLPLAADYGKRINLFAAFTSACSAGLWFMITERWLQ
                     PIVPVRVVRCIAAFTGTIAGAAAFTVWNQSVVNEKVYTVSLLTITLVLWLAIRWSDQG
                     PGARRDNFIVLIVYLLILTTANHQMGLLAGPAVLALVLMVDWRVMFSPRVLVASAVAV
                     LVALTVYLILPLRAMHHPYMNEGDPQTWQALSDVLGRAQYGKPSVTDRQATFVAQIGM
                     WLQYFTWQWGRDLTDRAQGLFAIGFGVLGLLGAVRHWMAERRQAIAMITLMFTVTIAL
                     IFYLNFKYGYSEFPGQQLIREVRERDYFFIVSYSAWGVWVAMGLATLMEWFQEGLQAR
                     VPAAPQRWLLVSPVAALALIPLWTNHLSAPRSGETMARDFAHDLLNSLEPYAVIITGG
                     DNDTFPLWYAQEVDHVRPDVTVIVTSLGNINWYLEQAQNRPLPTYDAAAGPAFLRNRT
                     WTKPGPWFTKLYTTPSDTLPEVESVPQPVSGKLGPLAITLDPRSLPNPGYLTRIDLAV
                     YEMIKEDLGKRPIYFSTTTADYGDRLGLSPYLVTEGMVRHLMTTPVVANDSIKMSPVQ
                     GRLTNIPLTVTLAFDVYHGTSAARYRPKGWVDGASQNILVPYIITYDTVAEALAKTDQ
                     KRAQQAYDTAVAILRNTRYQFQLAPPTP"
     gene            5157..7493
                     /locus_tag="VFU75_10260"
     CDS             5157..7493
                     /locus_tag="VFU75_10260"
                     /inference="COORDINATES: protein motif:HMM:NF022481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HEU5467266.1"
                     /translation="MDETTATATTTTDAQRPPYLMALGVVVGTLVLYILTLAPTTQFW
                     DTSEYIAAAKVLGIPHPPGNPLFTMMAHVWGFFPLMAGYAARINLFAATTSAVAAGCW
                     FLIGERWLRDIVPLQLPRRLCALAGAIVAATAFTVWNQSVVNEKVYTLSLLSIALILW
                     LIVRWDDQPAGEAHDHHLLAIVYLLALTSTNHMMGVLVGPVVVVLLFPPLMTNRAPTD
                     AGRNAEWGQWFAFSAVFTVIVCTGLESMGMVIAGLAIFALAFVFTLYTKNWRFALVAL
                     AVTAIGLSVYVFLPIRAAHFPPINEGEPTTWPALRDVLTRAQYGKPAVTERQASFGAQ
                     LGMWVQYFTWQWGHDLRQSYEAALAVVFGVLGVLGAIRHWYANRRHAIAMTVLMLTLT
                     ILLISYLNFKYGFSWDAAKPTCGPVPTGDCIPREVRERDYFFICSFALWGIWVGMGLA
                     TVMEWLQDGLKVREPSEARRWAFASPVLLIAVVPLFANRLTASRHGETLARDFAVDLL
                     QSVEPYGVLVTAGDNDTFPLWYAQEVEHVRQDVTVLNLSLGNTDWYLRQMQRRPLADF
                     DTTNALPIYKGHTWIKPSGPPIAATPEQVDAIPPVYPVQQRSMLHFGTIPIIVDPQYL
                     GKDYLERADIVTLMVIRDQMGKRPIYFSRTTGYYPDQFGLSAYLEGQGFARKLMPAPI
                     VASDSVVLTQDMGYVNLPRSTALLTELYHHATTTRPRPRGWVDRPSEGILYTYGVMDQ
                     SLANVLQKKNPALAARLSAMADSVFRNTSIGSSPTALR"
     gene            7529..7612
                     /locus_tag="VFU75_10265"
     tRNA            7529..7612
                     /locus_tag="VFU75_10265"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7563..7565,aa:Ser,seq:tga)
     gene            7632..8165
                     /locus_tag="VFU75_10270"
     CDS             7632..8165
                     /locus_tag="VFU75_10270"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HEU5467267.1"
                     /translation="MAKLVYGLNQSLDGYVDHTKLGPPPPAAFRHFIELVRGAAGLIY
                     GRRTYEIMRYWDEDLPEWDAGDHEFAAAFRSQPKWVVSRSLKTAGPNATIVADDFAKV
                     IRKLKTERSGEILVGGPVLAHSLTEAGLIDEYRLYLHPVVLGGGAPFFAGPRPPLHLV
                     ASDRIAGDLIRLTYVPA"
     gene            8353..8670
                     /locus_tag="VFU75_10275"
     CDS             8353..8670
                     /locus_tag="VFU75_10275"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467268.1"
                     /translation="MRKLMQSCFLAVAAALLAGALACVPPPPPGAVVVERRPPPERVE
                     IIGVAPAPGYIWIAGHWQWSGGDFMWVGGRWAPIERGYRTWEPGHWRHHGHEWYWVEG
                     HWR"
     gene            8833..9411
                     /locus_tag="VFU75_10280"
     CDS             8833..9411
                     /locus_tag="VFU75_10280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467269.1"
                     /translation="MCAIGLLPAVLPAQATGTTTFNAPYRAFSNSEFGVLVSFPDGGG
                     TAFEGAYRVSSGKFDIGFKGGLFSPGGGGSTALLIGVEGRERVLSHNEDFPLDGALIV
                     GAGASLVSGGSALIVPVGLSLGRRIDLQDSPVSIVPYVQPTAFFIADGGGHVDFDLGL
                     GADFRLTRRFDARLSAGIGDIQGISIGAIWVH"
     gene            complement(9466..10206)
                     /locus_tag="VFU75_10285"
     CDS             complement(9466..10206)
                     /locus_tag="VFU75_10285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467270.1"
                     /translation="MLRHVIAVTLAGVPVVTIAAQSPRVANPERPTYATHAYGVAPGY
                     VEIEQGVSATGISSLAEGTVWSMALKLGITPSLQAEFFGPLYARTPSGGGVGDLGLSL
                     KFRTDLSEKSALALIPAATAPTGSVAKGLGAGRALGSLTAVYSVDLPAAVHVDLNAGP
                     TGLGAGATQWFFSASGSWGGSTIGVMAECFDFTPGAAGPRLAGLLGALTVRVAPWAVM
                     DAGGQWGTTRSSPNALFLGVTTNLGKAF"
     gene            10273..10497
                     /locus_tag="VFU75_10290"
     CDS             10273..10497
                     /locus_tag="VFU75_10290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467271.1"
                     /translation="MESKHAAPTERRRRPFDRRALFPRRKSAERRVGDRRSAKSVATN
                     GERRVASSRRGEERRVGKERRSWAERRHAH"
     gene            complement(10510..10977)
                     /locus_tag="VFU75_10295"
     CDS             complement(10510..10977)
                     /locus_tag="VFU75_10295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467272.1"
                     /translation="MSQAVRWLLRAGVGGIAVAAVLSCKNVDEPPAPAAIAGSWQYTE
                     TLADNLFQITCADTGVYTFNQDGPKFSGNYVQSGICQNQGTKFFNTGHGPVTDGSVTN
                     VRLQFTASDLCSYTGQLSAAHDAVNAGTGICDFVDSSTSRHYSMQITWQMTRQ"
     gene            11034..11393
                     /locus_tag="VFU75_10300"
     CDS             11034..11393
                     /locus_tag="VFU75_10300"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU5467273.1"
                     /translation="MSRTIFLLAVIAVSGLSCSDPFSIPTTLVIINDNYQFVPDTVTI
                     THGTTIEWKNESSQYHLIQADSVAPSGLQEMEFAPVGGVTREYPQTQWTLTMGGPGVY
                     PYHCVLHTQMHGVLIVR"
     gene            11468..11558
                     /locus_tag="VFU75_10305"
     tRNA            11468..11558
                     /locus_tag="VFU75_10305"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11502..11504,aa:Ser,seq:gct)
     gene            11638..>11883
                     /locus_tag="VFU75_10310"
     CDS             11638..>11883
                     /locus_tag="VFU75_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012951.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HEU5467274.1"
                     /translation="MTTRVRFAPSPTGYLHVGGARTALFNWLYARHVGGQFLLRIEDT
                     DKQRSTDEHTQVILDGLTWLGLDWDEKPIFQGARIKRH"
BASE COUNT         1939 a         4079 c         3809 g         2056 t
ORIGIN      
        1 cccggcgacc ccaccggtat ggtgaaggga tggcggctcc ggctgccggc cgccatgcgg
       61 cctgcgccgc gctcgcctcc gccctgctct ctcaccccaa ggtcactgga tcggtcgtcg
      121 tcagcttcac cgtccagggt ctgcaggccg gagaccccgg cctgagcgcc gtcatcgctc
      181 tcaagggccc gttcgcggat accctgaccg cgacattacc ggtgaagtac cgcgacacca
      241 tggcggaaac ggtgatgatg gccgacgtcc aggcgctcga acagcggctg gcgcagtgga
      301 tgggaggcgc caagtgaaga ccatcctcgt ggtcacgctg gtgtgcgccg catcgacggc
      361 gcacgcgcag tccacggtgg cggaagcgga ttccgtcctg gcgcgactga tccccacgta
      421 cggcgtctcg ggaagcgaag ccccggtgcg cgaggccgtg aagtccctgc tccccggctg
      481 ggcgaaaccg gagaccgaca ccgccggcaa cttgtggctg cacgtgggat ctggcggctc
      541 gcccgtcgtc tacgtcgcgc atatggacga gatcggcttc caggtcacgg ccatcaacgc
      601 cgatggctca ctggagctcg accgaaccgg cggctttttc gaatcgctct tcgagggcat
      661 gccggcgctg gtccacacgc cgcgcgggga cattcccggc gtcattccgc cccgcgattc
      721 cctcccccgc gacccccgtg agctgcggca ccagcctccg ctggtccgcg tggaggggga
      781 ccccaaccac tcgacgcttc gcgtggacgt cggcaccacc acccgcgccg ccaccgaagc
      841 gctcggcgtc accgtcggca gcaccgtcac gatgccgaag agctaccaga cgctcgccgg
      901 cacgcgcgcc acgggccggt cattcgatga tcgggtcggt gatgcggcgc tgatcctcgc
      961 ggtgcgccac ctcgaccggg cacagctcaa gcatgaagtc atcttcctct ggagcactcg
     1021 tgaagagacg ggactgttcg gtgccagagc cgccgcgcgt tcgctcggta ccgcgccggc
     1081 acgggtctat gccatcgata cgttcgtctc gtcggatgct cctctcgacc ccaagaactt
     1141 cgccgaccag cctctgggcc agggcccggt cgtgcgggca ctcgacaaca gctcggtcac
     1201 cccgccggcc ctcgtcgact cggtcctcgc gatcgcgtcc gcgcgccaca tcccgatcca
     1261 ggtcggcacc accaacggcg gcaacgacgg ctcgaccttc gtgccgttcg gcgtgccgga
     1321 cgtcgccatg gggtggccgc tgcgatattc gcactcccct gccgaagtga tcgacttgaa
     1381 ggacgtcgcc gctttggcga acatcgtgca ggccaccgcg gaggcgtggt gagagcgttg
     1441 atctcggttt cagacaagcg gggcatcgcg gacttcgcga aggagctcgt gcggctcggc
     1501 tgggagatcg tttccaccgg cgggaccgcg gaggcgctgc ggaaggaagg cgtgaaggtg
     1561 atctcgatcg acgaggtcac cggcttcccg gaaatgatgg atggacgggt gaagactctc
     1621 catccgaaag tccacggcgg cctgctcgcc cggcgcgaca atcctgggca cctcgcggcg
     1681 atgaagcagc acggcatcac ggcgatcgac ctcgtggccg tgaatctcta ccccttccgc
     1741 gaaaccgtcg ccaagccgag cacgaccttc gagcacgcca tcgagaacat cgacatcggc
     1801 ggcccctcga tgctccgctc ggcggccaag aaccacgacg cggtgatcgt cgtggtggat
     1861 cctgccgact acaccgggat catcgccgcc ctcaaggccg gcggcgtgcc cgcggacgag
     1921 cggcgccgcc tggccaccaa ggtctacgcc catacgtcgg cctacgacgc ggcgatcacg
     1981 aactatctcg cgacccgcgc cggtgagccc tttccaccct ccgtggcgct ggtgctcgag
     2041 cggcagcagt cattgcggta tggggagaat ccgcatcagc cggcggccct ctacgtgacg
     2101 gacgaggcgc gcgggatgcg ggacatggtt cagctccatg gcaaggagct gtcgttcaat
     2161 aatctcctgg acgtcgacgc ggcggtcagc tcggtcctgc cgtgggactc cgagcatccc
     2221 gcctgcgcca tcatcaagca caccactccc tgcgggctgg cgctcggcgg gtccgccgcg
     2281 gaggcttatc agcgcgcgct ggccaccgac cgccaatcag cattcggctc cgtgatcgcg
     2341 tacaacgtca cggtggaccg ggccgccgcc gaggcgatgc gggacctgtt cgtggaggtg
     2401 gtggtggcgc cggcggtggc ggatgacgcc ctggcggtgt tccgggagaa aaagaacatc
     2461 cgcgtgatta ccttgccccg gcccgacgcc tcgcctacct gggactacaa gcgcgtgcgg
     2521 ggcggattcc tggtccagga acgcttccgg cccggcgcgc ccgacgccga gaaggcgtgg
     2581 acggtgccga ccaagcgcaa gccgaccgac gcggagtggc gggatttacg cttcgcctgg
     2641 gcggccgtcg gtgtagtaaa atccaacgcg attctcctgg cccggggcga ggccgccatc
     2701 ggcatcggcg cgggccagat gtcgcgtgtg gacagctcgt tcctggcggt ccacaaagcc
     2761 cggcaacaag ggcatgatcc gaaagggagc gtcctcgcga gcgacgcgtt cttccccttc
     2821 cgggacggca tggacgaggc ggccgccgcc ggcgtgcggg cggtcatcca gcccggcgga
     2881 tcggtgcggg atcctgaagt catcggggcc gcggacgagc acggcatcgc gatggtgctg
     2941 accggtaccc ggcagttccg acactaagcg gagactctga gcctatgaac gaccgcgacg
     3001 ccaggccccc ctacggcatt ggcgccctca cagccctcgt ggtgttcggg ctgtatctgc
     3061 tgacgctggc gcccacggtg cagtactggg acgcccccga gtacatggcg gccgcgcacg
     3121 gcttcgggat tccgcacccc ccggggaacc cgctcttcgt actcctcgcg cacgtgtggg
     3181 gattgctccc cctcgccgcc gactacggca agcggatcaa cctcttcgcc gcattcacca
     3241 gtgcctgctc cgcggggctc tggttcatga tcaccgagcg gtggctccag cccatcgtgc
     3301 ccgtccgcgt cgtgcggtgc atcgccgcct ttaccggcac catcgccggc gccgcggcct
     3361 tcaccgtgtg gaaccagtcc gtcgtgaacg agaaggtcta cacggtctcc ctcctcacga
     3421 tcacgctggt gctctggctg gcgatccgct ggtccgatca gggtcccggc gcgcgtcgcg
     3481 acaatttcat cgtgctgatc gtttatctcc tgatcctgac gaccgcgaac caccagatgg
     3541 gcctgctggc gggtcccgcg gtgctcgcgc tggtgctgat ggtcgactgg cgggtgatgt
     3601 tcagccctcg cgtgctcgtc gcctccgcgg tggcggtgct cgtcgccttg accgtgtacc
     3661 tgatcctgcc cctgcgggcc atgcaccacc cctacatgaa tgaaggggac ccccagacct
     3721 ggcaggcgct ctccgacgtg ctcggccgcg cgcagtacgg caagccatcg gtgaccgacc
     3781 ggcaggcgac gtttgtcgcg caaatcggca tgtggctcca gtacttcacg tggcagtggg
     3841 gccgcgacct gaccgaccgc gcgcagggcc tgttcgcgat cggcttcggc gtgctcggtc
     3901 tcctcggcgc cgtgcgccac tggatggcgg aacggcggca ggcgatcgcg atgatcaccc
     3961 tcatgttcac ggtgaccatc gctctcatct tctatctcaa cttcaagtac gggtactccg
     4021 agttcccggg ccagcagctg atccgtgagg tgcgcgagcg ggattacttc ttcatcgtgt
     4081 cgtactcggc ctggggcgtg tgggtcgcga tgggtctggc gactctcatg gagtggttcc
     4141 aggagggcct gcaagcccgt gtgcccgccg ccccgcagcg gtggctgctc gtgtcaccgg
     4201 tggcggcgct cgcgctcatt cctctgtgga ccaatcacct gagcgcgccc cgcagcggtg
     4261 agacgatggc ccgggacttc gctcacgatc tcttgaactc gctcgaaccg tacgcggtga
     4321 tcatcaccgg cggcgacaac gacacgttcc cgctctggta cgcgcaggaa gtcgatcacg
     4381 tccgcccgga cgtcacggtc atcgtgacct ccctcggcaa catcaactgg tacctcgagc
     4441 aggcccagaa ccggccactg ccgacctacg acgcggcggc gggtccggca ttcctccgca
     4501 accggacgtg gaccaagccg ggcccctggt tcaccaagct ctacacgacg cccagcgaca
     4561 cgttgccgga agtggaaagc gtcccgcagc ccgtgagcgg aaagctggga ccgctcgcca
     4621 tcaccctcga tccgcggtca ctccccaatc cgggctattt gacccgcatc gatctcgcgg
     4681 tctacgaaat gatcaaggaa gacctcggca agaggccgat ctatttctcc accacgaccg
     4741 ccgactacgg agaccggctg ggactgtcgc cctatctggt gaccgagggc atggtccgtc
     4801 acctgatgac gacgccggtg gtggcgaacg acagcatcaa aatgagcccg gtgcagggcc
     4861 gcttgaccaa cattcccctc accgtcacgc tggccttcga cgtctatcat ggcaccagtg
     4921 ccgcccggta ccgtcccaag ggctgggtgg acggagcatc ccagaacatc ctggtcccgt
     4981 acatcatcac ctacgacacc gtcgccgagg cccttgcgaa gaccgatcag aaacgggccc
     5041 agcaggcgta cgacacggcg gtcgccatcc tccggaatac gcggtaccag ttccagctgg
     5101 caccgccgac accctagggg atgattagct tacaagcctc ttcaacctga ccggcaatgg
     5161 acgagacgac cgcgacggcc acgaccacga ctgacgcgca gcgcccgccg tacctgatgg
     5221 ccctgggtgt ggtcgtcggt acgctggtgc tctacatcct gacgctcgcc cccacgacgc
     5281 agttctggga cacgtcggaa tacatcgccg ccgcgaaggt gctgggcatt ccccacccgc
     5341 ccggcaatcc cctcttcacc atgatggcgc acgtctgggg cttcttcccg ctgatggcgg
     5401 gatatgcggc gcgcatcaac ctcttcgcgg cgacgacgag cgcggtggcg gcggggtgct
     5461 ggttcctgat cggcgagcgc tggctccggg acatcgtccc gctgcagctg ccccggcgcc
     5521 tctgcgccct cgcgggcgcg atcgtggcgg cgaccgcgtt caccgtgtgg aaccagtcgg
     5581 tggtgaacga gaaggtctac acgctttcgc tcctctcgat cgcgctgatt ctctggctga
     5641 tcgtccgctg ggatgaccaa cccgcgggcg aggcgcacga tcaccacctg ctcgcgatcg
     5701 tgtacctgct cgcgctgacc tccaccaacc acatgatggg tgtgctcgtg ggcccggtgg
     5761 tggtggtgct cctgttcccc cccctcatga ccaaccgcgc gcccaccgat gccggccgca
     5821 acgccgaatg ggggcagtgg ttcgccttct cggcggtgtt cacggtgatc gtctgcaccg
     5881 ggctcgagag catggggatg gtcatcgcgg ggctcgcgat cttcgccctc gccttcgtct
     5941 tcacgctcta taccaagaac tggcgctttg ccctggtcgc gttggccgtc acggccatcg
     6001 gcctgtccgt ctacgtgttc ctgcccattc gcgccgcgca cttccctccc atcaacgagg
     6061 gggaaccgac cacgtggccg gcgctgaggg atgtcctgac ccgcgcgcag tacggcaaac
     6121 cggcggtcac cgagcggcag gcgagcttcg gcgcccagct cggcatgtgg gtgcagtact
     6181 tcacctggca gtggggacat gacctgcggc agagctacga ggccgccctc gccgtggtgt
     6241 tcggcgtcct gggcgtcctg ggcgcgatcc gccattggta cgccaatcgc cggcacgcga
     6301 tcgcgatgac ggtcctcatg ctcacgctga ccatcctcct gatctcgtac ctcaacttca
     6361 agtacggatt ctcgtgggac gctgcgaaac ccacgtgcgg tcccgtgccg accggggact
     6421 gcatcccccg cgaggttcgc gagcgggact acttcttcat ctgttcgttc gcgctctggg
     6481 gcatctgggt gggcatggga ctcgccaccg tcatggagtg gctgcaggac gggctcaagg
     6541 tgcgggagcc gagcgaggcc cgccgctggg cgttcgcgag tccggttctg ctgatcgcgg
     6601 tggtgcccct cttcgccaac cgcctgaccg cttcccggca cggcgagacc ctcgcgcgtg
     6661 acttcgcggt ggatctcctg cagtcggtcg agccgtacgg cgtgctcgtg acggccggtg
     6721 acaacgacac cttccccttg tggtacgccc aggaggtcga gcacgtccgt caggatgtga
     6781 ccgtgctcaa cctgtcgctc ggcaacaccg actggtacct gcggcagatg cagcggcggc
     6841 cgttggccga cttcgacacc accaacgccc tgccgatcta caagggacac acgtggatca
     6901 agccgtcggg cccgccgatc gccgcgacgc cggagcaggt cgacgccatt ccgcccgtgt
     6961 atccggtgca gcagcggtcc atgctgcact tcggaaccat tcccatcatc gtggatcccc
     7021 agtacctcgg gaaggactac ctggagcgcg cggacatcgt cacgctcatg gtgatccgcg
     7081 atcagatggg caagcggccg atctacttct cgcggacgac cggttattac cctgaccagt
     7141 tcggcctgtc cgcctacctc gagggtcagg gctttgcgcg gaagctcatg ccggcgccga
     7201 tcgtggcgag cgacagcgtg gtgctgacgc aggacatggg gtacgtgaac ctcccccgct
     7261 ccacggccct gctcacggag ctctatcacc acgcgaccac gacgcggccg cggccgcgcg
     7321 gctgggtgga ccgcccgtcg gaggggattc tttatacgta cggcgtgatg gatcagagcc
     7381 tcgcgaacgt cctgcagaag aagaacccgg cgctcgcggc cagactctcg gccatggcgg
     7441 actcggtatt ccggaatacc tcgatcggga gcagccccac cgcgctccgt tgacgggagg
     7501 gggctctgcc ccctaggatg acgattcggg agggctagac ctaattggta aggcggcggt
     7561 cttgaaaacc gctgggcgca agcccttaca ggttcgagtc ctgtgccctc cgtgaaacgg
     7621 agattagcag catggcaaaa ttggtttacg gactgaacca gtccctcgac ggctacgtcg
     7681 accacacgaa gctggggccg cctccacccg cggccttccg gcacttcatc gagctggtgc
     7741 gtggcgcggc ggggctcatc tacggtcgcc gcacgtacga aatcatgcgc tactgggacg
     7801 aagatctccc cgagtgggac gcgggggacc acgaattcgc ggcggccttc cggagccagc
     7861 cgaagtgggt cgtgtcgcgt tccctcaaga cggccggccc caacgccacg attgtcgcgg
     7921 atgacttcgc gaaggtgatc cgcaagctga agaccgagcg gtccggcgag attctcgtcg
     7981 gcggaccggt cctggcgcac agcctgaccg aggccgggct catcgatgag tatcgcctct
     8041 atcttcaccc cgtcgtgctg ggcggtggcg cgccattctt cgccggcccc cggccgcccc
     8101 tgcacctggt ggccagcgat cgaatcgccg gtgacttgat ccggttgacg tacgtgcctg
     8161 cctagacgcc tctgctcgac gcttctgctc gacgtttctc ctcgacgctt ctccttgacg
     8221 cttctcctcg acgcctttga gtagttggtg accctgatca cccccgcgca ggtctctcat
     8281 cggcttaacg caggaacggt tctcccgtct gtcgggtaca cggactcgac tcctacttcc
     8341 ggagccctga cgatgagaaa gctcatgcag tcgtgtttcc tcgcagttgc agcagcattg
     8401 ctcgccggag cgctggcgtg tgtgccgcct ccgcccccgg gagccgtggt cgtcgaacgg
     8461 cggccaccgc ccgaacgggt cgagatcatc ggcgtagcgc cggcaccagg gtacatctgg
     8521 atcgccggtc actggcagtg gagtggtggt gacttcatgt gggtcggcgg ccgctgggcc
     8581 ccgattgagc gcggctaccg aacgtgggag cccggtcact ggcgccatca tggccacgag
     8641 tggtattggg tcgaaggcca ctggcggtag cgcgtcaccc acgcaacacc aagtgacagg
     8701 aatcacagag aggggggtcg gggaataccg gaacgcgcgc gcgagcctcg ggtacccgtt
     8761 gtcggagatt atccaacttc gacaggagcg atgatgtcgt ccaaacgaac gacactttgg
     8821 gcattcgcgg tcatgtgtgc gatcggtctt cttccggccg ttctgcccgc acaagccact
     8881 ggtaccacca cgttcaacgc gccctaccgg gcattctcga actcggagtt cggcgttctc
     8941 gtgagcttcc ccgatggcgg cggcaccgct ttcgaaggcg cctaccgcgt atcctccggc
     9001 aagttcgaca tcggcttcaa aggcggactc ttctcccccg gtggcggcgg cagtaccgcc
     9061 ctgctcatcg gcgtcgaggg ccgcgagcgc gtgctctcgc acaatgaaga cttcccgctg
     9121 gatggcgcgc tgatcgtagg tgcgggcgcg agcctggttt ccggcggatc agccctcatc
     9181 gtccccgtcg gcctctccct cggccgccgg atcgacctgc aggactcgcc ggtcagcatc
     9241 gtgccgtacg tccagccgac ggcgttcttc atcgcggacg gcggtggcca cgtcgacttc
     9301 gacctcggac tgggagccga cttccggctc accaggcgct ttgatgcgcg gctctccgcc
     9361 ggcatcgggg acatccaggg gatttcgatc ggtgcgatct gggtccacta gtcgtcatcc
     9421 tgagcgccgc gcgcggccgc gcgcggcgct caggaagcca cgggactaga aagccttccc
     9481 gaggttcgtc gtcactccca ggaatagcgc gttcggtgag ctccgcgtcg tgccccactg
     9541 ccccccggca tccatcacgg cccaaggcgc aacccgcacc gtcagggccc cgagcagtcc
     9601 ggcgaggcgt ggtccggcgg caccgggcgt gaagtcgaag cactccgcca tcacgccgat
     9661 ggtactccca ccccacgatc cgctcgcgga gaagaaccat tgcgtggcgc cggcaccgag
     9721 gcccgtgggg ccggcgttga gatcgacgtg caccgctgcg ggaagatcga cgctgtagac
     9781 ggcggtaagt gagccgaggg cccggcccgc gccgagtccc ttggcgacgc tccccgtggg
     9841 ggcggtggcc gccgggatga gcgcgagcgc cgacttctcc gacaagtcgg tccggaactt
     9901 cagtgagagc cccaggtcgc ccacgccgcc accgctgggt gtgcgagcgt agagggggcc
     9961 aaagaattca gcctgaagcg agggtgtgat gcccaacttc aatgccatgc tccagacggt
    10021 cccctcggcg agggacgaaa tccccgtggc actgaccccc tgctcgatct ccacgtaacc
    10081 gggggcaact ccatatgcgt gagtggcgta ggtcggacgc tccggattcg ccacacgtgg
    10141 cgattgagcg gcgatcgtga ctacggggac gccggccagc gtgaccgcga tcacatgtcg
    10201 aagcatggtt cgaaagagta acgggcccgg gcctctagcg ggagcggaaa cggggcccca
    10261 ctttttgcct ccatggagag caagcacgca gcaccgaccg agcgtcgccg ccgtccgttc
    10321 gatcgccggg ccctgtttcc acgccgcaag agcgccgagc gccgggtcgg agaccggcgg
    10381 tccgcgaaat cggtggcgac gaatggggaa cggcgggtgg ccagtagccg gcggggcgag
    10441 gagcggcggg tcgggaaaga gcggcgaagc tgggcggagc gacggcacgc gcactgacgc
    10501 gcgcgggtat tactgccggg tcatctgcca ggtgatctgc atggagtagt gccgcgacgt
    10561 cgacgagtcc acaaagtcgc agatccccgt ccccgcattc accgcatcat gcgcagccga
    10621 caattggccg gtgtagctgc agagatcact cgccgtgaac tgcagccgga cgttggtcac
    10681 cgagccgtcg gtgacgggcc catgtccggt gttgaagaac ttggtcccct gattctggca
    10741 gattcccgac tgtacgtagt tccccgagaa cttcggccca tcctgattga acgtgtagac
    10801 acccgtatcg gcacaggtga tctggaacag attgtcggcc agggtttcgg tgtactgcca
    10861 ggaaccggcg atggccgcgg gcgccggcgg ttcgtcgacg ttcttgcagg agaggacggc
    10921 tgcgacggct atccccccga ccccggcacg gagcaaccac ctgacggcct gagacattcc
    10981 actatgctac ggctcttgat gccggcgtca agtttgggta ggttggcaat tgtatgagtc
    11041 ggacaatctt cttactcgcc gtcatagccg tttccggcct ctcctgcagt gatccattca
    11101 gtatcccgac gaccctggtg atcatcaacg acaactacca gttcgtgccc gacaccgtga
    11161 cgatcacaca cgggacgacc atcgagtgga agaacgagag ctcccagtat cacctgatcc
    11221 aggcggacag cgtcgccccc tctgggttgc aggaaatgga gttcgcgccg gtgggcggcg
    11281 taactcgtga atatccccag acgcagtgga ccctcacgat gggcggtccc ggcgtctatc
    11341 cctaccactg cgtacttcat acgcagatgc acggagtcct gatcgttcgc tgaagtactg
    11401 gtcccctgcg tcttgtgtct ccccacaccc ccggctaaac tgctcccctc gttgatgaag
    11461 tctgtcggga ggggtggccg agtggctgaa ggcggcggtt tgctaaaccg ttatagggcc
    11521 taatagccct atcgggggtt cgaatccccc ctcctccgtg aactgcaggg atcagggacc
    11581 aggtgccagg gatcaggcac ggcgaccctg acccccgacc cctgacccct gacctccttg
    11641 actactcgcg tccgcttcgc cccatcgccg accggatacc ttcacgtcgg cggcgcccgt
    11701 accgccctct tcaattggct ctacgcccgc catgtcggcg gacagttcct gctgcgcatc
    11761 gaagacaccg acaagcagcg ctccaccgac gagcacaccc aggtcatcct cgacggcctc
    11821 acctggctcg gcctcgactg ggacgagaag cccatcttcc agggcgcgcg catcaagcgg
    11881 cac
//
LOCUS       DASUBP010000275         4034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1031815, whole genome shotgun sequence.
ACCESSION   DASUBP010000275 DASUBP010000000
VERSION     DASUBP010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4034
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1031815"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="VFU75_10315"
     CDS             <1..271
                     /locus_tag="VFU75_10315"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pirin-like C-terminal cupin domain-containing
                     protein"
                     /protein_id="HEU5467275.1"
                     /translation="PRRLYYLGTQRTTIALKSERGGRLLLIGGPPFPEPILMWWNFVA
                     RTPDEIAAARADWESHRRFGDVTAYRGPRLEAPTLSRFARANPVS"
     gene            complement(280..3159)
                     /locus_tag="VFU75_10320"
     CDS             complement(280..3159)
                     /locus_tag="VFU75_10320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467276.1"
                     /translation="MPSFAPQRRAAAAVLLGLAVVTCTRDLDAPRIPARIELAPRFVT
                     GATPPGNALAVDQVRAQVVRHSTAEVVVDKTYPFTSGTSLQLSISFSLTQPTDTFDVF
                     LDYERGTEILFSGSQSVVVTGGVTPAPVPLPVGYVGPGKGATSMTIGPRDTVLTSGDP
                     LQLRLTVLDTATPDSLYVGWGTNSGSVAVDPAGRLVASITRGTVTVRAISPTPPGLSD
                     STTVRIVPKPAAMVKVSGDGQTGVASQPLPLPLVVQVNGSDNLGVPGVTVTFAALSGG
                     AVDSAQVVTDSLGRASTGVTLGPSTAAQSFSATAGALSVTFGATGSAAPPKTWTGAVS
                     SDWGTAGNWNPAGVPTATDSVIVPPGTANSAVIRATAYSVAGLSIQSGAIVTMDTASL
                     LVNGSFTQAGTFTDPSVSSALSLNGTGKTLAGSIPILATTVIGTYAVTGNVLASTLQV
                     TGGLTLGGHTLTTTGAFGTAGTGVITMTNAADSLVVQGTAAFAGGVETGHLTAGTFVL
                     AGNFAVTVHSGFDAGPSHLTVFNGSATQSVAVFDTTHSNQFGSVEFRNKVGVVFDQLT
                     GVNGNARLDTSAVVTSTDTTYVGGGMALNGGLTTFPASLLRVGELWVNTTFNAGGTYA
                     VTATIFTGNGQTVPTGVTFPFLYCNGANVSVGSGVTVGTQFAVQAGTTTLSGDFSVPA
                     NTFIQFTGKLVLNGHAFSTGGDLSITQSALLQMTNAADSLYVFGNAFFEGGNESGLMT
                     AGLSLFFNNFTEGSGDPQAYAPSGSHIDVFVGGGTQTISFGHPGGAGASHFQGFAVDN
                     PQGALHIASDVYLMGGYAYVAGVPKIVHGGGQVVHYASLNVTGVTFDNVAIAYDAALG
                     GNNLIALDSVTFENYNVNSATPLIDITSPGNTASPGTFFLFNNMTFNTDITGAGGSGT
                     YLRVTDSNAGDGNVLTIDINSGLVVTEGMAHTILAGGAVVNWAQP"
     gene            3243..>4034
                     /locus_tag="VFU75_10325"
     CDS             3243..>4034
                     /locus_tag="VFU75_10325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467277.1"
                     /translation="MRIAVLSLAFLSATAMSLSSQTRPAPSVDPTVTAQGPRPLPGPV
                     YETPGFTRAVANGTRTRTGAPGPKYWVQHPRYQIAATLDPIQNRVTGRETVVYVNHAP
                     DALDRIAVHLRQNVFAPGVPRRQPVPITGGVTLSRVALDGRALVLEPRPAAVPITAPP
                     EQPPQPGQYVVDGTVMWIPLGTPLKSGDSLRLEVNWSYSPAPAPADGREGHDGHVYFQ
                     GYWYPEIAVYDDVDGWVADPYLLEAELYMDHADYDVRLTVPRGWVV"
BASE COUNT          686 a         1374 c         1321 g          653 t
ORIGIN      
        1 gccgcggcgc ctctactatc tcggcactca gcggacgacc atcgccctga aaagcgaaag
       61 gggaggccgc ctgctcctga tcggcggccc cccgtttccg gaacccatcc tgatgtggtg
      121 gaacttcgtc gcccgtacgc cggacgagat cgcggccgct cgggcggact gggaatcaca
      181 ccggcgcttc ggagacgtca ccgcctaccg cggcccccgg ctcgaagccc ccaccctgtc
      241 ccgcttcgcg cgtgcgaacc cggtcagctg aacccgcggc tacggctgag cccagttgac
      301 cacggcgccg ccggccagga tggtgtgcgc catcccctcc gtaaccacca atccgctgtt
      361 tatgtcgatg gtcaggacgt tcccatcacc ggcgttcgag tcggtcaccc gcaaataggt
      421 gcccgatcct ccagcacccg tgatgtccgt gttgaacgtc atgttgttaa agaggaaaaa
      481 cgtccctgga ctcgcagtgt tgcccggact ggtgatatcg atcaagggcg tcgccgagtt
      541 cacgttgtag ttctcgaacg tcaccgagtc gagggcgatc aggttgttcc cgccgagtgc
      601 cgcgtcgtag gcgatcgcca cattgtcgaa cgtcacgccc gtaacgttca ggctggcata
      661 gtgaaccacc tgtccgcccc catgcacgat cttcggcacc ccggccacgt aggcgtaccc
      721 gcccatgaga tagacgtccg aggcgatgtg cagcgcaccc tggggattgt cgaccgcaaa
      781 cccctggaag tgagaggcgc cggcgccgcc cgggtgaccg aacgagatgg tctgcgtccc
      841 gccacccacg aacacgtcga tgtgagatcc tgagggagcg tacgcctgcg ggtctccgct
      901 cccctccgtg aagttgttga agaacaacga caagccggcg gtcatgagcc cggattcgtt
      961 accgccctcg aagaacgcgt tcccgaagac gtacaggctg tctgcagcat tggtcatctg
     1021 cagcagggcg ctctgggtga tcgacagatc gcccccggtg gagaacgcgt ggccgttcag
     1081 cacgagcttg ccggtgaact ggatgaacgt gttcgccgga accgagaagt cgccgctcag
     1141 cgtggtggtc cccgcctgaa ccgcaaactg ggtcccgacc gtcacccccg atcccaccga
     1201 tacattggcg ccattgcagt acaggaatgg gaaagtcacg ccggtgggaa ccgtctgacc
     1261 gttcccggta aaaatcgtcg ccgtcaccgc gtaggtcccg ccggcattga aggtcgtatt
     1321 gacccacagc tccccgaccc tgaggaggct cgccgggaag gtggtcaggc cgccattcaa
     1381 ggccatgccc ccgcccacgt acgtggtgtc cgtgctcgtg accactgcgc tggtatcgag
     1441 tcgggcgttg ccgttgacgc cggtcagctg gtcgaagacc acgcccactt tgttccggaa
     1501 ttccacggac ccgaactggt tcgagtgcgt cgtgtcgaag accgccacac tctgcgtcgc
     1561 ggacccgttg aagacggtca ggtgagaggg gccggcatca aacccactgt gcaccgtcac
     1621 cgcgaagttc ccggcgagca cgaacgtgcc cgcggtcaag tgccccgtct ccactccgcc
     1681 ggcgaaggcg gccgtcccct gcaccaccag cgaatccgcg gcgttggtca tggtgatcac
     1741 gccggtcccc gcggtgccga aggcgccggt cgtcgtcaac gtgtggccgc ccagcgtaag
     1801 gccgccggtc acctgcagcg tcgacgccag cacgttgccg gtcacggcgt aagtcccaat
     1861 caccgtcgtg gccaggatcg ggatcgaccc ggcgagtgtc tttccggttc cgttcagcga
     1921 gagggcactc gagacgctcg ggtccgtgaa cgtgccggcc tgtgtgaacg atccgttcac
     1981 caggagcgac gcggtatcca tcgtgacgat cgcgccggac tggatcgaca gaccggccac
     2041 cgagtaagcg gttgcgcgaa tcaccgccga attggccgtg cccggcggta cgatgacact
     2101 gtcggtggcg gtcggaactc ccgccggatt ccagttcccc gcagtgcccc agtcgctcga
     2161 taccgcgccc gtccatgtct tggggggcgc cgcactcccc gtggcgccga acgtgaccga
     2221 caacgcgccg gcggtcgccg agaaactctg cgccgctgtc gacgggccga gggtgacacc
     2281 ggtggaggcg cgaccgagcg agtcggtcac cacctgggca ctgtccaccg caccgcccga
     2341 gagtgccgca aacgtcacgg tgacgcccgg cactccgagg ttgtcggaac cgttcacctg
     2401 caccaccagc ggcagcggca gcggttggct cgcgacgccg gtctggccgt cgccggagac
     2461 tttcaccatg gcggccggct tcgggacgat ccgcacggtg gtcgagtcgc tcaggccagg
     2521 aggggttggg gatatggcgc gaacggtcac cgtcccccgc gtgatggacg cgaccaggcg
     2581 cccggcagga tcgaccgcga cgctgccgga attcgttccc cagccgacgt agagcgaatc
     2641 gggggtcgcc gtgtcgagca ccgtgaggcg cagctgcaag ggatcgcccg aggtgaggac
     2701 ggtatcacgc gggccgatgg tcatgctcgt ggcgcccttg cccggtccca cgtatcccac
     2761 cggaagcggg accggcgccg gcgtcacgcc tcccgtcacc acgaccgatt gcgatccact
     2821 gaacaggatc tcggtaccgc gctcgtagtc caggaagacg tcgaacgtgt cggtcggctg
     2881 ggtcagggag aagctgatgc tgagttgaag agacgtcccg gaggtgaacg ggtaggtctt
     2941 gtcgacgacg acttccgccg tcgagtggcg caccacctgg gcccggacct ggtcgaccgc
     3001 cagcgcgttc cccggagggg tggcgccagt cacgaagcgc ggcgcgagct cgatgcgcgc
     3061 tggaattctg ggcgcgtcga ggtcacgagt gcaggtcacc acggcgaggc cgagcaggac
     3121 tgccgcagcc gcacgtcttt ggggcgcgaa tgatggcata tgagcggcag ggatagtgca
     3181 gacggagagc gaccccgtca aattaagggt ggcacccgtg gaacccttct tcaggaggcg
     3241 tcgtgcgcat cgccgtactt tcgctcgcgt tcctgtctgc gacagccatg tcgttgtcgt
     3301 cgcagacccg tcccgccccc agtgtggatc ccacggtgac ggcccagggt ccccgccctc
     3361 tgcccggccc ggtctacgaa acgccgggat tcacccgggc cgtggcgaac ggcactcgga
     3421 cccgcaccgg tgctccggga ccgaagtact gggtccagca tcctcgctac cagatcgccg
     3481 cgacgctcga ccccatccaa aatcgggtga ccgggcggga aacggtcgta tacgtgaacc
     3541 atgctccgga tgcgctcgat cggatcgcgg tacatctgcg ccagaacgtg ttcgcgcccg
     3601 gcgtcccgcg gcgccagccg gtacccatca ccggcggggt gaccctcagc cgggtcgccc
     3661 tcgacggccg cgctctggtc ctcgaacccc ggcccgcggc ggtgccgatc acggcgcccc
     3721 cggagcagcc tcctcagccc gggcagtacg tcgtcgacgg gaccgtgatg tggatccccc
     3781 tgggcacgcc gctcaagtcg ggagactcac tccggctcga ggtgaactgg agttactcgc
     3841 ccgctccggc gccggccgat ggacgcgagg ggcatgacgg acacgtctac tttcagggct
     3901 actggtatcc ggaaatcgcc gtgtacgacg acgtcgacgg ctgggtggcg gatccgtacc
     3961 tcctggaagc cgagctgtac atggaccacg ccgactacga cgtacggctg acggtaccgc
     4021 ggggctgggt ggtc
//
LOCUS       DASUBP010000276        14477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_622911, whole genome shotgun sequence.
ACCESSION   DASUBP010000276 DASUBP010000000
VERSION     DASUBP010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 14477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14477
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_622911"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="VFU75_10330"
     CDS             <1..597
                     /locus_tag="VFU75_10330"
                     /inference="COORDINATES: protein motif:HMM:TIGR00044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEU5467278.1"
                     /translation="VLAVTKGFGPDAVKAALDAGLTGVGENRVQEALEKMEHPECRGA
                     EWHLIGHLQRNKAKFVPGKFVSVQSVDSVELAEELDRRASAQKVRLSVLVQVNVAGES
                     QKSGCEPAEVPGLVRRLTQLESLTVDGLMAMAPLTDDEAIQRRAFRGLREIRDRLKED
                     GTWLQTLSMGMSGDFGTAVQEGATLIRLGTALFGARTT"
     gene            594..1082
                     /locus_tag="VFU75_10335"
     CDS             594..1082
                     /locus_tag="VFU75_10335"
                     /inference="COORDINATES: protein motif:HMM:NF016959.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEU5467279.1"
                     /translation="MTGSPEAPEAAFALTPLDIRRQEFRRTLRGYDPAGVEEFRMRVA
                     DELEKVIRERSVLEERVSALTEQLRVFRERENAMNEALVAAQQLRAETRETAAREAQV
                     INREAQAEARTITDDARKAEADIRERVAEVQRQFQGYLAGFRALLERQAAELRALERQ
                     DG"
     gene            1111..4269
                     /gene="ileS"
                     /locus_tag="VFU75_10340"
     CDS             1111..4269
                     /gene="ileS"
                     /locus_tag="VFU75_10340"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEU5467280.1"
                     /translation="MPYPQLPTDLTADALEKQLLATWKHERLFPRVQEIARYGPPFVF
                     FEGPPTANGRPGIHHVFARTIKDLICRFQSMQGKSVTRIAGWDTHGLPVEIEVEKQLQ
                     ISGKKQIEAYGVEAFNKLCRDSVFTYKTDWESLSDRVGYWLDYEHPYVTYSNDYIETV
                     WWLLRRLYDKALLYRGYKVLPYCPRCGTALSSHELAQGYESVQTNSVFVSFPLEKEPN
                     RQLVIWTTTPWTLLANVAVAVHPDLEYGEYELDGVRYIASSALAPSIHVGGKPLTSGT
                     RVKGFLGRDLIGLRYTRPLEVVPLPQEGQRGVVVAGGFVSAEEGSGLVHMAPAFGADD
                     YAVSQQYGLAFVNPVALDGTFTGTRWPEIEGKLVTDKETNRLVIERLKKDGRWLDTKP
                     ITHTYPFCWRCDSALIYYAKSSWFIRTTAFKARMIELNAQVDWHPAEVGSGRFGGWLE
                     NNVDWALSRDRYWGTPLNVWECDRTPEHRDVIGGYGDLAARWGRDLPEDFDPHKPYID
                     GYTWTCATCGGTMRRVPEVIDAWFDSGSMPYAQWHYPFEHAADFQAHFPADYICEGVD
                     QTRGWFYSLLAIAAGTFDAPAFKHVIVNELVLDAQGQKMSKSRGNVVNPWSAIEQYGA
                     DAIRLYLLGQSQVWLPKRFDAKQIPELAGGFLNTLRSTYDFFARYAEDWTPPAEGKEV
                     PFEERPLVDRWLLARLDEVVAAVRQAWGGYDVTAGVRAIMDFVTEDLSRWYVRRNRPR
                     FWAPDRATDPLALEAIHEALTGAARLLAPAAPFLSDWLHRALTGTSVHLAPFPGDRGR
                     RDQGLMDAMAAVRRLASLAHAGRQEKGLNVRQPLARLQVAVPASVKGPALADLLDILA
                     SEVNVKQVEVVESDHDLVSLKGKGNFRSLGPRYGKDTPKVVEAIAALGSEQLQALERR
                     ETVRVGEWDVTPEDVTITREVTSDWVVQSAGPYVVALDPHLTSDLEQEGSAREVVNRV
                     QRLRKDAGYEYTTRIELGISGDAGLVAACEAYRDFIAGETLAKKLVFGPLTEADQRRD
                     VDIDGRTVTLAVRRHEGRKGTR"
     gene            4271..4693
                     /locus_tag="VFU75_10345"
     CDS             4271..4693
                     /locus_tag="VFU75_10345"
                     /inference="COORDINATES: protein motif:HMM:NF013427.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HEU5467281.1"
                     /translation="MPTKASTKSSKNGLNKKQLAHLEKRLLEERARVMKELGHYDESF
                     NSSLQSANGDLSSYSFHMADQGTDAMEREKAFLFASQEGRYLWHVNEALRRLYRTPEK
                     FGRCEQCGEEIGFDRLDALPHARLCIKCKAKEEDGKRR"
     gene            4677..5222
                     /gene="lspA"
                     /locus_tag="VFU75_10350"
     CDS             4677..5222
                     /gene="lspA"
                     /locus_tag="VFU75_10350"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEU5467282.1"
                     /translation="MASDADRRLFWFTAVGVVAADLTTKLIAASALATRPRPVVGDFA
                     VLRLVYNEGAAFGISLGPASRWIFLVLALIALVVLGAMVRATKPGDKLRLLALALVCG
                     GAAGNLLDRIRSAQGVVDFIEVGIGSHRFPTFNVADSAITIGAIALAVSLWLEGRHVT
                     HTAPVPQPPPDTAKEAGAATP"
     gene            5219..6235
                     /locus_tag="VFU75_10355"
     CDS             5219..6235
                     /locus_tag="VFU75_10355"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEU5467283.1"
                     /translation="MTGVTVSFSIVVAVTERLDRFLADQLSLSRTQAARLVADKRVAA
                     AGKTLRASALLERGTEVTVAFPPEAQVRTYAPAHADLSFVYEDDDLAVLDKPAGLVVH
                     PGPGHWDDTLVNVLAGRGMDLSEGSGEGRPGIVHRLDRDTSGLLLLAKTDLAHRRLAG
                     AIERREVRRVYATVTWGHLAKSPLDIEAPIGRNPKDRKRMTVAAGGRHAVTHARVIAR
                     FELTELLRVRLGTGRTHQIRVHLGHVGHPVVGDRDYSFGGARRVTPSLRRAAEALDDL
                     APRQLLHAALLSFKHPVSGAPVEVRSEWPEDLRAALAETSGDANLLAQRNPLQYLGFF
                     DSDD"
     gene            6228..6671
                     /locus_tag="VFU75_10360"
     CDS             6228..6671
                     /locus_tag="VFU75_10360"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEU5467284.1"
                     /translation="MIDSAVVRLLVFRVGTLSCAAEVETVREILPRLTVTRIPGAPPE
                     VAGMVNVRGALVTVVDGWRALGQAAPEPGSGTTVLLEVEGGRRLMGLAVDEVLDLLAV
                     SGEALEQRQSLPGLDPTIVRAVGRRGTQAFVVLDTDRLLGPILSS"
     gene            6681..7043
                     /locus_tag="VFU75_10365"
     CDS             6681..7043
                     /locus_tag="VFU75_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5467285.1"
                     /translation="MSHTVLVCDDAIFMRTMISDILAQAGFEVVGEAETGAQAVEKYK
                     QLKPDLVTMDIVMPDMGGIDAVREIVKGDPDAKILMCSAMGQQALVVEAIQAGAKDFV
                     VKPFQPSRVLEAVQRVLG"
     gene            7047..8978
                     /locus_tag="VFU75_10370"
     CDS             7047..8978
                     /locus_tag="VFU75_10370"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HEU5467286.1"
                     /translation="MDLSQYAELFAAEAREHLSACNQLLLQWERAPGAAEPVTGLFRA
                     VHTIKGMAATMGYSVVADLAHRMENLLDALRRGAVPPGDEVLQLLFRSTDALERAVGL
                     AVAGKDREADTRGLGGELDRAAAKAGAPAAGSAARPSGPEEPPAAGRPSVPVLAIPEL
                     ASPAAGRLVQVMLRSDAQLKAGRALLVLKRVEALGRVSGVTPPQAAFESDDFDGRFAF
                     RLDGDAAPDVIEKAARTAGDVERVVVGEREAEAPQLDAAAGPGDGGRARHIRVDLRRL
                     DVLMDLIGELTTARGRLNQLAGDRRDGPLDDVALQISRLSSDLQNEIVQARMTPVWQV
                     FDRFPRLVRDIARQLERHVSFRVEGKEIELDRAILDELGDPLLHLLRNAVDHGIEPSA
                     ERRKAGKKEEGEVVLAAVRERASVAITISDDGRGIDRRKILQKAKAEGSVDAHVDALT
                     DDQLLRVIARPGFSTAAAVTNLSGRGVGIDVVMSRIRALGGTVEIHTEVGKGTTFVLR
                     MPVTLAIVRALIAAVGNERYALPLTYVSETVEFGAVPTTTVEGREAMVLRDRVVPLVH
                     LRGLLGVEGDAPPARRPVIVLEMGERRTGVVVDGMLGQQEIVVKGFEAPRGTLPVFSG
                     ATIMGDGVPALILDAGGLL"
     gene            8992..9609
                     /locus_tag="VFU75_10375"
     CDS             8992..9609
                     /locus_tag="VFU75_10375"
                     /inference="COORDINATES: protein motif:HMM:NF016398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheC"
                     /protein_id="HEU5467287.1"
                     /translation="MDDVRDLKTLQIDALREVANIGAGHAATALSQLTNRRIMISVPQ
                     INITRLEDVPNLLGNPQDVVAAVLMHMLGDLTGRTLLLFPEQTARRLCDMLLRRPEKT
                     TTAFDQLEQSALKEAGNILSGAYMNALSDFMGMLLLPSVPSLVVDLSAAVLTTTYLNF
                     GHDRDFVFCVETEFRFDAEDGLRGHFLLLPDLASLKAIFDAIRVT"
     gene            9613..12312
                     /locus_tag="VFU75_10380"
     CDS             9613..12312
                     /locus_tag="VFU75_10380"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5467288.1"
                     /translation="MAAAALSDKDLAVLRSFARRIDPSDAGAHNNLGVLYYQKGLVSE
                     AIAEFTRALELDAKMQVAQRNLEIAYHNSGYYDQLVAQLRERLRAAPDERDARWELGR
                     AYHILGVYDAAVGEFEQLLAHHPKDTAAMLQLGQAEKSRGRFEAATEWLLKAQELEPT
                     SSVVQFYLGEVYYNRGLNDAALTALQRAVELNPDNANAHYLMAFVLGDMGRHQDARTA
                     SKRAIELNPPLARAQANLSLERYNAERKSQQLRLRDLPETEIVEGAELAHYNLGLAFR
                     QKGYYAEALREYRLALDRGEDRRMTLQAMAEVHLLRRDLAAALELYDTLLQEIPQSPK
                     LWNERGVVLHQAGRIDDALVAYRQAIEVDGRYALAWNNTGVVLGHKGATDEAIESFRQ
                     ALRAQSDFPVARLNLALLLFHLRRFQLSLEAYRAVLGEQPKSAPAWNGVGLVLLELKR
                     YPDARNAFARAVEADADHAGAHYNLSFALSNLGDFDGALRATKRALELDPYYVSQKFQ
                     LTIDLQFEKTTIGVAPEISADVSTETLGTDFSFDQRILDNIFQDLAPPVPATAPAVPG
                     KPKDDALALARDYVSKGLHDLATGEAIRAVSRGADQAEASVLIGGSYAKRGLHGEALE
                     RFREARALDAERVDARAGEVQALVAMGRMQDAAPLAEAWLTRAPGDVDALVAVAKARA
                     GAGDAAGALTALQEAQQRAPGRADLHKLQGEVLIKVGDRAGALAAFRTALELDRGYVQ
                     VWLDLGLLHEEKEEWSEAQRAYENALEVLPTFHEANLALAELLRKSGRVRLAIAKLAD
                     LLEQDPYDLDALILLGRALLDDKRLDASAEAFRRVLKFDPDHVGALFHLGVTLARRHR
                     YPEAVRAWDKVIKLDAGGTYASRAKMHARTAADLQTIFASDAA"
     gene            12318..13469
                     /locus_tag="VFU75_10385"
     CDS             12318..13469
                     /locus_tag="VFU75_10385"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEU5467289.1"
                     /translation="MPIEGPLKELGIHDVFQLLDLSRKTGALRVTSELRHNSGTVYFE
                     NGAIIFAQIQSNPHPLGALLIRTGKITEADLERSRDRQRKGDTRRVGEILVEMGAITN
                     RELQRQVRFQVEEVVFEVMSWQEGYFSFSEGQDGKFPAEASVRIPTEALLMEGARRID
                     EWSRIEKRVPHVGVVPSFASATEGQPGDLDLLPPEWEVLALIDGERDIRGVASELGRS
                     EFDVAKTLFGLESAGVITFGEKNLAPGARRTSAVTELVAQAEGALKDRDLEKAKAAAE
                     QAVALHAHDPGVHLLLGQVHLAAGRASDAVEELRRTVRLDPLLAPAHRMLGFALVGVG
                     RFADGVEEWDRWERLAAQSEPEMAFLEEVRRARSAAQILGGLGAGGGRG"
     gene            13462..14283
                     /locus_tag="VFU75_10390"
     CDS             13462..14283
                     /locus_tag="VFU75_10390"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5467290.1"
                     /translation="MADDIKLLSAQLAQDPHSLVFLRLGELLRQKRQFDLAQRVATNG
                     LERHPHLADAHDLLARIMADRHDYEHAFDEWDMTLRIAPGHTGALKGLAFLYFKVGDL
                     AQAESHLREAKKVDPDDPAVDQALAMVRGGAAAPGGTAPAVPVSTEVAEPIPEERVFA
                     GLEGAQEGLLLLDAAGQVLGGALKSADGTDTTQVVAAYLAGVSQEASRTAKLLGLGGW
                     IGLSAEGPQGNVHLTPPTNDALLLVVRDRSVPMGRLAVIAQRASLLARRWLERQV"
     gene            14280..>14477
                     /locus_tag="VFU75_10395"
     CDS             14280..>14477
                     /locus_tag="VFU75_10395"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEU5467291.1"
                     /translation="MSANGYEAALRGITSVRGVRGAMVVAGADGLVVAESLMEGIKGA
                     AVAALAASLATRLGRAMEAGGV"
BASE COUNT         2333 a         4745 c         5215 g         2184 t
ORIGIN      
        1 gtgctcgccg tcacgaaggg gttcgggccc gacgccgtaa aggcggcgct tgatgcggga
       61 ttgaccggcg taggtgagaa tcgggtacag gaagcgctgg agaagatgga gcacccggag
      121 tgccgcggtg cggagtggca cttgatcggc cacctccagc gcaacaaggc gaagttcgtg
      181 ccggggaagt tcgtctcggt gcagtcggtg gactcggtgg agctggccga ggagctggat
      241 cgccgggcct cggcacaaaa ggtgaggctg agcgtactgg tgcaggtgaa cgtggcgggt
      301 gagtcgcaga agagtggatg cgagccggcg gaggtcccgg ggctggtccg gcggctgacg
      361 cagctcgaga gcctgacggt ggacgggctg atggcgatgg caccgctcac cgacgacgaa
      421 gcgatccaac gtcgggcctt ccggggactc cgggagatcc gcgatcgact gaaggaggac
      481 ggcacatggc tgcagaccct ctcaatgggg atgtccggcg acttcggtac ggcagtgcaa
      541 gagggagcaa cgctgatccg cttgggtacc gccctcttcg gagcgaggac gacgtgaccg
      601 gttcgcccga agctcccgaa gccgccttcg cgctcacgcc gctcgacatc cggcgccagg
      661 agttccggcg cacgctgcgc ggatacgatc ccgcaggcgt ggaggaattc cgcatgcggg
      721 tggccgacga gctcgagaaa gtgattcggg agcgctcggt gctcgaagag cgggtgtccg
      781 cgctcaccga acagctccgc gtgttccggg aacgcgagaa cgcgatgaac gaggccctcg
      841 tcgccgccca gcagctccgg gccgagaccc gggagacggc ggcgcgcgag gcgcaggtga
      901 tcaaccgcga ggcgcaggcg gaggcgcgga cgatcacgga cgacgcccgc aaggccgagg
      961 cggacatccg cgagcgggtg gccgaggtgc agcggcaatt ccagggatac ctggcggggt
     1021 tccgggcgct cctcgagcgt caggccgccg agctgcgggc gctggaacgg caggacgggt
     1081 gagggaaccg ctgaacccgc ttcgcacctg atgccgtacc cgcagctgcc caccgacctc
     1141 accgcggacg cgctggagaa acagctcctc gcgacctgga agcacgagcg cctctttccc
     1201 cgggtccagg aaattgcgcg ctacgggccg ccttttgtgt tcttcgaagg gccgcccacg
     1261 gccaacggcc gccccgggat tcaccacgtc ttcgcgcgca ccatcaagga tctgatctgc
     1321 cgcttccagt cgatgcaggg gaagagcgtc acccgcatcg ccgggtggga cacgcacggc
     1381 ctgccggtgg agatcgaagt cgaaaagcag ctgcagatct cgggcaaaaa gcagatcgaa
     1441 gcgtacggcg tggaggcctt caacaagctc tgccgggatt cggtcttcac ctacaagacc
     1501 gactgggagt cgctctccga ccgggtggga tactggctcg actacgagca tccctacgtc
     1561 acgtactcca acgactacat cgagaccgtc tggtggctgc tccggcgtct ctatgacaag
     1621 gcgctgctct atcgcgggta caaggtgctc ccgtattgcc cgcgctgcgg cacggcgctg
     1681 tcgtcgcacg agctggcaca ggggtacgag agcgtccaga ccaactcggt cttcgtctcg
     1741 ttccccctcg agaaggaacc gaatcgccag ctggtgatct ggaccacgac cccgtggacg
     1801 ctgctcgcca acgtcgcggt cgccgtgcac cccgatctcg aatacggcga gtacgaattg
     1861 gacggggtcc ggtacatcgc gtcctccgcg ctcgcgccgt cgatccacgt gggcggcaag
     1921 ccgctcacgt cgggcacccg ggtgaaggga ttcctggggc gcgatctcat cggactccgg
     1981 tatacgcgcc cgctcgaggt ggtgcccctg ccgcaggaag gacagcgtgg cgtcgtggtg
     2041 gccggcgggt tcgtgtcggc ggaggaggga tcggggctgg tccacatggc gccggccttc
     2101 ggtgccgacg actacgcggt ctcgcaacag tacggtctcg ccttcgtgaa tccggtcgcc
     2161 ctcgacggga cgttcaccgg gacgcggtgg cccgagatcg agggcaagct cgtcaccgac
     2221 aaggaaacca accggctggt gatcgagcgg ctgaagaagg acggccggtg gctggacacc
     2281 aagcccatca cccacaccta tcccttctgc tggcggtgcg actcggccct catctactac
     2341 gccaagagct cgtggttcat ccggaccacg gcgttcaagg cgcggatgat cgagctcaac
     2401 gcgcaggtgg actggcatcc ggccgaggtg gggagcggcc gcttcggcgg ctggctggag
     2461 aacaacgtcg actgggcgct ctcccgcgac cggtattggg gcacgccgct caacgtctgg
     2521 gagtgcgacc gtacccccga gcaccgggac gtcatcggcg ggtacgggga tctcgcggcg
     2581 cggtggggga gggacctgcc cgaagacttc gacccgcaca agccgtacat cgacgggtac
     2641 acgtggacct gcgcgacctg cggcgggacc atgcgccggg tgcccgaggt gatcgacgcc
     2701 tggttcgact cgggctccat gccgtacgcg cagtggcact atcctttcga gcacgccgcg
     2761 gatttccagg cgcacttccc ggcggattac atctgcgaag gcgtggacca gacgcgcggg
     2821 tggttctact cgctcctcgc gatcgcggcg ggcacgttcg acgcgccggc gttcaagcac
     2881 gtcatcgtga acgagctggt gctggatgcg cagggccaga agatgtcgaa gagccgcggg
     2941 aacgtcgtga acccatggtc cgccatcgag cagtacggcg ccgacgccat ccgcctctat
     3001 ctgctcggac agagccaggt ctggttgccg aagcggttcg acgcgaagca gatccccgag
     3061 ctcgcgggcg gcttcctcaa tacgctgcgc tcgacgtacg acttcttcgc gcgctacgcc
     3121 gaggactgga cgccgccggc ggaaggcaag gaagtgccgt tcgaggagcg gcctctggtg
     3181 gatcgctggc tgttggcgcg actcgacgag gttgtggcgg cggtccggca ggcgtggggc
     3241 gggtacgacg tgacggcggg tgtgcgcgcc atcatggatt tcgtcacaga agatctgtcc
     3301 cgctggtacg tccgccggaa tcggccccgc ttctgggcgc ccgaccgcgc caccgacccg
     3361 ctggccctcg aggcgattca cgaagccctg accggcgcgg cgcggctgct cgcgcccgcg
     3421 gcacccttct tgtcggattg gctccaccgg gccctgactg gaacctccgt ccacctggcg
     3481 ccgttcccgg gggatcgcgg gcgccgggat caggggctga tggacgcgat ggcggcggta
     3541 cggcggctgg cgtccctggc ccacgccgga cggcaggaga agggcctgaa cgtccgccag
     3601 cccctggcac gcctccaggt ggcggtaccg gcctcggtga agggcccggc cttggcagac
     3661 ctgttagata tcttggcctc tgaagtcaac gtgaaacagg tggaagtggt cgaatcggat
     3721 cacgatctcg tgagcttgaa ggggaagggc aacttccgga gccttgggcc tcggtacggc
     3781 aaggacacgc ccaaagtggt cgaggcaatc gcggcgttgg ggagcgaaca gctgcaggcg
     3841 ctcgagcgta gggaaacggt gcgcgtgggg gagtgggacg tcacccccga agacgtcacg
     3901 atcacccggg aagtcacgag cgactgggtc gtgcaatccg ccggaccgta cgtggtggcg
     3961 ctcgatccgc atctcacgtc cgacctggaa caggaagggt cggcgcggga ggtggtgaac
     4021 cgggtgcagc gactccgcaa ggatgcgggg tatgagtaca cgacgaggat cgagctggga
     4081 atttcgggcg atgccggttt ggtggccgcc tgtgaagcgt atcgggattt catcgccgga
     4141 gaaacgctgg cgaagaagct ggtgttcggt ccgctgacgg aagcggacca gcgcagagac
     4201 gtcgacatcg acggacgcac ggtgaccctc gccgtgcgac gccacgaggg aagaaagggc
     4261 acgcgataac atgccgacga aggccagcac gaagagcagc aagaacggat tgaacaagaa
     4321 gcagctcgcg catctcgaga agcggttgct cgaggagcgc gcccgggtca tgaaggagct
     4381 cgggcactac gacgagtcct tcaactcgag cctgcagagc gccaacggcg atctgtcgag
     4441 ctactccttc cacatggccg atcagggcac cgatgcgatg gagcgagaga aggccttcct
     4501 cttcgcgtcg caggaggggc ggtatctctg gcacgtgaac gaggccctgc ggcggctcta
     4561 ccgcacgccc gagaagttcg gccggtgcga gcagtgcggt gaagagatcg gcttcgaccg
     4621 gctcgacgcg ctgcctcacg cgcgcctgtg catcaagtgc aaggcgaagg aagaagatgg
     4681 caagcgacgc tgatcgccgg ctcttctggt tcaccgcggt tggggtggtc gccgccgacc
     4741 tgaccaccaa gctgatcgcg gccagcgcgc tggcgacgcg tccgcgtccg gtggtggggg
     4801 acttcgccgt cctgcggctg gtctacaacg aaggcgccgc gttcggaatc tccctcggtc
     4861 ccgcctcccg ctggatcttc ctcgtcctgg cgctgattgc gctggtggtc ctgggcgcga
     4921 tggtgcgggc gaccaagccc ggggacaagc tccgtctgct ggcactcgcc ctggtctgtg
     4981 gcggcgcggc gggcaacctg ctcgaccgga tccggtcggc gcagggagtc gtggacttca
     5041 tcgaggtcgg catcggctcc caccgtttcc cgaccttcaa cgtcgcggac tcggccatca
     5101 ccatcggagc gatcgcgctg gcggtctcgc tctggctgga aggccggcac gtgacgcaca
     5161 ccgctccggt gccgcaacct ccccctgaca ccgcgaagga ggctggagcc gccacgccgt
     5221 gaccggcgtg acggtatcct tctccatcgt cgtcgcggtg acggagcggc tggaccgctt
     5281 cctcgccgat cagctctccc tctcgcgaac ccaggcggcc cggctcgtcg ccgacaagcg
     5341 cgtggccgcc gctggaaaaa ccctgcgcgc gtcggcgctc ctcgaacgcg gcaccgaagt
     5401 caccgtcgca tttccccccg aagcgcaggt ccgcacctac gcccccgcgc acgccgatct
     5461 ctcgttcgtc tacgaagacg acgatctcgc ggtactggac aagcccgcgg ggctggtcgt
     5521 gcaccccggg cccggccatt gggacgacac gcttgtgaac gtgctcgcag gccgcgggat
     5581 ggatctgtcc gaggggagcg gcgagggccg tccggggatc gtgcaccggc tggatcgcga
     5641 cacgtcgggg ctgttgctgc tcgccaagac cgatctcgcg caccggcggc tcgccggtgc
     5701 catcgaacgc cgcgaggtcc ggcgggtcta tgcgacggtc acctggggac acctggcgaa
     5761 gagcccgctc gacatcgaag cgcccatcgg ccggaatccc aaggaccgaa agcggatgac
     5821 ggtggcggca gggggccggc acgccgtgac ccacgcgagg gtgatcgcca ggttcgagtt
     5881 gacggagctc ctgcgcgtgc ggctgggcac ggggcgcacc caccagatcc gggtccacct
     5941 gggtcacgtc gggcatccgg tggtggggga tcgcgactac tcgttcgggg gcgcccgtcg
     6001 cgtgacgccc tccctccggc gggccgccga ggcgctcgac gatctggcgc cgcggcagtt
     6061 gctccatgcc gcgctccttt cgttcaagca cccggtgtcg ggcgccccgg tggaagtccg
     6121 gtcggagtgg cccgaggatt tgcgcgccgc actcgccgaa acgagtggcg acgcgaattt
     6181 gcttgctcaa cgcaacccct tgcagtatct tggtttcttc gattctgatg attgattcag
     6241 cggtcgttcg gctgctcgtt ttccgggtag ggacgctctc ctgcgcggcc gaagtcgaga
     6301 ccgtacggga gattctgccg cgtctcaccg tgacgcggat ccccggtgca ccccccgaag
     6361 tcgcgggaat ggtgaacgtc cggggcgcct tggtgacggt ggtggacggg tggcgggcgc
     6421 tgggccaggc cgcccccgaa ccggggagcg gcaccaccgt gctcctcgaa gtcgaaggcg
     6481 ggcgccggct gatggggctc gcagtcgacg aagtgctgga cctcttggcc gtgtcggggg
     6541 aggcgctgga gcagcggcag tcccttccgg gtctcgaccc gaccatcgtc cgcgcggtcg
     6601 gccgccgcgg cacccaggcg ttcgtcgtcc tcgataccga ccggctgctc ggtcccattc
     6661 tgtcttccta gggaggtcgt gtgagtcaca cggttctcgt ctgcgacgac gccatcttca
     6721 tgcggacgat gatcagcgac attctcgcgc aggcggggtt cgaggtcgtg ggtgaggccg
     6781 agaccggcgc tcaggcggtg gagaaataca agcagctgaa gcccgacctg gtcaccatgg
     6841 acatcgtgat gccggacatg ggcggcatcg acgcggtccg ggagatcgtg aagggcgatc
     6901 ccgacgcgaa gatcctgatg tgcagcgcga tggggcagca ggccctggtg gtggaggcga
     6961 ttcaggccgg cgccaaggat ttcgtcgtca aacctttcca gccctcccgg gtgctcgagg
     7021 ccgtccagcg ggtcctcggg taagcaatgg atctgtcgca gtacgccgag ctgttcgcgg
     7081 ctgaagcccg cgagcacttg agcgcatgca accaattgct gttgcagtgg gagcgcgcgc
     7141 cgggcgctgc ggaacccgtc accggcctct tccgtgccgt ccacaccatc aaggggatgg
     7201 ccgccacgat ggggtacagc gtggtggcgg acttggcgca tcggatggag aacctcctcg
     7261 acgcgttgcg ccggggagcg gtccctcccg gcgacgaggt gctccagctc ctgttccggt
     7321 cgaccgacgc actggagcgc gcggtgggcc tcgcggtcgc cggcaaggac cgcgaagccg
     7381 atacgcgcgg actcggcggg gagctcgacc gggcggcggc gaaggccggc gcgcccgcgg
     7441 ccgggtcggc ggctcggccc tcgggcccgg aagaaccgcc ggccgcggga cgtccgtcag
     7501 tgccggtgct ggcgattccc gagctggcat cgccggcggc gggacggctg gtgcaggtga
     7561 tgctgcggtc cgacgcgcag ctcaaggcgg ggcgggcgct gctcgtactg aagcgggtcg
     7621 aagcgctggg ccgggtgagc ggggttaccc ctccccaggc cgcgttcgag tccgacgatt
     7681 tcgacggacg ttttgcgttt cgcctggacg gggacgcggc gccggacgtg atcgagaagg
     7741 cggcgcgcac ggcgggagac gtcgagcgcg tggtggtggg cgaacgggag gcggaagcgc
     7801 cgcagctgga cgccgccgcc ggtccgggtg atggaggccg cgcccggcac atccgggtcg
     7861 acctccggcg cctcgacgtg ctgatggacc tcatcggcga gcttacgacg gcgcgcggcc
     7921 ggctcaatca gctggccggg gaccgccgcg acgggccgct cgacgacgtg gcactgcaga
     7981 tctcgcggct ctcctcggat ctgcagaacg agatcgtcca ggcccgcatg acgccggtgt
     8041 ggcaggtgtt cgaccggttt ccgcggctgg tccgggatat cgcgcggcag ctcgagcgcc
     8101 acgtgtcgtt ccgggtggaa gggaaggaga tcgagctcga tcgcgcgatc ctcgacgagc
     8161 tcggcgatcc gctcctccac ctcctgcgga acgccgtgga ccacggcatc gagccgtcgg
     8221 cggagcggcg gaaggcggga aagaaggaag aaggcgaagt ggtgctcgcg gcggtgcggg
     8281 aacgcgccag cgtggcgatc accatctcgg atgacggacg cggcatcgac cggaggaaga
     8341 ttctccagaa ggcgaaggcc gaaggctcgg tcgacgccca cgtcgatgcg ctgaccgacg
     8401 atcagctgct ccgcgtcatt gcccggccgg gattctcgac cgccgcggcc gtgacgaacc
     8461 tgtcggggcg cggcgtcggc atcgacgtgg tgatgagccg gatccgggca ctcgggggca
     8521 cggtcgagat ccacaccgag gtcgggaagg gcaccacctt cgtcctgcgg atgccggtga
     8581 cgctggcgat cgtgcgtgcg ttgatcgccg cggtggggaa cgagcgctac gcgctgcccc
     8641 tcacgtacgt gtccgagacg gtggagttcg gcgccgtccc gacgacgacg gtggagggac
     8701 gcgaagcgat ggtgctgcgg gaccgggtgg tcccgctcgt gcacttacgc ggcctgttgg
     8761 gtgtggaggg cgacgcgccg cccgcgcggc ggccggtgat cgtgctggag atgggggaac
     8821 gacgcaccgg cgtcgtggtg gacggcatgc tgggccagca ggaaatcgtg gtgaagggat
     8881 tcgaggcgcc gcggggcacg ctgccggtgt tcagcggagc gaccatcatg ggtgacgggg
     8941 ttccggcgtt gattctggac gccggcggac tgctgtaggc gggggaaagt catggacgac
     9001 gtccgcgatt tgaagacgct gcagatcgac gcgctccgcg aggtggccaa tatcggggcc
     9061 ggccacgccg cgacggcgct ctcgcagctc accaaccgcc gcatcatgat ctcggtgccg
     9121 cagatcaaca tcacgcggct cgaagacgtc cccaacctgc tgggcaatcc gcaggacgtc
     9181 gtcgccgcgg tcctgatgca catgctcggc gatctgaccg gccgcaccct gctcctgttt
     9241 ccggagcaga cggcacgccg gctctgcgac atgctgctgc gccgtccgga gaagaccacc
     9301 accgccttcg atcagctgga gcagtcggcc ttgaaggaag cggggaacat cctttcgggc
     9361 gcctacatga acgccctctc cgacttcatg gggatgctcc tcctgccgtc ggtgccgtcg
     9421 ctggtggtgg acctctcggc cgccgtcctg accaccacgt atctgaactt cggtcatgac
     9481 cgcgatttcg tcttctgtgt ggaaacggag ttccgcttcg acgccgagga cggcctgcgg
     9541 gggcacttcc tgctcctccc cgacctggcg tcgctcaagg cgatcttcga cgccatccgc
     9601 gtgacctgac ccgtggcggc agcggcgctc tccgacaagg acctcgccgt cctgcggtcg
     9661 tttgcccgcc ggatcgaccc ttcggacgcc ggggcgcata acaacctcgg cgttttgtat
     9721 taccagaagg gcctcgtctc ggaggcgatc gccgagttca cgcgcgccct cgagctcgac
     9781 gccaagatgc aggtggccca gcgcaacctg gaaatcgcct accacaacag cgggtattac
     9841 gaccagctgg tggcccagtt gcgggagcgg ctccgcgccg cgcccgatga gcgggacgcg
     9901 cgctgggagc tggggcgcgc gtatcacatc ctcggtgtgt atgacgccgc cgtgggggaa
     9961 ttcgagcagc tcctcgccca tcatccgaaa gacaccgcgg ccatgctgca gctggggcag
    10021 gccgaaaaga gccgcggccg gttcgaggcc gcgaccgagt ggctcctcaa ggcgcaggag
    10081 ctcgagccca cgtcttcggt ggtccagttc tacctcggcg aggtctacta caaccgcggc
    10141 ctgaacgacg cggcgcttac ggcactgcag cgcgccgtgg agctgaaccc cgacaacgcg
    10201 aacgcccact acctcatggc gttcgtgctg ggcgacatgg ggcggcacca ggacgcgcga
    10261 acggcgtcga agcgcgccat cgagctgaac ccgccgctcg cgcgcgcgca ggccaatctc
    10321 tcgctcgagc ggtacaacgc cgagcggaag agccagcagc tgcgcctccg cgatctcccc
    10381 gagaccgaaa tcgtcgaagg cgccgagctc gcgcactaca acctggggct cgcgttccgg
    10441 cagaagggct actacgccga ggcgctgcgg gagtaccggc tcgcgctcga ccgcggcgaa
    10501 gaccggcgca tgaccctgca ggcgatggcg gaggtgcatc tcctccggcg cgatctcgcg
    10561 gccgcgctcg agctgtacga cacgctgttg caggagatcc cgcaatcgcc gaagctgtgg
    10621 aacgagcggg gcgtggtcct gcaccaggcg ggacgaatcg acgacgcgct cgtcgcgtac
    10681 cggcaggcca tcgaagtgga cgggcgctac gcgctcgcct ggaacaacac cggggtggtg
    10741 ctcggccaca agggcgccac cgacgaggcc atcgaatcgt tccggcaggc gctccgggcg
    10801 cagagcgatt tccccgtggc gcggctcaac ctcgcgctcc tgctctttca cctgcgccgg
    10861 ttccagctgt cgctcgaagc ctaccgcgcc gtcctcggtg aacagcccaa gtcggcgccg
    10921 gcgtggaacg gcgtggggct cgtgctcctc gagctcaagc ggtacccgga cgcccgcaac
    10981 gcgtttgccc gggccgtgga ggccgacgcg gaccacgccg gggcacacta caacctctcg
    11041 ttcgccctct ccaacctcgg ggatttcgac ggagcgctcc gcgccaccaa gcgcgccctg
    11101 gagctggatc cgtactacgt gtcgcagaaa ttccagctca ccatcgacct gcagttcgag
    11161 aagacgacca tcggggtcgc gccggagatc tccgcggacg tctcgaccga gacgctcggg
    11221 accgacttct ccttcgacca gcgcatcctc gacaacatct tccaggatct ggcgccgccg
    11281 gtgccggcca ccgctcccgc cgtgccgggc aagccgaagg acgacgcgtt ggccctggcc
    11341 cgcgactatg taagcaaggg cctgcacgat ctcgcgacgg gcgaagcgat tcgcgccgtg
    11401 tcacgcggtg ccgaccaggc cgaggcctcc gtgctgatcg gcgggagcta cgcgaagcgc
    11461 gggctgcacg gggaagccct ggagcggttc cgcgaagcgc gggccctgga tgcggaacgg
    11521 gtggatgccc gggcggggga ggtgcaggcg ctcgtggcga tggggcgcat gcaggacgcc
    11581 gcgcccctgg ccgaagcgtg gctcacccgc gcgcccggtg acgtggacgc gctcgtggcc
    11641 gtcgccaagg cacgcgccgg ggccggcgat gcggccggcg cgctgaccgc cctgcaggag
    11701 gcgcagcagc gcgccccggg ccgtgccgat ctccacaagc tccagggcga agtgctgatc
    11761 aaggtggggg atcgggccgg agccctggcc gcgttccgga ccgcgctcga gctggaccgc
    11821 gggtacgtcc aggtctggct cgatctggga cttctgcatg aagagaagga agaatggagt
    11881 gaggcccagc gcgcctacga gaacgcgctc gaggtgctgc cgaccttcca cgaagccaac
    11941 ctggccctgg ccgagctgct gagaaagagc gggcgggtgc ggttggcgat cgcgaagctg
    12001 gcggatctgc tcgaacagga tccgtacgat ctcgacgcgc tcatcctgct cgggcgcgcg
    12061 ctcctcgacg acaagcggct cgatgcgtcc gcggaagcgt tccggcgggt tctcaagttc
    12121 gaccccgatc acgtcggcgc gctgttccac ctgggcgtga cgctcgcgcg ccggcaccgg
    12181 tatccggagg ccgtccgcgc gtgggacaag gtgatcaagc tcgatgccgg cggcacctac
    12241 gcgtcccggg ccaagatgca cgcccgcacc gccgccgacc tccagaccat cttcgcatcg
    12301 gacgcggcct agaacccatg cccatcgaag gcccgctgaa ggaactgggg atccacgacg
    12361 tcttccagct gctcgacctg agccggaaga cgggggcgct gcgcgtcacc tccgagctgc
    12421 gccacaattc cggaaccgtg tacttcgaga acggcgcgat catcttcgcc caaatccaga
    12481 gcaacccgca cccgctcggt gccctcctga tccggaccgg caagatcacg gaggccgacc
    12541 tggagcggtc acgcgaccgc cagcggaaag gcgacacgcg gcgggtcggc gagatcctgg
    12601 tcgaaatggg cgccatcacg aaccgcgagc tgcaacggca ggtgcgcttc caggtcgagg
    12661 aagtggtgtt cgaggtcatg agctggcagg agggctactt ctccttcagc gaagggcagg
    12721 atggcaagtt cccggccgag gcgtccgtgc gcattcccac ggaagcgctg ctgatggagg
    12781 gcgcccgccg gatcgacgag tggtcgcgca tcgaaaagcg ggtgccgcac gtcggagtcg
    12841 tcccgagctt tgcctcggcc accgagggcc agcccggcga cctcgatctc ctgcctcccg
    12901 aatgggaagt cctcgccctg atcgacggcg agcgggacat ccgcggagtc gcctccgagc
    12961 tcgggcgctc cgagttcgat gtcgccaaga cgctgttcgg tctcgaaagc gcgggcgtca
    13021 tcacgttcgg ggagaagaac ctcgcgcccg gcgcccggcg tacctcggcg gtcaccgagc
    13081 tcgtggcgca ggccgaaggc gcgttgaagg accgggatct cgagaaggcg aaggcggcgg
    13141 cggagcaagc cgtcgcgctg catgcccacg acccgggcgt gcacctgctg ctcgggcagg
    13201 tgcacttggc cgcgggccgc gcgagcgacg cggtcgaaga gctgcgccgt accgtccggc
    13261 tcgatccgct gctcgcgccg gcgcatcgca tgctcgggtt tgccctcgtc ggcgtcggac
    13321 gcttcgccga cggcgtcgaa gagtgggacc gctgggagcg gctcgcggcg cagtcggagc
    13381 cggagatggc cttcctcgag gaggtccgcc gggcgcgctc ggcggcgcag atcctgggtg
    13441 gcctcggcgc gggaggcggc cgtggctgac gacatcaagc tgctctccgc ccagctcgcg
    13501 caggacccgc acagcctggt gttcctccgg ctcggcgagc tcctgcggca gaagcgccag
    13561 ttcgacctcg cgcagcgcgt ggcgacgaac ggactcgagc gtcacccgca tctggcggac
    13621 gcgcacgacc tgctcgcgcg catcatggcg gaccggcacg actacgagca tgcgttcgac
    13681 gagtgggaca tgaccctgcg catcgcaccg ggtcacacgg gggcgctgaa ggggctcgcg
    13741 ttcctctact tcaaggtcgg cgacctggcg caggcggagt cccacttgcg cgaagcgaag
    13801 aaggttgacc ccgacgatcc cgcggtcgat caagccctgg ccatggtgcg gggcggcgcc
    13861 gcggcgccgg gaggaacggc tccggcggtc cccgtctcga ccgaagtggc ggagccgatt
    13921 ccggaagagc gcgtcttcgc cggcctcgag ggtgcgcagg agggcttgct gctcctggat
    13981 gccgcgggcc aggtcctcgg gggcgcgctc aagagcgccg atggcacgga cacgacccaa
    14041 gtcgtcgcgg cctatctcgc cggcgtgtcg caggaggcga gccgcacggc caagctgctg
    14101 ggactcggag gctggatcgg actgtcggcg gaggggccac agggcaacgt gcacctcacc
    14161 ccgccgacca acgacgcact gctgctggtc gtgcgtgacc gcagtgtccc catgggaaga
    14221 ttggccgtga tcgcgcagcg ggcgtcactg ctcgcgcgcc ggtggctgga gcggcaggta
    14281 tgagcgccaa cgggtacgag gccgcgctgc gcggcattac cagtgtccgc ggggttcggg
    14341 gtgcgatggt ggtcgccggg gccgacggcc tggtggtggc ggagagcctg atggagggca
    14401 tcaagggcgc cgcggtggcc gcgctggcgg cgagtctggc cacccggctg ggacgcgcca
    14461 tggaagcggg aggtgtg
//
LOCUS       DASUBP010000277        14842 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_690963, whole genome shotgun sequence.
ACCESSION   DASUBP010000277 DASUBP010000000
VERSION     DASUBP010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 14842)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14842)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14842
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_690963"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..890)
                     /locus_tag="VFU75_10400"
     CDS             complement(<1..890)
                     /locus_tag="VFU75_10400"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEU5467292.1"
                     /translation="MASIDSNHTRAAILILLLGVGLAIALTPFATGLIGIPVLYVILQ
                     PVHDRLARHVRPTPAAVIVVLLALFLVVVPGVSFVSLVVGQAQQVVAGGASNPLLAAL
                     AHLKIGDTDLGPQLASMTQDVAGWIGSRAFSLVGTAARLAINLLIALFGLYYAFLQPG
                     ETWKAVQPYIPFSVPNTDRLRDRFRNVTISTVIGTGATAALQGVMVGGAFWVAGLSNA
                     TFWGVVATVLAVLPVVGSGLVWAPGAVALTLDHRGGDAVLMALWGLVVVGNVGYIIQP
                     MVYRKYAHIHPLVTLLGALAG"
     gene            complement(877..2997)
                     /locus_tag="VFU75_10405"
     CDS             complement(877..2997)
                     /locus_tag="VFU75_10405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467293.1"
                     /translation="MIHLRTLGAVELTDGRGNPLDTVATQSKRFAILVYLALALPRGW
                     HRRDSLLALFWPELDQARARHALRQALHFLRKSLGDDVIRTRGSEDVAAGPALRCDAV
                     EFEDGDATAHTLALYRGDLLPGFFIRDAAPEWDHWMDERRNALRTRAEHLTERLGRPE
                     AMPPVAAPARRAGLRWRLPGLAAAAAFVAIVVGAWLVPQRALARSTLADSLYRRGLTT
                     LQAQGDRRAALGWFEAALEASPRFPLAAYYAGVTADQIDEQASRAWFTRAEAMIPQAS
                     EHDRLVIETAAAFRRDAPAALAIADSFARRYPTDPLAHHLLGASQLWAGDFAGALGEF
                     RTERAMVPESRDAQDGIVAALQFLDSLPSAEREAWVEVHSAPQSFAAWGSLAVIQAAE
                     GHTNNAKASARRSAELTPGHVDDPLSAADFEIRASHFAAADRMLRDGISYGTNAGREQ
                     ARWLLIISLRNQGRLQEALRYARAYRRENEDPSGLGAIPEAQVLFELGRTDEAGALFD
                     SIAAPRTVPRAQASGVDARRESWNLAHAAEAWAAAGDTTRVKAYADTIEGMARRSLYG
                     RDRHLPSHLRGLVWLARGDTDSASVALHAAVFSLSIGYTRTNLELARVLLDRGQAREA
                     ASVASAGLAEAVDGSAYYATRTELHELAARAFDAARQPDSAAAHYAAVVRAWGQSDPM
                     FAPRLGAARDRLAALQARGARGVD"
     gene            3123..4229
                     /locus_tag="VFU75_10410"
     CDS             3123..4229
                     /locus_tag="VFU75_10410"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HEU5467294.1"
                     /translation="MHPIALILSFLALGSGPTRHAAPKPMTRTYYIAADTVRWDYVPG
                     GINQLSGKAYQDTAFFPDSAPRAVSTQYLKILYREYTDSTFRTLKPRSPEWRHLGFLG
                     PLIRGVVGDTIRVVFRNNAPGRFSMHPHGVFYTKSSEGAPYVDGTSGADTADDGVPQG
                     GTHVYVWPVPARAGPGPNDGSSVLWMYHSHTDEVRDINSGLIGPMIITARDRARADGT
                     PMDVDREIVTAFAQVEENSSWFAERNLPAFAHRAIPRNVPGQFDAYPYFVEFTINGYT
                     HGSLPLDDLTLKKGQRVRWYVFGSTNDFDFHAPHWHGNTVLLNGMRTDVLQISMMQMV
                     VADMVPDNVGTWLFHCHVSFHFTDGMGVRYRVIE"
     gene            4386..5966
                     /locus_tag="VFU75_10415"
     CDS             4386..5966
                     /locus_tag="VFU75_10415"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU5467295.1"
                     /translation="MFGIVATLVPSFGIAYSSYVQNKRVVRDKIAQELATLAGPGARE
                     VEIWLKSHLLDLRVFAGSEILSGARGGTEKRRTDYLESVRAHFADYEALAALDPQGHA
                     IAISSRGAKSIPLPDDWGSMLRNSGAVIGEPFRTASGHIQLVLAVPIQTPTGRANNSL
                     AAIVDLKALTDNLSRFVPRDSGRFYVTTGTGLLVAGKGPLADSALKAQLPAATIQNLT
                     ARPGQALEYTGFGRKGVLGTMEPIDRLHWAAIAEVPATAAFRQIEHLRNVTTFVVAVI
                     FVSVGLMAYALGLLIVHPLDRLSEGAATVARGNLTVDLPVVGGGEVGRLTEVFNHMVG
                     RIREGRDALQKLSVTDGLTGLYNRRRLMETLDVEAERSERGAGPFSVLMVDVDHFKKY
                     NDTHGHQAGDAVLTRVAGILKEITRQIDCAARYGGEEFLVLLPQTAMDGAAEVAERLR
                     SQLKASGANGGGDAVTVSVGVAEFPKNGDTTQSVIAAADAALYRAKADGRDRVVRSAV
                     SAKPTRTSGASARRRRDG"
     gene            complement(5938..6666)
                     /locus_tag="VFU75_10420"
     CDS             complement(5938..6666)
                     /locus_tag="VFU75_10420"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HEU5467296.1"
                     /translation="MTLRNAGIGLVFLGVAACQKPATQPDPKIAADTGHQAPARDPEQ
                     DRRIAQLELQNMERDAEVEALQQQLDDARAEVVRAMAKVSTLATRAEAASNLAEAQVA
                     VQTLKTNAGTAPLPELASANQMLRQASTEFDKGNYGGVVYLSNQAKNFASEGQTRLSS
                     VDRSTLRAGEIPFAIPLSLHTTGHANIRSGPGTTFKVAYTLESGADVTAFSYVDEWVR
                     ITDSGDRTGWIHRTLISRRAAGAR"
     gene            6712..7422
                     /locus_tag="VFU75_10425"
     CDS             6712..7422
                     /locus_tag="VFU75_10425"
                     /inference="COORDINATES: protein motif:HMM:NF001911.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEU5467297.1"
                     /translation="MPAQGTPKNAGPIHVTWLGHATFLVTSAGGTSFLIDPFIKGNPA
                     TPDSLKDLTRYRPAAVIVTHAHSDHAGDVVAIAQASGAPVIGEDDFVNSLGLAETQVK
                     GGNVGGSFVVGDVTVHLVPAMHSSAPAGRPLGVVLTFADGRSLYHTGDTWIFGDMALI
                     EEIHHPSIILLNVGGGPYTQDPATAALAIKKYFHPTVIVPMHHRTFPGLATEDDVKKA
                     FAGDSRLHLLTVGQQATF"
     gene            7419..8081
                     /locus_tag="VFU75_10430"
     CDS             7419..8081
                     /locus_tag="VFU75_10430"
                     /inference="COORDINATES: protein motif:HMM:NF040120.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6498-containing protein"
                     /protein_id="HEU5467298.1"
                     /translation="MNGPRLTPSAFVLILANAVPILGVVFLHWTVFSVILLYWTENVI
                     IGGFNVLRMLTADPGDGVVWVGKAFLIPFFCIHFGMFTFIHGQLVLAFFGGEAFRRSS
                     FHVAGLIQAVGQAGIGYGVLFLVLSHTFSFIHDWLVSGEYRRASIRLLMGQPYSRILV
                     LHFTILFGGFLVQLAGAPVLAVVLLVTLKTAIDLRAHQTERQKLALAPVAERSERGAS
                     SG"
     gene            8065..9051
                     /gene="mgrA"
                     /locus_tag="VFU75_10435"
     CDS             8065..9051
                     /gene="mgrA"
                     /locus_tag="VFU75_10435"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047834 - D-threo-aldose 1-dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HEU5467299.1"
                     /translation="MHRAAESRYDGMPYQRCGRSGLKLPAIALGLWHNFGRVDQFENA
                     RGMLLHAFDRGITHFDLANNYGPPPGSAEETFGKVLREDLAPYRDEMIISTKAGYRMW
                     PGPYGEWGSRKYLLSSLDQSLKRMGIAYVDIFYHHRPDPDTPLEETMGALAQAVRSGR
                     ALYAGISSYTPEQTRRAAALLRDLGVPCLIHQPKYSMLVRGPENGLFQALKEEGIGCI
                     PFSPLAQGLLTDRYLKGIPEDSRAAKATGFLRREEVTDQRIEQARKLNDVAKARGQTL
                     AQLALAWVLRQPVVTSALIGASRIAQIDDAIGALGKRAFSAEELAAIDRILG"
     gene            9189..10460
                     /locus_tag="VFU75_10440"
     CDS             9189..10460
                     /locus_tag="VFU75_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467300.1"
                     /translation="MILPLLLLLQNPASPATDRPIRIWMDAAGPVSRGRSVRLYIQAG
                     SAGNLVVLHSRTDGKIEVLFPARPTDDPHVSPGMWEVRGKGDGPIWTVNEPDGSGMIL
                     AALSPDPVWFDEFSHEVSWNPDALRPSWDGADPAGGMEDIVQRMLGDGGFTYDVLNYT
                     VAPEAIAQGPGMYQSPQSVQNPDDTFPTIDNTLPEATKVCDGSNPYVYCSYLNEPLFG
                     GHHHRRLAQANPAPALPTPANPQGVASMVLPLHSAPAPASGGPVVPRRRMVPDVTVRS
                     RAPGPPAPTPGSSAVRTLASRPVTGAELGRLGGASPAARSNLILPYVHPPRPERQGPE
                     GATATIAPAAMAPPVERAAPATVAASMPIRSRGTMLMSRAVSPSAGAGVTRSAPPRTT
                     APGVAARPTAGSTAPRTGGTVGGWMVMPRRR"
     gene            complement(10465..11160)
                     /locus_tag="VFU75_10445"
     CDS             complement(10465..11160)
                     /locus_tag="VFU75_10445"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5467301.1"
                     /translation="MAEPLPVVLVVEDDPRMQKFLQTALVQHRYRPVEASTAREALTL
                     HAGHHPDVILLDLGLPDGDGIDVARTIRAASQTPIIILSARGQEGDKVSALDAGADDY
                     LTKPFGVEELLARLRVALRRAVRPANGGDVFGQFGEVRVDLEKREVRRGKEVVHVTPT
                     EFRILVTLVRHPGRVLTHRQLVREVWGVHAGDQTASLRVHMTQLRHKLEKDPARPRHL
                     TTEIGVGYRLQVD"
     gene            complement(11169..13835)
                     /locus_tag="VFU75_10450"
     CDS             complement(11169..13835)
                     /locus_tag="VFU75_10450"
                     /inference="COORDINATES: protein motif:HMM:NF014728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HEU5467302.1"
                     /translation="MTDGRPDPESLLARARAEERRVGRGRLKIFFGASPGVGKTYAML
                     EEARAKKREGVDVVVGLVETHGRRETAALLGGLDVLARRASEYRGVRLEEFDLDGALA
                     RHPGLILVDELAHTNAPGSRHTRRFQDVEELLAAGINVYTTLNVQHLESLNDVVAQIT
                     GVQVRETVPDSILDEADDVELADLSAEDLLTRLREGKVYVPEQAARALEEFFRKGNLI
                     ALRELALRRTAQRVDAQMRGYREEHGIQEIWPAADRLLVCIGPNPAGARLIRAGKRMA
                     SSLQCDWLVVSVEVPGQAISDSDRAALAQNLHLAQELGAQTATLSGHDVATEILAYAR
                     EQNVAKVVIGKPTHSRWRDKLFGSTLDALVRGSGDLEVHVISGDEVLPRAGRQRPAVH
                     RSPAAEYGWATLVVALGTALGAVVFRDLSVTDVAMVYLLGAGLVASRYGRGPSLFAAI
                     LSIACFDFFFVPPYFTFAVTDLRYLLTFGVMLAISVVISGLTLRIRDQAEAARDRERR
                     TGALYAMSRELAGARGREEVLRIVGHHVRASFAAEVYVLLPDGAGRLAVPPRVPLDEK
                     ERSVAQWVFDRGRPAGRSTDTLPAAHALYLPLVAASGGIGALGVSPSDAAGLDDVMVI
                     RLLEAFAGQAALALERELLAERAQREQLEVEAERLRTSLLSSLSHDLRTPLAAITGAA
                     GSLLDEHAPLAPAARRELLGSILEESQRMNRLIGNLLDMIRVESGALRIQREWQPLEE
                     PLGVALIRLGDRLKGREVKVGDLPLVPGDPVLLEQVFLNLIENAVKYTPPGSPIDISA
                     TSDGHVVRVAVADRGPGIAPGDAERVFEKFYRSAGAPAGGGVGLGLTICRGIVTAHGG
                     RIWVENRPGGGAIFQFTLPLSGPDIPAPPVIE"
     gene            complement(13846..>14842)
                     /locus_tag="VFU75_10455"
     CDS             complement(13846..>14842)
                     /locus_tag="VFU75_10455"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU5467303.1"
                     /translation="MYSTMTPRNDASPARGQWNLADAYRYISEAYGGFHIDHWNGVNV
                     EAGIFMSYVGLFSYHNYDNWAYQPSYVSSNTPWFFNGIRIQIFPSDKLKIEPWIVNGW
                     QSYGMFNSAPGLGWQVLWRPNATLSVLSNEYYGHDWLGIPKRWRVHSDNSLQIKYRDV
                     PGASVDKAAFSLTVDLGCESGGGVRCEGRGAGGPAQNFAGCMLYNRLWFAHDLYAVTL
                     GGGAMTNPGRYLVLMPPINGATAFSGTPYFSENPGDQFGAWDVSATFDYMPDQFATFR
                     MELDHRAATVPYFSGAGGVTPVGGNQGAPGSTVAGFQPDLRKSETRLNVAVLIKL"
BASE COUNT         2473 a         4841 c         5139 g         2389 t
ORIGIN      
        1 ccggcgagtg cgccaaggag cgtcacgagg gggtggatat gggcgtattt cctgtagacc
       61 attggctgga tgatgtatcc gacattgccg acgacgacga gtccccagag agccatcagg
      121 acggcgtcgc cgccgcgatg atcgagcgtc agcgcgacgg caccgggtgc ccagaccaag
      181 ccactgccga ccacgggtaa caccgccaac acggtcgcga ccactcccca gaaggtcgca
      241 ttcgagagcc ccgccaccca gaaggcccct cccaccatca cgccctgcag cgccgccgtg
      301 gccccggtgc cgatgaccgt cgagatggtc acgttgcgga aacggtcgcg caaccggtcc
      361 gtgttgggca cagagaaggg gatgtacggc tgaacggcct tccaggtctc tccgggttgc
      421 aggaaggcgt agtacaagcc gaagagcgcg atgaggaggt tgatggcgag ccgggcggcc
      481 gtgccgacca gtgagaatgc cctcgatccg atccaccccg cgacgtcctg cgtcatgctc
      541 gcgagctgag ggcccaggtc cgtgtcgccg atcttcaagt gggccagggc ggcgaggagt
      601 ggattcgaag ccccacctgc cacgacttgc tgcgcctggc cgacgaccag cgagacgaag
      661 gacaccccgg ggacgaccac caggaacagc gcgagcagca cgacgatcac cgcggcgggg
      721 gtcggccgga cgtgacgggc cagccggtca tgaaccggct ggaggatgac gtagagaacg
      781 gggatgccga tgaggccggt ggcgaacggg gtcagggcaa tcgccaaccc gactccgagg
      841 aggaggatga ggatggcggc gcgagtgtga ttcgagtcaa tcgacgccac gggccccccg
      901 ggcctgaagg gcggcgaggc ggtcgcgagc cgcgccgagg cgcggggcga acatcgggtc
      961 ggactgtccc caggcgcgaa ccacggctgc atagtgcgcc gctgcgctgt cgggttgtcg
     1021 ggctgcgtcg aaggcccgcg ccgccagctc gtgcagctcc gtgcgagtgg cgtagtacgc
     1081 cgatccgtcg acggcctccg ccagccccgc cgatgcgacg gacgcggcct ctcgagcttg
     1141 cccgcggtcc aggagcaccc gcgccagttc caggttggtt ctggtatagc cgatgctcag
     1201 tgagaacact gccgcgtgca gggccaccga cgccgaatcc gtatcgccgc gcgcgagcca
     1261 gaccagccct cggagatggg acgggagatg gcgatcccgg ccgtagagac tccgccgggc
     1321 catcccctcg atggtgtcgg cgtaggcttt gacgcgggtg gtgtctcccg ccgcggccca
     1381 tgcttctgcc gcgtgcgcca ggttccacga ttcgcggcgg gcgtcaacgc ccgacgcctg
     1441 cgcccgtggg acggtgcggg gcgccgcgat ggagtcgaag agggctcctg cctcgtcggt
     1501 acgtccgagc tcgaagagca cctgcgcttc ggggatggcg ccgagtcccg acgggtcttc
     1561 attctcacgc cggtaggcac gggcgtagcg caacgcttcc tggagcctcc cctgattccg
     1621 caggctgatg atgagcagcc accgggcctg ctcccgtccg gcattcgtgc cgtacgagat
     1681 gccgtcccga agcatccggt cggccgccgc gaaatggctg gcccggatct caaagtcggc
     1741 ggcactcaga ggatcgtcga cgtggccggg cgtcagctct gcggaccgcc gcgccgaggc
     1801 ctttgcgttg ttggtgtgtc cctcggccgc ctggatgacg gcgagggacc cccaggccgc
     1861 gaaggactgc ggggccgagt ggacctcaac ccaggcttcg cgttccgccg aggggaggga
     1921 gtcgagaaac tggagcgcgg ctacgattcc gtcctgggcg tcgcgggact cagggaccat
     1981 agcccgctcg gtccggaact cgcccagggc gccggcgaaa tcgcccgccc agagttgcga
     2041 cgctccgagg aggtggtgcg cgagcgggtc ggtcggatac cgtcgggcga aggagtccgc
     2101 gatggcgagg gccgcgggtg cgtcccggcg aaaagccgcg gccgtctcga tcaccagtcg
     2161 gtcatgttcc gacgcctgcg ggatcatcgc ttcggcgcgc gtgaaccacg cccgggaggc
     2221 ctgctcgtcg atctggtcgg cggtgacccc ggcatagtag gcggccagcg gaaagcgcgg
     2281 agatgcctcc agggcggcct cgaaccagcc cagggcggcg cgacggtccc cctgggcttg
     2341 aagggtggtc aggccgcggc ggtagagcga gtccgccagg gtggatcggg ccagcgcccg
     2401 ctggggcacg agccaggcac cgacgacgat ggcgacgaag gccgcggcgg ctgccaaccc
     2461 cggcaggcgc catcgtagac cggcccgacg ggctggagcg gcgacggggg gcatggcttc
     2521 gggtcgaccc aggcgttccg tcaggtgctc ggcccgggtc cgcagcgcgt tccgtcgttc
     2581 atccatccag tgatcccact ccggcgcggc gtcccgaatg aagaaccccg gcaggaggtc
     2641 gccacgatag agcgcgaggg tgtgggccgt ggcgtcacca tcctcgaact ccacggcatc
     2701 gcaccggagc gccgggccgg ccgcaacgtc ttcggaaccc cgggttcgaa tcacatcgtc
     2761 gcccaggctc tttcgcagga agtgcagtgc ctggcggagc gcgtgacggg cccgggcttg
     2821 atcgagctcg ggccagaaga gcgccaggag cgagtcacgg cgatgccagc cgcggggaag
     2881 agcgagggcg aggtagacga gaatggcgaa ccgtttggac tgggtcgcca cggtgtcgag
     2941 ggggttcccg cgaccatcag tgagctcgac ggctccaaga gtccgcagat ggatcatcgg
     3001 tcctgctcct cgttcgccag ggcggtgacg gctcggtgac ggcgggtgaa caccatgtcg
     3061 ggagatagcc taccgcgctc tttgaggcgc ggcaactcac ccacccgctc cgggaacccg
     3121 ccatgcaccc aatcgccctg atcttgtctt ttcttgccct cgggtccggt cccacacggc
     3181 acgcggcgcc caagcccatg acccgcacgt actacatcgc cgcggacacg gttcgctggg
     3241 actacgtgcc cggcggcatc aatcagcttt cggggaaggc gtatcaggac acggcattct
     3301 tcccggacag cgctccccga gcggtgtcga cgcaatacct caagatcctg taccgggagt
     3361 acacggacag cacattccgg acgctcaaac cccggtcacc ggagtggcgg catctggggt
     3421 tcctggggcc gctgattcgc ggagtggtgg gcgataccat ccgggtggtg tttcggaaca
     3481 acgcgccggg ccgcttctcg atgcacccgc acggcgtctt ctataccaag agctcggaag
     3541 gcgcgccgta tgtcgacggc acctcgggcg ccgacacggc cgatgacggc gtcccgcagg
     3601 gtggtacgca tgtgtatgtg tggccggtgc ccgcgcgcgc gggtccgggt ccgaacgacg
     3661 gttcgtccgt gctctggatg tatcactcgc acacggacga ggtgcgcgac atcaactcag
     3721 ggctgatcgg gcccatgatc atcacggccc gggaccgcgc ccgcgcggac ggcacgccga
     3781 tggacgtcga tcgcgagatc gtcaccgcat tcgcccaggt ggaggagaac tccagctggt
     3841 tcgcggagcg gaacctccct gcctttgcgc accgggccat tccccggaac gtgccgggcc
     3901 aattcgatgc ctatccgtac ttcgtcgagt tcacgatcaa cggatacacg catgggtctt
     3961 tgcccctgga cgacctgacc ctcaagaaag ggcagcgggt gcgctggtat gtgttcggat
     4021 cgacgaacga tttcgatttc cacgccccgc actggcacgg gaacaccgtc ctgctgaacg
     4081 gcatgcgtac ggacgtgcta cagatctcga tgatgcagat ggtggtggcc gacatggttc
     4141 ccgacaacgt ggggacgtgg ttgttccatt gtcacgtgag cttccatttc acggacggca
     4201 tgggggtccg gtaccgcgtg attgaatagg cggaatgggc accgggcggg cctgcttgca
     4261 tctatggcgc tttccccaat cttgttaggg tcccgtccgg agcctggtca ctgagatcct
     4321 gggaacaagt cgccgcccgt gtccttcggg cggtcggtct ggagagcatc cagcgcaagc
     4381 tgctggtgtt cggcatcgtc gcgaccctgg tgccgtcgtt cggcatcgcg tactcctcgt
     4441 acgtccagaa caagcgcgtg gtgcgggaca agatcgcgca ggagctcgcg acgctcgcgg
     4501 gccccggcgc ccgcgaggtc gagatctggc tcaagtcgca cctgctggac ctccgggtgt
     4561 tcgccggctc ggaaatcctg tcgggcgcgc gcggcggaac ggagaagcgg cgcaccgact
     4621 acctcgagtc ggtccgcgcg cacttcgcgg actacgaggc gctcgccgcg ctggaccccc
     4681 agggccacgc catcgccata tcgtcgcggg gcgcaaagtc catcccgctg cccgatgatt
     4741 gggggtcgat gctccggaat agcggggccg tgatcggcga accgttccgg accgcttcgg
     4801 gccacatcca gctcgtcctc gcggtcccga tccagacgcc cacgggccgg gcgaacaaca
     4861 gcctggcggc catcgtcgac ctgaaggccc tgacggacaa cttgtcccga ttcgtgccga
     4921 gggactccgg ccggttttac gtgacgaccg gcaccggcct cctggtcgcc ggaaagggac
     4981 ctttggcgga ttcggctctc aaggcacagc tcccagccgc gaccatccag aacttgacgg
     5041 cccgtccggg ccaggcgctc gagtatacgg ggttcggacg gaagggcgtc ctgggtacga
     5101 tggagcccat cgatcggctg cattgggccg cgatcgccga ggtgcctgcg acggcggcgt
     5161 tccgccagat cgagcacctg cgcaacgtca cgactttcgt cgtggccgtc attttcgtca
     5221 gcgtcggcct gatggcgtac gcgctgggat tgctcatcgt gcacccgctc gaccgcctgt
     5281 cggaaggcgc ggcgaccgtg gcgcggggga acctgaccgt cgatctgccg gtggtgggtg
     5341 gcggtgaggt cggcagactc acggaagtct tcaatcatat ggtggggcgg atccgcgagg
     5401 gccgtgacgc gctccagaaa ttgtcggtga cggacgggct gaccgggctc tacaaccggc
     5461 ggcgcctgat ggaaacgctg gacgtcgagg cggagcggtc ggagcggggc gcgggtccgt
     5521 tctcggtgct gatggtggat gtcgatcact tcaagaagta caacgacacc cacgggcatc
     5581 aggcgggaga cgcggtgctc acccgggtgg ccggaatcct gaaggagatt acccgtcaga
     5641 tcgactgcgc ggcgcgctat ggtggggaag agttcctggt gctcctcccg caaaccgcga
     5701 tggatggcgc cgcggaggtc gcggaacgtc tgcgttcgca attgaaggca tccggcgcga
     5761 atggcggggg ggatgcggtg acggtcagcg ttggcgtcgc ggaattcccc aagaacggcg
     5821 atacgacgca gtcggtgatc gccgcggcgg acgccgcact gtaccgggcg aaagcggatg
     5881 gccgggaccg ggtggtccgt tcggcggtgt cggcgaagcc gacgaggacg tcgggggcta
     5941 gcgcgcgccg gcggcgcgac ggctgatcag ggtccggtgg atccagcccg tgcggtcccc
     6001 tgaatcggtg atccgaaccc actcatccac gtaggaaaac gcggtgacgt cggcgcccga
     6061 ttccagggtg taggcgactt tgaacgtcgt gcccgggccc gagcggatgt tcgcgtgacc
     6121 tgtggtatgc agggacagcg ggatggcgaa ggggatctcg cccgcacgaa gggtcgagcg
     6181 atcgacactc gacaggcggg tctggccctc agacgcgaag ttcttggcct gattcgagag
     6241 gtacaccacg ccgccgtagt tccccttgtc gaactccgtc gacgcctggc gcagcatctg
     6301 attcgcggaa gccagctccg gcaggggcgc cgtgcccgcg ttggtcttca aggtctgcac
     6361 cgccacctgg gcctccgcca gattggaggc cgcctcagca cgggtcgcga gggtcgacac
     6421 cttggccatc gcccgcacga cttcggcgcg ggcgtcatcg agctgctgct gcaacgcctc
     6481 gacttcggcg tcgcgttcca tgttctgcag ctcgagctgc gcgatccggc gatcctgctc
     6541 gggatcacgg gcgggcgcct ggtgaccggt gtcggcggca atcttggggt cgggctgggt
     6601 cgctggtttc tgacacgcgg ccactcccaa aaacaccagt ccgattccag cattccgtag
     6661 cgtcatagtg gcaaaaatat caccctcatt cacggtcgcc gggagtacca aatgcctgct
     6721 cagggaacgc ccaagaacgc cgggccgatc cacgtgacgt ggctgggtca tgccacgttc
     6781 ctggtcacca gcgcgggcgg gacgagcttc ctcatcgacc cattcatcaa ggggaatccg
     6841 gcgacacccg attcacttaa ggatttgacc cggtaccgtc ctgccgcggt catcgtgacg
     6901 cacgcccata gtgaccacgc gggcgacgtc gtggccatcg cgcaggcgag cggagcgccc
     6961 gtcatcgggg aggacgactt cgtcaactcg ctcgggctgg cggagacgca ggtgaagggg
     7021 ggcaacgtgg gcggatcgtt cgtggtcgga gatgtgaccg tccatctcgt gcccgccatg
     7081 cactcgagcg ccccggcggg tcgcccgctt ggcgtggtgc tgacgttcgc ggacggccgc
     7141 tcgctctacc acaccggcga cacctggatc ttcggggaca tggcgctcat cgaggaaatc
     7201 catcacccca gcatcatcct gctcaacgtc ggcggcgggc cctacacaca ggatccggcc
     7261 actgccgcgc tggcgatcaa gaagtacttt cacccgacgg tgatcgtgcc catgcaccac
     7321 cggaccttcc cgggactcgc gacggaggac gacgtgaaga aggcgttcgc cggcgactcg
     7381 cgactccatc tcctgacggt tggacaacag gcgaccttct gaacggaccg cgtctcaccc
     7441 catccgcgtt cgtgctgatt ctggcgaatg cggttcccat cctcggggtg gtgtttctcc
     7501 actggaccgt gttttcggtg atactgctgt attggaccga gaacgtgatc atcgggggat
     7561 tcaacgtact gcgaatgctg accgccgacc ccggggatgg cgttgtatgg gtgggcaagg
     7621 cgttcctcat cccgttcttc tgcattcact tcgggatgtt caccttcatt cacggccagc
     7681 tcgtcctcgc ctttttcggt ggcgaagcct ttcgacggag ctcgttccac gtcgccgggt
     7741 tgatccaggc agtcgggcag gcgggaatcg ggtatggtgt cttgttcctg gtgctgtcgc
     7801 ataccttttc cttcatccac gactggctgg tgagtggtga ataccggcgc gcatccattc
     7861 gcctgctgat ggggcaacca tattccagaa tcctcgtctt gcatttcacg attctgttcg
     7921 gcggattcct ggtgcaactc gccggagccc cggttctcgc cgtcgtgctt ctggtgacct
     7981 tgaagacggc tatcgacctg cgagcgcatc agaccgaacg gcagaagctg gccctggccc
     8041 ctgtggccga gaggagtgaa cgcggtgcat cgagcggctg agagccgata cgacggcatg
     8101 ccctaccagc gatgcgggcg cagcgggctg aagctgcccg ccatcgcgct cgggctctgg
     8161 cacaacttcg gccgtgtgga tcagttcgag aacgcgcggg gaatgctgct tcacgcgttc
     8221 gatcgcggca tcacgcactt cgatctcgcg aacaactacg gaccaccccc ggggtccgcc
     8281 gaggagacgt tcggcaaggt gctgcgggag gacctcgcgc cataccggga tgagatgatc
     8341 atctccacca aggcaggata ccggatgtgg cccggaccgt acggcgagtg gggatcacgg
     8401 aagtacctgc tctcgagtct cgaccagagc ctgaagcgga tgggcatcgc gtacgtcgac
     8461 atcttctatc atcatcggcc cgatcccgat acgcctctcg aagagacgat gggcgcactg
     8521 gcgcaggcgg tgcggagtgg aagggccctc tacgccggga tctcgtcgta cacgcccgag
     8581 caaacccgac gagccgcggc cctgctccgg gatctcggcg ttccgtgcct gattcaccag
     8641 cccaagtaca gcatgctggt ccgcggtccg gagaacggac tctttcaggc cctcaaggag
     8701 gagggaatcg ggtgcattcc gttttctccg ttggcgcagg ggctgctcac ggaccgctat
     8761 ctcaagggga ttcccgagga ctcccgggcg gccaaggcga ccggattcct ccggcgtgag
     8821 gaagtcaccg accagcggat cgaacaggcg agaaagttga acgacgtcgc caaggcgcgc
     8881 ggtcagacgt tggcgcagct cgccctggcc tgggtcctgc gccagcccgt ggtcacctcg
     8941 gcactcatcg gggcgagccg gatcgcgcag atcgacgacg cgatcggcgc cctcggcaag
     9001 cgagccttca gcgccgaaga gttggctgcg atcgacagga ttctgggcta gctgcggcca
     9061 gggtggcggg caggagcacg agcgtaatgt caggaaatgc gcacacagca ggaatgcgcg
     9121 gagtggcggg gaacggctga ccacctggga gcgtatgcag tatgatacgc gaccagtccg
     9181 gaggccacat gatcctgccg ctcttgctcc tccttcagaa cccagccagt ccggcgacgg
     9241 accggccgat tcgcatctgg atggacgccg ccgggcccgt gtcccggggg cggagcgtcc
     9301 ggctgtacat ccaggcgggc tcggcgggca acctggtggt gctccactcg cggacggacg
     9361 gcaagatcga ggtgctgttc ccggcgcggc ccaccgatga cccacacgtt tcgcctggca
     9421 tgtgggaagt gcggggaaag ggtgatggac cgatctggac cgtgaacgag cccgacggca
     9481 gcgggatgat cctcgcggcg ctgagtcccg acccggtgtg gttcgatgag ttctcgcacg
     9541 aggtctcgtg gaatcccgac gccctcaggc cgtcgtggga cggggcggat ccggcgggtg
     9601 ggatggagga catcgtccag cggatgctgg gagacggcgg atttacgtac gacgtcttga
     9661 attacaccgt ggcccccgaa gccatcgcgc aggggcccgg catgtatcaa agcccgcaga
     9721 gcgtccagaa ccccgacgac acctttccga ccatcgacaa cacgcttccc gaagcgacga
     9781 aggtgtgcga cggatccaac ccgtacgtgt attgctccta tctcaacgaa ccgctcttcg
     9841 gcggccacca tcatcgccga ctggcgcagg cgaaccccgc gcctgcgttg ccgactccgg
     9901 ccaaccccca aggcgtcgcc tcgatggtgt tgccgcttca ctcggcgccg gctccggcct
     9961 cagggggccc ggtcgtccca cgacgcagaa tggtgcctga tgtcacggtc aggagccggg
    10021 caccgggtcc gccggctccc actcccggat cgagtgcggt gcggaccctg gcgtcgcgtc
    10081 cggtgacggg tgcggagttg gggcggctgg ggggagcgtc gcccgcggcc cggtccaacc
    10141 tgatcctccc gtacgtccat ccgccgcgtc cggaacggca ggggcccgag ggggcgacgg
    10201 cgaccattgc gcccgccgcc atggccccgc cagtcgagcg tgcggcgccg gccacggtcg
    10261 ccgccagcat gccgatccgc tcgcgtggta cgatgctgat gtcgagggcg gtaagcccga
    10321 gtgccggagc gggtgtgact cggagtgcgc cgccgaggac gacggcgccc ggcgtggctg
    10381 ctcgtcccac ggccggctcg acggcgcccc gcaccggcgg cacggtcggc ggctggatgg
    10441 taatgccgcg gcggcgctag accgtcagtc gacctgaagc cggtacccga cgccgatctc
    10501 ggtggtgaga tgccggggac gggcagggtc tttctcgagc ttgtgccgaa gttgtgtcat
    10561 gtgcacccgc aggctcgcgg tctggtctcc ggcgtgaaca ccccaaacct cccgaaccag
    10621 ttgccggtga gtcagcaccc gccctgggtg ccgcacgagc gtgaccagga tgcgaaactc
    10681 ggtgggcgtg acatgcacga cttccttccc gcgccggact tcccgcttct cgaggtcgac
    10741 gcgcacttcg ccgaactggc cgaacacgtc cccgccgttt gcggggcgta ccgcgcgtcg
    10801 cagggcgacc cgaagacgcg cgagcagctc ttcaacgccg aagggcttgg tgagatagtc
    10861 atcggcgccg gcgtccaggg ccgagacctt gtccccttct tgtccccgcg ccgacaggat
    10921 gatgatgggg gtctgggagg cggcgcggat ggttcgtgcg acgtcgatgc cgtcgccgtc
    10981 ggggagaccg aggtccagga ggatgacatc ggggtggtgg ccggcgtgca aggtcagggc
    11041 ctcgcgcgcg gtggatgctt ctacgggccg gtaccggtgc tgtaccagcg cggtctgaag
    11101 aaacttctgc atgcgtggat cgtcttccac cacgagcacg acggggaggg gctccgccat
    11161 gcccgaagct actcgatgac cggtggagcg ggaatgtccg gtcccgaaag cggaagggtg
    11221 aactgaaaga tggcgccgcc accagggcga ttttccaccc aaatgcggcc tccgtgcgcg
    11281 gtgacaatgc cgcggcaaat cgtgagtccg agtccaaccc cgccacccgc cggcgcgccc
    11341 gcggaacggt agaacttttc aaacacccgc tcggcatcgc ccggagcgat gcccggtccc
    11401 cggtcggcca cggccacccg gacgacatgt ccatccgatg tcgcgctgat atcgatcgga
    11461 gatccaggag gcgtgtactt caccgcgttc tcgatcaggt tgagaaacac ctgctcgagc
    11521 agcaccggat cccccggtac caggggcagg tcccccacct tcacctcgcg gcccttcagc
    11581 cggtccccca gccggatcag cgccactccc aggggctcct ccaggggctg ccactcccgc
    11641 tgaatgcgga gggctccgct ttcgacgcga atcatgtcca gcaggtttcc gatcaagcgg
    11701 ttcatgcgct gcgattcttc caggatcgag ccaaggagct cccgtcgcgc cgcgggcgcg
    11761 aggggcgcgt gctcgtccag caggctgccg gccgcacccg tgatggcggc gagaggcgtt
    11821 cgcagatcgt gcgacaagga cgacagcagg gacgtccgca ggcgctcggc ttccacctcc
    11881 agttgctccc gctgggcccg ctcggccagg agctcgcgct cgagcgccag cgcggcctgg
    11941 cccgcgaacg cctcgagcag gcggatgacc atcacgtcgt cgaggccggc ggcgtccgag
    12001 ggactgacac ccagcgcgcc gatcccgccg ctggcggcga cgagggggag gtagagggcg
    12061 tgcgcggccg ggagcgtgtc cgtggaccgg cccgccgggc ggccgcgatc gaagacccac
    12121 tgggccacac tcctttcttt ttcgtcgaga ggaacacgcg gcggcaccgc cagccgtccg
    12181 gccccatcgg gtagcagcac atagacctct gccgcaaagc tcgcccgcac atggtggccg
    12241 acgatacgca ggacctcctc gcgtccccgg gctccagcca gctcgcggct catcgcgtag
    12301 agcgcccctg ttcgccgctc gcgatcgcga gccgcctcgg cctggtcccg aatcctcagg
    12361 gtcaaccccg agatcacgac cgaaatggcg agcatgacgc cgaaggtcag cagataacgc
    12421 agatcagtga ccgcgaacgt gaagtaggga gggacgaaga agaagtcgaa gcaggcgatg
    12481 gacaggatgg cggcgaagag gctcggaccg cggccgtagc gggaagcgac cagccccgcg
    12541 ccgagcaggt ataccatcgc cacgtcggtg acggagagat cgcggaagac cacggcgccg
    12601 agcgccgtcc ccagcgccac gaccagcgtc gcccagccgt actcggcggc cggagagcgg
    12661 tggaccgccg ggcgttgcct gccggcgcgg ggcaagacct cgtcaccgga gattacatga
    12721 acctcgagat ctccggagcc gcgtaccagt gcgtcgagag tggatccgaa cagcttgtcg
    12781 cgccagcggg agtgcgtggg cttgccgatc acgaccttcg cgacattctg ctcccgggcg
    12841 tacgcgagga tctcggtggc cacgtcgtgc ccggagaggg tggcagtctg ggcgcccagc
    12901 tcctgggcca ggtggaggtt ctgagccagg gccgcgcggt cggagtcgga gatcgcctgt
    12961 ccgggcacct ccacggagac caccagccag tcgcattgga gactcgaggc catgcgcttg
    13021 ccggcgcgga tcaatcgggc cccggcgggg ttgggtccga tgcagacgag gagacggtcg
    13081 gcggcgggcc agatctcctg aatcccatgt tcctcgcggt agccccgcat ctgggcgtcg
    13141 acgcgctggg cagtccggcg cagggccagc tcccgcaaag cgatgagatt gcccttgcgg
    13201 aagaactcct cgagcgcccg ggccgcctgt tcgggcacgt acaccttgcc ttctctcagc
    13261 cgggtgagca ggtcctccgc cgagaggtcg gcgagctcga catcatcggc ctcatccagg
    13321 atggagtcgg gcaccgtctc ccgtacctgc acgcccgtga tctgggcgac gacgtcattc
    13381 aggctttcga ggtgctgaac gttgagggtc gtgtacacat tgatgccggc ggcgaggagc
    13441 tcctcgacgt cctggaaccg gcgggtgtga cgtgatccgg gggcgttggt gtgggccagc
    13501 tcgtcaacga ggatcaggcc ggggtggcgg gccaaggcgc cgtcgagatc gaactcttcg
    13561 aggcggacgc cgcggtactc ggaggcccgg cgggcgagga cgtccagtcc tccaaggagc
    13621 gccgcggtct cgcgacgccc atgagtctcg acgagtccca ccacgacgtc cacgccctcg
    13681 cgcttcttcg cgcgggcctc ttcgagcatc gcgtaggttt tccccacgcc gggggacgcc
    13741 ccgaagaaga tcttgagccg gccgcgaccc acgcggcgct cttccgcgcg cgcccgcgcg
    13801 agcagcgact cggggtcggg gcggccgtcg gtcatgggct gtaagctaga gcttgatcag
    13861 gacggccaca ttgagcctgg tttccgactt gcggaggtcg ggctgaaagc cggccaccgt
    13921 cgagcccggg gccccttggt tgccccccac cggcgtgacg ccccccgcgc cggagaagta
    13981 gggcaccgtg gcagcgcgat ggtcgagctc catgcggaag gtcgcgaact gatcgggcat
    14041 gtagtcgaag gtcgcggaca cgtcccaggc gccgaactga tccccggggt tctcgctgaa
    14101 gtacggagtg ccggaaaacg ccgtagcgcc gttgatcgga ggcatcagca cgaggtagcg
    14161 gccggggttg gtcatggctc cgccacccag ggtcacggcg tacaagtcat gcgcgaacca
    14221 gagccggttg tacaacatgc aaccggcgaa attctgcgcc gggccaccag cgccgcgccc
    14281 ctcacagcgt acgccaccgc cgctctcgca gcccaggtcg accgtcagcg agaacgccgc
    14341 tttgtcgacc gacgctccgg gaacgtcccg gtatttgatc tgcaggctgt tatccgagtg
    14401 gacccgccaa cgcttcggga tgcccagcca gtcgtgcccg tagtattcgt tggacaggac
    14461 tgacagggtg gcgttgggac gccagagcac ttgccagccg agcccggggg cggaattgaa
    14521 catgccgtat gactgccacc cgttcacgat ccagggctcg atcttgagct tgtcggaggg
    14581 gaagatctgg atgcggatcc cgttgaagaa ccagggcgta ttcgacgaga cgtacgacgg
    14641 ctggtaggcc cagttgtcgt agttgtggta actgaagagt cccacgtacg acatgaagat
    14701 gcccgcctcg acgttcacgc cgttccagtg gtcgatgtga aaaccgccgt aggcttccga
    14761 gatgtaccgg taggcgtccg ccagattcca ttgcccccgt gcgggcgagg cgtcgttgcg
    14821 gggcgtcatg gtcgagtaca tg
//
LOCUS       DASUBP010000278         8487 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_391419, whole genome shotgun sequence.
ACCESSION   DASUBP010000278 DASUBP010000000
VERSION     DASUBP010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 8487)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8487)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8487
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_391419"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..868
                     /locus_tag="VFU75_10460"
     CDS             <1..868
                     /locus_tag="VFU75_10460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467304.1"
                     /translation="RTVNDGTNSSSLRSSRFPMLQVAGPVVGHLVVGGGFSTYLYRSY
                     SVRVPGSVVIRGVPESYVDELGSDGAVTDLRLAAAWQFSPRLSVGGGLHILTGSSSLT
                     ATRLFTDSAVYKNASQADQESYDGFGVSLSVLAAPASGVRVTAFLQSYNHLRAVVNGF
                     QNAQNELPTTLGGAVQWSVTPEVKLAGSITHANWSVASATGTAFNTTNWAVGGELGRA
                     VPFRIGVRGGQMPFGPGPSAPTQFGISAGTGFTFSQGRGILDFGIEHLRRTGGGLTEN
                     GWNLLAGLTVRP"
     gene            898..2217
                     /gene="miaB"
                     /locus_tag="VFU75_10465"
     CDS             898..2217
                     /gene="miaB"
                     /locus_tag="VFU75_10465"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HEU5467305.1"
                     /translation="MKTRGVYIETYGCQMNVSDSELMLGLLTREGFAVVDSPSDADVL
                     LVNTCAVRDHAEQRVLSRMGELKAFKRPGGVLGVVGCMAQRLGPRLLERVPQVDLVVG
                     PDGYRNLPELIARAAAGTRAADVEFRGWEHYEDVPAQRADAVSAYVTVQRGCDYRCTF
                     CIVPMTRGPERSRKLADVVDEVRRLAAGGTTEVTLLGQTVNSYSDGTADFADLLRAVG
                     AVDGIRRVRFTSPYPTDFTHRVLAAMAETPAVCEHVHLPVQSGSNALLKRMLRRYTRE
                     RYLEVVEALRRAVPGLGLTTDIIVGFPGETEADFRDTLDLVETVGFDDAYTFKYSPRE
                     GTPAVKIKDAVPDDVAGERLERLVAVVRRLARTRNVGLVGTVHEVLVEGFAKRGGLLQ
                     TRTRGNKVVLVEGPESWIGTYTRVRLTGTTGSTFTGWPVERELSVVG"
     gene            2226..2495
                     /locus_tag="VFU75_10470"
     CDS             2226..2495
                     /locus_tag="VFU75_10470"
                     /inference="COORDINATES: protein motif:HMM:NF022179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late competence development ComFB family
                     protein"
                     /protein_id="HEU5467306.1"
                     /translation="MWEMAQREYDLLRPQLPQFCGCDTCRGDVLVFALNRLDPHYVST
                     RQGEVLTGLSLSTDQARAKTDVVLMEGFRKVAIAPRCGATPVQLP"
     gene            2516..4744
                     /locus_tag="VFU75_10475"
     CDS             2516..4744
                     /locus_tag="VFU75_10475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEU5467307.1"
                     /translation="MRAPIVLFALAFGAGLTAGLVPFLDPRSIPVTLVAAAVVGVTAS
                     RWYARAPRLALVVVVTAAGVLWGAGAQREQLWTCVGQWTGTKATHALVVRLLDPVSPG
                     GEVVGADPLEAGCEGEVPLRWPADSVAQGGTTWLIAGRWLSEGDGARGVLRVRFARVL
                     DATPRGRGGLRDAVSRRIHTLFGSRAPLVDALVIARRNALDPEVRERFAQAGLAHLLC
                     VAGLHVGFLAGWLAVLLRRLPLSPTARYLAGAVVVLGYVWLLGFPPPATRAGVGLAVA
                     GYARLRQELVPPVAVLALTALILLLFDPFSAHSVGAWLSFAAVGAVFWAIRATAGAPW
                     YVRLVAPGIAATLMTAPITALAFGTVAPIGVLVNLLAIPLAGVAIPGAMLAVIVTLLL
                     PPIGAMLARGAGLGLALLDALAGAGDRVPFGHLVMVAGPAAALTWLVVLVVAWWLWRA
                     PRRRWVIGARVALVAAIVAWTGAFRAQSLDDCRCLAISFLDVGQGDAAALRTPRGQWV
                     VIDGGPRAPGNDAGRRVVVPYLRRHGAHALALMISTHSDADHLGGLPAVVAAFPPRLV
                     IEPGEPLARPLYFEFLAAVEEAGSRWRPARAGDRIILDSVIIQVLSPDDDWMGVPVDV
                     NDHGVVVLVTYGTTRVLFQADAGVPVEERLAGTVGHVDLLKVGHHGSRSASSEDWLAE
                     LSPPVAVISVGARNRYGHPAPEVLERLARRGIRVLRTDQRGTITFTSDGHNAPRDIGH
                     HD"
     gene            4713..5738
                     /gene="fbp"
                     /locus_tag="VFU75_10480"
     CDS             4713..5738
                     /gene="fbp"
                     /locus_tag="VFU75_10480"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HEU5467308.1"
                     /translation="MHHGTSVITIERFIVDQERLHPEATGELSNLLYDLALAGKIISR
                     QVRRAGLVDIFGSAGSVNVQGEVQQKLDIFANETVRHSVQHTGRTCVMASEEDELPVP
                     VPEGKPLGKYVLLYDPLDGSSNIDVNVPIGTIFSIHRRRTRAGTPGTLDDCLQPGYSQ
                     VAAGYIVYGSSTMLVYTTGQGVHGFTLDPTIGEFLLSHPDIRSPGVGKYYSVNESNWN
                     RWPDPVHRVVAAFKTGEGPIQARNARYVGSLVADFHRNLLSGGIFLYPADAKSPQGKL
                     RLLYEACPLAMVVEQAGGAATDGRRRILEVEPRELHQRTPLIIGSKEDVEYATHVLST
                     EGAGARA"
     gene            5735..7252
                     /locus_tag="VFU75_10485"
     CDS             5735..7252
                     /locus_tag="VFU75_10485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HEU5467309.1"
                     /translation="MTWNGDPRRDLGAPGAFPFTRGIHASMYRGRRWTMRQYAGFGTA
                     EETNERFRHLLAAGQTGLSVAFDLPTQMGYDSDHAMAEGEVGRAGVAIDTVDDLERLF
                     HEIPLDKVSTSMTINATAAILLAMYVVVGEEQGVRREQLQGTIQNDILKEFIARGTYI
                     YPPEPSLRLIVDILRFTSDAGMKFNPISISGYHIREAGATAAQEIAFTFANGLEYVRR
                     AVAAGLDVNTFAPRLSFFFAGYTDLFEEVAKFRAARRLWARLLRERYQASDDACRLRF
                     HTQTGGATLTAQQPLNNVVRVALQAMAAVLGGTQSLHTNSYDEALALPTTQSATLALR
                     TQQVLANETGVPDVVDPLGGSYYVEALTDRLEGEARALLDQVEAMGGSARAIERGFFQ
                     DAIARSAYAFQRAVEAGEQVVVGVNKFEDDGTPPSIPAPDYSTLAAGQRERLKRARSQ
                     RNAAGATKAIQAVTAGAKDAGTNLMPVIIDAVRARCTVGEISDALRGVWGVYQPG"
     gene            7266..7493
                     /locus_tag="VFU75_10490"
     CDS             7266..7493
                     /locus_tag="VFU75_10490"
                     /inference="COORDINATES: protein motif:HMM:NF018595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HEU5467310.1"
                     /translation="MTERRRQTFQANGVEVTFDPDVCRHTGVCLRGLPLVFDIKRKHW
                     IRPENASPDDVIAQVARCPSGALQARRLEEK"
     gene            7490..>8487
                     /locus_tag="VFU75_10495"
     CDS             7490..>8487
                     /locus_tag="VFU75_10495"
                     /inference="COORDINATES: protein motif:HMM:NF014717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEU5467311.1"
                     /translation="MNLRRAFETARWAVRAYPGSTALLAGASAVTVGVTLPALALGAG
                     RLALPPATDPGLPLGFSSFAGTPATMQAQGIDAIAGLLVALGIAALAVGIVTAGSLAV
                     GRAAGRRSEISVRRAVGAARSSLRLSGLVEGVLLALGAIGVGLVPAVAAARWAVATWP
                     GTLLPALVGALAAIAAVGGVLLLGALFGVRAPGGTRVPYPGASSHGLVIPAAQFAISL
                     AILTGAARTGARATQLLHSGDPGVSGARAASVIAIENEASASVRATGYESLIRRLKAE
                     PGFGVVGLSSAGALMGMGPVDFVTTDCGQCREGLIYTPFRPVPAMIMAMTADTFRAA"
BASE COUNT         1184 a         2806 c         3065 g         1432 t
ORIGIN      
        1 tcggaccgtc aacgatggga cgaactcgag ctcgctgcgg agctcccgct tcccgatgct
       61 gcaggtcgcc ggcccggtgg tgggccacct cgtggtcggg ggaggcttct cgacctacct
      121 gtatcgctcc tattcggtcc gggtgccggg cagcgtcgtg atccgcgggg tgccggagag
      181 ctacgtggac gagttgggga gtgacggtgc ggtgaccgat ctccggctgg cggcggcctg
      241 gcagttcagt ccgcggctgt cggtcggcgg cggcctccac attctcacgg gctcgagcag
      301 cctcacggcg acgcggttgt tcacggactc cgctgtctac aagaacgcgt cgcaagccga
      361 tcaggagagt tacgacgggt tcggcgtctc gctcagcgtc ctcgccgcgc cggcgtccgg
      421 ggtgcgggtc acggcgttcc tgcagtcgta caaccacctg cgggccgtgg tcaacggctt
      481 ccagaacgca cagaacgagc tcccgaccac cctcgggggc gcggtgcagt ggtccgtgac
      541 cccggaagtg aagctcgccg gcagcatcac ccacgcgaac tggtcggtgg cgagtgccac
      601 ggggacggcg ttcaatacca ccaactgggc ggtgggcgga gaactcggcc gggccgtccc
      661 gttccgcatc ggcgttcgag ggggtcagat gccgttcggg ccgggtccct cggcaccaac
      721 ccagttcggc atttccgccg ggacggggtt caccttctcg cagggcaggg gcattctgga
      781 cttcgggatc gagcacctgc gccggacggg gggcggactc accgagaatg gctggaatct
      841 gctggccggc ctgaccgtcc gcccctgagg gcgtttcttc ctctatcttt acccgctgtg
      901 aaaacgcgcg gcgtctacat cgagacctac gggtgccaga tgaacgtgtc ggattccgag
      961 ctcatgctcg ggctgctgac ccgggaaggc ttcgccgtag tcgattctcc gtcggatgcc
     1021 gatgtgctcc tggtcaacac ctgcgcggtg cgcgatcacg ccgaacagcg cgtgctgtcg
     1081 cgcatgggtg agctcaaagc cttcaagcgg cccggcggcg tactgggagt cgtgggctgc
     1141 atggcccagc gcctgggacc gcgactgctc gagcgggtgc cgcaggtgga tctggtcgtc
     1201 ggccccgacg gctatcgaaa cctcccggag ctgatcgccc gtgccgcggc gggcacccgc
     1261 gcggccgatg tcgagttccg tgggtgggag cactacgagg acgtaccggc gcagcgcgcg
     1321 gatgccgttt cggcgtacgt gaccgtgcaa cgtggctgcg attatcgatg cacgttctgc
     1381 atcgttccga tgacgcgtgg accggagcgc agccgtaagt tggccgacgt ggtggacgag
     1441 gttcgccggc tggcggcggg cggcaccacc gaggtcacgc tccttggaca gacggtcaac
     1501 agctactcgg atggcacggc ggacttcgcc gatcttctgc gcgccgtggg cgccgtggac
     1561 ggaattcgcc gcgtccggtt cacgtcgccg tacccgacgg acttcaccca ccgcgtgctc
     1621 gcggcgatgg ccgagacgcc cgccgtctgt gaacacgtgc atttgccggt gcagagcggg
     1681 tcgaacgccc tgctcaagcg gatgctccgt cgctacaccc gggagcggta cctcgaagtg
     1741 gtcgaggcgc tgcgtcgcgc cgtgccgggc ctcggcctca cgactgacat catcgtcggg
     1801 ttcccggggg agactgaggc cgacttccgc gacactctcg atctggtgga gacggtcggc
     1861 ttcgacgacg cgtatacgtt caagtattcg ccccgcgagg gtacgccggc cgtcaagatc
     1921 aaggacgccg tgcccgacga cgtcgcggga gagcggttgg agcggctggt ggcggtggtg
     1981 cggcgtctcg cgcgcaccag gaacgtcggg ctcgtcggca ccgtgcacga ggtgctggtc
     2041 gaaggattcg cgaagcgcgg cgggctgctg cagacccgca ctcgcgggaa caaggtcgtg
     2101 ctcgtcgagg gtcccgagtc gtggatcggg acctacacca gggtgcgtct caccgggacc
     2161 actggctcga cgttcaccgg atggccggtg gaacgggagt tgtccgtcgt ggggtaacga
     2221 acgtgatgtg ggaaatggcg cagcgggaat acgacctgtt gcgccctcag ctcccccagt
     2281 tctgcgggtg cgacacctgc cgcggggacg tgctggtgtt cgccctcaac cggctggatc
     2341 cccactacgt gtcgacccgg cagggcgagg tgctcacggg tctctccctc tcgaccgatc
     2401 aggcgcgggc gaagaccgac gtcgtcctga tggagggctt ccgcaaggtc gcgatcgctc
     2461 cccgttgcgg cgcgaccccg gtgcaattgc cctagcctgc cgctaagggg ccccggtgcg
     2521 ggcgcccata gttctcttcg cgttggcatt cggggccggc ctcaccgccg gcctcgttcc
     2581 gtttctcgat ccccgcagca tccccgtcac gctcgtggcg gcggcagtcg tgggtgtcac
     2641 ggcgtcgcgg tggtacgcgc gggcgccgcg gctggcgttg gtggtggtgg tgacggcggc
     2701 gggagtgctc tggggtgccg gagcacagcg cgagcaactg tggacctgtg tcggtcagtg
     2761 gaccggtacc aaggccacgc acgcgttggt cgtgcggctc ctcgaccctg tctcaccggg
     2821 cggggaagta gtgggcgctg atccgctcga ggcggggtgc gagggcgagg tcccgctacg
     2881 gtggcccgcg gacagcgtcg cccaaggtgg gacgacgtgg ctgattgcgg ggcggtggct
     2941 cagtgaggga gatggggcgc ggggcgtcct cagagtgcgg tttgcccgcg tcctcgacgc
     3001 gacccctcgt ggccgcggcg gactccgcga cgcggtgtcg agacgaatac acaccctctt
     3061 cgggagccgg gcgccgctcg tcgatgcgct ggtgattgcc cggcgaaacg cgctcgaccc
     3121 ggaggtgcgc gagcgcttcg cgcaggcggg tctcgcccac ctcctgtgcg tagcgggatt
     3181 gcacgtaggc tttttggcgg ggtggctggc cgttctgctg cggcgtttgc cgctctcgcc
     3241 gacggcccgg tacctcgcgg gcgcggtggt cgtgctgggc tacgtctggc tcctggggtt
     3301 cccgccgccg gccacccgag ccggcgtcgg cctcgcggtg gccggctacg cgcgtttgcg
     3361 acaggagctg gtcccgccgg tggccgtgct cgcgctgacg gcgctgatcc tcctgctctt
     3421 cgatccgttc tcggcgcact cggtcggtgc gtggctttca ttcgcggcgg tcggcgcggt
     3481 cttctgggcc atccgggcga ccgccggagc gccctggtat gtccgattgg tggcaccggg
     3541 aatcgccgcc accctcatga cggcccccat taccgccctt gccttcggta ccgtggcccc
     3601 gatcggggtc ctggtgaacc tcctcgccat cccgctcgcc ggtgttgcga tccccggtgc
     3661 gatgctcgcg gtgatcgtga cccttctgtt gccgcccatc ggggccatgc tggcgcgcgg
     3721 ggccgggctg gggctcgcgt tgctcgatgc gttggcgggt gcgggagacc gggtgccgtt
     3781 cgggcacctc gtcatggtcg ccggaccggc ggctgcgttg acctggctgg tcgtgctggt
     3841 ggtcgcgtgg tggttgtggc gggcaccccg ccggcgttgg gtcatcgggg cccgcgtggc
     3901 gctcgtggcg gccatcgtcg cgtggaccgg ggcctttcgg gcgcaatcac tcgacgattg
     3961 tcggtgcctc gccatatctt tcctggacgt ggggcagggt gacgccgcgg cgctccggac
     4021 gccgcggggg cagtgggtcg tcatcgatgg cggcccgcgc gcgccgggga acgacgcggg
     4081 gcggcgggtc gtggtgccgt acttgcggcg gcatggcgcg cacgcgctcg cgctgatgat
     4141 ctccacccac tcggacgcgg atcacctggg cggactgccg gccgtggtcg ccgcgtttcc
     4201 accccggctc gtcatcgagc ccggtgagcc cctggcccgg ccgctgtact tcgaattcct
     4261 ggcggcggtg gaagaggcgg gctcacggtg gcggcccgcg cgggcgggtg accgaatcat
     4321 cctggacagc gtgatcatcc aggtcctgag tcccgacgat gactggatgg gcgtgcccgt
     4381 cgacgtcaac gatcacggcg tcgtggtgct cgtgacgtat ggaacgaccc gtgtgctgtt
     4441 tcaggccgac gcgggggtgc cggtggagga gcggctcgcg ggaacggtgg gacacgtgga
     4501 cctgctgaag gtggggcacc acggttcccg cagtgcgagc tcggaggatt ggctggcgga
     4561 actgtcaccg ccggtggccg tgatcagcgt tggcgcgcgg aaccgctacg gtcaccccgc
     4621 gcctgaagtg ctcgagcgcc tcgcgcgccg cggcatccgg gtgctccgga ccgaccagcg
     4681 cggtaccatc accttcacca gcgacggtca caatgcacca cgggacatcg gtcatcacga
     4741 ttgagcggtt catcgtggac caggagcgcc tgcaccccga ggcgaccggt gagctgtcca
     4801 atctgctcta cgatctcgcc ttggcgggga agatcatctc ccggcaagtc cgtcgcgcgg
     4861 ggctggtcga catcttcggg tccgccgggt cggtgaacgt gcagggtgag gtgcagcaga
     4921 agctggatat cttcgccaac gagaccgtcc ggcacagcgt gcagcatacc gggcggacgt
     4981 gcgtgatggc gtccgaagag gacgaattgc cggtacccgt gcctgaaggc aagcccctcg
     5041 gcaagtatgt gcttctgtac gaccccctcg acggctcgag caacatcgac gtgaacgtcc
     5101 cgatcgggac catcttctcg atccaccggc gccgcacccg tgcgggaact ccgggcacgc
     5161 tggatgactg cctccagccc gggtactcgc aggtggccgc cgggtacatc gtgtatgggt
     5221 cgagcaccat gctggtctac acgaccgggc agggtgtgca cggcttcacc ctggatccca
     5281 ctatcggcga attcctgctc tcccaccccg acatccgcag cccgggcgtg ggcaagtact
     5341 acagtgtgaa cgagagcaac tggaaccggt ggcccgatcc cgtgcaccgc gtcgtggcgg
     5401 ccttcaagac cggcgaagga cccatccagg cgcggaacgc ccgttacgta ggctcgctcg
     5461 tcgccgactt ccaccggaat cttctctcgg gtgggatctt cctgtatccg gccgacgcga
     5521 agagccccca ggggaaactg cggctgctct acgaggcgtg cccgctggcg atggtggtcg
     5581 agcaggcggg aggcgccgcg accgacggcc gccgtcgtat cctcgaggtg gagccgcgcg
     5641 agctgcatca gcgcacgccc ctcatcatcg ggtcgaaaga ggacgtcgag tatgcgacgc
     5701 acgtgctttc cactgaagga gccggagcgc gggcgtgacc tggaacggtg atccgcggcg
     5761 tgacctgggg gcgcccgggg cgtttccctt tactcgcggc attcacgcgt cgatgtaccg
     5821 cggccggcgc tggaccatgc gccagtacgc ggggttcggg acggccgagg aaacgaacga
     5881 gcggtttcgc cacctgttgg cggcgggcca gacgggcctg tcggtcgcgt tcgatcttcc
     5941 cacccagatg gggtacgact ccgaccatgc gatggcggag ggtgaggtgg gccgcgccgg
     6001 ggtggcgatc gatacggtcg acgacctgga gcggctcttt cacgagattc cgctcgacaa
     6061 agtgtcgacg tcgatgacca tcaatgcgac cgccgcgata ctcctcgcca tgtacgtggt
     6121 cgtcggtgaa gagcaggggg tgcgacgcga gcagctgcag ggcaccatcc agaacgacat
     6181 cctcaaggaa ttcatcgccc gcgggacgta catctatccg cccgagcctt ccctccgtct
     6241 catcgtggac atcctccggt ttacgagcga cgccgggatg aagttcaatc cgatctccat
     6301 ctccggctat cacatccgcg aggcgggcgc gacggcggca caggagatcg cgttcacctt
     6361 cgccaatggt ctggaatacg tccggcgcgc cgtggccgcg ggcctcgacg tgaacacgtt
     6421 cgcgccgcgg ctctccttct tcttcgccgg atacacggat ctcttcgagg aggtcgcgaa
     6481 gttccgcgcg gcccggcggc tctgggcacg gctgctccgc gagcgatatc aggcgtccga
     6541 tgacgcctgc cggctgcgct tccacacgca gacgggcggt gcgaccctga ccgcgcagca
     6601 accgctgaac aacgtcgtgc gcgtggcctt gcaggccatg gccgcggttc tgggcggcac
     6661 gcagtcgctg cacaccaact cgtacgacga agcgctggca ctgccgacca cccagagcgc
     6721 cacgctggca ctgcgcaccc agcaggtcct ggccaacgag acgggtgtgc cggatgtcgt
     6781 cgacccgctc ggcgggagct actatgtgga agcactcacc gaccggctcg agggtgaagc
     6841 gcgggccctg ctcgatcagg tggaagcgat ggggggctcc gcccgggcca tcgagcgcgg
     6901 attcttccag gacgcgatcg cccggagcgc gtatgccttt cagcgggccg tcgaggcggg
     6961 ggagcaggtg gtggtcgggg tgaacaagtt cgaagacgac ggcaccccgc cgtccattcc
     7021 cgcgcccgac tactcgacgc tcgcggccgg ccaacgggag cgcctcaaac gggcccggag
     7081 ccagcggaat gccgccggcg ccacgaaggc gattcaggcg gtgacggcgg gagcgaagga
     7141 cgcagggacg aacctgatgc ccgtgatcat cgacgcggtg cgcgcgcgtt gcaccgtcgg
     7201 cgagatcagc gatgcgctcc gcggcgtgtg gggcgtatat cagccaggat gacggcgggg
     7261 ggtccatgac tgaacggcgg cgccagactt ttcaagccaa cggggtggag gtcaccttcg
     7321 accccgacgt atgtcgccac accggcgttt gccttcgggg cttgccgctg gtattcgaca
     7381 tcaagcggaa acactggatc cggcccgaga acgcgagccc cgacgacgtg atcgcccagg
     7441 tggcgcgctg tccgagtggg gccctgcagg cccggcgtct ggaggagaag tgaacttgcg
     7501 tcgcgcgttc gagacggccc gatgggccgt ccgcgcctat ccgggatcca ccgcgctcct
     7561 cgccggcgcg tccgccgtca ccgtcggcgt cacgttaccc gccctcgcgc tcggtgccgg
     7621 acggctggcg ttgccacccg cgaccgatcc gggccttccg ctcgggttct cctccttcgc
     7681 ggggactccg gcaaccatgc aagcgcaggg catcgacgcg atcgccggcc tcctggttgc
     7741 gctgggaatc gcggcgctcg cggtcggcat cgtgacggcg ggatcgctgg cagtgggacg
     7801 cgcagccggg cgccgctccg aaatcagcgt gcggcgcgcg gtgggcgcgg cgcggtcgag
     7861 cctccgcctg agcggactcg tcgagggggt cctcctggcc ctgggggcca tcggcgtggg
     7921 cctggttccc gcggtcgcag ccgcccggtg ggcagtcgcc acgtggcccg gaacgctcct
     7981 gccggcgctg gtgggtgccc tcgcggcaat cgccgccgtt ggcggagtgc tccttctggg
     8041 cgcgctgttc ggtgtgcgcg cacctggcgg caccagggtc ccgtatccgg gcgcgtcgag
     8101 ccacggtctc gtgattcccg ccgcccagtt cgccatctca ctcgccatcc tgacgggggc
     8161 cgctcggact ggagcgcggg ccacgcagct cctccacagc ggggacccgg gtgtgagcgg
     8221 agcccgtgcg gcgtcggtga tagcgatcga gaacgaagcg agtgcgtccg ttcgggcgac
     8281 cggctacgag agcctcattc ggcggctcaa ggcggagccc ggattcggcg tggtgggcct
     8341 gtcgagcgcc ggtgcactca tgggtatggg ccccgtcgat ttcgtgacca ccgactgcgg
     8401 ccagtgccgc gagggattga tctacacgcc gttccgtccc gtaccggcaa tgatcatggc
     8461 gatgaccgcc gacaccttcc gcgccgc
//
LOCUS       DASUBP010000279        37077 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1222935, whole genome shotgun sequence.
ACCESSION   DASUBP010000279 DASUBP010000000
VERSION     DASUBP010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 37077)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37077)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37077
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1222935"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1253)
                     /gene="asnS"
                     /locus_tag="VFU75_10500"
     CDS             complement(<1..1253)
                     /gene="asnS"
                     /locus_tag="VFU75_10500"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEU5467312.1"
                     /translation="MPEQTRIEGLADRVGQTVTVRGWVMTTRSSGKIAFVTLRDGTGY
                     LQAVLSKKDVSEAAWEASGRLTQETSIAVTGVVRADARAPGGVELTVQDLAVLGASAD
                     FPITPKEHGTAFLFEHRHLWLRSRRQVAIARVRTEVIQAIRDFFYERGFTLVDTPILT
                     GSIGEHAGTLFSTEYFDLGKAYLAQTGQLYVEAAAAALGKVYCFGPTFRAEKSKTRRH
                     LTEFWMVEPEVAWNDSNDNMQLQEDFVCYIVKRCLERRKPELLELERDLAPLEAVRPP
                     FPRVSYTEAVEKLKALGADMEWGRDLGGDSETLLAKQYDRPVMVYNYPREVKAFYMKE
                     NPADPRTVLNNDCLAPEGYGEIIGGSQREDDYDKLLGRIRHEGLPEEAYGWYLDLRKY
                     GTFVHAGFGLGVERTVAWICGIPHIR"
     gene            1275..2369
                     /locus_tag="VFU75_10505"
     CDS             1275..2369
                     /locus_tag="VFU75_10505"
                     /inference="COORDINATES: protein motif:HMM:NF024753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HEU5467313.1"
                     /translation="MLCTVNAIACAPVIRHGPESQPVTWPVYLGTPAHDIDASEHLEG
                     APKSAWRANPGREIRGAAAAGKGVLAVGTTDRVVALLGTDSGQVLWDRRLAGTIRGGP
                     LLTGNRVYVATEETPSGRVYALEMRTGKTVWDEKTGGVAAAMALTKDGLFAGSETGVL
                     MALNPDTGGIRWRRRLSGSIQVAPVSTTAGLVVATTADSLYLVDPATGAILHRTPTAG
                     AVLGTPATDGARVYFATVTGTLAGVDAATLDTLWTRDVGSGVYGAPAIARDTLFVLTR
                     TGRLVRVPLGSPASAKSVELNLVSVAGPTPLADGVLVVDVGGVVRCVDADTGAERWRV
                     TVTGPVEQPPLVWEGRLILVAGRGEVVAYQ"
     gene            2366..2857
                     /locus_tag="VFU75_10510"
     CDS             2366..2857
                     /locus_tag="VFU75_10510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467314.1"
                     /translation="MKTTAVLMAFTLAARAGSAQTSLVDSTPRAAWKHAAVHYGKWAA
                     AAGAVAFTALAIQQHRHSNDAWNSLLAICNNNSQNCAVGPDGRYRNYQSEYLYQLAIY
                     YDHRARWRLIAGQVSLLGAVGLFVADLRGKSTTPPNQPVHPLKLAVTPTPDGATVALR
                     LSF"
     gene            complement(2875..4938)
                     /gene="recG"
                     /locus_tag="VFU75_10515"
     CDS             complement(2875..4938)
                     /gene="recG"
                     /locus_tag="VFU75_10515"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEU5467315.1"
                     /translation="MLLELNAPVKFLKGVGPKRAEALARLGVRTVGDLLYHVPHRYLD
                     ATTVTPFARARINEEVTGVGHVVSTGIIPTRRGLRVFRAVLKDDSGVLECAWPGQPFL
                     ERTIKAGQLLLVTGPVRYYHGKQLAPREFVILAEPGEAGPDQGVVLPVYPATEGLTHK
                     QIRALIHPHLDALLELVRDPHPPALRAKVGIPELRLALAAVHRPGTPADAENGRRRLA
                     FDEFFDQQLVQARARFLAKRARTGIAFQLRRELTTALKEHLPFELTGDQRHAIREITE
                     DMTAPQRMHRLLMGDVGTGKTVVALFAMLLAVENEFQAAIMAPTELLAEQHHATLTRL
                     LAPLDLRPDLLIGRLTGPEKDAARDRIARGASRVIVGTHALIQESVSFRRLGVAVVDE
                     QHRFGVEQRAALVEKGDAPDVLLLTATPIPRSLALTLYGDLDVTRLEERPPGRGTIKT
                     ALRTDASRAKIYAFLQSEIDAGRQAYVVYPVIEESEKADLRAATTMADTLKKTFGESR
                     VGLVHGRMKPEERDRVMRAFRDNHVPLLVATTVIEVGIDVPNATVMVIEHADRFGLAQ
                     LHQLRGRVGRGAAASHCILISDVPEAAGRLKAFTETTDGFAIAELDLKERGMGELAGA
                     RQSGGQALRYVHWDTDLPLLEAARVAAGAIIASDPALTRPEHAAYRARILQRYERGFE
                     LFRVG"
     gene            complement(4938..5822)
                     /locus_tag="VFU75_10520"
     CDS             complement(4938..5822)
                     /locus_tag="VFU75_10520"
                     /inference="COORDINATES: protein motif:HMM:NF012662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEU5467316.1"
                     /translation="MAAPDGVALMGLWGKIGRVLGRRDQAARADAERILLEADFGPAL
                     AEELLGRLEKVPDRELEAALEREVRALSGNGVPAELLRAGTAPTVILLYGVNGVGKTT
                     TAAKLARRLQGQGRTPLLAAADTFRAGAVSQLRIWAERLGVPFVGPSADGTGDSAAVA
                     FDAVRAAAARGVDTVIIDTAGRLHTDDRLIEELKKVVRAVGKAHPAHDAPHESLLVLD
                     GSVGQTAVRQAEAFAKDIPLTGTIITKLDGTARGGAVVALARAVPAAVPRFVGTGEAV
                     DDLSLFDPDAFARRLVRD"
     gene            complement(5795..7540)
                     /locus_tag="VFU75_10525"
     CDS             complement(5795..7540)
                     /locus_tag="VFU75_10525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467317.1"
                     /translation="MRGLNTWGPVAFRVTGVGCLVLLLWNPAITRRAPGGTAPVVLLD
                     ASLSMSAQPGLWAEALDSARALARGGVILRFGETVAAFDTAVPHEGASSLAPALTAAA
                     ARGGPIVIVTDGAIDDRDALPSDLITRARVVVVGHGRPPDAFVASVEGPNQVAPGDTV
                     RLRVTYGRSGKRESGIGNREATLNVRLSDRILTSRKVVLPDSGTVNVDLTFPVSRFPT
                     GWSALSVGVQWAGDSEPRDDSRSFPIEVTSEPAAVVLGAPPDWDATFLARTLGDVARV
                     PVKYFTQITAGKWNEGVTLAPVSVERVRRALRGARLVVEVGDPSHWPKVSAPALLTWN
                     SAGGSAGDWYLTPSSSSPLVGALSTVPWDSLPPVLSALPSLPDSGAVPVLEATRSRRG
                     QARPVVFVAQSSRGRRAVVNLAGLYRWDFRGGASQQAYRAAVAGLVDWLLAGGSTATD
                     WARPDSLDVPDGLPVTWRWSGPGSPKPLGVAFASASGTTTDTLRFGADGHAQVRLPPG
                     TYRYALAAGHGHGLIVVDRYSEEWRRGSALEDQPGRAAADRVVLDWRERWELFALAIL
                     VFAAEWTWRRRMGLP"
     gene            complement(7528..8226)
                     /locus_tag="VFU75_10530"
     CDS             complement(7528..8226)
                     /locus_tag="VFU75_10530"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HEU5467318.1"
                     /translation="MDLYPAVDVAGGRVARAPAVPAGADDPLAVARAFVAAGAQWLHY
                     VDMDRAYRRGENHAVTRQLLHAGLGWVQVGGGLGGEKDVREVLTWGATRVIVGAAAAL
                     DAGTIDRLLSAFGASTLGVALDVADGRLAPRGAAKVDPGTSPLGLARRLQKQGIRTVV
                     YTDVPRDGALAGADLDAAAGVAELGLEVIVSGGIAGLDELTRAKRLGLAGIIIGRALY
                     EGRFTLTEALQCVA"
     gene            complement(8241..9503)
                     /locus_tag="VFU75_10535"
     CDS             complement(8241..9503)
                     /locus_tag="VFU75_10535"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HEU5467319.1"
                     /translation="MTRPQMVRSALAGAGVVVLVGGGLIARAWMHKPLAAPPVVVTAA
                     YQEYSDTLRRDETLGDLLQRAGITGRAYGALLSAAHVLDARRLRPGLVFAFRRPIADS
                     IADRVSVRTAPEQRIWLTRTGVLWNEQVESIAWTSTRVRVTGAITDNLYDGLDAAIPD
                     SVLPRGQRVALAWAIADVYDWEVDFTRDVRAGDRVDILFERLESPTGERRFGRILAAR
                     VDAAKTPNYAFRFAGVDSARAGYYDETGRSLRRAFLRAPLQFRRISSGFGNRYHPILH
                     RWRKHEGIDFSAAYGTPVRATADGVVYRLVYEADGYGRMIELRHANGIKTIYGHLSAY
                     AKGLHVGQRVTQSETIGFVGSTGLSTGPHLHYEFLVNGQATNPNRKDMGAGTPVPKSL
                     QASFNAVRTTLLSLLEPAPVASLASAGD"
     gene            complement(9503..10684)
                     /gene="ftsZ"
                     /locus_tag="VFU75_10540"
     CDS             complement(9503..10684)
                     /gene="ftsZ"
                     /locus_tag="VFU75_10540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEU5467320.1"
                     /translation="MIFELEETVAQNARMKVVGVGGGGGNALNRMVDEGVQAVEFISV
                     NTDAQALLKNKSDIKVQIGKKLTRGLGAGARPEIGRQAIEENRDEILHALQGADLVFV
                     TCGMGGGTGTGAAPIISQVAKDLGALTIGIVTKPFLFEGRKRMKQAEMGIAEMRKHVD
                     TMIVVPNERLLAVVGKGIPFQDALKKADEVLLHATQGISSLISVTGIVNVDFADVRTV
                     MQNGGSALMGTGVGRGENRALEAAQQAISSPLLDNIQISGATGVLINITGGEDLTLGE
                     VTVISEAIHEAAGQEAEIIFGAVNDPAMQGEVRVTVIATGFDKAGVAGEAAAQAATAG
                     RPGPGVLPFRPATRPTPPPPPGYAKPTEVQPKRPSAPPPATTAEVNEMEIPTFIRRQM
                     D"
     gene            complement(10704..11990)
                     /gene="ftsA"
                     /locus_tag="VFU75_10545"
     CDS             complement(10704..11990)
                     /gene="ftsA"
                     /locus_tag="VFU75_10545"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEU5467321.1"
                     /translation="MSKNERRLVAGLDLGSTKTCAVIAEIAGDLPRVPVVKILGVGVA
                     KNTGVRRGMVRDIEETARSVTAAMRDASRMAGASVTEVTCGIAGEHVSARTSSGVVAI
                     RGEEIAGPDVERANEVARAVALGRDLQLLHGIPQEYKVDQQGEISDPVGMTGTRLEVE
                     MYLVTVQATAAQNLRRSVERAGFKVADIVLEPLAASHAVLTDDERELGVALAEIGGQS
                     TGVAIFQKGKIRHLASLKFAGGFVTSDLVQGLSLTQADAERVKERFGSAYQPLVNPDE
                     MVDLPSTPGQGARQAPRDMVAHIIHQRMDEILQLVRGELTKAGYDDPKLLPAGVVLTG
                     GTAHLPGIVELGREVFAMPVRAGIPERGLGGLIDSVQAPRYAVPVGLAMYAARRVVQG
                     AMSGGQGGGGGAGSLVTGPGMDKLLGPVKRWLQDFF"
     gene            complement(11987..12700)
                     /locus_tag="VFU75_10550"
     CDS             complement(11987..12700)
                     /locus_tag="VFU75_10550"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HEU5467322.1"
                     /translation="MSVILEALKDRRVWIPLVVLGALGGLWVGGPAVGRHLEFFRLRQ
                     VELVGVRNLSPEAVIQALGLSREASLFDPTAPLEARVRALPGVADARIKRHLPGALVV
                     IVREVDPAAFVPGPRGLVVEDAAGHPLPFDPARTVLDLPVAASADSGLASVLGLVRAV
                     DPVLFQSITSARRISRSAVALDEGGAHRRLLLGADPQPEVIQAVVLVQQDLAAKGRTY
                     AELDARFAGQIVVRRKASS"
     gene            complement(12697..14088)
                     /gene="murC"
                     /locus_tag="VFU75_10555"
     CDS             complement(12697..14088)
                     /gene="murC"
                     /locus_tag="VFU75_10555"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEU5467323.1"
                     /translation="MTLFAPGDPRPVHFMGIAGAGMSALALIAKRRGVQITGCDQDPA
                     GAQDLVALGVPVSRGHDPSHLEGARAMVVTAAIPPHHPEVERARILGIPVIRRADALG
                     EAVAGGTVVAIAGTHGKTTTTVMVTEGLAAAGRNPTGLVGGRVAGWGGNARQGSSELF
                     VVEADEYDKAFLSLQPTVAVVNNVEPDHLECYGSIEALEGAFAEFAGRAQRVIASADD
                     PGARRVVHGLKVPVWTVGTDSGDVRIQDAHFSPTGSTARVKLPNGDAVALTLQVPGAH
                     NLRNAGVALAAVHAVGANVRSAAAALAEFRGVARRFERLGEVDGVMVVDDYAHHPTEV
                     AATLAGARQAFPDRRVVAVFQPHLYSRTAAHGKALGQALAAADVAVVAPIYGAREAPM
                     AGVTSDLVIDAARAAGADVVKVADRAKLTADVAALLQPGDLLLTMGAGDVTRVGPEIL
                     TKSRSAGRERAPR"
     gene            complement(14131..15216)
                     /locus_tag="VFU75_10560"
     CDS             complement(14131..15216)
                     /locus_tag="VFU75_10560"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEU5467324.1"
                     /translation="MKILISGGGTGGHLTPALALADTFRQMRPDLEIHLVGAERGIES
                     QILPQHPYPFTLLPLQPIYRRQWWKNLQWPVLAVRLWRRIGALLDEVQPTLVVGTGGY
                     ASGPVVYHAQRSDIPTAIQESDARPGIATRWLSGRARQIHLGFPEAQARLRPGDRTEV
                     FTFGNPITPPQPGDRAEAVKALGLDPALPTLLVFGGGQGARAINYAVAGVLEHGHLEG
                     MNLIWGTGVANYPGLSGYARSGKVVVQGFLDPMAPIYRATDLVVCRAGALTIAELCAW
                     GKPSLLIPLPTAAANHQLENARAMAEANASVLMEESSLTPHSLATAITQLISDSARRQ
                     SMARAALGRGRPTASRDVVSRLLTLVS"
     gene            complement(15213..16373)
                     /locus_tag="VFU75_10565"
     CDS             complement(15213..16373)
                     /locus_tag="VFU75_10565"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HEU5467325.1"
                     /translation="MKENAWETRLLGILAAILIVVGLVSVYGASSLVNTQGGVVGASY
                     FFKQAIGAAIGGLGATILARTDYHVWQRWAWPLLGLTLLLLIIPVLPFTRAIAPILNG
                     ARRWVNLGFTVVQPSELAKFTLVCWTAMLAAKKGDQIRTFKFGLLPFFVVLLPVIGLV
                     FLEPNLSMALLLTMLAGTVLFAAGARIGHFLLIGIAAAPVLFGAVASAQYRLARVSHF
                     FSSGTAPSEAQSQVHQSLVGIGAGRLIGVGFGQGQQKLGYLPYAYSDFIFSTIAEEWG
                     FIGVTVIILCFAGICWLGFRIARRAPDPFGQFLATGLTALIGVTAVLHIAVSLAVMPA
                     TGITLPLISYGRTSLFVSLAAIGVLVSIGHGQQSQRKTAGRRRATSPAGASK"
     gene            complement(16370..17722)
                     /gene="murD"
                     /locus_tag="VFU75_10570"
     CDS             complement(16370..17722)
                     /gene="murD"
                     /locus_tag="VFU75_10570"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEU5467326.1"
                     /translation="MKLPESWRHGTVAVIGLGKSGVAATRLLAREHVKVYASDGSQHP
                     YAEDAVRALGTLPGVEVELGHHNLARIQHAAGVVVSPGVPPDAPPLKTARESGIPIVS
                     ELDVGCRALEDTRVIAITGTNGKTTTTALIAHLLRSAGLPAEAVGNIGRPVSDMALSS
                     PHPQFLAIEVSSFQLHDSPHFAPDIGVLTNLAPDHLDRYASVDEYYADKAMLFRNAHG
                     KNAWVVNGDDRAVLDMVKSVTGTKLQFSLEWKADAWYDAAAGALMLKNGVLLHREALN
                     LLGAHNVANALAAALAVHRAGVAPSAIAEGLKSFRALPHRLEPVREVDGVQWINDSKA
                     TNIASTVVAVEAMTRPYVLLLGGRHKGEPYTRLGPLLKPQCRLVIAYGEAGPIVAKDL
                     EGQVPIERGTTFADVLARAQKAARPGDAVLLSPACSSYDQFKNYEERGATFRQFAEGL
                     "
     gene            complement(17719..18837)
                     /gene="mraY"
                     /locus_tag="VFU75_10575"
     CDS             complement(17719..18837)
                     /gene="mraY"
                     /locus_tag="VFU75_10575"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEU5467327.1"
                     /translation="MFYHLFAPLGKKYILFNLFNYITFRIAGATATALIVAFLVGPWI
                     IDILRRRNVGQVVRAEGPASHQGKRGTPTMGGLIIIIATVIPTLLWARLDTRYVLVAV
                     VATLWMGAIGFLDDYLKIVRGKSQGLVARWKLVGQVTFGIALGTFLVLWPFVLEWRTA
                     TQVPLFKYWFLVLPWPIYIAFVTLVVTGSSNAVNLTDGLDGLATGLTAIVAGSFTIFA
                     YVFGRADYSKYLGVLYSPGAGELAVFCAALLGASLGFLWFNAHPAKVFMGDTGSLAIG
                     GALGAIAIMLKAEFLLLIAGAVFVAETLSVMIQVNVYRWNRKRRGKEYADAHRVFRMA
                     PLHHHFEKMGWAEPLVVMRFYILGVLCAAIALATLKVR"
     gene            complement(18854..20227)
                     /gene="murF"
                     /locus_tag="VFU75_10580"
     CDS             complement(18854..20227)
                     /gene="murF"
                     /locus_tag="VFU75_10580"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEU5467328.1"
                     /translation="MKSETPTPAAPPEPWTAVQVSDALGVKVTASGPFSGVSTDTRSL
                     TPGALFLALAGEHFDAHAFLDQARAGGAAAAVVRRGTPNIEGLPFIEVPDTLVALGAL
                     ARARRRRLADGSPVVAVTGSSGKTSTKELIRAALGVRYHVHATAGNLNNLVGVPLSIL
                     AAPAGTNAMVIEAGANLPGEIPRLRDIIEPTIAVVTNVGYAHVEGFGSLEGVMREKVS
                     LVEKAEVAVVGTEPPGLAVAARAKTRTVVAGAGAGTDMHPDAADLDESGHPRIRWGGR
                     EVTVPVTGFHQIDNAAIAVAVAREAGIRPGDALQGLTAARVPGGRGAVTEVRGLTVID
                     DSYNANPGSLRRAVEFARWLADKRSRPLAVVVGTMLELGAESDKLHRAAAAEIVAAAP
                     ALVGAVGAFEAAFSPFAAALKDRLVVAPDAERLGPLLKQRLTGNEVVLLKASRGVALE
                     RVLRHLN"
     gene            complement(20224..21672)
                     /locus_tag="VFU75_10585"
     CDS             complement(20224..21672)
                     /locus_tag="VFU75_10585"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEU5467329.1"
                     /translation="MIELTDIVAALRERGLLVHAPVVLPAITGVTADTRKLEKGMLYC
                     VVRGAIQDGHTYVSKATEQGAAAALVETAQPGLLPQVVVKDGRRAAAVAAEVWFGYPA
                     RKLNLVGVTGTNGKTTSVTLGRHVLSLLWPTGSIGTLGAFAPDGTPVESEAGNLTTPG
                     PIDLQETLALLVERGARGAAMEVSSHSLDQGRVDGLVYRAAIFTNLTRDHLDYHKTFE
                     HYFQAKAKLSTYLAPDGIQVVNAEDPAWQNLPGGHRRITFGETKGDVRAERVKTDGTG
                     SRFTLVSPQGHADVNLPLLGAFNVANALGVAACALGLGAPIDGVAERLTHAPQVPGRM
                     ERIASRPCVVLRDYAHTPDALERALETVRPLTAGKVIAVFGAGGDRDRGKRAPMGDIA
                     SRLADVVIATSDNPRTEDPNRILDDVEQGMQGRPHHREADRRKAIALALSLAHAKDTV
                     ILAGKGHETYQVIGHEKQPFDERQVVRELGAS"
     gene            complement(21669..23600)
                     /locus_tag="VFU75_10590"
     CDS             complement(21669..23600)
                     /locus_tag="VFU75_10590"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEU5467330.1"
                     /translation="MAKLTTRVSFIEGSLGLLLLALLVRSAQVQLFQGARWSAEAKQA
                     RTEHVVLEAPRGALLDRHGTPIALTQETYHVGIAPNELRDPDHDPETIARVLHLPLMR
                     VEDELSRHYAYFAGPFTAGTVQGLRDVRGVHLEPVLNRFYPSPDFARAFIGRAGEGGQ
                     KPSGLERTLDTLLAGTPGAAVVLKDRAGREYDSPARRLQSPVPGNDVVLTLDAELQEI
                     AQRSLRLALEKMDADGGDVVMLDPATGEVLAVASERRDGVSRPSAFIDAYEPGSVAKI
                     FTAAALIADHRISLTDRVSGENGKYKMQDRTLVDDHPLPMLNLADAIRVSSNIAMAKF
                     VTRLDHDDEYGMLRDFGLGTPTGIEFPAEASGRLALPKEWSRPTPTSLAIGYEVSVTP
                     LQIAAAYGAIANDGVLLEPTLIREVRAPNGDILYRHQPEPVRRVVSPAVARELRQLLA
                     GVVSSGGTGASAALATFTLAAKTGTSRQVVNGHYKAGAYNASFAALFPADHPQLVVVV
                     KVENPRKGSYFAAETAAPVTRAMLEQALASRTVALDRARLSAVAPAAGVGELEEDPGL
                     APYVVSWPPAHDTAAPAPPRPVPDVSGRTLRDAVRLLHRRGFHVVIKGWGSVQKTWPA
                     AGAEAAAGSLVTVFGDTPQ"
     gene            complement(23602..23889)
                     /locus_tag="VFU75_10595"
     CDS             complement(23602..23889)
                     /locus_tag="VFU75_10595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467331.1"
                     /translation="MVLKGRHWVMIWLLAFLVAAGSVVARQTAALRIARALNQARDSL
                     SSLQSRRAEYERRIRQAASRSVLVPRAQALGLRLPADSEIVIQPVPDTSAP"
     gene            complement(23890..24789)
                     /gene="rsmH"
                     /locus_tag="VFU75_10600"
     CDS             complement(23890..24789)
                     /gene="rsmH"
                     /locus_tag="VFU75_10600"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:NF013919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEU5467332.1"
                     /translation="MSFHDPVLADVIRSWAAGSARAVDATAGGGGHTVILRGSGALVL
                     AIDRDPEALAEAQARIVDDGVTWLAAPFGAPKTLDAVRAFQPDRILLDLGVSSHQLDA
                     QARGFTFRPGAALDMRMTPDVGVSAADILNTWGEERLAKAFAEDADEPKARRLARSIT
                     RRRERGPFMTSDDFVNAIREALGSRSGPPDFARLFQALRMAVNGEKEQLTTALPAFRD
                     ALTPGGRLGVIAYHSGEDRIVKHAFREWARECICPPGQPVCTCRGHALGTLRTRKAIT
                     PGPAEMAANPRSRSAHFRDFEVA"
     gene            complement(24868..24941)
                     /locus_tag="VFU75_10605"
     tRNA            complement(24868..24941)
                     /locus_tag="VFU75_10605"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(24905..24907),aa:Asp,seq:gtc)
     gene            complement(24991..26790)
                     /locus_tag="VFU75_10610"
     CDS             complement(24991..26790)
                     /locus_tag="VFU75_10610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467333.1"
                     /translation="MRVFRLVAVMVMGAASVASAQLAITQPTEKLLLLPLPGAAAGDS
                     AASIAVMDLARDRVQQLARYKVQNIPKSKICEAYQSFGFACDPAPSGAMYIVDQSQAG
                     QFAKSVGVNSYNVGTFSHTGGLYIAHVRVISGGSGFASAFTVSGASPPAVAESLGQRL
                     VTVIKAAEFARECDDRRSHGNLNGALESAKKAFAIDPNLTAAHLCVLTVYEAQRQPPD
                     SIIAAGYRALKGDSMNTTAWSRIAGSYQQKGDTLKWLDALQSWAAVEPDNIPLQLSVA
                     DQLMKNHAYPKATALLKTVTAHAPTNAAAKDMLRRSCIEGSQWRCVLDIGAAEEKDSA
                     AKLNDSTELKIMIGAANALPDTQAWVHYTNIAFKDFPKNPGFCNIRGQALEAIGQVDS
                     AIADYKACAALTPNDARPALLVAATIINHNVYDSTKAVGDSAHKLAYRTAYADRFDQA
                     KPYLQPAITGTDSVLKINAAALMLQAASGIARSAQLWDRAYPWSMDLLALIPQAGPAD
                     TAGAKQQIRIQGNFWYGVSSVLSLGGPYKDMTDHKSCSEAKEINERIAKTRDALFLGG
                     KVSPSFAQQMLGILDKYAKAMPQVKAAFKCTNF"
     gene            complement(26831..26905)
                     /locus_tag="VFU75_10615"
     tRNA            complement(26831..26905)
                     /locus_tag="VFU75_10615"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26868..26870),aa:Val,seq:tac)
     gene            complement(26918..27100)
                     /locus_tag="VFU75_10620"
     CDS             complement(26918..27100)
                     /locus_tag="VFU75_10620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467334.1"
                     /translation="MPDTQSRERLHFGPRNYAAFGAALVCLAAGYVSLNAGSTTLAPV
                     LLVLGYLVFIPVGLAL"
     gene            complement(27137..27958)
                     /locus_tag="VFU75_10625"
     CDS             complement(27137..27958)
                     /locus_tag="VFU75_10625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467335.1"
                     /translation="MTVVGNVTVVWWPPQGNLAAALADEADRPNPFPGIDPQPDRPLR
                     IFLAPNRAIYDSLTRGRLPSWSEGAAFPGSGAIVLWSVGSPSDLRVSLRHELAHVALR
                     RRVRHSLPLWFEEGYAAVAAGEWNRTQAFELDWHLLGGPALELDSVDRALRGRAGEAA
                     GAYALATTAVGMLQRWGGPAGLHPFIDALATQPTFDDAMRSTYHVTLDHFEELWAQDV
                     HRRYGWLAGLGAATLFWGVIGLLLVGLVWRRRKRDAPRRAALDEGWVIPPDEPNP"
     gene            complement(28009..28872)
                     /locus_tag="VFU75_10630"
     CDS             complement(28009..28872)
                     /locus_tag="VFU75_10630"
                     /inference="COORDINATES: protein motif:HMM:NF024824.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5467336.1"
                     /translation="MADSPVVLLRAQYDAIARKLDTAKATAEQEAVKREIIAFFKHVD
                     GLLGDLGQLKEDIRQLVEKYKQVSASAAEASAPEFTGTRPAVHADHIGASTFIEKGWS
                     LISLGDHAGAIQALQKALALSPGETQAESMLGWAQMLHEDYDDALATFQKVLMKEPAN
                     SLARINVGYICLKKKIFGEAIEHLSKAIRLDNDKKATLYAHFYLGLVYLQREMYEDAV
                     TFFQKTLKLGPNLIEAYYEVGRAFWFAGDQAQAKQWWQDGFKANKFNPWGKRCQEMLE
                     AVGRGEEPPRP"
     gene            complement(28872..29348)
                     /locus_tag="VFU75_10635"
     CDS             complement(28872..29348)
                     /locus_tag="VFU75_10635"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEU5467337.1"
                     /translation="MSAGDDVQLVTFRVAGQDFAFHIFQVERILRYERPAPLPKAPEF
                     LEGVLRYEGAAVPVIDLRKRLGVPAPVRDDTRTVILEYDGGKIGLVVDVVTEVLQVGA
                     EEVTPPPRIVKGLAAAYVTGLVVTGGRTIIVLNTAQLLASKEKVALEAALTEKASA"
     gene            complement(29345..29761)
                     /locus_tag="VFU75_10640"
     CDS             complement(29345..29761)
                     /locus_tag="VFU75_10640"
                     /inference="COORDINATES: protein motif:HMM:NF013731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HEU5467338.1"
                     /translation="MKGRGVGYVMVRVGGRPYGLPLGPVLEVGDLGEVLDVPRALPAV
                     RGLTPVRGRLVPLIHLGALLKNQTPPVERGGAMVLVTIGGRLVEFEVDDADEVVREAP
                     LPVPPGESLPWASGMGRRDGGLVPILDLEALGERIQ"
     gene            complement(29758..30849)
                     /gene="cheB"
                     /locus_tag="VFU75_10645"
     CDS             complement(29758..30849)
                     /gene="cheB"
                     /locus_tag="VFU75_10645"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: protein motif:HMM:NF001965.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-specific protein-glutamate
                     methyltransferase CheB"
                     /protein_id="HEU5467339.1"
                     /translation="MSPPSDTRPTVLVVDDSALMRRVLVDVLGGGSRDNGPFRVVGTA
                     RDGFDAVRKVHQFQPDVVTMDLEMPELDGLGAIGYVMSESPRPIVVVSAHAGPGTAAA
                     IRALELGAVEIVAKPDERNRESLEALGPLLVDAVRRALAADLSRVTVLARPPKPVAHA
                     PEMGLKGRAKLAVAIAASTGGPRALTELIPHLHPGRMACVLVVQHMPARFTRSLAERL
                     DSMSAIRVVEADEGAPLVADTAYVAPGDYHMRVEPGPDGPIIALDQSAPIWGVRPAAD
                     PLFRSVAKTFGNKALGVVLTGMGRDGADGLRAVHDAGGMGIAQSKETCVIGGMPGAAV
                     QAGGVDAVLPLPEIADRVSGELIRRAARA"
     gene            complement(30833..31294)
                     /locus_tag="VFU75_10650"
     CDS             complement(30833..31294)
                     /locus_tag="VFU75_10650"
                     /inference="COORDINATES: protein motif:HMM:NF015908.4"
                     /note="catalyzes the conversion of glutamine residues to
                     glutamate on methyl-accepting chemotaxis receptors;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HEU5467340.1"
                     /translation="MADWAADQGASDLLTLGLGSCVAIMLYDKETKIGGMAHVLLPSR
                     SLARDTTNPAKFPETAVPLLVQRMTQLGADPRRLVAKLAGGASMFGALMTPGTIQMGE
                     RNVVASRNALRDAAIPIVAEAVGGGQGRSVRFHVSDGSVEIRTVGADVTTL"
     gene            complement(31333..32151)
                     /locus_tag="VFU75_10655"
     CDS             complement(31333..32151)
                     /locus_tag="VFU75_10655"
                     /inference="COORDINATES: protein motif:HMM:NF013867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HEU5467341.1"
                     /translation="MTPDEDERAFRALTQQIATARGFSCEAYKDKCLKRRIAVRMRAC
                     GVHTYDAYATRLIQDAKEYEHLLDALTINVTKFYRNAETWDAVRPMVAAAWKEREGQY
                     AAWSAGCASGEEPYTIAALLADIARVAGMEAQLGRTRVDASDIDRASLERTAAAQYPE
                     SAFVEMPKAMADRYFTKPAPRQPVPLIKNLVHAARNDLLKERAPRPPYDLIFCRNVVI
                     YFDRPSQEKLFVRFAESLRPGGLLVLGKVETLFGDSRQLLELEDPRERIYRRPL"
     gene            complement(32148..33776)
                     /locus_tag="VFU75_10660"
     CDS             complement(32148..33776)
                     /locus_tag="VFU75_10660"
                     /inference="COORDINATES: protein motif:HMM:NF025690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HEU5467342.1"
                     /translation="MAIKGSLKEASLPDVLQLLALGQKTGCLSIADRQNFGYIFFEKG
                     RICYASIVNRRDRLGDILVKHEKITQQQLEDGVHLQGQSKNRDKKLGAILVEQGAITQ
                     GDLERYMRVQIEESVYFLFTWTQGTFNFEADVRPERQDFLVAINPESLLLEGARRVDE
                     WSLIEKKIPSFDVIFIVDRDHLEISEVSLTPTQERLLPLIDGERDIHTLIEDTGLGEF
                     EVGKALYGLLTAGFLHRAGRTTATQTEDAKVTDARVEEHRNLGVAFYKTGMLEEAARE
                     FRRVTELRGEDANALFFTGLVALRQARWREAMEAFRLAAEKGGSRPAVLHNLGLAYEH
                     LGRLEDAESAYAEAATRNRNDARIALGWGVVALKRGDYESAAGRLDRAQELFGEKVPG
                     VWYWARTLAAAASGEFEQADELAEAAVAAFPENAVLRNNRAVLRELAGDLPGAEELVR
                     SALRDEPSHPQLSKNLGDLEYRAAHYDEAWEAYQRTIELSPEIGDDVYFKLGNIAYKR
                     NDRELASQMWKKALELNPAHELVKSNLDTLSALK"
     gene            complement(33777..35714)
                     /locus_tag="VFU75_10665"
     CDS             complement(33777..35714)
                     /locus_tag="VFU75_10665"
                     /inference="COORDINATES: protein motif:HMM:NF012529.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HEU5467343.1"
                     /translation="MTSALNPKFSFDSYVVGAANRLAVTAGRTVAENPGSAYNPLFIY
                     SGSGLGKTHLLMAIGHGAKKVAPNLSVEYLTLDEYVEAFHAAIAAGQGDTFRRRFQAV
                     DMLLVDDVQFLTNRKEMQAELLRLTEGLQAAGHQVVLASDRPPGEIADLDERLISRFS
                     GGLVVDMGIPDYETRVAILRKKAEERGTGFQSGVLETVAGVEFANVRELMGALNRLIA
                     FQAVNDTPITEAAARQVLGIGSAEDAAAAQPAAASAPAPDEFSNFLSDVTATVGRAVE
                     AWRARVSEAILRWEGEGFRTKRLEALLAQETPGAVDQVISGFVKDVERLKVLAAEVQM
                     LEPQAAGDSVFRDPERLDEAEKVAVRVREGAAPPPAPSAAFPFDTFVAGPSNEKELAA
                     IHDVVQSPGKKFNPLVLVGGSGLGKTHLLNAIGLELATKARAVVACLSTQTFIDEVIA
                     AIEGDRVDWWRARYRRATALLLDDVHLLAGKDRTQEELFNLFNVLQDKDRQLVFTAPA
                     APNTLEGLEERIQSRLAGGLVVTLAQPDRVVRRAVLERSLGKVEGSLLDYLADRPATS
                     IRAMMAEVQRVKDAADAQSQPMTAGLARAVLGGEVAPRRRTSGFRTSGIVVSSAGGVR
                     SREKMVWDWPVPADRMIEDAR"
     gene            complement(35711..36310)
                     /locus_tag="VFU75_10670"
     CDS             complement(35711..36310)
                     /locus_tag="VFU75_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015347803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase domain-containing protein"
                     /protein_id="HEU5467344.1"
                     /translation="MSLVNFTTREITCKIVYYGPGRSGKTSNLHYIYGRVPEARRGRM
                     VSLATQTDRTLFFDFLPIDLGSISGFTTRFQLYTVPGQVYYNATRRLVLQGADGVVFV
                     ADSQGRQLDENLESLQNLHANLLDQGVDVRAMPLVLQYNKQDLPKELILTPAEMDDAL
                     NFRAVPSFSADALHGTGVFESLKGISELVLKKLAQGAPA"
     gene            complement(36314..36784)
                     /locus_tag="VFU75_10675"
     CDS             complement(36314..36784)
                     /locus_tag="VFU75_10675"
                     /inference="COORDINATES: protein motif:HMM:NF015232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HEU5467345.1"
                     /translation="MTARAVEAWTLEPLTTFVQEAGVRLVLLMTPAGQVLAQSGFARA
                     VDVMSAAALGAAIFASTTEMARQLGEAPFAGLSHQGEKHGIFLSSVSTDRGPVVVLAV
                     YDLSATSLGLVQLFFDQLATDLRGASPKGSEQKQVLAADFERELTNSLNSLFGR"
     gene            complement(36781..>37077)
                     /locus_tag="VFU75_10680"
     CDS             complement(36781..>37077)
                     /locus_tag="VFU75_10680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467346.1"
                     /translation="RGAMVVAGADGLVVAESLMEGIKGAAVAALAASLATRLGRAMEA
                     GGVGSDVFWHLQAERGALLVVPARSGMLIVAVAEPGVNAGLVRLELLKAAEGVA"
BASE COUNT         5883 a        13133 c        12294 g         5767 t
ORIGIN      
        1 cggatgtgcg ggatgccgca gatccacgcg acggtccgct ccacgccgag gccgaatccg
       61 gcgtgcacga aggtcccgta cttccgcaga tccagatacc agccatacgc ctcttcgggc
      121 agcccttcgt ggcggatgcg gcccaagagc ttgtcgtaat cgtcctcgcg ctggctgccg
      181 ccgatgattt ccccataccc ctcgggcgcc agacagtcgt tgttcaggac ggtccgcggg
      241 tccgccgggt tctccttcat atagaaggcc ttcacctccc gcggatagtt gtaaaccatc
      301 accggacggt cgtactgctt ggccaggagc gtttcggagt caccgcccag gtcccggccc
      361 cactccatgt cggcgccgag cgccttgagc ttttctaccg cctcggtgta ggaaacccgg
      421 gggaacggcg ggcgcacggc ttcgagtggc gcgagatccc gctccagctc cagcagctcg
      481 ggtttccggc gctcgagaca gcgcttgacg atgtagcaga cgaagtcctc ctggagttgc
      541 atattgtcgt tcgagtcgtt ccaggcgact tcgggctcca ccatccagaa ttcggtcaga
      601 tgccggcggg tcttggattt ttcggcgcgg aaggtgggac caaagcaata caccttgccc
      661 agcgcggcag cggcggcttc gacgtagagc tgcccggtct gcgccaggta cgccttgccg
      721 agatcgaagt attcggtgga aaagagcgtt ccggcgtgct ccccgatcga tccggtgagg
      781 atcggcgtat cgaccagcgt gaagccgcgc tcgtagaaga agtcgcggat ggcctggatc
      841 acttcggtgc ggacgcgcgc gatggcgacc tgccgccgcg agcgcagcca gagatggcgg
      901 tgctcgaaga gaaacgcggt gccgtgctcc ttgggcgtga tcgggaagtc ggccgacgca
      961 cccaggaccg cgagatcctg taccgtcagc tccacgcccc ccggggcacg cgcatcggcg
     1021 cgcaccacgc cggtgaccgc gatggaagtc tcctgagtca gccgtccgct cgcctcccag
     1081 gcggcctcgg acacgtcctt tttggagagc accgcttgca ggtatccagt gccgtcccgc
     1141 agcgtgacga acgcgatctt gccgctcgag cgggtggtca tcacccaccc gcggacggtg
     1201 acggtctgac cgacgcggtc ggccaaccct tcaatgcgcg tctgttccgg cataggcgcg
     1261 gcatactagt gaccgtgctg tgcactgtca acgcgatcgc gtgcgcaccg gtcatccggc
     1321 acggacccga gagccagccc gtgacctggc cggtgtattt gggcaccccc gcgcacgaca
     1381 tcgacgccag cgaacacctc gaaggagccc ccaagtccgc gtggcgcgcc aacccgggac
     1441 gggagatccg gggcgcggcc gcggccggaa agggcgtgct cgccgtcggc accaccgacc
     1501 gggtggtcgc cctcctcggc accgactcgg gacaggtcct ctgggaccgc cgtctcgccg
     1561 ggacgatccg tggcggcccc ctgctcaccg gcaatcgcgt ctacgtcgcg acggaggaga
     1621 cgccgagcgg ccgcgtgtac gccctcgaaa tgcggacggg caagaccgtg tgggacgaaa
     1681 agaccggtgg cgtggccgcg gcgatggctc tcacgaagga cgggctcttc gcgggctccg
     1741 aaaccggagt cctgatggcc ctgaatcccg ataccggcgg catccgctgg cgccgccggc
     1801 tgagcggatc cattcaggtg gcgcccgtct ccaccaccgc cggactcgtc gtcgccacga
     1861 cggccgattc tctctacctg gtcgacccgg cgacgggagc cattctgcac cgcacgccga
     1921 cggcgggcgc ggtcctcggc acccccgcca cggacggcgc gcgcgtctac ttcgcgacgg
     1981 tcaccggcac gcttgcaggc gtcgacgccg cgacgctgga cacgctgtgg acgcgggacg
     2041 tgggctcggg ggtctacggc gctccggcca tcgcccggga tacgctcttc gtgctcacgc
     2101 ggacgggccg cctggtgcgt gtcccgctcg gctctccggc gtcggcaaag agcgtggagc
     2161 tcaacctcgt ctccgtcgcc ggcccgacgc cactcgccga tggcgtcctg gtcgtcgatg
     2221 ttggtggagt ggtccgctgc gtcgatgcgg acaccggcgc cgagcggtgg cgcgtgacgg
     2281 tgaccggacc cgtggagcag cctcccctcg tgtgggaagg ccggctcatt ctggtcgccg
     2341 gacgtggaga ggtggtggcg taccagtgaa gacgaccgcc gtgctcatgg cgttcaccct
     2401 ggccgcgcgc gccggctccg cacagacctc cctggtcgac tcgactccgc gcgcggcctg
     2461 gaagcacgcg gccgtgcact acggcaagtg ggcggccgcg gcgggcgctg tggcgttcac
     2521 cgcgctggcg atccagcaac accggcattc gaatgacgcc tggaattccc tgctcgcgat
     2581 ctgtaacaac aacagccaga actgcgccgt cggacccgac ggccggtatc gcaattatca
     2641 gtcggagtat ctctaccagc tggccatcta ctacgaccac cgggcgcgct ggcgcctcat
     2701 cgccggacag gtatccctcc tcggcgccgt cggcctcttc gtcgccgacc tccggggaaa
     2761 atccacgacg ccacctaacc agcccgttca tcccctcaag ctcgccgtga cgccgactcc
     2821 tgacggcgcc accgtcgccc ttcgcctctc tttctagccg tctagccgtc caggtcagcc
     2881 tacccgaaac agctcgaagc cgcgctcata gcgctgaagg atccgcgccc ggtaggccgc
     2941 gtgctcgggt cgcgtgagcg cggggtccga cgcaatgatc gcgccggccg cgacgcgcgc
     3001 cgcttccagg agcggcaggt cggtatccca atgcacgtag cggagcgcct ggccacccga
     3061 ttggcgcgcg cccgccagct ctcccatccc ccgctccttc agatccagct ccgcgatcgc
     3121 gaaaccgtcg gtcgtctccg tgaatgcttt gagccgaccg gccgcttcgg gcacgtcgga
     3181 aatgagtatg cagtgactgg ccgccgctcc ccgtcccacc cggccgcgca actgatggag
     3241 ctgcgccaac ccgaagcggt cggcgtgctc gatcaccatc accgtggcgt tgggcacgtc
     3301 gatgccgact tcgatgacgg tcgtcgcgac gagcagcggc acgtggttgt cccggaacgc
     3361 ccgcatcacg cgatcgcgtt cttcgggctt catccggccg tgcacgagac ccacgcgtga
     3421 ctcgccgaac gtcttcttga gggtgtccgc catggtcgtc gcggcgcgga gatcggcctt
     3481 ctccgattcc tcgatcaccg gatagacgac ataggcctgg cggcccgcgt cgatctcgct
     3541 ctgcaggaac gcatagatct tggcgcgcga cgcgtcggtc cggagcgccg tcttgatggt
     3601 cccgcgcccc ggaggccgct cttcgagtcg agtgacgtcc agatcgccat agagcgtgag
     3661 cgcaagcgac cgcggaatcg gcgtcgccgt gaggaggagg acgtcggggg catccccctt
     3721 ttccacgagc gcggcgcgtt gctctacgcc gaaccggtgc tgctcgtcga ccaccgcgac
     3781 gccgagtctc cggaacgaga ccgactcctg aatgagtgcg tgcgtgccga cgatgacgcg
     3841 ggacgccccc cgcgcgatcc gatcccgggc ggcgtccttc tcggggccgg tcaggcggcc
     3901 gatcaacaaa tctggacgaa ggtcgagggg ggcgagcagc ctggtcaacg tcgcgtgatg
     3961 ctgctccgcc aggagctccg tgggtgccat gatcgcggcc tggaactcgt tctcgaccgc
     4021 caggagcatc gcaaagagcg ccacgaccgt cttcccggtg cccacgtctc ccatgagcag
     4081 ccggtgcatg cgctgggggg cggtcatgtc ctccgtgatt tcgcggatgg cgtggcgctg
     4141 atcgccggtc agctcgaacg gaaggtgctc cttcaacgcc gtggtgagct cgcggcgcag
     4201 ctggaacgcg atgcccgtgc gggcccgctt ggccaggaag cgagcccgcg cctgcaccag
     4261 ctgctgatcg aagaactcat cgaacgccag gcggcggcgc ccgttctcgg cgtcggcggg
     4321 cgtgccgggc cgatggacgg ccgccagcgc cagccgtagc tccgggatcc cgaccttcgc
     4381 ccggagcgcc ggagggtgcg gatcgcggac gagctcgagg agcgcatcca gatgcggatg
     4441 gatgagcgcc cggatctgct tgtgcgtcag cccctccgtg gccgggtaca cggggaggac
     4501 gacgccctgg tcggggcccg cctctccggg ctcggcgaga atcacgaact cacgcggggc
     4561 cagctgtttg ccgtgatagt accgcaccgg cccggtcacg agcaggagct ggcccgcctt
     4621 gatggtccgt tcgaggaagg gctggcccgg ccacgcacac tcgagcacgc cgctgtcgtc
     4681 cttcagcacc gcccggaaga cccgcagtcc gcggcgtgtg ggaatgattc ccgtcgagac
     4741 cacgtggccc accccggtga cctcttcatt gatgcgcgca cgggcaaaag gcgtgacggt
     4801 ggtggcgtcg aggtaacggt gcggcacgtg atagaggaga tcgcccacgg ttcgcacgcc
     4861 cagtcgcgcc agtgcttcag cccgcttggg cccaacccct ttcaagaact tgaccggggc
     4921 gttgagctcc aggagcatca gtcgcggacc aggcggcgtg cgaacgcgtc cggatcgaac
     4981 agcgacagat cgtccaccgc ttcgccggtg ccgacgaacc gcggcaccgc ggccggcacg
     5041 gcgcgggcca gcgccacgac cgcgcccccc cgcgccgtgc cgtccagctt ggtgatgatg
     5101 gtccccgtca gcgggatgtc cttcgcaaag gcttcggcct ggcgcaccgc cgtctggccg
     5161 acggagccgt cgaggaccag gagcgactca tgcggcgcat cgtgcgcggg gtgggccttg
     5221 cccaccgcgc gcacgacctt cttcagctcc tcgatcaagc ggtcgtccgt gtgcaggcgg
     5281 cccgcggtgt cgatgatcac cgtgtcgacg ccgcgggccg ccgccgctct caccgcgtcg
     5341 aaggcgacgg cggcggagtc ccccgtgcca tcggcgctcg gacccacgaa cggcacaccc
     5401 aggcgctcgg cccagatgcg aagctgagag accgcccccg cccggaaggt gtccgccgcg
     5461 gcgagcaaag gcgttcgccc ctgtccctgg agacgcctgg caagtttagc cgccgtcgtc
     5521 gtcttcccga ccccattcac cccgtagagc aggatgacag tgggtgccgt cccagcccgg
     5581 aggagctccg ccggcacgcc attgccactg agggcccgga cctcccgttc cagcgccgct
     5641 tcgagctccc gatcgggcac cttttccaga cggcccagca gctcctcagc caatgccgga
     5701 ccgaagtccg cctcgagcag aatgcgctcg gcgtccgcgc gcgccgcctg atcgcggcgc
     5761 ccgaggacgc ggccgatctt gccccacagt cccatcaggg caaccccatc cggcgccgcc
     5821 acgtccattc cgccgcgaac accaggatgg ccagcgcgaa aagttcccac cgctcacgcc
     5881 aatcgagcac cacccggtcc gccgccgcgc gcccaggctg atcctcgagc gcggacccgc
     5941 gtcgccactc ctcggagtac ctatcgacca cgatcaagcc gtgcccatga ccagcggcca
     6001 acgcgtagcg ataggtcccc ggcgggagac gcacctgggc gtgaccgtca gcgccgaacc
     6061 gcagcgtgtc ggtggtggtt ccggaggccg acgcgaacgc gacgccgagg ggtttcggcg
     6121 atccgggccc cgaccagcgc cacgtgacgg gaagtccgtc gggcacgtcc agggaatccg
     6181 gacgggccca atcggtggcc gtcgagcccc ccgccagcaa ccagtcgacc aatccggcta
     6241 cggccgcgcg ataggcctgc tgggaagctc cgccccggaa atcccagcga tacaaccccg
     6301 ccaggttgac cacggcccgg cgcccccgcg acgactgcgc gacgaacacg acggggcgcg
     6361 cctggcctcg acgcgaccgc gtcgcttcca gcacgggcac cgcgccactg tccggaagac
     6421 tggggagcgc cgaaagcacc gggggcagcg aatcccacgg taccgtggac agcgcgccca
     6481 ccaggggtga gctcgaggag ggcgtgagat accaatcgcc cgccgagccg cccgcgctgt
     6541 tccaggtcaa gagggcgggc gcggagacct tcggccaatg tgacggatcg ccgacctcga
     6601 ccaccaagcg cgccccgcgg agagcacgcc gcacccgctc caccgatacc ggtgcgaggg
     6661 tgacgccctc gttccacttt ccggcagtga tctgggtgaa gtacttgacg ggcactcgag
     6721 ccacatcgcc cagggtgcgc gccaggaacg tcgcatccca gtcgggagga gcgccaagga
     6781 ccactgccgc gggttcggag gtcacttcga tcgggaagga cctgctgtcg tctcgcggct
     6841 ccgagtcccc tgcccactgt actccgaccg atagggcgga ccagcctgtc gggaaacgag
     6901 aaacgggaaa cgtgaggtcg acgttcacag tcccgctatc cggcagcacc acttttcgtg
     6961 aagtgaggat gcggtcggag agccggacat tcaacgtcgc ctcacgattc ccgattcccg
     7021 attcccgttt cccggatctt ccgtacgtca cgcgcagcct gaccgtatca cccggcgcca
     7081 cctgattcgg cccctcgacc gacgcgacga acgcatcagg agggcggccg tggcccacta
     7141 ccacgacgcg agcgcgggtg atgaggtccg acggcagcgc atcccggtcg tcgatggcgc
     7201 cgtcggtcac gatgacgatg gggccaccgc gcgcggcggc ggcggtcaag gcgggagcga
     7261 gactggaggc gccttcatgc ggtacggcgg tatcgaaggc ggcgaccgtc tcgccgaacc
     7321 ggagaatcac accgccgcga gcgagcgcgc gcgcggagtc gagcgcctcg gcccagagac
     7381 cgggttgggc actcatcgag agcgacgcat cgaggagtac gaccggtgcc gttccgccgg
     7441 gagcccggcg cgtgatggcg ggattccaga gcaggaggac gagacagccg actccagtga
     7501 cgcggaacgc caccggtccc caggtgttca ggccacgcac tggagcgctt ccgtcagcgt
     7561 gaaccgtccc tcgtaaagcg cacgtccgat gatgatgccc gcgagtccca gccgtttggc
     7621 gcgcgtcagc tcgtcgaggc ccgcgatgcc ccccgagacg atcacctcga ggccgagctc
     7681 cgccacgccg gcagcggcgt cgaggtcggc ccccgccaag gcgccgtccc ggggcacatc
     7741 cgtgtagacc acggtccgga ttccctgctt ctgaagccgt ctggcgagcc ccaggggact
     7801 ggtgccggga tcgactttcg ccgcgccgcg cggggcgagc cgtccgtcgg cgacgtccaa
     7861 cgccacgccg agcgtgctgg cgccgaaggc ggacagcagc cggtcgatgg tgcccgcgtc
     7921 gagggcagcg gccgcgccga cgatcacgcg cgtcgctccc caggtcagga cctcgcgcac
     7981 gtccttctcc cccccgagtc cgccgccgac ctgcacccag ccgagccccg cgtgcaggag
     8041 ctgccgggtc acggcgtgat tctcgccgcg gcgatacgcg cggtccatgt ccacgtagtg
     8101 gagccactgc gcaccggcgg ccacgaaggc gcgcgccacg gcgagcgggt cgtcggcgcc
     8161 ggccggaacc gccggggcgc gcgccacccg cccaccggcg acgtcaacgg cgggatagag
     8221 gtccatcgga tgttcggaag ctaatccccg gccgaagcga ggctggcgac gggagccggc
     8281 tcgagcagcg acaggagcgt cgtgcgcacc gcgttgaacg aggcctggag ggatttcggg
     8341 accggcgtgc ccgcacccat gtccttgcga ttcgggttcg tcgcctgccc attcacgagg
     8401 aactcgtagt gcaggtgcgg ccccgtggac aggccggtgg atccgacgaa gccgatcgtc
     8461 tccgattgcg tcacgcgctg gccgacgtgc agccctttcg catacgccga gaggtgtccg
     8521 tagatggtct tgatgccgtt ggcgtgccgc agctcgatca tgcggccgta gccgtcggct
     8581 tcgtagacga gccggtagac cacaccatcc gccgtagcgc ggacgggcgt cccataggcc
     8641 gccgagaagt cgatgccttc gtgcttgcgc caccggtgca ggatcgggtg ataccggttt
     8701 ccgaatccgc tcgagatccg ccggaactgg agcggcgcgc gcaggaaggc gcggcggagc
     8761 gatcggccgg tttcatcgta ataccccgcg cgtgccgagt cgacgcccgc gaaccggaac
     8821 gcgtagttcg gagtcttggc cgcgtccact cgggcggcca ggatgcgccc gaaccggcgc
     8881 tcgcccgtgg gcgattcgag ccgctcgaac aggatgtcga cccggtcacc cgcgcgcacg
     8941 tcccgggtga agtccacttc ccagtcgtag acgtcggcga tggcccacgc gagcgccacg
     9001 cgctggccgc gaggcaggac gctgtcggga atcgcggcgt cgaggccgtc atacaggttg
     9061 tcggtaatgg caccggtgac ccgcacccgg gtggacgtcc aggcgatgga ctcgacttgc
     9121 tcgttccaca gcacgccggt ccgggtgagc cagatgcgct gttcgggggc ggtccgcacg
     9181 ctcacccggt ccgcgatcga atcggcgatg ggacggcgga acgcgaagac cagacccggg
     9241 cgcaggcggc gggcgtcgag aacgtgcgcg gcggaaagca acgcgccgta ggcacgaccc
     9301 gtaatcccgg cgcgctggag caggtcaccc agcgtctcgt cacgccggag ggtgtcggag
     9361 tactcctgat acgcggcggt caccaccacc ggcggcgccg cgagtggctt gtgcatccac
     9421 gcacgcgcga tcagtccccc gcccaccagg accaccacgc cggcacctgc cagcgcgctc
     9481 cgaaccatct gcggcctcgt catcagtcca tctggcgccg gatgaaggtc gggatctcca
     9541 tctcattcac ttccgccgtg gtagcgggcg gcggcgcact cggccgcttc ggctgaacct
     9601 cggtgggctt cgcgtatccc ggcggagggg gcggcgtcgg acgcgtggcg ggccggaacg
     9661 gcagcacgcc ggggcccggg cggccggcgg tggcggcctg ggccgcggct tcaccggcga
     9721 cgccggcttt gtcgaacccg gtggcgatca cggtgacgcg gacttcgccc tgcatggcgg
     9781 gatcgttcac ggcgccgaag atgatctccg cttcctggcc cgcggcctcg tgaatcgcct
     9841 cgctgatgac ggtgacctcg ccgagggtga ggtcttcacc gcccgtgatg ttgatcagca
     9901 cgccggtagc acccgagatc tggatgttgt cgagcaacgg tgagctgatg gcctgctgcg
     9961 ccgcttcgag ggcccggttc tctccgcggc ccaccccggt gcccatcagc gccgagccgc
    10021 cgttctgcat cacggtgcgg acgtcggcga agtcgacgtt cacgatgccg gtcacgctga
    10081 tgagcgagct gatgccctgg gtggcgtgca ggagtacttc gtcggccttc ttgagcgcgt
    10141 cctggaacgg aatccctttc ccgaccacgg cgagcagccg ctcgttgggc acgacgatca
    10201 tcgtgtccac gtgcttccgc atttccgcga tccccatctc ggcctgcttc atccgtttcc
    10261 ggccttcgaa gaggaagggc ttggtcacga tgccgatggt gagggcgccg aggtccttgg
    10321 cgacctggga gatgatgggc gccgcgccgg tgccggtgcc gccgcccatg ccgcaggtga
    10381 cgaatacgag gtccgcgccc tggagcgcgt ggaggatttc gtcgcggttt tcctcgatgg
    10441 cctggcgtcc gatttcgggg cgtgcgccgg ctcccagtcc gcgggtcagc ttcttcccga
    10501 tctggacctt gatgtccgac ttgttcttga gcagggcctg cgcgtccgtg ttcaccgaaa
    10561 tgaactcgac ggcctgcacg ccctcgtcca ccatccgatt gagggcgttg ccgccgccgc
    10621 cgcccacgcc gacgactttc atccgcgcgt tctgcgccac cgtttcttcg agctcgaaga
    10681 tcatggttgc cccggctccc tgatcaaaag aagtcctgca gccatcgctt gacgggtccc
    10741 aggagcttgt ccattcccgg accggtcacg agtgagcctg cccccccacc accaccctgc
    10801 ccgccactca tcgcgccctg caccacgcgc cgggcggcgt acatcgcgag cccgaccggc
    10861 acggcgtagc gcggcgcctg cacactgtcg atcaatccac cgagcccccg ctcgggaatt
    10921 ccggcgcgca ccggcatcgc gaatacctcg cggccgagct ccacgattcc cggcaagtgg
    10981 gcggttccgc ccgtcaggac cacgcccgcc ggcagcagct tcggatcgtc gtagccggcc
    11041 ttggtcagct cgccgcgcac cagctgcagg atttcgtcca tgcgctggtg gatgatgtgc
    11101 gccaccatgt cgcgcggcgc ctgccgcgcg ccctgtccgg gggtcgacgg cagatcgacc
    11161 atctcatcgg gattcaccag cggctgatac gcgctgccga accgctcctt cacccgctcc
    11221 gcgtcggcct gcgtgagcga cagtccctgc accagatcgc tggtcacgaa gccgcccgcg
    11281 aacttcagcg aggcgaggtg ccggatcttc cccttctgga agatcgcgac gccggtcgac
    11341 tgcccgccga tctcggccag ggcgactccc agctcccgtt cgtcatccgt cagcaccgcg
    11401 tgcgacgccg cgagcggctc cagcacgatg tccgccacct tgaacccggc gcgctccacg
    11461 ctgcgccgga ggttctgcgc cgcggtggcc tgcaccgtca ccaggtacat ctccacttcc
    11521 agccgcgtcc cggtcatgcc gaccggatcc gagatctcgc cctgctggtc gaccttgtac
    11581 tcctgcggaa tcccgtgcag aagctggagg tctcgtccca gcgcgaccgc gcgcgcgact
    11641 tcgttcgccc gctcgacgtc ggggccggcg atctcttccc cccggatggc caccaccccg
    11701 gaggacgtcc gcgccgacac gtgctctccg gcgatgccgc aggtcacctc ggtcacgctg
    11761 gcgccggcca tccgcgacgc gtcccgcatc gccgccgtca ccgagcgggc cgtctcctcg
    11821 atatcccgca ccatcccgcg ccgcacacct gtattcttgg cgacgccgac acccaggatc
    11881 ttcacgaccg gaacgcgggg cagatctccc gcaatctcgg cgatcaccgc gcaggtcttg
    11941 gtgctgccga gatccagtcc cgcgaccagc cgtcgttcgt tcttgctcac gacgacgcct
    12001 ttcgccggac cacgatctgc ccggcaaatc gcgcgtccag ctcggcgtac gtgcgtcctt
    12061 tggccgccag atcctgctgc accagcacca ccgcctgaat gacctccggt tgaggatcgg
    12121 ctccgaggag caagcgccgg tgggcgcccc cttcatccaa tgcgaccgcg ctccgggaaa
    12181 tccgccgcgc cgaggtgatc gactggaaca gcacgggatc caccgcccgg accaggccca
    12241 gcaccgacgc gagcccggaa tccgcgctcg ccgccaccgg cagatccagg accgtccgcg
    12301 ccgggtcgaa cggcagtggg tggcccgccg cgtcctccac cacgagcccc cgcggtcccg
    12361 gcacgaatgc cgccggatcc acctcccgca cgatcactac cagcgcgccg gggaggtggc
    12421 gcttgatcct cgcgtccgcc acacccggca gggcccgcac ccgcgcttcc agcggcgccg
    12481 tcggatcgaa cagactggcc tcgcgcgaca atccgagggc ctgaatcacg gcttccggtg
    12541 acagattccg cacgccgacc agctcgacct ggcgcagccg gaagaactcc agatgccggc
    12601 cgacggccgg cccgccgacc cacaacccgc cgagcgcccc caggaccacg agcggaatcc
    12661 acactcgccg gtccttcaac gcctcgagta tcacactcac cgcggcgccc gttcccggcc
    12721 ggcgctgcgg gatttcgtga gaatctcggg acccacgcgc gtcacatcgc ccgcgcccat
    12781 cgtcagcagc aggtctcccg gctggagcag tgccgctacg tccgcggtga gcttcgcccg
    12841 gtccgcgact ttcacgacgt ccgcgccggc cgcccgcgcc gcgtcgatca ccagatccga
    12901 cgtcactccc gccatcgggg cttcgcgcgc gccgtagatc ggcgccacca ccgcgacgtc
    12961 ggccgcggcg agcgcctgtc ccaacgcctt cccgtgcgcc gcggtgcgcg aatacaggtg
    13021 cggctgaaag accgccacca cgcgccgatc gggaaacgcc tgccgggcac cggccagggt
    13081 tgccgcgact tccgtggggt gatgggcgta gtcgtccacc accatcacgc cgtccacctc
    13141 tcccagtcgc tcgaaccggc gcgctacccc gcggaactcc gcgagggccg ccgcggcgga
    13201 tcgcacgttc gcgccgaccg cgtgcaccgc cgccagcgcg acaccggcgt tgcggaggtt
    13261 gtgcgcgccg ggcacctgca acgtcagcgc gaccgcgtcg ccattgggca acttcacgcg
    13321 cgccgtggat ccggtcggcg agaagtgtgc gtcctgtatc ctcacgtcac ccgaatcggt
    13381 cccgacggtc cacaccggca ccttcagccc gtggaccact cgccgcgcgc ccggatcgtc
    13441 ggccgaagcg atcacgcgct gcgcccgccc ggcgaactcc gcgaaggccc cttcgagcgc
    13501 ctcgatgctt ccgtaacact cgagatgatc gggctcgacg ttgttgacga ccgcgaccgt
    13561 cggctgcaaa gacagaaacg ccttgtcgta ctcgtcggcc tcgaccacga agagctcgga
    13621 gctcccctgg cgggcattgc caccccatcc agcgacacgg ccgccgacga gtcccgtggg
    13681 atttcgtccg gcggccgcta agccctccgt caccatgacc gtggtggtcg tcttcccgtg
    13741 cgttcccgcg atcgccacga ccgtcccacc ggcgacggct tcccccaggg catcagccct
    13801 ccggatgacc gggattccga ggatgcgcgc ccgctcgacc tcggggtgat gcggcggaat
    13861 cgccgccgtc accaccatcg ctcgcgctcc ctccaggtgc gacgggtcgt gccccctgga
    13921 gaccggaacc cccagcgcca caagatcttg ggctccagcc ggatcctggt cgcacccggt
    13981 gatctgaacc cctcgccgtt tggcgataag cgccaaggcg ctcatccccg cgccggcgat
    14041 acccataaag tggactgggc gggggtcccc gggtgcaaac aaggtcatct gtagtgggct
    14101 aatataggga atctactaca cttgcgccag ctacgagacg agtgtcaaca atctggacac
    14161 cacgtcccgg gacgccgtcg gtcgaccccg accgagtgcc gcgcgcgcca tcgactgccg
    14221 ccgagccgag tcggaaatca actgcgtgat cgccgtggcg agcgagtgcg gcgtgagcga
    14281 cgactcctcc atcaacacgc tcgcgttcgc ttccgccatc gcgcgcgcgt tctccagctg
    14341 atgattcgcc gccgcggtgg gcagcggaat cagcagtgag ggtttccccc acgcgcacag
    14401 ctcggcgatc gtcagagccc ccgcccggca gacgacgaga tcggtagccc ggtagatggg
    14461 cgccatgggg tccaagaacc cctgcaccac gactttccct gatcgggcgt accccgagag
    14521 ccccgggtaa ttcgcgaccc ccgtccccca gatgaggttc atgccctcca ggtggccgtg
    14581 ctcgagcacg ccggccaccg cgtagttgat cgcacgcgcc ccctggccgc cgccgaaaac
    14641 caggagtgtg gggagcgccg ggtccaggcc gagggctttg acggcctcgg cccgatcccc
    14701 cggctgcgga ggcgtgatgg ggtttccgaa cgtgaacacc tcggtgcggt ctccgggtcg
    14761 cagccgcgcc tgggcctcgg gaaaccccag gtgaatctgc cgcgcccgtc cggagagcca
    14821 ccgcgtcgcg atgccgggac gcgcgtcgga ctcctgaatg gccgtgggaa tgtcggaccg
    14881 ctgtgcgtga tacacgaccg gtccggaggc atacccgccc gtaccgacga ccagcgtcgg
    14941 ctggacctcg tccaggagcg cgccgatgcg gcgccagagc cgcaccgcga gtacgggcca
    15001 ctggaggttc ttccaccatt gccgccgata gatgggctgc aggggcagga gcgtaaatgg
    15061 atagggatgc tgcggcagga tctgcgactc gattccccgc tcggcaccga ccaggtggat
    15121 ttcaaggtcc gggcgcatct gccggaacgt gtccgccagc gccagcgcgg gggtgaggtg
    15181 accgcccgtg ccccctcccg aaatcagaat cttcacttgg acgcgcccgc cggggatgtc
    15241 gcacgccggc gtccggcggt cttgcgctga ctctgctgtc cgtgaccgat gctcaccagc
    15301 acaccgatcg cggccaggga gacgaagagg ctggtacggc cataggaaat gagaggcaac
    15361 gtgatcccgg tggccggcat gacggcgagg ctgacggcga tatggaggac cgccgtcacg
    15421 ccgatcaacg ccgtgagccc ggtcgcgagg aattgtccga aggggtccgg ggcccggcgg
    15481 gcgatgcgaa agcccagcca gcagatcccg gcgaagcaga ggatgatcac cgtgaccccg
    15541 atgaagcccc actcctccgc gatggtcgag aaaatgaagt cggaatatgc atacgggagg
    15601 tatccgagct tctgctggcc ctgtccgaat ccgaccccga tgagtctgcc cgcaccgatc
    15661 cccaccagcg actggtgcac ctgcgactgt gcctcactgg gtgcggtacc ggagctgaag
    15721 aagtgcgata cgcgcgccag ccggtactgc gcgctcgcca cggcaccgaa gagcacggga
    15781 gcggcggcga tcccgatcag caggaagtgc ccgatccgcg cgcccgcggc gaacagtacc
    15841 gtacccgcga gcatggtgag cagcagcgcc atcgacagat tcggctcgag gaagacgagg
    15901 ccgatgacgg ggagcagcac gacgaagaac ggcaggagcc cgaacttgaa ggtgcggatc
    15961 tggtcgccct tcttggccgc cagcatggcg gtccagcaga ccagcgtgaa cttcgccagc
    16021 tcggagggtt gcaccaccgt gaagccgagg ttcacccagc gccgggcgcc attcaagatc
    16081 ggcgcgatgg ccctggtgaa tggcagtacg ggaatgatca gcaagagcag tgtcaggccg
    16141 agcagaggcc aggcccaccg ttgccacacg tggtagtcgg tgcgggcgag aatcgtcgcg
    16201 cccaatcccc cgatcgccgc gccgatggct tgtttgaaga aatagctggc accgacgacg
    16261 cccccctgcg tattcacgag gctcgacgca ccgtacacgg ataccaggcc cacgacgatg
    16321 aggatggcgg cgaggatgcc gaggaggcgc gtttcccagg cattctcctt cacaggccct
    16381 ccgcgaattg ccggaaggtc gcgccacgct cttcgtagtt cttgaactga tcgtagctgg
    16441 agcaggcggg ggacagcagc acggcgtcac cgggccgcgc ggccttctgc gcgcgcgcca
    16501 ggacgtccgc gaacgtagtc ccccgctcga tcgggacctg gccctccagg tctttcgcca
    16561 caatcggccc cgcctcacca taggcgatga cgagccggca ctgcggcttg aggagtggcc
    16621 cgagccgcgt gtagggctcc cccttgtgcc ggccgccgag caggagcacg tagggacgag
    16681 tcatggcttc gacggcgact accgtcgagg cgatgttcgt cgccttggag tcgttgatcc
    16741 actgcacgcc gtcgacctcg cggaccggct cgagccggtg tggcagcgcc cgaaaggact
    16801 tgagaccttc ggcgatcgcc gacggagcga ctcccgcccg atggaccgcc agcgcagccg
    16861 ccaacgcgtt cgccacgttg tgagcgccga ggagattgag ggcctcgcga tggaggagga
    16921 cgccgttctt gagcatcagc gcgccggccg cggcgtcgta ccaggcatcg gccttccact
    16981 cgagggagaa ctgcagcttg gtccccgtga cgctcttcac catgtcgagg acggcccggt
    17041 cgtcgccatt caccacccag gcgtttttcc cgtgggcatt ccggaagagc atcgctttgt
    17101 cggcgtagta ctcatccacc gaggcgtacc gatcgaggtg atcgggagcg aggttcgtca
    17161 ggacgccgat gtcgggggcg aagtggggcg agtcgtggag ctggaaggaa gagacctcga
    17221 tcgccaggaa ctggggatgc ggggacgaca gcgccatgtc ggacaccggt ctcccgatgt
    17281 tccccaccgc ctcggccggc aggcccgccg atcgcagcaa atgggcgatc agggccgtcg
    17341 tcgtggtctt cccgttggtg ccggtaatgg cgatgacgcg cgtatcctcg agggcgcggc
    17401 accccacgtc gagctcggag acgatcggga tgccggactc ccgcgccgtc ttgaggggag
    17461 gtgcatcggg tggcacgccg ggcgacacga cgacgccggc ggcgtgctga atccgcgcga
    17521 ggttgtgatg cccgagttcg acctcgacac ccggaagcgt gcccagggcc cgcaccgcgt
    17581 cttccgcgta ggggtgctgc gagccgtcgg acgcgtagac cttcacgtgc tcgcgggcca
    17641 ggagccgcgt ggccgcgacc ccgctcttcc ccagcccgat caccgcgacg gtgccgtggc
    17701 gccaggattc ggggagcttc atcgcacctt gagcgtcgcg agcgcgatgg ccgcgcacag
    17761 cacgcccagg atgtagaacc gcatcaccac cagcggctcc gcccacccca ttttctcgaa
    17821 gtggtggtgc aagggcgcca tccggaatac ccgatgggca tccgcgtact ccttgccccg
    17881 ccgtttccgg ttccagcggt acacgttgac ttgaatcatc acggacagcg tctcggcgac
    17941 gaacacggct cccgcgatca gcaggagaaa ctccgccttg agcatgatgg cgatggcgcc
    18001 gagggccccg ccgatggcca gtgaccccgt gtctcccatg aagaccttcg cggggtgcgc
    18061 gttgaaccag aggaatccca gactcgcccc aaggagcgcg gcgcagaaca cggcgagctc
    18121 acccgcgccg ggcgaataga gcacgcccag gtacttcgag tagtcggcac ggccgaagac
    18181 gtacgcaaaa atggtgaacg atcccgcgac gatggcggtg agaccggtcg ccaggccgtc
    18241 gagaccgtcc gtgaggttca cggcgttcga cgaccccgtg acgaccaggg tcacgaaggc
    18301 gatgtagatc ggccacggca agaccagaaa ccagtacttg aagagcggca cctgcgtcgc
    18361 ggttctccat tcgagaacga agggccacaa gacgaggaag gtgccgagcg cgatcccgaa
    18421 ggtcacctgc cccacgagct tccaccgggc gacgaggccc tgcgatttgc cccgcacgat
    18481 cttcaggtag tcgtcgagaa agccgatggc gcccatccag agcgtggcca ccacggcgac
    18541 gagcacatag cgcgtatcga gccgcgccca cagcagcgtg ggaatgacgg tggcgatgat
    18601 gatgatgaga ccgcccatcg tgggcgtgcc ccgcttcccc tgatggctcg ccggcccctc
    18661 cgcacgcacc acctgtccga cgttgcggcg acgcaggatg tcgatgatcc aggggccgac
    18721 gagaaacgcc acgatcaagg ccgtcgccgt ggccccggcg atccggaacg tgatgtagtt
    18781 gaagagattg aagaggatgt acttcttccc gagtggcgcg aacaggtgat agaacacggc
    18841 ccgatttccg ccctcagttg aggtggcgca gcacccgttc cagcgcgact ccccgtgacg
    18901 ccttgagcag caccacttcg ttgcccgtga gccgctgctt cagcagcggc cccagccgct
    18961 ccgcatccgg tgccacgacg agacggtcct tgagcgctgc cgcaaacggc gagaaggcgg
    19021 cctcgaacgc gccgaccgcg cccaccagcg cgggcgccgc ggcgacgatc tccgccgccg
    19081 ccgcccgatg caatttgtca ctctccgcgc ccaactccaa catcgtcccg accaccaccg
    19141 cgagcgggcg agagcgcttg tcggccagcc aacgggcgaa ctcgacggcc cgccggagcg
    19201 atccggggtt ggcgttgtag ctgtcgtcga tcaccgtcag gccgcgcacc tccgtgaccg
    19261 cgccgcggcc tccgggcacc cgtgcggccg tcagcccctg caacgcgtct ccgggacgga
    19321 tccccgcctc ccgcgcgacg gccacggcga tcgcggcgtt gtcgatctgg tggaaccccg
    19381 tcacgggcac ggtgacctcc cgcccgcccc accggatgcg cgggtgaccc gactcgtcca
    19441 ggtcggccgc atcgggatgc atgtcggtcc cggcgccggc gccggcgacg accgtccggg
    19501 tcttcgcccg cgcggccaca gcgagaccag gaggttccgt gccgacgacc gccacttccg
    19561 ccttctccac gagcgacacc ttctcacgca tgaccccctc gagggagccg aagccctcga
    19621 cgtgggcgta ccccacgttg gtcaccacgg cgatcgtggg ctcgatgatg tcgcgcagcc
    19681 tggggatctc ccccgggaga ttcgccccgg cttcgatcac catggcgttg gtcccggcgg
    19741 gggccgcgag aatcgacagc ggcacgccga ccaggttgtt gaggttcccc gcggtcgcgt
    19801 gcacgtgata gcggacgccc aatgcggctc tgatgagctc cttggtgctg gtcttgccgc
    19861 tcgatccggt gaccgcgacc accggcgacc cgtcggcgag ccgccgccgg cgggcccggg
    19921 ccagagcgcc gagcgccaca agcgtgtccg gtacttcaat gaaggggagg ccttcgatgt
    19981 tgggggtgcc gcgacggacg accgcggctg cggcgccccc cgccctcgct tgatcgagaa
    20041 acgcgtgcgc gtcgaagtgc tcaccggcga gagcgagaaa caaggcgcca ggagtcaggg
    20101 accgggtatc agtcgagacg ccgctgaagg ggccagatgc cgtcaccttc actccgagcg
    20161 cgtcgctgac ctggacagcc gtccaaggct cgggaggagc ggccggtgtg ggggtctcgc
    20221 tcttcacgat gcccccagct cccggaccac ctggcgttcg tcgaacggct gcttctcgtg
    20281 gccgatcacc tggtaggtct cgtgtccctt cccggcgaga atcacggtgt ccttggcgtg
    20341 cgcgagcgac agcgccagag cgatggcttt ccgccggtcg gcttcgcggt gatgcgggcg
    20401 cccctgcatg ccctgctcga cgtcgtcgag aatccggttc ggatcctccg tgcgcggatt
    20461 gtcggaggtc gcgatgacca cgtccgcgag gcgggacgcg atgtccccca tcggcgcccg
    20521 tttcccgcgg tcgcgatccc cgccagcccc gaagaccgcg atcaccttcc ctgccgtgag
    20581 tggtcggacc gtttccagcg cccgttcgag ggcgtcgggg gtgtgcgcat aatcccgaag
    20641 caccacgcag ggcctggagg cgatgcgctc catgcgcccc ggtacctgcg gcgcgtgcgt
    20701 cagtcgctcg gccacgccgt cgatgggggc tcccagcccc aatgcgcacg cggcgacacc
    20761 gagggcgttc gccacgttga acgcgccgag caagggcagg ttcacgtcag cgtggccctg
    20821 cgggctcacc agggtgaacc gcgatcccgt cccgtcggtc ttcacccgct cggcccgcac
    20881 gtctcccttc gtctcaccga aggtgatgcg ccggtggcca cccggcagat tctgccatgc
    20941 cggatcttcc gcgttgacga cctggatgcc gtcgggggcg agataagtcg acagcttcgc
    21001 cttggcctgg aagtagtgct cgaacgtctt gtggtagtcg agatggtcgc gggtcaggtt
    21061 cgtgaagatc gcggcgcgat agaccaggcc gtccaccctt ccctgatcca gactgtggga
    21121 cgacacctcc atggccgccc cgcgagcgcc ccgttcaacc aacagggcga gcgtttcctg
    21181 caggtcgatg ggtcccggcg tcgtcaggtt cccggcttcg ctctcgaccg gcgtgccgtc
    21241 gggcgcgaac gcgcccaggg taccgatcga ccccgtcggc cacaggagcg acaggacgtg
    21301 ccgccccagg gtcaccgacg tggtcttccc gttggtgccc gtgactccca cgaggttgag
    21361 ctttctggcc ggatacccga accacacctc ggcggccact gccgcggccc gtcggccgtc
    21421 tttcacgacg acctggggca acaatcccgg ctgggctgtc tcgacgagcg cggccgccgc
    21481 cccctgctcg gtcgccttgg acacgtacgt gtgtccgtcc tgaatggccc cgcggacgac
    21541 gcagtagagc atgcccttct cgagcttgcg ggtgtccgcg gtgaccccgg taatggcggg
    21601 cagcactacg ggcgcgtgga ccaggagtcc gcgctcccgc agcgcggcga cgatgtcggt
    21661 caactcgatc actgcggtgt gtctccaaag acggtaacga gcgatccggc ggcggcctct
    21721 gcccccgcgg cgggccacgt tttctggacg ctgccccacc ctttgatcac cacgtggaac
    21781 ccacgacggt gcaagaggcg gaccgcgtcc cgcagggtcc gtcccgagac gtcgggcacg
    21841 gggcgcggag gcgcgggggc cgcggtgtca tgcgcgggtg gccaactcac cacgtaggga
    21901 gcgagtccgg ggtcttcttc gagctccccc acgcccgcgg ccggagcgac ggcggagagc
    21961 cgggcgcgat cgagcgcgac ggtgcgggat gccagcgcct gctcgagcat cgcgcgggtc
    22021 accggcgcgg cggtctcggc cgcgaagtag ctccccttcc ggggattctc gaccttcacg
    22081 accaccacga gctgcgggtg gtcggcggga aacagggccg caaatgacgc attgtaggcg
    22141 cccgccttgt agtggccatt cacgacctgc ctcgaagtcc cggtcttggc cgccagcgtg
    22201 aacgtcgcga gggccgcgct ggctcccgtc ccgccgctcg acaccacgcc cgcgagaagc
    22261 tgccggagct cccgggccac cgcgggtgac acgacccgcc gaaccggttc gggctgatgg
    22321 cggtagagga tgtcgccatt cggagcgcgg acctcacgga tcagggtcgg ctcgagcagc
    22381 accccgtcat tggcgatcgc cccatacgcc gccgcgatct ggaggggggt caccgacacc
    22441 tcgtacccga tggcgagcga ggtcggcgtc ggccgggacc actccttcgg caacgcgagc
    22501 cggcccgagg cctcggcggg aaattcgatc cccgtgggcg tccccaggcc gaaatcccgc
    22561 agcatcccgt attcgtcgtc gtgatcgagc cgggtcacga atttggccat cgcgatgttg
    22621 gatgaaaccc ggatggcgtc ggccagattc agcatgggca acgggtggtc atccacgagc
    22681 gtccggtcct gcattttgta cttcccgttt tccccggaca cccggtcggt caggctgatg
    22741 cggtgatcgg cgatcagcgc ggcggcggtg aagatcttgg cgaccgatcc cggttcgtag
    22801 gcgtcgatga acgccgatgg ccgcgagacg ccgtcccggc gctcggaagc gaccgccagc
    22861 acttcccccg tcgccggatc gagcatcacg acatcgccgc cgtccgcgtc catcttctcg
    22921 agcgcaagac gcaacgaccg ctgggcgatc tcctgcagct cggcgtcgag cgtcagcacc
    22981 acgtcgttcc ccggcaccgg cgactggagg cgacgtgccg gcgagtcgta ctcccggccc
    23041 gcgcggtcct tcagcacgac ggcggcgccg ggggtccccg ccagcagcgt atcgagagtc
    23101 cgctcgagtc ccgagggctt ctgcccccct tcgcccgcgc ggccgatgaa cgcgcgcgcg
    23161 aagtcaggtg agggatagaa ccggttgagc accggctcga ggtgcacgcc gcgaacgtcc
    23221 cgcagcccct gcaccgtacc cgccgtgaac ggtcccgcga agtaggcgta gtgccggctc
    23281 agctcgtcct cgacgcgcat gagcggcagg tgcagcacgc gggcgatggt ttcgggatcg
    23341 tgatcgggat cgcggagctc gttgggtgcg atgccgacgt gatacgtctc ctgtgtcagc
    23401 gcgatcggtg tgccgtgccg gtcgaggagc gcgccacggg gcgcttccag aacgacatgc
    23461 tcggtgcgcg cctgcttcgc ctcggcggac cagcgcgcgc cctggaagag ctggacctgg
    23521 gcggagcgga cgagcagcgc caggagcagc agacccagcg agccctcgat gaatgagacc
    23581 cgggtggtca gcttcgccac gtcagggcgc gctcgtgtcg ggaacgggct ggatcacgat
    23641 ttcggaatcg gcggggagac gcaggcccaa cgcctgggcc cgggggacga gcacggagcg
    23701 gctcgcggcc tggcggatcc ggcgctcgta ctccgcgcgg cggctctgga gggaggacag
    23761 cgaatcgcgg gcctgattca aggcccgggc gatgcggagc gccgcggtct gcctggccac
    23821 cacggatccc gcggccacca gaaacgccaa cagccagatc atcacccagt gccgaccctt
    23881 gagcaccact caggccacct cgaagtcgcg aaagtgcgcg gagcgggacc gcggattcgc
    23941 cgccatctcc gccggaccgg gtgtgatcgc ctttcgggtg cgcagcgtcc cgagcgcgtg
    24001 tccgcggcac gtgcagaccg gttgtccagg tggacagatg cactcccggg cccactcgcg
    24061 gaacgcgtgc ttcacgatcc ggtcctcccc tgaatgatac gctatgacgc ccaaacgacc
    24121 ccccggggtc agggcgtccc ggaaggcggg gagcgcggtg gtgagctgct ccttctcccc
    24181 attcaccgcc atgcggagcg cctgaaagag tcgggcgaag tccgggggac ctgacctgga
    24241 ccccagcgcc tcgcggatcg cgttcacgaa gtcgtcgctc gtcatgaagg gtccgcgctc
    24301 gcggcggcgg gtgatgctgc gggccaggcg ccgcgccttc ggctcgtcgg cgtcctcagc
    24361 gaacgccttc gcgagcctct cctcccccca ggtattcagg atgtccgcgg ccgaaacgcc
    24421 cacatcgggc gtcattctca tgtcgagcgc ggcgcccggg cggaatgtga agcctctggc
    24481 ctgcgcatcg agctggtgcg aagaaacgcc gaggtcgagc aggatgcggt cgggctggaa
    24541 ggcgcggacg gcgtcgaggg ttttgggagc tccgaagggc gccgccagcc aggtcacgcc
    24601 gtcatcgacg atgcgcgcct gggcttcggc cagggcttcc gggtcccgat cgatcgccag
    24661 caccagcgcg cccgagcccc tcaggatgac cgtgtgcccc ccgcccccgg cggtggcgtc
    24721 gacggcgcgc gcgcttcccg cggcccagct gcggatgacg tcggcgagga cgggatcgtg
    24781 gaagctcatg ggctcctgaa aaagacaccg gctccgcgtg ccgaagcagg cgaagccggg
    24841 aatagcgtcc cgcgaagcgg gttcaggcgg ggctgacggg gctcgaaccc gcgacctccg
    24901 gtgtgacaga ccggcgctct aaccagctga gctacaaccc ctgatatcga gcgcgggaat
    24961 gtagacgggc gccgaggtca tttcaatggc tcagaagttg gtgcacttga atgccgcctt
    25021 cacctgcggc atggccttgg cgtacttgtc cagaattccg agcatctgtt gagcgaacga
    25081 cggggacacc ttgcccccca ggaagagtgc gtcccgggtc ttggcgatcc gctcgttgat
    25141 ttccttcgcc tcggagcagc tcttgtgatc cgtcatgtcc ttgtacggtc cgccgaggga
    25201 caggacggac gacacgccgt accagaagtt tccctggatg cggatctgct gcttggctcc
    25261 cgcggtatcc gcgggccccg cctgcgggat cagggccagg aggtccatgg accaggggta
    25321 ggcgcggtcc cagagctggg cgctccgcgc gatcccggac gccgcctgca gcatcaaggc
    25381 cgcggcgttg atcttcagta ctgaatccgt cccggtgatg gcgggctgaa ggtacggctt
    25441 cgcctggtcg aaccggtcag cgtacgcggt ccggtaggcc agcttgtgcg cgctgtcgcc
    25501 cacggccttc gtgctgtcgt agacgttgtg attgatgatc gtggcggcca cgaggagcgc
    25561 cggacgagcg tcattgggcg tgagtgccgc gcacgccttg taatcggcga tggcggaatc
    25621 cacctgcccg atggcttcga gcgcttgacc gcgaatgttg cagaatccgg ggttcttcgg
    25681 gaaatccttg aacgcaatgt tcgtgtagtg cacccacgcc tgcgtatcgg ggagcgcgtt
    25741 cgccgccccg atcatgatct tgagctcagt cgagtcattc agcttggccg cggagtcctt
    25801 ttcctccgcc gcgccgatat ccagcacgca gcgccactgg ctcccttcga tgcaggagcg
    25861 gcggagcatg tccttcgcgg cggcgttggt cggggcgtgg gcggtgacgg tcttgagcag
    25921 cgccgtcgcc ttcggatacg catggttctt catcaattga tcggccacgc tcagctggag
    25981 cggaatgttg tccggctcca cggcggccca gctctgcagg gcgtcgagcc acttcagcgt
    26041 gtcgcccttc tgctggtagg agcccgcgat ccggctccag gcggtggtgt tcatcgaatc
    26101 gcccttcagc gcccggtacc cggccgcgat gatgctgtcg gggggctgcc gctgggcttc
    26161 atagacggtc aggacgcaga ggtgcgctgc ggtaaggttg gggtcgatcg cgaaggcctt
    26221 cttggccgac tccaaggccc cgttcaggtt gccatgcgag cggcggtcgt cgcattcgcg
    26281 cgcgaattcg gccgccttga tcaccgtcac gaggcgctgg cccagcgatt cagccaccgc
    26341 cggcggactg gcgcccgaca cggtgaacgc cgaggcgaac cccgagccgc cggagattac
    26401 gcggacgtgg gcgatataca gcccacccgt atgcgagaag gtgccgacgt tgtaggagtt
    26461 gacaccgacc gacttggcga actgtccggc ctgggattga tcgacgatgt acatcgcgcc
    26521 cgaaggggcg ggatcacagg cgaatccgaa ggactggtac gcttcgcaga tcttgctctt
    26581 cggaatattc tggaccttgt agcgcgcgag ctgctggacg cggtcacgag cgagatccat
    26641 gacggcgatg ctggccgcgg agtctccggc ggcggcaccg gggagcggca acagcagaag
    26701 tttctcggtc ggctgggtga tggcgagctg agccgacgcg acggatgcgg ctcccatcac
    26761 cattacggcc accagacgga acactctcac gcaggtcctc cgtttagggt cgtgctgctc
    26821 actacaccga tgggcgaaga gggattcgaa cccccgacat cctgcgtgta aggcaggcgc
    26881 tctgaaccag ctgagctatt cgcccgtcca acaaccctta gagggcgagc cctaccggaa
    26941 tgaataccag atagcccagc accaacagca ccggtgccag ggtggtcgat ccggcattca
    27001 aagacacata ccccgcggcg aggcagacga gcgccgcccc gaaggcggcg tagttccggg
    27061 ggccgaaatg caggcgttcg cgagattgcg tatcaggcat tggcggctcg catactaccc
    27121 cgcaatcgct tttccgtcaa ggatttggct cgtctggggg tatgacccac ccctcgtcca
    27181 aggccgcccg ccggggagcg tcccgcttcc ggcggcgcca cacgagcccc acgagcaaca
    27241 atccgatgac accccagaac agcgtcgcgg cgccgagccc ggcgagccac ccataccgcc
    27301 ggtgcacatc ctgcgcccag agctcttcga agtggtcgag ggtgacgtga tacgtcgatc
    27361 gcatcgcatc atcgaaggtc ggctgggtgg cgagggcgtc gatgaaggga tggagtccgg
    27421 ccggaccgcc ccaccgctgg agcatgccca ccgcggtcgt tgcgagcgcg tacgcccccg
    27481 ccgcttcgcc ggcgcgtcca cgcaacgccc gatccacact gtcgagctcg agggccggcc
    27541 cccccagcag atgccagtcg agctcaaaag cctgcgtgcg gttccactcc cccgccgcca
    27601 cggccgcgta cccttcttcg aaccagaggg gcagcgagtg ccggacccgc cggcgcaggg
    27661 ctacgtgcgc cagctcgtgc cggagggaca ctcggagatc ggacggcgag cccaccgacc
    27721 agaggacgat tgcgcccgaa cccgggaagg ccgcgccctc actccagctc ggcagccgtc
    27781 cccgcgtgag cgagtcgtag atcgcacgat tgggagcgag gaagatccgg aggggccggt
    27841 ccggctgcgg gtcgatgccc ggaaatggat tcgggcggtc agcttcgtcg gccagggcag
    27901 ccgcaagatt tccctgcggt ggccaccaca ccacagtgac gttgccgacg acggtcacct
    27961 gcggcccctg aagcaggatg gccgacaggg ccaggacggc cgggagcgct agggtcgcgg
    28021 gggctcctcg ccacggccca ccgcctcgag catttcctgg cagcgcttgc cccagggatt
    28081 gaacttgttg gccttgaacc cgtcctgcca ccactgcttg gcctgcgcct gatcccccgc
    28141 aaaccagaac gcgcgcccca cttcgtagta cgcctcgatc agattgggac cgagcttcag
    28201 ggtcttctgg aaaaacgtga ccgcgtcctc gtacatctcc cgctggaggt acacgaggcc
    28261 gaggtagaaa tgggcgtaga gtgtggcctt cttgtcgttg tcgaggcgga tggctttcga
    28321 gagatgctcg atcgcctcac cgaagatctt cttcttgaga cagatatatc cgacgttgat
    28381 ccgggccagt gaattcgccg gctccttcat cagaaccttc tggaacgtgg cgagcgcgtc
    28441 atcgtagtcc tcgtgcagca tctgcgccca gccgagcatc gactccgcct gcgtctcgcc
    28501 gggtgacaga gccagcgcct tctgcaacgc ctgaatcgcg cccgcgtggt cgcccagcga
    28561 gatcagactc cagcccttct cgatgaacgt cgacgccccg atgtggtcag catgcaccgc
    28621 gggccgggtc cccgtgaact ccggagccga cgcttccgcc gccgaggccg acacctgttt
    28681 gtacttctcc accagctgac ggatgtcctc tttcaactgg cccaggtcgc ccaacaggcc
    28741 atccacgtgc ttgaaaaacg cgatgatttc gcgcttcacc gcctcctgct cggcggtggc
    28801 tttggcggtg tcgagcttgc gggcgatggc gtcgtactgc gcccgcagga ggaccaccgg
    28861 cgagtcggcc atcaggcgct ggccttttcg gtcaaggcgg cttccagggc gactttctcc
    28921 ttcgaagcga gcagttgcgc cgtgttgagc acaatgatcg tccggcctcc ggtcaccacc
    28981 aggccggtga cgtacgcggc cgccagtccc ttcacgattc tcgggggcgg cgtcacttct
    29041 tcggcgccca cctgcaacac ctcggtgacc acatcgacca cgagaccgat cttcccgcca
    29101 tcgtactcca ggatcaccgt ccgggtgtcg tcgcgcacgg gtgccgggac cccgagccgc
    29161 ttgcggaggt cgatcacggg gacggcggcc ccttcatagc gcaacacgcc ttcgaggaac
    29221 tccggcgcct tcgggagggg cgccggacgc tcataccgga ggatccgctc cacctggaag
    29281 atgtggaagg cgaagtcctg gccggccacc cggaacgtca ccagctggac atcatcgccc
    29341 gcgctcattg gatccgttcc cccagtgcct cgaggtcgag tatcggaacc aggccgccgt
    29401 cccggcggcc catgccggac gcccacggca aggattcccc gggcggcacc ggcagaggcg
    29461 cctcccggac cacttcgtcg gcgtcatcca cttcgaactc caccagccgc ccgccgatgg
    29521 tgacgagcac catcgcgcca ccgcgttcga cgggaggcgt ctgattcttg aggagcgccc
    29581 ccaggtgaat caacggcacc agccgcccgc gcaccggggt gagaccgcgc accgccggca
    29641 gcgcccgggg cacgtcgagt acttcgccca ggtctcccac ttcgagcacc ggcccgagcg
    29701 gaagcccata gggccgccca ccgacgcgga ccatgacgta tccgacgccg cgtcccttca
    29761 cgcgcgcgcg gcccggcgga tcagctcacc ggacacccgg tcggcgattt ccggcaaggg
    29821 cagcaccgcg tcgaccccgc ccgcttgcac cgccgcgccc ggcatgccgc cgatcacgca
    29881 agtctccttg ctctgagcga tccccatgcc gccggcatca tgcacggccc gcaacccatc
    29941 ggcgccgtcc cggcccatcc cggtgagcac cactcccagc gccttgtttc caaatgtctt
    30001 cgcgaccgaa cgaaacaacg gatccgccgc gggacgcacg ccccagatcg gcgccgactg
    30061 atcgagcgcg atgatcggtc cgtcgggccc cggctccacc cgcatatgat aatcccccgg
    30121 cgcgacgtat gccgtgtcgg cgacgagggg cgctccctca tccgcctcta ccacgcgaat
    30181 ggcgctcatc gagtcgagcc gctcggccag gctccgcgtg aagcgcgcag gcatgtgctg
    30241 caccaccagc acacacgcca tccggcccgg atgcaggtgc gggatgagtt ccgtcagggc
    30301 tcgcgggcca ccggtcgacg cggcgatggc gacggccagc ttcgccctgc ccttgagccc
    30361 catctccggc gcgtgcgcca ccggcttggg agggcgcgcc aggacggtca cccgcgagag
    30421 gtcggcggcg agcgcccggc ggaccgcgtc gacgaggagc ggcccgagcg cctcgagcga
    30481 ttcccggttc cgttcgtccg gcttcgccac gatctccacc gccccgagct cgagggcccg
    30541 gatggccgcg gccgtgccgg ggcccgcgtg cgcgctcacg acgacgatgg gacgtgggga
    30601 ctcggacatg acgtagccga tggcgccgag gccgtcgagc tcgggcattt cgaggtccat
    30661 cgtgacgacg tcgggctgaa actggtggac tttgcgaacg gcgtcgaagc cgtctcgcgc
    30721 cgtgccgacg acccggaagg ggccgttgtc ccgggacccg cccccgagca catccacgag
    30781 cacacggcgc atcagcgcgc tgtcatccac cacgaggacc gtcggcctgg tgtcagaggg
    30841 tggtgacatc ggcgcccacg gtccggattt cgacactgcc atcgctcacg tgaaagcgca
    30901 ccgagcggcc ctgcccgccc ccaaccgcct ccgcgacgat ggggatggcc gcgtcccgga
    30961 gcgcattccg tgacgccacg acgttccgct cccccatctg aatcgtcccc ggggtcatca
    31021 gggccccgaa catgctcgcg ccgccggcca gcttcgcgac cagccggcgc gggtcggctc
    31081 ccaactgggt catccgctgg accagcagtg gtaccgcggt ctcgggaaac ttggcggggt
    31141 tggtcgtgtc ccgggcgagg gagcgggacg gcagcagcac gtgggccatg cccccgatct
    31201 tcgtctcctt gtcgtacagc atgatcgcga cgcaggaacc gagcccgaga gtcaggagat
    31261 cggacgcccc ctggtccgcg gcccagtcgg ccacggtgac caccgtctcc ttggtcttcg
    31321 gggccgaccg ggtcagaggg gccgccggta gatccgctcg cgcggatctt cgagctcgag
    31381 gagctgccgg ctgtcgccga acagggtctc gaccttcccc aggaccagca gtccgccggg
    31441 ccggagcgac tcggcaaagc ggacgaacag tttctcctgg ctcgggcggt cgaaatagat
    31501 caccacgttc cggcagaaaa tgagatcgta gggcgggcgt ggggcccgct ctttcagaag
    31561 atcgttgcgc gcggcatgaa ccaggttctt gatgaggggg accggttgcc ggggggcggg
    31621 tttggtgaag tagcggtcgg ccatcgcctt cggcatctcg acgaacgcgg attccggata
    31681 ctgcgcggcg gccgtccgct cgagggaagc ccggtcgatg tccgaggcgt cgacgcgggt
    31741 ccgccccagc tgtgcctcca tccccgctac ccgggcgata tcggcgagca gtgccgcgat
    31801 cgtgtagggc tcctccccgg acgcgcaccc cgcggaccac gccgcgtact gcccttcgcg
    31861 ttccttccat gcagccgcga ccatcgggcg caccgcatcc caggtttcgg cattccggta
    31921 gaacttggtg acgttgatgg tgagtgcgtc gaggaggtgc tcgtactctt tcgcgtcctg
    31981 gatcaagcgg gtcgcatacg cgtcgtaggt atgcacgccg caggcccgca tgcgcaccgc
    32041 gatccgccgc ttcaggcact tgtccttgta cgcttcgcag gagaacccgc gggctgtggc
    32101 gatctgctga gtgagcgcgc gaaaggcgcg ctcatcctca tccggggtca cttgagcgcg
    32161 ctcaaggtgt ccaggttgga tttgaccagc tcgtgcgccg gattgagctc gagcgccttc
    32221 ttccacatct gcgacgccag ctcccggtca tttcgcttgt aggcgatgtt ccccagcttg
    32281 aagtagacgt cgtcgccgat ctccggcgaa agctcgatgg tccgctggta cgcctcccac
    32341 gcttcgtcgt agtgcgcggc ccggtactcc agatccccaa ggttcttcga gagctgggga
    32401 tgcgacggct cgtcccggag cgcgctccgc accaactcct cggcgcccgg caagtcgccc
    32461 gccagctcgc gcaggaccgc ccggttgttg cgcaacaccg cattttccgg gaacgccgcc
    32521 accgcggctt ccgcgagctc atcggcctgt tcgaactctc ccgacgcggc ggccgccagg
    32581 gtgcgcgccc agtaccacac ccccggaacc ttctcaccga agagctcctg ggcccggtcg
    32641 agccggccgg cggcgctttc gtagtcgccc cgcttgagcg ccaccactcc ccatccgagg
    32701 gcgatccggg cgtcgttccg gttccgcgtc gcggcctccg cgtacgcgct ttcggcgtcc
    32761 tcgaggcggc cgaggtgctc atacgccagt ccgaggttgt ggagtaccgc gggccgcgat
    32821 ccgccctttt ccgccgcgag ccggaacgct tccatcgcct cgcgccaccg cgcctgccgc
    32881 aaggcgacca gtccggtgaa gaacagggcg ttcgcatcct cgccgcggag ctcggtgacg
    32941 cggcggaact cccgcgccgc ctcctccagc atgccggtct tgtagaacgc gacgccgagg
    33001 ttgcggtgct cctccacgcg ggcgtccgtc accttcgcat cttcggtctg cgtcgcggtc
    33061 gtgcgtcccg cccggtgcag gaagcccgcc gtcaggagcc cgtagagggc cttcccgacc
    33121 tcgaattcgc cgaggccggt gtcttcgatg agggtgtgaa tgtcccgttc gccgtcgatc
    33181 agcgggagca aacgctcctg ggtcggcgtg aggctgactt cgctgatctc gagatggtcg
    33241 cggtccacga tgaagatcac gtcgaaggac gggatcttct tctcgatcag gctccactcg
    33301 tccacccgcc gggcgccctc gagcaggagc gactcgggat tgatggcgac caggaagtcc
    33361 tggcgctcgg gacggacgtc ggcctcgaag ttgaacgttc cctgggtcca ggtgaacagg
    33421 aagtacaccg actcctcgat ctgcacgcgc atgtagcgct cgaggtcacc ctgggtgatg
    33481 gccccctgct ccaccaggat ggcgcccagc ttcttgtccc ggttcttcga ttggccctgg
    33541 agatgcacgc catcttccag ctgctgctgc gtgatcttct cgtgcttcac caggatgtcg
    33601 cccagccggt cacggcggtt gacgatgctg gcgtagcaga tacggccctt ctcgaaaaag
    33661 atgtagccga agttctggcg gtcggcgatc gacaggcacc ccgtcttctg gccgagggcc
    33721 agcagctgga gcacatcggg aagtgacgct tccttgagtg agcccttgat cgccatctag
    33781 cgcgcgtcct cgatcatccg gtccgccggc accggccagt cccacaccat cttctcgcgg
    33841 ctgcggacgc cgcccgcgct cgataccacg atccccgagg tccggaatcc ggacgtacgc
    33901 cgccggggtg ccacttcccc tccgagtacc gcgcgcgcca ggccggccgt catcggctgc
    33961 gactgggcgt cggcggcgtc cttcacccgc tgcacctccg ccatcatcgc gcggatcgag
    34021 gtcgccgggc ggtcggcgag ataatcgagg agcgagcctt cgaccttgcc caggctccgc
    34081 tccagcacgg cacggcggac cacccggtcg ggttgcgcga gcgtgaccac gagaccgccc
    34141 gccaggcggg attgaatccg ttcttcgagc ccttccagcg tgttcggggc cgccggcgcc
    34201 gtgaagacga gctgccggtc cttgtcctgg agcacgttga aaagattgaa gagctcttcc
    34261 tgggtccggt ccttccccgc caggaggtgc acgtcgtcga ggaggagcgc ggtcgcgcga
    34321 cggtaccggg cgcgccacca gtccacccga tccccctcga tcgccgcgat cacttcgtcg
    34381 atgaacgtct gggtcgacag gcaggccacg acggcccgcg ccttcgtggc gagctcgagg
    34441 ccgatggcgt tgagcagatg cgttttgccg agcccgctgc cgcccaccag caccagcggg
    34501 ttgaacttct ttcccgggct ctggacgacg tcgtgaatcg ccgccagctc cttctcgttc
    34561 gaagggccgg ccacgaaggt gtcgaaggga aatgcggccg agggagccgg tggcggcgcg
    34621 gcgccttcgc gcacgcggac ggcgaccttc tccgcctcat ccagacgctc ggggtcgcgg
    34681 aagacgctgt cgccggccgc ctgcggctcg agcatctgca cttcggccgc cagaaccttg
    34741 agccgctcca cgtccttgac gaatcccgag atgacctggt cgaccgcgcc gggggtttcc
    34801 tgcgcgagca gcgcttcgag ccgtttggtt cgaaagccct ctccctccca gcgcaggatg
    34861 gcctcgctca cacgggccct ccacgcctcg accgcgcgtc ccacggtagc cgtgacgtcc
    34921 gagaggaagt tcgagaactc gtcgggagcg ggagcggacg cggcggcagg ctgggccgcc
    34981 gcggcatcct ccgcggaacc gatgcccagc acctggcgcg ccgcggcctc ggtgatcggc
    35041 gtgtcgttca cggcctggaa ggcgatcagg cggttcaggg cccccatcag ctcgcggacg
    35101 ttcgcgaact ccacgcccgc cacggtctcg agcacgcccg actggaagcc ggtgccacgc
    35161 tcctcagcct tctttcggag gatggcgacg cgggtctcgt agtcggggat gcccatgtcg
    35221 accacgaggc cgccggagaa gcgcgagatc agacgctcgt ccaggtccgc gatctccccg
    35281 gggggccggt cggacgcgag gaccacctga tgccccgcgg cctgcaatcc ctcggtcagc
    35341 cgcaggagct cggcctgcat ctccttccgg ttcgtgagga actgcacgtc gtcgaccagc
    35401 agcatgtcca cggcctgaaa acgccgccgg aacgtgtccc cctgccccgc cgcgatcgcc
    35461 gcgtgaaacg cttcgacgta ctcgtccagc gtcaggtatt ccaccgagag gttgggcgcc
    35521 accttcttgg ccccgtgtcc gatcgccatc agcaggtgcg tcttgcccag gcccgacccg
    35581 gaatagatga aaagcgggtt gtacgcggat cccggattct cggcgacggt gcggccggcg
    35641 gtgaccgcca gccggttcgc cgcgcccacc acgtaggagt cgaaagagaa tttcggattg
    35701 agggccgagg tcacgcgggc gccccctgtg ccagcttctt caagaccagc tccgaaatgc
    35761 ccttcaggct ttcgaacacg cctgtcccat ggagggcgtc cgccgagaag ctggggaccg
    35821 cgcggaagtt cagcgcgtca tccatctcgg ccggcgtgag aatcagctcc ttgggaaggt
    35881 cctgcttgtt gtactggagc acgagcggca tggcgcggac gtccacgccc tggtcgagca
    35941 ggttggcgtg caggttctgg aggctttcga ggttctcgtc gagctgacgg ccctggctgt
    36001 cggccacgaa cacgaccccg tccgccccct gcagcacgag gcggcgggtg gcgttgtaat
    36061 acacctgccc cggcaccgtg tagagctgga agcgggtggt gaaccccgaa atgctcccca
    36121 gatcgatggg caggaaatcg aagaacaggg tgcggtcggt ctgggtcgcg agcgacacca
    36181 tgcgcccgcg gcgcgcctcc ggcacccgcc cgtagatgta gtgcaggttc gacgtctttc
    36241 cggaccgtcc cggtccgtag tagacgattt tgcaggtgat ctcgcgggtc gtgaaattga
    36301 cgagcgacac gcgctatctc ccgaaaagcg agttgaggct gttcgtcaat tcacgctcga
    36361 agtcggcggc cagtacctgt ttctgctcgg agcccttggg gctcgccccc cgcaaatcgg
    36421 tggcgagctg gtcgaagaat agctgaacga ggcccaggga cgtggcggac agatcgtaaa
    36481 ccgcgagcac caccacgggc ccccggtcgg tcgacaccga cgagaggaag attccatgct
    36541 tctctccctg gtgggacagg cccgcgaagg gcgcctcgcc gagctgccgg gccatctccg
    36601 tcgtcgaggc gaagatggcg gctcccagcg ccgcggccga catcacgtcc acggcccggg
    36661 cgaacccgga ctgggccagg acctgtcccg ccggcgtcat caggagcacc agccgaacgc
    36721 cggcctcctg cacgaaggtc gtgaggggtt cgagggtcca ggcttcgacc gcgcgcgccg
    36781 tcatgccacc ccctccgcgg ccttcagcaa ctcgagacgg accaggccgg cgttgacgcc
    36841 cggctcggcg accgcgacga tgagcatgcc ggaccgcgcg ggcaccacga ggagcgcgcc
    36901 gcgttccgcc tgcaggtgcc agaagacatc gctccccaca cctcccgctt ccatggcgcg
    36961 tcccagccgg gtggccagac tcgccgccag cgcggccacc gcggcgccct tgatgccctc
    37021 catcaggctc tccgccacca ccaggccgtc ggccccggcg accaccatcg caccccg
//
LOCUS       DASUBP010000280         3865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_650336, whole genome shotgun sequence.
ACCESSION   DASUBP010000280 DASUBP010000000
VERSION     DASUBP010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3865
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_650336"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(174..668)
                     /locus_tag="VFU75_10685"
     CDS             complement(174..668)
                     /locus_tag="VFU75_10685"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU5467347.1"
                     /translation="MIGLALLLLAAAPVVRGQDPDDVGIPVGSTPAAPKIEDLSRNQV
                     DLAKYVGHGPVLLEFWAQWCPICKELEPKMAAAHAKFGKQVTFIAVSVAVNETPASIQ
                     RHLTEHPMPYQVLWDSGGNATRAFQAVATSYVVILNAKGVVTYTGTGSDQDIEAAVEK
                     TIKS"
     gene            complement(710..1432)
                     /locus_tag="VFU75_10690"
     CDS             complement(710..1432)
                     /locus_tag="VFU75_10690"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HEU5467348.1"
                     /translation="MLHLLALAGTPLSASLRDHPLAALATLFGLGVLYSLSPCIWPMI
                     PITAGILGGSANAKTSRARTALLSVTYALGLALVYAVLGLVAGLTGTLFGTVSASPWA
                     SLITGNLLLLFGLVMLDVIPVRVPQRLMAWAGGQSGGSYPAVFLMGASSGIVAAPCGA
                     PAFAVVLTWVAATRSGVLGFFYLFAFAFGMTAVLVAVGLFSGILGSLPKAGPWMAWAK
                     RVSAVVVLVMAEYYFIQAGKVW"
     gene            1648..3066
                     /locus_tag="VFU75_10695"
     CDS             1648..3066
                     /locus_tag="VFU75_10695"
                     /inference="COORDINATES: protein motif:HMM:NF014385.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HEU5467349.1"
                     /translation="MSAIGTIVFLVAGCAVGTSPSPGTPWTPPASAQRAPAPAPTPVP
                     PDLEARVRTLRLPDVLDIALRNNTVTREAWYNARAALYTYDATRGQYFPSLTLDGSIT
                     KLKTSPSQGRSAVTQTVYGPALNLSWLLFDFGGRSGSISAARDQLLAADWTHNAAIQD
                     VVLTVEATYFNYIGTRALLESDRITLAEADSNLAAAQGRHDVGLATIADVLQAKTARS
                     QAELALETTSGALQTARGALAVSMGFPANLPYDIDSVPLPTPPPGVAESVDSLISRAL
                     VARPDLAAARAQAQAAQARVSIARGAALPGLSVTGSAGDTYFANRPPVPGSSYSISLG
                     IEIPLFAGGAHVYGIKSAQAVADAARASAEGQAQLVISQVFTSFYGLQTATQRVRTTD
                     DLLASATQSEQVALGRYKAGAGSVLDLLTAQSALADARAQQVTAHFGWYASLAQLAHD
                     VGVLGLDGSSPIRLNSDTAGSH"
     gene            3066..>3865
                     /locus_tag="VFU75_10700"
     CDS             3066..>3865
                     /locus_tag="VFU75_10700"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEU5467350.1"
                     /translation="MTRTFRFAFAGPLVLVAACSQGHARGAPTIPVSAVQVTTKDVPY
                     ELTATGTVEPRQTAQVEAQVTGIVTRVAFHEGDEVAAGQLLFQIDPRPFQAALAQAVA
                     TMHRDSAQAMQADRDADRYATLVKQDYVTQEDYDTKRSTAAAADAQVRADSANVTAAR
                     LNLEYASVRAPIGGRTGGLLVKEGNLARANNVTPLVIINQIRPILVRFAVPQANLPQI
                     QRFKGNDLQIRVNPGAGDTASDEVGRLTFMDNAVDTSTGTVLLKGEFA"
BASE COUNT          677 a         1373 c         1209 g          606 t
ORIGIN      
        1 tgttcgtgat cgacgggagc ggcaagatca cccacgtgat cgcaccgttc cacgtgctcg
       61 atccggccga ctacaacacg atgaaggctg cggtagactc ggtaacgaag tagcccgaca
      121 agaaggcgtg gggaggtggg agtgtgagac cacttcccca cgcatttttt ggcctagctc
      181 ttgatcgtct tctctaccgc cgcttcgata tcctgatcgg agccggtgcc ggtgtaggtc
      241 acgactccct tcgcgttcag gatcaccaca taggacgtcg ccaccgcctg aaaggccctc
      301 gtcgcgttcc cgccggagtc ccacagcacc tgatagggca tcggatgttc ggtgagatgc
      361 cgctgaatcg acgccggtgt ctcgttcacg gccacgctca cggcgatgaa cgtcacctgc
      421 ttgccgaact tggcgtgcgc cgccgccatc ttcggctcga gctccttgca gatgggacac
      481 cattgcgccc agaactcgag caacacgggt ccatggccaa catacttggc caggtccacc
      541 tggttgcgag agagatcttc tatcttcggc gccgcggggg tggaacccac cggaatcccg
      601 acatcatcgg ggtcctgccc ccgcaccacc ggcgccgccg ccaggagcaa cagcgcgagc
      661 ccgatcacta catttcccgc ttcccgtttc acgtttcccg tcctttgact taccagactt
      721 tcccagcctg aatgaagtaa tactccgcca tcaccagcac gaccaccgcc gagacccgtt
      781 tcgcccaggc catccacgga cctgccttcg gaagggatcc gagaatcccc gagaacaatc
      841 cgaccgcgac cagcaccgcg gtcatcccga acgcgaaagc gaacaaatag aagaacccca
      901 gaacaccgct ccgggttgca gccacccagg tgagcaccac cgcgaacgcc ggtgctccgc
      961 agggagccgc aacgatcccc gacgatgcgc ccatcagaaa gaccgcgggg taggagccgc
     1021 cactctgccc gccggcccac gccatgagac gttgcgggac ccgcaccgga atgacgtcga
     1081 gcatgaccag cccgaacagc agcagcaggt tgcccgtgat cagggaggcc cagggagacg
     1141 cggacaccgt tccaaacagg gtgccggtaa gccccgccac caaccccagg acggcgtaaa
     1201 ccagggcgag tccgagcgcg taggtcacgg acaaaagggc ggtgcgggca cgactcgtct
     1261 tggcgttcgc cgacccgccg aggatgccgg ccgtgatggg gatcatcggc cagatgcagg
     1321 ggctcagact gtagagcaca cccaggccga acagcgtggc aagcgccgcc agcgggtggt
     1381 ctcgcaaact ggctgacagg ggtgtaccgg cgagcgccag gagatgaagc acggagggat
     1441 tatacatcca cgggtgcggg gagccgtcac ggtacagtca ggcccgaaaa ggcgcactcc
     1501 ggttagtttc tcggcatgac cgcgtcacag ctttcgcgcc ccgtcgtgcg tattcggggg
     1561 gaggtcagtt ccaatccccc tttcacaacc agggtgattc tacggatgca ggttgctacg
     1621 cgccgcgctt caagcacttg gctgcccttg agcgccatcg ggaccatcgt cttcctggtg
     1681 gcgggatgcg ccgtgggcac gtcaccctcg ccgggcacac cgtggacgcc gccggccagc
     1741 gcgcagcggg ctccagcgcc ggctcccacc cccgtgccgc ccgacctcga ggcgcgcgtg
     1801 cggactctcc ggctccccga tgtgctggac atcgcgcttc gaaacaacac cgtgacgcgt
     1861 gaggcctggt acaacgcccg ggccgccctc tatacgtacg atgccacccg cggtcagtac
     1921 ttcccgtcgc tgacgctgga cgggagcatc accaagctca agacctcccc ctcgcaaggg
     1981 cgctcggcgg tcactcagac cgtgtatggc ccggcgctca acctgagctg gttgctcttc
     2041 gatttcggag gccgctccgg gtcgatcagt gcggcgcggg accagttgct ggccgcggac
     2101 tggacccaca acgccgcgat ccaggatgtc gtgctgacgg tcgaggccac gtacttcaac
     2161 tatatcggca cccgggccct gctcgagtcc gaccgcatca cgctggccga ggccgattcg
     2221 aacctcgcgg cggcgcaagg acgccacgat gtgggactcg ccaccatcgc cgacgtcctg
     2281 caggccaaga ccgcccgctc gcaggccgag ctcgcgctcg aaacgacctc cggcgcgctg
     2341 cagacggcgc gcggggcgct ggcggtttcc atggggttcc ccgccaacct gccttacgac
     2401 atcgactcgg tccctctgcc cacgccaccc cccggcgttg ccgagagcgt ggactcgttg
     2461 atcagtcgtg cgctggtcgc gcgccccgac ctggccgcgg cccgcgctca ggcccaggcc
     2521 gcgcaggccc gggtgagcat cgcgcgcggt gccgctctgc ccggcctttc cgtgaccggt
     2581 tccgcgggtg atacctactt cgccaaccgg ccaccggtgc ccggctcgag ttactcgatc
     2641 tccctcggca tcgagatccc gctcttcgcg ggaggtgcgc acgtctacgg catcaagtcc
     2701 gcgcaggccg tggccgacgc cgcgcgcgcc agtgcggagg gtcaggccca gctcgtgatc
     2761 tcgcaggtct tcacctcgtt ctacggattg cagaccgcga cgcagcgggt ccggaccacc
     2821 gacgacctgc tggcgagcgc gacccagtcc gagcaagtgg ccctcggccg ctacaaggcg
     2881 ggcgccggga gcgtgctgga cctcctcacg gcccagagtg ccctggccga cgcgcgggct
     2941 cagcaggtta ccgcccactt cggctggtac gcttccctcg cgcagctcgc tcacgacgtc
     3001 ggcgtcttgg gactcgacgg gtcgagtccc atccgcctca actccgacac ggccggttcg
     3061 cattgatgac gcgcacgttc cgtttcgcct tcgccggtcc cctggttctc gtcgccgcct
     3121 gctcccaagg gcacgcccgc ggggcgccga cgattccggt gtctgccgtc caggtgacca
     3181 ccaaggacgt cccttacgag ctcaccgcca cgggaaccgt ggagccgcgg cagaccgcgc
     3241 aggtggaagc gcaggtcacg ggcatcgtga ctcgcgtggc tttccacgaa ggagacgaag
     3301 tcgcggcggg tcagctgctc ttccagatcg atccccggcc cttccaggcc gccctcgcgc
     3361 aggccgtcgc cacgatgcac cgcgactcgg cgcaggccat gcaggcggac cgtgacgccg
     3421 accggtacgc cactctggtg aaacaggact atgtgaccca ggaggattac gacaccaaac
     3481 ggtcgacggc agctgccgcc gacgcccagg tccgggcgga ttccgcgaac gtcaccgcgg
     3541 cccggctcaa cctcgagtac gcgtcggtcc gggcgccgat cgggggcagg accggcggct
     3601 tgctggtgaa ggaagggaat ctggcacggg cgaacaacgt gacgcccctc gtgatcatca
     3661 atcagatccg gcccattctc gtgcggttcg cggtgccgca ggccaacctg ccgcagattc
     3721 agcgcttcaa gggcaatgac ctgcagatcc gcgtgaatcc cggtgccggc gacacggcct
     3781 ccgatgaggt cgggcgcctg acgttcatgg acaacgccgt cgacaccagt acgggtaccg
     3841 tgctgctgaa gggtgaattc gccaa
//
LOCUS       DASUBP010000281        11029 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_814271, whole genome shotgun sequence.
ACCESSION   DASUBP010000281 DASUBP010000000
VERSION     DASUBP010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 11029)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11029)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11029
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_814271"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="VFU75_10705"
     CDS             complement(<1..581)
                     /locus_tag="VFU75_10705"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5467351.1"
                     /translation="MTDLFDDLRNGLAGRYELDRQIGEGGMAMVYLATDLKHDRQVAV
                     KVLRPEVGAVVGADRFLREIRVTAQLQHPHILPLFDSGAIGSSLYYVMPLVQGGSLRG
                     RLEREGPLPVEETVNMIRALASALDYAHRHGVLHRDIKPENILLHDGQPMLADFGIAL
                     AVASAGGPQRLTGTGISMGTPSYMSPEQVSGDR"
     gene            complement(594..1781)
                     /locus_tag="VFU75_10710"
     CDS             complement(594..1781)
                     /locus_tag="VFU75_10710"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEU5467352.1"
                     /translation="MDRRSFVATTAATFAAAKVHVHTPGDPLGVRDDFPITAQRTYLN
                     SAYIAPVPRAVVQAGAEFLENKSKRPLEVGELLGGDGKLRSQFAQLVNASPDEVGLLF
                     STAEGENIIAQGMELRAGDNVVIDDLHYPTEFVLYRALEASKGIELRIARNRDGAVSV
                     TDFEPLVDDKTRIVSVAWVSHLNGFRHDLRPLADLAHAHGAVLYADVIQGLGTIELDV
                     QAAGVDAVCAGSYKWMLAGFGVAPFYIRKEVLERLKIDRYGEFQVEKELPDYHFELDQ
                     TARKFDYCSRAFGPVKELSAALTYLQGVGVGRIEAHTVALGQRLHDGLTRQGHKLFTP
                     PGNRSPIVTMFTPKPMADVRKAFQGASVDVTVRDGQIRIAPALFNTEEEIDRCLEVTK
                     GLV"
     gene            complement(1781..3385)
                     /locus_tag="VFU75_10715"
     CDS             complement(1781..3385)
                     /locus_tag="VFU75_10715"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEU5467353.1"
                     /translation="MSVPAAAQRPPLDTIYTHADTLRGSNGPARAWWDASFYDLHVRV
                     NPADSSVRGWNGITYRVLKPAQEMQIDLQIPLAIDSIVQDRKKLTSRRDGDAFFVTLK
                     AAQRTGDAKTITVYYHGQPGRDRPRGGAGFIWAKDSLGNPWLATSDEGIGASIWWPVK
                     DFPADEPDSQRIAITVPDPMIDVSNGRLRSTTHNADHTTTYEWFVDDPINMYDVAVNA
                     GQYAHFSDTLQGESGPLSLDFWSMAYHTDTAKGQWKQATPMIQCFEHWYGPYPWYKDG
                     YKLIEQSYLGMEHQSGIAYGNKFLRGYLGRDLSGTGLGLSWDYIVIHESAHEWWGNNL
                     SASDVADQWIHESFATYSEGIYTECQKGKTAGEAYLIGLRRSISNRYPIIGPYGIGRW
                     YHNDLYMKGANVLNTLRQVVDDDARWRKILRGLQKTFWHQTVSSHQVETYISQQAGVD
                     LSKFFDQYLRTTKVPEFDYKLDGTTLSYRWSNVVTGFDMPIRVEVPGSGTHLLKPTEE
                     WQTLTIAPAGAAPTVDENYYVTVKGQ"
     gene            3530..4279
                     /locus_tag="VFU75_10720"
     CDS             3530..4279
                     /locus_tag="VFU75_10720"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEU5467354.1"
                     /translation="MTASLPVLSLIIPVRNGAEFLPHTLGELRAFMMREPCAEVVLVD
                     DGSEPRTAALLAAFACDQPRVTLLVHEVNQGKGAAVARGMLAARGRVRVFTDADLAYP
                     LDQLPAVCAAIDQGSEVAIACRVLGESRYLMSPTFFHYLYTRHLLSRAYNAVVRWTLL
                     PGILDSQAGLKAFSARAAEVIFPRMTIRGFGFDVEALLIARRHGLETVQVPVHFRYDS
                     EPTTVRLAQDGGTLLSDLARIRWNDLRGRYR"
     gene            4287..5090
                     /locus_tag="VFU75_10725"
     CDS             4287..5090
                     /locus_tag="VFU75_10725"
                     /inference="COORDINATES: protein motif:HMM:NF016668.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChbG/HpnK family deacetylase"
                     /protein_id="HEU5467355.1"
                     /translation="MVINADDFGISDGVTRGIVEAHRGGVVTAASMFANAPGFAAAVQ
                     AARELPRLDLGLHFNLTAGVPVAPPAQVSSLCGAGGGFLPLGALIRRALAGRVDPDDV
                     ARECRAQLDRLRTNGIVVSHLDGHRHAHVLPSVWPGVAAVARCEGLPVRKPVEPLRRG
                     RPATKLALGIAYGFVTRHAGEGPPSPHFRGIGLLGAWHFAEALIELIDHLPPGVTEVM
                     VHPGYVDADLLRWDSYGQERERELVALRSPQVLTRLTRGDPQLVRFVDL"
     gene            complement(5047..6168)
                     /locus_tag="VFU75_10730"
     CDS             complement(5047..6168)
                     /locus_tag="VFU75_10730"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HEU5467356.1"
                     /translation="MKWIWGLYAVSVLLVTLQKGLIHPGNFLLFRCTFDRLIHGQGLY
                     LNTPDCFGYLYSPAFALLFAPFALLPPLLGLFCWNAINAAAPAWAIARLLDPRRAQLV
                     LVFIYLDVVRSMQNSQSNALVAGLLILTFLACERESIVGAALMTGAGALIKIFPAAGG
                     VFALVSPQPAGVRLKRIAIFVVAGALLALTPLLVTAPSMLVAQYHAWLTTTTTTSVLR
                     GESVLGILGTWFGYAGPNWPLQLAGLVTLLAPVILLRRAARHPEFRRLFLCSLLVFVV
                     IFNHQAESPSYVIATAGIGIWAVTFGERWRVVLAILTLVLVSLVATSLVPWHYRHDVL
                     RAYSVQAVPCLLCWIGIQADLWRMRATGPRTAPAAGLPG"
     gene            6343..8169
                     /locus_tag="VFU75_10735"
     CDS             6343..8169
                     /locus_tag="VFU75_10735"
                     /inference="COORDINATES: protein motif:HMM:NF020037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEU5467357.1"
                     /translation="MSETPLPTPTAPLTPDAQDRLLHLFTQQIPARACATDRDLRVVW
                     DNGSAFPDSPSAVGKTVSELFAASPDRERVVEACVRALKGEGTTLEINDGTSAAHLRL
                     VPFHDWDGEIVGVVGVAFDTTSLVRSKEDVESGQLLLRQVLDTLPVGVFVMNRSGDIL
                     LDNPASRNIWGGSIRSGPERWARSIAVWHNSGKPIAAEEWASRRAIARGDVSRDELID
                     IQTHDGEGHKTIENYAAPIRGPKGDITGAVVVNQDVTERVRAVEALRKTERLLVDAER
                     LGQTGGWEQDLVTGEIVNTDANARLFFGDDRSKGARLEDYADAVHPDDRAWVMARRER
                     MLAGTGSTEIEYRVIWPDGTVHWVFGRATVVRDESGKPVRVYGTNADVTGRKLAEEEL
                     GRRARQLEALSRRLIEAQEAERRAVARELHDDFGQVLTALKLNLQRNGRDDPESVALV
                     DGALARMRDLAQDLRPPLLDEVGLAASLGWYVEREATRAGLAYRLHLAPLPERPPIAV
                     EMTCFRVAQEALTNIIRHAKAHTVDVELSPLEGAVLLVIRDDGQGFDVPAARRRATQG
                     MSQGLTSMQERVALAGGILEMESTPGAGTTIRARVPCGGIHG"
     gene            8162..8824
                     /locus_tag="VFU75_10740"
     CDS             8162..8824
                     /locus_tag="VFU75_10740"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5467358.1"
                     /translation="MAEAPLRVLLADDHALVRAGLRALLNDLPGVEVVAETGDGREAL
                     RLVRERKPDIAMIDISMPGLNGLDVAARITHDRPATRVIIVSMHGDDETVRRALTAGA
                     AGYVLKNSDRAELEMALRTVARGDTWLTPTLTKRVVAAFTQEAKSPEAGPFAALTPRQ
                     REVLQLVAEGFSNKEIAQRLDVAPKTIETHRTELMERLGIHGVAGLVRYAIRVGLVRP
                     GP"
     gene            8917..10830
                     /locus_tag="VFU75_10745"
     CDS             8917..10830
                     /locus_tag="VFU75_10745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5467359.1"
                     /translation="MAIRILLVEDIDEDAELAEVELRKAGIDFVLRRVMTPEEFRAAL
                     REHAPDLIISDHKLPQFSGEAALRLTQELGSPAPFILMTGSLDEETAVEYMKSGAADY
                     ILKDRRARLGNAVLSALDRRRVGEQLREREEYFRALIEQTHDIIAVLDGDAALKYASP
                     SVGPVLGYTPEELLGRSILDLVHPEDAEAMVRAFAEGVATGQGGRGLELRLRRKDGGY
                     RIIEAIGRYLLNDPVVGGVVINARDVTDRRLLERQLVQSQKMEAVGRLAGGVAHDFNN
                     VLTAILGYTGLLLDGLPALSPLRPDLDEIRNAANRATGLTRQLLAFSRKQALETRVLD
                     LNDLVKEMDRLLRRLLGEDVDVVTTLAPGVGQVRADAGQLEQVVVNLAVNARDAMPEG
                     GKLTIETQNVELDQSYAEGHLPVTPGRYVMLAVSDTGIGMSAETMSHVFEPFFTTKEA
                     GKGTGLGLATVYGIVKQSGGSVWCYSEPGQGTTFKVYLPRVDAPVDRVEPSAPAGPRH
                     GTETILLVEDETELRNMTRRLLEKHGYTVLEAASSEAALGIARLHPGPIHLLLTDVVL
                     PGESGRRLADELLVRRSELRALFMSGYTEEAIVHRGVLAANTAFIHKPFTGDGLAAKV
                     RQVLDARGPARSA"
     gene            complement(10843..>11029)
                     /locus_tag="VFU75_10750"
     CDS             complement(10843..>11029)
                     /locus_tag="VFU75_10750"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4440 domain-containing protein"
                     /protein_id="HEU5467360.1"
                     /translation="DRQQQFDQLQVRVYGSAAVVTGMVISTDLAGHEAKTVFTDVFAK
                     RGGRWQAVNAQENPVTK"
BASE COUNT         1877 a         3504 c         3749 g         1899 t
ORIGIN      
        1 tgccggtccc cggacacttg ctcagggctc atgtacgaag gcgtgcccat cgaaatgccg
       61 gtgcccgtga gccgctgcgg cccgccggcg ctcgcgacgg ccagcgcgat cccgaaatcg
      121 gcgagcatcg gctggccgtc atgcagcagg atgttctcgg gtttgatgtc gcggtgcagg
      181 acgccgtgcc ggtgcgcgta gtcgagtgca ctcgcgagcg cccggatcat gttgaccgtc
      241 tcttcgaccg gcaggggacc ctcgcgttcg aggcggccgc gcaacgatcc gccctgcacg
      301 aggggcatga cgtaatagag ggaactgccg atcgctcccg aatcgaagag cggcaggata
      361 tgcgggtgct ggagttgagc ggtgacccgg atttcgcgaa ggaaccggtc ggcgccgacg
      421 acggcgccga cttcggggcg gagcaccttg accgcgacct gccggtcgtg cttgaggtcg
      481 gtggcgaggt acaccatcgc catcccgccc tcgccgatct ggcggtcgag ctcgtaccgg
      541 ccggcgaggc cgttcctgag atcgtcgaag aggtcggtca cggtgtcaca aaactagacc
      601 agccccttcg taacctccag acagcggtcg atctcttctt ctgtattgaa gagggcaggg
      661 gcgatccgga tctgcccgtc ccgtacggtc acgtccaccg acgcgccctg aaaggctttc
      721 cggacatccg ccatcggctt cggagtgaac atggtcacga tgggggaccg gtttcccggg
      781 ggcgtgaaca gtttgtggcc ctgccgggtg agtccgtcgt gaaggcgctg gcccagggcc
      841 acggtgtgcg cctcgatgcg tccgacgcct acgccctgga ggtacgtgag ggctgccgag
      901 agctccttca ccggaccaaa agcccggctg cagtagtcaa acttccgggc ggtctgatcg
      961 agctcgaagt ggtagtcggg aagctccttc tcgacctgga attcgccgta ccggtcgatt
     1021 ttcaggcgct ccagtacctc cttccttata tagaagggag cgacgccaaa gccggccagc
     1081 atccacttgt acgaccctgc acagacggca tccacacccg ccgcttgcac gtccagctcg
     1141 atagttccga gtccctggat gacgtccgcg tacaacaccg ccccgtgcgc atgggccagg
     1201 tcggccaggg ggcgcagatc gtggcgaaag ccgttcaggt gggacaccca ggccaccgac
     1261 acgatgcggg ttttgtcatc gacaaggggc tcgaagtccg tcacactgac ggcgccgtcg
     1321 cggttccggg cgatccggag ctcgatgccc ttcgatgctt cgagggctcg atacagcacg
     1381 aattccgtcg ggtagtggag gtcgtcgatc acgacgttgt cccccgcgcg cagctccatt
     1441 ccctgcgcga tgatgttctc cccttcagcc gttgagaaca gcaagccgac ctcatcgggg
     1501 ctcgcattca ctagctgggc gaattggctc cgcaacttcc cgtcgccacc gagcagctct
     1561 ccgacctcca gtgggcgctt cgacttgttc tcgaggaact cggcacccgc ctgcaccacg
     1621 gcccgcggca cgggcgcgat gtacgccgag ttgaggtagg tccgttgggc ggtgatgggg
     1681 aagtcgtccc gaacgcccag gggatcgccg ggcgtgtgca catgaacctt cgcggccgca
     1741 aacgtagcgg cggtggtcgc gacgaaagac cggcggtcca tcactggcct ttcaccgtga
     1801 cgtagtagtt ctcatcgact gtcggagcgg cgcccgccgg ggcgatggtc agggtctgcc
     1861 actcctcagt cggcttcaac agatgtgttc ccgacccggg gacttccacc cggatcggca
     1921 tgtcgaaccc ggtcacgacg ttcgaccacc ggtacgacag ggtggtgccg tccaacttgt
     1981 agtcgaactc ggggaccttg gtggtgcgca ggtactggtc gaagaacttc gacagatcca
     2041 cgccggcctg ctgggagatg tacgtctcca cctggtgact cgagacggtc tgatgccaga
     2101 aggtcttctg gagcccccgg aggatcttgc gccagcgcgc gtcgtcatcc acgacttgcc
     2161 tgagggtgtt gaggacgttg gcgcccttca tgtagaggtc gttgtggtac caacggccga
     2221 ttccgtaggg cccgatgatc gggtagcggt tcgagatgct gcgccgaagt ccgatgaggt
     2281 acgcttcacc ggccgttttc cccttctggc actccgtgta gatcccttcg gagtaggtcg
     2341 cgaagctctc gtggatccac tgatcggcga catcgctcgc cgagaggttg ttgccccacc
     2401 attcgtgggc gctttcgtgg atgacgatgt agtcccagga aagtccgagt ccggtgcccg
     2461 agagatcgcg acccaggtag ccccgcagga acttgttgcc gtaggcgatg ccgctctggt
     2521 gttccatgcc caggtacgac tgctcgatga gcttgtaccc gtccttgtac cacgggtacg
     2581 ggccgtacca gtgctcgaag cactggatca tcggggtcgc ctgtttccac tgacctttcg
     2641 cggtgtcggt gtggtaggcc atggaccaga agtcgagcga gagcgggcca ctttcgccct
     2701 gcagggtatc cgagaagtgc gcgtactgtc ccgcattgac cgctacgtcg tacatgttga
     2761 tcggatcgtc cacgaaccac tcgtaggtcg tggtgtggtc ggcgttgtgc gtggtgctcc
     2821 ggagccggcc attcgagacg tcgatcatcg gatcgggcac ggtgatcgcg atccgctggc
     2881 tgtccggctc gtccgccgga aagtccttca ccggccacca gatgctcgct ccgatcccct
     2941 cgtcggaggt ggcgagccag gggtttccga gcgagtcctt cgcccatatg aacccggcgc
     3001 cgccgcgggg acgatcccgc ccgggctggc catggtagta gaccgtgatg gtcttcgcgt
     3061 caccggtgcg ttgtgcggcc ttgagcgtca cgaagaacgc gtcgccatcc cgccgtgagg
     3121 tgagtttctt ccggtcttgc acgatgctgt cgatggcgag cgggatctgc agatcgatct
     3181 gcatctcctg cgccggcttg aggaccctgt aggtgatgcc attccagccg cgcacactcg
     3241 aatccgccgg attgacgcgg acgtggaggt cgtaaaagga cgcgtcccac cacgcgcgcg
     3301 ccgggccatt ggagccgcgg agcgtgtccg catgggtgta gatggtatcg agcggggggc
     3361 gctgtgcagc cgccggaacg ctcaccgcca gaagaacaaa cagagcagct atggaacgca
     3421 tcgcgtggtc ccgattgtag gttcgggaag caacgctacg ccccgggtca ggggcgggca
     3481 agcaacgcgt gaccaagggg gaactcgccg agtcaatctg taacaccaga tgaccgcgtc
     3541 cttgcccgtc ctttccctca tcatcccggt acgtaacggg gcggagttcc tgccccacac
     3601 gttgggtgag ctccgggcct tcatgatgcg cgaaccgtgc gccgaggtcg tgctggtcga
     3661 cgacgggtcg gagccgcgga cggcggcgct gcttgccgcc ttcgcctgcg accagccgcg
     3721 cgtcacgctc ctggtgcacg aggtcaacca ggggaagggg gcggcggtgg cgcgcggaat
     3781 gctggccgcc cggggtcggg tgcgcgtgtt caccgacgcc gacctggcgt atcccctcga
     3841 tcaattgccc gccgtgtgtg ccgcgatcga ccagggctcg gaggtcgcca tcgcctgccg
     3901 ggtactcggc gagtcccggt atctcatgag tccgaccttc tttcattacc tctacacgcg
     3961 acacctgctg agccgggcgt acaacgccgt ggtccggtgg acgctccttc cggggattct
     4021 cgattcgcag gccggattga aagcattcag cgcgcgggct gcggaggtca ttttcccgcg
     4081 gatgacgatt cgggggttcg gattcgacgt cgaggcgtta ctcatcgcca gacgtcacgg
     4141 actggagacg gtgcaggtcc cggtccattt ccggtatgac tccgagccga cgacggtgcg
     4201 gttggcgcag gatggcggca ccctgctctc cgacctcgcg cgcatccgct ggaacgattt
     4261 gcgaggccgg tatcgctgag cgacgggtgg tcatcaacgc cgacgatttc ggaatctccg
     4321 acggcgtcac ccgggggatc gtcgaggcgc atcggggcgg ggtcgtcacc gcggcttcga
     4381 tgttcgcgaa cgctcccggt tttgccgcgg ccgtccaggc ggcacgcgag ctccctcgct
     4441 tggatctcgg tttgcatttc aacctcaccg ccggtgtccc ggtcgcgccc cctgcccagg
     4501 tctcgagtct gtgcggtgcc ggcggtgggt ttctccctct gggtgccctc attcggcgcg
     4561 cgctcgcggg gcgcgtcgat cccgacgacg tggcacggga atgccgggcg cagctggatc
     4621 gcttgcggac gaacgggatc gtcgtcagtc atctcgacgg acaccggcac gcgcacgtgt
     4681 tgccgagcgt ctggccgggc gtcgcagcgg tggcgcgttg tgaagggcta cccgtgcgga
     4741 agccggtcga accccttcgg cggggtcggc cagccacgaa gctcgcgttg ggcatagcgt
     4801 atgggttcgt gactcgacac gcgggggagg gaccgccgag cccccacttc cgggggatcg
     4861 gcctgctcgg cgcctggcac tttgcggagg cgctcatcga gctgatcgac catctccctc
     4921 cgggtgtgac ggaagtgatg gtgcaccctg gttacgtcga cgccgatctg ctgaggtggg
     4981 attcctacgg tcaggagcgt gagcgcgagc tcgtcgcgtt gcgctcgccc caggtattga
     5041 cccggcttac ccggggagac ccgcagctgg tgcggttcgt ggacctgtag ccctcattcg
     5101 ccacagatcc gcctgaatcc cgatccagca gagcaagcag ggaaccgcct gcacggagta
     5161 cgcgcgaagg acgtcgtgcc ggtagtgcca ggggaccagc gacgtcgcga ccagcgagac
     5221 gagcaccagg gtcagtatgg ccaggaccac ccgccaccgc tctccgaacg tgaccgccca
     5281 gatcccgatc cccgcggtcg caatcacata cgacggcgac tcggcctgat gattgaagat
     5341 cacgacgaag acgagcagtg agcagaggaa gaggcggcgg aactcaggat ggcgcgccgc
     5401 gcgtcgcagc aggatcacgg gcgccaggag ggtgacgagt ccggccagct gtagcggcca
     5461 gttggggccg gcatatccga accaggtgcc gagtatcccc agaacggact ccccacgcag
     5521 cacgctggtg gtcgtggtgg tcgtcagcca cgcgtggtac tgcgccacga gcatggacgg
     5581 cgcggtgacg agcagcggtg tgagggccag cagagcgcct gccaccacga atatcgcgat
     5641 ccgcttgagc ctgacacccg cgggttgggg cgaaaccagg gcgaagacac cgccggccgc
     5701 cgggaagatc ttgatgagcg cccccgcccc cgtcatgagg gcggcgccga cgatgctctc
     5761 ccgttcgcag gcgaggaacg tgaggatcag gagccccgcc acgagcgcat tgctctggga
     5821 gttctgcatc gatcggacga catccaagta aatgaacacg agcacaagct gggctcgccg
     5881 cgggtcgagc aggcgggcaa tggcccaggc gggggccgcg gcattgatcg cgttccagca
     5941 aaagaggccg aggaggggcg gcaagagggc gaagggcgcg aacagcaggg cgaacgccgg
     6001 gctgtagagg tagccgaagc agtccggcgt gttgaggtag agaccctgtc cgtggatgag
     6061 gcggtcgaac gtgcagcgga agagcagaaa gttgccgggg tggatcaatc ctttttgcaa
     6121 cgtgaccagc agtactgata ccgcgtagag gccccagatc catttcaacg gccttggagc
     6181 gggtgtgggt cgaccggtca ctgcgcggga atctacaccg ggcgcagcca tcggatggag
     6241 cggaacacct gtagggcacg aatttgcagg ggtggaccct cgggcgttcg cgaactagcg
     6301 aaccgtgtgt tagctatttg gacattgtcg accgagcctg gcatgtctga aacacctctc
     6361 ccgaccccga ccgcgccgct cactcccgac gctcaggatc ggctacttca cctcttcact
     6421 cagcagatcc ccgcacgcgc ctgtgccact gaccgtgatc tgcgcgtcgt gtgggataac
     6481 gggagtgcct tccccgacag tccctccgcg gtcggaaaaa ccgtctccga gctgtttgcg
     6541 gcctccccgg atcgcgaacg ggtggtcgag gcctgtgtcc gggccctgaa aggggagggc
     6601 acgacgctcg agatcaacga cggaacctcg gcggcgcatc tgcggctcgt accattccat
     6661 gattgggatg gtgagatcgt tggcgtcgtc ggcgtcgcgt tcgacaccac gagcctggtt
     6721 cgcagcaagg aggacgtcga gagcggccag ctccttcttc gacaggtgct cgacacgctc
     6781 ccggtcggtg tgttcgtgat gaatcgctcc ggtgacatcc tgctcgacaa ccccgcttcc
     6841 cggaatatct gggggggatc gatcaggtcc ggcccggagc gatgggcccg gagcatcgcc
     6901 gtttggcaca acagtgggaa gccgatcgct gcggaggaat gggcgtcgag acgcgcgatc
     6961 gcgagggggg atgtcagccg tgacgagctg atcgacattc agacccatga cggcgaaggg
     7021 cacaagacga tcgagaatta cgcggcgccc atacgcggtc ccaagggcga catcaccggt
     7081 gcggtcgtgg tcaaccagga tgtgacggag cgggtgcgtg ccgtggaggc gctccgcaag
     7141 accgaacggc tcctcgtcga cgccgagcgg cttggccaga ccggtggatg ggaacaggac
     7201 ctcgtcacgg gcgagatcgt caacaccgat gcgaacgccc gccttttttt cggagacgac
     7261 cggagcaagg gagcgcggct cgaggattac gccgacgccg tccaccccga cgatcgcgca
     7321 tgggtgatgg caagacgcga gcggatgctg gcgggaacgg gatcgacgga aatcgaatat
     7381 cgagttatct ggcccgacgg aaccgtgcat tgggtcttcg gacgggccac ggtggtgcgg
     7441 gatgagtctg ggaagccggt tcgtgtatac gggaccaacg cggatgtcac cggtcgaaag
     7501 ctggccgagg aggagctggg acggcgggcc cggcaactgg aagcgctgtc ccgccggctg
     7561 atcgaggctc aagaagccga gcggcgcgcg gtggcccgcg agctgcacga cgacttcgga
     7621 caggtgctca cggcgctcaa gctcaacctt cagcggaatg gacgtgacga tccggagagc
     7681 gtcgccctgg tggatggcgc cctcgcgcgc atgcgcgacc tcgctcagga tcttcgtccg
     7741 cccttgctcg atgaggtggg cctcgccgcg tcgctcggat ggtacgtgga acgtgaagcc
     7801 acccgggccg gtctcgcgta ccggctgcac ctcgccccgc tcccggagcg gccccccatc
     7861 gccgtggaga tgacgtgctt ccgcgtggcg caggaggcgt tgacgaacat catccggcac
     7921 gccaaggcac acacggtgga cgttgagctg agtccgctgg agggcgccgt actcctggtg
     7981 atccgtgacg acggccaggg cttcgacgtg ccggcggcgc gccgccgcgc cacgcagggg
     8041 atgagccaag ggctcaccag catgcaggag cgggtcgcac tggcgggtgg aattctcgag
     8101 atggagtcga cgccgggtgc cggaaccacc attcgcgcgc gcgtcccctg tggtggcatc
     8161 catggctgag gcgcctctgc gggtgctcct cgcggacgat cacgcactcg tgcgtgccgg
     8221 tctgcgggcg ctcctcaacg accttcccgg ggtcgaagtc gtggcggaaa ccggggatgg
     8281 acgagaagcg ctgcgtctcg tccgcgagcg gaaacccgac atcgccatga tcgatatttc
     8341 gatgccgggc ctgaatggcc tcgacgtcgc ggcccgcatt acccacgacc gccccgctac
     8401 gcgcgtcatc atcgtctcga tgcatggcga cgatgagacg gtccggcgcg ccctgacggc
     8461 cggagccgcc gggtacgtcc tcaagaactc cgaccgagcc gagctcgaga tggccttgcg
     8521 gaccgtcgct cggggggaca cgtggctcac gcccacgctg accaagcgcg tggtggcggc
     8581 cttcacccag gaggccaagt caccggaggc ggggcctttc gcggcgctca caccccgcca
     8641 gcgggaggtc ctccaactcg tggccgaggg cttctccaac aaggagatcg cgcagcggct
     8701 cgacgtagcc cccaagacca tcgaaaccca tcgcaccgag ctcatggagc gcctcggcat
     8761 tcatggcgtc gcgggcctgg tgcgctatgc cattcgggtc gggttggttc gccccgggcc
     8821 ctaggcggtc ccccgacagg taggtagttc ccctagccga accccggtct ttacccgact
     8881 tcgcccgggg cccgtttcac ctagccttgc tggcgtatgg ccatccgcat cctcctggtc
     8941 gaagatatcg acgaagacgc cgagctggct gaagtcgagc ttcgcaaagc agggatcgac
     9001 ttcgtgctcc gacgagtgat gacgccggag gagttccgcg ccgccctccg ggaacatgct
     9061 cccgatctca tcatctccga tcacaagctc ccccaattca gcggcgaagc cgcgctcagg
     9121 ctcacccagg agctgggatc gccggcgccc ttcatcctga tgactggatc gctcgacgag
     9181 gagaccgcgg tcgaatacat gaagtcgggc gccgcggact acatcctcaa ggaccggcgc
     9241 gcgcgtctcg gcaacgcggt cctgagcgca ttggaccggc gccgcgtggg cgagcagctc
     9301 cgcgagcgcg aggagtattt ccgggcgctg atcgagcaga cgcacgacat catcgcggta
     9361 ctcgatggtg acgcggcgct caagtatgcg agcccgtcgg tcggaccggt ccttgggtac
     9421 acgcccgaag agctgctggg ccggtcgatc cttgatctgg tgcacccgga ggacgccgag
     9481 gcaatggtcc gcgcctttgc ggaaggcgtg gctaccggcc agggtggccg tggactcgag
     9541 cttcggctcc ggcggaagga cggcgggtac cgtatcatcg aggcgatcgg ccgatacctc
     9601 ttgaacgacc ccgtcgtcgg cggcgtggtg atcaatgccc gtgacgtgac cgaccgtcgt
     9661 ttgctcgagc ggcagctggt ccaatcacag aaaatggagg cggtgggccg gctggcgggc
     9721 ggcgtcgcgc acgacttcaa caatgtcctc accgcgattc tggggtacac cggcctcctc
     9781 ctggacggcc taccggccct ttcccccttg cggccggatc tcgatgaaat ccgaaacgca
     9841 gccaaccggg ctaccggact cacgcggcag ctgctggcct tcagccggaa gcaagccctc
     9901 gagaccaggg ttctggacct caacgacctc gtcaaggaga tggaccggtt gctgcggcgc
     9961 ttgctgggcg aggacgtcga cgtggtgacg acgctcgccc cgggcgttgg tcaggtgcgg
    10021 gcggacgcgg ggcagttgga gcaggtcgtg gtgaacctcg ccgtgaacgc tcgcgatgcg
    10081 atgccggagg gtgggaagct gacgatcgag acccagaacg tcgagctgga ccagtcgtat
    10141 gccgagggac acctcccggt gacgccagga cggtacgtta tgctcgcggt ctccgacacg
    10201 gggattggca tgtccgccga gacgatgtcg cacgtgttcg agccgttctt cacgaccaag
    10261 gaggccggca agggcaccgg cctgggcctg gcgaccgtgt acggaattgt gaagcagagc
    10321 ggcggatcgg tatggtgcta cagcgagccg gggcagggca cgaccttcaa ggtgtacttg
    10381 ccgcgcgtgg acgctccggt ggatcgagtc gagccatccg caccggccgg tccaaggcat
    10441 ggcaccgaaa ccattcttct ggtggaggac gaaaccgagc tgcggaacat gacccgccgg
    10501 ctcctggaga agcacgggta taccgtcctg gaagcggcgt cgtcagaggc ggcgctcggc
    10561 attgcgcgcc tccatcccgg ccccattcac ctgctcttga cggatgtcgt gttgccagga
    10621 gagagcgggc gtcggctggc cgacgaactg ctcgtgcgac gtagcgagct gcgagccctg
    10681 ttcatgtcgg gctacaccga agaggcaatc gtccatcgtg gcgtgctcgc ggcgaacacg
    10741 gccttcattc acaagccttt cacgggcgat ggcctggccg cgaaagtccg tcaggtgctg
    10801 gatgcccggg gccccgccag gagcgcctga gaccggtgtc catcacttgg tcacggggtt
    10861 ctcctgcgca ttcaccgcct gccaccgccc gccccgcttt gcaaagacat ccgtgaatac
    10921 cgtcttcgcc tcatgacccg ccagatcggt cgaaatcacc atgccggtca ccacggcagc
    10981 ggagccgtag acacgcacct gcagttggtc gaactgctgc tgccggtct
//
LOCUS       DASUBP010000282         2705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_487635, whole genome shotgun sequence.
ACCESSION   DASUBP010000282 DASUBP010000000
VERSION     DASUBP010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2705
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_487635"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="VFU75_10755"
     CDS             complement(<1..1079)
                     /locus_tag="VFU75_10755"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HEU5467361.1"
                     /translation="MRTENALPAWIFAGALLVGVTADQLLRSAPWGVNITICTLLLVA
                     VAAALVKYHRLSVSRETWWLALTVVLLALAFARRDSRALAAFDFMALVATLGLTAATL
                     RGIRLSRLNVLDQALQVGATAAYTLVGALPLLGRLAQWMQEAKSAGFSRARSVALGAA
                     IATPVLLGFGILFASADPVFADAMQGIFTIDPGVAASHVFLITFFSGLSAGYLAWSIL
                     RPLQLPSRVPTVFRTGVGAVPVLTALGLTIGLFALFVAFQTGELFGGTAWVQRAAGPS
                     YAEYARSGFFELLTVAALVLPLLIVADRALGSHLGANGRWFRILARTLLALLLVIILS
                     ALNRMRLYVAAYGLSEIRLYATAAMA"
     gene            complement(1076..1723)
                     /gene="uppS"
                     /locus_tag="VFU75_10760"
     CDS             complement(1076..1723)
                     /gene="uppS"
                     /locus_tag="VFU75_10760"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HEU5467362.1"
                     /translation="MISNFVSQSALHVAIIMDGNGRWATRQGLPRAAGHRAGADAVHR
                     AVEAASQRDIRQLTLYAFSSDNWRRPLPEVLGLFNLFEEVIRDETANCVSRGVRLSVI
                     GRRDRLPPRLRAAIKSAERTTERGRRLHLRIAIDYSARAAIGDGGILPDVDLLIRTGG
                     ERRLSDFLLWECAYAELLFLDTMWPEFTAEELDRALGTFHARERRFGGLPGEMAG"
     gene            complement(1735..2112)
                     /locus_tag="VFU75_10765"
     CDS             complement(1735..2112)
                     /locus_tag="VFU75_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEU5467363.1"
                     /translation="MAKSGTAPASKPAKGDAPAPPAMSAAAQAAELDRLIHEKMRLGI
                     VSALASHESLSFNELKRALQTSDGNLSVHARKLEDAGYVSCTKTFEDRVPKTVYRLTA
                     TGRKALERYLDHMEALIRATRAP"
     gene            complement(2105..>2705)
                     /locus_tag="VFU75_10770"
     CDS             complement(2105..>2705)
                     /locus_tag="VFU75_10770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467364.1"
                     /translation="GLFSGVAAPQPLSDRAIENLRYIRETMTRSSSFTAVPGLGQVTI
                     GVTALGAAWLAHRQQTTLAWLSVWLAEALVALTLGGVTMVRKSRAVKDPMVTGPGRRV
                     LLGFLPAFGSAAVLTAPLVLAGVPALLPGVWMLLYGTGVVAGGAFSVRPVPLMGICFL
                     VVGTGALFTPAAWGDIWMATGFGGLHIVFGSLIARRYGG"
BASE COUNT          480 a          894 c          967 g          364 t
ORIGIN      
        1 gccatggccg cggtggcgta gagcctgatc tcggagaggc cgtacgcggc gacgtagagg
       61 cgcatccggt tcagggccga gaggatgatg acgaggagca gcgcgagcag cgtgcgggcg
      121 agtatccgga accaccgccc attcgcgccc aggtgactgc ccagggcgcg gtccgccacg
      181 atgagcaggg gcagcaccag cgccgccacc gtcaacaact cgaagaaccc cgaccgcgcg
      241 tactcggcgt agctcggtcc ggcggcacgc tggacccaag cggttcctcc gaacagctcc
      301 cccgtctgaa acgccacgaa gagcgcgaag aggccgatcg tcagccccag ggcggtcagc
      361 accggtacgg cgccgacacc cgtgcggaag acggtcggga cccgggaggg gagctggagg
      421 ggacgcagga tggaccaggc gaggtagccg gcgctcagcc ccgagaagaa ggtgatcagg
      481 aagacgtgag atgcggcgac gccgggatcg atggtgaaga tgccctgcat cgcgtccgcg
      541 aaaaccgggt cggcgctggc gaacaggatt ccgaaaccga gcagaacggg tgtggcgatg
      601 gcggctccca gcgccaccga acgggcccgc gaaaatccag ccgacttcgc ttcctgcatc
      661 cactgggcga ggcgtcccag cagcgggagg gccccgacga gcgtgtaggc cgcggtggcc
      721 ccgacctgga gggcctgatc gagcacattg agacgcgata gccggatgcc ccggagcgtc
      781 gccgccgtga gcccgagcgt ggccaccaga gccatgaagt cgaacgccgc gagggctcgc
      841 gaatcccggc gcgcgaaggc caaagccagc agcacgacgg tgagcgcgag ccaccaggtt
      901 tcccggctga ccgagaggcg atggtacttc accagcgcgg cggcgacggc gaccagcagg
      961 agcgtgcaga tggtgatgtt gacgccccaa ggggcactcc tgaggagctg atcggcggtg
     1021 acccccacca ggagcgcacc cgcgaaaatc caggccggca atgcattttc ggttctcatc
     1081 ctgccatctc ccccggcagg ccgccgaaac gacgttcgcg ggcgtgaaag gtgccgaggg
     1141 cgcggtcgag ctcttcggcg gtgaactcgg gccacatcgt gtcgaggaag agcagctcgg
     1201 cgtaggcgca ctcccagagc aggaagtccg agagccggcg ctcgccgcca gtccggatga
     1261 ggaggtcgac gtccgggagg atcccaccgt cgccgatggc ggcgcgtgcc gagtagtcga
     1321 tcgcgatgcg gagatgcaac cggcgcccgc gctccgtcgt ccgctcggcg ctcttgattg
     1381 cggcgcggag acggggcggc aggcggtcgc gccgtccgat gaccgacagg cggacgccgc
     1441 ggctgacgca gttggcggtc tcatcccgga tgacttcctc gaaaaggttg aacaggccga
     1501 ggacctcggg gaggggacgg cgccagttgt cggacgagaa ggcgtagagc gtcagctggc
     1561 ggatgtcgcg ttgggacgcg gcctcgacgg ctcgatgtac ggcgtcggcg cccgcgcggt
     1621 gaccggcggc gcggggaagt ccctgtcggg tggcccagcg tccgttgccg tccatgatga
     1681 tggctacgtg cagagcactt tgagatacaa agttagagat caaaaaaaaa tcgctcaggg
     1741 ggcgcgcgtg gcccggatga gcgcctccat gtggtcgagg taccgctcga gtgctttccg
     1801 cccggtggcg gtgaggcggt acacggtctt cgggacccgg tcttcgaatg tcttggtgca
     1861 gctgacgtag ccggcgtctt ctagcttgcg cgcgtggacg ctgaggttgc cgtccgaggt
     1921 ctgcagggcg cgcttcagtt cgttgaagct cagcgattcg tgactcgcga gcgcgctcac
     1981 gatgcccagc cgcatctttt cgtgaatgag ccggtcgagc tccgccgcct gcgccgcggc
     2041 ggacatcgcc gggggcgccg gtgcatcgcc cttcgccggc ttgcttgccg gtgccgtgcc
     2101 actcttagcc accgtacctc cgtgcgatca ggctgccgaa cacgatatgc aacccgccga
     2161 agccggtcgc catccagatg tctccccacg cggccggcgt gaacagggcg ccggtgccga
     2221 ccaccaggaa gcagatcccc atcaacggca ccggccggac cgagaacgcg cctcccgcga
     2281 ccacgcctgt gccgtacaga agcatccaga cgccggggag cagggccggg actcccgcca
     2341 gcacgagcgg agccgtcagc accgccgccg atccgaacgc cggcagaaac ccgaggagca
     2401 cccgccggcc cggtcccgtc accatcgggt ctttcaccgc gcgtgacttc cggaccatgg
     2461 tcacgccgcc gagggtcagt gccactagtg cttccgccag ccacaccgac agccaggcga
     2521 gcgtcgtttg ctgccggtgc gcgagccatg cggcgcccag cgcggtcacc ccgatcgtca
     2581 cctggccgag ccccgggacc gcggtgaacg atgacgagcg agtcatcgtc tcccggatgt
     2641 agcgcaggtt ctcgatcgcg cggtcggaga ggggctgggg agcggccacg ccggagaata
     2701 ggccc
//
LOCUS       DASUBP010000283        10224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1087279, whole genome shotgun sequence.
ACCESSION   DASUBP010000283 DASUBP010000000
VERSION     DASUBP010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 10224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10224
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087279"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..346
                     /locus_tag="VFU75_10775"
     CDS             62..346
                     /locus_tag="VFU75_10775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467365.1"
                     /translation="MPALPARYSSPDERPAVVHDLPPIKFEGQGVTVRLAVRRTEDGT
                     WRGRIHFGAPDTEHERTTAEIFCAANEPDLWQSVRDLRDHHLRDLYRSLL"
     gene            355..540
                     /locus_tag="VFU75_10780"
     CDS             355..540
                     /locus_tag="VFU75_10780"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEU5467366.1"
                     /translation="MADDPIAKLRHDLANPLSAILAETQLMLLNEAQLDGELVESLKH
                     IEGLARRMRDILQDRRG"
     gene            complement(596..2455)
                     /locus_tag="VFU75_10785"
     CDS             complement(596..2455)
                     /locus_tag="VFU75_10785"
                     /inference="COORDINATES: protein motif:HMM:NF014052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage II sporulation protein M"
                     /protein_id="HEU5467367.1"
                     /translation="MTAATRDPGRPVVGDLRTQLEVETPEQVEVRFELAGLGSRVGAF
                     VIDLIVITLGFAVLTIVLAALMPSRGAGNTGGTAGTWVAALLYLIGSFAALLYFTLFE
                     GLGGGRTPGKRSLGIRVLMDTGRRITFGAAALRNILRFIDFFFPFVPLLPGMLFVFLS
                     KSRKRLGDMAAGTIVVRDRPTDWTPLAISRKPEQPAELEIGPPELSEEEFRLLDRFVA
                     RAQDLAPAALSKMTGDLVERFHEWIPRSPDGMTGLTMLLELERSRRRGKFSTRARAGM
                     AGRVAVTGERFVERRRDRWEAFRSVALRIEQQGVRGLPAEEIPGFAARYREVAADLAR
                     ARTYGVDPQVIEYLERLVSAGHNALYRWRGKDRTPWWRYVSRDFPAAVLHSWRYVLVS
                     FLLFMVPAVSGYALLRARPDLADDLANPVLVSRAQEAADREARGVGYAQSRDQELPVI
                     AAAIITNNINVCFMAFAGGLALGIWTIISLTFNGFELGMGFGVFANYHAAGYLGTFIA
                     GHGVLELTAIFISGGAGLRLGHALIAPGDRTRRDALVVDGLIAAKMIGMVIVLLCIAG
                     TIEGLLSASDAPAVFKFLVSGTSAVFLGLYLANGWRHRVEAGQRSQPGRAALQ"
     gene            2497..3330
                     /locus_tag="VFU75_10790"
     CDS             2497..3330
                     /locus_tag="VFU75_10790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467368.1"
                     /translation="MVELKPMEMGEILDGGLTLYRRHFGLLIRLGVIALWLPASINIY
                     LQVTGGALQHSVLYLIVALLQYFCGLFLTAGAIRIISDSYLGKETALADAVGLGASKI
                     MPLFGVGLGKGLLLGLIVVVIGMLAGVGVPLMSGSGVTGLLFFAIVFIGAIWLLAFVA
                     CGYAVTTQVVVLEPLDGAFDSFGRSWELTKGFKRKVANIAILAGIIFNIPVAVVSGLA
                     QGLAASSPGTSAVLAVVAALLPIVLTPLLACVFTLMYYDLRIRREAFDLQVLSQQLVS
                     S"
     gene            3384..3956
                     /locus_tag="VFU75_10795"
     CDS             3384..3956
                     /locus_tag="VFU75_10795"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HEU5467369.1"
                     /translation="MFSAGRYHWVERRHPLQWLGDAWDRFQTWVNRLSAAHPGLSWVM
                     ITAACVLLVVLLTHIGYTLWKVYSVTVRPADLPARGATGVPLLDARSHRTQAEALARE
                     GRYTEALAHRFAALICELDEAKAVTIHHSKTPAEYAREARLDTTGRGTMADLVSRLYS
                     HLFGAAPIDEAGYRDFVTHTELVMPHVGPR"
     gene            3940..5169
                     /locus_tag="VFU75_10800"
     CDS             3940..5169
                     /locus_tag="VFU75_10800"
                     /inference="COORDINATES: protein motif:HMM:NF025617.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4350 domain-containing protein"
                     /protein_id="HEU5467370.1"
                     /translation="MSVRAEIGLALLLALGITVAMVVGKKSGAPAAAYEPASTFASGP
                     SGSRGPYEVLQELGVKVERRRIPFFDLNSEKDHHPQILVILDLEPGERPLESSEIKQV
                     AQFVQSGGMLVLAGYTGGIARCLPWAIRTQTSYRPDSFPVAPVAGRDLPDVTNFFQPR
                     RDSVAPGEGTHCPVFGVATTDTVARTQSGRPVVLDLRYRDGGRIVFLADDGWFRNAAW
                     KTTNVPYVLAPMIQSAARDRLVAFDEYHHGFASSSAPVLTITWLMGTPVGWFLLQILV
                     VGLVWLVVSAFRFGPALPVIDRRRRSPLEHLDALAAGLESSEGSQTALGLIVGGLRRR
                     LSRTGEGPAAKANEQWIAALMLAMPTAQGRGAVRRLREAFTKPGGGERVLAAANSVED
                     VWEELRPRTTRDAFSKP"
     gene            5118..6074
                     /locus_tag="VFU75_10805"
     CDS             5118..6074
                     /locus_tag="VFU75_10805"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEU5467371.1"
                     /translation="MGGTTSAHDARRILEALRGVVLGQDQPLRDVMVCLLARGHVLLE
                     GVPGTAKTLLVRALALALDVRYARIQFTPDLMPADITGISMLTGTHEFTFRPGPLFAD
                     LVLADEINRAPAKTQAALLEAMQERTVTVDGTTHPLPGVFTVFATQNPVEVEGTYPLP
                     EAELDRFMAKVLVGYPDAAVEQGILTRYVEGFEADRPATYGIKPITDGAGLEALRTAT
                     ELIRVEPRITAYITAIIRATRGAASLTLGASPRAGVSLLKASRALALLEERDYVIPDD
                     VKGLAPAVLRHRITVAPELELEGVTPDAALKAILEKVEVPTA"
     gene            6071..7402
                     /locus_tag="VFU75_10810"
     CDS             6071..7402
                     /locus_tag="VFU75_10810"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEU5467372.1"
                     /translation="MILYTRRALIALAAVAGVALLGFWVPGAISAMVLIDAVLVVLSL
                     LDAVYSVPLAGTLEKRGVSVRRDPPPAITVGHPTEVSYIWTNTSRRKARLRIRETRPD
                     LLGGTLPPRSLVIPARGEVVERLSVIGLHRGHEEAGQMYIDSMGPLGLGRARRRIPLP
                     WAVTVFPPLVSLRLRASVAQALRKRDAGMKPIRRLGEGRFFESLREWVPGDDLRHIDW
                     KATARRRKVITRQYEEERRQQLLLVMDTGRLMTADVAGVARLDYAVQAALELAYAAVQ
                     HDDNVGVMSFADGVKHFVAPGRGRAGLKRVLDVLTVIAPTLVEPDYPGAFRYLAARNR
                     KRALTVVFTDLIDRTASEALVANLATLRPRHLPLAVTLRNPELDLVATTRPGDVRDAY
                     RRAAAEELLRAREEALGHLRRAGVLVLDVPPARAAQAVVREYLELKRRGRL"
     gene            7479..9191
                     /locus_tag="VFU75_10815"
     CDS             7479..9191
                     /locus_tag="VFU75_10815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467373.1"
                     /translation="MRRVSRVSFAALAAAILAGVTCSFPTDQSDQVFVTVTLSAPLVV
                     QGATITAHGHAFRKTGSGNVPVNDVDFQWSVSDSNVARIVNDGRGTATITGVNAGLVH
                     VVARTAVYSKAADMDSMIRVARALEIDSIKPATIRYGGRITVYGVGINNIFLAELGPA
                     ILLADTFSFAGNRNGLGHMQFWVPFPAHSDQMIAFGPGVFATAAESTFVNPVDIFEPN
                     DTNPALIDLDSLNPIPQLPALRFFNPALFFEPFDQKSTSFGIDWYRFGQADTTQPATF
                     VFSAPGAGRDTTLFSIMADSIYNNGGFAYLDPNSWILAPNLGVFGCRSNSFFTPVASS
                     ESTVVEFGNLTSKKFMLYEEYTQSGGYEMRVIRGLVRADPTIGPDRFAPDELCNQADV
                     NFANPATHIAVHLGSAPFMDTTLTIDFPHAADWYRFRLDTVNAADVSENVTFATVSRS
                     PGSKDTSDIDVTVLDINGNYMGQVVDSGSTEVMTLGLTTNADYYVVISDYAGAPVRYG
                     LCIQVATSCPSLPGPVAGVAPGMTGSIPRRALRERMSPALRAAAQLRALRAAQAARIP
                     FRLP"
     gene            9191..>10224
                     /locus_tag="VFU75_10820"
     CDS             9191..>10224
                     /locus_tag="VFU75_10820"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5467374.1"
                     /translation="MECTKCHTPLPDNSRFCFACGADQTGGGDPAATSGQISSLMTRL
                     QRIVEGKYKLERLLGKGGMGAVFLAKDLTLDRDVAIKVLPPDVSQDDHVVKRFQQEAK
                     TAAKLDHTNIIPIFRVESEGGLNYFVMKYISGTSLEDLLEQKKPLPVEEIQRILWQAG
                     VALGHAHQRGIVHRDVKPANIMFDHDGKVMLTDFGISKALQAASGFTGTGMIIGTPHY
                     MAPEQAKGQPVDGRADQYSLGVVGYRMITGALPFAGDSIHTILYKHIFEEPPPVRSVR
                     PDIPEFLSTAIQRAMSKEPAQRYATMEDFATAVFPEQPVAAGRSGGPITPKPVARAPV
                     TAESPTEAFIS"
BASE COUNT         1619 a         3542 c         3364 g         1699 t
ORIGIN      
        1 ctaaggtatt gcgtgggtag aggttgttct tatcttagtc caaactcgcc ccagggtgct
       61 gatgcccgcc ttaccggcac gatacagttc tcccgacgag cgccccgccg tcgttcatga
      121 tctccccccc ataaaattcg aagggcaagg tgtcaccgtc cgtctcgcgg tccgtcggac
      181 cgaggatggc acgtggcggg ggcgcattca tttcggggcg ccggataccg aacacgagcg
      241 cacgacggcg gaaatcttct gcgccgccaa cgaacccgac ctttggcaat ccgtgcggga
      301 cctgcgggat catcacctgc gggacctcta ccggtcgctg ctctaggcca cgacttggcc
      361 gacgacccga tcgcgaagct ccgccacgac ctggccaacc cgctttcggc catccttgcc
      421 gagacgcagc tcatgctgtt gaacgaagct cagctggacg gcgagctcgt cgaatcgctc
      481 aagcatatcg agggactcgc gcgccggatg cgcgacatcc tccaggacag gcgcggctga
      541 gccgaagaca tggcccgcgg ttcggccgcc ggggtcggtg gccgagcccc cctcgttact
      601 ggagggcggc gcgccccggc tggctccgct ggcccgcctc gacccggtgc cgccatccgt
      661 tcgcgagata gagccccagg aagaccgctg aagtcccgct caccaggaac ttgaacaccg
      721 ccggcgcgtc actggccgag agcagcccct cgatggtccc cgcgatgcag agcagcacga
      781 tcaccatccc gatcatcttc gccgcgatga gcccgtccac gacgagcgcg tcccgcctcg
      841 tgcggtctcc gggtgcgatc agggcatgac ccaaccgcag ccccgcgcca cccgagatga
      901 agatggccgt cagctcgagt acgccgtgcc ccgcgatgaa cgtccccagg tatccagccg
      961 cgtggtagtt cgcgaatacg ccgaagccca tcccgagctc aaagccgttg aacgtcagcg
     1021 agatgatggt ccagatgccg agggccaggc cgccggcgaa ggccatgaag cagacgttga
     1081 tgttgttggt gatgatcgcg gccgcgatga ccgggagctc ctgatcccgt gactgcgcgt
     1141 acccgacgcc gcgggcttcc cggtccgccg cctcctgcgc ccggctgacg agcaccgggt
     1201 tcgccaggtc gtcggcgaga tcggggcggg cgcggagcag ggcatacccg gataccgcgg
     1261 gcaccatgaa caagaggaac gacaccagga cgtaccgcca cgaatgcagc acggcggcgg
     1321 ggaagtcgcg actgacgtaa cgccaccagg gcgtccggtc ctttccgcgc cagcgataca
     1381 atgcgttgtg cccggcggac accaggcgtt ccagatactc gatgacctga gggtcgacgc
     1441 cgtaggtccg cgcgcgggcc aggtccgcgg cgacctcgcg gtaccgggcc gcgaatcccg
     1501 gaatctcttc cgccggcaga ccgcgcaccc cttgctgctc gatgcgcagc gccacgctcc
     1561 ggaacgcttc ccaccggtcc cggcgccgtt ccacgaaccg ctccccggtc accgcgacgc
     1621 gccctgccat ccccgcccgg gcccgcgtgc tgaacttccc gcgtcgccgg gagcgctcca
     1681 attcgagcag catcgtcaga cccgtcatcc cgtccggcga tcgcgggatc cactcgtgga
     1741 accgttcgac caggtccccg gtcatcttcg agagtgccgc cggcgcgaga tcctgcgcgc
     1801 gcgcgacgaa ccggtcgagc aggcggaact cctcctccga caactccggg ggaccgatct
     1861 cgagctccgc cggctgctcc ggtttccggg agatggccag cggcgtccag tcggtagggc
     1921 ggtcccgcac gacgatggtc ccggccgcca tgtcgcccaa tcgcttccgg ctcttcgaga
     1981 gaaagacgaa gagcatgccg ggcagcaacg ggacgaacgg aaagaagaag tcgatgaagc
     2041 gaaggatgtt gcggagggcg gcggcaccga acgtgatccg gcgccccgta tccatgagta
     2101 cccggatgcc gagagatcgc ttgcccggtg ttcgcccgcc gccgagcccc tcgaacaatg
     2161 tgaaatacag gagggccgca aagctgccga tgaggtaaag aagcgccgcg acccacgtgc
     2221 cggcggttcc gcctgtgttg cccgcgccac gggacggcat cagggccgcg agcacgatgg
     2281 tgagcaccgc gaagccgagc gtgatcacga tcaggtcgat cacgaaagcg ccgacccgtg
     2341 agcccagccc cgccaactcg aaacggacct cgacctgctc cggagtctcg acttcgagtt
     2401 gagtccgcag gtctccaaca accggacggc cggggtctcg cgttgcagcg gtcacggtct
     2461 catcttatcg ccatcccgaa cgtggggcga gagcgcatgg tcgagctgaa gccgatggaa
     2521 atgggcgaga tcctggacgg tgggttgacc ctctatcgcc ggcactttgg actgctgatt
     2581 cgtctgggcg tcatcgccct ctggctcccc gcgtccatca acatctactt gcaggtgacc
     2641 ggcggagcgc tgcagcactc cgtcctgtat ctgatcgtgg cgctcctcca gtacttctgc
     2701 gggctcttcc tcacggccgg cgcgatccgg atcatctcgg actcgtacct cgggaaggag
     2761 accgccctcg ccgatgccgt cgggctcggt gcctcgaaga tcatgcccct gttcggtgtc
     2821 gggctcggga agggcctcct gctcggcctc attgtcgtcg tgatcggcat gcttgcgggc
     2881 gtgggggtcc cgttgatgtc cgggtcaggg gtcacgggcc tcctcttttt cgccatcgtg
     2941 ttcatcgggg ccatctggct gctggcattc gttgcctgcg gctacgccgt caccactcag
     3001 gtggtcgtgc tcgagccgct ggatggggca ttcgattcct tcggccgctc gtgggagctt
     3061 accaaaggct tcaagcgcaa agtggccaac atcgcgattc tggcgggcat catcttcaac
     3121 attccagtcg ccgtggtctc gggactggcg cagggtctcg cggcctcgag cccggggacg
     3181 tcggccgtgc tcgccgtggt ggcggcactc ctcccgatcg ttctgacgcc cctcctcgcc
     3241 tgcgtcttca ccctgatgta ctacgatctg cggatccggc gggaagcgtt cgacctgcag
     3301 gtgctgagcc agcagctggt gtcctcctga ccgtcgcgcc gctttcaccg cccgccgact
     3361 cgctgcggcg ggcgctggac aaggtgttct cggcggggcg gtaccactgg gtcgagcgcc
     3421 gccaccccct gcaatggctc ggggacgcgt gggaccgctt tcagacctgg gtcaaccgct
     3481 tgagcgccgc gcaccccggt ctctcgtggg tgatgatcac cgctgcctgc gtgctgctcg
     3541 tggtactgct cacccatatc gggtacaccc tgtggaaggt gtatagcgtc acggttcgcc
     3601 ccgccgatct tccggcgcgg ggcgccacgg gcgttcccct cctcgacgcg cgctcccatc
     3661 ggacccaggc cgaggcgctc gcgcgtgagg ggcggtacac ggaggcgctg gcccaccggt
     3721 tcgccgcact gatctgcgag ctcgacgagg ccaaggccgt caccatccac cactccaaga
     3781 caccggcgga gtacgcgcgc gaagcccgcc tcgacaccac gggacgggga accatggcgg
     3841 acctggtctc ccgcctctac tcccacctgt tcggcgccgc gccgatcgac gaggccggct
     3901 atcgcgactt cgtgacgcac accgagctgg tgatgccgca tgtcggtccg cgctgaaatc
     3961 gggctcgcgc tcctcctcgc cctcggcatc acggtggcga tggtggtggg gaagaagagc
     4021 ggcgcgcccg ccgcggcgta cgagccggca tccacattcg cgtcggggcc gtcgggttcg
     4081 cgggggccgt acgaggtgct ccaggaactc ggggtcaaag tcgagcggcg gcggattccg
     4141 ttttttgacc tcaacagtga aaaggaccac cacccccaga tcctcgtgat tctggacctt
     4201 gagccgggag agcggccgct ggaatcgtcg gagatcaagc aggtggctca attcgtccag
     4261 tcggggggca tgctggtcct ggctgggtac acgggcggca tcgcgcgatg cctcccgtgg
     4321 gcgatccgta cgcagacgtc gtaccgcccg gacagcttcc cggtggcgcc cgtcgccggc
     4381 cgtgatctgc ccgacgtcac gaatttcttc cagccgcgcc gggattccgt cgcaccgggc
     4441 gagggcaccc actgccctgt cttcggcgtg gcgaccacgg acaccgtcgc gcggacgcag
     4501 tccggccggc cggtcgtgct cgatctgcgc taccgcgacg gggggcgcat cgtctttctc
     4561 gccgacgacg gctggttccg gaacgccgcc tggaagacga cgaacgtgcc ctacgtcctg
     4621 gcgccgatga tccagtcggc ggcgcgcgac cgtctggtgg ccttcgacga gtatcatcac
     4681 ggcttcgcct cgtcgagcgc accggtgctg accatcacct ggttgatggg gactccggtg
     4741 gggtggttcc tgctgcagat tctcgtcgtc gggctcgtct ggctggtggt gtctgccttc
     4801 cggttcggcc cggccctgcc cgtgatcgat cgccgccgcc gttcgccgct cgaacacctg
     4861 gatgcgctcg cggccggcct cgagagttcc gaaggatcgc aaaccgcgct cggccttatc
     4921 gtggggggcc tccgccggcg cctgtcgcgg acgggggaag gacccgccgc gaaagcgaac
     4981 gagcagtgga tcgccgcctt gatgctggcc atgcctacgg cgcaggggcg cggggcggtc
     5041 cggcggttac gggaagcgtt caccaaaccc ggcgggggtg agcgcgtgct cgccgccgcc
     5101 aactccgtgg aggacgtgtg ggaggaacta cgtccgcgca cgacgcgcga cgcattctcg
     5161 aagccctgag gggcgtggtc ctgggacagg accagccgct gcgggacgtg atggtctgtc
     5221 tgctcgcgcg cggccacgtg ctgctcgagg gggtgccggg caccgcgaag acgctgctgg
     5281 tacgcgcgct tgccctcgcg ctggatgtgc gctacgcccg catccagttc actcccgacc
     5341 tgatgcccgc ggacatcacg ggcatctcga tgctcacggg cacccacgag ttcacatttc
     5401 gtcccggccc gctcttcgcc gacctcgtgc tcgccgacga gatcaaccgc gcgcccgcca
     5461 agacgcaggc cgcgctgctc gaagcgatgc aggagcgcac cgtgacggtg gacgggacga
     5521 cgcacccgct ccccggcgtc ttcacggtgt tcgcgaccca gaatccggtg gaggtcgagg
     5581 gcacctaccc gctgccggaa gccgaattgg accggttcat ggcgaaggtg ctggtgggat
     5641 atccggacgc cgcggtggag caggggattc tcacgcgcta tgtcgagggg ttcgaggcgg
     5701 accgccccgc gacctacggc atcaagccca tcaccgacgg cgccggtctc gaggcgctgc
     5761 gcacggcgac ggagctgatt cgcgtcgagc cgcggatcac ggcgtacatc acggccatca
     5821 ttcgcgcgac ccgcggcgcc gcgtccctga cgctgggcgc cagcccgcgc gcgggggtct
     5881 cgctcctcaa agcctcccgc gccctcgcgt tgctggaaga acgggactat gtgattcccg
     5941 acgatgtgaa gggcctggcc cccgcggtac tgcggcaccg gatcaccgtc gcgcccgagc
     6001 tcgagctcga aggcgtcacg cccgatgccg cgctcaaggc gattctcgag aaggtcgagg
     6061 tcccgaccgc gtgatcctgt acactcggcg ggcgctgatc gcgctcgccg cggtcgccgg
     6121 cgtcgcgctc ctggggttct gggtccccgg agcgatttcg gcgatggtgc tgatcgacgc
     6181 tgtgctggtg gtcctgtcgc tcctggatgc ggtgtacagt gtgccgctgg cgggcacact
     6241 cgagaagcgg ggcgtgtcgg tacgccgcga cccgccaccc gcgattacgg tgggccaccc
     6301 cacggaggtc agctacatct ggacgaacac cagccgccgg aaagcgcgcc tccgcatccg
     6361 tgagacccgc cccgacctcc tgggcggaac gctcccgccc cgttccctcg tcattccggc
     6421 gcggggtgag gtggtggaac ggctgagtgt gatcggcctg caccggggtc acgaagaagc
     6481 aggccagatg tacatcgact cgatgggccc cctcggcctg ggccgggcgc gtcgccggat
     6541 cccgctcccc tgggcggtca ccgtctttcc gcccctggtc tccctgcggc tccgggcgtc
     6601 ggtcgcgcag gcgctgagga aacgcgatgc cggcatgaaa ccgatccgcc ggttgggtga
     6661 agggcgcttc ttcgagagct tgcgcgagtg ggtgcccggg gacgatctgc ggcacatcga
     6721 ctggaaggcg accgcgcgcc gccgcaaggt catcacgcgg cagtatgagg aagagcgccg
     6781 gcaacagctc ttgctggtga tggacacggg ccgcctgatg accgccgacg tcgcgggcgt
     6841 ggcacggctc gactatgcgg tgcaggcggc gctcgagctg gcgtacgccg cggtccagca
     6901 tgacgacaac gtgggcgtca tgagcttcgc cgatggggtg aagcacttcg tcgcgccggg
     6961 gcgggggagg gcgggactga agcgcgtcct cgacgtgctg accgtgattg cgccgacgct
     7021 cgtcgaaccc gactacccgg gcgccttccg gtaccttgcc gcccgcaacc gcaaacgcgc
     7081 gctcaccgtg gtgttcaccg atctgatcga ccgcaccgcc tcggaagcgc tggtcgccaa
     7141 tcttgcgacg ctccgccccc gacatctccc cctcgccgtg accctcagga atcctgagct
     7201 cgacctcgtc gccaccacgc gtcccgggga cgtacgcgat gcgtaccgtc gcgccgcggc
     7261 cgaggagctt cttcgggccc gtgaggaggc actcggccat ctgcgccgcg ccggcgtgct
     7321 cgttctggac gtcccccccg ctcgagccgc ccaagcggta gtgcgagagt accttgaatt
     7381 gaagcggcga gggcgcctgt agcctgcccg cgtcgggtgt ctggtggggg ccactccaaa
     7441 gtcgtaagtt ggtacctcac tcgaagatgg gccctgccat gcgacgtgtc tcccgggtgt
     7501 cttttgccgc actcgccgcc gccatcctgg cgggggtgac ctgctctttc cccaccgacc
     7561 agagcgatca ggtcttcgtg accgtgaccc tgtccgcccc cctggtggta cagggagcga
     7621 cgatcacggc gcacggacac gcgttccgga agaccggttc ggggaacgtt ccggtcaacg
     7681 acgtggactt ccagtggagt gtgtccgact cgaatgtcgc ccgcatcgtc aatgacggcc
     7741 gcgggacggc gaccatcacc ggggtcaacg ccggactggt gcacgttgtg gcgcggaccg
     7801 ccgtctactc caaggccgcg gacatggaca gtatgatccg cgtggcgcgc gcgctcgaga
     7861 ttgacagcat caagccggcc acgatccggt acggtgggcg gatcacggta tacggtgtcg
     7921 gcatcaacaa catcttcctg gcggagctcg ggcccgcgat cctcctggcc gacacgttct
     7981 cgttcgccgg aaaccgcaat gggctcgggc acatgcagtt ctgggtgccc ttcccggcgc
     8041 actcggatca gatgatcgcc ttcggccccg gggtgttcgc gacggcggcc gagagcacgt
     8101 tcgtcaatcc ggtcgacatc ttcgagccga acgacaccaa tcccgcgctc atcgatctcg
     8161 attctctgaa cccgattcct cagctgccgg cccttcgttt cttcaatccg gcgctgttct
     8221 tcgagccgtt cgaccaaaag agtacgtcct ttggaatcga ctggtaccgg ttcgggcagg
     8281 ccgacaccac gcaaccagcg acattcgtct tttccgcccc cggcgccggc cgggatacga
     8341 cgctgttcag catcatggcc gactcgatct acaacaacgg cgggttcgct tacctcgatc
     8401 cgaactcgtg gatcctcgcg ccgaacctcg gggtcttcgg ctgccgttcg aacagcttct
     8461 tcactccagt tgccagctcc gaatcgaccg tggtggagtt cgggaacctg accagcaaga
     8521 agttcatgct gtacgaggag tacacccagt cgggcgggta cgagatgcgg gtgatccggg
     8581 ggttggtccg ggccgacccg accatcgggc ccgaccggtt cgcgcctgac gaactctgca
     8641 accaggcgga cgtgaatttc gccaaccccg cgacccacat cgcggtgcac ctcggttccg
     8701 ccccgttcat ggatacgacg cttacgatcg atttcccgca tgccgccgac tggtaccgct
     8761 tccggctgga cacggtgaac gccgcggacg tgtccgagaa tgtcacgttc gcgaccgtga
     8821 gccgctcccc gggttccaag gacacgtccg acatcgacgt cacggtgctg gacatcaacg
     8881 gcaactacat ggggcaggtg gtggacagcg ggtcgaccga ggtgatgacc ctcggcctca
     8941 cgaccaacgc cgactactac gtcgtcattt cggactacgc gggcgcgccc gtgcggtatg
     9001 gcctctgcat acaggtcgcc accagctgcc cgtcgctgcc gggccccgtg gccggcgtcg
     9061 ctccgggcat gaccggctcc attcccaggc gggcgctgcg tgagcgaatg agtcccgcgt
     9121 tacgcgccgc ggcacagctg cgtgcgctcc gcgccgctca ggcagcgagg atcccattcc
     9181 ggcttccgta atggaatgca ccaagtgcca caccccgctg cccgataact cccggttctg
     9241 cttcgcctgc ggggcggacc agacgggggg cggggacccg gccgccacgt ccgggcagat
     9301 ctcgagcctg atgacccgcc tccagcggat cgtcgagggc aagtacaagc tcgaacgtct
     9361 gctggggaag ggcgggatgg gagcggtgtt cctcgccaag gacctcacgc tcgaccggga
     9421 cgtcgccatc aaggtgctgc cccccgacgt ctcgcaggac gatcacgtgg tcaagcgctt
     9481 ccagcaggag gcgaagaccg cggccaagct cgaccacacc aacatcattc cgattttccg
     9541 ggtcgagagc gagggcgggc tcaactactt cgtgatgaag tacatctccg gcacgtccct
     9601 cgaggacctg ctggagcaga agaagccgtt gcccgtcgaa gagatccagc ggatcctctg
     9661 gcaggcgggc gtggcgctgg gccacgcgca ccaacgcggc atcgtgcacc gcgacgtgaa
     9721 gccggcgaac atcatgttcg accacgacgg caaggtgatg ctgacggact tcggcatctc
     9781 caaggccctc caggccgcgt caggcttcac gggcaccggc atgatcatcg gtacgccgca
     9841 ctacatggca cccgagcagg ccaagggcca gccggtggac ggccgggccg atcagtactc
     9901 gctgggcgtc gtggggtacc ggatgatcac cggtgcactg cccttcgccg gcgattcgat
     9961 tcacaccatt ctctacaagc acatcttcga ggaaccaccc ccggttcgct cggtgcggcc
    10021 ggatatcccc gagtttctgt ccaccgccat tcagcgggcg atgagcaagg aaccggcgca
    10081 gcggtacgcc acgatggagg acttcgcgac cgccgtcttc ccggagcagc cggtggcggc
    10141 gggccgctcg ggtggcccga tcacaccgaa gcccgtcgcg cgagcgccgg taaccgccga
    10201 gtcaccgacc gaagcgttca tctc
//
LOCUS       DASUBP010000284         4486 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_37235, whole genome shotgun sequence.
ACCESSION   DASUBP010000284 DASUBP010000000
VERSION     DASUBP010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37235"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VFU75_10825"
     CDS             complement(<1..653)
                     /locus_tag="VFU75_10825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467375.1"
                     /translation="MRSQFVYAGIVAIGLTAACQQATAPATQGQLKMAFSTRPTPAAP
                     TAGVRSSIVMGTDTLVSTSDTLVIDSVQIVLRKVELERVDGSVCDTVMSHEGDDGCEE
                     VEAGPVLVNLPLGDTAAQTFSVSIPVGSYEKIDFQVHAPTSDARDQAFLAANPLFAGT
                     SVRVVGTFNGKAFVYTTDLTAEQELELSPPLTVADTGAVALTIRTDVSKWFVNGTALV
                     "
     gene            complement(805..1704)
                     /locus_tag="VFU75_10830"
     CDS             complement(805..1704)
                     /locus_tag="VFU75_10830"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5467376.1"
                     /translation="MAHAADLNFTYSLIDRLFRLSMGEMADFSGAKFDGNFALTLEEA
                     QRAKHDFVAKSIGLKAESRVIDLGCGWGPFLNYARSRGAKGLGLTLSRGQYEACLRNG
                     LDVHLMDCRAVPRSAFGGFDAAVSLGAFEHFCSPDEFGAGKQEEVYGRFFRSVADLVP
                     DRGRLFLQTMVFGRNMIAPERIDLNAPRDSDEWILGLLRAQFPGSWLPMGRDQIERAA
                     APVFRLVAAENGRLDYIETIKRWRQRFAAFSFRKALVKLTLAPRYFLSRKFRDAFASG
                     VSANSVCFQRELLDHWRLVFEKV"
     gene            complement(1712..2011)
                     /locus_tag="VFU75_10835"
     CDS             complement(1712..2011)
                     /locus_tag="VFU75_10835"
                     /inference="COORDINATES: protein motif:HMM:NF013869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HEU5467377.1"
                     /translation="MTKEFITFLKEYGVIGLAIAVIIGGKLNDLVTSIVNGILMPIVS
                     FFVPGGAWRTATLDVGSIHFLIGPVAGALIDFLIVALIVFYLSKFVLREKEVHKK"
     gene            2095..2167
                     /locus_tag="VFU75_10840"
     tRNA            2095..2167
                     /locus_tag="VFU75_10840"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2129..2131,aa:Glu,seq:ctc)
     gene            2319..2390
                     /locus_tag="VFU75_10845"
     tRNA            2319..2390
                     /locus_tag="VFU75_10845"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2352..2354,aa:Glu,seq:ttc)
     gene            2487..3077
                     /locus_tag="VFU75_10850"
     CDS             2487..3077
                     /locus_tag="VFU75_10850"
                     /inference="COORDINATES: protein motif:HMM:NF013607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467378.1"
                     /translation="MTQTPALPHPARPILWRWAWNVRHLPDRLLHSLRRQHATRWLSD
                     TPAPANVLVVCQGNICRSPYGAYRLRELFARAGHACTIESAGFIGPDRPSPGNAIATA
                     ASRGIDLKPHRSQLLSKQLIDAAQLIIVMEARQADALRMVFGHVNAPILILGDLDPRH
                     VETRTVRDPALQSRAVFAESYDRIDRCLEVLVDHLA"
     gene            3074..3793
                     /locus_tag="VFU75_10855"
     CDS             3074..3793
                     /locus_tag="VFU75_10855"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEU5467379.1"
                     /translation="MSLTLWLGLAAGILLVHFFRERRLVGRFAAGATRGERNPSRAAL
                     ALATVIHERIPRGKDPAFLGLEIPALLGATPGAVLRRGGCCSGLSRLYVLALRSLGIR
                     AAQVTLYHESGLARHALVEVRIGTDRWIVDPTYGFHYVDRAGRPLGLDALRAGVEPTF
                     RPFIAGGSDGYPLNNYYAFDYRRSKTANWTMTLWRRMTYPTLRLLTAGGIDTLRQPGF
                     LEWPQLVVAGGVVAALICIWT"
     gene            3911..>4486
                     /locus_tag="VFU75_10860"
     CDS             3911..>4486
                     /locus_tag="VFU75_10860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467380.1"
                     /translation="MSTRLRLIQFPRVALGVAVACAACKVGSLVDSGPTDLGNATHLA
                     FVTQPASATAGAPIRVQVSAVDSSGGVVAVFNGRVTLALAANPGGDALHGTVTVTAVQ
                     GTATFTDIRIDRAASGYTISAATSGLTGATSGSFDVSAGSPVATTYMTQPTSTTSGSN
                     ITPAVQVQIVDAFGNPVTQYSGAVTITLAHDG"
BASE COUNT          764 a         1592 c         1352 g          778 t
ORIGIN      
        1 accagcgccg tcccgttgac gaaccacttc gagacgtcgg tgcggatggt cagcgccacc
       61 gccccggtat ccgccaccgt cagcggagga gacagctcga gctcctgctc cgcggtcagg
      121 tcggtcgtgt acacgaaggc cttgccgttg aaggtgccca ccacccggac gctggtcccg
      181 gcgaagagtg gattggccgc caggaacgcc tggtcacgcg catcggacgt cggagcgtgc
      241 acctggaaat cgatcttctc atagctcccc accgggatcg acacggagaa cgtttgtgcc
      301 gcggtatctc ccaggggcag attcaccagc accggacccg cttcgacttc ctcgcagcca
      361 tcatcccctt cgtgactcat gaccgtatcg cagacggaac cgtccacccg ctccagctcg
      421 accttccgca gcacgatctg gacggagtcg atcacgagcg tatcgctcgt gctcacgagg
      481 gtgtcggtgc ccatcacgat ggagctgcgg accccggcgg tgggcgccgc gggcgtcgga
      541 cgggtgctga aagccatctt gagttggccc tgcgtggccg gcgcggtggc ctgttggcag
      601 gccgccgtca ggccgatcgc cacgatgccg gcataaacga attgcgagcg cattgctgaa
      661 tctccttagg gttgaagtca cgcctcggcc ttccctaagg caggctccat gccgtcaccc
      721 gcctgcacct aactcattgc gtacgttcga cctgacatga acgggactgc gctgtggcgg
      781 ggcatctggc tcaaaacggt ccagttagac cttctcgaac accagcctcc aatggtcgag
      841 cagctcccgc tggaagcaga cgctgttggc gctcacccct gaagcgaagg cgtcgcggaa
      901 tttgcggctc aggaagtacc gcggcgccag agtcagcttc accaacgcct tccggaagga
      961 gaacgccgcg aaccgctgcc gccaccgctt gatcgtctcg atgtaatcga gccggccatt
     1021 ctccgcggcg accaggcgaa acacgggcgc cgcggcccgc tcgatctggt cccgccccat
     1081 cggcagccac gaccccggaa actgcgcccg gagcaatccc aagatccact cgtccgagtc
     1141 cctcggcgcg ttgaggtcga tccgctcggg cgcgatcatg ttgcggccga acaccatggt
     1201 ctgaagaaac aggcggcccc ggtcgggcac cagatcggcc accgaccgga agaatcggcc
     1261 gtagacctcc tcctgtttcc ccgccccgaa ctcgtcgggc gaacagaagt gctcgaacgc
     1321 tccgaggctg acggcggcgt cgaaccctcc gaatgccgac cgcggcaccg cccggcaatc
     1381 catgagatgg acgtcgagcc cgtttcgcag gcacgcttca tactgcccgc gggaaagggt
     1441 gaggccgagg cctttggcgc cccggctcct ggcatagtta aggaaaggcc cccacccgca
     1501 cccgaggtcg atgacccgcg actccgcctt gaggccgata ctcttggcca cgaagtcgtg
     1561 cttggcgcgc tgcgcctcct cgagggtcag cgcgaagttc ccgtcgaact tcgccccgga
     1621 gaagtcggcc atttcgccca tgctgaggcg aaagagccgg tcgatgagcg agtacgtgaa
     1681 gttgaggtca gccgcgtgtg ccattttgta cctacttctt gtgaacttct ttttccctga
     1741 gcacgaactt cgagagatag aacactatca gcgctacgat cagaaagtcg atcaacgcac
     1801 ccgcgaccgg cccgatcagg aaatggatgg agccgacgtc gagcgtcgcg gtcctccagg
     1861 cgccccccgg cacgaagaag ctcacgatcg gcatcaggat gccgttgacg atggaagtga
     1921 ccagatcgtt gagcttcccg ccgatgatga cggcgatcgc gagcccgatg acaccgtatt
     1981 ccttgaggaa cgtgatgaat tccttggtca tgttgccccc gctatataag aggaagggat
     2041 ggggccccga gcgcttgcgg cccctgctcc gtcctggcga tattgaccgc ccccggcggt
     2101 atcgtctagg ggactaggac ggagccctct caaggctcaa acacgggttc gaatcccgtt
     2161 accgctataa ctacccgaga aacgtcaagg aaaaacgtca aggaaaaacg tcaaggaaag
     2221 acgacctgtc acaccggact tttctcgacg tcgttcctcg acgtagttcc ttgacgcctc
     2281 tcctcgacgc cttatttctc gactgttttt gtagttctgc ccccatcgtc taacggttag
     2341 gacaccactc tttcaaggtg ggaacagggg ttcgattccc cttgggggca ctttcctgac
     2401 gaggcggccc tgcttccccc gcagcgtccg cgctccgctg ttgcagactc ccgtccctcg
     2461 gcccggttgt tgctccctca ccgggcatga ctcaaacacc cgcactccct caccccgccc
     2521 gccccatatt gtggcgctgg gcctggaacg ttcgccacct tcccgatcgc ctcctgcatt
     2581 cgttgcgccg ccagcacgcc acccgctggc tctccgacac ccccgcgccc gcgaacgtgc
     2641 tcgtcgtgtg tcaggggaac atctgccgca gcccctacgg cgcgtaccgg ctgcgggagc
     2701 ttttcgctcg agcaggtcac gcgtgtacga tcgaatcggc gggcttcatc ggacccgacc
     2761 ggccctcgcc gggaaacgcg atagcgaccg cggcgtcacg cggcatcgac ctcaaaccac
     2821 accgctccca actgctcagc aaacagctga tcgacgccgc gcagctcatc atcgtgatgg
     2881 aggcccgcca agccgacgcc ctccgcatgg tcttcggtca cgtgaacgca cccatcctca
     2941 tactgggaga cctcgatccc cgccacgtcg agacccgcac ggttcgcgac ccggcactgc
     3001 aatcccgcgc ggtctttgcc gagagctacg accgcatcga ccgctgtctg gaggtcctgg
     3061 tggaccatct cgcatgagcc tcacgctctg gctggggctc gccgcgggca tcctcctcgt
     3121 tcactttttc cgggagcgcc gactggtcgg ccggtttgcc gccggtgcca ctcggggtga
     3181 gcggaacccc agccgggccg ccctcgccct ggcgaccgtc attcacgagc gcattccccg
     3241 cgggaaggat cccgcgttcc tcggtctcga gatccccgcc ctgctcggtg cgacgcccgg
     3301 agcggtgctt cgccggggtg gctgctgctc gggactctcg cggctttacg tcctcgcgct
     3361 ccggtcgctc gggatccgcg cggcccaggt cactctctat cacgaatcgg gactcgcgcg
     3421 ccacgccctc gtcgaagtgc ggatcggcac cgaccgctgg atcgtggatc ccacctacgg
     3481 cttccactat gtcgaccgag ccggccggcc tctcggactc gacgcgttgc gcgccggtgt
     3541 cgaaccgacc ttccgcccct tcatcgccgg cggcagtgac ggctatccgc tgaacaacta
     3601 ctacgcgttc gactaccggc gctccaagac ggcgaactgg acgatgacgc tctggcggcg
     3661 gatgacctat cccaccctgc gcctgctcac ggccggcggg atcgataccc tccggcagcc
     3721 cgggttcctc gagtggccgc agctcgtcgt cgccggcggg gtcgtcgccg ccctgatctg
     3781 catttggact tgaagcgacg tcgccactgc cgcttcgagc acgcagcatc ccaggagcga
     3841 cgaccgaccc gcaccacctc gctcgcccgc cgttcatgcg ttggtccgcg ctttgcacct
     3901 tccgggggac atgagtactc gacttcggct catccaattc cctagggtcg cgctgggcgt
     3961 cgccgtggcc tgcgcggcgt gcaaggtcgg gtcgctggtc gacagcgggc caaccgacct
     4021 ggggaacgcg acccatctgg cattcgtcac gcagccggcg agcgcgacgg cgggcgctcc
     4081 gatccgcgtc caggtctccg cggtcgactc gagcggaggc gtggtggcgg tcttcaacgg
     4141 acgggtgacg ctcgcgctgg ccgcgaaccc cggtggagac gcgctgcacg ggacggtcac
     4201 ggtgaccgcg gtgcagggca ccgccacgtt cacggacatc cgcatcgacc gcgccgcctc
     4261 cggatacacc atctccgcgg cgacatcggg attgacgggt gccacgagcg ggagcttcga
     4321 cgtaagcgcg gggtctccgg tcgcgaccac ctacatgacc cagccgacgt ccactaccag
     4381 cgggtccaac atcacgcccg ccgtgcaggt ccagatcgtc gacgcgttcg gcaacccggt
     4441 gacgcagtac agcggggcgg tcaccatcac gctcgcgcac gacggc
//
LOCUS       DASUBP010000285        12934 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_242260, whole genome shotgun sequence.
ACCESSION   DASUBP010000285 DASUBP010000000
VERSION     DASUBP010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 12934)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12934)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12934
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242260"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="VFU75_10865"
     CDS             complement(<1..203)
                     /locus_tag="VFU75_10865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467381.1"
                     /translation="MIQLRVNTLTALPALQYMLADAGLTAPHFSFRAAWDVFQKYLGL
                     PSEAQDDIAGFQTTWVRENALDPV"
     gene            complement(254..748)
                     /locus_tag="VFU75_10870"
     CDS             complement(254..748)
                     /locus_tag="VFU75_10870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467382.1"
                     /translation="MRRLVLALGLAVLPAMVSAQGGADSTAQVKRIQEEIRLRWHQHI
                     VTQLQLNSDQASQLQATEDKYDGMRRPIQRRQVEIAEELNRQMQPGVAADNDAVARLL
                     AEREDNRAKLQDLDRQQDREMAGYLSPVQRVRYQRQREWLKGQIQQQRERRQEGRPPR
                     RRLR"
     gene            complement(748..1182)
                     /locus_tag="VFU75_10875"
     CDS             complement(748..1182)
                     /locus_tag="VFU75_10875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467383.1"
                     /translation="MIDDIRDEDLQDEELVAAARQLGVQAAGQLDMERTARGVVARWR
                     NERRPRLTPFWKSPAMLRVAAALVLLFAGIETYEHRHRLVQQVATVEATDAGLEGLSE
                     DQLQSILPAVEQTPEVETTAYDAGLEGLTPDELRSVLGAMGS"
     gene            complement(1179..1724)
                     /locus_tag="VFU75_10880"
     CDS             complement(1179..1724)
                     /locus_tag="VFU75_10880"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEU5467384.1"
                     /translation="MASPQSAAATDADLIARWQGADERAATELVLRHGAPLARFLSAS
                     GAQPSDLDDLVQETFLRAFRSIAGFRGQCQFRTWLLTIGSNVLKDEGRRAGKRKVVPL
                     DESLRASGGSPHDDAVASEAEARLTGALQELPRLQREVFLLRAQQGMEYEEIAPALGT
                     TPGAARVHYHHAVKRLKERLQ"
     gene            1806..2399
                     /locus_tag="VFU75_10885"
     CDS             1806..2399
                     /locus_tag="VFU75_10885"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEU5467385.1"
                     /translation="MRPYDERLEHLLALAAKVFAEAGYHSTSMRDLAAASGMSLAGMY
                     YYVRSKEDLLALIQERCFTGVVDGARKALDGHSDPQDRLEAFIRHHVTFFTAHMSEMK
                     VLSHESSVLSGDLRRKVNHIKRRYVDLLEGLLKDAAPDERPVDRSAAAYALFGMMNWI
                     YNWYDPAGEIDPERLAGLIARIFLGGFVEARSTVHGG"
     gene            2417..3229
                     /locus_tag="VFU75_10890"
     CDS             2417..3229
                     /locus_tag="VFU75_10890"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEU5467386.1"
                     /translation="MTKPTPETKSADLVLYSVADGIATIELNDPPANTYTHEMMRELD
                     DAILQARFDDSVHVILLRGHGDKFFSAGANIGMLNKVTPRFKYFFCLHANETLNRLEH
                     TPKLTIAALNGHTVGGGLEIAMACDIRLAKENAGKIGLPEVNLGVLPGTGGTQRLGRM
                     VGKQMAIEMMVKGETFSFDKAKEVGLVNEVFPADGYWEQVMLYAKQFCPPHKASKAVG
                     LIKRSVQSGLEVPFESALAIERELQQQLFQSDDAKEGIAAYNEKRVAEFRGK"
     gene            3226..4665
                     /locus_tag="VFU75_10895"
     CDS             3226..4665
                     /locus_tag="VFU75_10895"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5467387.1"
                     /translation="MKTDLFINNEWRPALSGKRFPVENPATEEIIAEVAQGDAADADH
                     AVKAARACFESDAWRGMSARKRGRLLFKTADLLEARLEEFARLETAHNGKTLFESKIE
                     ISMTVNTLRYYAGWADKITGETLPVEGPFFTYTLREPIGVVGAIVPWNFPLNIASWKF
                     APALAAGCTMILKPASETPLTALLFAELIAEAGFPPGAFNVLPGGGSTAGAALVRHPD
                     VDKISFTGSTEVGRQIMKMAADTNKRITLELGGKSPNVIFADADLKAAVRGAQTGIFY
                     GKGEVCAAGSRLLVEKSVHDQVVEQLAEGAKKLTPGDPLDKNTRMGALVSRKQQETVL
                     GYVKKGLDEGATLVAGGKAVKPNGKGYFVEATVFDKVQPEMTIAHEEIFGPVLAVMSF
                     DDYDSGVALANKTIYGLAAGIWTKDVGKAHRAAKAIRAGTVWINSYNFYDSGAPFGGF
                     KASGFGRDLGVDALDGYLETKTVWTSLGG"
     gene            4682..5851
                     /locus_tag="VFU75_10900"
     CDS             4682..5851
                     /locus_tag="VFU75_10900"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HEU5467388.1"
                     /translation="MAKDAYVEIALRTPVGKHGGSLAGVRPDDLAAVPIRAVVERSGV
                     DPKQIDDVILGCANQAGEDNRNVARMALLLAGLPVEVPGQTVNRLCGSGLQAVVAASH
                     AIKSGEGERFIAGGVESMTRAPTVMLKSAEPWSRKAPEIADTTVGWRFVNPKLKPDWT
                     ISLGETAEKVAEQYGITRGEQDAFSAESQRRAQEAQKACVFTDELVPVALADGSSFSK
                     DEYPRAGTTAEKLATLKPAFKKNGTVTAGSSSGINDGAAAIFVTSAPKQPLARIVASA
                     VAGVDPSVMGLGPIPATRKALARAGLKANQLDLVELNEAFAAQAIACIRDLSLDPAKV
                     NIYGGGIALGHPLGCTGAKILATLAHALKRTGGRYGLVTMCIGVGQGIAVIVERV"
     gene            5851..6591
                     /locus_tag="VFU75_10905"
     CDS             5851..6591
                     /locus_tag="VFU75_10905"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HEU5467389.1"
                     /translation="MAAFEKVLVEIAEGVATLTLHRPDKLNALDVELCQDLIAALETV
                     SGSSQVRAIVITGAGRGFCAGADLKVLGPQGNQLVEAGVRIAQLVRNAPQPVIAAVNG
                     AAAGGGANLALACDYRIASDQAKIGQVFHKLGLGVDWGGSFFLPRTVGTARALELVWS
                     ARMVGAPEALALGLFQEVVPAERLLQRAGELAKLWASQPSEAVRRTKIGLYASEGRTL
                     AQMLDWEMTTQRELFVTPDARERIARSL"
     gene            6605..7723
                     /locus_tag="VFU75_10910"
     CDS             6605..7723
                     /locus_tag="VFU75_10910"
                     /inference="COORDINATES: protein motif:HMM:NF016993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Phenylacetic acid catabolic protein"
                     /protein_id="HEU5467390.1"
                     /translation="MVRKIGNFDDWKDYFRYWQSSIGLPEGELRNFTFDAKFGDPEVP
                     HIEFGHYKGQRKWPTVMHIPDQRIRDALLNLIVYQGDTEFASVEQQRNLLTHAPSDYD
                     LLSLMRVMTEEMRHGWQMSYLLCTHFGDEGRREAAKLLERRADEGERLLGSFNEIVNN
                     WLDFFTYTQFIDRDGKFQLKMLSYSAFDPLARSMGPMLKEESFHLGTGNNGLLRIVKA
                     GKMPPEVIQRFFYKWIPTAFDLFGTDHSSSAHWAYVWGLKGRYDEREHQSEPDKAQLN
                     ELSRELYRQEIAKLVERLNMFIPEKERQLKVPSLKFNRRIGMYARQYWTTDGEPIDAE
                     KYQAYLASVLPRMEDIQFVKDLEKEPNWIEPKKADSLA"
     gene            7720..8424
                     /locus_tag="VFU75_10915"
     CDS             7720..8424
                     /locus_tag="VFU75_10915"
                     /inference="COORDINATES: protein motif:HMM:NF015000.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEU5467391.1"
                     /translation="MSPAAGVDQPLVIAHRGASGAEYENSLAAFRKAGELGADGIELD
                     IHATTDGALIVHHDPAIGGTVIARATAADISKLKLPNGEPVPTLAQAFVAAGPKLRVY
                     VEVKTLPPQWDGQLFAAIDAGPNPAGYAVHAFDHRIIQRLRRQRPKLQAGVLSASYLD
                     DNVAPVRAAGAQMLWQDQAMIDAALVEEVHDAGFGVIAWTPNEISDLSRLASQGVNGL
                     CTNYPDRARRVVDGLK"
     gene            8421..9290
                     /locus_tag="VFU75_10920"
     CDS             8421..9290
                     /locus_tag="VFU75_10920"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HEU5467392.1"
                     /translation="MTAPDTSWEVTVVGAGTMGHGIAYVSAFAGCRVTLTDLRADALE
                     GARTKIEALLAGGLKRGKLTEDDRAGVHERLHFEPALAAAVRHADVVIEAVVEQMPVK
                     QRIFAEVEREAPDGALLATNTSSLSVGGIAAALKEPWRLLGMHFFNPVHLMKLVEIVT
                     HGRTGAPQVERATALARVLGKEPILVKDSPGFASSRLGVVLGLEAMRMLEQGVASAAD
                     IDKAMELGYNHPMGPLKLTDLVGLDVRLAIANYLHEELKLPHYAPPKILKDKVAKGEL
                     GKKSGQGFYPWTD"
     gene            9278..10153
                     /locus_tag="VFU75_10925"
     CDS             9278..10153
                     /locus_tag="VFU75_10925"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5467393.1"
                     /translation="MDRLTRITDVHVHIQPWRDLKPAVQDAMRKGKEDHWAFLLSMMD
                     DPKVLLDVMDRSGVWRIGMVNYTSPDVMGFTETTNTFIAKYAQANPERLMPYGGVHAR
                     FTKDPMGDVDQLVELGIRCLKIHPPHQLFTANAYTEGLDALAGIYRRCEERGLPVMVH
                     TGTSIFPGARSKYGNPMELDDVAVDFPDLRIVMAHGGRPLYMEEAFFVLRRHKNVWLD
                     VSGIPPAKLLEYFPRLGEISGKTVWGTDWPSPGVKDLKQNIDQFLGLSLPDDQKRAIL
                     ETNPLALFPAVPQRT"
     gene            10195..11577
                     /locus_tag="VFU75_10930"
     CDS             10195..11577
                     /locus_tag="VFU75_10930"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU5467394.1"
                     /translation="MRVARMSLALAVGLVSAAPGATVTAQTAQQVVGAVAIDSFRLAN
                     GLQVLYAEQHETPVVTVDLWYRVGARNERPKVSGFAHLFEHMMFQGSAHVGKAEHFKY
                     VERAGGDENGSTHDDYTNYYETLPSNRLNLALWLEADRMRSLAVTQENLENQRQAVEE
                     EKRLRVDNQPYQGALRGSLIWPYDSTNCFAYAHPGIGVMEDLDSAKLEEVRAFHDTYY
                     APNNVTLVVAGDFKPAELKQLVTSYFSDIPSKPAPAAVTCNYKLSPGIVQRAVADSHA
                     TLPAVLRAYRIPAAADEDTPALDLLNSILGQGESSRLNVEVVRKDTAALTASSFSVNE
                     RRGPGTLLVLGIANRGVTAGRLDSLLASQVEAIRSNGVTAGELARAKNQYRAGVIQSR
                     ETTFGVAEGINYYSLLFPSVSDMNTDPQRYMAVTADDIKRVAQKYLDPANATVVTVNP
                     AGKSQSGGDQ"
     gene            11574..>12934
                     /locus_tag="VFU75_10935"
     CDS             11574..>12934
                     /locus_tag="VFU75_10935"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HEU5467395.1"
                     /translation="MSRRAFVFALAASAATSSLAVAQTPDSFPPHAPSPATLAPVRFP
                     PFTETTLPNGMTLLVVENHSQPMLSLSLSFRAGSIHDPVGKEGLAELTAQILTKGSAK
                     HTGDQIAAMIEGVGGGLSASAGSDFFSISENVLSDHADVALGLLGEVSRTPALSADEL
                     ELARTQALSALQLELSQPSSIAARFFRQTIYGKNPYGRETSEASYKAITRDDVTAFAK
                     RYLVPSGALLVMAGDVTPAKAKAWATRIFGTWRGTPPPSTPPMAAAAPPTQILLINRP
                     GSVQANIILGNTTYGPLDSSYYATRVAMQVLGGGADARLFLILREAKGWTYGAYAGMN
                     RYRGLGFWQATFEGRTAVADSALAEMLHQVDRLRTEPIPENELAAAKGFLVGSFPLTI
                     ETSDQVAGAVGNARLLGLGNHYVQTYREHLNAITSAQAEAAAAKALKRDHLTIVVVGD
                     AQAL"
BASE COUNT         2279 a         4431 c         4146 g         2078 t
ORIGIN      
        1 accggatcga gcgcgttctc ccggacccag gtggtctgga atcccgcgat gtcgtcctgg
       61 gcctcagagg ggaggccgag gtacttctgg aagacgtccc acgcggcccg aaacgaaaaa
      121 tgcggggccg taagccccgc atcggccagc atgtactgga gcgcggggag ggcggtgagc
      181 gtgttcactc ggagttggat catggtcctg tcaaattagc tccaacccgt cctacccgct
      241 tgtaccggtt ctgttaccgc agcctccgcc gggggggcct gccctcctga cgccgctccc
      301 gctgctgctg gatctgtccc ttcagccact cccgctgccg ctgataacgg acccgctgca
      361 cggggctcag gtaccccgcc atctcccggt cctgctggcg gtccagatcc tgcagcttcg
      421 cccggttgtc ctcccgttcc gccaggagcc gggcgaccgc gtcgttgtcg gccgcgacac
      481 caggctgcat ctgccggttc agttcttccg cgatctctac ctgccgccgc tgaatcggcc
      541 ggcgcatccc gtcatacttg tcctcggtcg cctgcaactg cgacgcctga tccgaattca
      601 gctggagctg cgtcacgata tgctggtgcc accgcagccg gatctcctcc tgaatgcgct
      661 tgacctgggc cgtactgtcc gccccgccct gcgcgctgac catggccggc agcaccgcca
      721 acccgagtgc cagaacaagt cgccgcatca gctccccatc gcgcccagca cggaccgcaa
      781 ttcgtccggc gtgagcccct ccaacccggc gtcatatgcc gtggtttcga cttcaggtgt
      841 ctgctccacc gcgggcagaa tggactgcaa ttggtcttcg gacaacccct cgagtcccgc
      901 gtccgtggct tcgaccgtcg ccacctgctg caccaggcga tgccggtgct catacgtttc
      961 gatcccggca aagagcagca cgagcgcggc cgccacgcgc agcatggcgg gcgacttcca
     1021 aaacggcgtc agccggggcc gccgctcgtt ccgccaccgg gcgacgacgc cgcgtgcggt
     1081 ccgttccatg tcgagttggc cggcagcctg gacgccaagc tgccgcgccg cggcgaccag
     1141 ctcctcatcc tgcaggtctt catcccggat gtcatcgatc attgcagacg ctccttcaac
     1201 cgcttgaccg cgtgatgata atgcacacgg gccgctcccg gagtcgtgcc caaggccggc
     1261 gcgatctcct cgtactccat tccctgctgc gcccgcagca gaaagacttc ccgctgcaat
     1321 cggggcagct cctgcaacgc ccccgtcagc ctcgcttctg cctccgacgc caccgcgtca
     1381 tcgtgtggag acccccccga tgcccgcagt gactcgtcga gtggcaccac tttccgcttc
     1441 ccggccctgc gcccctcatc cttcaacacg ttgctcccga tggtcaggag ccacgtgcga
     1501 aactgacatt gcccccggaa tcccgcgatc gaccggaagg cccgcaggaa cgtctcctgc
     1561 accaggtcgt ccaggtccga cggctgggcc ccgctcgcgg agaggaaccg cgccaagggc
     1621 gccccatgcc gcagcaccag ctcggtagcc gcgcgttcgt ccgctccctg ccagcgggcg
     1681 atcaggtccg catcggtggc ggcagccgac tggggagaag ccattggggt tgtgtcagtg
     1741 gacgcgggag ttgcgggaat attaagcccc ggctagtttg attttcgaac gatcgttcga
     1801 accctatgcg cccctacgac gagcggttag agcacctctt ggccctggcg gccaaagtct
     1861 tcgccgaggc gggctaccac tccacctcca tgcgggacct ggccgccgcc agcgggatgt
     1921 ccctcgcggg catgtactac tatgtgcggt ccaaggaaga tctcctggcc ctgatccagg
     1981 agcggtgctt taccggtgta gtcgacggcg cccggaaagc cctcgacggg cattcagacc
     2041 cccaagaccg gctggaggcc ttcattcgtc accacgtcac cttcttcacg gcccacatgt
     2101 ccgaaatgaa ggtactctcg cacgaatcca gtgtgctctc gggtgacctg cgtcgcaagg
     2161 tgaaccatat caagcgccgg tacgttgatc tgctcgaagg cctgctcaag gatgccgccc
     2221 cggacgagcg cccggtggac cggagcgcgg cggcgtacgc cctctttggc atgatgaact
     2281 ggatctacaa ctggtacgac cccgcgggtg aaatcgaccc cgaacggctc gcgggcctga
     2341 tcgcgcgaat cttcctcggc ggtttcgtcg aggcgcgctc cacggtacac ggaggctaga
     2401 ggcggttatg gcgacgatga cgaaacccac ccccgaaacg aagtcggccg atctcgtgct
     2461 ctacagtgtg gcggacggca tcgccaccat cgagctgaac gatccgccgg ccaataccta
     2521 cacgcacgag atgatgcgcg agctcgatga cgcaatcctc caggcgcgct tcgacgactc
     2581 ggtccacgtg atcctgctcc gcgggcacgg cgacaagttc ttctccgccg gcgccaacat
     2641 cgggatgctg aacaaggtca ccccccgctt caagtacttc ttctgcctgc acgccaacga
     2701 gaccctcaat cgcctcgagc acacccccaa gctcaccatc gcggccctga acgggcacac
     2761 ggtgggcggc gggctcgaga tcgcgatggc ctgcgacatt cgtctcgcca aggagaacgc
     2821 cggcaagatc ggcctccccg aagtgaacct cggcgtcctg ccggggaccg gcggcacgca
     2881 gcgcctcggc cggatggtcg ggaagcagat ggcgatcgag atgatggtga agggtgagac
     2941 gttctcgttc gacaaggcga aggaagtcgg cctggtgaac gaggtcttcc ccgccgacgg
     3001 ctactgggag caggtgatgc tgtacgcgaa gcagttctgc cccccgcaca aggcgtccaa
     3061 ggcggtcgga ctcatcaagc gctcggtgca gtcgggcctc gaagtgccct tcgagtccgc
     3121 actcgccatt gagcgagagc tccagcagca gctcttccag tcggacgacg cgaaggaagg
     3181 catcgcggcc tacaacgaga agcgcgtggc ggagttccgc gggaagtgaa aaccgatctc
     3241 ttcatcaaca acgagtggcg ccccgccctc tccgggaagc ggttcccggt ggagaatccc
     3301 gccaccgagg agatcatcgc cgaggtcgcg cagggcgacg cggccgatgc cgaccatgcg
     3361 gtgaaggcgg cgcgggcctg cttcgagtcc gatgcctggc gggggatgtc ggcgcggaag
     3421 cgcgggcggc tgctcttcaa gaccgccgac ctgctcgaag cgcgcctcga ggagttcgcc
     3481 cggctcgaga cggcgcacaa cggcaagacg ctcttcgagt cgaagatcga gatctcgatg
     3541 accgtcaaca ccctgcgcta ctacgcgggg tgggccgaca agatcacggg cgagacgctg
     3601 ccggtcgaag ggcccttctt cacctacacg ctgcgcgagc ccatcggcgt ggtcggcgcc
     3661 atcgttccct ggaatttccc gctcaacatc gcaagctgga agttcgcgcc ggcgctggcg
     3721 gcgggatgca ccatgatcct gaaacccgcg tccgaaaccc cgttgacggc cctgctcttc
     3781 gcggaattga tcgccgaagc gggattcccg ccgggggcgt tcaacgtcct ccccggtggc
     3841 gggtcgacgg cgggcgcggc attggtccgt caccccgacg tcgacaagat cagcttcacc
     3901 ggttcgaccg aggtcgggcg ccagatcatg aaaatggccg ccgacacgaa caagcggatc
     3961 acgctcgagc tcggcggcaa gagtccgaac gtgatcttcg ccgacgccga cctcaaggcc
     4021 gcggtccgcg gcgcccagac cgggattttt tacggcaaag gtgaagtctg cgccgcgggc
     4081 tcgagactcc tggtcgaaaa gagcgtgcat gaccaggtgg tcgagcagct cgccgaaggg
     4141 gcgaagaaac tcacccccgg cgatcccctc gacaagaaca cgcgtatggg cgcactcgtc
     4201 tccagaaaac agcaggagac ggtactcgga tacgtgaaga agggcctgga tgagggagcg
     4261 acgctcgtgg cgggcggcaa ggccgtgaag ccgaacggca agggctactt cgtcgaggcg
     4321 accgtgttcg acaaggtcca gcccgagatg acgatcgcgc acgaagagat cttcggcccg
     4381 gtcctcgcgg tcatgtcgtt cgacgactat gactcggggg tcgccctggc caacaagacc
     4441 atctacggac tcgcggccgg catctggacg aaggatgtgg gcaaggcgca ccgtgcggcg
     4501 aaggcgatcc gcgcgggcac cgtctggatc aactcataca acttctacga ctccggcgcg
     4561 cccttcggcg ggttcaaggc ctccgggttc ggccgcgacc tcggcgtgga tgccctggac
     4621 gggtatctgg aaaccaagac cgtctggacg tctctgggtg gctaagaggc taaggggcta
     4681 agtggccaag gacgcttacg tcgaaattgc gttgcgtact cccgtgggca agcacggggg
     4741 ctcgttggcg ggagtgcgcc ccgatgatct ggccgccgtt cccatcagag ccgtcgtcga
     4801 gcgctccggg gtggatccga agcagatcga cgacgtcatc ctcggctgcg ccaatcaggc
     4861 cggcgaggac aaccgcaacg tggcccgcat ggcgctgctc ctcgccggac tccccgtgga
     4921 ggtgccgggc cagacggtga accggctttg cggatcgggc ctccaggccg tagtggccgc
     4981 ctcgcacgcg atcaagtcgg gcgaagggga gcgattcatc gccggcggcg tcgagtccat
     5041 gacgcgtgcc ccgaccgtga tgctcaagtc cgccgagccc tggagccgca aggcgccgga
     5101 aatcgcggac accaccgtcg ggtggcgctt cgtgaatccg aaactcaagc cggattggac
     5161 catctccctc ggcgagaccg ccgagaaggt ggcggagcag tacggaatca cgcggggcga
     5221 gcaggatgcg ttttcggccg agagccagcg ccgcgcgcag gaagcgcaga aggcctgcgt
     5281 gttcaccgac gagctggtcc cggtggcgct cgccgacggc tcgtccttct ccaaggacga
     5341 atatccccgg gccgggacga ccgccgagaa gctcgcgacg ctgaagccag ccttcaagaa
     5401 gaacggaacc gtcaccgcgg gctcgtcgag cggaatcaac gacggcgccg cggcgatctt
     5461 cgtcacgtcc gcgccgaagc agcccctcgc ccgaatcgtc gccagcgccg tggcgggtgt
     5521 cgatccgagc gtcatggggc tgggtccgat ccccgcgacc cggaaggccc tcgctcgagc
     5581 cggcctcaag gccaatcagc tggatctggt ggagctgaac gaggcgttcg cggcacaggc
     5641 catcgcctgc atccgcgacc tctccctcga ccccgccaag gtcaatatct acggcggcgg
     5701 catcgcactc ggccacccgc tcggttgcac cggcgccaag atcctcgcga ccctggccca
     5761 cgctctgaag cgcaccgggg gccgctatgg cctcgtgacg atgtgcatcg gggtgggaca
     5821 agggatcgcc gtcatcgtgg agcgcgtgta gtggcggcgt tcgagaaagt gctcgtcgag
     5881 atcgcggagg gggtcgcgac tctcaccctg caccgccccg acaagctgaa tgcgctggac
     5941 gtggagctct gccaggatct catcgcggcc ctggagacgg tctcgggatc gagtcaggtc
     6001 cgcgcgatcg tcatcacggg cgcggggcgc gggttctgcg cgggcgcgga cctgaaggtc
     6061 ctgggccccc agggcaacca actggtggaa gctggggtcc ggatcgccca gctcgtccgg
     6121 aacgcgccgc agccggtgat cgcggccgtg aatggcgccg ccgccggcgg tggagcgaac
     6181 ctcgcgctcg cctgcgatta ccggatcgca tcggatcaag cgaagatcgg tcaggtattc
     6241 cacaagttgg gactcggggt cgactggggc gggagttttt tcctgccccg caccgtcggg
     6301 accgcccggg ccctggagct ggtctggagc gcccgcatgg tcggcgcgcc ggaagcgctg
     6361 gccctcggac tctttcagga ggtggtcccc gcggagcgcc tcctgcaacg ggccggcgag
     6421 ctggcgaagc tgtgggccag tcagccttcc gaagccgtgc gacggaccaa gattggcctg
     6481 tatgcgagcg aagggcgtac gctggcgcag atgctcgatt gggaaatgac tacgcaacga
     6541 gagttgttcg taacgccgga cgcgcgcgag cgcatcgccc ggtcactcta ggaggaatgc
     6601 tctgatggtt cggaagatcg gcaatttcga cgactggaag gactacttcc gctactggca
     6661 gagctcgatc ggcttgccgg agggtgagct ccgcaacttc accttcgacg cgaagttcgg
     6721 cgaccccgag gtgccgcaca tcgagttcgg gcattacaaa ggccaacgca aatggcccac
     6781 ggtgatgcac atccccgacc agcgcatccg cgacgctctg ctcaacctca tcgtctacca
     6841 gggggacacc gagttcgcgt cggtggaaca gcaacgcaac ctgctgaccc acgcgccgtc
     6901 ggactacgac ctgctctcct tgatgcgggt gatgaccgag gaaatgcgcc acgggtggca
     6961 gatgtcctac ctgctgtgca cccacttcgg tgacgagggc cggcgggagg ccgccaagct
     7021 gctcgagcgt cgggccgacg aaggcgagcg gctcctcggc tccttcaacg agatcgtgaa
     7081 caactggctg gatttcttca cctacacgca gttcatcgac cgggacggca agttccagct
     7141 caagatgctg tcctattcgg ccttcgatcc cctggcgcgc tcgatggggc cgatgctgaa
     7201 ggaagaatcc ttccacctgg gcacgggcaa caacggactg ctccggatcg tgaaggccgg
     7261 caagatgcct cccgaggtga tccagcgctt cttctataag tggatcccga cggcgttcga
     7321 cctgttcggc accgaccact cgtcctcggc gcactgggcg tacgtctggg ggctcaaggg
     7381 ccgctacgac gaacgcgagc accagagcga gcccgacaag gcccagctga acgagctctc
     7441 ccgcgagctg tatcgccagg agatcgcaaa gctggtcgag cgcctcaaca tgttcatccc
     7501 ggagaaggag cggcagctga aggtgccgtc cctcaagttc aaccgccgga tcggcatgta
     7561 cgcccggcaa tactggacca cggacggtga gccgatcgac gccgagaagt accaggccta
     7621 tctcgcatca gtgctgccgc ggatggagga catccagttc gtgaaggacc tcgagaaaga
     7681 accgaactgg atcgagccca agaaagccga ctcgctggcg tgagcccggc cgccggcgtc
     7741 gaccagccgc tcgtcatcgc gcaccggggc gcttcaggcg cggaatacga gaactccctt
     7801 gccgccttcc ggaaggccgg ggagctgggc gcggacggta tcgagctcga catccacgcc
     7861 accaccgacg gcgctctgat cgtgcaccat gaccccgcca tcggcggcac ggtcatcgca
     7921 cgcgcgacgg cggccgacat ctccaagctc aagctgccga acggcgagcc ggtgccgacg
     7981 ctggcgcagg ccttcgtggc cgcggggccg aagctccgcg tctatgtcga ggtgaaaacc
     8041 ctcccgcctc agtgggacgg acagctcttc gccgccatcg acgcgggtcc caatcccgcc
     8101 ggatatgccg tccacgcgtt cgaccaccgc atcatccagc ggctccgccg acagcgcccg
     8161 aagctgcagg ccggcgtgct ctcggcatcg tacctggacg acaacgtggc cccggtgcgc
     8221 gcggcgggtg cgcagatgct ctggcaggac caggcgatga tcgacgccgc tctcgtggag
     8281 gaagtccacg acgcgggatt cggcgtcatc gcctggaccc cgaacgaaat ctccgatctc
     8341 tcccggctcg cctcccaggg cgtgaacggt ctctgcacca actacccgga tcgcgcccgg
     8401 cgcgtggtcg acgggctcaa gtgacggcac ccgacaccag ctgggaagtg acggtagtgg
     8461 gggctgggac catgggccac ggcatcgcct acgtctcggc gttcgccgga tgccgggtga
     8521 cgttgaccga ccttcgcgcc gacgcgctcg agggggcgcg gaccaagatc gaagcgctcc
     8581 tggccggcgg gttgaagcgg ggcaaactca cggaagacga ccgggccggc gttcacgaac
     8641 gcctgcattt cgagcccgcc ctcgccgccg ccgtgcgcca cgccgacgtg gtgatcgaag
     8701 cggtggtcga gcagatgccg gtcaagcagc ggatcttcgc cgaggtcgag cgcgaagcgc
     8761 ccgacggtgc cctgctcgcc acgaacacct cttccctgtc cgtagggggg atcgccgccg
     8821 cgctcaagga gccctggcgg ctcctcggga tgcacttctt caatccggtg cacctgatga
     8881 agctcgtgga gatcgtgacg catggccgca ccggcgcgcc ccaggtcgag cgcgccaccg
     8941 cgctggcccg tgtgctcggc aaggaaccga ttctcgtgaa ggattcgccg ggattcgcgt
     9001 cgagccgcct gggcgtggtg ctgggactcg aggcgatgcg catgctcgag cagggcgtcg
     9061 cttccgcggc cgacatcgac aaggccatgg agctgggcta caaccatccc atgggaccgc
     9121 tcaagctcac cgacctggta gggctcgacg tccggctggc gatcgcgaac tacctccatg
     9181 aagagctgaa gctcccccac tacgcgccgc ccaagatcct gaaggacaag gtggccaagg
     9241 gggagctggg caagaagtcc ggccaggggt tctatccgtg gaccgactga cccgcatcac
     9301 cgacgtccac gttcacatcc agccgtggcg cgacctcaag cccgcggtgc aggacgcgat
     9361 gcggaaaggc aaagaggatc actgggcctt cttgctctcg atgatggacg atcccaaggt
     9421 cctgctcgac gtgatggacc ggtcgggcgt ctggcggatc ggcatggtga attacaccag
     9481 ccccgacgtg atgggattca ccgagaccac gaacaccttc atcgcgaaat acgcgcaggc
     9541 gaaccccgag cgcctcatgc cctacggagg cgtgcacgcg cggttcacca aagaccccat
     9601 gggggacgtg gaccagcttg tggagctggg aatccgctgc ctcaagatcc atccaccgca
     9661 tcagctcttc acggccaacg cgtacaccga gggtctcgac gcgctggccg gcatctaccg
     9721 gcgctgcgag gagcgtgggt tgccggtgat ggtccacacc gggacgagca tcttcccggg
     9781 ggcgcggtcc aagtacggca acccgatgga gctggacgac gtcgcggtcg acttccccga
     9841 tctgaggatc gtgatggccc acggtgggcg cccgctgtac atggaagagg cattcttcgt
     9901 cctgcgtcgt cataagaatg tgtggttgga cgtttccggg attccgcccg cgaaactcct
     9961 ggagtacttt ccgcgtctcg gggagatttc cggcaagacc gtgtggggca ccgactggcc
    10021 cagcccgggc gtgaaagacc tgaaacagaa catcgaccag tttctcggct tgtcccttcc
    10081 ggacgaccag aaacgggcga ttctcgagac caacccgctg gcgctgtttc ccgccgtccc
    10141 ccagcggacc tgagcagtac ccaacagcct tttctttcga gggagcgcgc tccaatgcgt
    10201 gtcgcacgga tgtcccttgc cctggccgtc ggcctcgtct ccgccgcgcc cggcgcgacc
    10261 gtcacggccc agaccgccca gcaggtcgtc ggcgccgtcg ccatcgattc cttccgcctc
    10321 gccaacggtc tccaggtgct ctatgcggag cagcatgaga caccggtggt cacggtggac
    10381 ctctggtacc gcgtcggcgc ccgcaacgag cgccccaagg tatcggggtt cgcccacctc
    10441 ttcgagcaca tgatgtttca agggtcggcg cacgtcggga aggccgagca tttcaagtac
    10501 gtggagcgcg ccggcggtga cgagaatggc tctacccacg acgactacac gaactattac
    10561 gagacgctgc cctccaaccg cctcaacctg gcgctctggc tcgaggcgga ccggatgcgc
    10621 tccctcgccg tgacccagga gaacctcgag aaccagcggc aggcggtgga agaggagaag
    10681 cggctgcggg tggacaacca gccctaccag ggcgccctgc gcggctccct catctggccg
    10741 tacgacagca ccaactgctt tgcctacgcc caccccggca tcggcgtcat ggaagatctg
    10801 gactccgcga agctcgagga ggtccgcgct ttccacgata cctactacgc tccgaacaac
    10861 gtcaccctgg tcgtggcggg tgacttcaag cccgctgagc tgaagcagct cgtcacgtcg
    10921 tacttctcgg acatccccag caagccggcg ccggctgccg tcacctgcaa ctacaaactc
    10981 tcccccggga tcgtgcagcg cgccgtggcc gattcccacg cgactctgcc cgccgtgctc
    11041 cgcgcctacc ggattcccgc ggccgccgac gaggacaccc ccgcgctcga tctcctgaac
    11101 agcatcctcg gtcagggtga gagctcgcgg ctgaacgtcg aagtggtccg gaaggacact
    11161 gcggcgctga ccgcgtcgag cttcagcgtg aacgaacgcc gcggaccggg cacgctgctc
    11221 gtcctcggca tcgccaaccg cggcgtgacc gcgggccgcc tcgattcgct gctcgcgtca
    11281 caggtcgaag ccatccggtc caacggcgtg accgccggcg agctggcccg cgccaagaac
    11341 cagtaccgcg ccggagtcat tcagtcgcgg gagacgactt tcggcgtggc cgaaggcatc
    11401 aactactaca gcctgctctt cccgagcgtg tccgacatga ataccgaccc acagcgctat
    11461 atggcggtga ccgccgacga catcaagcgc gtcgcccaga agtatctcga tcccgccaac
    11521 gccaccgtgg tgaccgtcaa cccggccggg aagtcgcagt cgggaggtga ccaatgagcc
    11581 ggcgcgcgtt cgtcttcgca ctcgcggcct cagcggcgac gtcatcgctc gccgtcgccc
    11641 agaccccgga ttcgtttccg ccccatgcgc cgtcgccggc caccctggcc cccgtgcggt
    11701 ttccgccctt cacggagacc acgcttccga acgggatgac gctcctcgtc gtcgaaaatc
    11761 actcgcagcc catgctctcc ctgtcgctgt cgttccgagc gggatcgatt cacgatcccg
    11821 tcgggaagga agggctggcc gagctgaccg cgcagatcct gaccaagggt tcggcgaagc
    11881 acaccggcga ccagatcgcc gcgatgatcg agggagtggg tggtggcctg tcggcctcgg
    11941 ccgggtcgga tttcttctcg atctccgaga acgtcctctc cgaccacgcg gatgtcgcgc
    12001 tcggcctgct tggcgaagtg agccggactc ccgccctctc ggcagatgag ctcgagctgg
    12061 cccgcaccca ggcgctctcg gcgctgcagc tcgagctctc gcagcccagc tccatcgccg
    12121 cgcggttctt ccgccagacc atctacggca agaaccccta cggccgtgag accagcgagg
    12181 cgtcctacaa ggccatcacg cgcgatgacg tcacggcgtt cgccaaacgc tatctcgttc
    12241 cttcaggcgc cctcctggtg atggcgggcg atgtgacgcc cgccaaggcc aaagcctggg
    12301 cgacccggat cttcgggacc tggcggggta cgccgcctcc gtcgacccct ccgatggctg
    12361 ccgctgctcc gccgacccag atcctgctga tcaaccggcc cgggtccgtc caggcgaaca
    12421 tcatcctggg caataccacc tatggcccgc tcgattcgtc gtattacgcc acccgcgtgg
    12481 ccatgcaggt cctcggtggt ggcgccgacg cgcggctctt cctgatcctc cgggaggcga
    12541 agggctggac gtacggagcg tacgcgggca tgaaccggta ccgcggcctg ggcttctggc
    12601 aggcgacgtt cgagggacgg accgcggtcg ccgacagcgc cctggccgaa atgcttcatc
    12661 aggtcgaccg gttgcgcacc gaaccgattc cagagaatga gctggccgcc gccaagggct
    12721 tcctggtggg ctcgttcccc ctgacgatcg aaacgtccga ccaggtagcg ggcgccgtgg
    12781 gcaacgccag gctgctgggc ctcggcaacc actacgtcca gacctatcgg gaacatctga
    12841 acgccatcac gtcggcgcag gccgaggccg cggcggcgaa ggctctcaag cgggaccacc
    12901 tcaccatcgt cgtggtcggt gacgcacagg cgct
//
LOCUS       DASUBP010000286         6265 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1264446, whole genome shotgun sequence.
ACCESSION   DASUBP010000286 DASUBP010000000
VERSION     DASUBP010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6265)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6265)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6265
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264446"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="VFU75_10940"
     CDS             complement(<1..857)
                     /locus_tag="VFU75_10940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467396.1"
                     /translation="MAVLALLAGGQSLAGQDNQRVWTNTLYPLVYYTSVDGFWAAGHF
                     AEYSSLGFKPGPQANAAAVSVDGAISTKGSYQLIVDAEAPALVGGWRAGLVLSATRDN
                     RLGFFGLGNATVNDPDSLSAANPYFYRVSRSRQAARLTIQRHIIGPFRALVGGQIEHT
                     SFRALPGASAFTRDLGAGIIDSTKVPFTDEMVRAGLVFDTRDVETVPHSGVVVEALYA
                     AGSGYNRRTASARLYVNPIEKLVLAGRLAAEEMGGHPPLAAQLNMESSDQTYVAVGGY
                     HSLRGYYDSR"
     gene            complement(869..1930)
                     /locus_tag="VFU75_10945"
     CDS             complement(869..1930)
                     /locus_tag="VFU75_10945"
                     /inference="COORDINATES: protein motif:HMM:NF013292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(4)-(beta-N-acetylglucosaminyl)-L-asparaginase"
                     /protein_id="HEU5467397.1"
                     /translation="MPDAMSRREFVAQTAQAGAVAATATAIPHAIRPPRALPATTRWA
                     GKPVIVSSANGIRGVQKAFDLIMGGADPLDAIIEGVKIQELDPTDHSVGYGGLPNAEG
                     VVQIDASCMHGPTRRAGAVGALEGIKTASEVVKCIMKYTDHIMLVGAGAQKFAVDYGF
                     KVEDLLTDEARDIWLHWRANLNPKDDYLDVPDQTTLVHRPTGTINMDAVTPGGEIASV
                     TTTAGLAFKIPGRVGDSAIVGAGQYTDNDVGAAGSTGLGEMNIMGTGAATIVDFMRRG
                     MKPTDACLEALKRVITMTDASHLTPDRKPKYQLNFYAVNKKGEFGCASFYAAQFAVHD
                     GTAAKLVDGGHLFDGQQPS"
     gene            2031..2504
                     /locus_tag="VFU75_10950"
     CDS             2031..2504
                     /locus_tag="VFU75_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467398.1"
                     /translation="MNLDGGDPRTAVRGVFFAVVVMATIGFVIAWVAEGHIDDKMAAL
                     LGVLWMFWIIASDVRRTLIDPATRWVRGQVVGGVADGVPMITADEEIAYLERLAADPT
                     ADRHRQIIGGIRLAELYRTVRKDQAKSDALLGQLAARYPDAPELEIARRFTPHDS"
     gene            2494..3213
                     /locus_tag="VFU75_10955"
     CDS             2494..3213
                     /locus_tag="VFU75_10955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEU5467399.1"
                     /translation="MIPELSLVVPVYNERENLAPLLTEIAAALRPTGRRYEVVAVDDG
                     STDGSRDELRRLRAEHPELHLVGFERNAGQTAAFAAGFRAARAPVVVTLDADLQNDPA
                     DIPGLVQELERTGATAVVGYRLNRRDTGWKRVQSRIANGVRNWLTSESIRDTGCSLKA
                     FRTDALRNLPFFNGMHRFLPTLVKMEGGTVAEVPVHHRQRRFGTTKYGMWNRVFRALA
                     DAFAVRWMQRRVMRYRINEES"
     gene            3213..4271
                     /locus_tag="VFU75_10960"
     CDS             3213..4271
                     /locus_tag="VFU75_10960"
                     /inference="COORDINATES: protein motif:HMM:NF014769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467400.1"
                     /translation="MARMARLDPSIFHLPVERMREGYYSDKYFVRARELLRKDGHRPR
                     VTMQVFGKTQGFLGGIDEAIAILKLCADRWDELTVSALYDGDQIAPWETVLLIEGPYD
                     VFAHLETLYLGVLARRTRVGTNTHAVVEAARPKQVMFFPARHDHWLVQTGDGYAAHIA
                     GAIGVSTDAQASWWGSEGIGTVPHALIAAYGGDTVLASKKFADLSDPNVALITLVDFD
                     NDCVGTSLKVAKALGSRLYGVRLDTSETLVDRSIVPQMGAFKPTGVNPQLVRNVREAL
                     DREGFQQVKIVVSGGFNVEKIRAFEEQGVPVDMYGVGSSLFQGRYDFTADVVRVDGRD
                     CAKVGRRFLPNPRLERVE"
     gene            4274..5173
                     /locus_tag="VFU75_10965"
     CDS             4274..5173
                     /locus_tag="VFU75_10965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU5467401.1"
                     /translation="MLRIDLTGKRAFVAGVSDDGGFGFAISKALAEAGATICLGSWPP
                     ALGIFTKLMERGKMDASMNLSDGRKLEFEKIYPLDAAYDTLGDAPAEVRENKRYKEVG
                     DFSIEGVKGLIEKDFGATPLDIVVHSLANAPEVKKPLLDTSRAGYLSAVSVSAYSMVS
                     LVRALGPLMRPGGSFLSLTYMAGERVIPGYGGGMSSAKAALEADTRTLAYEAGRRHGV
                     RVNTISAGPWASRAASAIGFIDMMINYTKANSPLPRSIDASDVGNTAAFLASPLAAAI
                     TGTTVYVDNGYHAMGMAVTPSAP"
     gene            5228..6187
                     /gene="argC"
                     /locus_tag="VFU75_10970"
     CDS             5228..6187
                     /gene="argC"
                     /locus_tag="VFU75_10970"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HEU5467402.1"
                     /translation="MNNYASVPVGVLGASGYAGRELLSLLNRHPGASVAFATAETGAG
                     ETLEGHRLIRAEDANFSSAEIVLSCLPHGVSGRYVHEARAAGKRAVDLSADFRLSPEA
                     VYGLTEFTRPKVAEADLVANPGCYPTAALMALVPLAQRGLIDPSREIVIDAASGVTGA
                     GRSPKRELLFGEVSEDYRAYAVGNAHRHLAELKSSLAREGGFNGDLVFTPHLLPVKRG
                     ILETIHVPLTRVIDQATAEGIYEAAYDGEPCVMVTKGSTPSLQHVVYRNRVAIGVAPV
                     TNVRRPRLTIIAAIDNLVKGAAGQAVQNMNVMLGLQETAGLAC"
BASE COUNT          992 a         2052 c         2163 g         1058 t
ORIGIN      
        1 cgcgagtcgt agtagccgcg gagcgagtga tagccgccca cggcgacata ggtctggtcg
       61 ctcgactcca tgttgagctg agccgccaga ggcgggtggc cgcccatttc ttccgcggcc
      121 aggcggcccg ccagcacgag cttttcgatg ggattgacat agagacgcgc cgaagccgtg
      181 cggcggttgt agccactccc cgccgcgtag agcgcttcga ccaccacgcc cgagtgcggg
      241 acggtctcta cgtcgcgcgt atcgaatacc agcccggcgc gaaccatctc atccgtgaaa
      301 gggaccttgg tggagtcgat gatgccggcg ccgaggtccc gggtgaaagc gctcgcgccg
      361 gggagcgcgc ggaaactcgt gtgttcgatc tgaccgccga ccagggcccg gaaagggccg
      421 atgatatgcc gctggatcgt gagccgcgct gcctgacgag agcggctcac ccggtagaag
      481 taggggttcg cggctgagag gctgtcgggg tcgttgacgg tcgcgttgcc cagcccgaag
      541 aagccgaggc ggttgtcgcg ggtggcggag aggacgaggc cggcccgcca tcccccgacc
      601 agcgccggcg cctccgcatc cacgatgagc tggtaactgc ccttcgtgct gatcgcgccg
      661 tcgacgctga cggccgccgc attcgcctgc ggccccggct tgaaccccaa tgacgaatac
      721 tcggcgaagt gaccggcggc ccagaacccg tccacgctcg tgtagtagac gagcgggtac
      781 agagtgttgg tccacacccg ttggttgtcc tggccggcga gcgactgccc gccggccagc
      841 aacgcgagta cggccatcca gggccgcttc aggagggttg ctgtccgtcg aacaggtgac
      901 cgccgtccac gagcttcgcc gccgttccgt cgtgcacggc gaattgcgcg gcataaaacg
      961 acgcgcatcc gaactcaccc ttcttgttga ccgcgtagaa gttgagctgg tatttcggtt
     1021 tccggtccgg cgtcagatgc gacgcatcgg tcatcgtgat cacgcgcttc agggcctcga
     1081 ggcaggcgtc ggtcggcttc atgccccggc gcatgaagtc cacgatggtc gccgcgccgg
     1141 tgcccatgat gttcatctca ccgaggccgg tcgatcccgc ggcgccgacg tcgttgtccg
     1201 tgtattgacc cgcgccgacg atcgccgagt cgcccacgcg gccgggaatc ttgaacgcca
     1261 ggccggcggt ggtggtcacc gacgcgatct ctcctccggg cgtcaccgca tccatgttga
     1321 tggttccggt cggccggtgc accagcgttg tctggtccgg aacgtcgaga tagtcgtctt
     1381 tggggttgag gttggcgcgc cagtgcagcc agatgtcgcg ggcttcgtcg gtgaggaggt
     1441 cttcgacctt gaagccgtag tccaccgcga acttctgtgc accggcaccc acgagcatga
     1501 tgtggtcggt gtacttcatg atgcacttca cgacttccga cgccgtcttg atgccttcga
     1561 gggcgcccac ggcgcctgcc cggcgggtcg ggccatgcat gcacgacgca tcgatctgga
     1621 cgacgccttc ggcgttcgga agtccgccgt agcccaccga gtggtcggtg ggatcgagct
     1681 cctggatctt cacaccctcg atgatggcgt cgagcggatc ggcgcccccc atgatcaggt
     1741 cgaacgcctt ctgcacgccg cggatgccgt tggcgctcga gacgatcacc ggcttcccgg
     1801 cccagcgggt ggtcgcgggc agagcccggg gaggcctgat ggcgtgggga atggcggtgg
     1861 cggtggcggc gacggcgccc gcttgggcgg tctgggcgac gaactcccgg cgggacatgg
     1921 cgtcaggcac ggctcgctcc tcgaagaagt gcgttggcgc cgtaggttta gggccgagag
     1981 cggcccgtgt caacttgctg gcccccccac ggtcctctac gttccggtcc gtgaacctgg
     2041 acgggggcga tcccaggacc gccgtgcgcg gtgtgttctt cgccgtggtc gtcatggcga
     2101 ccatcggatt cgtcatcgcc tgggtcgcgg aaggccacat cgacgacaag atggccgcct
     2161 tgctgggcgt gctctggatg ttctggatca tcgcctccga tgtccgccgg acgctgatcg
     2221 atccggccac ccggtgggtc cggggccagg tggtgggcgg cgtcgccgac ggcgttccga
     2281 tgatcacggc agatgaagaa atcgcctacc tggaaaggct ggcggctgac cccaccgccg
     2341 accggcaccg gcagatcatc ggcggcatcc ggctggcgga gttgtatcgc acggtacgaa
     2401 aggaccaggc caagtccgac gcactgctgg gccagttggc ggctcgctat cccgatgcac
     2461 cggagctcga gatcgcccgg aggttcacgc cgcatgattc ctgagctctc gctcgtcgtt
     2521 ccggtttaca acgagcgcga gaacctggcg cccctgctca cggagatcgc ggcggccctc
     2581 cgtcccaccg gccggcgcta tgaagtcgtc gcggtcgacg acgggtcgac ggacggctcc
     2641 cgcgacgaac tgaggagatt gcgcgcggag cacccggagc tgcatctggt ggggttcgag
     2701 cgcaacgccg gacaaaccgc ggccttcgcc gccggatttc gggctgcgcg ggcgccggtc
     2761 gtggtgaccc tggatgccga tcttcagaac gatccggccg acatccccgg gctcgtccag
     2821 gagctggaac gcaccggcgc gacggccgtg gtggggtacc ggctcaatcg ccgggatacg
     2881 ggatggaagc gcgtgcagag ccgcatcgcg aacggggtgc gcaactggct gacctccgag
     2941 agcattcgtg acaccgggtg ctcgctgaag gcgttccgga ccgacgccct ccggaatctc
     3001 ccgttcttca acgggatgca ccgctttctg ccgaccctgg tgaagatgga aggcggtacg
     3061 gtggcggagg tacccgtgca tcaccggcag cgccgcttcg ggaccaccaa gtacgggatg
     3121 tggaatcgcg tcttccgcgc tctggctgac gcgttcgcgg tgcggtggat gcagcgacgg
     3181 gtgatgcgct accggatcaa cgaggagagc tgatggcgcg gatggcacgc ctcgacccct
     3241 ccatcttcca tctgcccgtc gagcgcatgc gcgagggcta ctactcggac aagtatttcg
     3301 tccgcgcgcg cgagctgctt cgcaaggacg gccatcgccc gcgagtcacg atgcaggtgt
     3361 tcgggaagac gcaggggttc ctgggcggca tcgacgaagc cattgccatc ctcaagcttt
     3421 gtgccgaccg ctgggacgag ctgaccgtct cggcgctcta cgatggcgat cagatcgcgc
     3481 cctgggagac ggtgctcctc atcgaaggcc cgtatgacgt cttcgctcac ctggagaccc
     3541 tgtatctggg cgtactggcc cgccggaccc gggtaggcac caacactcac gctgtggtcg
     3601 aggcagcccg cccgaagcag gtgatgttct ttccggcgcg gcacgaccac tggctggtgc
     3661 agaccgggga cggctacgcg gcccatattg ccggggccat cggggtctcg acggacgcac
     3721 aggcgtcctg gtggggctcc gaagggatcg gcacggtgcc gcatgcgttg atcgccgcgt
     3781 atgggggtga caccgtcctc gcgtcgaaga agtttgcgga cctgtcggat cccaacgtcg
     3841 cgctgatcac gctggtcgac ttcgacaacg actgcgtcgg gacctcgctg aaggtcgcga
     3901 aggcgctggg gtcccggctg tacggggtcc gcctcgatac ctcggaaacg ctggtcgaca
     3961 ggtcgatcgt gccccagatg ggggcgttca agccgaccgg cgtgaatccg cagctcgtcc
     4021 gaaacgtgcg cgaggcgctg gaccgcgagg ggttccagca ggtgaagatc gtggtgtcgg
     4081 gcggattcaa cgtcgagaag atccgggcgt tcgaagagca gggcgtgccg gtggacatgt
     4141 acggcgtggg gtcgagcctc tttcagggac gctacgattt caccgccgat gtggtgcgcg
     4201 tcgacggccg ggattgcgcc aaggtgggcc ggcgcttcct gcccaatccc agactcgaac
     4261 gggtggagtg accatgttgc ggatcgacct gacggggaag cgggccttcg tcgcgggcgt
     4321 gtcggatgat gggggcttcg gcttcgccat ctcgaaggcg ctggccgagg ctggtgccac
     4381 catctgcctc gggagctggc ccccggcgct cggcatcttc accaagctca tggagcgggg
     4441 gaagatggac gcatccatga acctctcgga cgggcggaag ctcgagttcg agaagatcta
     4501 cccgctcgac gccgcctacg acacgctcgg cgacgccccc gcggaggtcc gcgagaacaa
     4561 gcggtacaag gaggtcgggg atttcagcat cgagggagtg aagggcctca tcgagaagga
     4621 tttcggggcc acgccgctcg acatcgtcgt gcactccctg gccaacgcgc ccgaggtcaa
     4681 gaaacccctc ctcgacacca gccgggccgg gtacctctca gcggtctccg tttcggccta
     4741 cagcatggtg tccctggtcc gggccctggg cccgttgatg cgccccggcg gctccttcct
     4801 ttcactgacc tacatggccg gggagcgtgt catcccgggc tatggcggcg gcatgtcctc
     4861 cgccaaagcg gccctcgagg ccgacacccg gaccctcgcc tacgaggcgg gaaggcggca
     4921 tggcgtccgc gtgaacacca tctcggcggg accctgggct tcccgggccg cctcggccat
     4981 cgggttcatc gacatgatga tcaactacac caaggccaat tcgcccctcc cgcggtcgat
     5041 cgacgcatcg gacgtgggga acaccgcggc cttcctggcc agccccctcg ccgccgcgat
     5101 caccgggacc acggtgtatg tcgacaacgg gtaccacgcg atgggcatgg cggtgactcc
     5161 ctcggcgccc tgaaaaagcc ggaaaagccc ttgcgtccgg cgggatgatg cataaattcc
     5221 cgctgagatg aataactatg catcggtccc ggtcggggtt ctcggggcct ccggatacgc
     5281 gggacgggag ctgctgtcgc tcctgaaccg gcacccgggg gcctccgtcg ctttcgccac
     5341 cgccgagacg ggcgcgggcg agaccctcga aggccaccgg ctcatccggg ccgaggatgc
     5401 caacttctcc tccgccgaaa tcgtgctgtc ctgcctgccg cacggcgtgt cgggccggta
     5461 cgtccacgag gcgcgggccg ccggcaagcg cgccgtcgac ctctccgcgg atttccggct
     5521 ctcacccgaa gccgtctacg ggctcaccga gtttacgcgc ccgaaagtcg ccgaggccga
     5581 tctcgtcgcg aatcccggtt gctatccgac cgcggcgctg atggcgctcg tgccgctggc
     5641 gcagcgcggc ttgatcgacc cctcgcggga aatcgtgatc gacgcggcgt ccggcgtgac
     5701 cggcgcgggc cggagtccca agcgggagct cctgttcggc gaggtctccg aggactaccg
     5761 ggcctatgcg gtggggaacg cccatcggca cctggccgag ctgaaatcgt cgctcgcgcg
     5821 ggaaggcgga ttcaacggcg atctggtctt cacgccgcac ctgctccccg tgaagcgcgg
     5881 gatcctcgag acgattcacg tgccgctcac gcgggtcatc gatcaggcga ccgccgaggg
     5941 tatctatgaa gccgcgtacg acggcgagcc ctgcgtcatg gtgaccaagg ggtcgacccc
     6001 gtcgctccag cacgtcgtct accggaatcg cgtggcgatc ggcgttgcac cggtgacgaa
     6061 cgtccggcgt ccccggctca cgatcatcgc ggccatcgac aacctggtca aaggcgcggc
     6121 ggggcaggcg gtgcagaaca tgaacgtgat gctgggcctc caggaaacgg cggggctcgc
     6181 gtgctgaagg ttcgcgtcgt caagatcggc ggcaacgagc tcgatcgccc cgagtgggtg
     6241 tcctcgtgcg cccgggccct gtccg
//
LOCUS       DASUBP010000287         2619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_269751, whole genome shotgun sequence.
ACCESSION   DASUBP010000287 DASUBP010000000
VERSION     DASUBP010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_269751"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..1021
                     /locus_tag="VFU75_10975"
     CDS             32..1021
                     /locus_tag="VFU75_10975"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5467403.1"
                     /translation="MTNAPTDLVGQTLGPYAIEQLLGQGGFAWVFAAREKDGTPVAVK
                     VLKPRYANDPQFAPRFRNEAKTASQLEHPNIIRIRAVGESDGHSYFSMDLCADGLGLR
                     IAREGPLPEADLVRIGTDIAGALEFAHAQGIIHRDIKADNVLLRSDGSAVLTDFGIAR
                     TVSGYATSTGVDMTIGTPNYISPEQAQGRPVDPRADIYSLGVTLYKAATGEVPFTSKD
                     WFELARMHVEDPPPPPRARRPDLSKRLERVIFKCLAKHPDDRYATATELLADLVDVEA
                     KHRPTLEIGTPPPGTREQVAIKRPVKKRFPWEIVVLGVAVVVLAVLVARLIRR"
     gene            complement(1059..1442)
                     /locus_tag="VFU75_10980"
     CDS             complement(1059..1442)
                     /locus_tag="VFU75_10980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467404.1"
                     /translation="MSSTRETLAEYVGGRIKADRLVAAVAAAYYRERKALKGEGWREL
                     IAVIERGAPGVVELAAAEGKGFEIKLAERPFPSAFEPALRAAAQRALSEVPASISADQ
                     TPTVTLPSRGFFGRMIDRVAKWLSR"
     gene            complement(1429..>2619)
                     /gene="rseP"
                     /locus_tag="VFU75_10985"
     CDS             complement(1429..>2619)
                     /gene="rseP"
                     /locus_tag="VFU75_10985"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEU5467405.1"
                     /translation="IAYLPFGGYVKMAGLEEEGMMGEVEGGAATVPVDPERAFDRKPL
                     VVRSAVMLAGVTMNFLFAWAVYSGIAASVGTERIATTQIDTVMAGSLPPGAEALGTIK
                     RGERIVAVNGDSVHSWEGVGEAIGFAHLPISITVAGRPEPIVLSSIRDGQDRATAFGA
                     LVPAYPAKLKEVAPGTPAERAGLRAGDVVIRAGADTIANWGQFQRAIVSNPHRRVELG
                     VRRGDSTLTLAITFDAHPDTAKGKGPADSIGYAGVRWDPPIVRTKYSVTGAVGEGWRQ
                     SEHALGIVTGSLRMLVTREASLRDLGGPIAIGKISGDQAREGWPSLLQWMAILSVNLA
                     LLNLLPIPILDGGQMVFLIAEGIRRRPLSIELRLRLTQVGFIFIVALMLFVVGNDLIR
                     YVIH"
BASE COUNT          463 a          926 c          811 g          419 t
ORIGIN      
        1 tagaggctca tcgcgcctct gtaaaggcct catgacgaac gccccgaccg acctcgtcgg
       61 ccagacgttg ggcccatacg ccatcgaaca actgctggga cagggcggtt tcgcctgggt
      121 ctttgccgcc cgggagaagg acggcacccc cgtcgcggta aaagtattga agccgcgcta
      181 cgcgaatgac ccccagttcg ccccccggtt ccggaatgag gccaagaccg cctcccaact
      241 cgagcatccg aacatcatcc ggatccgcgc cgtgggggaa tccgacgggc attcgtactt
      301 ctccatggat ctctgtgccg acggcctggg gctccggatc gcccgggagg gccccctccc
      361 cgaggcagac ctggtccgga tcgggacgga catcgccggg gccctcgaat tcgcgcacgc
      421 ccagggcatc atccaccggg acatcaaggc cgacaacgtg ctgctccgat ccgacgggtc
      481 cgccgtgctg acggacttcg ggatcgcccg gacggtgtcg ggttatgcga cctcgacggg
      541 ggtcgacatg acgatcggga cgccgaacta catctcgccg gagcaggcgc agggccgccc
      601 cgtggatccc cgggcggaca tctactccct cggcgtcacg ctctacaagg ccgcgaccgg
      661 cgaggtgccc ttcacctcga aggactggtt cgagctggcc cgcatgcacg tcgaggatcc
      721 gccacctccc ccccgcgccc gccgtcccga cctctccaag cggctcgagc gggtgatctt
      781 caagtgtctc gcgaagcatc cggacgaccg ctacgccacc gccaccgagc tgctcgcgga
      841 cctcgtggac gtcgaggcga agcaccggcc gacgctggaa atcgggacgc cgcctcccgg
      901 aacgcgggag caggtcgcga tcaagcgtcc ggtgaaaaag agattcccgt gggaaattgt
      961 ggtgctaggg gtggccgtcg tggtgctggc agttttggtc gcgaggctga taagacgcta
     1021 agacgctaag acgccaagtc gctaggtcac ttagcgacct agcgacttag ccacttggca
     1081 actctatcga tcatccgccc gaagaatccg cgacttggta gggtcaccgt gggagtttgg
     1141 tctgccgaga tgctggcagg cacttcagag agcgcccttt gcgccgcagc tcgtagtgcg
     1201 ggttcgaagg ccgagggaaa gggccgctcg gcgagcttga tctcgaagcc cttcccctcg
     1261 gccgcggcga gctcgaccac acccggcgcg ccgcgctcga tcaccgcgat cagctcgcgc
     1321 cacccctcgc ccttcaaggc tttgcgctcc cggtaatacg ccgcggcgac cgccgcgacc
     1381 agccggtcag ccttgatccg cccgccgacg tactcggcga gcgtctctct agtggatgac
     1441 atagcgaatc agatcgttcc cgaccacgaa gagcatcaac gccacgatga agatgaaccc
     1501 cacctgcgtg agccgcagcc gcaattcgat ggacagcgga cgtctccgga ttccctcggc
     1561 gatcaggaac accatctggc caccgtcgag aatcggaatc ggcaggaggt tcaacagggc
     1621 aaggttcacc gaaaggatgg ccatccactg caagaggctt ggccatccct cgcgggcctg
     1681 atccccggag atcttcccga tcgcaatcgg gcctcccagg tcgcggagcg acgcctcgcg
     1741 ggtcacgagc atgcgcagac tgcccgttac gatccccagc gcatgctcgg attgccgcca
     1801 tccctccccg acggcacccg tcacactgta cttcgtccgc acgatcggcg gatcccaccg
     1861 tacaccggca tacccgatcg agtcggccgg ccccttcccc ttcgcggtat cggggtgtgc
     1921 gtcgaacgtg atcgcgagcg tcagcgtcga atcaccccgc cggacaccaa gttccacgcg
     1981 ccggtgcgga ttgctgacga tggcacgctg gaattgcccc caattcgcga tcgtgtcggc
     2041 gccggcgcgg atcaccacgt cccccgcgcg caaccccgcg cgctcggcgg gcgtgccggg
     2101 agcgacttct ttcaatttgg cgggataggc cggcaccagc gcgccgaacg cggtggcgcg
     2161 gtcctgaccg tctcgaatgg acgaaagaac gatgggctcc ggtcgtcctg cgacggtgat
     2221 gctgatcggc aggtgcgcaa acccgatggc ttcgcccacg ccctcccacg agtggacgga
     2281 atcgccgttc acggccacga tccgctcacc acgtttgatg gtgcccagag cctcggcgcc
     2341 gggaggcagg ctgccggcca tgaccgtgtc gatctgggtg gtcgcgatcc gctccgtccc
     2401 gacgctcgcg gcaatgccgg agtagacggc ccaggcgaac aggaaattca tcgtgacccc
     2461 ggccagcatc accgcgctgc ggaccaccaa tggcttccgg tcgaaggcac gctccgggtc
     2521 gacgggaacc gtcgccgcgc cgccctcgac ctcccccatc atcccttctt cttccagtcc
     2581 ggccattttc acgtagccgc cgaacgggag gtacgcgat
//
LOCUS       DASUBP010000288         5884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_146765, whole genome shotgun sequence.
ACCESSION   DASUBP010000288 DASUBP010000000
VERSION     DASUBP010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 5884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5884
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146765"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..778
                     /locus_tag="VFU75_10990"
     CDS             <1..778
                     /locus_tag="VFU75_10990"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5467406.1"
                     /translation="ENEDVTGMAPAARRFGMVFQHYALFPHLNVGENVAFGLARGSDR
                     AAQVAEALGLVDLAGFEKRKVQELSGGQQQRVAVARALAPHPRVLLLDEPLSNLDPSL
                     RERTRRELREVIGRVGITTVFVTHEQEEAFDLGDRVAVLNGGRLEQIGSADQVYDTPS
                     SLFVATFVGRANVLKGSAALAFGGEPGQALVIRPEHLRIVKGPGVAGVVKERRFTGAS
                     AFLLVEIASGARVEVLSEPRAAVVGDHVTLAAVRKAVFPA"
     gene            775..2394
                     /locus_tag="VFU75_10995"
     CDS             775..2394
                     /locus_tag="VFU75_10995"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEU5467407.1"
                     /translation="MRRRSSSGLLLTLLLGWLVLYPVLIVVVDAGHATAIHAFVSRST
                     EWRALWASCWISVASVALAAVIGIPLAFLFEWFEFPGRKLLGSLIALPAVLPPLVGVI
                     AFLFLYGESGFVARALQYVFRFQQAPWQLQGPGAILLVHAYSMYVYFYLFTRAGLAKV
                     DAAMIEAAHALGAGRWRTLFRVTLPLLRPSLAGATLLTFMTALGSFSAPYVFGGGFRV
                     MPTQIVASRLNGEDALAMVETVALALLALIGLVLLRRTEGDVGVVSLGKGAAPQRRVI
                     RTPMARVTATVAGWVLAVVLLLPHLTLILVSLVPYATWTTEAFPPVLNLDNYRRLLEE
                     PARLAPLVNSLWMAVVSTAGAVVLALAAGWMVVRRRARLSRIIEALVALPWALPGTVF
                     AIALATTFSVMKPLEARWVLIGTAAILPLAYLVRNIPVTGRAILAGYRQLDPAFDEAA
                     ASLGAGRWQTFRRVSLPLLSPALMAGASLAFVAALGDFVTSVVLYTYDTRPISMEILS
                     SLRLNEIGVAAAFGVVLMIVSAVVLAIGARR"
     gene            2409..3614
                     /locus_tag="VFU75_11000"
     CDS             2409..3614
                     /locus_tag="VFU75_11000"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEU5467408.1"
                     /translation="MKRLIVLIAVCFVDMVGLMIIAPLMPFYALRFHAQDWMVGWLYS
                     AFSVAQLVASPLWGRFSDRYGRRPALIAGLLGACGAYLVFGLATSLTVLFLSRIVQGL
                     GGGTTGVAQAYVADAMEPADRAKALGWVSAGTSLGVIVGPPLGGLATTIGPAAPGLIA
                     AALVFINAICAWKWLPESRHQYSTPNPAVPAKSPRSVQATLWEIVRHPARPAPRVIWI
                     YVIGMLALTGLIGVLALYLKDTFGVTEKGIGYVFMVFGAVGVVMRTAPVGWINAWLGE
                     VRTMRLGAALVLLGLALIPLPPVLFLCVACLVLVPIGNALLFPASTSLVSQRTERAEM
                     GQVMGAQQTLRGIAAIIGPAGATAAYQLFGHRIPFTAAAVVVAVALFLTTREPHAQPV
                     ADAPATIKA"
     gene            3633..4025
                     /locus_tag="VFU75_11005"
     CDS             3633..4025
                     /locus_tag="VFU75_11005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467409.1"
                     /translation="MAGVKLSASQQRRVDQITELQKIVGHTRATLKELESNRAAKATV
                     VNNLSGMIAREMQQMRQRLLTSPVGTVGDLAGNLSVVASRSQGMNVKIRALNDGLNSI
                     DMQLDLAMKNAMQPEKDDKHAAGEQPAH"
     gene            4006..4980
                     /locus_tag="VFU75_11010"
     CDS             4006..4980
                     /locus_tag="VFU75_11010"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5467410.1"
                     /translation="MSSPRTDGPGGPDGPAIRTAGLVKKYDDVTAVAGLDLTIHRGEC
                     FGLLGPNGAGKTTTIEILEGLTLPDAGEVEVLGMQWSDEAGGRALREHLGIQLQETQL
                     AEKLSVNETLSLFRSFYETSNTVDEVLDLVSLGEKKESWVGKLSGGQKQRLAVACALV
                     GKPDLLFLDEPTTGLDPQSRRQLWEVVERFKGQGHTVLLTTHYMDEAERLCDRVAIMD
                     HGKVIALGTPRELIASLGAEHVIEFALANGGSPADLKPELEKLPGIRSVRLTADGLSL
                     TAAELHTVLPALMAALQRRHIALASLTTHHATLEDVFVSLTGRQLRDA"
     gene            4973..>5884
                     /locus_tag="VFU75_11015"
     CDS             4973..>5884
                     /locus_tag="VFU75_11015"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5467411.1"
                     /translation="MRNHPIFQLTISRVREFYREPEAIFWVFGFPIVLAAALGLAFRN
                     KGPSAISVGVRSGAGDSAVVAALKADSALRPIVLDSATAGARLRGGSIAILVVPGNPV
                     TYRFDPTRDESRPARLEVDQALQRAAGRADPVATTNDHVTARGSRYIDFLIPGLLGMN
                     LLGSGIWGVGFAVVNARQKKLLKRFMATPMRKSHYLLGFILSRLVFLVVEVAALVGFG
                     WVIFGVGVRGSWLALAGIALLGSLCFSGLGLLIASRARTIEGVSGLMNLAMLPMWILS
                     GTFFSYSRFPDVMLPFVKALPLTALNDS"
BASE COUNT          828 a         1928 c         2037 g         1091 t
ORIGIN      
        1 ggagaatgaa gacgtgacgg ggatggcgcc ggcggcgcga cggttcggca tggtgtttca
       61 gcactacgcg ctcttccctc atctgaatgt cggggagaac gtcgcgttcg gtctggccag
      121 gggcagtgac cgcgcggcgc aggtcgctga agccctgggc ctcgtggatc tggccgggtt
      181 tgaaaagcgg aaggtgcagg agctctcggg gggtcaacag cagcgcgtcg cggtggcccg
      241 ggcgctcgcg ccccacccgc gcgtgctctt gctggacgag ccgctctcca atcttgatcc
      301 ctcccttcgc gaacgcaccc ggcgcgagct ccgcgaagtg atcggccggg tgggcatcac
      361 taccgtcttc gtcacgcacg agcaggaaga ggccttcgat ctcggcgacc gtgtcgccgt
      421 gctgaatggt ggccgcctgg agcaaatcgg gagcgccgat caggtctacg acactccctc
      481 gagcctcttc gtcgcgacgt tcgtcggccg cgccaacgtg ctgaagggca gcgcggccct
      541 ggcgttcggt ggggagccgg gacaggcgct cgtgattcgc ccggagcatc tccgcatcgt
      601 gaagggcccg ggtgtcgccg gggtggtcaa ggaacgccgc tttacgggcg cgagcgcctt
      661 tctcctggtc gaaatcgcga gcggcgcgcg cgtcgaagtg ctgtcagagc cccgtgccgc
      721 cgtggtgggg gaccacgtca ctctcgcggc cgtgcgaaag gccgtgttcc cggcatgagg
      781 aggcgctcgt cgtccggcct gctgctcacg ctgctgctcg gctggctggt gctctatccg
      841 gtgctcatcg tggtggtcga cgcggggcac gcgaccgcga tccacgcctt cgtgtcccgg
      901 agcaccgagt ggagggcact ctgggccagc tgctggatct cggtggccag cgtcgccctg
      961 gcggcggtga tcggcatccc gctcgccttc ctcttcgagt ggttcgagtt cccgggacgc
     1021 aaactcctcg gctcgttgat cgcgctcccc gccgtgctgc ctccgctggt gggcgtcatc
     1081 gccttcctct ttctctacgg cgaatcaggt ttcgtcgcgc gggccctgca gtacgtcttc
     1141 cgctttcaac aggcgccctg gcagctgcag ggaccggggg cgatcctgct cgtccatgcg
     1201 tattcgatgt acgtctactt ctatctcttc acccgggcgg gactcgcgaa ggtcgacgcc
     1261 gcgatgatcg aggcggcgca cgcactcggc gcggggcgct ggcggacact ctttcgggtg
     1321 accctgccgc tcctccggcc ctcgctggcc ggcgccacac tgctcacgtt catgaccgca
     1381 ctcgggtcat tctccgcgcc gtacgttttc ggcggcggtt tccgggtcat gccgacccag
     1441 atcgtcgcct cccggctgaa tggcgaagac gcgctggcga tggtcgagac ggtggccctg
     1501 gcgctcctgg cgctgatcgg gctcgtgctg ttgcggcgca ccgagggtga cgtcggagtg
     1561 gtgtccttgg ggaaaggcgc ggcgcctcag cgccgtgtga tccgaacgcc gatggcgcgg
     1621 gtgacggcga cggtggcggg gtgggtgctg gcagtggtgc tgctcctgcc ccatttgacc
     1681 ctgattttgg tctcgctcgt cccatacgca acgtggacaa ccgaagcctt ccctccggtg
     1741 ttgaacctgg acaactatcg ccgcctgctc gaggagccgg cgcggctcgc gccgctcgtg
     1801 aactctctct ggatggcggt ggtgagtacg gcaggggccg tcgtcctggc cctcgcggcc
     1861 gggtggatgg tggtgaggcg ccgggcgcgg ctgtcccgga tcatcgaggc gttggtggcg
     1921 ctgccgtggg cgttgcccgg gacggtcttc gcgatcgccc tggcgaccac gttcagcgtg
     1981 atgaaacccc tggaggctcg ttgggtgttg ataggaacgg cggcgatcct cccgttggcc
     2041 tatctggtgc ggaatattcc ggtgacgggg cgggcgattc tggcaggata ccggcagctc
     2101 gatccggcat tcgatgaagc ggcggcgagt ctcggggcgg ggcggtggca gacgttccgg
     2161 cgggtgagcc tgccgctcct gtcgccggcg ctgatggcgg gcgccagtct ggccttcgtg
     2221 gcggccttgg gtgacttcgt gacgtcggtg gtactctata cctacgacac gcgtcccatc
     2281 tcgatggaaa ttctgtcgtc gctgaggctg aacgaaatcg gcgtggcggc cgcgttcggt
     2341 gtggtgctca tgatagtcag tgccgtcgtc ctcgccattg gagcccgtcg gtgaacctga
     2401 gcccttctgt gaagcgcctg atcgtgctga tcgccgtgtg cttcgtcgac atggtcggtc
     2461 tgatgatcat cgccccactc atgccgttct acgccctccg attccacgcg caggactgga
     2521 tggtcgggtg gctgtacagc gcattctcgg tggctcagct cgtcgcgtcg cccctgtggg
     2581 gccggttttc ggaccgctac ggccgacgcc cggcgctgat cgcggggctg ctgggtgcct
     2641 gcggggcgta cttggtgttc ggattggcga cgtcgctgac ggtactgttc ctctcgcgca
     2701 tcgtgcaggg tctgggcggc ggtaccaccg gcgtcgcaca ggcgtacgtg gccgacgcga
     2761 tggaaccggc ggatcgtgcc aaagcgctgg gatgggtttc cgccgggacg tcgctcggag
     2821 tgatcgtcgg tccacccttg ggagggctcg cgacgaccat cggaccggct gcccccggcc
     2881 tgatcgccgc cgcgctggtg ttcatcaatg ccatttgtgc ctggaagtgg ctccccgagt
     2941 cgcggcatca gtattccacc cccaacccgg cggttcccgc gaagtcgccc cgctcggtgc
     3001 aggcgacgct ctgggagatc gtccggcatc ccgcccgtcc tgccccccgg gtcatctgga
     3061 tctacgtcat cgggatgctg gcgctcaccg ggttgatcgg tgtcctcgcg ctgtacctca
     3121 aggacacctt cggagtcacg gagaagggca ttgggtacgt cttcatggta ttcggcgcgg
     3181 tcggcgtggt catgcggacg gcgccggtgg ggtggatcaa cgcgtggctg ggagaggttc
     3241 ggaccatgcg tctgggcgcc gccctggtct tgctggggct ggcgttgatc ccgctccctc
     3301 cggtgctgtt tctctgcgtc gcgtgtctgg tactggtgcc catcgggaac gccctgctgt
     3361 tccccgccag cacgtctctc gtgagtcagc gcacggaacg ggcggaaatg ggccaggtga
     3421 tgggagcgca gcagaccctt cgcgggattg cggcgatcat cggcccggct ggggccaccg
     3481 ccgcgtacca gctgttcggg caccggatcc ccttcaccgc cgcggccgtg gtggtggcgg
     3541 tggcgctctt cctcaccacg cgggagcccc acgcacagcc ggtcgcggat gccccggcta
     3601 ctattaaggc ctgacccagg ggggagtacc ggatggcggg cgtcaaactc agtgcgagcc
     3661 agcagcggcg agtcgatcag atcaccgagc tccagaagat cgtcggtcac acccgcgcga
     3721 ccttgaaaga gctggaatcg aaccgcgcgg ccaaggccac ggtggtgaac aacctctcgg
     3781 ggatgatcgc ccgcgaaatg cagcaaatgc gccagaggct gctcacctcc cccgtgggca
     3841 ccgtgggcga cctcgccggc aacctgtccg tcgtggccag ccggagccag gggatgaacg
     3901 tgaagatccg ggcactgaac gacggcctga acagcatcga catgcagctc gacttggcga
     3961 tgaagaacgc catgcagccc gagaaagacg acaagcacgc ggccggtgag cagcccgcgc
     4021 actgacggac ccggcggccc ggacggccca gccatccgca ccgcgggact cgtcaagaaa
     4081 tacgacgacg tcacggcggt cgcgggactc gatctcacca tccatcgcgg cgaatgcttc
     4141 ggactcctgg ggcccaacgg cgccgggaaa accaccacga tcgagatcct cgaagggctg
     4201 accctccccg acgccggaga ggtcgaagtg ctggggatgc agtggtcgga cgaggcgggt
     4261 ggccgcgccc tccgcgaaca cctcggcatc cagctccagg agacccagct ggccgagaag
     4321 ctctcggtga acgagacgct gtccctcttc cggtcattct acgagacctc caacaccgtg
     4381 gacgaagtcc tcgacctcgt ctccctgggc gagaagaagg agagctgggt ggggaagctc
     4441 tccggggggc agaaacagcg tctggccgtg gcctgcgcgc tggtcggcaa gcccgatctg
     4501 ctcttcctcg acgagccgac gacgggcctc gacccgcaat cacgccgcca gctctgggaa
     4561 gtcgtcgagc ggttcaaggg gcaggggcat accgtcctcc tgaccaccca ctacatggac
     4621 gaggccgagc ggctctgtga ccgcgtggcg atcatggacc acgggaaagt gatcgccctg
     4681 ggaacgccgc gggagctcat cgcgtccctg ggcgcggagc acgtcatcga gttcgcgctg
     4741 gccaatggcg gctcgccggc ggatctcaaa cccgagcttg agaaattgcc ggggattcgg
     4801 agcgtccggc tgacggctga cggcttatcg ctgaccgctg ctgaattgca cacggtcctc
     4861 ccggcgttga tggctgcgct tcagcgccgg cacatcgccc tcgcatccct caccacgcac
     4921 cacgccaccc tcgaagacgt gttcgtgtcc ctcaccgggc ggcagctgcg cgatgcgtaa
     4981 ccatcccatc ttccagctca cgatctcccg cgtccgcgag ttctaccgcg agccggaggc
     5041 cattttctgg gttttcggat ttccgatcgt gctggccgcg gcgctcgggc tcgcgttccg
     5101 gaacaagggg ccgtcggcga tttcggtcgg cgtgcgcagt ggagcgggcg acagtgccgt
     5161 ggtcgccgcg ctgaaggcgg actccgcact ccgccccatc gtcctcgatt cggccaccgc
     5221 gggggcgcgg ctccgtggcg gcagcatcgc gatcctcgtc gtcccgggca atcccgtgac
     5281 gtaccggttc gacccgaccc gggacgagag ccgccccgcg cgcctcgaag tggatcaggc
     5341 gctgcagcgc gcggccggac gtgccgatcc ggtggccacg accaacgatc acgtgacggc
     5401 ccgcggctcc cggtacatcg actttctgat tcccggcctc ctcggcatga acctgttggg
     5461 ctcgggcatc tggggggtcg gcttcgcggt ggtgaacgcg cgccaaaaaa agctgctcaa
     5521 gcgtttcatg gcgacgccga tgcggaagag ccactatctg ctgggcttca tcctgtcgcg
     5581 cctggtcttc ctcgtggtcg aagtcgccgc gctggtgggg ttcggttggg tgatcttcgg
     5641 cgtcggggtg cggggttcgt ggctggcgct cgcgggcatc gccctgctgg gttcgctctg
     5701 tttctcggga ctcggactcc tgatcgccag ccgggcccgg accatcgaag gcgtgtcggg
     5761 cctgatgaat ctggcgatgt tgccgatgtg gattctctcg gggaccttct tctcgtattc
     5821 ccggtttccc gacgtcatgc tgcccttcgt gaaggcgctg cccctcaccg cgctgaacga
     5881 ctcg
//
LOCUS       DASUBP010000289         2875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_992226, whole genome shotgun sequence.
ACCESSION   DASUBP010000289 DASUBP010000000
VERSION     DASUBP010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_992226"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1485)
                     /locus_tag="VFU75_11020"
     CDS             complement(<1..1485)
                     /locus_tag="VFU75_11020"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HEU5467412.1"
                     /translation="MNRKQVGILILIIWAVSMAWLVRRVYFQSTGSRLANAALSVQPG
                     AAYYRVDVGGRQVGYASTTLDTLTDSLRVVDAEVIETAADGHLNRTTTRSEAKLSRAL
                     RLETVDVKYTEDGRAYTAAGIFTQDSTIKLAVSDGRLTARSETRTHRTPMIPSLMPLR
                     LAFGGEMKVGATFDTRVFDSPILITRDVHVRVAAETTLVNPDSAEFDSTTKTWVPVHF
                     DTTPAFRIEGSGTGGPFRLWIDPQGRVVREERPTGVVYNRTAFELAVQNFRHRDTLAI
                     KAASLSQRPGLVGTFPPERPMPPLTAMRVRLGGAPIAGFDLAGGGQALAGDTVTVRQV
                     ALDSLHPRYRVPLHDSLFTETLHSEPLMPVATMDVGFPLMRLIGQEREPVKVAGMITH
                     WVFQTARHDTTALRPEALLPLSSSKGDFDGVNTLFVTMSRSVGIPARRVAGVRRVGDR
                     FYYYEWAEVWLGQWVPVDAYNDQFPADASHLRLMIGMPGRRAVMT"
     gene            complement(1482..1946)
                     /locus_tag="VFU75_11025"
     CDS             complement(1482..1946)
                     /locus_tag="VFU75_11025"
                     /inference="COORDINATES: protein motif:HMM:NF013798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macro domain-containing protein"
                     /protein_id="HEU5467413.1"
                     /translation="MIRVVVADLASCPADAVVRPTSSHFDSLVTDSFGLGGAVVTAGQ
                     GAVAAEFVIQACIREEAHHAPTREMVSRAWRAALQQASEWEFRHVAAPILGADDGTGA
                     LPAKDVADLLVEVLEQHRARSAFPAEVSFVVANTDERDVIEAALRRASARAS"
     gene            complement(1943..2521)
                     /locus_tag="VFU75_11030"
     CDS             complement(1943..2521)
                     /locus_tag="VFU75_11030"
                     /inference="COORDINATES: protein motif:HMM:NF024823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEU5467414.1"
                     /translation="MTRRHLRRWPPVAERARVAAQLLAEAAAFTEDVGLLTGPLALAP
                     AGGMPKTRVPAPVTVVAPPPAPEPAPAPAAAPEPTAPTPVASTPVPAAMALAETGVTL
                     ARRGLWSQAVPQFRHAIELDPVNVPIWCRLGEALNHIDDLDGARLAYEKGLAVDEKHP
                     RALYGMGVVLDRLRRPEEATVYYRRAREATAR"
     gene            complement(2518..>2875)
                     /gene="cutA"
                     /locus_tag="VFU75_11035"
     CDS             complement(2518..>2875)
                     /gene="cutA"
                     /locus_tag="VFU75_11035"
                     /inference="COORDINATES: protein motif:HMM:NF015077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010038 - response to metal ion [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="divalent-cation tolerance protein CutA"
                     /protein_id="HEU5467415.1"
                     /translation="NSLANRTLAAPMATGALVVLTTLSTVEDARHLVHALVSERVIAC
                     GTVLPAGTSIYRWKNEIKEETEVVVLMKTEASRWDALVTAVQARHPYEVPELLALPVE
                     HGLEAYLSWIEHEVVT"
BASE COUNT          477 a          949 c         1005 g          444 t
ORIGIN      
        1 cgtcatgacg gcgcgacgcc cgggcatccc gatcatcaac cgcagatggg aagcatccgc
       61 ggggaactgg tcattgtagg cgtcgaccgg tacccattgc cccagccaca cctccgccca
      121 ctcgtaatag tagaagcggt cacccacccg tcgtacaccc gccacccgcc gcgcggggat
      181 gcccaccgaa cgcgacatcg tgacgaagag cgtgttcacg ccgtcgaaat cgcccttgct
      241 cgaggagaga ggcagcagcg cttcgggtct cagggccgtg gtgtcgtgcc gggcggtctg
      301 gaacacccag tgggtgatca tgccggcgac cttgacgggc tcccgttcct ggccgatcag
      361 ccgcatcaag gggaatccga cgtccatggt ggcgaccggc atcagaggct ccgagtgcag
      421 cgtctcggtg aagagcgagt cgtggagagg tacgcggtac cgtgggtgga ggctgtccag
      481 cgccacctgt cgaacggtca cggtatcacc ggccaacgcc tggccacccc ccgccagatc
      541 gaagccggca atcggcgctc cgccgaggcg gacgcgcatt gccgtcaacg gaggcatggg
      601 gcgttccggt ggaaaggtcc ccacgagccc gggccgctgg gacaacgatg ccgccttgat
      661 cgccagggtg tcgcggtggc ggaagttctg gaccgcgagc tcgaatgccg tgcggttgta
      721 caccacgccc gtcgggcgct cttcgcgcac cacgcgtccc tgcggatcga tccagagccg
      781 gaaggggcca ccggtcccgg aaccctcgat ccggaaggcg ggggtggtgt cgaagtgcac
      841 cggtacccag gtcttcgtcg tcgagtcgaa ctcggcgctg tcgggattca cgagcgtggt
      901 ctcggcggcg acccgcacgt gcacgtcccg ggtgatgagg atcggtgagt cgaagacgcg
      961 ggtgtcgaac gtggctccca ccttcatctc gcccccgaac gccaggcgca acggcatcag
     1021 ggacgggatc atcggggtgc ggtgggtcct ggtctcggaa cgggcggtga ggcggccgtc
     1081 ggacaccgcg agcttgatgg tgctgtcctg cgtgaagatt ccggcggccg tgtacgcacg
     1141 tccgtcttcg gtgtacttca cgtcgaccgt ttcgagccgg agcgcgcggg agagcttggc
     1201 ctccgaacgc gtggtcgtgc ggttgagatg cccgtccgcc gcggtctcga tcacctcggc
     1261 gtccaccacg cgaagtgaat cggtcagggt gtcgagggtc gtggaggcgt acccgacctg
     1321 gcggccaccg acgtcgacgc gatagtacgc ggcgccgggc tgcaccgaga gggccgcgtt
     1381 cgcgagccgc gatccggtgc tttggaagta cacacgccgg accagccagg ccatggacac
     1441 ggcccagatg atcagtatga ggatcccgac ctgcttgcgg ttcacgaggc gcgggcggaa
     1501 gcgcgacgca gtgcggcttc gatcacgtct ctctcatcgg tgttggcgac tacgaacgat
     1561 acctcagcgg ggaaggcgga gcgggcgcgg tgctgctcca ggacctcgac caggagatct
     1621 gcgacatcct tggccggcag ggcgcccgtg ccgtcgtcag cgccgaggat gggcgccgcg
     1681 acgtggcgga actcccactc cgaggcttgc tgcaacgccg cgcgccaggc gcggctcacc
     1741 atctcccgcg tcggtgcgtg atgagcctct tcccgaatgc aggcctgaat cacgaactcc
     1801 gctgcgacgg cgccctggcc ggcggtgacg accgcaccgc ccagcccgaa cgagtcggtc
     1861 acgagcgaat cgaagtgact gctggtgggg cgaacgacgg catcggcggg acaactggcg
     1921 aggtcggcca cgaccacgcg aatcaccggg cggtggcctc ccgcgctcgg cgatagtaca
     1981 cggtcgcttc ttccggacgg cggagccggt cgagcacgac gcccatgccg tagagggctc
     2041 gcgggtgctt ctcgtccacg gcgaggccct tctcatacgc caggcgcgca ccgtccaggt
     2101 catcgatgtg gttcagcgct tcacccaacc ggcaccagat cggcacgttg accggatcga
     2161 gctcgatcgc gtggcggaac tggggcactg cctgcgacca caggccgcgg cgcgcgaggg
     2221 tcacgcccgt ttccgcgagc gccatcgccg cgggaacggg cgtcgaggcg acgggagtgg
     2281 gagccgtcgg ttcgggagcg gcagctggag cgggtgccgg ctcgggcgct ggtggaggag
     2341 ccacaacggt gacgggcgcg gggacccgcg tcttcggcat gccaccggcg ggcgcgagcg
     2401 cgaggggacc ggtgagcaat ccgacgtctt cggtaaacgc agcggcctcc gccaggagtt
     2461 gagccgctac tcgggcgcgc tcggccaccg ggggccagcg gcgcagatgc cgtcgggtca
     2521 cgtcaccacc tcatgctcga tccaactcag gtacgcttcc agaccgtgct ccacggggag
     2581 cgccagcaac tcggggactt cataggggtg ccgagcttgt accgccgtca ccagagcatc
     2641 ccagcgtgac gcttcggtct tcatcagcac caccacttcc gtttcttcct tgatttcatt
     2701 cttccagcga tagatcgacg tccccgccgg cagaacagtc ccacaggcaa tcacgcgctc
     2761 gctcaccagg gcgtggacga ggtgccgcgc atcttcgacc gtgctcagcg tggtgagaac
     2821 gacgagagca ccggtcgcca taggggcagc taacgtacgg tttgcgagcg agtta
//
LOCUS       DASUBP010000290        22447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1046853, whole genome shotgun sequence.
ACCESSION   DASUBP010000290 DASUBP010000000
VERSION     DASUBP010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 22447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22447
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1046853"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..763
                     /locus_tag="VFU75_11040"
     CDS             <1..763
                     /locus_tag="VFU75_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467416.1"
                     /translation="TVFQRDRYAPPSGHRKPDLPSPQLVVPRLRMGRTGAIVIAVHVL
                     VLALLFWKSAELLAGSGDGPGPRGGGGGGGRQALHMFLLSGVNAPQAVAVPPAPQVTT
                     HLPLPVPAKLDLPKLQPAPQPVETQVATAPGTGNGTTGGPGEGPGSGGGKGAGVGPGV
                     GNDSGPGHGGGPHILIASPKLTILPPPCFHGRTVAMIAVSEGGEVTDVQLEPPPKDAG
                     CRETLVKQLKSNQFYPAKTLEGVPVASVVPFTISR"
     gene            812..2272
                     /locus_tag="VFU75_11045"
     CDS             812..2272
                     /locus_tag="VFU75_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU5467417.1"
                     /translation="MSLFARKTVDQCIADGESGGGLKRSLGAIQLTALGVGAIIGAGI
                     FVAIGSAITGDSAHPGAGSAIVISILVAGVTSALAAITYSEFAAMIPIAGSAYTYAYA
                     TLGELVAWIIGWDLILEYAVGNIAVAIGWSGYFDSLLRGLGITLPRWIVTDTLTAVNT
                     HAAVPHLLGIPVSFNLPAFLIVAVITWLLVIGTTESAWVNTLMVAVKVGIILLFLVVG
                     LGFIHTSQWTTPSFAPNGLRGIGAGAAIIFFAYIGFDAVSTASEEAKDPARGIPIGII
                     ASLIICTLLFVAIALVLTGLIPWDQLNVPDPLAVALHYVHADWATGILSLGAVAAMTS
                     VLLVFQLGQARIFMSMGRDGLLPPWAAKIHPKYKTPHITTIVTGIFVGVFAAFAPIGI
                     VLELTNIGTLFAFILVALGIIVLRRVDPERPRPFRTPWVPVLPAISIAFCVYLIWNLP
                     TITQLRFVGWLALGAVIYFLYSVRHSKVRGRLKDGA"
     gene            complement(2269..3192)
                     /locus_tag="VFU75_11050"
     CDS             complement(2269..3192)
                     /locus_tag="VFU75_11050"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HEU5467418.1"
                     /translation="MTSPIQPSSRLAVYVGVDAGGSHTTVVLADQARQVLARVEGPAS
                     AMRPGGGKKSAAVIADAVRRAANQAGRELPAGWMGVGAAGAGRDVEQEELQEALMETG
                     LAGEVRVVSDGEAALRSAFGTEPGMLISAGTGSIAYARDPGGRLHRAGGYGWQMGDEG
                     GGYWLGRQALAAAGAQRDGRGEGSTLGSRLLGALGLRDFDDLVRWAATATPGQVASLA
                     PHVLNAAQEGEQVAQMIVGEGAAALGDLVRALLTHFREGPVALATGGGLLRADSPLLT
                     ALRSNLRADPVALAHRPVDPALGALTLAPKP"
     gene            complement(3238..4674)
                     /locus_tag="VFU75_11055"
     CDS             complement(3238..4674)
                     /locus_tag="VFU75_11055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HEU5467419.1"
                     /translation="MRSLDVVVIAVYCAAVIAFGLIRAGRQRDAADYFLGHRGLPWWA
                     LMFSVVATETSALTVISVPGIAARGDLTFLQIGFGYLVGRIGVAALLLPGYFEGTQDT
                     AYQRLEHRFGPLARRVASGVFLGTRALADCVRIFATAIPLAIITHWSLPAGILAIGVV
                     TLVYTWVGGLKAVVWVDVIQLGVYLIGGIATLIVATQAAGGTAAFIGAANAGKLKFLD
                     FTPSFHLLYTFWGGVLGGALLSAASHGTDHLIVQRLLAARNLKDAQRALVGSGVLIIL
                     QFALFLLVGTSLWLAGADPGSVGRSDAIYPTFVVNRLPAGLSGLVVAGILAAAMSSHA
                     SAVNSLASATTHDFYVPLTGKTDPARVLSVGRWLTLFWTAVLVAGAMAFRDQNTPVVQ
                     LALSVASITYGGLLGTYILGGTSRPRQVDVIVAMIVSTLVMLPIVIGVPVRWLPGLAW
                     PWYVPLGTTITVAVGMLSSRIRRPPLDG"
     gene            complement(4759..5115)
                     /locus_tag="VFU75_11060"
     CDS             complement(4759..5115)
                     /locus_tag="VFU75_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HEU5467420.1"
                     /translation="MTTTQQTPVTVTLTDRAADEVRKFMAEEKVPAETAGLRIGVMPG
                     GCSGFKYSLNIEERAAADDITIESAGVRLFVDTFSAQYLNGVTLDYKSNFQESGFAFE
                     NPNATGGCGCGSSFTV"
     gene            complement(5177..6361)
                     /locus_tag="VFU75_11065"
     CDS             complement(5177..6361)
                     /locus_tag="VFU75_11065"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HEU5467421.1"
                     /translation="MRLHAALILAGAVACRPPAIPEPVSTGIPAASPASLGFDSGLLD
                     SVTIYLRTQVDSAFPGATVAIGRHGRMALVAAVGHYSTTDPRPVTTATLYDLASLTKV
                     VGWTTACMQLVDQGRLDLDAPVSRYVPEFRGPQKDQVKIRNLLTHTAGFPPDLPLWQD
                     TKTREQAFALADTAPLANPPGQTYVYSDVSAIIGEQVIERITGERLDQYLATHVFGPL
                     GMVATRYLPPASWKPMIAPTEIDTIMRHRLIWGTVHDESADRLGGVSGNAGLFSNALD
                     LSRFAAMLLAGGAWDSLTLVHAETIDEFTRRQDLPPGSSRALGWDTPSQPSSGGTKMT
                     DRAFGHTGFTGTSIWIDPGRDLFVILLTNRVNPTRANLRIAKVRPRVADLVGQALTHP
                     EY"
     gene            complement(6340..8112)
                     /locus_tag="VFU75_11070"
     CDS             complement(6340..8112)
                     /locus_tag="VFU75_11070"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HEU5467422.1"
                     /translation="MRAHRLLYLIAPLLLVACQPSRPPVAETPVPAPAAPSPKDPTDS
                     LIAAMSIHDKVGQLIVPWLPGNYTARDDSTFLVASRWVDSLRVGGIIISVGSPFDIAA
                     KLNALQSRSRLPLLIGADLEYGAGMRLVGGTSFPAIMAAGATGNPQDAYTIGRAAALE
                     GRAVGIHVDFAPDADLNNNPLNPIINTRSFGEDPAQVSKLVSAFVHGVQDNGMLATLK
                     HFPGHGNTGIDSHIGLPYIGAGYRQLDSLELIPFRAGIAAGVAVVMSAHIALPAITGS
                     DDPGTLSKAVLTGMLRDSLKFKGLVVTDALTMGAIVSKYGAGEATVRAFLAGSDLLLM
                     PADPDSAMTAMTQAVISGRITPARLDSSLHAVLETKYKLGLFSRRTVPLDSIMSVVGD
                     SAFQHQANDIAARALTLVRDTTGTLWSVRRRRSKIALIAYGDELNGSVGQTLTTELRT
                     GGDTIEYFRLWPMSGPASYDSARTVMTRAGTVIFTSVVRPLSGRGSIALPDSLARLIT
                     VTDSAKPTILISVGSPYLLDQVPTAHSYLLGWSDARAVERALGDALLGKTPIGGHLPI
                     RVPPYPVGWGITLAPGMPCDCMRH"
     gene            8128..9300
                     /locus_tag="VFU75_11075"
     CDS             8128..9300
                     /locus_tag="VFU75_11075"
                     /inference="COORDINATES: protein motif:HMM:NF018743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HEU5467423.1"
                     /translation="MSLAAAGLGSAQVRPGIEVVLTDSAHLINGRRIGLLSNQTGVDR
                     QGARDVDVLRAHGFQVAALFGPEHGFQGKEDRPGLPDAVDSATGLPIYSLYGGSEAKA
                     RAALATIDVLLIDLQDVGARYYTYPASATSLMRDATKLGKAVVVLDRPDPVGGDLVQG
                     NVRVKIGDPDSEFVGFLPVAMRHGMTLGELLRMANDQLGIHARLTVVPAAGWRRRMPY
                     DSTGLPWIKPSPNLPTLESAYHYPGLCLFEGTNVSVGRGTDFAFQVIGASWIDPVKLR
                     QALGGVRGVAVDTITFTPVHPTDGKDDGVPLRGLRFRVTDRRSYDPTRLAVRLLVLLH
                     RDYPSRFALRAAQFDRLAGAGLRARVEAGQSAAMITRQWDQGVRRFERERAKYLLY"
     gene            9362..10054
                     /locus_tag="VFU75_11080"
     CDS             9362..10054
                     /locus_tag="VFU75_11080"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEU5467424.1"
                     /translation="MILIPQSIRDGFLKAVGPIARGAIRAGLTPNIITTCGAAVVVGS
                     AVAYWQDFPRWGGALLLASGLFDILDGQMARLGNLSTKFGAFYDSTLDRVGESAVFLG
                     IAIWFIHGGVPAAQRDLAVGLAIVGLAASLLVSYTRARAEGLGLEAKVGIAQRAERFL
                     VLGAPTLFFGAGSGGQLIFWIMALLAVTTTITVIQRVVHVARITDPRGSGSTPRKRDT
                     LAGHPAALRKGQ"
     gene            10051..11388
                     /locus_tag="VFU75_11085"
     CDS             10051..11388
                     /locus_tag="VFU75_11085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HEU5467425.1"
                     /translation="MTVPAGGKREIAPAKGKLGVMLVGLGAVSTTFIAGVENIRQGHA
                     LPIGSLTQMGTIRLGKRTDKRAPKIKDFLPLADLKDLVFTAWDPIPDDAHTAAVKAGV
                     LERDHIEPIADFLKSIKPLPAAFDQNYVKRLHGTNVKRGKSKKDLAEQLRADIREFKK
                     SGGVSRVVIVWAASTEVFITPGPAHQTLAAFEKAMEQNDPAIAPSMLYAYAALMENVP
                     FANGAPNLCVDVPALAALAEERKIPIGGKDFKTGQTMMKTVLAPAFKARMLGLAGWYS
                     TNILGNRDGEVLDDPESFKTKEESKLGVLEYILQPDAYPELYGNVFHKVRINYYPPRG
                     DNKEGWDNIDIFGWLGYPMQIKVDFLCRDSILAAPIVLDLALFFDLAQRAGLGGVQEW
                     LSFYFKSPQTAPGLYPEHDLFIQQTKLKNTLRWLMGEEQITHLGVEYYEYGDK"
     gene            11457..13937
                     /locus_tag="VFU75_11090"
     CDS             11457..13937
                     /locus_tag="VFU75_11090"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF001950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HEU5467426.1"
                     /translation="MTITALLAFGMLLAFAPARALMAQGPAATTAAPAQHEAGGEAAM
                     ILPSLSQANFLGVDGHSLLVWGLVVCVLGLGFGLMIYTQLKNLPVHQSMREISELIYE
                     TCKTYLITQGKFLMILWVFIGAIAAIYFGGLATTVDASGAVVHGYPMGTVVIILIFSL
                     IGIAGSYGVAWFGIRVNTFANSRTAFGALSGKAFPCHDIPTKAGMSIGMLLVSVELIM
                     MLIILLFIPGNYAGPCFIGFAIGESLGAAALRVAGGIFTKIADIGSDLMKIVFKIKED
                     DARNPGVIADCTGDNAGDSVGPSADGFETYGVTGVALITFILLAVHDPLVQVQLLVWI
                     FAMRIIMIVTSGLSYFINAAITKAKYATAAKMDFEAPLTMLVWLTSVISIVATFIVSK
                     LMIPNLGNDPTLWWKLSLVITCGTLAGAIIPELVKVFTSMNSKHVAEVVTSSKQGGAS
                     LNILSGLVAGDFSAYWLGFAMLALMAIAYGVSTVGMSALMVAPAVFAFGLVAFGFLGM
                     GPVTIAVDSYGPVTDNAQSVFELSTIEQIPGVQAQIKKDFGFDVDFAKAKDNLEENDG
                     AGNTFKATAKPVLIGTAVVGATTMIFSIIVALTNGLDPQLVNNLSILHPPFLLGLIAG
                     GAMIYWFTGASTQAVTTGAYRAVEFIKANIKLEGATKASVEDSKKVVEICTQYAQKGM
                     FIIFLAVFFATLAFAFIEPYFFIGYLISIALFGLYQAIFMANAGGAWDNAKKIVETVL
                     KQKGTPLHDATVVGDTVGDPFKDTSSVAMNPIIKFTTLFGLLAVELAVSLSAERGTGL
                     TEAIAAVFFLLSVVFVWRSFYSMRIESQ"
     gene            13944..14369
                     /locus_tag="VFU75_11095"
     CDS             13944..14369
                     /locus_tag="VFU75_11095"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU5467427.1"
                     /translation="MTGVQLAVTILGGCAIGWVLWYFLGSRAAPAAARDADGVQEVRI
                     LVKGGYSPDTILVQAGKPVRLVFYRDETADCSERVVFEHFGIDQALPAFQNTTIEFTP
                     GEPGEFPFRCGMSMLKGRLVVEPADAERRAGPSPHPAHG"
     gene            14362..16908
                     /locus_tag="VFU75_11100"
     CDS             14362..16908
                     /locus_tag="VFU75_11100"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU5467428.1"
                     /translation="MAKARITLPIEGMSCASCAITVQEALAAQPGVSAASVNYATGRA
                     SVDYDDAGAGIGQMVRAVRNAGYDCGLANVTIAIVDLHYAPSVAPLEKALAQVPGVVK
                     VLANQALETASVEYVPGVTSAQDLEGAVRAAGFAVAEPIPSEDPEERERVARAFEVRA
                     LTWKFALAAVVAVIAMLGGMILMADRPLDTFHRFDLLGRVLMPVSERLRDLVQGRWPL
                     DSDWIRRVLGLLTIPVMVWSGGQFYRATWSGIRHRTADMNTLIAVGTGAAFLYSVVAT
                     VIPGVFLQAGLPADVYYEAVSAIIALILFGRLLEARAKGQTSAAIRKLAGLRPKTARV
                     RRGTAESDVPIEAVVVDDFVIIRPGERVPVDGIVTEGVSSVDESMLTGEPLPVTKKVG
                     DEIVGGTMNTTGSVVFRATRVGKDAALGQIIRMVEEAQGSKAAIQSLADRVAGVFVPV
                     VIAIAIAAFVVWFDLGPSPAVLFATVAFVTVLIIACPCALGLATPTAILVGTGRAAEL
                     GILIRGGQAIENLSRVKAILLDKTGTITEGHPVVTHILTAKRSDGAPVSPADVLKWAA
                     SVEQRSEHPLALAILDAAKAKGVSMLAVDKFAAMEGRGVRGTVDRRIVEVISLRHARE
                     RSLDLGGLANDADRLAGEGRSPVIVVVNNTVYAIIAIADPIKSGARDAVAALLARGVE
                     VTMVSGDSKRAAEAVAKEVGIDDVVAEVLPSQKADLVRKFQRKGRKVAMVGDGINDAP
                     ALAQADVGIAIGTGADVAMEASDVTLIRGDLRAVDTALALSRKTLSTIRRNLFWAFIY
                     NVVGIPIAAGILYPVTGLLLSPVIASAAMAWSSLSVVLSSLTLRRFEPAA"
     gene            16922..17212
                     /locus_tag="VFU75_11105"
     CDS             16922..17212
                     /locus_tag="VFU75_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467429.1"
                     /translation="MRYFHRTHLSPDEVLAAAQSYFGGRLAPSEETNRRRSFAGALGK
                     VSVAVKAEGGHYTFIDVTTDQVGEAELDKVAKRFLALVHQKADPSHQVRGAY"
     gene            complement(17209..17430)
                     /locus_tag="VFU75_11110"
     CDS             complement(17209..17430)
                     /locus_tag="VFU75_11110"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEU5467430.1"
                     /translation="MLSTTLSVSGMTCGHCQAKVEKALRGVDGVFGAEVDLPGGTAQV
                     EAGDGVTNEMLIDAVKRAGYRATVAAETA"
     gene            17581..18552
                     /locus_tag="VFU75_11115"
     CDS             17581..18552
                     /locus_tag="VFU75_11115"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU5467431.1"
                     /translation="MRLTRTLEERLTALYRQSKVIGGLFRSLGQEGESVASAYALERG
                     RNRDFLSPLIRNLGSLLVMGGKPVQILRQYMAKDQSPTRGRELNVHFGDLEMGFLGQI
                     SHLGDMVPVMAGITLTFKMRREPRVGLVYIGDGATSTGAFHEGLNFAAVQRCPLVVIG
                     EYNGYAYSTTPERQFAVKNLADKAKGYGIPAVTVDGNDVLAVYEATKYAVDRARMGKG
                     VHFVEVKTFRMKGHAEHDDQHYVAKADLERWAKNDPIDRYWNQLLTSGWAEEQDLKDI
                     DSDVRDEIDQATDACVNEPLPPAESALTAVYADPPAAERLWYRGLNG"
     gene            18545..19522
                     /locus_tag="VFU75_11120"
     CDS             18545..19522
                     /locus_tag="VFU75_11120"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEU5467432.1"
                     /translation="MAEVTFLEGLRQGLREEMERDERVFILGEDVGAYGGAFKVTEGF
                     QKQFGESRVIDTPISEAAIVGAAAGAAHLGMRPVAEMQFIDFISCAYDMLTNYVATAR
                     YRAGLPTPMVVRGPCGGYVRGGPFHSQNPEAAFFHTPGLKIVYPSTVRDAKGLIKAAI
                     RDDDPVLYLEHKWLYRRIKDQIPEGEEIITPIGSARIAREGKDLSIITYGATVWKSLE
                     AAEVLEKDGVSVEILDLRTLLPLDDDAILKTVKKTNKVLIVHEDTRTGGLAGEITARI
                     NEKAFEWLDGPIMRVTAHDVPIPYAPTLEDFVLPQTDDIVKAARWLAGY"
     gene            19534..20889
                     /gene="sucB"
                     /locus_tag="VFU75_11125"
     CDS             19534..20889
                     /gene="sucB"
                     /locus_tag="VFU75_11125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase, E2 component,
                     dihydrolipoamide succinyltransferase"
                     /protein_id="HEU5467433.1"
                     /translation="MARVDVLMPQMGESIAEGTLSKWLKKVGDDVKRDEPLFEISTDK
                     VDAEIPAPTAGVLAEIKVQQGQTVPVQTLVAVIETEKGAAASAPAPAPAAPAPAAKAA
                     PAPAPAPKAAPAPAAAAPMAPPRAPAPAPAAVPANGDETAEDRLRRRSTPLVRKIAAE
                     HQVDISGISGTGYAGRVTKQDILGYIERGVPAGAPSAPRLPASAPSGPIEHPAVEPWP
                     GDRVEPWSKIRKITADHMIMSKRVSAHVASFFEVDFTRVSQLRAKAKASYQERGVNLT
                     FLAFIAKAVAENLRKHPVVNAAVSGDNTIYRRDINLGIAVALDWGLIVPVVKHADELS
                     LMGIARALNDLGERARTKRLTPDEIQRGTFTITNPGIFGDLIGIPIINQPQVAILGVG
                     AIEKKPRVITQPDGTDTIAIRTMGMLSMSYDHRVVDGADGDKFLADVKKMLQEFPEGA
                     V"
     gene            20892..21206
                     /locus_tag="VFU75_11130"
     CDS             20892..21206
                     /locus_tag="VFU75_11130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467434.1"
                     /translation="MAKVLTAARVRVPASNERDYLATLRDLCQFADARGQRIWVFRNA
                     KDPQLFIEFSESPTEMSHRAQASRLPEEIKLEKKLQNLVTYAPDAWELWSEVPLTAAA
                     EA"
     gene            21206..21481
                     /locus_tag="VFU75_11135"
     CDS             21206..21481
                     /locus_tag="VFU75_11135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467435.1"
                     /translation="MPQARGTLTVDGETWEYAPSGYDTVYTKDQFGVVFQCGTGSARR
                     RRFTRFTPMGSRNPEAALSELSDRQLLELFRQSQPAWTAPESAYGAR"
     gene            21483..22304
                     /locus_tag="VFU75_11140"
     CDS             21483..22304
                     /locus_tag="VFU75_11140"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEU5467436.1"
                     /translation="MHCHSTASDGECSPEEVAARAHEANLVAIALTDHDTLDGVGAAT
                     AAAAPLGVRIVPGCEFSVKAPWGEMHLLGYFLTPGHQALESFLVETREARKRRGHEMV
                     ERLKRAGVPIDLADVESEVKDGALGRPHVARALIRRGAADDFSDAFARFLGRGKPGYV
                     PKPLPSLDTVTGLIHQVGGVAVAAHLGEHGTEPQLRQFQAAGLDGVEIRHPRHSPAAE
                     ARLGRLAEKLGLAVSGGSDWHGDGDEGASHAQLGGMDVPLEWLERLENRRGKTSR"
BASE COUNT         3680 a         7413 c         7641 g         3713 t
ORIGIN      
        1 gaccgtgttc cagcgcgacc gttacgcccc tccgtccggg caccgcaagc ccgatctccc
       61 gtcaccgcag ctcgtcgttc cccggctgcg gatggggcgg acgggtgcga tcgtcatcgc
      121 cgttcacgtc ctcgttctcg ccttgctctt ctggaagagc gccgagctcc tggccgggtc
      181 gggggatggc ccggggccgc gaggcggggg cggcggaggc ggacgccagg cgctccacat
      241 gtttctgttg tcgggggtca atgccccgca agcggtggcc gttccgccgg cgccacaggt
      301 caccacgcat ctcccactcc ccgtgccggc gaagctcgat ctgccgaagc tccagcccgc
      361 gcctcagccg gtcgagactc aggtcgcgac tgcaccagga acgggcaatg ggaccaccgg
      421 gggtccggga gaaggcccgg ggtcgggtgg agggaagggt gcgggcgtgg gtcccggtgt
      481 cggcaacgac tcgggtccgg gccacggggg cggtccccac atcttgatag cgtctccgaa
      541 gttgaccatt ttgccgccgc cctgcttcca cggacgcacc gttgccatga tcgcggtgtc
      601 ggagggggga gaggtgaccg acgtgcagtt ggagccgccg ccgaaggacg ccggttgccg
      661 ggaaacgctg gtgaagcagc ttaagtcgaa ccaattctat cccgcgaaga cactcgaagg
      721 cgttcccgtc gcgagtgtgg tcccgttcac gatctcgcgg taaccgacag tcaacacagg
      781 caggcggcga tgccgccccc cttggaggcg catgtcgctc tttgcccgta agacggtcga
      841 ccagtgcatc gccgacgggg aatccggggg cgggctcaaa cgctcgttgg gcgccatcca
      901 gctgaccgcg ctcggcgtcg gcgccatcat cggcgccggg atcttcgtcg ccatcggctc
      961 ggccattacc ggggacagtg cccaccccgg ggcgggctcg gcgatcgtca tctcgattct
     1021 cgtcgccggc gtcaccagcg ccctcgcggc catcacgtat tcagagttcg ccgcgatgat
     1081 cccgatcgcg ggcagtgcct acacctacgc ctacgcgacc ctcggcgagc tggtcgcgtg
     1141 gatcatcggg tgggacctga tcctcgagta cgcggtgggg aacatcgcgg tggccatcgg
     1201 ctggtccggc tatttcgatt cactgctccg cgggctgggc atcacgctgc cacgatggat
     1261 cgtgaccgac acgctcacgg cggtcaacac ccacgccgcg gtcccgcacc tgctcggcat
     1321 ccccgtctcc ttcaatctgc cggccttcct gatcgtggcg gtgatcacct ggctgctcgt
     1381 gatcggcacc acggagtctg cgtgggtgaa caccctcatg gtggccgtga aagtcgggat
     1441 cattctactg ttcctggtcg tcgggctggg gttcatccac acgtcccaat ggaccacgcc
     1501 gagctttgcc cccaacggac tgagggggat cggcgccggc gcggccatca tcttcttcgc
     1561 gtacatcggc ttcgacgccg tgagtaccgc gtcggaggaa gcgaaggacc cggcgcgggg
     1621 aattcccatc ggcatcatcg catccctgat catctgcacc ctgctctttg tagccatcgc
     1681 gctggtgctg accgggctca tcccctggga tcagctcaat gtccctgatc cgctcgcggt
     1741 ggcgctgcac tacgtgcacg ccgactgggc cacgggcatc ctgtcgctgg gagccgtcgc
     1801 ggccatgacg tcggtgctgc tggtcttcca gctcgggcaa gcgcgcatct tcatgtcgat
     1861 ggggcgcgac gggctgctgc caccctgggc cgccaagatt cacccgaagt acaagacgcc
     1921 gcacatcacc acgatcgtga cgggcatctt cgtcggcgtc ttcgccgcct tcgcacccat
     1981 cggcatcgtg ctggagctca cgaacatcgg cacgctgttc gcgttcatcc tggtggcgct
     2041 ggggatcatc gtgctgcgcc gcgtcgaccc cgaacgcccg cgcccgttcc ggacgccctg
     2101 ggtcccggtc ttgccggcga tatccatcgc gttttgcgtg tacttgatct ggaacctgcc
     2161 gacgatcacc cagctgcggt tcgtggggtg gctggccctg ggggcggtga tctacttcct
     2221 ctacagcgtc cgccacagca aggtccgcgg gcggctgaag gacggagcct agggcttcgg
     2281 cgccagcgtc agggcgccga gcgcgggatc caccgggcgg tgggccagtg cgaccgggtc
     2341 ggcgcggagg ttcgagcgca gggcggtgag caggggagag tcagcgcgca gcaacccgcc
     2401 acccgtggcc agagcgacag gtccctcccg gaaatgtgtc agcagggccc gcacgaggtc
     2461 acccagtgcg gccgcacctt ccccgacgat catctgcgcc acctgttcgc cttcctgtgc
     2521 ggcgttgagc acgtgcgggg cgaggctcgc cacctgtccg ggcgtcgctg tcgccgccca
     2581 ccgcaccaga tcgtcgaaat cccgaagtcc cagcgctccg aggagtctgg agccgagggt
     2641 cgagccctct ccgcgcccat cccgctgggc gcccgcggcg gcgagcgcct gccgtccgag
     2701 ccagtacccg ccaccttcat cgcccatctg ccacccgtac ccgccggcgc ggtgcagccg
     2761 cccgccggga tcgcgcgcat atgcgatcga ccccgtgccg gcggagatga gcatgccggg
     2821 ttcggtgccg aacgcggagc gaagcgccgc ctcgccgtcg gagacgaccc gcacttcgcc
     2881 cgcgagtccg gtctccatca gggcttcctg gagctcttcc tgctcgacat cgcgccccgc
     2941 accggctgcg ccgacgccca tccagccggc cgggagctcg cggcccgcct gattcgccgc
     3001 ccgccggacc gcgtccgcga tgacggccgc ggacttcttc ccgccccccg ggcgcatggc
     3061 ggaggccgga ccttccaccc gggcgaggac ctgccgggcc tggtccgcca gcaccacggt
     3121 ggtgtgagaa ccgccggcgt cgaccccgac gtagacggct agacggctag acggttggat
     3181 aggcgaggtc atcgcaggtc gaatctccct ttcgtgcgcc actagccgtc taaccgtcta
     3241 gccgtctagt gggggccgtc taatgcgcga ggacagcata cccacggcca cggtgatcgt
     3301 cgtccccagc gggacatacc agggccacgc gagtcccggc agccagcgta cggggacccc
     3361 gatgacgatg ggcagcatca ccagcgtact gacgatcatc gcgacgatca cgtctacctg
     3421 acgcggccgc gacgtgcccc cgagaatgta ggtgcccagc aggccaccgt aggtgatcga
     3481 cgccacggag agcgcgagct ggacgacggg cgtgttctgg tcgcggaagg ccatcgcgcc
     3541 ggcgaccagc accgcggtcc agaagagggt cagccagcgg ccgacggaca gcacgcgagc
     3601 aggatcggtc tttccggtga ggggcacgta gaagtcgtga gtggtggccg aggcgagcga
     3661 gttgaccgcc gaggcgtggc tgctcatggc cgcggcgagg atccccgcca cgacgagccc
     3721 cgagagaccc gccggcagcc ggttgacgac gaacgtcggg tagatcgcgt cactccggcc
     3781 gacgctcccc gggtccgcgc cggcgagcca caggctggtc ccaacgagca ggaagagcgc
     3841 gaactgcagg atgatcagga cccccgaacc cacgagtgcg cgctgcgcgt ccttgaggtt
     3901 ccgggcggcg aggagacgct gaacgatgag gtgatcggtc ccgtgagagg ctgccgacaa
     3961 cagggcgccc cccaggaccc cgccccagaa ggtgtagagc aggtggaacg agggcgtgaa
     4021 gtcgaggaat ttgagcttgc cggcattcgc ggcgccgatg aacgcggccg tgccccccgc
     4081 ggcctgcgtg gcgacgatca aggtcgcgat cccgccgatc aggtagacgc ccagttgaat
     4141 cacatcgacc cacacgaccg ccttgagtcc tcccacccac gtgtacacga gcgtgacgac
     4201 cccgatggcg aggatccccg cgggcaggct ccagtgcgtg atgatggcga ggggaatggc
     4261 ggtggcgaag atccggacgc agtcggccag cgcccgcgtg ccgagaaaca ctccggaggc
     4321 cacccggcgg gcgaggggcc cgaaccggtg ctccagccgc tggtaggctg tgtcctgggt
     4381 tccctcgaag tagccaggga ggaggagcgc cgcgaccccg atccgcccga cgagataccc
     4441 gaaaccgatc tggaggaacg tgaggtcgcc ccgggccgcg atgccgggaa cggagatcac
     4501 ggtgagcgcc gacgtctcgg tcgcgacgac ggaaaacatc agggcccacc aggggagccc
     4561 ccgatggccg aggaagtagt ccgcggcgtc ccgctggcgg ccggcccgga tcaggccgaa
     4621 tgcgatcacc gccgcgcaat agaccgctat gacgactacg tcgagggagc gcatgacggg
     4681 gcggggtggg gagcgaagaa aagaggcccg cgaaattcgc gggccccata tcttgtcagg
     4741 agtcgacgca ccaaagcgtc agacggtgaa cgacgatccg cagccgcagc cgccggtcgc
     4801 attcggattc tcgaacgcga accccgactc ctggaagttg ctcttgtagt ccagcgtgac
     4861 gccgttcagg tactgcgcgc tgaacgtgtc cacgaagaga cgcacaccgg ccgactcgat
     4921 ggtgatgtcg tccgccgccg cccgctcttc gatgttgagg ctgtacttga agccggagca
     4981 tccgcccggc atcacgccga tgcggagtcc cgccgtttcc gccggcacct tttcctcggc
     5041 catgaacttc cgtacttcat cggccgcccg gtccgtcagc gtgacggtga ccggcgtctg
     5101 ttgcgtcgtg gtcatctcgg tgcttcctcc ttgtcctgcc gcaacttagg aaggcggcac
     5161 gggtgtgtca acgtcctcag tactccggat gcgtcagtgc ctgccccacc agatccgcca
     5221 cgcgcggccg caccttcgcg atccggagat tcgcccgggt ggggttcacc cggttggtca
     5281 ggagaatcac gaacaggtcc cggcccggat cgatccagat cgaggtgccg gtgaaccccg
     5341 tatgaccgaa cgcgcggtcc gtcattttgg ttcccccgga cgacggctgc gacggcgtgt
     5401 cccagcccag agcccgggac gaccccggcg gcaaatcctg cctccgcgtg aattcatcga
     5461 tcgtctcggc gtgtaccagg gtcagcgagt cccaggcacc gccggcgagc agcatggccg
     5521 cgaagcggga gagatcgagc gcgttggaga agagccccgc gttccccgaa acgccgccca
     5581 gccggtcggc actttcgtca tggacggtac cccagatgag ccggtgacgc atgatcgtgt
     5641 cgatctcggt cggcgcgatc atcggcttcc agctggcggg cgggagatac cgcgtcgcca
     5701 ccatccccag gggcccgaag acgtgcgtcg ccaggtactg gtcgagccgt tcgccggtga
     5761 tgcgctcgat gacttgctcc ccgatgatcg cactcacgtc cgagtacacg taggtctgcc
     5821 ccggaggatt cgcgaggggt gcggtgtcgg ccagcgcgaa cgcctgctcg cgcgtcttcg
     5881 tgtcctgcca caggggaaga tcgggcggga aaccggccgt gtgggtcagc agattccgga
     5941 tcttcacctg atccttctga ggcccgcgaa actcgggcac gtaccgggac accggtgcgt
     6001 cgaggtcgag gcgtccctgg tcgaccagct gcatgcaggc ggtggtccag ccgacgacct
     6061 tggtcaacga cgcgagatca tacagggtgg ccgtcgtcac cggccgtgga tcggtggtgg
     6121 agtagtggcc gaccgcggcc accagcgcca tccgtccatg acgcccgatg gcgaccgtcg
     6181 cgcccgggaa ggcgctgtcc acttgggtgc gcaaatagat cgtgacgctg tcgaggagcc
     6241 cgctgtcgaa cccgaggctc gccggggacg cggcagggat cccggtgctc accggttcgg
     6301 gaatcgccgg cgggcggcac gcgacggcgc ccgcgaggat cagtgccgca tgcagtcgca
     6361 tggcatcccc ggtgcgagcg tgattcccca accgaccgga tagggcggta cccgaatagg
     6421 caggtgtcct ccgatgggtg tcttcccgag cagggcgtct cccaacgccc gctcgaccgc
     6481 tcgggcatca ctccacccca ggaggtagga gtgggccgtc ggaacctgat cgagaaggta
     6541 cggactgccg accgagatga ggatggtcgg cttcgcggaa tcggtcaccg tgatgagtct
     6601 cgcgagggaa tcagggagcg cgatcgagcc gcgccccgac aggggccgca cgaccgacgt
     6661 gaagatgacg gtgcccgcgc gagtcatcac cgtgcgcgcg gagtcgtagc tggccgggcc
     6721 ggacatcggc cagagacgga aatattcgat ggtgtctcca ccggttctga gctcggtggt
     6781 cagcgtctgt ccgaccgatc cgttcaactc atcgccgtac gcgatgaggg cgatcttgga
     6841 acgccggcgc cgcaccgacc acagggtccc cgtggtgtcc cgcaccagcg tgagcgcccg
     6901 ggccgcgatg tcattcgcct ggtgctgaaa ggccgagtcg cccacgacgc tcatgatcga
     6961 gtcgagcggc accgtgcggc gggagaacag gccgagcttg tacttggtct cgagcaccgc
     7021 gtgcagcgac gagtccaggc gcgcgggggt gatgcgtccg gagatcacgg cttgcgtcat
     7081 cgccgtcatc gcggagtcgg ggtccgcggg catgaggagg aggtccgacc ccgccaggaa
     7141 ggcgcgcacg gtcgcttccc ccgcgccgta cttgctcacg atggccccca tcgtcagcgc
     7201 gtcggtgacg accaggccct tgaacttgag cgagtcgcgc agcatcccgg tgagcacggc
     7261 cttggacagc gtgcccgggt cgtccgagcc cgtgatggcg ggcagcgcga tgtgcgcgct
     7321 catcaccacc gcgacgcccg ccgctatccc ggcgcggaag gggatgagct cgagggaatc
     7381 gagttgccgg tagccggcgc cgatgtacgg caggccgatg tgcgaatcga tgccggtgtt
     7441 cccatgcccg gggaaatgct tgagcgtggc gagcatcccg ttgtcctgca cgccatgcac
     7501 gaaggcggag acgagcttgg agacctgggc cggatcctcg ccgaacgacc gcgtattgat
     7561 gatggggttg agcggattgt tgttcaggtc ggcgtcgggg gcgaaatcca cgtggatgcc
     7621 cacggcccgg ccttcgagcg ccgcggcacg gccgatggta taggcatcct gaggattgcc
     7681 ggtggcgccc gcggccatga tcgccgggaa ggaggtgccc cccacgagcc gcatgccggc
     7741 gccatattcg aggtccgcgc cgatgaggag cggaagccgg gaccggctct gcagggcgtt
     7801 gagcttggcg gcgatgtcga agggtgagcc gaccgaaatg atgatgccgc cgacgcggag
     7861 ggaatcgacc caccggctgg cgaccaggaa cgtcgagtcg tcccgggcgg tgtaattccc
     7921 ggggagccac ggaacgatga gctggccgac cttgtcgtgg atcgacatgg cggcgatcag
     7981 cgaatcggtg ggatccttgg gggacggggc cgccggcgcc ggcaccggtg tctccgcaac
     8041 cggcggccgg ctgggctgac aggcgacgag caacagcggg gcgatcaggt acaggagtct
     8101 atgcgcgcgc atcatctctc aatcgtcttg agtctcgccg cggccggcct cgggtcggcc
     8161 caggtgcggc cgggcatcga agtagtgctc accgattccg cccatctcat caacgggcgc
     8221 cgtatcggcc tgctctccaa ccagaccggc gtggaccggc aaggcgcgcg ggacgtcgac
     8281 gtcctgcggg cacatggatt ccaggtcgcc gccctcttcg gtccggaaca tggctttcag
     8341 ggcaaggaag acaggccggg ccttcccgac gcagtcgact cggccacggg acttccaatc
     8401 tatagcctct acgggggctc ggaggcgaag gcccgcgcgg ccctcgcgac catcgacgtc
     8461 ctgctcatcg acctccagga tgtcggtgcc cggtattaca cctacccggc cagtgcgacg
     8521 tcattgatgc gggatgcgac gaagctcggc aaggcggtcg tcgtcctcga tcgccccgat
     8581 cccgtcggag gcgacctggt gcaggggaac gtacgggtga aaatcggtga tcccgactcc
     8641 gagttcgtcg gatttcttcc ggtggcgatg cggcacggga tgacgctggg ggaattgctg
     8701 aggatggcaa atgaccagct gggcatccac gcgcgcctca cggtcgttcc cgccgcgggg
     8761 tggcgccggc gcatgccgta cgattccacc gggctgccgt ggatcaaacc gtcccccaac
     8821 cttcccaccc tcgagtccgc ctatcattat cccgggctct gcctgttcga gggcaccaat
     8881 gtctcggttg ggcgcgggac ggacttcgcc ttccaggtga tcggcgcctc ctggatcgac
     8941 cccgtgaagt tgcggcaggc gctcggcggc gtccgcggag ttgcggtgga taccatcacg
     9001 ttcaccccgg tccacccgac ggacgggaag gatgacgggg tgccattgcg cgggctgcgg
     9061 tttcgcgtga cggaccggcg ctcctatgac ccgactcgcc tggcggtccg tctcctggta
     9121 ctgctgcatc gggactaccc gtcccggttc gccctccgcg ctgcccagtt cgaccggctg
     9181 gcgggcgcgg gcctgcgggc gcgcgtggag gcggggcagt cggcggcgat gatcacgcgc
     9241 cagtgggatc agggagtccg tcggtttgaa agggagcggg ccaagtactt gctttattga
     9301 gggttctgtc tccttcccct ccttccatcc ccctccggcc ttcattacct tccccccggc
     9361 catgatcctt attccccagt cgatccgaga cggattcctg aaagccgtcg gaccgattgc
     9421 ccggggtgcc atccgggcag gcctcacgcc caacatcatc accacctgtg gagcggccgt
     9481 cgtcgtcggc tcagccgtcg cgtactggca ggattttccg cggtggggtg gggcgctgct
     9541 gttggccagc gggctcttcg acatcctcga cgggcagatg gcccggctcg ggaatctgtc
     9601 caccaagttc ggtgcgttct acgactcgac actggaccgg gtgggtgagt cggcggtgtt
     9661 tctcgggatc gccatctggt tcatccacgg cggcgtgccc gccgcccagc gggacctcgc
     9721 ggtgggactc gccatcgtgg gtctcgcggc cagcctcctg gtgtcgtaca cgcgggctcg
     9781 cgccgaggga ctcggtctcg aggcgaaggt gggaatcgcg cagcgggccg agcggtttct
     9841 ggtgttggga gcgccgacgt tgttcttcgg agccgggtcc ggcggccagc tgatcttctg
     9901 gatcatggcg ctcctggcgg tgacgaccac gatcacggtg attcagcggg tggtgcacgt
     9961 ggcgcggatc acggatccgc gaggctcggg ctccacgccg cgcaaacgcg atacgctcgc
    10021 ggggcatccc gcggcgctgc ggaaaggaca gtgacggtgc cggcaggggg gaagcgcgag
    10081 atcgcgcccg cgaagggcaa gctgggagtg atgctggtgg gcctcggggc cgtctcgacc
    10141 acgttcatcg cgggggtcga gaacatccgg cagggtcacg ccctgccgat cgggtccctc
    10201 acgcagatgg ggacgatccg gctcggcaag cggaccgaca agcgtgcccc gaagatcaag
    10261 gactttctcc cgctcgcgga cctgaaggac ctggtgttca cggcgtggga cccgatcccc
    10321 gatgacgcgc acacggcggc ggtgaaggcc ggtgtgctgg agcgggacca catcgagccg
    10381 atcgcggatt tcctgaaatc gatcaagccg ctcccggccg ccttcgacca gaactacgtc
    10441 aagcggctcc acggcaccaa cgtgaagcgc gggaagagca agaaggacct ggccgagcag
    10501 ctgcgcgccg acatccgcga gttcaagaag tcgggtggcg tgtcacgcgt cgtgatcgtg
    10561 tgggcggcgt ccaccgaggt gttcatcacc ccgggcccgg cgcatcagac cctggcggcg
    10621 ttcgagaagg cgatggagca gaacgatccg gccatcgcgc cgtcgatgct ctatgcctac
    10681 gcggccctga tggaaaacgt gcccttcgcg aacggcgcgc ccaatctctg cgtcgacgtg
    10741 cccgcgctcg ccgcgctcgc tgaagagcgg aagattccca tcggcggcaa ggacttcaag
    10801 acgggccaga ccatgatgaa gaccgtgctg gcgccggcct tcaaggcgcg catgctgggt
    10861 ctcgcgggct ggtattcgac gaacatcctg gggaatcgcg acggggaagt gctcgacgac
    10921 ccggagtcgt tcaagacgaa ggaagaatcg aagctgggcg tgctcgagta catcctccag
    10981 cccgacgcct atcccgagct ctatgggaac gtcttccaca aggtccgcat caactactat
    11041 ccgccccgcg gcgacaacaa agaaggctgg gacaacatcg acatcttcgg gtggctgggc
    11101 tacccgatgc agatcaaggt cgatttcctg tgccgggact cgattctcgc cgcgcccatc
    11161 gtgctcgacc tcgcgctctt cttcgatctg gcgcagcggg cggggctggg cggcgtgcag
    11221 gagtggctct ccttctactt caagagcccg cagaccgcgc cggggctgta cccggagcac
    11281 gatctcttca tccagcagac gaagctgaag aacactctcc ggtggctgat gggtgaggag
    11341 cagatcaccc acctgggagt cgaatactac gagtacgggg acaagtgagc cgccgtaccc
    11401 aaccgctttc gcacacagga gatgaccgtc acatgacgtt cgagcgcatt cgccgaatga
    11461 cgatcaccgc ccttttggcg ttcggaatgc tgctggcgtt cgcgccggcc cgcgcgctga
    11521 tggcccaggg tcccgccgcc acgacggccg caccggctca gcatgaagcg ggcggcgagg
    11581 ccgccatgat tctccccagc ttgagtcagg ccaacttcct gggcgtcgac ggccactcgc
    11641 tcctcgtctg ggggctggtg gtctgcgtgc tgggtctcgg gttcgggctg atgatctaca
    11701 cgcagctcaa gaacctcccg gtacaccagt cgatgcggga gatttcggag ctcatctacg
    11761 aaacctgcaa gacctacctg atcacgcagg gcaagttcct gatgatcctc tgggtgttca
    11821 tcggagccat cgccgcgatc tacttcggcg ggctcgcgac caccgtggac gccagtggcg
    11881 ccgtggtcca cgggtatccc atgggcacgg tcgtcatcat tctcatcttc agcctcatcg
    11941 ggatcgcggg gtcgtacggc gtggcctggt tcggcatccg ggtgaacacg ttcgccaact
    12001 cgcggacggc gttcggcgcg ctgagcggga aggcgttccc ctgccatgac attccgacca
    12061 aggcggggat gagcatcggc atgctcctgg tgtcggtgga gctgatcatg atgctgatca
    12121 tcctcctctt catccccggc aattacgcgg gtccctgctt catcgggttc gcgatcggcg
    12181 agtcgctggg cgcggcggcc ctgcgcgtgg cgggcggcat cttcacgaag atcgcggaca
    12241 tcggttccga tctcatgaag atcgtgttca agatcaagga agatgacgcc cggaacccgg
    12301 gcgtgatcgc ggactgcacc ggcgacaacg ccggcgattc ggtcggcccc tcggcggacg
    12361 gcttcgagac ttacggcgtc acgggcgtcg cgctcatcac cttcattctg ctggcggtgc
    12421 acgatccgct ggtgcaggtc cagcttctcg tctggatctt cgcgatgcgc atcatcatga
    12481 tcgtgacctc cggtctctcg tacttcatca acgccgcgat caccaaggcc aagtacgcca
    12541 ccgcggcgaa gatggacttc gaggcgccgc tcacgatgct ggtgtggctg acgtcggtca
    12601 tctcgatcgt cgcgacgttc atcgtgtcga agctgatgat cccgaacctc gggaacgacc
    12661 cgacgctgtg gtggaagctc tcgctggtga tcacctgcgg cacgctcgcg ggcgccatca
    12721 tcccggagct ggtcaaggtg ttcacctcga tgaactcgaa gcacgtcgcc gaggtcgtca
    12781 cgtcctccaa gcagggcggg gcgtcgctca acattctctc cggtctcgtg gcgggtgact
    12841 tctcggccta ctggctcggc ttcgcgatgc tggccctgat ggcgatcgcg tacggcgtct
    12901 cgacggtggg aatgtcggcg ttgatggtgg cgccggccgt gttcgcgttc ggcctcgtgg
    12961 ccttcggatt cctgggcatg ggcccggtga ccatcgccgt cgactcctac ggcccggtga
    13021 ccgacaacgc ccagtcggtg ttcgagcttt cgaccatcga gcagatcccg ggtgtgcagg
    13081 cgcagatcaa gaaggatttc ggcttcgacg tggacttcgc gaaagcgaag gacaatctcg
    13141 aggagaacga cggcgcgggg aacaccttca aggccaccgc caagccggtg ctcatcggca
    13201 ccgcggtcgt gggcgctacg accatgatct tctcgatcat cgtggccctc accaacggcc
    13261 tggatccgca gctggtgaac aacctctcga tccttcaccc gcccttcctg ctcggcctca
    13321 tcgcgggcgg cgcgatgatc tactggttca cgggcgcatc cacccaggcc gtgaccaccg
    13381 gcgcctaccg cgcggtggag ttcatcaagg cgaacatcaa gctcgagggc gccaccaagg
    13441 cgtcggtgga agactcgaag aaggtggtcg agatctgcac ccagtacgcg cagaagggca
    13501 tgttcatcat cttcctggcg gtcttcttcg ccacgctcgc cttcgccttc atcgaaccct
    13561 atttcttcat cggatatctg atctccatcg cgctcttcgg tctgtaccag gccattttca
    13621 tggccaatgc cggcggcgcc tgggacaacg cgaagaagat cgtcgaaacg gtgctgaagc
    13681 agaaaggcac gccgctccac gacgccaccg tcgtgggcga caccgtcggc gatccgttca
    13741 aggacacgtc gtccgtggcc atgaacccca tcatcaagtt cacgacgctg ttcggtctcc
    13801 tcgcggtgga gctggccgtc tcgctctccg ccgagcgtgg gacgggcctg acggaagcga
    13861 tcgcggcggt gttcttcctg ctctccgtgg tcttcgtctg gcggtcgttc tacagcatgc
    13921 gcatcgagtc gcagtaacca cgcatgaccg gggtgcagct cgccgtcacc atactcggcg
    13981 gatgcgccat cgggtgggtg ctgtggtact tcctcggatc acgggcggcc cctgcggccg
    14041 cccgtgatgc cgatggggtg caggaggtcc gcatcctggt gaaaggcggc tactcgcccg
    14101 acaccatcct cgtccaggcc ggcaagcccg tgcgcctggt cttctaccgc gacgaaaccg
    14161 ccgactgctc cgagcgcgtc gtcttcgagc acttcggcat cgatcaggcc cttccggcat
    14221 ttcagaacac gaccatcgaa ttcacgcctg gggaacccgg cgagttcccc ttccgttgtg
    14281 ggatgagcat gctgaaaggc cgtctggtcg tcgagccggc ggatgccgaa cgccgcgccg
    14341 gaccttcacc gcaccccgcc catggctaaa gcgcggatca ccctgcccat cgaaggcatg
    14401 agctgcgcgt cctgcgccat cacggtgcag gaagcgctcg ccgcacagcc gggggtctcc
    14461 gccgccagcg tcaactacgc caccggccgc gcgtccgtcg actacgacga cgcgggcgcc
    14521 ggcatcgggc agatggtgcg ggccgtgcgg aacgcgggat acgactgcgg tctcgccaat
    14581 gtcaccatcg cgatcgtgga tctgcactat gcgccgagtg tcgcaccgct cgaaaaggcg
    14641 ctggcgcagg tccccggcgt ggtgaaggtc ctggcgaacc aggcgctgga gaccgcgtcg
    14701 gtcgaatacg ttccgggtgt cacctcggcg caggatctcg agggcgcggt ccgggcggcc
    14761 gggtttgcgg tggccgagcc gattccctcg gaggatccgg aggaacggga gcgcgtggcc
    14821 cgcgccttcg aagtcagggc actgacctgg aagttcgcgc tcgccgcggt ggtggccgtg
    14881 atcgcgatgc tcggcggcat gatcctcatg gcggaccggc ccctcgacac cttccaccgg
    14941 ttcgatctgc tgggccgggt cctgatgccc gtctccgagc ggctgcgcga cctggtgcag
    15001 gggaggtggc ccctcgattc cgactggatc cgccgcgtgt tggggctgct caccatcccc
    15061 gtcatggtct ggtcgggtgg acagttctac cgggccacgt ggagcggaat ccggcaccgg
    15121 accgcggaca tgaacaccct gatcgcggtg gggacgggcg cggccttcct ctactccgtc
    15181 gtggccacgg tgattcccgg cgtgttcctc caggccggac tccccgccga cgtctactac
    15241 gaggcggtct ccgcgatcat cgcgctgatc ctcttcggac ggctgctgga ggcgcgggcc
    15301 aagggtcaga cctccgcggc catccgcaaa ctggcgggat tgcgcccgaa aaccgcgcga
    15361 gtgcgccggg gtacggcgga gtccgacgtg ccgatcgagg cggtcgtcgt cgacgacttc
    15421 gtcatcatcc gccccgggga gcgggtgccg gtggatggga tcgtgaccga gggcgtttcg
    15481 tcggtggatg agtccatgct gaccggcgag ccgctgcccg tgacgaagaa ggtgggagac
    15541 gagatcgtcg gcgggacgat gaacacgacc ggcagcgtcg tcttccgtgc cacccgggtg
    15601 ggcaaggacg ccgcgctcgg ccagatcatc cggatggtcg aggaggcgca agggtcgaag
    15661 gccgccatcc agtcgctggc ggaccgcgtg gccggggtct tcgtccccgt ggtcatcgcc
    15721 atcgccatcg ccgcgttcgt cgtctggttc gacctcgggc cgtcgcccgc cgtgctcttc
    15781 gcgaccgtgg ccttcgtcac cgtcctcatc atcgcctgtc cctgtgccct cggcctcgcg
    15841 accccgaccg ccatcctggt cgggaccggc cgcgccgccg agctcggcat tctgatccgc
    15901 ggcgggcagg ccatcgagaa tctgtcgcga gtgaaagcga tcctgctcga caagaccgga
    15961 accatcacgg aaggccatcc ggtggtgacg cacatcctca ccgcgaagcg gagtgacggc
    16021 gcccccgtct ccccggcgga cgtcctgaag tgggccgcgt cggtggagca gcgctcggag
    16081 cacccgctgg cgctcgccat tctggatgcc gccaaggcca agggcgtcag catgctcgcg
    16141 gtggacaagt tcgcggcgat ggaagggcgc ggcgtccggg gcaccgtgga tcgccggatc
    16201 gtcgaagtga tctccctgcg tcacgcgcgc gagcgctcgc tcgacctggg cgggttggcg
    16261 aatgacgccg accggctggc cggggaaggg cgttcgccgg tgatcgtcgt cgtgaacaat
    16321 acggtctacg cgatcatcgc gatcgcggat cccatcaagt cgggtgcgcg agacgcggtg
    16381 gccgcgctcc tcgcgcgcgg cgtcgaagtg acgatggtct ccggggactc gaagcgcgcg
    16441 gccgaggcgg tggcgaagga agtcggcatc gacgacgtcg tggcggaggt gctgccttca
    16501 cagaaggctg atctggtacg gaagttccag cggaagggcc ggaaggtcgc gatggtcggg
    16561 gacgggatca acgacgcccc ggccctcgcg caggctgacg tcggaatcgc gatcggcacc
    16621 ggcgcggacg tcgcaatgga ggcatcggac gtcacgctga ttcgcgggga cttgcgggcg
    16681 gtggacacgg ccctggcgct gtcgcggaag acgctgtcga ccatccgccg caacctgttc
    16741 tgggccttca tctataatgt ggtcgggatt ccgatcgcgg ccggaatcct gtatcctgtg
    16801 accggcctgc tgttgtcccc cgtgatcgcc agcgcggcga tggcgtggtc gagcctctcg
    16861 gtggtgttga gcagtctgac cctgcgccgg ttcgaaccgg cggcctgacc ggagcgaatc
    16921 gatgcggtat ttccaccgga cccacttgtc tcctgatgaa gtgctggcgg cggctcagtc
    16981 gtacttcggc ggacgcctgg cgccctccga ggagaccaat cggcgacgct cgtttgcggg
    17041 agccttgggg aaggtgtccg tcgcggtgaa ggcggagggg gggcactaca ccttcatcga
    17101 cgtgaccacc gatcaggtcg gtgaagcgga gctcgacaag gtcgccaagc gctttctggc
    17161 gctggtgcac cagaaggccg atccctcgca ccaggtacga ggcgcctact aggccgtctc
    17221 ggctgcgacc gtggctcgat atccggcgcg cttcaccgcg tcgatgagca tttcattcgt
    17281 caccccgtcc cccgcttcca cttgagcggt gcccccgggc aggtccacct cggcgccgaa
    17341 tacgccgtct acgccgcgca gggccttctc gactttcgct tgacagtgcc cgcaagtcat
    17401 gccggacacc gatagagtcg tactcagcat gtcatcctcc gcttgatttg agcctccacc
    17461 gtccccaact ttcacgccca ccataattta cgacacgtgc ccgggtctac gtccgcatcc
    17521 aaagcgcccc caattccggc gggcttgagc cgagatcagc tactcgagat ctatcggttc
    17581 ttgcgtctta cccggacgct cgaggagcgt ctcaccgccc tctataggca aagcaaagtc
    17641 atcggcggtt tgttccggtc cctgggccag gagggggagt cggtagcgtc cgcctacgct
    17701 ctggagcggg gccggaaccg ggatttcctg tcccccctga ttcggaatct gggctccctt
    17761 ctggtcatgg ggggaaagcc cgtgcagatc ctgcggcagt acatggccaa ggatcaaagt
    17821 cccacccgcg gccgcgagct gaacgttcac ttcggggacc tggaaatggg attcctgggc
    17881 cagatctcgc acctgggaga catggtgccg gtgatggcgg gcatcacgct cacgttcaag
    17941 atgcggcggg agccccgcgt ggggctcgtc tatatcggag atggggcgac gtcgacgggc
    18001 gcgtttcacg agggactgaa tttcgcggcc gtccaacgct gtcccttggt ggtcatcggc
    18061 gaatacaacg gctacgccta ctcgaccacc ccggagcgtc agttcgcggt gaagaacctg
    18121 gcggacaagg cgaagggata cggaatcccg gcggtgaccg tcgacgggaa cgatgtgctc
    18181 gcggtgtacg aagcgacgaa gtacgccgtc gaccgggcac ggatggggaa gggtgtgcac
    18241 ttcgtcgaag tgaagacctt ccgcatgaag gggcacgccg agcacgacga ccagcattac
    18301 gtcgcgaagg ccgatctgga gcggtgggcg aagaacgatc cgatcgaccg ctattggaat
    18361 cagctgctca ccagcgggtg ggccgaggag caggatctga aggacatcga cagcgatgta
    18421 cgggacgaga tcgatcaggc gaccgatgcg tgcgtcaacg agccgttgcc gccggctgag
    18481 tccgccctga ccgccgtcta cgccgacccg ccggccgcgg agcggctttg gtaccggggg
    18541 ctgaatggct gaggtgacgt tcctcgaagg cctgcgtcag ggactgcgcg aagagatgga
    18601 gcgcgatgag cgcgtcttca tcctgggcga ggacgtcggc gcgtacggtg gagcgttcaa
    18661 ggtcaccgag gggttccaga agcaattcgg cgaatcccgg gtgatcgata cacccatttc
    18721 cgaggccgcc atcgtcggtg cggcggcggg cgccgcacat ttgggcatgc gcccggtggc
    18781 ggagatgcag ttcatcgact tcatctcctg cgcctacgac atgctgacga actacgtcgc
    18841 gacggcgcgg taccgggccg ggcttccgac gccgatggtc gtgcgcggcc cctgcggcgg
    18901 ctacgtccgc ggcgggccct ttcactcgca gaatcccgag gcggcgtttt tccatacgcc
    18961 gggcctcaag atcgtgtatc cgtccaccgt ccgggacgcg aaaggcctga tcaaggcggc
    19021 gatccgggat gacgatcccg tgctgtacct ggaacacaag tggctctacc gccgcatcaa
    19081 ggaccagatt ccggagggcg aggagatcat cacgcccatc ggatcggcac gcatcgcgag
    19141 ggaagggaag gacctctcga tcatcaccta cggagcgacc gtgtggaagt cgctcgaagc
    19201 ggccgaggtg ctcgagaagg atggcgtgtc cgtcgagatt ctcgacttgc gcacgctcct
    19261 cccgctcgac gatgatgcca ttctcaagac ggtgaagaag accaacaagg tgttgatcgt
    19321 gcacgaggat acccggaccg gaggcctggc cggcgagatc acggcgcgga tcaacgaaaa
    19381 agcgttcgag tggctggatg gaccgatcat gcgggtgacg gcgcacgacg taccgatccc
    19441 gtatgcgccg accctggaag acttcgtgct gccgcagacg gatgacatcg tcaaagcggc
    19501 gcgctggctc gctggttact aggagattcg agtatggctc gcgttgacgt tctgatgccc
    19561 cagatgggcg aatccatcgc cgagggcacc ctgtcgaagt ggctcaagaa ggtcggcgac
    19621 gacgtgaagc gcgacgagcc gctcttcgag atctcgaccg acaaggtcga cgccgagatc
    19681 ccggctccga cggccggggt gctggcggag atcaaggtgc agcagggcca gacggtcccg
    19741 gtgcagaccc tcgtggcggt gatcgagacg gagaaggggg ccgcggcctc cgcgcccgca
    19801 cccgctccag ccgctcccgc gccggccgcc aaggcggctc ccgcgccggc gcccgctcca
    19861 aaggctgccc cggcgcctgc cgccgcggct ccgatggccc ctccaagagc ccccgccccc
    19921 gcccccgctg cagtcccggc gaatggcgac gagacggccg aagaccgcct gcgccgccgg
    19981 tcgacgccgc tggtccggaa gatcgcggct gaacatcagg tggacatctc agggatctcg
    20041 ggaacggggt acgcgggccg ggtcaccaag caggacatcc tgggctacat cgagcgcggc
    20101 gttcccgccg gcgcgccgtc ggccccacgc cttccggcct ccgccccctc gggccccatc
    20161 gagcaccccg ccgtcgagcc gtggccgggc gaccgcgtcg agccctggtc caagatccgg
    20221 aagatcacgg ccgatcacat gatcatgtcg aagcgcgtgt ccgcgcacgt cgcctcgttc
    20281 ttcgaagtcg actttacccg cgtgtcgcag ctgcgcgcca aggcgaaggc gtcgtatcag
    20341 gagcggggcg tcaacctgac gttcctggcg ttcatcgcca aggcggtggc cgagaatctc
    20401 cggaagcatc ccgtggtcaa cgccgccgtc tccggggaca acacgatcta ccggcgcgac
    20461 atcaacctcg gcatcgccgt ggcgctcgat tggggcctca tcgtgccggt ggtgaagcac
    20521 gcggatgagc tgtcgctgat ggggatcgcg cgcgcgctca acgacctcgg cgagcgggcc
    20581 cggaccaaga ggctgacgcc cgacgagatt cagcgcggga ccttcacgat cacgaacccg
    20641 ggcatcttcg gggacctgat cggcatcccg atcatcaatc agccccaggt cgccatcctc
    20701 ggcgtcggag cgatcgagaa gaagccgagg gtcatcacgc agcccgatgg caccgacacc
    20761 atcgcgatcc gcaccatggg gatgctcagc atgtcctacg atcaccgcgt ggtggatggt
    20821 gccgacggcg acaagttcct ggcggacgtg aagaagatgc tccaggagtt cccggaagga
    20881 gcggtctgac catggcgaaa gtgctgaccg cggcgcgcgt ccgcgtgccc gcgtccaacg
    20941 agcgcgacta cctggccacc ctgcgcgatc tctgccagtt cgcggacgcc cggggccagc
    21001 ggatctgggt cttccgcaac gccaaggacc ctcagctgtt catcgaattc tccgaaagcc
    21061 cgacggagat gagtcaccgg gcgcaggcgt cccgcctgcc ggaagagatc aagctcgaga
    21121 agaagctgca gaacctggtg acctatgcgc ccgacgcctg ggagctctgg tcggaagttc
    21181 ccctcaccgc cgccgcggag gcgtgatgcc acaggcccgg ggcacgctga cggtcgacgg
    21241 tgaaacgtgg gagtacgcgc cgtccggcta cgacaccgtc tacaccaagg atcaattcgg
    21301 ggtggtcttc cagtgcggca ccggctccgc ccggcggcgc cggttcacgc ggttcacgcc
    21361 catgggaagc cggaatcctg aggcggccct gagcgagctc tcggatcgcc agctgctcga
    21421 gctgttccgc cagtcgcagc ccgcgtggac cgccccggag agcgcgtacg gcgcacgttg
    21481 atctgcattg ccactccacc gcgtcggatg gggagtgttc tccagaggag gtggcggcgc
    21541 gggcccacga ggcgaacctc gtcgccatcg ccctcaccga tcatgacacg ctcgacggcg
    21601 taggcgccgc cacggcggcc gccgcgccgc tcggcgtccg gatcgttccc ggctgcgaat
    21661 tcagcgtcaa ggcgccctgg ggtgagatgc acctcctcgg gtactttctc acgcccgggc
    21721 accaggcgct cgagtccttt ctggtcgaaa ccagagaggc ccggaagcgc cgcggacacg
    21781 agatggtcga acgcctcaag cgtgccggcg tgcccatcga tctcgccgac gtcgagtccg
    21841 aagtgaaaga cggggcgctc gggcgtccgc acgtggcgcg cgcgctgatc cggcgcgggg
    21901 cggccgacga cttcagcgac gccttcgcgc gtttcctggg acgcggcaaa ccgggctacg
    21961 tgcccaaacc tctcccgagc ctcgatacgg tcacgggatt gattcatcaa gtgggtgggg
    22021 tggcggtcgc cgcgcatctg ggcgaacacg gcaccgaacc gcagctgcgc cagttccagg
    22081 ccgcggggct cgacggtgtg gagattcgcc atccccggca ttcaccggcg gccgaggcca
    22141 ggctcgggcg cctggcggag aagctgggcc tggcggtgtc gggcggatcg gattggcatg
    22201 gagacggaga cgaaggcgcg tcgcatgcgc agctcggcgg gatggatgtg ccgctggagt
    22261 ggctggaaag actcgagaac cgtcgaggga agacgtccag gtaaggcgtc gagggaagac
    22321 gtatggcgag cttcgagaag aaggtggtgc tggtcactgg tgtcggacgc cccgggcaga
    22381 tcgggcatgc cgtcgccggg gcgttcggac gagcgggcgc gagcctcgtg atcgcggacc
    22441 gcgaacc
//
LOCUS       DASUBP010000291        11091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_37632, whole genome shotgun sequence.
ACCESSION   DASUBP010000291 DASUBP010000000
VERSION     DASUBP010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 11091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11091
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37632"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VFU75_11145"
     CDS             <1..198
                     /locus_tag="VFU75_11145"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HEU5467437.1"
                     /translation="KVDLLSDFKRDVSRRYDALLEEKFFSKRAYFLIDRAGVLRWTHT
                     EAELGHRREDAELLAQIAALV"
     gene            complement(205..1608)
                     /locus_tag="VFU75_11150"
     CDS             complement(205..1608)
                     /locus_tag="VFU75_11150"
                     /inference="COORDINATES: protein motif:HMM:NF012632.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467438.1"
                     /translation="MRPSPIVLLVILAACDSPFTPPPPTVGTAPTGTPKTLTLRPDSA
                     NLFADDTLQMLATVKDSAGQPLVMTVTWSVDDSNVVAIDPDGRAIAVDAGTTMVRVSV
                     AGLKDSTRIRVVRTVYQSIASGGTHSCAIGTNHRVYCWGADNDGQLGVKPQVLERPAP
                     TAIASGAVFQTVAAGQAHTCGSGTDFEMRCWGDNTAGQLGLGTTGGTSLPATATAPFD
                     FVLVTAGGDHTCALTATHVAECWGRNSSGELGVGDTASRNVPTLTADGRAFTAIAAGT
                     SHTCAETSTGAVLCWGVNAFGQLGDSSTAGQATPDSVRTSVAFDLLAAGGNHTCGLSS
                     GIAYCWGSNQRGESGTGATDSALVVPLAVSGPTFTALTLGSDFTCGLTISGSAYCWGA
                     NDNGQVGDSTQTDRSTPTLVRGGQYFTAIAAGASHVCAMTADGHVYCWGDGTSGQLGL
                     AVPVPLKTTPTLIPLPN"
     gene            1685..2752
                     /locus_tag="VFU75_11155"
     CDS             1685..2752
                     /locus_tag="VFU75_11155"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5467439.1"
                     /translation="MSAVALRGVTKTLDSASAPILHQVSLEVADGEFLTLLGPSGAGK
                     TTLLRCVAGLEQPTAGDVVIGDQVVTRSPPAARDVAMVFQAQALYPHLTVRGNLAFPL
                     EVRGASRAVIARKVLALAENLGLERMLDRRPGSLSGGEQQRVALARALVRDPKVLLLD
                     EPLGHLDPVSRADLAAAIARWHADRPVTTLHVTQDLTEAFTLARRVAVLDGGRIRQTG
                     TPRDVYLSPADTQVARMMGRPGMNVLGGTAHHLKAGTEVQCGPLLIPVPLREYEGQIT
                     IGIRPENVSIGPPGRGMGDATARLIEPSGPDTLLHLDTGEGVIVARIPGFLDVTPGAS
                     VGVTFDVTQIHFFDSSGKRLS"
     gene            2749..4518
                     /locus_tag="VFU75_11160"
     CDS             2749..4518
                     /locus_tag="VFU75_11160"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEU5467440.1"
                     /translation="MTRITLFRWAAGCAAVLSVASAYLATTGSRRLAERRYAERTAAS
                     AAAYLARVSTPKGVQPVPLDPAAFLRRVRVLATLPDWSPDVEVYFGTAPLVHPTEPSL
                     PLSTIAFLASQHSPEWFSGGAYAPIPGPTPGRVVGVVRVRPHLPADATAPWLLGLLGL
                     TALLGAVAATQLRRRRWARWLGAYALSAIGFGGVAASAAHAALRTSTIQWLQETAVLI
                     EDAGSRLQHTPTSALAETFKAMAADGTLTPDTSSDPVLERALAGTRPGSPLPIWLGGG
                     RWLSLQPPPEDWVVTARHAVAGTAALAGTILLIALSWGAGDRAHPGRIEETLSAWMFL
                     APAGAHLAALTIAPLGVLIWVAVHHWDLTTGGGSFVGVANLADVISHADTWGALGRTA
                     LFALHAPVVTAIALFLALMARRAPTPLVRVLLVVPPFASVAAAGLIWKNGLGAWGLFS
                     DPWSALWTLVGIAAVLQIGYQVPAFLAGLDRIPGVLWDAAVLDGAGAWTRFRRITEPL
                     LRPVTFSILLTGIVVAVQGFTLAFVTGPNAPDLAALHLYQVGWRQGRLDLAAALAVLL
                     AVGLAVFAALQVRIWGRGVIDEA"
     gene            4508..5416
                     /locus_tag="VFU75_11165"
     CDS             4508..5416
                     /locus_tag="VFU75_11165"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEU5467441.1"
                     /translation="MRRNWGTWGATLVAGLAGLVLAVPVLYEVSVSLMTPVESRALPP
                     AVVPLDPQFMNYLTGWSGLGAGGLLFTTALLAVLAAALQILTGAAAAFALARLRFPGR
                     RTMVRGVIVLAAIPSIVLLVPRWVMVDAWGWSDEFTGLAAPALVSAGAILFLYQVFRA
                     LPRGPEEAARLDGAGHWRVFSRVVVPQARPALAVVAGFAILAQWRGLLWPLVVSHRAG
                     PEVVEQGFARRLVEGASGMSVPELLAVGVTIAVPAMAIAACVALSRARAGTAARRTAL
                     TSGSRERQVPAEISALTPGPFEHQIL"
     gene            7410..7916
                     /locus_tag="VFU75_11170"
     CDS             7410..7916
                     /locus_tag="VFU75_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467442.1"
                     /translation="MASQSWLSLGLAATLGGGWQIEGGDIGLTQHTKFGPFRGVGETL
                     RLGSFVDEGAIIGGARGIVGGLALSARTGMLPIASMGSETNTSTLGADLTLEAAGTLG
                     SNSPFPQGSAWVSLSVLPGLRFGTNDGASYVIVVGPTVFWGRGHADVHTFLGIRFETP
                     LARGGTRS"
     gene            7929..9473
                     /locus_tag="VFU75_11175"
     CDS             7929..9473
                     /locus_tag="VFU75_11175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5467443.1"
                     /translation="MAAPIPLTQLPGLQVIAEISAMLGSGLDTDDTITSVLTVLRSRL
                     SIQVCRLWLRGPDGVSYATSPGSTELLPGERESNPVENWIKGGPTRTPRGLGTLVRLP
                     LLHDEVPFGALEAVVPTGPTEHMAHAVLSVVAGILAPLLAATELSQDLASEVAARTRE
                     LEAQRNFAARIIDSLPVGLYVIDADYTIRAWNRKRESGTQGVAREDAVGRLVFDVLDR
                     QPRDLLKREFDRVFETGEIQQVEMESQATRDARYYRITKIPMRIASDDHITHVITIGE
                     DVTEWREAQHRLAQTEKLAAIGQLAAGVMHEINNPLATILACAEALKLRDDGSSRGDE
                     YLEIIDKEVQRCRRIIDRLLDFSRPRTAEKRVVDINDVIEQTLFLLKHHERFKRMVVN
                     REFTEGLPTFPADPERLIQSFMALLLNAMDAMNQRGSLTVRSRMNPDRHDEIVVEFID
                     RGVGIKKEEISKIFEPFFTTKPQGRGTGLGLSICYGIIADHRGRIEVESQIGVGSTFK
                     VFLPIA"
     gene            9470..10138
                     /locus_tag="VFU75_11180"
     CDS             9470..10138
                     /locus_tag="VFU75_11180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5467444.1"
                     /translation="MKVLVVEDDKTVGQYVKRGLGEAGYQTDLVADGEEGLRLGSSGH
                     YDLIVLDLRLPKLTGPEVLRTLRDRGLSVPVLVLTAQDAVDHKVQALRAGADDYVTKP
                     FALEELLARVEALGRRPKEIASPSLKVADLVLDTGAREVKRAGKPIELTPKEYSVLEY
                     LVRHQGRVMSRTLITEYAWDYHFDPGTNIVDVVITRLRKKIDQGREPKLIHTVRGVGY
                     VVKT"
     gene            10139..>11091
                     /locus_tag="VFU75_11185"
     CDS             10139..>11091
                     /locus_tag="VFU75_11185"
                     /inference="COORDINATES: protein motif:HMM:NF012722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HEU5467445.1"
                     /translation="MQTIRGRLTAWYATAMALTLTAFASIVYVQGRSASYQELDQRIR
                     TEADQTNGILSEIYGSGGQLLQRGPNGGFVLVPQVAATLEPVPGFLLITASDSAGILF
                     ASADVRAGTFATLEQIHRLARTDTANHAVRTARIDPDGPTIRYVVRSAEGGPRFGTVL
                     AGADVAPTELGVQQLLTPIALVFPFSLLVAWLLGSWIARRALEPLDQIITEVEEISDG
                     RSLHRRLAQPMVRDEFGRLAETLNQMMGRLERSFVALRRFTADASHELKTPLTVLRAG
                     VERAVTTPNLPQETLVVLEETLQEVNRMTELVDALLTLARA"
BASE COUNT         1657 a         3748 c         3893 g         1793 t
ORIGIN      
        1 aaggtggatc tgctctcgga tttcaagcga gacgtgtccc gccgctacga tgcgctcctc
       61 gaggagaagt tcttttccaa gcgggcctac ttcctgatcg atcgcgcggg ggtcctccgc
      121 tggacgcaca ccgaagccga gttggggcac cgccgggagg acgccgagct cctggcgcag
      181 atcgcggccc tcgtttgacg ctcctcagtt ggggagggga atcagggtgg gcgtggtctt
      241 gaggggaacc gggacggcga gccccagctg gcccgaggtg ccgtcacccc agcaatagac
      301 gtggccgtcg gcggtcatcg cgcagacgtg ggaagcgccg gccgcgatgg cggtgaagta
      361 ttggccgcct cgcaccagcg tgggggttga ccgatcggtt tgcgtgctgt cgcccacctg
      421 gccgttgtcg tttgcacccc agcagtaggc cgatccgctg atcgtcaaac cgcaagtgaa
      481 atcactcccc agcgtcaggg ccgtgaacgt ggggcccgat accgccagcg gaacgacgag
      541 cgcgctgtcc gtcgcgccgg tgccggattc tcccctctgg ttggatcccc agcagtacgc
      601 gatcccggag ctcaggccgc aggtgtggtt accccctgcc gccaggaggt cgaacgcaac
      661 cgacgtgcgc accgaatccg gcgtcgcctg tccggccgtg gaggagtcac ccagctggcc
      721 gaatgcattc acgccccagc agaggacggc tccggtactg gtctcggcgc aagtgtgtga
      781 agtgccggcg gcgatggccg tgaaggcacg cccgtcggcc gtgagcgtcg ggacgttccg
      841 gctggcggtg tcgcccacgc ccagctcgcc cgagctgttg cgaccccagc actcggccac
      901 atgggtggcc gtcaacgcgc acgtgtgatc tccgccggcc gtcacgagga cgaaatcgaa
      961 tggggcggtc gccgtcgcgg ggagcgaagt tccacccgtc gtacccaatc ccagctgacc
     1021 ggccgtattg tcgccccagc agcgcatctc gaagtccgtc ccggagccgc acgtgtgtgc
     1081 ctgtcccgcg gcgacggttt gaaacaccgc gcctgacgcg atggcggttg gggcggggcg
     1141 ttccagcact tgcggtttga cgccgagctg tccgtcgttg tcggcgcccc agcaatacac
     1201 gcggtgatta gtgccgatgg cgcagctgtg ggtgccgccc gaggcgatgc tttggtagac
     1261 ggtacggacg acgcggatcc gggtggagtc cttgaggccg gcgacgctca cccggaccat
     1321 cgtggtcccg gcgtcgacgg cgatggcacg cccgtcgggg tcgatcgcga ccacgttcga
     1381 atcgtccacc gaccacgtga ccgtcatcac caacggctgg cccgcactgt ccttcacggt
     1441 ggccagcatc tgcagggtat cgtccgcgaa caggttcgcg gagtcaggac ggagggtcag
     1501 cgtcttcggt gtgccggtcg gtgccgtgcc gaccgtcggc gggggcggcg tgaacggcga
     1561 atcgcaggcc gcgaggatga ccaggaggac gatcggggac ggtcgcacag gtacagggat
     1621 ataccccgcc ggccttgggg gtgccaactg gcgcggggcc cgaggcggtc actagtttct
     1681 ttcaatgtcc gccgtcgccc tccgcggagt gaccaagacc ctcgactccg cttctgcccc
     1741 catcctgcat caggtgtcgc tcgaggtagc cgatggagag tttctgactc ttctgggacc
     1801 ctccggcgcc ggcaagacga cactgctccg ctgcgtcgcg gggctcgagc aaccgaccgc
     1861 cggcgacgtg gtcatcggcg atcaagtcgt gacgcggtcc cctccggcgg cgcgagacgt
     1921 cgcgatggtg ttccaggcac aggcgctcta cccccatctg acggtccggg ggaatctcgc
     1981 gttccccctg gaagtccgcg gggccagccg cgccgtcatt gcgcgaaaag tgctggcgct
     2041 ggccgagaat ctcggactcg agcggatgct ggaccggcgg cccgggagtc tgtccggcgg
     2101 tgagcagcaa cgcgtggccc tggcgcgggc gctggtgcgc gatccgaagg tcctcctgct
     2161 cgacgagccg ctggggcacc tcgacccggt gtcacgggcg gatctcgccg ccgccatcgc
     2221 tcggtggcac gcggatcgtc cggtgacgac gctgcacgtg acgcaggacc tgaccgaagc
     2281 cttcacgctg gcgcggcgcg tggccgtgct ggatggcggc cgaatccggc agaccggcac
     2341 gccccgcgac gtctatctgt ctcccgccga cacgcaggtg gcccggatga tggggcggcc
     2401 ggggatgaac gtgttgggtg ggacggccca ccacctcaag gcggggactg aagtccagtg
     2461 cgggccgttg ctgattcccg tcccactgcg ggaatacgag ggccagatca cgatcggcat
     2521 ccgtcccgaa aacgtctcga tcggcccccc cgggcgcggg atgggcgatg cgacggcgcg
     2581 actgatcgag ccatcgggac cggacaccct gctgcatctc gacaccggtg agggcgtgat
     2641 cgtggcccgg attccgggtt tcctggacgt gacgcccggg gcctcggtgg gagtgacgtt
     2701 cgacgtcacg cagatccact tcttcgactc gtccggcaag cggctctcat gacgcggatc
     2761 acgctgttcc gctgggcggc cgggtgcgcc gcggtgctgt ccgtcgcgtc ggcatacctc
     2821 gccacgacgg ggtcccgtcg tctggccgag cggcggtatg ccgagcggac ggcggcgagt
     2881 gcggccgcat acctggcccg ggtgtcgacg ccgaaaggcg tgcagcccgt gccactcgat
     2941 cccgcggcct tcctcaggag agtccgggtt ctcgccacgc tccccgactg gtccccggac
     3001 gtcgaggtgt acttcggcac ggcgccgctg gtgcatccca ccgagccctc cctgccgctc
     3061 tccaccatcg ccttcctggc aagccaacac tcaccggaat ggttttcggg gggggcttac
     3121 gcgccgatcc ccggacccac gcccgggcgg gtggtcggcg tggttcgcgt gcgcccgcac
     3181 ctcccggcgg atgcgaccgc accctggctg ctcgggctcc tggggctgac ggcactgctt
     3241 ggcgccgtcg ccgcgacgca gcttcggcgg cgccgctggg cccgctggct tggcgcctac
     3301 gcgctcagcg ccatcgggtt cggtggcgtc gccgcgagtg cggcccacgc ggccctgcgg
     3361 acgtccacca tccagtggct gcaggaaacg gcggtcctga tcgaggatgc ggggagccgg
     3421 ttgcagcaca cgcccacgtc ggcgctggcg gaaacgttca aggcgatggc cgccgacggg
     3481 acgctgactc cggacacgtc gtccgatccg gtactggagc gcgccctggc cggaacgcgt
     3541 ccgggatcgc cgctcccgat ctggttggga ggcgggcgtt ggctctcgct ccagccgcct
     3601 ccggaagact gggtggtgac ggcgcgtcac gccgttgcgg gtaccgccgc gctggcgggc
     3661 acgatcctgc tcatcgcact ctcctgggga gcgggcgatc gtgcccatcc ggggcggatc
     3721 gaggagacgc tttccgcgtg gatgttcctc gcccccgccg gcgcccatct cgcggcgctg
     3781 accatcgcgc cactcggcgt cctgatctgg gtggcggtgc atcactggga ccttaccacc
     3841 ggcgggggct cgttcgtcgg ggtcgcgaat ctcgccgacg tgatctcgca cgcggacacc
     3901 tggggcgcgc tcgggcgcac cgcactcttc gccttgcacg ctccggtggt gaccgccatt
     3961 gcgctctttc tcgcgctgat ggcccgtcga gcgccgaccc ctttggtccg ggtgctgctg
     4021 gtggtaccgc cgttcgcgtc cgtcgccgcg gccggactca tctggaaaaa cggcctcggg
     4081 gcgtggggac tcttctcgga tccgtggtcc gcgctctgga cgctggtcgg gattgcggcg
     4141 gtactgcaga tcggatatca ggtgccggcg ttcctcgcgg ggctcgaccg catcccgggc
     4201 gtgctctggg acgccgcggt gctcgacggc gccggggcgt ggacccggtt ccgccggatt
     4261 accgagccac tgctccggcc cgtgaccttc agcattctgc tgaccggaat cgtggtggcg
     4321 gtgcagggat tcacgctcgc gttcgtcacc ggtcccaacg cacccgatct tgccgccctg
     4381 cacctctatc aggtcggttg gcggcaggga cgactcgatc tcgccgcggc actcgcggtg
     4441 ctcctggccg tgggactcgc tgtattcgcg gcccttcagg tccggatctg gggccggggg
     4501 gtgatcgatg aggcgtaact ggggcacctg gggcgccact ctggtggccg gcctggccgg
     4561 tctggtgctc gcggtccccg tgttgtacga ggtatcggtg tccctgatga caccggtcga
     4621 atcgcgggca ttgcctccgg ccgtggtgcc cctcgatccg caattcatga actacctcac
     4681 ggggtggagt ggcttggggg ccggcggact gttgttcacg acggcactcc tcgcggtgct
     4741 cgcggccgcg ctccagatcc tgaccggtgc cgcggccgcg ttcgcgttag cgcggctgcg
     4801 gttcccggga cggcgaacga tggtacgtgg agtgatcgtg ctcgcggcga ttccgtcgat
     4861 cgtcctgctg gtgccgcggt gggtcatggt ggatgcgtgg ggatggtccg atgagttcac
     4921 cggcctcgcg gcaccggcac tcgtgtccgc gggggcgatt ctgttccttt atcaggtgtt
     4981 ccgggcgctc ccccgggggc ccgaagaggc ggcgcgactc gacggcgccg ggcactggcg
     5041 cgtgttctcc cgcgtcgtcg tcccgcaggc gcgccccgcc ctcgcggtcg tcgcgggctt
     5101 cgccattctc gcgcagtggc gggggttgct ctggccgttg gtggtgtcgc accgcgccgg
     5161 gcccgaggtc gtggagcagg ggttcgcgcg ccggctggtg gagggtgcgt ccggcatgag
     5221 cgtgccggag ttgttggcgg tcggggtcac catcgcggtc cccgccatgg cgatcgcggc
     5281 ttgtgtggcc ctatcgcgcg cccgggcggg caccgcggcg cgacggacgg cgctgacgag
     5341 cggctcgcgt gaacgacagg ttccagctga aatctccgcg ctcacccccg ggccattcga
     5401 gcaccagata ctctgagtcg agcacccgca ccgttccagg ccggtcaccc gtgagcgcat
     5461 ccgccgtcca tccccgggcg gcgatcgtga cgccgtaggg gcgcccgtcg agcttcaccg
     5521 cgcgcccact caacgtctgc gccttgccat cccagtgctc atcctgaaga tcgacgcacc
     5581 cctgtgccac gtggcgagtg gtcccgatga ccagcgggcg atcggcgcgc gagcgcaatc
     5641 ccagcaccag gcaatcgtgg gcctcgaggg tgacgtgcag aggatcagtg gccggcaagg
     5701 gcgagtcgtt ccagacgtcg taccccgtgt aggccccggg cgcgaggccg aggtcggcaa
     5761 ggcgaaccgc gcggtcgact ttctggtccg tccagttggc cagcatcacc gtggaccact
     5821 cgcctgagcg cgccacccag accgaagagc cgttcagggc atcgacgggg cgtccggcga
     5881 ccggcgccgg aggcacggcg cgccgcacga ggtcgacctg ggcgtcggga atgtccagaa
     5941 cgtcggcggt gaaggtcgtg gcggccccgg tcacggcggc gagactcagc tgggtccgcg
     6001 tttccacgag ggacagggga taccccagcg taatcgcgcc ggggtcgttc agccaccagg
     6061 cgcggtgata gtagctccgc agcccggcat tgttcgccat cacggtgacg gccttccagc
     6121 ccggcgccgg gtcggatccc acgcggacaa cgtccagcgt cccggtttgc ggaacgacag
     6181 acgcgctcca gatcgtggct gaacccgcgc cctcgtgaat cgcggccagc ccggcgcgga
     6241 ggctctcgag gttgggcagc tccgcgtcga ggccatggag gatcaacgcg tcgtatcccc
     6301 agtcctttac ggcgacccgt gcgccctcgc gcagctggtc gatcgacggg gcggcgccgg
     6361 tgaagggcgc ccacgatccc gccgccaaga gcccggcggc atgaatctga tcggtgcacc
     6421 aacgatgacc gcgcggggac ccgtcgcccg ggccgtacgc gacgtacctg gcgaagcgcg
     6481 ggtcgaacag atttgccgag cggcccagga tgtcgacaag gtcgtccccg cggccctgtg
     6541 agcccgagag ccagagcccg gcgggcggct cgagggtcgc caggaagtcc cgatctccgg
     6601 ccggaggcgc gcaggccgcg gtcagagcag ccacgccatc gccatgcggg tggtagcaga
     6661 tcgtgacggt ctgggcgtcg ccggccgtcg acacggggcg ggcgggggcc cagagactgt
     6721 ccagcgacag ttggtcgcca tttgtggacg cccgaaagac gcccgcgccg gtgggatcct
     6781 ggatgaggcc caacgcgcgc acgccgttcg acgtccggcg ggtcagcgcc agcgacccgt
     6841 tcgaccgtat ctcggagtgc ggttgcacca catcgggcgt ccgttcgtcg acctgggcga
     6901 gcaagtcgcc gggcccgggg agcgcgtggt cgacggggaa ggaggcccag gcgactccac
     6961 cgaccaccgg aaggctcgtg tccgggtaga ttcggatgct gatccggact cgcggcgcgg
     7021 ccgccgaggg ctccatcagg aaggtggcct cgacgaaaat cccggcaccg cggccgcgga
     7081 tcaccgtgga ctccccctgg acggggttgc cggcctgcag ggaaacgtcg tccaggtctc
     7141 cgagagtcag aatccggtca ctgaggtgga caccggcggg agtgaagggc agaaggggaa
     7201 cggcggggcc ttccccacgg taccacaacg accatcggtg ccggttgtcg aactcgaaag
     7261 acagacggcc gggcgtctct tcgaatgtcc atccggtgga tggacgcagc ggaaccagca
     7321 acgatccgga ggcagctccg gacaggagga cgaaggtgcg gcggttcatg gcggcaatgt
     7381 tctctgtcgg cgctcttttg tctacgccat tggcgtctca gtcgtggctc tcgctcggtc
     7441 tggcggccac cctgggcggc gggtggcaga tcgaaggtgg cgatatcggg ttgacgcagc
     7501 acacgaaatt cggtcccttc cgcggcgtcg gggaaaccct gcggctcgga tcgttcgtgg
     7561 acgagggtgc catcatcggg ggtgcccgcg ggatcgtcgg cggcctcgcc ctctccgcgc
     7621 gcaccggcat gctgccgatc gccagcatgg ggagtgagac caacaccagc acgctggggg
     7681 cggacctgac cctcgaggcg gccggcacac tgggctcgaa ctcgcccttt ccacagggga
     7741 gcgcctgggt ctccttgtcc gtgctccccg gacttcgctt cggcacgaac gacggtgcga
     7801 gctacgtcat cgtcgtgggc cccacggtct tctggggacg gggccacgcc gacgtccaca
     7861 catttctggg catccgcttc gagaccccgc tggcgagggg cggaacgcgg tcctagctta
     7921 gcggcctgat ggccgcaccc attcccctca cacagctccc cggcctccag gtcatcgccg
     7981 agatctcggc gatgctgggg agcgggctgg acaccgacga cacgatcacg tcggttctca
     8041 ccgtgctcag gtcccggctc agcatccagg tgtgccgcct gtggctgcgg gggccagacg
     8101 gtgtttcgta cgccacgagt cccgggtcca cggagctcct gcccggggag cgcgagagca
     8161 atccggtcga gaactggatc aagggtggcc ccacgcgtac cccgcggggg ctcggcacgc
     8221 tggtgcggct gccgctgctg cacgatgagg tgcccttcgg ggccctcgaa gccgtggtgc
     8281 ccacgggacc caccgagcac atggcgcacg cggtgctcag tgtggtggcc gggattctcg
     8341 cgcccttgct cgccgccacc gagctctcgc aggacctcgc ctccgaggtc gccgcacgga
     8401 cccgggagct cgaggcccag cgcaatttcg cggcgcgaat catcgattca ctcccggtgg
     8461 gactctacgt gatcgatgcg gactacacga tccgcgcctg gaaccggaag cgggaaagcg
     8521 gcacgcaggg tgtcgcgcgg gaggacgcgg tggggcgcct ggtcttcgat gtcctggacc
     8581 gccagccgcg cgacctcctg aagcgggagt tcgaccgggt gttcgaaacc ggggaaatcc
     8641 agcaggtcga aatggagtcg caggccacgc gggacgcacg gtattaccgg atcacgaaga
     8701 ttcccatgcg catcgcttcc gatgaccaca tcacccacgt catcaccatc ggtgaggacg
     8761 tgaccgagtg gcgggaagca cagcatcgtc tggcacagac cgagaagctc gcggccatcg
     8821 gccaactggc cgccggcgtg atgcacgaga tcaataatcc gctggccacg attctggcgt
     8881 gcgcggaagc gctcaagctc cgggacgacg gatcgtcgcg cggggacgag tatctcgaga
     8941 tcatcgacaa ggaagtgcag cgctgccgcc ggatcatcga ccggttgctc gacttctccc
     9001 gtccgcgaac cgccgaaaag cgcgtggtgg acatcaacga cgtcatcgag cagaccctgt
     9061 tcctcctcaa gcaccacgag cgtttcaagc ggatggtggt caaccgggag ttcaccgagg
     9121 gcttgccgac gtttccggcg gacccggaac gcctgatcca gagtttcatg gccctgttgc
     9181 tcaacgccat ggacgccatg aaccagcgcg ggtccttgac cgtccggtcc cgcatgaacc
     9241 cggaccgcca cgacgagatc gtggtcgaat tcatcgatcg cggcgtgggg atcaagaagg
     9301 aagagatctc caagatcttc gagcccttct tcacgaccaa gccccagggg cgaggcaccg
     9361 gcctcgggct ctcgatctgc tacggcatca tcgccgacca ccgcggccgg atcgaagtgg
     9421 aaagccagat cggcgtcggc tccaccttca aagtgttcct gcccatcgca tgaaagtcct
     9481 ggtcgtcgag gacgacaaga ccgtcggcca gtacgtgaag cgcggactgg gcgaggcggg
     9541 ataccagacc gatctcgtcg ccgacggaga agaggggctc cggctcgggt cgagtggcca
     9601 ctacgacctg atcgtgctcg atctgcggct gccgaagctc accgggcccg aagtgctccg
     9661 cacgctgcgc gaccgcgggt tgtcggtgcc ggtgctggta ctcaccgccc aggatgccgt
     9721 cgaccacaag gtccaggcgc tgcgggccgg tgccgacgac tacgtcacca agcccttcgc
     9781 gctcgaagag ctcctcgcgc gagtcgaagc gctgggccgg cgccccaagg agatcgcttc
     9841 gccttcgctc aaggtcgccg accttgtcct ggataccggc gcgcgggaag tgaagcgggc
     9901 cggaaaaccc atcgagctga cacccaagga gtactcggtc ctcgagtacc tggtgcggca
     9961 ccaggggcgg gtgatgtccc ggaccctcat caccgagtac gcgtgggatt accatttcga
    10021 tcccggcacc aacatcgtgg acgtcgtgat cacccgcctc cggaagaaga tcgatcaggg
    10081 ccgggagccc aagctgatcc ataccgtccg cggcgtcggc tacgtggtga agacgtaagt
    10141 gcagaccatc cggggccggc tcaccgcctg gtatgcgacg gcgatggcgc tgaccctcac
    10201 ggccttcgcc agcatcgtct acgtccaggg gcggagcgct tcgtaccagg agctggacca
    10261 gcgcatccgc accgaagccg atcagaccaa cggcattctc tccgagatct acggctccgg
    10321 cggtcagctc ctccagcgcg ggccgaacgg cgggttcgtg ctcgtaccgc aggtcgccgc
    10381 tacgctggaa ccggtgccgg gcttcctcct catcaccgcc tcggactccg ccggaattct
    10441 cttcgcctcg gccgacgtgc gcgccggcac cttcgccacc ctcgaacaga tccatcggct
    10501 cgcgcggacc gacaccgcca atcacgcggt gcgcacggcg cggatcgacc ccgatggtcc
    10561 cacgatccgg tatgtggtcc gctccgcgga aggagggccg aggttcggga ccgtgctggc
    10621 gggcgcggac gtcgcgccga ccgagctggg tgtgcaacag ctcctcacgc cgatcgcact
    10681 ggtatttccg ttcagcctgc tggtggcgtg gctgctcggg tcctggatcg cgcggcgcgc
    10741 gctcgagcct ctggaccaga tcatcacgga agtggaggag atctcggacg ggcggtcgct
    10801 gcaccggcgg ctggcgcaac cgatggtccg ggatgaattc ggccggctgg cggaaaccct
    10861 gaatcagatg atgggccgtc tcgagcggag cttcgtggcc ctgcgtcgtt tcaccgccga
    10921 cgcgagccat gagctgaaga cgcccctcac cgtgctccgg gccggggtcg agcgggcggt
    10981 gaccacgccc aaccttccgc aggaaacgct ggtcgtcctg gaagagacgc tgcaggaagt
    11041 gaaccggatg accgagttgg tggatgcgct gctcacgctc gcccgggccg a
//
LOCUS       DASUBP010000292        15070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_705909, whole genome shotgun sequence.
ACCESSION   DASUBP010000292 DASUBP010000000
VERSION     DASUBP010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 15070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15070
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_705909"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /gene="sucD"
                     /locus_tag="VFU75_11190"
     CDS             <1..810
                     /gene="sucD"
                     /locus_tag="VFU75_11190"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HEU5467446.1"
                     /translation="GSFHTRQMIAYGTQVVAGVTPGKGGQRFEEKVPVFNTVAEAVLA
                     TKPNVSVIYVPAAVAASAVFEAADAGIPLIVCITEGLPVLDMTKVMPYIRERGIRLIG
                     PNCPGLISPGQSKVGIIPGNICTPGKIGVVSRSGTLTYEIIHQLTGNGYGQSSCVGIG
                     GDPIIGTNFIDCLQAFEADPATDAIVMIGEIGGTDEQQAADFVKAKMRKPVVGFIAGQ
                     TAPPGRRMGHAGAIISGASGTAADKMKSLADAGISVMKRPADVVPLLKERM"
     gene            807..1715
                     /locus_tag="VFU75_11195"
     CDS             807..1715
                     /locus_tag="VFU75_11195"
                     /inference="COORDINATES: protein motif:HMM:NF012579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5467447.1"
                     /translation="MKLRDLLIPAHVAVPLQARTVREATHRLAEQLIQAGAVGDPEKL
                     LAVIDEAWPEEMVSVGEHAFLPHFRTDATPQLVAALGVAPKPIAWEKDPNRTARVVIL
                     VIAPPKEAASYLQLVGALARALSDHETVERLLGARTPADVVAVAGLDAVELTGDLTVR
                     DVMTEHVVTIDPERTLGEAAHLMLERDIRALPVVNEGGSLVGIVTHKELLKFLLPDYV
                     QRAKTGAFRAPTRNQILQGTTDPKQILVREVMARTVLCVSHDQRLSDVANLMNNKDVD
                     RFPVVREGTVVGFLTRADMIRRLLTP"
     gene            1789..2205
                     /gene="ndk"
                     /locus_tag="VFU75_11200"
     CDS             1789..2205
                     /gene="ndk"
                     /locus_tag="VFU75_11200"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEU5467448.1"
                     /translation="MLTLAIIKPDAVAAGNAGNVLAHLEKQGFKVRGAKMVRLSTPQA
                     EAFYAVHKDRPFFRSLVTFMTSGPCIPLALERDSAVTHLREVIGATDPAEAKPGTVRK
                     LFAQSKEKNAIHASDSPENATREVGFFFTEGELAGL"
     gene            2251..2760
                     /locus_tag="VFU75_11205"
     CDS             2251..2760
                     /locus_tag="VFU75_11205"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEU5467449.1"
                     /translation="MLQVDPKELARGPVDTPGEIAGDDPAFAGLGLQLVAPVRVAGRL
                     QEAGEGRFYWQGSFRTEVSGECRRCLAPVTVPIAGEVGALFSQETDAEDDPDCYAVPP
                     DARVIDVTPAVREELALTAPEFLLCREDCKGLCPRCGRDLNMGPCDCPPVTDPRWTGL
                     AGLKDKLSQ"
     gene            2779..2961
                     /gene="rpmF"
                     /locus_tag="VFU75_11210"
     CDS             2779..2961
                     /gene="rpmF"
                     /locus_tag="VFU75_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEU5467450.1"
                     /translation="MAVPKRRTSKRKKRARRTHYKAAMPTLQPCPKCGDLRQPHRVCP
                     TCGYYNGEQRVEVEDA"
     gene            2963..3976
                     /gene="plsX"
                     /locus_tag="VFU75_11215"
     CDS             2963..3976
                     /gene="plsX"
                     /locus_tag="VFU75_11215"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEU5467451.1"
                     /translation="MIRVALDAMGSDNAPQVEIEGAAQALRELPPTFRVQLVGRTADI
                     EAGLLEAGVSDRSRLEIVEAPEVVGMGEKPLQAIRGKPNSSIAVGLGLQKKNASDAFI
                     SAGNTGAVMAASTLLLRLHPGVERPAIGTVFPTAQQPVLLLDAGANVDCDPRELLGFA
                     QLGAVYARDVLGRTHPSVGLLNIGEEDEKGNAVVKEAHQLFKAATNFQYVGNVEGRDI
                     PAGECRGAPLDVVVCDGFVGNIVLKFYESAARVFVSLLKQEIPDILKRPEMGKVLKVL
                     DYSTYGGAPLLGVKGVSIICHGSSQPKAIRNAIRVAVQMVENDMNRHIDAELTQGTGN
                     AVA"
     gene            3973..4959
                     /locus_tag="VFU75_11220"
     CDS             3973..4959
                     /locus_tag="VFU75_11220"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HEU5467452.1"
                     /translation="MKRPFSQLSGTGSYLPARVMTNDEFSKILDTSDQWIRERTGIRE
                     RRISTKEESNACMGKKAAEIVLKENGLTALDIDAIVYATASPDRLLPSAACDVQALLG
                     AKNAAAFDIGAACSGFLYALNAADGLVASGQAERVLVIACEKLTSIMDWTDRSTAVLF
                     GDGAGATLMTKSDGKRGILSNYMKSDGTLAELLYRPGGGAAHPPSPEMIADHSYFIRM
                     AGREVYKAAVLSMADACDQALHRAGLSGKDLDLLIPHQANIRIIESTAKHAGIPMDKV
                     YVNVDRMGNTSAASVAIALDEAVRTGRLQPGMTAMFVAFGAGFTWASMVVKW"
     gene            4953..5888
                     /gene="fabD"
                     /locus_tag="VFU75_11225"
     CDS             4953..5888
                     /gene="fabD"
                     /locus_tag="VFU75_11225"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEU5467453.1"
                     /translation="MVSTIWLCPGQGAQKVGMGKDLAERFPIARQTFQAIDDALGVGL
                     SKIMFEGPEEQLTATSNTQPAILAHSAAVFAIVGSKVSTVAGAAGHSLGEYSAYVATG
                     ALTAPEAAKLVRRRGELMQLAGEARPGTMAAVLGLATADVEAACKEASQAGSIAVAAN
                     LNGPDQTVISGDPAAIERAGAICKTKGAKRVLPLKVSGAFHSPLMAPAADKLRLALDK
                     APFGDPKAPVIANATAEPVRDAARARRLLTDQLTAPVRWVACMEKAATLGEGLRFLEI
                     GPGAVLAGLLKRIVPGATVTSLGTADEVTKFMEGN"
     gene            5974..6708
                     /gene="fabG"
                     /locus_tag="VFU75_11230"
     CDS             5974..6708
                     /gene="fabG"
                     /locus_tag="VFU75_11230"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEU5467454.1"
                     /translation="MTGISGKVVLVTGGTRGIGYAIAKALVAAGAKVAICGRDGAKAA
                     AAAAGLGGTAKGYACDVADQTQVETLIESVEKDLGPIDVLVNNAGITKDNLLFRIGAE
                     DWDSVLDTNLKAAFLLTKLTARGMIKRRWGRIINISSVVGLTGNKGQANYAASKAGLI
                     GFTKSVAKELASRNVLVNAVAPGYIATEMTEAISDEMKQALQAAIPLGRLGQGEDIAH
                     AVLFLASDLASYITGQVLVVDGGMVM"
     gene            6758..6997
                     /locus_tag="VFU75_11235"
     CDS             6758..6997
                     /locus_tag="VFU75_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019948606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEU5467455.1"
                     /translation="MKQLEEKVKDIIVEELGVERDKLTNNASFMEDLGADSLDTVELV
                     MAFEKEFDIDIPDEEAEKLKTVGAAMQYLHEKMGK"
     gene            6998..8245
                     /gene="fabF"
                     /locus_tag="VFU75_11240"
     CDS             6998..8245
                     /gene="fabF"
                     /locus_tag="VFU75_11240"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEU5467456.1"
                     /translation="MRRRVAVTGLGLVTPLGNTVPETWDALVAGKSGAGPITRFDPTK
                     LPVKFAHEVKNWDPSLYLEKKEARRYDLFAQFAMGAAVQAVRDSCLEERWKELDLRRV
                     GVIIGTGTGGIATFEEQCQIYLEKGPSRVSPFFVPMFMGNVASAVVSMRYGAKGPNYC
                     TVSACATSAHALGDACRLIQHDEADIMISGGSEAAITPLAVASFANMKALSERNDDPT
                     TASRPFDKDRDGFVMGDGAACVILEEWEHARKRGATIYAEMVGYGMTADAYHITAPSP
                     DGEGAQRAMQLCMQDGGIRAEQVGYINAHGTSTPHGDAAETAAVKSVFGAQAKNLIFG
                     STKSMTGHLLGAAGALEFAACALAIRHGVVPPTINQFTPDPACDLNSAPNHAVKRQLE
                     VALSNSFGFGGHNVTLGLKRAES"
     gene            8242..9504
                     /gene="mqnE"
                     /locus_tag="VFU75_11245"
     CDS             8242..9504
                     /gene="mqnE"
                     /locus_tag="VFU75_11245"
                     /inference="COORDINATES: protein motif:HMM:TIGR03700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminofutalosine synthase MqnE"
                     /protein_id="HEU5467457.1"
                     /translation="MIPGGPRDARLDAIAAKVDRSERLDREDALTLFESHDLLTIGRL
                     ADQVNRRRNGSRVYFAANQHLNPTNVCILRNTCVFCSFARMPKEEGSYTRELDQVFAE
                     ADAARDNPTREFHIVGGLHPKLSLSYYEDLFRGLKERHPDVQIKALTAVEVAHLARIE
                     GISVRDVLVRMKAAGVTSLPGGGAEVFSPGARATIADKKLSGEEWLAVHRVAHGLGIP
                     SNCTMLYGHVETLADRVDHLLALRGLQDETKGFLTYIPLAYHPDHNELGEALGRTGTA
                     TSGFDDLKNIAVGRLVLDNIPHVKTHWPMVTPFISQIALGFGCDDLEGTVVFERVYHE
                     AGAGTPMWMSYDQIVGLIRGAGKEPVERNSLYETVRTFENWVPGQSSARPQGPQGPTF
                     ELAHRANASARPNRKPLPVLSPTGDGVE"
     gene            9501..10325
                     /locus_tag="VFU75_11250"
     CDS             9501..10325
                     /locus_tag="VFU75_11250"
                     /inference="COORDINATES: protein motif:HMM:NF014658.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone biosynthesis protein"
                     /protein_id="HEU5467458.1"
                     /translation="MRLGRIGYINCYPVYGAIDRGVVKVSAELVTGTPAELNDLLVAG
                     ELDVSVISAVEYARHSRDLVLLPDVAISSDGPVRSVMLFSRVPATALNDVPVLLSASS
                     RTSVGLLELLCRDVWKVRPRFAQVRAEAADLTALAGLPHQAVLVIGDPALLLGSRDAY
                     PHVYDLGTEWRRWTGLPFVFAVWAARRTGEPRAAIARVHAQILKSRDWGLANLDELAR
                     SASAATKVPEGECRSYFSGLDYALTDRHLAGLTLFFRKLVTHGLAPEGSLQFLHVA"
     gene            10327..11370
                     /gene="mqnC"
                     /locus_tag="VFU75_11255"
     CDS             10327..11370
                     /gene="mqnC"
                     /locus_tag="VFU75_11255"
                     /EC_number="1.21.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic dehypoxanthinyl futalosine synthase"
                     /protein_id="HEU5467459.1"
                     /translation="MVDRTSSEFRSFVDLYRDAPLLELGAQADATRWQLHPESVVTYI
                     IDRNINYTNVCVADCKFCAFYRRPKHPEGYLLSYEEIGRKIEELKALGGVQILMQGGH
                     NPYIPFEWYLDLLRYIKRNHPIHIHGFSPSEVDFFATRFGLSIEEVIRELVKAGLDSI
                     PGGGGEILVQSIRDQVAKKKAGADRWLEVMAIAHRMGLKTSVTMMYGLGETLEDRMEH
                     LFRVRDVQRETGGFTAFICWPLQPEHSQLEHLGKTDAVTYLRTQAIARLVLDNVPNIQ
                     ASWVTMGFKVGQVALRFGANDFGSLMMEENVVSAAGTTYRTTLGEMQRLIADAGYAPK
                     KRRQDYSLMPEAA"
     gene            11370..11768
                     /locus_tag="VFU75_11260"
     CDS             11370..11768
                     /locus_tag="VFU75_11260"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0034023 - 5-(carboxyamino)imidazole
                     ribonucleotide mutase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AIR carboxylase family protein"
                     /protein_id="HEU5467460.1"
                     /translation="MPEVLIVVGSESDKPRIEPAFDILTKAGVTFEFHASSAHRQPDQ
                     TGALIKGAKAAGAKVVIAGAGLSAALPGFAASMTDLPVIGVPFAVGPLNGLDALLATV
                     QVPPGVPVATVGIDNAKNAAHLALRILGKK"
     gene            11769..12236
                     /gene="ispF"
                     /locus_tag="VFU75_11265"
     CDS             11769..12236
                     /gene="ispF"
                     /locus_tag="VFU75_11265"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HEU5467461.1"
                     /translation="MRVGIGYDSHRFAPGRPLILGGVTIPHSLGLTGHSDADAVCHAL
                     TDAILGAAGLGDIGQMFPDTDPRWKGADSVKLLTQANLAVVEAGYHFGQADITVIAEA
                     PRMAPHIPGMQQRLADALVVSPSTISIKAKTNEGMGWIGRGEGIAAIAVATLA"
     gene            complement(12233..12733)
                     /locus_tag="VFU75_11270"
     CDS             complement(12233..12733)
                     /locus_tag="VFU75_11270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467462.1"
                     /translation="MRVPLGLMAAATLAAACHSATAPAPTVLGTWTFTMDSLSGMPRI
                     VPRTFTVTLQQADEGPVGMLPLLDDRADPGLNIVFVFDSLQVVTFSSRIDTTLATNNV
                     LVHGGDTLVAFTSIKASYDCGALAFVLPINAGRTEARGWVYLLSRSDFTTVCGDSAMV
                     TATKPR"
     gene            12820..13296
                     /locus_tag="VFU75_11275"
     CDS             12820..13296
                     /locus_tag="VFU75_11275"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HEU5467463.1"
                     /translation="MTGSAARDESGIRFPPPLIYALFFLTGYGLHRLEPVTLFAGQRW
                     PWLAGLVLIALGVVVAITAALTFRMAGTHVNPTKPATTVVAHGPFRFTRNPMYLSMAV
                     IYLGGVLMLDSVWPLVLFPVTIWVIRTRIIALEEAYLEAKFGPIYLDYKKKVRRWL"
     gene            13287..13811
                     /gene="msrA"
                     /locus_tag="VFU75_11280"
     CDS             13287..13811
                     /gene="msrA"
                     /locus_tag="VFU75_11280"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEU5467464.1"
                     /translation="MALATLAGGCFWCLEAVFQQLKGVSRVASGYAGGKRPNPTYEQV
                     CTGTTGHAEVVQVEFDPAVVSYRDLLDVFFTIHDPTTLNQQGADVGTQYRSAIFFHSP
                     EQQRDANDAIAEAQKTWDDPIVTEIVPLTQFYPAEEYHKDYYVRNPNQGYCRVVIAPK
                     VAKARQHFFEKLKA"
     gene            complement(13816..14178)
                     /locus_tag="VFU75_11285"
     CDS             complement(13816..14178)
                     /locus_tag="VFU75_11285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467465.1"
                     /translation="MALQGLIATHVTPETLTVTVTQVNATSYHVTMAAIGWGDVPVTF
                     DSGAAVLTSQDTTVFGFAEYPTAQNRTAVCNFVSFQGTKNSGLDTLTSASIALFNSDT
                     VPGGYCTPTLEGTVTIHK"
     gene            14394..>15070
                     /locus_tag="VFU75_11290"
     CDS             14394..>15070
                     /locus_tag="VFU75_11290"
                     /inference="COORDINATES: protein motif:HMM:NF015649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HEU5467466.1"
                     /translation="MLRLRLFGPLELSASDGRDLRPLLAQPKRVAVLACIAARPAGEF
                     VRRDSLLGLFWPELDQAAARRSLRQALHLLRTHLGPDALVSRGDEDLAVNPDQLSVDV
                     PDFLAAAGFRRGEALELYRGDFLAGFFLSGGSVEFERWLEETRSSLRDTAARLSSVLA
                     RESEGTGDREQAVAWARRALSYEPESESALRRLMETLDRAGDRAGAIRAYEGFTRRLA
                     QDLDQQPS"
BASE COUNT         2631 a         4969 c         5014 g         2456 t
ORIGIN      
        1 gggtcgttcc ataccaggca gatgatcgcc tacggcaccc aggtcgtggc cggggtcacc
       61 ccgggcaagg gcggccagcg gttcgaggag aaggtgccgg tgttcaatac cgtcgccgaa
      121 gccgttctcg ccaccaagcc caacgtcagc gtgatctacg taccggccgc cgtcgcggca
      181 tccgccgtat tcgaagcggc cgacgcgggg atcccgctga tcgtctgcat caccgaagga
      241 ctgcccgttc tggatatgac gaaggtcatg ccgtacatcc gcgagcgcgg gatccggctc
      301 atcggaccga actgccccgg actcatttcg ccgggacaga gcaaggtcgg catcatcccc
      361 gggaacattt gcacgcccgg caagatcggc gtcgtctccc ggtccggcac cctgacctac
      421 gagatcatcc accagctcac cggcaatggc tacggacagt cgtcctgtgt cggcatcggg
      481 ggggatccca tcatcggcac gaacttcatc gactgcctcc aggccttcga ggccgacccg
      541 gccaccgacg cgatcgtcat gatcggcgag atcggcggca ccgatgaaca gcaggcggcc
      601 gacttcgtga aggcgaagat gaggaagccg gtggtcgggt tcatcgccgg gcagacggcc
      661 ccgccgggac ggcggatggg ccacgcgggc gccatcatct ccggcgcgtc gggcactgcc
      721 gccgacaaga tgaagtccct cgcagacgca gggatctcgg tcatgaagcg ccccgccgat
      781 gtggtgcctc tgttgaagga acggatgtga agcttcggga tctcttgatt ccggcccacg
      841 tggcggtgcc gctgcaggcg cgcaccgtcc gtgaggccac ccaccgcctc gcggagcagc
      901 tcatccaggc gggggcagtg ggagatcccg agaagctgct cgcggtgatc gacgaggcgt
      961 ggcctgaaga gatggtgtcg gtgggagagc acgcgtttct cccgcatttc cggaccgacg
     1021 cgacccctca gctcgtggcc gcccttggcg tcgccccgaa gcccattgcg tgggagaaag
     1081 accccaaccg caccgcacgg gtggtgatcc tcgtgatcgc tccacccaag gaagcggcgt
     1141 cctatctgca gttggtggga gccttggcgc gcgcgctctc cgaccacgag acggtcgaga
     1201 ggttgctggg agcgcggacg ccggccgacg tggtggccgt agccgggctc gatgcggtgg
     1261 agttgaccgg cgatctcacc gtccgggacg tgatgacgga gcatgtcgtg accatcgatc
     1321 ccgagcggac gctgggagag gcggcgcacc tgatgctgga gcgggacatc cgcgcgctgc
     1381 ccgtggtgaa cgaagggggc tcattggtgg gcatcgtcac ccacaaggag ctgctcaagt
     1441 ttctgctccc ggactacgta caacgggcca agacgggtgc cttcagagcc cctacccgca
     1501 accagattct ccagggcacc accgatccga agcagatcct ggtccgcgag gtgatggccc
     1561 ggacggtcct gtgcgtctcg catgatcagc gcctctcgga cgtcgccaat ctcatgaaca
     1621 acaaggatgt cgaccgcttt cccgtggttc gggaaggcac ggtcgtcgga ttcctgaccc
     1681 gcgccgacat gattcggcgc ctgctaacgc cgtgaggcgt gctgacggct gacggctgac
     1741 agctgatggc catcagccgt cagcaaacaa cactctgaaa gggaagacgt gctcacgtta
     1801 gcgatcatca aacccgacgc cgtggctgct ggtaacgccg gaaacgtcct cgcccacctc
     1861 gaaaagcagg gcttcaaggt caggggcgcg aagatggtgc ggctctccac cccccaggcc
     1921 gaggccttct atgccgtcca caaggaccgg cccttcttcc gttcgctggt caccttcatg
     1981 acctccggcc cgtgcatccc cctggccttg gagcgggact cagccgtgac gcacctccgc
     2041 gaggtcattg gagccacaga tcccgctgaa gccaagccgg gtacggtccg gaagctcttc
     2101 gcccagtcga aggagaagaa cgcgattcac gcctccgatt ctcccgaaaa tgcgactcgc
     2161 gaggtgggat tcttcttcac cgagggcgaa ttggcggggc tgtaagagtt cgcttgacgc
     2221 aagtgccaca agggtttagc ttcgctgtcc atgctgcaag tcgaccccaa ggagctcgcc
     2281 cgcggtccag tcgacacccc gggagagatc gccggggacg accccgcgtt cgccgggctg
     2341 ggcctccagc tcgtggcacc ggtacgggtg gccgggcgtt tgcaggaggc aggagaaggg
     2401 cgattctact ggcaggggtc gttccggacg gaagtgtccg gggagtgccg gcggtgtctc
     2461 gccccggtaa ccgtgccgat agcgggcgag gtcggggcgc ttttttcaca agagactgac
     2521 gccgaagacg atcccgactg ttacgcggta ccaccagacg ccagagtcat cgatgtgacc
     2581 cccgcggtgc gtgaggagct ggcgctcacg gcacctgagt ttctgttgtg tcgagaagac
     2641 tgcaaggggc tctgcccccg ctgcggtcgc gacctgaaca tgggaccctg tgattgtccc
     2701 cccgtgaccg atccccgctg gacggggctg gccgggctga aggacaagct gtcccagtaa
     2761 cctgagcccg agaggcgtat ggctgtcccg aagcgcagaa cgtccaagcg caagaagcgc
     2821 gcgcggcgca cgcactacaa ggccgcgatg cccaccctcc agccgtgtcc caagtgcggg
     2881 gatctgcggc agccgcaccg ggtgtgtccg acctgcggct actacaacgg cgagcagcgg
     2941 gtcgaggtag aggacgcctg aggtgatccg ggtcgcgctg gacgccatgg ggagtgacaa
     3001 tgctccccag gtggaaatcg agggtgccgc gcaggccctg cgcgagctcc cgccgacgtt
     3061 ccgcgttcag ctggtaggcc gtaccgccga catcgaggcc ggattgctgg aggccggtgt
     3121 ctccgaccgg tcgcgtctcg aaatcgtgga ggcgcccgag gtcgtgggga tgggagaaaa
     3181 accgctgcag gccattcgcg ggaaacccaa ctcctcgatc gcggtgggcc tgggcctgca
     3241 gaagaagaac gcttccgatg ccttcatctc cgccggcaac accggcgcgg tgatggccgc
     3301 gagcacgttg ctgctccgcc tccacccagg cgtcgagcgg cccgccatcg gcaccgtctt
     3361 tccgaccgcc cagcagcccg tgctgctgct ggatgccggt gccaacgtgg actgcgaccc
     3421 gcgtgagctc ctcgggttcg cacagctcgg ggccgtgtac gcccgcgacg tgctggggcg
     3481 cacccatccc tccgtcggcc tcctcaacat cggagaggaa gacgagaagg ggaacgcggt
     3541 ggtgaaggag gcgcatcagc tcttcaaggc cgcgaccaac ttccagtacg tcggcaacgt
     3601 cgaaggccgg gacattccgg ccggcgaatg ccggggtgct ccgctggacg tggtggtctg
     3661 cgacggattc gtcgggaata tcgtcctgaa gttctacgaa tcggcggccc gggtcttcgt
     3721 ctcgctcctg aaacaggaga tcccggacat cctcaagcgg ccggagatgg ggaaagtcct
     3781 caaggtgctc gattactcca cgtacggcgg ggctccgctc ctcggcgtca agggcgtgtc
     3841 gatcatctgt cacggctcct ctcagcccaa ggccatccgg aacgcgatcc gcgtcgccgt
     3901 ccagatggtc gagaacgaca tgaaccgcca catcgacgcc gagctcaccc agggcaccgg
     3961 caacgccgtc gcatgaagcg ccccttctcg cagctctccg gtaccgggag ctacctgccc
     4021 gcgagggtca tgaccaatga cgagttctcg aagatcctcg acacgtcgga tcagtggatc
     4081 cgggagcgta cgggcatccg ggaacgccgg atctcgacca aggaagagtc caacgcctgc
     4141 atggggaaga aggcggcgga gatcgtcctg aaggaaaacg gtctcacggc cctcgatatc
     4201 gacgccatcg tctacgccac ggcgtctccc gaccgcctcc tcccgtccgc cgcctgcgac
     4261 gtccaggccc tcctcggggc caagaacgcc gcggccttcg acatcggcgc ggcgtgctcc
     4321 ggtttcctct acgcgttgaa cgccgccgat ggcctcgtcg cgtccggaca ggccgagcgg
     4381 gtattggtga tcgcgtgtga gaagctgacc agcatcatgg actggaccga ccggtccacc
     4441 gccgtgctgt ttggcgacgg cgccggcgcc acgctgatga cgaagagcga cggcaagcgc
     4501 ggcatcctgt cgaactacat gaagtcggat gggaccctgg ccgagctgct ctatcgcccc
     4561 ggtggcggcg cggcgcaccc ccccagcccc gagatgatcg ccgatcactc gtacttcatc
     4621 cggatggcgg ggcgcgaggt gtacaaggcc gcggtgctgt cgatggcgga cgcctgtgat
     4681 caggccctcc atcgcgccgg cctgtcgggg aaggacctcg atctcctcat cccgcaccag
     4741 gcgaacatcc gcatcatcga gtccacggcc aagcacgccg gcattccgat ggacaaggtc
     4801 tacgtgaacg tggaccgcat gggcaacacg tcggccgcct cggtcgcgat cgcgctggac
     4861 gaagccgtga ggaccggccg gctccagccc ggcatgaccg cgatgttcgt ggcgttcggc
     4921 gcgggcttca cctgggcgag catggttgtg aagtggtaag caccatctgg ctctgcccgg
     4981 gccagggcgc gcagaaggtg gggatgggaa aggatctcgc ggagcggttt ccgatcgcgc
     5041 ggcagacctt tcaggccatc gacgacgcgc tgggcgtcgg gctctcgaag atcatgttcg
     5101 agggacccga ggagcagctc acggccacca gcaataccca gcccgccatc ctcgcgcatt
     5161 ccgccgccgt cttcgccatc gtcggctcca aggtcagcac ggtcgccggc gccgcggggc
     5221 actccctcgg ggaatactcg gcctacgtcg ccaccggcgc gctcactgcg cccgaggccg
     5281 cgaagctggt ccggcgccgc ggcgagctga tgcagctggc gggcgaggcg cgccccggca
     5341 ccatggccgc ggtgctcgga ctggccacgg cggacgtgga agccgcgtgc aaggaagcgt
     5401 cgcaagccgg ttcgatcgcc gtcgccgcca atctcaatgg gccggatcaa acggtgatct
     5461 ccggtgatcc cgccgcgatc gaacgcgcgg gcgccatctg caagaccaag ggcgccaagc
     5521 gcgtgctgcc gctcaaagtg tcgggcgcgt ttcattcccc cctgatggca ccggccgccg
     5581 acaagttgcg cctcgcgctc gacaaggcgc ccttcgggga tccgaaggct cccgtcatcg
     5641 ccaacgccac ggccgagccg gttcgcgacg ccgcgcgcgc tcggcgcctg ctcaccgatc
     5701 agctgacggc gccggtgcgc tgggtcgcct gcatggagaa agcggccacg ctgggggagg
     5761 gactgcggtt cctcgaaatc gggccaggcg cggtgctcgc ggggctcctc aagcggatcg
     5821 tgcccggcgc gacggtgacg agcctgggaa ccgcggacga agtgacgaag ttcatggagg
     5881 gcaattgaat tcccagactg aagtctgggc ccggcagggc gaggccctta agggcagcgt
     5941 ggtgtcgggc ccacgcttga gcgtgggaac gacatgaccg ggatcagtgg caaggtcgtg
     6001 ctcgtcaccg gcggcacccg cggcatcggc tacgcgatcg ccaaagccct ggtcgccgcg
     6061 ggcgcgaagg tcgcgatctg cggccgggac ggggccaagg cggcagcggc ggcggccgga
     6121 ctgggcggca ccgcgaaggg atatgcctgc gacgtcgccg accagaccca ggtcgagacg
     6181 ctgatcgaga gcgtcgagaa ggacctcggt cccattgacg tgctcgtgaa caacgccggg
     6241 attacgaagg acaacttgct gttccggatc ggcgccgagg attgggacag cgtgctggac
     6301 acgaacctca aggccgcgtt tctgctcacg aagctcacgg cccggggcat gatcaaacgc
     6361 cgctgggggc gcatcatcaa tatctccagc gtggtgggac tcaccggcaa caagggccag
     6421 gcgaactacg cggcctcgaa ggccggattg atcggcttca ccaagagcgt ggcgaaggag
     6481 ctggcatcgc gtaacgttct cgtgaatgcc gtcgctccgg gatacatcgc gaccgagatg
     6541 acggaagcca tctcggacga gatgaagcag gccctccagg cggccattcc gctgggaagg
     6601 ctggggcagg gcgaggatat cgcacacgcc gtgctctttc tcgcttccga tctggcgagc
     6661 tacattacag gacaggtgtt ggtcgtcgac ggcgggatgg taatgtgatc ccgcgagagt
     6721 ccacccctct gcactaggat caaccatggc catggacatg aagcagctgg aggagaaggt
     6781 caaggacatc attgttgagg agctcggggt cgagcgcgac aagctcacca acaatgccag
     6841 cttcatggag gacctgggcg ccgacagctt ggacacggtc gagctggtga tggccttcga
     6901 aaaggaattc gacatcgaca ttcccgacga ggaagccgaa aagctgaaga ccgtcggggc
     6961 ggcgatgcag tatctccacg agaagatggg gaagtaggtg cggcgacgcg tggcggtcac
     7021 gggcctcggg ctcgtgaccc cgctgggcaa caccgtgccg gagacgtggg acgcgctggt
     7081 ggccggaaag tccggcgccg gccccatcac gcgcttcgat cccacgaagt tgccggtcaa
     7141 gttcgcccac gaggtgaaga actgggaccc gtcactctac ctcgagaaga aagaggcgcg
     7201 gcggtatgac ctcttcgccc agttcgcgat gggcgccgcc gtgcaggccg tgcgggattc
     7261 ctgcctcgaa gagcgctgga aggagctgga cctccgccgc gtcggtgtga tcatcggcac
     7321 gggcacgggc ggtatcgcga ccttcgagga gcagtgccag atatatctgg agaagggtcc
     7381 gtcccgggtg tcccccttct tcgttccgat gttcatgggg aacgtcgcgt ccgccgtggt
     7441 gtccatgcgg tacggcgcga aggggccgaa ctactgcacc gtctccgcgt gcgcgacctc
     7501 cgcgcatgcc ctgggggacg cgtgccggct catccagcac gacgaagcgg atatcatgat
     7561 ctcgggcggc tcggaggcgg ccatcacgcc gctcgccgtc gccagcttcg cgaacatgaa
     7621 ggcgttgtcc gagcgcaatg acgatcccac caccgccagc cgcccgttcg acaaggaccg
     7681 cgacgggttc gtgatgggcg acggcgccgc ctgcgtcatc ctcgaggagt gggagcacgc
     7741 caggaaacgg ggcgcgacga tctacgccga gatggtcggc tacggcatga cggccgacgc
     7801 gtaccacatc acggccccct cacccgacgg cgagggcgcc cagcgggcga tgcagctgtg
     7861 catgcaggat ggcggcatca gggcggagca agtcggttac atcaacgccc acggcacgtc
     7921 gactccgcac ggcgacgccg cggaaaccgc ggccgtcaag agcgtgttcg gtgcacaagc
     7981 gaagaatctg atcttcgggt ccaccaagtc gatgaccggc cacctcctcg gcgcggcggg
     8041 tgcgctcgag ttcgccgcct gcgccctcgc gatcaggcac ggtgtggtgc cgccgaccat
     8101 caaccagttc acccccgatc cggcctgtga cctgaattcc gcgccgaatc acgcggtcaa
     8161 gcgccagttg gaagtcgcgt tgtccaacag cttcggcttt ggggggcaca acgtgacgtt
     8221 ggggctgaag cgagccgagt cgtgatcccc ggaggaccac gggacgcgcg gctcgacgca
     8281 atcgccgcga aggtggaccg gagcgagcgc ctcgaccgcg aagacgccct cacgctgttt
     8341 gaatcgcacg acctcctcac catcggccgg ctcgccgacc aggtgaaccg gcgacggaac
     8401 gggtcgcgcg tctacttcgc cgccaatcag cacctcaacc ccaccaacgt ctgcatcctc
     8461 cgcaacacct gcgtcttctg ctcgttcgcc cggatgccga aggaggaggg gtcgtacacc
     8521 cgcgagctcg atcaggtgtt cgcggaagct gacgccgccc gggacaaccc gacccgcgag
     8581 ttccacatcg tcggtggtct gcaccccaag ctctcgctgt cgtactacga agacctgttc
     8641 cggggcctga aagagcggca tccggacgtc cagatcaagg cgctgaccgc ggtcgaggtc
     8701 gcgcatctgg cgaggatcga gggcatctcc gtgcgcgacg tgctggtgcg gatgaaagcc
     8761 gccggggtta ccagtctccc gggtggaggg gcggaggtgt tctcacccgg cgcgcgcgcg
     8821 accatcgcgg acaagaagct gtcgggagag gagtggctcg cggtccaccg cgtcgcccac
     8881 ggcctgggga tcccgtccaa ctgcaccatg ctctacggcc acgtcgagac gctcgccgac
     8941 cgggtggatc atctcctggc gctgcgaggc ctccaggacg agacgaaggg attcctgacg
     9001 tacatcccgc tcgcctatca ccccgaccac aacgagctgg gtgaggcgct gggccgcacc
     9061 ggcaccgcca cgtccggctt cgatgatctc aagaacatcg ccgtcgggcg cctcgtgctg
     9121 gacaacatcc cgcacgtgaa gacccactgg cccatggtca ccccgttcat ctcccagatc
     9181 gcgctcggct tcggctgcga cgatctggag ggtaccgtgg tgttcgagcg cgtctaccac
     9241 gaggccggtg ccgggacgcc catgtggatg agctacgatc agatcgtggg actcatccgc
     9301 ggggcgggga aggagccggt ggagcgcaac tccctgtacg agacggtccg cacgttcgag
     9361 aactgggtgc cgggtcagtc gtccgcgcgc ccccagggcc cccagggccc gacgttcgag
     9421 ctcgcgcacc gggccaatgc cagcgcccgg ccgaatcgca agccgttgcc ggtgttgtcg
     9481 cccacgggag atggagtgga atgagactcg gccggatcgg ctacatcaat tgttatccgg
     9541 tatacggggc catcgaccgc ggtgtcgtca aagtctccgc ggagctggtc accggcaccc
     9601 ccgccgagct gaatgatctg ctcgtagcag gggagctgga cgtatcggtg atcagcgcgg
     9661 tggaatacgc ccggcactcg cgggacctcg tcctcctgcc ggacgtggcg atctcatcgg
     9721 atgggccggt gcggagcgtg atgctcttca gccgcgtccc cgccaccgcc ctgaatgacg
     9781 tccccgtgct gctttccgcc tcgtcccgca cctccgtcgg gttgctcgag ctgctctgcc
     9841 gcgacgtctg gaaggtgcgg ccacgattcg cacaggtacg ggcggaagcg gccgatctca
     9901 ccgcgctcgc gggattgccg catcaggcgg tgctcgtgat cggcgacccg gcgctgctgc
     9961 tggggtcgcg ggatgcgtac ccgcacgtct acgatctcgg cacggaatgg cgccgctgga
    10021 cgggcttgcc gttcgtgttc gcggtgtggg cggcgcggcg gaccggggag ccccgggcgg
    10081 ccatcgctcg cgtccacgcc cagatcctca agtcgcgcga ctggggactc gccaacctgg
    10141 acgaactggc ccgctcggcg agcgccgcca ccaaggtccc ggaaggagag tgccgcagtt
    10201 acttctcggg cctcgattac gcgctgacgg accggcacct ggccggtctg accctctttt
    10261 tccggaaact ggtgacccac ggcctcgcgc ccgaaggctc actccagttt ctgcacgtgg
    10321 cctgacatgg tcgatcgcac gtcatccgaa ttccgctcgt tcgtggacct ctatcgtgat
    10381 gcgccgctcc tcgagctggg ggcccaggcc gacgccaccc ggtggcagct ccatcccgag
    10441 agcgtggtca cgtacatcat cgatcggaac atcaactaca cgaacgtctg tgtcgccgac
    10501 tgcaagttct gcgcgttcta ccgccgtccg aaacaccccg aggggtatct gctgtcctac
    10561 gaggagatcg gacggaagat cgaagagctg aaggcgctgg gcggcgtaca gatcctgatg
    10621 cagggcggac acaaccccta catcccgttc gaatggtatt tggacctcct gcggtacatc
    10681 aagcggaacc atccgattca cattcacgga ttctcgccat cggaggtcga tttcttcgcg
    10741 acccgcttcg ggctgagcat cgaagaagtc attcgtgagc tcgtgaaggc gggcctcgac
    10801 tccattccgg gcggtggcgg ggagatcctg gtgcagagca tccgggacca ggtcgccaag
    10861 aagaaggctg gcgccgaccg ctggctcgag gtcatggcca tcgcccaccg gatggggctc
    10921 aagaccagcg tgacgatgat gtacggcctg ggcgagacgc tcgaggaccg catggagcac
    10981 ctcttccggg tccgcgacgt ccagcgtgag accggcgggt tcacggcctt catctgctgg
    11041 ccgctccagc ccgagcacag tcagctggag cacctcggga agaccgacgc cgtgacgtat
    11101 ctccgcacgc aggcgatcgc ccgcctcgtg ctggacaacg tgccgaacat ccaggcgtcg
    11161 tgggtgacga tggggttcaa ggtgggacag gtggccctac ggttcggcgc caacgacttc
    11221 ggttcgctga tgatggaaga gaacgtcgtg tcggccgcgg gcacgaccta tcgcaccacc
    11281 ctcggagaaa tgcagcggct catcgccgac gccgggtacg cgcccaagaa gcgtcggcag
    11341 gactactcgc tgatgccgga ggccgcgtaa tgccggaagt actgatcgtc gtggggtccg
    11401 agagtgacaa gccgcgcatc gagccggcgt tcgacattct caccaaggcc ggcgtgactt
    11461 tcgagtttca cgcctcatcc gcccaccgcc aacccgacca gaccggcgcc ctcatcaagg
    11521 gcgccaaggc cgcgggggcc aaagtcgtca tcgccggagc gggcctgtcg gcggcactcc
    11581 ccgggttcgc cgcctcgatg accgacctgc cggtgatcgg cgtcccgttc gcggtaggcc
    11641 cgctgaacgg cctcgacgct ttgctcgcga ccgtgcaggt gcctccgggg gtgccggtcg
    11701 ccacggtcgg gatcgacaac gccaagaacg cggctcacct ggcactccgg attctcggca
    11761 agaaatagat gcgcgtcggg atcgggtacg attcccaccg cttcgccccc ggccggccgc
    11821 tgatcctcgg cggcgtcacc attccgcact ccctggggct caccggacac tcggacgccg
    11881 acgccgtctg ccacgccttg accgacgcga tcctgggcgc cgccgggctt ggggacatcg
    11941 gccagatgtt tccggacacc gatccgcgat ggaagggcgc cgactccgtc aaactcctga
    12001 cccaggcgaa cctggcggta gtggaggccg ggtatcactt cgggcaggcg gatatcacgg
    12061 tgatcgccga ggcaccccgg atggcgccgc acatccccgg catgcagcag cgcctggccg
    12121 atgccctggt ggtcagcccc tccaccatct cgatcaaggc caagaccaac gagggcatgg
    12181 ggtggatcgg ccgcggggag ggcatcgccg cgatcgccgt cgccaccctc gcctagcgcg
    12241 gtttggtcgc ggtcaccatc gccgaatccc cgcacacggt ggtgaagtcg cttcgcgaca
    12301 gcagatagac ccagccccgg gcctcggtcc ggccggcgtt gatgggcagc acgaaggcca
    12361 gggccccgca atcgtagctc gctttgatcg acgtaaacgc caccagggtg tccccaccgt
    12421 gcaccaggac gttattggtc gccagggtgg tatcgatccg gctcgagaac gtgacgacct
    12481 ggaggctgtc gaacacgaag acgatattca gaccgggatc agcccggtcg tcgagcaggg
    12541 gcagcatccc gacggggcct tcatcggctt gctgcagggt caccgtaaag gttcggggca
    12601 cgatccgggg catcccggac agagagtcca tggtaaacgt ccaagtcccg agcacggtcg
    12661 gcgcgggagc ggtggcgctg tgacaggcgg cggccaacgt cgcggcggcc atgaggccaa
    12721 gcggaactcg catgggagct ttctcgttga gtggaaggcg catcctaggg tgcgaccgtc
    12781 accgcgccgt cacccttgtc gcattcggga ggaggtatca tgaccggctc cgctgcccgt
    12841 gacgaatcgg ggattcgttt cccgcccccg ctcatctacg ccttgttctt cctgaccggt
    12901 tacgggttgc accggctcga gccggtcacc ctgttcgccg gacagcggtg gccctggctc
    12961 gccggcctgg tcttgatcgc tctgggggtg gtcgtcgcga tcacggcggc actcacgttc
    13021 cgcatggccg gcacccatgt gaatccgacc aagcccgcca ccacggtcgt ggctcacggg
    13081 cccttccggt tcacacggaa tcccatgtac ctcagcatgg cggtgatcta tctgggtggg
    13141 gtgttgatgc tcgactcggt gtggcccctg gtactgtttc cggtgacgat ctgggtcatc
    13201 cgaacccgga tcatcgcgct cgaagaggcg tacctggagg cgaagttcgg cccgatatac
    13261 ctcgactaca agaagaaggt gcggcggtgg ctctagcgac tttggcaggc ggatgcttct
    13321 ggtgcctcga ggcggtcttt cagcagctga agggtgtgtc ccgggtggcg tcgggctatg
    13381 cggggggaaa acgtcccaac cccacctacg aacaggtgtg caccggaacg actggccacg
    13441 ccgaggtggt gcaggtcgaa ttcgacccgg cggtggtcag ctatcgggac ctgttggacg
    13501 tgttcttcac catccacgat cccacgacgc tgaaccagca gggtgccgac gtgggtaccc
    13561 agtaccgctc ggccattttc ttccactctc ccgagcagca gcgcgacgcg aatgacgcga
    13621 tcgccgaggc gcaaaagacg tgggacgacc ccatcgtcac ggagatcgtc ccactcaccc
    13681 agttctatcc ggccgaggag taccacaagg actactacgt ccggaatccg aaccagggct
    13741 actgccgggt ggtcatcgcc ccgaaggtgg cgaaggcccg gcagcacttc ttcgagaagc
    13801 tcaaggcctg accccctatt tgtgaatcgt cacggttcct tcaagcgtcg gcgtgcagta
    13861 cccccctggg acagtgtccg aattgaagag tgctatcgat gcgctcgtca gcgtatccag
    13921 ccctgagttc ttcgtcccct ggaagctgac gaaattgcag acagccgtcc ggttctgagc
    13981 cgtggggtat tcggcgaacc cgaacacggt cgtatcctgg gacgtcagga cagcagctcc
    14041 gctgtcgaag gtcaccggaa cgtcgcccca tccgatcgcc gccatggtca catgatagga
    14101 tgtcgcattc acttgcgtca ccgtaacggt gagcgtttcg ggtgtgacgt gcgtggcgat
    14161 gagcccttgg agcgccacgt gccaggtccc ggcaaggctc ggcttggggc tcgtagagga
    14221 ggaacaggct gccagaaatc caaccagtac tccgcaggat aaccacgatt gccgcatgaa
    14281 gccctcctgg acccgaggtg gatgggggac cgtcaaaccc gcactttatc ccccatgcgt
    14341 cggccaaccg ttacttgtcg ggtaacggcc cgcgccctac cttcgtctcg tccatgctcc
    14401 gccttcggtt gttcggtccc ctcgagcttt cggcctccga tggccgggat ctccggcccc
    14461 tcctcgcaca accgaagcga gtcgccgtgc tcgcctgcat cgcggcacgc ccagcgggcg
    14521 aatttgtgcg ccgcgactcc ctcctcggcc tcttctggcc cgagctcgat caggcagccg
    14581 cccgacgctc cctccgccag gcccttcatc tattacggac ccacctggga cctgatgcac
    14641 tcgtctcccg gggggacgaa gacctggcgg taaatcccga tcagctgagc gtcgatgtgc
    14701 cggacttcct cgccgccgcc ggattccggc gaggggaggc gctcgagctg taccggggag
    14761 acttcctggc cggcttcttc ttgagcgggg ggtcggtcga gttcgaacgg tggctcgagg
    14821 agacgcggag ctcactccgg gacacggcgg cccgcctctc ttcggtgctg gcgcgggagt
    14881 ccgaaggcac cggcgaccgg gaacaggcgg tcgcctgggc ccggcgggcg ctctcctacg
    14941 agcccgaaag tgagtcggcg ctccggcgac tcatggaaac gctggaccgt gcgggggacc
    15001 gagcgggcgc gattcgggcc tacgaagggt ttacccggcg gttggcgcag gatctcgacc
    15061 aacaaccctc
//
LOCUS       DASUBP010000293         3249 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_502072, whole genome shotgun sequence.
ACCESSION   DASUBP010000293 DASUBP010000000
VERSION     DASUBP010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3249)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3249)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3249
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_502072"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..559
                     /locus_tag="VFU75_11295"
     CDS             <1..559
                     /locus_tag="VFU75_11295"
                     /inference="COORDINATES: protein motif:HMM:NF025067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HEU5467467.1"
                     /translation="GRWFRILARTLLALLLVIILSALNRMRLYVAAYGLSEIRLYATA
                     AMAYVTFLASWLGARLIRTRRRRFAPGALVAGYVALTGLHVTNPDALIVRVNMSRATT
                     RAFDARYAASLSADAIPALVAGLPKLDPVTRCQAEQSLRRRWLRIDESKHDWRSDNWA
                     SLSELKLARHWASIPFVRCPTPEAK"
     gene            556..1203
                     /locus_tag="VFU75_11300"
     CDS             556..1203
                     /locus_tag="VFU75_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467468.1"
                     /translation="MITDGLTPLGVLLLLGLVGGALALGVGIVAALLMDRGVWVKRFV
                     ITGVAGVALYVVLLFGLSLTSRTRILALTQGKHICEIDCHSEYSVVAVRTDKHFGGRT
                     AQGTFYIVTMKVLFDSTTTSARRGRAPLEIGPRTVYVLDAQGHEYPPQPDGASDSLRH
                     PIVPGQSYTTDLVFDLPAAISNPMLFVGATAGAPDRILIGSENSVLHKKTLFALS"
     gene            complement(1200..1688)
                     /locus_tag="VFU75_11305"
     CDS             complement(1200..1688)
                     /locus_tag="VFU75_11305"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEU5467469.1"
                     /translation="MSEGIAIRLATEADARVVARHRVAMFRDMGTLPAEQGPPLERAA
                     EAYFRRAIPQGSYVGWLATPEGRPGEIIGGAGIQLRESIPRLRLDRAAVDPGPQGLIV
                     NVYTEQAWRRRGVAQLLMRAILRFAKERGVDNVVLHASAEGRSLYEKLGFKQTNEMRY
                     ET"
     gene            complement(1705..3195)
                     /locus_tag="VFU75_11310"
     CDS             complement(1705..3195)
                     /locus_tag="VFU75_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU5467470.1"
                     /translation="MPAGLFATQPLERLRAAAGQEGGGLRRTLGPLNLTTLGIGAIVG
                     TGIFVFTGMAAAQYAGPAVPISMVIAAIACGFAGLCYAEMASAVPVAGSAYTYSYATL
                     GELIAWIIGWDLILEYAMGAATVAVGWSSTLGSMLGLFGITPPAAVMNPPFIACTAAQ
                     VAQGMATGCTQVGWHLTGAVFNLPAALSILAITAILIIGIRESASFNTTIVIIKVGVL
                     ALFVGFGFLYVKSANWHPFIPENNGQFGGWSGILRGAGLMFFAYIGFDSVSTAAQEAR
                     NPQKDMPVSILGSLVICTVLYVLVSTVLTGLAPYQDLNVPSPVIMVVDSIKEFGWFRY
                     AITFGAILGLGSTMLVMLLGQSRVLYAMSRDGLIPEWAGRVHPKFRTPYISTALTGIA
                     VALFAGLFPIQILGKLVNIGTLLAFVLVCMGVWVLRKTRPDLERPFKTPLVPLVPILG
                     ALACLGLMLTLGVDTWLRLVVWLAIGLVIYFRYGRHHSALRREGTP"
BASE COUNT          558 a         1101 c         1059 g          531 t
ORIGIN      
        1 tgggcggtgg ttccggatac tcgcccgcac gctgctcgcg ctgctcctcg tcatcatcct
       61 ctcggccctg aaccggatgc gcctctacgt cgccgcgtac ggcctctccg agatcaggct
      121 ctacgccacc gcggccatgg cgtacgtgac cttcctggcc tcctggctcg gcgcgcggct
      181 catccgaacc cgaagacggc gtttcgctcc gggagccctg gtagccggct atgtcgctct
      241 caccggactg cacgtgacca accctgacgc gctcatcgtc cgcgtgaaca tgagccgggc
      301 cacgacgcga gccttcgatg cccggtacgc cgcgtctctc tccgccgacg cgattcccgc
      361 tctcgtcgcc ggactgccga agctcgatcc cgtcacccga tgccaggccg agcagagtct
      421 gcgtcgccgc tggctccgga tcgatgaatc gaagcacgac tggcgcagtg acaattgggc
      481 cagcctgagc gaactcaagc ttgcccggca ctgggcgagc atccccttcg tccgctgtcc
      541 caccccggag gccaaatgat cacggatgga ctcactcccc tcggcgttct cctgctcctc
      601 ggtttggtcg gcggggcgct cgcgctcggc gtcggcatcg tcgccgcact gctcatggac
      661 cgcggcgtct gggtgaagcg gttcgtgatc acgggcgtag cgggggtcgc gctgtacgtc
      721 gtgctgctgt tcggcctgtc gctcaccagc cgcacccgga ttctcgcact cacccagggc
      781 aagcatatct gcgagatcga ctgccattcg gagtactctg ttgtcgcggt tcgcacggac
      841 aagcacttcg gcgggcgcac cgcccagggg accttctaca tcgtgaccat gaaggttctc
      901 ttcgattcga ctacgacttc ggcgcggcgt ggcagagcgc ccctcgaaat cgggccacgg
      961 actgtgtacg tgctcgacgc gcagggccac gagtatccgc ctcagcccga cggagcctcg
     1021 gactcgctgc gccatcccat cgtgcccgga cagtcgtata cgactgacct ggtcttcgac
     1081 ctcccggccg cgatttcgaa tccgatgctg ttcgtgggtg ccacggcagg agctccggac
     1141 cgcatcctga tcggatcgga gaacagcgtg ctgcacaaga agactctgtt cgcgttgagc
     1201 taggtctcgt agcgcatctc gttcgtttgc ttgaagccga gcttctcgta gagcgaccgg
     1261 ccctcggctg acgcgtggag cacgacgttg tcgacgccgc gctccttggc gaaccgcaaa
     1321 atcgcacgca tcaggagctg ggcgaccccg cgccgtcgcc acgcctgctc ggtgtagacg
     1381 ttcacgatga gcccctgcgg cccgggatct acggcggcgc ggtcgagccg gaggcggggg
     1441 atcgactccc gcagctggat gccggctccg ccgatgatct ccccgggtcg gccctcgggg
     1501 gtggccagcc agccgacgta actcccctgg ggaatcgccc ggcggaagta ggcctccgcc
     1561 gcccgttcga gcgggggacc ctgctccgcc ggcagagtcc ccatgtcccg gaacatggcg
     1621 acgcgatgtc gggcgactac ccgcgcgtcc gcctcggtgg ccagtctgat cgcgatcccc
     1681 tcggacatct cccgctccgg tgcatcacgg tgttccctcc cggcggaggg cgctatggtg
     1741 acggccgtag cggaagtata tcacgagccc gatcgccaac cacacgacca ggcgcagcca
     1801 ggtgtcgacg cccagcgtga gcatgaggcc gaggcaggcc aacgcgccga ggatggggac
     1861 cagcgggacc aggggcgtct tgaagggccg ctcgagatcg ggacgggttt tccggagtac
     1921 ccacacgccc atgcacacga gcacgaaggc caggagagtg ccgatgttca cgagcttgcc
     1981 gagaatctgg atcgggaaga ggccggcgaa gagcgcgacg gcaatgccgg tgagcgctgt
     2041 agagatgtag ggcgtcctga acttcgggtg gacccggccc gcccattcgg ggatcaggcc
     2101 gtcgcgcgac atggcgtaca gcacgcgcga ctgccccaaa agcatcacca gcatcgtcga
     2161 gccgagtccc agaatggcac cgaaggtgat ggcgtaccgg aaccagccga actccttgat
     2221 gctgtccacc accatgatga ccggagacgg gacgttcagg tcctgatagg gcgccagacc
     2281 cgtgagcacc gtcgaaacca gcacgtacag gaccgtgcag atgacgagtg agccgagaat
     2341 gctgacgggc atgtccttct gcgggttccg cgcctcttgc gccgccgtgc tcacggaatc
     2401 gaagccgata taggcgaaga acatcagccc cgccccgcgc aggatgcccg accagccgcc
     2461 gaactgtccg ttgttctcag ggatgaacgg atgccagttg gcgctcttga cgtagaggaa
     2521 cccgaacccg acgaacaggg ccagcacccc cactttgatg atgacgatcg tggtgttgaa
     2581 gctcgcggat tcccggatgc cgatgatgag aatcgcggtg atggcgagga tcgagagggc
     2641 cgccgggagg ttgaacaccg cgcccgtcag gtgccacccc acctgcgtac acccggtcgc
     2701 catcccctgc gccacctgcg ccgcagtgca ggcgatgaac ggtgggttca tgacggcggc
     2761 gggcggcgtg atgccgaaca gcccgagcat cgagccgagg gtcgacgacc agccgacggc
     2821 gaccgtggcg gcgcccatgg cgtattcgag gatcaaatcc cagccgatga tccacgcgat
     2881 gagctcaccc agggtcgcgt aggagtacgt gtaggcgctc ccggccaccg gtaccgcgct
     2941 cgccatctcc gcgtaacaga gccccgcgaa gccgcaggcg atggcggcga tcaccatcga
     3001 gatcgggacg gccggcccgg cgtactgcgc ggccgccatc cccgtgaaga cgaagattcc
     3061 cgtgccgacg atcgccccga tgccgagggt ggtgaggttg agcggtccga gggtgcggcg
     3121 gagcccaccc ccctcctgac cggcggcggc gcggagccgc tcgaggggct gggtggcgaa
     3181 caaaccggcg ggcatcgggc ctcctgtttt ggaatgcata attggataca attatgctta
     3241 acatacgtc
//
LOCUS       DASUBP010000294         2454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1006513, whole genome shotgun sequence.
ACCESSION   DASUBP010000294 DASUBP010000000
VERSION     DASUBP010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2454
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1006513"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VFU75_11315"
     CDS             <1..365
                     /locus_tag="VFU75_11315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467471.1"
                     /translation="SARGANDLPDDPHQLGVAILRQLPVGSYVPDPLRAQLRMLGAVA
                     GGTYVLAPASLFFKVDTTKSREPGAGSRRMGTAELTMVLVDVRLGQVSWRSVARGNGE
                     DPWSAVNAALKTLTPGLP"
     gene            362..1375
                     /locus_tag="VFU75_11320"
     CDS             362..1375
                     /locus_tag="VFU75_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HEU5467472.1"
                     /translation="MTREATLIPGDGIGPEITAATLEVLNALGMKFDWDEQYGGMSAV
                     EKAGTPLPNATLDSIRRTRLALKGPLTTPVGGGYRSVNVALRQEFDLYANVRPTKTIV
                     PGGRYPHVDLVIIRENTEGMYVGLDHVFRVADDPKAVAQAMSVMTRDGCMRICRYAFE
                     YAKKNGRKKVTIVHKANILKATSALFLESGRRVAKEYEGVIASDDKIVDNTAMQLVIN
                     PEQFDVIVTTNLFGDILSDEASGLVGGLGLAPGANIGTTASIFEAVHGSAPDIAGKGI
                     ANPSAQMLAAAMLLDHVGEQKAATKLRHAIETAIVKDNVRTKDLGGSASTSEFARAVA
                     RRV"
     gene            1637..>2454
                     /locus_tag="VFU75_11325"
     CDS             1637..>2454
                     /locus_tag="VFU75_11325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467473.1"
                     /translation="MKRTGIRGQGSGVSLLLFLLATPLVAQTSLSIYSDGRVVVRKTI
                     PQKLQRGTNIFSLDLPGLDPATLFSPDSNVAVTSAVLRPATDMESALRASIGRTLSFV
                     QGKGDTIQATVLSVNPPQTKLSNGMVMFSFPNGTPLFPAEASLIRSKAEAVVTLDASR
                     ARDATDLAYLSQGVTWEAVYQAVVNGSGSALVTGAATINSSTIKVDSAAVQLVAGSVN
                     VDAAVAMARRSPMAAGVALREEAKSGYATEETVGETHVYELPGLVSFDLGAPLTT"
BASE COUNT          440 a          778 c          822 g          414 t
ORIGIN      
        1 gcagtgcgcg cggcgcgaac gacctccccg atgatccgca tcagcttggg gtcgcgattt
       61 tgcgccagct cccggtgggg tcgtacgtac cggatcccct ccgggcgcag ctccgaatgc
      121 tgggggcggt ggcgggtggg acgtacgtcc tcgcgccggc ctccttgttc ttcaaagtgg
      181 atacaacgaa aagccgagag ccgggagccg ggagccgtcg catgggaact gcggagctga
      241 cgatggtgct ggtggatgtg cgtctcgggc aagtgtcctg gcggtcggtt gcgcgaggga
      301 atggggaaga tccctggagc gccgtcaacg ccgcgctgaa gacactcacc cccggcctgc
      361 cgtgacgcgc gaggctacgc tgattccggg tgacggcatc ggtcccgaaa tcaccgctgc
      421 caccctcgaa gtcctcaacg cgctggggat gaagttcgac tgggacgagc aatacggcgg
      481 gatgtccgcc gtcgagaagg ccggcacgcc gctccccaac gcgaccctcg attccatccg
      541 ccgcacccgc ctggccctca agggcccgct cacgacaccc gtcggcggtg gataccggtc
      601 ggtgaacgtc gcgctccggc aggaattcga cctctacgcg aacgtccgcc cgacgaagac
      661 catcgtgccc gggggccgtt acccccacgt cgacctcgtc atcatccgtg agaacaccga
      721 gggcatgtac gtcgggctcg atcacgtctt ccgggtggcc gacgatccga aggccgtggc
      781 gcaggccatg tcggtcatga cgcgcgacgg ctgcatgcgg atctgccggt atgcgttcga
      841 gtacgccaaa aagaatggcc ggaagaaggt caccatcgtc cacaaggcga acatcctcaa
      901 agccacctcc gcgctcttcc tcgagagcgg ccggcgcgtg gccaaggagt acgagggagt
      961 gatcgcgtcg gacgacaaga tcgtggacaa cacggcgatg cagttggtga tcaatccgga
     1021 gcagttcgac gtcatcgtga ccacgaatct gttcggcgac attttgtccg atgaagcgtc
     1081 gggactggtc ggcggtctgg ggctcgcgcc cggcgctaac atcggcacca ccgcgtcgat
     1141 cttcgaagcg gtccacggct cggcgcccga tatcgcgggg aaggggatcg cgaatccgtc
     1201 agcgcagatg ctcgccgcgg ccatgctgct cgaccacgtg ggtgagcaga aagcggccac
     1261 caaactgcgg cacgcgatcg agaccgcaat cgtgaaggac aacgtccgca ccaaggatct
     1321 ggggggcagt gcttcaacat ccgagtttgc ccgggctgtc gcccggcggg tatagccacc
     1381 tctgtaaagg ctgcgaccgc atgatcgccg gcctcgccgt cggcgagctg tgccaggagt
     1441 gcaccatccg ggtacggcgg cgggcgggcc ggatcgcccg atgggccgcc atcctaacta
     1501 caactccctt ggccatttac gtgacactta ccctgcctcc gcaagtcacg ccccgaatga
     1561 cctacgttgg ggttgtgctc gcctggtacg ctttgacgtt tctcatagtc aaacgaaccg
     1621 cctgggagtg gctgaaatga aaaggacagg gatcaggggt caggggtcag gggtcagcct
     1681 actgctgttc ctgctggcca ctccgctggt ggcccaaacg tcattgtcca tctacagcga
     1741 tgggcgggtg gtggtacgga agaccatccc gcagaaactc cagcgaggga cgaacatttt
     1801 ctcgctcgat ctcccgggcc tcgatccggc gacgctcttt tcgccggatt cgaacgtcgc
     1861 cgtgacgagt gcggtgctgc ggccggcgac cgatatggaa tcggcactcc gggcgtcgat
     1921 cgggcggacg ctttcattcg tgcaggggaa gggggacaca attcaggcga cggtcctgag
     1981 cgtgaacccg ccacagacca agctctccaa cggcatggtg atgttctcgt tccccaacgg
     2041 gacgccgctc tttcccgccg aggcgagctt gatccgctcg aaggccgagg cggtggtcac
     2101 cctcgacgcg agccgggcgc gggatgcgac ggacctcgcg tacttgagtc agggtgtgac
     2161 ctgggaggcc gtgtaccagg ccgtggtcaa cggctccggg tcggcgctcg tgacgggcgc
     2221 ggcgacgatc aattcttcca ccatcaaggt ggacagcgcg gcggtccagc tggtggccgg
     2281 ctcggtgaac gttgatgccg cggtcgcaat ggcacggcgg tcaccgatgg ctgctggtgt
     2341 ggccctccgc gaggaagcca agagcgggta cgccacggaa gaaacggtgg gcgaaacgca
     2401 cgtgtatgag cttccgggac tcgtgtcctt cgacctcggc gcgcccctca ccac
//
LOCUS       DASUBP010000295        15113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_365905, whole genome shotgun sequence.
ACCESSION   DASUBP010000295 DASUBP010000000
VERSION     DASUBP010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 15113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15113
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365905"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..894
                     /locus_tag="VFU75_11330"
     CDS             <1..894
                     /locus_tag="VFU75_11330"
                     /inference="COORDINATES: protein motif:HMM:NF040138.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HEU5467474.1"
                     /translation="LPPPPPMATSLQFTAQPQSAQTGGSLGTFTVAAVDSAGTVVTDF
                     NGTVTLAIGTNPGGGTLSGTASVALGNGVATFSGLSIDKAGNGYTLTASTSGLKSAAS
                     SPFNVTTPPPTTGSLTVTTATTGSNLPTSGYTISLDGGSGQAIGINGSLTLNNLTAGS
                     HTVTISGLASNCTVGGGTSQAVTVPAGGSTSASFAITCVAPPPTNHPPVVNAGGNQSA
                     VEAVLYQLNASFTDVDGDGPWTYDVNWGDGTSSSGTLSAQGAIGPSHTYVLPSDYTIT
                     VTVTDAHGASGADSKTLHVTL"
     gene            complement(884..1369)
                     /locus_tag="VFU75_11335"
     CDS             complement(884..1369)
                     /locus_tag="VFU75_11335"
                     /inference="COORDINATES: protein motif:HMM:NF017015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HEU5467475.1"
                     /translation="MPIDPTATLLSAYSASARINQYLVEHLHPSLWRAKVPGPKVRTI
                     AALVAHVHNCGLIYLRRAAPKVPVPPELDRFSVTQKQAVKVLGAKRKAVLRIVTQCLK
                     GNGRVDGFSGGNAAMFLAYYMSHDAHHRGQIVTLARLLGHPVSTKTMSGMWQWSVRSK
                     E"
     gene            complement(1761..4643)
                     /locus_tag="VFU75_11340"
     CDS             complement(1761..4643)
                     /locus_tag="VFU75_11340"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HEU5467476.1"
                     /translation="MDITPLENQRKAIEAPLGPVLVVAGPGAGKTFCLISRIEYLISN
                     QGIAPDRICVVTFTNKAAEEIAVRLSKVLGDRAEELTRGTLHGLCLHLLRERADQIGL
                     ARGFGVADEPYQLAILARLKVYAKERTSLLNRFSRRRMQDFKLSAKDEQAFRDYSDYL
                     IRRNLVDFDDIIVRTDTLLRNHPVVADEISARWDYLLVDEFQDLSPMQYAILKRLAPH
                     RNFFAVGDEDQSIFSWTGADPKMLDSFRRDYGVTPIVLDRNCRSSRQIFDVARRLVEE
                     NPQLFHKQLSADRDAAYPVRALGFGDEESEASWLISDIKEDMAANGGDWGDYALLYRR
                     HQVGDLLESHLLRAGVPCRLARGRSLNEDDVVGPVIRALRIVRDPTDQAAILSLAEAM
                     LPADLYLEVEAGTRAGDVLAAVRSLAQKRPRKDPDAKKLWQFVFQCENFEALSRSERT
                     LGGLVDELLSERLTPYRNVLEERHDDLTDPADLPEAVRLAEGLKRVMAGTGEAVVEPM
                     NGLGLALRAMLVAAGLRNVGTPDANRRAGPDDVLIGPDSAGSSGLALTVFKALQLAHA
                     AESTESFDRFVTFDTETTGTDPDTAEIVDLAAVRVVRGQVVEKFSRLVRPEHGVPSSA
                     TKVHGYSDADLADAPSFAEVWPDFKEFVGNDLMVAHNGLQYDVPILRRLAGEEQTSDM
                     VFFDTLPLARSLFKESAKLTDLSARFNVDSGDAHHALDDALALASVFQHLTRLRLLRA
                     RKAVLVEILGHLAVALTFEPAARAAGEGKTLFDIGRFRALGRFSDALEYYEVDHERTR
                     LGPPVDEVVKGLGGAALRARLQGDYDPELAQPRAVARLRALMGEDAALPIAEGITKLL
                     ERVALSSRDGVSTDPQRVTLLTLHSTKGLEFSRVYIVGAEDFQIPGYRTMRDQVEDEI
                     QEGRRLLYVGMTRAKDRLILTRADQRFGMPAGGNQFLDEMGLVPET"
     gene            4724..5404
                     /locus_tag="VFU75_11345"
     CDS             4724..5404
                     /locus_tag="VFU75_11345"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEU5467477.1"
                     /translation="MAVRQVLLASLVPLAMLTSRPPHHVVSPEAWRDSVRAFAEAHLQ
                     HTAWGPAHARRDYEVTVWLAREEKVPVDTDALYAAAYLHDMGGLPPYALPGVDHGDRS
                     IQLVDTLLNAWGFPMEKSPLVKEIIDHHQYYRPADTLRIAMLFRDADILDFMGAIDVA
                     RIVSLTTRERLAPDLGHAVQVIKKQMTDMPALLQSATARKEGQRRVAEMRAFLDALAL
                     ESDSLSNL"
     gene            complement(5405..7903)
                     /locus_tag="VFU75_11350"
     CDS             complement(5405..7903)
                     /locus_tag="VFU75_11350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HEU5467478.1"
                     /translation="MTLRWEPLVQDLRYAVRGLRSKPAFTVAVTLTLALGIGANTAMF
                     SVVDRLLFRPPALLRDAARTHRVYLAETYRGKEYNSGSTQYARYLDLTSFTHSFERTA
                     EVTARHMAIGIGTEAREMQVGAVSASFFGFFDAPPAVGRYFTTAEDTFPQGAAVAVLA
                     YGYWQTRFGGRSDVLGQTIQIGATPYTIIGVAPRGFVGLWADEPPAAFVPIATYGAEI
                     GANLRLRGENWWSTYHWRWAWMIAQRKPGVTIAAADADLTAADLKSLGVEYAQSPSPD
                     RTPLSLSKPHGLVASILSERGPNESGEAKVATWLGGVALIVLLIACANVANLLLARAL
                     QRKREIAIRLALGVSRLRLASQLLSESLLLALLGGVAGLLVAQFGGAILRAYLLPKTA
                     QATVITDPRTLIMVGAATLVAGLLTGLAPVFQTRRADLTGDLKAGAREGAIHRSRTRV
                     ALLVLQGALSVVLLVGAGLFVRSLSHIRHLPLGFDPDSVSVVDLQMRGVKLDSAASVA
                     LRRRLLAEARTIPGVTHVSRQITMPFWSSWNQDIHVAGIDSTDRLGSFYLNAVTPDYF
                     ATMGTRLLRGRGVTESDVAGSPRAMVVSQAMANVLWPGKEALGECVRVGDDTVPCTTV
                     VGVAENVKSQDLDQDPGYFYYVSADQFAPDQGGLFVRTAMPGVAARDILRKRLQAVMP
                     GPSYVNVTPLSDVFGNETRAWNLGATMFTVFGLLALALAAIGLYSVVAYNVAQRTHEM
                     GVRVALGAEVRDLVRLVLREGLLLASVGVVLGGVVAVLAAPALKALLFQEQPRDPVVL
                     IGVAVTLLAVATVASLVPARRASRVDPMQALRSE"
     gene            complement(7910..10405)
                     /locus_tag="VFU75_11355"
     CDS             complement(7910..10405)
                     /locus_tag="VFU75_11355"
                     /inference="COORDINATES: protein motif:HMM:TIGR03434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADOP family duplicated permease"
                     /protein_id="HEU5467479.1"
                     /translation="MTFRWETLAQDLRYAVRGLRSKPAFTAAVTLTLALGIGANTAMF
                     SVVDRLLFRPPALLRDPSRTHRIYLAGTYRGHTGYQGTIQFARYLDFTRLTHSFDRLA
                     EVAARPLAIGVGTDAREMQVAAVSASFFGFFDAPPALGRYFTSAEDTTPRGAPVAVLG
                     YGLWETRFGGRSDVLGQTIQIGATLYTIIGVAPRDFVGLWGAEPPAAYVPVTTYGAED
                     GANLHFKGEEWWTTYHWTWANLIAERKPGVSLDEATADLTQAYLKSQDIQWEREPGPH
                     QPRTLSKPHGVLASILSERGPDESAEAKVATWLGGVALIVLLIACANVANLLLARALQ
                     RKREIAIRLALGVSRTRLAGQLLSESLLLALMGGLGGLLIAQFGGAVLRSALLSGTAD
                     AAVISDPRTLMIVGAATLLAGLLTGLAPVFQTRRADLTADLKAGVREGAIHRSRTRVA
                     LLVLQGALSVVLLVGAGLFVRSLRQIRHLPLGFDPDSVSVVSIEWRGLNPDSVTSAAV
                     RRRLFAEAKTIPGVTHVSRQVTMPFWDSWNDVIHVPGIDSTERLGQFFLDAVSPDYFA
                     TMGTRLLRGRGIGDQDVPGAPRVMVVSRAMANVLWPGRDPLGQCVRVGDDTMPCTSVV
                     GVAQNIKSQDLDQDPGFFYYVSSDQFAPTMGGLFVRTAMSSAAAHEVIRRRLQALMPG
                     PSYVTVTPLSDIFGSETKSWRLGATMFTVFGLLALTLAAIGLYSVIAYNVAQRTHEMG
                     VRMALGAEGRDVVRLVLREGLLLASIGVTFGGMAAFLAAPSLNSLLFQESPRDPVVLI
                     GVGVTLLAVAALASLVPARRASRVDPMQALRSE"
     gene            complement(10402..11463)
                     /locus_tag="VFU75_11360"
     CDS             complement(10402..11463)
                     /locus_tag="VFU75_11360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEU5467480.1"
                     /translation="MRFGYWLPVFGGWLRNVPDEGMEATWEYVSRLAVRSEALGYDAT
                     LIAELNLNDIRGPEAPTLDAWSTVAALAAVTKRLELMTAVRPTFHPPALCAKQAATID
                     RMSGGRLTLNVVSSWWAEEARQYGVEFDQHDARYARTEEWLAVVSGMWSEPRFSYAGR
                     YYRIENAILEPKPISSPRPVLYAGGESDAAKTLIAQACDAYLMHGDPVERIAAKVTDM
                     RDRREKAGKPPLRFGVAGYVVCRGTDAEARREVARITDVHSNAASYANYQDWLTNTNL
                     ERRVSLEEYSVSNRGLETGLVGTPSRIAERLLELEKAGVDLVLLQFSPQLEEMERFAE
                     EVIPLVRATKTPAGASYHG"
     gene            complement(11466..12428)
                     /locus_tag="VFU75_11365"
     CDS             complement(11466..12428)
                     /locus_tag="VFU75_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467481.1"
                     /translation="MSASTDRPIAIYYEHPDWFRPLFQELDRRSVPYEKIDAANHRFA
                     PGNGRPRYSLVFNRMSPSAYLRGSSNAIFYTLHYLAFLKRAGIPVVNGYDAYVTETSK
                     ALQLDLLRRLGLPFPKAEVINHPSQATAAAKNLQFPVVVKPNIGGSGAGVTRFDTPAD
                     LATAISAGTVDLGIDQTALVQEYIPVEDQGIVRVEVLGGKFLYAIRVFADADSFNLCP
                     ADVCQTVGGVELTRAACPVDAPKNGMRVEGYTPPAEVIAQVERIMKEASIDVGGVEYL
                     HDARDGHRYFYDINALSNFVADAPRVVGFDPFARLVDYLERKAV"
     gene            complement(12668..13093)
                     /locus_tag="VFU75_11370"
     CDS             complement(12668..13093)
                     /locus_tag="VFU75_11370"
                     /inference="COORDINATES: protein motif:HMM:NF024297.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5467482.1"
                     /translation="MKAFVPLALALWFGPQLTAQTAADTAGIVATAHNYIDGWYTNDG
                     PRMESALHSHLAKRLVWDDSTKHSHLVDMTALELVQGTVRHPKGDGQHRNEVKILDIF
                     GNVAMVRIDADTWVDFLQETRWNGKWVIMNVMWENRPGM"
     gene            13268..14353
                     /locus_tag="VFU75_11375"
     CDS             13268..14353
                     /locus_tag="VFU75_11375"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEU5467483.1"
                     /translation="MSFLSRRGVRIVLIWLGVAAFFITQNVFMKLARHVAIDWQWDVF
                     HEIVYAMVWAACTPVILWAADRWRLEPGAPAGFVWPHLALMPVIGLVQITGTYTTHFL
                     VLSAIGYPPAQPLGKLIMSGLIWGVFTGSLYYWVIIGVYMAFLYPRLYQAQRAAAAEL
                     QSQLSRAQLDALRLQLHPHFLFNTLNAIAALAPGDPGRAQRMLARLGDLLRHTLDTEA
                     AEIPVEQELKLLAPYVEIQRLRFEERFRYSERVAPETLPALVPALFLQPLVENAVQHG
                     VSKRPEGAAVSLAIQRMGEQLVLEVIDDGPGPRPGNGGKDGNGIGLANTRARLAQLYP
                     GANEVDLAAGPEGGARLTVKIPYRTDH"
     gene            14353..15111
                     /locus_tag="VFU75_11380"
     CDS             14353..15111
                     /locus_tag="VFU75_11380"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5467484.1"
                     /translation="MRVVIVDDEPLGRLAVRQHLEGIPGAEIVAECPEGKSAIEAIVR
                     ESPDVVFLDVQMPGLSGFDVLEGIGAEAVPAVVFVTAFDRYAVRAFEAHAVDYLLKPI
                     DPDRFREAYARAATAADREGRERMAGRLEALLERLAERPREPSAPVERLVVRHEGRLL
                     FVPVAGIEWVEAAGNYVKLHAGPAVHVMRQTMERLAARLGERFVRIRRSALVNVAAIR
                     ALEPHGKGSYVVFLKDGTQLISSRYAGKILRKLA"
BASE COUNT         2575 a         4846 c         5218 g         2474 t
ORIGIN      
        1 ctcccgccac ccccgccgat ggcgacctcc ctccagttca ccgcccagcc gcagtcggcg
       61 caaacgggcg ggtcgctcgg cacgttcacc gtggccgccg tcgattcggc gggcacggtc
      121 gtgacggact tcaacggcac cgtgacgctc gccatcggga ccaacccggg tggcgggaca
      181 ctctccggca ccgcgtcggt ggcgctcggc aacggggttg cgacattctc cggcttgagc
      241 atcgacaagg cagggaacgg gtacacgctg accgcgtcga cctcgggcct gaagagcgcg
      301 gccagctcgc ccttcaacgt gaccacgcca ccgccgacta cgggtagcct gacggtgacg
      361 acggccacga cggggtcgaa ccttccgacg agcggctata cgatcagcct cgacggtggc
      421 tccgggcagg cgatcgggat caacggtagt ctgaccctga acaacctgac ggcggggagc
      481 cacaccgtga cgatcagcgg gctggcatcg aattgcacgg tcggtggcgg cacgtcgcag
      541 gcggtgaccg tgccggcggg tggatcgacg tccgcatcct tcgcgatcac ctgcgtggcg
      601 ccgccaccga ccaaccatcc gccggtggtg aatgcgggtg ggaaccagtc ggccgtcgaa
      661 gcggtgctgt atcagctcaa cgcctcgttc acggatgtcg acggagatgg tccttggacc
      721 tacgacgtca actggggaga cgggacgtct tcgtcgggga cgctctcggc acagggagca
      781 atcgggccga gtcacaccta cgtgctaccg agcgactata cgatcacggt gacggtgact
      841 gacgcccacg gtgcgtcggg tgccgattcg aaaaccctgc acgtcactct ttagaccgca
      901 cgctccactg ccacatgccg gacatggttt tggtggacac cgggtggccc aggagccgcg
      961 cgagcgtcac gatctggccg cgatggtgcg cgtcgtggct catgtagtag gcgagaaaca
     1021 tcgccgcatt gcccccggaa aagccgtcca cccgcccatt ccccttcaga cactgcgtca
     1081 cgatccgtag caccgccttt cgtttggcac ccaggacctt cacggcctgc ttctgcgtga
     1141 cggagaagcg atcgagctcg gggggcacgg ggacctttgg agcggcgcgg cgaagataga
     1201 tcaacccgca gttgtgcacg tgggcgacca gcgcggcgat ggtacggacc ttcgggcccg
     1261 gcaccttggc gcgccacagg gaggggtgga gatgctccac caggtactgg ttgatgcggg
     1321 cgctcgccga gtaggcggag agcagggtgg cggtggggtc gatcggcatg gggtcctcga
     1381 ctggggatgc cccaatctta cggtgagaac atggcttgcg cgccttcggc gcagatcacg
     1441 atttcgaaaa agcaggtcct tcgcgccttc ggcgcagctc acaatgtcga aaagcaggtc
     1501 cttcgcgcct tcggcgctca ggatgacaag ccatcggttt catgagcaag agtgggaagg
     1561 gagacaggtc ctgcaagcag gacctgtctc ccttcccact cctttaccac gcaaggcgca
     1621 cgcattgtca tcctgagcga gcgcgcgaac gcgcgcgacg cgaaggacct gcttgccctg
     1681 cattgtcgct gcacagctgc gccacgaagc gctcgccctg agcgaagcga gggacccgct
     1741 tcttgatggg tgagtcgcgc ctaggtctcg ggcacgagcc ccatctcgtc caggaactga
     1801 ttgcctccgg caggcatccc gaatcgctgg tcggcccggg tgaggatcaa tcggtccttc
     1861 gcccgggtca tgccgacgta caacaagcgc cggccttcct gaatctcgtc ctccacctgg
     1921 tcgcgcatgg tccggtaccc cggaatctga aagtcctccg cccccacgat gtagacccgt
     1981 gagaactcga gccccttggt ggaatggagc gtgagcaggg tgacgcgttg ggggtcggtg
     2041 ctcactccgt cccgcgacga cagtgccacg cgctcgagga gcttcgtgat cccctcggcg
     2101 atcggcaggg cggcatcttc tcccatgagt gcccggaggc gggccaccgc ccgcggctgg
     2161 gccagctcgg ggtcgtagtc tccctgcagg cgggcccgca gtgccgcgcc tcccagcccc
     2221 ttcacgacct catccaccgg cggtcccagg cgggtccgtt cgtgatccac ttcgtagtac
     2281 tcgagcgcgt ccgagaaccg gccgagggcg cggaaccgtc cgatgtcgaa gagcgtcttg
     2341 ccttcgccgg ccgcacgggc cgccggctcg aaggtgagcg ccacggccag atgccccagg
     2401 atttcgacga ggacggcttt gcgcgcgcgg agcaagcgaa ggcgggtgag gtgctgaaac
     2461 acggaggcca atgccagcgc gtcatcgagc gcgtgatgcg catcgccgga atcgacgttg
     2521 aagcgggccg acaggtcggt cagcttggcg ctttccttga acagggagcg cgcgaggggc
     2581 agggtgtcga agaagaccat gtcgctggtc tgctcttcgc ccgcgagccg gcgcaggatg
     2641 ggcacgtcgt actgcagtcc attgtgcgcg accatcagat catttccgac aaactccttg
     2701 aagtcgggcc atacctcggc gaaggagggc gcatcggcga gatcggcatc cgagtagcca
     2761 tgcaccttgg tcgcggacga cggaacgcca tgctcgggcc ggacgagtct ggagaacttt
     2821 tccacgactt gccccctgac gacgcgcacc gccgccaggt cgacaatctc ggcggtatcc
     2881 ggatccgtgc cggtcgtttc cgtgtcgaag gtgacgaagc gatcgaacga ttcggtgctc
     2941 tcggcggcat gcgcaagctg gagggctttg aatacggtca gggcgagccc agaggagccc
     3001 gcgctgtccg gaccgatgag gacgtcatcg ggcccggccc gccggttcgc gtcgggtgtt
     3061 ccgacgttcc gaagcccggc ggcgaccagc atcgcccgca aggcgaggcc caacccgttc
     3121 atgggttcga cgaccgcttc gccggttcct gccatgaccc gcttgaggcc ctcggcgagg
     3181 cgcacggcct ccgggaggtc cgcggggtcg gtcaggtcgt cgtgacgctc ttccagcacg
     3241 ttccggtagg gcgtgaggcg ttcggagagg agctcgtcca cgagcccgcc cagcgtgcgt
     3301 tcggagcggc tcagcgcctc gaagttttcg cactggaaga cgaactgcca gagtttcttg
     3361 gcatcggggt ccttccgagg ccttttttgc gcgagggagc gaacggccgc gagcacgtca
     3421 cccgcccggg tgccggcctc cacttccagg tacaggtcgg ccgggagcat cgcctcggcc
     3481 aatgaaagga tggccgcttg atcggtcgga tcccggacga tccggagcgc ccggatgacc
     3541 gggccgacga catcgtcttc gttgagggat cgcccgcgtg cgaggcggca ggggacgccg
     3601 gcgcgcagca gatgactttc gagcaagtct ccgacttggt gcctgcgata gagcagggca
     3661 tagtcgcccc agtctccgcc gttcgccgcc atgtcctcct tgatgtccga gatgagccag
     3721 ctcgcttcgg actcctcgtc cccgaagccc agggcgcgaa cggggtacgc cgcatcgcga
     3781 tcggcggaca gctgcttgtg gaagagctgc gggttctctt cgaccagccg ccgggcgacg
     3841 tcgaagatct ggcgggagct ccggcaattc cggtcgagca cgatgggcgt gacgccatag
     3901 tcccggcgaa aggaatccag catcttcggg tcggcgcccg tccagctgaa aatcgactga
     3961 tcctcgtcgc cgacggcgaa gaagttccgg tgcggggcga ggcgcttcag gatggcgtac
     4021 tgcatgggac tcagatcctg gaactcatct accaggagat agtcccagcg cgccgagatt
     4081 tcgtcggcga cgaccgggtg gttgcggagc aacgtgtccg tgcgaacgat gatgtcgtcg
     4141 aagtcgacca gattccggcg gatgaggtag tccgagtaat cgcgaaaggc ctgctcgtcc
     4201 ttggcggaga gcttgaagtc ctgcatccgc cggcgggaga agcggttcaa aagggacgtg
     4261 cgctctttgg cgtagacctt gaggcgagcc aggatcgcga gctggtacgg ctcatccgct
     4321 acgccgaatc cccgtgccaa tccgatctgg tcggcgcgct cacggagcaa gtgcaggcag
     4381 agcccatgca gggtgccccg ggtgagctcc tcggcccggt cgcccagcac cttgctcagg
     4441 cgaacggcga tctcttcggc tgccttgttg gtgaaagtga cgacgcagat ccggtcggga
     4501 gcgattccct ggttcgagat caggtactcg atccggctga tgagacagaa cgtctttccg
     4561 gcacccggtc cggctaccac aagaaccgga ccgagcgggg cctcgatggc ctttcgctga
     4621 ttctcgagcg gcgtaatgtc catggggata ccgcaagata cgaagtcgga aggcggaacg
     4681 cggagtgcgg aaccgccgca acccaggata aggagtcgcc ggtatggccg tccgccaggt
     4741 gctgctcgcg tccttggtgc cactcgcgat gctcacatca cgtccgcccc atcacgtcgt
     4801 ttcaccggaa gcctggcgag acagcgtcag ggcattcgcg gaagctcatc tgcagcacac
     4861 cgcctggggg ccggcgcacg cccgaaggga ttacgaggtg accgtctggc tggcccggga
     4921 ggagaaggtg cccgtggata cggacgccct ctacgccgcc gcttacctcc acgatatggg
     4981 aggtctgccg ccctacgccc ttcccggcgt cgaccatggc gatcggtcca tccagctggt
     5041 tgacacgctg ttgaacgcct ggggttttcc gatggagaag tcgcccctcg taaaggagat
     5101 catcgaccac caccagtact accgtcccgc ggacaccctc aggatcgcca tgctgttccg
     5161 ggacgcggac attcttgatt tcatgggggc aatcgatgtg gcgaggatcg tgtcgctcac
     5221 gacgcgggaa cggctggctc ccgacttggg tcacgcggtg caagtgataa agaagcagat
     5281 gaccgacatg ccggccctcc tgcagtcggc aaccgccagg aaagagggcc agcgtcgggt
     5341 ggcggagatg cgtgccttcc tcgatgcact ggccctggag agcgacagcc tctcgaactt
     5401 gtagttattc cgaccggagc gcctgcatcg gatcgacccg gctggctcgg cgtgcgggga
     5461 cgaggctggc cacggtggcc actgcgagca gggtgaccgc aacgccgatc aggacgaccg
     5521 ggtcgcgcgg ctgctcctgg aagagcaggg ccttcaatgc cggcgcggcg aggactgcga
     5581 cgacccctcc cagcacgacg ccgaccgacg ccagcagcaa tccctctcgt agcaccaacc
     5641 gcaccagatc ccggacttcg gcgccgaggg cgacgcgcac gcccatttcg tgggtgcgct
     5701 gagcgacgtt gtaggccact acggagtaga gcccgatggc cgcgagggcg agcgcgagga
     5761 gcccgaacac cgtgaacatg gtggcgccga ggttccaggc gcgcgtttcg ttcccgaaga
     5821 cgtcggacaa gggggtgaca ttcacgtacg agggtccagg catcaccgcc tgaaggcgct
     5881 tccgcaggat gtcccgcgcg gcgacgcccg gcatcgcggt acggacaaac aggccaccct
     5941 gatcgggggc gaactggtcc gcggagacgt agtagaaata accgggatcc tggtccaggt
     6001 cctgcgattt gacgttctcc gcgacgccga ccacggtcgt gcacggcacg gtgtcgtcgc
     6061 ccacccggac gcactcaccc agggcttcct tgccgggcca gagcacgttc gccatggcct
     6121 gactcaccac catggcacgc ggcgacccgg cgacgtccga ctcggtgacg ccccgacctc
     6181 gcagcagccg ggtgcccata gtcgcgaagt agtccggcgt caccgcgttc agatagaacg
     6241 accccagccg gtccgtggag tcgatcccgg cgacgtggat gtcctggttc caggagctcc
     6301 agaacggcat cgtgatctgc cgggacacgt gcgtcactcc cggtatggtc ctcgcctcag
     6361 cgagcagccg gcgccgcagg gcgacgcttg ccgcgctgtc gagtttgacg ccacgcatct
     6421 gcagatcgac gaccgagacc gaatccgggt caaagccgag gggcaggtgc cggatatggc
     6481 tgagggagcg gacgaaaagg ccggcgccga cgaggagtac gacggacagc gctccctgca
     6541 gcaccagcaa ggccacccgg gtgcgggacc ggtggatcgc gccttctcgt gcccccgcct
     6601 tgaggtcgcc ggtgaggtcg gcgcggcgcg tctgaaagac gggcgcgagg ccggtgagga
     6661 gccccgcgac cagggtcgcg gctcccacca tgatcagcgt acgggggtcc gtgatgacgg
     6721 tggcctgggc cgttttcggc aacagatacg cgcggagaat cgcgccgccg aactgcgcca
     6781 ccaagagtcc cgccaccccg cccaggaggg ccagcagcag gctttcgctc aggagctggg
     6841 aggccagccg gagacggctg acgccgaggg ccaggcggat ggcgatctca cgcttccgct
     6901 gcagcgcgcg ggccaggagc aggttcgcga cgttcgcgca ggcgatcagc agcacgatca
     6961 gggctacacc gccgagccag gtggcgacct tcgcttcgcc ggattcattt ggcccgcgtt
     7021 ccgagaggat cgatgcgacc aagccgtgag gcttggaaag agagagcgga gtgcggtcag
     7081 gggaggggga ttgcgcgtac tcgacgccaa ggctcttcag atcggcggcg gtgaggtccg
     7141 cgtcggcggc cgcgatggtc acgcccggct tgcgctgcgc gatcatccac gcccagcgcc
     7201 agtggtaggt ggaccaccag ttctcgccgc gcaggcgcag attggcgccg atctcggccc
     7261 cgtacgtggc gatcggaacg aatgccgcgg gtggttcgtc cgcccacaac ccgacgaacc
     7321 cgcggggcgc gaccccgatg atcgtgtagg gcgtcgcccc gatttgaatg gtttgtccca
     7381 atacgtccga gcggccgccg aaccgcgtct gccagtatcc gtatgccagc accgccaccg
     7441 cagcgccctg cgggaaggtg tcttcggcgg tggtgaagta gcgtcctacg gcgggggggg
     7501 cgtcgaaaaa tccgaagaag ctggcgctca ccgccccgac ctgcatctcc cgtgcttccg
     7561 tgccgatgcc gatcgccatg tgacgcgccg tgacttcggc ggtgcgttcg aatgaatggg
     7621 tgaagctggt gaggtcgagg taacgggcgt actgggtact gccggaattg tattcctttc
     7681 cccggtaggt ctccgcgagg tagacgcggt gggtgcgggc ggcgtcccgc agcagagcgg
     7741 gcggccggaa cagcagccgg tccaccacgg agaacatggc agtgttggcc ccgatgccga
     7801 gcgccagagt gagcgtcacc gcgacggtga acgccggttt actacggagg ccgcggaccg
     7861 cataccgcag gtcctggacg aggggttccc agcggagtgt catcttcgct tactccgagc
     7921 gcagggcctg catggggtcc acgcggctcg cgcggcgggc cggaaccagg ctcgccagcg
     7981 ccgcgacggc cagcagcgtc accccgacgc cgatcaagac aacggggtcg cggggggatt
     8041 cctgaaacag aagtgagttg agggacgggg ccgccagaaa agccgccatt cctccgaagg
     8101 tcaccccgat cgaggccagc agcaggcctt cccgcaggac cagtcggacg acgtcacgcc
     8161 cctcggcgcc gagggccatc cggactccca tttcgtgggt gcgctgcgcc acgttgtatg
     8221 cgatcaccga gtagagcccg atggcggcga gcgtgagggc caggagaccg aataccgtga
     8281 acatcgtggc gccgagccgc cacgacttcg tctccgaccc gaagatgtcc gaaaggggcg
     8341 tcaccgtgac gtacgaaggg ccgggcatga gcgcctgcag ccggcggcgg atgacctcgt
     8401 gcgccgccgc gctcgacatc gcggtgcgga cgaacagccc tcccatggtg ggcgcgaatt
     8461 ggtcggagga cacgtagtaa aagaacccgg gatcctgatc caggtcctgc gatttgatgt
     8521 tctgggccac gccgaccacg gaggtgcagg gcatcgtgtc atcccccacc cggacacatt
     8581 gacccagggg atccctcccc ggccacaaga cgttcgccat cgcgcggctc acgaccatca
     8641 cgcgaggagc gcccgggacg tcctgatcgc cgatgccgcg tccccggagg agccgggtgc
     8701 ccatggtggc gaagtagtcg ggcgagaccg cgtcgagaaa gaactgtccg agccgttccg
     8761 tggaatcaat gcccgggacg tgaatgacgt cgttccacga gtcccagaac ggcatggtca
     8821 cctggcgcga cacgtgggtg acgccgggaa tggttttcgc ctccgcgaac aaccggcgcc
     8881 gcacggccgc actggtcacg ctgtcgggat tcagcccccg ccactcgatg ctgaccacgg
     8941 acacggagtc agggtcgaac ccgagcggga gatggcggat ctgccggagg gaccgtacga
     9001 agaggccggc accgacgagc agaacgaccg agagggctcc ctggagcacg agcagggcga
     9061 cacgggttcg ggaccggtga atcgcgcctt cccgcacgcc cgccttcagg tctgccgtga
     9121 ggtcggcgcg gcgggtctgg aacaccgggg ccagcccggt gaggagcccc gccaggagcg
     9181 tcgccgcgcc gacgatcatc agggtccgcg ggtcgctgat gaccgcggca tcggccgtcc
     9241 cggataacag ggcgctccga agcacggcgc cgccaaactg cgcgatcagc aacccgccaa
     9301 gaccccccat cagcgcgagc agcaggctct cactcaggag ctgtccggcg aggcgggttc
     9361 ggctcacccc gagggccaac cggatcgcga tctcccgttt tcgttgcaag gcccgcgcga
     9421 ggaggagatt ggcgacgttg gcgcaggcga tcaacaagac gatcagggcc accccaccga
     9481 gccacgtggc caccttcgct tcggccgatt cgtcaggccc ccgctccgac agaatcgagg
     9541 ccaggacgcc gtgtggcttg gagagcgtcc gtggctggtg cggtcccggc tcgcgctccc
     9601 attgaatgtc ctggctcttc aggtaggctt gggtgaggtc ggcggtagct tcgtcgaggg
     9661 acactcccgg cttccgctcg gcgatcagat tggcccaggt ccagtggtag gtggtccacc
     9721 actcctcgcc cttgaagtgg agatttgccc catcctccgc gccgtacgtc gtgaccggga
     9781 cataggcggc aggaggctcg gctccccata ggccgacgaa atcgcggggc gcgacgccaa
     9841 tgatggtgta cagcgtggcc ccgatctgga tggtctgccc cagcacgtcg ctgcggccgc
     9901 cgaatcgcgt ttcccaaagc ccgtaaccca gaacggcgac cggggcaccc cgcggcgtcg
     9961 tgtcctcggc actggtgaag tagcgcccca gtgccggcgg ggcgtcgaaa aatccgaaga
    10021 agctggcgct gacggccgcg acctgcattt ccctggcgtc cgttcccacg ccgatcgcga
    10081 gcggccgcgc cgccacctcc gccaggcggt cgaaggagtg ggtgagccgg gtgaagtcga
    10141 gatagcgggc gaactggatg gtgccctgat agcccgtgtg accccgatac gtccccgcga
    10201 ggtagatccg gtgcgtgcgg gaggggtccc ggagcagagc cgggggccgg aacagcagcc
    10261 ggtccaccac ggagaacatg gcggtattcg ccccgatccc gagcgccagg gtgagcgtga
    10321 ccgccgccgt gaatgccggc ttgctacgga ggccgcggac cgcatatcgc aggtcctggg
    10381 cgagcgtttc ccaccggaag gtcatccatg ataggacgcg ccagccgggg ttttggttgc
    10441 gcggaccagc gggatgacct cttcggcaaa ccgctccatt tcttcgagtt gagggctgaa
    10501 ctggaggagg accaggtcca cgccggcctt ttccagctcc aagaggcgct cggcgatccg
    10561 gctgggggtg cccaccagtc ccgtttccag gccccgattg gagaccgaat actcttcgag
    10621 gctgacccgg cgctcgaggt tggtattggt caaccagtcc tggtagttgg cgtagctggc
    10681 ggcgttgctg tgaacgtccg taattcgggc cacttcccga cgggcctccg cgtcggtgcc
    10741 ccggcagacg acgtatccag ccactccgaa ccggaggggc ggcttcccgg ccttttcgcg
    10801 gcggtcgcgc atgtcagtca ccttggcggc gatgcgctct acggggtctc cgtgcatgag
    10861 gtaggcgtcg cacgcctgcg cgatcagcgt cttcgcggca tcagattcac cgccggcgta
    10921 cagtaccggg cggggtgacg agatgggctt cggctcgagg atggcattct cgatccggta
    10981 gtaccggccg gcgtaggaga accgcggctc ggaccacatg ccgctgacta cggcgagcca
    11041 ctcttcggtc cgggcatacc gggcatcatg ctgatcgaat tccacgccgt attgcctggc
    11101 ttcctccgcc caccaggatg agacgacgtt gagcgtgagg cggccgccgc tcatccggtc
    11161 gatggtcgcg gcttgcttgg cacaaagcgc cggcggatgg aacgtgggac gcacggccgt
    11221 catcaactcg aggcgcttgg tgaccgcggc gagcgcggcc accgtgctcc aggcgtccag
    11281 ggtcggcgct tcggggccgc ggatgtcgtt cagattcagc tcggcgatga gggtggcgtc
    11341 gtagccgagc gcttcgctgc ggacggcaag gcgggagacg tactcccagg tggcttccat
    11401 gccctcgtcg ggcacgttac ggagccagcc gccgaacacc ggaagccagt atccgaatcg
    11461 catggtcaga ccgcctttcg ttcgaggtag tcgaccagac gcgcgaaggg gtcgaatccc
    11521 acgacccgtg gtgcgtcggc cacgaaattc gacagggcgt tgatgtcgta gaagtagcgg
    11581 tggccgtcgc gagcgtcgtg caggtactcc acgccgccca cgtcgatgct cgcctccttc
    11641 atgatccgct cgacctgcgc gatgacctcg gcgggaggcg tgtagccctc aacgcgcatg
    11701 ccgttcttcg gtgcatccac ggggcaggcg gctcgcgtca gctcgacgcc acccacggtc
    11761 tggcagacgt cggcgggaca gaggttgaag gagtcagcgt cggcgaagac gcggatggca
    11821 tagaggaact tcccgcccag gacttcgacg cgaacgatcc cctggtcttc gaccgggatg
    11881 tactcctgca cgagcgcggt ctgatcgatg cccagatcga cggtaccggc actgatggcc
    11941 gtggcgaggt cggcgggagt gtcgaagcgg gtgacgccgg cgcccgatcc gccgatgttg
    12001 ggcttcacca cgacggggaa ctgcagattc ttcgcggccg cggtcgcctg cgaggggtga
    12061 ttgatgacct cggctttggg gaaggggagg cccaggcggc ggaggagatc gagctgcagc
    12121 gccttggatg tctcggtcac gtaagcgtcg tagccgttca cgacgggaat gcccgcgcgc
    12181 ttcagaaagg cgaggtagtg cagcgtatag aagatggcgt tgctgctgcc ccgcagatac
    12241 gctgagggac tcatgcggtt gaagaccagc gagtagcgag ggcggccatt gccgggagcg
    12301 aaccggtggt tggccgcatc gatcttttca tagggcacgg atcggcggtc cagctcctga
    12361 aagaggggcc ggaaccagtc cgggtgctcg taatagatgg cgatggggcg gtcagttgac
    12421 gcggacacga gtgaggcctc ctctatgggt cttccgagaa aagaaaaagg ccgcgaaccc
    12481 gagaggatcg cggccgattg ccgttgtaac cgtcaccagc tgacgttacg gcgtgatcgc
    12541 ccgctcctcg gcacaacaca tcatgccgga gaggtgacaa cagccgcaca tcatcatggt
    12601 ggtgttcatc gttctggccg gaacgtagcg gcggcgcggg cggggcacaa cccgcccgcg
    12661 ccggaggcta catgcccgga cggttctccc acatcacgtt catgatgacc catttcccgt
    12721 tccatcttgt ttcctgcaag aagtccaccc aggtgtcggc gtcgatgcgc accatcgcga
    12781 cgttgccgaa gatgtccagg atcttcactt cattgcggtg ctggccgtcc cctttggggt
    12841 gacgaaccgt gccttgcacc agctccaggg cggtcatgtc gacgaggtgg ctgtgcttcg
    12901 tcgagtcgtc ccacacgagg cgcttggcga ggtgcgagtg cagggcgctc tccatgcggg
    12961 gaccgtcgtt ggtgtaccag ccatcgatgt agttgtgtgc cgtggcgacg atgccggcgg
    13021 tgtcggcggc ggtctgggcc gtcagttgcg ggccaaacca gagcgccaat gcgaggggga
    13081 caaaggcctt cacggagagc cgtcctggga gaagtgatga cacaaggtcg ggcgccggac
    13141 gtgtcgcgcc aagattcccg tcgccgcagg ggcccgattc gtgacgaacg gggccggagt
    13201 cgtgacggac gggccctgac gaattcatgg ttgcacgggg ggccggctgg tcaccagatt
    13261 gccgcccatg tcattcctct cgcggcgcgg cgtgcgcatc gtcctcatct ggcttggtgt
    13321 cgccgcgttc ttcatcacgc agaacgtctt catgaagttg gcccgtcatg tcgcgatcga
    13381 ctggcagtgg gacgtcttcc acgaaatcgt ctatgcgatg gtatgggccg cctgcacgcc
    13441 ggtcatcctc tgggcggcgg accgctggcg cctcgagccg ggggcgccgg ctgggtttgt
    13501 gtggccacac ctcgcgctga tgccggtgat cgggctcgta cagatcaccg gcacgtacac
    13561 gacgcatttc cttgtgctct ccgccatcgg ctacccaccg gcccagcccc tcggcaagct
    13621 gatcatgagc gggctgatct ggggagtgtt tacggggtcg ctctactact gggtcatcat
    13681 cggcgtgtac atggcgttcc tctatccgcg gctgtatcag gcccagcgcg ctgccgccgc
    13741 cgagctgcag tctcagctgt cccgggctca gctcgatgcg ttacgcttgc agctgcatcc
    13801 ccactttctg ttcaacacgc tgaacgccat cgccgcgctt gctcccgggg accccgggcg
    13861 tgcgcagcgc atgctcgccc gccttggcga cctgctccgt cacacactcg acacggaggc
    13921 cgcggagatt ccggtggaac aggagctgaa actcctcgct ccctatgtag agattcagcg
    13981 tctgcggttc gaggagcgct ttcggtactc ggaacgggtg gctccggaga cgctgccggc
    14041 gctggtcccg gcgcttttcc ttcagcccct ggtcgaaaac gccgtgcagc atggcgtcag
    14101 caagaggccc gagggcgcgg cggtgtcgct ggcgatccag cgaatggggg agcagctcgt
    14161 gctcgaggtg atcgacgacg ggcccgggcc gcggccgggc aacggaggga aggacggcaa
    14221 cgggatcggt ctggcaaaca ctcgggcgcg cctggcgcag ctctatcccg gggcgaacga
    14281 ggtggatctc gcggcggggc ccgaaggcgg cgcccgtctg acggtcaaaa tcccttaccg
    14341 gacagaccac tgatgcgcgt cgtgatcgtc gatgacgagc ccctgggacg gcttgccgtg
    14401 cgccagcatc tggagggcat tcctggcgca gagatcgtcg ccgagtgccc ggagggaaag
    14461 agcgcgatcg aggcgatcgt ccgggagagc ccggatgtcg tctttctcga tgtccagatg
    14521 ccgggcctct cgggattcga cgtcctcgag ggtatcggtg ccgaggccgt tccggcggtg
    14581 gtattcgtga ccgccttcga ccgctatgcc gtccgcgcct tcgaagcgca cgccgtggac
    14641 tacctcctca agcccatcga tccggaccgg ttccgggagg catatgctcg ggcggcgacg
    14701 gcggcggacc gggaagggcg ggagcggatg gcgggaaggc tcgaggcgct gctcgagcgt
    14761 ttggcggagc ggccgcggga acccagcgcg ccggtggagc ggctggtggt gcggcacgag
    14821 ggccgcctgc tattcgtccc ggtggccggg atcgagtggg tggaggcggc ggggaattac
    14881 gtcaagctgc acgccgggcc cgccgtgcac gtgatgcggc agacgatgga gcgtctcgcc
    14941 gcccggctgg gcgagcgttt cgtcaggatc cgacgctcgg cgctggtgaa cgtcgcggcg
    15001 atccgcgccc tggagccgca cgggaagggg agttacgtcg tgtttctcaa ggatggcacg
    15061 cagctcatct cgagccgcta cgcggggaag attctgcgga agcttgccta ggc
//
LOCUS       DASUBP010000296         4656 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_339144, whole genome shotgun sequence.
ACCESSION   DASUBP010000296 DASUBP010000000
VERSION     DASUBP010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4656)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4656)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4656
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339144"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1634
                     /locus_tag="VFU75_11385"
     CDS             <1..1634
                     /locus_tag="VFU75_11385"
                     /inference="COORDINATES: protein motif:HMM:NF036516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467485.1"
                     /translation="APASFTLAMFSHPEYDDRYWRYLDTVRADGATIRRDGDATWSVS
                     HATADVTIHYRLVLPPQSGAQRAAWKPFLTSTGGLVDAPHALLYVVGSERGPSEVRLE
                     LPKGWHVATPLAGDAGHYRARNVAELMEGPFLLGHLREWGFQVANTPHRVFYWDGPDG
                     KPFDTTTFVSHIQAIVRQAGALFGGLPYARYSFLFQDKSYGALEHPDAVTLGAPSADL
                     ASDPDALLEETAHEYFHLWNLMRIRPAGYGGVTYRPRPLSTGLWVSEGFTMFYADLLP
                     RRAGLPLRDSTRARHLERLLARYLENPVFARYSAERISESSYAPDPTFLGDYQGSTHL
                     NGEIIGAMLDMLIRSRTGGRRSLDDVMRGLASDSAGFTTTSVQRLVGAVCGCDVEPFF
                     DRYVRGAGVIDVNEYLHLIGLRAEVDTVTAVDDSGRAVPDVRIWSYQPVGAQRPLLLL
                     NTPLSQWGRAGFHSGDTLVTIGDSAMTDIATFRRTIRRFRVGDTVQVTVRRAGGGFTA
                     RVPVTSFQHPVVRIVADAGAPASVRKLRERWEMGR"
     gene            complement(1725..2852)
                     /locus_tag="VFU75_11390"
     CDS             complement(1725..2852)
                     /locus_tag="VFU75_11390"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEU5467486.1"
                     /translation="MKKLLKWLAWPFVVLMALARWAGGTFLISALALFPVALVAELMF
                     RKHHPWKVHLLTAAGASLVIGAFVGAAAWLGLKFPDLSIPGLALKDDAPGVRKGATRG
                     ESLTAQGVAALRAAHQLVDDLPRILDDPISVRLVGREFGERLAAHPERIREPRVTALR
                     SHVLLRSRYAEDRLALAVQSGVRQCVIIGAGLDTFAYRQPPAPEGFRVFEVDHRASQE
                     TKRDLLAKAEIAIPENLTYVELDIEKLPLKDTLVAAGVDPAQPAFFSCLGVLVYLTKD
                     AVRDLFRMVTGFPAGSELVFTYSGNESADEDPASIAARAKLLGEPWLTRFSPEELVGE
                     LRQAGFHAIEFLTREAAARYMGNRNDGLEPPRRARIVSAKV"
     gene            complement(2849..3712)
                     /locus_tag="VFU75_11395"
     CDS             complement(2849..3712)
                     /locus_tag="VFU75_11395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467487.1"
                     /translation="MPRIALATYAAFPDLPPDDQIFQRALDRRGAQTVPLLWDAQVDW
                     SSFDVVVVRSCWDYHLRHNEFLAWIDRVERGGAILHNAPSILRWNSDKRYLRDLEQAG
                     IDVVHTRWTDAAREPPSLASVLAEEGWHDVVIKPAISASATDTWHVPAGKSAEWEDRF
                     RAMVRRGAVMIQPFVSEVARDGEWSLVFIGGEFSHAMLKHPAPGDFRVQTEHGGSQRR
                     AGPDPGLVRAAQSVLSCAPEATSYARVDGCVVDGHFRVMELELLEPTLYFTEGPAAAD
                     RLADLVLARRP"
     gene            3794..>4656
                     /locus_tag="VFU75_11400"
     CDS             3794..>4656
                     /locus_tag="VFU75_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HEU5467488.1"
                     /translation="MSLRPVERIVESQPTMEGAGVRLRRAFGFGDTKEYDPFLLFDDF
                     RNDRPADYLAGFPWHPHRGIETITYVLKGTVDHGDSLGNRGSLGAGDVQWMTAGSGIL
                     HQEMPKGDPKGLMHGFQLWANLPKRLKMTDPRYQDVQAKDIPEVTDDDGTLVRVVCGD
                     FWGKKGPIEGVAADPRYLDVFVPPGKKKTLPVENGRHAFAYVFDGSGSFRDASQPRAV
                     LTEGAGPEAIAKYDKAGNRSLVLFGGGDEVTVQAGDEGIRFLLVSGRPLEEPVAWYGP
                     IVMNTNDELRQA"
BASE COUNT          790 a         1531 c         1552 g          783 t
ORIGIN      
        1 atgcgccggc ctcgttcacg ctcgcgatgt tcagtcaccc cgagtacgac gaccgatatt
       61 ggcgttatct cgacacggtc cgggcggatg gcgcgacgat tcgccgcgac ggtgacgcga
      121 cgtggagtgt gagccacgcg acggcggacg tcacgatcca ctaccgcctg gtcctacccc
      181 cccagagcgg tgcgcagcgc gcagcctgga agccctttct cacgtccacc ggtggcttgg
      241 tcgacgctcc gcacgcgttg ctctatgtgg tggggtcgga gcgagggccg tccgaggtac
      301 gcctcgagtt gcccaagggc tggcatgtcg cgacccccct ggcgggcgac gcgggccatt
      361 accgggcacg aaacgtcgcg gagttgatgg agggtccgtt cctcctgggg cacttacggg
      421 agtggggctt tcaggtcgcg aacacaccac atcgcgtctt ctactgggat gggccggacg
      481 ggaaaccgtt cgatacgacg acgttcgtca gtcacatcca ggccatcgtg cgccaggcgg
      541 gcgcgttgtt cggcggtctc ccgtacgcgc ggtactcttt tctctttcag gacaagtcct
      601 acggcgccct cgaacaccct gacgcggtca ccctcggcgc gcccagtgcc gatctggcga
      661 gcgaccccga cgcgctcctc gaggaaacgg cgcacgagta cttccatctc tggaacctga
      721 tgcgcatcag gccggccggc tacggtggag tgacgtatcg gccgcgaccg ctctcgaccg
      781 gtctctgggt gagtgaaggc ttcaccatgt tctacgcgga cctgctcccg cggcgcgccg
      841 gccttccact tcgcgattcc actcgcgccc gccacctcga acgcctgctc gcccgctatc
      901 tcgagaaccc ggtcttcgcg cgctactcgg cggagcggat cagcgagtcg tcgtacgccc
      961 cggacccgac cttcctcggg gactatcagg gaagcaccca tctgaacggc gagatcatcg
     1021 gcgcgatgct ggacatgctc atccgttcgc gcaccggtgg ccgccgttcg ctggacgacg
     1081 tgatgcgggg cctggccagc gattccgcgg gattcaccac gaccagcgtg cagcgcctcg
     1141 tcggagcggt ctgcggttgc gacgtcgagc catttttcga tcgctacgtt cgcggcgccg
     1201 gcgtcatcga tgtcaacgag tatctccatc tgatcgggct tcgagcagag gtcgacaccg
     1261 tcactgccgt ggatgacagc gggcgtgccg ttcccgacgt gcggatctgg tcttatcagc
     1321 ccgtgggcgc tcagcgcccg ctcctgctcc tgaacacacc gctgagtcag tggggacggg
     1381 cgggattcca ttccggggac accctggtga ccatcggcga ttcggcgatg accgatatcg
     1441 ccacgttccg ccggaccatt cggcgcttcc gggtggggga cacggtacag gtgaccgtgc
     1501 gccgggcggg cgggggattc acggcccgcg tgccagtgac gagtttccaa cacccggtcg
     1561 tgaggatcgt tgctgatgcg ggagctcccg cgagcgtccg gaagctgcgg gagcggtggg
     1621 agatggggcg atgagcccct ccttcgttaa ccccgggctc gcgcccgggg ttatgtaaag
     1681 accggggtgg ccatggtagt cgatgacccc ggacgcaagc acgcctacac cttcgcgctg
     1741 acgatcctcg cccgacgggg tggttcgagc ccgtcgttcc ggttgcccat ataccgggcg
     1801 gccgcctcgc gggtcaggaa ctcgatggcg tgaaatcccg cttgccgtag ttctcccacc
     1861 agctcctcag gcgaaaagcg ggtcagccaa ggctcgccca acaacttggc gcgcgcggcg
     1921 atcgacgcgg gatcttcatc ggcgctctca ttgcccgaat aggtgaagac cagctctgat
     1981 cccgcgggga atccggtcac catgcgaaac aagtcccgca cggcgtcctt ggtcaggtaa
     2041 accaggaccc ccaggcacga gaagaaggcc ggttgggccg ggtcaacccc ggcggcgacc
     2101 agagtgtcct tgagtggcaa cttctcgatg tcgagctcca cgtacgtgag attctcgggg
     2161 atcgcgatct cggccttcgc gaggaggtcg cgcttggtct cctggctggc tcgatgatcc
     2221 acctcgaaga ctcgaaaacc ctcgggcgca ggaggctggc ggtaggcgaa cgtatcgagt
     2281 cccgcaccga tgatcacgca ctgccggacc cccgactgga ctgccaaggc gagccggtcc
     2341 tcggcatagc gtgaccggag cagcacgtgg ctccgcaggg ctgtgacccg gggctcgcgg
     2401 atccgctccg ggtgggcggc gaggcgctcg ccgaactcgc ggccaacgag ccggaccgag
     2461 atgggatcgt cgaggatccg agggagatcg tcgaccagtt gatgtgcggc tcgcagtgcg
     2521 gccacgccct gggccgtgag tgattcccct cgcgtcgcgc ctttcctaac cccgggcgca
     2581 tcgtccttta gggcaagccc ggggatggag agatcaggga acttgaggcc cagccaggca
     2641 gccgccccta caaaggcgcc gatcacgagc gaggcgccgg cggccgtgag caggtggacc
     2701 ttccagggat ggtgctttcg gaacatcaac tcggccacga gtgccaccgg gaagagtgcg
     2761 agggccgaga tcagaaacgt cccgccagcc caccttgcca gggccatcag cacgacgaag
     2821 ggccaggcga gccacttcag aagcttcttc atggacgcct cgcgagcacc aggtccgcca
     2881 ggcggtcagc ggcagccggc ccttccgtga agtagagggt cggctccagg agctccagct
     2941 ccatgacccg gaaatgcccg tctaccacac aaccatcgac ccgcgcatac gaggtggcct
     3001 ccggggcgca cgacagtacg ctctgcgcgg cgcgaaccag cccgggatcc gggccggcgc
     3061 ggcgttggct cccgccatgc tcggtctgca cccgaaagtc gcctggcgcc ggatgcttca
     3121 gcatggcgtg tgagaactcg cctccgatga acacgaggga ccactccccg tcgcgcgcaa
     3181 cctcggatac gaatggctga atcatgaccg caccgcggcg caccatcgcg cggaaccggt
     3241 cctcccactc ggcgctcttc ccggccggca cgtgccaggt atcggtggcc gaggctgaga
     3301 tcgcgggctt gatgacgacg tcgtgccagc cttcctcggc aagcaccgaa gcgagtgacg
     3361 gcggctcccg ggcggcgtcg gtccagcgcg tgtggacgac gtcgatgccc gcctgctcga
     3421 ggtcgcgcag gtagcgcttg tcggaattcc accgcaggat ggagggggca ttgtggagga
     3481 tcgctccgcc acgctccacg cggtcgatcc acgcgaggaa ctcattgtgt cgcaggtggt
     3541 agtcccaaca ggaccggacc accacgacgt cgaagctcga ccagtccacc tgtgcgtccc
     3601 agaggagcgg gacggtctgc gccccgcgcc gatcgagggc acgttggaag atctggtcat
     3661 ctggcggcag atccgggaaa gcggcgtagg tcgcgagggc gatgcgcggc atcgcgagaa
     3721 tctagtcggg aacttgcgga ctgacttgcg tgttcatgtg gagctgcagt cgacttacca
     3781 ttcggaggtt caggtgtctc ttcgtccggt tgaacgcatc gtcgaatccc agcccaccat
     3841 ggagggcgcc ggcgtgaggt tgcgccgtgc cttcggtttc ggcgatacca aggaatacga
     3901 tccgtttctg ctgttcgacg atttccgcaa tgaccgtccc gccgactacc tggcgggctt
     3961 tccgtggcac ccccaccgcg gcatcgaaac catcacgtac gtgctcaagg ggaccgtcga
     4021 ccacggcgac tccctcggca atcgcggctc gctcggcgcc ggggacgtgc agtggatgac
     4081 cgctggcagc gggattctgc atcaggagat gcccaaggga gaccccaagg gcttgatgca
     4141 cggattccag ctctgggcca atctgccgaa gcgcctcaag atgaccgacc cgcgttatca
     4201 agacgtgcag gcgaaggaca tccccgaggt caccgacgac gacggcacgc tggtgcgggt
     4261 ggtctgcggc gatttctggg gcaagaaggg gccgatcgaa ggggtcgccg ccgatccgcg
     4321 gtacctcgac gtattcgtcc cacccgggaa gaagaaaacc ctgcccgtcg aaaatgggcg
     4381 tcatgcattt gcctacgtct tcgacggctc cggcagcttc cgcgacgcct cgcagcctcg
     4441 cgccgtcctc accgagggtg ccgggccgga agcgattgcc aagtacgaca aggccggcaa
     4501 ccggtcactc gtgctgttcg gcggtgggga tgaagtcacg gtccaggcgg gcgacgaagg
     4561 cattcgcttt ctcctggtct cgggccggcc actggaggag ccggtggcgt ggtacggtcc
     4621 gatcgtgatg aatacgaacg acgagctgcg gcaggc
//
LOCUS       DASUBP010000297         3021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_611408, whole genome shotgun sequence.
ACCESSION   DASUBP010000297 DASUBP010000000
VERSION     DASUBP010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_611408"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="VFU75_11405"
     CDS             complement(<1..597)
                     /locus_tag="VFU75_11405"
                     /inference="COORDINATES: protein motif:HMM:NF017970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HEU5467489.1"
                     /translation="MAKVTCSLCGTSVDSRTMVGSQKLEPAIVNLIKRDRPEWPGTRG
                     ICQNCREQYRAKKFLGYLEDEFDKISEMEKSLVTRLAHRGRVSRLVNQEFEAQMTFGE
                     HVADKVAQFGGSWTFIGIFGGILVVWMVINSIFLLKKPFDPYPYILLNLVLSTLAALQ
                     APVIMMSQNRQAEKDRLQAKQDYEINLMAEIEIRDLHDK"
     gene            complement(609..2696)
                     /locus_tag="VFU75_11410"
     CDS             complement(609..2696)
                     /locus_tag="VFU75_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEU5467490.1"
                     /translation="MSRAPAPPTSPTAPPFQYPPTRKSSQVDDYHGTRVPDPYRWLEE
                     VDSPETRAWVEAQNAITFAFLEGIPQRQAIHRRLTELWSYERFGVPAHKAGRWFYFRN
                     DGLQNQAILYRADALDGPPTVLLDPNLLAADGTTALGVTALSEDGRLLAYSVAESGSD
                     WNVIRVRRVDTGVDLSDELRWVKFSSIAWTHDGLGFYYSRYPVPATDGDPLLEVNKNH
                     KLYYHRIGTTQDADRLIWDRPDEPDWGVSAEVTEDGRYLVMSIWLGTDRRNRLYYLDL
                     EDPGKVVHLLDAFDAAWGFVGNDGPVFYLVTDKDAPRRRVVRIDTSSRDLTPSVIVPE
                     QEEALESAHLSRDVLVVSYLHHAHARMRRFRLTGEELAAVALPALGSVAELSGERRSP
                     TLFFAFTSFLTPTAILSHDVETGETRVWRTPAVKFDASRYETTQVFVTSKDGTELPMF
                     MTGRRDRPVDGNAPVYLYGYGGFGVNLTPAFSAGIIGWLELGGVFAMPVLRGGSEYGE
                     AWHEAGMFERKQNVFDDFFAAAEWLIAHRVTRPGRLAIGGGSNGGLLVGAAMTQRPEL
                     FGAALPAVGVMDMLRFHRFTIGWAWVSEYGSADEAGQFPALLAYSPLHNLRPGTSYPA
                     TLVTTADHDDRVVPGHSFKFAAALQAAQAGPAPVMIRIETKAGHGAGKPVAKMIDEQA
                     DRWAFLVKVLGLA"
BASE COUNT          535 a          977 c          972 g          537 t
ORIGIN      
        1 cttgtcgtgc aggtcccgga tttcaatctc cgccatcaag ttgatctcgt agtcctgctt
       61 ggcctgcagc cggtccttct ccgcctgccg gttctggctc atcatgatca cgggagcctg
      121 cagggcggca agcgtcgaca gcaccaggtt gagcaggatg taggggtagg gatcgaaggg
      181 tttcttcagc aggaagatcg aattgatcac catccacacg accaggatcc cgccgaagat
      241 gccgatgaag gtccaactcc ccccgaactg cgccaccttg tccgcgacgt gttccccgaa
      301 ggtcatctgg gcttcgaatt cctggttgac cagacggctc acccgtcccc ggtgcgccag
      361 ccgcgtgacc agcgacttct ccatttccga gatcttgtcg aactcgtctt cgaggtagcc
      421 gaggaatttc ttggcgcggt actgctcgcg gcaattctgg cagatgccgc gggtgccggg
      481 ccactccggc cggtcgcgct tgatgaggtt cacgatggcg ggctcgagct tctgagaacc
      541 gaccatcgtg cgggagtcca ccgaggtgcc gcagagggag caggtgactt tggccatggt
      601 gcaggaagct acgccagccc cagcaccttc actagaaagg cccaacgatc ggcctgttcg
      661 tcgatcatct tggccaccgg cttgccggca ccatgcccgg ccttggtttc gatgcggatc
      721 atcaccggcg ccggtcccgc ctgggcggcc tggagggcgg cggcgaactt gaacgagtgc
      781 ccagggacca cgcggtcgtc atggtcggcc gtggtcacca aggtggcggg atagctggtc
      841 ccggggcgca ggttgtgcag gggcgagtac gcgagaagcg cggggaattg gccggcctcg
      901 tccgccgagc cgtattcgga cacccaggcc cacccgatgg taaaccggtg gaagcgcagc
      961 atgtccatca cccccacggc cggcagggcg gccccgaaca gctcgggccg ctgagtcatc
     1021 gcggcgccca ccaacagccc gccattcgac cctcccccga tcgccagccg gcccgggcga
     1081 gtgaccctat gggcgatcaa ccactcggcc gccgcgaaga agtcgtcgaa aacgttctgt
     1141 ttccgctcga acattccggc ttcatgccag gcctcaccgt attcactgcc cccgcggagc
     1201 accggcatgg cgaagacccc accgagctcc agccagccga tgatcccggc ggagaacgcg
     1261 ggagtgagat tcacgccgaa cccgccgtac ccatagaggt agaccggcgc gttcccatcc
     1321 accggacggt cccggcgtcc cgtcatgaac atcggcagct cagttccgtc cttcgaagtc
     1381 acgaatacct gggtggtctc gtatcggctc gcatcgaact tcacggctgg cgtgcgccaa
     1441 acgcgcgtct ccccggtctc cacgtcgtgc gacagaatcg ccgtgggggt gaggaatgac
     1501 gtgaaggcga agaagagcgt gggggaacgc cgttcgcccg acagctcggc taccgatcca
     1561 agagcgggca gtgcgacggc ggcgagctct tcgccggtca ggcggaagcg gcgcatgcgc
     1621 gcgtgggcgt ggtggagata gctgaccacg agcacgtccc gggaaaggtg cgccgactcg
     1681 agcgcttctt cctgctccgg cacgatgacg ctcggtgtca ggtcccggga gctcgtatcg
     1741 atgcgcacca cccggcgacg gggcgcatcc ttgtccgtga ccagatagaa caccggcccg
     1801 tcgttgccga caaagcccca cgccgcgtcg aaggcatcga gcaggtggac gacctttccc
     1861 ggatcttcca aatcgaggta gtagagtcga ttgcgccggt cggtccccag ccagatggac
     1921 atgacgagat agcgcccgtc ctccgtcacc tccgcgctca caccccaatc gggttcgtcg
     1981 gggcgatccc agatcagacg gtccgcgtcc tgcgtcgtgc cgatgcggtg atagtagagc
     2041 ttgtgattct tgttcacctc gaggagcggg tctccgtccg tggcgggcac gggataccgg
     2101 ctgtagtaga acccgaggcc gtcgtgcgtc caggcaatcg acgagaactt gacccaacgg
     2161 agctcgtcgg agagatcgac ccccgtgtcc accctccgaa cccggatcac gttccagtcc
     2221 gacccgctct cggcgaccga ataggcgagg aggcgtccgt cctcactgag ggccgtgact
     2281 cccagggccg tggtgccatc cgcggcaagg agattggggt cgaggaggac cgtcgggggg
     2341 ccatccaggg catcggcgcg gtacaggatc gcctgattct ggagcccgtc gttgcggaag
     2401 tagaaccagc gcccggcctt gtgggcgggc acgccgaaac gctcgtagct ccatagctcg
     2461 gtcaggcgcc ggtgaatggc ctgtcgctga ggaatgccct ccagaaaggc gaaggtgatc
     2521 gcgttctggg cttcgaccca cgccctggtc tcgggcgagt cgacctcttc cagccagcgg
     2581 taggggtccg gaactcgagt cccgtgataa tcgtccacct ggctggactt ccgggtgggg
     2641 ggatactgga acggcggagc ggtgggactc gtagggggag caggggcgcg gctcatcgcg
     2701 ccgaagtcta tcatgattga cgtaacttac aaggcggctt agatttacat catgcttcga
     2761 accttggctc cgctcgcgtc gttgacgcgc aatctgcctt ccgccgcgcg cgtccgggtt
     2821 catctgcctt cgtcgaactc cgatctctca ggctctgcct catcgagcaa ttgctggaac
     2881 caccttggga ccccctcggg ctcttggccc aacgggggcg caaaggattc gtgctttgaa
     2941 gcgagaaatt ctcatctcag gaagccagcg ggaaacccgc gtggcgattc tcgaggacga
     3001 ccgccttgtt gaactcctgg t
//
LOCUS       DASUBP010000298         3521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_176334, whole genome shotgun sequence.
ACCESSION   DASUBP010000298 DASUBP010000000
VERSION     DASUBP010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_176334"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="VFU75_11415"
     CDS             <1..483
                     /locus_tag="VFU75_11415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HEU5467491.1"
                     /translation="EYVGIAKEQGAAVLIGGAKATGKGLERGWFYQPTLFVGVKPGMR
                     VEQEEIFGPALSVIKVKDLDEAIRVNNDVAYGLSSALYSRDVNAAFRALNELDNGITY
                     VNAPTIGAEAHLPFGGVKQTGNGHREGGWAVYDFYSEIKVGYVDYSGKLQRAQIDTEA
                     "
     gene            480..1460
                     /locus_tag="VFU75_11420"
     CDS             480..1460
                     /locus_tag="VFU75_11420"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEU5467492.1"
                     /translation="MRRAAGVLLMLITAACGRHRAPVAWTDTSPHTVTFVSVAPEVRL
                     EVLDWGGAGPNLVFLSGLDDVAHGFDDFAPQFRDHFHVVAITRRGYGASTRTPTGYDL
                     ATRIQDLTAALDSLNIGKAILVGHSMAGDELTGFAGAHPDRVAALVYFDAAYDHSVLG
                     DLIGSYPQQPPSRADSASPKAFQHYLLTTFGMLIPESQIRATEVFDGNGRLSSDVTPG
                     TVDSQMLAGAGHPDYSKVHAPALAIDAVVDSAPQLFPRWSKFDDSTRAQGRRFTAKIT
                     AWGSDQRAMFRKAMPRASVVELHGANHYVFYSNRNDVVREMREFLSHLPG"
     gene            1598..1852
                     /locus_tag="VFU75_11425"
     CDS             1598..1852
                     /locus_tag="VFU75_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467493.1"
                     /translation="MAHTPFESLVIILNAGGSPLEDDALGALDGVSPVWVRVEREGSV
                     ACLAARIRHVESFDDLGVRIRGWGGARGWTVTVAPCGPPR"
     gene            1974..2636
                     /gene="lepB"
                     /locus_tag="VFU75_11430"
     CDS             1974..2636
                     /gene="lepB"
                     /locus_tag="VFU75_11430"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEU5467494.1"
                     /translation="MRTLGMAFVLFILLHTFVLEAFQIPSGSMEHTLLIGDFLFVNKA
                     VYGAVIPGTHARLPGFASPHRGDVVVFPYPRHPDTTFVKRIVGVGGDTLQMVEGHMIV
                     NGVRSAEPYVERTDSLRNDCDESFEWQVAYLADTSAAARASYRACRDDWGPLVVPPHK
                     FFVMGDNRDISLDSRYWGFVDESTIRGRPLIVYWSVDRDAHDHFPWLNDIRWSRLGSL
                     IH"
     gene            2671..>3521
                     /locus_tag="VFU75_11435"
     CDS             2671..>3521
                     /locus_tag="VFU75_11435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HEU5467495.1"
                     /translation="MQLNTARRASFDEGSLDQYLREISQYPLITREEEVLLAQKIKQA
                     DEEALDKLVRSNLRFVVSVAKKYQNQGVSLADLINEGNLGLIRAAHKFDETKGIKFIS
                     YAVWWIRQAILQALAEQSRIVRVPLNRAGTLHRIGKRSSALLQELGREPTVEEIAEGM
                     DISEEEVAKTMSISQAHLSLDAPLTPGEDNKLLDYLPDTQNPGPDDEIFEHALTESVE
                     KVLGTLKDREALILRLYFGLETPEPLTLEEIGARLGITRERVRQIKEKALARLRHVSR
                     ARALDSFL"
BASE COUNT          605 a         1131 c         1154 g          631 t
ORIGIN      
        1 gagtacgtcg gcatcgcgaa ggagcagggc gcggcggtcc tgatcggcgg tgccaaggcg
       61 acggggaagg ggctcgaacg cgggtggttc tatcagccca cgctgttcgt aggggtgaaa
      121 cccggcatgc gggtggagca ggaagagatc ttcgggcccg ccctttcggt gatcaaggtc
      181 aaggatctgg atgaggcgat tcgggtcaac aacgacgtcg cgtacgggct ctccagcgcc
      241 ctctattccc gtgacgtcaa cgccgccttc cgggcactca acgagctcga caacggcatc
      301 acctacgtga atgcccccac catcggcgcc gaggcgcacc tgccgtttgg tggagtgaag
      361 cagacgggga atgggcatcg cgaaggcggg tgggcggtct acgatttcta ctcggagatc
      421 aaagtcggct acgtggatta ctccggcaaa ctccagcgcg cgcagatcga caccgaagcg
      481 tgaggcgcgc cgccggggtc ttgctgatgc tgatcaccgc ggcgtgcggg cggcatcggg
      541 cacccgtcgc ctggacggat acgtccccgc ataccgtcac gttcgtctcc gtggcacccg
      601 aggtccggct ggaagtactc gattggggcg gggctgggcc gaatctggtg ttcctctccg
      661 ggctcgatga cgtcgcccac ggcttcgacg atttcgcgcc acagttccgg gatcacttcc
      721 acgtcgtcgc catcacccgg cgcggctatg gcgcctcgac gcgtacgccc accggctacg
      781 atctggctac ccggattcag gacctgaccg cggctctcga ctccctgaac atcgggaagg
      841 cgatcctggt ggggcattcg atggcgggtg acgaactgac cggattcgcc ggcgcccatc
      901 cggaccgggt ggcggcactc gtgtatttcg acgccgccta cgaccactcc gtgctggggg
      961 acctgatcgg ctcctaccct cagcagcctc cttcccgggc ggactccgct tcgccgaagg
     1021 cgttccagca ctacctgctg accacgttcg ggatgctgat cccggagtcc cagatccggg
     1081 cgaccgaagt gttcgacggg aacggccgcc tcagctccga cgtcacaccc gggaccgtcg
     1141 attcgcagat gctcgcgggg gcggggcacc cggactactc gaaggtgcac gcgccggcgc
     1201 tggccatcga cgcggtggtg gactccgctc cgcagctctt cccccgctgg agcaagttcg
     1261 acgattcgac cagggctcag gggaggcgct ttacggccaa gatcacggcg tggggctccg
     1321 accaacgggc gatgttcaga aaggcgatgc cgcgggcgtc ggtggtcgag ctgcatggtg
     1381 ccaaccacta cgtcttctat tccaaccgca acgacgtggt gcgggaaatg agggagttcc
     1441 tctcacacct cccaggctaa agcctgggcc cggcactgct cgacgcttca gcgtgctgct
     1501 tttccgggcc cacgcttcga gcgtgggaag cgttttccag taccatcccg cacgattcgg
     1561 gtgcaggtga cgctccgcac gcgccgcaat gtgtgtcgtg gcgcacactc ctttcgagtc
     1621 cctcgtcatc attttgaatg ccggcggctc accgctcgag gatgacgcgc ttggcgcgct
     1681 cgacggtgtg tcaccggtgt gggtcagagt cgagcgcgag ggatcggtcg cgtgcctcgc
     1741 cgcgcgcatc cgtcacgtcg agtcgttcga cgacctcggg gtccggatcc gggggtgggg
     1801 gggtgcccgg gggtggacag tgactgttgc gccctgcggg cccccccgct aacgtacgat
     1861 acatgcacga cttgggggct ccccagagtt cgcccagcgc gccactcccg cgtgagtcct
     1921 cgcatccccc aaaaaagcgc cgttcgttct tcgcccgatg gttcgacgcc tggatgcgca
     1981 cgttgggcat ggcgttcgtg ttgttcatcc tcctgcacac cttcgtactt gaagcgtttc
     2041 agattccgag cggcagcatg gagcacacgc tgctcatcgg ggacttcctc ttcgtgaaca
     2101 aagccgtgta cggtgccgtc attcccggca cgcacgcgcg tcttcccggt tttgcgagtc
     2161 cgcatcgcgg tgatgtggtg gtctttccgt acccccggca tccggacacg acgttcgtga
     2221 aacgcatcgt cggcgtcggc ggggacacgc tccagatggt cgaagggcac atgatcgtga
     2281 acggggttcg gagcgccgag ccgtacgtcg agcgcacgga ctcgctgcgg aacgattgcg
     2341 acgagtcctt cgaatggcag gtggcgtacc tggcggacac ctcggccgcg gcgcgcgcca
     2401 gctatcgtgc gtgccgcgac gattggggcc cgctggtcgt gccgccgcac aagtttttcg
     2461 tgatggggga caaccgcgac atctcgctcg attcccgcta ctggggattc gtggacgaga
     2521 gcacgatccg cggccggccc ctcatcgtgt actggagcgt cgaccgggac gcgcacgatc
     2581 attttccgtg gcttaacgac atcagatggt cgcgattggg ttcgctgatt cactgaagtc
     2641 ttcgttcatg cacggaggaa gagggacccc atgcaactga acacggcgag gcgcgcttcg
     2701 ttcgacgaag gctcactgga ccagtatctc cgtgagatca gtcagtatcc gctgatcacc
     2761 cgggaagagg aggtactcct cgcgcagaag atcaaacagg ccgacgagga agccctcgac
     2821 aagctggtcc gatcgaatct ccggttcgtg gtctcggtcg cgaagaagta ccagaaccag
     2881 ggtgtctcgc tcgccgacct gatcaacgag ggcaacctcg gcctgatccg cgcggcgcac
     2941 aagttcgacg agaccaaggg catcaagttc atctcgtacg cggtctggtg gatccgccag
     3001 gccattctcc aggcgctggc cgagcagtca cgcatcgttc gggtgccgct caaccgggcc
     3061 ggcacactgc accggatcgg caagcgctcg tcggcgctgc tccaggagct ggggcgcgag
     3121 ccgacggtcg aggaaatcgc cgaggggatg gacatttcgg aagaggaagt cgcgaagacg
     3181 atgtcgatct cgcaggcgca tctctccctg gacgcgcccc tcacgcccgg cgaagacaac
     3241 aagctgctgg actacctgcc tgacacgcag aaccccggtc ccgacgacga gatcttcgag
     3301 cacgccctca ccgagtccgt ggagaaggtg ctcggcacgc tgaaggaccg cgaagcgctg
     3361 attctccgcc tctatttcgg cctcgaaacc cctgaacccc tcacgctcga agagatcggc
     3421 gcgcgactgg gcattacgcg ggagcgggtg aggcagatca aggagaaggc gctggcgagg
     3481 ctccggcatg tctcgcgggc cagggcgctc gactcgttcc t
//
LOCUS       DASUBP010000299         2114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1184874, whole genome shotgun sequence.
ACCESSION   DASUBP010000299 DASUBP010000000
VERSION     DASUBP010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1184874"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..551)
                     /locus_tag="VFU75_11440"
     CDS             complement(<1..551)
                     /locus_tag="VFU75_11440"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEU5467496.1"
                     /translation="MKTLDKYVLRAWGQVFGIVMLGFPILAIVINITDKFDQYLSRGL
                     TKGQIGLAYIFYMPEMMFLVLPAAVLFATVFTIGNLGRHSELTAAKASGVSFYRIIAP
                     ILIISVVAFVAGVGIGELAPVTSVRKAEILKERAVNSTTSRFNFVYRADSGWVYAAQS
                     LDVGSRTMTEPLFQREGTGGANPT"
     gene            complement(548..2065)
                     /locus_tag="VFU75_11445"
     CDS             complement(548..2065)
                     /locus_tag="VFU75_11445"
                     /inference="COORDINATES: protein motif:HMM:NF015684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HEU5467497.1"
                     /translation="MAQPAVRILSRYLLRQHLAPLGFALGALTALLLLNQVAKQFGQL
                     VGKGLPWGVILEVFVLSIPFIVAMTIPMAVLVAVLHTTSRLAQDNEITAMKAGGVSIG
                     ALLRPLLGGAAVLAVLAFVWNDQLLPRSNHRLRTLLVDIQRKKPSFSLKEEAINEVVQ
                     GQFFLRAARIDPATNRLTDVTIYELGDPERRRIVVADSGRMAYTPGGTDLYLTLMDGD
                     IREVKRAEPLQFQRTFFHTNQIRVAGVSNSLERTQEDDFRSEREMGVCEMAHEVRLAR
                     VDVARSPLEGRAQVLRDLGRLAGIAIPPGIIDTTRADTLPGWYCGFLGKLTHLVLPRE
                     ARAQTPTRLAPQNPPGHVPAPVVAQPGVVYRPEMGLTPGAGLGTALRERGSRQRAATY
                     EVEIQKKLAISSACIVFALLGFPLGLRFSRGGAGLVIGVSVAVFAVYYIGLIVGEDLG
                     DRLIVSPFFAMWTANVIFTVVGVGLLFLVRHEGGHARSSSWGIASWFARIRRRQA"
BASE COUNT          377 a          686 c          712 g          339 t
ORIGIN      
        1 gtcgggttcg cgccgcccgt cccttcacgc tggaagaggg gctcggtcat ggtgcggctc
       61 ccgacgtcca ggctctgcgc cgcgtacacc caccccgagt cggcgcggta cacgaagttg
      121 aagcgcgacg tcgtcgagtt caccgcccgc tccttgagga tctcggcctt ccgcaccgag
      181 gtcaccggcg ccagctcgcc gatgcccacg cccgcgacga acgcgacgac ggagatgatc
      241 aggatggggg cgatgatccg atagaacgac acgcctgacg ccttcgccgc cgtgagctcg
      301 gaatgccggc ccaggttgcc gatggtgaag acggtcgcga agagcaccgc ggccgggagc
      361 accagaaaca tcatctccgg catatagaag atgtaggcca gcccgatctg gcccttggtg
      421 agtccccggg agaggtactg atcgaacttg tccgtgatgt tgatgacgat ggccaggatg
      481 gggaacccca gcatcacgat gccgaagacc tgtccccagg cgcgcaggac gtacttgtcg
      541 agcgtcttca cgcctggcgt ctccggatcc gcgcgaacca gctcgcgatg ccccagctcg
      601 agctccgcgc atgccctccc tcatgccgca ccaggaagag caggcccacg cccacgacgg
      661 tgaagatgac gttcgcggtc cacatcgcga agaacggcga cacgatcagg cgatctccaa
      721 ggtcctcgcc cacgatcaag ccgatgtaat agaccgcgaa gaccgctacc gacacgccga
      781 tcaccaggcc ggcgcctccg cgcgagaacc gaagccccaa ggggaagccc agcagtgcga
      841 aaacgatgca ggccgaggaa atcgccagct ttttctggat ctcgacttcg taggtcgccg
      901 cccgttgccg ggacccgcgt tcgcgcaggg cggtgcccag ccccgccccc ggcgtcagcc
      961 ccatctccgg ccggtatacc acgccgggct gtgccacgac gggcgccggg acgtgtccgg
     1021 ggggattttg gggggcgagc cgggtcgggg tttgcgcacg ggcttcccgc ggcagcacga
     1081 gatgcgtgag ctttccgagg aacccgcagt accagccggg cagggtgtcg gcgcgggtgg
     1141 tgtcgatgat gccggggggg atggcgatgc ctgcgagccg gccgagatcg cggagcacct
     1201 gcgcccggcc ctcgagtggg gagcgggcga cgtccacgcg ggccagccgc acttcgtgcg
     1261 ccatttcaca aacacccatt tcccgctcgc tccggaagtc gtcttcctgc gttcgttcca
     1321 gactgttgct gacgccggcg acccggatct ggttggtgtg gaagaaggtc cgctggaact
     1381 gaagtggctc ggcccgcttc acctcccgga tgtcgccgtc catgagggtg aggtacaaat
     1441 ccgtgccgcc gggggtataa gccatgcggc ccgagtcggc gacgacgatc cgccgccgtt
     1501 cggggtctcc caactcatag atcgtgacgt cggtgagtcg gttcgtcgcc ggatcgatcc
     1561 gcgctgcgcg caggaagaat tgtccctgca cgacttcatt gatcgcttcc tccttgagac
     1621 tgaaggacgg tttcttgcgc tggatgtcca cgagcagggt gcgcagccga tgattggagc
     1681 gggggaggag ctggtcgttc cagacgaacg ccagcaccgc gagcacggcg gcgccgccca
     1741 ggagcgggcg gagcagcgcc ccgatgctga ccccgcccgc tttcatggcc gtgatttcgt
     1801 tgtcctgggc cagacggctc gtggtatgga ggacggcgac caggacggcc atggggatgg
     1861 tcatggcgac gatgaacgga atcgacagca cgaatacttc gaggatcacg ccccaaggca
     1921 gtcccttgcc caccagctgc ccgaactgct tggcgacctg attgaggagc aacagcgcag
     1981 tcagggcgcc gagggcgaag ccgagtggcg cgaggtgctg tcggaggaga tagcggctga
     2041 ggatgcgcac ggcgggttgc gccattatat tcggaactcc aagtcttctc aagggatgcg
     2101 tctcaccccc atcg
//
LOCUS       DASUBP010000300         3720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_843789, whole genome shotgun sequence.
ACCESSION   DASUBP010000300 DASUBP010000000
VERSION     DASUBP010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3720
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_843789"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="VFU75_11450"
     CDS             complement(<1..579)
                     /locus_tag="VFU75_11450"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5467498.1"
                     /translation="MSDLLDILNEELKGKYVLQRELGRGGMATVFYAEDVKHGRGVAI
                     KVLHPDVAASMGQDRFLREIEIAARLQHPHILALYDSGTAGNHFYYVMPFVEGESLRD
                     RLDREKQLGQEDVLKITAQVASALGYAHSRGVVHRDIKPENIMLSGDTAIVADFGIAR
                     SEGAGHSLTQTGTIIGTPTYMSPEQGTGSPDID"
     gene            complement(608..1465)
                     /locus_tag="VFU75_11455"
     CDS             complement(608..1465)
                     /locus_tag="VFU75_11455"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HEU5467499.1"
                     /translation="MRPKSLGILGLGAIGGSLARQAKAAGIPQVLGWSPEPAERVAAV
                     KQGALDDAPHQAAEVVGRVELLVLAAPPAANLEVLERLAPRIAPGCIVTDVGSVKRAI
                     VAKAEALGLAHRFAGSHPLAGTERNGFDASRADLFLGAVVYVTPTEGGDGAAREVAHF
                     WESVCGAHPVILDAAAHDAQLALTSHLPQVVSSLLGLYLSRHLPPGASLGPGAGDTTR
                     LAGSEPGLWTEILLLNRDAVLPALRALQEPLGELERALEAGDRESVKTWLARGSEWRR
                     GLDRSGREP"
     gene            1493..1855
                     /locus_tag="VFU75_11460"
     CDS             1493..1855
                     /locus_tag="VFU75_11460"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5467500.1"
                     /translation="MAYIVMVDDDPTVRRLGAALLKSAGHEVVDRESGDGLESLLAGR
                     RPDLLLLDIQIPGRDGFALLDAVRNVQGDQPLKVVALSGGSAPDERERIHTAGFDGFL
                     PKPIDVRGFAAAVKNYLD"
     gene            complement(1852..2877)
                     /locus_tag="VFU75_11465"
     CDS             complement(1852..2877)
                     /locus_tag="VFU75_11465"
                     /inference="COORDINATES: protein motif:HMM:NF024743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HEU5467501.1"
                     /translation="MKSFWMRWRAELLRGSVIFVVVVGLGLALVSAWHNGVNSLKART
                     GNIIPALAQGFGASFGGDFDAPGRNHGDSWSWHGKLEPGKTLAIRNIAGPVEVTAAPG
                     AETIVTTEKSWLSSDPNSVVVQAVPTSAGTMICAVWPGSTGNCSAGENLNIHSNGRQH
                     NDVAVKFVVQLGRGVKIDVGGISGDVQVEGATAAVSASTISGDLSVQTDSWPVDLNSV
                     SGDISVTTGRPGTEPAKVKSVSGDVKVYLPANPDVSVHATTVSGDVGDDFDIPVQEAK
                     YTNAQSLNGSIGKGGAELSLTTVSGDIKVSKATSVTVWKVQKKGAATTVVAAPTPAPA
                     PAAPAKP"
     gene            complement(2971..3231)
                     /locus_tag="VFU75_11470"
     CDS             complement(2971..3231)
                     /locus_tag="VFU75_11470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467502.1"
                     /translation="MKGTVLALAAVLTLGSTSLGMQGMAVEWATAQAQGALAGAPRAT
                     PGPADQIWMGGTLPAITVEARAYSAVVFNAPVATLSAPRRRA"
     gene            complement(3327..>3720)
                     /locus_tag="VFU75_11475"
     CDS             complement(3327..>3720)
                     /locus_tag="VFU75_11475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467503.1"
                     /translation="VPDLARSYLMLGRCDDAIRITSRPMTNFYEGVRTLAGIAYAKCG
                     QPERARQVLKELDARRRAGVYVEHYDYGAIYASLGDLDRAFREFNLAVDDREFFLFSV
                     TWDPMWKFLRRDPRFAALTARIALNNKQ"
BASE COUNT          596 a         1292 c         1206 g          626 t
ORIGIN      
        1 gtcgatgtct ggggagccgg ttccctgctc gggactcatg tacgtcggcg tcccgatgat
       61 cgtgcccgtc tgggtcagcg agtgccccgc cccttcgctc cgcgcgatgc cgaagtcggc
      121 gacgatcgcc gtgtccccgg acagcatgat gttctcgggc ttgatgtcgc gatggactac
      181 gccccggctg tgcgcgtacc ccagcgcgct cgccacctgc gcggtgatct tgaggacgtc
      241 ttcctgaccg agctgctttt cgcggtccag gcggtcgcgg agcgactcgc cctccacgaa
      301 cggcatgacg tagtagaagt gattgccggc ggtgccggaa tcgtagagcg cgaggatgtg
      361 ggggtgctgc agcctggcgg caatctcgat ttcacgcagg aatcggtcct gtcccatcga
      421 ggcggcgacg tcgggatgga gcaccttgat ggcgaccccc cggccatgct tcacatcctc
      481 ggcgtagaac accgtcgcca tgccgccgcg acccaactcc cgctggagga cgtacttgcc
      541 cttcagctct tcgttcagaa tgtcgagtag gtcgctcacg gatctccggg caggtggcgg
      601 cgcaacgtta aggctcgcga ccggagcggt ccagtccacg ccgccactcg gacccccgcg
      661 ccaaccaggt cttcacactc tcccgatccc ccgcctcgag ggcccgttcc aattcgccca
      721 gcggctcctg gagggcccgc agtgccggca agaccgcgtc tctattaaga agaaggatct
      781 cggtccagag gccaggctct gacccggcca gacgggtggt gtctcccgcg cccggtccga
      841 ggctcgctcc gggcggcagg tgccgcgaca ggtagagtcc gagcaaagag gacacgacct
      901 gcggcagatg actggtcagg gcgagctggg cgtcgtgggc tgccgcgtcg agaatcaccg
      961 ggtgcgcacc gcacaccgat tcccagaagt gcgcgacctc ccgcgccgct ccatcgcccc
     1021 cctcggtcgg cgtcacgtag accaccgcgc ccaaaaacag atcggcccgc gaggcgtcga
     1081 atccgttgcg ctcggtgccc gcgaggggat gggagcccgc gaaccggtgc gccaacccca
     1141 gcgcctcggc cttcgcgacg atcgcgcgct tcaccgagcc gacgtcggtg acgatgcagc
     1201 cgggggcgat ccggggtgcc agccgctcga gaacttcgag gttggccgcc ggcggggccg
     1261 ccagcaccag cagctccacg cgccccacga cttcggcggc ctgatgcggg gcgtcgtcga
     1321 gtgcgccctg cttcacggcg gcgacccgct ccgccggctc cggactccag cccaggacct
     1381 gggggatgcc ggcggccttg gcttgccgcg ccaacgatcc tccgatggca cctagaccga
     1441 ggatgccgag gctcttgggt cgcatggagg cgaaatgtag gtattgtagg acatggcgta
     1501 catcgtgatg gtggacgacg atccgaccgt ccggcggctg ggcgcggcgc tcctcaagtc
     1561 cgcgggacac gaggtcgtcg accgcgagtc tggcgacggg ctcgagtcgc tcctcgccgg
     1621 acgccgtccc gacctcctgc tcctcgacat tcagattccc ggccgcgatg gcttcgccct
     1681 gctcgacgcc gttcgaaacg ttcagggcga ccagcccctg aaggtggtcg ccctgagtgg
     1741 tggctcggct cccgacgagc gggagcggat tcacaccgcg ggattcgacg gcttcctgcc
     1801 caagcccatc gacgtccgcg ggttcgccgc ggccgtcaag aactacctcg attagggttt
     1861 cgctggagcc gccggagccg gggctggcgt cggtgcagcc acgacggtgg tcgcggcgcc
     1921 cttcttctgc accttccaga ccgtcacgga cgtcgccttg ctcaccttga tgtcgccgct
     1981 cacggtggtc agcgaaagct ccgctccacc cttcccgatg ctgccgttca gcgactgcgc
     2041 gttggtgtac ttcgcttcct gtaccgggat gtcgaagtcg tccccgacat caccgctcac
     2101 cgtcgtcgca tgcacgctca catcgggatt cgccggcaga tagaccttca cgtcacccga
     2161 aacggatttc accttcgcag gctccgttcc cgggcgcccg gtggtcacgg agatgtctcc
     2221 gctcacggag ttgagatcga cgggccacga gtcagtctgg accgagaggt cacccgagat
     2281 ggtgctggcg ctgacggccg ccgtggcacc ctcgacctgg acatcccccg agatcccgcc
     2341 gacgtcgatc ttcacgcccc gcccgagctg gacgacgaac ttcaccgcca cgtcgttatg
     2401 ctggcggcca ttcgagtgaa tgttcaggtt ctcgccggcg gagcagtttc ccgtcgagcc
     2461 gggccacacc gcgcagatca tggtgccggc ggaggtcgga acggcctgca cgaccacgct
     2521 gttcggatcg gagctcaacc agctcttttc ggtggtcacg atcgtctcgg cgccgggcgc
     2581 cgcggtcacc tcaacgggcc ccgcgatatt gcggatcgcg agcgtcttac ccggctcgag
     2641 cttcccgtgc catgaccacg aatcgccgtg gttccggccc ggggcgtcga agtccccgcc
     2701 gaagctcgcg ccgaagccct gcgcgagcgc cgggatgatg ttgccggtcc gggccttgag
     2761 gctattcacc ccattgtgcc aggccgatac gagggccagg ccgagcccga ccacgaccac
     2821 gaagatgacg gagccgcgga ggagttccgc gcgccaccgc atccagaagc ttttcatcgt
     2881 cataaccgcc tcctcctgcg agaaaccccc gcgggattcg taagctgcag aatcctacgg
     2941 gggtttggca gtcccggttt ccgggttcgg tcaggcccga cgcctcggtg cgctcaaagt
     3001 ggccactgga gcgttgaaaa ccacggccga atacgcccgc gcctcgacgg taatggccgg
     3061 aagggtcccc cccatccaga tctgatcagc gggaccgggg gtggcgcgag gggccccagc
     3121 cagggcgccc tgcgcttgcg cggtcgccca ttccaccgcc atgccctgca ttcccagtga
     3181 ggtactgccc aacgtcagga cggcggccaa ggccaacacg gtccccttca tatccagctc
     3241 cctatcgcta aagcgtttga ggtgtctcat cacactcata ggacgcgatt ggggccgaaa
     3301 aggttgcaag aggggtgccg tcgggatcac tgcttgttgt tgagcgcaat ccgggcggta
     3361 agcgccgcaa aacgcgggtc tcggcgcaaa aacttccaca tggggtccca ggtgacgctg
     3421 aacaggaaga actcgcgatc gtccaccgcg aggttgaact cgcggaacgc gcggtcgaga
     3481 tcaccgagcg aggcatagat ggcgccgtag tcgtagtgct cgacgtagac gccggctcgc
     3541 cgccgcgcat cgagctcctt gaggacttgc cgcgcccgct ccggctgccc gcacttcgca
     3601 tacgcgatgc cggcgagggt ccgcacccct tcgtagaaat tcgtcatcgg ccggctcgtg
     3661 atccgaatgg cgtcgtcgca gcggcccagc atcaggtagg agcgggcgag gtccggcacc
//
LOCUS       DASUBP010000301        12283 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_326221, whole genome shotgun sequence.
ACCESSION   DASUBP010000301 DASUBP010000000
VERSION     DASUBP010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 12283)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12283)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12283
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_326221"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1145
                     /locus_tag="VFU75_11480"
     CDS             <1..1145
                     /locus_tag="VFU75_11480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467504.1"
                     /translation="LVIVSAFFPLAPIVDAVTGAVPGDAALTRPALYVILAPLSNTLD
                     ALTFLTIERAEVLLATWVILFILWGVVAGRTRRGRLIRIGLAPIVVFAFAGAAVTLPR
                     PVPALESADSDATIIDFHSHTQASHDGRHGWTDDDLARWHADQGFEAAYVTDHNVIYP
                     GRENGVIALLPGVEWSVYRQHIVALGRAQPIDSTSHALFSKDTPGMLKLFDYLHQQGA
                     LAIASIPEYWENHREDLEQFVTAGVDGFEIVNCAPKALRFSSADRADVLLLAKSHDLL
                     VTGASDNHGWGKVTCVWNLSQVGAKGFRANEVIARPIALAQGDRPIWSAGFDQPWLML
                     RSLTAGERASWLTWITVYLIYMSVPRRQGKRASIGILARDLTPQRD"
     gene            1216..3009
                     /locus_tag="VFU75_11485"
     CDS             1216..3009
                     /locus_tag="VFU75_11485"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HEU5467505.1"
                     /translation="MRIVQSLALLSVVVPAGLMAQGATNAPVAPAAAPVITWPTLPLK
                     YAAKPTHPEITAEDLMSRLYIYADDSLQGREAGQLGNFKATTYIAAEAKRMGLVPAGD
                     SGTYFQTVPYKQRVFDTTSTIMVGGATLKAPAEFLVLAGDAGTISAPVIYGGDLNDEN
                     RADLDSNAVKGKIVLFTAKGGMNFRQARRAPRVKGAVAALIAGLDDFPPQFLGFFSRP
                     RGFLDYHAPGAPAQLPNVFITKAAAAKFFTNPVDQLAVGALGSDATINLKWVVTPMPY
                     PARNVVGIVRGSDPKLRAEYVAVGGHNDHIGMTARPVDHDSMRIFLRVVRPEGAEQNG
                     KPATAAEQAEVDSILAAYRKGHPGTERLDSISNGADDDGSGTVSVLEIAERISSLKVK
                     PKRSIIFVWHVGEEKGLLGSQYFTDFPTVPRDSIVAQLNIDMVGRGDATDETGRACKM
                     DTEKNRCEAGVDFEATSTPLHGNPNYLQIIGSRRLSTELGDLIEKVNTQDKHGLSFDY
                     SEDANGHPTNIYCRSDHYEYARYGIPIAFFTTGGHADYHQVTDEPQEIDYKHMAKIDN
                     LIEDIAIHVADLDHRPVVDHPKPDPFGACQQ"
     gene            3112..4251
                     /locus_tag="VFU75_11490"
     CDS             3112..4251
                     /locus_tag="VFU75_11490"
                     /inference="COORDINATES: protein motif:HMM:NF026257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEU5467506.1"
                     /translation="MRDRVVDALRLRTWAIRVLSAGWQPPPDVPVDAWKLFLKAERCA
                     VALSTRAEGDAPPVLHAVATIELQRILSARAQLEELGRRVHALGERIMVLKGGLMALG
                     SASGVDLMDIDVLTERDSAQRIAGLLDAQGYLVEGPDSPAHLAQRRQPFAVHIEVHHS
                     LAECSAAEVWAHAEPVPGRPGLWKAAALDQVWHALRHTAYTHPFRRGALRDLILIGWA
                     DTGCDQASHEELQGRMGTDEVLRALLRVARDLRDDRAPIDAFRREAAAHYLLSQRPAR
                     FGEFGLRPPVVRSVYSMLDGPEARSAYWNSVWNGRSEISPWSALARVERAWPLGGRLA
                     RRLARLARVPVVEILALPIAHRAAKLARTYARIEPRTLDIRSGEP"
     gene            complement(4200..5120)
                     /locus_tag="VFU75_11495"
     CDS             complement(4200..5120)
                     /locus_tag="VFU75_11495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467507.1"
                     /translation="MALSLTADEALSITVEEPAAPFTARWLPRGLADLSGRARATIDV
                     KVGRAAEAPATMPTLTVGPVRGWMSDRYLILRGATAGGDVDLINCRAVLRVEPSAGDT
                     GEADMYGMLTIAAGVLLAGLGRALIHGAAVVPPGDGAWVLIGDSGSGKSTTCATLCRA
                     GWSYLSDDQVILSAAERGLVAEGWVRPFHLDGGWELGKRWGKREALDAARLFPGLRRV
                     TAPFAGLLLPKVAAQLSTATHPADPAEALAELIRQSPWVLLDRGAAPVVLDLLAAAAS
                     RCRPPQRLTLGGDSYGSPERMSRVLGSILA"
     gene            complement(5122..5379)
                     /locus_tag="VFU75_11500"
     CDS             complement(5122..5379)
                     /locus_tag="VFU75_11500"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HEU5467508.1"
                     /translation="MSASRVAKNVYAAQLAGEAVILHLDTKRYYRLNATGAEVWKGIE
                     AGESREVLVERLVREFTVERDVAAAEVDRLVERLREAHLIE"
     gene            complement(5376..6251)
                     /locus_tag="VFU75_11505"
     CDS             complement(5376..6251)
                     /locus_tag="VFU75_11505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467509.1"
                     /translation="MTRRGIAAVALTLGACSDGGGPTPPPSLTFDLLRGSHRSIYRVR
                     VDGTDTLRLTTDTADDRQPTSAAGTIVFVSSRDGNAELYAMPAAGGTATRLTNTAANE
                     ADPALSPDGTKLAYTRDDGGLPRLWISDANGSGAVRVTDSLDFGGAVDVSPTWSPGSD
                     RVAFVSTTAGSARLYQLTLNGMTIVPLLSDTAVDVEPSWSPDGSRMAFTWGGNGGARI
                     AILDLATHGVSLLTPASAQNGQPAWLLDGRLTFLQEGASPALAWIDPSAPSVVHGIDV
                     GAGTPGHPSAIKPQQ"
     gene            complement(6248..8482)
                     /locus_tag="VFU75_11510"
     CDS             complement(6248..8482)
                     /locus_tag="VFU75_11510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467510.1"
                     /translation="MFRVVRRGFMPAAAIAGVLSCSDRTPLAPVTAVGTSPDALITCT
                     ATVATASIACTKPGGNPHLKSELQTGGQGIYVTLRSTNVSYVGGVDSIFQGDVTVQNL
                     MAQTLGDSNSVVTGVRVFFLTGPDNGVTMVGDSVGTFTVSGQHYFVYNQAINPFGVSS
                     SKTWQWKLHGASTFSFSVAVSAKVEDDAGVLRWAPQTTTSTITGIWGASANAVYAVAQ
                     EGSIWRFDGTTWKDQPSGGFNGLLGIWGSDTNDIYAVGGGGDILHYDGRSWAQETSGT
                     GNGLAGISGTSGSDIYAVGINHTILHFNGTAWSAMTDSSTQDFASVWVATSTAIFATG
                     SNGELWKGDGTTWTKLGGLVSPTNCFSVWASSATDVYAACAGGAILHYDGSTVSTVPS
                     GQSSNLNAVWGSSASDVYVAGDGGILLHSTDNGANWALVPNAFVGNLLSAWGLNSTTI
                     YVGGTQGTVQQYNGTTLSTVLQEHADALNSVWGTSASDAYAVGGNGVVVHYDGTTWTT
                     TATLAASFLNSVWGSASNDVYTAGAASVATKAVVYHSTNGIAWTSDTVVTTGDGNLNA
                     VWGASGTSIYAVGDGGVIIHGQHGSFATQTSNTANDLLGVWGTTDADVWAVGVSGTID
                     HGVNGTWTATTQGTEDINGIWAADASHVFAVTAGGSILTWNGSTWNTLSAVSPTPLIA
                     VWGTSATDVYAAGGSTLLHYNGSAWTVQSLGISTATLDLLSVWGSSSTDLFAVGSGIY
                     HGVR"
     gene            complement(8470..9468)
                     /locus_tag="VFU75_11515"
     CDS             complement(8470..9468)
                     /locus_tag="VFU75_11515"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEU5467511.1"
                     /translation="MLLGFALTQHCNLRCPHCIRDDVTTVQALDVSLIERTLDDARHL
                     FGDVTASFTGGEPLLHPRFAEVIAACAGRGIPYRFVSNGWHLGRVMPLLDRHPPQAVR
                     LSLSGADAQVHDAERGRDSFRRVLLAVTMLTSRQIPVELSIVIDRRDRHQISEVADLA
                     EGLGCLRLHYILPQPVPGSVLRDSDLPPEEWLPVVREINAVARRPGMRTAIQIDYGAP
                     SDEPEVRCDTFALRRVYVDARGRLSTCCQLSEYGFNEADVVADLHSESLQEVWPRYVA
                     RLRAQEEASRPAGGDPLDPFPCLRCAKACGKLDWLRAYPENSWAQALIPLESTCSV"
     gene            complement(9479..9631)
                     /locus_tag="VFU75_11520"
     CDS             complement(9479..9631)
                     /locus_tag="VFU75_11520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467512.1"
                     /translation="MESPQPQDEPTPPGKRPWQAPELTELPKLTDLTLISGIPGGGGI
                     GGSTVF"
     gene            9884..11128
                     /locus_tag="VFU75_11525"
     CDS             9884..11128
                     /locus_tag="VFU75_11525"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HEU5467513.1"
                     /translation="MDIPRAPVKNRKRLIQGGIGVGVLIVLTLIISSLSPAAPPVDRG
                     TLWVDSVRQGPMVRDLRGPGTLVPEHIRYITALSAGRVDKVLAQPSSHVKPGDVLLEL
                     SNPDVQIQALQAEEQLTAARAQLVSLRTTLETQRLAQAGTLATMRTAYAKAQRDVSEA
                     DSLAALHLLSDFEVASRRDIAKEDSTRLDIEEQRLVLMTETVDSQLAVQQSQVDRLHA
                     IADFQQNVVRSLSVRAPDSGVVSDLTLQLGQYVTAGTQLAKVVQPSKLKAVLQIPETQ
                     AKDVVIGQAASIDTRNGIIPGHVIRSDPNAINGTVAVDVALDGTAPGMRPDLSVDGTI
                     VVERLNNVLFTGRPAYGQPNSTIGMFKISDDGKYATRVQVQVGRNSVGSIEIIRGLAV
                     HDSVILSDMSQWDNVDKVRIKR"
     gene            11209..11913
                     /locus_tag="VFU75_11530"
     CDS             11209..11913
                     /locus_tag="VFU75_11530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5467514.1"
                     /translation="MAGNGSSLIQLQGIKKVFYTDEVETHALAEVHLDVLKGEYVAIS
                     GPSGCGKTTLLSILGLLDTPSDGQYILDGRPVANLSAADRARVRNRQIGFIFQAFNLI
                     GDLTVYENVELPLTYRGMSSGDRKQRVQQALEKVGMSHRMKHYPAQLSGGQQQRVAVA
                     RAVAGDPAILLADEPTGNLDSSNGEAVMDLLRELHKGGATICMVTHDPRYARHADRNI
                     ELFDGRVLEEHAGQKA"
     gene            12009..>12283
                     /locus_tag="VFU75_11535"
     CDS             12009..>12283
                     /locus_tag="VFU75_11535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467515.1"
                     /translation="MDSLFQDLRYAARSLARTPVLAVAAVLTLGIGIGANSAMFGVVD
                     RLFFRPPALVKDPSRVRAVDFTVTSRTFGTYTAPLGTYPRLVDFQRRA"
BASE COUNT         2162 a         4149 c         3957 g         2015 t
ORIGIN      
        1 tcctcgtgat cgtgtcggcc tttttcccgc tcgcgccgat cgtggacgcc gtgaccggcg
       61 cggtccccgg tgacgccgcc ctcacccgcc ccgccctgta cgtcatcctg gcgccactct
      121 cgaacacgct ggatgccctc accttcctca ccatcgagcg cgccgaggtc cttctcgcta
      181 cctgggtcat tctcttcatc ttgtggggcg tcgtggccgg acggacccgt cgcggccggt
      241 tgatccgcat cggcctcgcc cccatcgtcg tgtttgcgtt cgccggcgcc gccgtcaccc
      301 tcccccgccc ggtgcccgcc ctggaatcgg cggactccga cgcgaccatc atcgatttcc
      361 actcccacac gcaggcgtcg catgacggcc ggcacgggtg gaccgatgac gacctggccc
      421 gctggcacgc cgatcagggg ttcgaagccg cctacgtcac cgatcacaac gtgatctatc
      481 ccggacggga aaacggggtg atcgccctgc tcccgggcgt cgaatggagc gtgtaccgcc
      541 agcacatcgt cgccctgggc cgggctcagc cgatcgactc gaccagccac gccctgtttt
      601 cgaaagacac gccgggcatg ctgaagctct tcgactacct gcatcagcag ggcgcgctcg
      661 cgatcgcgtc catccccgag tactgggaga atcaccggga ggacctggag cagttcgtga
      721 ccgcgggcgt ggacggcttt gagatcgtga attgcgcccc gaaagcactg cggttctcga
      781 gcgccgaccg ggccgacgtc ctgctcctgg cgaagagcca cgacctgctc gtcacgggcg
      841 ccagcgacaa ccacggatgg gggaaagtca cctgcgtctg gaacctgtcg caggtcggcg
      901 ccaagggctt ccgggccaac gaggtgatcg cacgccccat tgcgctcgcc cagggggacc
      961 ggccgatctg gagcgccgga ttcgaccagc cgtggctgat gctccgcagc ctcacggcgg
     1021 gggagcgcgc cagctggctc acctggatca ccgtttacct catttacatg agcgtaccgc
     1081 gccgtcaggg gaaacgggcc agcatcggca tcctggcgcg ggacctgaca ccacagagag
     1141 attgaaaggc aacttccggg gctgctccgc cgtaggaaca gtgctccttc accccacacc
     1201 caactcggag tatcgatgcg gattgtccaa tcactcgcgc tgctgagcgt cgtcgtcccg
     1261 gccggtctga tggcccaggg agcgacgaac gcgccggtcg cgccggcagc ggccccggtc
     1321 atcacctggc cgaccctgcc cttgaagtac gccgcgaaac cgactcatcc ggaaatcacc
     1381 gccgaagacc tgatgtcccg cctctacatc tacgcggatg attcgctgca gggccgcgaa
     1441 gcgggccagc ttggaaactt caaggcgacc acctacatcg ccgcggaagc caagcgcatg
     1501 ggtctcgtcc ccgcgggcga cagcgggacg tacttccaga ccgtgcccta caagcagcgc
     1561 gtgttcgaca ccacttccac gatcatggtg ggtggtgcga cgctgaaggc ccccgccgaa
     1621 ttcctggtcc tcgccggcga tgccgggacg atctcggcgc ccgtgatcta cgggggcgat
     1681 ctgaacgacg agaaccgcgc ggatctcgac tcgaacgcag tgaagggcaa gatcgtcctg
     1741 ttcaccgcca agggcggcat gaacttccgg caggcacggc gcgcaccccg agtcaaaggt
     1801 gccgtagcgg ccctcatcgc cggactcgac gacttcccgc cgcagttcct cggcttcttc
     1861 agccgtccgc gggggttcct ggattaccac gcgcccggcg ccccggccca gctgcccaac
     1921 gtcttcatca cgaaggcggc ggccgcgaag ttcttcacga atcccgtgga tcagctggcg
     1981 gtgggcgcgc tgggcagcga tgcgacgatc aatctcaagt gggtcgtcac gcccatgccg
     2041 taccccgcac ggaacgtcgt cggcatcgtc cggggctcgg atcccaagct gagggctgag
     2101 tacgtcgccg tcggcggcca caacgatcac atcggcatga ccgcccgccc cgtcgaccac
     2161 gactccatgc gcatcttcct gcgcgtggtc cgtcccgaag gcgccgaaca gaacggcaag
     2221 ccggcgaccg ccgccgagca ggccgaagtg gattcgatcc tcgcggcgta ccggaagggg
     2281 catcccggaa cggagcggct cgattcgatt tccaacggcg ccgatgacga cggctccggc
     2341 acggtgtccg tgctcgagat cgccgagcgc atttcgagcc tcaaggtgaa gccgaagcgg
     2401 tcgatcatct tcgtctggca cgtcggcgaa gaaaagggtc tgctgggctc gcagtacttc
     2461 accgacttcc ccacggtgcc ccgcgattcg atcgtggcgc agctcaatat cgatatggtc
     2521 ggccgcggcg acgcgaccga cgagacggga cgcgcctgca agatggacac cgagaagaac
     2581 cgctgcgagg cgggcgtgga tttcgaggcc accagcacac ccctgcacgg caacccgaac
     2641 tatctgcaga tcatcgggtc ccgccgcctg tcgacggagc tgggcgactt gatcgagaag
     2701 gtcaataccc aggacaagca cggtctgtcg ttcgactact ccgaagacgc caacggccat
     2761 ccgacgaaca tctactgccg ctcggatcac tacgagtacg cccggtacgg catcccgatc
     2821 gcgttcttca cgaccggcgg tcatgccgac tatcaccagg tcacggacga gccgcaggag
     2881 atcgactaca agcacatggc caagatcgac aacctcatcg aagacattgc gattcatgtc
     2941 gccgatctgg atcacaggcc cgttgtcgat catccgaagc cagatccgtt tggtgcctgc
     3001 cagcagtaaa cagacggcta ggcgctcaga cagacgcctg ggcgcctagt cgccaagcgg
     3061 gttgcgggaa caagtggcca ggacaacacc tcctggccac ttttcttttc gatgagagat
     3121 cgcgtggtag acgcgctacg actgcggacg tgggccatcc gggtcctgtc cgccggctgg
     3181 caaccgccac ccgacgttcc ggtcgacgcc tggaagctct tcctcaaggc cgagcggtgc
     3241 gcggtggcgt tgtcgaccag ggccgaaggc gacgcgcccc ccgtcctcca tgccgtcgcg
     3301 accatcgagc tccaacggat tctctcggcg cgtgcccagc tcgaggagtt gggacgccgg
     3361 gtccacgcct tgggcgagcg gatcatggtg ctgaagggcg ggctgatggc cctcggcagc
     3421 gcgtccggtg tcgatctcat ggacatcgac gtcctgaccg agcgagactc cgcacagcgg
     3481 atcgcgggtc tccttgatgc tcaaggctac ttggttgagg gcccggactc gcccgcccac
     3541 ctggcccagc gccgtcaacc gttcgcggtc cacatcgaag tgcaccattc gttggcggag
     3601 tgctccgcgg cggaggtgtg ggcccacgcg gagcccgtac ccggccggcc gggactctgg
     3661 aaggccgccg cgttggacca ggtgtggcac gcccttcggc atacggccta cacccatccc
     3721 ttccggcgcg gagccctgcg ggacctcatc ctcatcggct gggcagacac agggtgcgac
     3781 caggcctcgc atgaagaact ccagggacgg atggggaccg atgaagttct ccgcgccctc
     3841 ctccgggtgg cccgcgacct gcgcgacgac cgggcaccca tcgacgcttt ccggcgggag
     3901 gccgccgcgc actacctgct ctcacagcgc cccgcgcgtt tcggtgaatt cggactccgc
     3961 ccgcctgtgg tccgttcggt gtattccatg ctcgacggtc ctgaggcccg gagtgcctac
     4021 tggaactcgg tatggaacgg caggagtgag atttctccat ggagcgcgct ggcaagggtc
     4081 gagcgcgcct ggccgctcgg ggggaggctc gcacgtcgtt tagcgcgact cgctcgggtt
     4141 ccggtggtcg aaatcttggc cctgcccatc gcgcaccgcg ccgcgaagct ggcccgcacc
     4201 tatgcgagaa tcgagccgag aaccctggac atccgctcgg gcgagccgta ggaatcgccg
     4261 ccgagagtga gccgctgtgg gggacggcac cgtgatgccg ccgcggcgag gaggtcgagc
     4321 acgacgggag cggcaccccg gtccagcagg acccacggcg attgccggat cagctcggcc
     4381 aaggcctcgg cgggatccgc tggatgcgtg gcggtcgata gctgcgccgc gaccttaggt
     4441 aacaacaggc cggcgaaggg tgcagtgact cgccggagcc ccggaaagag acgcgccgca
     4501 tcgagggcct ctcgcttgcc ccatcgcttg cccaactccc atcccccatc gagatgaaaa
     4561 ggccgcaccc agccttcggc caccagtcct cgttccgcgg cggacaggat gacctgatca
     4621 tccgaaaggt atgaccagcc ggctcggcag agcgtagcgc aagtagtcga cttccccgaa
     4681 ccagagtccc caatcaggac ccaggcgccg tcgccaggcg gcaccaccgc cgccccatgg
     4741 atcagcgcac ggcccagtcc ggcgaggagc acgcccgccg cgatggtgag catgccgtac
     4801 atgtcggcct cgcccgtgtc cccggccgac ggctccactc gcagcaccgc gcggcaattg
     4861 atgagatcga cgtcgccccc ggcggtcgcg ccccgcagga tcaggtaccg gtccgacatc
     4921 cagccccgca ccggaccgac ggtgagtgtg ggcatggtcg ctggagcctc tgcggcccgc
     4981 cccaccttca cgtcgatcgt cgcgcgcgct cggccagaca aatccgccag accccgggga
     5041 agccatcgtg ccgtaaacgg cgcggccggc tcctctacgg tgatcgacag cgcttcgtcg
     5101 gctgtgaggg aaagagccat gctactcgat aagatgagct tcgcgcagac gctcgaccaa
     5161 ccggtcgacc tcggccgctg ccacgtcgcg ctcgaccgtg aactcccgta ccaggcgctc
     5221 gaccagcact tcgcgactct cgcccgcttc gatgcccttc cacacctcgg ccccggtcgc
     5281 attcagacgg tagtagcgct tggtgtcgag gtggagaatc accgcctcac cggcgagctg
     5341 ggcggcgtac acgttcttcg cgacgcgaga agcgctcact gctggggctt gatcgcggaa
     5401 ggatgtccgg gcgtgccggc gccgacgtcg atcccgtgca cgaccgaggg tgccgaggga
     5461 tcgatccacg cgagtgcggg ggacgccccc tcctggagga aggtgagccg gccatccagc
     5521 agccaggcgg gctgcccgtt ctgagcagaa gccggcgtca gcagggagac gccgtgcgtt
     5581 gcgagatcca ggatggcgat gcgcgctccg ccatttcccc cccaagtgaa tgccatcctg
     5641 ctcccatcag ggctccagga cggctcgaca tccaccgccg tgtcggacag aagggggaca
     5701 atggtcatcc cgttgagggt caactgatag agccgggccg aacccgcagt cgtggaaacg
     5761 aatgcgaccc ggtcggaccc gggggaccag gtcggcgaga cgtcgacggc tccgccgaag
     5821 tccagtgagt cggtgacgcg gaccgcgccg gaaccgttgg cgtcggaaat ccagagtctg
     5881 gggagaccgc cgtcatcgcg ggtgtaggcg agtttggtcc cgtcgggtga cagggccgga
     5941 tcggcctcgt tcgccgccgt gttggtcaag cgcgtcgccg ttcccccggc ggccggcatg
     6001 gcatagagct cggcattccc gtcccggctc gagacgaaca cgatggtccc ggccgccgac
     6061 gtcggctggc ggtcgtccgc ggtgtcggtg gtgaggcgaa gcgtgtcggt cccgtcaacc
     6121 cgcacgcgat agatgctgcg gtggctcccg cgcaggaggt cgaaggtcag ggacggcgga
     6181 ggggtgggac cgccgccgtc actgcacgcc ccgagagtca gcgcgacggc ggcgatcccg
     6241 cgacgcgtca ccggacgccg tgataaatgc ctgaccccac ggcgaagagg tcggtcgacg
     6301 acgaacccca gacactgagc agatccagcg tcgcggtcga gattccgagc gactgtacgg
     6361 tccaggcgct tccattgtaa tgcaggagcg tgctcccacc cgccgcatag acgtcggtgg
     6421 cggaggtgcc ccagacggcg atcagcggag tcggcgagac agcggagagt gtgttccagg
     6481 tggacccgtt ccaggtgagg atggatccgc ccgcggtcac cgcgaagacg tgactcgcgt
     6541 cggcggccca gatgccgttg atgtcttcgg tcccctgggt agtagcggtc cacgttccgt
     6601 ttacaccatg atcgattgtt ccactcactc caacagccca gacgtcggca tccgtcgtgc
     6661 cccagacgcc gagcaagtcg ttcgcggtgt tgctggtctg tgtggcaaag cttccgtgtt
     6721 gtccgtgaat gatcacgccg ccgtcgccca ctgcatagat cgacgtaccc gaggcccccc
     6781 acaccgcgtt gagattcccg tcacccgtgg tgacgaccgt gtccgacgtc caggcgatgc
     6841 cgttggtgga gtgatagacc acagccttcg tggccacgct ggctgcaccg gccgtgtaga
     6901 cgtcattcga ggcactgccc cagacgctgt tcaggaatga cgcggccagc gtggccgttg
     6961 tggtccaggt cgtcccgtcg tagtggacga cgaccccatt gcctcccacc gcgtaggcat
     7021 cgctggcgct ggtcccccac acggaattga gcgcgtccgc gtgctcctgg aggaccgtgc
     7081 tcaaggtcgt gccgttgtac tgctgcacgg tgccttgcgt accacctacg tagatcgtgg
     7141 tcgaattcag tccccaggcg gagagcaggt tgccgacgaa cgcgttcggg accaaggccc
     7201 agtttgcgcc gttgtcagtc gagtggagaa gtatcccgcc atcgccggcc acgtagacgt
     7261 ccgaggccga cgagccccag accgcattaa ggttgcttga ctggccactg ggaacggtgg
     7321 acacggtgga gccgtcgtag tgcaggattg ctccacccgc gcatgccgca tacacgtccg
     7381 tagcggagct cgcccacacg ctgaagcagt tcgtcgggct caccagccca cccagcttgg
     7441 tccaggtggt cccatccccc ttccacagct cgccgttgct gccggtggcg aaaatcgcag
     7501 tactggtcgc gacccacacc gaggcaaagt cctgcgtgga gctgtcggtc atcgcgctcc
     7561 atgcagtacc gttgaaatgc aggatggtgt ggttgatacc gaccgcgtag atgtcgctgc
     7621 ctgaggttcc tgaaatgccc gcaagtccgt ttccggtacc ggaggtctcc tgcgcccagc
     7681 tgcggccgtc atagtgcagg atgtccccgc cgccgcccac cgcgtagatg tcgttggtat
     7741 cgctgcccca gatccccagg aggccattga agccgccgga gggctggtcc ttccaggtcg
     7801 tgccgtcgaa acgccagatg cttccctctt gagcgacggc gtacaccgcg ttggcgcttg
     7861 caccccagat gccggtgatc gtgctggtcg tagtctgcgg cgcccaccgg agcacgccgg
     7921 cgtcatcctc gaccttggcc gacacagcga cggagaagct gaacgtggaa gctccgtgga
     7981 gcttccactg ccaagtcttg gaagacgaca caccgaatgg attgattgcc tgattataga
     8041 cgaagtagtg ctgaccactg accgtgaacg tgcctaccga gtcaccaacc atggtcacgc
     8101 cgttgtccgg tccggtgagg aaaaacacgc ggactccggt gacaacggag ttactgtcgc
     8161 caagcgtctg ggccatcaga ttctgaaccg tgacgtcgcc ctggaagatc gagtcgacgc
     8221 cgccgacata gctcacgttt gtagaccgca gggtgacgta gatcccctgc ccgcccgtct
     8281 gcagctccga cttgagatgc gggttgccgc caggcttcgt gcaagcgatc gacgcggtcg
     8341 ccaccgttgc cgtgcaagtg atcagggcgt ccgggcttgt ccctacagca gtgaccggcg
     8401 cgagcggcgt ccggtcggag cagctgagga cgccggcgat ggcggcggcg ggcatgaagc
     8461 cccggcggac tacacggaac atgtcgactc cagagggatg agggcctggg cccaggaatt
     8521 ctcagggtac gcacgcagcc agtcgagctt gccgcacgcc ttggcacaac gcagacaggg
     8581 gaacggatcg agaggatcgc cgcccgcagg cctcgaggcc tcttcctgcg cgcggaggcg
     8641 ggcgacatac cgtggccata cctcttgcaa cgactcactg tgcagatcgg cgacgacgtc
     8701 cgcctcgttg aaaccgtact cggacaactg acagcaagtc gacagccgtc cgcgggcgtc
     8761 gacgtacacg cgccggagcg cgaaggtgtc gcaccgaacc tcgggttcgt ccgacggcgc
     8821 accgtagtcg atctgaatcg ccgtccgcat cccgggccgt cgtgcgaccg cgttgatttc
     8881 ccgcaccacg gggagccatt cctcgggcgg aagatccgaa tcccgcagga cgctccccgg
     8941 caccggctgc ggaagtatgt aatgaaggcg gaggcatcca agcccttcag ccagatccgc
     9001 cacctcactg atctgatggc gatcgcggcg atcgatcacg atcgagagct cgacgggaat
     9061 ctggcggctc gtgagcatcg tcacggcgag gagcacccgc cggaaagaat cgcgcccccg
     9121 ctcggcgtca tgcacctgcg catcggcgcc cgagagtgac aggcgaacgg cctgcggcgg
     9181 atggcggtcg agcaggggca tcacgcgccc cagatgccac ccgttggaca cgaaccggta
     9241 cggaatcccc cgtccggcac aggcggcaat gacttcggcg aaccggggat gcagcagcgg
     9301 ctcaccgccc gtgaaactcg cggtgacgtc gccaaagagg tggcgggcgt cgtcgagcgt
     9361 ccgctcgatg agggaaacgt cgagcgcctg caccgtcgtg acatcgtcac ggatgcaatg
     9421 tgggcaccgg agattgcagt gctgagtcag ggcgaagccg agcagcacga tgggcggcct
     9481 agaagacggt gctgccgccg atgccgcccc cgcccgggat tcccgagatc agcgtgagat
     9541 cggtgagctt gggcagctcg gtcaattcag gagcctgcca cggacgcttt ccgggtgggg
     9601 tgggctcgtc ttgtggttgg ggtgattcca cgatcatcct ctttatgtgc gcacaggggt
     9661 tgatagtctg ccggttgcca ggccatccgt gccgtgactg ggaacacggg caaatctacg
     9721 aggggggacc cattccaaat cgtccgaaat tgggactgcg aatcccgcct tcggacggcc
     9781 gcggccaacc attcgcgacc aacgcccgtc taactggcga ctccgtaagg gattaagggg
     9841 atcctccgct ggcacgggcc gtgccttagt ggatctatcg gatatggata tcccacgcgc
     9901 tccagtcaag aaccggaagc ggctgatcca gggtggtatc ggggtcggcg tgctgatcgt
     9961 gttgaccctc atcatttcct cgctctcccc ggccgctcct ccggtggacc ggggaacgct
    10021 gtgggtcgac tccgtgcgcc agggtccgat ggtccgagac ctccgcggtc ccggtaccct
    10081 cgtgcccgag cacatccggt acatcaccgc cctttcagct ggccgcgtcg acaaggtgtt
    10141 ggcgcagccc tcttcgcatg tgaagcccgg ggacgtcctc ctcgagcttt ccaaccccga
    10201 cgtgcaaatc caggccctgc aggccgagga gcagttgact gcggcaaggg cacagttggt
    10261 gagtctgcgc accaccctcg agacacagcg gctggcgcag gctgggaccc tggccacgat
    10321 gcgcaccgca tacgcgaagg cacagcgcga tgtcagcgaa gccgacagcc tggccgctct
    10381 tcatttgctg tcagatttcg aagtggcctc ccggcgggat atcgcgaagg aagattccac
    10441 gcgcctcgac atcgaggagc agcgtctggt gctgatgacc gagaccgtgg actcccagct
    10501 cgccgtgcag caatcacaag tcgaccggct ccacgccatc gctgatttcc agcaaaacgt
    10561 ggtgcgatca ctctccgtcc gggcccccga ttcaggcgtc gtctccgacc tcacgctgca
    10621 gctcggccag tacgtcacgg cggggacgca actggcgaag gtggttcagc catccaagct
    10681 caaggccgtc ctccagatcc ctgaaacgca ggccaaggac gtcgtcatcg ggcaggctgc
    10741 gtccatcgac acccggaacg gcatcatccc ggggcacgtc atacggtcgg acccgaacgc
    10801 gatcaacggg acggtggccg tggacgtggc gctcgacggc acggcccccg gcatgcggcc
    10861 cgacctctcg gtcgacggga ccatcgtcgt cgaacgcctc aacaacgtgc tcttcacggg
    10921 ccgtcccgcg tacggccagc cgaacagcac catcgggatg ttcaagatct ccgatgacgg
    10981 caagtacgcc acgcgggtgc aggtccaagt gggccgaaat tcggtgggca gcatcgagat
    11041 catccggggg ctggcggtac acgactcggt gattctctcg gatatgtccc agtgggataa
    11101 cgtcgataag gtgcggatca agagataggc gtcaggggcc agggatcagg gattggttgg
    11161 ggacttctga tccctgaccc ctgacccctc aaacaaggag atccggaaat ggcggggaac
    11221 ggttcatctc tcattcagct gcaaggcatc aagaaggtct tctacacgga cgaagtggag
    11281 acccacgccc tggcggaggt ccatctcgat gtgctgaagg gggagtacgt cgccatctcg
    11341 ggaccatcgg ggtgcggcaa gaccaccctg ctctcgatcc tcgggctcct cgatacgccc
    11401 tccgatgggc aatacatcct ggacgggcgg cccgtcgcga atctctcggc ggctgaccgc
    11461 gctcgcgtcc gcaaccggca gatcgggttc atcttccagg ccttcaatct catcggcgat
    11521 ctgacggtct acgagaacgt cgagctcccc ctcacgtacc gcggcatgtc gtccggagac
    11581 cgcaagcagc gggtccagca ggcactcgag aaagtcggga tgtcgcaccg gatgaagcat
    11641 tacccggccc agctctccgg cggtcagcag cagcgcgtgg cggtcgcccg cgccgtggcc
    11701 ggtgacccgg cgatcctcct tgccgacgag ccaaccggaa acctcgacag ctcgaacggc
    11761 gaggccgtga tggatctgct ccgcgagctg cacaagggcg gcgccacgat ctgcatggtc
    11821 acgcacgatc cccggtacgc gcggcacgcc gaccgcaaca tcgagctgtt cgacggccgg
    11881 gtgctggagg agcacgcggg ccagaaggcg tagcacggga agcgtgaaac gggacacggg
    11941 aaacgtgggg tcccgttgcc gacgtttccc gtctcacgat tcccgtttcc cgtccgaacg
    12001 gagctttcat ggactcgctc ttccaggatc ttcggtacgc cgcacgttcg ctggcccgga
    12061 cgcccgtcct ggccgtcgcg gccgtgttga cgctcggcat cggcatcggt gccaacagcg
    12121 cgatgttcgg cgtggtggac cgtctcttct tccggccgcc ggcgctggtc aaggacccga
    12181 gccgcgtgcg cgccgtcgat ttcacggtca cgtcgcggac gttcggcacc tacaccgcgc
    12241 cgctcggcac ctatccccgg ctcgtcgact tccagcgacg cgc
//
LOCUS       DASUBP010000302         6053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1347352, whole genome shotgun sequence.
ACCESSION   DASUBP010000302 DASUBP010000000
VERSION     DASUBP010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6053
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1347352"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..477)
                     /locus_tag="VFU75_11540"
     CDS             complement(<1..477)
                     /locus_tag="VFU75_11540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467516.1"
                     /translation="MTKEQTARRLDGWWTRFMGAIVALIALTVQPSSRLAGQVGYDPA
                     HSPYRDAPTGSGPVLFAGYLGGGRGRVPVGISDGNTWGVRYNFEFGSASIGLGAAYGQ
                     TTRRIVDPFVAVKNNTSGLIDADVVLVDATLQMALTGPKTWRGLAPYIGASLGVAAG"
     gene            complement(474..2015)
                     /gene="gpmI"
                     /locus_tag="VFU75_11545"
     CDS             complement(474..2015)
                     /gene="gpmI"
                     /locus_tag="VFU75_11545"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046537 -
                     2,3-bisphosphoglycerate-independent phosphoglycerate
                     mutase activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HEU5467517.1"
                     /translation="MATPNTGRTPVVLIVLDGWGFRPGRDGNAIELADTPVWHQLWDR
                     SPRTLLDASGLAVGLPEGQMGNSEVGHLNLGAGRVVPQDIVRISQSIASGEFFKIPEL
                     VTLCEAVRKRGGTLHIMGLLGTGGVHALDTHLLAGVELGHRAGLPVAVHAWTDGRDTG
                     PQSALGFMEKLVEDLKPYRGRAVVSDLVGRYYAMDRDKRWDRIKLAYDAMVKGVGAPS
                     PDPIAAIKAAYAAGETDEFIKPRVIAGTPRIKAGDGIFCFNYRADRMRQICRALAIPG
                     FDGFDVGDRPAVDLVTMTQYDETFPFPMAYGPMVLRNIVAQVLADHGKTMFRTAETEK
                     YAHVTYFFNGGVETPFKNEERLLVPSQKVATYDLAPEMSAVGVTDVLCAAIKKGHHDF
                     ILCNYANGDMVGHTGVLPAGLKAVETVDRCLARVLATAETAGATLLITADHGNCEMMI
                     DPGTGGPHTAHTTNPVPLVIVGDGDLRGELRPGGSLRDVGPTILNLLGVERPADMTGK
                     DLREK"
     gene            2131..3087
                     /locus_tag="VFU75_11550"
     CDS             2131..3087
                     /locus_tag="VFU75_11550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467518.1"
                     /translation="MNTAGAWIRDSAGGKRLAQLAKGTLVDGGRALGTWLPVTIEGWI
                     WGKSVGPGAQGQNDLVVISPQGENLRDRAKGQVVAQLPHGFALQRIDSGRAWIHVRRT
                     GWVSAALMSVVGQVVSTSSTATAAPDSGARPATALPPAPPPAPLDTSGFSTAVPLQHA
                     DLFGAPGGTVTGSIAAPTPLKVLAHSGEWTRVQLEAWVKTTDLASAPIGVLEGVSAAE
                     LRADPAKYAGQVVRWRLQFVAIETADDLRPDIPDGATYLLTRGPAPERGFVYVIVPES
                     KKASVAALTPLTDLEITATVRVGKSHFIGNPIVDLLSFEAVR"
     gene            3084..3446
                     /locus_tag="VFU75_11555"
     CDS             3084..3446
                     /locus_tag="VFU75_11555"
                     /inference="COORDINATES: protein motif:HMM:NF021558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2281 domain-containing protein"
                     /protein_id="HEU5467519.1"
                     /translation="MNDILKERLLRKLETLPEEKAYQVLDYIEFLESKYAERPAGAPA
                     FQKVAETLEDTMRAGRVPITVIKGTMDAVGKAGRFLDGLAAAGRAAVNEVRRNPDKKE
                     IEKKAEPEPPVEDGGTPK"
     gene            3455..4912
                     /gene="guaB"
                     /locus_tag="VFU75_11560"
     CDS             3455..4912
                     /gene="guaB"
                     /locus_tag="VFU75_11560"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEU5467520.1"
                     /translation="MGTFRPTTALTFDDVLLAPRHSTVHPRNVDTKTRFTRSISLNIP
                     LVAAAMDTVTESAMAIAMARAGGIGVIHKNMAIGRQAAEVDRVKRSESGMILNPITLG
                     PDRPLREALKMMERFRISGVPIVDSAGKLVGIITNRDLQFERQLDRPLRDAMTKDNLV
                     TAPLGTTLDEAERLLGKHRIEKLPVVDKDGVLKGLITIKDIFKRREHPDANKDQHGRL
                     RVAAAVGGTPEAKDRAKALLDAGCDVIVIDSAHGHSQGVLETVKLLRESFPDAQLVAG
                     NVATAAGAKALVERGVDAVKVGVGPGSICTTRVVTGVGVPQITAILDALSGTGDVPVI
                     ADGGVKYSGDIVKALAAGASSVMMGSMLAGTEESPGESFLLEGRRFKVIRGMGSLSAM
                     EEGSADRYFQEGEVSPTKLVPEGIEGRVPYKGPVSDVIFQMVGGLRSGMGYCGVCDIQ
                     ALRTETEFLTITSAGLRESHPHDVVITREAPNYSL"
     gene            5005..>6053
                     /locus_tag="VFU75_11565"
     CDS             5005..>6053
                     /locus_tag="VFU75_11565"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEU5467521.1"
                     /translation="MDLFRRKSVAALQAEAETDHSLKRALGALNLTALGIGAIIGTGI
                     FVLTGTVAAVNAGPAVVLSFTIAGVASIFAALCYSEFASLVPMAGSAYTYGYATLGEL
                     FAWIIGWDLVLEYALGAVTVAIGWSGYVVSFLKDIGIVVPAEFSAATGTDVVTASGAH
                     VTAIFNLPAIIIIALVTWLLVVGIKESANFNNIIVLVKVAVVLLFIVFAVKAIHPANW
                     HPFMPPATDNTGQHFGWSGVMAGAGVVFFAYIGFDAVSTAAQEAKNPQRDMPIGIIAS
                     LLICTVLYIIVSGIATGVMSYTELNVPDPIAVVADRAGLGWMSTLIKLGAIAGLSSVI
                     LVMLLGQSRVFWSMS"
BASE COUNT          980 a         1949 c         2101 g         1023 t
ORIGIN      
        1 gcccgccgcg acgccgaggg aagcgccgat gtagggggcg aggcctcgcc acgtcttggg
       61 tccggtgagc gccatttgca gcgtggcgtc cacgagcacc acgtcggcgt cgatgagccc
      121 cgacgtgttg ttcttgacgg cgacgaaggg atcgacgatc cggcgcgtcg tctgtccgta
      181 cgcggcgccc aggccgatcg aggcgctccc gaattcgaag ttgtaacgga ccccccaggt
      241 gttgccgtcc gaaatgccga cgggcacgcg gccccggcca ccgccgaggt agccggcgaa
      301 gaggaccgga cccgatcccg tgggggcgtc gcggtagggc gagtgcgcgg gatcgtagcc
      361 gacttgaccg gctagacggc tagacggttg gacggttagc gcgatcaggg cgacgatggc
      421 ccccatgaac cgggtccacc aaccgtctaa ccgtctagcc gtctgttctt tcgtcatttc
      481 tccctcaagt ctttgccggt catgtccgcc gggcgctcga cgcccagcag gttgagaatc
      541 gtggggccga cgtcccgcag cgatccgccg gggcgcagct ctccgcgcag atcgccgtcc
      601 cccacgatga cgaggggcac ggggttggtc gtgtgcgcgg tatgaggccc gccggtgccc
      661 gggtcgatca tcatctcaca gttgccatgg tccgcggtga tcaagagcgt ggcgccggcc
      721 gtctcggcgg tggcgagcac gcgggccagg cagcggtcga cggtttccac cgctttcagg
      781 ccggccggta ataccccggt atgccctacc atgtccccgt tggcgtagtt gcagaggatg
      841 aagtcgtggt ggcccttctt gatggcggcg cacagaacgt cagtcactcc gacggcgctc
      901 atctccggcg ccaggtcgta cgtggccacc ttctgggaag gcaccagcag ccgctcctca
      961 ttcttgaaag gggtctccac gccgccgttg aagaagtacg tcacgtgggc gtacttctcg
     1021 gtctcggcgg tgcggaacat ggtcttgcca tggtccgcca ggacctgggc cacgatgttc
     1081 cgcagcacca tgggcccgta ggccatcggg aaggggaagg tctcgtcgta ctgcgtcatg
     1141 gtgacgaggt ctaccgcggg gcggtccccg acgtcgaaac catcgaagcc gggaatggcc
     1201 agcgcgcggc agatctggcg catccggtcc gcgcggtagt tgaaacagaa gatcccgtcg
     1261 cccgccttga tgcgcggcgt ccccgcaatc acgcggggct tgatgaactc gtccgtttcg
     1321 ccggccgcat aggcggcctt gatggcggca atcgggtcgg gtgacggggc cccgacgccc
     1381 ttcaccatgg cgtcgtacgc gagcttgatc cggtcccacc gcttgtcgcg gtccatggcg
     1441 taatagcggc ccaccagatc cgacaccacg gcccgcccgc gatacggttt caggtcctcc
     1501 accagctttt ccatgaaccc gagcgcggat tgcggaccgg tgtcgcggcc gtcggtccag
     1561 gcgtgcaccg ccacgggcaa tcccgcgcgg tgaccgagct cgacgccggc gagcaagtgc
     1621 gtgtcgagcg cgtgcacgcc gccggtgccc agcaggccca tgatgtggag ggtgccaccg
     1681 cgtttccgga ccgcttcgca cagggtgacg agctcgggga tcttgaagaa ttcgccggag
     1741 gcgatgctct gcgagatccg gacgatgtcc tgcgggacga cacggccggc accgaggttg
     1801 aggtggccga cctcactgtt gcccatctgt ccttcgggga gccccacggc cagacccgac
     1861 gcgtcgagga gggtgcgggg cgagcggtcc cagagctggt gccagacggg ggtgtcggcg
     1921 agctcgatgg cgttgccgtc ccggccgggg cgaaaacccc agccgtcgag cacgatcagg
     1981 acgaccgggg tgcggccggt gtttggtgtc gccatattca gggaaatgca atctagtcgc
     2041 ggccgaagag ccctaccagt tgcactagcc ctcgcccttg cggcgtctgc gggcgtacgg
     2101 gcccccctcg ccgcccagac tcgctatcag gtgaataccg ccggggcgtg gatccgcgac
     2161 tcggccggtg gcaagcgcct cgcccagctc gccaagggaa cgctggtaga tggcgggagg
     2221 gcgttgggca cgtggctgcc cgtcaccatc gagggttgga tctgggggaa gtcggtgggc
     2281 ccgggggcac agggccagaa cgatctcgtc gtgatcagtc ctcagggcga gaacctccgg
     2341 gaccgcgcga agggacaggt ggtggcgcag ctgccgcacg ggttcgcgct ccagcgcatc
     2401 gactcggggc gcgcctggat tcatgtgcgg cgtaccggct gggtgtcggc cgcgctgatg
     2461 tcggtggtcg gccaagtagt gagcacttca agtaccgcca ccgccgcccc ggacagcggg
     2521 gctaggcctg cgaccgcgct tccgcccgct cctccaccgg cgcctctcga cacctccggt
     2581 ttttcgacgg ccgtcccgtt gcagcatgcc gacctttttg gcgcgccggg agggaccgtc
     2641 acgggatcga tcgcggcacc gacgcccctg aaagtcctgg cgcactcggg agagtggacc
     2701 cgggtgcagc tcgaggcatg ggtcaagacc accgatctcg cgtcggcccc gattggcgta
     2761 ctcgaggggg tgagtgccgc cgagttgcgc gccgaccctg cgaaatacgc cggacaggtg
     2821 gtgcggtggc gcctgcagtt cgtcgcgatc gagaccgccg acgatcttcg tcccgacatc
     2881 cccgacggcg cgacctatct cctgacccgc ggcccggccc cggaacgcgg gttcgtgtat
     2941 gtgatcgttc ccgagagcaa gaaggcgtcg gtggcggccc tcacgccgct cacggatttg
     3001 gagatcacgg cgacggtgcg cgtggggaag agtcacttca tcgggaatcc gatcgtggac
     3061 ctgctctcgt tcgaggcggt gcgatgaacg acattctgaa ggagcggctg ctccggaagc
     3121 tggagacgct gccggaagag aaggcgtatc aggtgctcga ttacatcgag ttcctggagt
     3181 cgaagtacgc ggagcgcccc gccggcgcgc cggcttttca gaaggtcgcg gaaaccctcg
     3241 aggacacgat gcgcgcgggc cgggtaccga tcaccgtcat caagggcacg atggacgccg
     3301 tcgggaaggc gggccggttc ctggacgggc tcgcggcggc gggacgggcg gcagtcaacg
     3361 aagtgcgccg gaatcccgat aagaaggaaa tagagaagaa agcggagccg gagccacccg
     3421 ttgaggatgg ggggacgccg aagtaacttt caagatggga accttccgac cgaccaccgc
     3481 cctcaccttc gacgacgtcc tcctcgcgcc gcggcattcc acggtgcatc cccggaacgt
     3541 cgatacgaaa acccgcttta cccgctcgat ctccctcaac atcccgctcg ttgccgccgc
     3601 catggacacc gtcaccgaat cggcgatggc catcgcgatg gcgcgtgccg gcgggatcgg
     3661 ggtgatccac aagaacatgg ccataggccg ccaggcggcc gaagtggacc gggtgaagcg
     3721 gtccgagtcg gggatgatcc tgaatcccat caccctcggc cccgaccggc ccctgcgcga
     3781 agccctgaag atgatggaac gcttccggat ttccggcgtc cccatcgtgg attcggcggg
     3841 caagctggtc ggcatcatca ccaaccgcga tctccagttc gaacgtcagc tcgaccgtcc
     3901 tctccgggac gcgatgacca aggacaacct ggtcaccgcg ccgctgggca ctacgctgga
     3961 cgaggcggaa cggctcctcg gcaagcaccg gatcgagaag ctcccggtgg tggacaagga
     4021 cggcgtgctc aagggcctga tcaccatcaa ggacatcttc aagcgccgag agcacccgga
     4081 cgccaacaag gaccagcatg gccggctgcg cgtcgccgcc gccgtgggtg ggacgcccga
     4141 ggcgaaggac cgcgccaagg ccctgctcga cgcggggtgc gacgtgatcg tgatcgactc
     4201 ggcgcacggc cactcgcagg gagtgctcga gacggtgaag ctgctgcgtg agtcgttccc
     4261 ggatgcgcag ctcgtcgccg ggaacgtcgc gacggccgcg ggggcaaagg cgctcgtcga
     4321 acggggcgtt gatgccgtga aggtcggtgt cggacccgga tcgatctgca cgacgcgggt
     4381 cgtcaccggt gtgggcgtgc cgcagatcac cgccatcctc gacgccctgt ccgggacggg
     4441 ggacgtcccg gtgattgcgg acggcggcgt gaagtactcg ggggatatcg tgaaggcgct
     4501 cgcggccggt gcgtccagtg tgatgatggg ctcgatgctc gcgggcaccg aagagagtcc
     4561 cggtgagtcg ttcctgctcg aaggccgccg tttcaaggtt atccgcggga tgggctcgct
     4621 ttcggccatg gaagagggtt ccgccgaccg gtacttccag gaaggtgagg tgtcgcccac
     4681 caagctggtg ccggagggca tcgagggccg ggtgccgtac aagggcccgg tctcggacgt
     4741 gatcttccag atggtcgggg gactgcggag cggaatgggg tactgcgggg tgtgcgacat
     4801 ccaggcgctc aggaccgaga ccgagttcct gacgatcacg tcggcgggac tgcgcgaatc
     4861 gcacccgcat gacgtcgtca tcacccgcga agcaccaaac tacagcctct aatcggcttt
     4921 ccctgcttga cgccccgggc gcccggtcct attctggcac ccggcttgcc gacgtttcgt
     4981 ctgtcccatc gctaacgagg gatcatggac ctgttccgac ggaaaagtgt cgcggctttg
     5041 caggcggagg ccgagaccga tcattcgttg aagcgcgccc tgggggcgct gaatctgacc
     5101 gccctcggta tcggcgcgat catcggtacc ggcatcttcg tgctcaccgg tacggtggcg
     5161 gcggtgaacg ccggccccgc ggtggtcctg tcgttcacga tcgcgggtgt ggcgtcgatc
     5221 ttcgcggcgc tctgctactc cgagtttgcg tcgctcgtgc cgatggccgg cagcgcgtac
     5281 acctacgggt acgccaccct cggtgagctc ttcgcctgga tcatcggctg ggacctggtg
     5341 ctggagtacg cgctgggggc ggtgacggtg gcgatcgggt ggtcagggta cgtggtcagc
     5401 ttcctcaagg atatcgggat cgtcgtgccg gcggagtttt cggcggcgac gggcaccgac
     5461 gtggtgacgg cgagcggcgc ccacgtcacc gcgatcttca atctccccgc catcatcatc
     5521 atcgcgctgg tcacctggct cctggtggtc ggcatcaagg aatcggccaa cttcaacaac
     5581 atcatcgtgc tcgtgaaggt ggcggtggtg ctgctcttca tcgtcttcgc ggtgaaggcc
     5641 atccatccgg cgaactggca tcccttcatg ccgccggcga ccgacaacac cggccagcac
     5701 ttcggctggt cgggcgtgat ggcgggggcc ggagtggtgt tcttcgccta catcggattc
     5761 gacgccgtca gtaccgcggc gcaggaagcg aagaaccccc agcgcgacat gcccatcggc
     5821 atcatcgcgt cactgctcat ctgcaccgtg ctctacatca tcgtgtccgg cattgcgacg
     5881 ggcgtgatga gctacaccga gctgaacgtc cccgacccga tcgcggtcgt ggccgaccgg
     5941 gcggggttgg gctggatgtc cacgctgatc aagcttggcg ccatcgccgg attgtcgtcc
     6001 gtcattctcg tcatgctcct cgggcagtcg cgcgtgttct ggagcatgtc gaa
//
LOCUS       DASUBP010000303         2280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1157208, whole genome shotgun sequence.
ACCESSION   DASUBP010000303 DASUBP010000000
VERSION     DASUBP010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1157208"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VFU75_11570"
     CDS             <1..204
                     /locus_tag="VFU75_11570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467522.1"
                     /translation="RRFVRRRLRFVDAVQNPVAPIIAGLVAGAIFLPVTILPLVTVLT
                     AAAFGIGVGTGVASGRKALPPGS"
     gene            289..729
                     /locus_tag="VFU75_11575"
     CDS             289..729
                     /locus_tag="VFU75_11575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467523.1"
                     /translation="MLRATAVVVLSTALAAPVITAQSRWEDQVNDQIKRAAHILVDSL
                     GYTRTHDPYTGSLREGESEYLTLTLHSGTTYRVLGVCDNDCSDIDFRLYDADDNEVDS
                     DTETDDVPIVKVAPRETMRYRLKVIMVACKTSPCFYGVGVFGKE"
     gene            complement(736..1017)
                     /locus_tag="VFU75_11580"
     CDS             complement(736..1017)
                     /locus_tag="VFU75_11580"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU5467524.1"
                     /translation="MFFVVLLHYTKPLSDVDQIRAEHVAHVQNAAGRGIMIAWARRSP
                     PVGGVLVATAPDRKTLEAVLAEDPYVKAGVASPEVVEFNPANLRGPFAS"
     gene            1256..2014
                     /locus_tag="VFU75_11585"
     CDS             1256..2014
                     /locus_tag="VFU75_11585"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5467525.1"
                     /translation="MGASPDADVPPELLADYRRAVARQVEAIEACADRLEQHPTDRDA
                     LETFRREVHKVRGSAGSYGFPEASELAAGMEETAKDWVEHASDVHMDRGALARWFMER
                     ITDFIKPTPSDSTGPLPVRGPSGEGESPEVISIEDDHSLAELLAYGLKSRGYRFLIFR
                     NGAEALEALLKMKIGDRRPLVLLDVDLPGLDGFSLFERLEAERPGVYRVVFTSVHSTE
                     DEQVRALEAGALDYLVKPISLRVVLEKVRRWVGR"
     gene            2011..>2280
                     /locus_tag="VFU75_11590"
     CDS             2011..>2280
                     /locus_tag="VFU75_11590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467526.1"
                     /translation="MTARHLVLIAAVVQGLFVAALLLLIVINRWVRLRRRDAVRPRRE
                     SAEEQFRRWAAGDADVEAVASALVPLPSAIAVERLAAWSSLVPTDR"
BASE COUNT          389 a          750 c          764 g          377 t
ORIGIN      
        1 cgtcggtttg tacggcgccg gctgcggttc gtggacgcgg tgcagaaccc cgtggcgccc
       61 atcatcgccg gcctcgttgc gggcgccatc tttcttccgg tcacgatcct gccactggtg
      121 actgtactga cggccgcagc cttcgggatc ggcgtcggaa ccggcgtcgc ctcggggcgg
      181 aaagctcttc caccgggatc ttgaagaacg actgactttc gggatgggag cgcgggctgc
      241 agattgggac gctcccacgc accccaccca ttcccgagag gtctcgcaat gcttcgagcg
      301 accgccgttg tcgttctgag taccgccctc gccgccccgg tcatcacggc tcagagtcga
      361 tgggaagacc aggtcaacga tcagatcaag cgcgccgccc acatcctcgt ggacagcctc
      421 gggtacacgc ggacgcatga cccctatacg ggatcactgc gcgagggtga gagcgaatac
      481 ctgaccctca cgctgcactc cgggaccacg taccgcgtgc tcggcgtgtg cgacaatgac
      541 tgcagcgaca tcgacttccg gctctacgat gccgacgaca atgaggtgga ctccgacacc
      601 gagaccgacg acgtacccat cgtgaaagtc gctccgcgcg agacgatgcg gtaccgtctg
      661 aaggtcatca tggtggcgtg caagacgtca ccctgctttt acggcgtggg agtcttcggc
      721 aaggagtgag gtcgcctagg acgcgaaggg tccccggagg ttggcgggat tgaactccac
      781 cacttccggg gacgccaccc ccgccttcac gtagggatcc tccgccagca ccgcctcgag
      841 cgtcttccgg tcaggcgccg tcgccaccag caccccgccc acgggggggg accgccgcgc
      901 ccaggctatc atgattcccc ggccggcggc gttctgtaca tgggcgacat gctcggcccg
      961 aatctgatcc acgtccgaca acggcttggt gtagtgcagc aggacgacga agaacatcgt
     1021 gacctcatag cccgggcagc tcttgaccgg cagtgggggg tagcgggtga accgggggtc
     1081 ggacccagcc cgcccgcacc ggtagaaggt cgaaccccgc cggttggtcg acggcagagc
     1141 ccagcggcag gtaagacaga ggccaaccga ctccaggacc gacattaagg gaatatcgga
     1201 ggcagcgccg ctttgtcgct atggcgcgtc gccccatcct ggtcagattc aaggtatggg
     1261 cgcctccccc gatgccgacg tcccccccga gctcctggcc gactaccggc gtgccgtcgc
     1321 cagacaggtc gaggccatcg aggcgtgcgc cgatcgtctg gaacagcatc ccaccgatcg
     1381 cgacgccctt gagacgttcc ggcgcgaagt gcacaaggtc cgtggctcgg cggggtccta
     1441 tggatttccg gaggcttcag agctcgccgc gggcatggaa gagacggcga aggactgggt
     1501 cgagcacgcg agtgacgtcc acatggaccg gggcgcgctc gcacgctggt tcatggaacg
     1561 gatcaccgac ttcatcaagc cgacgccctc cgattccacg gggccgttgc cggtacgggg
     1621 gccgtccggc gaaggcgaat cccccgaggt gatcagcatc gaggacgatc actcactcgc
     1681 ggagttgctg gcgtacggac tcaagagccg cggataccgc ttcctcattt tccgcaacgg
     1741 agccgaagca ctcgaagccc tgctcaagat gaagatcgga gaccggcggc cactggtgct
     1801 gctcgatgtg gacctgccgg gcctcgatgg cttctccctc ttcgagcggc tggaggcgga
     1861 gcggcccggc gtgtatcgcg tggtgttcac gagtgtgcac agcaccgagg acgagcaggt
     1921 gcgggcactg gaagctggcg cactcgacta cctggtcaag ccgatcagtt tgcgggtggt
     1981 gctggagaag gtccgccggt gggtggggag gtgacggccc ggcacctcgt gctcatagcg
     2041 gcagtcgtac aaggtctctt cgtcgccgcg ttgctactgc tcatcgtcat caaccgatgg
     2101 gtgcggctcc gccggcgcga cgcggtccgg ccgcgtcggg agtcggcgga agagcagttc
     2161 cgccgttggg cggccgggga tgcggacgtc gaagcggtcg cgagcgcctt ggtgcccctg
     2221 ccctccgcta tcgccgtcga acgcctcgcc gcctggagct cgctggtgcc gacggaccgc
//
LOCUS       DASUBP010000304         3585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_639897, whole genome shotgun sequence.
ACCESSION   DASUBP010000304 DASUBP010000000
VERSION     DASUBP010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639897"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..541
                     /locus_tag="VFU75_11595"
     CDS             <1..541
                     /locus_tag="VFU75_11595"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HEU5467527.1"
                     /translation="DRALGDTAKRILSEIDRLDAIARGFARFGAPGAEAVALEPVNLL
                     AVARDVVSLYELGEQAGAVTVEGPAGGETVRARKDEIREVLLNMLENSRDAGATRIQI
                     LIDEGGRRLSVRDNGRGIPEEVLPRVFEPTFSTTSSGSGLGLAIAKRLVEGWGATIGI
                     ESRPGSGTTLVVTFPGGRK"
     gene            622..1959
                     /locus_tag="VFU75_11600"
     CDS             622..1959
                     /locus_tag="VFU75_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467528.1"
                     /translation="MRPTLVGVCIVVAIAALPSVAPAQLLSLDARKVGLGGLDLHRGG
                     ALSRYNAAYRAVPDRADPWHAKLTIPIPLGIIQVLKDSTVLHPDSPYFNPLQAANYLL
                     NLPIFLEVKKVPAPTNDIAFGIGKDSFSIDLGKAKSSVPVDPFGFGGSSRPLDLGFGI
                     KGVRASVMMWIQDEVDFQLGPRLIGFLHDGQPAQTNTLYDATGTALAAGGFAPSLGWA
                     GRISGQDQNGVFVGVTGHYYLGLAYGQATSDGGFITGDTLFGGTSPVTPSLGMTTQYS
                     KLGHKMGTGVGADLGVVWVTGPLEAGFGINDVGAKLTWADTRIDTLYFDSSAAVNSTV
                     SRNLQNHVQTTTKLPVTYLANAMLDMGGGTVVGGEILHGARGTEVHMGVEQHFGPLAL
                     RGGIARDQQKKVQFGWGGGVRFLFLSLDVGFATHSTPLSDQRGVTMATSISIY"
     gene            1971..2528
                     /locus_tag="VFU75_11605"
     CDS             1971..2528
                     /locus_tag="VFU75_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467529.1"
                     /translation="MRTMRLSFSVLAAAVLAAACGEGHAIFDVDVYSFIAGTGGDTLH
                     YTTPGIIGTTPPLNNPPLRVTLLPGLGSSVVDTVQVTGAAAIQNNTGTGSLSFKIFFG
                     ADSNTVYSTTPILTTAGNAGPGADTTIMALNATLTSAQDSLFTQKQVFVGVQAVLSNN
                     TTTPLDGRLKLTALHLRLVIKDKVF"
     gene            2618..>3585
                     /locus_tag="VFU75_11610"
     CDS             2618..>3585
                     /locus_tag="VFU75_11610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467530.1"
                     /translation="MRPRSPVLTRRTLTAIRLMAAFSISLLGITCRDNPEAPRGGGRA
                     SFAVRPIIKQHVDLSSFGLVIDSLRLVVVHAPADTLKDTTAYFNPDSTQLHLNLSLIL
                     TAPAESLKVSAILSAGGVPIYSGTQDAIVSVGSSSSGTPIAIPMTFSGPGAGVTALHI
                     APLDSVLHFSDSLRFRVTADSAGIPVTNFYAAWRTSDTLAARINAFGILHAPAARKAI
                     YIIVRTPSGAADSTPITFTPTPTALAIQAGNNQTATVGGLLPAPLQVRVTAADGLGVK
                     GVPVQFTILTGGGSIASAPVVTDTGGYASVTPTLGTLAGAATYQASA"
BASE COUNT          599 a         1185 c         1154 g          647 t
ORIGIN      
        1 cgaccgggcc ctgggagata cggccaagcg gattctctcg gagatcgacc gcctcgatgc
       61 gatcgcccgc ggctttgccc gcttcggtgc gccgggagct gaagcggtgg ccctcgagcc
      121 ggtgaacctg ttggcggtgg cccgggacgt cgtttccctt tacgagctcg gcgagcaggc
      181 cggggctgtt acggtggagg gtccggcggg cggggaaaca gtgcgggccc gaaaggacga
      241 gatccgggag gtgcttttga acatgctcga aaactcccgt gacgccgggg cgacccggat
      301 tcagatcctg atagatgaag gtgggaggcg tctctccgtg cgggacaatg gtcggggaat
      361 tcctgaggag gtccttccac gggtattcga gcccaccttc tccacgacgt cttccggttc
      421 cgggttggga ctggcgatag ccaagaggct ggtggagggg tggggggcta ctattgggat
      481 cgaaagccgt cccggatccg ggacaaccct ggtcgtgacc ttccctggtg ggaggaagtg
      541 accggcctca cccctgcctg tagcaaaaaa tggcatcatt tcccaccttg taggaccaat
      601 cgcccttagc cgaggtgccc tgtgcgcccc acgctggtcg gtgtttgcat cgtggttgcc
      661 attgctgcgc tccctagcgt agcgcccgcg caactccttt cactggatgc gcggaaggtc
      721 ggcctcggag ggctggacct gcatcgtggc ggcgcgttga gccgctacaa cgcggcgtac
      781 cgggcggtgc cggaccgggc ggacccgtgg cacgccaagc ttaccattcc gattccactt
      841 ggaatcattc aagtgctcaa ggacagtacg gtcctgcacc ccgacagccc gtacttcaat
      901 ccgctgcaag cggcgaacta cctcctcaac cttccgatct ttctggaggt gaagaaggtg
      961 ccggcgccga cgaacgacat cgcgttcggg atcgggaagg actcgttcag catcgacctc
     1021 ggaaaggcca agagctcggt gccggtcgat ccctttggat tcggcggatc gagcaggccc
     1081 ctggacctcg gattcggtat caaaggggtc cgggcgagcg tgatgatgtg gatccaggat
     1141 gaggtggatt tccagcttgg tccccggttg atcggcttcc tccacgacgg gcagccggcg
     1201 cagaccaaca cactgtacga cgcgacggga accgcactcg cggcgggcgg gttcgccccc
     1261 tcgcttgggt gggccgggcg catcagcggc caggaccaga acggcgtgtt cgtcggcgtg
     1321 acgggtcact actacctcgg gctggcgtac ggacaggcga cgtccgatgg cgggttcatc
     1381 accggtgaca cgctcttcgg cgggaccagc cccgtcacgc catcgctcgg catgaccacc
     1441 cagtattcga aactcgggca caagatgggg acgggtgtcg gggctgacct gggtgtcgtc
     1501 tgggtgaccg ggcccctcga ggcgggattc ggaatcaacg acgtcggcgc gaagctcacg
     1561 tgggctgata cgcgcattga cacgctgtac ttcgattcgt cagccgcggt caacagcacg
     1621 gtttcgagaa acctccagaa ccacgtgcag accacgacca agctgccggt cacgtacctt
     1681 gccaacgcga tgctggacat gggcgggggc acggtcgtgg gcggcgagat tctccatggt
     1741 gcccgcggca ccgaggtgca catgggggtc gagcagcact tcgggcccct cgcgctccgg
     1801 ggcgggatcg cgcgagatca gcagaagaag gtgcaattcg gatggggtgg gggggtgcgc
     1861 ttcctcttcc tctcgctgga cgtgggattc gccacgcaca gcacaccatt gtctgatcaa
     1921 cggggggtca cgatggcgac ctccatttcc atctactagg cggaggaacc atgcgcacga
     1981 tgcgactgag cttctcggtc ctcgcggcgg cggttctggc cgcggcgtgt ggcgaaggcc
     2041 acgcgatttt cgatgtcgac gtctacagct tcatcgcggg gacgggggga gatacgttgc
     2101 actacaccac ccccgggatc atcggcacga cgccgccctt gaacaatccg cccctgcgcg
     2161 tgaccctgct ccccggtttg ggatcgtcgg tggtggacac cgtccaggtg acgggggccg
     2221 ccgcgattca gaacaacacc ggcacgggat cgctgtcgtt caagatcttc ttcggcgccg
     2281 actcgaatac ggtgtattcg accacgccca ttttgacgac ggcggggaac gcggggccgg
     2341 gcgccgacac caccatcatg gcgctgaacg cgaccctcac gagcgcccag gacagcctgt
     2401 tcacccagaa gcaggtcttc gtcggggtcc aggcggtgct ctcgaacaac accacgaccc
     2461 ctctggacgg caggctcaag ctcaccgccc tccatctccg gctcgtgatc aaggacaagg
     2521 tgttctaggg attccccccg tgtgatcccc gtcactccgg cgggggtcgc acgtcctagc
     2581 tggttgaaac gccgtccccc acattactcg acctccgatg aggccacggt cgcccgtcct
     2641 cacccgccgg accctcaccg ccatccgcct gatggcggct ttttccattt cactcctggg
     2701 gatcacctgc cgggacaacc ctgaagcgcc gcggggcggg ggacgcgcct cctttgccgt
     2761 gcgtcccatc atcaagcagc acgtcgatct ctcgagtttt ggcctcgtta tcgactcctt
     2821 gcggctcgtg gtggtgcacg caccggccga tacgctcaaa gacaccaccg cgtacttcaa
     2881 tcccgactcg actcagctcc acctcaatct ttcgctgatt cttaccgcgc ctgccgaaag
     2941 cctgaaagtt tcggcgattc tgtcggcggg cggggtgccg atctattccg gcacccagga
     3001 cgccatcgtc tcggtcggat cgtcctcgag cggcacgccg atcgcgatcc cgatgacctt
     3061 ctcgggtccg ggcgcgggcg tgaccgctct gcacatcgcc ccgctggact cggtgctcca
     3121 cttctccgac agcctccgct tccgcgtcac tgcggattcc gcgggcatcc cggtcacgaa
     3181 tttctacgcg gcctggcgga ccagcgacac gctcgccgcc agaatcaacg cgttcggcat
     3241 tctccacgct cccgcggccc gcaaggcgat ctacatcatc gtgcggacac cttccggtgc
     3301 cgccgactcg acgccgatca ccttcactcc cacgccgacc gctctcgcga tacaggcggg
     3361 gaacaaccag accgccacgg tgggtggcct gctcccggct cctctgcagg tccgggtcac
     3421 ggcggcggat ggcctgggag tcaaaggcgt tccggtgcag ttcacgattc tcaccggagg
     3481 cggatcgatc gcgtcggcac cggtggtgac cgacacgggc gggtacgcgt ccgtcacgcc
     3541 gacgctcggg accctcgccg gcgccgcgac ctaccaggcg agcgc
//
LOCUS       DASUBP010000305         3911 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1049065, whole genome shotgun sequence.
ACCESSION   DASUBP010000305 DASUBP010000000
VERSION     DASUBP010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3911)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3911)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3911
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049065"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            76..3027
                     /locus_tag="VFU75_11615"
     CDS             76..3027
                     /locus_tag="VFU75_11615"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HEU5467531.1"
                     /translation="MTKAPWVLPVLLFSFTTAALGQTRVISGRVTASVSGDPLPGVTV
                     AVVGTQSLALTQSDGRFSIGAPPGDVHLLFRKIGYQHKDVPVAAGQDSVSVTMDIDVF
                     VLDAVVVTGQATSVERRNATTGNTVVMATDIGTQPAQTVDKALQGKVPGAIISQNSGA
                     PGGGVQVQLRGVNTVLGNPDPLYVVDGVIYSNVSVPSGLSTATSSGSNRGSGQLQDDP
                     VNRLADLNPNDIESIQVLPGAAASAIYGSKAANGVIIVRTKRGQAGRTAATITQRLGY
                     SELLRGYGTRSFTVAEAESLYGVSAVTPYIVNGALPTYDHMQELAGGKPMSYQTSLEV
                     TGGDERTRYFLSGSTSQDNGIIANTGAGRQTLRLNIDQTFSDRLKLGFSSAFNRTQTD
                     RGFTNNDNAGASVTYSIAYIPGFEPLQPVNGVYPQPSITYLSSNPLQTAALATNQEVA
                     LGFTGGGQLTYQALTTDRHNLQIIAGGGIDVFNQKNTVIAPPELYFQQAQANPGVSTL
                     SNADARYWNWNLNLIDAFTPTSASYRLTTSAGVQVEDRELSRNRLSAFGLLPGETNID
                     QGSVIGNVFELNTIDRTLAFYAQDELLARDDRLAVTGSIRAERSSANGATDRYFIYPR
                     LSASYRFPDLLGAGSDFKIRAGYGETGNQAAFGQKFTTLDAGVYAKHVTTNVDPTAGD
                     AGLRPERVREIEGGFDASLPNGRGSIELTAYRRHTTDVFLSRPPAPSTGFSTQIINAG
                     ELMNEGIELGVGYTPIQTRDLTWVSRINFNTARNEVVSLPFPGFRPPNAGFGLAFGEF
                     FLQPGKPTTQIIGQIGIDQATGNFIVGYMGQANPKFVMSFPQDIRYKRLSLNMLLTWQ
                     YGGVAQNQTLSLYDCNGLSPDQASAAGQARFNDCGIGIATPFVQTTSFLRMQNLSLTY
                     EAPESWYRYFGGASSLRFTLSAQNLFLITDYTGYDPQVSNYGEQAVVRNIDLGPYPPS
                     RSFTFTISAGF"
     gene            3030..>3911
                     /locus_tag="VFU75_11620"
     CDS             3030..>3911
                     /locus_tag="VFU75_11620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467532.1"
                     /translation="MKNSTRSLLILAIALLGGACQNFFDVPQTNQPTLDDLLNHPTRD
                     KLAAAANGIFAGASGDIQSFIWRVGSMGREGINLSGNNTPDYSEPYFGPLSGSGFGAA
                     IWGGRYANIRSINIYLQALAKTTQLSAGEVEASRGMAQTFKALALLYVIETRAQLGAP
                     VDVDRPVTAPPAPFVSEDSVYGYIIGLLDSARTNLAAANTASAAFPFSIPPGLSPFGD
                     PGSFILFNRALAAKSEVFRATAQNGCHGTPATCWNAAETALAASFYAPGDSTQFNAGA
                     YFDFSTDGGDATNDLSDP"
BASE COUNT          690 a         1348 c         1222 g          651 t
ORIGIN      
        1 cacttcccgc gccgcgtcca tgctaaagcc cactccagcc ggtcagttcg ttcacgtctg
       61 caccaggggg tttgcgtgac gaaggcgccg tgggttctgc ccgtcctgct gttctcattc
      121 actaccgccg cgcttggaca gacgcgcgtc atctcgggtc gggtgacggc gtccgtcagc
      181 ggcgacccgc tgcccggcgt gacggtggcg gtcgtcggca cccaatcact cgcgctcacg
      241 cagagcgacg ggcgcttcag catcggtgct ccgccggggg atgtgcatct gctcttccgc
      301 aagatcgggt accagcacaa ggacgtgccg gtcgcggcgg ggcaggactc cgtgtcggtg
      361 acgatggaca tcgacgtctt cgtgctcgat gccgtggtgg tgaccggaca ggcgaccagc
      421 gtcgagcgcc ggaacgcgac gaccggcaac acggtcgtca tggccaccga tatcggcacg
      481 cagcccgcgc aaacggtcga caaggctctg caggggaagg tgccgggagc gatcatcagc
      541 cagaactccg gagcaccggg tggaggcgtg caggtacagc tccggggcgt gaataccgtg
      601 ctggggaatc ccgatccctt gtacgtcgta gacggcgtga tctacagcaa cgtctccgtg
      661 ccttccggcc tctcgacggc gacctccagc ggcagcaacc gggggagcgg tcagttgcag
      721 gacgaccccg tcaaccggct cgcggatctg aaccccaacg acatcgaatc catccaggtg
      781 ctccctggcg cggcggcctc ggcgatctac ggctccaagg cggccaacgg ggtcatcatc
      841 gtccgcacca agcgggggca ggcgggccga acggccgcga ccatcacaca gcgcctcggc
      901 tacagtgagt tgctgcgcgg gtacgggacg cgctcattca ccgttgccga agcggagtcg
      961 ctgtacggcg tcagcgccgt gaccccctac atcgtgaacg gcgcgctgcc gacgtacgat
     1021 cacatgcaag agctggcggg cggcaagccc atgtcctatc agacgtctct ggaggtcacg
     1081 ggcggagacg agcgcacccg gtatttcctc tccggctcga cctcgcagga caacgggatc
     1141 atcgccaaca ccggggcggg ccggcaaacg ctgcggctga acatcgacca gacgttttcg
     1201 gaccgcctga agctggggtt ctcgagtgcg ttcaaccgga cgcagacgga ccgcgggttc
     1261 accaacaacg acaacgccgg ggcgagcgtc acctactcca tcgcctacat tcccggcttc
     1321 gagcccctgc agccggtgaa cggcgtgtac ccgcagccgt cgatcacgta cctgtcgtcg
     1381 aatcccctgc aaacggcggc gctggcgacg aaccaggagg tcgcgctggg gttcacggga
     1441 ggtggccagc tgacctatca ggcgctgacg accgaccggc acaacctcca gatcatcgcg
     1501 gggggcggca tcgacgtgtt caaccagaag aacacggtga tcgcgccgcc cgagctctac
     1561 ttccagcagg cgcaggcgaa tcccggcgtc tcgacccttt ccaacgcgga cgcccgctac
     1621 tggaactgga acctcaacct gatcgacgcg tttacgccga cgtccgcctc gtaccgcctg
     1681 acgacgtccg ccggggttca ggtggaggac cgggagctgt cccgcaatcg cctgagcgcc
     1741 ttcgggctcc tgcccggtga gacgaacatc gatcagggga gcgtcatcgg gaacgtattc
     1801 gagctcaaca ctatcgaccg gaccctggcg ttctacgcgc aggatgagct gctggcccgc
     1861 gacgaccgcc tggcggtgac ggggtcgatt cgcgccgagc gctcgagcgc caatggcgcc
     1921 accgaccggt acttcattta cccccggcta tcggcctcgt accggttccc cgatctcctg
     1981 ggtgcgggaa gcgacttcaa gatccgggcg ggatacgggg agaccggaaa tcaggccgcc
     2041 ttcggccaga agttcaccac gctggacgcg ggggtgtacg ccaagcacgt caccaccaac
     2101 gtcgatccga ccgccggtga tgccgggctc cggcccgaac gcgtccggga aatcgaggga
     2161 ggcttcgacg cctcgcttcc gaacgggcgg ggaagcatcg agttgacggc gtaccggcgg
     2221 cacaccacgg acgtgttcct gagccgcccg ccggccccct cgaccgggtt ctccacccag
     2281 atcatcaatg ccggagagct catgaacgag ggcatcgagc tgggagtcgg ctacacaccg
     2341 atccagaccc gcgatctcac gtgggtgagc cggatcaact tcaacacggc gcggaacgag
     2401 gtcgtctcat tgccgttccc gggcttccgg cccccgaatg ccggatttgg cctggccttc
     2461 ggtgagttct tcctccagcc ggggaagccc acgacgcaga tcatcggcca gatcgggatc
     2521 gaccaggcca ccggcaactt catcgtcggt tacatggggc aggccaatcc caagttcgtg
     2581 atgtcgttcc cgcaggacat ccgctacaag cggctgtcgc tcaacatgct gctgacctgg
     2641 cagtatggcg gcgtcgcgca gaatcagaca ctctcgctgt acgactgcaa cgggctctcg
     2701 ccggatcagg cgagcgcggc ggggcaggcg cggttcaacg actgcggcat cggcatcgcg
     2761 acgccgttcg tgcagacgac ctccttcctc cggatgcaga atttgtcgct gacctacgag
     2821 gctcccgagt cctggtaccg gtatttcggc ggggcgagct cgctccggtt cacgctctcc
     2881 gcgcagaacc tgttcctgat caccgactat accggctacg atccgcaggt gtcgaactat
     2941 ggcgaacagg cggtggtccg gaacatcgat ctcggaccct atccgccctc ccggtccttc
     3001 acgttcacga tctcggcggg gttctgacca tgaaaaattc cacccgctca ctcctgatcc
     3061 tggcgatcgc cttgcttggc ggtgcctgcc agaacttttt cgacgtacca cagacgaacc
     3121 aacccacgct cgacgacctc ctcaaccatc cgacgcgcga caagctcgcc gccgcggcga
     3181 acggcatctt tgcgggcgcc tcaggagaca tccagagctt catctggcgg gtcggctcga
     3241 tgggacgtga gggcatcaat ctgtctggca acaacacccc ggattactcg gagccgtatt
     3301 tcggtcccct gtcggggtcc gggttcggcg cggcgatctg gggaggtcgc tacgcgaaca
     3361 tccggagcat caacatctat ctccaggccc tcgcgaagac gacgcagctc tccgcgggag
     3421 aagtcgaggc ttcgcgggga atggcccaga ctttcaaggc cctcgccctc ctctacgtca
     3481 tcgagacccg cgcccagctc ggcgccccgg tggacgtgga tcgcccggtg accgctcctc
     3541 cggcgccctt cgtgagcgag gactcggttt acgggtacat catcggcctc ctggactcgg
     3601 cccggaccaa tctcgcggcc gccaacacgg cctcggcagc attcccgttc tcgatcccac
     3661 ctggcctgtc cccgttcgga gacccgggga gctttatcct cttcaaccgg gcccttgcag
     3721 ccaagtcgga ggtgttccgg gccacggcgc aaaacggttg ccacggcacc cccgcaacct
     3781 gctggaacgc ggccgagacc gccctcgcgg cttcgttcta tgctccggga gactccacgc
     3841 agttcaacgc gggcgcgtac ttcgactttt ccacggacgg gggcgacgcc accaacgatc
     3901 tctccgaccc g
//
LOCUS       DASUBP010000306         3848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_735504, whole genome shotgun sequence.
ACCESSION   DASUBP010000306 DASUBP010000000
VERSION     DASUBP010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_735504"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..956)
                     /locus_tag="VFU75_11625"
     CDS             complement(<1..956)
                     /locus_tag="VFU75_11625"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5467533.1"
                     /translation="MTAFRRLRGALARILGFGTRADDEARMHEEMSEHLEQRTRQLIE
                     QGLSPTEARRRAGVAFGARASHQEAARDQLRAPWADHLLRDLRFAVRTLFRTPFVTTV
                     AILSLSLGIGASSAIFSLFDEVLLRPLPVREPGRLVNLSAPGPKPGYASCGEPGDCQV
                     TFSYLMFRDLEQRQTVFSDLAGHVPFSASVTYQDQPLTGDAVLVSGSYFPTLGLQPAL
                     GRLLSESDDQSIGTNFVAVLSYDYWQSRLGGDRAIVGKPITVNGRALTIVGVAEPGFE
                     GTTLGLQPLVYVPLTMQSVLVPNGEAFEDRRDYWVYAFARLKP"
     gene            complement(953..1297)
                     /locus_tag="VFU75_11630"
     CDS             complement(953..1297)
                     /locus_tag="VFU75_11630"
                     /inference="COORDINATES: protein motif:HMM:TIGR03433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEU5467534.1"
                     /translation="MATRDSRDDVLQGTLDMLVLKALSLEPMHGWGISDRLQQWSQDV
                     LQLNEGSLYPALYRLERQGLVRSAWKPSANNRRARYYELTAAGRRQLELELRAWARAS
                     GAVNLVLAVQTP"
     gene            1448..1801
                     /locus_tag="VFU75_11635"
     CDS             1448..1801
                     /locus_tag="VFU75_11635"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HEU5467535.1"
                     /translation="MPHYLIAGYLPKDFDPSTMTEAMVDGIHALNREMVAAGVRRFAG
                     GLGPAHTLKTQPNGEVVVTDGPYLETKELVGGITILEVANLEEALKWARKGAKVSRMT
                     SEVREIFFMPAPEKG"
     gene            1821..3086
                     /locus_tag="VFU75_11640"
     CDS             1821..3086
                     /locus_tag="VFU75_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015895064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEU5467536.1"
                     /translation="MGDVNRTIEAVWKIESTRLIAAIARVTRDVGLAEELAQDALVVA
                     LERWPDEGIPANPAAWLMTVAKRLAIDSLRRGQMLVQKHEDIARELEVQQRRIGEEMD
                     QALDQVIDDDILRLIFTACHPILAVEGRIALTLRLVAGLTTTEIARAFLVPEKTMGQR
                     IFRAKKTLSEAHVPFETPRGDDLRRRVTSVLSVVYLLFNEGHTATSGEEWMRAALCDE
                     ALRLGRMLVQLLPGESEVHALLALMELNASRTAARRGTNGEPVLLADQDRSRWDAAQI
                     QRGLAALEHAQRLGGGAEYYVLQAAIAACHVRARTAEETDWERIVLLYDALMQITPSA
                     IVALNRAVAVGMGQGPAAGLAAVDAIATDAGDAALARYHLYHSVRGDLLMKLGRLPEA
                     REQIQRAIALTQNLREQELLKERLKQMDQ"
     gene            complement(3080..3685)
                     /locus_tag="VFU75_11645"
     CDS             complement(3080..3685)
                     /locus_tag="VFU75_11645"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HEU5467537.1"
                     /translation="MVRLLPFEFTGGNPCLDFSNTVDDRGSDHPQERLTDYARLVQWG
                     SEAGVITGQVAERLRRLATAAPGPAAGALRKAIHLRETIYEIFSAVAQRRTIPAQALA
                     ALNESARQASEHAQLVQTNRGVASEWLDPERHLDAMLWPVSRAATELLLGGELDYVRQ
                     CASETCAWLFHDQSKNHRRRWCDMKVCGNRDKARRYYQRRH"
BASE COUNT          672 a         1200 c         1355 g          621 t
ORIGIN      
        1 ggtttgagcc gggcgaatgc gtacacccaa taatcccgcc ggtcttcgaa ggcctctccg
       61 ttgggtacca ggacgctttg catcgtgagg ggcacgtaca cgagcggctg cagaccgagc
      121 gtggtgcctt caaaccccgg ctcggcgaca cccacgatgg tcagcgcccg tccgttcacc
      181 gtgatcggct tcccgacgat cgcgcgatct ccgccgagtc gcgactgcca gtagtcgtag
      241 gacagcacgg cgacgaaatt cgtcccgatg ctctggtcgt cgctctcgga caagagacgt
      301 ccgagggcgg gttggaggcc cagggtcggg aagtacgacc cggagaccaa taccgcgtct
      361 ccggtgagcg gttgatcctg gtatgtcacg ctcgcgctga acgggacgtg gccggccaga
      421 tccgaaaaca ccgtctgccg ctgctcgagg tcccggaaca tcaggtagct gaaggtcacc
      481 tggcagtcac ccggctcccc gcaggacgcg tacccgggct tggggccggg agcgctgagg
      541 ttgacgaggc ggccaggctc ccggactggc agggggcgca ggagcacctc gtcgaagagc
      601 gaaaaaatcg ccgagctcgc gccgatcccg aggctcagcg agaggatggc aaccgtcgtg
      661 acaaagggag tccgaaagag ggttcggacc gcgaagcgca ggtcccggag gagatggtcg
      721 gcccagggtg cccgaagctg atcccgagcc gcttcctggt gcgaggcgcg agcgccgaag
      781 gcgacgccgg cgcgtcggcg ggcctccgtc ggtgacaggc cttgctcgat gagctgacgc
      841 gtccgttgct ccagatgttc ggacatctct tcgtgcatgc gcgcttcgtc gtccgcgcga
      901 gtgccgaatc cgaggatgcg ggcgagggcg ccgcgcaggc gccggaaggc ggtcacgggg
      961 tctggaccgc gagcacgagg ttgacggcgc cggacgcgcg agcccatgcg cgcagctcca
     1021 gctccagctg gcggcggccg gcggcggtca gctcgtagta ccgggcgcgc cggttgttgg
     1081 cgctgggctt ccacgccgag cgtaccaggc cttggcgttc gaggcggtag agggcggggt
     1141 agagggaccc ctcattcagc tggaggacgt cctgcgacca ttgctggagc cggtcgctga
     1201 tgccccagcc gtgcatcggt tcgagggaca aggccttcag caccagcatg tcgagggtgc
     1261 cctggaggac gtcgtccctg gagtctcgcg tcgccacgtc atgtcccctt agatgttcga
     1321 agggagcata gggttgctcc cttagagcgt caaggggtgg ggactggtag aattccggcc
     1381 tcctcattcg tcgtcatatg tagaagccgg gcaatgcgcc cggcaggcca ccacctcagg
     1441 agacgacatg ccgcactatc tgattgccgg gtacctcccc aaggacttcg acccctccac
     1501 gatgaccgaa gcgatggtgg acgggattca cgcgctcaat cgcgaaatgg tcgcggccgg
     1561 ggtgcggagg ttcgccgggg ggttgggccc ggcccacacg ctcaagacgc aacccaatgg
     1621 tgaggtggtg gttaccgacg ggccctacct cgaaaccaag gagctcgtgg gcggcatcac
     1681 gatcctggaa gtcgccaacc tggaggaggc gctcaaatgg gcccgcaagg gcgccaaagt
     1741 gagccgcatg acgagcgagg tccgagagat tttcttcatg cccgcccccg aaaagggctg
     1801 accatcagga cgggaggtcc atgggggatg taaacaggac gatcgaggcc gtgtggaaga
     1861 tcgagtccac gcggctgatc gccgccattg cccgcgtcac ccgcgatgtc ggccttgccg
     1921 aggagctcgc ccaggacgcg ctggtggtcg cgctcgagcg gtggcccgac gaaggcatcc
     1981 ccgccaatcc cgcggcgtgg ctcatgacgg tggccaaacg gctcgccatc gacagcctcc
     2041 gccgcgggca gatgctggtt cagaagcacg aggacattgc ccgggagctc gaggtccagc
     2101 agcggcggat cggtgaggag atggatcagg cattggacca ggtcatcgac gatgacatcc
     2161 tgcggctgat cttcacggcc tgccatccga tcctcgcggt cgaaggacgg atcgcgctca
     2221 ccctgcgtct ggtcgccggc ctcaccacga cggagatcgc ccgggccttt ctggtaccgg
     2281 aaaagacgat gggacagcgg atcttccgcg cgaagaagac gctgtccgaa gcgcacgtcc
     2341 cctttgagac gccgcgggga gacgacttgc gccgcagggt gacgtcggtg ctttccgtgg
     2401 tctacctgct cttcaatgag gggcacacgg cgacgtcagg ggaggagtgg atgcgggcgg
     2461 cgctgtgcga cgaggcgttg cggctcggcc ggatgctcgt ccaactcttg ccgggcgagt
     2521 ccgaggtgca cgcgctgctg gcactgatgg agctcaatgc ctcacgcacc gcggcgcgcc
     2581 gaggaaccaa cggggagccg gtgttgctgg cggatcagga ccgctcgcgg tgggatgccg
     2641 cgcaaatcca gcgcggcctg gccgcactcg aacatgcgca gaggctgggt gggggcgcgg
     2701 aatactacgt gttgcaggcg gcgattgccg cgtgtcacgt gcgcgcccgc accgccgaag
     2761 agaccgattg ggaacggatc gtgctcttgt acgacgccct gatgcagatc acgccctctg
     2821 ccatcgtcgc cctcaaccgt gcggtggcgg tgggtatggg gcaggggccc gccgcgggac
     2881 tggccgccgt ggatgccatc gccacggatg cgggggacgc cgcgttggcg cggtatcacc
     2941 tgtatcacag cgtgcgggga gacctgctga tgaaactggg acggctgccg gaggcgcggg
     3001 agcaaatcca gcgcgccatc gcccttaccc agaatctccg ggaacaggag ctcctgaagg
     3061 agaggctgaa gcagatggat cagtgacgcc gctgatagta cctgcgggct ttgtcgcggt
     3121 tgccgcagac cttcatgtcg caccagcggc gccggtggtt cttcgactgg tcgtggaaga
     3181 gccaggcaca ggtctcggac gcacactgcc gcacgtagtc cagctcgccg ccgagtagca
     3241 gctcggtggc cgcccggctc accggccaca gcatcgcgtc gaggtgacgc tcggggtcga
     3301 gccattccga cgcgactcca cggttggtct ggaccaattg cgcgtgctcc gaggcctggc
     3361 gggctgattc gttcagggcg gcgagggcct gggcggggat ggtccgtcgc tgcgccacgg
     3421 ccgagaagat ctcgtaaatc gtttcgcgga ggtgtatcgc ctttcgcaaa gcgcccgccg
     3481 ccgggccggg cgccgcggtc gccagacggc gcagccgctc ggccacttgg cccgtgatca
     3541 ccccggcttc cgacccccat tgcacgaggc gcgcgtagtc ggtgaggcgc tcctggggat
     3601 ggtcactccc gcggtcatcc accgtgttgg agaaatcgag gcagggattc cccccggtga
     3661 attcgaaggg caggagccta accatcaaaa tggccttgac aggtcataca ccgcttggat
     3721 agcatatcac cagtataaat acttttggtg gttatgtgaa cccgaagagt ctcgagccca
     3781 tcaccatcgc catgatcatc gggggcgggg tgctctatca cgtcagccag aaggccacgc
     3841 cggcgggg
//
LOCUS       DASUBP010000307         2799 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_653415, whole genome shotgun sequence.
ACCESSION   DASUBP010000307 DASUBP010000000
VERSION     DASUBP010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_653415"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            73..1254
                     /locus_tag="VFU75_11650"
     CDS             73..1254
                     /locus_tag="VFU75_11650"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5467538.1"
                     /translation="MLRIRAGRVHPVTAPPIEDGAVLVDDGGKITAVGPQSRIPHPPG
                     SESFEFRDAVLVPGFVNTHTHLELTHLTGKNGPEQSFPRWIRRVRALKEETPAEQFDA
                     AAEQGLRDCWAQGVTCIADTGDSGAAIRALFRLGGRGIAYHEVFGPDPAQADPSLEGL
                     KKALHELRRFTSFEASLGVSPHAPYTVSAPLYRAVAEYAMRDGLPIAVHLAESKEESA
                     FVLDGEGPFAAAWGDRGIPVEARRMSPVQFLHSLGVLRLGTLCIHTVQLDAQDIATLA
                     ASKVGVAHCPLSNRAHGHGTAPLAELRKAGVPVGLGTDSVVSVGALDLWGEAQAAGLE
                     GEEALRMLTFEGARALCMENVVGSLEVGKQGDVAVLSDLATERPGVLATVLSGRIVYR
                     T"
     gene            1275..1730
                     /gene="argR"
                     /locus_tag="VFU75_11655"
     CDS             1275..1730
                     /gene="argR"
                     /locus_tag="VFU75_11655"
                     /inference="COORDINATES: protein motif:HMM:TIGR01529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006525 - arginine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEU5467539.1"
                     /translation="MKTQRHAAILKVIRRDTVGSQEQLRELLSAEGFDVTQATLSRDI
                     RELGLAKVAAPDGASYYSPPDEVGAAPRPHLEQLLPTMLVSVDGVGPLLVLKTATGAA
                     QPLAVAVDGSGWSEIIGTIAGDDAILVITKSERARRAIQTRIEELAGLS"
     gene            1808..>2799
                     /locus_tag="VFU75_11660"
     CDS             1808..>2799
                     /locus_tag="VFU75_11660"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEU5467540.1"
                     /translation="MGPLVVLAYSGGLDTSAILPWLRDTYGARVLCFAADVGQGGGEL
                     HGLVEKAKRSGAVDCVIEDLREPFVKDFVFPTLKAGAIYARTYLLGTSMARPITAAHQ
                     VAAARRAGAQALAHGCTGKGNDQVRFELTYAALAPDLQVIAPWREWTFKGREELIAYV
                     KANNIPIQATLEKPYSTDRNLWHCSHEGGILEDPSLEAPEHIFVMTRDPITAPDQPQI
                     VQIGFDAGIPVTVDGEALSPVKLLEVLNEIAGEHGIGRADVVEDRLVGMKSRGVYETP
                     GGTLLYAAHRELEQLVLDRRTLKMKDELALHYADLVYDGRWWTPEREALDAAVNT"
BASE COUNT          460 a          960 c          924 g          455 t
ORIGIN      
        1 ctatcccggc accgagccta ccaagctgtg cccggtccac tcggggctcg gcaccggcat
       61 caacccctga ccatgctccg cattcgcgcg gggcgcgtac accccgtcac ggcgccgccc
      121 atcgaggacg gcgccgttct cgtagacgac ggcgggaaaa tcaccgccgt cggcccccaa
      181 tcccgaatcc cccatccccc ggggtcggag tccttcgaat tcagggatgc ggtgctcgtc
      241 ccggggttcg tcaacaccca cacgcacctc gagctgacgc acctcacggg gaaaaacggt
      301 cccgagcagt cattcccgag gtggatccgg cgggtgcggg cgctgaagga agagaccccg
      361 gcggagcagt tcgacgccgc ggcggagcag gggctccgtg actgctgggc gcagggtgtc
      421 acctgtatcg ccgataccgg ggactcaggt gcggccattc gcgccctgtt ccggctggga
      481 gggcggggaa tcgcctacca cgaggtgttc ggccccgatc cggcgcaggc tgaccccagt
      541 ctcgagggcc tgaagaaggc actgcacgag ctgcgtcgct tcacgtcgtt cgaggcctca
      601 ctcggcgtct caccgcacgc cccctatacc gtcagcgcgc cgctctaccg cgccgtggcc
      661 gaatacgcga tgcgagacgg attgccgatc gccgtccacc tggccgagtc gaaggaggag
      721 tcggcattcg tgctggacgg tgagggtccc ttcgccgccg cctggggaga ccgggggatt
      781 cccgtagaag cccgccgcat gtcccccgtg caattcctgc attcactcgg cgtcctgcgg
      841 ctggggacgc tctgcatcca caccgtacag ctcgatgccc aggacatcgc caccctggcg
      901 gcgagcaagg tcggagtcgc ccactgcccg ctctccaacc gggcccacgg ccatggcacc
      961 gccccgctcg ccgagctgcg caaggcgggc gtgcccgtcg gcttggggac ggattcggtg
     1021 gtgagcgtcg gggcgttgga cctgtgggga gaggcacagg cggccggact ggaaggcgag
     1081 gaagcgctgc gcatgctgac cttcgaaggc gctcgtgctc tttgtatgga gaacgtggtc
     1141 ggctctctgg aggtcgggaa acagggagac gttgcagtac ttagcgactt agcgactgag
     1201 cgacctggcg tcttggcgac tgttctttca ggccgcatcg tctatcggac ttgagaggga
     1261 tacatttccc gcgaatgaaa acccagcgcc acgccgccat tctgaaagtg atccgccgcg
     1321 acactgtggg cagtcaggag cagctccggg agctcctgtc cgccgagggg ttcgacgtga
     1381 cccaggcgac gctctcgcgg gatatccggg agctgggact cgccaaggtg gcggcccccg
     1441 acggcgcgtc ctattactcg ccccccgacg aagtcggtgc ggctccccgg ccgcacctgg
     1501 agcagctgct ccccaccatg ctcgtctccg tcgacggtgt cgggccactg ctcgtattga
     1561 aaaccgcgac cggtgcggcg cagccgctcg ccgtcgcggt ggacggctcc ggctggagcg
     1621 agatcatcgg taccatcgcc ggtgacgacg ccattctcgt gatcacgaag agcgaacgcg
     1681 cccgccgcgc catccagacc cgcatcgagg agctcgcggg tctttcctga tcgcccttgc
     1741 gcccgcctgc cccctctgta tgtttatgca tcgggatgca taaacctcca actcgggtgg
     1801 cgcactgatg ggtcctctcg tcgtcctcgc gtattccggc ggtctcgata cctccgccat
     1861 tcttccctgg cttcgggaca cctatggtgc ccgggtcctg tgctttgccg cggacgtggg
     1921 gcagggcggt ggtgagctgc acggactggt cgagaaggcc aagcgctcgg gtgccgtgga
     1981 ttgcgtgatc gaggacttgc gagagccgtt cgtcaaagac ttcgtcttcc ccacgctcaa
     2041 ggccggcgcg atctacgcgc ggacatacct cctgggcacc tcgatggccc ggcccatcac
     2101 cgcggcgcat caagtcgccg cggcgcgccg cgcgggcgcg caggcgctgg cgcacgggtg
     2161 caccggcaag gggaacgatc aggttcgctt cgagctgacg tacgccgctc tggcgcccga
     2221 cctccaggtc atcgcaccgt ggcgggagtg gaccttcaag ggccgggaag agctgatcgc
     2281 gtacgtgaag gcgaacaaca tcccgatcca ggccacgctc gaaaagccgt attccaccga
     2341 ccgcaatctc tggcactgct cgcatgaagg cgggattctc gaagatccct ccctcgaggc
     2401 gccggagcac atcttcgtga tgacgaggga tccgatcacc gcgcccgacc agccacagat
     2461 cgtgcagatc gggttcgacg cgggcattcc ggtgacggtg gacggcgagg ccctgagccc
     2521 cgtcaagctc ctcgaggtcc tgaacgagat cgccggcgag cacggcatcg gccgcgccga
     2581 cgtcgtggaa gaccggctgg tgggcatgaa gtcgcggggc gtctatgaga cccccggcgg
     2641 cacactgctc tacgccgcac accgcgagct cgagcaactg gtgctcgacc gccggaccct
     2701 gaagatgaaa gacgagctcg ctctccacta tgccgacctg gtctacgacg gccgctggtg
     2761 gacccccgag cgcgaagcgc tcgacgcggc cgtgaacac
//
LOCUS       DASUBP010000308         6458 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_680988, whole genome shotgun sequence.
ACCESSION   DASUBP010000308 DASUBP010000000
VERSION     DASUBP010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 6458)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6458)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6458
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680988"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1450
                     /locus_tag="VFU75_11665"
     CDS             <1..1450
                     /locus_tag="VFU75_11665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5467541.1"
                     /translation="TCAAIGVDAGDLDLPVPLDKRSYRAAVTLLTDRGLVENGRLTNY
                     GRQVEAMPCERPWGELLVHADTPLLPVVAVAANIDSLHRMTRDDRNLQGLIVPGSDHL
                     TAYNVFAEAVNKHGDLGEVYGLPRHLFRETIDRWAEGRGVLVKAIEDIALGTASVYRQ
                     LEMSLPARFPVADDAIKFQFADLLAKVAPFDVVIDEETADGQEARVSRGSVCGSWGAI
                     AGTLRYFADRFGNPRAAIEGTQIPERSLRRYARRAPPQITFERHRRHEGLMAVRTVEY
                     QGFMLEREVEALTGTFPADLAEAARQALVKAVRNGGTPHPDQPRLSRAWHRAQALWTR
                     SGGTLSQLAPEALEERLAHQMASVTSWDSFLRARLSLEVDDLVSEAERARLDGLPQSV
                     ALLGDRVAIEYGIEDGRGVARLHLKEGQARRIRESDLPVLDRPLRFTVTRGKREAARA
                     DSIADLKKRLDELPRGKPKSGKPFRPPRRRRR"
     gene            1562..2017
                     /locus_tag="VFU75_11670"
     CDS             1562..2017
                     /locus_tag="VFU75_11670"
                     /inference="COORDINATES: protein motif:HMM:NF014950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEU5467542.1"
                     /translation="MTQNLPSLTPLGRALLDSIREGVVVFDTDGQVLYANQEGQRSIG
                     NGSPNGNLPGKEELLPRLSALGARILPLRVPDSQAGAAAVIPPKADRGRTLAERERQA
                     IIDTLEATQGKLAETARRLGISRTTLWRRLREYGLKQKNEAFWRARAAS"
     gene            2063..2530
                     /locus_tag="VFU75_11675"
     CDS             2063..2530
                     /locus_tag="VFU75_11675"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HEU5467543.1"
                     /translation="MANSPSPFLIQEYVRWSDVDYAGIIFYGAYVRFFEIAETELFRA
                     SGLAYKDVFDRHGIFLPRRAIHAEFFVPARLDDRLTVAAYIGKVGTRSMTINFDVVWG
                     EPRFLGATGWQVLVCVDKQTLKPRAAPEALLQALAPHTLSPEAARAALGVRAP"
     gene            2596..3015
                     /locus_tag="VFU75_11680"
     CDS             2596..3015
                     /locus_tag="VFU75_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467544.1"
                     /translation="MGVRVFRTAKDSTVYTALDVAHLCKGGGVLFDAVSGAYGVLAWV
                     RSPTPLSAGSYPLLGRADSITPRGAVVAIRFILHDIAHGFPVDSGTLTLTAVGSEYRG
                     RIQGSGTDAGLASRSRVDIVIDSVRPRPDSLTCGAKG"
     gene            3012..4076
                     /locus_tag="VFU75_11685"
     CDS             3012..4076
                     /locus_tag="VFU75_11685"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEU5467545.1"
                     /translation="MTVKIGMIGTGFARRVQLPGLKLVPGVEVTGIASGKRANADQVA
                     KDFGIANVFDDGVELARSGVADLIIVASTPDTHLRFGLAALEAGRHLLCEKPTTLAAP
                     EALRLWEASAARPRQVAWMDHELRYEPNRKKVRDLIKSGTIGEIRHIEMALRPYLRGD
                     GRPQASNAPWNWWYDAGRGGGLLGAVGSHLIDLCRFWTASEPVSVAGVVETFTKRRND
                     DAGVSHPVTADEFASWTMRMGSGAVASLTLSSVAHHGGGHWCQITGTEGSLVLTGEHT
                     LHIGKRGGDLEDITQPDDLWEKTTPNNMWARSFVRLMRDLVPVLGGAKPSGTPATMED
                     GWRIQRIMDAVRAGVTARLD"
     gene            4081..5289
                     /locus_tag="VFU75_11690"
     CDS             4081..5289
                     /locus_tag="VFU75_11690"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HEU5467546.1"
                     /translation="MEQTYFRKGFGLKGAIEGTLTADYHSRVVDHVRAQGFSLAVGDL
                     TFRLAREFGFCYGVDRAVEYAYETRTKFPDARVFLVGEIIHNPHVNAKLRSMGVEFLH
                     RPEGGEFDFSHIVATDVVLMPAFGVTVKDFQRLRDLGCVLVDTTCGSVLNVWKRVESY
                     AKDGFTALIHGKAYHEETKATSSQVTKYPGGRYVVVRDMGEARLLCDYIEGRGDHKAL
                     VAKFASAMSPGFDPDRDLVRVGVANQTTMLSGESLAIAAEVAKSMVRRYGEGSLAEHF
                     RTFDTICSATQERQDAVLKLIEDPIDLMVVIGGYNSSNTTHLAAICHERVPTYHIEDA
                     AGIDPDARTIRFRPVGAKTEETKGDWLPAGPLVVGVTAGASTPNNKVGETIERIAAMR
                     GLALEVAPAS"
     gene            5286..>6458
                     /locus_tag="VFU75_11695"
     CDS             5286..>6458
                     /locus_tag="VFU75_11695"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEU5467547.1"
                     /translation="MKQFHPAVERWFTGRFHEPTEPQRRAWPAIQAGRDVLIAAPTGS
                     GKTFAAFLSSINSLLQQGVDGSLDDQIQVLYISPLKALSNDVQKNLSEPLLEISRTAL
                     EMGLPEVTLRTLVRTGDTPQAERQAMLKDPPHILVTTPESLYLLLTSEKARGMLRTVR
                     TVIVDEIHALARDKRGSHLTLSLERLAALTGRPLQRIGLSATQKPIEEIAQFLVGTGR
                     PLPDIIDAGHAREIDLAVELTSSPLEAVMSGEVWGEIHERLTQLVQEHRTTLIFVNTR
                     RMAERLAMHLSERLGENAVTSHHGSLSKETRLDAERRLKAGELKALVATASLELGIDI
                     GTVDLVCQVASTRSIATLLQRVGRSGHHLKAIPKGRLFPLSRDDLAECAALLRAVKS"
BASE COUNT         1073 a         2220 c         2166 g          999 t
ORIGIN      
        1 gacctgcgcc gccatcggcg tggacgccgg ggatctcgat ctgcccgtgc ccctcgacaa
       61 gcggtcgtat cgggcggcgg tcacgctgct caccgaccgg ggcctggtgg aaaacggacg
      121 cctcaccaac tacgggcgcc aagtggaggc gatgccgtgc gagcgtccct ggggcgagct
      181 cctggtgcac gccgacacgc cgctcctccc cgtggtcgcc gtcgcggcga acatcgactc
      241 actccaccgc atgacgcgcg acgaccgcaa cctgcaggga ttgatcgtcc cgggctccga
      301 ccacctcacc gcgtacaacg tcttcgccga agcggtcaac aagcatggcg acctcggcga
      361 ggtgtacggc ttgccgcgcc acctgttccg ggaaaccatc gaccgctggg cggaaggccg
      421 gggcgtgctg gtcaaggcga tcgaggacat cgcgctcggc accgcatcgg tgtatcggca
      481 gctcgagatg tccctccccg cccgctttcc cgtggctgac gacgcgatca agttccaatt
      541 cgccgatctg ctcgcgaagg tcgcaccctt cgatgtcgtg atcgacgagg agaccgcgga
      601 cgggcaggag gcgcgggtga gtcgcggatc ggtgtgcggg agttggggcg ccatcgcggg
      661 gacgctgcgc tatttcgcgg accgcttcgg caatccgcgc gcggccatcg aaggcaccca
      721 gatcccggag cgctccttgc ggcgctatgc ccgccgcgcg ccgccgcaga tcacgttcga
      781 gcgccaccgc cggcacgaag ggctcatggc cgtgcggacc gtcgaatacc agggcttcat
      841 gctcgagcga gaggtcgagg cgctgaccgg aacgttcccg gccgatctgg cggaggccgc
      901 gcgccaggcg ctggtgaagg ccgtccgcaa tggggggacc ccgcaccccg atcagccgcg
      961 tctttcccgc gcctggcacc gcgcccaggc gctctggacc cgttcgggtg ggacgttgag
     1021 ccagctcgcc cccgaggcgc tcgaggagcg gctcgcgcac cagatggcgt cggtgacgag
     1081 ctgggactcg ttcctgcgcg cgcgcctgtc actcgaggtg gacgacctcg tgagcgaggc
     1141 ggagcgggcc aggctggatg gactgcccca gtcggtcgcg ctcctgggag accgggtggc
     1201 catcgaatac gggatcgagg acggaagggg cgtagcgcgg ctgcatctca aggagggtca
     1261 ggcccggcga attcgcgaaa gcgacctgcc ggttctcgac cgcccgctcc ggttcaccgt
     1321 cacccggggg aaacgagagg cggcgcgggc cgactccatc gccgatctca agaagcgcct
     1381 cgacgagctt cccaggggga aacccaagtc ggggaagcct ttccggcccc cccgccggcg
     1441 ccgaaggtag acaaatccgg ggcgtttaca tctgtgacct tcgcccgttc gcggggtctc
     1501 ttgagggaac ccaagagaac cctgcattac ggaaaggtca ccaatggccg gcaaccgtcc
     1561 aatgactcag aatcttccat cgctcactcc cctcggtcgc gcgctcctcg attccattcg
     1621 cgagggcgta gtggtcttcg ataccgatgg ccaggtcctc tacgccaacc aggaaggcca
     1681 acgctccatc gggaacggct cccccaacgg caacctgccg gggaaggaag agctgctccc
     1741 aaggctctcc gcgttgggcg ctcgcatcct gccgcttcgc gtccccgatt cgcaggccgg
     1801 ggcggcggcg gtcattcccc ccaaagccga ccggggccgc acgctcgccg aacgtgagcg
     1861 ccaggcgatc atcgacacct tggaagcgac ccaaggcaag ctcgccgaaa ccgcgcggcg
     1921 tctcggcatc agccgcacga ctctgtggcg ccggctgcgg gagtatggac tgaagcagaa
     1981 gaacgaagcg ttctggcggg ctcgcgccgc ttcctgacgc gcggccttac gtccccggga
     2041 ggccgctcct agcttcccgg ggatggcgaa ttccccgagc ccgtttctca tccaggagta
     2101 cgtccgctgg tcggacgtag actacgccgg catcattttc tacggcgcgt acgtccgttt
     2161 tttcgaaatc gccgagaccg agctcttccg tgcgtcggga ctggcctaca aggacgtctt
     2221 cgaccgccac gggatcttcc tgccgcgccg cgccatccac gccgagttct tcgtgcccgc
     2281 ccgcctggac gaccgcctca cggtggccgc gtacatcggc aaggtcggca cgcgctccat
     2341 gaccatcaat ttcgacgtcg tctgggggga gccccgattc ctgggcgcca ccggatggca
     2401 ggtgctcgtc tgcgtggaca agcagacctt gaagccgcgc gccgcgccgg aagcgctgct
     2461 tcaggccctc gcgccccaca ctctgtcgcc cgaggccgca cgcgccgcgc tgggcgttcg
     2521 cgctccctga agaccgcacc tctgctcggc gccctcctgg gggtcgcggc ctgcctcgac
     2581 agcccgcccg ccaccatggg tgtgcgcgtc tttcgaacgg ccaaggactc gacggtatac
     2641 acggcgctcg acgtcgctca cctgtgcaaa gggggcggcg tcctcttcga cgccgtgagc
     2701 ggggcctacg gcgtgctcgc ctgggtccgt tcaccgaccc cgctctcggc ggggtcgtac
     2761 ccgctcctcg gacgggcgga cagcatcacg ccgcgcggcg ccgtcgtggc gatacgcttc
     2821 atcttgcatg acatagccca cggattcccc gtggattcgg ggaccctgac actgaccgcc
     2881 gtcggatcgg agtatcgggg acggatccaa ggctcgggga ccgatgcggg actcgcctcc
     2941 cggagccggg tggacatcgt gatagactcc gtcaggcccc ggcccgactc actcacgtgt
     3001 ggagcgaagg gatgactgta aagatcggca tgatcggcac gggattcgcc cgccgggtcc
     3061 agctgcccgg gctcaagctc gtccccggcg tcgaggtgac gggcatcgcg agcgggaagc
     3121 gcgcgaacgc cgaccaggtg gcgaaggact tcggaatcgc gaacgtcttc gatgacggcg
     3181 tcgagctggc gcgttccggc gtcgccgatc tgatcatcgt cgcgtcgacc cccgacaccc
     3241 acctgcgctt cggcctcgcc gcgctcgaag cgggacgaca tctgctctgc gaaaagccga
     3301 cgacgctcgc cgcacccgag gccctgcgtc tgtgggaggc gtcggcggcg cgacccaggc
     3361 aggtcgcgtg gatggatcac gagctccgct acgagccgaa ccgcaagaag gtccgcgacc
     3421 tgatcaagag cggcaccatc ggcgaaatcc ggcacatcga aatggccctg cgcccctatc
     3481 tcaggggaga tggcaggccg caggcgagca acgccccgtg gaactggtgg tacgacgcgg
     3541 gacggggcgg cgggctcctg ggggcggtgg ggtcgcacct gatcgatctc tgccgcttct
     3601 ggaccgcgag cgaaccggtg tcggtcgccg gggtcgtcga gaccttcacc aagcgccgga
     3661 acgacgacgc gggcgtgtcc caccccgtga ccgccgacga gttcgcgagc tggaccatgc
     3721 ggatgggatc gggtgcggtg gcgtcactta cgctgtcgtc ggtggcccat catggcggcg
     3781 gccattggtg ccagatcacc ggaacggaag ggtcgctggt gctcaccggg gagcacaccc
     3841 tgcacatcgg caagcgcggc ggggacctcg aggacatcac gcagccggac gacctctggg
     3901 aaaagaccac gcccaacaac atgtgggccc ggtcgttcgt ccggctgatg cgcgacctgg
     3961 tgcccgtact gggtggcgcc aagccttccg gcacgccggc cacgatggag gatggctggc
     4021 ggattcagcg gatcatggac gccgtgcgcg ccggagtgac tgcccggtta gattagggcc
     4081 gtggagcaga cgtactttcg gaagggtttc ggactcaaag gggccatcga aggcaccctg
     4141 accgcggact accacagccg cgtcgtagat cacgtacgtg cgcagggttt cagcctcgcc
     4201 gtcggtgacc tgaccttccg cctcgcccgc gaattcggct tctgttacgg cgtcgaccgc
     4261 gcggtcgaat acgcttacga gacccgcacc aagttccccg acgcgcgggt cttcctggtc
     4321 ggcgagatca ttcacaatcc ccacgtcaac gccaagctcc gctccatggg cgtggagttt
     4381 ctccaccgcc ccgagggcgg agaattcgat ttctcccaca tcgttgcgac cgacgtcgtg
     4441 ctcatgcccg cgttcggcgt gacggtgaag gacttccagc gcctccgcga cctgggctgc
     4501 gtgctggtgg atacgacctg cggatcggtg ctcaacgtgt ggaagcgggt cgagtcgtac
     4561 gccaaggacg gcttcacggc gctgattcat ggcaaggcct atcacgaaga gaccaaggcc
     4621 acctcgagcc aggtgaccaa gtatccgggt gggcgctacg tcgtggtccg ggacatgggc
     4681 gaggcccgct tgctctgcga ttacatcgaa gggcggggcg accacaaggc gctggtcgcc
     4741 aagttcgcga gcgcgatgtc ccccggcttc gacccggacc gcgatctggt gcgggtgggg
     4801 gtggcgaatc agaccacgat gctgtccggt gagtcgctcg cgatcgccgc cgaggtcgcg
     4861 aagagcatgg tgaggcgcta cggcgagggc tcactggccg agcacttccg cacgttcgac
     4921 accatctgct cggcgacgca ggaacgccag gacgccgtgc tgaagctgat cgaggacccg
     4981 atcgacctga tggtcgtgat cggcggctac aactcgagca acaccaccca cctcgcggcg
     5041 atctgccacg agcgcgtacc cacgtatcac atcgaagacg ccgcgggcat cgatcccgac
     5101 gcccggacca tcaggttccg tccggtgggc gccaagaccg aagagaccaa aggcgactgg
     5161 ctgccggccg gaccgctggt ggttggtgtc accgccggcg cgtcgactcc gaacaacaag
     5221 gtcggcgaga ccatcgagcg gatcgcggcc atgcgcggcc tggcgctcga ggtggcaccg
     5281 gcatcgtgaa gcagttccat cccgcggtgg agcgctggtt caccgggcgc ttccacgaac
     5341 cgaccgagcc gcaacgccgc gcctggcccg ccattcaagc gggccgcgac gtcttgatcg
     5401 ccgcgcccac cggctccggc aagaccttcg cggcatttct gtcgtccatc aactcgctcc
     5461 tccagcaggg ggtggacggg tcgctcgacg accagatcca ggtcctctac atctcgcccc
     5521 tgaaggcgct ctccaacgac gtccagaaga acctgagcga gcccctgctc gagatcagcc
     5581 gcacggcgct cgagatgggt ttgcccgagg tgacgttgcg cacgctggtg cgcaccggag
     5641 acacgccgca ggcggagcgg caggcgatgc tcaaggaccc gccccacatc ctggtgacga
     5701 cgccggagtc gctgtacctg ctcctgacgt ccgagaaggc ccgcgggatg ttgcggacgg
     5761 tgcggacggt gattgtggac gagatccacg cgctcgcgcg cgacaagcgg gggagccacc
     5821 tgaccctgtc actggaacgg ctcgccgccc tcaccgggcg cccgctccag cgcatcggcc
     5881 tttcagctac gcagaagccg atcgaggaaa tcgcccagtt cctggtcggt acgggccggc
     5941 cccttcccga catcatcgac gccggacacg cccgcgaaat cgatctcgcg gtcgagctca
     6001 cgagctctcc cctggaagcc gtgatgtcgg gggaggtctg gggggagatc catgaacggt
     6061 tgacgcagct ggtccaggag caccggacca cgctgatctt cgtgaacacc aggcgcatgg
     6121 ccgagcgact ggccatgcac ttgtcggaac gactgggcga gaacgcggtg accagccacc
     6181 acgggagcct gtcgaaggag acgcggctgg acgccgagcg ccggctcaag gccggcgagc
     6241 tgaaggcgct ggtcgccacg gcgtcactcg agctcggtat cgacatcggc accgtggatc
     6301 tggtctgcca ggttgcctcg acccggtcga tcgcgacgtt gctgcaacgg gtgggccgct
     6361 ccggtcacca cctcaaagcc atcccgaagg gccggctctt tccgctttcg cgagacgacc
     6421 tggccgagtg cgccgcgctc ctccgcgcgg tcaaatcc
//
LOCUS       DASUBP010000309         2886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_994582, whole genome shotgun sequence.
ACCESSION   DASUBP010000309 DASUBP010000000
VERSION     DASUBP010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994582"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="VFU75_11700"
     CDS             <1..286
                     /locus_tag="VFU75_11700"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HEU5467548.1"
                     /translation="VPVTAFVAVRYGSSGYVGDEPRMRSLLKHPGVLEAHHVAGDDCY
                     VLKVAARDLEGIQDILRTLAGSPKQLNTKTTIVLSTLFEKPSLTLPPEND"
     gene            288..1220
                     /locus_tag="VFU75_11705"
     CDS             288..1220
                     /locus_tag="VFU75_11705"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEU5467549.1"
                     /translation="MAGLRGRALVAYLLVCLFWGSTYLAIRIGVGVFPPFLFAGVRFF
                     IAGVILFTGALLFGDQLPGSRKDWIVLSVVGLFLLAGGNAMVVWAEQYTASGVASIFV
                     VTVALWMAFFDAVIPGGAGRISLRVIAGLLAGFAGTILLVGASPAALLHADLRGPIAL
                     TGASASWAFGSIFYKRQHPTASPYIAASIQMLAGGAVVTLLGLALGEGARVHVTATGL
                     EALFYLVIFGSLVGYSAYSYALKHAPATIVGTYAYVNPVIAVLLGWLLLGEPVSARTF
                     GAMALILGAVIWIQFSHALPAFRRAPTSAATARN"
     gene            1287..1586
                     /locus_tag="VFU75_11710"
     CDS             1287..1586
                     /locus_tag="VFU75_11710"
                     /inference="COORDINATES: protein motif:HMM:NF018045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bor family protein"
                     /protein_id="HEU5467550.1"
                     /translation="MRPIAAIACAALLVSTAACFHATIETGLAPSATVIEKPFASGWI
                     FGLIPPDPVDTKAKCGARGVSKVETQHSFVNMLVAFVTFDIYTPISITVTCAAGK"
     gene            1760..>2886
                     /locus_tag="VFU75_11715"
     CDS             1760..>2886
                     /locus_tag="VFU75_11715"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5467551.1"
                     /translation="MSEPAICSKCGTPIAPGQRFCAGCGADVSGQQGNVATAFVPKQD
                     MVRATMSQTALHDVVRHATIGEYEILAELGRGGMATVYLGHDIALDRKVAIKVMSPAL
                     LTGDGMVERFKREARTAASLSHPNIIPIYAVKEGDQIVYFVMKFVEGRPLDSIIKETG
                     KLPIQMVQQILQQVGAGLGYAHKRGIIHRDVKPANVMVDTEGWAVVTDFGIAKVTETK
                     GLTMTGATVGTPSYMSPEQCAAKELTGASDQYSLGVMAYEMLAGKLPFNADSIMAIMY
                     SHFNDPPPPIDEVRPDCPPEIVTAVMRMLAKEPAERFPTVEDAISAIGVPAMTPDDPV
                     RTQLMTLAASNKNTEMLKRLTTPVSMPGRTQAAKTTVSTTGG"
BASE COUNT          468 a          992 c          908 g          518 t
ORIGIN      
        1 ggttcccgtc accgcgttcg tcgcggtccg ctacggcagc tcgggctacg tcggcgacga
       61 gccgcgcatg cggtcactcc tcaagcatcc cggagtactc gaagctcacc acgtggccgg
      121 cgacgactgc tatgtcctca aagtcgccgc acgtgatctc gagggaatcc aggacatcct
      181 gcggacgcta gcggggtctc cgaaacagtt gaataccaag accaccatcg tgctcagcac
      241 gctgttcgag aagccgtcac ttacgttgcc tccggagaac gactgacatg gcaggcctcc
      301 gtggcagagc gctcgtggcg tacctgctcg tctgcctctt ctggggatcg acctacctgg
      361 cgatccggat cggcgtcggg gtctttccac cgtttttgtt cgccggcgtg cgcttcttca
      421 tcgccggcgt catcctgttc acgggggccc tcctcttcgg cgaccaactt cccggatcac
      481 ggaaggactg gatcgtgttg tcggtcgtcg ggctctttct gttggcgggc ggcaacgcga
      541 tggtcgtgtg ggccgagcag tacacggcct ccggggtggc gagcattttc gtggtgacgg
      601 tggccctgtg gatggcgttc ttcgatgccg tcatccccgg tggcgcaggc cgcatctcac
      661 tgcgcgtgat cgccggcctg ctggcgggtt tcgccgggac catcctgctg gtgggtgcga
      721 gtccggcggc gctcctgcac gccgacctcc gggggccgat cgcgttgacc ggggcgagtg
      781 cctcctgggc gttcggctcc atcttctaca agcggcaaca tcccaccgcg agtccgtaca
      841 tcgcggcgtc gattcagatg ctcgcggggg gcgccgtcgt caccttgctg ggactggccc
      901 tcggcgaagg cgcccgcgtg catgtcacgg cgacgggcct cgaagcgctc ttttatctcg
      961 tgattttcgg atccctggtt ggatacagcg cctactcgta tgccttgaag cacgccccgg
     1021 ccaccatcgt cgggacgtac gcctacgtga atcccgtgat cgccgtcctc ctgggctggc
     1081 ttctgctggg tgagcccgtc tcagcacgca ccttcggcgc gatggcgttg atcctgggcg
     1141 ccgtcatctg gatccaattt tcccacgccc tccctgcgtt ccggcgggcg cccacctcgg
     1201 cggcgaccgc tcgcaactga ccgatttgcg gggtcccccg gaggcgggca tattggcgcc
     1261 gactctcccc aaccaccgag ggacccatgc gtccgatcgc cgccatcgcc tgcgccgccc
     1321 tgctcgtgtc caccgccgcc tgcttccacg cgaccatcga aaccggcctg gcaccgtccg
     1381 ccaccgtgat cgaaaagccc ttcgcctcag gatggatctt cggcctgatc ccccccgacc
     1441 cggtcgacac caaggccaaa tgcggggccc gcggcgtctc caaggtcgag acccagcaca
     1501 gcttcgtgaa catgctggtg gcgttcgtga ccttcgacat ctacacgccc atctccatta
     1561 cggtgacctg cgccgcgggt aagtaacccc cccagactga agtctgggcc cggacatgca
     1621 gcgcgcttga agcgcggggt gtgcccgggc ccaccccttg gggtggcaag ggttactccc
     1681 ccctgacact gcccaagtcg ttgtggggcg gtatattatc tccccttatg tcacaccccc
     1741 cggccactcg tatgagccag tgagcgagcc cgcgatctgc agcaaatgcg gaacgcctat
     1801 cgcgcccggg caacgctttt gcgcgggctg cggcgccgac gtgtccgggc agcaagggaa
     1861 cgtcgcgacg gcgttcgttc cgaagcagga catggtccgc gccaccatga gccagaccgc
     1921 cctgcatgac gtcgtccggc acgcgacgat cggcgagtac gagatcctgg ccgagctggg
     1981 ccggggaggc atggcgacgg tgtacctcgg gcacgacatc gcgctcgacc gcaaggtcgc
     2041 gatcaaggtg atgtcccccg ccctgctcac tggcgatggc atggtcgagc gcttcaagcg
     2101 cgaggcccgt accgcggcgt ccctctcgca tccgaacatc atcccgatct acgccgtcaa
     2161 agagggcgat cagatcgtct acttcgtgat gaagttcgtc gaaggtcgcc cgctggattc
     2221 catcatcaag gagacgggca agctcccgat ccagatggtt cagcagattc tccagcaagt
     2281 cggcgcggga ctcggctacg cgcacaagcg cgggatcatt catcgcgacg tgaagcccgc
     2341 caacgtcatg gtggacaccg agggctgggc cgtcgtcacc gacttcggca tcgccaaggt
     2401 caccgagacc aagggcctca ccatgacggg cgccacggtc ggcacgccgt cgtacatgag
     2461 cccggagcag tgcgccgcca aggagctgac gggcgcgtcg gatcagtatt cgctgggagt
     2521 gatggcgtac gagatgctcg ccgggaagct gccgttcaac gcggattcca tcatggcgat
     2581 catgtactcg cacttcaacg atccaccgcc gcccatcgat gaggttcggc ccgactgccc
     2641 gccggaaatc gtgacggcgg tcatgcggat gctcgccaag gaacccgccg agcgcttccc
     2701 caccgtcgaa gacgccatct cggccatcgg cgtaccggcg atgacgcccg acgatccggt
     2761 gcgcacgcag ctgatgacgc tggcggcgtc gaacaagaac accgagatgc tgaagcgcct
     2821 gacgacgccg gtgtcgatgc ccgggcggac gcaagccgcg aagacgacgg tgtccaccac
     2881 cggcgg
//
LOCUS       DASUBP010000310        16217 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1034819, whole genome shotgun sequence.
ACCESSION   DASUBP010000310 DASUBP010000000
VERSION     DASUBP010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 16217)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16217)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16217
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034819"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VFU75_11720"
     CDS             <1..481
                     /locus_tag="VFU75_11720"
                     /inference="COORDINATES: protein motif:HMM:NF021235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HEU5467552.1"
                     /translation="HHAPTAETAAKTCASCHSRSDCVQCHSPDAASAGAYHPAGFLSR
                     HPAAAYARESSCNTCHNPGQFCVSCHKSAGLVATAALGKGYHDASRFFVSGHGVAARQ
                     SLETCTSCHVERDCLTCHSALGGRRFDPHGPGFDAARMIKKNPEVCTACHGTAIPTR"
     gene            579..1046
                     /locus_tag="VFU75_11725"
     CDS             579..1046
                     /locus_tag="VFU75_11725"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEU5467553.1"
                     /translation="MFSITKRSPDPMLDLLYRLEQRMGRALEPFRSFDWEVGEPSTAW
                     VPAVDIVEEPDLMKITAEIPGVKPEDVKVTVTDNLLTISGTKMHVKEEKTSKTHRFER
                     EYGAFQRTFTLPTTVNGKGIKAAYGEGLLTVTLPKVETAKPQSVSIDVVPTHK"
     gene            1146..1871
                     /locus_tag="VFU75_11730"
     CDS             1146..1871
                     /locus_tag="VFU75_11730"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HEU5467554.1"
                     /translation="MNCSSCGKPLPDSAQFCHHCGARAGAQTASRGWQAAAPWALAGF
                     FVGLLVGVVADGIFRRPATPAASDATASATAGAPFAGSGGGGAASVDIASMTPQERAS
                     RLYTRVMSLHAAGKPDSAEFFLPMALQAYGMLPEVTPDAHYHIGVLQLTSGNMPAALA
                     EADTIQKQVPTHLFADMLRARVYGLRRDTKSYEAACRSYLKHESAELAKKRPEYTEHA
                     GTIAAFHEEATGIAKGTGGSPAS"
     gene            complement(1873..2397)
                     /locus_tag="VFU75_11735"
     CDS             complement(1873..2397)
                     /locus_tag="VFU75_11735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467555.1"
                     /translation="MAPRAGVYIVPIDKWYIERTVWLIAGVVLLTSTALALWVHPLFV
                     FGVIATGLASIAVAFTGFCIVGNVLYRLGFKPKLGRPGMSRWYFMQTDAWYLERRIYL
                     AVGINISVASTLSLTWTPWWLAFTAFVGGAMVWFASTGFCIMANGLYWLGAEPRLSPL
                     TKQGECAVERPIAA"
     gene            2537..4363
                     /locus_tag="VFU75_11740"
     CDS             2537..4363
                     /locus_tag="VFU75_11740"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEU5467556.1"
                     /translation="MRTFVRQNPLVLLSAVGLAAGVAAHLGFGAKSVANWCWLVVIIG
                     AGTPLLWKTFRGLLGGVFAADVVAALAIITAVVVGQYFAGAVVVLMQSGGEALERHAF
                     ARASDSLEALLARAPRIAHRQGATGIEDVPVEVVTPGDRLLIRPGDLVPVDATVLEGT
                     SAVDQSALTGEPIPLRAAPGTTLMSGSINLDGALVARADRASNQSQYQRIVQLVESAR
                     RERAPLERLADRYAAWFTPVTLLMCGAAWLITRNPDSVLAVLVVATPCPLILATPVAV
                     IGAISRVAEHGVIVKTGAAIEQLGRADVFVFDKTGTLTLGHPTVRNVRAQPGYDAKRV
                     LRLAGAVEQHSSHHLGRAVTEAAQAHGESLPAVTAVREWPGSGVAGTVEGHTVLVGSP
                     ALIAERGIVVPDLDGEQTTACVVVDAHLAGTIEFADRLRHQVPALMQRLVVLGVNETV
                     MLTGDRESAAQVIAAQAGIRTVRAGLLPDQKVAEVLALKERDHRVVVMVGDGINDAPA
                     LATATVGIALGAHGVAASAEAADVVVLVDDIALVGDAMAVSQRMRRIALQSIVTGLGV
                     SGALMAVAATGHIPPAAGALLQEALDVAVILNALRVRLSPRP"
     gene            complement(4347..5003)
                     /locus_tag="VFU75_11745"
     CDS             complement(4347..5003)
                     /locus_tag="VFU75_11745"
                     /inference="COORDINATES: protein motif:HMM:NF016836.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BON domain-containing protein"
                     /protein_id="HEU5467557.1"
                     /translation="MDRTPTFLEQVQEELAWEPSVYAGSITAATVDGIVTLTGTVRTF
                     AEKVAAERAVRRVRGVRGVVNDLDVKLLVTHRHPDTEIAKAAVHALQWNSVVPRDAIR
                     VHVSDGWVRLEGAVDAQFQRAAAEEAVRTLVGVLGVSNRVTLRPPAQIPQVRAKIEAA
                     LHRRASLDAAKVQVRTRDDHTILLSGSVSSWQERELVEAAAWAAPGVIAVEDEIKVEG
                     "
     gene            complement(5003..5809)
                     /locus_tag="VFU75_11750"
     CDS             complement(5003..5809)
                     /locus_tag="VFU75_11750"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467558.1"
                     /translation="MTTALRLEPRTRSAPFRVLAVVLDRAHARVFAVTPTTVAEIADI
                     ESPATRGGRFHSDRHGAPGGGERAFHQRLEEESRRHFLDVIRVLTTALVGHPDDEVFL
                     AGPGPYATVFERHIPVDLKHRIIGTEQMNPLDATPAVVAAVVRDAKRHHQHQAEQAVM
                     AAVAEGVGTGMATNGVRETLHALGHGQVRALIIHPHARCTGFRCLKTGRLAMSRWDYP
                     GEGPAREVADVIRAAAREAVRQRADVILIQDPTVAREVDGLAALLRFKEG"
     gene            5934..6326
                     /locus_tag="VFU75_11755"
     CDS             5934..6326
                     /locus_tag="VFU75_11755"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEU5467559.1"
                     /translation="MHVAELMRTNLKTVAAEATVADAVCLLADARVSALPVVDRVGRA
                     VGILTTRNVLEAERDARDRNRLLQHTLVLEIMTPWAATIGPDVDVQEAARQMLYLDVQ
                     RLFVEEDGKLVGVISQTDIVGAVATAGV"
     gene            6467..7681
                     /locus_tag="VFU75_11760"
     CDS             6467..7681
                     /locus_tag="VFU75_11760"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HEU5467560.1"
                     /translation="MRDRFERLLSAGVEIFSQHTLTAVLQQVVDAAREVVGARYAALG
                     VLAPGGTSLSEFVTSGMTVEEREKIGALPIGHGLLGVVIRRPKPIRVPDISKHSQSAG
                     FPKHHPAMRSFLGVPIVGRGNKVFGNLYMTEKTGAAEFSEEDEAIGILLAGSAAVAIE
                     NAALNEETQSLLAQVQNMQRQRDLFFAMMNHELRNALTGVFGWAEMLVRKRDGAPVSK
                     EAREVYEGAERTIELLNNFLDLTRLDSGRVRPVFREVDPAKCVERACASVRLAAEAKQ
                     VRLATDCPATLPPPTTDQVRVQQILMNLLSNAVRHTATGSTVTTAVRTTPGHIEFRVS
                     DHGPGVPEAVREGIFEPFRRFDPHSGQGTGLGLPVSRRLAEILGGTLGVERSDSNGAT
                     FLLRLPLIPPDL"
     gene            7763..8449
                     /locus_tag="VFU75_11765"
     CDS             7763..8449
                     /locus_tag="VFU75_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEU5467561.1"
                     /translation="MKLLVVEDDRKVAGFIEQGLREEGYAVDVAPDGEQATMLAHVNE
                     YDLILLDLMLPKKNGLQVASELRGEGRSTPVLMLTARDASEDVVRGLDAGADDYLTKP
                     FKFDELLARVRALVRRGGAGRTELLSYGPVTMDRLKHRVQIQGKKLELTPKEFQLLEH
                     FLLHPEAVQRRTELLEKVWDMHFDPESNVVDVHVGNLRRKLHEAAGTELIRTVRGVGF
                     ILQNGNGDDN"
     gene            8499..8636
                     /locus_tag="VFU75_11770"
     CDS             8499..8636
                     /locus_tag="VFU75_11770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467562.1"
                     /translation="MPDQPPESPHPKGTLAIIGLYGLLFALGWFAMYVFVYLHRGPVT
                     P"
     gene            8633..9109
                     /locus_tag="VFU75_11775"
     CDS             8633..9109
                     /locus_tag="VFU75_11775"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEU5467563.1"
                     /translation="MKVDFYEKIWLWAAVVIMVAFATTVVVGAVAEGYAPPSHVETIN
                     PATVLSDPRFATRGVTVDTARVTVTAVGMTFAFLPSEIHVPAHRPVTFRLTSIDVTHG
                     FEIVGTNGNTMLIPGYVSQFTTTFDTPGEYLIVCNEYCGQGHHTMSGKLIVEEARK"
     gene            9106..10731
                     /locus_tag="VFU75_11780"
     CDS             9106..10731
                     /locus_tag="VFU75_11780"
                     /inference="COORDINATES: protein motif:HMM:NF012343.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HEU5467564.1"
                     /translation="MTRDDRLSVAHLWVAISAFGIAACMALMQALSRANLDLPWRSAK
                     MYYLSVTAHGVLMALVFTTFFIMGFGYVTISRSLNRPVSWYRLAWSGFWIALTGTLLT
                     AGTILAGKASVLYTFYPPLKAYPTFYIGAALLVIGSWCWVWSLFASYRDWRREHPADK
                     TPLPFHAMMATAIVWQIATVGVAIEVVVLLIPWSLGWTPTIDPLLARMLFWYFGHPLV
                     YFWLLPAYAVWYTVFPRVAGGQLFSEPLGRLTFALFIVLSTPVGFHHEALDPGIPAGW
                     KLFHAVLTFGILFPSMVTAFTITASMELAGRARGGTGWFGWLRKLPWTDPLFASVALS
                     MLLFAVGGFGGAINASFGMNAVVHNTAWIQGHFHLTVGSATALTFMGTAYWLMPRLLG
                     KNLELGLLAKVQPYLWFLGMMLFSFSNHITGLMGMPRRIYDASYGGSPVAEGWKVLTG
                     VSAAGGLLLFVSAAFFVMVMVGTGLAGKQRAFAEPILWAEPLEDPGPGRTIFDRYGMW
                     VIAAILIILIAYAIPLAQHLSMPTFGSPGVPQY"
     gene            complement(10815..11240)
                     /locus_tag="VFU75_11785"
     CDS             complement(10815..11240)
                     /locus_tag="VFU75_11785"
                     /inference="COORDINATES: protein motif:HMM:NF012272.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005344 - oxygen carrier activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="globin"
                     /protein_id="HEU5467565.1"
                     /translation="MTVPQASIDSATLDAAHASYERASGTPEFYASFYANFFKVCPDA
                     RPRFAKTDFDRQHKLLHHAIGMLLIYPKHLNDEPNLLSRLAKRHSRKDLDIPASMYRP
                     FVDALITTLRQCDPSFTGPLETGWRAILDVGIRYMQSKY"
     gene            complement(11237..12751)
                     /gene="glpK"
                     /locus_tag="VFU75_11790"
     CDS             complement(11237..12751)
                     /gene="glpK"
                     /locus_tag="VFU75_11790"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEU5467566.1"
                     /translation="MKSVLAIDEGTTGARALVIADDGRVLGRGYREIPQHFPRPGWVE
                     HDPEDLWAASLDACREALAQAKTKPLAIGITNQRETVCVWDRKTGKPLHRAIVWQDRR
                     TADRCAALAGTPEGKAIKERTGLVLDPYFSGTKIEWLLNQVPGVRGQVSGGDAVFGTV
                     DSWLLHRLTGGRVFATDHTNASRTLLYNIGELGWDDGLLKIFGVPRHALAEVRASSGD
                     FGVAVADHFGFEIPVLGIAGDQQAALYGQGCWTAGQAKNTYGTGAFLLMNTGGQPAHS
                     EQGLLTTVGCDARGRPAYALEGSVFIAGAAVQWLRDGLQIIERAEETEALAKSVPDTG
                     GVSFVPAFVGLGAPHWNPAARGTIVGLTRGSTRAHLVRAALEAMAYSTKDVLDAMTAD
                     AHLSLSALQVDGGASANGWLMQFQADVLNVPVARPDLVETTSLGAAGLAGIAGGVWAR
                     PEEFLASRTFTRFSPGIGGAAARARVPEWHRAVDAALAWARGGPIFPGTSKEPA"
     gene            12808..13590
                     /locus_tag="VFU75_11795"
     CDS             12808..13590
                     /locus_tag="VFU75_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HEU5467567.1"
                     /translation="MTYQTLLFDVKDGVAVVTINRPDKLNALNDQVMAELADAAERIA
                     TDPAIKGAILTGAGPKSFVAGADISDLAKQGPFDGKARAMRGQGVLRRLETCGKPVIA
                     AVNGFALGGGCELAMACHIRIASENAKFGQPEVKLGIAPGYGGTQRLPRLVGKGVALQ
                     LILTAEMIDAAEAYRIGLVNKVVPANELMAETEKMLRGILSMGPLAVRLSMEAIDHGL
                     EMTLDEGLLLEANHFGLLAATQDTKEGLSAFLEKRPAKFTGK"
     gene            13602..13940
                     /locus_tag="VFU75_11800"
     CDS             13602..13940
                     /locus_tag="VFU75_11800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467568.1"
                     /translation="MLAAGALACGEDRPRPGPPDLSIQFQRDSLHSPDTLRGTIQATD
                     NEGIDSVWLNVGTKDSAAADGGFQFSFSAPFLFFIDSGLIKGAKIPVEFRARDIAGYS
                     DVLDTFVVIK"
     gene            14043..14354
                     /locus_tag="VFU75_11805"
     CDS             14043..14354
                     /locus_tag="VFU75_11805"
                     /inference="COORDINATES: protein motif:HMM:NF017103.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HEU5467569.1"
                     /translation="MAVSEVLSGYLKRSGLEGRLAQARIIPDWATLVGAQVAAVTMPS
                     RVTPDGTLFVGVQTSPWMNELMLMTPTIIARLNAGRGDGRIKGIRWFLLTERDHGEGK
                     A"
     gene            14335..>16217
                     /gene="gyrB"
                     /locus_tag="VFU75_11810"
     CDS             14335..>16217
                     /gene="gyrB"
                     /locus_tag="VFU75_11810"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEU5467570.1"
                     /translation="MAKAKPDPKQSNNGGYQADSIQVLKGLEAVRRRPAMYIGSVSAR
                     GLHHLVYEVVDNSVDEALAGYCTQIDVSIHSDNSITVADNGRGIPVDIHKTEKKPGVE
                     VALTVLHAGGKFDKNTYKVSGGLHGVGVSVVNALSEKLEVWVERDGKKYHMAFAKGDT
                     TKKLEVVGKAKTTGTTVWFKPDETIFTETRFDYATLANRLRELAFLNKGLTITLKDER
                     KGGKTETYFYKGGLTEFVKWLLGNRKPLHQKVITFEANKDGVEVEGAVQYDDQYGENT
                     FTFVNNINTHEGGTHLTGFKSALTRTINEWAKKDGLLKKEEFTLSGDDVREGLTSVIH
                     VKVKDPQFEGQTKTKLGNSEVEGIVKSVVNEALGDFFDKSPSVARAIIAKAISAARAR
                     EAARKARELVRRKNAMEGGLLPGKLADCSLTDPKLCEMYLVEGDSAGGSAKQGRDRSF
                     QAILPLRGKILNVQKARIDKILSNEEIRTMITAIGTGVGDDFKIEDARYHKIIIMTDA
                     DVDGAHIRTLLLTFFYKEMKALLDAGYIYIAQPPLYRVAKGKEEFYAYDEKERDGYAK
                     RLGGGGEEVKSNVMVQRYKGLGEMNPDQLWKTTMDPAARTILKVTMEDALEASQLFET
                     LMG"
BASE COUNT         2867 a         5435 c         5203 g         2712 t
ORIGIN      
        1 gcatcatgcg cccaccgccg aaaccgcggc gaagacgtgc gccagctgtc acagccggag
       61 cgactgcgtg cagtgccaca gccccgacgc cgccagcgcc ggggcttacc atcccgccgg
      121 attcctgagc cggcatcccg cggccgcgta cgcccgggag tcgtcgtgca acacgtgcca
      181 caacccgggc cagttctgcg tctcgtgcca caagagcgcg ggcctcgtgg cgaccgcggc
      241 gctcggcaag ggctaccacg acgcctcgcg attcttcgtt tccggccacg gggtcgcggc
      301 gcgccagagc ctcgagacct gcacaagctg ccacgtcgag cgcgattgcc tgacctgtca
      361 ttccgcgctg ggcgggaggc gcttcgatcc gcacgggccg ggattcgacg ccgcgcgaat
      421 gatcaagaag aatcccgagg tctgcaccgc gtgccacggc acggcgatcc cgacccgttg
      481 atgatgaagg tggttgaacg gaatgctcaa gcgcgattca cgaccgtttc aggcctggcg
      541 ttttattctc cgctcgtact ccaacggagg caaacgccat gttctcgatc acgaagcgct
      601 cacccgaccc catgctcgac ctgctctatc ggctggagca gcgcatggga cgtgcgctcg
      661 aaccgttccg ttccttcgac tgggaagtcg gtgagccgtc gacggcctgg gtgccggcgg
      721 tggacatcgt cgaagagccg gacctcatga agattacggc cgagatcccc ggagtgaagc
      781 cggaggacgt gaaggtgacg gtcaccgaca acctgctgac gatcagcggg acgaagatgc
      841 atgtgaagga agagaagacg tcgaagacgc atcggttcga gcgggaatac ggtgcgttcc
      901 aacgcacctt cacccttccc accacggtga acgggaaggg catcaaagcc gcctatggcg
      961 aaggcctgct cacggtcacg ctgccgaaag tcgagaccgc gaagccgcag agtgtgtcga
     1021 tcgacgtcgt gccgacgcac aagtagtcga acccgcgatt gcgcgacgtc cacctcctcc
     1081 cggacaccac ctgggaggag gtatgttttc gcgcataaag gacgttcccc ccttggaggc
     1141 acctgatgaa ctgctcgtcc tgtggtaagc ccctgcccga tagcgctcag ttctgccacc
     1201 actgcggcgc ccgcgccgga gcacagaccg cctcccgagg gtggcaggcc gcggccccgt
     1261 gggcgttggc gggattcttc gtcggtctcc tggtgggagt ggtggcggac ggcatcttcc
     1321 gaaggccggc aacccccgcc gcctcagacg caaccgccag cgcgacggcc ggcgcgccct
     1381 tcgccggcag tggaggcggt ggagcggcat ccgttgacat cgccagcatg accccgcagg
     1441 agcgcgccag ccgcctgtac acccgggtaa tgagtctgca cgccgccggg aagcccgact
     1501 ccgccgaatt ctttctcccg atggcgctgc aggcctacgg catgcttccg gaggtgacgc
     1561 cggacgccca ctaccacatc ggcgttctcc aactgacttc agggaacatg cccgccgccc
     1621 tggccgaggc ggacaccatt cagaagcaag tgcctaccca tctgttcgcg gatatgctgc
     1681 gggcccgggt ctacggcctc aggcgggaca cgaagagcta cgaagccgcc tgccggagct
     1741 atctcaagca cgagtcagcg gagctcgcca agaagcgccc cgagtacacc gagcacgccg
     1801 gtaccatcgc ggcctttcac gaagaagcga ccgggatcgc caaggggacc ggtggttccc
     1861 ccgcaagctg atctaggcgg cgatcggccg ctccaccgcg cactcgccct gcttggtcaa
     1921 gggcgagagg cgcggttccg cacctagcca gtagaggccg ttggccatga tgcagaaccc
     1981 ggtggaggcg aaccacacca tggcgccacc cacaaacgcc gtgaacgcaa gccaccatgg
     2041 cgtccaagtc agcgaaaggg tcgacgccac cgagatgttg atccccaccg ccaggtagat
     2101 ccgccgctcg agataccagg cgtccgtctg catgaagtac caccgggaca tccccggccg
     2161 gcccagcttg ggcttgaagc ccaaccggta cagtacgttc cccacgatgc agaagcccgt
     2221 gaaggcgacg gcgatcgacg ccaagccggt ggcgatcacg ccgaatacga acaacggatg
     2281 cacccagagt gcgagtgcgg tgctcgtcaa caggaccaca ccggcgatga gccagaccgt
     2341 gcgttcgatg taccatttgt cgatcgggac tatgtagacg ccggcgcggg gagccatcgg
     2401 aatgcctcga atggagttct tccaacatgt cccccgggcc gggccgtggg gaagacaccg
     2461 agtatgaaga tcccttcatg gatcgttcct gccggtttca atccccgtga ataccctgac
     2521 cattatgagt ggctcgatgc gcaccttcgt gcggcagaat cccctcgtct tgttgtccgc
     2581 ggtggggctc gccgcgggcg ttgcagcgca cctcggattc ggcgccaagt ccgtcgccaa
     2641 ctggtgctgg ctggtggtga tcatcggcgc cgggaccccg ctgctctgga agacgtttcg
     2701 gggcctcctg ggaggggtgt tcgccgccga cgtggtggcc gcactggcca tcatcaccgc
     2761 agtggtcgtc ggccagtact tcgccggagc ggtggtcgtc ctgatgcagt cgggtggcga
     2821 agccctggag cgccacgcgt tcgcccgcgc gtcggactcg ctcgaagcac tgctcgcccg
     2881 ggctccccgc atcgcgcatc gccaaggcgc cacgggaatc gaagacgttc cggtggaggt
     2941 ggtgaccccg ggagatcggc tcctgatccg ccccggagat ctggttcccg tggacgccac
     3001 ggtcctcgag ggaacctccg cggtcgacca gtccgccctc accggcgaac ccatccccct
     3061 gcgagccgcg cccggcacga cgctcatgtc ggggagcatc aacctcgacg gcgcactggt
     3121 cgcgcgcgcc gaccgtgcca gcaatcagag ccagtatcag cgcatcgttc agctggtgga
     3181 gagcgcccga cgggaacggg ctcccctcga gcgtctggct gaccgctacg ccgcgtggtt
     3241 cactcccgtg accctcctca tgtgcggcgc cgcgtggctg atcactcgga atccggacag
     3301 cgtcctcgcc gtcctggtcg tcgcgacgcc ctgtccgctc attctcgcca cgcccgtggc
     3361 ggtgatcggc gccatcagcc gcgtggccga gcacggggtc atcgtgaaga cgggtgcggc
     3421 catcgagcag ctgggacgag cagatgtctt cgtgttcgac aagaccggca ccctcaccct
     3481 ggggcacccc accgtccgaa acgtgcgcgc ccaaccgggc tatgacgcga agcgcgtgct
     3541 gcgtttggcg ggcgccgtgg agcagcactc ctctcaccat ctcggacggg ccgtgacgga
     3601 agcagctcag gcgcacgggg agtccttgcc ggccgtgacg gcggtgcggg agtggccggg
     3661 atccggggtc gccgggaccg tggaaggcca cacggtgctg gtcgggtccc ccgccctgat
     3721 cgcggagcgc ggcatcgtgg tgccggacct ggacggtgag caaaccaccg cgtgcgtcgt
     3781 cgtggacgca cacctggccg gcaccatcga atttgcggac cgtctccggc accaggtgcc
     3841 ggcgctgatg cagcggttgg tcgtcctggg agtgaatgaa acggtgatgc tgaccgggga
     3901 ccgggaatcc gcggcgcagg tgatcgcggc gcaggccggg atccggaccg tccgcgccgg
     3961 gctactcccc gatcagaaag tggccgaggt ccttgccctg aaggaacggg atcatcgggt
     4021 ggtcgtcatg gtcggagacg gcatcaacga cgctccggcg ctcgccacgg cgacggtcgg
     4081 gatcgccctc ggcgctcatg gcgtcgccgc atccgccgaa gcggccgatg tggtggtgct
     4141 ggtcgacgat atcgccctgg tgggggacgc catggccgtc agtcagcgga tgcggcggat
     4201 cgccttgcag agcatcgtga ccgggttggg agtgtcgggc gccctgatgg cggtggcggc
     4261 gaccggtcat atcccgcccg ccgccggggc gcttctgcag gaagcactcg atgtcgccgt
     4321 gatcctgaat gcccttcgcg tccgcctcag ccctcgacct tgatctcatc ctcgaccgcg
     4381 atgacgcccg gggccgccca ggccgccgct tccacgagct cccgttcctg ccacgaggat
     4441 accgacccgc tcagcaggat cgtgtgatcg tcgcgggtcc ggacttgaac cttggcggcg
     4501 tcgaggctcg cgcgacgatg cagcgccgct tcgatcttcg cccgcacctg cgggatctgc
     4561 gccggtgggc gcagcgtgac gcggttggat acgcccagca ctcccaccag cgtccggact
     4621 gcctcctcgg cggccgcgcg ttggaactgc gcgtcgacgg cgccttcgag ccggacccac
     4681 ccatccgaca catggacccg gatcgcatcg cgcggtacca ccgaattcca ttgcagggcg
     4741 tggacggcgg ccttcgcgat ctcggtatcg ggatggcgat gggtcaccag gagctttaca
     4801 tcgaggtcgt tcaccacacc ccgcacaccg cgcacacggc gaaccgcgcg ttccgccgcc
     4861 accttctcgg cgaaggtgcg caccgtgccc gtcagcgtca cgatgccatc caccgtggcc
     4921 gcggtgatcg agccggcgta gacactcggc tcccaggcca gctcctcctg cacctgctcc
     4981 aagaacgtcg gcgtcctgtc catcacccct ccttgaaccg cagcagcgcc gccaatccgt
     5041 cgacttcccg tgccaccgtg gggtcctgaa taagaatcac gtccgcgcgc tgccggacgg
     5101 cctcccgggc cgcggcgcgg atcacgtcgg cgacttcacg ggcgggacct tcacccggat
     5161 agtcccaccg ggacatcgcg agccggcccg tcttcaagca ccggaagccc gtgcaacggg
     5221 cgtgcgggtg tatgatcagc gcacgcacct gtccgtgccc cagggcgtgc agcgtttccc
     5281 gcacgccgtt ggtcgccatt cccgtcccca cgccttcagc caccgccgcc atcacggctt
     5341 gctccgcctg atgctggtgg tggcgcttgg catcgcggac caccgcggcc acgaccgccg
     5401 gcgtggcgtc caacggattc atctgctcgg tcccgatgat gcgatgcttc aggtccaccg
     5461 ggatgtgacg ctcgaatacc gtcgcgtagg ggcccggtcc ggccagaaag acttcatcgt
     5521 cgggatgccc gacgagtgcc gtggtcagca cccgaattac gtccaggaag tggcgccggg
     5581 attcctcctc gaggcgctgg tggaaagccc gctctccccc cccgggcgcg ccatgccggt
     5641 cactgtggaa ccggccaccg cgggtggccg gggactcgat atccgcgatc tccgcgaccg
     5701 tggtcggagt caccgcgaaa accctcgcgt gggcccggtc cagcaccacg gcgaggaccc
     5761 ggaacggagc ggagcgcgtg cgcggctcaa gtcgaagtgc agtcgtcatg ggggggaagg
     5821 tgttctcgcg gctctgaaga gagcgtgaaa acacccagaa ggctcaggcg cctttcagga
     5881 catcttcatc caggctgagg caccttcccg gcaacaccat cggcggagga tatatgcacg
     5941 tcgcggaact catgcggacc aacttgaaga cggtggcggc ggaagcaacg gtggccgacg
     6001 ccgtgtgtct cctggccgat gcccgcgtct cggcactgcc ggtcgtggat cgcgtcggtc
     6061 gggccgtggg tatcctcacc acgcgcaacg tgctggaagc cgagcgggac gcgcgagacc
     6121 gcaatcgctt gctccagcac accctggtcc tcgagatcat gaccccatgg gcggccacca
     6181 tcggcccgga tgtcgatgtc caggaagccg cgcgccagat gctctatctc gacgtgcagc
     6241 gcctcttcgt cgaagaggat gggaaactgg taggcgtgat ctcgcaaacg gacatcgtag
     6301 gagccgtcgc gacggcgggg gtatagcgca aaagcatgga gagagggccc gggaccggcc
     6361 ctagccgttc tgcccgggcc tgccgcatta ttaaggaact atgaagaagc ccccggggcc
     6421 aaaggccccc acccgagcgg gcactcatag caacgacgcg gcccgggtgc gtgatcgctt
     6481 tgagcgtttg ctctccgccg gcgtcgagat cttctcccaa cacaccctga ccgccgttct
     6541 gcaacaggtt gtcgatgctg ctcgcgaggt cgtcggagcc cgttacgcgg ctttgggggt
     6601 gctcgcaccc ggcggcacgt cactctctga attcgtgact tccggcatga cggtggaaga
     6661 gcgagagaag atcggggcgc tgccgatcgg tcacggcctg ctcggcgtgg tcatccgtcg
     6721 ccccaagccg atccgcgttc ccgacatctc caagcactcc cagtctgccg ggtttccgaa
     6781 acatcacccc gcgatgcgct cattcctcgg cgtgccgatc gtgggccgcg ggaacaaggt
     6841 cttcggcaac ctgtacatga ccgagaagac cggcgccgct gaattcagtg aggaagacga
     6901 agccatcggc atcttgctcg cgggttccgc cgccgtggct attgaaaacg ccgcgctgaa
     6961 cgaggaaacc cagagcctgc tggcgcaggt gcagaacatg cagcggcaac gtgacctgtt
     7021 cttcgcgatg atgaaccacg agctccgcaa cgcgctcacc ggcgtgttcg gctgggcgga
     7081 gatgctggtt cggaagcggg acggcgcccc cgtttcgaag gaagcgcgcg aagtgtacga
     7141 aggtgccgaa cggaccatcg agctcctcaa caacttcctc gatctcacgc ggctcgacag
     7201 cgggcgcgtg cgccccgtgt tccgcgaggt cgacccggct aaatgtgtgg agcgcgcgtg
     7261 tgcctccgtg cggctggccg ccgaggcgaa acaggtcagg ctggctaccg actgtcccgc
     7321 gaccctcccg cctcccacca cggaccaggt gcgagtgcag cagatcctga tgaatctgtt
     7381 gtcgaatgcg gtgcggcata cggcgaccgg aagcacggta accacggcgg tccggacgac
     7441 gcccgggcat atcgaatttc gggtgtcgga ccacggaccc ggtgttccgg aagcagttcg
     7501 ggagggcatc ttcgagccct ttcggcgctt cgatccccac tccggtcagg ggaccggcct
     7561 gggcctcccg gtatcccggc ggctggcgga gattctgggt ggaacgttgg gagtcgagcg
     7621 ctcggactcg aacggcgcca ccttcctgct ccgcctcccc ctgattcccc ccgatttatg
     7681 aagatcccct catgatgtct ttagaatcgg ctcactagtg cccgaataag cttgccttcg
     7741 acacttcttc atagagggtg tgatgaagct gctcgtggtg gaggacgacc ggaaggtcgc
     7801 ggggttcatc gagcaaggcc tgcgggaaga ggggtacgcc gtggatgtgg cccccgacgg
     7861 tgagcaggca accatgcttg cccacgtcaa cgaatacgac ctcatcctgc tcgacctgat
     7921 gctccccaag aaaaacggtc tgcaggtggc gagtgagctt cggggggagg gccggtcgac
     7981 gccggtattg atgctcaccg cgcgcgacgc gtctgaagac gtggtccgtg gcctcgacgc
     8041 gggtgccgac gactacctga ccaagccgtt caagttcgac gagctcctgg cgcgagtgcg
     8101 ggcactggta cgccgcggag gcgcgggccg cacggagctc ctgagctatg gccccgtcac
     8161 gatggaccgt ttgaaacatc gggtccagat tcagggcaag aagctggaac tcaccccgaa
     8221 ggaattccag ctcctcgagc atttcctgct gcacccggaa gccgtgcagc ggcggaccga
     8281 gctgctcgag aaggtctggg acatgcactt cgaccccgaa agcaacgtcg ttgacgtgca
     8341 cgtcgggaat ctccgccgca agcttcacga ggctgccggc acggagttga tccgcacggt
     8401 gcggggtgtg ggcttcattc tgcagaacgg aaatggtgac gacaactgac tgactgactg
     8461 actgactgac tgatccccaa ccttcgagga ggcccttcat gccggaccag ccgccggaga
     8521 gcccccatcc caaaggtacg ctcgcgatca tcggcctcta tgggctgctc ttcgcgctgg
     8581 ggtggttcgc gatgtacgtc ttcgtctacc tccatcgggg accggtgacc ccatgaaggt
     8641 cgacttctac gagaagatct ggctgtgggc cgccgtggtg atcatggtgg ccttcgcgac
     8701 cacggtcgta gtcggcgccg tcgccgaagg atacgccccc cccagccacg tcgagaccat
     8761 caatcccgcc acggtgctgt ccgacccccg cttcgccacc cgcggcgtga cggtggacac
     8821 ggcccgcgtg accgtgacgg ccgttggcat gaccttcgcc ttcctgccct cggagatcca
     8881 cgttccggct caccgcccgg tcacgttccg actgacctcg atcgacgtta cgcacgggtt
     8941 cgaaatcgtg ggaaccaacg ggaataccat gctcattccc ggatacgtca gtcagttcac
     9001 gaccaccttc gacacgccgg gcgagtactt gatcgtctgc aacgagtatt gcggccaggg
     9061 acatcacacc atgtccggga aactgatcgt cgaggaggcc aggaaatgac ccgcgacgac
     9121 cggctctccg tcgcccacct gtgggtggcc atttccgcgt tcgggatcgc cgcctgcatg
     9181 gcgctgatgc aggccttgtc gcgcgccaac ctcgatctcc cttggcgctc ggcgaagatg
     9241 tactacctgt cggtcacggc ccatggcgtc ctcatggcgc tggtgttcac gaccttcttc
     9301 atcatgggat tcggatacgt cacgatatcc cggtccctga accggcccgt ctcctggtac
     9361 cggctcgcct ggagcgggtt ctggatcgcc ctcaccggca cgttgctcac ggcggggacc
     9421 atcctggccg gcaaggcgtc ggtcctgtac acgttctacc cgcccctcaa ggcctacccg
     9481 acgttctata tcggcgcggc ccttctggtg atcggttcgt ggtgctgggt gtggagcctc
     9541 ttcgcgagct accgcgattg gcgccgcgag catccggccg acaagacgcc cctgcccttc
     9601 cacgccatga tggctacggc catcgtttgg caaatcgcca cggtcggtgt cgcgatcgaa
     9661 gtcgtggtgc tcttgatccc ctggtccctg ggctggacgc cgaccataga tccgctgctc
     9721 gcccggatgc tgttctggta cttcgggcac ccgctggtct acttctggct gctccccgcc
     9781 tatgccgtct ggtacacggt cttcccgcgc gtcgcgggcg gccagctgtt cagcgagcct
     9841 ctcggccgcc tcacctttgc gctcttcatc gtcctgtcca cgcccgtcgg cttccaccac
     9901 gaggcgctgg atccgggcat cccggcgggg tggaagctct tccacgccgt gctcaccttt
     9961 ggaatcctgt tccccagcat ggtgaccgcg ttcaccatca cggcgtccat ggagctggcc
    10021 ggaagggcgc gtggcggaac ggggtggttc ggctggctcc ggaagctccc atggactgac
    10081 ccgctctttg caagcgtggc gctgtcgatg ttgctcttcg cggtcggcgg cttcggaggc
    10141 gcgatcaacg cgtctttcgg catgaatgcc gtggtgcata acaccgcctg gattcaggga
    10201 cattttcacc tgaccgtcgg cagtgccacc gccctgacct tcatgggcac cgcctactgg
    10261 ctgatgccgc gactcctggg caagaacctc gagctcggac tgctggcgaa agtccagccc
    10321 tatctgtggt tccttggcat gatgctgttc tcgttctcga accacatcac ggggctcatg
    10381 gggatgccga ggcgcatcta cgacgccagc tacggcggga gcccggtggc ggagggatgg
    10441 aaggttctga ccggcgtttc ggcggcgggg ggactcctgc tcttcgtgag cgcggcgttc
    10501 ttcgtgatgg tgatggtcgg caccggcctg gccggcaagc agcgggcgtt cgccgaacca
    10561 atcctgtggg ccgaacccct ggaagacccc gggccgggga gaacgatctt cgaccgctat
    10621 gggatgtggg tgatcgccgc aatcctgatc attctcatcg cgtatgcgat accgctggca
    10681 cagcatctct cgatgccgac atttggttcg ccgggggtgc cgcagtacta ggcgtcgggg
    10741 gtcggaggtc ggggtcgggg tcggggtccc cagttacgac cttgcctgac ccctgacccc
    10801 cggaccctga cccctcagta tttggactgc atgtacctga tgccgacgtc caggatggcc
    10861 cgccagcctg tctccagcgg acccgtgaat gacggatcgc actggcggag cgtggtaatg
    10921 agcgcgtcca cgaagggccg atacatcgat gccggaatgt ccagatcctt ccggctgtgc
    10981 cgcttggcca gccgggagag caggttcggt tcgtcattca ggtgcttggg atagatcagc
    11041 aacatcccga tggcatggtg gagcagcttg tgctggcggt cgaaatcggt ctttgcgaac
    11101 cggggccggg cgtcgggaca caccttgaag aagttcgcgt agaagctcgc gtagaactcc
    11161 ggcgtccccg aagcccgttc gtagctggcg tgggctgcat cgagcgtggc ggaatcgatg
    11221 gacgcctgtg ggaccgtcat gccggctcct tggaagttcc ggggaatatg ggcccgcccc
    11281 gcgcccacgc cagcgcggcg tccacggcac gatgccactc cgggacgcgc gcgcgcgccg
    11341 cggcgccacc gatacccggt gaaaaccgcg tgaaggtccg gctggcgagg aattcctcag
    11401 ggcgcgccca cacgccgccg gcgatccccg ccaatccggc ggcaccaagc gaagtggttt
    11461 ccacgagatc cggacgcgcc accgggacgt tcaacacgtc ggcctggaac tgcatcagcc
    11521 agccgttcgc ggacgctccg ccgtccactt gcagcgcgct gagcgagaga tgcgcgtcgg
    11581 ccgtcatggc gtcgagtacg tccttggtcg aataggccat ggcctcgagc gccgcccgga
    11641 ccaggtgcgc gcgggtcgag ccccgcgtca atccgacgat ggtcccgcgc gccgcggggt
    11701 tccagtgcgg ggctcccagc ccgacgaacg ccggcacgaa cgacaccccc ccggtgtcgg
    11761 gcacgctctt cgccagcgcc tccgtctcct ccgctcgctc gatgatctgc aacccgtcgc
    11821 gcagccattg caccgcggcc cccgcgatga acacgctgcc ctcgagcgcg tacgccgggc
    11881 ggcctcgggc gtcgcatccg acggtcgtga gcagcccctg ctccgaatgg gccggctggc
    11941 cgccggtgtt catgagaaga aaggcaccgg tgccgtacgt attcttggcc tgacccgccg
    12001 tccaacaccc ctgcccgtag agcgccgcct gctgatctcc cgcaattccg aggaccggga
    12061 tctcgaaccc gaagtggtca gccaccgcca ccccgaagtc cccgctcgac gcgcgcacct
    12121 cggccagcgc gtgcctcggg acgccgaaga tcttgagcaa gccatcgtcc cagcccagtt
    12181 cgccgatgtt atagaggagt gtgcggctgg cgttggtgtg atccgtcgca aacaccctcc
    12241 caccggtgag ccgatggaga agccaggagt cgacagtacc aaataccgcg tcaccccctg
    12301 acacctgacc cctgaccccc ggcacctgat tgagcagcca ttcgatcttc gttcccgaga
    12361 agtagggatc gagcaccaga cccgtccgct ctttgatcgc cttcccctcc ggcgtccctg
    12421 cgagtgccgc gcagcggtct gccgtccggc ggtcctgcca cacgatggcc cggtgcaggg
    12481 gtttccccgt cttccggtcc cagacacaaa ccgtctcgcg ctgattcgtg atcccgatcg
    12541 ccagaggttt ggtcttcgct tgtgccaggg cctcccggca ggcgtcgaga gacgcagccc
    12601 agaggtcctc cggatcgtgc tccacccacc cggggcgggg aaagtgctgg ggaatctcac
    12661 gatagccccg ccccagcacc cggccgtcgt cagcgatgac gagcgcccgg gcgcctgtgg
    12721 tgccttcgtc gatggccagg accgatttca tggcgcgaac gtacgggtct tggctgatac
    12781 cggcaacggc ggtagcattt ccggcctatg acctatcaaa ccctgctgtt cgacgtgaag
    12841 gacggggtcg ccgtcgtcac catcaatcgc cccgacaagc tcaacgcctt gaacgaccag
    12901 gtgatggccg agctggcgga cgcggcggag cgcatcgcga cggatccggc gatcaagggc
    12961 gcgatcctga ccggagcggg gcccaaatcg ttcgtggccg gagcggacat ttcggacctc
    13021 gccaaacagg gtcccttcga cgggaaggcg cgcgccatgc gcggacaggg ggtcctgcgc
    13081 cgtctcgaga cctgcgggaa gcccgtcatc gccgcggtga acggattcgc cctgggcggg
    13141 ggctgcgaat tggcgatggc gtgccacatt cgaatcgcca gcgaaaacgc caaattcgga
    13201 cagcccgaag tgaagctcgg catcgctccc ggatatggcg ggacccagcg cctcccccgc
    13261 ctggtcggca aaggggtcgc cctgcagctg atcctgacgg ccgaaatgat cgatgccgcg
    13321 gaagcctacc ggatcggcct ggtcaacaaa gtggtcccgg ccaatgaact gatggccgaa
    13381 acggagaaaa tgctccgggg tatcctatcg atgggccccc tggcggtccg tctttcgatg
    13441 gaagcgatcg accacgggct cgagatgacg ctggacgagg gattgttgct cgaggcgaat
    13501 cacttcgggc tcctggcggc gactcaggat accaaggaag gattatccgc atttctcgag
    13561 aagcggccgg ccaaattcac cggcaagtag ccctcgccgc gttgctggcc gcgggtgcct
    13621 tggcgtgtgg cgaagatcgc ccacgccccg ggccccccga tctgtcgatc caattccagc
    13681 gcgattccct gcattcgccc gataccctcc ggggcaccat tcaggcgacc gacaacgagg
    13741 ggatcgattc ggtctggctg aacgtgggga ccaaggactc cgccgcagcg gatggcgggt
    13801 tccagttctc cttctcggcg ccatttctct tcttcatcga ctccgggctg atcaaaggcg
    13861 ccaaaatccc ggtcgaattt cgggcccgcg atatcgccgg atacagcgac gttctggaca
    13921 ccttcgtcgt catcaaatag ccctccaaaa acgtgcttaa gtcttgacct gacaataact
    13981 tcagggttac ttttcatggt actccaagtg ccacgatgac cgacccaaag cgccaaactc
    14041 cgatggctgt ttcggaggtc ctgtccggct atctcaagcg gtccggcctc gaggggcgcc
    14101 tcgcgcaggc gcggatcatc cccgactggg cgacgctggt gggggcgcaa gtcgcggcgg
    14161 tgacgatgcc ttcccgggtc acgcccgacg gaacgctgtt cgtgggcgtc cagaccagcc
    14221 catggatgaa tgagctcatg ctcatgactc cgacgatcat cgcccggctg aacgcgggac
    14281 gcggcgacgg ccgcatcaag ggtattcgct ggttcctcct aaccgaacga gaccatggcg
    14341 aaggcaaagc ctgatcccaa gcagtcgaat aatggcggtt accaggccga ctcgatccag
    14401 gtcctgaagg gcctggaggc cgtccggcgg cgccccgcga tgtacatcgg gtcggtgtcg
    14461 gcccggggac tgcaccacct ggtctacgaa gtcgtcgaca actcggtgga cgaagccctc
    14521 gccgggtact gcacccagat cgacgtctcg atccattccg acaactcgat caccgtcgcg
    14581 gacaacggac gcggcatccc ggtcgacatc cacaagaccg agaagaagcc gggcgtcgaa
    14641 gtggcgctca ccgtcctgca cgccggcggc aagttcgaca agaacaccta caaggtctcc
    14701 ggcggtctcc acggcgtcgg cgtctcggtg gtcaacgcgc tttccgaaaa gctcgaagtg
    14761 tgggtggaac gggacggcaa gaagtaccac atggcgttcg cgaagggtga caccaccaag
    14821 aaactcgagg tggtcggcaa ggcgaagacc accggcacca cggtgtggtt caagcccgac
    14881 gagaccatct tcacggaaac gcgcttcgac tatgcgacgc tggcgaaccg gctccgggag
    14941 ctcgcgttcc tgaacaaggg ccttaccatc acgctcaagg acgagcggaa gggcgggaag
    15001 accgagacct acttctacaa gggcggcctg accgagttcg tgaaatggct cctggggaac
    15061 cggaagccgc tccaccagaa ggtcatcacc ttcgaagcca acaaggacgg cgtggaagtc
    15121 gagggggccg tccagtacga cgaccagtac ggggagaaca cgttcacctt cgtgaacaac
    15181 atcaataccc acgaaggggg cacccacctc accggcttca agtcggccct cacccgcacc
    15241 atcaacgagt gggcgaagaa ggacgggctc ctcaagaagg aggagttcac gctctcgggc
    15301 gacgacgtcc gcgaggggct gaccagcgtg attcacgtca aggtcaagga cccgcagttc
    15361 gaagggcaga ccaagaccaa actcggcaac tccgaagtcg aaggcatcgt gaagagcgtg
    15421 gtcaacgaag ccctggggga cttcttcgac aagagcccca gtgtcgcccg cgccatcatc
    15481 gccaaggcga tcagcgccgc tcgcgcgcgt gaagcggccc gcaaggcccg cgagctggtg
    15541 cgccgcaaga acgcgatgga aggcgggctc ctgcccggga agctggccga ctgctcgctc
    15601 accgatccca agctctgcga gatgtacctg gtcgaaggcg actcggccgg cgggtcggcg
    15661 aaacagggac gggaccggtc cttccaggcc atcctgccgc tgcgcggcaa gatcctgaac
    15721 gtccagaaag cccggatcga caagatcctg tccaacgaag aaatccggac catgatcacg
    15781 gcgatcggga ccggcgtggg cgacgacttc aagatcgagg acgcccgcta ccacaagatc
    15841 atcatcatga cggacgccga cgtcgacggc gcgcacatcc gcaccctgct cctgacgttc
    15901 ttctacaagg aaatgaaggc cctcctggat gccgggtaca tctacatcgc gcagccgcca
    15961 ctctaccggg tcgccaaggg caaggaagag ttctacgcct acgacgaaaa agagcgggac
    16021 ggatacgcca aacgcctcgg gggcggtggc gaggaagtga agtccaacgt gatggtccag
    16081 cgctacaagg ggctcggcga gatgaacccc gatcagctct ggaagaccac gatggatccg
    16141 gccgcgcgca ccatcctcaa ggtcacgatg gaagacgcgc tcgaagcttc ccagctcttc
    16201 gaaacactga tgggcga
//
LOCUS       DASUBP010000311        10479 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1294596, whole genome shotgun sequence.
ACCESSION   DASUBP010000311 DASUBP010000000
VERSION     DASUBP010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 10479)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10479)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10479
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1294596"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /gene="tgt"
                     /locus_tag="VFU75_11815"
     CDS             <1..1104
                     /gene="tgt"
                     /locus_tag="VFU75_11815"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012270584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEU5467571.1"
                     /translation="GAARAGVLTLPHGVVETPAFMPVGTQGTVRALSPLDLENIGAGL
                     VLANTYHLHVRPGEETVAKLGGLHRFMSWNGPLLTDSGGFQVFSLEGSRTVDEDGVEF
                     QSHVDGGRRRLTPERAMEIQWTLGADIAMAFDHVAPGRVDAATSRDALERTLRWLERC
                     VKRHAALSQDAPGRQTLWPILQGGTHAELRRASLEGTLARGDWTGIAVGGLSVGEPKP
                     EMYETLSQLSPRLPPAVPRYLMGVGFPEDLVEGVRRGIDLFDCVAPTRNGRNGSAFTD
                     DGVVNIRNAAWKDDPSPLDAACDCETCTRYSRGYLRHLFAAEELLGLRLLSLHNVRYL
                     IRLAGALRRAIRQGNLDSWAAEWRLRYLNSGTA"
     gene            1108..1437
                     /gene="yajC"
                     /locus_tag="VFU75_11820"
     CDS             1108..1437
                     /gene="yajC"
                     /locus_tag="VFU75_11820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEU5467572.1"
                     /translation="MHGILAMFAPSGQTGTQPMFVLIIQFAAIGGILYFLLIRPSRQQ
                     QKKHQELLASLQKGDQVVTSGGIIGEVIFMKDNEVTIKSAESRLLVARSNITAVLNRE
                     TEGAKKS"
     gene            1434..1982
                     /gene="def"
                     /locus_tag="VFU75_11825"
     CDS             1434..1982
                     /gene="def"
                     /locus_tag="VFU75_11825"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEU5467573.1"
                     /translation="MSIRSLHLLGSPVLRERAKPVPVVDDTVRQFVDDLFDTMHAAKG
                     VGLAANQVGVPLRVAVVDVGEESPDPLVLINPVVTPRGDLIETAEEGCLSIPDIFAEV
                     DRPVDITLQALDRDGKAYTMEISGYKARAVQHEVDHLDGVLFLDHLTAVKRASLMSKW
                     RRARKGQTGYVKEVVPEPAGEL"
     gene            1982..2905
                     /gene="fmt"
                     /locus_tag="VFU75_11830"
     CDS             1982..2905
                     /gene="fmt"
                     /locus_tag="VFU75_11830"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEU5467574.1"
                     /translation="MRVVFFGTPEFAVPSLLALVGEGFDVAAVVTQPDKPQGRSRSTL
                     VSSPVKTAAVAESLPVLQPERPKDPAFIAELKRLQPDVGVVVAYGHILTPEVLAVPRL
                     GMVNVHPSLLPELRGAAPIEWAILNGLTRTGVTIMQMEAGLDSGPILHQIPHSLRDDV
                     TGGELSEHLAEVGAQALIETLAMMASDGPKPVPQHHDRATYAPKLTRETGHIVWDRDA
                     STLRRVVQALDPKPGAWTTLDGLEVKLFGAAPAAGQGAPGEVLRAGRELVVAAGSGAL
                     AIAEVQPSGKPRMSALDWIRGRGVRAGQRMV"
     gene            2902..3528
                     /locus_tag="VFU75_11835"
     CDS             2902..3528
                     /locus_tag="VFU75_11835"
                     /inference="COORDINATES: protein motif:HMM:NF014622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEU5467575.1"
                     /translation="MIAPRRSGLPRLHAVTDERIANLPDLADRSRRLAAAPVAAHARG
                     HALTGREHLALARTLGSSFTDRLFVNDRLDVALAADAVGVQLSATGLSPEEARRLRPA
                     WWIGVSVHTPEEALAAQAGGADYLLVGPVFATPTHPEASPLGVEGLARFTKFGLPVVA
                     IGGVSRANAREVVSAGAYGVAMIRALWDAPDPARAAQEIMKEMAWKSS"
     gene            3513..3713
                     /gene="thiS"
                     /locus_tag="VFU75_11840"
     CDS             3513..3713
                     /gene="thiS"
                     /locus_tag="VFU75_11840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HEU5467576.1"
                     /translation="MEIVVNGAPRTVSAGATLLELLNSWSLDPRAVVVEHNRKIVRRP
                     SLGDVRVEPGDKIELVQFVGGG"
     gene            3713..4486
                     /locus_tag="VFU75_11845"
     CDS             3713..4486
                     /locus_tag="VFU75_11845"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF017501.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1990107 - thiazole synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HEU5467577.1"
                     /translation="MDIPFVVGGQTFRSRLMIGTGKYRDNETMVRAIEATGADVVTVA
                     VRRVDLDRTADGSILKYLDPKRFFLLANTAGCYTADEAVRYARLAREAGFNDFVKLEV
                     IGDQETLLPDVAGLLEAARTLVAEGFKVLAYTNEDLITALRLEEAGCAAVMPLASPIG
                     SGLGLLNPASVRTIKRRLKVPVIVDAGVGTASDACVTMEQGVDGILMNTAVAAARDPV
                     QMGHAMRLAVEAGRAAYLAGRMPKREVAVPSSPTEGMLD"
     gene            4482..5762
                     /locus_tag="VFU75_11850"
     CDS             4482..5762
                     /locus_tag="VFU75_11850"
                     /inference="COORDINATES: protein motif:HMM:NF013362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEU5467578.1"
                     /translation="MNDATAARRAALAILRAVGRGAPFDQARDRAVATLDPRDRRLAH
                     ELAAGVLRRRAELDRTLDLSRADPRLHDVLRLGAYQLRWLDRVPAHAAVSTSVELARE
                     AAGESASGYVNQALRRLGERPAAPSEPGPTHPEWLQRRWVERYGAQGAADLADWNDAH
                     PPLTLQAARWDQARLASGLMEAGLIVEPAPWDAGLRVGKRGADETLSPREFPGFDEGA
                     FIVQDASAQLVCRYAAIPAGARCYDGCAAPGGKAVTLERRGVRVVAGDARRERLARLR
                     ENLTRAGVAIQVLCADLLSAPFPNGSWDAVVVDAPCTATGTMARHPDARWRITPDAIA
                     RAATRQRELLRAAAPLVRPGGTLVYATCSLEPEENEATVEEFLTHERTFGRAPVTDAV
                     PGGRALLTPAGDLQTLPHRDRMDGAFAARLERVR"
     gene            5762..6556
                     /locus_tag="VFU75_11855"
     CDS             5762..6556
                     /locus_tag="VFU75_11855"
                     /inference="COORDINATES: protein motif:HMM:NF015734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HEU5467579.1"
                     /translation="MRFRRHMPPLSSGTEEVSVSILPTDRRRSMLRWAGFCLGAGLVG
                     YLLAALLLFPAPLVTSRHPVPRVLGLIKSEAITDLQKAELQPKDSGSEPHPTAPAGTV
                     IWQDPPPGVDVPKDTRVSIVTSSGPSKVAVPDVAGYDAAMAQQILAAAGLTVGRTDSV
                     QAPNVAPGLVVITRPPANSVANPGTGIALVINRGAPTISVPDVVGMSLSDARDRIEGA
                     GLAVGAVTRRRVSGASPGTVIGQQPAAASLAAPGLVVDLIVARAQQ"
     gene            6553..7224
                     /gene="rpe"
                     /locus_tag="VFU75_11860"
     CDS             6553..7224
                     /gene="rpe"
                     /locus_tag="VFU75_11860"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEU5467580.1"
                     /translation="MSRGVRIAPSILSADLTRLAEQVDHVAKAGADWLHVDVMDGRFV
                     PNLTFGANMVAALKKVSSLPVDVHLMVIEPEHYIAPMADAGAANLTFHPEATIHVQRQ
                     LAAIRQRGMNAGLALNPGTPLGRAEEVLDDVDLLLIMTVNPGFGGQRYLPSSDRKIQR
                     ARAMLTSHGRDVFLEVDGGIDPDTIAAAHAAGADTFVAGQAVFGAKDPGAQVKELRRR
                     CGVVT"
     gene            7221..7754
                     /locus_tag="VFU75_11865"
     CDS             7221..7754
                     /locus_tag="VFU75_11865"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU5467581.1"
                     /translation="MTKKRLGMLGAAVAAAVLVVALVIKILPQIELVGVGSKAPDFHA
                     IDLATGTPRTLANYRGHVLLLNVWATWCEPCRVEMPAIERLHHLVTDTAFKIVSVSID
                     KEDSSGVTAFARSYGLTFPVLQNQSGDIQDLYQTTGVPESFVIDRNGVIMKKVIGAAE
                     WDAPVNVDLIHSLINAH"
     gene            7744..8676
                     /locus_tag="VFU75_11870"
     CDS             7744..8676
                     /locus_tag="VFU75_11870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467582.1"
                     /translation="MRINLTQLFTRNWALKLAALFLAILLYVAVAAQQPINQTFTLNL
                     KVRVPPGRVLRSAPPPVVVVVEGRGGELLKLRSFDKNIAVSVPDTLAGGTWHLALRPS
                     DIGIPKNADVQVTDITPRDITVMLDSVARKDVRIVARVTVSADSGGVLQGGLAITPNQ
                     AHLVGPDQLLATIESVTTVPTELSGLSGTFARAVPLDTSNLGVVRVAPREVQVSGEVA
                     VPAHRVFGGIAVETGAGVPTGFVFTPDRVSVEVAGAPQRLANLTRDSVKVIARFSGAA
                     TAGAAAHLTVLTPPGVAGHAVPDSAQLKVAPRHK"
     gene            8673..9734
                     /gene="tsaD"
                     /locus_tag="VFU75_11875"
     CDS             8673..9734
                     /gene="tsaD"
                     /locus_tag="VFU75_11875"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEU5467583.1"
                     /translation="MSDVILGIETSCDETSAAVLVRNGSAPPELASLVILSQDIHQVF
                     GGVVPELASRAHLAALPAVVERALDQAGVAFGAVDAVAVTSGPGLVGALLVGVVYAKS
                     LAYAGNRALIGVNHLEGHLFAPAVEDAELAPPFVALLVSGGHTMLLDVPEWGRYRLMG
                     ATRDDAAGEAFDKVAILMGLGYPGGPVIERLAKEGDPTRFTFPRPMKDDGFEFSFSGL
                     KTAVLHAVKASDDLERDRPHLARGFQDAVLDVLVTKLDRAVAATGYRTAVLGGGVACS
                     KALAARAAERLKGKARVSVASPRLNTDNAGMIARAGWWHLERGERSDFTLDARADLPI
                     PGLELFQSSIPNPQFPGSR"
     gene            9731..>10479
                     /locus_tag="VFU75_11880"
     CDS             9731..>10479
                     /locus_tag="VFU75_11880"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HEU5467584.1"
                     /translation="MTVYPFVLHIGFLTVTGYGLMMMAAFLAAGYAIQLELRRRGLDE
                     EYAASIVWWAVIGGLVGAKLWYVALTHDMGVLFKRGGFVWYGGLIGGSGGVLLAGWLR
                     KVPWRFTADLVAGPLALGYALGRVGCFLVNDDYGIPTSLPWGMKFPQGLPPTTIGELK
                     LLHVSFPVGVRDTDVVAVHPTEIYETAIMLFVFWWLWKRRNHSHGMGWLFGCYLILSG
                     AERFLIEFLRAKDDRLLGPFTLAQATALIIVA"
BASE COUNT         1589 a         3623 c         3567 g         1700 t
ORIGIN      
        1 ggtgccgccc gcgccggggt gctgaccctc cctcacgggg tcgtcgagac gccggcattc
       61 atgcccgtgg gcacccaggg aacggtccgc gcgctgtcac ccctcgacct cgagaacatc
      121 ggcgcggggc tcgtgctcgc gaacacttat cacctccacg tccgccccgg cgaggaaacc
      181 gtcgcgaagc tcggcgggct ccaccgcttc atgtcgtgga acggtccgct cctcaccgac
      241 tcgggcgggt tccaggtctt ctcactggaa ggatcgcgca ccgtcgacga ggacggggtc
      301 gaatttcaga gtcacgtgga cggcggccgg cgccggctca cccccgagcg cgccatggaa
      361 attcaatgga cgctgggcgc ggacatcgcg atggcgttcg atcacgtggc accgggcaga
      421 gtcgatgccg ccaccagccg tgacgcgttg gagcggacac tccgctggct cgagcggtgc
      481 gtgaagcgac acgcggcgct gtcgcaggac gcacccggac ggcagaccct gtggcctatc
      541 ctgcagggcg gcacgcacgc cgagctccgg cgggcgtcac tcgagggcac cctggcgcgg
      601 ggcgactgga ccggcatcgc ggtgggcggg ctgtcggtcg gtgaaccgaa acccgagatg
      661 tacgagacgc tgtcacaact atctccccgg ctcccccctg ccgttccccg ctatcttatg
      721 ggcgtcggat tcccggaaga tctggtggag ggcgttcggc gcggcatcga cctgttcgac
      781 tgcgtggcac ccacgcggaa cgggcggaac ggatcggcat tcaccgatga cggtgtggtc
      841 aatatccgga acgcggcctg gaaggacgat ccgtcccccc tggacgccgc ctgcgactgc
      901 gagacctgca cccgctactc ccgcgggtac ctgcgtcatc tcttcgcggc ggaagagctg
      961 ctcggactcc ggctgttgtc gctgcacaac gtgcggtacc tgattcggct ggcgggcgcg
     1021 ttgcgccggg ccatccggca ggggaacctg gattcgtggg ccgctgaatg gcggctccgc
     1081 tacctcaact cgggaacggc ctaattcatg cacggcatcc tcgcgatgtt cgcaccttcc
     1141 ggccagaccg gcacgcagcc gatgttcgtg ctgatcattc agttcgcagc catcggcggc
     1201 atcctgtact tcctgttgat ccgtccgtcg cgccagcagc agaagaagca ccaggagctg
     1261 ctcgcgtccc tgcagaaagg tgaccaggtc gtcacgtcgg ggggtatcat cggcgaggtg
     1321 atcttcatga aggacaatga agtgacgatc aagagcgccg agtcgcgtct gctggtggcg
     1381 cgcagcaaca tcaccgcggt cctgaaccgc gagaccgagg gggcgaagaa gtcgtgagca
     1441 tccggtccct ccacctgctg ggatcaccgg tcctgcgcga acgagccaag ccggtgccgg
     1501 tggtggatga caccgtccgt cagttcgtgg acgacctgtt cgacaccatg cacgccgcga
     1561 agggcgtcgg gctcgccgcc aaccaggtag gcgtcccact gcgcgtggcc gtggtggacg
     1621 tgggagagga gtcccccgac ccgctggtcc tcatcaatcc cgtggtgacc ccgcgtggcg
     1681 acctgatcga gacggccgaa gaagggtgcc tctccatccc cgacatcttc gccgaagtcg
     1741 atcgcccggt ggacatcacg ctccaggcgc tcgatcgcga cggcaaggcg tataccatgg
     1801 agatctccgg ctacaaggcc cgggccgttc agcacgaggt cgaccacctc gacggcgtac
     1861 tgttcctcga tcatctcacc gcggtcaagc gcgcgtcgct gatgtccaaa tggcgccgcg
     1921 cccggaaggg ccagaccggc tacgtgaagg aagtcgttcc ggagccggcg ggcgagctct
     1981 agtgcgggtc gtgttcttcg gcacgccgga gttcgcggtg ccatcgctcc tcgccctggt
     2041 aggtgaagga ttcgacgtcg cagccgtggt cacacaaccc gacaaaccgc agggacgttc
     2101 ccgctcgacc ctggtatcct ccccggtcaa gaccgcagcc gtcgccgaga gtctgcccgt
     2161 cctgcaacct gagcggccga aagatcccgc gttcatcgcg gagctgaaga ggctccagcc
     2221 cgacgtgggc gtggtcgtcg cctacgggca catcctgacc cccgaggtgc tggccgtccc
     2281 ccgcctcggc atggtcaacg tgcatccctc tctcctgccc gagctccgcg gcgccgcccc
     2341 catcgagtgg gccatcctga acggcctcac gagaaccggc gtgaccatca tgcagatgga
     2401 ggcgggactc gactccggac ccattctcca ccagatcccc cactccctcc gggacgatgt
     2461 cacggggggt gagctctcgg aacatctcgc ggaagtcggc gcgcaggcgc tcatcgagac
     2521 gctcgcgatg atggccagtg acgggccgaa accggttccc cagcatcatg atcgcgcgac
     2581 ctacgcgccc aagctcacgc gcgagaccgg ccacatcgta tgggatcgcg acgcgtccac
     2641 cctgcgtcgt gtggtccagg ccctcgatcc gaaacccggt gcctggacca ccctcgacgg
     2701 cctcgaagtg aaactcttcg gcgcggcgcc ggccgccggc cagggcgccc ccggcgaggt
     2761 acttcgtgcc gggcgcgagc tcgtcgtcgc ggcaggctcg ggagcgcttg cgatcgccga
     2821 ggtgcaaccg tccggcaagc cgcgcatgag cgcgctcgac tggattcgag gccggggcgt
     2881 gcgggccggc cagcgaatgg tgtgatcgcg cctcgccggt ccgggctccc gcgactgcac
     2941 gcggttaccg acgaacggat cgccaacctc cccgatctcg ccgaccggtc caggcgcctc
     3001 gccgccgccc ctgtcgccgc ccatgcccgc ggccacgcac tcaccggccg cgagcatctc
     3061 gcactcgcac gcaccctcgg ctcctccttc accgaccggc tcttcgtgaa cgaccgtctc
     3121 gacgtggcgt tggccgccga tgccgtcggg gttcagctgt ccgccaccgg cctgagcccc
     3181 gaggaagcgc gccgcttgcg accggcctgg tggatcggcg tgtccgtcca caccccggaa
     3241 gaggccctgg ccgcgcaagc cggaggggcc gactatcttt tggtaggacc ggtctttgcg
     3301 acccccacgc atccggaggc ctcaccgctg ggcgtggaag gactggcccg gttcaccaag
     3361 ttcggactcc ccgtggtcgc gatcggtggg gtaagccggg ccaacgcgcg ggaagtcgtg
     3421 tcggcgggcg cgtatggggt agcaatgatc cgggccctgt gggacgcgcc ggacccggcc
     3481 cgcgcggcgc aggagataat gaaggagatg gcgtggaaat cgtcgtgaat ggagcgcccc
     3541 gcacggtctc ggcgggtgcg acgctcctgg agctcttgaa cagctggtcg ctggatccgc
     3601 gggctgtggt cgtggaacac aaccgcaaga tcgtacggcg cccctcactg ggagacgtgc
     3661 gcgtggagcc cggcgacaag atcgagctgg tgcagttcgt aggaggtggc tgatggacat
     3721 tcccttcgtc gtgggcggtc agaccttccg ctcccgcctg atgatcggta ccggcaagta
     3781 ccgggacaac gagaccatgg tccgcgccat cgaagcgacg ggggcggacg tcgtcaccgt
     3841 agcggtccgg cgcgtggatc tcgatcgcac ggccgacggc agcatcctga agtatctgga
     3901 tcccaagcgg ttcttcctgc tcgccaacac cgcgggctgc tacaccgccg acgaagcggt
     3961 gcggtacgcg cgcctcgccc gcgaggcagg cttcaatgac ttcgtgaagc tcgaagtgat
     4021 cggcgaccag gaaaccctgc tcccggacgt cgccggtctg ctcgaggccg cccgcaccct
     4081 ggtggccgaa gggttcaaag tcctggcgta cacgaatgag gacctgatta cggcgttgcg
     4141 gctcgaagaa gcgggttgcg cggccgtcat gcccctcgcc tcccccatcg gctcggggct
     4201 cgggctcctc aatccggcga gcgtgcggac catcaagcgc cgcctgaagg tgccggtgat
     4261 cgtggacgcg ggcgtgggga ccgcgtccga tgcgtgcgtc accatggaac agggcgtcga
     4321 cggcatcctg atgaacacgg ccgtcgcggc ggcgagggat ccggtgcaga tgggtcacgc
     4381 catgcgtctc gccgtggaag cggggcgggc cgcgtatctc gcgggccgga tgccgaagcg
     4441 cgaagtggcc gtgccgtcct cccccaccga ggggatgctg gattaacgac gcgaccgcgg
     4501 cgcgccgggc ggcgctcgcg attctccgcg ccgtcggtcg tggtgcgccg ttcgaccagg
     4561 cccgcgatcg ggcggtcgca acgctcgatc cccgggaccg ccgactcgcc cacgagctcg
     4621 ccgccggcgt gctgcggcgg cgcgccgagc tggaccggac cctcgacctc tcccgcgcgg
     4681 accccagact ccatgacgtc ctgcgcctcg gggcctatca gctccgctgg ctggaccggg
     4741 tacccgcgca cgccgccgtt tccaccagcg tggagctggc gcgcgaagcc gcgggcgagt
     4801 cagcgagcgg ttacgtcaat caggccctgc gccgcctcgg cgaacggccc gcggctcctt
     4861 ccgagccggg ccccacgcac cccgagtggc tgcaacgccg gtgggtcgag cgctacgggg
     4921 ctcagggagc ggcggatctg gccgactgga acgacgcgca cccgccgctc acgctgcaag
     4981 ccgcgcgctg ggaccaagcc aggctcgcct caggtctgat ggaagcgggc ctgatcgtgg
     5041 aacccgcgcc gtgggatgcg ggtctgcgag tcggcaaacg cggggcggac gaaacccttt
     5101 ccccgcgcga gttcccggga ttcgacgagg gcgcgttcat cgttcaagac gcctcggccc
     5161 agctggtctg ccggtatgcg gcgattcccg ctggtgcacg ctgctacgat gggtgcgccg
     5221 cgccgggggg caaggcggtc acgctggagc ggcgcggcgt acgcgtcgtg gccggcgacg
     5281 cccggcggga gcggttggcg cgactgcgcg agaaccttac gcgggccgga gtggcgattc
     5341 aggtgctttg cgccgatctt ctttccgcgc ccttcccgaa tggcagctgg gacgcagtgg
     5401 tggtggatgc gccctgcacc gccaccggca ccatggcccg acacccggac gcgcgttggc
     5461 gcattacacc cgacgccatc gcacgcgccg ccacccggca acgggagttg ctccgtgcgg
     5521 cggcgccgct ggtccggccc ggcggtacgc tggtgtacgc gacgtgttcg ctggaacccg
     5581 aggagaatga ggccaccgtg gaggaattcc tgacgcacga gcggaccttc ggccgtgcgc
     5641 cggtgaccga tgcggtcccc ggcgggcgtg ccctgctcac gcccgcggga gacctccaga
     5701 ctcttcccca ccgcgaccgg atggacggcg cattcgcggc ccggctggag cgcgtgcgct
     5761 gatgcgtttc cgccggcaca tgcctccgct ctcgtcgggg acggaagagg tgagcgtctc
     5821 gatactgccc acggatcgac gccgcagcat gctgcgatgg gcggggttct gcctgggggc
     5881 gggactcgtc gggtatctgc tcgccgcgct cctgctcttt ccggcgcccc tcgtgacctc
     5941 gcggcatccc gtgccccggg tgctgggcct gatcaagtcg gaggcgatta ccgacctgca
     6001 aaaggccgaa ctgcaaccca aggactcggg ctccgagccc catcccacgg cccccgccgg
     6061 cacggtgatc tggcaagacc cgccgcctgg cgtagacgtg ccgaaagaca cccgggtgag
     6121 catcgtcacc agcagcggcc catccaaagt ggcagtcccg gatgtcgcgg gttacgatgc
     6181 cgccatggct caacaaatcc tcgcggccgc cggtctgacc gtgggccgca ccgactcggt
     6241 gcaggccccg aacgtggctc ccggcctggt ggtgatcacc cgcccgccgg ccaacagcgt
     6301 cgcgaatccc ggtaccggaa tcgcactcgt catcaaccgc ggggccccaa ccatctccgt
     6361 gcccgatgtg gtgggcatgt ccctctccga cgcgcgcgac cggatcgagg gtgcgggcct
     6421 agccgtcggc gccgtgacgc gccggcgcgt atccggcgcg agtcccggca cggtgatcgg
     6481 gcagcaaccc gcggcggcgt ccctcgcggc accgggactc gtggtggacc tcatcgtggc
     6541 acgggctcag caatgagccg cggcgtacgc atcgccccga gcatcctctc cgccgacctg
     6601 accaggctcg ccgagcaggt ggatcacgtg gcgaaggcgg gggccgactg gctgcacgtg
     6661 gacgtcatgg acggccgctt cgtacccaac ctcaccttcg gtgcgaacat ggtcgccgca
     6721 ctcaagaagg tgtcgagcct gccggtggac gtgcatttga tggtgatcga gcccgagcac
     6781 tatatcgcgc cgatggcgga cgccggggcc gcgaacctca cgtttcatcc ggaggccacc
     6841 attcacgtcc agcgccagct cgccgccatc agacagcgtg gcatgaacgc cgggctcgcg
     6901 ctgaaccccg gcaccccgct gggccgcgcc gaagaagtgc tggatgacgt cgatctcctg
     6961 ctgatcatga cggtgaatcc gggcttcggc ggacaacgct accttccgtc gtccgaccgg
     7021 aagattcagc gggccagggc gatgctgacg tcccatggac gggatgtgtt tctcgaagtg
     7081 gacgggggca tcgatcccga cacgatagcc gccgcgcatg cggccggagc cgatacgttc
     7141 gtcgcgggcc aggcggtgtt tggggccaaa gacccgggag cccaggtgaa ggagctccga
     7201 cgacgctgcg gagtggtgac atgacgaaaa agcgcttggg gatgttgggt gcggccgtgg
     7261 ccgccgccgt tctcgtggtg gccctggtga tcaagattct gccccagatc gagctggtgg
     7321 gggtcggctc caaggcaccg gattttcacg ccatcgacct ggccaccgga actccccgga
     7381 cgctggccaa ctaccgcggt cacgtgctgc tcctgaatgt ctgggcgacg tggtgtgagc
     7441 cctgccgcgt cgagatgccg gcgattgagc ggctccatca cctggtgacc gatacggcgt
     7501 tcaagatcgt ttccgtcagc atcgacaagg aagactcgag cggcgtcacg gccttcgcgc
     7561 ggtcgtacgg tctcaccttc ccggtcttgc agaatcaaag cggggacatt caggacctct
     7621 atcaaaccac cggagttccg gagagctttg tgatcgaccg gaacggggtg atcatgaaga
     7681 aggtcatcgg tgccgccgag tgggacgcgc cggtcaatgt ggatctgatc cactccctga
     7741 tcaatgcgca ttaacctgac gcagctcttt acccggaact gggccctcaa gctggccgcg
     7801 ctctttctcg cgatcctcct gtatgtcgcg gtcgccgctc agcagcccat caatcagacg
     7861 ttcaccctga atctcaaggt ccgggtaccc cccggccgcg tgctgcgcag cgccccaccc
     7921 cccgtggtcg tggtcgtcga gggccgcggc ggggagctgc tcaagctgcg gagcttcgac
     7981 aagaacatcg ccgtgtccgt tcccgacacg ctggcgggtg gcacctggca cctcgcactc
     8041 cgcccttcgg acatcggtat tccgaagaac gccgacgtgc aggtcaccga catcacgccg
     8101 cgcgacatca ccgtgatgct cgactccgtg gcccggaagg acgtgcggat cgtcgcgcgc
     8161 gtcaccgtgt ccgcggactc gggtggcgtg ctccagggcg gcctcgcgat cactcccaac
     8221 caggcgcacc tggtggggcc cgatcagctg ctcgcgacca tcgagtcggt caccaccgtt
     8281 cccaccgagc tctcgggcct ttcggggacg ttcgcgcgcg ccgttccgct cgacacgtcc
     8341 aacctgggcg tcgtccgcgt ggcgccgcgg gaggtgcagg tttcgggtga ggtggcggtg
     8401 ccggcccacc gcgtcttcgg cggcattgcc gtcgaaaccg gcgcgggggt tccgaccgga
     8461 tttgtcttca cccccgatcg cgtctccgtg gaagtggccg gcgcaccgca gcggctcgcc
     8521 aatctcacac gggactcggt gaaggtcatc gcgcggttca gcggcgcggc cacggcgggt
     8581 gcggccgctc accttaccgt gctgacgccg cccggagtcg cggggcacgc cgttcctgat
     8641 tccgcgcagc tgaaggtcgc gcccagacac aaatgagcga cgtcatcctc ggcatcgaga
     8701 cctcgtgcga cgagacctcc gccgccgtgc tggttcggaa cggctcggcg cctccggagc
     8761 tcgcgagcct ggtgattctc tcacaggaca tccaccaggt gtttggcggc gtcgtgcctg
     8821 agctcgccag ccgcgcgcat ctcgcggcgt tgccggcggt ggtcgagcgg gccctcgatc
     8881 aggcgggcgt ggcattcgga gcggtcgacg ccgtggcggt cacgtcgggt ccgggcctgg
     8941 tcggtgcgct gctcgtcggc gtcgtgtacg ccaagtccct cgcctatgcg gggaatcgcg
     9001 cgttgatcgg cgtgaaccat ctggaggggc acctgttcgc acccgccgtc gaagatgcgg
     9061 agctcgcgcc ccccttcgtc gctctgctcg tgtcgggcgg ccacaccatg ctcctcgacg
     9121 tgcccgagtg gggacgctac cggctcatgg gagcgacgcg ggacgacgcg gccggcgagg
     9181 cgttcgacaa ggtcgccatc ctgatgggtc tggggtatcc cggtggcccc gtgatcgagc
     9241 gcttggccaa ggagggtgac cccacgcgat tcaccttccc gcggcccatg aaggacgacg
     9301 ggttcgagtt ctccttctcc ggcctcaaga ccgcggtcct gcacgccgtg aaagcgagtg
     9361 acgacctgga acgggaccgt ccgcacctgg cgcgcgggtt tcaggacgcg gtactggacg
     9421 tgctcgtcac caagctcgac cgtgccgtcg ccgcaaccgg atatcgaacc gccgtactcg
     9481 gcggcggcgt ggcctgcagc aaggcgctgg cggcccgggc cgcggagcgg ctcaagggca
     9541 aggcgcgcgt gtccgtcgcg agcccccgtc tgaacaccga caacgccggc atgatcgccc
     9601 gcgcgggctg gtggcacttg gagcgcggcg agcgcagcga cttcacgctc gacgcccgcg
     9661 ccgatctgcc catccctggg ctggagctct tccaatcctc catcccgaat ccccaattcc
     9721 cgggatctcg atgaccgtct accccttcgt cctgcatatc ggatttctca cggtcaccgg
     9781 ctacggcctg atgatgatgg ccgccttcct cgccgccgga tacgcgattc agctcgagct
     9841 caggcggcgc ggtctcgacg aggaatacgc cgccagtatc gtctggtggg cggtgatcgg
     9901 cggactggtg ggcgccaagc tctggtacgt ggcgctgacg cacgacatgg gcgtgctctt
     9961 caagcgtggc gggttcgtgt ggtacggggg cctgatcggc gggtcgggcg gcgtgctgct
    10021 cgcgggctgg ctccgcaagg tcccgtggcg cttcaccgcg gatctcgtgg ccggtcccct
    10081 cgcgctcggt tatgcgctcg gccgcgtcgg gtgcttcctg gtgaatgacg actacggcat
    10141 cccgacatca ctcccctggg gcatgaagtt cccgcagggc cttcctccga ccaccatcgg
    10201 ggagctcaag ctgctgcacg tcagcttccc cgtgggcgtc agggatacgg acgtggtcgc
    10261 ggtgcatccg accgagatct acgagaccgc gatcatgctg ttcgtgttct ggtggctctg
    10321 gaagcggcgg aaccattccc acggcatggg ctggcttttc gggtgttacc tgatcctgtc
    10381 cggggcggag cggttcctga tcgaattcct gcgcgcgaag gatgaccgcc tgctgggacc
    10441 tttcacgctg gcccaggcga cggcgctgat catcgtggc
//
LOCUS       DASUBP010000312         3445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_871914, whole genome shotgun sequence.
ACCESSION   DASUBP010000312 DASUBP010000000
VERSION     DASUBP010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 3445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3445
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871914"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VFU75_11885"
     CDS             <1..604
                     /locus_tag="VFU75_11885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467585.1"
                     /translation="QLVRVTVSALSQSYPCRYGAPVIAAVDPAIFSRQYFTHCLECSF
                     CHDQCCSYGVDYDWRVRENLQKHADAFETFSGVSRGRWFTGDTTPDESMPGGGSDRTA
                     VENGACVFLNRGARGCQIHAYALAAGVDYHDLKSIVDCLFPLTWEGHELGLSEEVLDS
                     SLICLDSGPTVYRGVREELRFYFGDGLVEELDRLEAALRA"
     gene            complement(594..863)
                     /locus_tag="VFU75_11890"
     CDS             complement(594..863)
                     /locus_tag="VFU75_11890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467586.1"
                     /translation="MAETAKRCLYCGAPQYKAAPGTPERAAEETAALEEEKKLARQAA
                     LYRAGVGMAKGEATPDQGAGLLKALLMLFVNPISSFKILRKIFRP"
     gene            complement(917..1570)
                     /locus_tag="VFU75_11895"
     CDS             complement(917..1570)
                     /locus_tag="VFU75_11895"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEU5467587.1"
                     /translation="MRRMWMAAALLAALAACSKQDKGTATQTAPADAAALLNPASPAM
                     TAQAPGTYRAKFETSAGDFTIEVTRAWAPLGADRLYNLIKGGFYDGDRFFRVVPGFVV
                     QFGLNGNPAVSAAWRHANIQDDPVTQHNNTGTIVFATAGPNTRTTQLFINYADNLRLD
                     GMGFAPLGKVVDGMDVVTKIFPDYGQTPDQSLIESQGNGYLAQAFPKLDYIKRATIQ"
     gene            1673..2479
                     /locus_tag="VFU75_11900"
     CDS             1673..2479
                     /locus_tag="VFU75_11900"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HEU5467588.1"
                     /translation="MSLSKKLRVPPGTRVSLDQWDPADTHGWKKDERMEKELIESIRK
                     LDALQYVMYAERKHALLVVLQGMDGAGKDGTIRHVMTGLNPQGCRVTPFKVPTPEEAS
                     HDFLWRVHRAIPQLGDIGIFNRSHYEDVLVARVHNLVPKAVWSRRYDDINAFESLLAE
                     QGVVIVKCFLHISKEEQKERFEDRINDKDKQWKLSAADFAERKYWGAYQKAFEDALSK
                     CSTERAPWYVIPADKKWFRNLAVCRILVETLEGLRMKFPKPSVDISKLDV"
     gene            complement(2476..2697)
                     /locus_tag="VFU75_11905"
     CDS             complement(2476..2697)
                     /locus_tag="VFU75_11905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467589.1"
                     /translation="MNNSRSQLAKSGLLADGGAPRCPNCRTRLSFTTDRQGHVLEACP
                     CGYRALVVPRRSVEPGADVPVPPVPSPKP"
     gene            complement(2694..2912)
                     /gene="infA"
                     /locus_tag="VFU75_11910"
     CDS             complement(2694..2912)
                     /gene="infA"
                     /locus_tag="VFU75_11910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012039277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEU5467590.1"
                     /translation="MAKEEPIQLDGVVADVLPNSTFRVQLPNGHDVLATLAGKMRRFR
                     IRVLAGDRVTLDVSPYDLTRGRITFRHK"
     gene            complement(3047..>3445)
                     /locus_tag="VFU75_11915"
     CDS             complement(3047..>3445)
                     /locus_tag="VFU75_11915"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13-type metalloendopeptidase"
                     /protein_id="HEU5467591.1"
                     /translation="KEADKIVAQFSGYLQVDTIHGNGKLTEGENIADLGGVLTGYDAL
                     ERALQRDGRPGLIEGFTPEQRYFIAYAQSWRTHTRDATLRTWVKTNPHAPDYWRANGP
                     LANDPEFAKAFGCKPGDPMVRAPDLVPHIW"
BASE COUNT          596 a         1134 c         1082 g          633 t
ORIGIN      
        1 gcaacttgtt cgtgtgaccg tcagcgccct ctcgcaatcc tacccctgcc gctacggcgc
       61 gccggtcatc gccgccgtcg atcccgccat cttctcgagg cagtacttca cccattgcct
      121 cgagtgctcg ttctgccacg accaatgctg ctcatacggc gtggactacg actggcgggt
      181 ccgcgaaaac ttgcagaagc acgccgatgc gttcgagacc ttctccggcg tctcccgcgg
      241 ccgctggttc acgggtgata ccacccccga tgagtccatg cccgggggcg gctccgaccg
      301 cactgcggtc gagaatggcg cctgtgtctt cctcaaccgg ggcgcacgcg gatgccagat
      361 ccacgcctac gcgctcgccg ccggcgtcga ttatcacgac ctcaagtcca tcgtggactg
      421 cctcttcccg ctgacgtggg aaggccacga gctggggttg tcggaagagg tgctcgactc
      481 gagcctgatc tgcctcgact cgggccccac ggtgtaccgc ggggttcggg aggagctgcg
      541 tttctacttc ggagacggcc tggtggaaga gctcgatcgg ctcgaggccg ccctcagggc
      601 ctgaagatct tccgcaagat cttgaacgaa gagatcggat tcacgaagag catgagcagg
      661 gccttgagca gccccgcgcc ctggtccggc gttgcctctc cctttgccat gccgacgccg
      721 gcgcgataga gcgccgcctg ccgcgccagc ttcttttctt cctccagcgc cgccgtctct
      781 tccgccgccc gctccggagt gccgggcgcc gctttgtact gcggtgcccc gcagtacagg
      841 cagcgtttcg cggtctccgc caccggccga ccgcacttcg aacacggtgc ggtcgggact
      901 ctggtatccg ccatgtttac tgaatcgtcg cccgtttgat gtagtcgagc ttcgggaacg
      961 cctgcgccaa atagccgttc ccctggcttt cgatgagact ctgatcgggt gtctgcccat
     1021 aatcggggaa gatcttcgtc accacgtcca tcccgtccac caccttgccg agcggcgcga
     1081 atcccatccc gtcgagccgc aggttgtccg cgtaattgat gaagagctgc gtggtacgcg
     1141 tgttcgggcc cgccgtcgcg aagacgatcg taccggtgtt gttgtgctgc gtcaccggat
     1201 catcctggat gttcgcgtgc cgccaggccg cactcacggc gggattgccg ttgagtccga
     1261 attgcacgac gaagcccggg accacccgga agaaccggtc gccgtcgtag aacccgccct
     1321 tgatcaggtt gtagaggcgg tcggcgccga ggggcgccca ggcgcgggtg acttcaatgg
     1381 tgaagtcgcc ggccgatgtt tcgaacttgg cgcgataggt gccaggcgcc tgcgcggtca
     1441 tcgccggtga cgccggattg agcagggccg ccgcatcagc gggagcggtt tgagtcgcgg
     1501 tccccttgtc ctgcttgctg cacgcggcga gagcggccag cagcgcggcg gccatccaca
     1561 tccttcgcat cgggattccc ttcgtgaggt ttggtcttgc ctgtacgtct gagcgtccta
     1621 gccgtctagc cattttggaa tatgttgagc gccccatacc acgggcaagc cgatgtccct
     1681 ctccaagaag ctgcgggtgc cacccggaac ccgcgtctca ctcgatcagt gggaccccgc
     1741 cgacacccac ggctggaaga aggacgagcg aatggagaag gagctgatcg agagcatccg
     1801 caagctcgac gcgctccaat acgtgatgta cgccgagcgc aaacatgccc tcctggtcgt
     1861 gctccaggga atggacggtg ccgggaaaga cggcaccatc cggcatgtga tgacgggtct
     1921 caacccccag ggatgccggg tgacgccctt caaggtcccg acccccgaag aagcctccca
     1981 cgactttctc tggcgcgtcc atcgtgccat tccgcaactc ggcgatatcg gcatcttcaa
     2041 tcgctcgcac tacgaagatg tgctggtggc ccgggtccac aacctcgtcc cgaaggcggt
     2101 ctggtcccgc cgctacgacg atatcaacgc cttcgaatca ctgctggccg aacagggcgt
     2161 cgtgatcgtg aaatgcttcc ttcatatctc gaaggaagag cagaaggagc gcttcgagga
     2221 ccggatcaac gacaaagaca agcagtggaa gctctcggct gcggactttg ccgagcgcaa
     2281 gtactgggga gcctaccaga aagccttcga agacgcgctg tcgaaatgca gcaccgagcg
     2341 ggcgccctgg tacgtcattc ccgccgacaa gaagtggttc cgcaacctgg ccgtgtgccg
     2401 gatcctggtg gaaacactcg agggcttgag gatgaagttc cccaagccct cggtcgacat
     2461 ttccaaacta gacgtctagg gcttggggct cggcaccggc ggcaccggca cgtcggcgcc
     2521 cggctcgacg cttcgccggg gcaccaccag cgcgcgatac ccgcacggac acgcctcgag
     2581 cacatgcccc tgacggtcgg tggtgaagct cagccgggtc cggcagttgg gacagcgggg
     2641 tgccccgccg tcggccagga gcccgctttt cgcgagctgg gaccgggaat tgttcacttg
     2701 tggcggaacg tgatgcggcc gcgggtcaga tcataaggcg acacatcgag ggtgacgcgg
     2761 tcgcccgcca gaacgcgaat ccggaaccgc cgcatcttgc cggccagcgt cgccaggacg
     2821 tcgtgcccgt tggggagctg aacccgaaag gtgctgttcg ggagcacgtc ggccaccaca
     2881 ccgtccaact ggatcggctc ttctttcgcc atgacgactc ccaaaaaaag aaggtgggac
     2941 ttggcgcatt ggccaagtcc caccttgacg gtttgctggg gcgatccgca ccgggcggac
     3001 cgcgttccag aacactaacg gggcgtaccg ccgaagtcaa aaacgactac cagatgtgcg
     3061 gcaccaggtc aggagcgcgg accatcggat cacccggctt gcacccgaag gcctttgcga
     3121 actcgggatc gttggccagc gggccgttcg cgcgccagta atcaggcgcg tgcgggttgg
     3181 tcttcaccca cgtgcggagt gtggcgtcgc gggtgtgggt tcgccagctc tgcgcgtagg
     3241 cgatgaagta gcgctgctcc ggcgtgaagc cctcgatgag ccccggccga ccatcccgct
     3301 gcagcgcgcg ttccagcgcg tcgtaacccg tcaggacccc gccgagatcg gcgatgttct
     3361 cgccttccgt cagcttgccg ttgccgtgaa tcgtgtccac ctggagatat ccggagaact
     3421 gcgccacgat cttgtcggct tcctt
//
LOCUS       DASUBP010000313        13260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_381464, whole genome shotgun sequence.
ACCESSION   DASUBP010000313 DASUBP010000000
VERSION     DASUBP010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 13260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13260
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381464"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="VFU75_11920"
     CDS             complement(<1..1180)
                     /locus_tag="VFU75_11920"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEU5467592.1"
                     /translation="MRDGIALIYLLVFIAPQAVLYVYLRDRLPDRSRPRQAHIVRWSL
                     ILLFAFFNLPWIFVARRVLFGSVWSKGWIPYIGPWVAWQMLGWIFLGLVAVYVLFKGV
                     VLCVRGLRRSERLPTPGSQVTRRQFLVRGTYAYAGAGLALSAYGLWEGARLPEITRRT
                     LYFDHLPPGLDGLKILHLSDVHAGIHMRLEKMQRLVAQANALAPDLILQTGDMIDISP
                     SFIPDYVRAFRELSAPLGVITGLGNHDHYTGAAEVSKGVQDAGQTLVRSGHHLIERNG
                     ATLAILGVDDPLDWRFDDPQAAEVSRVDREAPPDTFKILMAHRPGAWDAAKLHNIPLT
                     LSGHIHGGQFYLPVVGWSAGRLITKYVMGHFQDGASQLYVSRGIGVVGVPIRVFVPPE
                     M"
     gene            1167..2489
                     /locus_tag="VFU75_11925"
     CDS             1167..2489
                     /locus_tag="VFU75_11925"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HEU5467593.1"
                     /translation="MPSRIPLLLLAAAPAVLGVRTATGQQLSATDRARMVATLWSDAR
                     YNYAYWDRVEADWDSALDANLAFAAQRQSDVTFWRRLEVLAALLNDGQAAVVPPVAVR
                     SRTARPPIRIRAVEGRPFVADYAENDELRIARPGRGFEIVSVQGVLAATWIRDSILPG
                     IPGSTAATRWERAVASMLVGERGTPVQLALKGPGGVDRGASLTRSVATSARSPFEPPP
                     LAVDTVSGGISVVHLNSCAGAGVVSQFDRTFTTWTGVRALILDLRENDGTGGGRESCY
                     RILARLMSHPFVTSRWRTPEYRPAYRGADMPDSAGAWFSAPPDTIVPRPDLPVFNGPV
                     ALLSSARTAGAGAGADLLVAFRNAQRGPIVGEPSAGGTGQVLTLPLSRGWFFRVTVTR
                     DAFPDGSEFAATGIAPEMAVTETVADFQSGKDAVLERAVAYLRERTSR"
     gene            complement(2605..3384)
                     /locus_tag="VFU75_11930"
     CDS             complement(2605..3384)
                     /locus_tag="VFU75_11930"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU5467594.1"
                     /translation="MASDRLEKLSLGAVALLAVALPAWRLATPRPQPVDIHSEYVKYL
                     SGKDSITAYLAYPERSDAAPGVIVIHEIFGMSDFVKDRVTQLAKDGFVAIAPDLLSRR
                     GGTPASQDQAIQMIRGLNPDTLTQDLDATYQFLAHVKAVRADRIGVIGFCWGGGQSFR
                     YATNNPQLKGFVVCYGPGPDSASLARLKAPGLGVYAEKDARISLSVPSTDSMMKKLGK
                     SYQYRIYPGVGHGFIRSRDLPAVTDTAWSNIVDFFRAKLGR"
     gene            complement(3374..4402)
                     /locus_tag="VFU75_11935"
     CDS             complement(3374..4402)
                     /locus_tag="VFU75_11935"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HEU5467595.1"
                     /translation="MIAAFALLMLQHPVQVAGHTLTVPTGFDIAVYADSLRGIRYMTL
                     GPHKQVYASIPRDGRVVRFENGKAVNYATGLNQPFGLAWRGDTLYVGDQDALLRIDPD
                     GKKTQLVDLPSGGNHVTRTVLIKGDHIYVSAGSTCNICDESDPRRAAVSIYDLDGKHG
                     RVFASGLRNSVGLAFNPTTGELWGTNNDRDNLGDDLPPEHLNIIKDGKWYGWPKCYLP
                     GKNNPEYPDAKCDTVEPPALTFQAHSAPLGLAFYGGSQFPADYKGDAFATFHGSWNRS
                     TPTGDKVIRIRVKDGKPVSSEDFVTGWQLPDGTRWGRVVGLLVLPDGSLLISDDAGGR
                     VWRVTYGK"
     gene            complement(4399..6012)
                     /locus_tag="VFU75_11940"
     CDS             complement(4399..6012)
                     /locus_tag="VFU75_11940"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HEU5467596.1"
                     /translation="MASLGIWALAPSQSSAQTPGIHVFLYHFELQLPDTGVAFQGSAT
                     VVFRQFPGARDTLALDLIGMHVDSVLNPTLGDAATNQFHYDGHVLTIPIPAAPKTGSV
                     LGRVTVVYHGSPTDGLLAGADSSGHRGLFGDNWPERARYWLPTVDEPSDKAAVDFVVT
                     APAAWKVIANGALVDTASVDGGRMRWTWRERHPIPTYTMVVAAGPLVRSVHPPAGAFT
                     AAVPIDVWTWSGDSAYADSVPFHDVTSVVDVLSRIVGPFPYEKLSHIESATKYGGMEN
                     ASAIFYAERPYTARTMREGVVRHETAHQWFGDAVTEKDFHHVWLSEGFADYFDLVAGA
                     ALHGDSILTAGMQAYKASYLKSNVVDRPLVDTAQSDPNKLLNANSYQKGAWVLHMLRR
                     QVGDSGFFRGVRDYYSTWRDSSVLSAQFQSEIERRVKKNLDWFFKQWLWQPGYPQLTV
                     TWHYEPFKRQAVLDLQQTQDKAWGLFRMDNVPIEFTGPDGKTVRRTVDIDGPPATERF
                     DVPWLPTAIQVDPDGAFLLTSTVTAGAKP"
     gene            complement(6026..7111)
                     /locus_tag="VFU75_11945"
     CDS             complement(6026..7111)
                     /locus_tag="VFU75_11945"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEU5467597.1"
                     /translation="MRRFPAFDPPEYVSWRPDQALAKAFRERLEQDPDRKAVLKSLTK
                     DDHRRLYAGLLRARLHDIQLKRWVRTGVISKAWLGTGEEAATVGPVHALQAGRDIVAP
                     MIRNAAACIEFGIPLTDMFRAYLGSGDSPSGGRDLHVGDFGRGVLQPISHVADVVPVV
                     AGIALAFRIRKEPRVALTWVGDGSTKTAAAHEGTNFAAVQKVPAIFIVQNNQVALGTR
                     VDQHHLPGDFADWAASYGIWGASFDGNNVLDAYAATRVAADRCRSGDGPALLVADTFR
                     LGGHATHDEREARETFPAKLFEDWGRRDPIGQYEGYLMGLGWTEAELSTIEDEVAAEV
                     ERAAEVAAKSRDAMPAGESALGGVYAG"
     gene            complement(7122..8201)
                     /locus_tag="VFU75_11950"
     CDS             complement(7122..8201)
                     /locus_tag="VFU75_11950"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5467598.1"
                     /translation="MSDRRCPRCGSVYSDAARFCPKDGSMLVEVQARPGAPKPAANPA
                     INNTSVRTPAPHAAGGPDRASTLSNQLLDGRYQISKKLGEGGMSYVYLAKELSTGEEV
                     AIKVLSPKLATDKSSVERLRREAGLAMRLDHPNVCKILRLGESEDGLIYLVMPFLKGD
                     LLSDREVQKGPMDLAAGVAILRQVCAGLQHAHELHIVHRDLKPENIMVVTENGADKAV
                     VMDFGLAKERRADPAIQKLTATGIILGTPEFMSPEQIRGKALDARSDIYALGIVAFEL
                     FTGKLPFQGRNAQEMMIARLRGTPTPLRQLRADLPEYLEKALAKAMETNPDSRFSTTM
                     EFADALAGNSAPGGGGGGLISKLFR"
     gene            complement(8198..9637)
                     /locus_tag="VFU75_11955"
     CDS             complement(8198..9637)
                     /locus_tag="VFU75_11955"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HEU5467599.1"
                     /translation="MSDAQPSRILVADDDASLVRTLTWILKEHGYQVSTAPGGEGLLG
                     RLEEERPDLLLLDIMMPKIDGLQLLERLKGDERYRDLPVLMVSSMPPEEATVKSLGLG
                     AADFISKPFRVRELLARVEAHLRVGKALAAAREEARSRAAMVDILHEVTDSLKPDEIY
                     HILARRVARVLNISKCSMVLAKAGDQVGVVVAAYENPMLRNLQIDLARYPEIRKALST
                     GRPVLVADVATDPLYESERTRWEREGIKVPTRSAVALPFSMKDQQIGVFFLRTTGDDL
                     PLTRADAQFAETVIETAVAAIEKAYDFETAVSDKQRLEKLAATDALTGTMNRRALAEA
                     LEAELDRARRYKLGISILLADIDRFKQINDTRGHLAGDSVLRQVGEILRREARSVDIV
                     ARYGGEEFVVVMPETGLQGATIFAERLRQRIEKHDFADTGDQPLNLTVSIGLAAFPDD
                     KVQSAEGFVAVADQALYRAKNEGRNLVRP"
     gene            complement(9645..10574)
                     /locus_tag="VFU75_11960"
     CDS             complement(9645..10574)
                     /locus_tag="VFU75_11960"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine dehydratase"
                     /protein_id="HEU5467600.1"
                     /translation="MVTAEGVREAARGLHGVAVRTPLIWVEPLKVYLKLESLQPVGAF
                     KARGAFTAVRRLSEADRNRGVITYSSGNHGQAVAWAANRFGVRAVIVMPETAPAVKVE
                     GVKKWGGEVVFAGRTSADRHAKAEALVAEQHLAMVPPFDHPDVIAGQATVALEIVEDL
                     PDVETVVVPVGGGGLIAGVVTGLAAAGSAARVWGVEPVGAPKLTKSLAAGAPVKLDKT
                     ASLADGLITLTVGATPFAHLMAQRDRIAGVALVEDDSLREAVHFLHRECRLTVEPSGA
                     ATTAALRAGAIRPVGTTVLVVSGGNVDPKLLED"
     gene            complement(10574..13126)
                     /gene="gyrA"
                     /locus_tag="VFU75_11965"
     CDS             complement(10574..13126)
                     /gene="gyrA"
                     /locus_tag="VFU75_11965"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012681973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEU5467601.1"
                     /translation="MTGPNARERILPRLIEDELKESFLDYSMSVIVQRALPDVRDGLK
                     PVHRRILYAMHELGLVPGRPYKKSATVVGDVLGKYHPHGDISVYDALVRMVQEFSLRY
                     PLVDGQGNFGSVDGDPAAAYRYTEARLTRIAMTMLEDIDKNTVEFIPNFDDRLQEPTV
                     LPAKIPNLIVNGAAGIAVGMATNIPPHNLGEVVQAIQHLVDHPDATVKDLRKFVRGPD
                     FPTGAIIYGREGIKDCYEKGRGRIVVRARAAIEEKQSGRQQIVVTEIPYQVNKANLIT
                     QIAGLAIDKKVEGISNVEDHSDREGMRIVIELKRDAVPAVVLNQLYKYTTMQTTFGAI
                     MLALVDGVPKELTLKELLQSFIDHRHTVITRRTQFELQRAQDREHILEGLKIAVDNID
                     EVIKIIRKSKDTATADAALRKRFKLSERQSAEILNMRLARLTALEINKLEEELKDVRK
                     FIKECKEILASKPRRMKILKEELGEVSHGFSDARRTEIVADQGEFSIEDLIAEEDMVI
                     TVSHSGYIKRLPVSAYRRQRRGGKGITAAHTKEDDWVEHLFIASTHDYVMFFSTSGHC
                     YWLKVHEIPQAARAARGKPVTSCIAMHQDERTASLVPVREFSDDQFLLFATKKGIVKK
                     TVLSEFGNPRTAGIRAINIESGDELIDVQVTDGRNDVVLATGHGMSIRFHEQDVRDMG
                     RAATGVKGIELDKKDHVIDMVIVRRASTLFAITEKGMGKRSELDEYRVQHRGGRGIIT
                     LKHTDKTGDIIALKEVLPDDEMMLITKKGIIIRVPVEGIRISGRNTQGVKVMNLTAGD
                     LVVDVARVVKEDEAAGDAGDDGDDEPETPAPKGKEAPKATEAPKAKPKGKKT"
BASE COUNT         2121 a         4469 c         4352 g         2318 t
ORIGIN      
        1 ccatctccgg cggcacgaat acccgaatcg gcacccccac caccccgatc ccccggctca
       61 catacaactg actcgcgcca tcctggaagt gccccatgac gtacttggtg atgagcctcc
      121 cggccgacca ccccaccacc ggcagataga actgaccgcc atggatgtgg cccgacaacg
      181 tcagcgggat gttgtgcagc ttcgccgcgt cccacgcccc gggccggtgt gccatgagga
      241 tcttgaacgt gtccggcgga gcttcgcggt cgaccctgct cacctccgcg gcttgcggat
      301 cgtcgaagcg ccaatcgagc gggtcgtcga cgccgaggat ggcgagcgtc gcgccgttgc
      361 gctcgatgag atgatgcccg gaccgcacca gcgtctgccc ggcgtcctgc acgcctttgc
      421 tgacctcggc cgctcccgtg tagtggtcgt ggttcccgag ccccgtgatc acgcccagcg
      481 gagccgagag ctcccggaat gcgcggacgt agtccggaat gaaggacggg gagatgtcga
      541 tcatgtcccc ggtctggagg atcagatcgg gggcgagcgc gttcgcctgc gccaccagcc
      601 gctgcatctt ttcgagccgc atgtggatgc cggcgtgcac gtccgacagg tgcaggatct
      661 tcagcccgtc gagcccgggc ggcaggtggt cgaagtagag ggtccgtcgc gtgatctccg
      721 ggagccgcgc gccctcccac agtccgtacg cggacaacgc cagcccggcg ccggcgtacg
      781 cgtaggtccc gcggacgaga aactggcgac gggtgacttg ggagccggga gtcgggagcc
      841 gttcactccg tcgcagtccc cgaacgcaca gcacaacgcc cttgaacagc acgtacaccg
      901 ccaccaggcc gaggaagatc cagcccagca tctgccacgc gacccacggc ccgatgtacg
      961 ggatccagcc cttcgaccac accgacccga acagcacccg ccgggcgacg aaaatccacg
     1021 ggaggttgaa aaacgcgaag agcagaatca gcgaccagcg cacgatgtgt gcctggcgcg
     1081 ggcgcgaccg gtccggcagg cggtcccgca aatacacgta cagcaccgcc tgcggcgcga
     1141 tgaacaccaa gagatagatt aaggcgatgc cgtcacgcat cccgctcttg ctccttgcgg
     1201 ccgccccggc cgtcctgggg gtcaggaccg ctacgggtca gcaactctcc gccaccgatc
     1261 gcgcgcgcat ggtcgccacg ctgtggtccg acgcgcgcta caactacgcc tactgggacc
     1321 gcgtcgaagc cgactgggac tccgcactcg acgccaatct cgctttcgcc gctcagcgcc
     1381 agtccgacgt cacgttctgg cgccgtcttg aggtcctcgc cgcccttttg aacgacggtc
     1441 aggccgccgt cgttccaccc gtcgcggtgc gttcccggac ggcccggcca ccgatccgga
     1501 tccgggcggt agaaggcagg cctttcgtcg ccgactacgc cgagaacgac gagctgcgga
     1561 tcgcgcgccc cggccggggt ttcgaaatcg tttccgtgca gggcgtcctt gcggcgacct
     1621 ggatcaggga ctccatcctg cccggcatcc cgggatccac cgccgcgacc cgctgggagc
     1681 gcgccgtcgc ctccatgctg gtcggagagc gcggcacccc cgtgcagctt gccctcaagg
     1741 gaccgggagg cgtcgaccgg ggcgcctccc tgactcgcag cgtcgcgact tcggcgcgct
     1801 cacccttcga gccgccgccg ctggctgtcg acaccgtcag cggagggatc agcgtcgtgc
     1861 atctcaattc ctgcgccggc gctggcgtcg tctcgcagtt cgaccgcacg ttcaccacct
     1921 ggaccggcgt gcgggctctg atcctcgacc tccgcgagaa tgacggcacg ggtggcgggc
     1981 gcgagtcatg ctaccgcatc ctcgcgcgcc tcatgtccca cccgttcgtc acctcccgct
     2041 ggcgtacccc ggagtatcgc ccagcgtacc gcggcgccga catgcccgac tcggcgggcg
     2101 cctggttctc agcccctccc gataccatcg tgccccgccc cgacctgccc gtcttcaatg
     2161 ggccggtagc cttgctgtcg tccgcgcgca ccgcgggtgc gggtgccggc gccgatctgc
     2221 tcgtggcgtt ccggaacgcg caacggggac cgatcgtggg ggagccatcg gcggggggga
     2281 cggggcaggt gctgaccctg cctctgtccc ggggatggtt cttccgggtc accgtgacgc
     2341 gcgacgcctt ccccgacggc agtgaattcg ccgccaccgg catcgcgccg gagatggccg
     2401 tcacggagac ggtcgccgac tttcagagtg ggaaggacgc agtgctggag cgtgcggtcg
     2461 cgtatctcag ggagcgcacc tcccggtagg ttcccaccct aaaagggtgg gcccgaagga
     2521 cctggcgctt taagcgccgc gcggtgtggg gcccaggctt tgagcctggg tcgacgcccg
     2581 cgtgtggggt gaccctctcc gcccctagcg ccccagcttc gcccggaaaa agtcgacgat
     2641 gttcgaccag gccgtatccg tcaccgccgg aagatcgcgg gagcggatga agccgtggcc
     2701 gactccggga tagatccggt actggtagga cttcccgagc ttcttcatca tcgaatcggt
     2761 cgacggaacc gacaacgaaa tccgcgcgtc cttttccgcg tagacgccga gccccggagc
     2821 cttcaatcgc gcgagggacg cggaatcggg tccgggaccg taacagacga cgaacccctt
     2881 gagctgcgga ttgttggtgg cgtaccggaa gctctgccca ccaccccagc agaacccgat
     2941 gaccccgatc cggtcggcgc gcaccgcctt cacgtgcgcg agaaactggt atgtcgcgtc
     3001 gaggtcctgc gtcagcgtgt cgggattgag gccgcggatc atctggatcg cctgatcctg
     3061 gctggccggc gtgccgcccc gccgggagag aaggtcgggc gcgatggcga cgaaaccgtc
     3121 cttcgccagt tgcgtcaccc ggtccttgac gaaatcggac atcccgaaga tctcgtgaat
     3181 cacgatcacg cccggtgccg cgtcactccg ctccgggtac gcgaggtagg ccgtgatcga
     3241 gtccttcccc gacaggtact tcacgtactc ggaatgaatg tcgacgggct ggggacgcgg
     3301 cgtcgcgagc cgccatgcgg gcaatgcgac ggccagaagc gcgacggcgc cgaggctcag
     3361 cttttccagg cgatcacttg ccataggtca ctctccagac gcggccaccc gcgtcgtcag
     3421 agatgagcag tgaaccatcg ggaagcacga gcaatcctac cacccgcccc cagcgcgtgc
     3481 cgtcaggaag ctgccagccc gtcacgaagt cctcgctcga caccggcttc ccgtccttca
     3541 cgcggatccg gatgaccttg tcgccggtcg gcgtggagcg attccaggag ccgtggaacg
     3601 tcgcgaaggc gtcccccttg tagtcggccg ggaactgcga gcctccatag aaggccaggc
     3661 cgaggggcgc cgagtgcgcc tggaaggtga gggccggcgg ctcgacggtg tcgcacttgg
     3721 cgtccggata ttcggggttg ttcttgccgg ggagatagca cttgggccaa ccgtaccact
     3781 tcccatcctt gatgatgttc agatgctcgg ggggaaggtc gtcgccaagg ttgtcccggt
     3841 cattgttggt gccccagagc tctccggtcg tcgggttgaa cgcgagaccg accgaattcc
     3901 ggagtcccga cgcgaacacc cgaccatgtt tgccgtcgag gtcgtagatg ctcaccgcgg
     3961 cgcgccgcgg gtccgattcg tcgcagatgt tgcaggtgga cccggcggac acgtagatgt
     4021 ggtcaccctt gatcagcacg gtccgtgtca cgtggttgcc gccactcggc aggtcgacca
     4081 gctgcgtctt cttgccgtcc ggatcgatcc ggagcaacgc gtcctgatct cctacgtaca
     4141 gcgtgtcgcc gcgccaggcg aggccgaagg gctggttcaa gcccgtcgca taattgacgg
     4201 cctttccgtt ctcgaagcgc accacccggc catcgcgggg aatcgacgcg tagacctgct
     4261 tgtgtggtcc gagtgtcatg tagcggatgc cgcgcaggct gtcggcgtac acggcgatgt
     4321 cgaaaccggt gggaaccgtg agcgtgtgcc cggccacctg aacggggtgc tgcagcatca
     4381 gcaaggcgaa agcggcgatc atggtttggc ccctgcggta acggtcgaag tcagaaggaa
     4441 cgccccatcc gggtctactt gtatcgcggt ggggagccac ggcacatcga agcgttcggt
     4501 agccggcggc ccgtcgatgt ccacggtgcg ccgaacggtc ttgccgtccg ggcccgtgaa
     4561 ctcgatgggg acgttgtcca tgcggaacag gccccaggcc ttgtcctgcg tctgctggag
     4621 gtcgaggacc gcctgacgct tgaaaggctc atagtgccag gtcaccgtca gctgcggata
     4681 cccgggctgc cagagccatt gcttgaagaa ccaatcgagg ttcttcttga cccgcctttc
     4741 gatctccgac tggaactgtg ccgacagcac gctcgaatca cgccacgtgc tgtagtagtc
     4801 acggaccccg cggaagaacc ccgagtcccc gacttgccgc cgtagcatgt gcaggaccca
     4861 ggcccccttc tggtagctgt tggcgttcag cagcttgttc ggatcggact gcgcggtgtc
     4921 gaccaggggc cggtccacca cattggactt cagatagctc gccttgtagg cctgcatgcc
     4981 ggcggtgagg attgaatcgc cgtgcagcgc ggctcccgcc accagatcga agtaatcggc
     5041 aaagccctcc gagagccaca catgatggaa gtccttctcg gtcactgcgt cgccgaacca
     5101 ctgatgcgcc gtttcatgac ggaccacgcc ttcgcgcatc gttcgagccg tgtacggccg
     5161 ctcggcatag aagatcgcgg aggcattctc catgccgccg tatttcgtcg ccgactcgat
     5221 gtgcgacagc ttctcgtacg gaaagggtcc gacgatccgg gagagcacgt cgacgaccga
     5281 cgtgacgtcg tggaatggca ccgagtcggc gtacgccgag tcgccggacc aggtccagac
     5341 gtcgatgggg acagccgcag tgaacgcgcc ggccggagga tggacgctcc ggaccagtgg
     5401 gccggcagcc accaccatcg tgtaggtcgg gatcggatgc cgctcccgcc aggtccagcg
     5461 catgcgcccg ccgtccaccg aggcagtgtc caccagcgca ccgttggcga tcactttcca
     5521 ggcagcggga gccgtcacca cgaagtcgac cgccgccttg tcactcggct cgtccaccgt
     5581 cggtaaccaa taccgggcgc gctcgggcca gttgtccccg aagagcccgc gatggccgct
     5641 cgagtccgca ccggccaaca gcccgtcggt cggcgacccg tgatagacga ccgtgacccg
     5701 gccgagcacg gaaccggtct tcggcgccgc cgggatgggg atcgtcagga cgtggccgtc
     5761 gtaatggaac tgattggtcg cggcatcacc gagggtcgga ttcagcacgg aatccacgtg
     5821 catgccgatc aagtcgaggg ccagggtatc ccgtgcgccc gggaactggc ggaagaccac
     5881 cgtcgcgctg ccctgaaacg ccacgccggt gtccgggagc tgcagctcga agtgataaag
     5941 gaagacgtgg atgccgggtg tttgcgccga actctgagac ggagcgagtg cccagattcc
     6001 cagtgatgcc actgcggcga tcaacctacc ccgcatacac cccccccaga gccgattccc
     6061 ccgccggcat ggcatcccgg ctcttcgcgg ccacctcggc cgcgcgctcg acctccgcag
     6121 cgacttcgtc ctcgatcgtg ctaagctccg cttcggtcca cccgaggccc atcagatacc
     6181 cctcgtattg cccgatgggg tcacgtcggc cccaatcctc gaacagcttg gccggaaacg
     6241 tctcccgggc ctcgcgctca tcgtgggttg catggcctcc gagccggaac gtatcggcga
     6301 ccaggagcgc ggggccgtcg ccggagcggc accggtcagc ggcgacccgg gtggcggcat
     6361 aggcgtccag aacgttgttg ccgtcgaagc tcgcgcccca gatgccgtag gaagcggccc
     6421 aatcggcgaa atcgccgggg agatggtgct ggtcgacgcg tgtacccagc gccacctgat
     6481 tgttctggac gatgaagatg gcgggcactt tctgcacggc cgcgaaattg gtaccctcat
     6541 gcgccgccgc ggtcttcgtg gagccgtcgc ccacccatgt cagcgcgacc cgtggttcct
     6601 tccggatacg gaaggcgagc gcgatcccgg ccactaccgg tacgacgtcc gcgacgtgag
     6661 agatgggctg aagcacgccc cgcccgaagt ctcctacgtg gaggtcccgc ccgccgctgg
     6721 gtgaatcacc gctcccgaga taggcgcgga acatgtcggt cagcgggatt ccgaactcga
     6781 tgcaggccgc ggcgttccgg atcatcggcg cgacgatatc ccggcccgcc tggagagcat
     6841 ggaccggtcc gacggtcgcc gcctcctctc cggtgccgag ccaggctttg gaaatcacgc
     6901 ccgtgcggac ccagcgtttc agttggatgt cgtgaagccg cgcccggagg agccccgcgt
     6961 agagacgccg gtgatcgtct ttggtcagag acttgaggac tgctttgcgg tcgggatcct
     7021 gttccagacg ttcacggaag gccttggcca gggcctggtc gggccgccag gagacgtact
     7081 ccggagggtc gaatgcggga aagcggcgca tcgagagaag actagcggaa cagcttcgaa
     7141 atcagtcccc ccccgccgcc cccgggggca ctgttgccgg ccagcgcgtc cgcgaactcc
     7201 atggtggtgg agaagcggct gtcggggttc gtctccatcg ccttggcgag cgccttctcg
     7261 agatactcgg gaaggtcggc gcgcagctgc cgcaggggcg tgggggtgcc ccgcaaccgc
     7321 gcgatcatca tctcctgcgc gttgcgtccc tgaaacggga gttttccggt gaacagctcg
     7381 aaggccacga tgccgagcgc gtagatgtcg gaccgcgcgt cgagtgcctt gccgcggatc
     7441 tgctcggggc tcatgaactc gggggtgccg agaatgatgc cggtcgccgt gagcttctga
     7501 atggccggat ccgcccgccg ctccttcgcg agcccgaaat ccatgaccac ggccttgtcc
     7561 gcgccattct ccgtgacgac catgatgttt tcgggcttca ggtcgcgatg gacgatgtgc
     7621 agctcatgtg cgtgctggag ccccgcgcag acctgccgga ggatcgcgac gccggcggcc
     7681 agatccatcg gccccttctg cacttcgcgg tcggaaagga ggtcgccctt gaggaagggc
     7741 ataaccagat agatgaggcc gtcctccgac tcacccagcc gcaggatctt gcagacgttg
     7801 ggatggtcga ggcgcatggc gaggcccgct tcgcgccgca gccgctccac cgagctcttg
     7861 tcggtggcga gcttgggcga gaggaccttg atggccacct cttctcccgt tgacagctcc
     7921 ttggcgagat agacgtacga catcccgccc tcgccgagct tcttggaaat ctggtaacgc
     7981 ccgtcgagca gctggttgga gagcgtcgat gcccggtcgg ggccgcccgc ggcatgcggg
     8041 gccggcgtac ggaccgacgt gttgttgatc gcggggttcg cggcgggttt aggcgcgccg
     8101 gggcgggcct gcacctccac cagcatgctt ccatccttcg ggcagaagcg tgcggcatcg
     8161 gagtacacgg acccacagcg gggacagcgg cggtcactca tgggcggacc aagttgcgcc
     8221 cttcgttctt ggcgcgatag agggcctggt cggccaccgc cacgaatcct tcggcgctct
     8281 ggaccttgtc gtcgggaaat gcggccagcc cgatcgaaac cgtgaggttc aggggctgat
     8341 cgccggtgtc ggcgaaatcg tgcttctcga tccgctgccg gaggcgctcg gcgaagatcg
     8401 tcgcgccctg cagcccggtc tcgggcatca cgacgacgaa ctcctcaccc ccgtaccgcg
     8461 ccacgatgtc cacggatcgt gcttcgcggc gcaggatctc accgacctgc cggagcaccg
     8521 agtctcccgc caggtggccg cgcgtgtcgt tgatctgctt gaaccggtcg atgtccgcca
     8581 gcaggatgct gatgcccagt ttgtagcgcc gggcccggtc caactccgcc tcgagcgcct
     8641 cggccagggc acggcggttc atcgtgcccg tgagtgcgtc ggtcgcggcg agcttctcga
     8701 gccgctgctt gtccgagacc gcggtctcga aatcgtacgc cttctcgatg gcggccacgg
     8761 cggtctcgat caccgtctcg gcgaactgcg cgtcggcgcg cgtcagaggg agatcgtccc
     8821 cggtggtccg caggaaaaag acgccgatct gctggtcttt catcgagaag ggcagggcca
     8881 ccgccgaacg cgtggggacc ttgatgccct cgcgctccca gcgggtgcgc tccgattcat
     8941 agagcgggtc ggtggccaca tcggccacga ggaccggacg tccggtactg agcgccttgc
     9001 ggatttccgg gtagcgcgcg aggtcgatct ggaggttccg cagcatcggg ttctcgtacg
     9061 cggccaccac gaccccgacc tggtcgccgg ccttggccag caccatcgag cacttggaga
     9121 tgttgagcac ccgcgccacc cggcgggcga ggatgtgata gatctcgtcg ggcttgagcg
     9181 agtcggtgac ttcgtggagg atgtccacca tcgccgcgcg tgaccgcgcc tcttcgcgcg
     9241 ccgccgccag cgccttcccc acccggaggt gcgcttccac ccgggccagg agctcgcgga
     9301 cgcggaacgg cttggaaatg aagtccgcgg cgccgaggcc aagggatttg acggtggctt
     9361 cttcgggggg catcgaggag accatcagca ccggcagatc gcggtagcgc tcgtcgccct
     9421 tgaggcgctc gagcagctgc agcccgtcga tcttgggcat catgatgtcg agcaggagga
     9481 ggtcggggcg ctcttcctcc aggcgcccca gaagcccttc accgccgggc gccgtcgaaa
     9541 cctggtaccc gtgctccttc aggatccacg tgagcgtccg gacgagcgac gcgtcgtcgt
     9601 ccgccacgag tatccgggag ggttgggcgt ccgacatcct gggctcagtc ctcgagaagc
     9661 ttgggatcca cgttgccacc gctgacgacc agcacggtcg tgcccaccgg gcggatggcg
     9721 ccagcgcgaa gggccgcggt ggtcgccgca cctgacggct ccacggtcag ccggcactcg
     9781 cgatgtagaa aatgcaccgc ctcccgaagg ctgtcatcct ccacgagggc gacgcccgcg
     9841 atgcggtcac gctgggccat caggtgcgcg aagggggtcg cgcccaccgt cagcgtgatc
     9901 aacccgtccg ccagactcgc cgtcttgtcc agcttcaccg gggcccccgc cgcgagggac
     9961 ttcgtcagct tcggcgctcc cacgggctct acgccccaga cccgcgcggc gctgcccgcc
    10021 gccgcgagcc cggtcacgac cccggcgatc agccctccgc cgccgacggg caccacgacc
    10081 gtttcgacgt cagggagatc ttcgacgatt tcgagggcga cggtggcctg acccgcgatg
    10141 acgtcggggt gatcgaaggg cgggaccatg gcgagatgct gctccgccac cagcgcttcg
    10201 gcctttgcgt ggcggtcggc ggaggtccgc cccgcgaaga cgacctcccc accccatttc
    10261 ttcacgcctt cgaccttcac cgcgggcgcg gtttccggca tcacgatcac ggcgcgcacg
    10321 ccgaaccggt tggcggccca ggcgacggcc tgtccgtgat ttcccgagga gtacgtgatg
    10381 accccgcggt tccggtctgc ctcggagagg cggcgcaccg cggtgaatgc gccgcgggcc
    10441 ttgaaggccc ccacgggctg caggctttcg agcttgaggt agaccttgag cggctcgacc
    10501 cagatcaagg gagtccggac cgccacgcca tggagcccgc gcgcggcttc tcgaacccct
    10561 tcggcggtca ccatcacgtc ttcttgcctt tgggcttggc cttgggcgct tccgtggcct
    10621 tgggcgcttc cttgcccttt ggagcggggg tctcgggttc gtcgtctccg tcgtccccgg
    10681 cgtctcccgc cgcttcgtct tccttcacca cccgcgcgac gtcgaccacc agatctccgg
    10741 cggtcaggtt catcaccttc acgccttgcg tgttgcggcc cgagatccgg atgccctcca
    10801 ccgggacccg gatgatgatc cccttcttgg tgatcaacat catctcgtcg tccggcagga
    10861 cctctttcag cgcgatgatg tccccggtct tgtccgtgtg cttgagggtg atgatgcccc
    10921 ggccaccgcg gtgctggacg cggtactcgt cgagctccga gcgtttgccc atgcccttct
    10981 cggtgatggc aaagagcgtc gaggcccgcc ggacgatcac catgtcgatg acgtggtcct
    11041 tcttgtccag ctcgatgccc ttcacgcccg tcgccgcccg gcccatgtcg cggacgtcct
    11101 gctcgtggaa ccggatgctc atgccgtggc cggtggccag caccacgtcg ttccggccgt
    11161 cggtcacctg cacgtcgatc agctcgtccc cgctctcgat gttgatggcc cggatacccg
    11221 cggtgcgggg gttcccgaac tccgagagca ccgtcttctt gacgatgccc ttcttggtgg
    11281 cgaagagcag gaactgatcg tccgagaatt cccggaccgg gacgagagac gcggtgcgtt
    11341 cgtcctggtg catcgcgatg caggacgtga ccggctttcc gcgcgccgcg cgcgcggctt
    11401 gcgggatctc atgcaccttc agccagtagc agtggccgga ggtgctgaag aacatcacgt
    11461 agtcgtgggt cgacgcgatg aacaggtgct cgacccagtc gtcttccttc gtatgcgcgg
    11521 ccgtgatgcc cttgccgccc cggcgctggc gccggtaggc ggacaccggg agccgcttga
    11581 tgtagcccga gtgcgagacc gtgatcacca tgtcttcttc ggcgatcagg tcttcgatgg
    11641 agaactcgcc ctggtcggcg acgatctcgg tccggcgcgc gtcgctgaag ccatgggaca
    11701 cctcgccgag ctcttccttg aggatcttca tccggcgggg cttggacgcc aggatctcct
    11761 tgcactcctt gatgaacttg cggacgtcct tcagctcctc ctcgagcttg ttgatctcga
    11821 gggccgtgag ccgggcgagc cgcatgttga ggatttcggc cgactgccgc tccgacagct
    11881 tgaagcgctt gcgcagggcg gcgtccgccg tggccgtgtc cttcgacttc cggatgatct
    11941 tgatgacctc gtcgatgttg tcgacggcga tcttcaaccc ttcgaggatg tgctcccggt
    12001 cctgggcccg ctgcagctcg aactgcgtcc ggcgggtgat gacggtgtgg cggtggtcga
    12061 tgaaactctg cagcagctcc ttcagcgtca gttccttcgg gacgccgtcc accagcgcca
    12121 gcatgatggc gccgaaggtc gtctgcatcg tggtgtactt gtagagctgg ttcagcacca
    12181 cggcgggcac cgcgtcgcgt ttcagctcga tcacgatccg catgccttcg cggtccgagt
    12241 gatcttcgac gttcgagatg ccctcgacct tcttgtcgat cgccaacccg gcgatctgcg
    12301 tgatcaggtt cgccttgttg acctgatacg gaatctccgt gaccacgatc tgctgccggc
    12361 ccgactgctt ctcttcgatg gcggcgcggg cgcggaccac gatgcggccg cggcccttct
    12421 cgtagcagtc cttgatcccc tcccggccgt aaatgatcgc gccggtcgga aagtcgggac
    12481 cgcggacgaa tttccggaga tccttgaccg tggcgtcggg atggtcgacc aggtgttgaa
    12541 tggcctgcac cacctcgccg aggttgtgcg gcgggatgtt ggtcgccatg cctaccgcga
    12601 tgccggcggc gccattgacg atgagattcg ggatcttggc cgggagaacg gtcggttcct
    12661 gcaggcggtc gtcgaagttg gggatgaact cgacggtgtt cttgtcgatg tcctccagca
    12721 tcgtcatcgc gatccgcgtc agccgcgctt ccgtgtaccg gtacgcggcc gccggatcgc
    12781 cgtcgacgga accgaagttc ccctgcccgt ccaccagcgg ataccgcagc gagaactcct
    12841 ggaccatgcg gaccagggcg tcgtagacgg agatgtcgcc gtgcgggtga tacttgccga
    12901 ggacgtcgcc gacgaccgtg gcgctcttct tgtacggccg tcccggcacc aggccgagct
    12961 cgtgcatcgc gtagagaatc cggcggtgca ccggtttcag tccgtcgcgc acatcgggca
    13021 gcgcgcgctg gacgatcacg ctcatcgaat agtcgaggaa ggattccttc agctcgtctt
    13081 cgatgaggcg cggaagaatg cgttcgcggg cgttgggtcc ggtcatgaaa ggcaggtctc
    13141 aggggtcagg agtcaggggc cagggaaatg ccctaagtcc aaaaaatcta aatacttacg
    13201 tggtcaaaag caacgaaaaa atgaccccta aaccggggcg ggaaaggcac ttacgggagg
//
LOCUS       DASUBP010000314         4876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_708343, whole genome shotgun sequence.
ACCESSION   DASUBP010000314 DASUBP010000000
VERSION     DASUBP010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708343"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="VFU75_11970"
     CDS             complement(<1..374)
                     /locus_tag="VFU75_11970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467602.1"
                     /translation="MRRVTTWCVLMVAGVNALAAQDSQFGIRGLGAPGTFASVRAWAT
                     GGAFAPFDGMSAVADASLTEVVRLTAAATEVSDYRTVNDGTNSSSLRSSRFPMLQVAG
                     PVVGHLVVGGGFSTYLYRSYSVR"
     gene            complement(374..1660)
                     /locus_tag="VFU75_11975"
     CDS             complement(374..1660)
                     /locus_tag="VFU75_11975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467603.1"
                     /translation="MRIGRRTLLALGMAATLAACTEDATAPGKCPAFCPPGQVQVVDT
                     ILHVISRDSSFTGYVQPDSAQVMLAANAFGIDSRPIFVMAPVGTRVLLNPNGSDTTTV
                     APTSIDSATLVLVIDRRDTLASNLRISLYRMPLNIDRNSTLSGLAGPFTDSLIRTMNV
                     DSVFKLPAGVDSTAGDSVAAVDSSRIPVKVKITLDSAQARFIAADSGRSAFGIRVTAD
                     SLASVAFESHGPVLTWWATYDSSGTAVHRLMAIADAVFSSFVSNAASPTLDSNLVIGG
                     VPSARALLRFAIPRAIRDSAQVVRATLYLVPVTAPVGVPSDSVVITFSRVSTDIGAKS
                     PCVPSALGSGCLPLGDSAFVVSASAPMGQTDTIATDITQIVRGWAVDTVAPQTLMVRQ
                     LPEGGNLVYLRVYSSRSAFRPALHITYVPRFSFGNP"
     gene            complement(1662..2066)
                     /locus_tag="VFU75_11980"
     CDS             complement(1662..2066)
                     /locus_tag="VFU75_11980"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: protein motif:HMM:NF004064.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009972 - cytidine deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEU5467604.1"
                     /translation="MTADAAVTELVRRAREAMRNAHAPYSRFHVGAAIEAGDGTIYVG
                     CNVESASYGLTICAERMALGAAVAGGATRIVRVVVTTEVDPPAAPCGACRQLLAEFGL
                     TMEIRAVGPASERRWTLGALLPDAFTRETLEQ"
     gene            complement(2063..3232)
                     /locus_tag="VFU75_11985"
     CDS             complement(2063..3232)
                     /locus_tag="VFU75_11985"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012682939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEU5467605.1"
                     /translation="MTTRRAGIIVLDGLGIGTAPTTEAYGDLGSDTLGNVARAVGGLT
                     LPNLERLGLGLCRPLTGIGRPKSPGAAYGVALPASAGKDSTTGHWEICGVLLERPFRT
                     YPQGFPAALLDEFSKRTGRGWLGNKAASGTAIIAELGAEHQRTGQWIIYTSADSVFQV
                     AAHEETVPLEELYRACRVARELLVGEEAVSRVIARPFKGASPAYERTANRRDFSIEPP
                     QATLLDRLADWGVPRVGVGKVDDLFAGRNITSVHTPTNPDAYRLIERALETMDSGLLF
                     VNVIEFDQSWGHRNDVAGFHLGLSALDAWIPRFLERLKPSDLMILTADHGNDPTTKST
                     DHSREAVPVLVCGPKIKPVGIGERSTFADLGATVAEHLGLGGIGAGTSFHPQVWA"
     gene            complement(3229..4491)
                     /gene="alr"
                     /locus_tag="VFU75_11990"
     CDS             complement(3229..4491)
                     /gene="alr"
                     /locus_tag="VFU75_11990"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEU5467606.1"
                     /translation="MSAPCPTDNGLAGHGRARGRGRVRRQRRSPWHKHKRFLVLPSGD
                     AVHIIRAPVSSQSHLPSSDRHLQHRAWVEVDFRALVDNARTVAQVSGAPLIPVIKADA
                     YGLGAVGAAKALEVLDPWGYAVATVEEGAALRASGISRRLLVFSPAHPARFPEFDRHQ
                     LTPVFDHLPALLEWRARGDTPFHVEIDTGMSRTGARPEYLGEWRGALDTPAFEGCFTQ
                     FHSAHKDVDATTAQWESLLQAVRAMPRRPAVVHAANSAGALRGKRYAGDAVRPGIFLY
                     GGDPGKGLPEGRPVAHVRSRVLSVRRIAAGDSVGYGATWRAAKATTIATLGIGYADGV
                     RRSVVKTGEAYVLMKGARYPYAGVITMDLTMIDVGDAAVGLGDVATLLGADGGELITL
                     AKHASWCQESQYVILTGLGPRLPRVPVS"
     gene            4401..>4876
                     /locus_tag="VFU75_11995"
     CDS             4401..>4876
                     /locus_tag="VFU75_11995"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG nucleotide pyrophosphohydrolase
                     domain-containing protein"
                     /protein_id="HEU5467607.1"
                     /translation="MANDADASLGRALALVRDLRARCPWDMAQTPESLRPYLVEEALE
                     LDQAIGAGDVPHIQAELGDLLLNLAFQIVIAEERGWFGAENVTRGLEEKMWRRHPHLF
                     GLGEKPKTWEAKKREEGGGRSTLSGLPPTLPRLLMAYRLQERAAGVGFDWPDAKGPA"
BASE COUNT          851 a         1696 c         1597 g          732 t
ORIGIN      
        1 cggaccgaat aggagcgata caggtaggtc gagaagcctc ccccgaccac gaggtggccc
       61 accaccgggc cggcgacctg cagcatcggg aagcgggagc tccgcagcga gctcgagttc
      121 gtcccatcgt tgacggtccg ataatcgctg acctcggtgg cggcggccgt gaggcgcacg
      181 acctccgtca gcgaggcatc ggccacggcc gacattccgt cgaagggcgc gaaggcaccg
      241 cccgtggccc aggcgcgcac gctggcgaac gtcccgggcg ccccgaggcc acgaatgccg
      301 aactgcgagt cctgcgcggc cagggcgttg accccggcca ccatcagcac gcaccaggtg
      361 gtcacgcggc gcatcaagga ttcccgaagg agaaacgggg cacgtaggtg atgtggagcg
      421 cgggccggaa cgcggagcgc gatgaataga ctcgcaggta gacgaggttc ccaccctcgg
      481 ggagctgtcg caccatcagc gtctggggag cgaccgtatc aaccgcccat ccgcggacga
      541 tctgcgtgat gtccgtggcg atggtgtcgg tctgacccat cggtgccgaa gccgacacca
      601 cgaacgccga atctcccaag ggcagacagc cgctccccag cgcgctcggc acgcacggag
      661 acttcgcccc gatgtccgtg ctgacgcgcg aaaacgtgat caccaccgaa tcggacggca
      721 cgccgaccgg ggccgtcacc ggcacgaggt acagcgtcgc ccggaccacc tgcgccgaat
      781 cccggatcgc acgcggaatc gcgaaccgca ggagcgcccg cgccgacggc accccgccga
      841 tcaccaggtt cgagtcgagg gtcggtgaag ccgcgttgct cacgaatgag ctgaagaccg
      901 cgtccgcgat cgccatgagc cgatgcaccg cggtccccga cgagtcatag gtggcccacc
      961 aggtcagcac cggtccatgg gattcgaagg cgacgctcgc cagggaatcg gcagtgacgc
     1021 gaatcccgaa tgcggagcgg ccggagtcgg ccgcgatgaa gcgcgcctgg gcggaatcga
     1081 gcgtgatctt caccttgacg gggatccgcg agctatcgac ggcggcgacc gaatcgcccg
     1141 cggtcgaatc cactccggcc ggcagcttga acacgctgtc gacattcatg gtgcggatca
     1201 gcgaatccgt gaacgggccc gccagaccgc tcagggtcga attccggtcg atgttgagcg
     1261 gcatgcggta gagactgatc cgcagattgc tggccagcgt atcgcgtcga tcgatcacca
     1321 ggaccagcgt cgcggagtcg atcgaggtcg gcgcgaccgt cgtcgtgtcg gagccattgg
     1381 gattcaggag cacgcgcgtg cccacggggg ccatcacgaa aatgggacgg ctgtcgatgc
     1441 cgaaggcgtt cgcggccagc atcacctgag cgctgtcggg ttgtacgtac cccgtgaagc
     1501 tcgaatcccg cgaaatcacg tgcagaatcg tgtccaccac ctggacctgc cccggcgggc
     1561 agaaagcggg acacttcccg ggcgcggtgg cgtcctcggt gcacgccgcc agggtggcgg
     1621 ccatgccgag ggcgagcagg gtccggcgtc cgatcctcaa ctcattgctc cagtgtctcc
     1681 ctcgtgaatg cgtcgggcaa gagtgcgccc agcgtccagc gacgttcgct ggcgggaccc
     1741 accgccctga tctccatggt cagaccgaat tccgccaaca gctgccggca cgcgccgcaa
     1801 ggggccgccg gcgggtcgac ctccgtggtc actaccacgc gaacgatgcg cgtcgcgccg
     1861 cccgccaccg ccgcccccaa cgccatccgc tcggcgcaga tggtcaaccc gtacgatgca
     1921 ctctcgacat tgcagccgac gtaaatcgtg ccatccccgg cttcgatcgc cgcaccgacg
     1981 tgaaacctgc tatagggcgc gtgcgcgttc cgcatcgcct cgcgggcccg gcggaccagc
     2041 tccgtgactg cggcgtcggc cgtcacgccc acacctgcgg gtgaaacgac gtcccggcac
     2101 cgatcccacc taaccccaag tgctccgcca ccgtggcgcc gaggtccgcg aacgtggacc
     2161 gctcaccgat tcccaccggt ttgatcttcg ggccgcacac cagcaccggc accgcctcac
     2221 gcgagtgatc ggtcgacttc gtcgtcggat cgttaccatg atccgccgtc aatatcatca
     2281 ggtcagaggg cttgagccgc tccaggaacc gtgggatcca cgcgtccaga gcggaaagcc
     2341 ccaagtgaaa ccccgccacg tcgtttcggt gcccccagct ctggtcgaac tcgatcacgt
     2401 tcacgaacag gagtccggag tccatcgtct cgagggcccg ttcgatcagc cggtacgcgt
     2461 ccggattggt cggggtgtgg acgctggtga tgttccggcc cgcgaacagg tcgtcgacct
     2521 tcccaacccc tacccgtggg accccccagt cggccaaacg gtcaagcaag gtcgcttggg
     2581 gaggctcaat ggagaagtcc cgccggttgg ccgtccgctc gtaggcgggc gaggccccct
     2641 tgaagggccg cgcgatgacc cggctgacgg cctcctcccc caccaagagc tcccgggcga
     2701 cccggcaggc gcggtacagc tcctccaggg gcaccgtttc ttcatgcgcc gcgacctgaa
     2761 acaccgaatc agctgacgta tagatgatcc actgccccgt tcgctggtgc tccgcaccca
     2821 gctcggcgat gatggcggtc cctgacgcgg ccttgtttcc cagccatccc cggccggtcc
     2881 gcttgctgaa ctcatccagc agcgccgccg ggaagccctg tgggtaggta cggaaggggc
     2941 gctcgaggag caccccgcag atctcccagt ggccggtggt gctgtctttc cccgccgagg
     3001 ccggcaaggc gaccccatac gcggcgcccg gggatttcgg acggccgatc ccggtgagcg
     3061 gccggcagag accgaggccc agtcgctcga ggttcgggag cgtcaggcct cccaccgctc
     3121 tggcgacgtt cccgagggtg tcgctcccca gatcgccgta tgcctccgtc gtcggggcgg
     3181 tcccgatacc cagaccatcg aggacgatga tccccgcgcg acgggtcgtc atgaaacggg
     3241 cactcgcgga agccgggggc ccaagccggt gaggatgacg tactgactct cctggcacca
     3301 ggacgcgtgt ttcgccagcg tgatgagttc tccgccatcc gcgccgagca gcgtggcgac
     3361 atcgcccagc cccaccgcgg cgtcgccgac gtcgatcatc gtcaggtcca tcgtgatgac
     3421 gcccgcgtac ggataccgtg cgcccttcat caggacgtac gcctcacccg tcttcactac
     3481 cgaacgacgt acgccgtccg cgtaaccgat cccgagggtc gcgatggtcg tcgctttcgc
     3541 tgcgcgccac gtggctccgt acccgacact gtcgccggcc gcgatgcgcc gcacactgag
     3601 cactcgcgac cggacgtgcg cgaccggcct cccctccggc agtccttttc cgggatcacc
     3661 accgtagagg aagatcccgg gacgcaccgc gtccccggcg taacgcttgc cgcgcagcgc
     3721 tccggcactg ttcgcggcgt gcaccacggc cgggcgccgg ggcatcgcgc ggaccgcctg
     3781 caacagactc tcccattgcg cggtcgtggc gtcgacatcc ttgtgggccg agtgaaactg
     3841 ggtgaaacag ccctcgaacg cgggagtgtc gagcgctcct cgccactcac cgagatactc
     3901 tggccgcgct cccgtgcggg acattccggt gtcgatctcg acgtggaagg gcgtgtcccc
     3961 gcgcgcgcgc cattcgagca gcgcggggag atgatcgaag actggggtca gttggtggcg
     4021 gtcgaattcc gggaagcgcg cagggtgagc gggcgagaag acgaggagcc ggcggctgat
     4081 cccggaagcg cggagggcgg ccccctcctc caccgtggcg accgcatacc cccacgggtc
     4141 cagcacctcg agcgcctttg ccgcgcccac cgcccctagt ccgtaggcat ccgccttgat
     4201 gaccggaatc agtggcgctc ccgagacctg agcgaccgtc cgggcattat ccaccagcgc
     4261 gcggaagtcc acctccaccc acgcacggtg ttgaaggtgt cgatcgctgg aggggagatg
     4321 gctctgggag ctcacgggcg cgcgaatgat atgaaccgcg tcacctgaag gcaagactaa
     4381 aaagcgctta tgtttatgcc atggcgaacg acgctgacgc ctcactcggc cgcgccctcg
     4441 cgctcgtccg tgacctgcga gcccgttgtc cgtgggacat ggcgcagaca ccggaatccc
     4501 tcaggcccta tctcgtcgag gaagctctcg agctcgacca agccatcggc gcgggtgacg
     4561 ttccccatat ccaggctgaa ctcggtgatc tcctcctcaa cctggcgttc cagatcgtca
     4621 ttgcggaaga gcgtggctgg ttcggggccg agaacgtgac ccggggactc gaagaaaaga
     4681 tgtggcggcg ccacccgcat ttgttcggac tcggcgagaa gccgaagacg tgggaggcaa
     4741 agaagaggga ggagggagga gggaggagta cgctgtcggg gcttcccccg accctgcccc
     4801 ggctcctcat ggcgtaccgg cttcaggagc gcgccgccgg cgtcggattc gattggcctg
     4861 acgcgaaggg accggc
//
LOCUS       DASUBP010000315         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_899424, whole genome shotgun sequence.
ACCESSION   DASUBP010000315 DASUBP010000000
VERSION     DASUBP010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_899424"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1344)
                     /locus_tag="VFU75_12000"
     CDS             complement(<1..1344)
                     /locus_tag="VFU75_12000"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HEU5467608.1"
                     /translation="MRLTGAVLLVAALAACRQPAPAGFTLLFFRSSSTAQVGSLSWAP
                     DLPGGRLVAFDEQLHVTRVVTAGIDQPVAVAVPCGHVPIVSDYSGQVTSFDTAWKTPS
                     EFETHFPASIFASAGCRWIEGRSPYLIEPFSTDTTKGLVVTHDVLEDGGLSQVGALHP
                     AALPFLVGPENAGALALDSTGAFYFAPVARDEITKYNRLGVSRWTARRGLYPQETEPR
                     FLPNRGKEVPLQMARAGIALTLGPDGRLYALGADDSTASKLRVDVLDTATGTILLTRH
                     LGPKETAVALDPHGDLVTFDADSLTSTEQTTPDSRLPFSPLFKLPDLGGDTVRLKQDS
                     GKVTLVNFWASWCDPCREEFPHMASLYGKFSRKDFEIAAISDDVDPARMRAFVAAFHP
                     PFPILVGGGRMKALYHYRGLPYSVLLDRHGRIVKRIFGFGGPQEFQELAATIAKEI"
     gene            complement(1344..1712)
                     /locus_tag="VFU75_12005"
     CDS             complement(1344..1712)
                     /locus_tag="VFU75_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467609.1"
                     /translation="MFRQIFAATAALGLFALPLAAQAPAPGGDVTMTGTVVDVSCYSA
                     LGASGPSHKACAQTCAHSGSPLAIMADGVIYMPVSPKPNNPQNPRLVNFAEGKVKVTG
                     TVKEVAGIHTIVIKTVEAAS"
     gene            1757..2143
                     /locus_tag="VFU75_12010"
     CDS             1757..2143
                     /locus_tag="VFU75_12010"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5467610.1"
                     /translation="MPDNYRALLETFGSGWERGDAEAICSVFAPDAVFIETPFSTPDR
                     GIQAIRAYWTEVPTHQAEITFKAGEIYVAGPWFSTEFQCKFRRRRTGERVDARGAIFC
                     ETKGGKITEMRMYWHRYSGNREDSKP"
     gene            complement(2168..>2384)
                     /locus_tag="VFU75_12015"
     CDS             complement(2168..>2384)
                     /locus_tag="VFU75_12015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467611.1"
                     /translation="KVREQVRNLVGKKPTFCCCNSGNRVGAALLPYLMLDRGMDEDEA
                     TMVAMKIGMRNAELMESALDYVRRQAR"
BASE COUNT          455 a          723 c          787 g          419 t
ORIGIN      
        1 gatttccttg gcaatggtgg ccgcaagctc ttgaaactcc tgaggcccgc cgaatccgaa
       61 gatgcgcttg acgatcctcc cgtgccggtc gaggagcacc gagtagggca ggccgcggta
      121 gtggtacagc gccttcatcc ggccgccacc gacgaggatc ggaaacgggg gatgaaaggc
      181 cgcgacgaag gctcgcatcc tggcgggatc cacgtcgtcg gagatggcgg caatctcgaa
      241 gtccttccgc gagaacttgc cgtagagcga agccatgtgg gggaactcct cccggcacgg
      301 gtcgcaccag gaggcccaga aattcacgag tgtgaccttg cccgagtcct gcttgagtct
      361 gacggtatcg ccaccgaggt cagggagctt gaatagcggc gaaaacggga gtcgggagtc
      421 gggagtcgtc tgctccgttg acgtgaggct atcggcgtcg aaagtgacca ggtctccgtg
      481 ggggtcgagc gccacagcgg tctccttcgg tccgaggtgt cgcgtcagga gaatcgtccc
      541 cgtcgcggtg tcgaggacgt cgacccggag tttcgaggct gtcgagtcgt cggcgccgag
      601 ggcgtagagc cgtccatcgg gacccagtgt cagagcgatt ccggcccgag ccatctggag
      661 cgggacttcc ttgccccggt tgggaagaaa gcgtggctcg gtttcctgtg gatacagccc
      721 gcgcctcgcg gtccagcggc tcacgcccag gcgattgtac ttggtgatct cgtcgcgagc
      781 gacgggagca aagtagaagg cccccgtcga atccagcgcg agcgctcccg cgttctcggg
      841 gcccaccagg aacggcaggg ccgccggatg cagtgctccg acctggctca acccaccatc
      901 ctcgagcacg tcatgagtga cgaccagccc tttggtcgtg tcggtcgaaa agggctcgat
      961 gagatacggt gacctgcctt cgatccacct gcacccggcg gacgcgaaga tcgacgccgg
     1021 gaagtgcgtc tcgaattcac tcggagtctt ccaggcggtg tcgaaactcg tcacctggcc
     1081 gctgtagtcc gacacgatgg gaacgtgacc gcacggcact gcgactgcga ccggctgatc
     1141 gatgccggcc gtgacgacac gagtgacgtg gagttgttcg tcgaaggcca ccaatcgccc
     1201 ccccggcagg tcgggcgccc aggacaatga accgacttga gctgtggagg acgaacggaa
     1261 gaagaggagt gtgaaaccag cgggggccgg ttgccggcac gcggccagag ccgcgaccag
     1321 cagcacggcc cccgtaaggc gcatcagctt gcggcttcga ccgtcttgat cacgatcgta
     1381 tgaatcccgg cgacttcttt caccgtcccg gtgaccttca ccttgccttc ggcgaagttg
     1441 accagccggg ggttctgcgg attgttcggc ttgggactga cgggcatgta gatcacgccg
     1501 tcggccatga tcgcgagtgg ggaccccgaa tgcgcacacg tctgcgcaca ggccttatgg
     1561 ctcggcccgg acgctcccag agcggagtag cagctgacgt cgacgaccgt gccggtcatc
     1621 gtgacgtccc cgccgggggc cggagcctgg gcagcgaggg ggagagcaaa caggccaagg
     1681 gcggcggttg cagcgaagat ttgcctgaac atgacggacc tccgtgaggg tacagatagg
     1741 actatattct gcggcgatgc ccgacaatta cagagccttg ttagagacct tcgggtccgg
     1801 ctgggaacgt ggcgatgccg aagcgatttg ctcggtgttc gcccccgatg cggtctttat
     1861 cgagacgcca ttcagtaccc ccgaccgggg cattcaggcc attcgggcct actggaccga
     1921 ggtgcccacg catcaggccg agatcacgtt caaggctggg gagatctacg tcgcgggacc
     1981 gtggttctcg acggagtttc agtgcaagtt ccggcgccgc cggacgggcg agagggtcga
     2041 cgcgcgaggc gcgatctttt gtgaaacgaa gggtgggaag atcaccgaaa tgcgcatgta
     2101 ctggcatcgg tattcgggga accgggaaga ttcgaaaccc tgactagacg gttaaacggc
     2161 tagacggcta gcgagcttgc cggcggacgt agtccaacgc actctccatc agctccgcgt
     2221 tcctcattcc aatcttcatc gccaccatcg tggcttcatc ctcgtccatg ccgcggtcga
     2281 gcatcaagta aggaagcaac gccgctccca cccgattgcc ggaattgcag cagcaaaagg
     2341 tcggcttctt gcccaccaga ttccggacct gctcccgaac cttc
//
LOCUS       DASUBP010000316         2415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_722073, whole genome shotgun sequence.
ACCESSION   DASUBP010000316 DASUBP010000000
VERSION     DASUBP010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_722073"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..496
                     /locus_tag="VFU75_12020"
     CDS             <1..496
                     /locus_tag="VFU75_12020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467612.1"
                     /translation="GPAGGQFAAPDRSAVRLMSADQGPNGGAPPATTERLRFQEFTFQ
                     RLPNGRCRAKVQLGRSGATLFSGQADGVTSPAGELRCSAEAAVNALDDAVQAEIEFEL
                     LGVKAVRAFDATVVIVSLSAREESKATRLVGTYLTETDPPRGAALAVLNATNRILGNH
                     FSTR"
     gene            493..2085
                     /locus_tag="VFU75_12025"
     CDS             493..2085
                     /locus_tag="VFU75_12025"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEU5467613.1"
                     /translation="MTVGRASRVFGWMAVSALMAGCGGRATPVATAPTPASAPASVAS
                     AAPTDTAKTKLTGADSVARAQAAVDRDEAADQRVLDTLQQGLPDSTSRIAVATSSIPR
                     EEVRHEAERLFGPDKPTAPSAGPKGGDATFDIDVSTFSGNSRVLQYLDFFQTDARDRF
                     EIWLSRLGRYEGMIRERLRAKGLPEDLVYLTVIESGLSNTAVSRTRAVGMWQFMAGTA
                     RNYGLTVDPWMDDRRDPFKATDAAANYLSDLMQRLGSVYLAAAAYDAGAGKIERSVAR
                     LPGEVDSLTDQTFFDIASRKGYLRRETRDYVPKLIAASLIAKQPARYGFTGIQPMAPL
                     VFDEITVPDATGLDVIAKLSDTTVAAILELNPQFIRGITPPGREVTVRVPRGRGQTVA
                     LRYDSLPASERVTFVDHYVTRGQTVSGIAQRYHVTVAMITSANPHVRLSTLHIGQRLI
                     IPMSGRVVPAAAWSVPPEHRSRTRTPLQKGSHRVAKGETVTSIAHHYGVPLAALLDYN
                     DLEAHAKLKIGQVIRIPPKQFR"
     gene            complement(2182..>2415)
                     /locus_tag="VFU75_12030"
     CDS             complement(2182..>2415)
                     /locus_tag="VFU75_12030"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HEU5467614.1"
                     /translation="YEPGLSVEQLYEPATNLRLGFRYLHDLMARYGTLNHALVAYNSG
                     PGKLQDLLDAGHEPKSAYAHRVAGGYKAPKNGK"
BASE COUNT          389 a          809 c          838 g          379 t
ORIGIN      
        1 aggccccgca ggcggccagt tcgcggcacc agatcgaagt gcggttcgac tgatgtccgc
       61 cgatcagggt cccaacgggg gagctccgcc cgcgacgacg gagcgcctgc ggttccagga
      121 gttcacgttc cagcggctcc ccaacgggcg ttgccgggcg aaagtacagc tgggacggtc
      181 gggggcgacc ctgttctcag ggcaggccga cggcgtgacc tccccggccg gtgagctccg
      241 gtgctccgcg gaagccgcgg tgaacgcgct ggatgacgcc gtgcaggcgg agatcgagtt
      301 cgagctgctg ggcgtgaagg cggtgcgggc gttcgacgcg acggtggtga tcgtgtccct
      361 ctcggcacgt gaggagtcga aggcgacacg gctcgtgggg acgtacctca cggagaccga
      421 cccgccgcgt ggcgcggcgc ttgcagtgct gaatgcaacg aaccggattc taggcaacca
      481 cttttcgacg agatgacggt ggggcgagcg agcagggtct tcgggtggat ggcggtctcc
      541 gcgctgatgg ctgggtgcgg gggcagagcc acgcccgtcg ccaccgcgcc gacgccggcg
      601 tccgcgccgg cctcggtagc gtccgcggcg ccgaccgaca ccgctaagac gaagctcacg
      661 ggcgccgact ccgtcgctcg cgcccaggcg gccgtggacc gcgacgaggc ggccgatcag
      721 cgggtgctcg acaccttgca gcagggcctg cctgattcta ccagccgcat tgcggttgcg
      781 acgagcagca ttccgcgaga agaagtccgg catgaggccg agcggctctt cggtccggac
      841 aagcccaccg cgccgagcgc cggtccgaag ggtggggacg cgaccttcga catcgacgtc
      901 agcacgtttt ccgggaacag ccgggtcttg cagtacctcg acttcttcca gaccgacgcg
      961 cgtgaccgct tcgagatctg gctgtcgcgc ctgggccgtt atgaggggat gatccgcgag
     1021 cggcttcgcg cgaagggtct gcctgaggac ctggtctacc tgacggtgat cgagagcggg
     1081 ctgtcgaaca cggccgtcag ccggacgcgg gcggtcggga tgtggcagtt catggccggc
     1141 acggcgcgca actacggtct gacggtcgac ccgtggatgg atgaccggcg cgatcccttc
     1201 aaggcgacgg acgccgccgc gaactacctc tccgatctga tgcagcggct ggggagcgtg
     1261 tatctcgcgg ccgcggcgta cgacgccggg gcggggaaga tcgagcgttc cgtcgcgcgg
     1321 ctgcccgggg aagtggactc gctcaccgat cagacgttct tcgacatcgc gagccggaag
     1381 ggctatctcc ggcgcgagac ccgcgactac gtgccgaagc tgatcgcggc gtcgctgatc
     1441 gccaagcagc cggcccggta cggcttcacc ggcatccagc cgatggcgcc gctcgtcttc
     1501 gacgagatca cggtgcctga cgcgacgggc ttggacgtga tcgcgaagct ctccgacacc
     1561 accgtcgccg cgatcctcga gctcaatccg caattcatcc gcgggatcac gccgccgggg
     1621 cgcgaagtga ccgtgcgggt gccgcgcggc cggggccaga ccgtggcact ccggtacgac
     1681 tccctgccgg ccagcgagcg tgtcaccttc gtggaccatt acgtcacgcg gggccagacg
     1741 gtctccggaa tcgcccagcg gtatcacgtc accgtggcga tgatcacctc cgccaacccg
     1801 cacgtgcggc tgtcgaccct ccatatcggc cagcgcctca tcatcccgat gagcggccgc
     1861 gtcgtcccgg ccgcggcgtg gagcgtgccg cccgaacacc ggtcccgcac ccggacgccg
     1921 ttgcagaagg gctcgcatcg tgtggccaag ggtgagacgg tgacgtccat cgcccatcac
     1981 tacggcgtgc ccctcgcggc actcctcgat tacaacgacc tcgaagcgca cgcgaagctc
     2041 aagatcggcc aagtcatccg gattccgccg aagcaattca gatgatagag gtcaggggtc
     2101 aggggtcagg gacacaaaga aggggactcc acacggagcc cccttcttgt ttccctgatc
     2161 cctgatccct gaccccttta gttacttgcc gttcttcggc gccttgtatc cccccgccac
     2221 gcggtgggcg tacgccgact tcggctcgtg accggcgtcc agcaggtcct ggagcttgcc
     2281 ggggccgctg ttgtaggcga ccagggcgtg gttcaacgta ccgtaccggg ccatcagatc
     2341 gtgcagatac cggaagccga ggcgcaggtt ggtcgccggc tcgtacagct gctcgacgga
     2401 caacccgggc tcgta
//
LOCUS       DASUBP010000317        31776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_613192, whole genome shotgun sequence.
ACCESSION   DASUBP010000317 DASUBP010000000
VERSION     DASUBP010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 31776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31776
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_613192"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..740)
                     /locus_tag="VFU75_12035"
     CDS             complement(<1..740)
                     /locus_tag="VFU75_12035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467615.1"
                     /translation="MRAQLERDFERFGRGLPRDFPAHVRERYHIDLSARYLGFPLPHP
                     IGKGSGQLSLHESQLETDHQAGLAFVVLKTVIAQDASGAQTMKAWATHETRMEVERRR
                     FGDREGWTVTWKGRGWDRSFDDYLALVRVARDLTRSGEMVAVPSVKYHLPVLGEAFRD
                     EEYRFTTGALARQWGAGPLPLEKDFSPTLAGDSRSDEREAILRWLAEVPARIRAAGDV
                     RLGVKLMNARFDDDFQRQMVEGTKGADAL"
     gene            complement(718..1596)
                     /locus_tag="VFU75_12040"
     CDS             complement(718..1596)
                     /locus_tag="VFU75_12040"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF012904.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEU5467616.1"
                     /translation="MLTADRLDWGLIPAVPTPFRGREIADDALRGYAKWMAGQPIAGV
                     AVWAHTGRGLRLNPAQRRRVLEVWREALPGKVIVAGANSVEMAREAKALGADAVLAFP
                     TADEPIGFHAALARELPVIAFYLYEAAGGVAYDNRTLHGLLQMPGVIGIKVATLDSVM
                     TFQRIAELARPYRDKVLITGEDRFLGYSFTMGARAALIGMGAALTDLQAELVRAFRED
                     NFGLFVRLSGILDRFSLATFKDPMEGYIRRMLWAAASQGVIPEDVCDDPWGPVLDPAE
                     RETVRQAVRDARSARA"
     gene            complement(1596..2369)
                     /locus_tag="VFU75_12045"
     CDS             complement(1596..2369)
                     /locus_tag="VFU75_12045"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5467617.1"
                     /translation="MRIDVNAFLGAYPWRRLPGTTPAHLIQAMDRVEITEAWVSHLPG
                     LWWKDPTEGNDWLYETAAVHPRLKPVPTVHPSLPHWEEVLAEAATRGAPAVRCDPQFL
                     GLAPAGHEMRVLAATCGSAGVPLLMSVRLEDLRQRHPLDTAGDLPASAVRTLIRADAD
                     LKLIVTHADRSFIEEVHFGSTPAEAERLWWDLSWIWGPPEDHLATLLSNLGPSRFLFG
                     TGQPLRIPEHSVAKLDLLDLSAEARGAIEAGNARRLVAG"
     gene            complement(2369..3193)
                     /locus_tag="VFU75_12050"
     CDS             complement(2369..3193)
                     /locus_tag="VFU75_12050"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEU5467618.1"
                     /translation="MPDSEMLIDVHAHFHPDRSPRNDWREYNASRLAAGDKMGVTIHV
                     ASILGTFGRTSPTYFPSPTDVTYGNDFLLSLEKAHPNRIRGYVCVNPNYTEHALTEID
                     RGVKAGMVGLKLAASRRADDPLLDPVCDAAARHHLPVLHHIWQHRRHEWPQQEASDAL
                     ELCALALRHPTVSFLLAHIGGGGDWLHSLAAVRDVSNVYVDLSGSGVDGGMLEACVDA
                     VGVSRLLWGADMTLCSGWAKLRYLERLLTVEEVEGVKWKNAASIFPKGAFGPALAN"
     gene            complement(3197..4387)
                     /locus_tag="VFU75_12055"
     CDS             complement(3197..4387)
                     /locus_tag="VFU75_12055"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEU5467619.1"
                     /translation="MTESRRIPGITSTGSKRPDALFGPDALDGLAPGVPRLTSRSSGC
                     SVWDEAGREYVDYVMALGAVALGYGHPVVNEAARKAVDAGVVGPQPPALEEELAEALC
                     ARLPGVERVRFLKTGAEAVAAAVRLARVSTGRDLVLTCGYHGWLDWSQGSVEGVPSLL
                     RELRRELPFNDVARTRAAIREAGRSLAAVVVEPLVVQEPSREWLDALRVETRSAGAVL
                     VFDEIKTAFRLAIGGAAERWGVHPDLMVVGKALANGFPLAAVAGQAGVMEAAARTWIS
                     STLATESVSLAAALATLRVFTTESVCAHLHTVGTRLLHGLHSLAAKHKDVVTGVAGVA
                     EMSFLHYVDDRASARVARLCAARGLLFKRTAYNFVSYAHDEKVIDRTLATLDEVLGEV
                     AREP"
     gene            complement(4341..5096)
                     /locus_tag="VFU75_12060"
     CDS             complement(4341..5096)
                     /locus_tag="VFU75_12060"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmJ/YjtD family RNA methyltransferase"
                     /protein_id="HEU5467620.1"
                     /translation="MSLLVVLHEPQDLVNIAHTVRVMKNFGLKDLRIVSPREYDTHRI
                     GGIAHQTADVLARVKLCETLDEALGDCVHVAGFTARGRTAKRNLQRPREAAAELVAVA
                     ETGPTALLFGREDKGLPNEALDRCHRIVTVPTEPTYGSLNLGHAVAIALYEVALAGGA
                     GERAFKVPRREAGPADSAELERLFGDVERALDAIDFFKTRNPEGVMRTVREVVHRVPL
                     DEREAKLLRAMAIEVVKYRDRIAANSGNHVDRE"
     gene            complement(5093..7801)
                     /locus_tag="VFU75_12065"
     CDS             complement(5093..7801)
                     /locus_tag="VFU75_12065"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HEU5467621.1"
                     /translation="MVTIEEIQKRRHELSSSATLRSLYARLEARTTRLLATPPAIPVM
                     KGILSADGGFCSADGSVLTFDPASPTSHSCPQCAAVYSGEHHDQWWARHQHLWLAERA
                     AEWATVAALGEKPEAARGAAALLTEYAQRYLTYPNRDNVLGPSRLFSSTYAESMWVLN
                     YLSAAWLLRECGALDEATAAAVHQVADEAANLIGEFDEGFSNRQTWNDAALTAIAVWF
                     EDEDLAKRAIEGDTGLLAHLRGYRTDGLWYEGENYHLFAMRGLVTGLGWARHAGVELE
                     GDEPLRSRIEDILLAPSFSALPDLTFPARKDSRFGVSLAQPMYLDTWEAAFGRMESEA
                     GRRWLTALYQAAPQPQQLFESYLHDAPYLNTPVTPARDGLSWGALLEMNPDPAPAEPW
                     QGRSVLLEDQGLAVFREGSRYVSLECGPTGGGHGHPDRLHLTVHQDGVHWLPDFGTGS
                     YLRPDLPWYRSTLAHNAPRLDGESQPGWDASCEMFDVQDEWAWARGRADVFTRTVVVG
                     PKYILDVLELDAAGNHRTELPWHLRGDVTVTTPGSWDEKAFDEGVVTKAEHFAPGVRD
                     PIGIEARSGSSSLALRMATDGALFRLTAPGAPGTQRPSSFFLVRRDGDGARFVTVISR
                     EPVTALKLSGDVIEIVQHGGADTHRLHLDGWNVDTPKGRVALRGARDRTERPKPFLNL
                     EPPPRAEGAALRLDEPPALDGTLTGFDVSEPLRLDLEDQYRRSEEPYGGPEDLRARAY
                     VGWAEGTVYVAVEVDKPDVTFRPAQAPPLLLDNEPDDIHSDGIQVYVQMPEDPAVHGY
                     LVVPNAGGALRVHGAGETKGSASEVRGAWSETDTGYRITLALSCADFARAHVGAHVGF
                     DLLVNEMLPGRVRRAGQLVWSGGGGWVWLRGDRQDPGSFGILELVG"
     gene            complement(7795..8808)
                     /locus_tag="VFU75_12070"
     CDS             complement(7795..8808)
                     /locus_tag="VFU75_12070"
                     /inference="COORDINATES: protein motif:HMM:NF020133.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467622.1"
                     /translation="MRRRELPALVRASAPTRLDFAGAWTDVPPFSAREGGAVVNGAIE
                     LRAEAVLEIGGPLIRLEAEDTGTVVECVNAGGLIPDGRLELHKAALRMFPVQGNLTLR
                     TRSAAPPGSGLGGSGALDVALVACLARARGEHWDVRDLAEHAWHLEAVEARFPGGRQD
                     QQAAAYGGFHRFTFRDPDVGVEPITLAPDFADALARHLVLCYTGETRVSSATISRVAG
                     AYEKGDPKVTGALRALKDLAGEMADALRTGDLSRVGRILTWNWERQLDLDEGMRTPAM
                     ARLEQAMTAAGVLGGKAAGAGAGGSMFFVAGDDVDGAVAAARGAGATILPVKWALEGG
                     RAW"
     gene            complement(8805..10262)
                     /locus_tag="VFU75_12075"
     CDS             complement(8805..10262)
                     /locus_tag="VFU75_12075"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEU5467623.1"
                     /translation="MHRPGWWLLVVAGLTAACAGDRAARRGSTVLFAYGADLETMNSL
                     VTRSPLPRQVERYGLLTTLARYDSALQPQPYLARAWTWSADRRTLTFALHTEVRWDDG
                     VQTTAKDVAWTLNAARDSVTGYPRYADLTAIQSVTALDDSTVVIRWSRPPEGFPDVFT
                     DLGILPAHLLSSVPHAQMRSAPWNTHPIGNGPFRFVSFEHNRRWIFESNPDFPAALGG
                     PPHLSRFIVVTVDEPTAKLAALVGGELDFAGVTAIHVPFVRKDPRLQVLEYPLILPYG
                     LVLNVRRPPFDDPKVRLALALAIDRRAIIDGVLFGFGHVADGPVPPELPGYVPVPAIP
                     FDPDSARRLLGGHCPSFDIVTVGSGEAAVEQLIQANLAAVGCQVGIRQYELATFNGMI
                     YRAHDFSAAVMSIPGDPGLSYVHTIGDITGLPLPDDPREAQRVLGEVHPVIWLYHARG
                     VNGMNRRVHGVRMDARGELPTLASWTTTPTATTRP"
     gene            10257..11276
                     /locus_tag="VFU75_12080"
     CDS             10257..11276
                     /locus_tag="VFU75_12080"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5467624.1"
                     /translation="MHARILAALEPGREIVAVLRHLLSRLAVGAAVVAGVVALTFLLL
                     HAAPGNPVDYLLGPTATPSQISAARHSLGLDQPLPKQFVSWLSRFGTGDWGTSIATGR
                     PVREMIATAWPETAWLVGLSLLLSCGLGVVVGALQAARPGTRTDAALSIGTVTLYAVP
                     SYWLGLMLIMLGTYQWKAFPAFGAAGYDADFLGAWARFLDRARHLALPLLTLTLIGIG
                     GMARFARGAMVDVRREPFVTAARAKGLPPRGVTRHILRNALIPVVTLLGLSLPALFSG
                     AVFVEAVFAWPGVGRVLVEAVQARDYPVVMAATAVSAALVVIGNLLADLWVNALDPRL
                     QRQAR"
     gene            11312..12112
                     /locus_tag="VFU75_12085"
     CDS             11312..12112
                     /locus_tag="VFU75_12085"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5467625.1"
                     /translation="MTVLLVAVAVALAAPLVTNGHPDLQADVIQTRFLPPLATDLHGA
                     WHVLGTDRFGRDVWTRLAYGARISLGVGVLAVAISVALGCLVGGVAGASQGWLSRGLM
                     AGTDFALALPRVVLLLLLAALWRPSAGLVVIVLGVTGWMGIARLVYGEVRSLSTRGFA
                     ENARALGATRRRVLWRHLLPNALTPVIVAAALGVGNAILLEAGLSYLGLGVQPPTASW
                     GSMIASGRDTLVNAPWVAAAPGIALVLVVVACTLLGDALRDRLAPHTT"
     gene            complement(12109..12744)
                     /locus_tag="VFU75_12090"
     CDS             complement(12109..12744)
                     /locus_tag="VFU75_12090"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HEU5467626.1"
                     /translation="MEERSIRVSRTARYATLGEPGPKLSELWMVCHGYGQLARRFAED
                     FEGLADGTRMVVAPEGLSRFYIDRTQQRVMGASWMTREDRLHEIEDYLGYLDALYAEV
                     RGRIGRDLPVTALGFSQGVATATRWAARTRAPVKRLVVWGGTVPPEVDLSVFARTRVT
                     VVCGDQDELITAKVRETEAGLWRKVGIDPEVKIFPGHHEVLPEVLAELARA"
     gene            complement(12751..15360)
                     /locus_tag="VFU75_12095"
     CDS             complement(12751..15360)
                     /locus_tag="VFU75_12095"
                     /inference="COORDINATES: protein motif:HMM:NF013256.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase-related protein"
                     /protein_id="HEU5467627.1"
                     /translation="MTLTFHLIPHTHWDREWYLPRAAFTARLVPAVDDLLALLESDPA
                     YRSFLLDGQTVLVEDYLAVRPDREASLRALAQSGRLQLGPWYVLADELIPSGESLIRN
                     LLYGAIDTARWGGRLDVAYSPDAFGHPAILPVLAREFGLKAAALWRGLGGEPGQTRDA
                     YRWRGPDGRDLLLWHFPPDGYEAGAALPTDRERLRQAWVPLRAELTRRAATNHIAVPV
                     GADHHAPYPDLPTLRERIAELEPQNAVRISRLDEFLKELATEAEHAPEVSGELRWSYR
                     YTWTLQGVHSTRAPLKRRHSRAELQLERIVEPLAALARRAGGRDRRALLDRAWRTLIR
                     GTFHDTLCGCCADPVARAQGTRLDAVEATSRELADGAADDLSGHDPDLSRDHPHPDSA
                     LWLWNPVARTRSGVVIADTTWFRRDILVGPPGDRKPRHGHAPGEFALMNQDGEAIPIQ
                     VLSRRKALERRDAARHYPDQDEVDHIRIAFHAPALEGLRATVLRSRVSAPLRRDTGAG
                     VHGRTLSNAFLDVTVEPTGAVTLLDRRTNERFEGLLRLEEERDLGDLYTPFPQGRPVR
                     AGGPIRVRRLAGGPLVAALEVAYDLPGAAARLVLQLFDESPVLRCLLDIDNRVADHRL
                     RLCCPVGVTGPALAGAAFGAVRRGPVSVHAEDYPRETPVTTAPGHHFVAAAQGNRGLA
                     LLAPGFFEYEWTPEGELRFTILRGAGELSKDDLPTRPGHAGWPTSTPDGQCLGRDRIE
                     LALVSVGARDLDAGETLPELWEDVFLPTRARWLRNANLGGPKAFGATLDGRGLVLSAV
                     KPAQTGSGMVLRCFNARGERVVGAWRLPDTIRTAHRVRADEKSAEPLVVEGRGHTVRF
                     IAEPHEIVTLLIT"
     gene            15425..17761
                     /locus_tag="VFU75_12100"
     CDS             15425..17761
                     /locus_tag="VFU75_12100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional YncE family protein/alkaline
                     phosphatase family protein"
                     /protein_id="HEU5467628.1"
                     /translation="MLRKIALSVSGLLVVGVAAHRIVPGALGNGTTVLPSGWRISPAG
                     KQVPVGTLPLNILRLANGQVVVTLNGHSANGLWRIDAGTGKIVDSLPFKAAWLGLAHR
                     GTATADTVWASGGSTNRVYRVIYGSGVPAVDTVVLAPPPAAIFPAGIAILPGRDLVAV
                     VGNLSDSVYILDTRTLAVTAKFAVGHHPYTAVADSAAVYVSNWGDSSVERLPITAAGV
                     VDGAKLAVGPHPSSMVLRGHQLFATLAGANEVARYDLDSNRVTDRLAVALMRGAPTGS
                     DPNALALTPDGRRLFVALAGNNAVAVVNVAAATPTVEGLLPVGWYPTGVEVSRAGDTL
                     YVANGKGNGSGPNPSGPYIADLVRGSVSVIPLPDASGLASATAKVLSLSPYSNPGLRA
                     APPDLPKIRHVVYVIRENRTYDQVLGDAKRGNGDAKLAIFNDSVTPNAHAITSHFVLF
                     DNFYVDGEVSADGHEWTDRAFAGEYNEKTWPAVYSSRRDWDLDDGSDLANPHGAYLWD
                     AARRAGLWTVNFGEAVHTDSSGRHVSSMPGLQGITVPEFPGFELDIRDTTRARLFADS
                     VNAWDQRDTFPALVILYLPRDHTEGKRATHPTPRAMVADNDLALGQVVERLSRSKAWK
                     SMAFFALEDDAQDGPDHVDAHRSVLIMASPYARRGVVDSTMYSTSSVVRTIGIFLGLS
                     PLSQYDAGATPLSHAFVSRADLTPFHAVAPRWPFDELNGKQSMSRLTDQDLAGPDLAD
                     ETILNQEIWQSVRGREAVPAPHRTYLPGRATAGDDDDR"
     gene            complement(17769..19091)
                     /locus_tag="VFU75_12105"
     CDS             complement(17769..19091)
                     /locus_tag="VFU75_12105"
                     /inference="COORDINATES: protein motif:HMM:NF012562.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467629.1"
                     /translation="MATPDPDRWGWREAPRKSLHEWPRLAEFAARARADGLTHTVVCG
                     MGGSSLAPWVLASSFGAKNLTVLDSTDPASVRAAEHASDPAQTLYLIASKSGTTVESM
                     AACHHFARLAPSSHFAAITDPGTPLEHKARGERWREVFAHPADVGGRYAALTVVGMLP
                     LALIGGPGQAVLESAMAVDEAKARELGASIGAAARHGQDKLALRGGGPISSVAAWIEQ
                     LVAESTGKQGRGVVPVLGAGWHTKDVFAADIGAAEPVSLGATFLTWEYAAVAICEALD
                     VNPFDQPDVEAAKVLARAELARPGGAAEASPSTLSPKALASSARPGDYVAILAYLPQT
                     PANDARLGALAGLWHGATGCVVTTGYGPRYLHSTGQLHKGGPDTGLFLVVTADPDQDI
                     AIPEMQTTFAKLERAQAVGDIKALLAKARRVSHVHLRDPDDWSVLSPA"
     gene            19151..21328
                     /locus_tag="VFU75_12110"
     CDS             19151..21328
                     /locus_tag="VFU75_12110"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5467630.1"
                     /translation="MTPPAVRADLDRRGLWIAIGALASYIVMSAAYLLNPASQKSLAP
                     VAWTWASLYATIAALVARRQAGESVERRALGWIAAGCAIWLGGQLGDDWSYLKDAPVS
                     LAKGIAAVGYVGIYPCLVVAVAILLRPQLRRVSGVAVILDTLLLTFTSGILTFELLSE
                     QGPVTDFRRAFLGIVGGLGAFVLLWSILVGVLNRTRTLQEHGSRAFGALVVFALGSVA
                     YAAAQILTGEIGAQRLPLWIVTLGWTALFYILAAGIAWQTPLWRARAFTDAKTGVLSH
                     LVVLIAGLGGVFGVAVIGILRPGVDVPAAILVGAGGIIIAARLVLALRTDQEYARRLE
                     RDVERQTRSLSESYAEQRRIEAQLQQSDKLAALGQLVSGVAHEINNPAAIISGFAQTM
                     LLDELPAEQREMAEMVRDEAMRIGRITSNLLAFARSGGSERDLIDVNDVVRRTYALRS
                     YHLNTLNIAVAVDLSPETPVVWGNGPQLQQMLLNLLINAEQALAAQHGNRSIAVRTVA
                     APDTVRIEVADTGPGVPSEIQAQIFDPFFTTKPVGVGTGLGLSICYGIVQEHGGRIVV
                     DSRPGQGATFIVQLPRDTRPLPKATPESASQTAVDPAGLRVLLVDDEPALREAARRFL
                     ERSGIVTDVAADGQAALEALGARAYDVIISDVRMPGMDGRDFIARLRKDRPELLDRLI
                     LSTGDTFAEDTAGLVAETGVPTLVKPFDFDTLERIVRKTAGAR"
     gene            21567..22076
                     /locus_tag="VFU75_12115"
     CDS             21567..22076
                     /locus_tag="VFU75_12115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467631.1"
                     /translation="MTRPALVVALAVSTLLPRISSAQACSANNCRATNEVVATVGALM
                     GLSLDRPAAVTVNPSARDIATGAQELSGPTARVKSNTDWRLEVSAASTEWAAGQGARS
                     GKPATDLGWRVNANGGYTPLSTDPAQAATGSHTSNTEVAFQYQTSYHSQQDRPGTYSM
                     VVRYTLTTR"
     gene            complement(22200..22733)
                     /locus_tag="VFU75_12120"
     CDS             complement(22200..22733)
                     /locus_tag="VFU75_12120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467632.1"
                     /translation="MKRGLWIAVVLLGAGRVLAAQTCTASLSGGAGTCSVNVTATMPV
                     NAVVQLSLSTTTTALTAPGSADYDAGFVANTGPSATVQCNVTCRLQISAATATWSAVA
                     TTSVTPRSKPCADLTWSTSAGGTFVGLTTTPATVQTIPATGGSAPVSIFYQTKYAWGL
                     DTPATYSLTVVFTLAAP"
     gene            complement(22730..23152)
                     /locus_tag="VFU75_12125"
     CDS             complement(22730..23152)
                     /locus_tag="VFU75_12125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467633.1"
                     /translation="MNPALGLCGILLAACVTSTDTGSSTLVVTGTWDYAASQSTPTTA
                     NITGTLIVSSQSNGIFQGSASGSETEGSTTTPVSGPIAGQTVNDTTVDFDIFFNSDPN
                     GRRHVAAVVRDTMRGSWVETGGAGTFAGSFTAVRRSGP"
     gene            complement(23149..26121)
                     /locus_tag="VFU75_12130"
     CDS             complement(23149..26121)
                     /locus_tag="VFU75_12130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467634.1"
                     /translation="MKRSVVIGALSFTLVAAAAGATVTLRNVIRHGARRSVSAAAPSA
                     APVPAVIRPDTLIQTVNPAAPATRTSLRRATLTPAAAPAQRVAPAHEAPAALTPIAPA
                     APVAHLDLEPVEAAAAPRSVQVVAVPVPAALLSGGDVQYQVVPEGDAHLVGALNGTVA
                     VTSGEANRPVVVAANLSKSAPAGVQTVARVQFSQSGAVRGEVLVRLNVLTIHSALIRL
                     GQTVLGGHAGDRIPLRYFVTNTGNATDTLAVRLLAPGGWRVVGAPTTIVIPAGATATE
                     EVTLWVPSDAGIGSVRLGVIVSAGGRDLARADAGLEVVAGAALHGGPSLQVNSGVASV
                     LTNRTSAPVFEVDATGPLANGLTMSGRFIQATNTATADPIALARVGYYLGGSYLSVGA
                     SHWRATAGATGHTFSDITGLNAYGRGASFSFDDTRYTADVLGASPSVGTQSSSGHLYG
                     ARIGMHVDGGWVGATATDLQDNVFAPRQLQAIGVGGMSPAFSGFTLSGELAHRSYATG
                     SGMGWSAEVDQRSADNQFQLRALSAPGGAAAYARAKSEFDAQAAHTIGNLQLNGSAFL
                     STDQNQGFGSLRSVGWSVSPRYEVDSHLSLTLDAHGSAYSVNGAVGSFGSNETAVRAG
                     AAMHVGPFYASGGLTAGQVSRTTNLPNAPSIVDAGGRYGIEGTMGASTDRGLFEAGVS
                     YDQSAARVGYLPHEALLSLRADNVPVWGNSRVHAEVQQYLWFGALPSATLVRLGVVVP
                     LPQDLHLTVDVQHNPLLTGPTGGNRWVPVVKLEHTLSVATGSSHALVRGVVYEDGNGN
                     GVRDRGEAGVPGVVVRRGDETTVTDKNGEFTLIQETAERLRVDETSLPFGDVANPASE
                     LQSPDQRRVEIGIWATASVQVRLVPTADETGRVPRVDFRPGLVNAVDSAGNVWSARTD
                     STGTARFDALPPGTYHLDFQLQDVREPVHTAGPAPTFVVKPGATVPAIRVPVYPRPVR
                     LFDPTNQRGRGGATKGHG"
     gene            complement(26118..26594)
                     /locus_tag="VFU75_12135"
     CDS             complement(26118..26594)
                     /locus_tag="VFU75_12135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467635.1"
                     /translation="MRRAILALAVLLPARVFAQGSVVLTVSGGPVAIPAPAVADYNAG
                     FVLDPSALSYNVNIAGGPPSTLHTTTVSVRSASATFGPTALSDLQWSVSGSGVWQSLT
                     TSDVTVESHQISRNAGNNWSNSLLFRCLIGWTNTPPSPPAVAYTTTLVITLTVTTP"
     gene            complement(26594..27448)
                     /locus_tag="VFU75_12140"
     CDS             complement(26594..27448)
                     /locus_tag="VFU75_12140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467636.1"
                     /translation="MTLVFSLTTRRVRWSVLAALSVWGLAVAAPATAQVSVDQAEIVL
                     HPQPSSPAVAGFNVSNETPTTVEAKVYLKDWDRDEDGNNRFLELGTVSQSCAKYLRVF
                     PLSLRLPPHTSQGVRLGLQGADSLPATCWSIVFVEAAPPQAGAGRQVQYVLRLGVKVY
                     IEPPGLSVDGDVEGMGIDTIHAVRVPAVKGAKPVPPAADTVVTSVQFRNTGGLPAIVK
                     GEIEYRRLDNSVVHRDSVPTFYVLPGAARRARVPLPALPKGHYVVLALLDFGGSEVAA
                     GQVPLDVP"
     gene            complement(27445..27957)
                     /locus_tag="VFU75_12145"
     CDS             complement(27445..27957)
                     /locus_tag="VFU75_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467637.1"
                     /translation="MRNRLTVALAAVLGLAFSATASAQTCTTAGCSVSTTASVTIPTL
                     QKLTLSATTTALGVPTIDNVDSAANPVQTAGPSVTGKANVPFSVTIASGAANFTAPAG
                     VTKSAGDLSWSLTAGGTYAALSTTGANVISQTTTGGSQSVNIFYHAAWHSTDAPGAYS
                     LAVVYTLTEP"
     gene            complement(28252..28488)
                     /locus_tag="VFU75_12150"
     CDS             complement(28252..28488)
                     /locus_tag="VFU75_12150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467638.1"
                     /translation="MPPLNRRKAADRRDLQDRRAGVDRRFQGDRRLVVVAVVVERRAG
                     ADRRAGHMERRLAVRRSSSERRTIMERRRAGPRA"
     gene            28604..29659
                     /locus_tag="VFU75_12155"
     CDS             28604..29659
                     /locus_tag="VFU75_12155"
                     /inference="COORDINATES: protein motif:HMM:NF016214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEU5467639.1"
                     /translation="MDNIAHSLVGAAIGRAIGDSRIPAPALLGALAANAPDYNEFLPG
                     MPPRSNFNHRGITHSLLGAVIQAAVFVIVTGLVMLPFCRRENRPVPWKTIVGLYGAAL
                     ASHLLMDWQGSYGLRPFLPWSNQRYYGDWVAIVDPFFWLVPAVFLAWGHVRRWTGALG
                     WAVLMLALVAIVLFASLFFDRAVATWVDMASIAIPVIGIVGWHRHWFAPGAKRTAAAT
                     SLVLLGLYTGAQAVTAMVLKHQLGIDARQRFGPQATYALLTRPGFPFTWTPVMGGADS
                     VGGPGWSLPRHLDSPVVRRVLTTPRAKAAMAFDRFVTADVDSTSTPVMVYLRDTHGPS
                     VGRRGFGIIALETSSTP"
     gene            29674..31434
                     /locus_tag="VFU75_12160"
     CDS             29674..31434
                     /locus_tag="VFU75_12160"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HEU5467640.1"
                     /translation="MPISRPSNPTTQALTEATETPPVDALQALASKLDPMFRLDHLVT
                     LSRDRALYQAWDRVLKRFVALRVHLIPGTPSRTWFMRETETLAALDHPAIRHAYAAAE
                     IGDVAYRTANWIDGESLSEAIRRGPRPIPTVMSLVRDLLGALEYAHAHGVIMRRILPT
                     TLMLEISGRGIVTDLRYANWCLPNVPPDEQGGGAPYTAPEIRSGGAGEPASDLYAVAG
                     LVYLALTGQDPAADPTAIVPLRTVRPAIPAAVERVVLRALKPEPSARYYTATEMLEDF
                     VADAGSFHEPAAAPQLMDSGFERRLRRALGDDYELLGELGSGGFGRVYRARDLGLERE
                     VAIKVLHPSLTADPVVVERFRREAQLAARLRHPNIVSIYDIQGRAGLQWYTMEYVPGQ
                     SLAQLVQGKGPLSVERTVRVMNEALSALEHAHGLGLVHRDLKPENMLLEPDGRLRITD
                     FGLALALRGDRYGGATSRSGTPQFAAPEQLLGERVDPRADLYSLGATAYFALLGRPPF
                     EGTTPEAILAKQTVDALPLLAAERSDVPRELEVVLRRAVTNDPSKRFQQAAAFREAVL
                     AAAGGFRRRLVTVLRRLAGA"
     gene            complement(31516..>31776)
                     /locus_tag="VFU75_12165"
     CDS             complement(31516..>31776)
                     /locus_tag="VFU75_12165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5467641.1"
                     /translation="IEGVSGLMNLAMLPMWILSGTFFSYSRFPDVMLPFVKALPLTAL
                     NDSLRAVMIDGTSIFHLGPPLAIVASWGLVCFGVALKIFRWR"
BASE COUNT         5118 a        11093 c        10634 g         4931 t
ORIGIN      
        1 agcgcatccg cgcccttcgt cccctccacc atctgccgct gaaaatcgtc gtcgaatcgt
       61 gcattcatca gcttcacgcc cagccgcacg tcgccagcgg cacgaatccg ggccgggacc
      121 tccgcgagcc accgcagaat cgcctcccgt tcatcggacc gagagtcgcc ggccagggtc
      181 ggggaaaaat ccttctcgag aggcaatggc ccggcgcccc actggcgcgc gagtgctccg
      241 gtggtgaacc ggtactcctc gtctcgaaat gcctcgccga gcaccgggag gtgatatttc
      301 acagagggaa cggccaccat ctcaccgctt cgcgtgaggt cgcgcgccac ccgcaccagc
      361 gcgagatagt cgtcgaagct ccggtcccac cctcgcccct tccaggtgac cgtccacccc
      421 tcgcgatcgc cgaaacgtcg ccgctcgacc tccatcctgg tctcgtgggt cgcccaggcc
      481 ttcatggtct gcgcgccgct cgcgtcctgc gcgatgaccg tcttcagtac cacgaaggcg
      541 aggccggcct ggtgatcggt ctcgagctgg gattcgtgaa gggataactg gcctgatccc
      601 ttgccgatcg gatgcggcaa cgggaaaccc aggtagcggg cactcaggtc gatgtggtag
      661 cgctcccgca cgtgcgcggg aaaatcgcga ggcaagccgc ggccgaaccg ctcgaaatca
      721 cgctcgagct gagcgcgcat cccgtaccgc ttggcgcacg gtctcccgct ccgccgggtc
      781 gagcaccgga ccccacggat cgtcgcacac gtcttcggga atgacacctt gcgatgcggc
      841 cgcccacagc atccggcgga tatacccctc catcggatcc ttgaacgtcg cgagggaaaa
      901 gcggtcgaga atcccggaga gccggacgaa gagtccgaag ttgtcctcgc ggaaggcgcg
      961 gaccagctcg gcctggagat ccgtgagtgc cgcgcccatg ccgatcaacg ccgcgcgcgc
     1021 gcccatggta aaggaatagc cgaggaagcg gtcttcgccg gtgatcagga ccttgtcccg
     1081 ataggggcgc gccagctcgg cgatccgctg aaaggtcatg accgaatcga gcgttgccac
     1141 cttgatgccg atgacccccg gcatctgcag aagcccgtgg agggtgcggt tgtcgtacgc
     1201 gacgccgccg gccgcctcat agagatagaa cgcgatgacc ggaagctccc gggccagcgc
     1261 ggcgtggaac ccgatcggct cgtccgccgt cggaaatgcc agcacggcgt ccgcgcccag
     1321 cgccttcgct tcgcgcgcca tctcgaccga gttcgcgccc gcgacgatca ccttgccggg
     1381 aagcgcctcc cgccagacct ccagcacccg gcgacgctga gcggggttga gacgaaggcc
     1441 gcgtcccgtg tgggcccaca cagctactcc ggcgatcggc tgtcccgcca tccacttggc
     1501 gtagccgcgc aaggcgtcat cggcgatctc acggccgcgg aacggcgtgg gcacggccgg
     1561 aatgagcccc cagtccagac ggtcggcggt gagcatcagc cggccaccag ccgccgcgcg
     1621 tttccggctt cgatcgcccc gcgcgcctcg gcggagaggt ccaatagatc cagtttggcc
     1681 acggagtgct ccggaatgcg cagtggctgt ccggttccga agagaaacct ggacgggccg
     1741 agattggaca ggagcgtcgc gaggtggtcc tccgggggtc cccagatcca gctcagatcc
     1801 caccagagcc gctccgcttc cgcgggcgtc gagccgaagt gcacctcttc gatgaacgaa
     1861 cgatccgcgt gcgtcacgat gagtttgaga tccgcgtccg cgcgaatcag tgtccggacc
     1921 gcggacgcgg gcaagtcgcc ggcggtgtcc agcgggtgcc gctgacgcag atcctcgaga
     1981 cgcaccgaca tcaggagcgg caccccggcg gatccgcagg tcgcggccag cacccgcatt
     2041 tcatggccgg caggcgcgag ccccagaaac tgcggatcac accgcactgc cggcgccccg
     2101 cgcgtggccg cctcggccag cacctcttcc caatgcggca acgagggatg aaccgtcggc
     2161 acgggcttga ggcgggggtg aacggcggcg gtctcgtaga gccagtcgtt cccctcggtg
     2221 gggtccttcc accacagccc gggcaaatgg gagacccatg cctccgtgat ctccacccgg
     2281 tccatggcct ggatcagatg cgccggggtc gtgcccggaa gacggcgcca cggatacgcg
     2341 cccaggaatg cgttgacgtc gattcgcatc agttcgccag agcgggcccg aaggcgccct
     2401 tggggaagat gctcgcggca ttcttccact tcacgccttc gacttcctcg acggtgagca
     2461 gacgttccag gtaccgcagc ttcgcccaac cggagcagag cgtcatgtcc gcgccccaga
     2521 gcagccggct cacgcccacc gcatctacgc acgcctcgag catgccgcca tccacgcccg
     2581 aacccgagag atcgacgtag acgttggaga cgtcccgcac ggcggcgagc gaatggagcc
     2641 agtccccgcc cccgccgatg tgcgccagga ggaacgacac cgtcgggtgc cggagcgcca
     2701 acgcgcacag ctcgagggcg tccgaggctt cctgttgcgg ccattcgtgc cggcgatgct
     2761 gccagatatg gtggagaacg gggaggtgat gccgggcggc agcgtcacag acgggatcga
     2821 gcagcgggtc gtccgcacgc cggctcgcgg ccagcttgag cccgaccatg ccggccttca
     2881 caccgcgatc gatctcggtg agcgcgtgct cggtgtagtt cgggttgacg cacacgtagc
     2941 cgcgaatgcg gttggggtgc gccttctcga ggctcagcag gaaatcgttg ccgtaggtga
     3001 cgtcggtggg ggagggaaaa tacgtgggtg acgtccgccc gaaggttccc agaatcgacg
     3061 ccacgtgaat ggtgacgccc atcttgtccc cggcggcgag gcgggacgcg ttgtactccc
     3121 gccagtcgtt gcgcggactc cggtccggat ggaaatgcgc atggacgtcg atcagcatct
     3181 cagaatctgg catctctcac ggctcgcgcg ccacctcacc cagcacctcg tcgagcgtgg
     3241 cgagcgtgcg gtcgatcacc ttctcgtcgt gagcatagga cacgaagttg taggcggtgc
     3301 gcttgaacag gagtccgcgg gccgcgcaga gacgggccac acgcgcggac gcgcggtcgt
     3361 cgacgtagtg cagaaaactc atctcggcga cacccgcgac gcccgtgacg acgtccttgt
     3421 gcttcgcggc gagcgaatgg agcccatgca ggagccgagt ccccacggtg tgtaggtggg
     3481 cgcacaccga ctccgtcgtg aacacgcgaa gggtggccag cgccgccgcc agagaaaccg
     3541 attcggtagc gagtgtggag gagatccacg tccgcgccgc ggcctccatg acaccggcct
     3601 ggccagcgac cgccgcgagc ggaaagccgt tggccagtgc cttccctacc accatgagat
     3661 cgggatgcac accccagcgc tccgcggcgc cgccgatcgc gagccggaac gcggttttga
     3721 tctcgtcgaa cacgaggacc gcgccggcgg acctggtctc gacacggagc gcgtccagcc
     3781 attcgcggga cggctcctgc accaccagcg gttcgacgac caccgccgcc agtgatcgtc
     3841 ccgcctcgcg aatggccgcg cgggtcctgg cgacgtcgtt gaagggcagc tcgcggcgaa
     3901 gttcccgaag caagctggga accccttcca cgctgccctg tgaccagtcg agccatccgt
     3961 ggtagccgca ggtcagcacc agatcccggc cggtcgagac ccgggccaac cgcaccgccg
     4021 ccgcgactgc ttccgcgccg gtcttgagga accgcacgcg ctcgaccccc gggagccgcg
     4081 cgcagagggc ttctgccagc tcctcctcga gcgcgggagg ctggggaccc acgacgccgg
     4141 cgtcgaccgc cttgcgagcg gcctcgttca cgaccgggtg tccgtagccc agcgcgacgg
     4201 cgccgagcgc catcacatag tcgacgtact cccgccctgc ctcgtcccag acggagcatc
     4261 cggacgaccg cgacgtgagc cgcgggacgc ccggagcaag gccgtccagg gcgtcgggcc
     4321 cgaagagcgc gtcgggccgc ttactcccgg tcgacgtgat tcccggaatt cgccgcgatt
     4381 cggtcacggt acttcaccac ctcgatggcc atggcgcgga gcaatttggc ttcgcgctcg
     4441 tccaggggca cccggtgcac cacttcgcgc accgtccgca ttaccccttc ggggttccgc
     4501 gtcttgaaga aatcgatcgc atcgagcgcc cgttccacgt ccccgaacag acgttccagc
     4561 tcggcggagt cggcgggccc ggcttcgcgg cgcgggactt tgaatgcccg ctcgccggcc
     4621 ccgcccgcga gcgccacctc gtagagtgcg atggcgacgg cgtgaccgag gttcagtgat
     4681 ccgtacgtcg gctcggtagg gaccgtgacg atccggtggc agcgatcgag ggcctcattg
     4741 gggaggccct tgtcctcacg tccgaagagc agcgccgtcg ggcccgtctc ggcgacggcc
     4801 acgagctcgg cggccgcctc gcgcggtcgc tggaggttgc gtttggcggt ccggccccgg
     4861 gccgtgaacc ccgcgacatg cacgcagtcg ccgagggcct cgtcgagcgt ttcgcacagc
     4921 ttcacccgcg cgaggacgtc ggcggtctgg tgggcgatcc cgccgatgcg atgcgtgtcg
     4981 tactcccggg gtgagacgat ccggagatcc ttcagcccga agttcttcat cacccggacc
     5041 gtgtgagcga tgttgaccag atcctgaggc tcgtggagca cgaccaggag actcatccga
     5101 cgagctccag gatgccgaag ctccccggat cctgccggtc accgcgcagc cacacccacc
     5161 ccccgccgcc actccacacc agctggccgg cgcgccgcac acgtcccggg agcatctcgt
     5221 tcaccaggag gtcgaacccg acgtgcgccc cgacgtgcgc gcgtgcgaag tccgcgcagg
     5281 agagagccag agtgatccgg tagccggtgt cggtctcgct ccaggcgccg cgcacctccg
     5341 aagccgatcc cttagtctcc ccagctccgt ggacccgcag cgcgccaccc gcgttcggca
     5401 cgacgaggta gccgtggacc gcgggatcct ccggcatctg aacgtagacc tgtatgccgt
     5461 ccgaatgaat gtcgtcgggc tcattgtcga gcaggagcgg cggcgcttgc gcgggccgga
     5521 acgtcacgtc gggcttgtcc acctcgaccg cgacgtacac cgtcccctct gcccagccga
     5581 cgtaggcccg cgcacgcagg tcttccgggc ccccgtaggg ttcctccgac cggcggtact
     5641 gatcctccaa gtccaagcgc agcggctccg agacgtcgaa tcccgtgagc gtgccatcca
     5701 gggccggtgg ttcgtccagc cgcagggccg cgccctcagc ccgggggggc ggctcgagat
     5761 tcaggaaggg cttgggccgt tcagtccggt cgcgagcccc gcgcaatgcc acgcgtccct
     5821 tcggggtgtc gacgttccag ccgtcgaggt gcaaccggtg cgtatcggcg cctccgtgct
     5881 gcacgatttc gatcacgtcc cccgacaact tgagggcagt gaccggctcc ctggaaatca
     5941 cggtgacgaa tcgagcgccg tccccgtctc gcctgacgag gaaaaatgaa gatggtctct
     6001 gagtgccggg ggcacccggc gccgtgagcc tgaacagcgc tccatcggtg gccatccgga
     6061 gggcgaggct gctcgacccg gaacgggctt cgatgccgat cggatcgcga accccgggcg
     6121 cgaagtgctc cgccttcgtg accacgccct cgtcgaacgc tttctcgtcc caggatcccg
     6181 gcgtcgtgac cgtgacgtcc ccgcgcagat gccaggggag ctcagtccgg tgattgccgg
     6241 cagcgtcgag ctcgagcacg tcgaggatgt acttggggcc gaccaccacc gtccgggtga
     6301 acacgtccgc acgcccgcgg gcccaggccc attcgtcctg cacgtcgaac atctcgcatg
     6361 aggcgtccca gccgggctga gactcgccgt cgagacgagg ggcgttgtgt gcgagcgtgg
     6421 agcgatacca gggcaagtcg ggccggaggt aggatccggt gccgaaatcg gggagccaat
     6481 ggaccccgtc ctgatggacg gtgagatgca ggcggtccgg atgtccgtgc cctccccccg
     6541 tcggtccgca ttcgaggctt acgtagcggg agccctcgcg gaaaacggcg agcccctgat
     6601 cctcgaggag gacactccgg ccttgccaag gctcggccgg ggccggatcg gggttcattt
     6661 ccagaagagc gccccacgag agcccgtcgc gcgccggggt gacgggagtg ttcaggtacg
     6721 gcgcatcgtg caaataggac tcgaacagtt gttgtggctg cggagccgcc tggtagagag
     6781 cggtcagcca ccgccgccca gcttcactct ccattcgtcc gaaagcggct tcccaggtgt
     6841 cgagatacat gggttgcgcc agggagacgc cgaaacgcga atccttccgc gcggggaaag
     6901 taaggtccgg aagcgctgag aatgagggag ccagcaggat gtcctcgatg cggctccgga
     6961 gcggctcgtc tccctccagc tcgacgccgg catgcctagc ccatccgagg cccgtgacca
     7021 agccccgcat ggcgaacagg tggtaattct cgccttcgta ccagagccca tcggttcgat
     7081 agccccggag gtgcgcgagc aatccggtgt ccccttcaat cgcccgcttc gcgagatcct
     7141 catcctcgaa ccacacggcg atcgccgtca gcgccgcgtc gttccaggtc tggcggttcg
     7201 agaagccttc gtcgaactcc ccgatgagat tcgcggcctc gtcggccacc tggtggacgg
     7261 ccgcggcggt cgcctcatcg agcgcgccgc actcccggag cagccaggcc gccgacaggt
     7321 agttgagcac ccacatcgat tccgcgtagg tcgatgaaaa aagccggctg ggaccgagga
     7381 cgttgtcgcg attcggatag gtgagatagc gctgcgcgta ctcggtgagc agggcggctg
     7441 ccccccgcgc ggcttccggc ttctcaccga gcgccgccac cgtcgcccac tcggcagcgc
     7501 gttcggcaag ccacaggtgc tggtgcctgg cccaccactg gtcgtggtgc tcgcctgaat
     7561 agacggctgc gcactggggg caggaatgcg acgtggggct cgcggggtcg aacgtcagga
     7621 ccgagccgtc cgccgaacag aatccgccgt cggccgagag aatccccttc atcacgggga
     7681 tcgcgggggg ggtagcgagc agtcgtgtgg ttcgggcctc gagtcgcgcg tacagggacc
     7741 gcaacgtggc cgacgacgac agctcgtggc ggcgcttctg gatctcttcg atggtcacca
     7801 cgcgcggcca ccctccagcg cccacttcac gggcaagatc gtcgcacccg cgccccgcgc
     7861 ggccgcgacc gccccatcca cgtcgtcacc cgccacaaag aacatcgacc cgcccgctcc
     7921 tgcccctgcg gccttccctc ccagcactcc ggccgcggtc attgcctgct cgagccgtgc
     7981 catcgcgggt gtgcgcatgc cctcgtccag gtccaattgc ctctcccaat tccaagtcaa
     8041 gatcctcccg actcgggaca ggtcaccggt ccggagggca tcagccatct cgcccgccag
     8101 gtccttgagc gcccggaggg ctcccgtgac cttcggatcg cccttttcgt aggcgccggc
     8161 cacccgcgaa atcgtggcgg aggacacccg ggtttctccc gtgtagcaga gcaccaggtg
     8221 ccgggcgagc gcgtccgcga aatcgggagc cagagtgatc ggctcgaccc ccacatccgg
     8281 atcgcgaaac gtaaaccggt gaaaaccccc gtaggctgcc gcctgctggt cctgccggcc
     8341 accgggaaaa cgggcctcga cagcctcaag atgccacgcg tgctcggcca ggtcccggac
     8401 gtcccaatgt tcgccccggg cccgggccag gcaggcgacg agcgcgacat cgagcgcgcc
     8461 tgaccccccc aggcccgacc cggggggcgc cgcgctgcgc gtgcgcaagg tcaggttgcc
     8521 ttgcaccgga aacatccgga gcgcggcctt atggagctcg agacgtccgt cgggaatcag
     8581 tccccccgcg ttgacgcact cgacgacggt ccccgtgtcc tccgcctcca accggatgag
     8641 tggcccgccg atctcgagca cggcttccgc acggagctcg atggcaccat tcaccacggc
     8701 cccgccctct cgtgcggaga acggcggaac atcggtccac gcgccggcga aatcgagccg
     8761 ggtcggcgca ctggcgcgca ccagcgccgg gagctcgcga cgcctcatgg cctggtcgtc
     8821 gcggtcggcg tggtcgtcca ggaggcgagc gtcgggagct ctccgcgcgc gtccatgcgc
     8881 acgccatgaa cgcgccgatt cattccgttc acacctcgag cgtgatacaa ccagatcacc
     8941 ggatggactt cgccgagcac gcgctgcgcc tcccgcgggt catcggggag gggcaggccc
     9001 gtgatgtcgc cgatagtgtg gacatacgac aaaccggggt ctccggggat gctcatgacg
     9061 gcggcgctga aatcgtgcgc gcgatagatc atcccgttga aggtcgcgag ctcgtattga
     9121 cggatgccga cctgacatcc caccgcggcc agattcgcct gaatcaattg ctccaccgcc
     9181 gcttctccgc tgccgaccgt gacgatgtcg aatgacgggc aatgcccgcc gagcagccgc
     9241 cgcgccgaat caggatcgaa gggaatggcc ggcaccggga cgtagccggg aagctcgggg
     9301 ggcaccggcc cgtcggcgac gtggccgaat ccgaagagca cgccgtcgat gatcgcacgg
     9361 cggtcgatcg cgagggcgag cgccagccgc accttcgggt cgtcgaaggg agggcgtcgc
     9421 acgttcagca ccagaccgta cggcaggatc agcggatact cgagtacctg gagcctcggg
     9481 tccttccgga cgaagggcac gtggatggcg gtaaccccgg cgaagtccag ctcaccgccc
     9541 accagggccg cgagcttggc ggtcggctcg tccaccgtga cgacgatgaa ccgggagagg
     9601 tgcggcggcc cacccagcgc agccgggaag tcgggattgg attcgaagat ccaccggcga
     9661 ttgtgctcga acgacacgaa gcgaaagggt ccgttcccga tcgggtgggt gttccagggc
     9721 gcgctccgca tctgcgcgtg ggggacggag ctcagaagat gggcgggcaa gatccccagg
     9781 tccgtaaaca catcggggaa accttccggg ggccgcgacc agcggatcac gaccgtcgag
     9841 tcgtcgaggg cggtgacgct ctggatcgcc gtgagatccg cgtaccgcgg atacccggtg
     9901 actgagtcgc gcgccgcgtt gagcgtccag gcgacgtcct tcgccgtggt ttgaacgccg
     9961 tcgtcccagc gcacttcggt atgcagggcg aacgtcagcg tgcggcgatc cgcgctccag
    10021 gtccaggcac gggccagata cggctgaggt tgcagtgcac tgtcataccg ggccagcgtc
    10081 gtcagaagcc cgtaccgctc cacctgccgg ggcaacggcg agcgggtcac gagactgttc
    10141 atggtttcga ggtcggcgcc gtacgcgaag agcaccgttg atccgcggcg ggccgcgcgg
    10201 tccccggcgc acgccgcggt aagacccgcg accacgagga gccaccagcc ggggcgatgc
    10261 acgcccgcat tctagcggcc ctcgaaccgg gacgcgagat tgtcgccgtg ctccggcatc
    10321 tgctctcacg cctcgcggtg ggcgccgcgg tggtcgcggg cgtcgtggcc ctgacgtttc
    10381 tcctgctcca tgccgcgccc ggcaacccgg tcgactacct gctcggcccc accgccaccc
    10441 ccagccagat ctccgccgcc cgccactccc tcgggttgga tcagcccctg ccgaagcagt
    10501 tcgtctcgtg gctttcccgc ttcggtacgg gcgattgggg cacgagcatc gccaccggac
    10561 ggccggtccg ggaaatgatc gccaccgcct ggcccgaaac ggcgtggctc gtcgggttgt
    10621 cactcctcct gtcgtgcggc cttggcgtgg tggtcggcgc actccaggcc gcgcggccgg
    10681 gaacccgcac ggacgcggcg ctctcgatcg gcaccgtgac gctctacgcc gtgcccagct
    10741 actggctggg gctcatgctg atcatgctcg gcacgtatca gtggaaggcg ttccccgcgt
    10801 tcggtgcggc cggctacgac gccgatttcc tgggggcctg ggcccggttc ctcgatcggg
    10861 cgcgccacct cgccctcccc ctcttgaccc tcacgctcat cgggatcggc ggaatggcgc
    10921 ggttcgcgcg tggcgccatg gtggacgtcc ggcgggaacc cttcgtcacc gcggcccgcg
    10981 cgaaaggcct tccgccgcga ggggtcacac ggcacatcct gcggaacgcg ttgattccgg
    11041 tcgtcaccct gctggggctc tccctgccgg cgctgttctc cggcgcggtg ttcgtcgaag
    11101 cggtgttcgc gtggcccggc gtgggccggg tgctcgtgga agcggtgcag gcacgcgact
    11161 atccggtcgt catggcggcc acggccgtca gcgccgcgct cgtcgtaatc ggaaacctgc
    11221 tcgcggacct gtgggtgaac gcactcgatc cccgcctcca gaggcaggca cggtgagtcc
    11281 gacttggcgc gaccgtcgcg gccgcttcgg catgaccgtc ctgctcgtcg cagtggccgt
    11341 agcgctggcc gccccgctcg ttaccaacgg gcacccggac ctgcaggcgg acgtcatcca
    11401 gacgcgcttt ctcccgcccc tggccacgga cctgcacggc gcgtggcacg tcctgggcac
    11461 cgaccggttc ggacgggacg tctggacccg gctcgcgtac ggtgcgcgca tttctctcgg
    11521 ggtgggtgtc ctcgccgtcg ccatttccgt tgcgctcggg tgtttggtcg gtggcgtggc
    11581 cggggcgagc cagggctggc tctcacgcgg gctcatggcc gggaccgact tcgcgctcgc
    11641 gcttccccgc gtggttctcc tgcttctgct cgccgctctc tggcgcccct ccgcgggact
    11701 ggtcgtgatc gtactgggag tcacgggatg gatgggcatc gcgcgtctgg tatacggtga
    11761 ggttcgctcc ctgtcgacac gcggttttgc ggagaatgcc cgcgcgctgg gtgccacgcg
    11821 ccggcgcgtg ctttggcgcc acctcctccc caacgccctg acgccggtga tcgtcgccgc
    11881 ggccctcggc gtcggcaacg cgatccttct cgaagcagga ttgtcctacc tgggactcgg
    11941 cgttcagccg ccgacggcgt cgtggggatc gatgatcgcg tcgggccgcg acacgctcgt
    12001 caacgcgccc tgggtcgcgg cggcgccggg catcgcgctg gtactggtcg tcgttgcctg
    12061 caccctgctc ggagacgcac tccgcgaccg gctggcgccg cacaccacct aggcgcgcgc
    12121 gagctcggcc agcacttcgg ggagcacttc gtggtggccc gggaagatct tcacctcggg
    12181 atcgatgccg accttccgcc agaggcccgc ttccgtctcc cgcaccttgg ccgttatcag
    12241 ctcgtcctgg tctccgcaga ccaccgtgac gcgagtcctg gcgaagaccg acaggtcgac
    12301 ctcggggggc acggtcccac cccacaccac caggcgcttg acgggcgcgc gcgtccgtgc
    12361 ggcccaccgg gtcgcggtcg ccacaccctg cgaaaaaccg agagcggtca ccggcagatc
    12421 ccggccgatc cgacctctca cttcagcgta gagagcgtcc aggtacccga ggtagtcctc
    12481 gatctcatgg agacggtctt cgcgcgtcat ccacgaagcg cccatgactc gctgctgcgt
    12541 ccggtcgatg tagaaacgtg agagcccctc gggggcgacg accatcctcg tcccatccgc
    12601 gaggccttcg aagtcttccg cgaaccggcg cgccagctgg ccgtagccgt ggcagaccat
    12661 ccagagctcg gacaatttcg gccccggttc gccgagagtg gcatagcggg ccgtgcggga
    12721 gacgcggatc gaacgctctt ccataatccc ctaggtaatg agcagtgtga cgatctcgtg
    12781 cggctcggcg atgaaccgga cggtatgacc gcggccctcg acgaccagcg gctccgccga
    12841 cttctcgtcg gcccggacgc ggtgcgcggt cctgatggta tcgggcagcc gccacgcgcc
    12901 caccacccgc tccccgcgtg cgttgaagca gcgcaggacc atccccgagc ccgtttgcgc
    12961 cggcttcacc gccgagagca ccagcccacg cccgtccagc gtcgcaccga acgccttggg
    13021 cccacccagg ttcgcgtttc gaagccaccg cgcgcgggtg ggcaggaaca catcctccca
    13081 cagctcgggt agcgtctctc ccgcatcgag gtcgcgtgcc ccgacggaca ccagcgccag
    13141 ctcgatgcgg tcgcggccga ggcactgccc gtccggagtc gaggtgggcc acccggcatg
    13201 gccggggcgg gtggggaggt cgtctttcga aagctccccc gctccgcgca ggatcgtgaa
    13261 ccgcaactcg ccctcgggag tccattcgta ctcgaagaac cccggggcca gcagggcgag
    13321 tccccggttg ccctgggccg cggccacgaa gtggtgaccg ggcgcggtgg tcacgggagt
    13381 ctcgcgcgga tagtcctcag cgtgaaccga gaccgggccc cggcggacgg cgccgaatgc
    13441 cgcgcccgcg agcgcgggtc cggtgacgcc gacggggcag cacagacgca ggcggtgatc
    13501 cgcgacccgg ttgtcgatgt cgagcagaca tctgaggacg gggctttcgt cgaagagctg
    13561 caggaccaga cgggccgccg ccccgggcag atcgtacgcg acctcgagcg cggcgaccag
    13621 cggccctccg gccagtcgcc ggacgcgaat gggtccgccc gcacggaccg gcctcccctg
    13681 cggaaacggc gtatagagat cgccgaggtc ccgctcctcc tccagccgca ggagaccctc
    13741 gaatcgctcg ttggtccggc ggtcgagaag ggtcacggcg cctgtcggct cgacggtgac
    13801 gtcgaggaac gcattcgaca gcgtgcgccc gtgcaccccc gctcccgtgt cccgccggag
    13861 gggagcggag acgcgcgagc ggagaacggt ggcgcgcagt ccttccagtg ccggcgcgtg
    13921 gaacgcgatc cggatatggt cgacttcgtc ctgatccgga taatggcgcg ccgcatcccg
    13981 gcgttcgaga gccttccgcc ggctcaacac ctggatggga atcgcctccc cgtcctggtt
    14041 catcaaggca aactctccgg gggcgtgccc atgacggggt ttccgatcac cgggcggccc
    14101 caccagaata tcccggcgga accaggtcgt atcggcgatc accacgccgc tccgggtgcg
    14161 cgcgaccggg ttccagagcc agagcgctga atccggatgg ggatggtccc gcgacaggtc
    14221 ggggtcgtgc cccgacagat cgtccgccgc gccgtccgcg agctcacggc tggtcgcttc
    14281 gacggcatcg aggcgagtgc cctgagcgcg cgcgaccggg tcggcgcagc atccacagag
    14341 cgtgtcgtgg aacgtcccgc gaatgagcgt gcgccaggcg cggtcgagaa gcgcccgccg
    14401 gtcccgccca cccgcgcgcc gggcgagggc ggcgagcggc tcgacgatcc gctcgagctg
    14461 cagctccgcc cgcgaatgcc gccgcttcaa tggggcccgc gtcgagtgca cgccctgcaa
    14521 ggtccaggta taccggtacg accagcgcaa ctctcccgac acttcgggcg catgctccgc
    14581 ctccgtcgcg agctccttca ggaactcatc gagacgggaa atccggacgg cgttctgggg
    14641 ctcgagctcg gcgatccgct cccggagcgt cggcaggtcc gggtacggcg catgatgatc
    14701 ggcgccgacc ggtacggcga tgtgattcgt cgccgcccgt cgcgtgagct ccgcccggag
    14761 cggcacccag gcctgccgca gccgctcgcg gtccgtggga agcgcggcac ccgcctcgta
    14821 cccgtcgggc ggaaagtgcc agagcaggag gtcgcgcccg tcggggcctc gccaccggta
    14881 ggcgtcgcgc gtctgccccg gttctccacc gagcccgcgc cagagcgccg cggccttgag
    14941 gccgaactca cgcgcgagca cggggaggat ggcagggtga ccgaacgcat cgggcgaata
    15001 cgccacgtcg agacgcccac cccagcgggc cgtgtcgatg gcgccgtaca ggagattccg
    15061 gatcagggac tcgccggagg gaatcagctc gtcggccagg acgtaccaag gaccaagctg
    15121 caagcggcca ctctgggcca gcgcgcgcag ggaggcttcc cggtcgggcc ggacggccag
    15181 gtagtcctcc accagcaccg tctggccatc gagcaggaag gaccggtacg cgggatcact
    15241 ctcgagcagc gccagcaggt cgtcgaccgc gggcaccagg cgcgccgtga aggccgcgcg
    15301 tgggaggtac cactcgcggt cccaatgggt gtgcggaatc aagtgaaacg ttagcgtcac
    15361 ggcccctccg gcccggagga tcgcggtcta tattccgcga atccaggcac tcgaggattg
    15421 atttgtgctc cgcaagatag cgctctccgt ctcgggactt ctcgtcgtcg gcgtagccgc
    15481 gcaccggatc gtgccggggg ccctcggcaa cggcactacg gtcctgccct caggctggcg
    15541 aatctccccc gccggcaagc aggtccccgt gggcaccctg cccctcaata tcctccggct
    15601 cgcgaacggg caggtggtcg tcacgctcaa cggccattcg gccaatggcc tctggcgcat
    15661 cgacgcgggc accggaaaga tcgtcgactc actccccttc aaagctgcgt ggctgggact
    15721 cgcccaccga ggtacggcca cggccgatac ggtctgggcc tcgggcggca gcacgaaccg
    15781 cgtctatcgc gtgatctatg gctcaggtgt ccccgcggtc gacaccgtcg tcctcgcgcc
    15841 gccgccggcc gccattttcc ccgccggaat cgccattctg ccgggccggg acctcgtcgc
    15901 cgtggttggg aacctctccg actcggtcta catcctcgac accaggaccc tggcggtgac
    15961 cgcgaagttc gccgtcgggc accaccccta caccgccgtg gcggattccg cggcggtgta
    16021 cgtgagtaat tggggagatt cgagcgtgga acgcctgccg atcaccgcgg ccggcgtggt
    16081 ggacggcgcg aagttggcgg tggggccgca tccttcttcc atggtgctcc gcgggcatca
    16141 gctcttcgcc acgttggcgg gcgccaacga agtcgcgcgg tacgatctcg actccaaccg
    16201 cgtgacggac cgcctggcgg tcgcgctgat gcgcggtgct cccaccggca gtgatcccaa
    16261 cgccctggct ctgacccccg atggccggcg gctcttcgtg gccctcgcgg ggaacaatgc
    16321 ggtcgcggtg gtgaatgtcg cggccgcaac gccgacggtc gagggactcc tccccgtcgg
    16381 ctggtatccc accggcgtgg aagtctcccg cgcgggcgac acgctctatg tcgcgaacgg
    16441 caaaggcaat ggttcgggac cgaacccctc aggtccgtac atcgcggacc tggtccgggg
    16501 ctccgtgtcg gtcatcccac tccccgacgc ctccgggctc gcctcggcga ccgcgaaggt
    16561 gttgagcctc tcaccctatt cgaatcccgg actgcgcgcg gccccgcccg acctgccgaa
    16621 gattcgccac gtcgtctacg tgatccggga gaaccggacc tacgaccagg tgctcggcga
    16681 tgcgaagcgg gggaacggcg atgcgaagct cgcgatcttc aacgattccg tgacaccgaa
    16741 cgcgcacgcg atcaccagcc atttcgtgtt gttcgacaat ttctatgtgg atggtgaagt
    16801 ctcggcggac gggcacgagt ggaccgatcg cgccttcgcg ggcgagtaca atgagaaaac
    16861 ctggcccgcc gtctactcga gccgccggga ttgggatctc gacgacggtt ccgacctggc
    16921 caacccccac ggagcctacc tgtgggatgc cgcccgccgc gccggtctgt ggacggtgaa
    16981 cttcggcgaa gccgtgcaca cggactcgag cggacggcac gtttcctcca tgcccggact
    17041 ccagggcatc accgtgcccg agttccccgg cttcgagctc gacatccggg atacgacgcg
    17101 ggccaggctc ttcgccgact ccgtgaacgc ctgggatcag cgggacacgt tccccgcctt
    17161 ggtgattctc tacctgcccc gcgaccacac cgagggcaag cgggccaccc atccgacacc
    17221 gcgtgcgatg gtggccgaca acgacctcgc gctgggtcag gtcgtggagc gtctctccag
    17281 atcgaaggca tggaagagca tggcgttctt cgcgctcgag gatgacgccc aggatggacc
    17341 cgatcacgtg gatgcgcacc gctcggtgct catcatggct tcgccctatg cccggcgtgg
    17401 cgtcgtggac agcaccatgt attccacgag ctccgtggtg cggacgatcg ggatctttct
    17461 tggactttca ccgctctccc agtacgacgc gggagccaca ccgctctcgc acgccttcgt
    17521 ctcgcgggcg gatctcacgc cgttccacgc cgtggcaccg cgctggccct tcgacgagct
    17581 gaacggcaag caatcgatgt cgcggctcac cgaccaggac cttgcgggcc ccgacttggc
    17641 ggatgaaacg attctgaatc aggagatctg gcagagcgtg cggggacgcg aggcggtacc
    17701 cgcgccgcac cggacgtacc tgccgggacg tgcgacggcc ggagatgacg acgaccgctg
    17761 accggccgtc aggcgggtga gagtacgctc caatcgtccg ggtctcgcag atgcacatgc
    17821 gagacccggc gtgctttcgc caggagcgcc ttgatgtccc cgacggcctg cgcgcgctcg
    17881 agcttggcga acgtggtctg catctcgggg atggcgatgt cctgatcggg atcggcggtg
    17941 acgacgagga agagcccggt gtcggggcct cccttgtgga gctgccccgt ggaatggaga
    18001 taccgcggcc cgtatcctgt cgtcacgacg catccggtgg cgccgtgcca cagcccggcc
    18061 agcgcgccga gccgcgcatc attcgcgggc gtctggggga ggtacgcgag aatcgcgacg
    18121 tagtcccccg gcctggcgga cgaggcgagc gccttcggcg acagtgtgga cggcgacgcc
    18181 tctgcggcgc cgccggggcg cgccagctcc gcccgcgcga gcaccttggc cgcctccacg
    18241 tccggctgat cgaacggatt gacgtcgaga gcttcgcaga tcgcgacggc cgcgtattcc
    18301 cacgtcagga aggtggcccc gagtgacacg ggctcggcgg cgccgatgtc ggcggcgaag
    18361 acgtccttgg tatgccaccc cgcaccgagg accggtacca cgccgcgtcc ctgcttgccg
    18421 gtgctctcag ccaccagctg ctcgatccac gcggccaccg aggatatcgg cccccctccg
    18481 cgaagcgcca gcttgtcttg accgtggcgg gccgccgccc cgatgctggc gccgagttcc
    18541 cgggccttcg cctcgtccac cgccatcgcg ctctcgagca ccgcctggcc cgggccaccg
    18601 atcaaggcga gcgggagcat gccgacgacg gtcagggccg catatcgccc gccgacatcg
    18661 gctggatgcg cgaagacttc gcgccagcgc tcgccgcgcg ccttgtgttc caatggcgtg
    18721 cccggatcgg tgatggcggc gaaatggctg gaaggggcga gacgggcaaa gtggtggcag
    18781 gccgccatcg actcgacggt cgtaccggac ttgctggcaa tgaggtagag agtctgcgcc
    18841 gggtcagacg cgtgttcggc cgcacgaacc gaggccggat ccgtactgtc caggacggtg
    18901 aggttcttcg cgccgaagct cgaagcgagc acccagggcg cgagactcga tccccccatg
    18961 ccgcacacga cggtgtgcgt gaggccgtcg gcccgcgcgc gggcggcgaa ctcggcgagc
    19021 cgcggccatt catgcaggga tttgcgggga gcctcgcgcc agccccagcg atccggatcg
    19081 ggagttgcca tcgaggcgga agatagccac ctctccccgg ctccgcgtcg gacgcgtact
    19141 ttcgaaggcc gtgacccctc cagccgtacg ggccgatctg gatcgccgag gtctctggat
    19201 cgcgataggc gcgctcgcgt cttacatcgt gatgtccgcg gcgtatctcc tgaaccccgc
    19261 ctcccagaag tccctggccc ccgtcgcgtg gacctgggcc tccctgtatg ccaccatcgc
    19321 cgccctcgtc gcccggcgcc aggcgggcga gagcgtggaa cggcgggccc tcgggtggat
    19381 tgccgcgggg tgcgccatct ggctcggcgg tcagctcgga gatgactggt cgtacttgaa
    19441 ggatgccccg gtgagcctcg ccaagggcat cgccgccgtg gggtacgtcg gcatctatcc
    19501 gtgcctggtg gtcgccgtcg ccatcttgct ccggccgcag ctgcgccggg tctccggcgt
    19561 ggccgtcatt ctcgacaccc tgctgctgac gttcaccagc ggcatcctca ccttcgagct
    19621 gctcagtgag caggggcccg tcaccgattt ccggcgagcc ttcctgggga tcgtcggggg
    19681 tctcggcgcg ttcgtcctct tatggagcat tctggtaggc gtactcaacc ggacccgaac
    19741 gctccaggag catgggtccc gggcgttcgg cgccctggtg gtgttcgcgc tcgggagcgt
    19801 cgcgtacgcc gcggcccaga tcctgaccgg ggagatcggc gcgcagcggt tgcccctgtg
    19861 gatcgtcacc ctggggtgga cggctctctt ctacatcctg gccgcgggca tcgcctggca
    19921 aacgcccctt tggcgcgctc gcgcgttcac ggacgcaaag acgggtgtcc tgtcacacct
    19981 ggtggtgctg atcgccggac tcggcggcgt gttcggcgtc gcggtcatcg ggattctacg
    20041 gcccggcgtc gacgtgcccg cggcaatcct ggtcggtgcc ggcgggatca tcatcgccgc
    20101 gcggctcgtg cttgccctgc gcaccgatca ggagtacgcc cgccgcctgg agcgggacgt
    20161 ggaacgccag acgcggtccc tgtcggaatc gtacgcggag cagcgccgga tcgaagcgca
    20221 gctccagcag tccgacaagc tggcggcgct gggacagctc gtgagtggcg tcgcgcacga
    20281 gatcaacaat cccgccgcca tcatctccgg gttcgcgcag acgatgctgc tcgatgagct
    20341 ccccgccgag cagcgtgaga tggccgagat ggtccgggac gaggcgatgc gcatcgggcg
    20401 catcacgtcg aacctcctcg cgttcgcgcg gtcgggtggg agcgagcggg acttgatcga
    20461 cgtgaacgac gtggtgcgcc ggacctacgc actgcggtcc tatcatctca acacactcaa
    20521 catcgccgtg gcggtggacc tgtctcccga gacgccggtc gtctggggga atggaccgca
    20581 gctccagcag atgttgctga atctgctgat caacgcggag caggcgctcg ccgcacagca
    20641 cggaaaccgg tccatcgcgg tacgcaccgt cgcggcgccc gacaccgtcc gcatcgaggt
    20701 tgcagacacc ggccccggtg tcccgagcga gatccaggcc cagatcttcg acccgttctt
    20761 caccaccaaa cccgtgggcg tcggcaccgg gctcggactg tcgatctgtt acggcatcgt
    20821 ccaggaacat ggcgggcgca tcgtggtgga ctctcgcccg ggccaaggcg cgacgttcat
    20881 cgtccagctc ccacgggaca cacgtcccct gcccaaggca accccggaat ccgcgtcaca
    20941 gacggcagtc gatcccgccg gccttcgggt cctcctggtc gacgacgaac ctgccctccg
    21001 cgaagcggcg cgccgtttcc tcgagcgctc gggtatcgtc accgacgtgg cggcggatgg
    21061 tcaggcggcg ctcgaggcac tgggtgcccg ggcctacgac gtgatcatca gcgacgtccg
    21121 catgccgggg atggacggcc gggacttcat cgcccgcctg cggaaggacc ggcccgagct
    21181 tctcgaccgg ctcattctgt cgaccgggga caccttcgcc gaggacacgg cgggtctggt
    21241 ggcggagacc ggcgtaccca ccctcgtgaa gcccttcgac ttcgatacgc tcgaacgaat
    21301 cgtccggaaa acagccggcg cgcgctgaaa gcgtcaaata tctagacagt tctccgcgtt
    21361 gccactttga gcctatttcc ctacagtgtc gtgacttctg tgaagttaga tgtcaaatat
    21421 ctatacgaat cgtccagcac cgtttgttcg ttgcagatct ctatctagct gtgtagcaac
    21481 gacatacgta gcatcaggac aaggtacgcg atgtgcctta tggggtgggc gagcagttca
    21541 accgcgccca tacctgaagg ctgacaatga ctcgcccggc ccttgtcgtc gccctcgctg
    21601 tttcgaccct gctcccccgg atcagctccg cacaggcttg ctccgccaat aactgccgcg
    21661 ccacgaacga ggtcgtcgcc acagtcggcg cgctcatggg cctgtccttg gaccggccgg
    21721 cggccgtgac ggtgaatccc tccgcacggg acatcgccac gggtgctcag gagctgagcg
    21781 gaccgacggc ccgggtgaag tcgaacacgg attggcggct cgaggtcagc gcggcgtcga
    21841 ccgagtgggc cgccggtcag ggcgcccgtt cagggaagcc ggccacggac ctgggttggc
    21901 gggtgaacgc gaacggcggc tacacgcccc tgtcgaccga cccggcacag gccgcgaccg
    21961 gcagccatac ctcgaatacg gaagtcgcct tccagtatca gacgtcctac cacagccaac
    22021 aggatcggcc ggggacgtac tcaatggtgg tgcggtacac gcttacgacc cggtaagcat
    22081 gacgtcgagc acagcggctg gttcccaccc tgaagggtgg gcccggtacg acgtggcgct
    22141 tcaagcgcta tgcagtgtgg ggcccaagct tcagcttggg ttcgacgaat gtggaggctt
    22201 cagggcgcgg cgagcgtgaa gacgacggtg agggagtacg tggcgggcgt atcgagcccc
    22261 caggcgtact tggtctgata gaagatggac accggcgcag acccccccgt ggccgggatg
    22321 gtttggaccg tcgccggcgt cgtcgtcaac cccacgaagg tcccgcctgc actcgtactc
    22381 cacgtcaggt ccgcgcaggg cttgctccgc ggcgtcaccg aggtcgtcgc gacggcactc
    22441 cacgtcgccg tcgcggcgct gatctgcaag cggcaggtga cgttgcactg cacggtggcc
    22501 gacggtcccg tgttcgccac gaatcccgcg tcatagtcgg ctgaaccggg cgccgtcagg
    22561 gccgtggtcg tcgtggagag cgagagctgc acgacggcgt tcaccggcat cgtcgccgtg
    22621 acgttcaccg agcaagtccc ggcaccccca ctgagcgacg cggtgcaagt ctgtgcggcc
    22681 agcacgcgtc ccgcgccgag gagcaccacg gcaatccaga gaccccgctt catgggccgc
    22741 tccggcgaac ggccgtgaac gatcccgcga aggtgcccgc cccgccggtc tccacccagc
    22801 ttccgcgcat cgtgtcgcgc acgaccgccg ccacgtggcg tcgtccgttc ggatccgaat
    22861 tgaagaaaat gtcgaagtcg acggtcgtgt cgttcacggt ctgtccggcg atgggacccg
    22921 agaccggggt cgtggtactc ccctcggtct ccgagcccga agcgctgccc tgaaagatcc
    22981 cgttcgactg cgacgacacg atcaaggtcc cggtgatgtt cgcggtcgtc ggagtcgact
    23041 ggctggccgc atagtcccaa gtgccggtga cgaccagcgt cgacgacccc gtgtcggtgg
    23101 aggtcacgca ggccgcgagc agaatgccgc acagacccag cgccgggttc atccgtgtcc
    23161 cttggtggcg ccgcctcggc cccgctgatt ggtggggtcg aaaaggcgca cgggccgggg
    23221 atagacggga acccggatgg cagggaccgt cgcaccaggc ttcacgacga acgtgggtgc
    23281 gggaccggcg gtgtgcacgg gttcgcggac atcctgcagc tggaagtcga ggtggtaggt
    23341 gcccggaggc agcgcatcga aacgcgccgt gccggtggag tcggtccgtg cggaccagac
    23401 attccccgcc gagtcgaccg cgttcacgag cccgggccgg aagtcgaccc gaggcacccg
    23461 ccccgtttcg tcggccgtcg gcacgagccg cacctgcacc gacgccgtcg cccagatgcc
    23521 gatctctacg cgccgctggt cgggcgactg gagctccgat gccggattcg cgacgtcccc
    23581 gaacggtaaa gaagtctcgt ccacacgcag ccgttccgcc gtctcctgga tcagcgtgaa
    23641 ttccccgttc ttgtcggtga cggtggtctc atcaccccgc cgcaccacca cgccagggac
    23701 cccggcttcg ccgcggtccc ggacgccgtt gccattcccg tcctcgtata ccacaccgcg
    23761 caccagcgca tgactcgaac cggttgccac cgagagcgtg tgttcgagct tcacaaccgg
    23821 cacccagcga ttgcccccgg tggggcccgt cagcaacgga ttgtgctgga cgtccacggt
    23881 cagatggaga tcctggggca gcggcacgac cacaccaaga cggaccaggg tcgcggacgg
    23941 cagagcgccg aaccagagat actgctggac ttcggcgtgc acgcgggagt tgccccagac
    24001 cggcacgttg tcggcgcgaa ggctgagcag cgcttcgtgc gggagatacc cgacccgggc
    24061 ggcgctctga tcatacgaga cgcccgcttc gaacagtccg cgatccgtcg aagcgcccat
    24121 ggtgccttcg atcccgtagc gcccgccggc gtcgacgatc gaaggcgcgt tcggcaggtt
    24181 ggtcgtgcgc gagacctgcc ccgccgtgag gcccccactc gcgtagaagg ggccgacgtg
    24241 catcgcggcg cccgcccgga ccgccgtctc gttcgagccg aacgacccga cggcgccatt
    24301 gacgctgtag gcgctgccat gcgcgtcgag cgtgagggag aggtgactgt ccacctcgta
    24361 ccgcggactg acggaccacc ccaccgatcg caggctgccg aatccctggt tctgatcggt
    24421 gctcaggaat gccgacccgt tgagctggag gttgccgatg gtgtgcgccg cctgagcgtc
    24481 gaactcggac ttcgcccgcg catacgccgc cgcaccacct ggtgccgaga gcgcgcggag
    24541 ctggaactgg ttgtcggccg agcgctgatc gacttcggct gaccacccca tccccgaccc
    24601 ggtggcgtag ctccggtgcg cgagctcgcc ggacagcgtg aagcccgaga aggcgggaga
    24661 catgccgccg acaccgatcg cctggagctg ccggggtgcg aacacgttgt cctggagatc
    24721 cgtcgccgtg gcaccgaccc aaccgccatc gacgtgcatc ccgatgcggg ccccatagag
    24781 gtgcccgctc gacgactgcg tgccgacgct cggcgacgcg ccgaggacgt cggccgtgta
    24841 gcgggtatcg tcgaaggaga aggatgcgcc gcgtccgtag gcgttgagcc ccgtgatgtc
    24901 ggagaaagtg tgcccggtgg cgccagcggt cgcccgccag tgggaagcgc cgaccgacag
    24961 ataggagccg cccaagtagt atcccacccg cgcgagcgcg atgggatcgg ccgtcgccgt
    25021 attggtcgcc tggatgaacc gccccgacat cgtcagccca ttcgccagcg gcccggtcgc
    25081 gtccacttca aagacgggcg cggaggtccg gttcgtcaga accgacgcga cccccgaatt
    25141 gacctgcaaa gatggaccgc catgcagcgc cgctccggcg acgacctcga ggccggcgtc
    25201 cgcgcgcgcg agatcccgcc cccccgccga cacgatgacc cccagacgca cggagccgat
    25261 gccggcgtca ctcggcaccc agagcgtaac ctcctcggtc gccgtggcgc cggcggggat
    25321 gacgatggtc gtcggcgcgc cgacgacgcg ccaacccccg ggcgcgagga gacggaccgc
    25381 cagggtgtcg gtcgcgttgc cggtgttggt cacgaagtag cgcagcggga tccggtcgcc
    25441 ggcgtgcccg ccgaggaccg tctggccgag gcgaatgagc gcgctgtgga tcgtcaggac
    25501 gttcaggcgc accaacacct cgccgcggac ggcgccactc tggctgaatt gcacccgcgc
    25561 gaccgtctgc acaccggccg gggcgctctt ggacaagttt gccgccacca cgacgggccg
    25621 gttcgcttcg ccggacgtca ccgccacggt gccgttcagg gcgccgacca ggtgagcatc
    25681 cccctcgggg acgacttgat actggacgtc accaccgctg agaagcgccg ccggcaccgg
    25741 gaccgcgacc acctgaaccg accgtggcgc ggccgcggcc tcgaccggct cgaggtcgag
    25801 gtgggcgacc ggggctgccg gagcaatcgg ggtcaacgcg gcgggggcct cgtgagcagg
    25861 cgcgacccgc tgagctgggg ccgcggccgg ggtcaatgtc gcccgacgga ggctcgtccg
    25921 ggtcgcggga gcggcaggat tcaccgtttg gatcagcgtg tcaggtcgga tcacggccgg
    25981 gaccggcgca gcggagggag cggcggccga gacggagcgc cgggcgccgt gccgaatcac
    26041 attgcgcagc gtcaccgtcg cgcccgcagc cgcggccacc agggtgaagc tgagcgcccc
    26101 aatcacgacc gaacgcttca tggcgtggtc accgtcaggg tgatcacgag ggtggtcgtg
    26161 taggccaccg cgggtggaga cggcggcgta ttggtccaac cgatgaggca gcggaagaga
    26221 aggctgttcg accagttgtt cccggcgttg cggctgatct ggtgagactc gaccgtcacg
    26281 tcactggtgg tcaaagattg ccacacgccc gagccgctga cgctccactg caaatcgctc
    26341 agcgccgttg ggccgaacgt ggccgacgcc gaccgcacgg acaccgtggt cgtatgcagg
    26401 gtgctcggcg gccccccggc gatgttcacg ttatacgaga gagcgctggg atcgagcacg
    26461 aaaccggcgt tgtagtccgc gacggccggt gccggaatcg ctaccggtcc tcccgacacc
    26521 gtcagcacga cgctcccctg cgcaaacacc cgcgcgggga gcagaacggc cagtgccagg
    26581 atcgcgcgcc gcactagggg acgtccagcg gcacctggcc ggccgcgact tcactcccac
    26641 cgaagtcgag gagggccaga acgacgtagt gtcccttggg cagcgcgggc aggggcaccc
    26701 gggctcgacg ggccgcgccc ggcaggacgt agaaggtcgg gaccgaatcc cggtgcacca
    26761 ccgaattgtc cagccggcgg tactcgatct cgccctttac gatcgctggc agtccgccgg
    26821 tattccggaa ctgcaccgac gtcacgaccg tatcggcggc cggcggaacc ggcttggcgc
    26881 ctttcacggc ggggacgcga acggcgtgaa tggtgtcgat ccccatgccc tcgacgtctc
    26941 cgtcaacgga caggccgggg ggctcgatgt acaccttcac gcccaagcgg aggacgtatt
    27001 gaacttggcg gcccgcgccc gcctgcggtg gagcggcttc cacgaacacg atgctccagc
    27061 acgtggcggg gagcgaatca gcgccctgga ggcccaggcg cacgccctgc gatgtatgcg
    27121 gcggaagccg gaggctcagg gggaacacgc ggagatactt cgcgcacgac tgcgaaacgg
    27181 tcccgagctc gaggaagcgg ttgttgccat cctcgtcgcg gtcccagtcc ttcaggtaga
    27241 ccttcgcctc gaccgtggtg ggggtctcgt tcgagacgtt gaacccggcg acggccggcg
    27301 acgacggctg cgggtgcagc acgatttccg cctggtccac cgagacctgc gcggtggccg
    27361 gagcggccac cgccagaccc cacacagaca acgcggcgag tacgctccag cgcacccgcc
    27421 gcgtcgtcag agagaaaacc agcgtcacgg ctcggtcagc gtgtacacga cggcgagcga
    27481 ataggcgccc ggcgcatcgg tgctgtgcca cgcggcatga tagaagatgt tcacggactg
    27541 gctgccaccg gtcgtggtct ggctgatcac gttcgcgccc gtggtcgaca gcgcggcata
    27601 ggtgccaccg gcggtgaggg accaggacag gtcaccggcc gacttcgtca cacccgcggg
    27661 ggcggtgaag ttggcggcgc cggaagcgat cgtcacgctg aacggaacat tggctttgcc
    27721 ggtcaccgag ggacccgcgg tctgcacggg gttcgcggcc gaatcgacgt tgtcgatcgt
    27781 cggcacgccc agggcggtcg tcgtcgcgct cagggtcagc ttctgcaggg tcgggatcgt
    27841 gaccgacgcg gtggtgctga ccgagcagcc cgcggtggtg cacgtctggg cgctcgcggt
    27901 ggccgagaag gccaggccga gaaccgcagc cagagcaaca gtgagacggt tacgcatcgt
    27961 gtgctcctta cagggtaagt gttcacttca agaaccagtg agtccatctg ccactgtcta
    28021 gctaaggcaa cgggtgtgcc aactgccgaa aggcacgtca ctgtcaaatt tttttacgta
    28081 gggagcctaa aaaggggcga gattatagtg cggattttat taaaaaacaa gattttggtg
    28141 attctgggtg ttcacaatga tcagtattcg gccaatacca tccaatcccc taaaaaaacg
    28201 cccctcgcac cttgcggtac gaagggagaa ttgcggcgtg atctgggagg ctcaggccct
    28261 gggacctgcc cgccgacgct ccatgatggt ccgccgctcc gaggacgagc gacgtaccgc
    28321 cagccggcgc tccatatgcc ccgcccgacg gtcagcccca gctcgccgtt ccaccaccac
    28381 cgcgaccacc accaggcgcc gatccccctg gaaacgtcga tccacacccg cccggcgatc
    28441 ctgcaggtcc cggcgatccg cggctttccg gcgattcaga gggggcatga caaccatacg
    28501 gatgctcctt cacagcattg ggttccaggg aaggctaacg tatcgcccgc aagccgcccc
    28561 cgccagacag gggcccggga agcgtctatc ttcctgtacg acaatggata acatcgcgca
    28621 ctccctggtg ggagccgcca ttggacgcgc cattggcgac tcccggatcc ccgcccccgc
    28681 cctgttgggc gcccttgccg ccaacgcccc agactacaac gaattcctcc ccgggatgcc
    28741 cccccggtcg aacttcaacc accgcgggat tacccactcc ctgctgggag ccgtaatcca
    28801 ggccgccgtc ttcgtgatcg tcaccggcct cgtcatgctc cccttctgcc ggcgggagaa
    28861 tcggcccgtc ccgtggaaga ccattgtcgg cctctatggg gcggccctcg ccagccatct
    28921 gctgatggat tggcagggct cctacgggct gcgacccttt ctcccctgga gcaaccagcg
    28981 ctactacggt gattgggtcg ccatcgtcga tccgttcttc tggctggtgc ccgcggtgtt
    29041 tctcgcctgg ggccacgtgc gccgctggac gggcgccctc ggctgggccg tgctcatgct
    29101 cgccctcgtc gccattgtgc tgttcgcctc ccttttcttc gaccgcgcgg tggccacgtg
    29161 ggtcgacatg gcgtcgatcg caattcccgt catcggcatc gtcggatggc accgccattg
    29221 gttcgccccc ggagccaaac gcaccgcggc cgcgacctcc ctggtgctcc tcggcctcta
    29281 caccggcgcg caagcggtca cggcgatggt cttgaaacat cagctcggca tcgacgcgcg
    29341 gcagcgcttt ggcccccaag cgacctatgc gttgctgacc aggcccggat tccccttcac
    29401 gtggaccccc gtgatgggag gagccgattc ggtcggtggc cccggctgga gcctcccccg
    29461 gcacctggac tcgccggtcg tgcgacgggt cctgaccacg ccgcgggcga aagcggccat
    29521 ggcgtttgat cgattcgtga cagccgatgt cgattccacg agcacacccg tcatggtata
    29581 ccttcgagac acgcacggac cctcggtcgg cagacgcggg ttcggcatca tcgccctcga
    29641 gacgtcgagc acgccctgac cactcccccc cggatgccga tttcccggcc atcgaatccc
    29701 acgacccagg ccctgacgga agcgaccgaa acgcccccgg tcgacgcctt gcaggcactc
    29761 gcgtccaaac tcgacccgat gttccggctg gatcacctgg tcacgctttc gcgcgaccgc
    29821 gccctctatc aggcgtggga ccgcgtattg aagcgattcg tcgcgctgcg cgtgcacttg
    29881 atccccggca cgcccagccg cacgtggttc atgcgggaga cggaaacgct ggcggcgctg
    29941 gaccatccgg ccatccgcca cgcctatgcg gccgccgaaa tcggggacgt ggcgtaccgg
    30001 accgcgaact ggatcgacgg cgagagcttg agcgaggcca tcagacgcgg cccccgcccc
    30061 attccgaccg tcatgtcgct ggtccgcgat ctcctgggcg cgctcgagta cgcgcatgcc
    30121 cacggggtga tcatgcgccg aatactcccc accacgctca tgctcgaaat ttcggggcgc
    30181 gggatcgtca ccgatctgcg ctacgccaac tggtgcctcc cgaacgtgcc cccggacgaa
    30241 caagggggcg gcgcgcccta taccgccccg gagatccgaa gcgggggcgc gggcgaaccg
    30301 gcgagcgact tgtacgccgt cgccggcctc gtttacttgg cgctcaccgg ccaggaccct
    30361 gcagccgatc ccacggccat cgtccccctg cggacggtcc gtcccgcaat tccggcggcc
    30421 gtcgagcgcg tcgtcctccg cgccctcaaa ccggagccct cggcgcgcta ctacacggcc
    30481 accgagatgc tcgaggactt cgtggcggat gcggggtcct tccacgaacc cgcggccgcc
    30541 ccgcagctga tggattcggg gttcgagcgg agactgcggc gtgcccttgg ggatgactac
    30601 gagctgctcg gcgagctcgg ctccggcggc tttggacgcg tgtaccgggc gcgagacctc
    30661 gggctggaac gcgaagtggc gatcaaagtc ctgcacccgt cgctcacggc cgatcccgtg
    30721 gtggtggagc ggttccgccg cgaagcccag ctggcggcgc gcctgcggca cccgaacatc
    30781 gtgagcatct acgacattca gggccgggcg ggactgcagt ggtacacgat ggaatacgtg
    30841 ccgggacaga gcctggccca gctggtgcag gggaaaggac cgctgtccgt cgagcgaacg
    30901 gtccgggtga tgaacgaagc cctttccgcc ctcgagcacg cccacggact gggcttggtg
    30961 caccgcgacc tcaagcccga aaacatgctc ctcgagccgg acgggaggct ccggattacg
    31021 gacttcggac tggccctcgc ccttcgcggc gaccgctacg gcggggcaac gtcgcgaagc
    31081 ggcaccccgc agtttgcggc tcccgagcag ctcctgggtg aacgcgtcga tccccgcgct
    31141 gatctctact ccctgggcgc gacggcctat ttcgcgctgc tggggcgtcc cccattcgag
    31201 ggcacgacac ctgaagccat cctggccaag cagacggtcg acgccctgcc cctcctggca
    31261 gccgagcgga gcgacgtgcc gcgggagctc gaagtggtgc tgcggcgggc ggtgaccaat
    31321 gatccgtcga agcgattcca gcaagccgcg gcgttccgcg aggcggtgct ggccgcggcg
    31381 ggaggattcc ggagaaggct ggtgacggtg ctgcggaggt tggcgggcgc gtgaagcggg
    31441 aaacgagaaa cgggagacga gaaacgggaa acgtgagagc gaacgtttcc cgtttcactt
    31501 ttcccgagtc ccgtcctacc gccagcggaa aatcttcagc gccactccga agcaaacgag
    31561 tccccacgac gcgacgatcg ccagcggggg cccgagatgg aaaatcgaag tgccgtcgat
    31621 catcaccgcc cgcaacgagt cgttcagcgc ggtgaggggc agcgccttca cgaagggcag
    31681 catgacgtcg ggaaaccggg aatacgagaa gaaggtcccc gagagaatcc acatcggcaa
    31741 catcgccaga ttcatcaggc ccgacacgcc ttcgat
//
LOCUS       DASUBP010000318         2551 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_544917, whole genome shotgun sequence.
ACCESSION   DASUBP010000318 DASUBP010000000
VERSION     DASUBP010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 2551)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2551)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2551
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_544917"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="VFU75_12170"
     CDS             complement(<1..267)
                     /locus_tag="VFU75_12170"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HEU5467642.1"
                     /translation="MRISDMNWMQVEAYLKKDDRAVLPIGSTEQHSYLRLIVDCILPE
                     RVAAEAAEPLGIPVFPVLSYGVTPYFREFPGSVSLKVETHLAVIR"
     gene            550..1443
                     /locus_tag="VFU75_12175"
     CDS             550..1443
                     /locus_tag="VFU75_12175"
                     /inference="COORDINATES: protein motif:HMM:NF007214.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma-70 factor"
                     /protein_id="HEU5467643.1"
                     /translation="MSTPDDRDGQFTANRPLLFAIAYRMVGTVMDAEDLVQDAYLRWV
                     EAPETDVRVPAAYLTTIITRLAINFLGSARNRREEYVGPWLPEPLVTDPSPNPEASVE
                     LAESLSLAFLVMLERLAPVERAVFLLHDVFELEYDEIAPIVEKTETNCRQILSRARER
                     VSTERPRFDADTERAEALVARFNQAAGNGDLQGLVAMLAEDITLWVDGGGKAKGALPK
                     PVHGSTPVAKVVINTMKRLAPQGIVFRMARLNGRPGLIAYGAGEAQSAVIFEMGAERI
                     ENIYVVANPDKLQSIKKSVAK"
     gene            1525..2094
                     /locus_tag="VFU75_12180"
     CDS             1525..2094
                     /locus_tag="VFU75_12180"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEU5467644.1"
                     /translation="METYLPPVERPNGLVMKLVYALAKRRFGQVLTPLKVISARMPLA
                     FGTWVGKVSQLDHKLSIQPELMYLIRQKVAQINVCEFCIDMSRWAALQASNSTAKLDA
                     LEEFETSPVFSDAERAALNYVTHLTRFKKVSPAVFNRLAEHFSDRDICEITWVVASEH
                     VYNISNIGLNIHSDGLCRIPSASAVAGAA"
     gene            2178..>2551
                     /locus_tag="VFU75_12185"
     CDS             2178..>2551
                     /locus_tag="VFU75_12185"
                     /inference="COORDINATES: protein motif:HMM:NF025887.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEU5467645.1"
                     /translation="MRLSAILMCLLCTAAITKPPLSDQKIVETLEKQWLAGEHDRATL
                     DTVLAADFVHPVAAGVFLSKAQHIAWAVGHPAPRDRQQQFDQLQVRVYGSAAVVTGMV
                     ISTDLAGHEAKTVFTDVFAKRGG"
BASE COUNT          462 a          841 c          818 g          430 t
ORIGIN      
        1 gcggatcacg gccaagtggg tctcgacttt gagcgagacg gagccgggga attcgcggaa
       61 gtacggggtc acgccgtagg acagcaccgg gaacacggga atgccgagcg gttcggcggc
      121 ctccgcggcc acccgctcgg gtaggatgca atcgacgatc aggcggaggt agctgtgctg
      181 ttccgtgctg ccgatgggga ggaccgcgcg gtcgtccttc ttgagatagg cctcgacctg
      241 catccaattc atgtcgctga ttcgcacggc gagagtctcg aggttcggag tcggcgagaa
      301 tgtaccgcgc gggtatgcgg cgagccatgc acgtgtgcgg tacggacccc aacacttcac
      361 acgcattgtg cgtacggggc cttcggtgtg ccggcgcggc gtaaagccgc ggctcggcct
      421 ctgcccggtg aacgggcatg aagcgccgct tcatgcggca ggcgccgagc cgcgacttca
      481 ggtcgcgccg gcaacgcgta ggccccggcc tgtcacacct ggcccgcctg cctggtcata
      541 ttcgttgaga tgagcacccc cgacgaccgc gacggccaat tcacggccaa ccggcccctg
      601 ctcttcgcca tcgcctaccg catggtcggc acggtgatgg acgcggagga cctcgtgcag
      661 gacgcctacc tccgctgggt ggaggcgccc gagaccgacg tccgcgtccc ggccgcctac
      721 ctcaccacca tcatcacccg gctcgccatc aacttcctcg gctctgcgcg aaaccgccgg
      781 gaagagtatg tcggaccctg gcttccggag ccgctcgtga ccgatccatc ccccaacccc
      841 gaggcgtccg tcgagctggc cgagtcgctg tcgctggcct tcctggtaat gctcgagcgt
      901 ctcgcgcccg tcgagcgggc cgtcttcctg ctccacgacg tattcgagct cgagtacgac
      961 gagatcgccc cgatcgtcga gaaaaccgag accaactgcc gccagatcct ctcgcgcgcc
     1021 cgcgaacgcg tgagcaccga gcgcccccgg ttcgacgcgg acaccgagcg cgccgaagcg
     1081 ctggtagcac gattcaatca ggcggcggga aacggtgacc tccaggggct cgtggcgatg
     1141 ctggcggagg acatcacgct gtgggtcgat ggcgggggca aggcgaaggg cgccctgccc
     1201 aagccggtcc atggcagcac cccggtggcg aaggtggtga tcaacaccat gaagcggctg
     1261 gcgccccagg gaatcgtctt ccggatggcg cgcctcaatg gccgccccgg gctgattgcc
     1321 tatggcgcgg gcgaagccca atcggcggtc atcttcgaga tgggagccga gcgcatcgag
     1381 aacatctacg tcgtcgcaaa tccggacaag ctgcagagca tcaagaagag cgtcgcgaaa
     1441 tagacctggc ctgtcacaac tgacggcgcc agccggtcat cagggtgtca ttcacacgca
     1501 ctctgaactg aaccggagcg ttacatggaa acctacctgc cccccgtcga gcgcccgaac
     1561 ggcctcgtca tgaagctcgt ctacgcgctg gccaagcggc gtttcggcca ggtactcacg
     1621 cccctcaagg tcatctccgc tcgcatgccg ctggcgttcg gaacctgggt cgggaaggtg
     1681 tcccagctcg accacaagtt gtcgatccag ccggagctga tgtacctcat ccgtcagaag
     1741 gtggcgcaga tcaacgtctg cgaattctgc atcgatatga gccgctgggc ggcgctgcag
     1801 gcgtccaact cgaccgccaa actggacgcg ctcgaggagt tcgaaacgag cccggtcttc
     1861 tcggacgccg agcgtgccgc gctcaattac gtcacgcact tgacccggtt caagaaagtg
     1921 tccccggcgg tcttcaaccg gctggcggag catttctcgg atcgggacat ctgcgagatc
     1981 acctgggtgg tggcaagtga gcacgtctac aacatctcca acatcgggtt gaacattcac
     2041 tcggacgggt tgtgcagaat accctccgcc tccgccgtcg ccggcgcggc gtaaagcggg
     2101 cactccagga cgccccgggg tcagcggtat cttgcatggc gccgtaggaa taacatccgt
     2161 tcaacgccga ggctgccatg cgtctttctg cgatcctcat gtgcctgctc tgcaccgccg
     2221 cgatcaccaa gccgccgttg agcgatcaga agatcgtcga gacgctcgaa aaacagtggc
     2281 tcgcaggcga gcatgaccga gccaccctcg acaccgttct cgcggccgac ttcgtgcatc
     2341 cggtcgcggc cggcgtgttt ctctcgaagg cgcagcatat cgcctgggcc gtcggacacc
     2401 cggcgccgag agaccggcag cagcagttcg accaactgca ggtgcgtgtc tacggctccg
     2461 ctgccgtggt gaccggcatg gtgatttcga ccgatctggc gggtcatgag gcgaagacgg
     2521 tattcacgga tgtctttgca aagcggggcg g
//
LOCUS       DASUBP010000319        16778 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Gemmatimonadales bacterium isolate SMAG_U2524
            k141_1117444, whole genome shotgun sequence.
ACCESSION   DASUBP010000319 DASUBP010000000
VERSION     DASUBP010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37960981
            Sequence Read Archive: SRR25158450
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Gemmatimonadales bacterium (soil metagenome)
  ORGANISM  Gemmatimonadales bacterium
            Bacteria; Gemmatimonadota; Gemmatimonadia; Gemmatimonadales.
REFERENCE   1  (bases 1 to 16778)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16778)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (25-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/11/2024 04:14:25
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,452
            CDSs (total)                      :: 2,426
            Genes (coding)                    :: 2,424
            CDSs (with protein)               :: 2,424
            Genes (RNA)                       :: 26
            rRNAs                             :: 1, 1 (5S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 21
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 2
            CDSs (without protein)            :: 2
            Pseudo Genes (ambiguous residues) :: 0 of 2
            Pseudo Genes (frameshifted)       :: 0 of 2
            Pseudo Genes (incomplete)         :: 2 of 2
            Pseudo Genes (internal stop)      :: 0 of 2
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16778
                     /organism="Gemmatimonadales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1117444"
                     /isolate="SMAG_U2524"
                     /isolation_source="soil"
                     /db_xref="taxon:2448054"
                     /environmental_sample
                     /country="China"
                     /lat_lon="31.23694444 N 118.31 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="VFU75_12190"
     CDS             complement(<1..497)
                     /locus_tag="VFU75_12190"
                     /inference="COORDINATES: protein motif:HMM:NF019268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HEU5467646.1"
                     /translation="MRAPVLLLTAGIAAAPLAAQQFAPPGTKPDSTRVPFAFADFTWL
                     NGNSRQSSSVLDSKYFTGEFRLDASYIYDFNQPSDHTLVGTSESGRTQEVQVQQLGIG
                     GDFHAGHAHARLMTQFGMYSTMTPRNDASPARGQWNLADAYRYISEAYGGFHIDHWNG
                     VNVEAG"
     gene            complement(507..1091)
                     /gene="kdpC"
                     /locus_tag="VFU75_12195"
     CDS             complement(507..1091)
                     /gene="kdpC"
                     /locus_tag="VFU75_12195"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HEU5467647.1"
                     /translation="MLRNQVRPAVIATLVLMLLTGLAYPAAVTGLAQLLFPRQANGSL
                     VTSRGDVIGSDLIGQRFAAARYFHSRPSAAGAGYDDTLSGGRNLGPTSARLDTLVMTA
                     LDSERRLDGPPGGRVPADLVTASASGVDPHISPASAALQVPRVARARGLDPIAVRALV
                     GRFTQGRQFGFLGEPRVNVLLLNLALDSATNQHP"
     gene            complement(1095..3143)
                     /gene="kdpB"
                     /locus_tag="VFU75_12200"
     CDS             complement(1095..3143)
                     /gene="kdpB"
                     /locus_tag="VFU75_12200"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015894702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HEU5467648.1"
                     /translation="MAASKRPLFDPPIVRRAIRDAFAKLDPRHMVRNPVMFVVMVGSV
                     LTTLVLIRDVVSGQRGVGFTLQITLWLWFTVVFANFAEAMAEGRGKAQAESLRKTRTL
                     VPAKRLKDPANRDSGEWAKANELRRGDVVICMPNDLIPGDGEVIEGVASVDESAITGE
                     SAPVIRESGGDRSAVTGGTRVLSDYLIIRITANPGETFVDRMISLVEGASRQKTPNEI
                     ALIILLSGLTIVFLLAVVTLQPFAIYSGSAVSIPILVSLLVCLIPTTIGGLLSAIGIA
                     GMDRMIQHNVIAMSGRAVEAAGDVNTLLLDKTGTITLGNRQAVEFIPAPGITREYLAD
                     RAQLASLPDETPEGRSIVVLAKQFGLRARPLEQTELVPFSATTRMSGINAGRVELRKG
                     ASDAIGRWVRDHGGEMPAPLAAAVDGIAKAGGTPLVVAERDGGAARPLGAIYLKDVVK
                     GGIRERFEQLRAMGIRTVMITGDNPLTAAAIAAEAGVDDFLAEAKPEDKMALIRQEQE
                     GGRLVAMTGDGTNDAPALAQADVAVAMHSGTQAAKEAGNLIDLDSNPTKLIEVVEIGK
                     QLLITRGSLTTFSIANDVAKYFAIIPAMFVTTYPALQALNIMHLHSAESAVLASVIFN
                     ALIIVALIPLALRGVRWKPAAAVAILRRNLWIYGVGGILIPFAGIKLIDMLLVLTRMV
                     "
     gene            complement(3145..4839)
                     /gene="kdpA"
                     /locus_tag="VFU75_12205"
     CDS             complement(3145..4839)
                     /gene="kdpA"
                     /locus_tag="VFU75_12205"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HEU5467649.1"
                     /translation="MTANGWLQIFFFAAAILVVTKPVGVYILRVYDGSFRWLAPVERV
                     VYRLGGVDPADDQHWTRYAAGLLLFSAVSMLVTYFALRLQGSLPLNPFHRAAVTDRQA
                     FETAASFTTNTNWQSYAGETTMSYLSQMMQLTFHNFASAAVGVAAAVALARGVARRSG
                     GRIGNFWVDLVRGTLYLLLPGSLILALLFVQQGVIQNLAPYVTAHTLEGARQVLAMGP
                     VASQEAIKQLGTNGGGFFNANAAHPFENPTPLTNFLSMFAIFVIPAGLTYTFGRMVKN
                     QRHGWALWTAMFVLFAGGVGTAYWAEARGNPIHIAHGVAPDGNMEGKEVRFGIANSAL
                     YATVTTDASCGAVNAMHDSFTPVGGLVPLVNIQLGELIFGGVGSGLYGMIVMAILTVF
                     IAGLMVGRTPEYLGKKIQSREVQMAMLYVLIFPAAVLALAAASVVLPAGLAGLNNAGP
                     HGLSEILYAFTSVTGNNGSAFAGLSGTTTYYNTLLGLGTLFGRFAMIVPILALAGFLS
                     ERKTVPESAGTFPVTTALFVVLLLGVILIVGALTFFPALSLGPIVEHLLMGHGKVF"
     gene            complement(4839..4928)
                     /gene="kdpF"
                     /locus_tag="VFU75_12210"
     CDS             complement(4839..4928)
                     /gene="kdpF"
                     /locus_tag="VFU75_12210"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HEU5467650.1"
                     /translation="MTLETAITGFVALVLLGYLVYTLLRPEKF"
     gene            complement(5056..6459)
                     /locus_tag="VFU75_12215"
     CDS             complement(5056..6459)
                     /locus_tag="VFU75_12215"
                     /inference="COORDINATES: protein motif:HMM:NF017081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
                     /protein_id="HEU5467651.1"
                     /translation="MRFHRPPPAPGPRIAAWIGLATIYIIAGKLGLRVAFLNPSATPV
                     WPPTGIAIAALLLYGRWLWPSILAGAFLVNLTTAGTVFTSLAIAGGNTLEAVVGATLV
                     ARFAGGVGSIRRSSTLVTFFGLAGLGATAIAATVGSLSLVLGGLSPWSGIFWVWSTWW
                     LGDAVGAVVVAPLLLLWARRPRRITRTLVVEGGLLLVVLLLATGMAFDPGSTWAASRA
                     PIEFLCLPPLFWAAFRFGQRITTLGILLVAVIAINGTLHGAGPFVVNGSPNTSLLMLQ
                     AYLGVVAITSLLLAAAAAERQRAERRLRLRSIRDPLTGLLNYRELMGVLGAEIRRCLR
                     TGRGFAILFLDLDGLKAINDRLGHATGSRALCRMADALRDACRAADTLSRYGGDEFAV
                     VLPEGDSDAALQAAGRITARLAADPEPPKITASYGLALFPQDGDTPETLLAKADVALY
                     KMKRNVHRISSPKGRKR"
     gene            complement(6510..7718)
                     /locus_tag="VFU75_12220"
     CDS             complement(6510..7718)
                     /locus_tag="VFU75_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012683494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HEU5467652.1"
                     /translation="MWTFLRHLKLSHWIGLAMIAGLIFGLASPAAAVASQPLATIFLR
                     LIKSLIVPLIFSTLVVGIAGHGDDLKAVGRLFLKSMIYFEVVTTLALAVGLLVVNVIR
                     PGVGVILLSGAPPVGAPPTASGILEHSFPQSFFEAAAGNEVLQIVIWSVIFAIAITQV
                     KAAVKDRMVQLCHDLSDVMFKFTNVVMWYAPIGVGAALAATIGRGGLKALGPLLTLVF
                     TLYLALVIFGLFVLLPAALVARVPVLRFVRAAAEPWMIAFSSASSEAALPLAMERMEE
                     LGVPKKIVAFVIPMGYSFNLDGSTLYLAVASVFAAQAAGIHMSIGQQLFMMLTLMLTS
                     KGVAGVPRASLVILAGTLGTFHLPVEAVTTIFGVDVLMDMARTSVNVVGNCLATVVTA
                     RWEGEFGPPA"
     gene            complement(7765..9702)
                     /locus_tag="VFU75_12225"
     CDS             complement(7765..9702)
                     /locus_tag="VFU75_12225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEU5467653.1"
                     /translation="MTIPLRLLLIEDSEDDAFLLTRELERGGYALQSLRVDSAAELTA
                     ALDRQPWDLVIGDYSMPQFSGLAGLNIIRQRGLDMPFIFVSGTIGEETAVAAMRAGAN
                     DYVMKGQWKRLLPAIARELRDADERRGRRHAEALLRESEASYATLVEHAPLGIYRSNT
                     KGRFLSVNSALVGMLGYGSVAELLALDMNTDLYLDPEQRKVLLERDTFTDREYDEVEA
                     TWKRKDGQPITVQLSVRAIRNAAREVEYYETFVRDVTEQRQLEAQLVRAQKLEAVGRL
                     AGGVAHDFNNLLTVILSYSSLLIEDNEIPPHTLESVQEIKKAADGAAGLTKQLLAFSR
                     QQVLQPQVLDVNSVVQSTQKLLNRLIGEDVKLITNFAPDLGVARLDPGQVGQIIMNLA
                     VNARDAMPTGGILTIETANVEMDITYVRGHPVAQAGHYVMLAVSDTGIGMDEATKERI
                     FEPFFTTKETGKGTGLGLATVYGIVKQSGGFIWVYSEPARGTSFKIYFPRIADGPAAP
                     GHEPHAPIPAPRGTETILVVEDVTAVRGVAREMLERTGYRVLEAGNGDQALRVAAKHP
                     GPIDLLLTDVVMPGISGRVLAKQLAELRPQMRVLYMSGYTDDAVVNHGMLEPGIHYLQ
                     KPFTRDGLARKVHEVLRGPRT"
     gene            9897..10817
                     /locus_tag="VFU75_12230"
     CDS             9897..10817
                     /locus_tag="VFU75_12230"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEU5467654.1"
                     /translation="MTHFVAVAEERSFSRAAKRVNIVQSGLSASIKALEQELGITLLL
                     RTTRRVDLTEAGSVFLAEVRRVETALQGARDAVEGLRGVVRGTVQVGTMQRLAPLFDL
                     PAVLARFRAEHPQVEVVLRQGASGPMLSEVAEGKLDFAFLALLGAQPRGINVTPLGED
                     AMVAVCPMAHRFSRLSAVSLAQVADEPFLDLVAQSSVRTMVDRAFGAAHIERQVAFEL
                     NDLPMLLELVAHGLGVTVLPRFMASDVRGVVCVPIEPAISRWRLVLARRDRHPIGPAA
                     RALLRLILPRAPGEETGGRRSGPTLKVRGG"
     gene            10818..12653
                     /locus_tag="VFU75_12235"
     CDS             10818..12653
                     /locus_tag="VFU75_12235"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEU5467655.1"
                     /translation="MAESGSGAKLSLSVKLPVLIGGLLIAAVVADSTLSYERVRSSAI
                     AMTGARLGTVGSQLAAQLSQSGSQLVALTRQSADSAVITGLLTHPDQNHKAAAASFLR
                     PSTAAGKLVASAQVFDAARRLVWSTGDSVALRIATATTAELIADLTPKDSGVVSRFLA
                     VGDSVIYASASPSVSNGRLVGYLVVWRYLSMTAQARKQASLLIGADARFYVGNTADNI
                     WSDLNRPVSPPPVSVVGDTSVLHYARAGAEGGFMIATARPVRRAPWIILVEFPESSVL
                     APSASFLRDSLLIGAGILALGLIAAWWMSRRLTRPLVQLTHKAEELAELGSSANDGEQ
                     GGDEIVRLSTAFTSMVAHVRESQHELEKRVKTRTSELQERNEELESFAYSISHDLRAP
                     LRAMDGFSQALLEEYGDQLDATGRQYAERVKAGARRMDLLIRDLLAYSKMTRSDIKVG
                     PVELDRVVRSALDQVEGDARARGARVVVEDRMPRVLGHEATLTQVMMNLIANGIKFVP
                     VGRTPEVRVRTEARDRLVRLWIMDNGIGIPPEYHDRIFRVFERLHRTEEYPGTGIGLA
                     IVRKGVERMGGRVGLESEPDRGTSFWIDLPRAEDQAAVVRAAAAP"
     gene            complement(12617..13750)
                     /locus_tag="VFU75_12240"
     CDS             complement(12617..13750)
                     /locus_tag="VFU75_12240"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEU5467656.1"
                     /translation="MLTLRAAKQRVSDASASEPFSLDVEIEQEEGRTLVVVGESGAGK
                     STLLRLLAGLEKPDRGHIALNGETWFDDPRTGGTWVPPWARSIGYVGQDYALFPHLTV
                     RENVAFGLDARGWSRRDLRDRVATVLDRLAISALAERRPREISGGQQQRAALARALAP
                     KPRLLLLDEPLSALDQRTRQEVRGELKAILNEVRCTTVYVTHAPVEALLFGDRIAVLE
                     NGRLTQLGDREDLLRRPRSSYVASLLGVNLFRGTVVQDPGSPTGVVRVRTKDGSLLVP
                     DPGVRGEVFVTVNPRDVTLYLTPPVGSALNIFHGEVAEVVPQPPDGERVRVVLRTRPP
                     LVTEVTQGAAAALNLKAGAWVHAGFKATGTSVYGAAAARTTAA"
     gene            complement(13728..14561)
                     /locus_tag="VFU75_12245"
     CDS             complement(13728..14561)
                     /locus_tag="VFU75_12245"
                     /inference="COORDINATES: protein motif:HMM:TIGR01581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEU5467657.1"
                     /translation="MTPPFRPRLWVWRIAVAVGVALFLALVGLPLVALVVRVPPGDLI
                     RRATSPLVLQALKLSVLTSATATVLVAAFGLPVAYILATRDFPGRRLLETLVELPMVI
                     PPTVAGVGLLLAFGRAGLAGGVLSALGIEIPFTTLAVVIAEAFVAMPFFVSTTAAGLR
                     EVEPRYREAAVTLGATPPYAFRRVLLPLAMPSLIAGGAMTWARALGEFGATITFAGNL
                     PGRTQTLPLAVYIALQSDVRAAVALSVILLIFSFALLLTLRSFSTGWHGTRIYAHPSR
                     R"
     gene            complement(14558..15346)
                     /gene="modA"
                     /locus_tag="VFU75_12250"
     CDS             complement(14558..15346)
                     /gene="modA"
                     /locus_tag="VFU75_12250"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEU5467658.1"
                     /translation="MLIPLACLAALAAAQQAGSPAPRTLTVFAAASLQDAFTALGDSL
                     EHAHPGLKVSFDFSGSQTLAFQIQQGAPADVFASADDRWMTAVKDSGRVRGEPRIFAH
                     NALVVIVPASNPGHITELADLARPALKLVLAADAVPAGKYARVALANLSKLPGYGATF
                     AEHVLANLVSNEENVKSVLAKVRLGEADAGIVYGSDVAQGAADLLTLGIPPGANVVAD
                     YPIAVLTHGPDPADAGTFVALVLSPVGQRVLASRGFTPVSLRAH"
     gene            15443..16444
                     /locus_tag="VFU75_12255"
     CDS             15443..16444
                     /locus_tag="VFU75_12255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEU5467659.1"
                     /translation="MRRLSLLFGAALTLAAPAAAQNYTITHTYKLGGDGGWDYLALDT
                     AGHRLFIARQDRVMVVDPEDGKPIAVIPGLNRAHGIAFAYAEGHGFATSGADSTVTMF
                     DLKTLKVLKRTKAAIDDDAVLFDPATGHIFTMNGDAKSSSVIDAKTGDLIGNIDLGGG
                     PEFGVSAGDGKLYINIEDASEVVEVDAAAMKVTRRWSIKPCESPSGLAIDRVHHRLFS
                     GCRNGTMGISDAQAGKLVTTVPIGRGVDACRFDEGTGLAFASNGDGTLTVIHQDSPDK
                     YTVVGNVTTMGGARTMELDQVSHRLFTVSAEFGPLPAGQRRPPIVPGTFSLLELDRQ"
     gene            complement(16532..>16778)
                     /locus_tag="VFU75_12260"
     CDS             complement(16532..>16778)
                     /locus_tag="VFU75_12260"
                     /inference="COORDINATES: protein motif:HMM:NF025691.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HEU5467660.1"
                     /translation="LSRGLPLVKWFLAIPHYIVLALLGLGVWVCVVVAWFAILLTGRY
                     PRLLFDFVVDVIRWGVRVSAYAFLLTTDQYPPFRLTD"
BASE COUNT         3004 a         5407 c         5728 g         2639 t
ORIGIN      
        1 cccgcctcga cgttcacgcc gttccagtgg tcgatgtgaa aaccgccgta ggcttccgag
       61 atgtaccggt aggcgtccgc cagattccat tgcccccgtg cgggcgaggc gtcgttgcgg
      121 ggcgtcatgg tcgagtacat gccgaactgg gtcatcaatc gggcatgggc gtgcccggcg
      181 tggaagtcgc ctccgatgcc gagctgctgg acctgcacct cctgggtgcg accactctcg
      241 gacgtgccca ccagggtgtg atcactcggc tgattgaagt cgtagatgta gctggcgtcg
      301 agccggaact cgccggtgaa gtacttcgag tcgagcaccg acgaagactg gcggctgttc
      361 ccgttgagcc acgtgaaatc cgcgaatgcg aatggcaccc tagtcgagtc cggcttggtc
      421 ccaggcggcg caaattgctg ggccgccagc ggggccgcgg cgatcccggc agtcaacaac
      481 aggacggggg cacgcacgga gccctcctag ggatgttggt tcgtcgcgct gtcgagcgcg
      541 aggttgagca gcaggacgtt gacgcggggt tcgcccagga agccgaactg ccggccctgg
      601 gtaaatcggc cgacgagcgc ccgcaccgcg atcgggtcga gcccgcgcgc gcgggccacc
      661 cgggggacct gaagggcggc ggacgccggc gaaatgtggg gatcgacccc ggaagcggag
      721 gccgtcacca ggtcggccgg cacgcgtccc cccggaggac cgtcgagcct ccgctcgctg
      781 tctagtgccg tcatcacgag cgtgtccaac ctggcgctcg tgggcccgag gttccgccca
      841 ccggagaggg tatcgtcgta gccggcgccg gccgcggaag gccgggaatg gaaatagcgg
      901 gcggccgcga atcgctggcc gatgaggtcg ctcccgatca cgtcgccacg cgaagtgacc
      961 agggatccgt tcgcttggcg tgggaagagc agctgcgcca ggccggtgac ggccgccggg
     1021 taggccagtc cggtaaggag catcagcacc agcgtggcga tcacggcggg gcggacttga
     1081 ttccggagca tgggctagac catccgtgtg aggaccagga gcatgtcgat gagcttgatc
     1141 ccggcgaagg ggatcaggat ccctcccact ccgtagatcc atagattgcg gcgcaggatg
     1201 gcgaccgcgg cagccggctt ccagcgcacg ccccggagcg ccaaggggat gagggcgacg
     1261 atgatgaggg cgttgaagat cacactcgcc aagaccgccg attccgccga gtgcaggtgc
     1321 atgatgttca gcgcctggag ggcggggtag gtcgtcacga acatcgccgg gatgatggcg
     1381 aagtacttgg cgacgtcgtt ggcgatcgag aaggtcgtca gcgagccgcg ggtgatcagc
     1441 agctgtttcc cgatctccac gacctcgatc agcttggtag gattcgaatc gagatcgatg
     1501 aggtttccgg cttctttggc ggcctgggtc ccgctgtgca tggcgactgc gacgtcggcc
     1561 tgcgccaggg ccggtgcgtc gttggtgccg tccccggtca tcgctaccag acgaccgccc
     1621 tcctgctcct gcctgatcag ggccatcttg tcttcgggtt tcgcctcggc caggaagtcg
     1681 tccacgcccg cttcggccgc aatggcggcc gcggtgagcg gattgtctcc ggtgatcatg
     1741 accgtgcgga tgcccatggc ccggagctgc tcgaagcgct cccggatgcc gcccttcacg
     1801 acatccttga ggtagatcgc tccgaggggg cgggcagcgc cgccgtcccg ttccgcgacc
     1861 accaggggag tgccccccgc cttcgcgatg ccgtcgaccg ccgcggccaa cggggcgggc
     1921 atttcgcccc cgtggtcgcg cacccagcgc ccgatcgcgt cggacgcgcc cttccgcagc
     1981 tcgaccctgc cggcgttgat gccgctcatc cgcgtggtcg ccgaaaaagg caccagttcc
     2041 gtctgttcga ggggccgggc gcggaggccg aactgcttgg ccagcacgac gatgctccgt
     2101 ccttcggggg tctcgtccgg cagtgaggcg agctgagctc gatccgcgag gtactcccgg
     2161 gtgatcccag gcgccgggat gaactccacg gcttggcggt tgcccagcgt gatggtgccg
     2221 gtcttgtcga ggaggagggt gttcacgtcc cccgctgctt ccaccgcccg cccgctcatc
     2281 gcgatcacgt tgtgctggat catccggtcc atgcccgcga tgccgatggc cgagagcagg
     2341 ccgccgatgg tcgtgggaat gagacagacc agaagcgaga cgagaatcgg gatcgagacc
     2401 gcgctcccgg agtaaatcgc gaagggctgc agcgtgacga ccgccagcaa gaagacgatc
     2461 gtcagtcccg agagcaggat gatcagcgcg atttcgttgg gcgtcttctg ccggcttgcc
     2521 ccttccacca gagaaatcat ccggtcgaca aacgtctctc cggggttcgc ggtgatccgg
     2581 atgatcaggt agtcggagag tactctcgtc cctccggtga ccgccgaccg atcaccgccc
     2641 gattcgcgga tcacgggcgc ggattcgccg gtgatggccg actcgtccac cgacgccacg
     2701 ccctcgatca cctctccgtc tccggggatg agatcgttgg gcatgcagat gacgacgtcc
     2761 ccacgacgca gctcgttcgc cttggcccat tcgccggaat cgcggttggc gggatccttc
     2821 agtcgcttcg cgggaacgag ggtcctggtc tttcgcaagg actcggcctg ggcctttccg
     2881 cgcccttcgg ccatggcctc cgcgaaattg gcgaacacca cggtgaacca gagccacaag
     2941 gtgatctgga gtgtgaagcc gacgccgcgc tgcccggaaa cgacgtcccg gatcaggacc
     3001 agggtggtca agacgctccc gaccatcacg acgaacatga cggggttccg caccatgtgc
     3061 cgcgggtcca gtttggcgaa cgcatcgcgg atggcccggc gcacgatggg cggatcgaac
     3121 aggggccgct tgctggcggc catatcagaa caccttgccg tggcccatga ggaggtgttc
     3181 cacgatgggg ccaagggata gagcggggaa gaacgtcagg gcgcccacga tgagaatcac
     3241 accgagcagg agcacgacga agagcgccgt cgtgactggg aaggtcccgg ctgactccgg
     3301 gaccgtcttg cgctccgaca gaaagccggc cagggccagt atcgggacga tcatcgcgaa
     3361 gcggccgaac agcgtgccca gtcccaacag cgtgttgtag tacgtggtcg ttccgctcag
     3421 gcccgcgaac gccgacccat tgttgcccgt gacggacgtg aaggcgtaca ggatctcgga
     3481 aagcccgtgc gggccggcgt tattgagacc cgcgagcccg gcgggaagaa ccaccgacgc
     3541 ggcggccaag gccaggactg ccgccggaaa gatcagcacg tacaacatgg ccatctggac
     3601 ctcgcggctc tggatcttct tgccgaggta ctccggcgtc cgtcccacca tgagaccggc
     3661 gatgaacacc gtcaggatcg ccatgacgat catgccatag agccccgacc cgacgccgcc
     3721 gaagatcagc tcgccgagct ggatgttgac cagcggtacc aggccgccaa ccggtgtgaa
     3781 cgagtcgtgc atggcgttca ccgcgccgca cgacgcgtcg gtcgtgacgg tggcgtagag
     3841 ggcggagttg gcgatcccga agcggacttc cttcccttcc atgttgccgt cgggagccac
     3901 gccgtgcgcg atgtgaatcg ggttcccccg cgcctcggcc cagtacgccg tgcccacgcc
     3961 accagcgaag agcacgaaca tcgccgtcca gagcgcccat ccgtgccgct ggttcttcac
     4021 cattcggccg aacgtgtacg tgaggccggc ggggatgacg aagatcgcga acatcgagag
     4081 gaagttggtc aacggggtgg gattctcgaa cggatgggca gcgttcgcat tgaagaaccc
     4141 cccgccattg gtgccgagct gcttgatggc ctcctggctc gccacgggcc ccatggccag
     4201 cacctgtcgc gcgccctcca gcgtgtgcgc cgtgacgtac ggcgccagat tctggatgac
     4261 gccctgctgg acgaagagca gggcgaggat gagcgaaccc ggaaggagga gatacagggt
     4321 gccacggacg aggtcgaccc agaaattgcc gatccggcca cccgagcggc gggcaacgcc
     4381 gcgcgccaat gccaccgcag cggccactcc caccgccgcc gacgcgaagt tgtggaaggt
     4441 cagctgcatc atctgggaga ggtacgacat ggtcgtctcc cccgcgtacg actgccagtt
     4501 ggtgttcgtc gtgaaggacg cggcggtttc gaaggcctgc cggtccgtga ccgccgcgcg
     4561 atggaaggga ttcagaggca atgacccttg gagccgcaac gcgaaatagg tgacgagcat
     4621 cgatacggcg ctgaacaaca ggagcccggc cgcgtaccgc gtccaatgct ggtcatccgc
     4681 agggtcgaca ccaccgagtc ggtagacgac gcgctcgacc ggcgccagcc agcggaatga
     4741 gccgtcatac acccggagga tatagacccc gacgggcttg gtgaccacca ggatcgcggc
     4801 ggcgaagaag aagatttgca gccaaccgtt ggccgtcatc agaacttctc cggccggagc
     4861 agtgtgtaga ccaggtagcc gagcaggacc agcgccacga atccggtgat tgccgtctcg
     4921 agcgtcatga gccgcgcccc aggcgggcgc atccccggac gtacgccagc atcagtccga
     4981 agaaagcgag ggtggcgagg atgtagagga aatcgcgcac ggtaccagag taccgtccgc
     5041 ggtattaaga cggcgttagc gtttccggcc cttcgggctg gagatgcggt ggacattcct
     5101 cttcatcttg tagagcgcca catcggcctt ggcgaggagg gtttcggggg tgtccccatc
     5161 ctgcggaaag agcgccagcc cgtagctggc agtgatcttc ggcggctcgg gatcggccgc
     5221 gagcctggcg gtgatgcggc cggcggcctg gagtgccgca tccgagtctc cttccggcaa
     5281 gaccaccgcg aactcgtccc caccgtaccg cgagagcgta tccgccgccc ggcacgcgtc
     5341 ccgcagcgcg tccgccatcc ggcagagggc ccgtgaaccg gtggcatggc ccaaccggtc
     5401 gttgatcgcc ttcagcccat cgagatcgag aaagagaatc gcgaatccgc ggccggtgcg
     5461 gaggcaccgc ctgatctcgg cgccgaggac gcccatcagc tcgcggtagt tgaggagacc
     5521 ggtcaacggg tcccggatcg agcgcagccg caaccgtcgc tctgcccgct ggcgctccgc
     5581 ggccgcggcc gcgagcagca gggacgtgat ggcgacgacg cccaggtatg cctgcagcat
     5641 gagcagcgag gtattgggcg aaccgttcac cacgaagggt ccggccccat gcagggtccc
     5701 gttgatggca atcaccgcga ccaacaggat cccgagggtg gtgatccgtt gcccaaaccg
     5761 aaacgccgcc caaaagagcg ggggcaggca gagaaactcg atcggtgccc ggctcgcggc
     5821 ccacgtggag ccgggatcga acgccatccc ggtcgcgagc aacaggacga ccaacaggag
     5881 tccgccctct acgaccaggg tgcgggtgat ccgtctgggg cgccgcgccc acagcaggag
     5941 caggggtgcc accacgaccg cgcccaccgc atcaccgagc caccaggtac tccacaccca
     6001 gaagatgccg gaccacggcg acaatccacc cagaacgagc gaaagcgagc cgaccgtagc
     6061 ggcgatggcg gtcgcgccga gtcccgccag accgaagaag gtcacgagcg tggagctccg
     6121 gcggatcgat ccgacgccac cggcgaaacg ggccacgagc gtcgcaccga ccacggcctc
     6181 gagggtgttg ccccccgcga tcgcgagtga ggtgaagacg gtgcccgccg tggtcaggtt
     6241 gacgagaaac gcgccggcaa gaattgaggg ccaaagccat cggccgtaga ggaggagcgc
     6301 tgcgatggcg atgccggtgg ggggccagac cggtgtcgcg ctgggattca gaaacgcgac
     6361 cctgagcccg agcttccccg cgatgatgta gatggtcgcg aggccaatcc aggccgcgat
     6421 tctggggccc ggggcggggg gaggccggtg aaagcgcatt gttaacgggt gccatacggc
     6481 atggtgccgc ggctgtaacc cccatcacct catgctggtg gtccgaactc cccttcccaa
     6541 cgggccgtga ccacggtagc gaggcagttc cccacgacgt tcaccgacgt tcgggccatg
     6601 tccatcagca catctacccc gaagatcgtg gtgacggcct ccaccggcag gtggaacgtg
     6661 cccagcgtgc cggcgagaat gaccagggag gcccgcggga ctcccgccac acctttgctc
     6721 gtcagcatca gggtcagcat catgaacagc tgctgcccga tcgacatgtg aatgccggcc
     6781 gcctgggccg cgaagaccga cgcaaccgcg agataaaggg tcgagccgtc gaggttgaac
     6841 gaatacccca tgggaatcac gaacgcgacg atcttcttcg gaacgccgag ctcctccatg
     6901 cgctccatgg cgaggggaag cgccgcctcc gaggaggccg acgagaacgc aatcatccag
     6961 ggctcggcgg cagcccgcac gaaccgaagc accggcacgc gcgcgaccag cgcggccggg
     7021 agcaagacga acagtccgaa gatgacgaga gccaggtaca gggtgaagac gagcgtgagg
     7081 aggggaccca gcgccttcag cccgccgcga ccaatcgtgg ccgccagtgc cgccccgaca
     7141 ccgatgggcg cataccacat cacgacgttg gtgaacttga acatcacgtc cgacaggtcg
     7201 tggcagagct ggaccatgcg gtccttcacc gccgccttca cctgagtgat ggcgatggcg
     7261 aagatcacgc tccaaatgac gatctgaagc acttcattgc cggcggcggc ctcgaagaaa
     7321 ctctgcggga acgaatgctc gaggatcccg gaagcggtcg gcggggcgcc taccgggggc
     7381 gcgcctgaaa gcaggatgac gccgacgccg ggccggatca cgttcacgac gaggagaccg
     7441 acggccagag ccagcgtcgt gacgacctcg aagtagatca tggatttcag gaacaggcgc
     7501 ccgacggctt tcaagtcgtc accatgaccc gcgatcccga ccacgagggt cgagaagatc
     7561 aggggcacga tcagcgattt gatgagccgg aggaaaatcg tggccagagg ctgggatgcc
     7621 acggcggcag cgggcgaggc gaggccgaag atcaggcctg cgatcatcgc gaggccgatc
     7681 cagtgactga gcttgaggtg gcgcaagaag gtccacatag gcgcgggaag ctaccgggcc
     7741 cttggtgaat gcgccaagtg cgaattaggt ccgggggccc cgaagcactt catgcacctt
     7801 gcgcgcgaga ccgtcccgcg tgaaaggctt ctgcagataa tgaataccgg gctcgagcat
     7861 gccgtgattg accaccgcgt cgtccgtgta ccccgacatg tacagcaccc gcatctgcgg
     7921 acggagctcg gccagttgct tcgcgagcac acggccggaa attccgggca tcacgacgtc
     7981 ggtcaggagc agatcgatgg gacctgggtg tttggccgcc acgcgcaggg cctggtcgcc
     8041 attccccgct tcgagcaccc ggtacccggt ccgctcgagc atttcccggg cgaccccacg
     8101 gacggcggtc acatcttcga ccaccaggat ggtttcggtg ccccggggag ccggtatcgg
     8161 agcatgcggc tcgtggcctg gcgcggcggg accgtcggcg atgcgcggaa agtaaatctt
     8221 gaatgaggtt ccgcgagcgg gctccgagta cacccagatg aacccccccg actgcttcac
     8281 gatgccgtac accgtcgcga gtccgagccc ggtgcctttg ccggtctcct tggtcgtgaa
     8341 aaagggctcg aagatccgct ccttggtcgc ttcatccatc ccgatgccag tgtccgacac
     8401 ggcaagcatg acgtagtgcc cggcctgggc caccgggtgc ccacggacgt acgtgatgtc
     8461 catttcgacg ttggcggtct cgatggtgag aattccgccc gtcggcatcg catcgcgggc
     8521 attcaccgcc aggttcatga tgatctggcc gacctgtccc ggatcgaggc gggcgacccc
     8581 gaggtcgggg gcgaagttcg tgatgagctt cacgtcctcg ccgatgagcc ggttgaggag
     8641 cttctgggtc gactggacca ccgagttcac gtcgagcact tgcggttgca gcacttgctg
     8701 gcgcgaaaat gccaggagct gcttggtgag tccggccgct ccatccgccg ctttcttgat
     8761 ctcctggacg ctctcgagcg tgtgcggcgg aatctcgttg tcttcgatca gcaggctgga
     8821 gtagctgaga atcacggtga gcagattgtt gaagtcgtgc gccacgccac cggccagacg
     8881 ccccaccgct tccagcttct gggcccggac cagctgggcc tcgagttgcc gttgctcggt
     8941 cacgtcccgg acgaaggtct cgtaatactc gacctcgcgg gccgcattgc ggatggctcg
     9001 caccgacaac tggacggtga tgggttggcc atccttccgc ttccaggtgg cttcgacttc
     9061 atcgtactca cggtcggtga aggtgtcccg ttccagcagg accttccgct gctcggggtc
     9121 caggtacaga tccgtgttca tgtcgagcgc cagcaactcg gccaccgagc cgtatccaag
     9181 catccccacg agcgcgctgt tgaccgagag gaaccggccc ttggtattcg accggtagat
     9241 gccgaggggc gcgtgttcga ccagagtggc gtagctggct tccgactcgc gcaggagggc
     9301 ttcggcgtgc cgccgacccc gacgctcgtc ggcatcgcgg agttcgcgtg cgatggccgg
     9361 gaggaggcgt ttccattggc ccttcatcac atagtcattg gccccagccc gcatcgcggc
     9421 gactgccgtc tcttcgccga tggtccccga gacgaagatg aagggcatat cgaggccgcg
     9481 ttggcggatg atgttgaggc ccgcgagccc cgagaattgg ggcatcgaat agtcgccgat
     9541 gaccaggtcc cagggctgcc ggtcgagcgc tgccgtcagt tcggcggcac tgtcgacacg
     9601 cagagactgc aacgcgtagc cgcctcgttc gagctcacgg gtgagcagga acgcgtcgtc
     9661 ttcagagtcc tcaatcagca ggagacgaag ggggatcgtc atcccgggag tgacctccta
     9721 gggcagacta ggtcttgatt tgactttcga ttgcgtgtga cccgggactc tggtcacgaa
     9781 gcgcttgccc tgcacaatca gtacgcttcc ttatggctat tgtgctgtca agtctataga
     9841 agcgattgca ctaatcactg tagaagatat tttgtatcac tatggaaata cgacagttga
     9901 cgcatttcgt ggcggtggca gaggagcgca gcttcagccg ggcggccaag agagtgaaca
     9961 ttgtgcagtc cggactctct gcctcaatta aggcgctgga gcaggagttg gggatcacct
    10021 tgttgctgcg gaccaccagg cgcgtggacc tgaccgaagc ggggagcgtt tttctggcgg
    10081 aggtccgccg cgtggaaacc gcgctccaag gggctcggga cgcggtcgag ggattgcgtg
    10141 gcgtagtccg cgggacggtg caggttggga ccatgcagcg gctcgctccg ctcttcgacc
    10201 tgccggctgt actcgcacga ttcagagccg agcacccgca ggtagaagtg gtactgcgac
    10261 agggagcgtc ggggccgatg ttgtcggagg tggccgaggg caagctggat ttcgcgttcc
    10321 tcgcgctcct gggggcacag ccgcgcggca tcaacgtgac gccgcttggg gaggacgcga
    10381 tggtggcggt ctgcccgatg gcgcaccggt tctctcggct gtcggccgtg agtctcgccc
    10441 aagtggcgga cgagccgttt ctggacctcg tggcgcagtc cagcgtccga acgatggtgg
    10501 atcgggcgtt cggggcggcg cacatcgagc ggcaggtcgc cttcgagctg aatgacttgc
    10561 cgatgcttct cgagctggtc gcccatgggc tgggcgtcac ggttctaccc cggttcatgg
    10621 ccagcgacgt gcgcggggtg gtatgcgtac ccattgagcc ggcgatcagc aggtggcgcc
    10681 tcgtcctggc ccgtcgggac cggcacccga tcgggccggc cgcccgcgcc ctgctgcggc
    10741 tgatcctacc ccgggcgccc ggggaggaga ccggtggaag acgctccggt ccaacactga
    10801 aagtccgagg aggatgaatg gcggagtccg gcagcggcgc caaactctcg ctctcggtga
    10861 agctccctgt gctgatcggg ggcttactca tcgctgccgt ggtggccgat tcaaccctgt
    10921 cgtatgagcg ggttcgcagc tcagccatcg cgatgaccgg tgcccggctc gggaccgtcg
    10981 gttcccaact ggcggcccag ctctcccaaa gcgggtccca actcgtggcg ctcacgaggc
    11041 agtccgccga ctccgccgtc atcaccggtc tcctcaccca tcccgatcag aaccataaag
    11101 ctgccgccgc cagcttcctg cgtcccagca ctgccgccgg caagctggtc gcctccgcgc
    11161 aagtcttcga tgccgcccgc cgcctggtgt ggtccaccgg tgactccgtg gcgttgcgaa
    11221 tcgccaccgc gacgaccgcg gagctgatcg cggacctcac gccgaaggat tccggcgtcg
    11281 tcagccgctt tctggcggtc ggcgattcgg tgatctacgc gtccgcgtcg ccgagcgtct
    11341 cgaatgggcg cctggtggga tacctcgtcg tgtggcgcta cctctcgatg acggcacaag
    11401 cccggaaaca agccagcctg ctgatcggag ccgatgcccg gttctacgtc ggcaatacgg
    11461 cggacaacat ttggagtgat ctcaatcggc cggtgtcacc tccgcccgtt tccgtggtcg
    11521 gggacaccag tgtcttgcac tacgcgcggg cgggagccga ggggggcttc atgatcgcga
    11581 ccgcccgccc ggtccgccgg gcgccctgga tcatcctggt ggaattcccg gagtccagcg
    11641 ttctcgcacc gtccgcctca tttcttcggg actccctgct catcggcgcg gggatcctgg
    11701 cgctcgggct gatcgctgcg tggtggatga gccgacggct cacgaggccg ctggtgcagc
    11761 tgacgcacaa ggcggaggaa ctggccgagc tggggtcatc cgccaacgac ggcgagcagg
    11821 gaggtgacga aatcgtgcgg ctctcgaccg ccttcacctc gatggtggcg catgtacgtg
    11881 aatcgcagca cgagctcgag aagcgcgtca aaacgcgcac cagcgagctg caggagcgga
    11941 acgaggagct cgaatccttc gcctactcca tctctcatga tcttcgcgcg ccgctccggg
    12001 ccatggacgg cttcagccag gccctgctcg aagagtatgg ggaccagctc gacgccaccg
    12061 ggcgtcagta cgccgagcgg gtcaaggccg gagcgcggcg gatggacctg ttgatccggg
    12121 atcttctggc ctacagcaag atgacccggt ccgatatcaa ggtcgggccg gtcgagctgg
    12181 accgggtggt tcgaagtgct ctggatcaag tcgaagggga cgcccgggcc cgcggcgccc
    12241 gggtcgtggt cgaagaccgg atgccccgcg tactcggcca cgaggcgaca ttgacccaag
    12301 tgatgatgaa cctcatcgcc aacgggatca aattcgttcc ggtgggaagg acgccggagg
    12361 tgcgcgtccg gacggaagcg cgggaccggc tggtccgcct ctggatcatg gacaacggca
    12421 tcggcattcc gccggagtac cacgaccgga tcttccgggt attcgagcgc ctgcaccgca
    12481 ctgaagaata tccaggcacg ggaatcgggc tcgcgatcgt tcgaaagggt gtggagcgga
    12541 tgggggggcg cgtgggcctc gagtccgagc ccgaccgcgg taccagcttc tggatcgatc
    12601 tccctcgcgc cgaagatcag gcggcggtcg tccgggcggc ggccgctccg tagacgctgg
    12661 ttccagtcgc cttgaagccg gcgtgaaccc acgcgccggc cttcagattg agagcggccg
    12721 ccgccccctg ggtcacttcc gtgaccagcg gtggccgcgt ccgcagcacc acccgcaccc
    12781 gctccccatc cggcggctgc ggcaccacct cggccacttc tccgtggaag atgttcagcg
    12841 cgctcccgac ggggggggtg aggtagagcg tcacgtcccg cgggttcacg gtgacgaaca
    12901 cttcgccacg aaccccgggg tccgggacga ggagggagcc gtccttcgtg cggacgcgca
    12961 ccacccccgt gggcgagccg ggatcctgta ccaccgtccc ccggaacaga ttcacaccca
    13021 ggagtgaggc gacgtaggac gagcgggggc gccggagcag gtcctcccgg tctcccagct
    13081 gggtcaggcg cccattctcg agcaccgcga tccggtcccc gaaaagcagg gcttcgacgg
    13141 gggcgtgcgt cacgtagacg gtcgtgcaac gaacctcgtt caggatcgcc ttgagctcgc
    13201 cgcggacctc ctgacgagtg cgctgatcga gcgccgagag aggctcgtcg agaaggagga
    13261 gccgcggctt gggagcgagg gcgcgcgcga gggccgcgcg ctgctgctgg ccgccggaga
    13321 tctcacgggg tcgccgctcg gcgagcgcgg agatcgccag gcgatcgagc acggtggcta
    13381 cccggtccct gaggtcccgg cggctccatc ctcgcgcgtc gagcccgaac gcgacgtttt
    13441 cccgcaccgt gaggtggggg aagagcgcgt aatcctgtcc gacgtacccg atggaccgcg
    13501 cccacggcgg cacccaggtt ccgccggttc gcggatcgtc gaaccacgtc tcgccgttca
    13561 gggcgatgtg tccgcgatcg ggcttttcca ggccggccag caggcggagc agggtagact
    13621 tgcccgcgcc actctcgccc accacgacga gggtccgccc ctcctcttgt tcgatctcga
    13681 cgtcgagcga aaagggctcg ctcgcggagg cgtctgacac ccgctgttta gcggcgcgaa
    13741 gggtgagcat agatccgggt gccgtgccaa ccggtggaga aggatcggag ggtcagaagc
    13801 agcgcgaacg aaaagatcag caggatcacc gagagcgcga cggctgctcg aacgtccgac
    13861 tgcagggcga tgtagaccgc caagggaagg gtctgggtgc ggcccggaag gttcccggca
    13921 aacgtgatgg tggctccgaa ctcaccgagt gcccgggccc aggtcatcgc gcctcccgcg
    13981 atgagtgacg gcatcgcgag ggggagcagg actcgacgga acgcgtaagg cggagtggcg
    14041 cccagggtga ccgccgcctc ccggtacctc ggctccactt cgcgcagtcc ggcggcggtg
    14101 gtggacacga agaacggcat ggcgacgaac gcttcggcga tcacgaccgc gagggtggtg
    14161 aacgggatct cgatcccgag tgccgacagc acccctccgg cgagtcccgc gcgtccgaag
    14221 gccagcagca gcccgacgcc ggcgacggtc ggagggatga ccatcgggag ctccaccagc
    14281 gtctcgagca ggcgccggcc ggggaagtct cgcgtcgcca ggatgtacgc caccggcagc
    14341 ccgaacgccg cgacgagtac cgtcgccgtg gcgctcgtga gcacggagag cttgagggcc
    14401 tgcaggacga gcggactcgt cgcccggcgg atcaggtcgc cgggggggac tcggacgacg
    14461 agcgccacca gtgggaggcc caccagtgcc aggaacaggg cgacgccgac cgcgacggcg
    14521 atccgccaca cccacagacg gggccggaag ggaggggtca atgggctcgg agcgaaaccg
    14581 gtgtgaaccc gcgcgacgcg aggacgcgct gccccaccgg agagagcacc agtgccacga
    14641 aggtccccgc atccgccggg tcgggtccgt gagtcagcac ggcgatcgga tagtcggcca
    14701 cgacattcgc gccgggtgga attcccagcg tcaggaggtc ggcggccccc tgggcgacat
    14761 ccgatccata gacgatccct gcgtcggctt cgcccaggcg caccttggcc agcaccgact
    14821 tcacgttctc ttcatttgac accaggttcg ccaggacgtg ttcggcgaac gtggcgccat
    14881 accccggcag cttcgagaga ttcgccagcg cgacccgtgc gtacttcccc gccggcacgg
    14941 cgtcggcggc gagcacgagt ttcagcgcgg gacgagccag atccgcgagc tcggtgatat
    15001 gtccggggtt cgaggccgga acgatcacga ccagcgcgtt gtgcgcgaag atgcgaggct
    15061 cgccccgcac ccggcccgag tccttcacgg ccgtcatcca acggtcgtcg gcggaggcga
    15121 acacgtccgc cggcgcgccc tgctggatct ggaacgcgag cgtctgcgat cccgagaagt
    15181 cgaacgagac cttcaggccc gggtgggcgt gttcgagcga gtcgcccagc gcggtgaacg
    15241 catcctggag tgacgcggcg gcgaatacgg tcagcgtgcg tggggcaggg gaccccgcct
    15301 gctgggcggc ggccagtgcg gccaggcagg cgaggggaat gagcatgaca caagatatcc
    15361 cctctttcgc aggtctccac cggtcagact cccgtaagac tgccggacta gaattccctc
    15421 cccaccatca tgtggagtac cgatgcgccg cctgtccctg ttgttcggtg ccgccctgac
    15481 gctggccgct ccggctgctg cccagaacta cacgatcacc cacacgtaca agctcggcgg
    15541 agacgggggc tgggactatc tggccctcga cacggccggc caccgtctct tcatcgccag
    15601 acaagaccgc gtcatggtgg tggatccgga agacggcaag ccgatcgccg tgatccccgg
    15661 gctgaaccgc gcccatggca tcgcgttcgc ctacgccgaa ggtcatgggt tcgcgacctc
    15721 cggcgccgac agcacggtca cgatgttcga cctgaagacc ctgaaggtgc tgaagcgtac
    15781 caaggccgcg atcgacgacg atgccgtgct cttcgaccct gcgacgggac acatcttcac
    15841 gatgaatggt gacgcgaaga gttcttcggt gatcgacgcc aagaccggcg acctgatcgg
    15901 gaacatcgat ctcggcggcg gtccggagtt cggtgtgtcg gccggcgacg gcaagcttta
    15961 catcaacatc gaagacgcat cggaggtcgt ggaggtcgat gcggcggcga tgaaggtgac
    16021 gcgccggtgg tcgatcaagc cctgcgagtc ccccagcggc ctcgcgatcg accgggtgca
    16081 tcaccggctc ttcagcggct gccggaacgg gaccatggga atttcggatg cccaggccgg
    16141 gaaactcgtc accacggtgc cgatcggccg gggggtggat gcctgccgct tcgatgaggg
    16201 cacgggactc gcattcgcct cgaacggcga cggcacgctc accgtgatac accaggactc
    16261 gcccgacaag tacacagtgg tcgggaacgt gaccaccatg gggggcgcgc ggaccatgga
    16321 gctcgaccag gtcagccatc gcctgttcac ggtgagcgcg gaattcggcc cgctgccggc
    16381 gggacagcgg cggccgccca tcgtgcccgg aacgttttct ctgctggagt tggatcgcca
    16441 atgaccggca ggaggcaagc aggtccttcg cgcctgcgac ggggcgcgta acgcgccccg
    16501 agcgaaggac cttgtttggt gcccggaccg gctagtcggt gagccggaac ggtgggtatt
    16561 gatccgtggt caggagaaag gcgtaagccg atactctcac tccccagcgg atcacgtcca
    16621 cgacgaagtc gaagaggagc cgtggatagc gtccggtaag aaggatcgcg aaccaggcga
    16681 cgacgacgca cacccagacg ccgagtccca gcagggcgag cacgatgtag tgcggaatcg
    16741 ccagaaacca cttcaccagc gggagccccc ggctcagc
//
